LOCUS       DAZQCJ010000001       118633 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000001 DAZQCJ010000000
VERSION     DAZQCJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 118633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 118633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..118633
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.001"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            121..462
                     /locus_tag="ACHU66_000001"
     CDS             121..462
                     /locus_tag="ACHU66_000001"
                     /inference="COORDINATES: protein motif:HMM:NF019021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFR3388322.1"
                     /translation="MAVLHHDQIQIGDDWTVKLFEFDPEDYKGKVHGWQREAPNEVNE
                     ILKAINTIAKPRHQAILIMSYILPEKIRSAKQAQRLGIAESTYYLAKNEALKEFAGQY
                     RDGSLLQYLDS"
     gene            897..2150
                     /locus_tag="ACHU66_000002"
     CDS             897..2150
                     /locus_tag="ACHU66_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR3388323.1"
                     /translation="MKLQEGVDLHFIDTDQFTTNRIRIRFAAEMSEATVAGRVLVANI
                     FEMGNQEFQTAQAVRRRLAELYGAQFSTSVSKRGRVHCVDVTISYVSPRHLPENEDIT
                     VEILDFLYTCIFRPLKKGRGFDSQIFEVEKTNLINFLQSEIEDNFYHADVEMSKLFYK
                     DPSLQIPRVGRLDLVEKETAESTFQIYRNMLRMDKIDIFVLGKVDREQVKRKLEDFGF
                     TYRNPKLELEYNQEYSNITQEKIERKQARQSILELAYHLQVVYNDVNYPALMVFNGLL
                     GAFSHSKLFMNVREKESLAYTIGSQVSIFSGMLKVYAGISHENRLRVMKLISKQLLDL
                     KCGKFTEEELELTKNMLIHSATLAQDRQNNLIEQVYNQVTLGNRNLSWLDWIEAIKSV
                     SIEDVIRVGQMINLQAVYFMEGTEE"
     gene            2147..3430
                     /locus_tag="ACHU66_000003"
     CDS             2147..3430
                     /locus_tag="ACHU66_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR3388324.1"
                     /translation="MKLVEKKYPYMKESVFYGKVDNGLTVILLPKVDFHETYGILTTN
                     FGGIHTRFTLANGKSVVYPAGIAHFLEHKLFETENEEDIMNEFAKLGASANAYTSFRQ
                     TSYLFSTTQKVLESLSLLQSFVREPYFTEDNVEREQGIIEQEIEMYQDDADYRLFTGI
                     LSSLYPESPLAYDIAGTVESIAAITADDLHENFEVFYHPSNMNLFVIGNFDLEAVWKQ
                     ISSYQVAQMDNPAQSFELAGVQKLPIQEHLSEQFEVSTPKLAVGLRGNDEVDKETIQK
                     YRLSLQFLFAMLFGWTSKRYQQLYEQGKIDSSFQFQLEVTPEYHYLIISGDTQEPITL
                     SSILMKALRKFEDDADVTEDHLQLLKNEMYGDFIRSLNSLEFTASHFVSQYSEYENVF
                     DIPQMIQSLQLDDIREAGRRFIDHCDMTDFTIFPK"
     gene            3512..4369
                     /gene="rodZ"
                     /locus_tag="ACHU66_000004"
     CDS             3512..4369
                     /gene="rodZ"
                     /locus_tag="ACHU66_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR3388325.1"
                     /translation="MRQKSIGEVLRTAREGRSWTFVDLQRITKIQAKYLQALEYNDFD
                     FIANSDDVQSILKAYAEALELDADVLLDAYETNSLVKYYEDGEEELRSSELKRSYKVR
                     KRKKNSYLPLIYLLLATGLIIIFVTYIVHSRLQNRASITPTTTSYSIVEQSVSEASSG
                     TETSSAEQAQSNSTSSSSSVTENVTISGSGSNIVATLKTVTYPVEVTVTAKNTTSWIS
                     LSDTLLEGGVTLTPDNPTVTTTVEEGVTSTTLILGVVKGVEVTVGGQKLNLSALTADT
                     GSITINFEQ"
     gene            4379..4918
                     /gene="pgsA"
                     /locus_tag="ACHU66_000005"
     CDS             4379..4918
                     /gene="pgsA"
                     /locus_tag="ACHU66_000005"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR3388326.1"
                     /translation="MKKENIPNALTIGRILVIPIFILLLTVWDSTISHVLAALIFALA
                     SITDYLDGYLARKWQVVTNFGKFADPMADKILVMTAFIMLVELGFAPAWVVAIIICRE
                     LAVTGLRLLLVENGGTVLAAAMPGKIKTFSQMFAVIFLLLHWNAVGQITLYIALFFTL
                     YSGYDYFKGASFLFKDTFK"
     gene            4915..5751
                     /locus_tag="ACHU66_000006"
     CDS             4915..5751
                     /locus_tag="ACHU66_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFR3388327.1"
                     /translation="MMNIIEVKNLKYKYNQEDERYTLNDVSFHVKQGEWLSIIGHNGS
                     GKSTTVRLIDGLLEAESGDIYIDGDALTIDNVWDKRRLIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGIPLEEMRSRVDEALELVGMTDFKTREPARLSGGQKQRVAIAGVVALRPK
                     IIILDEATSMLDPEGRLDLIKIVREIKERHGMTVISITHDLDEVALSDRVIVMKNGQV
                     ESISTPNELFMREDLVDLDLDRPFTTELASSLRQTGLDLPLRYFTEEELEETLWELIS
                     KK"
     gene            5727..6569
                     /locus_tag="ACHU66_000007"
     CDS             5727..6569
                     /locus_tag="ACHU66_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HFR3388328.1"
                     /translation="MGIDLQEVSYTYQAGTPFEGRALFGVDLEIVDGSYTALIGHTGS
                     GKSTILQLLNGLNVPTEGRVVIDDVVITATSENKDIKQVRKKVGLVFQFPESQVFDET
                     VLKDVAFGPQNFGVSQEEAETIAREKLALVGIDESLFERSPFELSGGQMRRVAIAGIL
                     AMEPSILVLDEPTAGLDPAGRQELMDIFKKLHESGMTIVLVTHLMDDVANYADFVYIM
                     EKGKLVRSGKPVDIFQEVDFLESIQLGVPKITKFAANLERRGFVLERLPITIEEFTGM
                     VTHG"
     gene            6562..7356
                     /locus_tag="ACHU66_000008"
     CDS             6562..7356
                     /locus_tag="ACHU66_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFR3388329.1"
                     /translation="MDKLILGRYIPGDSIIHRLDPRSKLLAMFGFLLMIFWANNLVTN
                     ALLIAFVLGMVVLSRIRLSFFINGLKPMIGIILFTTFFQVFFTPGATILWEFWIFKLS
                     VEGLQQAAIIFVRFVLIIFFSTLLTLTTTPLSLADGIESGLAPLKKFKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQRARGVDFNEGNLIQKVKSVIPILIPLFASSFKRADAL
                     ATAMEARGYQGGDGRTKYRILEWKLADTLAILVMLALAGLLFILKK"
     gene            7488..8042
                     /locus_tag="ACHU66_000009"
     CDS             7488..8042
                     /locus_tag="ACHU66_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009911037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HFR3388330.1"
                     /translation="MNITLKKNLKTTIAGLVAGISLLTAGVVSAETYTVKSGDTLSEI
                     AETYNTTVEKLAEQNKITNLDFIHVGQVIELGDVTVKAVESSIEQTQSTSTSTATVTT
                     STYSSNLSAEDAAAKEWIAMKESSGSYDARNGIYIGRYQLTNTYLNGDYSPENQERVA
                     DAYVASRYGSWSAAKAFWLANGWY"
     gene            complement(8499..9371)
                     /gene="sdaAA"
                     /locus_tag="ACHU66_000010"
     CDS             complement(8499..9371)
                     /gene="sdaAA"
                     /locus_tag="ACHU66_000010"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFR3388331.1"
                     /translation="MFYTIEELVQQADNFSANVAELMIATEIELTGRSREEILTIMSR
                     NLTVMKASIVDGLTESKSVSGLTGGDAAKLDAYMKSGKTLSDSAVLSAARNAMAVNEL
                     NAKMGLVCATPTAGSAGCLPAVLGVAIEKLNLTEEQQLDFLFTAGAFGLVIANNASIS
                     GAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAICFVIKNMLGLICDPVAGLVEVP
                     CVKRNAMGASYAMVAADMALAGIESKIPVDEVINAMYQVGAALPTAFRETAEGGLAAT
                     PTGRRLTQEIFGED"
     gene            complement(9384..10055)
                     /gene="sdaAB"
                     /locus_tag="ACHU66_000011"
     CDS             complement(9384..10055)
                     /gene="sdaAB"
                     /locus_tag="ACHU66_000011"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFR3388332.1"
                     /translation="MTNLKFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFGDEPT
                     EVEFQLYNSFAKTYRGHGTDVALVAGILGMDTDDPRIPDSLDIARERGIKVYWRVNKD
                     SNTPHPNTTRIIIKNDKKSISATGVSIGGGNIQVTELNGFSVNLNMNTPTIIIVHQDV
                     PGMIAKVTDVLSKYDINIAQMNVTREKAGEKAIMIIEVDARQCENSIAEIEKIPHLHN
                     VTFFN"
     gene            10270..11394
                     /gene="mnmA"
                     /locus_tag="ACHU66_000012"
     CDS             10270..11394
                     /gene="mnmA"
                     /locus_tag="ACHU66_000012"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR3388333.1"
                     /translation="MSIDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDD
                     TDENGFCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDV
                     MCNKEIKFKAFLDYAMNLGADYVATGHYAQVSRDEDGTVHMLRGADNGKDQTYFLSQL
                     SQEQLQKTMFPLGHLQKPQVREIAERAGLATAKKKDSTGICFIGEKNFKEFLGQYLPA
                     QPGRMMTVDGRDMGEHTGLMYYTIGQRGGLGIGGQIGGDNEPWFVVGKDLSKNILYVG
                     QGFYHESLMSTSLQASQVHFTRDMPEEFKLECTAKFRYRQPDSQVTVKVKGDKAEVIF
                     AEPQRAITPGQAVVFYDGQECLGGGMIDMAYKDGEACQYI"
     gene            complement(11437..11826)
                     /locus_tag="ACHU66_000013"
     CDS             complement(11437..11826)
                     /locus_tag="ACHU66_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HFR3388334.1"
                     /translation="MSTNLEIFNAYNNALIAGDFAALFETMADDIIWHQPGNHSTSGA
                     KIGKEVLGAHLATFAEKTNGTFKVVTNWVSDNDTLVAANVTFLGTRTDGEELNMNGID
                     LFRMEDGKIKEVWLFSADQTAEDAFWG"
     gene            11952..12278
                     /locus_tag="ACHU66_000014"
     CDS             11952..12278
                     /locus_tag="ACHU66_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR3388335.1"
                     /translation="MKKVSEYGICPFATTQKVLTGKWGLVIIYQLSTGTKRFNELQRL
                     IPGITQTMLTRHLRQLEEDRIISRTVYAEVPPRVEYSLTEMGEKFRSVLDAVEAWGLE
                     YIETLT"
     gene            12420..12881
                     /locus_tag="ACHU66_000015"
     CDS             12420..12881
                     /locus_tag="ACHU66_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFR3388336.1"
                     /translation="MDFRTKVDNQIFGVRATALLIKDGKIFLTKDSKGRYYTIGGAVE
                     VNEVAADAVVREVKEELGIDSHVNQLAFVVENKFTQEGIHFHNIEFHFIVEPIGEIPE
                     EMIEDNLKQACEWIDVDKLVNLDVVPVFLAEKLPNWSGQVTHIMNMKEKTT"
     gene            12878..14794
                     /gene="mnmG"
                     /locus_tag="ACHU66_000016"
     CDS             12878..14794
                     /gene="mnmG"
                     /locus_tag="ACHU66_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR3388337.1"
                     /translation="MTHTFAENYDVIVIGAGHAGVEAGLAASRMGCKTLLATINLDMV
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGRNIDKTYIQMKMLNMGKGPAVRALRAQAD
                     KAEYAAEMKRTVERQENLTLRQTMIDEILVEDGKVIGVRTATNQKFSAKAVVVTTGTA
                     LRGEIIIGDLKYSSGPNNSLASITLADNLKELGLEIGRFKTGTPPRVNARTINYEETE
                     IQPDDEKPNHFSFLSKDEDYLQDQIPCWLTYTNATSHEIINSNLHRAPMFSGIVKGIG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTDEIYVQGLSTSLPEDVQQDLIHSIKGL
                     ENAQMMRTGYAIEYDMVMPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIVAGI
                     NAALKVQGKPELILKRSDGYIGVMIDDLVTKGTVEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGRQVGLVDDERWQVFQIHKNQFDNEMKRLESIKLKPIKETNEKVVAMGFKPLTD
                     ALTAKEFMRRPDVTYADAVAFIGPAAEDLDAKTIELIETEVKYEGYIAKALDQVEKMK
                     RMEEKRIPADIDWDDIDSIATEARQKFKLISPETIGQASRISGVNPADISILMVYLEG
                     RSRSISKNKSKDSH"
     gene            14901..16862
                     /locus_tag="ACHU66_000017"
     CDS             14901..16862
                     /locus_tag="ACHU66_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFR3388338.1"
                     /translation="MKRFRFSTIHFVMIGVILFGLVALVHRFFPIGASTVFALLISLL
                     VLIALFIYQKHSYEFSELEQIEYLNNQANSGLMMLLDKMPVGVIKVRPDSNQVEWFNP
                     FAELIFAQENGEFDQKKLREIIDVGLDEERIYANFSGKRYAVNVDFDQGLFYFFDAST
                     EYSATNSLIGSRPVIGIISVDNYDELEDAVTDSQISQVNSFLAQFVSEFCHDYGIYYR
                     RGTMDRFYFFTDYAVLEKLIENKFSVIDKFREEAKKLDLGLTLSMGLAYGNENHHQIG
                     QVALQNLNMAEVRGGDQVVVKENDESKQPLFFGGGSASSVKRTRTRTRAMMTAVSDKL
                     KSVDAVFVVGHRNLDMDALGSAVGMQYFAQNIMNNAYTVYNPDEMAPDIARAIKKLSD
                     ENCSNLLTVEEAMKMVTFQSLLIMVDHSKIGLTLDKDFYDQFTQVVVVDHHRRDTDFP
                     NNAVLTYIESGASSASELVTELIQFQNDKHHKLNRIQSSLLMAGIMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSLEIQQIAATDFEEYRQINELILSGQRIEANVVIACADDTKEY
                     DNIIPSKAANTILDMAGIEAVFVVTRNTKGYVSISARSHSKINVQRIMEEMGGGGHFN
                     LAAAQVYDQTIAEVCEKLTDKIREEINGN"
     gene            16873..17325
                     /gene="rplI"
                     /locus_tag="ACHU66_000018"
     CDS             16873..17325
                     /gene="rplI"
                     /locus_tag="ACHU66_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR3388339.1"
                     /translation="MKVIFLADVKGQGKKGEIKEVPTGYAQNFLIKKNLAKEATNQAI
                     SALRGQEKSKEKAHAEMIAEAEAIKAKLAEEATLVKFVEKVGPDGRTFGSITSKKIAE
                     ELDKQFGIKIDKRHIQLDHPIRAIGLIDVPVKIYQDVIGIINLSIKEA"
     gene            17357..18712
                     /gene="dnaB"
                     /locus_tag="ACHU66_000019"
     CDS             17357..18712
                     /gene="dnaB"
                     /locus_tag="ACHU66_000019"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR3388340.1"
                     /translation="MAELDELRVQPQDILAEQSVLGAIFLDEGKLVFVREYIEAEDFF
                     KHAHKLIFKAMIALSDRNEAIDATTMRNYLVNHGDLENIGGLAYLAEIISSVPTAANA
                     EFYAKIVAEKSNLRKLIARLTDAVNQAYEGADGSDDIIANAEKALIDVSEGASRSGFK
                     RIDDVLNINFDNLETRAKQTSDITGIATGYPALDAMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLGLTVAIFSLEMGAESLVDRMLASEGVINSRAIRTGHLTQEEWNKY
                     MVAQANLARASIYIDDTPGIKITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGEQEDGGIPNNTVEVIIEKNRSGARGTVELMFQKEYNKFASISKRED
                     G"
     gene            18716..18967
                     /locus_tag="ACHU66_000020"
     CDS             18716..18967
                     /locus_tag="ACHU66_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFR3388341.1"
                     /translation="MSDAFTDVAKMKQIKQDIKDHEGQIVELTLENGRKREKNKQGRI
                     VEVYQSLFIVEFEDPNNTFVESYTYSDILTEKILIHYLD"
     gene            19178..19789
                     /gene="rpsD"
                     /locus_tag="ACHU66_000021"
     CDS             19178..19789
                     /gene="rpsD"
                     /locus_tag="ACHU66_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR3388342.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKIKQGTLGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVEVGQVISVREKSIKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            19856..20305
                     /locus_tag="ACHU66_000022"
     CDS             19856..20305
                     /locus_tag="ACHU66_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HFR3388343.1"
                     /translation="MWRKYFLLVLLAILVVGGAFFLSQSAATPNESDYVKAVKSYQAI
                     SIDEVEQKVQDEEEFILYIGRETCPYCRDFVPKLTEAVERSRATIYYLDSESDPNGKI
                     QQFRQSQGLATVPSLTYYKSGKLSGILRKGSQATLSEIENFLALQGQ"
     gene            20438..21955
                     /locus_tag="ACHU66_000023"
     CDS             20438..21955
                     /locus_tag="ACHU66_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFR3388344.1"
                     /translation="MKKKAWLGLLMSLLAVLFLVACGGKSEKTATSSSSTTSSSSEEA
                     VSGASEKVYTDPSELKDSYDVIVVGSGGAGMSAAISAKDAGASVALLEKMPVIGGNTA
                     KSSAGMNASQTKFQEAEGIADTNDKFYEETLKGGKGTNDPELLRYLVDNSASAIDWLD
                     GMGITLSNLTTTGGMSEKRTHRPADGSAVGGYLVNGLYHNLVERKVPIFVNADVTKLQ
                     DKDGAVTGVEVKIAGETKQISAKAVVLATGGFGADLEMVAKLNPALKGYVTTNQEGST
                     GDGIALAESEGAATVDMEQIQIHPSVEQETSYLITEAVRGEGAILVNSKGERFVNELE
                     TRDKVSAAINSLDPNYAYVIFDDALKDRVKAIAQYEEKGLVKTGATLADLAKEIDLPA
                     DTLQTTLDTWNKAVADKNDAAFGRTSGMDHALNTGSYHAIKVAPGVHHTMGGVKINTN
                     TEVLKTDGSAISGLYAAGEVTGGVHGQNRIGGNAVADIIVFGRQAGEQAAGFAKK"
     gene            22041..23405
                     /locus_tag="ACHU66_000024"
     CDS             22041..23405
                     /locus_tag="ACHU66_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR3388345.1"
                     /translation="MKKKIVLLLGAIAVIVLTWSAIAGQRQQSYAEAELRDQDKIWII
                     TDLHYLSQDLFDDGEAFSYIEKTAAGKDLRYGKERMEALVEQVGREHPSLLLVSGDLT
                     LNGEKQSMVELAQYFTEIEEQGTEVLVIPGNHDIASGWARAFKGDQQIVTDQVTAQQF
                     AELFAHHGYQQASSRDKASLSYLAKPFSNAWFLMIDSNIYSDGYGKGAPPTNGRINKE
                     TLEWIEIQLQAAKETGVSLIPVVHHNVLQQHAMLSKGYTLDNAADLKTLFNQYGIQFG
                     FSGHTHSQNIVKEDLGQVNYTEVVNGAFSIYPAIIGQLSLDDTSIHYQKTQLDMASWA
                     DKHQPSDPNLQDHVTYLQTVFDNTSDIMVHNMLNDEGWYDGETADAISQFIMPANRAY
                     FSGEKLDQTWLDEKVFPSQAYQTLQAASPRSFLADYLDVIIKRSQNRDVEKLVLKTEK
                     DFVQ"
     gene            23569..24582
                     /locus_tag="ACHU66_000025"
     CDS             23569..24582
                     /locus_tag="ACHU66_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR3388346.1"
                     /translation="MTLLSIAIPSYNSEAYLHYCVHSLVMGGDKVEILIINDGSSDRT
                     QEIAEELANQFSNVRAIYQENKGHGGAVNTGIREAKGKYFKVVDSDDWVDTRAYLKIL
                     ESLQALEDENTPVDAFISNFVYEKEGQSRKKSMSYQSTLPENRIFGWEDVGAFSKGQY
                     MMMHSLIYRTDLLREVGLVLPEHTFYVDNIFVFTPLQAVKTMFYLPVDFYRYFIGRND
                     QSVNESVMIKRIDQQLKVNRILVDNLDLEAIDNPDLRSYLLNHVEITTIISCALLNRA
                     GTAEHMMKKQELWHYIRDNNPALFKIVRKGLLGQLTNLYGYPGRKIFNAVYKIAKRIY
                     GFN"
     gene            24652..25563
                     /locus_tag="ACHU66_000026"
     CDS             24652..25563
                     /locus_tag="ACHU66_000026"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR3388347.1"
                     /translation="MQRLGQTGLEVSRIGLGCMRMASLTEQETAKVLDTVVGQGINFF
                     DHADIYGGGESEIRFAQGAKLAGLKREDLLLQSKCGIRKGYFDFSKDYILESVDGILK
                     RLDTEYLDVLALHRPDALMEAEEVAEAFYLLKKAGKVHHFGVSNQNIYQMELLQSYFD
                     QPLVVNQLQLSPAHTPLIDAGLHVNMKDDAATMRDGGLIDYCQLKKITIQAWSPFLID
                     LQRGIFANHPNYALLNQTIGEIAERYNVSHETIVVAWILRHPAKIQTIVGSMNPERLK
                     KIAQASQIILTRPEWYDIYRSAGNILP"
     gene            25573..26025
                     /locus_tag="ACHU66_000027"
     CDS             25573..26025
                     /locus_tag="ACHU66_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR3388348.1"
                     /translation="MQWEIKAFDQLSLQELYTILTLRTDVFVVEQACPYPEIDGKDPS
                     CLHLLGTDNGELVAYLRILPAGLSYDEVSIGRVVIKPSHRGKGLGRPMMEQAIAYITT
                     EWKESQIKIGAQAYLEKFYQSLGFEPISEVYLEDDIPHLDMLYRKLVV"
     gene            26120..27871
                     /gene="aspS"
                     /locus_tag="ACHU66_000028"
     CDS             26120..27871
                     /gene="aspS"
                     /locus_tag="ACHU66_000028"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR3388349.1"
                     /translation="MKRSMYAGRVRKEHVGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPESVEAEVMAKAESLRSEFVIEVTGTVVEREQANDNIPTGAVELQVTSLTV
                     LNTAKTTPFEIKDGIEASDDTRLRYRYLDLRRPEMLNNFKLRAAVTHSIRNYLDELEF
                     IDVETPMLTKSTPEGARDYLVPSRVSKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLNEVEIQDIVEGLIAKVLKDTKGIDVTLPFPRMAY
                     DHAMNFYGSDKPDTRFEMLLQDLTELGKEVDFKVFSEAPVVKAIVVKGAADCYSRKDI
                     DKLTDYAKQFGAKGLAWVKVDKGELAGPVAKFLTGITENLTASLQLEDKDLVLFVADE
                     LEVANNTLGALRNRLAKEQGLIDESKFNFLWIVDWPMFEWSEEEGRYMSAHHPFTLPT
                     EETAHHLDGDLAQVRAVAYDIVLNGYELGGGSLRINQKDMQERMFKALGFSAEDAHEQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEDNIREVIAFPKNNKASDPMTQAPSTV
                     ASAQLEELALNITLENE"
     gene            27864..28796
                     /locus_tag="ACHU66_000029"
     CDS             27864..28796
                     /locus_tag="ACHU66_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFR3388350.1"
                     /translation="MNKFFRKQRVRFLRKARKNKFWSVILGISREKYAERISGSIIYG
                     LLSSIAVNFFFRPGNVYSSGVTGLAQIVSALAASYAGWNVPIAVVYYGLNIPLLILAW
                     YKIGYKFTIFTFITVSFSSFFIQFMPPVTLTTDPLVNAIFGGLMLGTGIGFALRNNVS
                     SGGTDIVSIIIRKKTGRQVGSISLIVNIMIMLIAGMTFGWQYALYSMVALFISSRMTD
                     AIFVKQKRMQAMIITNHPERVIKKIHKKLNRGVTIIDGAEGAYNHEQKTILITIITRA
                     EFNDFKHIMKKADPKAFVSVADNVNIIGRFVESD"
     gene            28873..29325
                     /gene="nrdI"
                     /locus_tag="ACHU66_000030"
     CDS             28873..29325
                     /gene="nrdI"
                     /locus_tag="ACHU66_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFR3388351.1"
                     /translation="MKKKIYLVYISLSGNTESFVKRLKSFFQFQTDWEIELVHVKDLV
                     KQDIPFYQLDAPFVAFLPTYLEGGNGVDNGDVEILTNPLGDFIALGTNAELCLGVVGS
                     GNRNFNNQYCLTAKQYAERFNFPVIDTFELRGMQNDIERIGRKIMELM"
     gene            complement(29479..31530)
                     /gene="pulA"
                     /locus_tag="ACHU66_000031"
     CDS             complement(29479..31530)
                     /gene="pulA"
                     /locus_tag="ACHU66_000031"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HFR3388352.1"
                     /translation="MALRQFQASLDDVATIRLVMEKRFDSEHMSFSLESNDATSQLFI
                     HSCLEVDNLVIYYLTSLHALTLDKDYTIYDQDRNKTELGYGHIVRSTLFEQSYTYNGQ
                     DLGATYRPEATTFKLWAPISKQVFLVLEGNPHAMTKGAKGVWEITVEGDLEAHSYHYL
                     HKVNSEWISVHDPYALSSTANSGDSYVINPDKLHKIRRAKTQRPISQAIIYEMSVRDF
                     SWQKEAGFKHRSQFLGLTESPTMDGMKLGMDYIRELGVTHIQLLPVYDFGSVDENKPQ
                     AVYNWGYDPMQYNLPEGSYSSQPNDPYARILELQEAIQAYHDADISVIMDVVYNHVYH
                     AEKYAFELIVPGYFYRYDDHGMRTDGTFCGNDVASERSMVRNYIKQSLRQWLELYGFD
                     GFRFDLMGILDSETINQIQIELSAIHPNVYLYGEGWKMATGLEFEKLAHQYNAEQLPE
                     ISFFNDDYRDTFKKLLLNPNRLVDKQLHEKVQHLLTGSRLSHFLTPQQSLNYIECHDN
                     ATAFDYFHIENPNWTPQQQKRAASFGLQLILISQGMAFIHSGQEFFRTKDEIDNTYNV
                     PDRINRLDWTRAIRYKEHIEFIQELITFRKNNPILSQDNYESIQETCDFYWLTEYVLR
                     YQVTSEEKTIQFIINFADSDFTYEREKDKAVLYNYPPIEERDQQEITIAGQSICILED
                     K"
     gene            complement(31554..32537)
                     /locus_tag="ACHU66_000032"
     CDS             complement(31554..32537)
                     /locus_tag="ACHU66_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR3388353.1"
                     /translation="MRATIKDVAKLAGVSPSTVTRVIQNSSAISQKTKDLVRKAMADL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDVFYQNPFFPTALRGISQVAADHNYAIQIST
                     GKNEEQRLEAISQMVYGKRVDGLIFLYSKPDDPLVQLAIQHKFPFLILGKADSPFISL
                     VDNDNIQAGFEATNYFINKGYKNIAFVAGNKELVVSQDRYTGYKNALKSHNIPLDENK
                     VKFVSGFLLEDSAYKISKKLLKQDLDAIVTTDTSVAEGVVKYLNEVGVKLPIISFDSV
                     KPKLDIEAYVDVHAIKLGRVAFNTLHQIINDNKEDKQVCYRRVIPHTITEL"
     gene            complement(32547..33278)
                     /locus_tag="ACHU66_000033"
     CDS             complement(32547..33278)
                     /locus_tag="ACHU66_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388354.1"
                     /translation="MFANRRLMNLWQRLFTTLFLVALLLIPSSLQTVNLETYPLENFV
                     EGVYAPLTDQVVDDLRQYAVIENNHLTYTGNTNWEQVTFGDTVSSSSDFTYQFANQSL
                     TIRKGSDILSELTYEGLNTDDFASKETLTATISKKWYQANRAAVGLSITLISTFILTT
                     KLLFILLGASFFLFLTKKSRFFHFETFAECYNFALNCLGLPTLIACLIGLFGQPVTTL
                     LTIQNILFVLCLLWVFFKTKFRDEI"
     gene            complement(33389..34231)
                     /locus_tag="ACHU66_000034"
     CDS             complement(33389..34231)
                     /locus_tag="ACHU66_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFR3388355.1"
                     /translation="MKVSVKTRRILNQTLTYLYLIVLSVIIIYPLLITIMSAFKSGNV
                     VAFKLDSNINFTLDNFSKLFSETLYGTWYFNTLIIALLTMVIQTSIVVLAGYAYSRYN
                     FIARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNQSWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPISLDESAKLDGAGHFRRFWQIVLPLVRPMIAVQALWAFMGPFGDYILSKF
                     LLREKEFYTVAVGLQTFISDVKNMKIAYFAAGAILIALPISILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(34233..35528)
                     /locus_tag="ACHU66_000035"
     CDS             complement(34233..35528)
                     /locus_tag="ACHU66_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFR3388356.1"
                     /translation="MTMKQNPGKALLLSLIPGLGQIYNKQKAKGYIFLGVTLAFLVYF
                     IAIAAGELGNLITLGSVRGQDNSLFMLIRGSFHLIITVVYLAFYALNLKDARDTAKRW
                     NSGMPVATTLKEMVKGIYENGFPYLLIIPSYIAMTFAIIFPVVVTLFIAFTNYDFKHL
                     PPSALLDWIGITNFTNIWKLSTFRTAFGSVLGWTIIWALCASTAQIVIGILTAIIANQ
                     PFIKGKRIFGVIFLLPWAVPAFVTILTFSNMFNDSVGAINTQVLPFLSKFLPFIDNFL
                     IPWKTDPFWTKVALIMMQAWLGFPYIYVLTLGILQSIPNDLYEAAYIDGASAIQKFRN
                     ITLPMILAVAAPTLISQYTFNFNNFSIIYLFNNGGPGSVGGGAGSTDILISWIYKLTT
                     GTSPQYSMAAAVTLIISMIVISISMIAFKKLNAFEMEDV"
     gene            complement(35741..37000)
                     /locus_tag="ACHU66_000036"
     CDS             complement(35741..37000)
                     /locus_tag="ACHU66_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR3388357.1"
                     /translation="MKHNLLKSVALLAASTAVLAACSNSGSSTEASKSAEGSKELTVY
                     VDQGYESYINDVKAGFEKENGVSVTVKTGDALTGLDNLSLDNQSGSAPDVMMAPYDRV
                     GSLGSEGQLSELTLADDSKADDTTTALVTNGGKVYGSPAVIETLVLYYNKDLLTEAPK
                     TFAELETLAKDSKYAFAGEEGKTSAFLADWTNFYYTYGLLSGYGGYVFGENGTNPKDI
                     GLANEGAIKAIEYAKTWYEKWPQGLQDGTAANNLINTQFTDGKAAAIIEGPWKAASYK
                     EAGVNYGVATIPTLVNGKNYSAFGGGKAWVVPAGAKNQEMAQKFVDFLTATDQQKALY
                     DATNEVPANTEAREYAVSKKDELTTAVINQFASAQPMPNISEMGSVWTPAGNMLFEAA
                     SGSKDAKTAATDAVKAIADEIAQKHSN"
     gene            37304..38821
                     /gene="malQ"
                     /locus_tag="ACHU66_000037"
     CDS             37304..38821
                     /gene="malQ"
                     /locus_tag="ACHU66_000037"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR3388358.1"
                     /translation="MKVRQSGVLMHISSLPGKYGIGSMGQSAYEFVDFLVRTRQRYWQ
                     ILPLGTTSYGDSPYQSFSAFAGNTHFIDFDILIDKGWLAAEDLHGVDFGQNPTEVDYA
                     AIFYARRPLLEKVVVNMKAQGLPEDYWMFLGENDFWIHTFAEYMAIKEHFDLKPWTEW
                     EDQGARLRYHDTLEYYRHLLADRIDYHRITQYLFFSQWKALKAYANAKGVEFVGDMPI
                     YIAADSADMWANPHFFKTDEEGKPSVVAGCPPDAFSEIGQLWGNPIYDWEAMKHDGFT
                     WWVARLRESFKIYDMVRIDHFIGFASFWEIPAGEETAVNGYRVEAPGFELFDTIKREL
                     GDLNIIAEDLGSVTDKVIQLRDTTGFPGMKVLQFAFDPEGDSIEMPHNHPNNVVAYTG
                     THDNDTILGWYENETTKESRAFLDKYSNRREGETVSHALFRLLFGSPAFMAVATMQDL
                     LGLDGSARMNLPNTLGGNWTWRMTADQLTPEIEAELLDLTETYRRVNVHLVDEKS"
     gene            complement(38862..39044)
                     /locus_tag="ACHU66_000038"
     CDS             complement(38862..39044)
                     /locus_tag="ACHU66_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388359.1"
                     /translation="MKKLPTYIGVIIFVVTLLINWLLPSLAKVLNTFFIWTIVACLLY
                     TIYYWIRYWLYVRQQR"
     gene            39156..39680
                     /locus_tag="ACHU66_000039"
     CDS             39156..39680
                     /locus_tag="ACHU66_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388360.1"
                     /translation="MKRPIHLYIFVVLSSIASVLRVFNVFFAKYNEEAVRQLLQNFNV
                     EGLDEVYFTYMIESVNFQTNLVNKAFAVVLLLAVIATIVLLFLKKNEQASYTYLGYLF
                     VTLLFSTYAFIGEKGLSQIYTDSVMRQSVEAQAMMNYIIRVVLFAIYFGVTVFFHLRK
                     PKEKPSTAINSTDI"
     gene            complement(39734..40648)
                     /locus_tag="ACHU66_000040"
     CDS             complement(39734..40648)
                     /locus_tag="ACHU66_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFR3388361.1"
                     /translation="MKTFLRRKGIACQLLAAIFIVGINLQSPPSASANARGDDYPYTV
                     VDAVDPWRLYTRQCTSFVAFRLSTVNGFTLPPAYGDANVWGHRAKQEGYQVNMTPAVG
                     AVAWWETPMHVAWVSAVNGDTVEIEEYNYGVRYTYGRRVIPKNAVSGYIHFKNLGAGT
                     TNSQPAVAPSGTYTFTSQLAIKSQASLSSPTLDYYYAGESVRYDKVLEADGQRWISYI
                     SYSGARRYIAIQPTITQSTNNSLPASGSYTIQSYSSVRNNPSLSSPEITFYGAGSVVR
                     YDKVITAEGRTWISYISYSGARRYIAIS"
     gene            complement(40828..42516)
                     /gene="argS"
                     /locus_tag="ACHU66_000041"
     CDS             complement(40828..42516)
                     /gene="argS"
                     /locus_tag="ACHU66_000041"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR3388362.1"
                     /translation="MNQKQVIAERLAAILPSLEVEAIYNLLEKPKSSEMGDIAFPAFS
                     LAKVERKAPQAIAADIVEKLDTTGFEKVVATGPYVNFFLDKAAISHQVLTDVITEKDQ
                     YGKLNIGQGRNVTIDMSSPNIAKPFSVGHLRSTVIGDALANIHEKLGYKPIRINHLGD
                     WGKQFGMLIVAYKLWGDKAAVEADPISELLKLYVRINAEAEEKPELDDEARQWFKKLE
                     DGDSEAHELWQWFRDESLVEFNRIYDKLDVTFDSYNGEAFYNDKMDEGIQILEEKGLL
                     QESKGARIVDLESYNLPPALIMKTDGATLYITRDMATAMYRKRTYDFVKSIYIVGQEQ
                     INHFKQLKAVLKEMDFDWSDDMTHVTFGLVTKDKKKLSTRKGNIILLEPTLDEAISRA
                     LTQIEAKNPDLENKEEVAHAVGVGAVKFYDLKTDRDNGYDFDLEAMVSFEGETGPYVQ
                     YAYARIQSILRKANFVPSAENDYKLADAESWDIIKHIQNFSNVVERAGDKFDPSLIAK
                     YAINLAQAFNKYYAHTRILDESPERDSRLALAYATGLVLKEALRLLGVKAPEKM"
     gene            42623..43063
                     /gene="argR"
                     /locus_tag="ACHU66_000042"
     CDS             42623..43063
                     /gene="argR"
                     /locus_tag="ACHU66_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFR3388363.1"
                     /translation="MNKAGRHDLIKAMIRQEKIGRQTDIQQGLEARGVVVTQTTLSRD
                     LRELGVIKIHENGRSFYSLAIEEERVNFIQLLAQYAYKVDRASFILVLHSELGEAALM
                     ANIIDAEKPETILGTLAGANTLLVICRDDVAAQQVEAEINYYLN"
     gene            43078..45618
                     /gene="mutS"
                     /locus_tag="ACHU66_000043"
     CDS             43078..45618
                     /gene="mutS"
                     /locus_tag="ACHU66_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR3388364.1"
                     /translation="MATEKISPGMQQYLDIKAQYPDAFLLFRMGDFYELFYEDAVEAA
                     QILELSLTSRNKNAENPIPMAGVPYHAAQQYIDTLVELGHKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVTDSSKMGADSNYLVALDRQGVQFALSYMDVSTGQFFVTSLDDFTS
                     LCGEIRNLRARELVIGYALSEEEEQVFSNQMNLLLSFEDEVTEDVQLIDNSLTDLEKA
                     AAGKLLSYLHRTQMRDLSHLQKVVHYEIKDYLQMDYATKSSLDLLENGRTGKKHGSLY
                     WLLDETKTAMGMRLLRTWIDRPLIDLKRIENRQAIVQVFLDYFFERSDLVEALKGVYD
                     IERLASRVSFGKTMPKDLLQLSQTLGNIPAIKNILQQINEPALGNLVAGLDPIPELHA
                     LISSAIDPEAQGTITDGNIIRTGFDETLDQYRLVIREGAGWIAEIEAKEREASGINNL
                     KIDYNKKDGYYFHVTNSNLGNVPDHFFRKATLKNSERYGTEELAKIEGQMLEARDKSA
                     NLEYEIFMRIRQEVEKYIGRLQKLARTIATIDVLQAFAVVAEQQHLVCPRFTDQRELT
                     IDRGRHAVVEKVMGKQTYIPNSIHLNTDTHMQLITGPNMSGKSTYMRQLAVIVIMAQM
                     GSYVPADQAELPIFDAIFTRIGAADDLVSGQSTFMVEMMEANKAVRLATDRSLILFDE
                     LGRGTATYDGMALAQSIIEYIHDKIGAKTLFATHYHELTDLSQTLEHLENVHVSTLEK
                     DGQVTFLHKIAQGPADKSYGIHVAKIAGMPEELLQRADRILQTLENQAPTAPTTPAPI
                     VVEEPSGQIDLFADTPSHPVLDELAKLDIYNMTPMEVMMAVAELKKKI"
     gene            45871..46044
                     /locus_tag="ACHU66_000044"
     CDS             45871..46044
                     /locus_tag="ACHU66_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388365.1"
                     /translation="MKIILTIVYSLGVVWNILFLAVNVGRIPVINLLWPAVILVACAI
                     NLYFLWTRKQESE"
     gene            46158..46844
                     /locus_tag="ACHU66_000045"
     CDS             46158..46844
                     /locus_tag="ACHU66_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFR3388366.1"
                     /translation="MGKPIIGITSNEKPVAEGIPIMNLSLSRNFADGIKRAGGLPFYI
                     PLSGETDVVDYVSAIDKLLLTGGQHVLPQFYGQEKLIESDDYFKERDEFELKLVHEAL
                     RQEKPILGICRGMQLFNVARGGTLKQAVEGHRSLPFDLIHQVECKEATPLASIFGQRV
                     AVNSIHSQAIENLGQGLEAIVHSVDDGIIEAVRSMDETRFLGVQWHPELLIDQSGGNQ
                     KLFDYFVQTL"
     gene            46877..47728
                     /locus_tag="ACHU66_000046"
     CDS             46877..47728
                     /locus_tag="ACHU66_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR3388367.1"
                     /translation="MKLRTILSLAGITALATVALAACSSTSNSSNTSSSEADKKETIV
                     MGTVGTTKPFSYRDENGELTGFDIELARAIFDGSDQYELTFETTDGSGQFPGLDSGRF
                     DLLGNNTSYTKERGEKYLYSYPTASTPSVLAVPKDTDIQSYDDIGGHSTQVVTGTTTA
                     AQLEEYNTQHTDKPVDINCTGENITQILTNINDGKYDFKIFDAPTVNAIIADQNLTNL
                     KTIELEAAEKPFIYYVFSSDNTALQSFVNDRIKALNEDGTIAKLTEQFFGKDYAPTAE
                     ELAVPNE"
     gene            47914..48717
                     /locus_tag="ACHU66_000047"
     CDS             47914..48717
                     /locus_tag="ACHU66_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR3388368.1"
                     /translation="MIGVLGITGPAWYQGFLEWLPDGKLFSIRAVFDALPSIIERLPI
                     TIGLTLAGAFFGLILGLVFAIVKINRVRFLYPVQAVFVSFLRGTPILVQLMLTYYGIP
                     LVLRGLNQEYGLDWNINAIPAAVFAITAFAFNEAAYMSESIRAAIQAVSPGEIEAARS
                     LGMTSGQVYRRIIIPNAAVIATPSLINTLIGLTKGTSLAFSAGIVEVYAQAQIQGGAD
                     YRYFERYISVSLVYWALSVLIESFGRFLENKMAIQAPESVDISGVGGDR"
     gene            48717..49460
                     /locus_tag="ACHU66_000048"
     CDS             48717..49460
                     /locus_tag="ACHU66_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR3388369.1"
                     /translation="MIKIRNLVKEFSGQKVLDGIDMDIEKGQVIALVGPSGAGKSTIL
                     RSLNYLEQADAGSIEIDGFTVDFTNISKEEILTLRRKLAMVFQQFNLFERKTALDNVK
                     EGLKIVKRMDDASATALAKEELRKVGLSERENHYPRHLSGGQKQRVALARALAMQPDI
                     LLLDEPTSALDPELVGEVERSIADAAKTGQTMILVSHDMNFIYQVADKVLFLENGRIL
                     EQGTPEELFHHPKEERTREFFASYSNPQF"
     gene            49766..51937
                     /gene="nrdD"
                     /locus_tag="ACHU66_000049"
     CDS             49766..51937
                     /gene="nrdD"
                     /locus_tag="ACHU66_000049"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR3388370.1"
                     /translation="MNVQENVLPSIELLVLKRDGRTVSFDQDKIFSALRRANQELEHP
                     VSEAGLKIVLEAVLAEIGRRFEKDVQIYEIQTVVEQELLKAHLYDLAELYIQYRTRRD
                     FRRHQATDINFEIHKLLSKDQSVVNENANKDADVFNTQRDLTAGIVGKSIGLKLLPAH
                     VANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLAQGFKIGNADVESPKSIQTATAQ
                     ISQIIANVASSQYGGCSADRIDEVLAPYAELNYQKHLKDAKDWVLPDKQEEYARAKTQ
                     KDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSWFEREIQKAILNIRIKGLGREG
                     RTAIFPKLIFTVKRGLNLEPDSPNYDIKQLAIECATKRMYPDMLSYDKIVELTGSFKV
                     PMGCRSFLQGWKDENGQDVTSGRMNLGVVTVNLPRIAMESAGDKDKFWEIFVERMAIA
                     KDALVYRVERVKEATPANAPILYQYGAFGKRLAKTDAVDEVFKNRRATVSLGYIGLYE
                     VATVFYGGEWETNPEAKDFTVDIIKKMKALVEDWSDEYGYHFSVYSTPSESLTDRFCR
                     MDTEKFGIVKDITDKEYYTNSFHYDVRKNPTPFEKLDFEKIYPAVGASGGFIHYCEYP
                     VLQQNPKALEAVWDYAYDRVGYLGTNTPIDHCYACDFEGDFEPTERGFKCPNCGNSDP
                     KSVDVVKRTCGYLGNPQARPMVKGRHKEISARVKHMNGSSL"
     gene            52030..52164
                     /locus_tag="ACHU66_000050"
     CDS             52030..52164
                     /locus_tag="ACHU66_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388371.1"
                     /translation="MGKYQLDDKGKALVTRYHEKNSNVKQDKKARVQELLKQAKNKKK
                     "
     gene            52166..53512
                     /locus_tag="ACHU66_000051"
     CDS             52166..53512
                     /locus_tag="ACHU66_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HFR3388372.1"
                     /translation="MDYTDISILHTNDMHSYMENFPKKAQLIANIRARNEKEGVPTFV
                     FDSGDLFSGNIFFNMYRGVKEIELMNQIGCQAMTLGNHEFDHGDELISRLDDYAQFPI
                     VSSNLRYQDVEVADELVPLEDVLEFDMNGKNLYVLGLTTLETQEVASPSENVIFEDHK
                     QALRRLVDQIVSQNPSAHLVLLSHLGYDEDVHLAKDFPELNVILGGHTHTILKQPSQI
                     GGVSICQAGQYGRFVGHLSLRCFADGRHEVQAYDLIEVEKLTQEDRAVKAIIDQMKSE
                     RDAAFSKPIAILPQALDGERDSIRQGLSTLAPVICQALFEQASQLGLQADGAVINGFG
                     IRASLSAGPIFYSDLVKVLPFSKRVLLVRIKGRDLLSSLQTGLHPQMWGIVQSGEDLL
                     VNGRAVESDKDYQIVTNSFVWSGKDNYDDFHKAEIVQDLGLDIDIISEFFKRQYGG"
     gene            53514..54008
                     /locus_tag="ACHU66_000052"
     CDS             53514..54008
                     /locus_tag="ACHU66_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR3388373.1"
                     /translation="MELRRPTIADKETILEMLAEFEAAGSAMDGGFISKDVDFEEWIF
                     RNKDYEAGLNLPDGFVPSIQYVSFDQTGRALGFLSLRLRLNDFLLNKGGHIGYSIRPT
                     ERGKGYAKDQLRLGLQEAATKNISKVLVTCSTDNQASRRTIVACGGVLEDVRDGVERY
                     WIED"
     gene            54195..54752
                     /gene="nrdG"
                     /locus_tag="ACHU66_000053"
     CDS             54195..54752
                     /gene="nrdG"
                     /locus_tag="ACHU66_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFR3388374.1"
                     /translation="MNNPKPQEWKSEELSQGRIMDYKAFNFVDGEGVRCSLYVSGCLF
                     HCEGCYNVATWSFKAGIPYTKELEDRILADLGQPYVQGLTLLGGEPFLNTGTVLPLVK
                     RIRKELPEKDIWSWTGYTWEEMMLETPDKLELLSYLDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLQEKRVVLWDKLKK"
     gene            complement(54857..54929)
                     /locus_tag="ACHU66_000054"
     tRNA            complement(54857..54929)
                     /locus_tag="ACHU66_000054"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54894..54896),aa:Lys,seq:ctt)
     gene            complement(54938..55131)
                     /gene="ssrS"
                     /locus_tag="ACHU66_000055"
     ncRNA           complement(54938..55131)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHU66_000055"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(55233..56519)
                     /locus_tag="ACHU66_000056"
     CDS             complement(55233..56519)
                     /locus_tag="ACHU66_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFR3388375.1"
                     /translation="MPANLALRMRPKSIDEVIGQEHLVGPGKIIRRMIDANMLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATTDNQKRLQEIAEEAKFSGGLVLMLDEIHR
                     LNKTKQDFLLPLLENGNIIMIGATTENPFFSILPAIRSRVQIFELQPLKTSHIRQALE
                     LALTDSERGFDFPVTIEPEALDFLANATNGDLRAAYNSLELAVLSTKESDDGSRHIDL
                     DAVENSLQKSYISMDKNGDAHYDILSALQKSIRGSDVNASLHYAARLIEAEDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALEAAQKIGFPEARILIANVVIDLALSPKSNSAYL
                     AMDAALADLRKNGHLPIPNHLRDGHYAGSKELGNAIGYQYPHAYPEKWVDQQYLPDKL
                     LAADYFTANDTGKYERALGMTQEKIKNLKKNRRQNP"
     gene            56593..57063
                     /locus_tag="ACHU66_000057"
     CDS             56593..57063
                     /locus_tag="ACHU66_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFR3388376.1"
                     /translation="MAKHGFLDVLEAALDKHFTYDYELNWDKKNHAVELAFILEAQNV
                     AGIETVDDEGNASSEDIFLEEYVLFYNQDKSRFDEEDYLVALPFDAKKGFSAEFLTYF
                     AGFLKDTADQGLDDLMDFLVDPEAQDFAISWDGEAFEKGRADLLEGEFYPYPRY"
     gene            57065..57418
                     /locus_tag="ACHU66_000058"
     CDS             57065..57418
                     /locus_tag="ACHU66_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFR3388377.1"
                     /translation="MVHEMLLAPKPFEMMKSGQKTIELRLYDEKRKHIQIGDRIRFYC
                     TENQTQTIEVQVLDLHIFDNFAQLYKELDLLSCGYTQSSIRGAKPEDMEDYYSREQLE
                     QYGAVGIELRVIDSI"
     gene            58014..58967
                     /gene="prmA"
                     /locus_tag="ACHU66_000059"
     CDS             58014..58967
                     /gene="prmA"
                     /locus_tag="ACHU66_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR3388378.1"
                     /translation="MNSWQELTIHVHRDAEEAVSNLMIETGSQGVAISDSADYVGQED
                     RFGELYPEVEQSDMIVITAYYPDTVDIEEVKADLATRLADLTGFGLETGQVSLESQEL
                     AEEDWADNWKKYYEPARISHDLTIVPSWTDYEATAGEKIIRLDPGMAFGTGTHPTTKM
                     SLFALSQVLRGGETVIDVGTGSGVLSIASSLLGAKEIYAYDLDEVAVRVAQENIDLNA
                     NTSNIHVAAGDLLRGVDIKAEVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIADK
                     WDMVRASAEAAGFFLETHMIQGEWNCCVFKKTADRSGVIGG"
     gene            58969..59715
                     /locus_tag="ACHU66_000060"
     CDS             58969..59715
                     /locus_tag="ACHU66_000060"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR3388379.1"
                     /translation="MQQYFINGRAPQGVFQISDKDTAKHMFSVMRLQADEQIVLVFDD
                     GIKRLARVVDSQNQSVEIIEELSDNVELPVSVTIAMGFPKGDKLEFVAQKATELGMAA
                     LWAFPADWSVVKWDGKKLGKKAEKLEKIAQGAAEQSKRNRIPAVRLFEKKVDFLAQLA
                     GFDQIILAYEEAAKEGEQANLVKILSGLEIGQSVLVIVGPEGGVSPEEVAAFEGAGAV
                     KTGLGPRILRAETAPLYALSAISYATELLR"
     gene            59729..60991
                     /locus_tag="ACHU66_000061"
     CDS             59729..60991
                     /locus_tag="ACHU66_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388380.1"
                     /translation="MSELEQKEHRELIVSDDCFALIQKMSELDSSAYQRYEITNPNIL
                     AHVEKIVQPALKHHLKKAGQPNIPKGLFKVDLPIGDLHKIKGETDKYRAFVKGAEGKF
                     KAHAILTPAQVEIAKGAKAAAAVSGAMQVAAMVVGQYYMAEINGQLDSMKETLNDVKD
                     FQVREFKGTVRALIVNISQLSKFSADYVENAELARLKLMECANFRAEVTKLLEQVNLA
                     IEESITKGKGLSFEKYEQLIDKLSTNLFYQQTLLRLLAELSKLDLVFSQGVASEDSSY
                     YVYEYYLNNCNSLGEKIANWHKDKIKFFQIDTKRNRRTQEGPMKVVADIAKELDKNAH
                     VAAGVGFLGGLVGAAAGFGIGKAAGELNKANRYIDVRKRTVKLISEQETVSYQSSKRL
                     SVNNYGENVKLLVEDGKVYYLLPDDSES"
     gene            61001..61716
                     /locus_tag="ACHU66_000062"
                     /pseudo
     CDS             61001..61716
                     /locus_tag="ACHU66_000062"
                     /inference="COORDINATES: protein motif:HMM:NF016365.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
     gene            61957..62514
                     /locus_tag="ACHU66_000063"
     CDS             61957..62514
                     /locus_tag="ACHU66_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388381.1"
                     /translation="MKRLSTPIFAALGFFLIVFVALNQFGRVPGTSYQIISELVLYNQ
                     ATEQTYEFENHEVRKAFIRQLSSTSSRGFQASDSRTSLETTYHETIMSENLISSIYGG
                     EEGRIMEFSRPRKSTLGNLVFASESVSYLVFARKYGNIRLETTILCQEFRVEQRLANQ
                     SNWVDLGTYTVKSPKETRYVPIYWE"
     gene            complement(62635..65124)
                     /locus_tag="ACHU66_000064"
     CDS             complement(62635..65124)
                     /locus_tag="ACHU66_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HFR3388382.1"
                     /translation="MKFRFSKCALTLSLALLASTNNALVSAEETSTDTPVLKTETEIS
                     SSVATSQAEATTGEVATPTTTETTAGEAVTPTTETTDQSVTPVTEEAAPVVETSNTPV
                     NDTTASPVTATRAAATETTTPVEGQSVDVRILATTDLHTNLVNYDYYQDKPVETLGLA
                     KTAVLIEEAKKENPNVVLVDNGDTIQGTPLGNYKSIVDPIEEGEQHPMYAALETLGFD
                     VGTLGNHEFNYGLAYLEKVIRTANMPLVNANVLDPTTKDFLYTPYTIVKKTFTDTEGK
                     KVTLNVGVTGIVPPQILNWDKAYLEGKVIVRDAVEAVRDIIPTMRENGADIVLVLSHS
                     GIGDDQYEVGEENVGYQIASLSGVDAVITGHSHAEFPGTAEKPSFYAKYSGVDDTNGK
                     INGTPVTMAGKYGDHLGVIDLNLVFKDGKWTTTSSKAAIRKIDTKSSVADGRIIDLAK
                     EAHNETINYVRQQVGETTAPINSFFALVQDDPSVQIVNNAQIWYAKQQLAGTSEANLP
                     ILSAAAPFKAGTRGDASAYTDIPAGPIAIKNVADLYLYDNVVAILKVNGAQLKEWLEM
                     SAGQFNQVDPSSTEPQNLVNTDFRTYNFDVIDGVTYQYDITQPNKYDRNGKVVNETAS
                     RVRNLQYNGQDVTADQEFIVVTNNYRANGTFPGVREASVNYLLNLENRQAIINYIIAE
                     KVINPTADNNWTFTDSIKGLDLRFLTADRAKELVANQEGLVYLQASAENEGFGEFKFV
                     YTEPKVATPDEQQSDQGNTGQDIVLESGQRITLPAVNPPAPAPQHKLASPHSQASTKT
                     LPATGEATSMLSLLGLTLIGFVGAWTKKKEH"
     gene            65388..67589
                     /locus_tag="ACHU66_000065"
     CDS             65388..67589
                     /locus_tag="ACHU66_000065"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR3388383.1"
                     /translation="MKEINFTGEEVVGLTATYLPAEDVAFVQKALDFATEAHKLQFRK
                     SGEPYIVHSIQVAGILAGLKLDAVTVACGFLHDVVEDTDVTLDDMEAEFGPEVRHIVG
                     GVTKLGKVEYKSHEEQLAENHRNMLIAMSQDMRVILVKLADRLHNMRTLKHLRKDKQE
                     RISRETMEIYAPLAHRLGISSIKWELEDMSFRYLNEVEFYKITRMMNEKRREREELVN
                     EVVDKLRDYTEERHLHGQIYGRPKHIYSIYRKMHDKKKRFDQLYDLIAIRCIMDTPSD
                     VYAMLGYIHELWKPMPGRFKDYIANQKANGYQSIHTTVYGPRGPIEFQIRTMEMHQVA
                     EYGVAAHWAYKRGGKATASEKELRWINNLIELQEGAGDAQTFVNSVKDDIFTERIYVF
                     TPDGAVRELPKDSVPIDFAYEIHTKVGERATGAKVNGRMVPLTTKLKTGDQVEIITSA
                     NSFGPSRDWVNLVKTHKARNKIKQFFKNQDKELSVSKGREMLQNLLQENGYVPNQYLD
                     RRHMDEVLQKTSYKTDESLFAAVGFGEVSAISIFNRLTEKERREAERAKAKAVADELV
                     NGGEVKHDNKDSLKIRHEGGVVIEGASGLLIRIAKCCNPVPGDEIVGYITKGRGVAVH
                     RVDCMNLKSQENYDVRLIDVDWEDENSTKEYMANIDIYGLNRSGLLNDVLKVLTNASK
                     NISSVNAQPTKDMKFATIHVSFGISNLATLTSLVDKIKSVPEVYSVKRTNG"
     gene            67591..68034
                     /gene="dtd"
                     /locus_tag="ACHU66_000066"
     CDS             67591..68034
                     /gene="dtd"
                     /locus_tag="ACHU66_000066"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR3388384.1"
                     /translation="MKIVLQRVSQASVTIEGSIHGQIEQGLLLLVGVGPDDTQEDLDY
                     AVRKIVNMRIFSDEAGKMNKSVQDVAGKILSISQFTLFSDTQKGNRPAFTGAAAPALA
                     SQLYDAFNQALSAFVPVEVGVFGADMAVSLVNDGPVTIVLDTKNK"
     gene            68110..68295
                     /locus_tag="ACHU66_000067"
     CDS             68110..68295
                     /locus_tag="ACHU66_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388385.1"
                     /translation="MKKLLTNHFFYLTIAFLLILVIYFSGIDKRWIILASFLYFIPSQ
                     ILYRRRLRERLQEDQPE"
     gene            68401..68664
                     /locus_tag="ACHU66_000068"
     CDS             68401..68664
                     /locus_tag="ACHU66_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388386.1"
                     /translation="MRKFFQDLNQEYLETRSSVGEDWLEWIIQRKMTWGMRWGLVGIL
                     MFVLQPLTYNPHFLLGCFYIGFLLWLVYVCGQLFLQPDLFSVI"
     gene            complement(68711..69370)
                     /locus_tag="ACHU66_000069"
     CDS             complement(68711..69370)
                     /locus_tag="ACHU66_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFR3388387.1"
                     /translation="MTPNKEDYLKCIYELGQLDQKITNKLIAEKMAFSAPAVSEMLKK
                     MVAEELISKDAKAGYLLSQTALEMVASLYRKHRLIEVFLVEQLGYSPEEVHEEAEILE
                     HTVSDHFINRLDLLLEQPQTCPHGGSIPQAGQPLIERYQTRLSQLTETGNYQLVRIHD
                     FYQLLQYLEQHELAVGDLLTVTAFDQFAQTITIQYKDKELAVPTAIAQQLFIEKSNRP
                     A"
     gene            69496..70419
                     /locus_tag="ACHU66_000070"
     CDS             69496..70419
                     /locus_tag="ACHU66_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFR3388388.1"
                     /translation="MKKILFSFALLLSLIGLGACRPSQTTEGSSKPRVAVTTSFLNDM
                     VYQLAGDEVERDLLIPAGEDPHLYVAKSSDLSKLQKADLVLYHGLHFEGKMVEALEKT
                     GVAVSKNFNAKDLNTMDEDGEEIVDPHFWFSIPLYKSAVAVASEELQKLLPAKAEMIQ
                     KNAEKYQAQLDDLHAWVEKELSVIPKESRYLVTPHDAFNYFAASYDFTLYAPQGVSTD
                     SEVANSDMIETVNLIIDHNIKAIFTESTTNPERMKKLQEAVKAKGGQVEVVTGEGKEL
                     FSDSLAPEGEEGDTFIDMYKHNVKLMVKYLK"
     gene            complement(70660..70929)
                     /locus_tag="ACHU66_000071"
     CDS             complement(70660..70929)
                     /locus_tag="ACHU66_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388389.1"
                     /translation="MKIKTRPTYQAEKVDKIKIPYQQIAGKPYTSLISTHQYMNLIFA
                     EHQCFLQSYVYKSVKNGPKTERTRPRPSPFYYSFEIPIHAQTTDI"
     gene            70973..71743
                     /locus_tag="ACHU66_000072"
     CDS             70973..71743
                     /locus_tag="ACHU66_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFR3388390.1"
                     /translation="MSAIIELKDVNLAYTASQTMALEDVNLVIPRGSRTAIVGPNGAG
                     KSSLFKVILGLEKPDTGQVRLLGQEAGLERLIAQKVAYIPQSSQVNWQFPATVFEIVL
                     MGRFAHSKGMFRRPTKADRQIVEQALERLKIADLRHRQIDQLSGGQRQRVFLARALAQ
                     EAELYLMDEPLAGIDQATEIMIMDMLKEFQCEGKTSIVIHHDLTTLDAYFDHLVWLHK
                     HVIDSGPMDETLTSENYQATYGIGNGLFLGNAKGGSHV"
     gene            71736..72596
                     /locus_tag="ACHU66_000073"
     CDS             71736..72596
                     /locus_tag="ACHU66_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFR3388391.1"
                     /translation="MFEVFKEYSFWTVALGTVSLAVAASTIGSISVLTKQSLLGDALG
                     HASYPGVIVSFMIFQSRHPLYLLLGAVLSGYLSYVLVHWLRRKGGHSLVNALSLVSAS
                     FFGLGMVLKNAIQGNEAFAGSSQAGLQTYLFGQAAFIQLDDVILIGIISLLALALFTF
                     FYQDYKLYLFDQTFARVIGVRVKYLQQLTMFLMICLIAVGLKLVGAILMSSFLIAPAV
                     FGLMLGKSYHKSLLLAGIVAIGSAFVGTWISSSVSGLSTGPTIIVCLTGLTLSAFVYV
                     TYVRKENGRV"
     gene            72589..73428
                     /locus_tag="ACHU66_000074"
     CDS             72589..73428
                     /locus_tag="ACHU66_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFR3388392.1"
                     /translation="MFEVLLILMVIASSCGLLGSILVVKNQSMLADALSHSVLLGIVL
                     GFFISHSLDSPLLIVGASLFGLLSVLAIDRLHSRKIAHDAATGLVFSFFFAVAVLLIS
                     LFARNVHLDVDLVLQGEVLFAPLHRMDVLAWSLPVSLVKSSLAWLVIVLFFAWAYHRL
                     QVYLFDSNHARLSGLRTRILEMVILILVSLTTVLAFEAIGSMTVIVFLVAPSMAALRW
                     VKSFWQLLLLGQGIAILTVVLGFLVANQLDLTMSGTCAVVSLLVVCSSIILKNTWSRS
                     SDK"
     gene            73484..75376
                     /locus_tag="ACHU66_000075"
     CDS             73484..75376
                     /locus_tag="ACHU66_000075"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFR3388393.1"
                     /translation="MTRLQDDFYEYVNGEWAKTAVIPDDKPRTGGFSDLADEIEKLMI
                     DTTNAWLAGEDVPEDSVLQNFVAFHKQVADYETRDRLGAEPAQALIAEYKALNSFEEF
                     TSKLAEYELAGKPNLMPFGVAPDFMDATTNVLWADSLGIILPDTTYYEEGHEKGAELL
                     KIWRESQEALLPKFGFSDEEIKDLLDKRLELDATIAKYVLSNEEGSEYAKLYHPYEWA
                     DFTALAPELPLDDFFTAILGQTPDKIIVPEERFWQAAKDIYSADNWELLKATLILKAA
                     GAYTAFLSDEIRILAGAYSRALSGTPQAQNQEKAAYNLAQGYFNQALGLWYAGEKFSP
                     EAKADVEAKVAKMIEVYKSRLETADWLAQETRDKAIVKLNVIKPYIGYPEALPERYYK
                     KIVDPSKSLVENAIELNKIDIAHGWSKWNKPVDIKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDSNGASFDEHGSLNNWWTEEDYAA
                     FEARTQQVIDQFEGQDSYGAKINGKLTVSENIADLGGIAAALEAAKSEDDFSAEEFFT
                     NFARIWRMKARPEFMQMLASVDVHAPGHLRTNIQLPNFDEFHETFGVQEGDGMWRAKE
                     DRVIIW"
     gene            75479..77980
                     /gene="leuS"
                     /locus_tag="ACHU66_000076"
     CDS             75479..77980
                     /gene="leuS"
                     /locus_tag="ACHU66_000076"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR3388394.1"
                     /translation="MSFYNHKEIEPKWQGFWAKNHTFKTGTDAAKPNFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPADFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKIKDTDKDFTVFTTRPDTLFGATYAVLAPEHDLVDSITSPEQ
                     TEAVAEYKRQASLKSDLARTDLAKDKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFGLDIIPVLEGGNVEEAPYTEDGAHINSDFLDGLNKEEA
                     IAKMVAWLEENGVGQEKISYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPENELPLV
                     LPKTSDIKPSGTGESPLANLTDWLEVVREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NDEKLADEDLLKAWLPVDIYIGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFNIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQFGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLLTTKELAAENSGALD
                     KVYNETVKTVTEHIEDLKFNTAIAQLMIFVNAANKEDKLYVEYAKGFVQLIAPFAPHL
                     AEELWQGLTNTGQSISYVAWPTYDESKLVESEVEIVVQIKGKVKARLTVAKDLAPAEL
                     EKVALADEKVQAEIAGQTVVKVITVPNKLVNIVTK"
     gene            78249..79610
                     /locus_tag="ACHU66_000077"
     CDS             78249..79610
                     /locus_tag="ACHU66_000077"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR3388395.1"
                     /translation="MLKTLKLVTIGGGSSYTPELVEGMILRAHQLPIREWWFVDVEQG
                     AKKQEIVVELARRMVAKAGLDWKIESTLDRREALIEADFVTSQFRVGQLPARYLDETI
                     PLSYGMLGQETNGAGGIFKAFRTIEAYKGIIKDMKDLCPNAWLINFTNPAGIVTEAIA
                     NELHWKKVIGLCNIPIGQKKLAAQILGQSEGDLSTRHIGLNHFHFHEVWDKYGENRTQ
                     VILETLYGDLREETPEAVKNITNLEFPIQLLRTIGMLPCDYHRYYFLEQEMLEDALEQ
                     YRQGNVRAKVVQEVETELFELYKDPQLKDKPKQLELRGGAYYSDVACQIICAIHNDSR
                     EELTVSTVNRGVIPYLPENCVVEVTSIISAQGATPLACPPTPPIVTGYLQLMKQMELV
                     TVQAAMTGDYSLALQAFTLNPLISNGPQAEVLLQDMLLAHENYLPQFAPAIQHIKERR
                     NNQ"
     gene            79607..81007
                     /locus_tag="ACHU66_000078"
     CDS             79607..81007
                     /locus_tag="ACHU66_000078"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFR3388396.1"
                     /translation="MTKDKVFYQFPTNFLWGSSTSGPQSEGIEPGDGKGQNNWDYWYS
                     IEPERFHNKIGPSQTSTFYKHYEKDIDLLVETGHTVFRTSIQWSRLIPTGTGEINQEA
                     VSFYRSVFSLVRAKGIQLFVNLYHFDLPYCLQEKGGWENKEIVRAYEAYARTCFELFD
                     DLVDRWITFNEPIVPVECGYLGNYHYPCKVDAKAAVAVAYHTQLASSLAVKACHEVNP
                     HKKIAIVLNLTPAYPRSEQAEDIKAARIAELFQTKSFLDPSVLGEYPDELVELIDAYG
                     LMPEVSDEELAIIKENRVDFLGVNYYQPLRVQAPTNSWKEGEPLTPEHFFAPYDMPGK
                     KMNPHRGWEIYEKGIYDIAINIKENYHNIEWFVTENGMGVEGEGAFKDNGLIQDDYRI
                     EFIEDHLIELHRAIQEGANCKGYMLWTFIDCWSWLNAYKNRYGLVELDLDTQERIVKK
                     SGKWFKKLSDANGFHR"
     gene            81017..82123
                     /locus_tag="ACHU66_000079"
     CDS             81017..82123
                     /locus_tag="ACHU66_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFR3388397.1"
                     /translation="MVELGFSIYPENYSLEESQAYIKLLHQYGARRLFMSLLQLENGD
                     RTVFELYQKLVDYANQLGMTVIADISPHFIAAQGWDGALMERAAELGLAGIRLDEALP
                     LEEIIEMTHNPHNIKVELNLSTDRTLLSNLLASVANRDNIIACHNFYPHAYTGLSQEH
                     FLEMSSFYYQSGIRTAAFVSAQTASEGPWPLSEGLPTLEDHRFWLLPLQIAWMKATGF
                     IDSILISNQFISEEELQSIQTVLNEDTISLMVDIEEGLTPVEREIVAFDHVYRGDISA
                     YVLRSTMPRVVYKDASVPARSDQAIPVGRGDILIDNDLYGRYKGELQIALKEFTISPK
                     VNKVGRISPDYLPLLRFIKPWQEFKLVIASEHFH"
     gene            82215..84209
                     /locus_tag="ACHU66_000080"
     CDS             82215..84209
                     /locus_tag="ACHU66_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFR3388398.1"
                     /translation="MLSKKEVRLLEALLAKQETFVPSIELAKVLSVSDRTVRTYIHQL
                     TSSLENSGAHILSKQGSGYCLQIERPVEFEMFWQEQLLLKKRLTDLTQLEEAGDRQRY
                     ILNKLLFEDPVQSPECLQRELFIGKTTLHSILADIRKLFIPYQLELLVTRKGIELKGD
                     EPAIRHFIMDYFFDEGNVQSVYGVVETNLLPDIRFTDLMLIVLDECRDAKLRLSDFVM
                     QNLVLHIALLLQRLKIGSGLDRFPIPERIQSSQEYLVAQRIIERIEEMFQVTIPSEEA
                     NYIALHLKVKLTGNPNQDGEVEEIFRQNVENSLLHLSHLTGLELSKDQQLFKGVLAHL
                     MPLRIRLENNIQLGNPLTAEIKDQYFEAFELTKTAFAAIPELMGYSISDDEWAYLTLH
                     VMAAIERYQGFKKTRVLVVCATGIGSALMLKNRLEKEFSSSLEILDTVSYYEVTEERL
                     KDVDLIISSISLSNLIFMTPVINVSVFLSEKDIEAIRSHLQQTTPVIPDQGESVLMEV
                     GQAKQIAEAVFAPHRIIYLEEDCDREVALKQLVTTLNESIEPDFVNRFIEQVNLRESY
                     SPVVFGDWLAFPHPALPMSYSEQIVVGIYKNAVDWGHGKQVQFVFLLSPSKGRNAFLK
                     YISPCLVDFVQDRVLQERLLHHPNYKELIDIFIPLIQRKG"
     gene            84222..84533
                     /locus_tag="ACHU66_000081"
     CDS             84222..84533
                     /locus_tag="ACHU66_000081"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR3388399.1"
                     /translation="MKHIMLVCNAGMSTSMLVTKMQKASEETGVEATIWAVPVSEADN
                     EVAQKEIDVLLLGPQVKFLLNDYKAKFEPTIKVDAINMMDYGVMNGAKVLEAALKMME
                     G"
     gene            84535..84849
                     /locus_tag="ACHU66_000082"
     CDS             84535..84849
                     /locus_tag="ACHU66_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFR3388400.1"
                     /translation="MNSPEYLEAIMGLIMYGGEAKGFASQAIQAAKEGRIDEAEQLLQ
                     DAIASLNIAHKSQTQLLAKEASGESVELSLLMVHGQDHLMNALTFIDLAKEFVDLYKQ
                     IG"
     gene            84868..85308
                     /locus_tag="ACHU66_000083"
     CDS             84868..85308
                     /locus_tag="ACHU66_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFR3388401.1"
                     /translation="MKISKIGQVPIERLFATIRQSLKDDFQQNTGSILQDQDIIQGLT
                     YTKTFGDKGQHSVKVTVEEFTAPYCYAVCFHSNRGKEHVRYQLEDLGNDQTNIEYSYE
                     MEAGDIFVKGNQFLMEKIFSKSIKRQTNAQLEALIRCSVEPVSV"
     gene            85352..86728
                     /locus_tag="ACHU66_000084"
     CDS             85352..86728
                     /locus_tag="ACHU66_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFR3388402.1"
                     /translation="MSNISEKFMEISGKIGSQRHLIAIRDSFISMMPITMAGTVAVLL
                     NVFLRDIPNNMGWTGFADAMQPVIDINGYVYFGTIGIMALVFAFTFGYNLSLMYKVNP
                     LAGGLISFASFISTIPQSVTISTTLDGVDKSVVSALEGLGLSIVTTDGVSALETSQWG
                     AIALKYGGATGLFTALFIGFLATWVYASLVKRNVIIKLPDSVPPAVNKAFAAIIPGTA
                     AIYASSIVAYLAFTLTGQSLGDLVSTYIQLPLLGLSQGLGSVILLTFLVQLFWFFGLH
                     GHNVLAPIMDGVYMVALNENAAAYTATHSTANLPWLWTRGSFDAYAQMGGSGVTLALI
                     IAIFIFSKREEYKMVAKLSAPMGVFNINEPVTFGIPMVLNPLFAIPWLIVPPICATIA
                     YLATAAGIIPPVFAPVPWITPPGLYAYLATGGNIMAALVSLFNLFVAFLIWTPFVIAA
                     NKVKAGEE"
     gene            87061..87669
                     /locus_tag="ACHU66_000085"
     CDS             87061..87669
                     /locus_tag="ACHU66_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFR3388403.1"
                     /translation="MSYQMIKKQFKLGLFTALIGNLLWLGMVNVSADDTVISDSPTVS
                     ALTKKVEAAKIAAKAAAEKIVAEKAAAAAKAALATNMVSEVAVEYTANTYPAGQCTWG
                     AKEMAPWVGNYWGNGGDWAASAAALGYEVGTTPKVGAIAVWTDGGYGHVAYVTDVAAN
                     GHIQVKESNYGGVYYPSNVRGFFDPTTTSEGTVSYIYPPAGV"
     gene            87821..88624
                     /locus_tag="ACHU66_000086"
     CDS             87821..88624
                     /locus_tag="ACHU66_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR3388404.1"
                     /translation="MKKIAMFTSLLFAGIVLAACNSSAGISNSTDQTLLAQIKEEGVI
                     QIGTEGAYAPYSYHDESGKLVGYDVEVAEAVAEKLGVKVEFVETEWDSMIAGLDAARF
                     DTIANQVGITDERKAKYDFSTPYTYIYGALVTQKDNTDITSFADLSGKKSANSLTSNW
                     ADLARENGAEVVGVDGFSQAVELLNTGRVDATINDNLVYLDYVKQHADAPIKVVTLTD
                     DVSTTAFPVAKGNEDLAKEIDAALAELASEGKLVEISNKYFGEDVSKAK"
     gene            88637..89296
                     /locus_tag="ACHU66_000087"
     CDS             88637..89296
                     /locus_tag="ACHU66_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR3388405.1"
                     /translation="MTERVWQLLLDSFSQILVPGLLVTIPLTILSFTFGLLIAIGTAL
                     VQIAQIPILKQVARFYIWVVRGTPLLVQLYVIFFGLPSLGIVLDAFPSAVLVFSVNTG
                     AYAAETIRASIESVPKGQLEAGYSVGMSFAQTMRRIILPQAFRVAFPPLSNTLIGLVK
                     DTSLAANITVLEMFMATQQIAARTYEPFALYCEVALVYLLFSTILTKLQAYGEKKLAV
                     Y"
     gene            89306..90052
                     /locus_tag="ACHU66_000088"
     CDS             89306..90052
                     /locus_tag="ACHU66_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR3388406.1"
                     /translation="MIQASGVKLTFGGQQVLKGIDVEVNPGEVVVILGPSGSGKTTLL
                     RSLNFLEKADAGHLVFGEDSYDLAQMTKKEIAGIRKRTAFVFQNYNLFANKTALQNVT
                     EGLIVARKVPKQEAERIGKAALDKVGLSGKYGFYPSQLSGGQQQRVGIARAIATNPEV
                     IYFDEPTSALDPELIGEVLAVMMQLAKEGMTMVVVTHEMGFARAVADRVIFMDGGRII
                     EEGRPEDIFDRPQEERTKQFLARIQKVDVE"
     gene            90417..91358
                     /locus_tag="ACHU66_000089"
     CDS             90417..91358
                     /locus_tag="ACHU66_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR3388407.1"
                     /translation="MTTLADVARLANVSKMTVSRVINHPEQVTQELRTLVTAAMEELN
                     YKPNVAAKALAQQRTLVVQAVILEKMDVVEPYYIDLLAGIADELNHRNYTLQLVTNPS
                     QITDQCDGYIVTGARKNDYPWLKGLRKPLILFGENQEGLPFVDSDNRLATRSATQFAL
                     SKGYERIVFVGIDLPEAFEKGREEGYKDAMQGRETHLYRLENRSRVAEEFVKNLDYLP
                     ENTCFICASDRLALGVTRGLQALGKSIPNDVGVIGFDGFFLDRMSRPALTTMKQPIRQ
                     MGALSVKHLMTILDGKPLARQGHYCQATLIERETTPQ"
     gene            91539..97844
                     /locus_tag="ACHU66_000090"
     CDS             91539..97844
                     /locus_tag="ACHU66_000090"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HFR3388408.1"
                     /translation="MKKKSVQNMKRTGMLEKKQIFGIRKLNVGVASVGIAAALFLSGA
                     GQLVQAEEVVLPASTSSTTLSEQDGGAVEATTTAIEATDSMVAEATAVTETADATAPE
                     EAPIEEGSIRLHFENVDETAPESQGLWTWGGVAEPSDSNQWPTDTANFSSSQVDDYGH
                     YVDIKKSETPGTIGYLVLKNGEKITESDQKVELLVPEQNEAWISSDYSVSSYEPLKDE
                     NVLRINYTREDNNYEGWGVWTWGDTTEASDGWPTGAVDFKLGKYGAYVDIPLSNGLDS
                     KLGFLLINQNNPDLAGNKTIDLAFADRKRHSQIFLRNGDDKVYTNPYFIEEKVELDTS
                     KATPGTKNVTVEASVKAPFNYNESGLVSVTVTNPENAEIVKMEVDTSAIGGGLVPIST
                     ELNRVTIKATSSTAPGTYSLPVKVYDKDNGYYETKLDVTITERVKADGEKDWDEQVIY
                     FMMTDRFYNGDTSNDKLVEGTVTNPRGLYQGGDFKGVTAKLDYLKELGVDSIWLTPIV
                     ENIPQNVGSVKDGEYYAYHGYWASNFEKLNPHLGSLADFHELIDAAAEKGINIVVDVV
                     LNHAGYGTEETFAGMVRTKEEDKQGDDQLGSLSGLPDFKTEEAAVRNQLVAWQASWLE
                     RSTTAKGNSIYAFRVDTVKHVDDTTWQHFKNELVDRDPDFHLIGETWGANYKDTKGDL
                     GIGTMDSLLDFGFKDIAKYLVNGQLKAAGKELEERSKVLTSAASLGQFLGSHDEDGFL
                     YSLGGAEKEGNLDKLKLAASLLITAKGQPVIYYGEEVGQSGQNNWPVYDNRYDFDWSK
                     VETSDITDHYQKLLAFRNANSTLLSRGDTSTLAGNDSQGWLISKRSYQDQAAYLVFST
                     NTESKEMALEVSSKDVVVTDAYTGKSYQAIEKGGKWVVQVELPTIGQGGTMLLQTEAG
                     DIVNASVQGATEEPIEAGYFRVHFKTLPSDNLSSLGLWTWDDVEKPSSDVGAWPTGAT
                     NFSTAKQDDYGYYLDIKMKDETASKISLLINNTSGDNITGDKTIERISTKMNEAWFDE
                     NYQLSLYQPLKEGCIRINYFRTDGNYDQKGLWIWGDVTDLTLGDWPNGIDFENQGKYG
                     AYIDVKLTDLPSSIGFLLLDESKSGDDVKIQQKDYSFKDLKNQTQIFLKDDDATIYTN
                     PYFVNNVRVTGVSHVSLTALEAAFTTLEGADKDSILEKLSVTDKNGQTVAVTDLVLDL
                     TSNKVRVLGDFNQENAGYTLKYGNDSFTTTMSWQLKDELYAYDGELGARVRQAGSVVD
                     MTLWSPSADSVAVVLYDKDDQSKVVGKLAMTKGDKGQWDLELNSQSGLGIADYRGYYY
                     HYEITRGGQSVLVLDPYAKSLAEWNSDLADTDPSYRIAKAAIVDPAEVGPSDLNYATI
                     PNFNQREDAIIYEAHVRDFTSDPAISDELTAQFGTFAAFAERLSYLKDLGVTHIQLLP
                     VMSYYFVNELKNAERMSEYASSNSNYNWGYDPQSYFAFTGMYSTDPTDPMKRIEEFKN
                     LVNEIHKQGMGVILDVVYNHTSKTFLFEDLEPNYYHFMEADGTAKSSFGGGRLGTTHY
                     MSRRVLVDSIKYLVDEFKVDGFRFDMMGDHDAEAIELAFTEAQKLNPNIIMLGEGWRT
                     FTGDANQPVQPADQDWMSSTDTVAVFSDEIRNTLKSGYPNEGQPAFITGGAKSVESVF
                     NNIKAQPGNFLADDPGDVIQYIAAHDNLTLFDIIAQSIKKDPSIAENYTEIHQRQRLG
                     NLLVLTAQGTPFIHSGQEYGRTKQFRHPDYKEPVSEDKVPNKAHLLTNADGTPFDYPY
                     YIHDSYDSSDAVNKFDWTKATDEALYPENTRTQAFTKGLIALRKSTDAFRLGTKEEVE
                     QKVSLISIPGQNGIAKNDVVIAYQTIASNGDRYAVFVNADSKERSFVLSDIYKELLKG
                     HVLVDGKRAGIAALTDLVGVTLTDDAVVLAPLTATVIRLPYITTAVPDTAPTAEEKPS
                     LDFTTKERTEEKILPIVEEIRYDASLSTDQSYVLQEGKTGKKVLVYQDVLIDGKVVAT
                     NLLSETVVDSETRIVVKGSMEAKKEAEKPSLSTTAQASGQATSGASKASLPITGDRQS
                     DLALLGLGLAGLGLTVAAQGRTKKSEE"
     gene            98192..99640
                     /locus_tag="ACHU66_000091"
     CDS             98192..99640
                     /locus_tag="ACHU66_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFR3388409.1"
                     /translation="MDFLQTPLNWFSQNILQNPAFFVGLLVLVGYALLKKKPHDVFAG
                     FIKATVGYMILNVAAGGLVTTFRPILAALNYKFQIDAAVIDPYFGLAAANGKIAEEFP
                     NFVSAATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATVSLMVLFLIPALR
                     NQFGTAAIGIICGIYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKIAPKLGK
                     KEENLDNLKLPSFLNIFHDTVVASATLMLVFFGVILFILGPEIMANPEVITSGAPYNP
                     AKQAFFMYVIQTAFTFSVYLFILMQGVRMFVAELTNAFQGISSKLLPGSFPAVDVAAS
                     YGFGSSNAVLSGFAFGLIGQLITIVLLIVFKSPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAAILSFISGVLQVALGAVCVGLLELSGGYHGNIDFEVPWLPFGFAFKYLGIAGY
                     VLVCLFLLAIPQLQFAKAKDKEAYYRGEAQAE"
     gene            99699..99977
                     /locus_tag="ACHU66_000092"
     CDS             99699..99977
                     /locus_tag="ACHU66_000092"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR3388410.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDFQSASCSVGEA
                     KGLAAGYDIVVASNHLISELEGRTKGHLVGLDNLMDDNEIKTKLQAVL"
     gene            100040..100525
                     /locus_tag="ACHU66_000093"
     CDS             100040..100525
                     /locus_tag="ACHU66_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR3388411.1"
                     /translation="MNLQKSFTENNSIRLGLTAETWQEAVHKAVEPLIDSGAATEEYY
                     DAIIASTEEYGPYYVLMPGMAMPHAQAGVGVLKDSFALITLTKPVVFSDGKEVSVLLT
                     LAATDPKIHTSVAIPQIIALFELENSIERLIACQTPEEVLAMVEESKTSPYLEGLDLE
                     S"
     gene            100536..101198
                     /locus_tag="ACHU66_000094"
     CDS             100536..101198
                     /locus_tag="ACHU66_000094"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014622185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR3388412.1"
                     /translation="MKHIPNLQVALDHSDLQGAIKAAVSVGHEVDVIEAGTVCLLQVG
                     SELVEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICSATIPTMAAALKA
                     IKEVRGDKGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNKV
                     KKLVDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAAAAREFKDEIRRI
                     WG"
     gene            101204..102067
                     /locus_tag="ACHU66_000095"
     CDS             101204..102067
                     /locus_tag="ACHU66_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFR3388413.1"
                     /translation="MARPIGIYEKATPKHFTWKERLEFAKELGFDFVEMSVDESDARL
                     ARLEWTKEERLDLVKAIYETGVRIPTICFSGHRRYPLGSNAPALEAKSLETMKQCIEL
                     AQDLGVRVIQLAGYDVYYEEKSPETRERFIKNLRQACDWAEQAQVMLAIEIMDDPFIN
                     SIEKYLAVEKEIDSPYLFVYPDTGNVSAWHNDIYSEFYLGHRSIAALHLKDTYAVTEN
                     SKGQFRDVPFGQGCVDWDNMFAVLKKTNYQGPFLIEMWSENCETVEETRAAIKEAQDF
                     LYPLIEKAGLN"
     gene            102067..102783
                     /locus_tag="ACHU66_000096"
     CDS             102067..102783
                     /locus_tag="ACHU66_000096"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR3388414.1"
                     /translation="MPKDLQEMRQRVYEANIALPAHGLVKFTWGNVSEVCRELGRIVI
                     KPSGVDYEKLSPENMVITDLDGNVVEGDLNPSSDLATHVALYKAWPNVHAVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTAEEVNSAYEKETGAVIIEEFERRGI
                     DPVAVPGIVVRNHGPFTWGKDPAQAVYHSVVLEEVARMNRYTEQINPRVEPAPSYIMD
                     KHYLRKHGPNAYYGQKGDEH"
     gene            102868..103485
                     /locus_tag="ACHU66_000097"
     CDS             102868..103485
                     /locus_tag="ACHU66_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388415.1"
                     /translation="MRKFLQDSLTETAYYLELILSAILGLALLALSTLLFADLLSSLA
                     GDIQADTFIQNFLSRAMTLAVGVEFIKMLYKQSPSTVIEVLLVAIARQLIVEHGSSSD
                     YLIGIISVAILFAVRKYLFTQFDDSNNIIMRASQKVKVANIIARVKIPSEGSETLRDF
                     VTRKLNEEEKTIAIGACVYLKNVALRIDNMHGELITRVEIIKSIY"
     gene            103610..105298
                     /locus_tag="ACHU66_000098"
     CDS             103610..105298
                     /locus_tag="ACHU66_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFR3388416.1"
                     /translation="MLLDKSSCALLRYLIGLQEPETIMAISKGLQQSRRKIYYHLEKI
                     NDALPTTVESIESLPRLGIRLTEAQKLACRDLLDSIDSYTYVMSMPERIQLMLVYICI
                     TPERVTIERLMELTEVSRNTVLNDLNEIRTQLAQEQYKMTLYVSKSHGYSLVCHPLNK
                     IQYVHSLLYTIFLDANKGFLQVLEDKLFELVGCQLLFSEEMAQFLQGQVSQLEKKLGK
                     KINRYEIEFMLKVLPFLLLSYRNMELDDLEERAILKEFEQVRERIEYRVAQELKDALL
                     QTFDLKLDEIEISLIAILLLSYRKDSDVHVFSQDFIDLKRVVERFIHHFEIHSPFELE
                     NPDELAKHLLAHCKALLFRKTYGILSKNPMVQQIQDKYSTLFAMTRVSGKILEEEWQI
                     ELSDGDIAYLTIHLGGAIRRSGTRKAQTQIVYLICDEGVSVQRLLYKQCQHHLPDKKI
                     GAVFTTEQFKSVEDLLEVDFLITTSDGLETDFPLVQVHPILDFDDVLNMTHFAKYRSL
                     ADEKQVFAVELDKLLSGYIADGRTARELKQRLQKLITNELLTNLSSQDIETDLY"
     gene            105408..106499
                     /gene="ulaG"
                     /locus_tag="ACHU66_000099"
     CDS             105408..106499
                     /gene="ulaG"
                     /locus_tag="ACHU66_000099"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR3388417.1"
                     /translation="MAKVQDITRESWILSTFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGVWIKTPGGANVVMDLWSSRGKSTKKVKDMVWGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHVHSDHMDISTAAAIINNPKLDHVKFVGPVESGDIWEKWGVPAD
                     RIIRIAPGDSFEFKDIKVHAVESFDRTCLVTLPIEGYDENGGKLAGLPVTDELMARKA
                     VNYVFETPGGTIYHGADSHFSNYFAKHGRDFNIDVAINNYGDNPIGIQDKMTSIDLLR
                     MAENLRAKVIIPVHYDIWSNFMASTDEILQLWKMRKERLQYDFHPFIWEVGGKYTYPM
                     DKDRIEYHHPRGFDDCFLEDSNIQFKALL"
     gene            106711..108681
                     /gene="tkt"
                     /locus_tag="ACHU66_000100"
     CDS             106711..108681
                     /gene="tkt"
                     /locus_tag="ACHU66_000100"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR3388418.1"
                     /translation="MSNLSVNALRFLGIDAIEKSKSGHPGVVMGAAPMAYSLFTKELR
                     INPAQPNWVNRDRFILSAGHGSMLLYGLLHLSGFEEVSMDEIKNFRQWGSKTPGHPEF
                     GHTAGVDATTGPLGQGISTATGFAQAERFLAAKYNRDGFPIFDHYTYVICGDGDLMEG
                     VSAEAASYAGLQKLEKLIVLYDSNDINLDGETKDSFTEDVRARYNAYGWHTDLVTDGT
                     DVDAIFAAIEKAKAAGKPSLIEIKTVIGHGSPNKQGTNAVHGAPLGAEETEATRKALD
                     WNYAPFEIPAEVYADFKANVADRGAAAYDAWVKLVEDYKVAHPELAAEVTAILEGRDV
                     VEIKPEDFPVYENGFSQATRNSSQDALNAAAKVLPTFLGGSADLAHSNMTYIKEDGLQ
                     DSVNPLNRNIQFGVREFAMGTILNGMAAHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPIEHLAGLRAMPNLNVFRPADARETQAAWYLSLTSKS
                     TPSALVLTRQNLTVEEGTDFDKVAKGAYVVYEVAGFDTILLASGSEVNLAVKAAKELE
                     AAGTKVRVVSVPSTELFDAQDAAYKEEILPNAIRRRLAIEMGATQSWYKYVGLDGKVL
                     GIDTFGASAPAQTVIDNYGFTVENVVKLVGEL"
     gene            108961..109482
                     /gene="tadA"
                     /locus_tag="ACHU66_000101"
     CDS             108961..109482
                     /gene="tadA"
                     /locus_tag="ACHU66_000101"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR3388419.1"
                     /translation="MNYTHEEKEYFMTQALQEARKSLEKDEIPIGCVIVKDGQIIGRG
                     HNAREELNQAIMHAEVMAIQEANNVEGNWRLLDSTLFVTIEPCVMCSGAIGLARIPQV
                     IYGATNKKFGAAGSLYDILTDERLNHRVKVETGILEAECANIMQDFFRQRRERQKAAK
                     LIMKTQEEATSDS"
     gene            109525..109614
                     /gene="ffs"
                     /locus_tag="ACHU66_000102"
     ncRNA           109525..109614
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHU66_000102"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            109754..110296
                     /locus_tag="ACHU66_000103"
     CDS             109754..110296
                     /locus_tag="ACHU66_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388420.1"
                     /translation="MFLFAFPGMGKTTLAKKYTDVVDLEMSDIKYDNSSVRHLTKEER
                     KSTKRTLKDKGYKTIYVNKAYTLHRQGKTVLVALNFLMRMLLAMSIRGGVPFHIYIPH
                     PSLKEEYRQRYISRGNNDRFIFEVMFIWYPALIPLYLLSKILPKWITVTQSGETLEDY
                     YKRENFVRFSQKLRFIQHIS"
     gene            110378..111727
                     /locus_tag="ACHU66_000104"
     CDS             110378..111727
                     /locus_tag="ACHU66_000104"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR3388421.1"
                     /translation="MTHITFDYSKVLGQFVGAQEVEYMQPQVTLADQMLRQGTGPGSD
                     FIGWLDLPENYDKEEFARIKEAAAKIQNESEVLVVIGIGGSYLGAKAAIDFLSNAFVN
                     LQTREERKAPQILYAGNSISSSYLADLVDYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKAKGAVKVEADANGWETFVVPDDVGGRFSVLTAVGLL
                     PIAAAGADIDALMEGANAARTTFTSDKIAENQAYQYAAVRNILYRKGYVTEILANYEP
                     SLQYFSEWWKQLAGESEGKDQKGINPTSANFSTDLHSLGQFIQEGNRNIFETVVRVDK
                     PRKNILIPDMAEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPQQDEF
                     TLGYTFYFFELAIAISGYLNAINPFDQPGVEAYKKNMFALLGKPGFEELGAELNARL"
     gene            111793..112521
                     /locus_tag="ACHU66_000105"
     CDS             111793..112521
                     /locus_tag="ACHU66_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR3388422.1"
                     /translation="MKKDFFVIGDVHGKFELLLDILKKWNEERQQLIFLGDLIDRGEN
                     SKACLELVWQLVREKDAICLTGNHERMFLAWLRDPLERYDHYRRNGGDTTINSLLGRP
                     LDAPVDGLVDANAVMEQYEKLIDFVKSCPYHLETEGYIFVHAGVDLTLPDWRKTSNHD
                     KVWLRTPFHEAENTTGKMIVFGHTPVYNLYQTKLRISQIWTSSDGKMGIDGGAVYGGV
                     LHGLVLNQQGLVRDYAVGCMESTQ"
     gene            112836..113369
                     /locus_tag="ACHU66_000106"
     CDS             112836..113369
                     /locus_tag="ACHU66_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388423.1"
                     /translation="MKKKWLLIFSALLTVTSLVACSNSKEETTSKSSSTEQTSTTKSS
                     TKESTSESSTVTLKESTVTYLADAEIDAIQTIGEYKTSLNSLMDSYVADFDGLIVQLP
                     EEAQTTLKPYREQIVTMLEQQKSALDSQFATLGDDSTVIPEEARATVLDSLKTVRDQL
                     KQTMTVVHEQAQTLLNE"
     gene            113526..114224
                     /gene="dapD"
                     /locus_tag="ACHU66_000107"
     CDS             113526..114224
                     /gene="dapD"
                     /locus_tag="ACHU66_000107"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFR3388424.1"
                     /translation="MTAQKMTAQEIIAFIGNAVKKTTVKVTFEGELAGPVPAEVTKLG
                     NVLFGDWKDVEPLLTNLTENVDYVVEQDGRNSAVPLLDKRNINARIEPGAIIRDQVTI
                     GDNAVIMMGAVINIGAEIGPGTMIDMGAILGGRATVGKNSHIGAGAVLAGVIEPASAE
                     PVRVGDNVLVGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEEALRTL"
     gene            114234..115361
                     /locus_tag="ACHU66_000108"
     CDS             114234..115361
                     /locus_tag="ACHU66_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HFR3388425.1"
                     /translation="MLDLIATRRALHQIPELGMEEFKTHAFLMETIENLLEDCSFAQV
                     RTWKTGILVYLTGSAPEKTIGWRADIDGLPIVEETELGFKSLHPGRMHACGHDFHMTI
                     ALGLLEKMAEQQPRNNLLFLFQPAEENLAGGMLMYEAGAFGDWLPDEFYGLHVRPDLK
                     VGQMATNRATLFAGTCEVKIRFTGKGGHAAFPHTANDALVAASYFVTQVQSVVSRNVD
                     PIEGAVVTFGSMHAGTTNNVIAETAFLHGTIRALTQNMSLLVQKRVREVAEGIALSFG
                     VDLEIELNPSGYLPVENNPQLADELMTYFDGVDGVEMIDCPPAMTGEDFGYLLNKVPG
                     VMFWLGVDTPYPLHNPRLSPKEEVLPFAVDKLSDFLKMKAN"
     gene            115403..115945
                     /locus_tag="ACHU66_000109"
     CDS             115403..115945
                     /locus_tag="ACHU66_000109"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR3388426.1"
                     /translation="MDKKEIRQEVLQRLKALTSSQRARWSQQLTERLLSSDTYKCSKS
                     LGTYLSMPHEFDTSYLIEQAQKDGKQIFIPKTYSQGRMDFVEYNPDDLMKSRFGVWEP
                     GTYSQPVDKSVVKLIHVPGLAWNQAGFRVGYGGGFYDRYLADYQGETVSTLADFQVYD
                     FEPDVFDIPVKELLIFEELF"
     gene            115929..116606
                     /locus_tag="ACHU66_000110"
     CDS             115929..116606
                     /locus_tag="ACHU66_000110"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFR3388427.1"
                     /translation="MKNFFDKRYPVTNALLAVTALVFLLIQIFRFGQTTTAYTIFEFG
                     GMYGQVVRYDPTQLWRLISPIFVHIGWEHFIFNSITLLGLGYQLEGLFGSRRFFLLYL
                     LSGIMGNLFVLFFTPDVVGAGASTSLFGLFAAMALLRKFSRSPYLQVLGQRYMVLLGL
                     NLVLGLFNPTISMAGHIGGAIGGCLVVIFLPPLVEKDLFTGKQIFFSFIGYMSLIVFL
                     LGIFYLI"
     gene            complement(116625..117524)
                     /gene="galU"
                     /locus_tag="ACHU66_000111"
     CDS             complement(116625..117524)
                     /gene="galU"
                     /locus_tag="ACHU66_000111"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR3388428.1"
                     /translation="MKKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALRSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKNDLLKLVDETTGIRLHFIR
                     QSHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDTKAVPLTKQLMNDYEKTHASTI
                     AVMPVPHEEVSAYGVIAPQGEGVNGLYSVETFVEKPKPEDAPSDLAIIGRYLLTPEIF
                     EILENQKPGAGNEIQLTDAIDTLNKTQRVFAREFKGNRYDVGDKFGFMKTSIDYALQH
                     PQVKDDLKQYLIDLGKKLDTKKN"
     gene            complement(117570..118586)
                     /locus_tag="ACHU66_000112"
     CDS             complement(117570..118586)
                     /locus_tag="ACHU66_000112"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR3388429.1"
                     /translation="MTKQTIAILGPGSWGTALGQVLNDNGHTVRIWGNVPEQIDEINK
                     EHTNKRYFKDVILDENIKGCKDLTEALDGVDAILFVVPTKVTRLVAKQVAQALKHKVV
                     VMHASKGLEPDSHKRLSEVLEEEIPAELRSEIVVVSGPSHAEETIVRDLTLISAASKD
                     LETASYVQNLFSNHYFRLYTNNDVIGVETAGALKNIIAVGAGALHGLGYGDNAKAAII
                     ARGLTEITRLGVAMGANPLTYSGLSGVGDLIVTGTSVHSRNWRAGDQLGRGEKLEDVE
                     RNMGMVIEGISTTKAAYELAQELDVYMPITQAIYKVIYQGAGIEDAIKEIMTGEFRHE
                     NEWH"
BASE COUNT        33813 a        22499 c        28118 g        34203 t
ORIGIN      
        1 gcacgcttgt actgaccgtg atgtaattaa ctctaataat attatatcac agcggagggc
       61 gctgacttgt gcaaacaact agcaaaaaga aaactaaagg agtttccccg ctggtgcagg
      121 gtggcagtcc tacatcatga ccaaatacag ataggtgatg attggactgt aaagctgttt
      181 gagtttgacc ctgaagacta caagggcaag gtacacggct ggcaacgtga ggcaccaaac
      241 gaggtcaacg agattttgaa agctatcaac acgatagcta aaccaagaca tcaggctata
      301 cttatcatga gttatatatt gcccgaaaag atacgatcag caaagcaggc acaacgactt
      361 gggatagctg aaagcactta ctacttggct aaaaatgaag ctttgaaaga gttcgccggt
      421 cagtaccgag atggctcact attgcagtac ttggatagtt aagtttgaaa tcattcgcaa
      481 atacccccaa cttttttaat cgggggtggg tttgttcgga gttctacaac gccgccctct
      541 tccgtgcaca attttccctt tttgaaattt tggatacagt aaaacacagt aggaaacagt
      601 agaagcatga tataatacac ttatcagcaa tcaaaaaagc gtaccgaatg gatacgctag
      661 tccttgcctg ctgaactcat tatttttagt ctatcatgct ataaatgata ggcttttttt
      721 gttccccttt ttgtacactt tctagggaaa tgtaacgctg tataaagttt acactttttt
      781 tcaaaaatcc agtaaaatca actaaaaagg cgtgtaaagc aaccaaatgc taaagccaag
      841 cataccttat cacaaaatgg tataatggaa ggtacaatgt ttgaaagagg aagacgatga
      901 aattacaaga gggagtagat cttcatttta ttgatacaga tcagtttacg acaaatcgta
      961 tacgtattcg ctttgcagct gaaatgagtg aggctacagt tgctggtcgt gtgttagttg
     1021 caaatatttt tgaaatgggt aaccaagaat ttcagactgc tcaggctgtt cggagaagat
     1081 tggcagaatt gtatggtgct cagttctcga cctcagtttc gaaacgtggt agggtgcact
     1141 gtgtagatgt gacaatttca tatgtcagtc ctcgtcactt accagaaaat gaggatatta
     1201 cagtggagat tcttgatttt ttatacacat gtatatttag accactgaaa aaggggcgag
     1261 gatttgatag ccagattttc gaggttgaaa aaacgaattt aatcaatttt cttcagtcag
     1321 agatagaaga taatttttat catgcagatg ttgaaatgag taagcttttt tataaagatc
     1381 cctctcttca aattccacgc gtcggtaggc ttgatttggt tgaaaaagaa acagcagaat
     1441 caacttttca gatttatcgg aatatgttgc gtatggataa aattgatata tttgtcttag
     1501 ggaaggttga cagagaacaa gtcaaaagaa aacttgaaga ttttggtttt acttatagaa
     1561 atccaaaatt agagttagaa tataatcagg aatactcaaa catcacgcaa gaaaaaatcg
     1621 agcgtaaaca ggcaaggcag tccattttgg aattggcata tcatttacaa gtagtttaca
     1681 acgatgtaaa ctatccggct ttgatggtat ttaatggtct actgggtgct ttctcccatt
     1741 cgaagttatt tatgaatgtt cgtgagaaag aaagtttggc ctatacaatt ggcagtcagg
     1801 tttctatttt ttcaggaatg ctgaaggtct atgctggaat tagccatgaa aacagactca
     1861 gggtaatgaa gttaattagt aaacaactac ttgatttaaa atgtggtaag tttacagaag
     1921 aagaattaga gttgacaaaa aacatgttga ttcattcagc aaccttggct caagataggc
     1981 agaataattt gatagaacaa gtatataatc aagttacctt aggaaataga aatttaagtt
     2041 ggttagattg gattgaggct atcaaatcgg tatcaataga ggatgtcatt cgagtaggac
     2101 agatgattaa tttacaggct gtttacttta tggagggaac agaagaatga agttagttga
     2161 gaagaagtat ccttacatga aagaatctgt cttttatggg aaagttgaca atgggttgac
     2221 agttattttg cttcctaaag tagatttcca tgagacatat ggaattctaa cgacaaattt
     2281 tgggggtatt catactcgat ttacattggc taatggtaaa agtgttgttt atcctgcagg
     2341 gattgcacat tttttagaac ataagctgtt tgaaactgag aatgaagagg atattatgaa
     2401 tgagtttgct aaattgggag caagcgcaaa tgcttatacc agttttagac agactagtta
     2461 cttattttca acgactcaga aagtattgga gtctctatca cttttacaat cttttgttcg
     2521 agaaccgtat tttacagaag acaatgtaga aagagaacag gggattattg aacaagaaat
     2581 tgagatgtat caagatgatg cagattatcg cttgtttacg ggaattttat catcgcttta
     2641 tccagaaagt cctttggcct atgatatagc agggacagtt gaatcaattg ctgcaattac
     2701 tgcggatgat ttacatgaga attttgaagt gttttatcat ccaagtaaca tgaatttatt
     2761 cgttattggt aattttgatt tagaggcagt ttggaaacaa atttcaagtt atcaagtggc
     2821 tcagatggat aatccagctc agtcgtttga attggcaggt gtccaaaagt tgccgataca
     2881 ggaacatctc tcagaacaat ttgaagtatc taccccaaaa ttggctgttg gtttacgggg
     2941 aaatgacgag gttgataagg agactattca gaaatatcgg cttagtttac aatttttatt
     3001 tgccatgttg tttggttgga cttcaaaacg atatcaacag ttatacgagc agggaaaaat
     3061 tgattcttct ttccagttcc aattagaggt tacacctgaa taccactatt tgattatctc
     3121 gggcgatact caagaaccca ttacgctttc tagtatactg atgaaagcac ttaggaaatt
     3181 cgaggatgat gcagatgtga cagaggatca tttacaattg ttgaaaaacg agatgtatgg
     3241 agattttatt cgaagcttaa attctcttga gtttacagct agtcactttg tttctcagta
     3301 ttcagaatat gaaaatgttt ttgatatacc tcaaatgata caaagtcttc agttagatga
     3361 catcagagag gcaggtcgtc gctttattga tcactgtgat atgacagatt tcacgatttt
     3421 tccaaaataa gtgggcgcaa cccgtcgaaa tttctttgga attttgttag aatagtatac
     3481 tgtacatgat gagcgataga gagaggatat tatgagacaa aagagtatag gggaagtact
     3541 tagaacagct agagaaggac ggagttggac atttgttgac ttgcaacgga taacaaaaat
     3601 ccaagctaaa tatttgcagg cacttgagta caatgatttt gattttattg caaactcaga
     3661 tgatgttcag tctattttaa aagcatatgc tgaggctttg gaattagatg cagatgttct
     3721 actggatgct tacgagacca atagtttagt gaaatattat gaagatggag aagaagaact
     3781 acgctcttca gaattgaaaa gaagctataa ggttcgtaaa cgtaaaaaga actcttacct
     3841 tccgttgatt tacttattac ttgcgacggg tttgattatt atttttgtga cttatattgt
     3901 tcatagcagg ctccaaaata gagcatctat tacgcctact acaacgtcat atagtatagt
     3961 tgagcaatct gtatctgaag caagtagtgg cacagagacg agttctgctg agcaggcgca
     4021 gtcaaattca acgagttcaa gtagttccgt tacagaaaat gtgactattt caggatcagg
     4081 ttctaacatt gttgcgaccc ttaaaacagt gacttatcca gtagaagtaa ctgtaacagc
     4141 aaaaaataca acgagttgga tcagcctttc tgacaccttg ttagaggggg gcgttacact
     4201 aacacctgat aatccaacag taactactac tgttgaagaa ggagtgactt ctactacact
     4261 tattcttggt gttgtcaaag gagtagaggt aactgtaggt ggccaaaagc taaatctatc
     4321 agctctgaca gcagatacag gctcaattac aataaatttt gaacaataaa ggttttatat
     4381 gaaaaaagaa aatattccca atgcattgac gataggtagg attttggtaa tcccaatttt
     4441 tatcttgtta ctgaccgttt gggattcaac aattagtcat gtcttagcag cacttatttt
     4501 tgcattagct agtattacgg attatctaga cggctacttg gctcgtaaat ggcaggttgt
     4561 aacaaacttt ggaaaatttg cggatccgat ggcagataag attttggtga tgacagcatt
     4621 tatcatgctt gtagagcttg gctttgctcc agcttgggta gttgcaatta ttatttgtcg
     4681 tgaattggca gtgacaggtc ttcgtctact cttggtggaa aatggaggaa cagttctagc
     4741 tgcggctatg ccaggaaaaa tcaaaacatt ttcacagatg tttgcagtta ttttcctgct
     4801 cttacactgg aacgccgtgg ggcaaataac actctatatt gcacttttct ttaccctata
     4861 ttcaggttat gattatttca agggtgcgtc cttcttgttt aaagatacat ttaaatgatg
     4921 aatattattg aagtaaaaaa tttaaaatat aaatacaacc aagaggatga gcgctatact
     4981 ctaaatgatg tttcgtttca cgtgaaacag ggagagtggc tgtctattat tggtcataat
     5041 ggttcgggta aatcaacaac agttcgtttg attgatggtt tgttggaagc tgaatcaggt
     5101 gatatttata ttgacggtga tgccttaacc attgataatg tatgggataa gcgacgcttg
     5161 attggtatgg tttttcaaaa tccagataac caatttgtag gcgctacagt tgaagatgat
     5221 gttgcatttg gtttagagaa tcagggaatc cctttggagg agatgcgtag tcgagttgac
     5281 gaagctttgg aattagttgg aatgactgat ttcaaaacac gtgagcctgc tcgtttatct
     5341 ggtggtcaga agcagagggt ggcgattgct ggagtggttg ccttgcgtcc aaaaattatt
     5401 attttggatg aggctacgtc tatgttggat ccagaaggtc gtctcgactt gattaagatt
     5461 gtccgtgaaa ttaaggagcg tcatggtatg acggttattt ctataacaca tgatttggat
     5521 gaggttgcat tgagtgatcg agttatcgtg atgaaaaatg gacaggttga atcaattagc
     5581 actccaaatg aactatttat gcgagaagat ttggtagatt tagacttgga tagaccgttt
     5641 acgacagaac tggcttcatc tttgcgccag actggacttg atttaccgct ccgctatttt
     5701 acagaggaag aattagagga aactttatgg gaattgatct ccaagaagta agctatacct
     5761 atcaagctgg cactcctttt gaggggcgtg cgctttttgg tgtggatttg gagattgttg
     5821 atggctctta cacggccctt attgggcata cgggttcggg caaatcaacg attttacaac
     5881 tcttgaatgg attaaatgta ccaacagaag gaagagtagt cattgatgat gttgtgatta
     5941 cagcgacgtc tgaaaataag gatattaagc aagtcaggaa gaaagtgggg ctggtgttcc
     6001 aatttcctga aagtcaagtg tttgatgaaa cggtcttaaa agacgttgct tttggtccac
     6061 aaaatttcgg agtttctcag gaagaggctg aaacgattgc gcgtgaaaag ttggccttgg
     6121 ttggaattga tgaaagcttg tttgagcgta gtccttttga actatctggt ggtcaaatgc
     6181 gtcgggtagc tattgctggg attctggcaa tggaaccaag tatcttagtt ttggatgaac
     6241 caacggctgg attggatcct gcgggtcgtc aggaattgat ggatattttc aaaaagcttc
     6301 atgaatcagg catgacaatc gttttggtaa ctcatttgat ggacgatgtg gctaactatg
     6361 ctgattttgt ttacattatg gaaaagggaa agctggtccg ttctggtaaa ccagtcgata
     6421 tttttcaaga ggtagatttt ttagagagta ttcagttggg agtgcctaag attactaagt
     6481 ttgcagctaa tttggagaga agaggttttg tacttgaacg tcttcctatt acaattgaag
     6541 aatttacggg gatggtgaca catggataaa ttaattttag gtcgctatat tcctggggat
     6601 tcaattattc atcgtttgga tccaagaagt aagcttttag caatgtttgg atttctattg
     6661 atgatttttt gggccaataa tttggtcacc aatgccttgt tgatagcatt tgttttagga
     6721 atggttgttt tatctcgtat tcgcttatca ttttttatta atggtttgaa accgatgata
     6781 ggaattattc tatttacaac tttcttccag gttttcttta cacctggagc gaccattcta
     6841 tgggaatttt ggatttttaa attatctgta gaagggttgc aacaggcggc gattattttt
     6901 gttcgctttg ttttgattat tttcttctca accctattga ccttgacgac gacaccgctc
     6961 agtttggctg atggtattga gtcggggctt gcacctttga agaaatttaa agttcctgtg
     7021 catgaaattg gtttgatgtt gtccatgagc ttgcgttttg taccgacctt gatggatgat
     7081 accactcgga ttatgaatgc tcagagagct cgtggagtgg actttaatga gggaaatctt
     7141 attcagaagg taaagtcagt tattcctatt ttgattccgt tattcgcatc aagttttaag
     7201 agggcagatg ctcttgcaac ggcgatggag gcgcgtgggt atcaaggcgg agatgggaga
     7261 accaagtacc ggattttgga atggaagtta gcagatacgt tagcaatttt agttatgtta
     7321 gctttagctg gactcttatt tatattgaaa aaataagtaa aaaacgctag aaatagcgtt
     7381 tttattgtct aatctcaatt tgtctgtaat atttgtaaca atttcacaaa gtctaagtaa
     7441 tagttagagg gtatgataga cacatgacag aaaggaatgt gactattatg aacattacat
     7501 taaagaaaaa cttaaaaaca actattgctg gtttggtagc gggaatctcc cttttaactg
     7561 cgggtgtagt cagtgcagaa acctatactg taaagtctgg tgataccctg tctgaaattg
     7621 ctgaaacata caatactact gttgaaaaat tagcagagca gaataagatt accaacttag
     7681 attttatcca tgtcggtcaa gttatcgaat tgggagatgt gacagtgaag gcagtagaga
     7741 gttcaattga acaaactcaa tcaacatcaa cttctactgc aacagtaaca actagcacct
     7801 attcttcaaa tttgagtgct gaagatgcag cagcaaaaga atggattgca atgaaggaat
     7861 caagcggtag ctatgatgcc cgtaacggta tctacattgg tcgttaccag ttgacaaata
     7921 cttacttgaa tggtgactac tcaccagaaa accaagagcg tgtagcagat gcttatgttg
     7981 ctagtcgcta tggttcatgg tctgcagcca aggctttctg gttggcaaat ggttggtact
     8041 aagataaatt agaagaggtc tagcacgatg ctagactttt tctgtctcac gaatgtattt
     8101 cgcaactgct ttttcgttca gtccaacggt actgaatagt aacctctagc ccaaaacttt
     8161 tgatttccac gctgtatgga cataggtgat ggaaaatatc aattaagtct agtctaattt
     8221 tttagtaaat ggattttctg cggtacttag gagtgacata catttggtat gtgataaact
     8281 gtaacggttt taaccatgct aagctagtcc actggactag tttactcctc ctttatttaa
     8341 cttgacaaat tagattataa caggaaaaaa atagaagctc aaagccttgc cagccaccag
     8401 tataggtggt atttttcttg tttttctcta tgagaaaact agcccgaagc cataataaga
     8461 aaaaggaccg ttagggtcct ttttggttta cattattgtc aatcttcccc aaaaatttct
     8521 tgagtaaggc gacgacccgt cggtgtagct gcaaggccac cttcagcagt ttcacggaag
     8581 gcagttggga gggcggctcc aacttggtac atggcattga taacttcatc cacagggatt
     8641 tttgattcaa ttcctgccag tgccatatca gcagccacca tagcgtagct agctcccatg
     8701 gcgtttcgtt tgacacatgg gacttctacc agaccagcga cgggatcgca aatcagaccc
     8761 agcatatttt ttatgacaaa gcagatggct tggctggctt ggtagggagt tccacctgct
     8821 gccagtgtca gggctgcggc agacatagca gaggcagatc cgacttctgc ctgacagcct
     8881 ccttctgcac ctgagatgga ggcgttgttg gcgataacta gtccaaaggc tccagctgta
     8941 aataggaagt caagttgctg ttcttctgtc aaattgagct tttcaatagc aactcctagc
     9001 acagctggta gacagcctgc agatcctgct gtcggagtgg cacagactaa gcccattttg
     9061 gcattcaatt cattaacggc catggcattt cttgcggcgg ataggacagc ggaatctgac
     9121 agggttttac cagatttcat ataggcatct aacttggcag catcgcctcc tgttaaacca
     9181 gatacagatt tactttcagt aagtccatct acgatggagg ctttcataac tgtcaagttt
     9241 cttgacataa ttgtcaatat ttcttcgcgg ctacgaccgg tcaactcaat ttcagtcgcg
     9301 atcatgagtt cagctacatt agcagaaaag ttgtctgctt gctgaactaa ttcttcgata
     9361 gtataaaaca tgtctttctc tttctagttg aagaaggtta cattgtgcag gtgtggaatt
     9421 ttttcaattt ctgcaataga attttcacat tggcgagcat ccacttcgat aatcataatg
     9481 gctttttcgc cagctttttc acgtgtcacg ttcatttggg cgatattgat gtcatactta
     9541 gaaaggacat cggtcacttt cgcaatcata ccagggacat cttggtgaac aataataata
     9601 gttggcgtat tcatattgag attgacagag aagccgttga gctctgttac ttgaatgttt
     9661 cccccaccga tggaaacgcc tgtggctgag atggattttt tatcgttttt tatgatgatt
     9721 ctggtggtat tgggatgtgg ggtattgctg tccttgttga cacgccagta aaccttaatg
     9781 cctcgttctc ttgcaatatc aagagagtca ggaatgcggg gatcatccgt atccatgccc
     9841 agaatacctg ctaccaaggc tacatccgta ccgtgaccac gataggtctt ggcaaaggaa
     9901 ttgtagagtt gaaattccac ctctgtcggc tcatcgccga agatggaaga tacaattttc
     9961 ccaattcgga ccgccccagc tgtgtgacta gaagatggac cgatcataac aggtccaata
    10021 atatcaaaga ccgactgaaa ttttaagttt gtcatagtaa tcctcacgtt tttcttggta
    10081 ctattatatc aaattttcta gttagagaat atgcctagat gaatgtgttt tcttatgtta
    10141 aaattctatc gcttaccctt tttgtatggt ataatacaag ttgttcaaaa tcgtcaatcg
    10201 tttttggata atcaaatttt ggaatacgac cttcaaatcg ttatttctac gaaaagatgg
    10261 gagaagatta tgtcaattga caattctaaa acccgtgttg ttgtcggtat gagtggcggt
    10321 gtggattcat cggttacggc tctcttgctc aaagagcagg gctacgatgt gatcggcatc
    10381 ttcatgaaaa actgggacga cacggatgaa aatggcttct gtacagcaac agaagattac
    10441 aaggatgtgg ctgctgttgc agaccagatc ggcattcctt attactctgt caactttgaa
    10501 aaagagtatt gggatcgcgt atttgagtac tttttagcag agtatcgggc aggtcgcaca
    10561 cccaatccag atgtcatgtg taacaaggaa atcaagttca aggctttctt ggattacgct
    10621 atgaacctgg gtgcggacta tgtggcgaca gggcactacg ctcaggtgtc acgcgacgag
    10681 gacggcaccg ttcatatgct gcgtggggca gataatggta aggaccagac ctacttcctc
    10741 agccaactct cacaggaaca gctgcagaaa accatgtttc cgctcggcca tttacaaaag
    10801 cctcaggtga gggaaatagc agagcgagca ggcttggcga cagctaaaaa gaaagactcg
    10861 acaggcatct gctttatcgg tgaaaagaac ttcaaagaat ttctagggca gtatttgcca
    10921 gcccagcccg gtcggatgat gaccgttgat ggtcgtgata tgggtgaaca cacaggcctt
    10981 atgtactata ccattggcca gcggggcggg cttggtatcg gaggacaaat cggtggggac
    11041 aatgagcctt ggtttgttgt cggaaaagac ctgtcaaaaa atatcctcta tgtcggtcag
    11101 ggcttctatc atgagtcctt gatgtcaact tctttacagg ctagtcaagt ccactttaca
    11161 cgtgatatgc ctgaagaatt caagctggaa tgcacagcta agttccgcta ccgtcaacca
    11221 gacagtcaag taactgtcaa ggtgaaaggt gacaaggcag aagtcatttt tgcagagcca
    11281 caacgagcaa tcacaccagg acaagccgtt gttttctacg acggacaaga gtgtctgggc
    11341 ggtggtatga ttgatatggc ctataaagat ggagaggctt gtcagtatat ttaatcaaaa
    11401 ataaaagctt agcacccttg tgctgagctt tttatcttat ccccagaaag catcttcggc
    11461 ggtttggtcg gctgagaaga gccaaacttc cttgattttg ccatcttcca tacggaaaag
    11521 atcgatgcca ttcatgttta gttcttcacc gtctgttcga gtaccgagga atgttacgtt
    11581 agcggcaaca agagtatcat tatcggatac ccagtttgtc actactttga atgttccatt
    11641 tgttttttcg gcgaaagttg ctagatgtgc tccgagtact tccttaccaa ttttagcacc
    11701 agatgttgaa tgatttccgg gttgatgcca gataatgtcg tctgccattg tttcaaaaag
    11761 agctgcaaaa tcaccagcga tgagagcatt gttataggca ttaaaaattt ccaagtttgt
    11821 tgacatttga tgtcctccaa tttttttgat atagtacaat tatagtggaa gtaatcaatt
    11881 atacaagtag gtacttttgc atttgttggt atcaaaagtg atactattgt gataaataaa
    11941 ggagaatttt tttgaaaaaa gttagtgaat atggtatttg cccttttgca acaacacaaa
    12001 aggtcttgac tggtaagtgg ggattggtga ttatctacca actcagtaca ggaacgaaac
    12061 gcttcaatga attacagcga ctgattccag gtatcactca aaccatgtta acaaggcacc
    12121 ttagacaatt ggaggaggat cgcattatta gtcgcacggt ctatgcagaa gtcccccctc
    12181 gtgtagagta tagcttgact gaaatgggcg aaaaatttcg ttcggtgctg gatgctgttg
    12241 aggcttgggg cttagaatat attgaaacgt taacgtaaca ccagatttga aattctacaa
    12301 atcttttgct aaaataagag taacaatcat catgatggtc aaagctcatg ggaattgtag
    12361 gcttttttgt cctgcaattt tcgagagttt tggctatttt ttgctattgg ggagagaaga
    12421 tggatttcag aaccaaagtt gacaaccaga ttttcggtgt ccgtgccact gcattgctta
    12481 taaaagacgg gaaaatattt ctcactaaag actctaaggg gcgttattat accattggcg
    12541 gtgcggttga ggtcaatgag gtagcagcag atgcggttgt tcgagaagtc aaagaagaat
    12601 tagggattga cagtcatgtc aaccaactgg cttttgttgt cgaaaataag tttacacagg
    12661 aaggcataca ctttcacaac atcgagtttc attttatcgt ggagcctatt ggagaaatac
    12721 ctgaggagat gattgaagat aacttgaaac aagcttgtga atggatagac gttgacaaac
    12781 ttgtcaacct agatgtcgta ccagtttttc tggcagagaa attgccaaac tggagtggac
    12841 aagttacaca cattatgaac atgaaggaga aaacaacatg acacacacat ttgcagaaaa
    12901 ttatgatgtc atcgtgattg gtgcggggca tgcgggtgta gaagcaggat tggcagccag
    12961 tcggatgggc tgtaagacct tgcttgcaac tattaacttg gatatggtgg cctttatgcc
    13021 ttgtaatccc tccattggtg gctctgccaa ggggattgtg gtacgagaaa ttgatgcctt
    13081 gggtggcgaa atgggtcgca acattgacaa gacctatatc cagatgaaaa tgcttaatat
    13141 gggtaaaggg ccagctgttc gtgccttgcg ggctcaggca gataaggcag agtacgcagc
    13201 agagatgaag cggacggtgg agcgtcagga aaatctgacc ctgcgtcaaa ccatgattga
    13261 tgagatttta gtggaagacg gcaaggttat cggtgtccgc acggctacca accagaaatt
    13321 ttcagccaag gcggttgtgg tgacgacggg tacagccttg cgtggtgaga ttatcatcgg
    13381 tgacctcaag tattcatcag gacctaacaa tagtctggct tctatcacgc tggcggataa
    13441 tctgaaagaa ttgggacttg aaatcggtcg tttcaagaca ggaacaccac cgcgtgtcaa
    13501 tgctcgtacc attaactacg aagaaaccga gattcagcca gacgatgaaa agccaaatca
    13561 cttttcattc ttgtccaagg acgaggatta tttgcaggat cagattcctt gctggttgac
    13621 ctatactaac gcgaccagcc atgagattat caacagcaat cttcaccgag cacccatgtt
    13681 ttcgggcatt gtcaagggga ttggtccccg ttattgccct tctattgagg acaagattgt
    13741 ccgttttgcc gacaaggaac gccaccagct tttcctagag ccagaaggtc gcaatacaga
    13801 cgaaatctat gtccaagggc tgtcaaccag tctgccagaa gatgtgcagc aggacttgat
    13861 ccactccatt aaaggtttgg aaaatgccca aatgatgcgg acaggctatg ccattgagta
    13921 cgatatggtt atgcctcatc agttgcgggc aacgcttgaa accaagaaga tttcagggct
    13981 ctttacagca ggtcagacca acggaacatc aggttatgaa gaagcagcag gtcagggaat
    14041 tgtcgctggt atcaatgcag ccctcaaggt gcagggcaag cctgagctga ttttgaaacg
    14101 aagcgacggt tatatcggcg ttatgattga tgacctggta accaagggaa cggtggagcc
    14161 ttaccgcctc ttgaccagtc gggcagaata ccgcttgatt ctgcgtcatg acaatgctga
    14221 tatgcgactg acggagattg gtcgtcaggt tggcttggta gatgatgaac gctggcaggt
    14281 cttccaaatc cataaaaacc agtttgacaa tgaaatgaag cgcttggagt ctatcaaact
    14341 caaaccaatc aaggaaacca atgaaaaggt tgttgccatg ggcttcaagc ctttgacaga
    14401 cgctctgact gccaaggaat tcatgcgtcg tccagatgtg acttacgcag atgcggtagc
    14461 ctttatcggt cctgcggcag aggatttgga cgccaagacc attgaattga ttgaaacaga
    14521 ggtcaagtac gagggctaca ttgccaaggc cttggatcag gtggagaaga tgaaacgtat
    14581 ggaagaaaaa cgcattccag ccgacattga ctgggatgat attgactcca ttgcgaccga
    14641 ggctcgccag aaattcaagc tgatttcacc agaaaccatt ggtcaggcta gtcggatttc
    14701 cggtgtcaat ccagcggata tatccattct catggtttac ttggaaggac gtagtcgttc
    14761 catttctaaa aacaaatcaa aggacagtca ctaaggctgt tcttttcgct gtatgtcagc
    14821 tatatgtcaa ctactttaaa atcatgtgaa aagtagcttt tttactgaat ttatggtaga
    14881 atggcaggca gaggttagtg atgaaaagat ttcgattttc gacaattcac tttgtcatga
    14941 taggtgtcat tttatttggg ttggtggctc ttgtccaccg attttttcca attggtgcca
    15001 gtacagtttt tgcgctactc atcagcctat tggtgttgat tgcgctgttt atttatcaaa
    15061 aacattctta tgaatttagt gagctagaac agattgaata tctcaataat caggcaaatt
    15121 ctggtttgat gatgctcttg gataaaatgc cagtgggagt tatcaaagtt cgaccagata
    15181 gtaatcaggt agaatggttt aatccctttg ctgagctgat ttttgctcaa gaaaatggtg
    15241 agtttgatca aaaaaaacta cgtgaaatta ttgacgttgg tctggatgag gagcgaattt
    15301 atgcaaactt ttcaggcaag agatacgctg ttaacgttga ttttgatcaa gggttatttt
    15361 acttttttga tgcgtcgaca gagtattcag cgacaaatag tttgatcgga agccgtcctg
    15421 ttatagggat tatttcagtt gataactatg atgagcttga ggatgctgta actgattctc
    15481 aaattagcca agtcaattcc ttcttggctc aatttgtttc ggaattttgc catgattacg
    15541 gtatctacta ccgacgcggc acaatggatc gattctattt ctttacagat tatgctgtgt
    15601 tggaaaaatt gatagagaat aagttttcag ttattgataa gttccgcgag gaggctaaaa
    15661 agttagattt aggtttgact ttgagtatgg gattggcgta tggtaatgaa aatcatcacc
    15721 aaatcggaca ggttgcgctc caaaatctca atatggctga ggttcgtggt ggagaccagg
    15781 ttgtggtcaa ggaaaatgat gagagtaaac agccactttt ctttggcggt ggctcggctt
    15841 cttccgttaa acgaacacgt acgcggactc gtgctatgat gactgctgta tcagataaac
    15901 tcaaatcggt tgatgctgtt tttgtggtgg gacatcgtaa tttagatatg gacgcgcttg
    15961 gatccgcggt cggcatgcag tactttgctc aaaatattat gaacaatgcc tatacggtgt
    16021 ataatccaga tgaaatggca ccagatattg cacgtgccat caagaagttg agtgatgaaa
    16081 attgttctaa tttgctaacg gttgaggaag ccatgaagat ggtcactttc cagtcgttgt
    16141 taatcatggt agatcattca aagattggct tgactttgga taaggatttc tatgaccaat
    16201 ttacccaggt tgtggtggtt gatcatcatc gtcgcgatac agatttccca aataatgcag
    16261 ttttgactta tatagagagt ggtgctagtt ctgcgagtga gttagtaacg gaattaattc
    16321 agttccagaa tgacaaacac cataagctta atcgcattca atctagttta ttgatggctg
    16381 ggattatgct tgatacaaaa aatttcacct ctcgtgtgac tagtcgtact tttgatgtgg
    16441 ctagttactt gcggactcgt ggtagcgata gcttggaaat ccagcagatt gctgcgacag
    16501 attttgagga atatcgtcaa atcaatgaac tgattttaag cgggcagaga attgaggcca
    16561 atgttgttat tgcgtgtgca gatgatacta aagaatacga taatattatt ccgagtaagg
    16621 ctgccaatac aatattggac atggcaggaa ttgaggcggt attcgttgta acaagaaata
    16681 ccaaagggta tgtatctatt tctgcaagga gtcatagtaa aatcaatgtt caacgcatca
    16741 tggaagaaat gggtggaggt ggccacttta acctagctgc cgctcaggta tatgaccaga
    16801 caatcgcaga agtatgtgaa aagttgacgg ataaaatccg tgaagaaata aatggaaatt
    16861 aggagaacag atatgaaagt aatcttttta gcagatgtta aaggtcaagg taaaaagggt
    16921 gagattaagg aagttccaac aggttacgcc caaaatttct tgatcaagaa aaatttggcc
    16981 aaggaagcaa caaaccaagc aatcagcgcc cttcgtggtc aggaaaaatc taaggaaaag
    17041 gctcatgcag agatgattgc agaagcagag gctatcaagg ctaagttggc tgaggaagct
    17101 actttagtga aatttgtcga aaaagttggt ccagatggcc gtacctttgg ttctattacc
    17161 agcaagaaaa ttgctgaaga attggacaag caatttggca tcaagattga taaacgccat
    17221 attcagctgg atcacccaat tcgtgcgatt ggtctcattg atgtaccagt taaaatctat
    17281 caagatgtaa taggcatcat taatttgagt attaaggaag cttaattatt tatctagcca
    17341 gtgaggaggt gagatcttgg cagagttgga tgaattgcgg gtgcaaccgc aggatattct
    17401 agctgaacaa tcggtattag gagctatttt ccttgatgaa ggcaagctgg tcttcgttcg
    17461 tgaatacatc gaagctgaag attttttcaa acatgctcat aagctaattt ttaaagctat
    17521 gattgctctt tctgatagaa atgaggcaat tgatgcgact accatgcgta actatttagt
    17581 gaaccatggt gatttggaaa atatcggtgg tttggcctat ttggctgaaa tcattagttc
    17641 cgtgccgacg gcagccaatg cggaattcta tgccaagatt gttgctgaaa agtctaatct
    17701 ccgtaaactg attgcccgtt tgacggatgc agttaatcag gcttatgagg gtgcggatgg
    17761 ttcagatgac attattgcca atgctgaaaa agctttgatt gatgtcagtg agggagccag
    17821 tcgaagtggt ttcaagcgga ttgatgatgt cttgaatatc aactttgaca accttgaaac
    17881 tcgtgctaaa cagacgtcgg atattacagg gattgcaact ggctatcctg ctcttgatgc
    17941 tatgacaacg ggtctgcatg aggaagagtt gattattctt gcggctcgtc ctgcggttgg
    18001 taagacggct tttgccctca atattgctca aaatatcggt acaaaactgg gtttgacagt
    18061 tgccatcttt tctttggaga tgggtgcaga aagcttggtt gaccgtatgc tcgcttcaga
    18121 gggagttatc aattcgaggg ctattcgtac ggggcatttg acgcaggaag aatggaataa
    18181 gtacatggtg gctcaagcta acctggctcg ggctagtatc tatattgatg atacaccagg
    18241 tatcaagatt acagagattc gttcgcgttc acggaagttg gctcaggaaa cgggcaatct
    18301 gggtttgatc ttgattgact atcttcaatt aattacggga acaggtcggg aaaaccgaca
    18361 acaagaggtt tctgagattt cccgtcagtt gaagattttg gccaaggaat tgaaagttcc
    18421 tgttatcgcc ctcagtcagt tgtcgcgtgg tgtggaacag cgtcaggata agcgaccagt
    18481 tttgtctgat attcgtgaat ctggatctat cgagcaggat gcggatatcg ttgccttcct
    18541 gtatcgtgat gattattatg agcgcggtga gcaggaagac ggtggtattc ccaataatac
    18601 ggttgaagtt atcatcgaga aaaaccgttc cggtgcgcgt ggtaccgtgg aactgatgtt
    18661 ccaaaaagaa tacaataaat ttgcaagtat ttccaagagg gaggatggat agagaatgag
    18721 tgatgcattt acagatgttg ctaaaatgaa gcaaatcaaa caagatatta aagaccatga
    18781 gggacagatt gtcgagttga cccttgaaaa tggtcgtaag cgtgagaaga ataagcaggg
    18841 gcgtattgta gaggtttatc agtctctctt tatcgttgaa tttgaagatc caaacaatac
    18901 ctttgtggaa tcttatactt actcagacat tttgacagaa aagattttga ttcattattt
    18961 ggattaatcg ctatataagg ctttacagag cctttttttt gtgctatact gatatttgtg
    19021 tgaaataaca gcagggcaaa atgaagctcg tcaacagaag gtcagcaaga agagtaatcg
    19081 ttgctgtttc gatgaaggcc tcctgcacga atcaggtttg cttaaaacgt tatttcctac
    19141 aaataatttt tatattttat tttaggagga cataactatg tcacgttata caggaccatc
    19201 ttggaaacaa gctcgtcgtc ttggcttgtc attgacaggt acaggtaaag aattagcacg
    19261 tcgtaactac gtaccaggtc agcacggacc aaacaaccgt tctaaattgt cagaatacgg
    19321 tttgcaattg gctgagaaac aaaaattgcg tttctcttac ggtttgggtg aaaaacaatt
    19381 ccgtaacttg ttcgtacaag ctactaaaat caaacaaggt actcttggtt tcaacttcat
    19441 gcttcttttg gagcgtcgtt tggacaacgt tgtttaccgt cttggtttgg caactactcg
    19501 tcgtcaagca cgtcaattcg taaaccacgg tcatatcctt gttgacggca aacgcgttga
    19561 catcccttca taccgcgttg aagttggtca agtgatctca gttcgtgaga agtcaatcaa
    19621 agttccagct atccttgaag ctgttgaagc aactcttggc cgtccagctt tcgtatcatt
    19681 cgatgctgaa aagcttgaag gttcattgac tcgcttgcca gaacgcgacg aaatcaaccc
    19741 agaaatcaac gaagctcttg tcgtagaatt ctacaacaaa atgctctaag atttgtatcg
    19801 ataatttaaa agccctattt acaagggctt tttgtctgta tttgaggagg ggattatgtg
    19861 gcgtaagtat tttttactag tgctattagc tattttggtc gtaggtgggg ccttctttct
    19921 atctcaatca gcggctacgc caaatgaatc agactatgtg aaggctgtaa agagttacca
    19981 ggctatttcg atagatgaag tggagcaaaa ggtccaagac gaggaagaat ttatattata
    20041 cataggtcgt gagacatgcc cttattgtcg tgattttgtt ccgaaattaa cagaggcagt
    20101 agagcgatca cgtgcgacta tctactattt ggatagcgag tcggatccga atggaaaaat
    20161 acaacaattt cgacagagtc aaggtcttgc tacggttcct agcctaacct attataaatc
    20221 gggtaaatta tctggaatac ttcgtaaagg gagtcaagca acgctatctg aaatagagaa
    20281 ttttttagct cttcaagggc agtaaatagt tttaagaatg tatgaagcga atatcataca
    20341 tttttttgtt catgtttgtg aaaatatttc aaaacagatt gaaaaacatt tcacaatctt
    20401 gtataatact aatatcagat aaaaattgga ggtttatatg aaaaagaaag cttggttagg
    20461 cttattaatg agtctacttg ctgttttgtt tttagtggcg tgtggaggta agtctgaaaa
    20521 gacagctaca tcttcatcat ctacgacttc ttcttcatca gaagaagctg tttcaggtgc
    20581 atctgagaaa gtttatacag atccatctga gttgaaagat agctacgatg ttatcgttgt
    20641 tggttcaggt ggtgcaggta tgtcagcagc tatctcagct aaagacgctg gtgctagtgt
    20701 tgctctcttg gagaaaatgc ctgttattgg tggtaatacg gctaaatcat cagctggtat
    20761 gaatgcttca cagactaaat tccaagaagc ggaaggtatt gctgatacaa acgataaatt
    20821 ctacgaagaa acgcttaaag gtggtaaagg gacaaacgat cctgaattgc ttcgttatct
    20881 tgtagataac tctgctagtg caattgactg gttggatgga atggggatca ctcttagcaa
    20941 cctaactaca actggtggta tgagcgagaa acgtacccac cgtccagcag atggttcagc
    21001 tgttggtggc tacttggtaa atggtcttta ccacaatctt gttgaacgta aagtaccaat
    21061 ttttgtcaat gcagatgtta caaaactcca agacaaggat ggagcagtga caggtgttga
    21121 agtgaagatt gctggtgaaa cgaagcaaat ctcagctaaa gcagttgtat tggcgacagg
    21181 tggtttcggt gctgatttgg aaatggttgc taaattaaac ccagcattga aaggttatgt
    21241 aacaaccaac caagaaggtt caacaggtga cggtattgca cttgctgaat cagaaggtgc
    21301 agcaactgtt gatatggaac aaatccaaat tcacccaagt gttgagcaag aaacttctta
    21361 cttgattaca gaagcagttc gtggcgaagg tgctatcctt gtcaactcaa aaggtgagcg
    21421 ttttgttaac gaattggaaa ctcgtgataa agtttcggcg gctatcaata gtttggatcc
    21481 aaactatgct tatgttatct ttgatgatgc cttgaaagat cgtgtgaagg ctattgctca
    21541 atacgaagaa aaaggtcttg taaaaacggg tgctacactt gcagatttgg ctaaagaaat
    21601 tgacctacct gcggacacac ttcaaacaac tcttgatact tggaacaagg ctgtggcaga
    21661 taagaatgat gcggcatttg gtcgtacatc aggtatggac catgctttga acactggttc
    21721 ttaccatgcc attaaagttg cacctggtgt ccaccataca atgggtggtg tgaaaatcaa
    21781 taccaatact gaagtattga aaacagacgg ttcagcaatt tctggtcttt atgctgctgg
    21841 tgaagtaact ggtggtgttc atggtcaaaa ccgtattggt ggtaatgcag tagcagacat
    21901 catcgtattt ggtcgtcagg ctggtgaaca agcagctgga tttgctaaga aataaattgt
    21961 agacataaga ggctcggcag aaagaaggtc gagcttcttt ttcttgtccc tctatctcta
    22021 agatgatata cttttaaggt atgaagaaga aaatagtatt attgttagga gctattgcag
    22081 ttatagtttt gacttggtct gctattgctg gtcagcgtca gcaatcttat gccgaggctg
    22141 aattaaggga ccaggataaa atctggatta tcactgattt gcattatttg tcacaggatt
    22201 tatttgatga tggagaggca ttttcttata tcgaaaagac agctgctggc aaggatttac
    22261 gttatggaaa agagcggatg gaggctctgg ttgagcaggt tgggcgtgaa catccttcct
    22321 tgttactagt cagtggagat ttgactctta atggagaaaa gcagagcatg gttgaattag
    22381 ctcagtattt tacagagatt gaagaacaag gaactgaggt tttggtcatt cctggcaacc
    22441 atgatattgc cagtggatgg gctagggcct ttaagggaga ccagcagata gtgacggatc
    22501 aggtcaccgc tcagcaattt gcagagctat ttgcccatca cggttatcaa caagctagca
    22561 gtcgtgacaa agcctcattg agctacctgg ccaaaccatt ttcaaatgct tggtttctga
    22621 tgatagacag caatatctac tctgacggct atggaaaagg agctcctcca accaatggac
    22681 gtatcaataa agagactcta gaatggatag aaattcagct tcaagcagcc aaggaaactg
    22741 gagtgagcct gatacctgtt gtccatcaca atgtcttgca acagcatgcc atgctttcaa
    22801 aaggctatac tttggataat gctgctgatt tgaagacctt gttcaatcaa tatggcattc
    22861 aatttggttt ttcagggcac actcattctc agaatattgt taaggaagac ttgggacaag
    22921 ttaactatac tgaagtagtc aatggagcat tttctattta tccagctatt atcggccaat
    22981 tgagccttga tgatacaagc attcattatc aaaaaactca acttgatatg gcttcgtggg
    23041 cagataagca ccaacctagt gatccaaatt tgcaggatca cgttacctac ttacaaactg
    23101 tctttgacaa taccagtgac atcatggtcc acaatatgtt aaacgatgaa ggttggtatg
    23161 atggtgaaac agcagatgct atttcacaat ttatcatgcc ggccaaccgt gcttattttt
    23221 caggagaaaa actagatcag acttggctag atgagaaggt atttccaagc caggcttatc
    23281 agaccttgca agcagcatca ccgagaagtt tcttggctga ttatcttgat gtgattataa
    23341 aacggagtca aaatagggat gtagaaaaac tagtgcttaa aacagaaaaa gattttgtcc
    23401 aatagttttc cacagattgt tgaaaaaaac cacaacttat ccacaagctg tgaataagtt
    23461 atgccgtagt aactatccga gtaggtttgt ttttacaaac aaaaatactc agcaactttc
    23521 ctgaaaatgt gataaaatag taacaaattg tgtcaaatgg agagaaagat gaccttacta
    23581 agcattgcta ttccgagtta taattcggag gcttaccttc attactgcgt tcattccctt
    23641 gtaatgggtg gggacaaggt tgaaattctg attattaacg atggttcgtc agaccgaact
    23701 caggaaatcg cagaggagct tgccaatcaa ttttcaaatg tacgggctat ttatcaagaa
    23761 aataaaggtc atggcggagc tgtcaataca gggatacgtg aggccaaggg gaagtacttt
    23821 aaagttgtag attcagatga ctgggtcgat accagggcct atttgaagat cctagagagt
    23881 ttgcaggctt tggaagatga aaatacgcca gttgatgcct ttatctccaa ctttgtttac
    23941 gaaaaagagg gacaatctcg caaaaaatcc atgtcttatc aatccactct acctgagaac
    24001 cgtatcttcg gttgggaaga cgtgggggca ttttccaagg ggcaatacat gatgatgcat
    24061 tccttgattt acaggacaga tttattgcgt gaggttggtc tcgttctgcc agagcatact
    24121 ttttatgtgg ataatatttt cgtatttact cctttgcagg ctgtgaaaac gatgttctat
    24181 ctgccagtag atttttatcg ttactttatt gggcgaaacg accagtctgt caatgagtcc
    24241 gtcatgatta agcgcattga ccagcagtta aaagtcaatc gaatcttggt agataattta
    24301 gacttggagg cgattgacaa tcctgattta cgaagttatt tgctcaatca tgtggagatt
    24361 acgaccatta tctcctgtgc ccttctcaat cgtgcaggaa cagctgagca tatgatgaaa
    24421 aagcaggaat tatggcacta tattcgggat aacaatccag ctctctttaa aattgttcgt
    24481 aaagggttac tcgggcaatt aaccaacctt tacggttacc caggtcgaaa aatttttaat
    24541 gccgtctata aaatcgcgaa aagaatttat ggttttaact aaaactcagt ttcggctgag
    24601 tttttctgtt ataataagga atatgaaaag cgtttacaaa ggagaagata aatgcaaaga
    24661 ttaggacaaa caggtctaga agtttcacga attggtctag gttgtatgcg aatggctagt
    24721 ctgacagagc aggaaactgc taaagtgtta gatacagtcg ttggacaggg aatcaacttt
    24781 tttgatcatg cggatattta tggtggtggt gaatcagaaa tccgttttgc tcagggggca
    24841 aaattggcgg gattgaaacg tgaggacctg cttctccaat caaaatgtgg cattcgcaag
    24901 ggttattttg atttttctaa agactacatc ttggagtcag tggacggtat tctcaagcgc
    24961 ttggatacgg aatatttaga cgttctcgcc ttacaccgtc cggatgctct aatggaagca
    25021 gaggaagtgg cagaagcctt ttatctcttg aaaaaagcag ggaaggttca tcattttggc
    25081 gtcagcaacc aaaacattta ccaaatggag ttattacaat cctatttcga tcagccccta
    25141 gtagttaatc aattgcagct ctctcctgct cacacgcctt tgattgatgc tggattacat
    25201 gtcaatatga aagatgatgc tgcgactatg cgtgatggtg gactgattga ttattgccag
    25261 cttaaaaaaa tcactattca ggcttggtct cctttcttga ttgatttgca acgaggtatt
    25321 tttgccaatc atccaaacta tgctctttta aatcaaacta ttggagaaat agcagagcga
    25381 tacaatgttt cacatgaaac aattgtcgtg gcttggattt tgcgtcatcc agcaaagatt
    25441 caaaccattg ttgggtccat gaatccggag cgcttaaaaa agattgcaca agctagtcag
    25501 attatcttga ctagaccaga atggtacgac atttatagaa gtgcgggcaa tattttgccg
    25561 taggaggcaa ctatgcagtg ggaaataaaa gcatttgacc aacttagttt acaagaactt
    25621 tacactatct tgacacttcg gaccgatgtg tttgtcgtgg aacaagcctg tccctatcca
    25681 gaaatagatg gaaaagatcc aagttgtctt catcttttag gaacagacaa tggggagtta
    25741 gtggcttatt tgagaatctt gccagctggt ctaagttatg atgaagtttc catcggtcgt
    25801 gtggtcatca agccaagcca tcgtggtaaa ggattgggtc ggccaatgat ggagcaagct
    25861 atcgcttaca ttacaaccga gtggaaggaa agtcagatta aaatcggggc ccaagcctat
    25921 ttggaaaaat tctatcaatc gcttggcttc gaacctattt ctgaagtata tttggaggat
    25981 gatatcccac atttggatat gttataccgt aaactagtcg tttaaggctg gtttttcgat
    26041 ttttctcggc aatttccccc aaaaatcagc cattttatgg tagaatgaat aggtataaaa
    26101 tcagtaaaag aggaagaaaa tgaaacgttc tatgtacgct ggacgtgttc gtaaggaaca
    26161 tgtcggacag gaaattactt tgaaaggttg ggtcggccgc cgtcgtgact tgggtggcct
    26221 gattttcatt gacttacgtg accgcgaagg cattatgcaa ttggtgatta accctgagtc
    26281 agttgaagct gaagtgatgg caaaagcgga gagtcttcgt tctgaatttg ttatcgaagt
    26341 aacaggaacg gttgtggagc gtgagcaagc taacgacaat atcccgacag gagctgttga
    26401 actgcaagta accagcttga cagtgttgaa cacggctaag acaactccat ttgaaatcaa
    26461 ggatggcatt gaagccagcg atgacactcg tcttcgttac cgttatttgg atcttcgccg
    26521 tccagaaatg ctcaacaact tcaaattacg tgcggctgta acccacagca ttcgtaacta
    26581 cctagacgag cttgaattta tcgatgtgga aacgccaatg ttgaccaagt caacgccaga
    26641 aggtgcgcgt gactacttgg taccatctcg tgtgtccaaa ggtcatttct atgctttgcc
    26701 acaaagtcca cagattacta agcagctttt aatgaacgct ggttttgacc gttactacca
    26761 aatcgttaag tgtttccgtg atgaagattt gcgtggtgac cgccaacctg agtttacaca
    26821 ggttgacttg gaaacctcat tcttgaatga ggttgaaatc caagacatcg tagaaggctt
    26881 gattgctaag gtcttgaaag ataccaaagg aatcgatgtg accttgccat tccctcgtat
    26941 ggcctatgac catgctatga acttctatgg ttcagacaag ccggatactc gttttgagat
    27001 gcttttgcaa gacttgacag aacttggcaa agaagttgac ttcaaggtct tctcagaagc
    27061 accagttgtc aaagccattg tggtcaaggg tgcggcggat tgctactcac gtaaagatat
    27121 tgataaatta acagactacg caaaacaatt tggtgctaag ggacttgctt gggttaaagt
    27181 agacaaggga gaattagcgg gtccagttgc taagttcttg acgggtatta cagaaaactt
    27241 gacagcaagt ttacaacttg aagacaagga cttggttctc tttgtagcag atgaattgga
    27301 agtagccaat aataccctgg gagctttgcg taaccgcttg gcaaaagagc aaggtttgat
    27361 tgacgaaagt aagttcaact tcctctggat tgtagactgg ccaatgtttg agtggtctga
    27421 agaggaaggt cgctatatga gtgcccatca cccattcacc ttgccgacag aagaaacagc
    27481 tcatcatttg gatggtgatt tggcgcaggt gcgtgcagtt gcctacgata tcgttttgaa
    27541 tggttatgaa ttgggtggcg gtagccttcg tatcaaccaa aaagacatgc aagaacggat
    27601 gtttaaagcg cttggcttct ctgctgagga tgcccatgag cagtttggtt tcttgcttga
    27661 agctatggac tacggcttcc caccacatgg tggattggct atcggtttgg accgctttgt
    27721 catgttattg gctggtgagg ataacattcg tgaagtaatt gcctttccga agaacaacaa
    27781 ggcatctgat ccgatgacac aggcaccgag cacagttgca tcagcacaat tagaggaatt
    27841 ggctttaaac attacacttg aaaatgaata aatttttcag aaaacagcgt gtacgctttt
    27901 taagaaaggc cagaaaaaat aagttttggt cggtcatttt gggaatttct cgggaaaaat
    27961 atgccgaacg gatatcgggg tccattattt atggactcct atctagtatt gcagtcaatt
    28021 ttttctttag accagggaat gtttattcgt ctggagttac tggtcttgca cagattgttt
    28081 ctgctctggc agcttcttac gctggttgga atgtaccgat tgcggtggtc tactacggcc
    28141 tgaacattcc cttgcttatc ttagcttggt ataaaattgg ttataaattt actatcttta
    28201 cctttattac ggtatccttc agttctttct ttatccaatt tatgccgcca gttaccctga
    28261 cgacggatcc gttagtaaat gctattttcg gtggtcttat gctgggaact gggattggct
    28321 ttgcacttag gaacaatgta tccagtggtg gtactgatat tgtctctatt attattcgca
    28381 agaagacggg acgtcaagtt ggctccattt ccttgattgt caacattatg attatgttga
    28441 ttgcagggat gacctttggc tggcaatatg ccctgtattc aatggttgcg ctgtttatct
    28501 ccagccgcat gacagatgcg attttcgtga aacaaaaacg gatgcaggcc atgattatta
    28561 ccaatcatcc tgaacgagtc attaagaaaa tccataagaa gctcaatcgc ggtgtgacca
    28621 ttatcgacgg tgctgagggt gcttataacc atgagcaaaa gacgattctc attaccatca
    28681 tcactcgtgc agagttcaat gatttcaagc atattatgaa aaaggcagac cccaaagcct
    28741 ttgtatcggt agctgacaac gtcaatatta ttggtcggtt tgtggaaagt gactgattga
    28801 caaacactac atgtaggagt aaaatagtat ttacagtcta catgtagtgt ttttattata
    28861 aagaggaaat gtatgaagaa gaaaatatat ttggtttaca tcagtttgag tggcaataca
    28921 gagagttttg tgaaacgtct aaagtccttt ttccaatttc aaactgactg ggagattgag
    28981 ctggtccatg ttaaagattt ggtcaagcag gatattcctt tctatcagct agatgcaccc
    29041 tttgtcgcct ttttaccaac ctacttagaa ggtggaaatg gagtggacaa cggagatgtg
    29101 gaaattctga ccaatcctct cggagatttt atcgctttgg ggaccaatgc agagctctgt
    29161 ctgggtgtcg ttggctcagg caaccgtaat tttaacaacc aatattgtct gactgctaag
    29221 caatatgctg agcgatttaa ttttcctgtt atagacacct ttgaactcag agggatgcag
    29281 aatgacattg aacgcattgg tcggaagata atggaattga tgtaaagagc ctcaactcag
    29341 ctttatgctg ggttgttttt ttggtcacac aaaaaaaact caccagcgtg accgatgagt
    29401 tttaaggaga ttatgaaaaa tatttaggat tgactatagt atacctcaga ctatatcgtg
    29461 tttcatcggt ctaaagtcct atttgtcttc taaaatgcaa atactctgtc ctgcaatcgt
    29521 gatttcttgc tgatcacgct cttcgattgg aggatagttg tagagaactg ccttatcttt
    29581 ttcacgttca taggtaaagt cgctatccgc aaagttgatg ataaattgaa tggtcttttc
    29641 ttcacttgtc acttgatagc gaagaacata ttcagttaac cagtagaaat cacaagtttc
    29701 ttggatactt tcgtagttat cctggctgag tattggatta ttttttcgga aggtaattaa
    29761 ttcctgaatg aactcgatat gttccttata acgaatagct cgtgtccagt ccagacggtt
    29821 gatacggtca gggacattgt aagtattatc tatttcatct tttgtacgga aaaattcctg
    29881 tccgctgtga atgaaggcca taccctgaga gattaagatg agctggagac caaaacttgc
    29941 agcacgtttc tgctgttgag gtgtccagtt tggattctca atatggaaat agtcaaatgc
    30001 tgtagcatta tcatggcatt cgatgtagtt gagggactgt tgaggcgtta agaaatggct
    30061 taatcggcta ccagtcagga gatgttgtac cttttcgtgc aattgcttat ctactagacg
    30121 gtttggattg agcaagagtt tcttaaaggt atcacggtag tcgtcattga agaagctgat
    30181 ttcaggtaac tgctccgcat tgtactggtg agccagcttt tcaaattcga ggcctgttgc
    30241 cattttccat ccttcaccat agaggtagac atttgggtga atggcagata attcaatttg
    30301 aatctggttg attgtttcgc tatctaaaat acccataagg tcgaaacgga agccatcaaa
    30361 accatagagt tctagccatt ggcgaaggga ttgtttgatg tagttgcgga ccatactgcg
    30421 ctcgctggca acgtcgttac cgcagaatgt cccgtctgtt cgcatgccgt ggtcatcata
    30481 gcgatagaag tagcctggaa cgatgagctc aaatgcgtac ttttcagcat gatagacgtg
    30541 gttgtaaaca acgtccataa taactgaaat atctgcatca tggtaggcct ggatagcttc
    30601 ttgtagctct agaatacgtg cgtaaggatc gtttggttgg ctagaatagg aaccttctgg
    30661 tagattgtat tgcatagggt cgtagcccca gttgtaaaca gcttggggtt tattttcatc
    30721 cacactgcca aaatcataaa caggcaataa ttgaatatgg gtaacgccta gttctctgat
    30781 gtaatccatt cctagcttca ttccatccat ggtaggagat tctgtcaatc cgagaaattg
    30841 actgcgatgc ttgaacccag cttctttttg ccatgagaaa tcacggacgc tcatttcata
    30901 gataatggcc tgagaaatag gtcgttgggt cttagcacga cgaattttat gaagtttatc
    30961 tggattgata acgtagctgt cacctgagtt ggctgtggaa gacagggcgt agggatcgtg
    31021 aacggaaatc cattcactgt ttactttgtg gagatagtga tagctgtgcg cttctaggtc
    31081 tccttcaaca gtaatttccc aaactccctt tgctcccttc gtcattgcat gtgggtttcc
    31141 ttctagaaca agaaaaactt gcttagaaat aggtgcccat aacttaaagg ttgttgcctc
    31201 tggtcgatag gttgctccca agtcttgccc attgtatgta taactttgtt caaaaagtgt
    31261 agagcgaacg atatgaccat agccgagttc tgttttatta cggtcttggt cgtagatggt
    31321 atagtccttg tccaatgtca gtgcatggag gctggttaga taatagatga cgagattatc
    31381 tacttctaga caggaatgga taaaaagttg agatgtagca tcatttgatt caagtgagaa
    31441 gctcatatgt tcggagtcga atcgtttttc catgaccaag cgaatagttg ctacatcgtc
    31501 aagagatgct tgaaattgtc gtaatgccat gagccaaaag gctcctttct aagttagagt
    31561 tcggtaatgg tatgtggaat gacacgtcgg tagcagactt gcttgtcttc cttgttgtca
    31621 ttgatgattt ggtgcagagt gttgaatgcg acgcgtccta gtttaatagc gtggacgtca
    31681 acgtaggctt caatatctag ttttggcttg acggagtcaa aagaaatgat aggaagtttg
    31741 actccgactt cgttgagata tttgacaaca ccttcggcaa cggaagtgtc agttgtgacg
    31801 attgcatcca ggtcttgttt gagtaatttc ttagaaattt tgtaggcact gtcttcgaga
    31861 aggaagccag acacaaactt gaccttgttt tcatccaggg gaatattgtg cgatttaagg
    31921 gcgttcttgt aacctgtata acggtcttga gatacgacta actctttgtt accagcgacg
    31981 aaggcaatgt ttttgtaacc tttattgatg aagtagttgg tagcttcaaa gccggcctgg
    32041 atattatcat tatcaactag tgatatgaat ggagaatccg ccttaccaag aatgaggaag
    32101 gggaatttgt gttggatagc taattgtacc aatggatcgt cgggcttgga atagaggaag
    32161 atgaggccgt ccactcgttt gccgtagacc atctgagaaa tcgcttctag acgttgttct
    32221 tcattctttc ctgtggagat ttggatggca taattatgat ctgccgcaac ttgagaaatt
    32281 cctcgtaagg cagtagggaa gaaggggttt tgatagaaaa catccgagtc atctggcaaa
    32341 accagtccga taacctgtgt atagctagaa actaggcttc gagcgttgag gtttggatgg
    32401 tagttgagat ccgccatggc tttcctaacc aagtctttcg ttttttggct aatggcagag
    32461 ctattttgaa tgacacgagt aactgtagat ggcgagacac cagctaattt ggccacatct
    32521 ttgatggtag cacgcatata gacctcctag atttcatctc tgaattttgt tttgaaaaat
    32581 acccataaca agcatagcac aaataggata ttttgtatgg ttagaagggt agtcacaggt
    32641 tgaccaaata gtccaattag acaggctata agagtaggca ggcccagaca gttgagagca
    32701 aaattatagc actctgcaaa cgtttcaaaa tggaagaaac gagacttttt agtcaaaaag
    32761 aggaagaagc tggctcctag taggatgaag agtagttttg ttgttaaaat aaaggtcgaa
    32821 ataagagtga tggacagtcc gacagctgct cggttggctt gataccattt tttagagatg
    32881 gttgcagtca aggtctcctt actagcaaaa tcgtcagtat tcaaaccttc ataagtcaat
    32941 tcgcttagaa tatcacttcc tttacgaata gtcagagact gattggcgaa ttgataagta
    33001 aagtcagaac tagagctaac ggtatcacca aatgtcactt gctcccagtt agtatttcca
    33061 gtataggtta ggtggttgtt ttcaatgact gcatattgac ggaggtcatc tacgacctga
    33121 tctgtcagag gtgcatagac accttctacg aaattttcta aaggataggt ttcgagattg
    33181 acagtctgta gggagctcgg aatgagcaag agcgccacta aaaaaagcgt tgtaaacaac
    33241 cgttgccaaa ggttcattag tctacgattg gcaaacatat ttcttggaga gaagatacta
    33301 gaaaaatagg aaaaaggata tgggagcatt cgttggacct ttcataaata tcaatgtttc
    33361 ccctaaaaag ggaaaaggtg ggacagggtt atcccttgtc gccaccgctt gttaaaccag
    33421 atacaaagtt cttttgtagg aagaagaata ggatactgat agggagggca ataagaatgg
    33481 cacctgccgc aaagtaggca atcttcatat tcttcacatc actgatgaag gtttgaagtc
    33541 cgacagctac agtatagaat tctttttcac gaagcaagaa tttggatagg atgtaatctc
    33601 caaaaggtcc catgaaggcc cagagcgctt gtacagcaat cattggacga acgagtggga
    33661 ggacgatttg ccagaaacgt ctgaagtgac cggcaccgtc tagttttgca gattcatcaa
    33721 gtgagattgg caccgtatcg aagtagcctt tcatgagcca agcgttcatt gggataccac
    33781 caccgacata aaggaagatg aggaaccagc tttggttgag cgcattgagc atgagggcca
    33841 taacgaagaa ggcggtaaga gcagccatgg ttggtaccat ttggataatc aagaagaaga
    33901 ccaaactctg cttacgagcg atgaagttat agcgactata agcgtaaccc gctagtacca
    33961 ctatacttgt ttgaataacc attgtcaaga gagcaatgat aagcgtattg aagtaccagg
    34021 ttccgtatag agtctcgcta aagagcttgc taaaattgtc tagtgtaaag ttgatattgc
    34081 tatccaattt gaaggcaacc acgttaccag acttgaaggc ggacatgatt gtaatcaaga
    34141 gaggatagat gatgataaca gacagaacaa tcagatagag ataggttaag gtctgattga
    34201 gtatgcgtct agtctttaca gatactttca tcttatacat cctccatttc aaaagcattt
    34261 agttttttga aggcaatcat ggaaatactg atgacaatca ttgagataat caatgtaact
    34321 gctgccgcca ttgagtattg aggtgatgta cctgtggtta atttataaat ccatgaaatc
    34381 aagatgtcag ttgaacctgc tccaccaccg acgctaccag gaccaccatt gttgaagagg
    34441 tagatgatag agaagttgtt gaagttgaag gtgtattggc tgattagtgt tggagctgct
    34501 actgctaaaa tcatcggaag ggtgatgtta cggaattttt gaatggcact tgcaccatca
    34561 atgtaagcag cttcatacaa gtcgtttgga atggattgca aaatacccaa ggtcaaaaca
    34621 tagatataag ggaaaccaag ccaagcctgc atcataatga gagcaacttt agtccagaat
    34681 gggtcggtct tccaagggat aaggaaattg tcgatgaatg gtaggaattt gctcaggaat
    34741 ggcaatacct gtgtgttaat agcgccgaca ctatcgttga acatgttact aaaagtcaag
    34801 atagttacga aggccggaac agcccatggg agcaagaaga taacaccgaa gatacgtttt
    34861 cctttgatga atggttgatt tgcgatgatg gctgtcaaga taccgataac aatttgagct
    34921 gttgaagcgc agagtgccca gataattgtc cagccaagta cagaaccaaa tgctgtacgg
    34981 aatgtgctta gtttccagat atttgtaaag ttggtaatac caatccaatc caacaatgcg
    35041 cttggtggca ggtgtttgaa gtcgtagttt gtaaaggcga taaacaatgt aacgacaact
    35101 gggaagatga tagcaaaggt catcgcaata tatgatggga taatcaagag atatgggaaa
    35161 ccattctcat agataccttt taccatttct tttaatgtgg tagccactgg catccctgaa
    35221 ttccatcgtt tcgcggtatc acgtgcgtct tttaggttga gtgcatagaa agcgagatag
    35281 acaactgtga taattaagtg gaaagaacca cgaatcagca tgaatagcga attatcttgt
    35341 ccacgaacac taccgagagt aatcaagttg cctaattcac cagctgcaat agcaataaaa
    35401 taaacaagga aggcaagcgt aacacctaaa aagatgtatc ctttggcttt ttgtttattg
    35461 tagatttgtc cgagaccagg gatcagggac aaaaggagtg ccttacctgg attttgtttc
    35521 atagtcatag tctccttata tagcttctga atagacggtg gatgttatcg tcatttagtt
    35581 tttcttttat tacttcgtta actcgccttg tctaacttca gttatgcctg cggctcattg
    35641 cctcgtacta aaagtaaact aaaagactat atctattcag tttatcgtta agtaataaag
    35701 tagactggga agagaactcc cagtccaact ttaaacttga ctagttgctg tgtttttgag
    35761 caatttcgtc tgcaatagct ttcacagcat ctgttgcagc agtcttagcg tcttttgaac
    35821 cacttgcagc ttcgaagagc atgttaccag ctggtgtcca aactgaaccc atttctgaga
    35881 tgtttggcat tggttgagct gaagcaaact ggttgataac tgcagttgtc aattcgtctt
    35941 ttttgcttac agcatattca cgagcttcag tgttagctgg aacttcgttt gtagcgtcgt
    36001 aaagtgcttt ttgttggtct gtagcagtca agaagtcaac aaacttttga gccatttctt
    36061 ggtttttagc gcctgctgga acaacccatg ctttaccacc accgaatgct gagtagtttt
    36121 tgccgtttac aagagttggt attgttgcta caccatagtt tacaccagct tctttatatg
    36181 aagctgcttt ccaaggacct tcgatgatag cagctgcttt accgtctgta aattgagtgt
    36241 taatcaagtt gtttgcagca gtaccgtctt gaagaccttg aggccatttt tcgtaccaag
    36301 ttttagcgta ttcaattgct ttgatggcac cttcattagc aagtccgatg tcttttggat
    36361 ttgtaccatt ttcaccgaat acatagccgc cgtaaccaga aagaagtccg tatgtatagt
    36421 agaaattcgt ccagtcagct aggaatgctg aagttttacc ctcttcacca gcaaatgcgt
    36481 atttgctgtc tttagcaagt gtttcaagtt ctgcaaatgt cttaggagct tcagtcaaca
    36541 agtctttgtt gtagtaaaga acaagtgttt cgataaccgc aggtgaaccg taaactttac
    36601 caccgtttgt tacaagagca gttgttgtgt catctgcttt gctatcatct gcaagtgtca
    36661 attcagatag ttgaccttca gaaccaaggc tacctacacg gtcgtatggt gccatcataa
    36721 cgtctggggc tgaacctgat tggttatcca atgataagtt atccaaacca gttaaagcat
    36781 caccagtttt aacggtaaca gatacaccat tttctttttc gaagccagct ttgacatcgt
    36841 tgatgtaaga ttcataacct tggtcaacgt agacagtcaa ttctttgcta ccttcagcag
    36901 acttagatgc ctctgttgat gaacctgagt ttgagcatgc agctaaaaca gcagttgatg
    36961 cagcaagaag agcaacgctc ttaaggagat tgtgtttcat tttctttcct ccgaagataa
    37021 atagttttgt gtgacgcttg ctttgcgcaa acgttttcct aagttgactt cattatacac
    37081 tagattgaat ccgtttgcaa gcgtttttat aaaaattttt aaaaattttt ttgagtttaa
    37141 aaaaatataa gaattttttt gcacatattt aatgaaaaga aatatggaat ggcatgtgaa
    37201 ctaacagaat taaatggaaa ttccttgatt tgttaccgtt ttcagtatat aataaaaaag
    37261 agaaaacgtt tgcgttgtgc aagcggatta gataggagaa cttatgaaag taagacagag
    37321 tggtgtgctg atgcacattt catcattacc tgggaaatat ggaattggtt ccatgggaca
    37381 atcggcttat gaatttgttg attttttggt aagaactagg caacgctatt ggcaaatctt
    37441 acctttggga acaaccagtt atggagattc tccttaccag tcattttcag cttttgcagg
    37501 gaatactcat tttattgatt tcgatatatt gattgataaa ggctggctag cagcagagga
    37561 tttacacggt gttgactttg ggcagaatcc tacagaggta gattacgcag ccatttttta
    37621 tgctcgccgt ccattactgg agaaggtagt tgtcaacatg aaggcacaag gcttgccaga
    37681 agattactgg atgttcttag gggagaatga cttttggatt catacctttg cggagtatat
    37741 ggctataaaa gagcattttg acctcaaacc ttggacagag tgggaagatc aaggggcgcg
    37801 cttacgttac cacgatacct tggaatatta tcgtcacttg ttggcagatc gaattgatta
    37861 tcaccgcatt acacaatatt tattcttttc acaatggaag gccttaaagg cttatgccaa
    37921 tgccaaaggt gttgagtttg ttggggatat gccgatttat atcgcggctg attctgccga
    37981 tatgtgggct aatccacatt tctttaaaac agatgaagaa ggcaagccga gtgtggttgc
    38041 aggatgccca ccagatgcct tttcggaaat tggtcagctt tggggcaatc cgatttatga
    38101 ttgggaagcg atgaagcatg atggatttac ttggtgggtt gctcgtttac gtgaatcctt
    38161 taagatatac gatatggtac gtatcgacca ctttatcggc tttgcctcct tctgggaaat
    38221 tccagcgggt gaagaaacag ctgtaaatgg ctatcgtgtc gaagcaccag gttttgagtt
    38281 atttgataca atcaaacgtg aactcggaga cttaaatatc atagctgagg atttgggctc
    38341 tgtgacagac aaggtcatcc aattacgtga tacgacaggt tttcctggta tgaaagtcct
    38401 gcagtttgct tttgatccag aaggagacag cattgaaatg ccacataacc atcctaataa
    38461 tgtggtggca tatacgggaa cccatgacaa tgatacgatt cttggctggt acgaaaatga
    38521 aacaacgaaa gaatcgcgtg cattcttaga taagtacagc aatcgtcgtg agggcgaaac
    38581 ggtcagtcat gctcttttcc gcctgctctt tggttcacct gcctttatgg cggtggctac
    38641 tatgcaggac ctacttggtt tggatggttc agctcgcatg aacttgccaa atacgcttgg
    38701 tggtaactgg acttggagaa tgacggcgga tcaattgaca ccagagattg aagcggaatt
    38761 gctagacctt acagaaactt atcgtcgggt taatgtgcat ttggttgatg aaaagtcata
    38821 ataaaatgaa ctcctctaag tataacttag aggagtcttt gttatctctg ttgtcggaca
    38881 tagagccagt agcgaatcca gtagtagatg gtgtagagta ggcaggctac gatagtccaa
    38941 atgaaaaatg tattcagcac tttagcaaga cttggcaata accagttaat cagtagagtg
    39001 actacaaaaa taatcactcc gatataggtg ggcaatttct tcatgagttt tcctcctttg
    39061 aacttagtat aacaaaatta tatttgcagt caaatgaaat atatgattga caatataaaa
    39121 atatgagtat aaaatataga aaaaaggagg ctattatgaa aagaccaatt catttgtata
    39181 tctttgttgt gctgtcatct attgcgagcg tattgcgtgt ctttaatgta ttttttgcga
    39241 agtacaatga ggaagctgtg cgtcaattgt tgcagaattt taatgtagag gggcttgatg
    39301 aggtatattt tacctatatg atagagtctg tcaatttcca aacgaatctt gtcaataagg
    39361 catttgcagt tgtgttatta cttgctgtga ttgcgacgat tgtgctgctt ttcctcaaga
    39421 aaaatgagca ggcttcttat acctacctgg gttatctttt tgtaactctc ctattttcga
    39481 cctatgcttt tattggtgag aaagggttga gtcaaattta tactgattca gttatgcgtc
    39541 agtcagtaga ggcgcaggca atgatgaatt atattattcg agttgtccta tttgccattt
    39601 actttggtgt gaccgtcttc ttccatctgc gtaaaccaaa ggaaaaacca agcacagcta
    39661 ttaattcgac agatatttaa tgaaaaagca cagtctaaaa agattgtgct tttaaatatt
    39721 gtcttttttg tgattaggag atggcgatgt agcgtcgagc acctgagtaa ctgatatagc
    39781 taatccatgt gcgaccttcg gcagtaataa ctttatcata gcgaacgact gagccagctc
    39841 cgtagaaggt gatttcagga ctggacagac ttggattgtt gcggacacta gagtaggact
    39901 ggatggtgta gctacctgat gctggtaggc tgttgtttgt agattgagta atggtaggct
    39961 ggatggcgat atagcgccga gctccagaat agctgatata gctaatccag cgttgtccgt
    40021 cggcttcgag aaccttgtcg taacggacgc tttcgccagc ataatagtaa tcgagggtag
    40081 gactagatag actagcttga gatttgatgg ccagttggct ggtaaaagta taggtgccgc
    40141 ttggggctac ggcgggttga gagttggttg ttcccgctcc gaggttttta aaatgaatgt
    40201 agccgctaac ggcatttttg gggataactc gccttccgta ggtatagcga acaccgtagt
    40261 tgtattcctc aatctctacg gtatctccat ttactgcaga aacccatgcg acatgcatgg
    40321 gagtttccca ccaggcaacg gctccgacag caggtgtcat attaacttga taaccttctt
    40381 gtttggcacg atggccccaa acattggcat caccataggc aggtggcaat gtaaaaccgt
    40441 taactgtgct aagtcgaaag gctacaaagg aggtgcactg gcgtgtgtag agtctccatg
    40501 gatcgactgc atcaactact gtgtagggat agtcatctcc tcgtgcattg gctgaggctg
    40561 aaggtgggga ttgtaaattg ataccgacaa taaaaatggc ggctagaagc tgacaggcga
    40621 ttccttttct tctcaagaat gttttcatta tttctccttg tgtagtagat gtagaggtat
    40681 cacgtgtacc tcccatctat atattcgtaa ttgaaatgaa tctttttgat tttcatttgg
    40741 gataaggagt tgttgtgata aggaatcgga aacgagagta ggcaacgtgg tagaaaaaaa
    40801 gcctcggctg atggccgaag gctttgatta cattttttct ggtgctttta cgccgagaag
    40861 acgtagagct tctttgagga caagtcctgt tgcgtaggca agtgctagac ggctatcacg
    40921 ctctgggctt tcatccaaga tacgagtgtg tgcgtagtac ttgttgaagg cttgcgccag
    40981 gttgatagca tatttagcaa tgagggatgg gtcaaatttg tcaccagcac gttctacaac
    41041 gtttgagaag ttttggatgt gcttgatgat gtcccagctt tctgcatcag ccagtttgta
    41101 gtcattttct gcgcttggta caaagtttgc cttgcgtagg atagactgga tgcgggcgta
    41161 tgcgtattgt acataaggac ctgtttcacc ctcgaaggaa accatagctt ctaggtcgaa
    41221 gtcgtagccg ttgtcacggt cggttttgag gtcgtagaac ttaacagcgc ctacaccaac
    41281 tgcgtgtgct acttcttcct tgttttcaag gtcagggttt ttagcttcga tttgagtaag
    41341 ggcgcgtgaa atagcttcat ctagagttgg ctcgagtagg atgatatttc ctttacgagt
    41401 ggatagcttt ttcttgtcct tggtaaccag accgaaggtg acatgggtca tatcgtcgct
    41461 ccagtcgaaa tccatttctt tcaagacagc cttgagttgc ttgaagtggt tgatttgctc
    41521 ctgaccaacg atgtagatac ttttcacaaa gtcgtaagtg cgtttgcggt acatagctgt
    41581 tgccatgtca cgtgtgatat aaagtgttgc accgtctgtt ttcatgatga gggctggtgg
    41641 gagattgtag ctttcaaggt caacgattct cgcaccttta gattcttgaa gaagtccttt
    41701 ttcttctaag atttggatgc cttcgtccat cttatcgttg tagaaagctt cgccattata
    41761 gctgtcgaaa gttacatcga gtttatcgta aatgcggttg aattcgacca agctctcatc
    41821 acggaaccat tgccatagct cgtgtgcttc tgaatcgcca tcttccaatt ttttgaacca
    41881 ttgacgtgct tcgtcgtcca actcaggctt ttcttccgct tcagcgttga tacgcacata
    41941 gagtttgagg agttctgaaa ttgggtctgc ttcgaccgca gccttgtcac cccaaagttt
    42001 gtaggcaaca atcaacatac caaattgttt accccagtca cccaagtggt tgatgcggat
    42061 tggcttgtag ccaagctttt cgtggatgtt ggcaagggca tcgccgataa cggttgagcg
    42121 caaatgtcca actgagaatg gtttagcaat gtttgggcta gacatgtcga tggttacatt
    42181 gcgtccttga ccaatgttga gtttaccgta ttggtctttt tcagtaataa catctgtcaa
    42241 gacttggtgg gaaatcgcag ccttgtccaa gaagaagttg acgtaaggac cggtagctac
    42301 aaccttctca aagccagttg tatcgagttt ttcaacgatg tccgctgcga tagcctgtgg
    42361 agccttgcgt tccactttag caagtgagaa ggctgggaag gcaatatcgc ccatttctga
    42421 tgattttggt ttttctagca gattgtagat agcttccact tccaaactcg gaaggatggc
    42481 agccaatctt tctgcaatca cttgtttttg attcatatag gatttcctcc aaagttattg
    42541 attctatttt aacacatttc taggctaata gcggggaaaa gtgttataat atttctataa
    42601 atatacattt tgaggtaaaa tgatgaataa agcaggacga catgatttaa taaaggccat
    42661 gattcgtcaa gaaaaaatag ggcgacaaac ggatattcag caaggtttag aggctcgagg
    42721 ggtagtggta acccagacaa ccttgtctcg tgatttacgt gagttgggag tgataaaaat
    42781 tcatgaaaat gggcgttcat tttattcctt agctatcgaa gaagagcgcg tgaattttat
    42841 ccaattattg gcacaatatg cgtataaagt agatagggct agttttattc tggtccttca
    42901 ttcagaactg ggagaagcgg ccttgatggc caatatcatt gatgcagaaa agccagagac
    42961 tattttggga acgctggcgg gagctaatac acttttagtt atttgtcgag atgatgtagc
    43021 ggctcagcaa gttgaagcgg agattaacta ctatttgaat tagaacaggg agagatgatg
    43081 gcaacagaaa aaatttctcc gggaatgcag cagtatctgg atataaaagc acagtatcca
    43141 gatgcttttt tgcttttccg tatgggagac ttttatgaat tattttatga agatgcggta
    43201 gaagcggcgc agattttgga attgtccttg accagtcgga ataagaatgc ggagaatccg
    43261 attcctatgg cgggggtacc gtatcatgcg gcacaacagt atatcgacac acttgttgaa
    43321 ttggggcaca aggtcgcaat tgccgagcag atggaagatc ctaagcaggc agtcggcgtg
    43381 gtcaagcggg aagtagtgca ggtcattact cctggtacgg taacagactc gtccaaaatg
    43441 ggagcagaca gtaactattt ggttgcgctt gaccgtcagg gagtgcagtt cgcactttct
    43501 tatatggatg tgtcaacagg tcagtttttt gtgactagtt tggatgattt tactagtctt
    43561 tgcggtgaaa tccgcaattt acgggcacgt gaattggtca tcggctatgc tttatctgag
    43621 gaagaagagc aggtcttttc aaaccagatg aacctcctac tctcttttga agatgaggtg
    43681 acagaggatg tccaattgat tgataactcc ttgacagatt tggaaaaggc tgcggcgggc
    43741 aaactcctca gctacctaca tcggactcag atgcgagatc tgagccactt gcaaaaggtg
    43801 gtccactatg aaatcaagga ctatctgcaa atggactatg caaccaagtc cagtctggac
    43861 ctgcttgaaa acggtcggac aggcaagaag catggtagtt tgtactggtt gttagacgaa
    43921 actaagacag ccatgggtat gcgactattg cggacatgga ttgatcgtcc cttgattgac
    43981 cttaagcgga ttgagaatcg tcaggctatc gttcaggttt ttctggatta cttctttgaa
    44041 cggagtgact tggttgaagc tttaaaaggt gtctatgaca tcgaacgctt ggctagccgg
    44101 gtatcttttg gaaaaaccat gccaaaggat ctcttgcagc tctcccagac tctgggaaat
    44161 atcccagcaa tcaagaatat cttgcaacaa atcaatgagc ctgctctagg caatttggtg
    44221 gctggattgg acccaatccc agaactgcac gctctcatca gctctgctat tgacccagaa
    44281 gcccaaggga ccatcacaga tggaaacatc atccgtacgg gctttgatga aacgctggat
    44341 cagtatcgtc tggtcattcg tgagggagcg ggttggattg cggagattga ggctaaggag
    44401 cgtgaagcgt ctggtatcaa taatcttaag attgattaca ataaaaaaga cggttactat
    44461 ttccatgtta ccaattccaa tctgggcaat gtgccggacc attttttccg taaggcgacc
    44521 ttgaaaaact cggagcgcta tggaacggaa gagttggcta agattgaagg gcagatgttg
    44581 gaggcgcgtg ataagtctgc taatttggag tacgagattt tcatgcggat tcgccaagag
    44641 gttgagaagt atataggtcg cttgcagaag ctggctcgga ccattgcaac catcgatgtt
    44701 ttgcaggcct ttgcagttgt ggcggagcag caacatttgg tttgtccacg ctttacagat
    44761 caaagagaac tgaccattga ccgtggtcgt catgcggtgg tggagaaggt catgggcaag
    44821 cagacctaca ttcccaactc tattcacttg aataccgata cgcatatgca actgattact
    44881 ggtccaaaca tgagtggtaa gtctacctat atgcggcagt tggcggttat cgtcatcatg
    44941 gcgcagatgg gttcctatgt gcctgcggat caggctgaat taccgatttt cgatgctatt
    45001 ttcactcgta ttggggcggc agacgacttg gttagtggtc aatcgacctt catggtcgaa
    45061 atgatggagg ccaataaggc agtccgtctg gcgacagatc ggtcactcat cctctttgat
    45121 gagttggggc ggggaacggc cacctatgac ggtatggccc tggcccagtc tatcatcgag
    45181 tacatccacg acaagattgg agccaagact ctctttgcta cccactacca tgagttgaca
    45241 gaccttagcc agaccttgga acacctagag aatgtccatg tatcgacctt ggagaaggac
    45301 ggtcaagtca ccttcctcca caagattgcc caaggtccag ctgacaagtc ctacgggatt
    45361 catgtggcta agattgcggg aatgccagag gaactattgc agcgggcgga caggattttg
    45421 cagacgcttg aaaaccaagc tcctactgca ccaactaccc cagctccgat agtcgtggaa
    45481 gagccgtctg gtcaaatcga cctcttcgca gacacgcctt ctcacccagt cctcgatgaa
    45541 ctggcaaaac tggatatcta caatatgacc cctatggaag tcatgatggc agtggcggag
    45601 ttgaagaaaa aaatataaga caatgaaagt caggacaatt gttctggctt ttttgccgtt
    45661 tagtcagttt aggtgttatt ctcggaacca ttatttctct cataacctct tctattgtcg
    45721 gacaaggaac ttatctacct cttctggttt atctttacct tggtatacgc tttgctattt
    45781 ttcctaaacc atcggaaaat ggagcggcaa atcaaggaca ttaacggtcg tttgtagtat
    45841 aatagcacta tcattttgtt ggagtgtttt atgaaaatta ttttgacgat tgtctatagc
    45901 ttgggagtgg tgtggaatat cctctttctt gcggtaaatg ttggcagaat tccagtcatc
    45961 aacttgcttt ggcctgctgt gattttagtg gcctgtgcta ttaacctgta ctttttatgg
    46021 actagaaaac aagagtcaga ataggctctt tttttgatcc ataaatattt tttatggctg
    46081 gctataaata atttaaaata tacgaaaaag agagagcatg ataagatagt gtctatacat
    46141 tacgaaagga gggcaggatg gggaaaccga ttattggcat caccagtaat gaaaagcctg
    46201 tcgcagaggg gattccgatt atgaatttat cgttgtctcg taactttgca gatggaatca
    46261 agcgagcggg gggccttcct ttttatatcc ccttgtctgg tgaaacggat gttgtcgact
    46321 atgtgtcggc gattgataag ttattactga cgggtggtca acatgtatta cctcagtttt
    46381 acggtcaaga aaaactcatc gaaagcgatg actatttcaa ggaacgtgac gaatttgaac
    46441 tgaaattggt ccacgaagcc ttgagacagg aaaaaccgat tttggggatt tgcaggggga
    46501 tgcagctttt caatgttgca cgtggaggaa ctctcaaaca agcggtagag gggcatcggt
    46561 ccttgccttt tgatctgatt caccaggtag aatgtaaaga ggcaacgcct ctggctagta
    46621 tttttggtca gagagtagct gtcaattcta ttcacagcca ggcgatagag aatctgggtc
    46681 aggggctaga agcgattgtg cattctgtgg atgatggtat tattgaggct gtccgatcaa
    46741 tggatgagac acgttttctg ggtgtccagt ggcatccgga acttctgatt gatcaaagtg
    46801 gcggcaatca gaagctattt gattattttg tgcagacctt gtaggtctga cttgcttata
    46861 caaaaggaga attactatga aattaagaac tattctatca ctggctggta ttacagctct
    46921 tgcgacagtt gctttggcag cttgctcgtc tacctcaaac tcatcaaaca ctagttcttc
    46981 agaagcggat aaaaaagaaa ccatcgtaat gggaactgtt gggactacca agcctttttc
    47041 ttatcgagat gagaatggag aattgacagg gtttgatatt gaattggctc gagctatctt
    47101 tgatggttct gaccagtatg aattgacctt tgaaacaaca gatggtagcg gtcaattccc
    47161 tggcttggat tcaggacgct ttgatttgtt agggaataat acttcctata caaaagagcg
    47221 tggtgaaaaa tacctctatt cttatccaac ggcctctaca ccatccgttt tagctgtgcc
    47281 aaaagatacg gatattcagt cttatgatga tattggtggt cattctacac aggtagttac
    47341 agggacaaca acagctgctc agctagagga atacaatact cagcatacgg ataaaccagt
    47401 cgacatcaac tgtactggtg aaaatatcac acagattttg accaatataa atgatggcaa
    47461 gtatgatttt aaaatttttg atgcccctac ggttaatgct attattgcag atcaaaattt
    47521 gacaaatctt aaaacaatcg agcttgaagc agcagaaaaa ccgtttattt actatgtatt
    47581 ttcttcggac aatacggctc tacaaagctt tgtcaacgac cgtatcaagg ctttgaacga
    47641 agatgggacc attgcgaaat tgacagagca attcttcgga aaagactatg ctccaacggc
    47701 ggaggaacta gctgttccta atgaatagga gtcgatagta ttgtgacagg agggagcagt
    47761 ccaagtgggc tgtttctttt tcattgtctt tttataatca ttaagattac ttatcacagc
    47821 cataaaatat atataaggtt gatgtgggtg gttacatgat aaaatagagt atacggcaga
    47881 aaatctgtca tttttaaatg tttggagaag agtatgattg gtgtattagg aataacagga
    47941 ccggcatggt atcaaggttt tttagagtgg ttaccggatg gtaagctatt tagtattagg
    48001 gctgtttttg atgccctacc ctccattatt gaacgtttac cgattacgat tggcttgacg
    48061 ctcgcaggtg ctttttttgg tttgatattg ggcttggttt ttgcgattgt aaagattaat
    48121 cgtgtgcgat tcttatatcc cgttcaggca gtttttgtta gttttttgcg aggcactcct
    48181 atcttggtgc agctgatgtt gacctactac ggaattccat tggttcttag agggctcaat
    48241 caggaatatg gtcttgattg gaatatcaat gctattcctg cagctgtctt tgcgattaca
    48301 gcctttgcat ttaatgaggc agcctatatg agtgagtcta ttcgtgctgc gattcaggct
    48361 gttagtccag gggagattga ggcggctcgt tccttaggta tgacttctgg ccaggtttat
    48421 cgtcggatca tcattcctaa tgcggctgtg attgcaactc cgtcgttgat taacaccttg
    48481 attggtctga ctaaaggaac ttccctagcc ttcagtgcgg gtatcgttga ggtctatgca
    48541 caggcacaga ttcaaggcgg ggcagactat cgctactttg agcggtacat ttctgtctct
    48601 ttggtctact gggcgctctc tgttctgatt gagtctttcg gccgtttcct tgaaaataaa
    48661 atggctattc aggcccctga atcagtagat atttctggag ttggaggtga tcgctaatga
    48721 ttaaaattcg caatttagtc aaggaatttt caggacaaaa agtactagat ggaatcgata
    48781 tggacattga aaaagggcag gttattgccc ttgttggacc atctggagca ggaaaatcaa
    48841 ccattttgcg tagtctgaac tatctggagc aggcagatgc ggggagtatc gaaattgatg
    48901 gttttacagt tgattttacc aatatttcaa aagaggagat attgacctta cgccgcaaac
    48961 tggctatggt ctttcagcag tttaatctgt ttgaacgaaa gacagctctt gataacgtca
    49021 aggaagggct taaaattgtc aagcggatgg atgacgcatc tgcaaccgcc cttgccaaag
    49081 aagaattgag aaaggtcggt ctgtcagaac gtgagaacca ttatccaaga catctgtcag
    49141 gtggtcaaaa gcaacgtgtg gctcttgcac gcgctcttgc catgcaacca gacattttgc
    49201 tactagatga gccgacttcg gcgcttgatc cagagttggt tggagaagta gaacgttcca
    49261 ttgccgatgc ggctaagaca ggacaaacga tgattttagt tagtcatgac atgaatttca
    49321 tttaccaagt ggcggataag gtattatttc tagaaaacgg ccgtatcctg gagcagggga
    49381 cacctgagga gcttttccat catcctaaag aagaacggac tagagaattt ttcgcctctt
    49441 attcaaatcc tcaattttaa ataggcatgt ggtgacccgc cctagcctat tttcaataca
    49501 tagagccagc cctgttagag ggctgttttt taggttttct gatagagaag ttagaccttt
    49561 gcccaatctg ttgtcatatt tcaatctagt aattcatcct tgtcgagtgg tgaaatttgg
    49621 cacagttgtt ttccaattga tagaattttc aagacctata tatagtgcat ggataggttg
    49681 tttaatattt ttttacacaa gatattgatt tttgttttgt gaagtgctac actaatagaa
    49741 gtgaagaatt ttggaggttt gtcagatgaa tgtgcaagaa aatgtcttac cgagtataga
    49801 attattagtt ttaaaacgtg atggacggac agtatccttt gaccaggata agattttttc
    49861 tgctcttcgg cgggcaaacc aagaattaga acatcctgtt tcagaggcag gtttaaaaat
    49921 tgtattagaa gctgttttgg ctgaaattgg tcgccgattt gaaaaagatg ttcaaattta
    49981 cgaaattcaa acggtcgttg aacaggaatt gttaaaggct catttatatg atttggcgga
    50041 gctttatatt caataccgaa cacgccgtga ttttcgccgt catcaggcta ccgatattaa
    50101 ttttgagatt cataagctgt tgagcaagga ccagtctgtg gtcaatgaaa atgccaataa
    50161 ggacgcggat gtgttcaata ctcaacggga cctgacagcg gggattgtcg gcaagtcaat
    50221 cggtcttaaa ttattaccag ctcatgtagc caatgctcat caaaagggtg acatccatta
    50281 tcatgatttg gattacagcc cttatacacc aatgaccaac tgttgtttga ttgatttcaa
    50341 aggaatgttg gcacaaggct tcaagatagg aaatgccgat gtagaaagtc cgaagtctat
    50401 ccaaacagct acggctcaga tttcacaaat cattgccaat gtggcttcta gtcaatatgg
    50461 tggttgctca gctgaccgta tcgatgaagt tttggcgcct tatgcggaac tcaattacca
    50521 aaagcatttg aaagacgcca aggattgggt attgccagac aagcaagaag aatatgcgcg
    50581 tgcaaaaacg caaaaagaca tctatgatgc tatgcagtct ttggagtatg aaatcaacac
    50641 cctctttact tccaatggac aaacaccctt tacctcactc ggttttgggt tagggacatc
    50701 atggtttgag cgagaaattc aaaaagcgat tttgaacatt cgtatcaagg gtctagggcg
    50761 agaaggtcgg acagctatct ttcctaagtt gattttcacc gttaagcgtg gattgaactt
    50821 ggagccagat tctccaaact atgacatcaa gcaattggcg attgaatgtg cgaccaagcg
    50881 tatgtatcca gatatgcttt cttatgacaa gattgttgag ttgacaggtt ctttcaaggt
    50941 gccaatgggc tgccgttctt tccttcaagg ttggaaggat gaaaatgggc aggatgtgac
    51001 atctggtcgg atgaacttgg gtgtcgtaac cgtcaatttg ccacggattg ccatggagtc
    51061 tgctggagat aaggataagt tctgggaaat ctttgtagaa cgtatggcca ttgctaagga
    51121 tgccttggtt taccgcgtgg agcgggtcaa ggaggcgaca cctgccaatg caccaatttt
    51181 gtatcagtat ggggcattcg gcaaacgctt ggctaagaca gacgcagtag atgaagtctt
    51241 taaaaatcgt cgagcaaccg tatctttggg gtacattggt ttgtatgaag tcgcaacagt
    51301 attttacggt ggcgaatggg agaccaaccc agaggcaaaa gatttcacgg tggatattat
    51361 caagaaaatg aaagccttgg ttgaagattg gtcggatgaa tatggttatc atttctcagt
    51421 ttattcgaca ccatctgaaa gcctgacaga ccgcttctgc cgtatggata cagagaaatt
    51481 tggcatcgtt aaggacatta cggataaaga atattacact aacagtttcc attatgatgt
    51541 acgcaaaaat ccaacaccgt ttgaaaaact ggactttgag aaaatttatc ctgctgtagg
    51601 tgccagtggt ggatttatcc attactgtga ataccctgtg ctgcaacaaa atccaaaggc
    51661 cttggaagct gtttgggact atgcttatga ccgtgttggt tacttgggaa ccaatactcc
    51721 aattgaccat tgttacgctt gcgattttga aggtgatttt gagccgacgg agcgtggctt
    51781 caagtgtcca aattgcggga atagcgatcc taagtcggtg gatgttgtta aacgaacttg
    51841 tggttatcta ggcaatccac aggcccgtcc gatggtaaaa ggccgtcaca aggaaatttc
    51901 ggcgcgtgtg aaacacatga atgggtcgag cttataagta ataaacagac tatgtagagc
    51961 ttccacagtt ccagttagct gtggaagttt attataaaca aataagagat aaaaaaagag
    52021 gagaaaacaa tgggaaaata tcaattagac gataagggga aggccctggt gacacgctat
    52081 cacgagaaga attctaatgt gaagcaggat aaaaaagcgc gtgtgcagga attgctcaaa
    52141 caagcaaaaa ataagaaaaa atagtatgga ctatacagat attagcattc tccataccaa
    52201 tgatatgcat tcctacatgg agaattttcc aaagaaggcg caactgattg cgaacattcg
    52261 ggctaggaat gaaaaagagg gagttccgac ctttgtcttt gacagcggcg atctcttttc
    52321 gggtaatatc tttttcaata tgtaccgagg agtcaaggaa atcgagctaa tgaatcaaat
    52381 tggttgtcag gctatgacct tgggcaatca tgagtttgac cacggagatg agttgattag
    52441 tcgcttggat gactatgccc agtttccaat tgtgtcctcc aatctccgct atcaggatgt
    52501 ggaggtggca gatgagttgg tgccgcttga ggatgtcctt gaatttgata tgaatgggaa
    52561 aaacctctat gtcctcggtt tgactacctt ggaaacccag gaggttgcgt caccgtcgga
    52621 gaatgtgatt tttgaagacc ataaacaagc tctgcggcga ttggtggatc agattgtgag
    52681 tcagaatcca tcggctcatc tggtcttgct cagtcatctg ggatacgatg aggatgtgca
    52741 tttggccaag gatttccctg agcttaacgt gattttgggc ggacataccc ataccattct
    52801 aaaacaacct agtcagattg gcggagttag tatctgccaa gctggtcagt atggtcgctt
    52861 tgtcggtcat ctatccctgc ggtgttttgc agatggacgg cacgaggttc aggcttatga
    52921 cttgattgag gtggagaaac tgacccaaga agacagggcg gtcaaggcta ttatcgacca
    52981 gatgaaatcg gaacgtgacg cagctttttc caaaccgata gcgattttac ctcaggcact
    53041 agacggagag cgagacagca ttcgtcaagg gttatcaact ctggcccctg ttatttgtca
    53101 ggctttattt gaacaggcga gtcaactagg tttgcaggcg gatggagcgg tcatcaacgg
    53161 cttcggtatt cgagcttcct tgtcggcggg accaatcttt tattcggact tggtcaaggt
    53221 cttgcccttt tccaagcgag tactcttagt tcgtatcaag ggcagagact tactctccag
    53281 tctccagaca ggcctccacc ctcagatgtg gggcattgtc caaagcggtg aggatctttt
    53341 ggtcaatggc agagcagtcg agtcagataa ggactatcag attgtgacca attcttttgt
    53401 ttggagcggt aaggacaact atgatgattt ccacaaggca gaaattgtgc aggacttggg
    53461 actggatata gacatcatca gtgaattttt caagagacaa tatggaggct agaatggaac
    53521 taagaagacc gactatagcg gataaggaaa cgattttgga aatgctggca gagtttgaag
    53581 cggcaggctc agctatggac ggaggcttta tctccaaaga tgtggacttt gaagaatgga
    53641 tttttagaaa caaagactat gaggcaggtc tcaatctgcc agacggtttt gttccttcca
    53701 ttcaatatgt gtcatttgac cagactggtc gagcccttgg ttttctcagt ctacgcctac
    53761 ggctcaatga tttcctgctc aataaaggcg gtcatatcgg atattccatt cgtccgaccg
    53821 agcgaggaaa gggctatgcc aaggaccagc tgcgacttgg tttacaggaa gccgcaacca
    53881 aaaatatttc caaggtcctt gtgacttgct cgacagacaa tcaagccagc cgtcgcacga
    53941 ttgtggcttg tggtggcgtt ttggaagatg ttcgagacgg agtagaaaga tactggattg
    54001 aggattaatg ggagtgacaa actgctgaat caaaacagga aaggaattga gattggttcc
    54061 gcactaagct tgctgggatc aatccgagct aaagcattgg aagtaccatt tatggataaa
    54121 aacagtctgt gtactggtaa aacggaattc atccaaacga tcagatttca tttgcttatg
    54181 ctcaggtatt tgaaatgaat aatcctaaac cacaagaatg gaagagcgag gaacttagcc
    54241 aagggcgaat aatggactac aaagccttta actttgtgga tggagaagga gtccgttgct
    54301 ctctatatgt tagtggctgt ctgtttcact gtgagggctg ctacaatgtt gcaacctggt
    54361 cttttaaagc agggattccc tataccaagg agcttgaaga ccgcatttta gcagacttgg
    54421 gccagcccta tgtgcagggc ttaactctct tgggtggcga gccttttctg aacacaggca
    54481 cggtcctccc actcgtaaag cgaattcgga aagaactgcc agaaaaggac atttggtctt
    54541 ggacaggcta cacatgggaa gaaatgatgt tagaaactcc ggataagtta gagttactat
    54601 cctatttaga tattctagtt gacggccgtt atgaccgtac caagcgcaat cttatgctgc
    54661 aatttcgagg ctcttccaac caacgcatca tcgatgtaca gaaatcatta caggaaaaac
    54721 gtgttgtcct gtgggataag ttaaaaaaat agcccttcac aatgtgaaag ggttcttttt
    54781 gaccaaattt tgaccaaatt cactcaaaaa acgagaaaaa gccttgataa atcaagactt
    54841 tcctcatatc aaccaatcct ccctgtaggg atcgaaccta cgacccacgg attaagagtc
    54901 cgctgctctg ccagctgagc taaggaaggt aaaaaataaa aatgctgtat tggtaccggt
    54961 tatccatgga ttgtattgat cccgcacgct aaaagcaggt gggtgacgcg tttctaactt
    55021 agttgcttct gggtgtgcca gcttgcatac tgttagagga tctttgtctc cctagtaata
    55081 caaaaatagt cggtcaacac ataggtatga attcgtatac cacagcagtt cttatttatg
    55141 atttaataat accacttttt tttgaaaaat caagagaaaa atggcaaaaa tgtaagcggt
    55201 gtcaaagcgt gtgtacccaa aaaccagttc tatcaaggat tttggcgtct attttttttc
    55261 aaatttttta tcttttcttg tgtcatgccc aaggcacgct cgtattttcc agtgtcgttg
    55321 gcggtgaagt agtccgcagc tagtaactta tcgggcaggt attgctggtc cacccatttt
    55381 tcaggatagg catgcggata ctggtagcca atagcatttc ccagctcctt gctgccagca
    55441 tagtggccat cccgaagatg atttggaatg ggcagatggc cgtttttccg caaatcagcc
    55501 agagctgcat ccatagccag ataggcagaa ttggacttgg gagaaagagc caaatcaatc
    55561 accacattgg caatcaaaat gcgtgcctct ggaaagccaa ttttctgggc ggcttcaagg
    55621 gccgtcaccg tatgaatctg agcctctgga ttggccaagc cgatgtcttc gtaggcaatg
    55681 accgtcaagc gacgggccag actaggcaga tcttcggcct caatcaaacg agcggcgtag
    55741 tggaggctgg cattgacatc gctacctcga atggattttt gtagggcgga gaggatatcg
    55801 tagtgggcat cgccgttctt gtccatgctg atgtaggact tttgcaggct attttccacg
    55861 gcgtccagat caatgtggcg gctaccgtcg tcactttcct tggtcgaaag cacagccagt
    55921 tctagagaat tgtaagcggc acgaaggtca ccgtttgtcg catttgccag aaaatccagg
    55981 gcctcaggtt caatggtgac gggaaaatca aaaccacgtt cgctgtctgt cagagccagt
    56041 tccaaggcct gtcggatgtg actggttttc aaaggttgta attcaaaaat ctgcacccga
    56101 ctgcgaatgg caggcaaaat tgagaagaag ggattttccg tcgttgctcc aatcatgatg
    56161 atattgccgt tttccaaaag aggaagcagg aagtcctgtt tagtcttgtt gaggcggtgg
    56221 atttcatcga gcatgaggac caggccacca gaaaatttag cttcttcagc gatttcctgc
    56281 aggcgttttt ggttgtctgt cgtggcatta aaggttcgaa aggcatactt ggtcgtacca
    56341 gcaatggcgg acgcaatcga ggtcttacca atccctggcg gaccgtagag aatcatggac
    56401 gacagcatat tggcatcaat catgcgacgg ataatctttc caggaccgac caggtgttcc
    56461 tgaccgatga cttcatcaat ggatttgggc cgcatacgaa gggcgagatt ggctggcata
    56521 ggcttccttt cgtttctttt gttagctgat tatggtacac ttattataac acaaaatcga
    56581 aagaggactg acatggcaaa acatggattt ttagatgtgc tggaagcggc actggacaaa
    56641 cattttacct acgattacga gctcaactgg gacaagaaaa accacgcagt tgagttggct
    56701 tttatcttgg aagcgcaaaa tgtggctggg attgaaacgg tggacgacga aggtaatgcg
    56761 tcttctgagg atattttcct agaggagtat gtccttttct acaaccagga caagtctcgc
    56821 tttgacgagg aagactacct ggtcgctctg ccatttgacg ccaagaaggg cttttcagcg
    56881 gagtttctga cctacttcgc aggctttctc aaagataccg ccgaccaagg tttagatgac
    56941 ctcatggatt ttttggtgga cccagaagcc caagattttg cgatttcttg ggacggagag
    57001 gcttttgaaa aaggcagagc agacttgttg gagggagaat tttacccata tccgaggtat
    57061 tgagttggtt catgaaatgt tactggctcc aaaacctttt gaaatgatga agtctggtca
    57121 gaagaccatt gagctacgac tttatgacga gaagcgcaag catatacaaa ttggagatcg
    57181 catccgtttt tattgtacag aaaatcagac acaaacgatc gaggtgcaag tattagatct
    57241 tcacatattt gataattttg ctcagttata caaagaactg gatttattgt cttgtgggta
    57301 tacgcaaagc agtatcagag gcgcgaaacc cgaagatatg gaagattact attcacgaga
    57361 acaattagaa cagtacggtg cagttggtat agaattgagg gtaatagatt ctatttgaca
    57421 ttccgttttc tttcttgctt gactgcaggc gaaacttttg ctattataaa aatagaaaag
    57481 gcgttgagtt ccagctcaac gccctgacag gaccataacg atggttcagt tcgttagatt
    57541 aaataaccgt cagaactgtg tcaaagtttg ctgacggtta ttttttatct cgaatagcta
    57601 aaatgatgac gattatcaag ctcagcattt caacaagtag acttgataaa tataaatcca
    57661 gagttctccg acgtgagagt tttgaaacga tatttttcaa aactcgagct agtgcgtgcc
    57721 ctagacaaat cttataggat ttgtcgttgc cagatgagta agtttactta ccatctggca
    57781 tgagctagcc tttctagcag atttgctgtc cactttcatg ggcatcacct ccgttcagaa
    57841 acttggaacc accgtctcaa actttcctac acatgtttat tcgcagtgcg attagaaaat
    57901 tttcgaaaaa gtattattag ggctccatcg cgtaacatta acttgtaaaa taggctagaa
    57961 atcggtacaa taatacagtg attgacgcat agaaaagaaa aggaacaaca aacatgaact
    58021 catggcaaga attaacgatt cacgtgcatc gtgatgcaga agaagcagtt tcaaatctca
    58081 tgattgaaac gggcagtcag ggggtggcta ttagcgactc ggctgactat gtggggcagg
    58141 aggaccgttt tggtgaactc tatcccgagg tggaacagtc ggatatgatt gtgattacgg
    58201 cctattatcc tgatactgta gatattgagg aagttaaggc agacttggcg actcgcttgg
    58261 cggatttgac aggctttggc ttggaaacag gtcaggtcag cctagaaagt caggaattgg
    58321 cagaggaaga ctgggcggac aactggaaga aatactatga gccagcccgc attagccacg
    58381 atttgaccat tgtaccgtct tggaccgact atgaggcgac agctggggag aagattatcc
    58441 gcttggatcc aggcatggct ttcggtacag gaacccaccc gacgaccaag atgagcctct
    58501 ttgctctttc tcaggtcttg cgtggtggcg agacagtcat tgatgtcggc acaggttcag
    58561 gtgtcctttc tattgctagc tccctcctag gtgccaagga gatttatgcc tatgacttgg
    58621 atgaagttgc ggtgcgtgtg gctcaggaaa atattgacct caacgctaat actagcaata
    58681 ttcatgttgc agcgggcgat ttgcttcgtg gcgtagatat taaagcagaa gttatcgttg
    58741 ccaacatctt ggcggacatt ctcatccatc tgaccgagga tgcctaccgt ctggtcaagg
    58801 acgaaggcta cctgattatg agcggcatca tcgcggataa gtgggacatg gtgcgagcat
    58861 ctgcggaggc ggctggtttc ttccttgaga cccatatgat tcagggcgag tggaattgct
    58921 gtgtctttaa gaagacggct gaccgctcag gtgtgattgg aggctaggat gcagcagtat
    58981 tttatcaatg gcagagcacc gcagggcgtg ttccagataa gcgataagga caccgccaag
    59041 catatgtttt ctgtcatgcg cttgcaggca gatgaacaga ttgtcctagt ctttgacgac
    59101 gggattaaac gcttggcgcg cgtggtagat agccagaacc aatctgttga aatcatcgaa
    59161 gaactgtcag acaatgtcga attacctgtt tccgtcacta tagccatggg ctttcccaag
    59221 ggagataagc tcgaatttgt cgcccaaaag gcaacggagt taggcatggc agccctctgg
    59281 gcctttccag cggactggtc cgtggtcaaa tgggacggta aaaaattagg caaaaaagca
    59341 gaaaagttag aaaaaattgc ccaaggcgca gccgagcaga gcaaacgcaa tcgcattcca
    59401 gctgttcgtt tatttgagaa aaaagtggat ttcctagctc aactagcagg ctttgaccag
    59461 attatcttag cctacgaaga ggccgcaaaa gagggcgaac aagccaacct ggtgaaaatc
    59521 ttgtctggct tagaaatcgg ccaatcagtc cttgtcatcg tcggtccaga aggtggcgtg
    59581 tcgcctgagg aagtagccgc atttgaagga gcaggagcgg tcaagacagg tcttggtcct
    59641 cgtatcttgc gagcagaaac ggctcccctc tacgcccttt ctgctattag ttacgcgacg
    59701 gaattgttga ggtaaggaga atcgggagat gagtgaatta gaacaaaagg aacaccgaga
    59761 attgattgtt tcggatgatt gctttgctct cattcagaaa atgagtgagt tggattcatc
    59821 agcatatcag agatatgaaa ttacgaatcc gaatattttg gctcatgttg aaaagatagt
    59881 tcaaccagca cttaaacatc atcttaagaa agccggacag cctaatattc ctaagggatt
    59941 gtttaaagtg gatttgccga taggcgattt gcacaaaatc aaaggggaaa cggataagta
    60001 tcgtgctttt gttaaaggtg cggaaggaaa atttaaggct catgcaatac taactcctgc
    60061 tcaagttgaa attgcaaaag gagcaaaagc tgcagctgct gtttcaggag ctatgcaggt
    60121 cgctgctatg gtagttggtc agtattatat ggctgaaatc aatggtcagc ttgattcaat
    60181 gaaagaaacg ttgaacgatg ttaaggattt tcaagtaagg gaatttaaag ggactgttcg
    60241 cgctttgata gttaatattt ctcaactatc aaaattttca gcagactatg tcgaaaatgc
    60301 tgaacttgca agactaaagt tgatggagtg tgctaacttc cgtgctgaag taaccaagtt
    60361 actagaacag gtcaatctag ctattgaaga gagtatcact aagggaaaag gattatcttt
    60421 tgaaaagtat gaacagttaa ttgataagtt aagtactaat ttattctatc aacaaacctt
    60481 gttgagatta ttagcggaac ttagtaagct agatctagtc ttctcgcaag gagtggcgtc
    60541 agaggatagt agttactatg tgtatgagta ctatctaaac aattgtaata gtctaggtga
    60601 aaagatagcc aattggcaca aagataagat aaaatttttc caaattgaca ctaagcgcaa
    60661 tagacgtacc caagagggtc ctatgaaagt agttgcagat attgcaaaag agttggataa
    60721 gaatgcccat gttgctgctg gagtaggctt tcttggaggc cttgttggtg cagcagcagg
    60781 ttttggaatt ggtaaagcgg ctggagagct aaataaggcc aatcgttaca ttgatgttcg
    60841 aaaacggaca gttaagctaa tttcagaaca agaaactgtt tcctatcaat ctagtaagcg
    60901 attgtctgtt aacaattatg gggagaatgt gaaattgttg gttgaagatg gaaaagttta
    60961 ttatctattg ccagatgata gtgaatcata aaggaggatc atgttcgcat taggaaccat
    61021 tatcaatacg gtggccattg ccctagcagg tttgctagga tcttggtttg gccatttact
    61081 gaaagaacgg catcagtcag gactaacggt ggcgagcggg ctagctgttt tatttctagg
    61141 aatttcaggt agtttagaag gactgctgac agtcgttgat ggtcaactca aaagtcaaaa
    61201 tagcatgctt ttggttctta gtctagccct ggggaccttg attggggaag tactgcatat
    61261 cgaaggttgg tttgagcgct tgggtatctg gctccgagaa aggtcaggaa acagccaaga
    61321 tggtcaattc ttagatgctt cctgacagct tctttgaccg tctgtatcgg tgctatggcc
    61381 atactaggag ccatccaaga tggattgacg ggtgattatc gcttgttggc cgttaagagt
    61441 attctggact ttattatcat tttgattatg acctcgagtt tggacaaggg agcaggcttt
    61501 tcagcagtac ccgtttttct ctttcaaggc tctgtgactc ttttggcccg cctgattgaa
    61561 cctttgatga cggatcaggc ccttgccaat ctctccttta tcggctccgc cctcatcttc
    61621 tgtgtcggtg tcaatatcat ctgggacaaa aagattcgtg tggccaatat gttgccagcc
    61681 atcgtgattg cagttatttg gtcatatttt acatgatctg gtttacattt tcatacctga
    61741 ttagttgttt tttaaaaatc atattgatat actattattg taataaagtg aatgatttat
    61801 catctaaaga gagaaaatag tctcgaacac acgttagcaa atagtgaatg tatcgctctg
    61861 aacgattcga ataaacagga tcgtgatgga ttcgttcata gctcaatggt cgttgcaatt
    61921 agcgaagatt gaggagatat gtgatggaga ttgcccatga aaagattgag tacccctatt
    61981 tttgctgctc ttggattttt cttgatagtc tttgttgctc ttaatcaatt cggtagagtt
    62041 ccagggacta gctatcagat tatctcggaa ttggttttgt ataatcaagc aacagagcag
    62101 acctatgagt ttgaaaatca tgaagtgaga aaggctttta ttcgtcaact atcgagtacg
    62161 tctagtcgag gttttcaagc gtctgatagt cgtacttctc tcgaaacaac ctatcatgaa
    62221 accattatga gcgaaaatct gatttcttcg atttatggtg gagaagaagg tagaattatg
    62281 gagtttagtc gtcctagaaa atcgactttg gggaatctcg tctttgcttc agaaagcgtt
    62341 tcttaccttg tttttgcaag gaagtatgga aacattcgct tagaaacaac gatcctttgt
    62401 caggagttta gagtagaaca gcggttggcg aaccaatcaa actgggtaga tttgggaacc
    62461 tatacagtaa aaagtcccaa agaaacccgc tatgtaccga tttattggga gtagcgatat
    62521 aaagataaaa atagctcgag tacgtcttcg agctattttt atatgctttg attaatttat
    62581 ctagagagtg tatgagggta aaaaagagct tgaaaaaaca agctcttgat tattttaatg
    62641 ttcttttttc ttagtccatg cgccgacaaa accaatcaaa gtgagaccga gcagactaag
    62701 catagaagta gcttctcctg ttgcagggag tgtcttggtg ctcgcttgtg aatgtggact
    62761 agccaattta tgttgtggag caggagcagg tggatttacc gctggaagag taattcgttg
    62821 accagattcg agtacaatat cttgacctgt gttaccttga tctgactgct gttcatctgg
    62881 agtcgctact tttggctcag tgtagacaaa tttgaattcg ccaaatcctt cgttttcagc
    62941 tgaggcttgg aggtaaacca gcccttcttg attagctacc aattcttttg cacgatctgc
    63001 tgtcaagaag cggaggtcga gtcccttgat gctatctgta aaggtccagt tattgtctgc
    63061 tgtagggttg ataactttct cagcgataat ataattgatg atggcttggc ggttttccag
    63121 attgagcaag taattgacag aagcttcgcg gaccccaggg aaagtaccgt ttgcacggta
    63181 gttgttggtc accacgatga attcttgatc agctgtcaca tcttgcccat tgtattgcag
    63241 attccgaact ctgctggcag tttcgttgac taccttaccg ttacgatcgt atttatttgg
    63301 ttgcgtaata tcgtactgat aagttactcc gtcaataacg tcaaaattgt aagtgcggaa
    63361 atctgtgttg actaggtttt gtggctctgt tgagcttggg tcaacttggt tgaattgccc
    63421 tgcggacatt tccaaccatt ctttgagttg ggcaccgttg acttttaaga tggctacaac
    63481 gttgtcgtag aggtaaaggt ctgctacgtt cttgatggcg attgggccag caggaatgtc
    63541 tgtataggct gaagcatcgc ctcgagtacc agccttaaat ggtgcagccg cagataggat
    63601 tggtagatta gcttctgaag tacccgccaa ctgttgtttt gcataccaaa tctgtgcatt
    63661 gttaacaatc tgtacagaag gatcatcttg aacaagggca aagaagctgt tgattggagc
    63721 agttgtctca ccgacttgtt ggcgcacata gttgatggtt tcgttatggg cttctttagc
    63781 aaggtcgatg atgcgaccat ctgctacaga tgattttgta tcgattttac gaatagccgc
    63841 tttgctagaa gtagttgtcc atttgccatc tttaaagacc agattgaggt cgatgacgcc
    63901 gaggtgatca ccgtatttac ccgccatggt gacaggagta ccgttgattt taccatttgt
    63961 atcatccacg ccagagtatt tagcatagaa gcttggtttt tcggcagttc ctgggaattc
    64021 tgcgtgggag tgacctgtga taaccgcatc aactcctgac aagctggcga tttggtaacc
    64081 gacattttcc tcgccgactt catattggtc atccccgata cctgagtggg aaaggaccag
    64141 aacgatgtct gcgccatttt ctcgcatggt tgggataata tcgcgaacgg cttcaactgc
    64201 atcacgaaca atcacttttc cttctagata agctttgtcc cagttaagaa tttgaggggg
    64261 aacaatccct gtaacaccga catttagtgt aaccttttta ccctcggtgt ctgtgaatgt
    64321 ctttttcaca atggtataag gggtgtagag aaagtctttt gtagtcggat cgaggacatt
    64381 agcattgact agaggcatgt tggctgtacg aattactttt tcgaggtagg caaggccgta
    64441 gttaaattcg tggttgccaa gtgtgccgac atcaaatccc agagtctcta aggcggcgta
    64501 cataggatgt tgttcacctt cttcaatagg atcaacgata gacttgtaat ttccaagagg
    64561 agtaccttga atggtgtctc cattatctac caatacgaca tttggatttt ctttttttgc
    64621 ctcttcgata agaacagctg ttttggctag acccagtgtt tctacaggtt tatcctggta
    64681 atagtcatag ttgaccaagt tcgtatggag gtcagttgtt gcgaggatac gaacatcaac
    64741 agactggcct tcgacaggag ttgtagtctc agtagccgct gcgcgtgttg ctgtaacagg
    64801 gctagctgtt gtgtcgttaa ctggagtatt gctggtttct acaacaggag cagcttcttc
    64861 tgttactggt gtaacagact ggtcagtggt ttctgtagtt ggcgtgacag cctcaccggc
    64921 tgtagtttcg gtagttgtcg gtgtggcaac ctcaccagtc gttgcttcgg cttggctggt
    64981 tgctacagag ctactgattt ctgtttcagt cttcaaaaca ggggtatctg tgctggtttc
    65041 ttcagcagag acaagggcgt tgttggtgct agccagtaaa gctaggctga gggttagggc
    65101 acacttacta aaacgaaatt tcatgagaac ctccatttga tataatctca agaatattat
    65161 acatattgaa aacgaaaaca tcaacggttt ctacgaaaac acaaaagaaa aataaagaaa
    65221 aattttcata aaagcaacaa aaaacgaaca atgactaaaa aattgtttgt ctggttttct
    65281 ttgtttatcg tcattttgaa tggtatagta tggatgaatt accgctttcc tttttctttt
    65341 ccagtgaaaa tggtaaaata ataggaaata gttagggaga gaaacaaatg aaggaaatca
    65401 attttacagg agaagaggtt gttgggctga ctgccaccta ccttccagcc gaagatgttg
    65461 cttttgttca gaaggccttg gattttgcga cggaagctca taagctacaa tttcgtaaat
    65521 cgggcgaacc ctatattgta cactcgatcc aggtagcagg tattttagca ggactcaagc
    65581 tggatgctgt gacggttgcc tgtggtttct tacatgatgt ggttgaagat acggatgtaa
    65641 ctttggatga tatggaggct gaatttggtc cagaggttcg ccatattgtc ggcggtgtga
    65701 ccaaactggg taaggttgag tataagtctc atgaggagca gttggctgaa aaccaccgca
    65761 acatgctgat agccatgtcc caagacatgc gggtcattct ggttaaactg gcagaccgcc
    65821 ttcacaacat gcggactctt aagcacttgc gcaaggacaa gcaggaacgt atttcacgtg
    65881 aaacaatgga aatctatgca ccgcttgccc accgtctagg tatttcttct atcaagtggg
    65941 aattggaaga catgtctttc cgctatctca atgaagttga gttctacaag attacgcgca
    66001 tgatgaatga gaagcgccgt gaacgggaag agttggtcaa tgaagttgtt gataagctaa
    66061 gagattatac agaagagcgc catctacatg gtcagattta cggtagaccc aagcatattt
    66121 actctatcta tcgcaaaatg cacgataaga aaaagcggtt tgatcaactc tatgacttga
    66181 ttgccattcg ttgtatcatg gatactccaa gcgatgtcta tgctatgtta gggtatattc
    66241 atgaattgtg gaaacctatg ccaggtcgct tcaaggacta cattgccaac caaaaagcta
    66301 acggctacca gtctattcat accacggttt acggaccgag aggtccaatc gagttccaaa
    66361 ttcggaccat ggaaatgcat caggtggcgg aatacggggt ggcagctcac tgggcttaca
    66421 agcgtggtgg caaggcaact gccagcgaaa aagagttgcg ctggattaac aacctgattg
    66481 aactccaaga aggggctggg gatgcgcaaa cctttgtaaa ttctgtcaag gacgatattt
    66541 ttacagaacg tatttacgtt ttcacgccag atggtgctgt gcgtgagttg ccaaaggatt
    66601 ctgtaccgat tgatttcgcc tatgaaattc ataccaaggt cggcgagcga gcaacaggtg
    66661 ccaaggtcaa cggccgtatg gtgcctttga cgaccaagct taagacaggc gatcaggtgg
    66721 aaatcatcac ctctgccaac tctttcggtc caagtcgtga ctgggtcaac ttggttaaaa
    66781 ctcacaaggc tcgcaataaa atcaagcagt tcttcaagaa tcaggacaag gaattgtctg
    66841 tttcaaaagg gcgtgagatg cttcagaact tactccaaga aaatggctac gttcccaacc
    66901 aatacctgga ccgtcgccac atggatgagg tcctgcaaaa gaccagctat aagaccgacg
    66961 aatccctttt tgcggcagtt ggttttggag aagtttctgc catatcaatc tttaaccgtc
    67021 tgacagagaa agaacgtcga gaagctgagc gggctaaggc caaagctgtc gcggatgaat
    67081 tggtcaatgg tggcgaagtc aaacacgaca acaaagatag tctgaaaatc cgtcacgaag
    67141 gtggcgtggt cattgaagga gcgtcaggct tgctgatacg gattgccaaa tgttgtaatc
    67201 cagtacctgg cgatgaaatt gtcggctaca tcaccaaggg gcgtggggta gctgttcacc
    67261 gtgtagactg tatgaactta aagagtcaag aaaactacga tgttcgcttg attgatgtgg
    67321 actgggagga tgagaattcg accaaggaat acatggcaaa cattgacatt tacggtctca
    67381 accgctcagg actgctcaat gatgttctca aggttttgac caatgccagc aagaacattt
    67441 cttcggtcaa tgcccagcca accaaggata tgaagtttgc gactatccac gtttcctttg
    67501 gtatttccaa cctggcaacc ttgaccagtc tggtggacaa gattaagtcg gtgccagaag
    67561 tttactcagt gaaaaggacc aacggataag atgaaaattg tactacaacg tgtatcacag
    67621 gccagtgtga ccatcgaagg aagtatccat gggcaaattg aacaagggct tttgctccta
    67681 gtcggtgtgg ggccagatga cacacaggaa gacctggact atgctgttcg gaaaattgtc
    67741 aatatgcgga ttttttcaga tgaggcaggc aagatgaaca agtccgttca agatgtggcg
    67801 ggcaagattt tatccatttc ccaatttacc ctcttttcgg acacccaaaa aggtaatcgc
    67861 cctgccttta caggagctgc agctcctgcc ctagccagcc aactctatga tgcttttaac
    67921 caggccttgt cggcctttgt gcctgttgaa gtgggcgttt ttggggcgga tatggcggtt
    67981 agcctggtca atgatgggcc ggtgactatt gttttggata cgaaaaataa gtaaaacttt
    68041 tggcaacggt atgtactcta tttgtgtatt ttgatatact aatattgaaa aataggtatg
    68101 gaggctacta tgaaaaaatt gctcacaaat catttctttt atttgacgat tgcctttctt
    68161 ttgattttag tgatttactt tagtggaatt gacaaacgct ggattatttt ggcaagtttt
    68221 ctttacttca ttccatcgca gattttatac cgtcgtcgcc taagagagcg actccaagaa
    68281 gaccagcccg agtaggctgg ttttttcgac agtattggtt atttgaaaac aatgtgctat
    68341 gataatattg tcataaagtg gtagtttatg ctttgaagtt aggatgaggt gggcagaaat
    68401 atgagaaaat tttttcaaga ccttaatcaa gagtatctgg aaaccagaag ttcagttgga
    68461 gaagattggc tggagtggat tatccagaga aagatgacct ggggtatgcg gtgggggttg
    68521 gtagggattc tcatgtttgt cttgcagcct ttaacctata accctcattt tctattgggc
    68581 tgtttttata taggtttcct actttggttg gtgtatgtat gtgggcaact atttttgcag
    68641 ccagatctat tttccgtaat ttgaaaaaga ggtaactatt tattttagaa gaaaaaatcg
    68701 ctcagcttgt ttaggctggg cgattgcttt tttcgatgaa taattgttga gcgatggctg
    68761 ttgggacggc gagctctttg tccttgtact ggatggtgat ggtctgggca aactggtcga
    68821 aggctgtgac ggttagtaaa tcaccgacag ctaattcatg ttgttccaag tactgaagga
    68881 gttgatagaa gtcatggata cggacaagct ggtagttccc tgtctcagtt agctgtgaca
    68941 gccgtgtctg gtagcgttcg atgagcggtt gtcctgcttg aggaatgctt cccccgtgag
    69001 gacaagtttg aggctgttcc agtagcaggt ctaggcgatt gataaagtga tctgaaacgg
    69061 tgtgttctaa aatctcagct tcttcatgta cttcttctgg agagtagccg agttgctcaa
    69121 ctaagaatac ctcaatcaag cggtgtttgc gatagaggct ggctaccatt tcaagggcag
    69181 tttggctgag gagataacct gccttggcat ccttagaaat gagctcttcg gctaccattt
    69241 ttttaagcat ttcggaaacg gctggtgcgg agaaggccat cttctctgcg atgagtttat
    69301 tggtaatttt ttggtctaat tgacctagtt cataaataca ttttaggtaa tcttctttgt
    69361 ttggtgtcat gagaactcgc tttcttctga aactattata tcaaaaaatt taaaaatcag
    69421 ttgacaaaat gattttttta gattaatcta attatatagt taggtatgct taataaaaat
    69481 aaaaggagaa cctatatgaa gaaaatattg ttcagttttg ctctgctatt gtccctaata
    69541 gggttaggag cttgtcgtcc atcgcaaacc acagaaggct ctagtaagcc aagagtggct
    69601 gtgactactt ccttcctcaa tgacatggtc tatcagttgg cgggtgatga ggtggaacgt
    69661 gatttgctga ttccagcagg agaagaccct catttgtacg tagccaaatc tagtgattta
    69721 tcaaaattac aaaaggctga ccttgttctc tatcacggtt tgcactttga aggtaaaatg
    69781 gttgaagctt tggagaagac cggagtagct gtttctaaaa actttaatgc aaaagacttg
    69841 aatacgatgg atgaggatgg agaagagatt gtcgatccgc acttctggtt ttcaattccc
    69901 ttgtacaagt cagctgtagc tgtagcttca gaggaactac aaaaactctt acctgctaaa
    69961 gctgagatga ttcagaaaaa tgcagaaaaa taccaagcgc agctagatga tttgcatgcc
    70021 tgggtagaaa aagaattaag tgtgattcca aaagaaagtc gctatttggt aacaccacat
    70081 gatgccttta attactttgc tgctagttat gacttcactc tttatgctcc tcaaggcgtg
    70141 agcaccgact cagaagtggc taacagtgac atgattgaga cagttaacct aatcatcgac
    70201 cataatatca aggctatctt tacagagtct acaaccaatc cagaacgcat gaagaaattg
    70261 caagaggcag taaaagccaa aggtggtcag gttgaagttg ttactggcga aggtaaggag
    70321 ttgttctcag attccttggc accagaaggc gaggaagggg atacctttat cgacatgtac
    70381 aaacataatg ttaaactgat ggttaaatat ctgaaatagt acagatcgaa cggacttgct
    70441 attcaatgaa gactagagat attaccgaag atagagttga taggtaccaa tatgagggac
    70501 aatcaattgt ttcgagcaca agcggtatag ggctctttct attggaggtg gtaacgcttg
    70561 ggggaggata ggcgttagta gcaaatcaca ccagttattg aggtccttga ctagaaatac
    70621 cgcttgtaga cctcaatgat cttggttcac agcgggactt tatatatcag tagtctgtgc
    70681 gtgaataggg atttcaaatg agtagtaaaa aggactcggt ctaggtcgag ttctttccgt
    70741 ttttggacca tttttaacag atttataaac atacgattga aggaagcatt ggtgctctgc
    70801 gaaaatcaaa ttcatgtact gatgtgttga aataagcgat gtatagggtt tgccagcaat
    70861 ttgttggtac ggtatcttaa tcttatcgac tttctcagct tggtaggttg gtctggtttt
    70921 gattttcatt gagtatgaat gaggcatatc atttttaaaa ataggaggaa atatgtcagc
    70981 aatcattgaa ttaaaagatg tcaatttagc atatacagcc agccagacca tggctttaga
    71041 agatgtcaat ttggtgattc ccagagggag tcggacggct attgttggac caaatggtgc
    71101 tgggaagtcc agtctattca aggtcatttt ggggttggaa aaacctgaca cagggcaggt
    71161 tcgcttgctt ggtcaggagg caggtctaga gaggttgatt gctcaaaaag tagcctatat
    71221 tccccaatcc agtcaagtaa attggcaatt tcctgcaaca gtctttgaaa ttgttctgat
    71281 gggacgcttc gctcatagta agggaatgtt tcgaagaccg actaaggctg atagacagat
    71341 tgttgagcag gctctagaac ggttaaaaat tgcggattta cgccatcgtc agattgatca
    71401 gctttcaggt gggcagcgtc agcgggtctt tctagcgaga gccctggcac aggaagcaga
    71461 attgtatctt atggatgagc ctctagcagg gattgatcag gcaacagaaa tcatgatcat
    71521 ggatatgctc aaggagttcc aatgtgaagg gaaaacttcc attgtcattc atcatgactt
    71581 aacgacgctg gatgcctact ttgatcacct tgtctggctt cataagcatg tgattgactc
    71641 tggtccgatg gatgagactt tgacaagcga gaattatcag gcgacctacg gcataggaaa
    71701 tgggttgttt ctaggaaatg ccaaaggagg cagtcatgtt tgaggttttc aaagagtatt
    71761 ctttctggac agttgcttta gggaccgtca gcctagctgt agcagccagc actatcggaa
    71821 gtatatctgt tcttactaaa cagagtttgc ttggggatgc tcttgggcat gcttcttatc
    71881 ctggagtgat tgtctctttc atgatttttc agtcgcgaca tcctctctac ttgctgttag
    71941 gagcagtgtt gtcaggctac ctttcttatg tacttgtcca ttggttgcgt cgaaaaggtg
    72001 gtcacagcct agtcaatgcc ctttcattgg tgtctgcgtc tttctttggt ttgggaatgg
    72061 tcttgaaaaa tgctattcag ggaaatgaag cctttgcagg gtcctcccaa gctggattgc
    72121 aaacctatct atttggacag gcagccttta ttcagctgga tgatgtgata ctcatcggaa
    72181 ttatttctct attggctttg gcattgttta ctttctttta tcaagactat aagctatatc
    72241 tgtttgatca aacttttgca agggttattg gtgttcgtgt aaagtacttg cagcagttga
    72301 cgatgttttt gatgatttgc ctaattgcag taggattaaa gttagtcgga gctattttga
    72361 tgagtagttt tttgattgca ccagctgtct ttggcttgat gctgggaaaa tcgtatcata
    72421 agagtctgtt actggcagga atcgttgcaa ttgggtcggc tttcgtaggg acctggatta
    72481 gctctagtgt atctggttta tcgaccggac cgactattat cgtctgtctg acaggtctga
    72541 ccctgtctgc atttgtgtat gttacctatg tcagaaagga gaatggtcgt gtttgaagta
    72601 ttgctcattt tgatggttat tgctagttct tgtggcttgc tcggctcgat actggttgta
    72661 aaaaatcaat cgatgttggc agatgcacta tcgcattctg tcttgcttgg gattgttctg
    72721 ggatttttta ttagtcatag tttggattcg ccattgctaa tagttggtgc cagtctgttt
    72781 gggttattat cggttcttgc cattgaccgt ctgcatagtc gaaagatagc gcatgatgcc
    72841 gcaacaggtc tagttttttc tttctttttt gcggtggcgg ttttgcttat ttctcttttt
    72901 gctcgcaatg ttcacttgga tgtggatctg gttttgcagg gggaagtact ttttgctccg
    72961 ctccatcgta tggatgttct agcctggtca ctccctgtta gtctggtcaa gtcgagtttg
    73021 gcttggttgg ttattgttct attttttgct tgggcttacc atcgtttaca agtgtaccta
    73081 tttgatagca accatgctcg tttgtctggc ttacgaacaa ggattttaga aatggttatt
    73141 ttgattctag tttccttaac tactgttctg gccttcgaag cgattggctc gatgactgtt
    73201 attgttttct tggtggcacc aagtatggct gccctacgtt gggtcaaatc cttttggcaa
    73261 ttgctgcttt tagggcaagg tatcgctatt ctaacggttg tgctaggttt tttagtggcc
    73321 aatcaactgg atttaaccat gtcagggacc tgtgctgttg tcagccttct tgtggtttgt
    73381 tcgagtatta ttctaaaaaa tacttggtca cggtcatctg ataagtgaaa ttgtcagaat
    73441 tttttgttat tatagtcatt aagaaagaca aaggagaaaa tcaatgacac gtttacaaga
    73501 tgatttttac gaatacgtca atggtgaatg ggcaaaaaca gctgtgattc cagacgataa
    73561 gccaagaaca ggtggtttct cagacttggc ggatgagatt gaaaagctca tgattgacac
    73621 gacaaacgct tggttggcgg gagaagatgt cccagaagat agcgttttgc aaaactttgt
    73681 ggcttttcac aaacaagtag ccgactatga aacccgtgac cgtttgggag cagaacctgc
    73741 tcaggccttg attgcagagt acaaggctct caactctttc gaagaattca ccagcaagtt
    73801 ggcagaatat gagttggcag gcaagccaaa cctcatgcct ttcggcgtgg cacctgactt
    73861 tatggacgcg accaccaatg tcctctgggc agattctcta ggcattatcc tcccagatac
    73921 gacctactat gaggaaggtc atgaaaaggg tgcagaatta ctcaaaatct ggcgtgagag
    73981 ccaagaagcc ctcctgccaa aatttggttt cagcgatgag gaaatcaagg acctcttgga
    74041 caagcgtttg gaactagatg ccacgattgc caagtatgtt ctgtcaaatg aagaaggttc
    74101 tgagtacgcc aaactttacc acccgtacga gtgggcagat tttactgcct tggcaccaga
    74161 gttgcctttg gatgatttct tcacagcgat tttaggtcaa acgccagaca agattatcgt
    74221 gcctgaggaa cgtttctggc aggctgccaa ggacatttac agtgcggata attgggagct
    74281 tttgaaggcg accttgattt tgaaagcggc tggcgcctat actgccttcc tgtcggatga
    74341 aattcgcatt ctagcaggag cctacagccg tgctctttct ggtacaccgc aggcgcaaaa
    74401 ccaagaaaaa gcagcctaca acttggcaca aggctacttt aaccaagccc tcggtctttg
    74461 gtatgcagga gaaaaattct caccagaagc taaggccgat gtggaagcca aagttgccaa
    74521 gatgattgag gtttacaaat ctcgtttgga aacggcagac tggttggctc aggaaacgcg
    74581 tgacaaggcc attgtcaaac tcaatgttat caagccttat atcggctatc cagaagcctt
    74641 gccagagcgt tattataaga agattgttga cccaagcaag tccttggtgg aaaatgccat
    74701 cgaactaaac aagattgaca tcgcccatgg ttggagcaag tggaacaagc ctgtggacat
    74761 taaggagtgg ggcatgccag ctcacatggt caacgcctac tataacccac agaagaactt
    74821 gattgtcttc ccagcggcga ttttgcaggc tccgttttac tccttggagc aatcttcgtc
    74881 agccaactac ggcggtatcg gtgccgtcat tgcccacgag atttcccatg cctttgactc
    74941 aaacggtgct tcctttgatg agcatggtag cttgaataac tggtggacag aggaagatta
    75001 tgcagccttt gaggcccgta cccagcaggt tattgatcag tttgagggtc aggattctta
    75061 cggtgccaaa atcaatggta agttgaccgt gtcagaaaac attgctgacc ttggtgggat
    75121 tgcagcggcc ttggaagcag ccaagtcaga agatgacttc tccgcagagg aattcttcac
    75181 taactttgct cgcatctggc gaatgaaggc tcgtcctgag ttcatgcaga tgttggctag
    75241 tgtcgatgtc catgcaccag gtcacctacg caccaacatc cagttgccaa acttcgatga
    75301 attccatgaa acatttggcg ttcaagaagg cgacggcatg tggcgtgcaa aagaagaccg
    75361 tgtgattatt tggtaaaaat gcgaaaacag ctagtgaaaa ctggctgttt ttgtctttcc
    75421 caattccccc aaaacgtgat ataatagtaa agataattta ttgacatgga gaaatgatat
    75481 gagcttctac aatcacaagg aaatcgagcc caaatggcag ggattttggg caaaaaatca
    75541 tacttttaag acgggaacag atgcggctaa gccaaacttt tatgccctag atatgttccc
    75601 ttatccgtct ggtgcgggct tgcacgttgg tcaccctgag ggctatacgg cgacggacat
    75661 tctcagccga tacaagcgtg ctcagggcta caacgttctt cacccaatgg ggtgggatgc
    75721 ctttggtctg ccagctgagc aatacgctat ggatacaggc aatgacccag ctgactttac
    75781 ggcggaaaat attgctaact tcaagcgtca gattaacgcc cttggctttt cttatgactg
    75841 ggaccgcgag gtcaatacga ctgaccctaa ctactacaag tggacccagt ggattttcac
    75901 caagttgtat gaaaaaggcc tagcctatga ggcagaagtg cctgttaact gggtagagga
    75961 attgggaaca gctatcgcca acgaagaggt ccttcctgac ggaacgtctg aacgtggtgg
    76021 ctacccagtt gtccgcaagc caatgcgcca atggatgttg aaaatcactg cctatgcaga
    76081 gcgtttgctc aacgacctag aagaagttga ttggccagag tctatcaagg atatgcaacg
    76141 caattggatc ggcaagtcaa cgggtgccaa tgtgactttc aagattaagg acacagacaa
    76201 ggacttcaca gtctttacaa ctcgtcctga cacccttttt ggtgcgactt atgctgttct
    76261 tgctcctgag catgacttgg tagatagcat tacatcgcct gagcagacag aagctgtggc
    76321 agagtacaaa cgccaagctt ctctcaaatc agacctagcc cgtacagatt tggcaaaaga
    76381 caagacaggt gtttggactg gtgcctacgc catcaaccct gtcaacggta aagaaattcc
    76441 aatctggatt gcggactatg tactggcaag ctacggaaca ggtgctatca tggccgttcc
    76501 tgcccacgat gagcgtgact gggaatttgc caaacagttt ggtttggaca tcattccagt
    76561 tctggaaggt ggcaatgtag aagaagctcc ttacacagag gacggtgccc acatcaattc
    76621 tgatttccta gatggtctta acaaggaaga agctattgcc aaaatggtag cttggttgga
    76681 agaaaatggt gttggtcaag agaaaatttc ttaccgcctc cgcgactggc tcttcagccg
    76741 ccagcgttac tggggtgagc caattccaat catccactgg gaagacggca cttccacagc
    76801 cgtaccagaa aatgagttac ctctggtatt gccaaaaacc tcagacatca agccttcagg
    76861 tacaggggag tcacctcttg ctaacttgac agactggttg gaagtggtgc gggaagacgg
    76921 cgttaaaggt cgccgtgaaa ccaacactat gccacaatgg gcgggttcta gctggtacta
    76981 cctccgctat atcgacccgc acaatgatga gaagttggca gacgaggatc ttctcaaagc
    77041 ttggttgcca gtggacatct acatcggtgg tgccgagcac gcagtccttc acttgcttta
    77101 tgctcgcttc tggcacaagt tcctctatga ccttggcgtt gtgccgacta aagagccttt
    77161 ccaaaaacta tttaaccagg gtatgatttt gggaactagc taccgtgaca gccgtggtgc
    77221 ccttgttgcg acagataaag ttgaaaaacg tgatggttct ttcttcaaca tcgaaactgg
    77281 tgaggagttg gagcaggcac ctgctaagat gtccaagtcc cttaaaaacg tggtcaatcc
    77341 agacgatgtg gtcgagcagt tcggtgcgga taccctccgt gtttacgaga tgttcatggg
    77401 cccacttgat gcgtcaatcg cctggtcaga agaaggtctc gagggcagcc gtaagttcct
    77461 tgaccgtgtt tatcgcctct tgacgaccaa ggagcttgcg gcggaaaata gcggagcgct
    77521 tgataaagtt tacaatgaaa ctgtgaagac ggtgacagag catattgaag acctgaaatt
    77581 caacacggct atcgcccagc tcatgatttt tgtcaacgca gccaacaaag aagacaagct
    77641 ctatgtcgaa tacgccaaag gctttgtcca attgattgct ccatttgcgc cacacttggc
    77701 agaagaactc tggcaaggtc tgacaaatac aggtcagtca atcagctatg tcgcatggcc
    77761 aacatatgac gaaagcaagc tggtagaaag cgaagtggaa atcgttgtcc aaatcaaggg
    77821 caaggtaaaa gcccgcttga ccgtagccaa agacctagca ccagcagagc ttgaaaaagt
    77881 tgcccttgcg gacgaaaaag tccaagctga aattgctggg cagacagtgg tgaaggtgat
    77941 tacggttcca aacaaacttg tcaatatcgt taccaagtaa cgattgcctc gtttgtttgt
    78001 ctatttccaa cctccaacag tctatccgca gactgttgga gccaactaaa cttgttaata
    78061 ttgttgtgaa ataaatgtaa ataataagaa gacgacttcc gtattggagg tcgtttttgt
    78121 attttctaga atgatcatgt tgtcttaatt tccgttgtga tacggaaaaa gagggtctgt
    78181 gtatccgttt acaatagatg ttcactgaaa tataataaga atataaaaga agatgaggga
    78241 gggaaagcgt gttgaaaact cttaaattag tgacaattgg tggaggctca agttacactc
    78301 cagaattggt tgaaggtatg attcttcgtg cacatcaatt acccattcga gaatggtggt
    78361 ttgttgatgt tgaacaaggc gcaaaaaaac aggagatcgt agttgagtta gcacggcgaa
    78421 tggtcgcaaa agcaggcctg gactggaaga tagagtcgac cttagacaga agagaagcat
    78481 tgatagaggc tgattttgta acgtctcagt tccgagttgg ccaactacct gcaagatact
    78541 tagatgagac cattcccttg agttatggga tgctggggca agagaccaat ggggctggag
    78601 gaatctttaa agcatttagg accattgaag catataaagg gattatcaag gatatgaagg
    78661 acttgtgtcc gaatgcttgg ttgattaatt ttacgaatcc tgctggtatc gtaacggaag
    78721 ccattgctaa tgaactccac tggaagaaag ttattggttt gtgtaatatc cctattggtc
    78781 aaaagaaact tgctgctcag attttaggcc aatcagaagg agacctaagt acgcgccata
    78841 ttgggttaaa tcattttcac tttcatgaag tttgggataa gtatggggaa aatcgtacgc
    78901 aagtaatctt agaaacattg tatggggatt tgagagagga gacgccagaa gcagtcaaaa
    78961 atattaccaa tctagagttt cctattcaat tgcttcggac gattggaatg ttgccatgtg
    79021 attaccatcg ttattatttt ttggaacaag aaatgttaga ggatgctttg gagcagtatc
    79081 gtcagggaaa tgtacgagcc aaggttgttc aagaagtaga gacagaattg tttgagcttt
    79141 acaaagaccc acaattgaaa gataaaccaa aacaactgga actacgtggg ggtgcctact
    79201 atagtgatgt tgcctgtcaa atcatttgtg ctatccataa tgatagtaga gaagaattga
    79261 ctgtatctac tgtaaataga ggagttattc cctatctacc tgaaaattgt gtggtagaag
    79321 tcacctcgat tatctctgcg caaggagcaa caccactagc ttgtccacca actccgccga
    79381 ttgtaaccgg ctatttacaa ctgatgaagc aaatggagct tgtcactgtt caggcagcaa
    79441 tgacaggtga ttattcactg gctttacaag cttttacact caacccgctt atttcaaacg
    79501 gaccacaagc tgaagtgcta ttacaagata tgttacttgc acatgagaat tatttgccac
    79561 aattcgcacc agctatacag cacataaaag agagaaggaa caatcaatga ctaaagacaa
    79621 ggtattttat caatttccaa caaatttttt gtggggttct tctacatcag gacctcaaag
    79681 tgagggaatt gagccaggtg atggaaaagg acagaacaac tgggactatt ggtatagtat
    79741 tgagcctgaa cgatttcata acaaaattgg ccctagtcaa acatcaacat tttacaaaca
    79801 ttatgaaaaa gatattgatt tattagttga aactgggcac actgtatttc ggacctccat
    79861 tcagtggtca cgtcttatac caacaggtac tggagaaatt aatcaagagg cagtgtcctt
    79921 ctatcgttcg gttttttctt tggtaagagc aaagggaatt caactctttg tcaatcttta
    79981 tcactttgat cttccctatt gtttacaaga aaaaggtggt tgggaaaata aggaaattgt
    80041 tcgagcctat gaagcatatg cacgtacatg ttttgaattg tttgatgact tagtcgatcg
    80101 ttggattacc tttaatgagc cgattgttcc agtagagtgt ggttatctag gaaattatca
    80161 ttatccatgt aaggttgatg caaaagcggc agttgcggtg gcttatcata cacagttggc
    80221 aagttcttta gctgtaaagg catgccatga ggtaaatcct cacaaaaaaa tcgctattgt
    80281 cttgaatcta actcctgcct accctcgtag tgagcaagca gaggatataa aagcagctcg
    80341 aattgcagaa ttgtttcaaa caaagagttt tctagatccg tctgtgttag gagaatatcc
    80401 cgatgaatta gtcgagctga ttgatgcata tgggctaatg ccagaggtca gcgatgagga
    80461 attagccatt attaaggaaa atcgtgtgga cttcctaggg gtgaattatt atcaacccct
    80521 tcgggtacaa gctccaacca atagttggaa agagggagag cctcttactc cggaacattt
    80581 ctttgcccca tatgatatgc caggtaaaaa aatgaatccg catagaggtt gggagattta
    80641 tgaaaaaggg atatatgata ttgcgataaa tattaaggaa aattaccata atatcgaatg
    80701 gtttgtaaca gaaaatggta tgggagttga aggagagggc gcatttaaag acaatggact
    80761 cattcaagat gattaccgga ttgaatttat cgaagatcat ttaattgaat tacatcgagc
    80821 tatccaagaa ggggcgaatt gtaaaggata catgttatgg acctttattg attgctggtc
    80881 ttggttaaat gcatacaaga atcgctatgg attagttgaa ctggatctag acacgcaaga
    80941 aagaattgtt aagaaatcag gcaagtggtt caagaaattg agcgatgcta atggttttca
    81001 tcgctaggag ggaatgatgg tagaactggg cttttcaatt tatcctgaaa actattcgct
    81061 agaagagagt caagcttaca ttaaacttct gcaccaatac ggagcaaggc gcttatttat
    81121 gagtttgttg caacttgaaa atggagaccg aacagttttt gaattgtatc aaaaactagt
    81181 tgactatgca aatcaactag ggatgactgt cattgcagat atcagtcccc attttatcgc
    81241 agcgcaaggt tgggatgggg cattgatgga gcgtgcagct gaactggggt tggctgggat
    81301 tcgtcttgat gaagctcttc ctttggaaga aatcattgag atgacacata atccacacaa
    81361 cataaaagtt gaattgaatt tgagtacaga taggaccttg ctgtccaatc ttttggctag
    81421 tgttgcaaac agggataata ttattgcctg ccataatttt tatcctcatg cttatacagg
    81481 tttatcccaa gagcacttct tagagatgtc atctttttat tatcaatccg gtataagaac
    81541 tgcggcattt gtcagtgcac aaacagcttc tgaggggccg tggccattat ctgaaggtct
    81601 accaacttta gaagatcatc gattctggct tcttcccttg caaattgctt ggatgaaggc
    81661 aacgggtttt attgattcta ttttgatttc aaaccaattt atctcggagg aggaactaca
    81721 atccattcaa acagttttaa atgaagatac aatcagcctt atggttgata ttgaggaggg
    81781 tttgactccc gttgaaagag agattgtagc gtttgatcat gtttatagag gtgatatttc
    81841 tgcttatgtt ctgcgttcaa ccatgccccg tgtggtgtat aaggacgcat ctgtaccggc
    81901 tcggtcagac caagcaattc ctgtgggacg aggggatatc ttaattgata atgatttata
    81961 tggtcgctac aagggtgaat tgcaaattgc actcaaagaa tttaccatca gtccaaaggt
    82021 aaataaggtc ggacgaatca gcccagacta tcttcccttg ttacgcttta ttaaaccgtg
    82081 gcaagagttt aaactggtca tagcttccga gcactttcat taatgatgcg gaaaaagtag
    82141 gcacctctag aaatcgcttt catgttataa tgtacatata agaaatcaaa gactgttggc
    82201 ggaaaggagg gagcatgtta tctaaaaaag aagtacgctt gttagaggct ttacttgcaa
    82261 aacaggagac attcgtacca agcatcgagc tagccaaggt attatcagtg agtgatcgaa
    82321 cggtaagaac gtatattcat caactgacat cttctttaga aaatagcggc gctcatatcc
    82381 tttcaaaaca aggtagtggt tattgtttac agattgaacg gccagttgag tttgaaatgt
    82441 tttggcagga gcaattgctc ctaaaaaagc ggttaactga tttaactcaa ctagaagaag
    82501 cgggtgatcg gcaacggtat attctgaaca aactcttgtt cgaggatcca gttcagtctc
    82561 ctgaatgttt acaaagagag ttattcattg gaaagactac gcttcattcc attttggcgg
    82621 atattcgaaa actatttatc ccctatcaat tagaactcct tgtgacacgt aaaggaattg
    82681 aactaaaggg ggatgaacct gctatccgtc actttattat ggattacttt tttgatgagg
    82741 gaaatgttca atctgtttat ggggttgtcg aaaccaatct attgccagat atacggttca
    82801 cagacttaat gttaatcgtc ttagacgagt gtcgggatgc taaattacgt ttatcagatt
    82861 ttgtcatgca gaatctagtg ctccatatcg ctcttctttt acaacggttg aaaattggat
    82921 ctggtctaga ccggttccca ataccggagc gtattcagtc gtctcaagag tatcttgtgg
    82981 cacaacggat tattgaacga atcgaagaaa tgtttcaagt gacgataccg tcagaggaag
    83041 caaattacat tgcgttgcac cttaaggtaa aactaacggg gaatccaaat caagatggtg
    83101 aggtggaaga gatatttaga cagaatgtgg agaatagtct tttacatttg tcacatttaa
    83161 caggcctaga gttgtcaaag gatcagcagc tattcaaagg agttctggcc catttgatgc
    83221 cgttacgtat tcgtttggag aataatattc aactgggaaa tcccctaact gctgaaatta
    83281 aagaccaata ttttgaggct ttcgaattaa caaaaacggc atttgcagcg ataccagaac
    83341 taatggggta ttcgatttcg gatgatgagt gggcttacct taccttgcat gtgatggcgg
    83401 caattgaacg ttatcaggga tttaagaaga ccagagtatt ggtagtctgt gctaccggaa
    83461 ttggaagtgc attgatgtta aagaatcgct tggagaaaga attttcatcc agcttagaaa
    83521 ttcttgatac ggttagttat tatgaagtta cagaggaacg actaaaagat gttgatttga
    83581 ttatttcatc cattagcttg tcgaatctaa tttttatgac accggttatc aatgtcagcg
    83641 tcttcctatc agagaaggac attgaggcta ttcgatctca tttacagcaa acgaccccag
    83701 ttattcctga ccaaggggaa agtgtgctaa tggaagtagg tcaggctaag caaattgcag
    83761 aagcagtatt tgctccacat agaataatct atcttgagga ggattgtgat cgagaagtag
    83821 ccttgaagca actagtaact actttgaacg aatccattga gcctgatttt gttaatcgct
    83881 ttatagaaca agtgaattta cgagaatctt acagtccggt tgtctttggg gattggttag
    83941 ccttccctca tccagccttg ccgatgagtt attcagaaca aattgttgta gggatatata
    84001 agaatgctgt ggattggggc catggaaagc aagttcaatt cgtcttccta ttgtcaccgt
    84061 caaagggtcg aaatgcattt ctgaagtata tctcaccttg tctggttgat tttgttcaag
    84121 atagagttct ccaagaacgt ttgttacatc atccaaatta taaagaatta attgatattt
    84181 ttattcccct cattcaaaga aaggggtagg aggagttttt tatgaaacac attatgttag
    84241 tttgtaacgc aggcatgtct acaagtatgc ttgtaactaa gatgcaaaaa gcatcagaag
    84301 aaacaggggt tgaagcaact atttgggctg ttccagtatc tgaagccgat aatgaagttg
    84361 ctcaaaaaga gattgatgta ttactcttag gtccacaagt aaaattccta ttgaacgact
    84421 ataaggcaaa atttgagcca actattaagg tagatgctat caatatgatg gattacggag
    84481 tgatgaacgg agctaaggtg ctagaggctg ccctaaaaat gatggagggg tagtatgaat
    84541 tctccagagt atcttgaagc gattatgggg ttaatcatgt acggtggtga agcaaagggc
    84601 ttcgctagcc aggcgattca agcagccaaa gaaggtcgta ttgacgaagc ggaacagctg
    84661 ctccaagacg caatagcttc attgaatatt gcccataaat cgcaaacaca attattagca
    84721 aaagaagcaa gtggagagtc tgttgaatta tctttgttaa tggtacatgg acaagatcac
    84781 ttgatgaatg ctttgacatt tattgattta gcgaaagagt tcgtagatct ttataaacag
    84841 attggataat tataggagaa ctgggttatg aagatatcaa aaataggtca agttccaatt
    84901 gagcgacttt tcgctactat tcgccaatcc ttaaaagatg attttcagca aaatacgggt
    84961 tccatcttgc aagatcaaga cattatccag ggacttactt atactaaaac atttggtgat
    85021 aagggacaac atagtgtcaa ggtaactgta gaagagttta cagctcctta ttgctatgca
    85081 gtttgtttcc attctaatcg aggaaaagag catgttcgct atcagttaga ggatttaggg
    85141 aatgatcaaa ccaatataga atattcttat gagatggagg ctggtgatat ttttgtaaaa
    85201 ggcaatcaat ttctcatgga aaaaatcttt tcaaaaagta taaaacgtca gacaaatgcc
    85261 cagttagagg cgctgattcg ttgctcagtt gagccggtat cagtttaaca tttaggacat
    85321 ataaaacaaa aaatagaaaa ggagacagaa tatgtcaaat atttctgaga aattcatgga
    85381 aatatctggg aaaattggtt cgcaacggca tttgattgcg attcgtgatt cctttatttc
    85441 tatgatgccc attacgatgg caggtacagt agccgttcta ttgaacgtct ttctgagaga
    85501 catcccaaat aatatgggat ggactggttt tgctgatgcc atgcaaccag tgatagatat
    85561 aaatggctac gtttattttg gaaccatcgg gattatggca cttgtatttg cctttacctt
    85621 tggttacaat ttatcactca tgtataaggt gaatccttta gcaggtggtt tgatttcctt
    85681 cgcatcattc atctctacta ttcctcaaag tgtaacgatt tccactactt tagacggagt
    85741 tgataagtca gttgtttctg ctttggaagg tttgggatta tccattgtga cgacagatgg
    85801 agtttcagcc ttggaaacaa gccagtgggg agctatcgca ttgaaatatg gcggtgcaac
    85861 aggtttgttt acagcattgt ttattggatt tctcgctaca tgggtctatg caagtcttgt
    85921 aaaacggaat gtcattatta aattgccaga ttctgttcca ccggcagtta ataaggcctt
    85981 tgctgctatt atcccaggta ctgctgcaat ttatgcgtca tccattgtgg catatcttgc
    86041 atttactcta acaggacaat ctttgggaga tttagtatca acttatattc aactgccatt
    86101 acttggtctg tcacaaggtc ttgggtcagt cattctttta actttccttg tacaactttt
    86161 ttggttcttc ggcctgcatg gtcacaatgt cttggcgcca attatggacg gagtctatat
    86221 ggttgcacta aatgaaaatg cggctgctta tactgcaact catagtactg cgaatttacc
    86281 gtggctttgg actcgtggtt cctttgatgc ttatgctcaa atgggtggtt caggcgtcac
    86341 tctagctttg attattgcta ttttcatttt ctcaaaacga gaagaataca aaatggtagc
    86401 taaattatct gctccaatgg gtgtattcaa tattaacgaa ccagttacct ttggtatacc
    86461 aatggtattg aatccactgt ttgctattcc atggctaatt gttccaccga tttgtgcaac
    86521 tatcgcatac ttagcgacag cggcaggtat aattccgcca gtctttgctc ctgttccatg
    86581 gattacccct ccaggactct acgcttatct agcaacaggt ggtaacatta tggctgcgtt
    86641 ggtttcacta ttcaacctct ttgttgcctt cctgatttgg acaccatttg ttattgcagc
    86701 caataaggtt aaggctggtg aggaataatg ttttccaaga acaaatctcc tttcttattg
    86761 gcgctaggat ttttccttat cgcgattagt ccgctttatg ggaataaata tctaaacatt
    86821 atatctggtt tggtttttat tctaatcagt gtactcagga gtaggagaaa ataattacac
    86881 taacactaaa tagaaaacga cttccgtatt ggaggtcgtt tttttgacaa aaagtccgaa
    86941 cactgtgatt gttaagagat tgcaagttat ctgtaatatt tgttacattt ctttaagctc
    87001 tctgcaagga ttatccctta ggatagatag ggcagaaaaa acacgaagga atcaaatatt
    87061 atgagttatc aaatgataaa aaaacaattc aaattgggac tgtttacagc cctcatagga
    87121 aatcttttat ggttgggaat ggtgaatgtc agtgcagatg acacagttat aagcgatagt
    87181 ccgacagtat cagcgttaac aaaaaaggtt gaagctgcaa aaatagcagc caaagctgct
    87241 gcagaaaaaa ttgtggctga gaaagctgct gccgctgcaa aggctgcttt ggctaccaat
    87301 atggtgtcag aggttgctgt ggaatatacg gctaatacct atccagctgg tcagtgtact
    87361 tggggtgcca aagaaatggc tccttgggtt ggaaactatt ggggtaatgg tggagattgg
    87421 gcagctagtg cggcagcgct tggatatgaa gtgggaacta ctccaaaagt tggcgctatt
    87481 gctgtatgga cagatggagg ctacggtcat gttgcctatg taacagatgt ggcagctaac
    87541 ggtcacattc aagttaagga atcaaactat ggtggagtat actacccaag taatgtccga
    87601 ggtttctttg atccaacaac gactagcgaa ggaacagtca gctatattta cccaccagct
    87661 ggagtataat taatatgaga tataaagacc gagtaggtct ttttttatgc tccgatactt
    87721 gattaaaatt tttaatgtct ctgatagaaa aaatatattg gctggaaatt gttattcatg
    87781 atactattgt aaaatctgaa aaagaaagtg tgttttaatt atgaagaaaa tagctatgtt
    87841 tacaagttta ttgtttgcgg gtatcgtgtt agcagcctgc aattcatctg caggtatatc
    87901 gaatagtaca gaccaaaccc tcttggcaca aattaaggaa gaaggcgtta ttcaaatcgg
    87961 tactgaaggt gcctatgctc cttactctta ccacgatgag agcgggaaat tggtcggtta
    88021 tgatgtagaa gtagccgaag ccgttgctga gaaattgggt gttaaggtgg aatttgttga
    88081 aacagaatgg gattccatga ttgcaggttt ggatgcggcc cgttttgata ccatcgctaa
    88141 ccaagtcggt attacagatg agcgtaaggc aaaatacgat ttttcaacac catatacata
    88201 catctatgga gccttggtaa cacagaagga taatactgac attacaagct ttgcagattt
    88261 atctggtaaa aaatcagcca acagcttgac aagtaactgg gcagatttgg cgcgtgaaaa
    88321 tggtgcagaa gttgtcggtg tggatggatt ctctcaggca gttgaattgt taaatacagg
    88381 gcgtgtggat gctaccatca atgacaactt ggtgtacttg gattatgtga aacagcatgc
    88441 tgatgcaccg attaaggttg tgaccttgac ggatgacgta tcgacaacag ccttcccagt
    88501 tgcaaaagga aatgaagatc ttgcgaaaga gattgatgcc gccttggcag aattggctag
    88561 tgaaggaaaa ttagttgaaa tttcaaacaa atactttggt gaagacgtat caaaagctaa
    88621 gtaatcagga gaaacaatga cagaaagagt atggcaacta ttgcttgatt cattttcaca
    88681 gatacttgtt ccaggattgt tggtgactat cccactgaca atcctgtctt ttacctttgg
    88741 cttgctaatt gctattggga cggccttggt gcaaattgct cagattccca ttctcaaaca
    88801 agtggctcgt ttctatattt gggttgttcg agggacacca ctcttggttc aactctatgt
    88861 gatattcttt gggctaccaa gtctaggaat tgtcttggat gcctttccgt cggcggtttt
    88921 ggttttttct gtcaatacag gtgcctacgc tgcggaaacc attcgggcat ctatcgagtc
    88981 cgtgcctaag gggcagttgg aggccggcta ttctgtcggg atgagttttg cccagaccat
    89041 gcgtcggatt attctcccac aggcctttcg agtggccttc ccgcccttgt ccaacacctt
    89101 gattggattg gtcaaggata catcgctggc tgccaatatt accgttttgg agatgtttat
    89161 ggcaactcag cagattgcag caaggaccta cgaaccgttt gccctctatt gtgaggtggc
    89221 ccttgtttat ctactctttt caaccatttt gaccaagctt caagcctatg gtgagaagaa
    89281 gttagccgtt tactaggagg ttcagatgat tcaagcaagt ggagtaaagc tgaccttcgg
    89341 tggtcagcag gtgttaaaag gaattgatgt agaggtcaat ccaggtgagg tggtcgtcat
    89401 tttaggcccg tctggatcgg gaaaaacgac tcttcttcgt tctttaaact ttttggaaaa
    89461 agctgacgcg ggtcacttag tctttggtga ggacagctat gacttggctc agatgaccaa
    89521 gaaggaaatt gcaggcattc gcaagcgcac agccttcgtt tttcaaaact ataatctctt
    89581 tgccaacaag actgccttac aaaatgtgac ggaaggtctg attgtggctc ggaaagtacc
    89641 caaacaagag gctgaaagaa tcggtaaagc agccttggac aaggtgggac tatctggtaa
    89701 atatggtttc tatccaagcc agctatcagg aggtcagcag caacgggtcg gcattgccag
    89761 agccattgcg accaatccag aggtcatcta ttttgatgaa ccaacctcgg ccttggatcc
    89821 tgagttaatc ggtgaggtct tggctgtcat gatgcagttg gcaaaagaag gtatgaccat
    89881 ggtggttgtg actcatgaaa tgggctttgc gcgtgcggta gctgatcgag tcatatttat
    89941 ggatggtggt cggattatcg aagaaggtcg accagaagac atttttgacc gtccacaaga
    90001 agaacgtact aagcaattct tagctcgcat tcaaaaggta gatgttgagt aagctaagca
    90061 aattaattta tcttatttat ttccataatc gtacagaaaa tggacagaat ttgacaaaga
    90121 tacttcaatt gttagacatt gattggtcta gcggttggag tattttttgt ttagagggaa
    90181 tggatattct tatatagtac ttttagcacg aggcgatgag ccgcaggcat agatgaagtt
    90241 aggcaaggaa gctaaggaag taatagaagg aaaactaaat gacgataaat gtattcgaaa
    90301 ggtctttact ttttagtaat aaatagtata taataaccta aagaaaacgt ttgcacaatt
    90361 ttgactgttt gcttgctaaa agataagcaa cagaaataga attagggagg agttctatga
    90421 cgacattagc cgatgtggct aggttagcca atgtatcaaa gatgactgtt tcgcgtgtca
    90481 tcaaccatcc agaacaagtt acacaagagc tgcggacgct cgtaacagct gcgatggaag
    90541 aattaaacta taagccaaat gtggcggcca aggccttggc ccagcaacgg acactggttg
    90601 ttcaggctgt cattctcgaa aagatggatg tggtagagcc gtactatatc gaccttcttg
    90661 caggaatcgc tgatgagctc aatcatcgaa attatacctt gcagttggtg accaatccaa
    90721 gtcagattac tgatcaatgt gatggttaca ttgtgactgg tgctcgcaag aatgactacc
    90781 cttggttgaa agggctgcgg aagccattga ttttattcgg ggagaatcaa gaaggccttc
    90841 cttttgtaga ttcagacaat cgtttggcga ctaggtccgc aacccagttt gctttatcaa
    90901 aaggttatga gaggattgtt tttgtaggaa ttgacttgcc agaagccttt gaaaaaggga
    90961 gagaagaagg ctacaaagat gcgatgcagg gccgagaaac tcatctctat cgattggaaa
    91021 atcgttctcg tgttgcggaa gaatttgtga aaaatcttga ttatttacca gaaaacacct
    91081 gttttatctg cgcatcggat cgtctcgctc ttggggttac aagaggttta caagctctag
    91141 gaaagtctat tccgaatgac gttggggtga ttggttttga cggcttcttt ttagatcgga
    91201 tgtccagacc agccttgaca acgatgaagc agccgattcg tcaaatgggg gctcttagcg
    91261 tcaaacattt aatgacgata ttggatggca agcctctggc tagacagggg cattactgcc
    91321 aggcaacctt aattgaaagg gagactacac ctcagtagtc tcttttttgg tcagtgggcg
    91381 gtctaggagc aaaagaaggg ggagctattt attctatttt tgttagatag tggcccaaaa
    91441 cttgttaccg ctaacactta agaaaacgtt tgcaaagaat ccttggaatt gtatatactg
    91501 gtagtgtaca attcaaactt atttatatgg aggagtctat gaaaaagaaa tccgttcaga
    91561 atatgaaacg aacagggatg ctagagaaga aacagatttt tggcattcgt aaactaaatg
    91621 taggtgtagc ctctgttggg attgcggctg ctctgttttt aagtggagca ggtcaactag
    91681 tgcaggcaga ggaagttgtc ctccctgcgt caactagttc aacgactctt tcggaacagg
    91741 atggcggcgc ggtagaagcg accacaactg cgatagaagc tacagattct atggtagctg
    91801 aagcgacagc ggtaacagaa acagccgatg caactgctcc agaggaagct cctatcgaag
    91861 aaggcagcat tcgtctgcac tttgaaaatg tagatgaaac agcaccagaa agtcaaggac
    91921 tttggacttg gggaggagtg gcagagccgt ccgatagtaa tcaatggcca acggatacag
    91981 cgaatttttc aagcagtcag gtagatgact acggccatta cgttgatatt aaaaaatctg
    92041 agacaccagg aacgattggt tatctggttc tcaagaatgg ggaaaaaata acagaatcgg
    92101 accaaaaagt ggagcttctt gttccggaac aaaatgaggc ttggatctct agtgattata
    92161 gcgtgtctag ctatgagccg ctcaaagatg aaaatgtcct ccgtatcaac tacacccgtg
    92221 aagacaataa ttatgaaggt tggggcgttt ggacttgggg cgatacgact gaagcgagtg
    92281 atggctggcc aacaggtgcc gttgacttta aacttggtaa atatggcgct tatgttgaca
    92341 ttcccctctc gaatggttta gattccaagc ttggatttct gctaatcaat cagaataatc
    92401 cagatttggc aggcaataaa accattgatt tagcctttgc agaccgtaaa cggcatagcc
    92461 aaattttcct tcgaaacggt gacgacaaag tttacacaaa tccatacttt atcgaagaaa
    92521 aagtagaatt agatacaagc aaagctactc cgggtacaaa aaatgtgact gttgaggcaa
    92581 gtgtgaaagc accattcaac tacaatgaaa gcggcttggt atctgtaact gtcaccaacc
    92641 ctgaaaatgc tgaaatagtc aaaatggaag ttgatacaag tgccatcggc ggaggtcttg
    92701 tgccaatctc tactgagctc aatcgtgtga ccattaaagc gacgtctagc acagcgccag
    92761 gtacatatag tcttcctgta aaagtctatg ataaagacaa tggttactac gaaacaaaat
    92821 tggatgtgac cattacagag cgtgtcaagg cagatggtga aaaagactgg gacgagcaag
    92881 tcatctactt catgatgaca gaccgtttct ataatggaga caccagcaat gataagctgg
    92941 tagaaggaac ggtcaccaac ccacgcggtc tttaccaggg cggtgacttc aaaggagtga
    93001 cagccaagct ggactatctg aaagaattgg gtgtggattc tatctggttg actccgattg
    93061 tggaaaatat tccacagaat gttggtagcg taaaagatgg agagtactat gcttaccatg
    93121 gttattgggc atccaatttt gagaaactaa atccgcattt gggaagttta gccgatttcc
    93181 acgaattgat tgatgctgcg gcggaaaaag gtataaatat tgttgttgac gtggtgctga
    93241 accatgctgg ctatggtaca gaagaaacct ttgcaggtat ggtacggact aaggaagaag
    93301 acaagcaggg agatgaccaa ttaggctcat tatctggatt accggacttc aagacagaag
    93361 aagctgctgt tcgcaatcag ttggttgctt ggcaggcatc ttggctggaa cgctcaacaa
    93421 ctgccaaggg caactctatc tatgctttcc gtgtcgatac agtcaaacac gttgatgata
    93481 ccacatggca acatttcaaa aatgaattgg tagatagaga tcctgacttc cacttaattg
    93541 gagaaacatg gggagctaac tataaagata ccaagggtga cttgggaatc ggtactatgg
    93601 acagcttgtt ggactttggc ttcaaggata tcgctaagta cttggtcaat ggtcaactga
    93661 aagcagctgg aaaagagcta gaagagcgta gcaaggtttt gactagcgca gcttctctgg
    93721 gtcaattctt gggcagtcac gacgaagatg gtttcctcta cagtctcggt ggcgcagaaa
    93781 aagaaggaaa tctggacaaa ctgaaattgg ctgctagtct tttgattaca gctaaaggtc
    93841 agccagtcat ttactacggt gaagaagtgg ggcaatctgg gcaaaacaac tggccagttt
    93901 atgacaaccg gtacgatttc gattggagca aggtagagac tagcgacatc acagaccact
    93961 atcagaaatt attggctttc cgcaatgcca attcaacatt gctctcacgt ggtgatacaa
    94021 gcacccttgc tggaaatgat agccaaggtt ggcttatcag caaacgcagt taccaagatc
    94081 aagcagccta tcttgtcttc tcaaccaata ccgagagcaa ggaaatggcg cttgaagtat
    94141 ctagtaaaga tgtagttgtc acagatgcct acacaggaaa atcttatcaa gctatcgaga
    94201 aaggtggtaa atgggttgtt caagtcgaac ttccaacaat cggtcaaggt ggcaccatgc
    94261 tccttcagac agaagcaggt gacatcgtca atgcaagtgt gcaaggagca actgaagaac
    94321 caattgaagc aggttacttc cgtgttcact ttaaaactct accatctgat aatttatcta
    94381 gcctaggttt atggacatgg gatgatgtcg aaaaaccatc gtcagatgtt ggtgcttggc
    94441 caacaggtgc aaccaatttc agtactgcta agcaggatga ttatggctat tatctggaca
    94501 tcaagatgaa agatgaaaca gccagcaaga ttagtctgtt gattaataac acttctggtg
    94561 ataacatcac aggtgacaag accattgaac ggattagcac caagatgaat gaagcttggt
    94621 ttgatgaaaa ctaccaactc agcctctatc aacctctaaa agaaggctgc atccgtatca
    94681 attacttccg taccgatggc aattatgacc aaaaaggtct ctggatttgg ggtgatgtga
    94741 ctgaccttac cttgggtgac tggccaaatg gtattgattt tgaaaaccaa ggcaagtacg
    94801 gtgcctatat cgatgtcaaa ttgacagatt taccaagttc cattggcttc ttgctattgg
    94861 atgaaagcaa gtcaggagat gatgtgaaga ttcagcagaa agattatagc tttaaagatt
    94921 tgaaaaatca gacacaaatc ttcctcaagg atgatgatgc gactatttac accaaccctt
    94981 attttgtcaa taatgtccgt gtgacaggtg tttctcacgt tagcctaaca gccttagaag
    95041 cagcctttac aacattggaa ggtgcggata aggatagcat cttggagaaa ttgtctgtaa
    95101 cagataagaa tggtcagaca gttgctgtga cggatcttgt cttggacctt accagcaaca
    95161 aggtgcgagt cctcggggat ttcaaccaag aaaatgcggg ctataccctc aaatatggca
    95221 atgatagctt cacaacaact atgagctggc agttgaagga tgagctctat gcttatgatg
    95281 gagagcttgg tgcgcgtgtg cgtcaggctg gtagtgtcgt tgatatgacc ctatggtctc
    95341 caagtgcaga cagtgtggca gtcgttcttt atgataagga tgaccaatct aaggtggtcg
    95401 gtaaattggc catgaccaag ggggataagg gacaatggga cctcgaattg aacagtcaat
    95461 caggtcttgg tatcgcggac tatcgtggtt actactacca ttatgaaatc actcgcgggg
    95521 gtcaatctgt tcttgtttta gacccatatg ctaaatcttt agcggagtgg aatagtgatt
    95581 tggcagatac agatccatct tatcggattg caaaagctgc gattgtggac ccagcagaag
    95641 ttggtccaag tgatttgaac tatgccacaa ttcctaactt caatcagcgt gaagatgcta
    95701 ttatctatga ggctcacgtc cgtgacttca catccgatcc tgctatttct gatgaattga
    95761 cagctcagtt cggtaccttt gcggcctttg cagagcgtct cagctacctc aaagacttgg
    95821 gtgtgaccca tattcagttg ctaccagtta tgagctacta ttttgtcaat gaattgaaaa
    95881 atgcagagcg gatgagtgag tatgcttcta gcaacagcaa ctacaactgg ggctatgatc
    95941 cacagagcta ctttgccttt acaggtatgt attctacaga tccgaccgat ccaatgaaac
    96001 gcatcgagga attcaaaaac ctggtcaatg aaatccacaa acaaggcatg ggtgtgattt
    96061 tggatgtggt ctataaccac acttctaaga ccttcttatt tgaagactta gagccaaact
    96121 attatcactt catggaggca gatggtacgg ctaaatcaag ctttggtggt ggtcgtctcg
    96181 gaacaactca ttatatgagt cgccgtgtcc tcgttgattc tatcaagtat cttgtggacg
    96241 agtttaaagt agatggtttc cgctttgata tgatgggtga ccatgatgcc gaagcaattg
    96301 agctggcctt taccgaggca caaaaactca atccaaacat cattatgctt ggtgaaggat
    96361 ggagaacctt tacaggtgat gccaatcagc ctgttcagcc agcggaccaa gactggatga
    96421 gttcaacaga tacggttgcg gtattctcag atgaaattcg caatacactt aaatctggtt
    96481 atccaaacga aggtcagcca gcctttatca caggcggtgc taagagtgta gaatctgtct
    96541 tcaataacat caaggcacaa ccaggcaact tcttggcaga tgatccaggt gatgtgatcc
    96601 agtatattgc tgcgcatgat aacttgaccc tctttgatat tatcgctcag tccatcaaga
    96661 aggatccatc aattgctgaa aattacactg aaattcacca acgtcaacgt ttgggtaatt
    96721 tgctagtctt gactgctcaa gggacaccgt ttattcattc tggtcaggag tatggacgga
    96781 ccaaacagtt ccgccatcct gattacaagg aacctgtttc agaggacaag gtaccgaaca
    96841 aagctcattt gttgacaaat gcagatggta cgccatttga ctatccatac tacattcatg
    96901 attcttacga ttcatcggat gcggtcaaca agtttgactg gaccaaagcg acagatgagg
    96961 cgctctatcc agagaatacc cgtactcagg cctttacaaa aggattgatt gccttgcgca
    97021 aatctacaga tgccttccgt ttgggaacaa aagaagaagt tgaacagaag gttagcttga
    97081 tttcaattcc aggtcaaaat ggcattgcca aaaatgatgt ggtcattgcc tatcaaacca
    97141 ttgcaagcaa tggggaccgc tatgctgtct ttgttaacgc cgacagtaag gagcgcagct
    97201 ttgttctttc tgatatttac aaagaattgc ttaagggtca cgtgctggtc gatggtaagc
    97261 gggctggaat agcagcgctg actgatttgg taggtgtgac attgacagat gatgcagttg
    97321 tgcttgcacc gttgactgca acggtgattc gcttgcctta catcactacg gcagttccag
    97381 atacagcgcc tactgctgaa gaaaaaccaa gtctagactt tactaccaag gaacgtacag
    97441 aggaaaaaat tcttcctatc gtagaggaaa ttcgctatga tgcaagtctc tctaccgatc
    97501 aatcttatgt ccttcaagaa ggcaaaactg gtaagaaagt tctggtttat caggatgtct
    97561 tgattgatgg taaggtagtt gctactaatt tgttatcaga aacagttgtt gatagcgaaa
    97621 cacgtattgt tgtgaaaggc agtatggagg ctaagaaaga ggctgaaaaa ccgagtcttt
    97681 ctactactgc tcaagcatct ggacaagcga caagtggtgc aagcaaggcg agtctgccga
    97741 ttacgggtga ccgacaaagc gatttggcac ttctaggtct cggacttgct ggacttggtt
    97801 tgacagttgc tgcgcaagga agaactaaaa aatcagaaga gtaataatat ttggctctcc
    97861 agaggagcat tgctcaaaat cttgtgaatg tcttataaat acaagagttg agagtatcac
    97921 ttccctctag aagaggttgg tttcaaacca acctcttctt ttatgttttt tgaggttctt
    97981 ttaaattttt aggggaaata acgtctattt attgattatt agaataaatg ggctggtgaa
    98041 ctatagggag gaattctgat taaatcagcc attaaagttc ggaaatagtc caatagtgaa
    98101 cacaagcctg tgctttattt ggtctttttg tttccgctta cataacttat aataaaacca
    98161 taaaaatcat ttcaaaaaag gagaatgaaa aatggatttc cttcaaaccc cattaaactg
    98221 gttctcacag aatatcctgc agaatccagc cttcttcgta ggtcttttag tattggtggg
    98281 ctatgccctc ttaaagaaaa agcctcatga tgtttttgca gggtttatca aagcgacagt
    98341 cggctatatg attttgaacg tagcggctgg tggtttggta acaaccttcc gtccgatttt
    98401 ggcggccttg aactataagt tccaaattga tgcggcagtt attgaccctt actttggtct
    98461 tgcagcagca aatgggaaaa ttgctgagga gttccctaat ttcgtatcag cagctactac
    98521 agcactcttg attggtttcg gtgtcaacat cctcttggtg gcactacgta aggttaccaa
    98581 agtacgtact ctgttcatca ctggacacat catggtacaa caagcggcaa cagtctcatt
    98641 gatggtgctc ttcctcatcc cggctcttcg taaccaattc ggtacagcag cgatcggtat
    98701 catctgtggt atctactggg cagtcagctc aaatatgaca gttgaagcaa cgcaaagatt
    98761 gacaggtggc ggtggatttg ccatcggtca ccaacaacaa ttcgcaatct ggtttgtcga
    98821 taagattgca ccaaaacttg gtaaaaaaga agaaaacttg gataacttga agctacctag
    98881 cttcctcaac atcttccacg atacagtcgt tgcttcagcg acattgatgc ttgtgttctt
    98941 cggtgtgatt ttgttcatct tgggtccaga aatcatggca aatccagaag ttatcacttc
    99001 aggtgctcca tacaacccag ctaaacaagc cttctttatg tatgtgattc aaacagcctt
    99061 caccttctca gtatatcttt tcattttgat gcaaggtgtc cgcatgttcg tagcagaatt
    99121 gacaaatgcc ttccaaggta tctcaagcaa attgcttcct ggttcattcc cagcggtgga
    99181 cgttgcagct tcttacggct tcggttcatc taacgcagtt ctttcaggtt ttgccttcgg
    99241 tttaatcggt cagttgatta caatcgtcct cttgattgtc ttcaagagtc caatcctcat
    99301 catcactggt ttcgtacctg tattcttcga taacgcagct atcgcagtgt acgctgacaa
    99361 acgtggtggt tggaaagcgg cagccatcct ttccttcatc tcaggtgtcc ttcaagttgc
    99421 tcttggtgca gtctgcgttg gcttgcttga attgagcggt ggttaccacg gaaacattga
    99481 ctttgaagtg ccatggttac catttggatt tgcatttaag tacctaggta ttgctggtta
    99541 tgttcttgtc tgcctcttct tattggctat tccgcaactt caatttgcga aagcaaaaga
    99601 taaggaagca tactatcgtg gtgaggcaca agcagaatag tatccttggt ctgtaaaatc
    99661 agagcataat gtattaaaaa taaaatagga gtgaaattat ggttaaagtt ttaacagcct
    99721 gcggaaatgg catgggctca tctatggtca tcaagatgaa ggtagaaaac gcccttcgtc
    99781 aactcggcgt aacagatttt caatcagctt cttgctcagt cggtgaagca aaaggtttag
    99841 cggcaggata tgatattgtc gtagcctcta accacttgat ttctgagttg gaaggtcgca
    99901 cgaaaggtca tttggttggt cttgacaacc tcatggacga caacgaaatc aaaacaaaat
    99961 tacaagcagt gctataaata gccgttaggg gtagggagtt tccctccccc tttcatatag
   100021 cgtgtagaag ggaaaaacta tgaatttaca aaaatcattt acagaaaata attccattcg
   100081 cttgggattg acagcggaaa cctggcagga ggctgttcac aaggctgttg agcccttgat
   100141 tgacagcggt gctgcgacag aagaatacta cgacgcgatc atcgcatcaa ctgaagaata
   100201 tggaccttac tatgtgctca tgcctggtat ggctatgcct cacgcccaag caggtgtcgg
   100261 cgtcttgaag gattcctttg ctttgattac cttgacaaaa ccagtcgtct tctcagatgg
   100321 gaaagaagtg tctgttctgc tcactcttgc agctaccgat ccaaagattc atacatccgt
   100381 agctattcca caaatcattg ccctctttga attggaaaat tcgattgagc gtttgattgc
   100441 ttgtcaaact cctgaggaag tcttggcaat ggtggaagag tccaaaacca gtccttacct
   100501 agaaggttta gatttagaaa gttaggagaa aaaacatgaa acatattcca aatttacaag
   100561 tcgcccttga ccattcagac ttgcagggag cgattaaagc agctgtctcc gttggtcatg
   100621 aagtggatgt tatcgaggca ggtaccgttt gtttgcttca agttggtagc gaattggtag
   100681 aagttcttcg tagcctcttc ccagacaaaa ttatcgttgc agataccaag tgtgcggatg
   100741 cgggtggtac tgtggctaaa aacaatgctg ttcgtggtgc ggactggatg acttgtattt
   100801 gttcagcaac tattccaact atggcagctg ctttgaaggc tatcaaggaa gtgcgtggcg
   100861 acaagggtga aatccaaatc gaactttacg gtgattggac gtttgaacaa gctcagcttt
   100921 ggttggacgc aggtatttcc caagcgattt accaccaatc gcgtgacgcc ctccttgctg
   100981 gcgaaacctg gggtgaaaaa gacctcaata aagtgaaaaa attggttgac atgggcttcc
   101041 gtgtttctgt aacaggtggt cttgatgtgg ataccctcaa actctttgag ggtgtagatg
   101101 tcttcacctt tatcgcaggt cgcggtatca cagaagcagc agatccagca gctgcggcgc
   101161 gtgaatttaa agacgaaatc cgtcgtatct gggggtaagg cctatggcac gaccaatcgg
   101221 aatatatgaa aaggcaacgc ccaagcattt cacatggaag gaacgcttgg agtttgccaa
   101281 agaattaggt ttcgactttg tggaaatgtc ggtggatgag agcgacgctc gtttggctcg
   101341 tttggagtgg accaaggaag agcgtttaga cttggtcaag gccatctatg aaacaggtgt
   101401 ccgcattccg accatttgtt tctcaggtca ccgtcgctac ccgcttggct ccaatgcccc
   101461 tgcacttgaa gccaagtcct tagaaaccat gaaacagtgt atcgaactgg ctcaggattt
   101521 gggcgttcga gtcatccagt tggctggcta tgatgtttac tacgaggaaa aatcacctga
   101581 aaccagagag cgtttcatca agaatctccg tcaggcttgc gactgggcag aacaggctca
   101641 ggtcatgttg gccattgaaa ttatggatga tccatttatc aactccattg aaaaatacct
   101701 agctgttgaa aaagaaatcg actcgcctta tctctttgtc tatccagata cgggaaacgt
   101761 gtctgcttgg cataatgaca tttatagcga attttatctg ggacaccgtt ctatcgcagc
   101821 ccttcacctc aaggatacct atgcggtaac tgaaaattcc aaaggtcaat tccgtgatgt
   101881 cccattcggt cagggctgtg tggactggga caatatgttt gcagtattga aaaagaccaa
   101941 ctaccaaggt ccattcttga ttgaaatgtg gtcagagaat tgtgaaacgg ttgaggaaac
   102001 acgagcagct atcaaggagg cacaagattt cctctatcca ttgattgaga aagcggggtt
   102061 gaactaatgc caaaagattt acaggaaatg cgtcaacggg tctatgaagc caatatcgcc
   102121 ctgcctgctc atgggctcgt caagtttacc tggggcaacg tatcagaagt ctgccgtgaa
   102181 cttggtcgca tcgtcatcaa gccgtctggt gtggactatg agaaattgtc accagaaaat
   102241 atggtcatta cggacttgga cggaaatgtg gtagaaggtg acctcaatcc ttcgtctgat
   102301 ttggcgaccc acgttgccct ctacaaggct tggccaaatg tccatgccgt tgtgcataca
   102361 cattcgacag aagcagtggg ttgggctcag gcaggtcgtg acattccatt ttatgggacg
   102421 acccatgcgg actatttcta tggtccggta ccatgtgctc gttctttgac ggctgaggaa
   102481 gtgaactcgg cctatgaaaa agaaacagga gcagtcatca tcgaggaatt tgaacgccga
   102541 ggtattgatc cagtggccgt tccgggtatt gttgtccgta accacggacc atttacctgg
   102601 ggcaaggatc ccgctcaggc agtttaccat agcgttgtcc ttgaggaagt ggctcggatg
   102661 aaccgctata ccgaacaaat caatccacgg gtggagccag cgccaagcta tatcatggac
   102721 aagcactacc tccgtaaaca tggtcccaat gcctactatg gacaaaaggg tgatgaacat
   102781 taagttcttg tgtgaaaaaa acagcaggga aaagtagcag actccccaaa aaggagaact
   102841 gctttttccc ttttgatgag gagaaagatg aggaaatttt tacaggatag tctgacagag
   102901 acagcctact atttagaatt gattttatct gccatacttg gcttagcact tttagccttg
   102961 tcaacgcttc tttttgcaga cctactcagc tcattagctg gtgatattca agcggatacg
   103021 tttattcaaa attttctgag tcgtgcaatg accttggcag tcggagtcga atttatcaaa
   103081 atgctttaca aacagtcacc tagcaccgtg attgaggtac tcctggttgc tattgctcgc
   103141 caactgattg tagagcatgg ttctagtagt gattacctga ttggaattat ttcggtcgcc
   103201 atcctatttg cagttagaaa atacctcttc actcagtttg atgattccaa taatattatc
   103261 atgcgcgcca gccagaaagt aaaagtggct aatatcatcg cacgagtaaa aattccaagt
   103321 gaaggtagcg agactttgag agattttgtg acacgaaaac tcaatgaaga agagaaaacc
   103381 attgctattg gtgcctgtgt atatttgaaa aatgttgccc ttcggataga taacatgcac
   103441 ggagaactga ttacccgggt agaaattatt aaaagcatct attaattcta actacaaata
   103501 tttatgcaac ataaagcaag taatgatcag atgaatttta ataattatta gaacatccca
   103561 gataattatg ttaagatagt aacgggacgg gggaaagagg aggagaatct tgttattaga
   103621 taagagtagt tgcgctctac tgaggtacct gattggtctg caggaacctg agacaatcat
   103681 ggctatttcc aagggcttgc aacaatcacg tcgaaagatc tattaccatc tagaaaaaat
   103741 aaacgatgcc ctaccaacaa ctgtagagag tattgaaagc ttacctcgcc tcggtattcg
   103801 acttaccgaa gcgcaaaaac tagcctgtcg agatcttctc gattccattg attcgtacac
   103861 ctacgtcatg agcatgcctg agcgcattca gctgatgttg gtatatatct gtatcacacc
   103921 agaaagagtg accattgaac gtctaatgga gttgacagaa gtctctcgta atacggtctt
   103981 aaatgatttg aatgaaattc gaacccagtt ggctcaagaa caatacaaga tgaccctcta
   104041 tgtgtctaag agtcatggct atagtctcgt ttgtcaccct ttaaataaaa tccaatatgt
   104101 ccactcactt ctctatacaa tcttcttgga tgctaataaa ggttttttgc aggttctaga
   104161 agataagtta tttgagttgg tggggtgtca gttgctcttc tcagaagaaa tggcacaatt
   104221 tttacaggga caagtatcgc agctagaaaa gaaactgggt aagaaaatca atcgctacga
   104281 aatcgagttt atgctcaaag tattgccttt cttgctcctt agctatcgca atatggagct
   104341 ggatgacttg gaagaacgag ccattctcaa agaatttgaa caagttcgag agcgtatcga
   104401 gtaccgagtt gctcaggaat tgaaagatgc cttgttgcaa acctttgatt tgaaattgga
   104461 tgaaatagaa atctccctga ttgccatttt acttctatcc tatcgaaagg acagcgatgt
   104521 tcatgtattc agccaagatt tcattgactt gaagcgagtg gtagagcgat ttattcatca
   104581 ttttgagata cattcaccat ttgaattgga aaatcctgac gaattagcca aacatctctt
   104641 ggcacattgt aaggctctgt tgttcagaaa aacctacggc atcctatcta aaaatccaat
   104701 ggtccaacaa atccaagata aatacagcac cctgtttgct atgacacggg tttctggaaa
   104761 gattttggag gaggagtggc agattgagtt atccgatgga gatattgcct atttgacgat
   104821 tcatctagga ggagctattc gacgcagtgg gacgcgcaag gcacagacac agattgtcta
   104881 tttaatctgt gatgaagggg tgtcagtcca acgcttactt tataaacaat gccagcatca
   104941 tttgccagat aagaagattg gcgccgtctt taccactgaa caattcaaga gtgtcgagga
   105001 cttactagag gttgatttct tgattacaac cagcgatggg ttggaaacgg atttcccttt
   105061 ggtgcaggtt caccccattt tagattttga tgatgtgcta aacatgactc attttgcaaa
   105121 atatcgtagt ttggcagatg aaaaacaagt ttttgcagta gaactagaca agcttttgtc
   105181 gggatatatc gcagacggaa ggaccgctcg agaactcaaa caacggctac aaaaactgat
   105241 taccaatgaa ttattaacca atttatccag tcaagatatt gaaacagatc tgtattaggg
   105301 taaagtccaa gtttgaacac aagcctgtgt tttatttggt cttttttttg ttgcctttgg
   105361 agcatatact agaagcatag aaacgtacct tataggagga atggaaaatg gctaaagttc
   105421 aagatattac aagagaatcc tggattcttt caaccttccc agaatgggga acttggctca
   105481 atgaagaaat tgaagaagaa gtagtgccag aaggcaactt tgctatgtgg tggcttggaa
   105541 actgtggtgt ctggattaaa acaccaggtg gagctaacgt ggtcatggac ctctggtcat
   105601 cacgtggaaa atccactaaa aaagtgaaag atatggtttg gggacaccag atggctaaca
   105661 tggccggtgt gcgcaaattg caaccaaact tgcgtgtgca gccaatggtt atcgatccat
   105721 ttgccatcaa tgaattggac tactatctag tatcgcacgt ccacagtgac cacatggaca
   105781 tcagcacagc cgctgccatc atcaacaatc caaagttgga ccacgtcaaa tttgtcggac
   105841 cggttgaaag tggtgacatt tgggaaaaat ggggtgttcc agctgaccgt atcatccgta
   105901 tcgcaccagg tgatagcttt gagttcaaag acatcaaggt ccacgctgta gaatccttcg
   105961 accgtacttg cttggtgacc ttaccaatcg aaggttatga cgaaaatggt ggtaaattag
   106021 caggacttcc tgtgacagat gaactcatgg ctcgcaaggc agtcaactat gtctttgaaa
   106081 cgcctggcgg aaccatctac cacggtgcag actcccactt ctcaaactac tttgcaaaac
   106141 acggtcgtga cttcaacatc gacgttgcta tcaacaacta cggtgacaac ccaatcggta
   106201 tccaggacaa gatgacatca atcgaccttc ttcgtatggc agaaaacttg cgtgctaagg
   106261 taatcatccc tgttcactac gatatctggt ctaacttcat ggcgtcaaca gatgaaatct
   106321 tgcagctctg gaaaatgcgt aaggaacgct tgcaatacga tttccatcca ttcatctggg
   106381 aagttggtgg caagtatact tatccgatgg acaaggaccg tatcgaatat caccacccac
   106441 gtggctttga tgattgcttc ttagaagact caaatattca atttaaggct ttgttataag
   106501 atttagaact ctcttgcccc atagagacga ggagaggcac tataaactct gataaagtat
   106561 gaaatgtgcg aattactact gttcactcca agggcgggac gaaagtcctg cctcttttct
   106621 acggacaatt tatttctcgc ttcatttcaa acagacatag aaaacgcttt atggtataat
   106681 aaattcatga ctttaaaaaa ggagaaaaac atgtcaaatc tttcagtcaa tgcgcttcgt
   106741 ttccttggta ttgatgccat cgaaaaatca aaatcaggtc acccaggtgt cgtcatgggt
   106801 gcggctccaa tggcctacag cctttttact aaagaacttc gtatcaaccc agctcagcca
   106861 aactgggtga accgtgaccg ctttatctta tcagcaggtc atgggtcaat gttgctttac
   106921 ggacttcttc acctttcagg ttttgaagaa gtgagtatgg atgagatcaa gaacttccgt
   106981 cagtggggat caaaaacgcc aggtcaccca gaatttggtc acacagcagg tgtagatgca
   107041 acaacaggtc ctcttggaca aggtatctca actgcgacag gttttgcaca agcagagcgt
   107101 ttccttgcag ctaagtacaa ccgcgatggt ttcccaatct ttgaccacta tacttacgta
   107161 atctgtggtg atggcgactt gatggagggt gtttcagcag aagcggcttc ttacgcaggt
   107221 cttcaaaaat tagaaaaact catcgttctt tacgattcaa atgacatcaa cttggatggc
   107281 gaaacaaaag attccttcac agaagatgta cgtgcccgtt acaatgccta tggctggcat
   107341 acagacctcg taacagacgg tacagatgta gatgccatct ttgctgctat tgaaaaagct
   107401 aaggcagcag gtaagccatc tcttatcgaa atcaagactg ttatcggtca tggttcgcca
   107461 aacaaacaag gtacaaatgc cgttcacggt gctccacttg gtgcagaaga aacagaagca
   107521 actcgtaagg cgcttgattg gaactatgca ccatttgaaa ttccagctga agtctatgcg
   107581 gacttcaaag ctaatgtagc tgatcgcggt gcagcagcct acgatgcttg ggtgaaatta
   107641 gttgaagact acaaagtagc acatccagaa ttagcagctg aagtaacagc tatcttggaa
   107701 ggtcgtgacg tagttgaaat taagccagaa gacttcccgg tttacgaaaa tggcttctca
   107761 caagcaactc gtaactcatc acaagatgcc ctcaacgcag cggctaaagt attgccaact
   107821 ttcttaggtg gttcagctga cttggctcac tcaaacatga cctacatcaa ggaagatggt
   107881 ttgcaagatt cagtaaatcc actcaaccgc aatattcagt tcggtgtgcg tgaatttgcc
   107941 atgggaacta tcttgaatgg tatggcagcc cacggcggac ttcgtgtcta tggtggtacc
   108001 ttctttgtct tctcagacta tgtaaaagca gcagttcgtt tgtcagctct tcaaggactt
   108061 cctgtgactt atgtcttcac tcatgactct atcgcagttg gtgaagatgg tccaactcac
   108121 gagccgattg agcacttggc aggacttcgt gctatgccaa acctcaatgt cttccgccca
   108181 gcagatgcgc gtgaaacaca ggcagcttgg tacttgtcat tgactagcaa gtctacacca
   108241 tcagcccttg ttctcactcg tcaaaacttg acagttgaag aaggaacaga ctttgacaag
   108301 gttgcaaaag gggcttatgt tgtctatgaa gtagcagggt ttgatacaat ccttcttgca
   108361 tctggttcag aagtgaactt ggctgttaaa gctgccaaag aactagaagc agcaggtact
   108421 aaggttcgtg tggtttctgt gccatcaact gaactcttcg atgcacaaga cgcagcttac
   108481 aaggaagaaa tccttccaaa tgctatccgt cgtcgcttgg caattgaaat gggtgcaact
   108541 caaagttggt acaagtatgt cggtcttgac ggtaaagtcc ttggtatcga cacattcggt
   108601 gcctcagctc cagcccaaac agttattgac aactacggct tcacagtaga aaatgttgtg
   108661 aaattagttg gcgaattgta atagaaaatg tcagagacag cctttcgagg ctgtttttga
   108721 atttttttaa aaattttctt gacaatatcg acaatagatg atatattaat atctgtaaat
   108781 atcaatattg ctccccctac cttctcgact gaggaggtat ttttatattt ttaagttaat
   108841 agaagggcag aattttgaaa gaaatgtgtg ataaggaaga agtagtgtta gattgatttt
   108901 gatagcagag aataaagaag tgattttgaa ctgttatcag ggctgtgcta taatagattt
   108961 atgaactata cacatgaaga aaaagagtat tttatgactc aagccttgca ggaagctaga
   109021 aagtcgctag aaaaggatga aattccaatt ggttgtgtga ttgtcaagga tggccagatt
   109081 attggcagag ggcataatgc gcgtgaggaa ctcaatcagg caatcatgca cgctgaggtt
   109141 atggcaatcc aagaggctaa caatgttgag gggaattggc gcttgcttga ttcgaccctt
   109201 tttgtgacga ttgaaccttg tgtgatgtgt agcggagcta ttggcctagc acgcattccg
   109261 caggtcatct atggagcaac caacaaaaag tttggagctg cggggagcct ctatgacatt
   109321 ctgacagatg agcggctcaa ccatcgtgtc aaggtagaaa caggtatttt agaagcggaa
   109381 tgtgccaata tcatgcaaga ttttttcaga caacgcagag aaagacagaa ggctgctaaa
   109441 cttatcatga agacgcaaga ggaagcaact tcggatagct agttaatatt ccaaacttgc
   109501 actctggcca aattgtgtta taatattaaa cggagcaaca ttcgtgcgtg aagcgggtca
   109561 ggggaggaat ccagcagccc taagcgaggt cgaatgtgtg ctctttttgc atataatcta
   109621 aaaacccttt gaaatcagga tttcagaggg tttttctttg gtttattttt agaaatgagg
   109681 ggcaaaatag gggcaactta agaaaacttt ttggatgctc tgctattcta ctgaggaata
   109741 aggaggaaag cctatgtttt tatttgcctt tccaggtatg ggaaaaacga cacttgctaa
   109801 gaaatataca gatgttgtgg acttagaaat gtccgacatt aagtacgata atagcagtgt
   109861 tcgtcattta acgaaagagg agcgaaaatc caccaaacgc acactgaagg ataaaggcta
   109921 taaaacaatc tatgtcaata aggcctacac tcttcataga caaggaaaga cagtcctagt
   109981 agcgctgaat tttctgatga gaatgttgct ggccatgtct atacgcggtg gtgtgccatt
   110041 tcatatctat attccccacc cttctctcaa agaagaatac cgccaacgtt acatttcaag
   110101 aggcaacaac gaccgtttca tctttgaagt catgttcatt tggtatcctg ccctcattcc
   110161 gctttaccta ctatctaaga ttctcccgaa atggattaca gtaacccagt cgggagagac
   110221 tttggaggat tattacaaac gcgaaaattt cgttagattt tcacaaaaat taagatttat
   110281 acaacatatt tcatgaaaat aaatgaaaat tgataaattt cttgaaaaaa atatgataaa
   110341 gcgttatcat attatggtac ataaatggag gaaaaacatg acacatatta catttgatta
   110401 ttcaaaagtc ttgggccaat tcgttggagc tcaagaagta gaatacatgc aaccacaagt
   110461 tacccttgca gaccaaatgc tccgtcaagg aacaggtcct ggtagcgact ttatcggctg
   110521 gttggatttg ccagaaaact acgacaaaga agagtttgct cgtatcaagg aagctgcagc
   110581 taaaatccaa aatgaaagtg aagttttggt agttatcggt atcggtggtt cttaccttgg
   110641 tgctaaagca gctattgatt tcttgagcaa tgcctttgta aacttgcaaa cacgtgaaga
   110701 gcgcaaagct ccacaaatcc tttacgctgg aaactctatc tcatcaagct atcttgctga
   110761 cttggtggat tatgttgctg ataaggattt ctcagttaac gtgatttcaa aatcaggtac
   110821 aacaactgaa cctgctattg ccttccgcgt cttcaaagaa ttacttgtca agaaatacgg
   110881 tcaagaagaa gctaacaagc gtatctatgc gacaactgac aaggcaaaag gtgctgttaa
   110941 ggttgaagcg gacgcaaatg gttgggaaac ctttgttgtt ccagatgatg tcggtggccg
   111001 tttctcagtc ttgactgcag ttggtctttt gccaatcgcg gcagctggtg cagacattga
   111061 tgcccttatg gaaggtgcaa acgcagcccg cacgacattt acatctgata agattgctga
   111121 aaaccaagca taccaatatg cagctgtccg taacatcctt tacagaaaag gctatgtaac
   111181 agaaatcttg gctaactacg agccatcact tcaatacttc agcgaatggt ggaaacaatt
   111241 ggctggtgag tcagaaggta aggaccaaaa aggtatcaac ccaacatctg ctaacttctc
   111301 aacagacttg cactctcttg ggcaattcat ccaagaagga aaccgcaata tctttgagac
   111361 agtggttcgt gttgacaaac caagaaaaaa tatcctcatt cctgacatgg cagaggacct
   111421 tgacggtctt ggctacttgc aaggtaagga tgttgacttt gtaaacaaaa aagcaacgga
   111481 cggcgtgctt cttgctcaca cagatggtgg cgtaccaaac atgtttgtga cccttccgca
   111541 acaagatgag ttcacacttg gttacacctt ctacttcttc gagttggcga ttgctatctc
   111601 tggttacctc aatgcgatca acccatttga ccaaccaggt gtagaagcct acaagaaaaa
   111661 catgttcgct cttcttggaa aaccaggatt tgaagaactc ggtgcagaat tgaacgcacg
   111721 tttgtaatag aatgaaactg atcggaaacg gtcagttttt ctttgcttat taaatatggt
   111781 agaatagagt ctatgaaaaa agattttttt gtaatcggcg atgtccatgg aaagtttgaa
   111841 ctgctactcg atattttgaa gaaatggaat gaagaacgac aacagctgat ttttcttggg
   111901 gatttaattg accgtggaga aaattcaaaa gcttgtttgg agttggtctg gcaattagtc
   111961 cgagaaaaag atgctatctg tctgactggg aatcatgaac gaatgttttt ggcttggttg
   112021 cgggatccct tggaacgcta tgaccattac cggaggaatg gtggagatac gaccatcaac
   112081 tcgctattag gtcgtccctt ggatgctcct gtagatggtt tggtggatgc taatgcagtg
   112141 atggaacaat atgagaagtt gattgatttt gtcaaatctt gtccctatca tctggaaacg
   112201 gaaggatata tctttgtcca tgctggtgtg gacttgactt tgccagactg gcgaaaaact
   112261 agtaatcatg ataaggtttg gttacgtacc cctttccatg aggccgaaaa taccacaggg
   112321 aaaatgattg tctttgggca tacccctgtt tataacctct accagaccaa gctccgcatt
   112381 agccaaatct ggaccagttc agatgggaaa atggggattg acggaggagc tgtttacggc
   112441 ggagttctgc atggtctagt cttgaatcaa cagggattgg ttagagatta cgcagtgggc
   112501 tgtatggagt cgacccagta gcttactgta aaaatttaca aaattgcatg gtaatatata
   112561 ctagggatat taacaatttt taggagatga atatgccaat ttgttcggta taaaatttct
   112621 agtcaatccg gcagatttta tcaagcttta tccgagtgtt tagtcaggaa cggtatgaaa
   112681 taccctataa aaacctagtg attctcataa tgattctgca tttgagaggg ctactggttg
   112741 tatattctaa aaagataaat tgttttctag atatacttga ctttcatact taaaaataat
   112801 attatctagt agtaataaac aaaaggagtt ttataatgaa aaagaaatgg ttgctgattt
   112861 tctcagctct tctaacagtt actagtttgg tggcttgctc taatagtaag gaagaaacaa
   112921 cgtcaaaaag ctcttcaacg gaacaaacat ccacaacgaa atcctctact aaagagtcta
   112981 catctgaatc ctcgactgtt actttaaaag agtcaacagt gacttatctg gcagatgcag
   113041 aaattgatgc gattcagact atcggagaat ataaaacatc attgaactca ttgatggact
   113101 cttatgttgc tgactttgat gggttaattg ttcagttacc agaagaagca cagactactt
   113161 taaaaccata tcgtgagcaa attgtaacga tgcttgaaca acaaaaatca gcactagatt
   113221 cacaatttgc gacattaggg gatgactcga cagtgattcc agaagaagca cgagctactg
   113281 ttttggatag tttaaaaaca gttcgtgatc aactaaaaca aacgatgaca gttgtgcatg
   113341 aacaggcaca aacattatta aatgaataac agagaaagat ttggctcctg cccaatgtta
   113401 ttaagttagc taacttaata acattgggaa ctgagggcct cttttccatt tttttgcatt
   113461 tcttcccaaa ttttcagaaa attttgatat aatagaaaaa atattatttt tagattggaa
   113521 tcattatgac tgcacaaaaa atgactgcac aagaaatcat tgcctttatc ggtaatgcgg
   113581 tgaaaaagac aactgttaaa gtgacttttg aaggtgaatt ggctggtcct gttcctgcag
   113641 aagtgacaaa acttggcaac gtcctctttg gtgactggaa agacgtcgag ccacttttga
   113701 caaacttgac tgaaaatgta gactacgtgg ttgagcaaga cggccgcaat tcggctgtgc
   113761 ctttgctaga caaacgtaac atcaatgccc gtatcgagcc aggtgccatt atccgtgacc
   113821 aagtgacaat cggtgataat gccgttatca tgatgggtgc ggtcattaat atcggtgcgg
   113881 aaatcggccc aggtaccatg attgacatgg gtgctatcct tggtggtcgt gcgacggttg
   113941 gtaaaaacag ccacatcggt gcaggtgcgg tccttgcagg tgttatcgag ccagcttcgg
   114001 ctgagccagt gcgtgttggc gacaatgttt tggtcggtgc caatgccgtt gtcatcgagg
   114061 gagtacaaat cggtagtggc tctgttgtcg cagccggtgc tattgtgacc caagacgtcc
   114121 ctgaaaacgt agtggtggca ggtgtgccag cccgcatcat caaggaaatc gacgctcaaa
   114181 cccaacaaaa aacagccttg gaagaggctt tgcggacact ctaagaggta gctatgctag
   114241 atttaatcgc gacacgccgg gccctccacc agattccaga gctgggtatg gaggagttca
   114301 agacacacgc ctttcttatg gaaactattg agaacttgtt ggaagattgc tcttttgccc
   114361 aagtccgtac atggaagaca ggtatcctgg tctatctgac aggttctgct cctgaaaaga
   114421 ccatcggctg gcgggcagat attgacggcc tgcctatcgt ggaagaaacg gagctgggct
   114481 tcaagtccct ccacccaggc cggatgcacg cctgcggaca tgatttccac atgaccattg
   114541 ccctggggct tttggagaaa atggcagagc agcaaccgag aaataatctt ctcttcttgt
   114601 ttcagcctgc ggaggaaaat ctagcaggtg gtatgcttat gtatgaagcg ggggctttcg
   114661 gggattggtt gccagatgaa ttttatgggc tccatgtccg acctgacctc aaagtcggtc
   114721 aaatggccac caaccgtgcg accctctttg ctggaacctg tgaagtcaag atacggttta
   114781 caggaaaggg tggccacgca gcatttcccc ataccgccaa tgacgccttg gtggcagcca
   114841 gctactttgt gactcaggtg cagtcggtgg tcagccgtaa cgttgacccg atcgagggag
   114901 cggtggtgac cttcggctcc atgcatgctg gcacgaccaa caatgtcatt gctgaaacgg
   114961 cctttttgca cgggaccatt cgggccttga ctcagaacat gagcctcttg gtgcaaaaac
   115021 gggtgcggga agtggccgag ggaatcgccc tgtcctttgg agtggacttg gagatcgagc
   115081 ttaatcctag cggttatctg cctgtggaga acaatcccca gctggctgat gaactgatga
   115141 cctactttga tggtgttgac ggagtggaga tgattgattg tccacctgcc atgacgggag
   115201 aggacttcgg ctacctgctt aacaaggttc cgggtgttat gttctggttg ggtgtggata
   115261 cgccttatcc ccttcacaat ccacgtctca gtcccaaaga ggaagtgctt ccctttgctg
   115321 tggataagtt gagcgatttt ttgaaaatga aagcaaacta gactggagtt tccagtcttt
   115381 ttctgaaaag aaaggagttc ctgtggataa aaaagaaatt cgacaagagg tcttgcagag
   115441 attgaaagcc ctgactagta gccagcgagc aagatggagt cagcaactaa cagaaaggct
   115501 cctttcatct gatacctata agtgctcaaa gtcgcttgga acctaccttt ccatgcccca
   115561 tgagtttgac acttcttacc tgattgagca ggctcaaaag gatggcaagc agattttcat
   115621 ccctaagacc tattcacaag gtcgtatgga ttttgtagaa tataatcctg atgatttgat
   115681 gaaatctagg tttggagttt gggaacctgg gacttattca caacctgtgg ataagtctgt
   115741 ggttaagttg atccatgttc ctggtttagc ttggaatcaa gcgggttttc gagtgggtta
   115801 tggaggcggt ttttatgatc gctacctagc agattatcaa ggggagacgg tatcgacctt
   115861 ggcagacttt caagtctatg attttgaacc agatgtattt gatattccag taaaggagtt
   115921 gttgattttt gaagaacttt tttgataagc gctatcctgt gaccaatgcc ttgttggcag
   115981 tcacggctct tgtttttctt ttgatacaaa tatttcgatt tgggcagacc actacagcct
   116041 atacgatttt tgaattcggt ggtatgtacg gtcaagtggt gcggtatgat ccgactcagt
   116101 tgtggcgctt gatttctcct atctttgtcc acattggttg ggaacatttt atctttaaca
   116161 gcattacctt gcttggtttg ggttatcaat tagaaggact gtttgggtca cggcgcttct
   116221 ttctcctcta tctcttgtct gggataatgg gcaatctttt tgttctcttc tttacaccag
   116281 atgtagttgg tgcaggtgca tcgacctctc tgtttggtct ttttgcagct atggcacttt
   116341 tgaggaaatt tagtcgcagc ccttatctac aggttctcgg tcaacgttat atggttcttt
   116401 tgggattgaa cttggtcttg ggacttttta atccaaccat tagcatggca ggacatattg
   116461 gaggagctat tggtggttgt ttggtcgtga ttttccttcc ccctcttgtg gaaaaggatc
   116521 tctttacagg aaaacagatt tttttcagct tcattggcta tatgagcttg atcgtttttc
   116581 tactagggat attctatttg atataagaaa aaaactagcg attgctagtt tttctttgta
   116641 tctaattttt taccaagatc gattaagtat tgtttcaaat catcttttac ttgagggtgt
   116701 tggagggcgt agtcgataga agttttcatg aaaccgaact tatccccaac atcgtaacgg
   116761 ttgcctttga attcacgagc gaaaacacgt tgcgttttgt tcagagtatc aatggcatct
   116821 gtcaattgaa tctcgttccc ggcacctggt ttttggtttt ctaaaatctc aaagatttca
   116881 ggagtgagta agtagcgtcc gataatagca aggtcagaag gagcgtcttc tggttttggt
   116941 ttttccacaa aggtttccac actatagaga ccgttgacac cttcaccttg tggagcgata
   117001 acaccgtatg cagaaacttc ttcatgagga acaggcataa cagcaatggt tgaagcatgt
   117061 gttttttcat agtcgttcat gagttgcttg gtcaatggga cagcttttgt atctgtgatg
   117121 tccatcaagt catcgccgag catgacaaca aatggctcat ttccgacaaa ggcttttgct
   117181 tgaagaacag catccccaag tccacgtgga tggctttgac gaatgaagtg tagtcgaata
   117241 cctgttgtct catcaaccag tttcaacaaa tcatttttgc ctttttcttt gaggttgtat
   117301 tccaattcaa agtttgaatc aaagtggtct tcgatagaac gttttgactt accagtgacc
   117361 accaaaatat cttcaatacc tgaacggagg gcttcttcaa cgataaattg gatggttggc
   117421 ttgtcaacga ttggcaacat ttccttggca agagccttag tagcaggcaa gaaacgtgtt
   117481 cccaaacctg cagctgggat aacagctttt ctaacttttt tcataagtat gtaatccttt
   117541 ataatttatt tttttgtgtt taataagaat caatgccatt cattttcatg gcggaattca
   117601 cccgtcatga tttctttgat ggcatcttca atacctgcac cttggtagat gaccttgtag
   117661 atggcttggg taataggcat gtaaacatct aattcttgag ccaactcata ggctgcctta
   117721 gttgttgaaa taccttcaat caccattccc atattgcgtt ctacatcttc gagtttttca
   117781 ccgcgaccga gctgatctcc agctctccag ttgcgggagt ggacagatgt accggtaaca
   117841 atcaaatcac caacaccaga aagaccgctg taagtcagag gatttgcgcc catagcaacg
   117901 cctagacgtg tgatttctgt caaaccgcga gcgataatag ctgccttggc attatcacca
   117961 taaccaagtc cgtgtaaagc gccagcgccg acagcaataa tatttttaag ggcacctgct
   118021 gtttctacac cgatgacatc attatttgta taaagtcgga agtagtggtt gctgaaaaga
   118081 ttttggacgt agctggctgt ttccaagtct tttgaagctg cagaaatcaa ggttaaatca
   118141 cggacgatag tctcttctgc atgacttggc cctgaaacca cgacgatttc agaacggagt
   118201 tcagcaggaa tttcttcctc cagtacctca gacaagcgct tgtgactatc aggctctaag
   118261 ccttttgaag cgtgcatgac aactaccttg tgtttgagag cttgtgcgac ttgcttggca
   118321 acgagacgtg ttaccttggt tggtacaacg aaaagaatag cgtcaacacc gtccaaggct
   118381 tcagtcaaat ccttacagcc cttgatgttt tcatctagga taacatcctt gaaatagcgc
   118441 ttatttgtat gttctttatt gatttcgtcg atttgttcgg ggacatttcc ccaaatacgg
   118501 acagtatgac cgttgtcgtt gagaacttgt cctagggcgg tgccccatga accgggtccc
   118561 aagatggcaa tggtttgttt ggtcataaaa aatcctttct agttggtgta atttagatat
   118621 attataccat aat
//
LOCUS       DAZQCJ010000002        65384 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000002 DAZQCJ010000000
VERSION     DAZQCJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 65384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65384
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.002"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            124..897
                     /locus_tag="ACHU66_000113"
     CDS             124..897
                     /locus_tag="ACHU66_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFR3388430.1"
                     /translation="MILKEQMLLVDQVQLAPRIFAMTLQGDMVQDMKMGQFIHIRVPD
                     DSMLLRRPISISEIDRNKSQCRIIYRVEGQGTEVFSKMTVGQYLDVMGPLGKGFEVDF
                     LKEGDRILIIGGGIGVPPLVEVAKRAAFRGVNVTSVIGFATKEAVILEEELASYGMVY
                     VTTDDGSYGRKGNVATVVEELTNEFAAIYSCGAPAMMNYVDQRFQEHPHAYISLEARM
                     ACGMGACYACVVKPKEGQEHENKRVCKEGPVFATGSLIL"
     gene            907..1848
                     /locus_tag="ACHU66_000114"
     CDS             907..1848
                     /locus_tag="ACHU66_000114"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFR3388431.1"
                     /translation="MIKRLAISLPGLDLKNPIIPASGCFGFGQEYADYYDLNQLGSIM
                     IKATTRHPRYGNATPRVAETPAGMLNAIGLQNPGVDVVLSEKLPWLEQHFPDLPIIAN
                     VAGFSNEEYAYVSGKISKAPNVKAIELNISCPNVDHGNNGLLIGQVPELAYQAVKAAV
                     EASSVPVYVKLTPSVADITLLAKAAEDAGATGLTMINTLVGMRFNLKTRQPILANGTG
                     GMSGPAVFPVALKLIRQVAQVTDLPIIGMGGVDTADKAIEMMVAGASAVGVGTANFTD
                     PFACPKIIQDLPKRMEVYGIDSLEGLRREIRHRLGKR"
     gene            2013..2708
                     /gene="pyrF"
                     /locus_tag="ACHU66_000115"
     CDS             2013..2708
                     /gene="pyrF"
                     /locus_tag="ACHU66_000115"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR3388432.1"
                     /translation="MKETRPIIALDFAGKEEVQSFLEQFPPDEKLYVKVGMELYYAEG
                     PGIINYLKGCGHSIFLDLKLHDIPNTVESAMKVLAKLGVDMTNVHAAGGVEMMRAARR
                     GLGKDAVLIAVTQLTSTSEEQMRTDQNIQTSLQEAVVHYAQRAQEAGLDGVVCSAHEV
                     ALIKDATSSDFVCLTPGIRPTGGEVGDQKRVMTPADAARIGSDYIVVGRPITKSEHPY
                     QTYLSIKEEWNRG"
     gene            2701..3027
                     /locus_tag="ACHU66_000116"
     CDS             2701..3027
                     /locus_tag="ACHU66_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR3388433.1"
                     /translation="MDKIYQVIIDRPIGYIDKFGNQYPINYGYVPNLLAGDGEEQDVY
                     IISKAVRQPLTAFEGKLVAIIHRRDDVEDKWVLTGLDEKLTVTEIKEKTNFIEQYFDS
                     WIEMVD"
     gene            3164..3796
                     /gene="pyrE"
                     /locus_tag="ACHU66_000117"
     CDS             3164..3796
                     /gene="pyrE"
                     /locus_tag="ACHU66_000117"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR3388434.1"
                     /translation="MTLATEIASHLLDIKAVHLRPEKPFTWASGIKSPIYTDNRITLS
                     YPETRNLIEDGFVQRIEEEFPEVEVIAGTATAGIPHGAIIADRMNLPFAYIRSKPKDH
                     GAGNQLEGRIVKGQKMVVVEDLISTGGSVLDAVAAAEREGADVIGVVAIFTYELPKAD
                     CNFDNAGVKLVTLSNYSELIKVAKVQGYINADGLNLLKKFRQNQETWQED"
     gene            3905..4831
                     /locus_tag="ACHU66_000118"
     CDS             3905..4831
                     /locus_tag="ACHU66_000118"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR3388435.1"
                     /translation="MISVIVPCFNEEEAIPYFYDAMEKVRKEMGEQFEYIFVNDGSKD
                     RTLTVLRQLSGQDRAVRYLSFSRNFGKEAALYAGLQAAQGELVTVMDVDLQDPPEMLM
                     EMKAMLDGNPDLDCVGTRRVSRDGEPPIRSFFAKLFYKLMNKISQVEVVDGARDFRLM
                     RRHMVDAILSVSEYNRFSKVIFAWVGFETEYLPYENVERVAGETSWSFWKLLSYSIEG
                     IINFSDTPLNIASYTGFFTFLLSLVLMVFVVFKTLVFGDPTIGWPSTICIILFLGGLQ
                     LMTIGILGKYLAKVFLETKKRPIYIVKEKSSN"
     gene            4841..5494
                     /locus_tag="ACHU66_000119"
     CDS             4841..5494
                     /locus_tag="ACHU66_000119"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR3388436.1"
                     /translation="MEHSAWHELIKAQLPEHYFGKINQFLDYVYAGGTVYPPREKVFA
                     AIQTTDLEEVKVVILGQDPYHGPGQAQGLSFSVPNSVPAPPSLQNILKELTDDIGVKQ
                     DHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIKVVNSLDQPVVYILWG
                     AYARKKKALITNPKHLVIESAHPSPLSAYRGFFGGKPFSQANAYLTSQGRTGIDWLR"
     gene            5534..6805
                     /locus_tag="ACHU66_000120"
     CDS             5534..6805
                     /locus_tag="ACHU66_000120"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR3388437.1"
                     /translation="MLLVKNGRVLDPQSGLDKVCDILMDGSEIVKIAESISEDVEQEV
                     IDATGLVVAPGLVDVHVHFREPGQTHKEDIHTGALSAAAGGFTSVVMMANTNPTISNV
                     ETLKEVLDSAKKEAIHIYSVATITDHFDGQNLTDFPALLEAGAVAFSDDGIPLTNAGV
                     VRKAMKLAKEQGCLISLHEEDPDLNGVLGLNEQVAEKHFHVCGATGVAEYSMVARDVM
                     IAYETGARVHIQHLSKAESVKVVEFVQGLGANVTAEVAPQHFSRTEDLLLIKGANAKM
                     NPPLRLESDRLAVIDGLKSGVISVIATDHAPHHADEKNVEDLTQAPSGMTGLETSLSL
                     GLTHLVEDGHLTLLQLLEKMTINPAKLYGLDAGYLAENGPADLVIFDPNANRLVTADF
                     ASKSANSPFVGDSLKGQVLYTIANGQIVFGK"
     gene            6936..9050
                     /gene="nt5e"
                     /locus_tag="ACHU66_000121"
     CDS             6936..9050
                     /gene="nt5e"
                     /locus_tag="ACHU66_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface ecto-5'-nucleotidase Nt5e"
                     /protein_id="HFR3388438.1"
                     /translation="MKKKKILLPVMSTLLLAPFVLAQQVQAAETTTAATTTNQPATTD
                     ATATVPATSVENVATEETVVPAAEETVEAVIIHTNDVHGRILEEKNVIGDAKAAAVIE
                     EERAKVENTIVVDAGDAFQGLPISNSTKGEDRANIMNQVGYDAMAVGNHEFDFGMDQA
                     IKYKETLNFPLLSANTYVNGARVFEASTIVDKTPTVVGDEFVVIGVTTPETATKTHPK
                     NVEGVTFTDPVTEVNKVIDEVEARALADNRVYKNYIILAHLGVDSTTPVEWRGSTLAE
                     ALSKNSKLAGKRVIVIDGHSHTVEATTYGDNVTYNQTGSYLNNIGKVTLKSDKLLGEA
                     SLISAADTKNVTPNAKIAALVDEIKAKYEAENAQVVIENNPVELNGDRSNVRVRETNL
                     GNAVTDAIYAYGQTGFSNKTSLAVTNGGGLRATIAKDQPVTKGDIIAVLPFGNIVSQI
                     TVTGQQIYDMFTKSLSSTLQVNPETGEMLLDENGMPLFEASGGFLHISGANVFYDPTL
                     PVEERVLLIGILNPETGEYDALDLEKTYYLATNDFLAAGGDGYTMLGGAREEGPSMDS
                     VFAEYLKTADLSAYEVVNPYSRIIPVNSSIDTDEDGYPDFIEILLDTDPENPASNPET
                     VPAENTDSPSNQVQNTSATDKKAPVDSPKVGDKKTEVASPVKTTKAGVLPNTGDQMNL
                     TLSLFGLGLAGLAMAVGRRKEN"
     gene            9102..9749
                     /locus_tag="ACHU66_000122"
     CDS             9102..9749
                     /locus_tag="ACHU66_000122"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFR3388439.1"
                     /translation="MQTVFQYQKEGDREQISSYVLLSNSPRRKQLLGFLQPQIASVEV
                     DERGIEEHFMKQFATDDFLTRAAKTCCEISKAKSDMDLVTGHLYISADTIVVSDGQIF
                     NKPQDLTEAEIMLRSYFGKSHHVVTSVCLRTRHSLDVFYTVAQIDFVDYYPALEEVIQ
                     SYIREKQPLDKAGAYGIQELDPRFVSGISGDIHTIIGLPVAEVSRRIFSEEGFEK"
     gene            9801..10289
                     /locus_tag="ACHU66_000123"
     CDS             9801..10289
                     /locus_tag="ACHU66_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR3388440.1"
                     /translation="MTVDIERYKQLAQQKQGEHRKFLANLKKKAPKNLDRIVQDIHAE
                     VFSEIDCTECVNCCKTLGPLFTEADITRIAKHLRMKLPVFEDMYLRVDEDGDKVFQSM
                     PCPFLGGDNLCSIYDARPKACREFPHTDRKKIYQINNLTIKNTLICPAAYLFVEKLRE
                     RI"
     gene            10389..11240
                     /gene="ylqF"
                     /locus_tag="ACHU66_000124"
     CDS             10389..11240
                     /gene="ylqF"
                     /locus_tag="ACHU66_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002962311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFR3388441.1"
                     /translation="MATIQWFPGHMSKARRQVQENIKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKVDLADPVRTKEWQSYFEGQGIRTLAINSKEQATVKKVTDAAK
                     SLMKDKIERLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKDLEILDTPGILWPKFEDQEVGLKLALTGAIKDQLLPMDEVTIFGLDYFKANY
                     PERLQERFKGMDLEQETPELIMDWTKKLGFRDDYDRFYNLFVKDVRDGRLGTYTLDRV
                     SDLDGND"
     gene            11227..12006
                     /locus_tag="ACHU66_000125"
     CDS             11227..12006
                     /locus_tag="ACHU66_000125"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR3388442.1"
                     /translation="MATIKEVTTLLAQVDSLDSPVWAELSQDERAGVQAAIKKRRKEL
                     DKEVAEDARLEAMLFYEKNLYENGVEFIAGIDEVGRGPLAGPVVAAAVILPKGCKIRY
                     LNDSKKIPKSKHEAIYQEVMERAVAVGVGIKDAAVIDQVNIYEATKLAMLEAFGKLSQ
                     KPDHLLIDAMKLDTPIPQTSIIKGDANSLSIAAASIVAKVTRDKMMADYDKEFSGYGF
                     AKNAGYGTAEHLEGLNKLGITPIHRKTFEPIKSMVTGGKSS"
     gene            12003..13013
                     /locus_tag="ACHU66_000126"
     CDS             12003..13013
                     /locus_tag="ACHU66_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFR3388443.1"
                     /translation="MTVFFWIFLASIGLFGLLYFWERRSIFISLFALNILVWALAWII
                     STGILENNEILRLLAIAVAMVLFLAFLSGPFVLLFTLYLNGFQILEREGVRFHNFLSM
                     GLAVALTFYLFIAPFVVQSLSGISLLNMVFVYVGFLVSYAIIISMLYTTSSFVNFVNL
                     FPGKLDYVVVLGAGLIGDKVTPLLASRIDKGIAIYQKQPGSKLIMSGGQGPDELIAEG
                     QAMANYALEQGIPAEDIIIENQSTNTEENLKFSYALMKPGSRFALVTNYYHVFRALLL
                     ARQLKIKCIGYGAKTKFYFSLNAFIREFVGYVVMSRKTHLVFLSIVSALYLIGVIISL
                     MH"
     gene            complement(13046..13696)
                     /locus_tag="ACHU66_000127"
     CDS             complement(13046..13696)
                     /locus_tag="ACHU66_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR3388444.1"
                     /translation="MNPSLKLSLQLSFGEEVANSVTHAVGALLMLVLLPVTAIHSYQD
                     YNLTAVIGMSIFVISLFLMFLSSSIYHSMPYASTHKYILRIIDHSMIYIAIAGSYTPV
                     ALSLVGGWLGYLIVGLQWGLTIFGILYKIFAKKINEKFSLFLYLFMGWLVVFILPAIL
                     QKTGMAFGILMLAGGLSYTIGALFYAKKKPYFHMIWHLFILLASALQYIAIVYFML"
     gene            complement(13686..14237)
                     /locus_tag="ACHU66_000128"
     CDS             complement(13686..14237)
                     /locus_tag="ACHU66_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFR3388445.1"
                     /translation="MKQTIPYWHELPNLDLYLDQVLLYVNQVVNSQESLEQKSLYSMK
                     INSSLGNETDDRTGVHQGKLTTDNVDFRRVLTAAMINNYVKHKQIEKPVKKKYQKQQV
                     ARLIALTILKNVFSIQEISQTLHLLLQSSHSETLYNHFVDCMRNEENEETPDIIRYAC
                     QSVKLYYKTRQLTMELERSHHES"
     gene            complement(14345..15997)
                     /locus_tag="ACHU66_000129"
     CDS             complement(14345..15997)
                     /locus_tag="ACHU66_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR3388446.1"
                     /translation="MKIIIVGGVAGGMSAATRLRRLMEDAEIIVFEKGPYVSFANCGL
                     PYYISGEIAEHDSLLLQTPESLKQRFNLDVRPFHEVISIDAEQQTVTVKTPTGELIES
                     YDKLILSPGAKPVIPNLSGIEEANNLFTLRNVPDLDKIMIKLDQIKSGHVVVIGAGFI
                     GLEMAENLALKGWKVTLVEQAPHVLPTFDLEMARYVEAELTNKGIEVITGQSVTAFHE
                     KGQVLELSNGQTLSSDVTILSVGVAPDSQLAKKTGIELGLRGGILVDKNYQTSQNNIY
                     AVGDAIVVKQEITGQDALISLASPANRQGRQVADVIAGLDRKNKGSIGTAIVKVFDLA
                     AASTGLSERTVQSLGYEHAIIHTTGKDHAGYYPGATDIVLKLIFSPTDGKIFGAQAVG
                     QKGIDKRIDVLATAIKAGLTIFDLPELELTYAPPFGSAKDPVNMIGYAAMNITEGISQ
                     NIQWHQLDDELATGKVLLDVRNPGEVANGQFREFIHIPLDQLRERIGELDASKEYIVI
                     CHSGLRSYIAERILKEKGFGVKNLDGAFSLYKMALPENIIQP"
     gene            complement(15994..16317)
                     /locus_tag="ACHU66_000130"
     CDS             complement(15994..16317)
                     /locus_tag="ACHU66_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR3388447.1"
                     /translation="MFKQLFSGMFSNSDSLPITDVEQAIQSSNAILLDVREVDEFASG
                     HIPQAINQPLSQLDQFKGDKTKNYLVICQSGMRSQRATEYLKSQGYQAINIKGGMNAW
                     TGGIA"
     gene            16436..16696
                     /locus_tag="ACHU66_000131"
     CDS             16436..16696
                     /locus_tag="ACHU66_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFR3388448.1"
                     /translation="MNTDKKKMINRLKRAEGQLRGIQKMIEEDQECIDIVTQLSAVRS
                     SINSMMGMVIAQKVQDCITHPSENPEEQEARLAQAINLIIKK"
     gene            16707..17264
                     /locus_tag="ACHU66_000132"
     CDS             16707..17264
                     /locus_tag="ACHU66_000132"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HFR3388449.1"
                     /translation="MPTEKEKMIAGQFYMAADAELREMRTFARQQMKKFNEELDGQAR
                     SERLKNWLGATGERIYVEPNFYCDYGSNIHVGEDFYANFNCTMLDVCPITIGKNAMLG
                     PNVQLLTPLHPLDPDERNSGLEYGSPITIGDNFWAGGGVTILPGVTLGNNVVVGAGSV
                     VTKSFGDNVVLAGNPARVIKEIPKK"
     gene            17323..18165
                     /gene="dprA"
                     /locus_tag="ACHU66_000133"
     CDS             17323..18165
                     /gene="dprA"
                     /locus_tag="ACHU66_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFR3388450.1"
                     /translation="MNNFELFKWKKAGLTNLNVNKVLAYQEKHGKKLTLRNVAVVSEC
                     KNPVLFMENYKNLDTKACREEFNKYPSFSILDDIYPLALKQIYNPPVLLFYQGDLELL
                     EKSKLAVVGTRNASKNGVQSVQKIIKELNNRFVIVSGLARGIDTAAHIASLKNGGQTI
                     AVIGTGLDKIYPKENRELQTYIGKHHLVLTEYGPGTDPLKYHFPERNRIIAGLVEGLV
                     VCEAKMRSGSLITCERALEEGREVYAIPGSIMDGKSDGCHHLIQEGAKCVTSGLDIIS
                     EYQF"
     gene            18258..20351
                     /gene="topA"
                     /locus_tag="ACHU66_000134"
     CDS             18258..20351
                     /gene="topA"
                     /locus_tag="ACHU66_000134"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR3388451.1"
                     /translation="MATKTVSKKKTTTKKNLVIVESPAKAKTIEKYLGKNYKVVASVG
                     HIRDLKKSSMSIDFDNNYAPEYINIRGKGPLINSLKKEAKNAKQVFLASDPDREGEAI
                     SWHLAHILGLDANDKNRVVFNEITKDAVKNAFQEPRAINLDLVDAQQARRVLDRIVGY
                     SISPILWKKVKKSLSAGRVQSVALKLIIDRENEISNFKPEEYWTIDATFKKGSKKFQA
                     SFYGIDGKKTKIENNEQVKEILARLDGDDFLVEQVERKERKRNAPLPYTTSTMQMDAA
                     NKINFRTRKTMMVAQQLYEGVSLGTGGTQGLITYMRTDSTRISPVAQAQAADFITERF
                     GATYSKHGNKVKNASGAQDAHEAIRPSNVNLTPESIAKYLDKDQLRLYTLIWNRFVAS
                     QMAVAVFDTMSVRLGQNGIVFAANGSQVKFDGYMAVYNDADKNKMLPDMEQGDTVVRA
                     STNPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVKLAAKRFEPT
                     ELGEIVNKLIVEFFPDIVNVKFTADMEQKLDDVEIGKEQWQKVIDGFYQPFKVELSKA
                     EEQMEKIQIKDEPAGFDCDVCGHEMVIKLGRFGKFYACSNFPDCRNTKQITKEIGVTC
                     PVCQKGQVIERKSKRNRLFYGCDRYPECEFTSWDKPVGRSCPKCDHYLVEKKVRGGGK
                     QVVCPNGDYEEDKIK"
     misc_feature    20767..20901
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            20928..21422
                     /gene="rplJ"
                     /locus_tag="ACHU66_000135"
     CDS             20928..21422
                     /gene="rplJ"
                     /locus_tag="ACHU66_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR3388452.1"
                     /translation="MSEAIIAKKAELVNAVAEQMKAATSIVVVDARGLTVEQDTVLRR
                     NLRGAEVEYKVIKNSILRRAAEQAGLEGLAELFVGPSAVAFSNDAVAPAKTIYDFAKT
                     ADALEIKGGAVEGKVSSKEEIEALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAEK
                     EDAA"
     gene            21488..21853
                     /gene="rplL"
                     /locus_tag="ACHU66_000136"
     CDS             21488..21853
                     /gene="rplL"
                     /locus_tag="ACHU66_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR3388453.1"
                     /translation="MALNIENIIAEIKEATILELNDLVKAIEEEFGVTAAAPVAVAAA
                     GGAAEEAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPAMVKEGVATA
                     EAEEIKAKLEEAGASVTLK"
     gene            22053..22220
                     /locus_tag="ACHU66_000137"
     CDS             22053..22220
                     /locus_tag="ACHU66_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388454.1"
                     /translation="MQKKEQSSRQIVMCHLVAILGVDIEKATHLVDELEQVGLIRFDE
                     FGNVGLLVLEG"
     gene            22223..23038
                     /locus_tag="ACHU66_000138"
     CDS             22223..23038
                     /locus_tag="ACHU66_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFR3388455.1"
                     /translation="MKRITANQYQTSERYYKLPKVLFESERYKDMKLEVKVAYAVLKD
                     RLELSLSKGWIDEDGAIYLIYSNSNLMALLGCSKSKLLSIKKTLREYGLIDEVQQSSS
                     ERGRMANKIYLGELEHETTPVLHTDGASVKKTLGGSQRKTGPVLYSAPSETEGSETKY
                     SETKGSDFLIEDEEERQLVDEKQEENFTSKVDGVTKYDRDYIWGLVHDQLRQTGLSQS
                     ASDYAMLYFSDRYQYALEHMRFARSAEVIAEYVFNGVLSEWTKQLRRQEVKGG"
     gene            23190..24545
                     /locus_tag="ACHU66_000139"
     CDS             23190..24545
                     /locus_tag="ACHU66_000139"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFR3388456.1"
                     /translation="MKFLDLFAGIGGFRLGMESQGHKCLGFCEIDKFARTSYKAMFNT
                     EGEIEYHDIKEVTDHDFRQFRGQVDIICGGFPCQAFSLAGRRLGFEDTRGTLFFEIAR
                     AAKQIQPRFLFLENVKGLLNHDEGRTFATILSTMDELGYDVEWQVLNSKDFQVPQNRE
                     RVFIIGHSRRYRSRFIFPLRRENSPAHLERLGNINPSKCGLNGEVYLTSGLAPTLTRG
                     KGEGAKIAIPVLTPDRLEKRQHGRRFKDNQDPMFTLTSQDRHGVVVAGNLPTSFDQTG
                     RVFDISGLSPTLTTMQGGDKVPKILLREELPFLKIKEATKTGYAKATLGDSVNLAYPD
                     STKRRGRVGKGISNTLTTSDNMGVVVAALEYRQDKWYEVTGIVLEGKVYRLRTRRLTP
                     RECFRLQGFPDWAYERAESVSSKSQLYKQAGNSVTVTVIEAIAREFRRTEEEEKHELT
                     T"
     gene            24529..24957
                     /locus_tag="ACHU66_000140"
     CDS             24529..24957
                     /locus_tag="ACHU66_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388457.1"
                     /translation="MNLLHKKSILDCTELEERIHQAETNQLLQKILSLPNFDCDFEVT
                     FEDDYHKEMNDPLFYESNLHQISDFMETRDIKNGVDTLLTKDNHLAFRAFGENYSARG
                     KDGILTTLVIVKCFGEGRMPIDMSRYFSTPEPTVENSLIL"
     gene            24968..25351
                     /locus_tag="ACHU66_000141"
     CDS             24968..25351
                     /locus_tag="ACHU66_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388458.1"
                     /translation="MLEVYLGNNANTNQDLLTILTTYGVTYRCIKACDVDREILLSLF
                     AKTMDCFELLSPRFLCFKRQYSISLNEMIQLILQKPDQNLRLPLIVCQNHVYPAIGLD
                     EVRTFLPRQVKEELFQASLMKQVTG"
     gene            25360..25593
                     /locus_tag="ACHU66_000142"
     CDS             25360..25593
                     /locus_tag="ACHU66_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR3388459.1"
                     /translation="MNERFWDNLEIILAEKELTWAELARKVFNGQYVYPSEFNRLYQK
                     LRHYKSNRLMPQTRWVERIVLVLEIDYEDLFKR"
     gene            25596..26180
                     /locus_tag="ACHU66_000143"
     CDS             25596..26180
                     /locus_tag="ACHU66_000143"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HFR3388460.1"
                     /translation="MIRIVVFYLAIQLNGLLVSLYLKEYLTIEGIVLLQLVLLSVTCL
                     EIARHKTVQAKNITLRNRLRWLLLGFVAMVAFAVFISFLFPGQTRNQAVLVQVGKQVP
                     PIIFLLFLVNASILEEIVYRQLLWEKLTFPFVQIGVTSFLFVLSHGPNQLGSWLMYSC
                     LGLTLAAVRLKADCMTAIALHLLWNSLAYVVTFL"
     gene            26258..26746
                     /locus_tag="ACHU66_000144"
     CDS             26258..26746
                     /locus_tag="ACHU66_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388461.1"
                     /translation="MSSEQQERQAMQYVERSSLLTVRTLLKLLEWSARQALAQDSAYK
                     IGVQKLEELLQSPYVIEAIDVSKDILDKPIDVEKFKELMESEKLPIAISWQKDYLYFY
                     AKDKTLLDHHLDELLKKLMSNPEKLEGLTFDKTLDQEIELAKEKIRVTEPSAVKTKEV
                     TL"
     gene            26746..28563
                     /locus_tag="ACHU66_000145"
     CDS             26746..28563
                     /locus_tag="ACHU66_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFR3388462.1"
                     /translation="MYSRRKALVFGLLGLAFGYFCHRLTLLYDSLTNAPPMERFAYLL
                     GEGLNQVFNPLWLFSFTQKSLLAFILGVLTMTLVYLYVSTGQKVYREGEEYGSARFGT
                     SKEKWNFYSKNPLNDTILARDVRLTLLEKKKPQFDRNKNLIVIGGSGAGKTFRFVKPN
                     LIQLNCSNIVVDPKDHLAEKTGKLFLENGYQVKVLDLVNMTNSDGFNPFRYVETENDL
                     NRMLTVYFNNTKGNGSRSDPFWDEASMTLVRAIASYLVDFYNPPGSSKQEQEARRKRG
                     RYPAFSEIGKLIKLLSKGDNQDKSVLEVLFEDYAKKYGHENFTMRNWADFQNYKDKTL
                     DSVIAVTTAKFALFNIQSVIDLTQRDTMDLKTWGTQKTMVYLVIPDNDTTFRFLSALF
                     FSTVFSTLTRQADVDFKGQLPIHVRSYLDEFANVGEIPDFAEQTSTVRSRNMSLVPIL
                     QNIAQLQGLYKEKEAWKTILGNCDSLLYLGGNDEETFKFMSGLLGKQTIDVRSTSRSF
                     GQTGSSSTSHQKIARDLMTADEVGNMKRDECLVRIAGVPVFRTKKYFPPKHKNWKWLA
                     DKETDERWWHYRINPLTAEEELELSGHKIRDLSTETTLH"
     gene            28581..28823
                     /locus_tag="ACHU66_000146"
     CDS             28581..28823
                     /locus_tag="ACHU66_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388463.1"
                     /translation="MNQKLSGFVYGVDASSMFSQAMSLLQKGLIAVGAFLVVMGIINL
                     STNIKDGGPGVRNAILEIVGGVMVGAAGTFVTQITI"
     gene            28842..29696
                     /locus_tag="ACHU66_000147"
     CDS             28842..29696
                     /locus_tag="ACHU66_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL"
                     /protein_id="HFR3388464.1"
                     /translation="MIMNFTSSFVYLASEKVSSDSLFEGFQVDLESTANLVKSLADFN
                     PTVWSYMTAITKGIMQPLGVAILAVVLVLEFSKMAKKIANSGGAMTFEAMAPMIVSYI
                     MVAVVITNTTVIVEAIIAIASYVIEQVAELVTNGGANYDTISGIKGSGIVGKMIIGFF
                     AILIWLVRMASIMVVNILITIRFIQLYLMIPFAPVTIPTFLSDDWRSVGIGYLKNIMV
                     YAVQGILIFLIVSLVPLFESAGKIAVSNGAGVMESLAIMFGGLVQAILLIIALVGSQR
                     TARSILGM"
     gene            29758..30111
                     /locus_tag="ACHU66_000148"
     CDS             29758..30111
                     /locus_tag="ACHU66_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFR3388465.1"
                     /translation="MNTRVFKDISKVQHRAWLGFTTRQVIFVFPAVALTILVLGLNLF
                     YWQFGDWFVYGFVFSFTIPLMLFGVYRPNDLPFETYLKYRFHYELTVPDRTFTGRKGD
                     QREKIKTLNETKDLF"
     gene            30068..32419
                     /locus_tag="ACHU66_000149"
     CDS             30068..32419
                     /locus_tag="ACHU66_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFR3388466.1"
                     /translation="MKKLKHLMKPKTSSNDKKQKTKTQKQEIRPSTVNTLAYQGLFQN
                     GLMQVSPSYFSQTYLLGDVNYQTVGLDDKGAIVEKYSDLINSLDDKTNFQLTILNQKV
                     NLEKFRKSILYPLQEDGFDAYRDELNRMMNANLEAGENNFSAVKFLSFGKSDQTPKLA
                     FRSLSQIGEYFKSGFSEIDVSLGLLGGEERVNVLADMLRGENHLPFSYKDLTLSGQST
                     KHFIAPTYLSFKHKNHIELDDRLLQIVYVRDYGMELGDKFIRDLMQSDLEVMISLHAK
                     GSTKSETMTKLRTKKTLMESQKIGEQQKMARTGIYLEKVGHVLENNIDEAEALLQTMT
                     QTGDKLFDTVFLIGVLADTEDQLKQSLDIIKQVAGSNDMIIDNLTYMQEAAFNSLLPF
                     GKNYLEGISRSLLTSNIAVNAPWTSVDIQDKGGKFYGINQISSNIISIDRGKLNTPSG
                     LILGTSGAGKGMATKHEIISTKLKEADSDTEIIIVDPENEYSIIGQAFGGESIDIAPD
                     STTFLNVLDLSDENMDEDPVKVKSEFLLSWIGKLLDRKMDGREKSLIDRVTRLTYKHF
                     DTPSLVEWVFVLAQQPEQEAKDLALDMELYVEGSLDIFSHRTNIKTDSHFLIYNVKKL
                     GDELKQIALMVIFDQIWNRVVKNQKLGKKTWIYFDEMQLLLLDKYASDFFFKLWSRVR
                     KYGAIPTGITQNVETLLLDANGRRIIANSEFMILLKQAKSDREELVHMLGLSKELEKY
                     LVNPEKGAGLIKAGSTVVPFKNKIPQHTKLFDIMSTDPEKMRT"
     gene            32421..35222
                     /locus_tag="ACHU66_000150"
     CDS             32421..35222
                     /locus_tag="ACHU66_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFR3388467.1"
                     /translation="MKEDKKLVKHARQNFRSNLKSARMHYRKEVRALKQTVPKKGRFR
                     KPAQNSLFQEKKTEIKGNLLSSQKEAEEKFLKEITYVSPRLLKVKEIKNYRLPQAQER
                     LRTARKHLSEVKLSEKQKAVNPKFTFQKENPSPKSRFQFHQEKSFDRLSAEKDVSSAK
                     REVKQLKKVQKTKKNSTKVKVGLGLVASESLDLIAQEDDLDGLRSMKDISLKARRYGR
                     FTFQAGKVVVKSGQTGVRFTKTKFSHGKERFQNFKKGKGFTRQKPLKPRRRYQTFLKH
                     VRKQSVAGIKGIVQAIKGSVTFFSVLAGNPLTWIVSGILLILLLMMSFFMSVSGSSVI
                     QQDEMELTKAYTHMTWEDAEHTRTNEKGIAYYTKIDEIMVYMNHQYQDYKLDDFMETG
                     GVTYKAFLSQVWTDLNGGDSIKSMSDLYKEPTYKLSDEDQEELKELTEGGNYLALQEL
                     DNPFQGQTDEDSLSMTYRYGYEVIDEKPTLHHHIILEAKEGQVIVAPMDGKVSLDGEN
                     VVLTSGKGVNKTKLTLFGIHSGRVSEHQHVLAGDIIGQTKDGTGLKVTYQKVDDDTDK
                     LVYVNPAFYFPKVIQVQTTILPTIGQFGGDEFERAKAIYDYLKSKGATNQAIAAILGN
                     WSVESSINPKRAEGDYLSPPVGATDSSWDDEGWLSLNGPTIYNGRYPNILKRGLGLGQ
                     WTDTADGSRRHTLLLEYAKGKHQKWYDLELQLDFMLHGDSPYYTNWLKDFFKNTGSPA
                     SLAQLFLIYWEGNSGDKLLERQTRASEWYYQIEKGFSQPNGGTAQSDPKALEAVRGDL
                     FENSIPGGGDGMGYAFGQCTWGVAARINQLGLKLKGKNGEKIPIISTMGNGQDWVRTA
                     ASLGGEIGTSPQEGAILSFAGGGHGTPTEYGHVAFVEKVYPDGSFLISETNYNGNPNY
                     TFRKLSGVDSSLSFAYTTK"
     gene            complement(35285..36130)
                     /locus_tag="ACHU66_000151"
     CDS             complement(35285..36130)
                     /locus_tag="ACHU66_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFR3388468.1"
                     /translation="MNKAKLTALCHKISKNTGLTFNSVMTYYFLEVILKKLSQSSYSN
                     HYIFKGGFLLSNVIGVESRSTVDIDFLFHQITLSEETVKQQLKEILADSEEGISFVIQ
                     SITAIKESDDYGGYRATISCQLENIKQVIHLDIATGDVVTPQPITYDYKAIFDEDNFP
                     IIAYTIETILAEKLQTIYSRNFLNSRSKDFYDVYILSKLKKEDIDFIQLRNACQRTFS
                     YRETELDFVKIIELLERFKSDPIQNKQWQNYSKKYSYTKGISLADVLDEMIRLITVLI
                     SINSD"
     gene            complement(36127..36717)
                     /locus_tag="ACHU66_000152"
     CDS             complement(36127..36717)
                     /locus_tag="ACHU66_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFR3388469.1"
                     /translation="MSKKEILLDFIEKNNGIVTNKDCKALGIPTIYLTRLEKEGIIFR
                     VEKGIFLTQNGDYDEYYFFQYRFPKAIFSYISALYLQQFTDEIPQYFDVTVPRGYRFN
                     TPPANLNIHFVSKEYSELGMTTVPTPLGNNVRVYDFERIICDFVIHREKIDSELFVKT
                     LQSYGNYPKKNLAKLYEYATKMNTLEKVKQTLEVLI"
     gene            36996..41891
                     /locus_tag="ACHU66_000153"
     CDS             36996..41891
                     /locus_tag="ACHU66_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0018262 - isopeptide cross-linking
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SspB-related isopeptide-forming adhesin"
                     /protein_id="HFR3388470.1"
                     /translation="MTKTCNHHFLVNQEKGEKHVFRKSKKYRTLCSVALGTMVTAVVA
                     WGGTVAHADEVTSSVDTNIQRTENPATNLPEAQPKPVSEQTEILALTGQSNGAIAVTV
                     PHDTVTQAVEEAKAEGVSTVEDSQMDLGNTTSAAETNQQISKAEADAQNQVEAINEVT
                     ETYKADKAAYVDEKARIEQENKELSQAYEGANQTGKETNTWVDTKVKDLKTRYADADV
                     TVKEQVVSSGNGTSVLDYTNYGKAVETIQSTNEQAVADYLTKKTKADDIVAKNQAIQK
                     ENEAGLAKAKADNEAIERRNQAGQAAVDAENRAGQAAVDQANQEKQQLVSDRAAEIEA
                     ITKRNQEKEAAARKENEVIDAYNTKEMERYQRDLAEISKGEEGYISEALAQALNLNNG
                     EPQAQHGAITRNPNQIISTGDAMLGGYSRILDSTGFFVYDSFKTGETLSFNYQNLQNA
                     RFDGKKISRVTYDITNLVSPAGTDAVKLVVPNDPTEGFIAYRNDGNGDWRTDKMEFRV
                     VAKYFLEDGTQVTFSKEKPGVFTHSSLNHNDIGLEYVKDTSGKFVAINGSTVQVTNEG
                     LAHSLGFNRASDLNLPEEWDTTSSRYAYKGAIVSTVTSGNTYTVTFGQGDMPQNVGLS
                     YWFALNTLPVARTVTPYSPKPHVAVELEPVPEPITVTPDVFTPKTFTPEKPVTFTPKP
                     LEEVVQPSLTLTKVNLPVKPIPKELPTPPQVPTVHYHAYRLTTTPEIMKEVVNSDQAN
                     LHEKTVAKDSTVIYPLTVDALSPNRAQTTSLIFEDYLPAGYLFDKETTQKENGNYVLS
                     FDATKNFVTLTAKENLLQEVNKDLTKVYQLNAPKLYGSVQNDGATYSNSYKLLLNKGT
                     TNAYTVTSNVVTVRTPGDGETTTLITPDKNNENADSVLINDTVVALGTTNHYRLTWDL
                     DQYKGDRSAKETIARGFFFVDDYPEEVLDVVENGTAVTTLDGQKVSGITVKNYASLNE
                     APKDLQDKLARAKITPTGAFQVFLPDDNQAFYDQYVQTGTSLALLTKMTVKDSLYGQT
                     KTYTNKSYQVDFGNGYETKEVTNTLVSPEPKKQNLNKDKVDINGKPMLVGSQNYYTLS
                     WDLDQYRGIKADNSQIAQGFYFVDDCPEEALLPDEAAIQFITSDGKTVSGITVKAYSQ
                     LSEAPKMLQAALSKQKIQPKGAFQVFMPEDPQAFFESYVTKGENITIVTPMTVLETML
                     NSGKSYENVAYQVDFGQAYETNTVTNFVPKVTPHKSNTNQEGISIDGKTVLPNTVNYY
                     KIVLDYSQYKDMVVTDDVLAKGFYMVDDYPEEALTLNPDGIQVLDKDGNRVSGISVST
                     YASLSEASNVVQDAMAKRQFTTKGAIQVLSSDDPKTFYETYVKTGQTLVVTLPMTVKN
                     ELTKTGGQYENTAYQIDFGLAYVTETVVNNVPKLDPQKDVVIDLSHKDDSLDGKEVAM
                     NQTFNYRLVGAMIPSNRATDLFEYGFEDNYDEKHDEYNGVYRSYLMTDVILKDGSVLK
                     EGTEVTKYSLQQVDTENGLVSISFDKSFLETISDDSAFQADVYLQMKRIATGQVENTY
                     LHTVNGYVISSNTVVTHTPQPEEPSPNQPTPPQPPIETIEPPVPASILPNTGEQESLL
                     GLIGAGILLGTAYGLKKKEEK"
     gene            41892..42083
                     /locus_tag="ACHU66_000154"
     CDS             41892..42083
                     /locus_tag="ACHU66_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFR3388471.1"
                     /translation="MNPKTIYEKDSDQDGLTDAQELALGTNPQSVDTDGDGQADLEEL
                     QSGHSPLVPQKELCDGLEL"
     gene            42067..42618
                     /locus_tag="ACHU66_000155"
     CDS             42067..42618
                     /locus_tag="ACHU66_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388472.1"
                     /translation="MAWNFDTMKEALSEMEKVNYQEFIKAFLSLELSISDRTILNQVY
                     QDYMDEDDLSLISDELRVKVEGYQDEVKADMTDILEKLYRTGEGSSFIMDLMSSNSLS
                     DTLEQYEVLDSDDYSPLSLETLQAMIQQDLAISSQDYFGDLVHLALQKDLLDQKSHFL
                     QYYVATVMEGIPQERDQRALVLD"
     gene            42668..48631
                     /locus_tag="ACHU66_000156"
     CDS             42668..48631
                     /locus_tag="ACHU66_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFR3388473.1"
                     /translation="MNQEVLLQMMRATIPRDRALLEAFLYYQAEHFDEEWDSLVYQFM
                     TNRQEINKSVQVLHFETDVSAFVQASPYDTAHDLLTYTQVFGQTGLQKLDKLSPSEKD
                     LVIEVALFNLATRFQLLDSNGHYQTISPDSLLQKSRGANLVNVYRVANNLAERISRDI
                     EQFLLTYEPELETRVEETVQEKEETVDEHKTSVHQAISFREEGSLVIASLDVDLSQLD
                     IRTGKTSHLPAYEELSLRRKFEILTYFDQIRNERSKLPNLRRGDFDTEMEKTPIFDGE
                     ELLTYLEADGSLYELKRTLTTVEEKELEKIGQAIRTENQEKLTQVGIDLSQFDSDQIG
                     ILLDAAGRFHLKNADLALLGGYPNASVTQLALATELLQMGLSHEKIEFFFGSQLSIEE
                     LRQVAYAFLHQELSIEDAEQFEKDKGNQPDLTLRDWKNKLEKAEGKEVVDEAFEVNPL
                     VQMVLDTFPLGSLVSYKGQDFEVVSVSDARLNGLIRIELVNDFSDIIEQNPVLYLRTW
                     EEVSQALHQPKAEPQTELDDEDQELNLFSFLEEETSQSVNEAVQNIGVLDPEQSGEAS
                     HDSEKPTIYVPEEVLVESIPDIPATAFYFPEDMTDFYPKTARDKVETNIAAIRLVKTL
                     ETERRQASTSEQELLAKYVGWGGLANDFFDDYSPKFSKEREELKSLVTDKEYSDMKQS
                     SLTAYYTDPALIRQMWDKLERDGFTGGKILDPSMGTGNFFAAMPKHLREKSELYGVEL
                     DTITGAIAKHLHPNSHIEIKGFETVAFNDNSFDLVISNVPFANIRIADNRYDKPYMIH
                     DYFVKKSLDLVHDGGQVAIISSTGTMDKRTENILQDTRETTEFLGGVRLPDSAFKAIA
                     GTSVTTDMLFFQKHLDKGYLADDLAFSGSIRYEKDSRIWLNPYFDGEYNSQVLGTYEV
                     RNFNGGTLSVKGTSDDLIASVQTALNHVKAPREIDRNEVIINPNVLTKQVIDTSIPAE
                     MRENLGQYSFGYQGSTVYYRDNKGIRVGTKMEEISYYVDEEGNFKAWDTKHSQKQIDR
                     FNNLDVTDSTALDVYVTDDAAKRGQFKGYYKKTVFYEAPLSDKEVARIKGMVDIRNAY
                     QEVIAIQRHYDYDKKTFNHLLGKLNRIYDSFVKRYGYLNSTVNRNLFDSDDKYSLLAS
                     LEDESLDPSGKSVIYTKSLAFEKALVRPEEEVKKVHTALDALNSSLADGRGVDFAYMM
                     SIYQVESKMTLIEELGDLIMPDPEKYLNGELTYVSRQDFLSGDVVTKSEVVDLFVKQD
                     NQDFNWSHYAGLLEAIKPARITLADIDYRIGSRWIPLSVYGKFAQETFMGKSYELSDQ
                     EVATVLEVSPIDGVITYQSKFAYTYSNATDRSLGVPASRYDSGRKIFENLLNSNQPTI
                     TKQVVEGDKKKNVTDVEKTTVLRAKETHLQQLFQDFVSKYPEVQQMIEDTYNRRYNRT
                     VSKVYDGSHLTIDGLAQNISLRPHQKNAIQRIVEEKRALLAHEVGSGKTLTMLGAGFK
                     LKELGMVHKPLYVVPSSLTAQFGQEIMKFFPTKKVYVTTKKDFAKAKRKQFVSRIITG
                     DYDAIVIGDSQFEKIPMSREKQVTYINDKLEQLREIKLGSDSDYTVKEAERSIKGLEH
                     QLEELQKLERDTFIEFENLGIDFLFVDEAHHFKNIRPITGLGNVAGITNTTSKKNVDM
                     EMKVRQVQAEHGDRNVVFATGTPVSNSISELFTMMNYIQPDVLERYQVSNFDSWVGAF
                     GNIENSMELAPTGDKYQPKKRFKKFVNLPELMRIYKETADIQTSDMLDLPVPEAKIIA
                     VESELTQAQKYYLEELVERSDAIKSGSVDPSRDNMLKITGEARKLAIDMRLIDPVYSL
                     SDNQKILQVVDNVERIYREGAGDKATQMIFSDIGTPKSKEEGFDVYNELKVLLVDRGI
                     PKEEIAFVHDANTDEKKNSLSRKVNSGEVRILMASTEKGGTGLNVQSRMKAVHHLDVP
                     WRPSDVGRILRTFKIKKNVEVTDNGKDNF"
     gene            48612..48986
                     /locus_tag="ACHU66_000157"
     CDS             48612..48986
                     /locus_tag="ACHU66_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HFR3388474.1"
                     /translation="MAKTIFEEMGGKYERQGDYLIPCLTVPAEEEQPIGIWGQRHLDY
                     LKHHCKVTYTNLLTSGRLNAYLADIDRQAQERFERLIEGMKQAQGITEQLKAENALEW
                     TGCLNNIRACAREIVEKEIIFA"
     gene            49353..51272
                     /gene="tet(O)"
                     /locus_tag="ACHU66_000158"
     CDS             49353..51272
                     /gene="tet(O)"
                     /locus_tag="ACHU66_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(O)"
                     /protein_id="HFR3388475.1"
                     /translation="MKIINLGILAHVDAGKTTLTESLLYTSGAIAEPGSVDKGTTRTD
                     TMNLERQRGITIQTAVTSFQWEDVKVNIIDTPGHMDFLAEVYRSLSVLDGAVLLVSAK
                     DGIQAQTRILFHALQTMKIPTIFFINKIDQEGIDLPMVYQEMKAKLSSEIIVKQKVGQ
                     HPHINVTDNDDMEQWDAVIMGNDELLEKYMSGKPFKMSELEQEENRRFQNGTLFPVYH
                     GSAKNNLGIRQLIEVIASKFYSSTPEGQSELCGQVFKIEYSEKRRRFVYVRIYSGTLH
                     LRDVIKISEKEKIKITEMCVPTNGELYSSDTACSGDIVILPNDVLQLNSILGNEMLLP
                     QRKFIENPLPMLQTTIAVKKSEQREILLGALTEISDGDPLLKYYVDTTTHEIILSFLG
                     NVQMEVICAILEEKYHVEAEIKEPTVIYMERPLRKAEYTIHIEVPPNPFWASVGLSIE
                     PLPIGSGVQYESRVSLGYLNQSFQNAVMEGVLYGCEQGLYGWKVTDCKICFEYGLYYS
                     PVSTPADFRLLSPIVLEQALKKAGTELLEPYLHFEIYAPQEYLSRAYHDAPRYCADIV
                     STQVKNDEVILKGEIPARCIQEYRNDLTYFTNGQGVCLTELKGYQPAIGKFICQPRRP
                     NSRIDKVRHMFHKLA"
     gene            51324..51497
                     /locus_tag="ACHU66_000159"
     CDS             51324..51497
                     /locus_tag="ACHU66_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002779755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFR3388476.1"
                     /translation="MMKCEWILCPVCGSKTRNKIRKDTVLENYPLYCPKCRQESLIKV
                     DNLKITVIKEPDA"
     gene            51987..52496
                     /locus_tag="ACHU66_000160"
     CDS             51987..52496
                     /locus_tag="ACHU66_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFR3388477.1"
                     /translation="MKYAPRKVYIKESGGYVELSYTDFCRRRQADKGYMDKLFIPVQG
                     CLLEVVREQYADFYRDRERWRYLQKLDARNSLLSLDGFTDSEGNPLDFIADEAADIAE
                     TVVNAVMVDRLKAALPLLSDSEQELIQAIFFDGLSEREVGARLGITQSVVNKRKARIL
                     RKLRKIIEN"
     gene            52591..53016
                     /locus_tag="ACHU66_000161"
     CDS             52591..53016
                     /locus_tag="ACHU66_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFR3388478.1"
                     /translation="MAYNHGREDRKWRIWKEAEEKLLRECGVDEVTIEQIRIADRADF
                     NSNRRFYRWTNDVAEYLEDMADRERQAEVNTVAELLDEIESENLYQVLVTVDGRTLKI
                     VLLKMQGYSTKEIAPLVHLTTGAIYARLDHLRKKLRKIL"
     gene            53100..53573
                     /locus_tag="ACHU66_000162"
     CDS             53100..53573
                     /locus_tag="ACHU66_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFR3388479.1"
                     /translation="MAYKAKAYTLREESTESGIRYFISFKDGQGEHHELEVSEQLFFE
                     FRQMERRNRNLLQWDERHREFSEVWDETLNRRALKLPKSIEEQMIEAERAELLCKAVD
                     RLPEIQRRRFLLYYEYEFNFYQIAAMEHCTASAIQKSVAVAKKKVKAEMRKYLQP"
     gene            53727..54758
                     /locus_tag="ACHU66_000163"
     CDS             53727..54758
                     /locus_tag="ACHU66_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR3388480.1"
                     /translation="MQKLQTVNAETLLYEPLEKPSFVVDSLIPTGLSLFCGSQKIGKS
                     WLMLKLCLCVSQGIPLWDMTTMEGDVLYLCLEDTFCRIQDRLFRLTDEASGRLHFAVA
                     SCKLSDGLIVQLEDYLKDYPDSRLIVIDTLQKVRTASKDNAYASDYGDISLIKDFADR
                     HSLAVIVVHHIRKQNDSDVFNKVSGTTGLTGSADATFVLEKEKRASDTAKLYVTGRDT
                     PYQEYTLRFRDCSWELVERKTQEQLAKETIPDVLFRLVDFMRDKEEWAGTATELLAAM
                     GETETIPTVITKWLNEYHTTFLSENRICYQYSRRKDGRQIALARRAGDSGDGGDSDIG
                     IPPVTVIDA"
     gene            55004..55270
                     /locus_tag="ACHU66_000164"
     CDS             55004..55270
                     /locus_tag="ACHU66_000164"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388481.1"
                     /translation="MPDVPSGLPYGRSYALLSYALRLRIKERRHDGYSSCEGVSQKHR
                     HHRHDRHAGDKGDSYGNRQGASQNCRHHRHRCHPKGSYPPKKGR"
     gene            55277..56242
                     /gene="mobV"
                     /locus_tag="ACHU66_000165"
     CDS             55277..56242
                     /gene="mobV"
                     /locus_tag="ACHU66_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFR3388482.1"
                     /translation="MPYAILRFQKRKAGGVAACERHNERKKEAYKSNPDIDMERSKNN
                     YHLVAPPKYTYKKEINRKVAEAGCRTRKDSVMMVETLITASPEFMNQLPPEEQKAYFT
                     MALDFISERVGEQNILSAVVHMDERTPHMHLCFVPITPDNKLSAKTILGNQKSLSEWQ
                     TAYHERMSSRWNQLERGQSSMETKRKHVPTWLYKLGGRLDKQYGEIVSALSDINAFNA
                     GKKRDKALELVAAWLPEVEKFSKEIGRQQAYIDSLKEQIGQEADYAGRMRDEKYEQEL
                     KVQKANQRIFELQRTNEQMGRLLSKIPPEVLEELQRTGRNKSRER"
     gene            56250..57104
                     /locus_tag="ACHU66_000166"
     CDS             56250..57104
                     /locus_tag="ACHU66_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR3388483.1"
                     /translation="MKKQDFKVLKTKDLYPFPDNPFHVVEDETLSELAESIKEFGIVT
                     PIITRPKEDGNGYEVIAGQRRVRASELAGINTVPAFVLPLDRDRAIITLVDSNLQREN
                     ILPSERAFAYRMKSEAMKRQGFRTDLTSSQVVTKLRTDDKVAQGFGVGRMTVQRFIRL
                     TELIPPILQMVDEGKIALTPAVELSFLKKDEQENLFATMESEEATPSLSQAQRMKSMS
                     QSGQLDMDMIFSIMTEEKGNQKETLKINTSKLKKYFPKDTTPKQMEETIIRLLERELQ
                     RKRSRDSR"
     gene            57181..57411
                     /locus_tag="ACHU66_000167"
     CDS             57181..57411
                     /locus_tag="ACHU66_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PC4/YdbC family ssDNA-binding protein"
                     /protein_id="HFR3388484.1"
                     /translation="MKEIQYEIVKEIAVLSASDSGYTKEINLISWNGREPKYDIRSFS
                     PNREKCGKGITLNADEAAALLEALQKEVNSGD"
     gene            57482..57673
                     /locus_tag="ACHU66_000168"
     CDS             57482..57673
                     /locus_tag="ACHU66_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HFR3388485.1"
                     /translation="MGEDRKTTGRRKRIVPKGQVQMIISREYIGTQTVAEAFVPIISE
                     DIRKKIAEGDTFDNEGLSA"
     gene            57727..59610
                     /locus_tag="ACHU66_000169"
     CDS             57727..59610
                     /locus_tag="ACHU66_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR3388486.1"
                     /translation="MAGIRTENKIYEVGIYCRLSKDDGTDNESASIATQKSILTDYVK
                     RQGWHLAKTYVDDGYSGTNFQRPSFQNMIKDIENGLINCVITKDLSRLGRNYLDCGLY
                     LEVFFPEHNVRYIAVNDGVDTLNKSAMDITPFRNILNEMYSADVSVKIKSAYRARFQQ
                     GKFMGTTAPYGYVKDPADHNHLLIDDKVAHVVREIFDLALAGNGIAKIRKHINKQHIL
                     RPAAYAVEQGATGYERYFEDNEENRYIWSENSVRGILRSPIYAGNLAGYKRIAANMKS
                     KKRPSKLPEEWEVIPDTHEGIVTQEEFDTVQQLMTSRRREQNAGGFENIFSGVIKCAD
                     CGYAMRAASANRRKRPDIIDCVQYTCNNYGRYGNVMCTAHSIEARDLFNAVLADINRF
                     ADMAVNDEKAVRAIERRLTETDQSRAKSLEKEKKKLNKRLAELDRLFSSLYEDKVMER
                     ITERNFEMMSGKYQKEQLEIEARLKEVTETLNDSYEKSQGVRDFLSLIRNYQGLKELD
                     ATIINALIDKILVSEREKLADGTVRQEIKIYYKFIGFVGELHITPTKRWTALKPKNCT
                     VCGVEYVPSSGISKYCPACAKRIQREKSNESKRRSRERNRRACIELSAKNDRLILSSE
                     MDD"
     gene            59577..60536
                     /locus_tag="ACHU66_000170"
                     /pseudo
     CDS             59577..60536
                     /locus_tag="ACHU66_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071192.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            60607..60906
                     /locus_tag="ACHU66_000171"
     CDS             60607..60906
                     /locus_tag="ACHU66_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5962 family protein"
                     /protein_id="HFR3388487.1"
                     /translation="MTKTVEEMRYQLEEWLSQGFTSSEDRANYQTLKEQYEDETFDYS
                     FSRREITGQLELIITSRENDFPNLDEVTKAEYLDLVAQLDDLDKRQADYYRKQLA"
     gene            60920..61210
                     /locus_tag="ACHU66_000172"
     CDS             60920..61210
                     /locus_tag="ACHU66_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5966 family protein"
                     /protein_id="HFR3388488.1"
                     /translation="MLEQILQSLLIIAAIGLMLFVLYRIVKVSGALFLIGLISGLVFI
                     EIYGIYLFFTERHLYSEDLAANGIWSFTGFYIALNLFLAISLSMRWWRNRIV"
     gene            61305..61943
                     /locus_tag="ACHU66_000173"
     CDS             61305..61943
                     /locus_tag="ACHU66_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR3388489.1"
                     /translation="MLNKVKARFLIGVGGLIAVSFMVMIGYTIGSQTISKQTENQIRA
                     EANKLVSKKNQEERATVLSDELVKEFLTQYYTKVQLGENNSRIKTYMTDSAFSEEEAN
                     QNKAINQVYKDYMLDYRFESASIYVNTESNVALAEVSYQVTYVSDLSEQQQRTTQTET
                     KTVILSYSKVSDKLLVNQLTIWNGKLEDMKEATNGANSSIPTIQGTTTSENN"
     gene            61983..63083
                     /locus_tag="ACHU66_000174"
     CDS             61983..63083
                     /locus_tag="ACHU66_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFR3388490.1"
                     /translation="MTRIKAVKQKAILDVAESLGCSFRRLSGHIYEHPDHDSFRIFAD
                     TNTFKWFSRDIQGDVIDFVQLVTGVSFKEAVSYLETGDFEQVKLIEETYQPFQYYLHE
                     EPFQQARTYLQVVRGLSDQTINTFGRQGLLAHATYQSEPVLVLKSYDHNGTLQAASLQ
                     GLVKNEEKHDRGYLKKIMKGSHGYVGISFDIGNPKRLIFCESVIDMMSYYQLYQKQLS
                     DVRLISMEGLKLSVIAYQTLRLAAEEQGKLAFLDTVNPSRLSHYLQAIQETTTFFQTH
                     SNALTLAVDNDEAGREFCQKLSDKGLPLSQDLPPLQGLETKLDWNDIVKRQKELSLRD
                     LIQSAQTKVIRDHPPPKREHTFDVITKSSPLS"
     gene            63111..63338
                     /locus_tag="ACHU66_000175"
     CDS             63111..63338
                     /locus_tag="ACHU66_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5965 family protein"
                     /protein_id="HFR3388491.1"
                     /translation="MSVIERLAEKVARQEEKVSRETEKLEHYRDQLQTAMYSTFIKRQ
                     QSSQLSFHEALEQAFGKETTLHPNYRNEDTE"
     gene            63335..63724
                     /locus_tag="ACHU66_000176"
     CDS             63335..63724
                     /locus_tag="ACHU66_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5945 family protein"
                     /protein_id="HFR3388492.1"
                     /translation="MSKTWNFDQPLDDVKPTSSHEERAKIAALFHKQDEKPIEEVDYV
                     AAFEQQQKESESKDVQTPESKVKQSQPKKVNITSDYKQHLADTIAQNNKDISACQKQI
                     EELHQLIDEKKIQNKKLQAISVAIDDL"
     gene            63775..64065
                     /locus_tag="ACHU66_000177"
     CDS             63775..64065
                     /locus_tag="ACHU66_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388493.1"
                     /translation="MKFFQKKKQNQDQFKRLIHRLSEMSDSELTKVEQLLDVVFDSSF
                     KSSERVEISRDIITDEEQILDKSIQEAKNKLNTEQLEKRIEEFKQSKKPKNG"
     gene            64135..64611
                     /gene="pezA"
                     /locus_tag="ACHU66_000178"
     CDS             64135..64611
                     /gene="pezA"
                     /locus_tag="ACHU66_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PezA"
                     /protein_id="HFR3388494.1"
                     /translation="MIGDNIKSLRRTHDLTQPEFAKMVGISRNSLSRYENGTSTVSTE
                     LIDRICQKFNVSYVDIVGEDKMLTPVEDYHLTLKVEVIKERGAAILSKLYRYQDSQDI
                     VFDDESNPWILMSDDLAELINTKIYLVATFDEIERYNGYLDGIERMLDMVHHRVVA"
     gene            64611..65378
                     /gene="pezT"
                     /locus_tag="ACHU66_000179"
     CDS             64611..65378
                     /gene="pezT"
                     /locus_tag="ACHU66_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin PezT"
                     /protein_id="HFR3388495.1"
                     /translation="MRLEEFSEAEFQKALQRTIRALTRGKTIPDQPKAILLGGQSGAG
                     KTTIHRIKQKEFQGNIIIIDGDSYRSQHPNYLALQEKYGKDSVDYTKGFAGKMVEQLV
                     DELSTQGYHLLIEGTLRTTQVPRQTAQLLSSKGYQVSLAVIGTKPELSYLSTLIRYEE
                     LYAIDPTQARATPKEHHDGIVENLVDNLRELESENLFDQIQIYQRDRTCIYDSETAEG
                     SAAEVLQECLFGKWSKVEEEMVKESELLLKELLEKNK"
BASE COUNT        20260 a        12452 c        14975 g        17697 t
ORIGIN      
        1 ggaacgtgat aaatagggca aatactgcct tcttttcgtg taaccttgct agccagcaag
       61 gtttttttct ctatatcgac tttcttgttt tctgtgtcat tgttatatag aataggagaa
      121 aagatgattt taaaagaaca gatgttactg gtggaccaag tccaactagc gccacgaatt
      181 tttgctatga ccttgcaggg tgatatggtc caagatatga aaatggggca atttatccat
      241 attcgtgtgc cggatgattc catgctcttg cggagaccga tttctatttc cgagattgat
      301 cggaataagt ctcaatgtcg gattatttac cgagtggaag gtcaagggac ggaagtcttt
      361 tcgaaaatga ctgttgggca gtatttggat gtcatgggac cgcttgggaa agggtttgaa
      421 gtagactttc tcaaagaagg tgaccgcatt ctcatcattg gcggaggaat cggtgttcct
      481 cccttggtgg aagtagccaa acgggctgcg ttccgtggtg tcaatgtcac gtcagtcatt
      541 ggctttgcca ccaaagaggc cgtcattttg gaagaggagc tggcaagtta tgggatggtc
      601 tatgtcacaa cggatgatgg ttcctatggg cgtaagggga atgtcgcaac ggtcgttgaa
      661 gagctgacaa acgagtttgc agccatttac tcatgtgggg cgccagctat gatgaactat
      721 gttgatcaac gctttcagga acatcctcat gcctacattt cactggaggc taggatggct
      781 tgcgggatgg gggcgtgcta tgcctgtgtt gtcaaaccca aagagggaca agaacacgag
      841 aacaagcgtg tctgtaagga aggtccagtc tttgcgactg gtagtctgat tttataggag
      901 gttgatatga taaaacgatt agcgatttcc ttaccaggct tggacttgaa aaatcccatc
      961 attccagcct ctggctgctt cggttttggt caggagtatg cagattatta tgaccttaac
     1021 cagcttggat ccattatgat taaggcgact actcgtcatc ctcgttatgg caatgcgact
     1081 ccccgtgtag ccgagacacc agcaggtatg ctcaatgcca ttggacttca aaatccgggt
     1141 gttgatgtag tcttatccga aaaattacca tggttggaac agcattttcc agacttgcct
     1201 atcattgcca atgtggctgg tttttccaat gaagaatatg cctatgtgtc agggaaaatt
     1261 tcaaaggctc caaacgtaaa agctatcgag ctgaacattt cctgtcccaa tgtggaccac
     1321 ggaaataatg gtctcttgat tggacaggtg ccagaattag cctatcaagc agtaaaagca
     1381 gcagtcgaag cctcatctgt acctgtttac gttaagctga caccaagtgt ggcagacata
     1441 accctgttgg ccaaggctgc tgaggatgct ggagcgactg gcttgacgat gataaatacc
     1501 ttggttggta tgcgattcaa tctcaaaact cgacaaccaa tcttggcaaa tggtacagga
     1561 ggaatgtcag gtccagcagt ttttccggtt gctctcaaac tcattcgtca ggttgctcag
     1621 gtgactgatt taccgattat tggtatgggt ggtgtggata cggcggacaa ggctatagag
     1681 atgatggttg caggagcttc agctgtaggg gtaggaacag ccaactttac agacccattt
     1741 gcttgtccaa agattattca agacttgcca aaacgcatgg aagtttatgg tattgatagt
     1801 ttggaaggtt tacgccgaga aatcagacat agattgggta agcgttgaca aaggtaaaat
     1861 aaaagagtaa tataaagaaa atatctcctt taactaagtc cagagaggct aggaaggtgt
     1921 catgtaatat gcttgaaagg tgattttcaa gctgaattcg tgcacatccc tgaccgattt
     1981 ggcagggatt tttgtgtaaa tgaaaggatt ttatgaaaga aacaagacca attattgccc
     2041 ttgactttgc aggtaaagaa gaagttcagt cttttctgga acagttccca ccagatgaga
     2101 aactctatgt aaaggttgga atggagttgt attatgcgga gggaccaggt attatcaatt
     2161 atttgaaagg ctgcggtcac agtatttttc tagatttgaa actgcacgat attcctaata
     2221 ccgtcgagtc tgctatgaaa gtcttggcaa aactcggtgt ggatatgacc aacgttcacg
     2281 ctgctggtgg tgttgagatg atgcgggcag cacgaagggg attgggtaag gatgctgttt
     2341 taatagcggt cactcagttg acctctacct cagaggagca gatgagaaca gaccagaata
     2401 tacagactag tttgcaggaa gcagttgttc actatgctca aagagcacaa gaagctggtc
     2461 ttgatggggt agtttgttca gcgcatgagg tggctttgat taaagatgct acaagttctg
     2521 attttgtgtg cctgacgcca gggattcgtc cgactggtgg tgaagtaggt gatcagaaac
     2581 gagttatgac accagcagat gctgctcgaa tcggtagtga ttacattgtg gtaggtcgcc
     2641 cgattacaaa atcagaacat ccttaccaga cctatttgtc tattaaagag gagtggaatc
     2701 gtggataaga tttatcaggt tataatcgat agacctatcg ggtatattga taaatttggc
     2761 aaccagtacc ccatcaatta cggctatgtt ccaaatctat tggctggaga tggtgaagag
     2821 caagatgttt acatcatttc caaagctgtt cggcagccct tgacagcttt cgaggggaaa
     2881 ttggtcgcga ttatacatcg cagagatgat gttgaggata agtgggttct gacaggcctt
     2941 gatgaaaaac ttaccgtgac agaaataaaa gagaaaacca atttcattga acagtatttt
     3001 gattcatgga ttgaaatggt ggactaagag aagtctgtaa gaattcaacc tggataaata
     3061 tagtttttat agggttcata gattgaacca atgctttagg agtgacaggc tattcggcgg
     3121 gatggtcatg ttggaatggt acgcatttag aaggaggtct attatgacat tagcaacaga
     3181 aatcgcatca cacttattgg atattaaggc agttcatctg cgtccagaaa agccatttac
     3241 atgggcatca gggatcaaat cacctatcta tacagataat cgtattacac tatcttatcc
     3301 tgaaactcga aatttgattg aagatggctt tgttcagcgt atcgaagaag aatttcctga
     3361 ggttgaggtg attgccggaa ctgccacagc aggtattcct catggtgcta tcattgcaga
     3421 taggatgaat ttgccatttg cttatatccg tagcaaacca aaagatcatg gtgcagggaa
     3481 tcaattagag ggtcgcatcg tcaagggaca aaaaatggtt gtcgttgaag acctgatttc
     3541 tactggaggg tctgttctag atgccgtagc agcagctgag cgtgaaggcg ctgacgttat
     3601 tggtgttgtg gcaatcttta cctatgaatt gccaaaggca gattgtaatt ttgataatgc
     3661 tggcgtgaaa ttggtgacgc ttagcaatta ttcagagtta atcaaggtcg caaaagtaca
     3721 gggatacatc aatgcagatg gtttgaatct gcttaaaaag ttccgtcaaa atcaagaaac
     3781 ttggcaggaa gactagattt aaaacgagcc cacgcacgat ttctgggaac ttatcctgta
     3841 aaatggtata atagtctcta acgtattttg agaatacaat aattgaccgt atgaggagaa
     3901 gaacatgatt tcagttatcg tgccttgttt taatgaagaa gaggcaattc cctattttta
     3961 cgatgctatg gagaaagttc gtaaggagat gggggaacaa tttgagtata tttttgtcaa
     4021 tgatggttca aaggatagga cattaacggt tttacgccaa ttgtctggtc aagaccgagc
     4081 tgttcgctat ctttcctttt caaggaattt cgggaaagaa gctgctctct atgcaggtct
     4141 acaagctgcg caaggtgagc tagtgactgt catggatgtt gatttacaag atccacctga
     4201 aatgttgatg gaaatgaagg ctatgttgga tggaaatcca gatttagatt gcgttggaac
     4261 ccgtcgtgtc agtcgagatg gtgagccacc gattcgtagc ttttttgcaa aattattcta
     4321 caaattgatg aataaaatca gccaggtcga ggtagtagac ggtgcccgtg atttccgtct
     4381 catgcgtcgt cacatggtag atgccattct atctgtgtct gaatacaatc gcttttcaaa
     4441 agtaattttt gcttgggtag gattcgaaac agaatacttg ccctatgaaa atgttgagag
     4501 ggtcgctggc gaaactagtt ggtctttctg gaaattgctt tcttattcta ttgaaggaat
     4561 catcaatttt tcagatacac ctttgaatat tgcctcctat acaggtttct tcaccttcct
     4621 attgtcgcta gttctaatgg tttttgtggt tttcaaaacc ttggtgttcg gagatcctac
     4681 aattggctgg ccgtcgacta tttgtatcat cctatttttg ggaggtctgc agttgatgac
     4741 cattggtatt ctcggtaaat atttggcaaa agttttcttg gaaacgaaga aacgtccgat
     4801 ttatattgtt aaagagaaaa gttcaaacta ggagcagttt atggaacatt cagcttggca
     4861 cgaattgata aaggcacagt tgccagagca ttattttggt aaaatcaatc agtttttaga
     4921 ttatgtttat gctggtggaa ccgtttatcc gcctcgtgag aaggtatttg ctgccattca
     4981 aacaacggat ttggaagagg ttaaagtagt tattttggga caggatccct atcacggacc
     5041 tggtcaggca cagggcttgt cattttctgt tccgaactcg gtgccagctc cgccatctct
     5101 tcaaaacatt ttaaaagaat tgacggatga tattggcgtc aaacaagatc atgatttgac
     5161 agcatgggcg gaacaaggtg tgcttttact caatgcttgt ttaactgttc cagcaggtca
     5221 ggcaaatgga catgcagggc aaatctggga gccttttacg gacgctgtta ttaaggtggt
     5281 caatagctta gaccagcctg ttgtctatat tctttggggt gcttatgctc gcaagaaaaa
     5341 agcgttgata accaacccaa aacatctggt tatcgaatct gctcatccaa gccctctttc
     5401 agcctatcga ggtttctttg gaggcaagcc cttctcacaa gcaaatgcct acttgacttc
     5461 acaaggcaga acaggaattg attggctgcg atagtagatg gtaatggctt tggtactaca
     5521 aaggagaata tgaatgttat tagtgaaaaa tggtcgtgta ttggatccgc aatctggttt
     5581 agacaaggtt tgcgacatct tgatggacgg aagtgagatt gttaagattg cggagtcgat
     5641 ttcagaagat gttgagcaag aagtgattga tgcgacagga ttagtcgttg ctcctggttt
     5701 ggtcgatgtt catgtgcatt ttcgggagcc aggtcagacc cataaagaag atattcatac
     5761 aggagctctt tctgcagcag cgggtggttt tacttctgtt gtcatgatgg ctaacactaa
     5821 tccgacgatt tcaaatgttg aaaccttaaa agaagtattg gattccgcta aaaaagaagc
     5881 tattcacatt tatagtgtgg caaccattac agatcatttc gacgggcaaa atctgaccga
     5941 ttttcctgct ttgctggaag ctggagcggt ggccttttcg gacgacggta ttcctttgac
     6001 aaatgctggc gtggttagaa aagctatgaa attggccaaa gagcagggct gtctcatcag
     6061 tctgcatgaa gaagaccctg acctgaatgg tgtcttggga ctcaacgagc aagtggctga
     6121 aaaacatttt catgtttgtg gagcaacagg cgtagcagaa tacagcatgg tcgctcgcga
     6181 tgtcatgatt gcctatgaaa caggtgcgcg tgttcatatc cagcatctat ccaaagccga
     6241 gtcggtcaag gtggttgaat ttgttcaagg tctcggagca aacgtcacgg cagaagtagc
     6301 accccaacat ttttcacgaa ctgaggactt acttctaata aaaggtgcca atgctaaaat
     6361 gaatccgcca ctacgattag aaagtgatcg cttggctgtg attgatgggt tgaaatctgg
     6421 tgttatctct gtcattgcga cggaccatgc acctcatcat gcagatgaga aaaatgtaga
     6481 ggaccttacg caagctccgt caggtatgac aggtttagaa acctcacttt cattaggtct
     6541 aactcatctg gtagaagatg gacacctcac tctcttgcaa ttgctggaga agatgactat
     6601 taaccctgcc aaactctacg gccttgatgc tggctatctt gctgaaaatg gcccagctga
     6661 tcttgttatt tttgacccaa atgcaaatcg gttggtcaca gcggattttg cctcgaagtc
     6721 tgctaattct ccgtttgttg gtgatagtct gaaaggacaa gtcctttaca ccattgcaaa
     6781 tggacaaata gtatttggta aataggaaat gagcggatta gatagctaat tcgcttattt
     6841 cgaacctttt tgacatgtaa gcgattttaa tgtttttgtt ttttgataaa aagtgtataa
     6901 tagagatata aaaaatgccg aagaaaggtc tatttatgaa aaagaaaaaa atcttgttgc
     6961 ctgtaatgtc cacactcttg ttggctccat ttgttttagc tcaacaagta caagctgctg
     7021 agacaacgac tgcagctacc acaaccaatc agccagcaac gacagatgca actgctacgg
     7081 taccagcaac atctgttgaa aatgtagcaa cggaggaaac tgttgtccct gctgcggaag
     7141 aaacagtaga ggcagtcatt atccacacaa acgacgttca cggtcgcatt cttgaagaaa
     7201 agaatgttat cggtgacgct aaggctgcgg cagtcattga agaagaacgt gccaaagtag
     7261 aaaatacaat tgtagtagat gctggtgatg ccttccaagg acttccaatt tccaactcta
     7321 caaaaggtga agaccgcgcc aatatcatga accaagtcgg ctatgatgca atggctgttg
     7381 gtaaccatga atttgacttt gggatggatc aagcaatcaa gtacaaggaa accttgaact
     7441 tcccgctatt gagtgctaac acttacgtta atggcgctcg tgtttttgaa gcttcaacta
     7501 ttgtcgataa aactccaact gttgtaggcg atgaatttgt agttatcggt gtaacaacac
     7561 ctgaaactgc tacaaaaacc catccgaaga atgttgaggg agtaacgttt accgacccag
     7621 tcacagaagt caataaagtg attgacgaag tggaagcacg tgctctagca gataatcgtg
     7681 tttacaagaa ctatatcatt ctagctcacc taggtgtcga ttctacaaca cctgttgaat
     7741 ggagaggttc aactcttgct gaagcacttt ctaaaaatag taaattagcc ggtaaacgtg
     7801 ttattgttat cgatggtcat tcccacacgg tagaagcgac tacctatgga gataacgtta
     7861 cttataatca aacaggctct tacttgaaca atattggtaa agtaaccttg aaatccgata
     7921 aattattggg tgaagctagc ttgatttctg ccgcagatac taagaacgtg acgccaaatg
     7981 ctaaaatcgc agccttggtt gatgaaatta aagcaaaata tgaagctgaa aatgctcaag
     8041 tagttattga aaataaccca gtggaattaa acggagaccg ctcaaacgtg cgtgttcgtg
     8101 aaaccaactt ggggaatgca gtgacggatg cgatttatgc ctatggtcaa acaggatttt
     8161 caaacaaaac tagccttgct gtgactaacg gtggtggtct acgtgcaaca attgctaaag
     8221 atcagcctgt tacaaaaggt gatattattg cagtcttgcc atttggtaac attgtttctc
     8281 aaatcactgt gactggtcag caaatttatg atatgtttac caagtcttta tcatctacat
     8341 tgcaagtgaa tccagaaact ggcgagatgc ttttagatga aaatggcatg ccgctctttg
     8401 aagctagtgg tggattccta cacatttcag gagcaaatgt attctacgat ccaactcttc
     8461 ctgtagaaga acgtgttctt cttatcggta ttcttaatcc agagactggc gaatatgacg
     8521 ccttggatct tgaaaaaacg tactatttag caacaaatga cttccttgct gctggtggtg
     8581 atggctacac aatgcttggc ggagcacgtg aagaaggtcc ttcaatggac agcgtctttg
     8641 ctgaatacct aaagacagct gacttatcag cttatgaggt tgtcaaccca tattcacgta
     8701 tcattcctgt aaattcaagc attgatacgg atgaagatgg ctacccagac tttatcgaaa
     8761 ttttattgga tactgatcca gaaaatccag cctcaaatcc agaaactgtt ccagccgaaa
     8821 atacggattc accatctaat caagtacaaa atacttcagc aacggacaag aaagctccag
     8881 ttgattctcc gaaagtagga gataaaaaaa cagaagtagc aagtccagta aaaactacaa
     8941 aagcaggagt cttgccaaat actggtgatc agatgaatct gacgctcagc ctgtttggtt
     9001 taggtcttgc aggactggcc atggcagtag gtcgtagaaa agaaaattaa tcaatcatct
     9061 gaaaacaggg tcttcggact ctgtttttga ctggaggaaa catgcaaact gtatttcaat
     9121 atcaaaaaga gggggatcgt gagcagatta gttcttacgt tctgcttagc aactccccac
     9181 gtcgtaaaca attattggga tttttacaac cacaaatcgc atcagttgaa gtggatgagc
     9241 gtggtatcga agaacatttt atgaaacaat tcgctacaga tgattttctc actcgagctg
     9301 caaaaacctg ttgtgaaatt tccaaagcca aatcggacat ggatttggta actggtcatc
     9361 tctacatttc tgcggataca atcgtcgtgt cagatgggca aatttttaac aaacctcaag
     9421 atttgacaga agctgaaatc atgttacgtt cctattttgg aaagagtcat cacgttgtaa
     9481 catctgtctg cctacggaca agacactcac tggatgtttt ttatacggtt gcacaaattg
     9541 attttgtcga ctattatcca gcccttgaag aggttattca atcatatatc cgtgaaaagc
     9601 agccactcga caaggcaggt gcatacggta ttcaggagct ggatccacgc tttgtgtcgg
     9661 gcatttcagg ggatattcac accattattg gtctacctgt agccgaagtt tcccgcagga
     9721 ttttttctga agaaggtttt gaaaaatagc agagctcaat agctagtttg tggtataata
     9781 gtggtcgaaa aggagagttt atgaccgtcg acattgaaag atacaagcaa ttggcccagc
     9841 agaagcaagg agaacatcga aaatttctag caaatttgaa gaaaaaagca cctaaaaatt
     9901 tggatagaat tgttcaagat attcatgccg aagtattttc tgaaatcgac tgtacagagt
     9961 gtgtcaactg ttgtaaaacg ttgggaccac tctttacaga agcagatatt acccgaattg
    10021 ccaagcattt acgcatgaaa cttccagttt ttgaggatat gtatttgcgt gtggatgagg
    10081 acggagataa ggtatttcaa tccatgccct gtcccttcct tggaggcgat aatctatgta
    10141 gtatctatga tgctcgtccc aaagcctgtc gagaatttcc gcataccgac cgcaagaaga
    10201 tttatcaaat caacaattta acaataaaaa atactctgat ttgtcccgca gcctatctct
    10261 ttgtcgaaaa gttacgggag agaatttaag aggaacaagt aagacaaaat attgattttc
    10321 tgctataata ataaaaaaac aagccgtttt tacggctttt ttcaatgaat catatttagg
    10381 aggaccccat ggcaacaatt caatggtttc cgggccacat gtcaaaagct cgcaggcagg
    10441 tgcaggagaa catcaagcac gttgatttcg tgaccatttt ggtcgatgcc cgtctgcccc
    10501 tatccagcca aaaccccatg ttgactaaga ttgtcggtga caagcccaag ctcatgattc
    10561 tcaacaaggt cgacttagca gaccctgttc gtaccaaaga atggcagagc tactttgaag
    10621 gtcaaggcat ccgtaccctt gccatcaact caaaagagca agcgacggtg aaaaaggtga
    10681 ccgacgcagc caagagtctg atgaaagaca agattgaacg cctccgcgag agaggtattc
    10741 aaaaagaaac cttgcggacc atgattatcg gtattccaaa cgctggaaaa tccactctta
    10801 tgaaccgtct ggctggaaag aaaattgccg ttgttggcaa caaaccaggt gtgacgaaag
    10861 gccaacagtg gctcaagtcc aataaggacc ttgaaatcct tgatacacca ggtattctct
    10921 ggcctaagtt tgaagaccag gaagtcggtc tcaagttggc tctgacagga gctatcaagg
    10981 accaactttt gcctatggac gaagtcacca tttttggttt ggactatttc aaggccaact
    11041 atccagaacg tctacaagag cggttcaagg gcatggattt ggagcaggaa acgcctgaac
    11101 tcatcatgga ctggaccaag aaattgggct ttcgtgatga ttatgaccgt ttttacaacc
    11161 tctttgtcaa ggacgttcgg gacggtcgct tgggtactta tacattagac agggtgagtg
    11221 acttagatgg caacgattaa ggaagtgact actctgctag ctcaggtgga cagtctggac
    11281 agtcctgtct gggcggaact atctcaggat gagcgagcag gggtccaggc tgccatcaag
    11341 aaacgccgta aggagctgga taaagaagta gcagaagatg cccgcttaga agctatgcta
    11401 ttttacgaaa aaaaccttta tgaaaatggg gtggagttca tcgctggtat cgacgaagtc
    11461 ggccgggggc ctctagcagg tcctgtggtg gcagcggcgg tcatcttgcc taaaggttgt
    11521 aaaatccgct acctcaacga ctccaagaaa atccctaagt ccaagcatga agcaatctac
    11581 caagaagtca tggagcgagc ggtggcagtc ggtgtgggaa taaaagatgc agctgtcatt
    11641 gaccaggtca atatctacga ggcgaccaag ttagctatgt tggaggcttt tgggaaatta
    11701 agccagaaac ctgaccactt gctcatagac gccatgaagt tggatacgcc cataccacaa
    11761 acttctatca tcaagggaga tgccaatagc ctatctatcg cagctgcctc tatcgtggcc
    11821 aaggtgacgc gggataagat gatggcggac tatgacaagg agttctcagg ctacggcttc
    11881 gccaagaatg ctggttatgg aacggcagag catttggaag gcttgaacaa gttaggcatc
    11941 acgcctattc accgtaagac ctttgagcct attaagtcta tggtaacagg agggaaatcg
    12001 tcgtgacggt ctttttctgg atttttctag cttccatagg cttatttggt ctcctatatt
    12061 tctgggagcg acggtcgatt tttatctcac tgtttgcctt gaatatcctc gtctgggcct
    12121 tggcatggat tatctcaaca ggcattctcg aaaacaatga aatcttgcgc ttgctggcta
    12181 tagcagtggc tatggtgctc tttttagcct tcttaagtgg tccatttgtc ttgcttttca
    12241 cactttatct aaatggtttt cagattttgg agagagaagg ggttcgcttc cataatttcc
    12301 tatccatggg actggcggta gccctgactt tctatctctt catcgctccc tttgtggttc
    12361 aatccctgtc tggtattagt ttgttaaaca tggtgtttgt ctatgttgga tttttagtgt
    12421 cttatgccat tatcatcagc atgctctata ccacgtctag ctttgtcaat tttgtcaatc
    12481 tcttcccagg aaagcttgat tatgtagtgg tcttaggtgc tggcttgatt ggtgacaagg
    12541 taacgccact tttggcttcg cggattgaca aaggcatagc gatttatcag aagcagcctg
    12601 gcagtaagct catcatgtca ggcggacaag gtcctgatga actcatagct gaaggtcaag
    12661 ctatggcaaa ttatgccttg gaacagggta tccctgcgga ggacatcatt attgaaaacc
    12721 aatcgaccaa cacagaagaa aatttgaaat tttcctatgc cctgatgaaa cctggcagtc
    12781 gatttgcact tgtaaccaat tactatcacg ttttcagagc tctgctattg gcacgacaat
    12841 tgaaaattaa atgtatcggc tacggcgcaa aaaccaaatt ttactttagc ctcaatgcct
    12901 ttattcggga atttgtaggc tatgtagtca tgagccgaaa gacgcatttg gtctttttga
    12961 gcatcgtgtc agccctttac ctgatcggag tgatcatcag tttaatgcat taaaaacagc
    13021 ctctaggtga ctagaagctg ttttcttata acataaagta gacaatggcg atatattgaa
    13081 gtgccgaagc tagtaggata aagagatgcc aaatcatgtg aaagtagggc tttttcttgg
    13141 cgtagaagag ggctccaata gtataagata atccaccagc cagcatgaga atgccaaatg
    13201 ccattccagt cttttgaaga atggctggca agataaagac aactagccag cccatgaaaa
    13261 gatagaggaa gaggctgaat ttttcgttta tttttttagc aaaaatttta tagagtatac
    13321 cgaaaatagt caatccccat tgcaaaccta caatcagata acctagccaa ccaccgacca
    13381 aggatagggc aacaggtgtg tagcttccag cgatagcaat gtaaatcatg ctatggtcga
    13441 taatcctcag gatgtatttg tgagtagaag catagggcat ggagtggtag atggaagagg
    13501 ataggaacat gagaaatagg ctgatgacaa agatggacat accaatcaca gcggtcaggt
    13561 tataatcttg ataggaatga atagcagtaa cggggagcaa gaccaacata agcagggctc
    13621 cgactgcatg tgtgacactg ttagcaacct cctctccaaa ggatagttgc aggctcaatt
    13681 tcaaactagg attcatggtg actcctttct aactccatag tgagttgacg ggttttgtag
    13741 taaagtttga cagattgaca agcgtagcga atgatgtcag gggtttcttc attttcctcg
    13801 tttcgcatgc agtcaacaaa atgattgtag agtgtttctg aatgactcga ttggagaaga
    13861 agatgtaagg tctgactgat ttcttgaata gaaaagacat tttttaagat agtcaaggca
    13921 atcaagcggg caacttgctg tttttgatat tttttcttaa caggtttttc aatctgttta
    13981 tgtttgacat agttattgat catggcagca gtcaatactc ttcgaaaatc aacattatcc
    14041 gttgtcaact tgccttgatg aaccccagtt ctatcatcgg tttcgtttcc taggctactg
    14101 ttgattttca ttgagtataa acttttctgt tcaaggcttt cctgagaatt gactacttga
    14161 ttaacataca gcagaacttg gtccaaatac aaatctaaat taggtaattc atgccagtag
    14221 gggatggtct gtttcatgat tatccacttt cttttatcta gtaatgataa ctagattgta
    14281 cgatatccaa tattaaaagt caagaaaaaa ccttctggac ccaaaatcac agaaggttag
    14341 ttgcttatgg ttggataata ttttctggta aagccatctt atagagtgaa aaggcaccat
    14401 ctaggttttt aacaccaaag cccttttctt tcaagattcg ttcggcaata taagaacgaa
    14461 gtccactatg gcagatgaca atgtattctt ttgaagcatc cagttctcca attcgctcac
    14521 ggagttggtc caaaggaatg tgaatgaatt ctctgaattg cccatttgcg acctcaccag
    14581 gattgcggac atctaataga actttaccag tagctaactc gtcgtctagt tgatgccact
    14641 ggatgttttg gctaatccct tctgtgatgt tcatagcagc gtagccaatc atattgacag
    14701 ggtctttggc agagccaaat ggcggagcgt aggtcaattc tagctctggt aaatcaaaaa
    14761 ttgtcagtcc agccttgata gcagtggcaa ggacatcaat ccgcttatca ataccttttt
    14821 gaccgacggc ttgcgctccg aaaatttttc catcagtcgg tgagaaaatg agttttaaaa
    14881 caatatcagt cgcaccagga taataacctg catggtcttt tccagttgta tgaataatgg
    14941 catgctcata gccgagtgat tgaacagtac gttcgcttaa accagttgag gcggcagcta
    15001 ggtcaaagac tttaacgata gctgttccaa tagaaccttt gttttttcta tcaagtccag
    15061 cgataacatc cgccacctgt ctgccttgtc ggttggcagg agaagccaga gaaatcaagg
    15121 catcttgacc tgtaatttct tgtttaacaa caatagcatc accaacagca taaatattat
    15181 tctgactggt ttggtagttt ttatctacga gaataccacc acggagaccc agttcaattc
    15241 cagttttttt agccagttgg ctgtcgggag caactccgac tgagagaatg gtcacatcag
    15301 atgacaaggt ttgtccgtta gacaattcta gaacctgtcc tttttcatga aaagcagtaa
    15361 cagactgccc agtgataacc tcgatacctt tattggttag ctctgcttcc acatatcgtg
    15421 ccatttctaa gtcaaaagta ggtaaaacat ggggagcttg ttcgacaagc gtgaccttcc
    15481 agccttttaa agctaagttt tccgccattt ccagaccgat aaaacctgca ccaattacca
    15541 caacatgacc agacttgatc tggtctagtt taatcataat cttgtccaaa tcaggtacgt
    15601 tccgaagagt aaagagattg tttgcttctt caataccaga tagatttgga atgacaggct
    15661 tggcgccggg tgataagatt agtttgtcgt agctttcaat caactcacca gtaggcgttt
    15721 tgactgttac agtttgctgc tcagcatcga tgctaataac ttcatggaag gggcgtacat
    15781 ctagattaaa gcgttgtttc aaactttctg gcgtttggag gagcaagcta tcatgttctg
    15841 ctatttcacc agaaatgtaa taaggtaaac cgcagttggc aaaggaaaca taaggtcctt
    15901 tttcgaatac gatgatttca gcatcttcca taagacgacg cagacgagta gctgctgaca
    15961 taccaccagc gacaccacct acaataatta ttttcatgct attcctcctg tccaggcatt
    16021 cataccgcct ttaatgttga ttgcttgata gccttgagat ttaagatatt ctgtcgcccg
    16081 ttggctacgc atgccagatt gacagatgac aagatagttc ttggttttgt cgcctttaaa
    16141 ttggtcaagt tgacttaaag gctggttgat agcttgtgga atatgtccac tagcaaattc
    16201 gtcgacttcg cgaacatcaa gcaaaatagc attcgatgat tgtatcgctt gttctacatc
    16261 agttatcgga agactatctg agtttgaaaa cattcctgaa aataattgtt taaacattgt
    16321 tactcctttt tactcctttt tatacccatg ggggtatcta aaatatatca aggaattcaa
    16381 aagatgtcaa ggtaaaaaat tgacattttt cacttcaagc agtactatat agggtatgaa
    16441 tactgataag aaaaagatga taaaccgttt gaaacgggcc gaaggtcaac tcagaggcat
    16501 tcagaaaatg attgaagaag atcaggaatg tattgatatt gtaactcagt tgagtgctgt
    16561 tcgttccagc attaatagta tgatgggaat ggttattgct cagaaggtac aagattgtat
    16621 cactcatcca tctgaaaacc ctgaagaaca agaagctcgt cttgctcaag ctatcaattt
    16681 aatcataaaa aaataggagc agaagtatgc caacagaaaa agaaaagatg atagctgggc
    16741 aattttatat ggcagcagat gctgaattga gagagatgcg aacctttgcc cgccaacaga
    16801 tgaaaaaatt taacgaagaa ctagatggac aagcccgctc tgagcgattg aaaaactggt
    16861 tgggagctac aggtgagcgg atttacgtgg agccaaattt ctactgtgac tacggtagca
    16921 atattcatgt tggtgaggat ttttacgcaa attttaactg tacgatgttg gatgtttgtc
    16981 ccattaccat tgggaaaaat gccatgcttg gtcccaatgt acagttgcta actccacttc
    17041 atccactaga tcctgatgaa cgaaattctg gtttggaata tggctctcca attaccattg
    17101 gtgataattt ctgggctggt ggtggtgtga caatcttacc tggcgtgacc ttgggaaaca
    17161 atgtagttgt tggtgctggt tccgttgtga ccaagtcttt tggtgataat gtcgttttgg
    17221 ctggcaatcc tgcgcgtgtt atcaaagaaa tacctaaaaa ataattcttc ttcgggagaa
    17281 ttttttcttt gccttgcgaa tagactaagt gaggtgaaga aaatgaataa ttttgaattg
    17341 tttaaatgga aaaaagcggg tttgaccaat cttaatgtga acaaagtttt ggcctatcaa
    17401 gaaaaacacg gtaaaaaatt gaccttgcga aatgtcgctg tagtgtctga atgtaaaaat
    17461 ccagttctat ttatggaaaa ttataagaat ctagatacaa aagcttgtcg cgaggaattt
    17521 aataagtatc catcattttc aattttagat gacatctatc ctttggcgct caaacagatt
    17581 tacaatccac ctgtactttt gttttatcaa ggggatttag aattgctaga aaaatcaaag
    17641 ctggcagttg ttggtacacg aaatgccagt aaaaatggcg ttcagtctgt tcagaagatt
    17701 atcaaagaac tcaacaatcg ctttgtcatt gtcagtggtt tggcaagagg aattgataca
    17761 gctgcccata ttgctagcct gaaaaatggt ggacaaacca ttgccgttat tggaacaggt
    17821 ttggataaga tatatcccaa agaaaataga gaattacaga cctacatcgg aaaacatcat
    17881 ttggttctga ctgaatatgg accaggtaca gaccctctca agtaccattt cccagaacgc
    17941 aatcgcataa tagctggttt ggtggaagga ttggtagtct gtgaagctaa gatgcggtct
    18001 ggtagcctca ttacctgtga acgtgccttg gaagaaggaa gagaagtcta tgctattcca
    18061 ggttccatta tggacggaaa atcagatggt tgtcatcatc tgattcaaga gggagcaaag
    18121 tgcgttacat ccggcttgga catcatttca gagtatcaat tttaaaacaa gtcttcctat
    18181 aggaaaataa atatcttgac actgactgaa aaatagtata ttctttgtga ggttaaaaat
    18241 atttgtaagg tataagaatg gctacaaaaa cagttagtaa gaaaaaaaca acaaccaaga
    18301 aaaatctcgt tattgtagag tcgcctgcta aggcaaaaac aatcgaaaaa tatcttggta
    18361 aaaattataa ggtcgttgct tcagtcgggc atattcgaga tttgaagaaa tctagtatgt
    18421 ctattgattt tgataacaac tatgcacctg agtacatcaa catacgtgga aaaggaccac
    18481 tcatcaattc tttgaaaaaa gaagcaaaaa atgctaagca agtctttctc gcgagtgacc
    18541 cggaccgcga aggagaagca atttcttggc atttggctca cattttggga ttggacgcaa
    18601 acgataaaaa ccgtgttgtc tttaatgaaa ttacaaaaga tgcagtaaaa aatgcctttc
    18661 aggagccacg tgctattaac ctagacttgg tagatgccca gcaagctcgt cgcgtcttag
    18721 accgtattgt agggtactcc atatcaccta ttttatggaa gaaagtcaaa aaaagtctgt
    18781 cagcaggacg tgttcaatcg gttgctctca aattaataat cgaccgtgaa aatgaaatca
    18841 gcaatttcaa accagaagaa tattggacaa ttgatgcaac cttcaaaaaa gggagcaaga
    18901 aattccaggc ttcattctac ggtatagatg gaaagaaaac taaaattgaa aacaatgagc
    18961 aggttaaaga aattctcgcc cgacttgatg gagatgattt cttagtagaa caggttgagc
    19021 gtaaggaacg caaacgaaat gcacctctac cttacacaac ttctactatg cagatggatg
    19081 cggctaataa aatcaatttc cgtactcgta aaaccatgat ggttgcccaa caactttatg
    19141 agggtgtcag tcttggcaca ggtggtaccc aaggtttgat tacctatatg cgtacggatt
    19201 caacacgtat tagccctgtt gcacaagcac aagctgctga ctttatcacg gagcgttttg
    19261 gtgctaccta ttcaaagcat ggtaataaag taaaaaatgc cagtggtgca caagatgccc
    19321 acgaagccat tcgtccatca aatgttaact taacacctga atcaattgca aaatatctgg
    19381 acaaggacca gttgcgacta tatacactga tttggaatcg ttttgtcgca agtcagatgg
    19441 ctgtagcagt ttttgatacc atgagtgttc gtttgggtca aaatggtatt gtctttgctg
    19501 ctaatggtag ccaagtaaaa ttcgatggtt atatggctgt ctataacgac gctgataaaa
    19561 ataaaatgtt gccagatatg gaacaaggtg acacggtggt gcgcgcgtca acaaatccag
    19621 aacaacactt tactcagcca ccagctcgct actctgaagc aaccttaatt aaaacattag
    19681 aagagaatgg tgtaggtcgc ccgtcaacct atgcaccaac gattgaaacc attcaaaaac
    19741 gctattatgt taagcttgcg gctaagcgat ttgaaccaac tgaactcgga gaaatcgtta
    19801 ataagctgat tgttgaattt ttcccagata ttgtcaatgt caaatttaca gccgatatgg
    19861 agcaaaaatt ggatgatgta gaaatcggga aagagcagtg gcagaaggtc attgacggtt
    19921 tctatcagcc attcaaggta gaattatcta aggcagaaga acagatggaa aaaatccaaa
    19981 ttaaggatga accagctggc tttgactgtg atgtttgtgg acatgaaatg gttattaaat
    20041 taggtcgctt tggaaaattc tatgcatgca gcaatttccc agactgtcgc aataccaagc
    20101 aaatcactaa agaaatcggt gtgacttgtc cagtttgcca aaaagggcaa gtgattgaac
    20161 gcaagagtaa acgcaatcgt cttttctatg gttgcgatcg ctacccagag tgtgaattta
    20221 cttcttggga caagccagtc gggcgttctt gtccgaaatg cgaccactat ctcgttgaga
    20281 aaaaagttcg tggtggtggt aaacaagttg tctgtccaaa tggtgactac gaagaagaca
    20341 agattaaata agattaaact gctattctca agggaatggc agttgttttt tagagtcatg
    20401 tttagtttat cttagcttac ttcgacaagc gaggaaactt cgtctcctta ttttcaactt
    20461 taaacgaggc tggtcaaaaa gtccaaagtc acttctcagt gcagtggttg attgggttta
    20521 acaggccagt ggagtgaaac tgttcagtga actgtttcag cctgagctta gaaatgaaaa
    20581 aatcgaggta aaaaggttgg agataagatt tgcgaagcaa atcacagcaa tttgtgtttc
    20641 acattccaag tctgacctct acaactaaaa cgaactaaac ggcaatactg cttgaaaaaa
    20701 tgtttaaaaa attaaaaaaa taggagaaaa gtcttgcatt attgaaaata ctgtgttatg
    20761 ataattgagg tttgaaaaaa ctgacctaag acagtagggg agctggactc ataaacatcc
    20821 taccgaggca caaaacgatt attgagataa acgtatttgt actttcgtgt ctaggtttag
    20881 gtgcgatttt tttgtgccta gcccaaaata aaaacggagg taaaaaaatg agtgaagcta
    20941 ttatcgctaa aaaagcggaa ttagtaaatg ccgttgctga gcaaatgaaa gctgcaacat
    21001 ctatcgttgt tgtagacgct cgtggtttga cagtagaaca agatacagtt cttcgtcgta
    21061 acttgcgtgg tgcagaagtt gagtacaaag ttatcaaaaa ctcaatcttg cgtcgtgctg
    21121 ctgagcaagc tggtcttgaa ggtcttgcag aattgtttgt aggtccatct gcagttgcat
    21181 tctcaaacga tgcagttgct ccagctaaaa caatttacga tttcgcgaaa actgctgatg
    21241 ctcttgaaat caaaggtggt gctgtagaag gtaaagtttc ttctaaagaa gaaatcgaag
    21301 cacttgctac attgccaaac cgcgaaggca tgctttctat gttgctttct gtacttcaag
    21361 cgccagttcg caacgttgca tacgctgtca aagctgttgc agaaaaagaa gacgcagctt
    21421 aatgcgtcag agtgtagctg cttagtgcta caaactattt aaaaacctaa attggaggaa
    21481 aatcacaatg gcattgaaca ttgaaaacat tattgctgaa attaaagaag ctactatcct
    21541 tgagcttaac gaccttgtta aagctatcga agaagaattt ggtgtaactg cggctgctcc
    21601 tgtagctgtt gctgcagctg gtggtgctgc tgaagaagct aaagattcat tcgacgttga
    21661 attgacatct gctggcgaca aaaaagttgg cgttatcaaa gttgtacgtg aaatcactgg
    21721 tcttggtctt aaagaagcta aagagcttgt tgacggtgca ccagctatgg ttaaagaagg
    21781 cgttgcaact gcagaagctg aagaaatcaa agctaaattg gaagaagcag gcgcttcagt
    21841 tactcttaaa taagagtcac atcataatta gattgcggaa tccctattta ccagtctttg
    21901 agagcgataa gcgattgata gaaactgata aattgatttg gtttcctgcc aaaaatcctg
    21961 ccaaaaattc tttggcagga tttttgtttt aataaatttt cgggggtaga cattaaattg
    22021 tctacccctt tttttgcgtt ttggaggtaa ttatgcaaaa gaaggaacaa tcatcacgcc
    22081 aaatcgtgat gtgccatctc gtggctattt taggagttga tatagagaaa gcaactcatc
    22141 tggttgatga attggaacaa gtaggtttaa tccgatttga tgaattcgga aatgttggac
    22201 ttttagtctt ggagggataa tcatgaaacg tattaccgct aatcaatatc agacatcaga
    22261 gcgttattat aaactcccaa aagtcttgtt tgagagtgaa cgttataagg atatgaagct
    22321 ggaagttaag gtagcctacg cggttttaaa ggatcggttg gagttgtctt tgagtaaagg
    22381 ttggattgat gaggatgggg ctatttattt gatttattcc aattcaaatc tgatggcact
    22441 tttaggctgt tcaaagtcaa agctactctc tatcaagaaa accttacgcg aatatggctt
    22501 aatcgatgaa gtccaacagt cctctagtga aagaggtcga atggcaaata aaatttactt
    22561 gggggaatta gaacatgaaa ctaccccagt cttacataca gacggggcta gtgttaaaaa
    22621 aacactaggg gggtctcaaa gaaagacggg gccggtctta tattcagccc ctagtgagac
    22681 tgaaggaagt gagactaaat atagtgaaac taaagggagt gatttcctta ttgaggacga
    22741 ggaggagagg cagttagtag atgagaaaca agaagaaaac tttacttcaa aagtcgatgg
    22801 cgtgaccaag tacgatcgag actatatttg gggtttggtt catgatcagt taagacagac
    22861 aggtctatct cagtcggcta gtgactatgc catgctctat tttagtgacc gttatcagta
    22921 tgctttggaa catatgcgat ttgctcggtc agcggaagta atagctgaat acgtatttaa
    22981 tggtgtgctg tcagagtgga ccaagcaact gagacgacaa gaagtaaaag gaggttaata
    23041 agatgatttg gtggatacta ggtggaatct atctgatttc tatcgtcatt ttgattgttg
    23101 aaatcatacg tgcaccagaa atggatgatc atatataagc aagagtttta caagtgtaag
    23161 gctctttttt agttggaaag agaggaaaaa tgaaattttt agatttattt gctggaatag
    23221 gcggttttag gctaggaatg gaatcacagg gccataaatg cctgggcttt tgtgaaattg
    23281 ataaattcgc cagaacatct tataaagcca tgtttaacac agaaggggaa atagaatacc
    23341 atgacattaa agaggtcaca gaccatgact ttagacaatt tagagggcaa gtggacatca
    23401 tctgcggggg atttccttgc caagcatttt cactcgcagg cagacgattg ggatttgaag
    23461 atactcgagg gactctcttt tttgagattg ctcgagcggc caaacaaatc caaccacgtt
    23521 ttctattttt ggaaaacgtc aaaggcctac tcaatcacga cgagggacgg acgttcgcca
    23581 ctatcctctc cacgatggat gaattggggt atgatgtcga atggcaggtg cttaacagta
    23641 aggacttcca agtcccgcaa aacagagagc gggtctttat tatcggacat tctagaagat
    23701 accgttccag attcatattt cctctcagaa gagaaaacag cccagctcat cttgaaaggc
    23761 taggaaatat caatccctct aaatgtggtt tgaatggtga agtctatctg acgagtggac
    23821 ttgctcctac actaacaaga ggtaaaggag agggagcaaa aatcgccatt ccagtcttaa
    23881 caccagatag acttgaaaaa cggcaacatg gtcgtcgatt taaggacaat caagacccta
    23941 tgtttacttt gaccagtcaa gacagacacg gagttgttgt cgcaggaaat ctgccgacta
    24001 gctttgacca gactggaaga gtattcgata tatctggctt gtcaccgact ttgaccacca
    24061 tgcaaggtgg agataaggtg ccaaagattt tgctgaggga ggagctgcca tttctgaaaa
    24121 tcaaggaagc cacaaaaaca gggtacgcaa aggcaactct tggagactct gtcaatctgg
    24181 cttatccaga ctcaaccaaa cgtaggggac gtgtgggaaa gggaatatcc aatactctga
    24241 cgacttcaga caatatggga gtggtggttg ctgctctgga atatcgacag gataagtggt
    24301 atgaagtcac aggcattgtc ttagagggga aagtttatcg cctgagaaca agacgactga
    24361 caccaagaga gtgtttcaga cttcaaggct ttcctgattg ggcttatgaa agagcagaaa
    24421 gtgtttccag taagagccag ctatacaaac aggccggcaa tagcgtgact gtcacagtta
    24481 ttgaagccat tgccagagaa tttagaagaa cggaagagga agaaaaacat gaacttacta
    24541 cataagaaaa gtatcctaga ttgtactgaa ttagaagaac gtattcacca agctgaaacc
    24601 aatcagctat tacaaaagat actgtcgctc cccaattttg attgtgactt tgaggtgact
    24661 tttgaagatg attaccacaa agagatgaat gatcccctat tctacgaatc caatcttcat
    24721 cagatttcgg attttatgga aactagggat attaaaaatg gtgtagatac actactgacg
    24781 aaagacaatc acctggcctt tcgtgccttt ggtgaaaatt attctgctag aggaaaggat
    24841 ggcattttaa cgactttagt gatagtcaag tgctttggtg aaggacggat gcccattgat
    24901 atgagtcgct atttctcaac tcctgaacca acagttgaaa atagcctaat cctataagga
    24961 ggtgcctatg cttgaggtat atctaggaaa taatgccaat acaaatcaag atttactaac
    25021 cattttgacg acctatggtg tgacttatcg ttgtataaaa gcgtgtgatg tagaccgtga
    25081 aatcttactg tcactctttg ccaaaacaat ggattgtttt gagttattgt cgccacgatt
    25141 tctttgcttt aagcgtcaat attcgataag tttgaatgag atgattcagc ttattctcca
    25201 aaagccagac cagaatcttc ggttgcccct cattgtctgt cagaatcatg tctatccagc
    25261 tattgggcta gacgaagtgc gtacctttct tcccagacaa gtaaaagaag agctgtttca
    25321 ggccagtctg atgaaacagg tgacagggta ggtgtgaaga tgaatgaacg attttgggac
    25381 aatttagaga tcattctggc cgaaaaagaa ctcacttggg cagaactagc acgtaaagta
    25441 ttcaacggtc aatatgttta tccaagtgag tttaatcgtc tctatcaaaa actgcgacat
    25501 tacaaatcca atcgtctgat gccacaaaca agatgggttg agcgtattgt tctagtctta
    25561 gaaattgatt atgaagattt atttaagagg tgacaatgat aaggatagtg gtgttttatc
    25621 tagctataca gcttaacggt cttctggtga gtttatacct gaaagagtat ctgacaatag
    25681 agggtatagt cttgctacaa ttggtcctat taagtgtgac ttgcttagag attgcccgtc
    25741 ataaaactgt tcaagcaaaa aatataacct tgagaaatcg cctaagatgg ttgcttcttg
    25801 gctttgtggc tatggttgct tttgcagtct tcatcagctt cctatttcca ggtcagacta
    25861 ggaatcaagc ggtattggta caagtaggaa aacaggttcc ccctattatc tttttattgt
    25921 ttctggtcaa tgcaagtatc cttgaagaga ttgtttatag gcaattgctg tgggaaaaat
    25981 tgacattccc ttttgtacaa ataggcgtga ccagttttct ctttgttcta tcccatggcc
    26041 ccaatcagtt agggagttgg ctcatgtata gctgtcttgg cttgaccttg gctgctgttc
    26101 gattgaaagc tgattgtatg acggcaatcg ccttacattt actttggaat agtttggctt
    26161 atgtcgtaac tttcttgtga taccaaaatc aagagtgctt ccgtattatg gaagcacctt
    26221 atgtttgata gggaatcgaa aaaaagagga ggtcaccatg tcatctgaac aacaagaacg
    26281 tcaagcgatg caatacgtgg aaagaagtag tttattgaca gttagaaccc tcttaaagct
    26341 cttagagtgg tcggctaggc aagccttggc tcaagattca gcttataaga ttggggttca
    26401 aaaactggaa gaactccttc aaagccctta cgtcattgaa gcgattgatg ttagtaaaga
    26461 cattctagat aagccaatcg atgtggagaa atttaaggag ttgatggagt cagaaaaact
    26521 gccaatcgca attagttggc aaaaagacta tctctacttt tatgccaagg ataagacctt
    26581 gctcgatcat cacttggatg aattgttgaa gaaactgatg tctaatccag agaagctaga
    26641 aggtttaacc tttgataaaa ccttggacca agagatagaa ctggccaaag agaaaattag
    26701 agtgacagaa ccttcggcag tcaaaaccaa ggaggtgacc ttgtaatgta ttcaagacga
    26761 aaagctttag tctttggact cttgggtctc gcctttggtt atttctgcca tcgtctaacc
    26821 ctgctctatg atagtttaac caatgcccca cctatggaac gttttgccta cctcttagga
    26881 gaggggctaa atcaagtctt caatccttta tggctatttt cctttactca aaaatctctt
    26941 cttgccttta tccttggggt tctaacgatg acattagtct atctttatgt atcgacagga
    27001 caaaaggtgt atcgagaagg ggaagaatac ggttctgcac gatttggaac cagtaaggaa
    27061 aagtggaact tttacagtaa gaatccttta aatgacacga ttttggctcg tgatgttcgt
    27121 ctaaccttgt tggaaaagaa gaaaccccag tttgaccgaa ataaaaattt gattgtcatt
    27181 gggggttctg gggcaggtaa gacctttcgc tttgtgaaac ccaaccttat ccaacttaat
    27241 tgttccaata ttgtcgtaga tccgaaagac catttggctg agaagacagg taaactcttt
    27301 ttagagaacg gttatcaggt caaggtttta gacttggtta atatgaccaa ttcggacggt
    27361 tttaatccct ttcgttatgt agaaacagag aatgatttga accgcatgtt aacggtctat
    27421 tttaataata ccaaaggaaa tggttctcgc agtgatccat tttgggacga ggcttccatg
    27481 acattggtga gagctattgc ctcttatttg gtagattttt acaatcctcc aggaagttcc
    27541 aagcaagagc aggaagcaag acgtaagcgt ggccgttatc cagccttttc tgagattggg
    27601 aaactcatca aactcttatc aaagggagat aatcaggaca aaagtgttct tgaagtcctg
    27661 tttgaagatt acgctaaaaa atacggtcat gagaacttta ccatgagaaa ctgggcggat
    27721 tttcagaact acaaggacaa gaccttggat tcggtgattg cggtcacaac agctaaattt
    27781 gccctcttta atatccaatc ggtgattgat ttgacgcaaa gagacactat ggatttgaaa
    27841 acgtggggca ctcaaaagac catggtctat cttgttattc cagataatga cactaccttt
    27901 cgtttcctat ctgctttatt tttctctacg gttttctcca ctttgaccag acaggctgat
    27961 gttgacttta aagggcaact gcctattcat gttagaagct atctggatga gtttgcgaat
    28021 gtcggagaaa tcccagactt tgctgaacaa acctcaacag ttcgctctag gaacatgagt
    28081 ctagttccaa tcttacaaaa tatcgcccaa ctccaaggac tttataagga aaaagaggct
    28141 tggaaaacta ttctgggaaa ctgtgatagc ctcctctatt tgggtggaaa tgacgaggaa
    28201 actttcaaat ttatgagtgg tctattaggc aaacaaacca ttgatgtcag aagtaccagt
    28261 cgttcttttg ggcagactgg ctcaagttct acctctcacc agaaaattgc ccgtgacttg
    28321 atgacggctg atgaagtcgg gaatatgaaa cgagatgagt gccttgtacg cattgcaggg
    28381 gttcctgttt ttcgaaccaa gaaatatttt ccacccaaac ataagaattg gaaatggctt
    28441 gcggataagg aaaccgatga acgctggtgg cactatcgca tcaatcccct aaccgctgag
    28501 gaagagctag aattgtcagg ccataaaata agggatttaa gcacagaaac gacactacat
    28561 taatagaaat gaggaattat atgaatcaaa aactatcagg ctttgtttac ggagtggacg
    28621 ctagctccat gttctcccaa gccatgtctc tattacaaaa aggtttgatt gcagtaggag
    28681 cctttcttgt tgtcatgggc attatcaacc tttcaaccaa cattaaagat ggtgggccag
    28741 gtgtccgaaa tgccattcta gaaattgttg gtggggtcat ggtgggagcc gcgggtactt
    28801 ttgtgactca gattaccatt taggggagga cagcacatga catgataatg aattttacgt
    28861 catcttttgt atatctagcc tctgaaaaag tgtctagcga tagccttttt gaagggtttc
    28921 aagtggattt agaatcaacc gccaacttgg ttaagtcact agcggacttt aatccgacgg
    28981 tttggtctta catgacagcc attaccaagg ggattatgca acccttggga gtagctattc
    29041 ttgcggttgt tctcgtactc gaattttcaa aaatggccaa gaaaatcgca aactcaggtg
    29101 gtgcaatgac ctttgaagct atggcaccta tgattgtcag ctacatcatg gtcgcggtcg
    29161 tgattaccaa tacgacggtt attgtggaag ccattattgc cattgcctct tatgttattg
    29221 aacaagtggc agagcttgtc acgaatggtg gtgctaatta tgataccatc tcaggcatta
    29281 agggatctgg aattgtaggt aaaatgatta ttggcttttt tgcgattctc atttggttgg
    29341 tacgaatggc cagtatcatg gttgtcaata tcttgattac cattcgcttt atacaactct
    29401 atctgatgat accctttgcc cctgttacca ttccgacctt tcttagtgat gactggagaa
    29461 gtgttgggat tggttacctt aaaaacatca tggtctatgc cgttcaaggt attttgattt
    29521 ttctaattgt atctcttgtt cccttgtttg aatcggctgg aaagattgcc gtatcaaatg
    29581 gagctggagt gatggaatca ctcgccatta tgtttggtgg cttggtacag gctatattgt
    29641 taatcattgc cttagttggc agtcaacgaa ccgcccgaag tattttgggg atgtaggagg
    29701 aagggagtca ttatctcgct tcctcttttt atagcttact tagattggag aacatttatg
    29761 aacactcgtg tctttaagga tatctcaaag gttcaacaca gggcatggct aggatttaca
    29821 actagacagg ttatttttgt atttccagct gtcgccctaa ccattttggt actgggcttg
    29881 aacctatttt attggcaatt tggggattgg tttgtctatg gttttgtttt tagctttacc
    29941 attccactca tgttatttgg ggtctatcgc cctaatgatt taccatttga aacctacctc
    30001 aagtaccgtt ttcattatga actaacagta ccagatcgta catttactgg aagaaaagga
    30061 gaccaacgtg aaaaaattaa aacacttaat gaaaccaaag acctcttcta atgacaaaaa
    30121 gcaaaagacg aaaacgcaga agcaggagat cagaccttct accgtaaata cgttagccta
    30181 tcaagggctt tttcagaatg gccttatgca ggtcagtcca agctatttct cacaaaccta
    30241 tctcttagga gatgtcaatt atcaaacagt tggcttagat gataagggag ctattgttga
    30301 gaaatattcc gatttaatca attcactgga tgataagact aatttccaac tgaccatcct
    30361 caatcaaaag gtcaatctgg aaaaattccg taagagtatt ctctatccct tacaggaaga
    30421 tgggtttgat gcttatcgtg atgaattgaa tcgcatgatg aatgccaact tagaggcggg
    30481 tgagaacaac ttttcagctg tcaagtttct ttcatttggc aagagcgacc aaacaccaaa
    30541 actagctttt cgttctctat cacagattgg ggaatatttc aaaagtggct tttcagagat
    30601 tgatgtatct cttggtttac ttggtggaga ggagcgagtg aatgtccttg cggatatgtt
    30661 acgaggtgaa aatcatttac cgttttctta caaggatttg accctctcag gtcaatccac
    30721 caaacacttt attgccccaa cctacctttc ctttaaacac aagaatcata ttgaattgga
    30781 cgatcgatta ttacagattg tttatgttcg tgactatggc atggagttag gggataaatt
    30841 tattcgtgac ctcatgcagt cagatttaga agtgatgatt agcctacatg ctaaaggctc
    30901 taccaagtct gaaaccatga ccaagctgcg aaccaagaag accttgatgg aatcgcaaaa
    30961 gattggggaa caacaaaaga tggctcggac tggaatctat ttggagaagg tcgggcatgt
    31021 tcttgaaaac aatattgatg aagctgaggc tcttcttcaa accatgactc aaacaggaga
    31081 taaactgttt gacaccgtat ttctaattgg cgtgctggcg gatactgaag accaactcaa
    31141 acaatctctt gatattatca agcaagtggc tgggtcaaat gatatgatta tcgataactt
    31201 gacctatatg caagaagctg cctttaatag tctcttgcca tttgggaaga actatctcga
    31261 aggtatttct cggtctctat tgacttcaaa cattgccgtg aatgcccctt ggacttccgt
    31321 agatattcag gacaagggtg ggaaatttta tggcatcaat caaatctcaa gtaatatcat
    31381 cagtattgac cgtggcaagt taaatactcc gtcaggtttg attttaggga cttctggagc
    31441 tggcaaaggg atggcgacaa aacatgaaat catctcaact aagctcaagg aagcagatag
    31501 tgatactgaa attattattg ttgacccaga aaatgagtac agtattatcg gtcaagcctt
    31561 tggtggggag agtattgata ttgccccaga ttctaccacc ttcttaaatg tcttagatct
    31621 atctgatgag aatatggatg aggatccggt taaggtcaag tccgaatttc tcttgtcttg
    31681 gattggaaag ctcttggacc gtaaaatgga tggtcgtgaa aagtccttga ttgaccgtgt
    31741 gacaagactc acttataagc attttgacac accgtccttg gtcgaatggg tctttgtctt
    31801 agctcaacaa cctgaacagg aagctaagga cttggcactg gatatggaac tctatgtgga
    31861 agggtcgctg gacatatttt cacatcggac caatattaaa acagacagtc atttcctgat
    31921 ttacaatgtc aaaaagttag gcgatgaatt aaaacaaatt gccctcatgg ttatctttga
    31981 ccaaatttgg aatcgggtgg tcaaaaacca aaagctaggg aagaagacct ggatttactt
    32041 tgatgaaatg cagcttctct tattggacaa gtatgcctct gatttcttct ttaaactttg
    32101 gagtcgtgtc cgtaagtatg gggcgatacc gacaggcatt actcagaatg tagaaacgct
    32161 tcttttggat gccaacggca gacgcattat tgccaatagc gagttcatga ttcttttaaa
    32221 acaggctaag agcgatcgag aagaattggt gcatatgctt ggactttcta aggaactgga
    32281 gaaatacttg gtcaatcctg aaaaaggtgc tgggctaatc aaggccggtt caaccgttgt
    32341 tccatttaaa aataagattc cacaacacac caagctcttt gacatcatga gtacggaccc
    32401 tgaaaaaatg aggacatgag atgaaagagg ataaaaagct agtcaaacat gctaggcaaa
    32461 actttcgaag caacttaaaa tcagcccgta tgcattatcg gaaagaagtt agagccttaa
    32521 aacagactgt tcctaaaaaa ggacgatttc gaaaaccagc ccaaaattct ctttttcagg
    32581 aaaagaaaac agagataaaa ggaaatctac tcagtagcca aaaggaagca gaagagaagt
    32641 tcctaaagga aattacctat gtttctccta gactgttgaa ggtaaaggaa atcaagaact
    32701 atcgacttcc tcaagctcaa gagcgtttgc ggactgcaag aaaacatttg tcagaagtga
    32761 aactaagtga gaagcaaaag gcagttaatc ccaagtttac tttccaaaaa gaaaatcctt
    32821 ccccgaagtc tcgctttcag tttcaccaag aaaaatcatt tgatcgacta agtgcagaaa
    32881 aagacgtaag ttctgccaag cgtgaggtta aacaactcaa gaaagtccaa aagactaaga
    32941 aaaactctac caaagtcaaa gttggattag gcttagttgc atctgaatcc cttgacctga
    33001 tagcacagga ggatgattta gatggtctaa gatccatgaa ggatattagt ttgaaagcca
    33061 gacgctatgg aaggtttacc tttcaagcag gtaaggtggt agtaaaaagt ggacagacag
    33121 gtgtgaggtt taccaaaaca aaattttctc atggaaagga acgattccag aacttcaaaa
    33181 agggaaaagg attcacacgc cagaaacctc ttaaaccaag aagacgttac cagacctttt
    33241 taaagcatgt gagaaaacaa agtgtcgcag gtatcaaagg aatcgtccaa gccattaagg
    33301 gaagtgtgac tttcttttct gtccttgcgg gaaatccttt gacttggatt gtctcaggca
    33361 ttctcttgat actccttttg atgatgagct ttttcatgag tgtttcaggt agtagtgtca
    33421 ttcaacaaga tgagatggag ttgaccaagg cctataccca catgacgtgg gaagatgcgg
    33481 aacatacgag aaccaatgaa aaagggattg cctactacac caagattgat gagattatgg
    33541 tttacatgaa tcatcaatac caagactaca agcttgacga tttcatggaa acaggtggtg
    33601 tgacctacaa agcgtttctc agtcaagtgt ggacagactt aaatggtgga gattctatta
    33661 aatccatgtc tgacttatat aaagaaccta cttacaagct gtctgatgag gaccaggaag
    33721 agttaaagga gttgacagaa ggaggcaact atctagccct tcaagaattg gacaatccct
    33781 ttcagggaca gactgacgag gatagcttaa gcatgaccta ccgatatggg tatgaggtca
    33841 ttgatgagaa accaacgctg catcaccata tcatcttaga agcgaaagaa ggtcaagtca
    33901 ttgtagctcc gatggatggc aaggtatctc ttgatggaga gaacgttgtt ttgacatctg
    33961 gtaagggagt gaataagact aaactaacct tgtttggcat tcattcaggc cgagtgagtg
    34021 aacatcaaca tgtcttggca ggagatatta ttggtcagac caaagacgga acgggtctaa
    34081 aagtcaccta tcaaaaggtt gatgatgata cggataagtt ggtctatgtc aatccagctt
    34141 tctactttcc aaaagtaatc caggttcaga ccaccattct tccaactatc ggtcagtttg
    34201 gcggcgatga gttcgagaga gccaaggcaa tttatgacta tcttaaaagc aaaggtgcga
    34261 caaatcaagc tatagcagct attttaggaa attggtcggt agaatcttcc attaatccaa
    34321 aacgagctga aggcgactat ttatctccgc cagttggtgc gactgatagt tcgtgggatg
    34381 atgagggctg gctctcactt aatggtccaa ctatatacaa tggacgttac ccaaatattc
    34441 tcaaacgtgg tttaggctta ggccaatgga cagataccgc ggatgggtca cgcagacata
    34501 ctttattgtt ggaatatgcc aaaggaaaac atcaaaagtg gtatgactta gagttacaac
    34561 tggatttcat gttgcatggg gacagtcctt attacaccaa ctggttaaaa gatttcttca
    34621 aaaatacagg tagtccagct agtcttgccc aactcttcct catttactgg gaaggaaatt
    34681 caggggataa gctacttgag cgtcagacac gggcaagtga gtggtattac caaattgaaa
    34741 aaggctttag tcaacccaac ggtgggacag cacaaagtga tccaaaagca cttgaagctg
    34801 tacgaggaga cctttttgaa aactctattc caggaggtgg tgacgggatg ggttacgctt
    34861 tcggccaatg tacttgggga gtcgcagccc gtatcaacca actggggcta aaactcaaag
    34921 gtaaaaacgg tgagaagatt ccaattatca gtaccatggg caatggccaa gattgggtac
    34981 gaacagccgc aagtctcggt ggggagatag ggacaagtcc acaagaagga gctatccttt
    35041 cctttgcggg aggaggacat ggcacaccaa cagaatacgg acatgtggct ttcgtcgaaa
    35101 aagtctaccc agatggctcc ttccttatct ccgaaaccaa ctacaatggc aatcccaact
    35161 acaccttccg taaattatct ggagtggata gtagcttgag ttttgcttat acgacgaaat
    35221 aaaaaaagga tatgctaaca aaatgaataa atgttagcat atcttttgtg tttttttgtc
    35281 acgattaatc agaatttata gaaatgagaa ctgttatgag acgaatcatc tcatcaagaa
    35341 catctgcaag tgaaatccct tttgtatagc tatatttttt tgaataattt tgccattgtt
    35401 tattctgaat agggtcagat ttgaacctct cgagtagttc aataatcttt acaaaatcga
    35461 gttctgtttc gcgatatgaa aatgttctct gacaggcatt tctcaactga atgaagtcaa
    35521 tgtcttcttt cttcagtttg gaaagaatat aaacatcgta aaaatctttg cttctactgt
    35581 tcaagaagtt acgtgaatag atggtttgaa gtttttcagc tagaattgtt tcaattgtat
    35641 aagcaatgat tggaaaattg tcttcatcaa aaatagcttt atagtcgtat gtaatcggct
    35701 gaggagttac aacatcacct gtcgcaatat ccaaatggat aacttgttta atattctcaa
    35761 gttggcacga aattgttgct cgatagccac catagtcatc actttcttta atagctgtga
    35821 ttgattgaat cacaaaagat ataccttctt cggaatctgc taaaatttcc ttgagttgct
    35881 gtttaacagt ttcttctgag agtgttattt gatggaataa aaagtcaata tcaacagtgc
    35941 tacgagattc aactccgatg acattcgaaa gtaggaatcc tcctttgaag atatagtgat
    36001 ttgaataaga actttgactt aattttttca aaatgacttc taggaagtaa taggtcatga
    36061 cggaattaaa ggttaaacca gtattctttg atattttatg acagagtgct gttaatttag
    36121 ctttgttcat attagaacct ccagagtttg tttaactttt tctaaagtat tcatttttgt
    36181 cgcatattca tagagttttg ctagattttt tttaggatag ttcccataag attgaagtgt
    36241 cttgacaaag agttcggaat caatcttttc tcgatgtatt acaaaatcac aaataatacg
    36301 ttcaaaatca tataccctta cgttgtttcc caaaggagta ggtacagtag tcattcccag
    36361 ctcgctgtat tctttagaga caaagtgaat atttagatta gctggaggag tattaaagcg
    36421 gtagcctcta ggaactgtca catcaaaata ttgtggaatt tcatctgtaa actgttgtag
    36481 atacagtgcc gaaatataag agaaaatagc cttaggaaaa cgatattgaa agaagtagta
    36541 ttcatcatag tctccgtttt gggttaggaa aatacctttt tcaactcgga agataatgcc
    36601 ttctttctct agtcttgtca gatatattgt tggaatacct agtgctttac aatctttgtt
    36661 tgtgacaatt ccattgttct tttctataaa atcgagtaga atctcttttt ttgacatacg
    36721 gcctcccact tgttgctttt acactacaat tttacaatat ttgtggtata aaagcaacat
    36781 tttcttttcc ctcattttat tgtgataaca aaatcaagcc ttctttttta tgatggtatt
    36841 gaacttgggc tacgagctgt acaaaaaaga gagtttctcc tagaacggaa agttcttcgt
    36901 caaaactcct atttttgtcg tgctcgttta acgccctcgt atctaataaa gagtgacagt
    36961 ttgttgctac ataaaaaaga aaagaaggac tatttatgac caaaacatgt aatcatcact
    37021 ttcttgtcaa tcaggaaaaa ggcgagaaac acgtctttcg taagagtaaa aaataccgta
    37081 ctctatgttc ggttgccctt ggaacgatgg tgacggctgt tgttgcttgg ggaggaacgg
    37141 ttgcacatgc tgatgaagtc acatcatcag ttgacaccaa catccaacga acggagaatc
    37201 cagccacaaa tttaccagaa gcacagccaa aacctgtatc tgaacaaact gaaattttag
    37261 cgttaactgg acaatctaat ggagcgattg ctgttaccgt accacatgat acggtaacac
    37321 aagcagttga agaggcaaag gctgaaggtg tttctacggt tgaagatagc caaatggatt
    37381 taggaaatac aacttctgcg gcagaaacta accaacaaat ttcaaaagca gaagcagatg
    37441 cccaaaatca agttgaggct atcaacgaag ttactgaaac ctacaaagct gacaaagcag
    37501 cttatgtgga tgaaaaagct cgcatcgaac aggaaaataa ggagctgtca caggcctacg
    37561 aaggggccaa ccaaactggt aaagagacaa atacttgggt tgataccaaa gtcaaagacc
    37621 taaaaactcg gtatgcagat gctgatgtga cagtaaaaga acaagtagtc tcatcaggaa
    37681 acggaacatc tgtactggac tatacaaact atggcaaggc tgttgaaact attcaatcaa
    37741 ctaacgaaca agctgtagcg gattatctaa caaagaaaac caaggcagat gatattgttg
    37801 cgaaaaatca ggccattcaa aaagaaaatg aagctggact tgctaaggca aaggcagata
    37861 atgaagccat tgaaaggcgt aatcaggcag gtcaagcagc tgttgatgct gaaaaccgtg
    37921 caggtcaagc cgcagtagat caagctaatc aggagaaaca acaattagtt tcagatcgtg
    37981 cagccgagat tgaagctatc acaaaacgta atcaagaaaa agaagccgca gccagaaaag
    38041 aaaatgaagt gattgatgcc tacaatacca aagaaatgga acgctatcaa cgtgacttgg
    38101 ccgagatttc gaaaggtgaa gaaggttata tttctgaagc ccttgctcaa gccctcaatc
    38161 tcaataacgg tgaaccccaa gcacaacatg gagcaattac ccgaaatcct aatcaaatca
    38221 tttcaactgg cgatgccatg ctgggtggct actcaagaat attggattca acaggattct
    38281 ttgtctatga tagcttcaaa acaggtgaga cccttagttt taactatcaa aatcttcaaa
    38341 atgcacgatt tgatggtaaa aagattagtc gagtgactta cgatattacc aaccttgtat
    38401 caccagctgg aaccgatgcc gtgaaattgg ttgtgccaaa tgatccaacc gaaggtttta
    38461 ttgcctatcg aaatgatgga aatggtgatt ggcgaacaga caagatggaa tttcgtgtag
    38521 ttgccaagta tttcttggaa gacggaacgc aggttacctt ctccaaagaa aaaccaggag
    38581 tcttcacgca ttcttccctc aatcataatg acattggtct agaatatgtc aaagatactt
    38641 ctgggaaatt tgtggcgatt aatggttcaa ccgttcaagt gactaatgaa ggtctagccc
    38701 attctttggg tttcaaccgt gcaagtgatt tgaatctgcc tgaggaatgg gataccacat
    38761 caagtcgtta tgcttataaa ggagcaattg tctcaacagt tacatcaggc aatacctaca
    38821 cagtaacctt tggccaaggc gatatgccac agaatgttgg attgtcttac tggtttgcct
    38881 taaataccct accagttgca cgtacagtga caccttatag tcccaaacct catgtagcgg
    38941 tggaacttga acccgttcca gaacctatta cggtaacacc agatgtcttt actccaaaaa
    39001 cctttacacc tgaaaagcct gtgaccttca cgccaaagcc tttggaagaa gtagtgcagc
    39061 ctagtctaac tttgaccaag gtaaacttac ctgttaaacc tattccaaaa gaacttccaa
    39121 cgccaccaca agttccgact gtccattatc atgcgtaccg tttgacgaca actccagaga
    39181 ttatgaaaga agtagtcaat agtgaccaag ctaatcttca tgagaaaact gtcgcaaaag
    39241 attcaacggt gatttatccc ttaacagttg atgccttatc gcccaatcgt gcccaaacga
    39301 ctagtctcat ttttgaggac tacttgcctg ctggttacct atttgataag gaaacaacac
    39361 aaaaagagaa tggaaactat gtccttagct ttgatgcgac taagaatttt gtgaccttaa
    39421 ccgcaaagga aaacttgttg caggaggtaa ataaagattt aaccaaggtt tatcaactga
    39481 acgccccaaa actctatggt tctgttcaaa atgatggggc aacctattcc aatagttaca
    39541 aactcctttt gaacaagggc acaacaaatg cttacacagt cacttcaaat gttgtaacgg
    39601 ttcgtacacc aggtgatggg gagacaacca cactcattac accagataaa aacaatgaaa
    39661 atgcggatag tgtcctcatt aatgacacgg tcgtagccct tggcacaacc aaccactatc
    39721 gtttgacttg ggatttggac cagtataagg gagatcgttc tgctaaagag acaattgcac
    39781 gaggcttctt ctttgtggac gattacccag aggaagtgct tgatgtggtg gaaaatggga
    39841 cggctgttac aacccttgac ggtcagaagg tatcaggaat tacggttaaa aactatgctt
    39901 ccctaaatga agcccctaaa gaccttcaag ataaattggc tcgtgctaag attacaccga
    39961 caggtgcctt tcaagtcttt ttgccggatg acaaccaagc cttttatgac cagtatgttc
    40021 aaacaggaac ttctttagct cttttgacca aaatgacggt gaaagatagt ctctatggtc
    40081 aaactaagac ctatacaaac aagtcttatc aagttgattt tggaaatggc tatgaaacca
    40141 aggaagtgac caacaccctt gtttctccag aacccaagaa acagaacctc aataaagaca
    40201 aagtggatat caatgggaag cccatgctag tgggaagtca aaattactat actctctcat
    40261 gggacttgga ccaataccga gggattaaag cagacaactc tcagattgca caaggttttt
    40321 actttgtgga tgattgtcca gaagaagctt tattgccgga tgaagcagct attcagttta
    40381 tcacatctga tggcaaaaca gtttcaggaa tcacggtgaa ggcttattct caattatcag
    40441 aagctcctaa aatgctacaa gcagcccttt cgaaacaaaa aattcagcct aaaggagctt
    40501 ttcaagtttt catgcctgag gacccacaag ccttttttga atcttatgtg accaaggggg
    40561 agaatattac cattgtcact ccgatgacgg ttttggaaac catgcttaat tcagggaagt
    40621 cttatgaaaa cgtggcttat caggtggact ttgggcaagc ctatgaaacc aacacggtga
    40681 ccaattttgt ccctaaagta actccacaca agtctaatac caatcaagaa ggcatttcaa
    40741 ttgatggaaa gactgttctt ccgaatacgg tcaattatta caaaattgtc ttggattaca
    40801 gtcaatacaa ggacatggtc gtgacggatg atgttcttgc caagggattt tacatggtag
    40861 acgattaccc tgaagaagcc cttaccctaa atcctgatgg cattcaagtt ttggataagg
    40921 atggcaatcg tgtatctggt atctctgtca gcacctacgc tagtttgtca gaagcttcga
    40981 atgtcgtcca agatgccatg gctaaacgtc agtttacaac taaaggagcc attcaggtcc
    41041 ttagtagcga tgatcccaaa accttttatg agacctatgt gaagactggt caaaccttag
    41101 ttgtcacgct tccgatgacg gtaaaaaatg agttgaccaa aacaggtggt cagtatgaaa
    41161 atacagccta tcagattgat tttggcttgg cctatgtcac ggaaacagtg gttaataatg
    41221 ttcccaaact agacccacaa aaagatgtgg tgattgactt gtctcataaa gatgacagcc
    41281 ttgatggtaa agaagtggcc atgaatcaaa cctttaacta tcgtttggtt ggagcgatga
    41341 tcccaagcaa tcgtgcgaca gatttatttg aatatggttt tgaagataac tatgatgaaa
    41401 agcatgatga gtataatggt gtttatcgta gctacctgat gacggatgtc atcctcaaag
    41461 acggttctgt cttaaaagag gggacagaag tcacgaaata tagcttgcaa caggtggata
    41521 cagaaaatgg cctagtgtca atttcatttg ataaatcctt cttagagact atctctgatg
    41581 attcagcttt tcaggcagat gtttacctgc agatgaaacg gattgcgact ggtcaggtgg
    41641 agaataccta ccttcataca gtgaatggct atgtcatcag ctcaaataca gttgtaacac
    41701 atacacctca acctgaagaa ccaagtccaa atcaacccac accacctcaa ccaccaattg
    41761 agactattga accgcctgtt ccagcaagca ttttgccaaa tacaggggaa caggaatccc
    41821 ttttgggctt gattggagct ggtattctac ttggtacggc ttatggactg aagaaaaagg
    41881 aggagaagta gatgaatcca aaaactattt atgaaaagga ttcagaccaa gatggtttga
    41941 cagatgctca ggaactagct ttggggacca atccgcagtc tgttgacaca gatggtgatg
    42001 gtcaagctga tttagaggag ctacaatctg gacattcacc actagtccca caaaaggagt
    42061 tgtgtgatgg cttggaactt tgacaccatg aaagaggctt tgtctgaaat ggaaaaggtc
    42121 aattaccaag agtttatcaa agcctttctc tctttggaac taagcatttc tgatagaaca
    42181 atcctcaatc aagtttatca agattatatg gatgaagatg acctgtcctt gattagtgat
    42241 gagctacgag ttaaagtgga aggttatcag gatgaagtaa aagctgatat gactgatatt
    42301 ctggaaaagc tgtaccgaac aggtgaaggc tctagtttta ttatggatct aatgtcctca
    42361 aatagtcttt cagacacctt ggaacaatat gaagttttgg atagtgacga ttattcacca
    42421 cttagccttg aaaccttaca ggccatgatt caacaggact tggccatttc cagtcaagat
    42481 tattttggag atctggttca ccttgccttg caaaaagatt tactagacca gaaaagtcat
    42541 ttcttacaat actatgtggc aactgtaatg gaaggtattc cacaagaaag agaccaacgt
    42601 gccttggtcc tagattaacg caaagggctg agaaacactc tcaacctttt ttaaatggga
    42661 gggaaaaatg aatcaagaag tcttactaca aatgatgaga gccaccattc ctcgtgatag
    42721 agccttgctt gaggcgtttt tatattacca agcagagcat tttgatgagg agtgggatag
    42781 tctcgtttat cagtttatga ctaataggca agaaataaat aagtctgttc aggtacttca
    42841 ttttgagaca gatgtttcag cttttgttca ggctagtcct tatgatactg ctcatgatct
    42901 attgacctat acacaagtat tcggccaaac tggtctccaa aaactcgaca aactatcgcc
    42961 gtctgaaaaa gacttggtga ttgaagtggc cttgtttaat ctggccacac gttttcaatt
    43021 attagattcc aatggtcatt accaaactat ctcaccagat tcgcttttac aaaagagtag
    43081 gggagccaat ttggtcaatg tttaccgtgt ggctaataat ttagcggaac gcattagccg
    43141 agatattgaa cagtttctct tgacttacga gcctgagctt gaaacgagag ttgaggaaac
    43201 agttcaagaa aaagaagaaa ctgttgatga gcacaaaaca agtgttcatc aagcaatatc
    43261 ttttcgagaa gagggctccc tggttattgc tagtttggat gtagatttgt ctcaactgga
    43321 tatccgaact ggaaaaacca gtcatctacc agcttatgaa gagttatctt tacgacgtaa
    43381 atttgagatt ctaacctatt ttgaccagat tcgaaatgaa cgttcaaaac ttccgaatct
    43441 tagacgaggt gattttgata ctgagatgga aaagacacca atctttgatg gcgaggagtt
    43501 acttacctat ctagaggctg atggcagcct ctatgagctg aaacggacac tgactacagt
    43561 cgaagaaaag gaattagaaa aaattggaca agccattagg acagaaaatc aagaaaaatt
    43621 gactcaggta gggattgatt tatctcagtt tgactctgac caaatcggta ttttattgga
    43681 tgccgcaggt cgttttcatc tgaaaaatgc ggatcttgca ttattaggtg gctatccaaa
    43741 tgcctctgta actcaactag cccttgcgac agaactactc caaatgggat tgagtcatga
    43801 aaaaattgaa tttttctttg gtagtcagct ttccattgaa gagctgcgac aagttgccta
    43861 cgccttttta caccaagaac ttagcataga agacgcggag caatttgaaa aagataaagg
    43921 caatcagcca gatttaaccc tcagagattg gaagaacaag ctagagaaag ctgagggaaa
    43981 agaagtagtt gatgaagcgt ttgaggtaaa tccacttgtt cagatggttt tggacacttt
    44041 tcctcttggg tcattagttt cctacaaggg acaggacttt gaggtcgttt cggtcagcga
    44101 tgctcgattg aacggtttga ttcggattga gttagtcaat gacttttcgg atatcattga
    44161 acaaaatcca gttctttatt tgaggacctg ggaagaagtt agtcaggcac ttcatcagcc
    44221 aaaggcagaa ccacaaacag agttagacga tgaagaccaa gaattaaacc ttttttcatt
    44281 tctggaagag gaaacaagtc aatctgttaa tgaagctgtt cagaacattg gagttttgga
    44341 tccagaacaa tcgggagaag ctagtcatga cagtgaaaag ccaaccattt atgtacctga
    44401 agaagtgctt gtagaatcga ttccagatat tccagcaacg gctttctact ttccagaaga
    44461 tatgacggac ttttatccta agactgctag agataaggtt gagacaaaca ttgcggctat
    44521 tcggttggtt aaaacacttg aaacggaacg ccgtcaagca agtacaagtg aacaagaact
    44581 ccttgccaag tatgtgggct ggggtggact tgccaatgat ttctttgatg actatagtcc
    44641 aaaattttct aaggaacggg aagaactgaa aagcctagtc acagataaag agtattcgga
    44701 tatgaaacag tcctctctga cagcctatta tacagaccct gcccttatcc gtcagatgtg
    44761 ggataagttg gaaagagatg gctttacagg tggcaaaatc ctagatcctt ccatgggaac
    44821 agggaatttt tttgcggcta tgccaaaaca cttaagagaa aagagtgagc tatatggcgt
    44881 agagttagat actattacag gagctattgc caaacacctt catcccaata gtcacattga
    44941 aattaagggc tttgagacgg tggcttttaa cgacaatagt tttgatttgg tgatttcaaa
    45001 tgtgcccttt gccaatatac gaattgcgga taataggtac gataagcctt atatgattca
    45061 tgattacttt gtcaaaaaat ctcttgattt agtccatgat ggtggacaag tagcgattat
    45121 ctcttccaca ggaaccatgg ataagcgaac agaaaacatc ttacaagata ctcgtgagac
    45181 aactgaattt cttggtgggg ttcgactgcc tgattctgcc tttaaggcca ttgcaggaac
    45241 gagtgtcaca acggatatgt tattcttcca gaaacactta gacaagggat atttggcaga
    45301 cgacttagcc ttttcaggtt ctattcgcta tgagaaggat agtcgcattt ggctcaatcc
    45361 ttactttgat ggagaataca atagccaagt gctaggaacc tacgaggtca gaaattttaa
    45421 cggaggaaca ctttctgtta aggggactag tgatgacttg attgcaagtg tccaaactgc
    45481 tctaaatcac gttaaggccc caagagagat tgatagaaat gaggtcatca ttaatccaaa
    45541 tgtgttgacc aaacaagtca ttgatacctc cattccagct gaaatgaggg agaatctggg
    45601 tcagtacagt tttggttacc agggttctac agtttactat cgagacaaca aaggcattcg
    45661 agtcggaacc aagatggaag aaatcagtta ctatgtcgat gaagagggca acttcaaagc
    45721 ttgggacacc aaacattccc aaaaacagat tgatagattt aataacttag atgtgactga
    45781 tagcactgct ctggatgtct atgtgaccga tgatgcagct aaacgcggac aatttaaggg
    45841 gtattataaa aagacagttt tctatgaagc tcctttgtct gataaagaag tggcacgaat
    45901 caaaggaatg gtcgatattc gcaatgctta tcaagaggtc attgccattc aacgccatta
    45961 tgactatgat aagaagacct ttaaccactt gttaggcaaa ctcaatcgta tctatgatag
    46021 cttcgtcaaa cgctatggat atttgaatag tactgtgaac cgcaatcttt tcgatagtga
    46081 tgacaagtat tcgcttcttg ctagtttgga agatgaaagt ctggatccaa gtggaaagtc
    46141 tgttatctat actaaatccc ttgcctttga gaaggctcta gtgcgtcctg aagaagaggt
    46201 taaaaaggta catactgccc ttgatgcctt aaattcgagc ttggctgacg gacgaggtgt
    46261 tgattttgct tatatgatgt ctatctatca ggttgaatcg aagatgactt tgattgagga
    46321 gttaggagac ctcattatgc ctgatcctga gaagtatttg aatggagaat tgacctatgt
    46381 ttctcgtcaa gactttctgt caggtgatgt tgttaccaag tcagaagtgg tggacctatt
    46441 cgtgaaacaa gacaatcagg actttaactg gtcacattat gcaggacttc ttgaagctat
    46501 caaaccagcc cgtattactt tggcagacat tgattatcga ataggttcac gctggattcc
    46561 tttgtctgtt tacggaaaat ttgcccaaga aacctttatg gggaaatcct atgaactgtc
    46621 agaccaagaa gtagcaacag tccttgaagt cagtcccatt gacggggtta tcacttacca
    46681 atctaagttt gcctacacct attccaacgc aacggatagg agtttaggtg tccctgcttc
    46741 acgctatgat agtggtcgaa aaatctttga aaacctcctg aattccaatc aaccaaccat
    46801 cacaaaacaa gttgtcgaag gagacaagaa aaagaatgtg acagatgtgg agaaaacaac
    46861 ggtcctgcgt gctaaggaaa cgcacctcca acaactcttt caagattttg tatcaaagta
    46921 tccagaagtt caacaaatga ttgaagacac ctataatagg cgttacaatc gtacagtatc
    46981 aaaggtctat gatggtagcc atttaaccat tgatggactt gcccagaata tttccttacg
    47041 tcctcaccaa aaaaatgcca ttcaacggat tgtggaggag aaacgtgccc tactagccca
    47101 tgaggtaggg tctggaaaaa ccttgaccat gcttggagct ggcttcaagt tgaaagaatt
    47161 aggaatggta cataaaccac tttatgtggt gccgtctagt ctgactgctc agtttggtca
    47221 agaaatcatg aaattcttcc caaccaagaa agtctatgtg accaccaaga aagattttgc
    47281 caaagccaaa cgtaagcagt ttgtgtcccg tattattaca ggggactatg atgccattgt
    47341 cattggggat tcacaatttg agaagatacc aatgagtcgt gaaaaacagg tcacctatat
    47401 caatgacaaa ctcgagcaac tccgagaaat caagctagga agtgacagtg attacacggt
    47461 gaaagaagcg gaacgttcga ttaagggatt ggaacaccag ttggaagaac tccaaaaact
    47521 agagcgagat acctttatcg aatttgaaaa ccttggaatt gattttcttt ttgtggatga
    47581 ggctcatcac ttcaagaata ttcgtccaat cactggactt gggaatgtcg cgggaatcac
    47641 caatactact tcaaaaaaga acgtagatat ggagatgaag gtgagacagg ttcaggcaga
    47701 gcatggcgat agaaatgttg tttttgcgac aggaacacca gtttctaact ctattagtga
    47761 acttttcacc atgatgaatt acattcaacc tgatgtttta gagcgatacc aggtatccaa
    47821 ttttgattca tgggttgggg cttttgggaa tattgaaaac tctatggaac tagccccgac
    47881 aggagataag taccaaccca agaaacggtt caagaaattt gtcaaccttc ctgaactcat
    47941 gcgaatctac aaggaaactg ccgatattca gacctcagac atgcttgatt taccagtacc
    48001 tgaagctaag attattgcgg tggaaagcga gttaacgcaa gctcagaaat actatttaga
    48061 agagctggta gagcgttcgg acgctatcaa gtcaggtagt gttgatccaa gtagagataa
    48121 catgcttaag ataacaggtg aagccagaaa actagctatt gatatgcggt tgattgaccc
    48181 tgtttactcc ttatcggata atcagaaaat ccttcaagta gtggataatg tcgaacgaat
    48241 ctaccgtgaa ggagctggag acaaagccac tcagatgatt ttctctgata ttggaacccc
    48301 taaaagtaag gaagaagggt ttgatgtcta caatgaactg aaagttttgc tggttgatcg
    48361 agggattcca aaagaagaaa ttgcctttgt tcatgatgcc aatactgatg agaagaaaaa
    48421 ctctctctca cgaaaggtca atagcggaga agtacggatt ctcatggctt ctacggaaaa
    48481 aggtggaacg ggtctaaacg tgcaatcacg catgaaagct gtccaccact tagatgttcc
    48541 ctggcgtcct tctgatgtag gacggatttt gcggacattc aaaataaaaa agaatgtgga
    48601 ggtaacagac aatggcaaag acaatttttg aggaaatggg cggcaaatac gaaaggcaag
    48661 gcgattattt aataccgtgc ttaactgtac ccgccgaaga agaacagccg ataggcatct
    48721 ggggacagcg gcatttggat tatctgaagc atcactgcaa agttacatac accaatcttc
    48781 ttacaagcgg cagacttaac gcttaccttg ccgacattga cagacaggca caggaacgct
    48841 ttgaaaggct catagagggt atgaaacagg cacagggcat aacggaacag ctaaaggcag
    48901 aaaacgcctt agaatggaca ggatgcctca ataacataag ggcttgtgcg agggagattg
    48961 tggaaaagga aattattttt gcataaacag atgattagtg gcagggggaa atcctgccgc
    49021 tttttctgct ttagtttgtc agcttgacaa ataaagggtt aaggaatata attagattca
    49081 gtattataca aggagttaat aaatatgcgg caaggtattc ttaaataaac tgtcaatttg
    49141 atagtgggaa caaaaagtag cagtcccgtt tcacttttaa tatggggctt agttttttgt
    49201 acccagttta agaatacttt tatcatgtaa ttttatatgc ccgaaaacat ataagtgttt
    49261 tggggctatt ggagttattt acccagtgat aggagtattt atcactgggt atttttatgc
    49321 ccttttttgg gtgttgatag gaggaaaatc acatgaaaat aattaactta ggcattctgg
    49381 ctcacgttga cgcaggaaag acaacattaa cggagagttt attgtatacc agtggtgcaa
    49441 ttgcagaacc agggagcgta gataaaggca caacaaggac agatacaatg aatttggagc
    49501 gtcaaagggg aatcactatc cagacagcag tgacatcttt tcagtgggag gatgtaaaag
    49561 tcaacattat agatacgcca ggccatatgg attttttggc ggaagtatac cgttctttat
    49621 ccgtattaga cggagcagta ttattagttt ctgcaaagga tggcatacag gcacagaccc
    49681 gtatactgtt tcatgcacta cagacaatga agattccgac aatttttttc atcaataaaa
    49741 ttgaccaaga ggggattgat ttgccaatgg tatatcaaga aatgaaagca aagctttctt
    49801 cggaaattat agtgaagcaa aaggttgggc agcatcccca tataaatgta acggacaatg
    49861 acgatatgga acagtgggat gcggtaatta tgggaaacga tgaactatta gagaaatata
    49921 tgtcagggaa accgtttaaa atgtcagaac tggaacagga agaaaacagg agattccaaa
    49981 acggaacgtt atttcccgtt tatcacggaa gtgctaaaaa caatctgggg attcggcagc
    50041 ttatagaagt gattgccagt aagttttatt catcaacgcc tgaaggtcaa tctgaactat
    50101 gcgggcaggt ttttaagatt gaatattcag agaaaaggcg gcgttttgtt tatgtgcgta
    50161 tatatagcgg aacattgcat ttgagggatg ttattaaaat atctgaaaaa gagaaaataa
    50221 aaatcacaga gatgtgtgtt ccgacaaacg gtgaattata ttcatccgat acagcctgct
    50281 ctggtgatat tgtaatttta ccaaatgatg ttttgcagct aaacagtatt ttggggaacg
    50341 aaatgctgtt gccgcagaga aaatttattg aaaatcctct ccctatgctc caaacaacga
    50401 ttgcagtaaa gaaatctgaa cagcgggaaa tattgcttgg ggcacttaca gaaatttcag
    50461 atggcgaccc tcttttaaaa tattatgtgg atactacaac gcatgagatt atactttctt
    50521 ttttggggaa tgtgcagatg gaagtcattt gtgccatcct tgaggaaaaa taccatgtgg
    50581 aggcagaaat aaaagagcct actgttatat atatggaaag accgcttaga aaagcagaat
    50641 ataccatcca catagaagtc ccgccaaatc ctttctgggc ttctgtcggg ttgtccatag
    50701 agccgctccc tattggaagc ggagtgcagt atgaaagcag agtttcactt ggatatttaa
    50761 accaatcgtt ccaaaatgcg gttatggagg gggttcttta tggctgcgag caggggctgt
    50821 atggatggaa agtgacagac tgtaaaatct gttttgaata tggattgtat tatagtcctg
    50881 taagtacccc cgcagacttt cggctgcttt cccctatcgt attggagcag gctttaaaaa
    50941 aagcagggac agaactatta gagccatatc tccactttga aatttatgca ccgcaggaat
    51001 atctctcacg ggcgtatcat gatgccccaa ggtattgtgc agatattgta agtactcagg
    51061 taaagaatga cgaggtcatt ctgaaaggag aaatccctgc cagatgtatt caagaataca
    51121 ggaacgattt aacttatttc acaaatgggc agggagtctg cttgacagag ttaaaaggat
    51181 accagccagc tattggtaaa tttatttgcc aaccccgccg cccgaatagc cgtatagata
    51241 aggttcggca tatgttccac aagttagctt aacagcttgc aaaagtcata taaaatgaga
    51301 tttgaaagga ttagagacta attatgatga aatgcgaatg gatattgtgt cctgtttgtg
    51361 ggagcaaaac ccgtaataaa attaggaagg acactgtttt ggagaattat cccctttatt
    51421 gtccaaaatg cagacaagaa agcttgatta aagttgacaa cttgaagata actgtcatca
    51481 aagagccaga cgcttaagac gcagagccga tgaaattgtg gaacaattca cgaatcatcg
    51541 gctctttttg tttcgtattt gaaagaacaa actcaccaaa taaaaaaaac gatattcggg
    51601 tgggttattt tgttataccc taaattaccc tctgaatttg tttttaaatt tggagggatt
    51661 tttttatgtt cttttttcgg gcggttattc atctgctcat acgaagtaaa atttatcaat
    51721 acatagcata tcagagaatg gcaggaaacc agttaaaaaa atttctgcca gtgcagcggt
    51781 acttctcacc ttgaaagtgg aagtacaatc tccatacaac agaatttgta tttcccataa
    51841 ccgtgaaggt cacagagcct ttgcggtttt tcttttgtcg ttttttgttg gaaagtgccg
    51901 cagggctgtt tctggtgtgc gttgccgctc cccgcctctc catctggatt tttacatttc
    51961 aaaaattcag attggaggta ttagcggtga aatacgcacc aagaaaagta tatatcaaag
    52021 aaagcggcgg ctatgtggaa ctgtcctata cggatttctg ccgccgcagg caagccgaca
    52081 agggatatat ggacaagctg tttatccccg tccaaggttg tctgcttgaa gtcgtaaggg
    52141 agcaatatgc ggacttctac cgtgacaggg agcggtggcg ttacctgcaa aaattagatg
    52201 cgaggaacag cctgctatct ctggacggat ttacggacag cgaggggaat cctctggact
    52261 ttatcgctga tgaagcggcg gacattgcgg aaaccgttgt caatgctgtt atggtggaca
    52321 ggctgaaagc cgccctgcct ttgttgtcgg atagtgagca ggagttgata caggcaatct
    52381 tttttgacgg actttccgag cgtgaagtcg gggcgaggtt aggcataacc cagagcgttg
    52441 tgaacaaacg caaagccaga atcctaagaa aactaagaaa gataatagaa aattaaaatt
    52501 taaggcgttc agcccccttg ttttttcctt tgggaaaatg agggggcttt tctctgccct
    52561 ctcaatcaat tctgattgga ggaagaaaga atggcataca accacggacg ggaggacagg
    52621 aaatggcgta tctggaaaga agcggaggaa aagctgctgc gtgagtgcgg cgttgatgaa
    52681 gtgaccattg agcagatacg catagcggac agggcagact tcaattccaa caggcggttt
    52741 taccgatgga cgaatgacgt tgcggaatac cttgaggaca tggcagacag ggagcggcaa
    52801 gcggaggtga atacagttgc ggagttactg gacgagattg agagcgaaaa tctctatcaa
    52861 gtattagtca cggtggacgg gcgtaccttg aaaatcgtcc tgctgaaaat gcaggggtat
    52921 tccacaaagg agattgcccc acttgtgcat ttgacgactg gtgccatcta tgcgaggtta
    52981 gaccatctgc ggaagaagct tcggaaaatt ttgtaagcgt ctaatacatc ccattattct
    53041 gcgggctact gggtggggag tggaatctcc ccgcttattt ttggcaggag gaaaacggga
    53101 tggcatataa agctaaggcg tacacgcttc gggaggaatc cacggaaagc ggtataaggt
    53161 attttatcag ttttaaggac gggcaggggg aacaccacga actggaagta tccgagcagt
    53221 tattctttga atttcggcag atggaacgca ggaacaggaa tcttctccaa tgggacgagc
    53281 ggcacaggga gttttcggaa gtatgggacg aaacgctgaa caggcgggcg ttaaagctgc
    53341 ctaagagcat tgaggaacaa atgattgaag cagaacgggc ggaactgctc tgtaaggcgg
    53401 ttgacagact gccagagata caaaggcgac gtttcctgct ctactacgag tatgagttca
    53461 atttttacca aatcgccgct atggagcatt gcaccgcttc tgcaatacag aaatccgttg
    53521 ctgttgcaaa gaagaaagta aaagcggaaa tgaggaaata tctccaacca tgaccgacac
    53581 cgcccgaaaa agaaatctgt tttttatttg tgtgggattg gcggtattac atactctcac
    53641 ttttttcgtg ggagtaaatc tatttttaag gaggtcaacg cctatgtgca acaaaaacaa
    53701 gataactgcc cgaaaggagg aaacccatgc agaagttaca gacagtcaac gccgaaacgc
    53761 tcctttatga gccgcttgag aagccgtcct ttgtggtgga cagccttatc cccacaggct
    53821 tgtcgctgtt ctgcggctca cagaagatag gcaagagctg gctcatgctg aagctgtgct
    53881 tatgcgtgtc gcagggaatc cccttatggg acatgacgac aatggagggc gatgtgcttt
    53941 acctctgcct tgaggacacg ttctgccgca tacaggacag gctgttccgt ttgacggacg
    54001 aagcgagcgg gcggcttcat tttgccgtgg caagctgcaa gctgtcagac ggtcttatcg
    54061 tgcagcttga ggattatctg aaagactatc cagacagcag gctcattgtc attgatacct
    54121 tgcagaaagt ccgtacagct tcaaaagaca acgcctatgc aagcgactat ggggacatct
    54181 ccctaatcaa agattttgcc gacaggcact ctctggcggt cattgtcgta caccacatcc
    54241 gaaagcagaa tgacagcgat gtgttcaaca aggtgtctgg gacgacaggc ttaacgggga
    54301 gtgcggacgc tacctttgtt ctggaaaagg agaaacgggc atctgacacc gccaagctgt
    54361 atgtaacggg cagggacacg ccctatcagg aatacacgct ccgtttccgt gattgcagtt
    54421 gggaacttgt ggagaggaaa acgcaggagc agcttgcgaa agaaacgata ccagatgtcc
    54481 tttttcggtt ggtggatttt atgagggata aggaagaatg ggcaggcacg gcaacggagc
    54541 tgttagccgc tatgggggaa acggaaacca tacccacggt gattacgaaa tggctgaatg
    54601 aatatcacac cacattttta agcgagaacc gtatctgcta ccagtacagc cgcaggaaag
    54661 acggcaggca gattgccctt gcaaggaggg cgggtgacag cggtgacggc ggtgacagcg
    54721 atattgggat accccctgtt actgtcattg acgcttaaag ccatgtaaag ttggcggctc
    54781 tgccctgcgg gtgacagcgg tgacggcagt gacagtgatt ttaggatacc ctgccgctgt
    54841 catccctacg ggagaacacc ccgcaaacgc aaaatgcagg cgtgggattc acccgccctt
    54901 ttcgggcgtg taaaatcacc cctgcggtca gaaaaatctc tccgattttt ccgactgcgt
    54961 ttacagggtg taacacacta cactttgccc tgcaaagagc cgtgtgccag acgttccctc
    55021 tggactccct tatggcaggt cttacgccct gctatcctac gccttgcggc ttcggataaa
    55081 agaacggcgg catgacggtt acagctcttg cgagggagta tcccaaaaac accgtcacca
    55141 tcgtcatgac cgtcatgcag gggataaggg tgacagttac ggcaatcggc agggggcatc
    55201 ccaaaactgc cgtcaccacc gtcaccgctg tcacccaaag ggcagttacc cgccgaagaa
    55261 aggaagatga tggattatgc cctatgcaat cctgcgtttc cagaaacgca aagcgggcgg
    55321 cgttgcggct tgcgaacgcc acaacgagcg gaagaaagaa gcctacaaaa gcaacccaga
    55381 tatcgacatg gaacgctcta aaaataacta tcatcttgtc gcaccgccaa agtacaccta
    55441 taagaaagag attaaccgaa aggtagccga agcagggtgc aggacaagga aagacagcgt
    55501 gatgatggtg gaaacgctca tcacggcttc accagaattt atgaaccagt taccgcccga
    55561 agaacagaaa gcgtatttta cgatggctct ggatttcatt tcggagcgtg tcggggagca
    55621 gaatatcctc tccgcagtcg tccacatgga cgagagaacg ccccatatgc acctctgctt
    55681 tgtgccgatt acaccagaca ataagctgtc cgccaaaaca attttaggca atcaaaagag
    55741 cctgtcggag tggcagaccg cctaccatga gcggatgtcc tcacgttgga atcagcttga
    55801 gagaggtcaa tcttcgatgg aaaccaagcg gaaacacgtc cccacatggc tctataagtt
    55861 gggcggcagg cttgataagc agtatgggga aatcgtgtct gccctgtccg acatcaacgc
    55921 ctttaacgca gggaaaaagc gtgacaaggc tctggaactg gttgccgcat ggctgcccga
    55981 agtggagaaa ttctctaagg aaatcggcag acagcaggcg tatatcgaca gcttaaagga
    56041 gcaaatcggg caggaagccg actatgcagg gcgtatgcgt gatgaaaagt acgagcagga
    56101 attaaaggtg cagaaagcca accagaggat atttgaattg cagagaacca acgagcagat
    56161 ggggcggctg ctctcaaaga taccgccaga agtgttggaa gaattacaga gaacaggcag
    56221 aaacaaatca agagaaaggt aaaaagtgaa tgaagaaaca ggattttaag gtgttaaaga
    56281 ccaaagattt atacccgttt cccgacaatc cgtttcatgt tgtggaggat gaaacgctgt
    56341 cagagttagc ggaaagcatc aaggaatttg gcattgtcac gccgataatc acacgcccga
    56401 aagaggacgg gaacggttat gaagtgattg cggggcagcg gcgtgtccgt gcgtccgaac
    56461 ttgcaggtat aaataccgtg cctgcatttg tcctgccctt agaccgtgac cgagccatca
    56521 tcacccttgt agacagtaat ttacagcgtg aaaatatcct gccatcggag cgggcgtttg
    56581 cctacaggat gaaatccgaa gccatgaagc ggcagggttt ccgcacagac ttaacctcgt
    56641 cacaagttgt gacgaagttg cggacggacg acaaagtggc acagggcttc ggcgtgggca
    56701 ggatgaccgt ccagcgtttt atccgcctga cggaactgat accgccgatt ttgcagatgg
    56761 tggacgaggg gaaaatcgcc ctcacacctg cggtggaact gtcctttttg aagaaagacg
    56821 agcaggaaaa tctctttgcc acgatggaga gcgaagaagc aacgccctca ctctcacagg
    56881 cacagcggat gaaaagcatg agccagagcg ggcagcttga catggatatg atattttcca
    56941 ttatgacgga ggaaaaggga aaccagaaag aaaccttgaa aatcaacaca agcaaactga
    57001 aaaaatactt tccgaaggac acaacgccga agcagatgga ggaaaccatc atccgacttc
    57061 tggaacgtga gttgcagaga aaacggagca gggacagccg ctaaccttct tttttcggga
    57121 agtaaatgca ggaatttgga acagaagaaa gacagaattt taaggagaat gaggtaagga
    57181 atgaaagaaa tccagtatga gattgtaaag gaaatcgccg tattgtctgc gagtgacagc
    57241 ggctacacaa aggaaatcaa tcttatttca tggaacggga gagagccgaa atatgacatc
    57301 cgcagctttt ccccgaaccg tgagaagtgc ggaaagggta tcacgttgaa cgctgatgaa
    57361 gcagcggcac tccttgaagc attacagaaa gaagtaaaca gcggggattg atggtatctg
    57421 attgacaggg cggggcgttt ccagacgttc tcctgccctg tctggaaagg aagatttaag
    57481 tatgggcgag gataggaaaa caactggaag aagaaagcgt attgtgccaa aagggcaagt
    57541 acaaatgatt atcagtcgag aatatatcgg cacacagacc gttgcagaag cgtttgtccc
    57601 gattatctcc gaggatattc ggaagaagat tgccgagggc gacaccttcg acaatgaggg
    57661 gctgtccgct tagaatgtac gcaatggaac atgaaaacgt taggacagat atggaggttt
    57721 tacagtatgg caggaataag aacggagaac aagatttatg aagtcggcat ttactgccgc
    57781 ttatcaaagg acgatggcac ggataacgag agtgcgagca ttgcgacaca aaaatccatc
    57841 ctcacggatt atgtgaaaag gcagggatgg catttagcaa aaacgtatgt tgacgatggc
    57901 tactctggaa caaatttcca aagaccaagt ttccagaata tgattaagga cattgaaaac
    57961 gggctgataa actgcgtgat tacaaaagat ttatcccgtc tggggaggaa ctatcttgat
    58021 tgtgggttat atctggaagt ctttttccca gagcataacg tgaggtatat agcggtcaat
    58081 gacggcgtgg acaccttaaa caaatccgct atggacatca cgcctttccg caacatccta
    58141 aacgaaatgt attctgccga tgtgtcagtc aagataaaat cggcgtaccg tgcgaggttt
    58201 cagcagggga aattcatggg aacaacagcc ccttatggct atgtcaaaga ccctgccgac
    58261 cacaaccatc tgctgataga tgacaaagtt gcccatgtgg taagggagat attcgacctt
    58321 gcgttagcgg gcaacggcat cgccaaaatc cgcaagcaca tcaacaaaca gcatatccta
    58381 cgccctgccg cttatgcggt ggagcaaggg gcaacaggct atgagcggta ctttgaggat
    58441 aacgaggaaa accgttatat ttggagcgag aacagcgtga ggggcatttt aagaagcccg
    58501 atatatgcgg gcaaccttgc aggctacaag cggattgcag ccaacatgaa aagcaagaaa
    58561 cgcccctcta agctgcccga agaatgggaa gtgataccag acacccatga ggggatagtc
    58621 acgcaggagg aatttgacac cgtacagcag cttatgacga gccgcaggcg ggagcagaac
    58681 gcagggggat ttgagaatat cttttcgggc gttatcaagt gtgcggactg cggctatgcg
    58741 atgcgggcgg cgagtgccaa caggaggaaa cgccccgaca tcatcgactg cgtacaatac
    58801 acctgcaata attatggcag atatggcaat gttatgtgta ccgcacacag cattgaagcg
    58861 agggacttat tcaatgccgt ccttgccgac atcaaccgct ttgcggatat ggcggtcaat
    58921 gatgaaaagg cggtcagagc catagaaagg cggctcacgg aaacagacca gagcagggcg
    58981 aaatcattag agaaagaaaa gaagaagctg aacaaacgcc ttgcggagct tgacaggctg
    59041 ttttcctctc tctatgagga caaggtaatg gagcgtatca cggagcggaa ttttgagatg
    59101 atgtcgggga aataccagaa agagcagctt gaaattgaag cacggctgaa agaggtaacg
    59161 gaaacgctca atgacagcta tgagaaatcg cagggcgtga gggactttct atccctaatc
    59221 cgcaactatc aggggctgaa agagttggac gcaaccatca taaacgcact catagacaag
    59281 atacttgttt cggaacgtga gaaacttgcg gacgggacag taaggcagga aatcaagatt
    59341 tactataaat tcatcggctt tgtcggtgaa ttacatatca cacccacaaa gcggtggacg
    59401 gcgttaaagc ctaaaaattg tacggtgtgc ggtgttgaat acgtccccag ctctggaata
    59461 tcaaagtatt gtcccgcttg tgctaagagg atacagaggg agaaatcaaa cgagagcaaa
    59521 cgcaggagta gggagaggaa ccgaagggca tgtattgaac tgtccgcaaa aaatgaccga
    59581 ctgatattgt ccagcgaaat ggacgactga ttcgacaagg aaacatgcat caggaggtag
    59641 atatttatca ctatattact aaagggagct ttgacaatta cctctggcag acgcaggaga
    59701 ataagctcaa gtatatcacc cagattatga cttcaaaaga tcctgtgaga tcagctgaag
    59761 acattgatga acaaaccatg actgcttctg aatttaaggc cttggccact ggtaatccct
    59821 accttaagct caaaatggaa ttggaaaatg aactaacggt attagacaat caaaaacgag
    59881 cttttaatcg ctcaaaagat gaatatcgcc acacaatttc ctattgcgag aagcacctcc
    59941 ctatcatgga aaaacggttg agtcaatatg ataaggatat tgcccaatct ttggcaacaa
    60001 agtcgcaaga ttttgtcatg cgatttgata atcaagtaat ggataatcgt gctgaagctg
    60061 gggactatct gcgaaaactc attacctata accgctcaga gaccaaggaa gtcaaaacac
    60121 ttgccagctt tagaggattt gatttaaaaa tgactacacg aggtcctagt gagcccttgc
    60181 cagaaaccat tcctttaatg attgcaggtg ataaccagta tactgtcgcc cttgatttga
    60241 aatcagacgt gggaaccatt caacggatta gtaatgccat tgaccatatt atagatgacc
    60301 aagaaaagac gcaagagctg gtaaaggatt taaaagataa gctacgagta gccaaagtag
    60361 aagttgaaaa agtctttcca aaggaagagg actatcagct tgtaaaggct aagtatgatg
    60421 ttttggctcc cttggttgaa aaagaagcag agattgaaga gatagatgca gctttggcca
    60481 agtttagtga agatacaaca ccccaaaaga agcaacaaat agcactcgag atataagaaa
    60541 aaaggctgac aaataatttt gaacgcggta aaatgaagtc aagaaatcac aaaaggagaa
    60601 cagaccatga ccaaaacagt agaagaaatg cgttatcaac tagaagaatg gttgtcacaa
    60661 ggctttacta gttcagaaga tagggcaaac taccaaactt taaaggaaca gtatgaagat
    60721 gagacctttg attacagttt ttcaaggcgt gaaattactg gacagctgga actcatcatc
    60781 acaagtcgtg agaatgattt tccaaactta gatgaggtga cgaaggcaga ataccttgat
    60841 ttggttgccc aacttgatga tttagacaag agacaggctg actattatcg caagcaatta
    60901 gcctagaaag aggtgtaaga tgttggagca aattctacaa agccttttga ttatcgcagc
    60961 aataggactg atgttgtttg tcctttatcg gattgtgaaa gtttcaggtg ctttatttct
    61021 tatcggactc atcagtggat tagtctttat tgaaatatat ggaatttacc tattcttcac
    61081 agaaagacac ctatattcag aagatttagc agctaatggc atatggagtt tcactggatt
    61141 ttatattgca ttgaatttat ttcttgctat tagcttaagt atgaggtggt ggagaaatag
    61201 aatcgtttaa catggggatt acctactggt agtccttttt cttgtgataa aatctccaag
    61261 ctaatcttga tatgatgaag ctatcactaa tagaggagta agccatgtta aataaagtga
    61321 aagctcgatt tctgattgga gtaggaggtt taatcgcagt cagttttatg gtcatgattg
    61381 gctacacgat tggctcacaa accatttcaa agcaaacaga gaaccaaata cgagcagaag
    61441 ccaataaact tgtttcaaag aaaaatcagg aggaaagagc gacagtccta tcagatgagc
    61501 ttgtcaaaga atttctcact caatattata caaaggtcca gctaggagaa aataacagtc
    61561 gtatcaaaac ctacatgacg gattcggcat tttcagaaga ggaagcaaat cagaataaag
    61621 cgatcaatca agtttataaa gactatatgc ttgactatcg atttgaatca gccagtatct
    61681 atgtcaatac agaaagcaat gttgcacttg cggaagtttc ctatcaagtg acttatgttt
    61741 ctgatttgag tgagcaacaa cagcgaacaa ctcagacaga aaccaagaca gttatattat
    61801 cctactccaa agtttccgac aagctcctgg ttaatcagtt gactatttgg aatgggaaac
    61861 tggaggacat gaaagaagcc acaaatggtg ctaattccag cataccaacg atccaaggaa
    61921 ctacaacaag tgagaacaac tagcaggaga cagtcttctg ctttttttga tagggaggaa
    61981 agatgacacg aattaaagca gtaaaacaaa aggccatttt agatgttgct gagagtctgg
    62041 gctgttcctt cagacgttta tcaggacaca tttatgaaca cccagaccat gattcctttc
    62101 ggatttttgc cgataccaat actttcaaat ggttttcaag agatatacaa ggagatgtaa
    62161 ttgactttgt tcaattagtg acaggtgttt ctttcaaaga ggctgtatcc tatcttgaaa
    62221 ctggggattt tgaacaagtt aagttgatag aagaaactta tcaaccattt caatactatt
    62281 tgcatgaaga accctttcag caagcacgta cttacttgca agtcgtccgt ggcctaagtg
    62341 atcagactat taataccttt ggcagacaag gattgcttgc tcacgctact tatcaatcgg
    62401 agcccgtatt agtgttaaaa agctatgacc acaacgggac cttacaggcc gcaagccttc
    62461 aaggtctcgt caaaaatgaa gaaaaacacg atcgaggtta tctcaaaaaa atcatgaaag
    62521 gatctcatgg ctatgtcggt attagtttcg atattgggaa tcctaagcga ctcatttttt
    62581 gtgaatcagt tatcgatatg atgagttatt atcagcttta ccaaaagcaa ttatccgatg
    62641 ttcgcctgat ttcaatggaa ggtttaaaac tttctgtgat tgcttatcag accttgcgtc
    62701 tagcagcaga ggaacagggg aaattggcat ttctagatac agtaaatcca agcagactta
    62761 gtcattatct tcaggcaata caagagacga caaccttctt ccaaactcat tcaaatgcat
    62821 taacattggc tgttgataac gatgaggcag gaagagaatt ttgtcagaaa ctgtcagata
    62881 aaggacttcc gctttctcaa gatttaccac cattgcaagg acttgaaaca aagttagatt
    62941 ggaatgatat tgtgaaacga caaaaggaat tatctttaag agatcttatc cagtcagccc
    63001 aaacaaaagt cattagagat catcctccac ctaaacgaga gcacactttt gatgtgataa
    63061 caaaatcaag tccgctatca taatctagaa tgtgacaagg aggacaccct atgagtgtga
    63121 ttgaacgtct ggctgaaaaa gtagctagac aagaagaaaa ggtctcacgt gagacggaga
    63181 aattggaaca ctatcgtgac caactacaaa cagctatgta cagtaccttt atcaaacggc
    63241 aacaatctag tcagttgtca tttcatgaag cactagagca agcctttggt aaagaaacca
    63301 cactacaccc aaattacaga aatgaggata cagaatgagt aaaacatgga attttgatca
    63361 gccactagat gatgtgaaac caacatcatc ccatgaagaa cgagcaaaaa ttgcagcact
    63421 tttccataaa caagatgaaa aaccaattga agaagtagac tatgtggctg cttttgaaca
    63481 gcaacaaaag gagtcagaat ctaaagatgt acagacgccc gaatcaaaag ttaaacaaag
    63541 ccagccgaag aaagtaaata tcacaagcga ctataaacaa cacttggcag acactattgc
    63601 acaaaacaac aaggatattt cagcctgtca aaagcaaatt gaagaacttc atcaattgat
    63661 tgatgaaaag aaaatccaaa ataaaaagtt gcaggctatt tcagtagcca ttgatgattt
    63721 ataagtcagg tagtcctatg cgggctacct gacttataat tgaaggagaa gataatgaaa
    63781 ttttttcaaa agaaaaaaca gaaccaggat caatttaaac gactgattca tcgactatca
    63841 gagatgtcag atagtgaact aaccaaagta gagcaactcc tagatgtggt ctttgattca
    63901 agtttcaagt ctagtgagag agtggaaata tctagggaca ttataaccga tgaggaacaa
    63961 attcttgata agtcaatcca agaagcgaaa aataaactga atactgagca gttggagaag
    64021 cgaatagagg agtttaagca atcgaagaaa cctaaaaacg gataatgcac caaatatggt
    64081 gcgttatccg ttttttatgg tataatgaaa gcaaatatga ggaggtcttt atctatgatc
    64141 ggagacaata taaaatcact acgccgaaca cacgatttaa cacagccaga atttgcgaaa
    64201 atggttggga tttcacgcaa tagcctgagt cgctacgaaa atggtaccag cactgtttca
    64261 actgaactca tcgaccgtat ttgtcagaaa tttaacgttt cttatgtcga tattgtagga
    64321 gaggacaaga tgttaacacc tgtcgaagat taccatctga ctttaaaagt agaagtgata
    64381 aaagaacgtg gagcagccat tttatcaaag ctttatagat accaggatag tcaagatatt
    64441 gtttttgatg atgaatctaa tccttggatt ttgatgagtg atgacttggc cgaattgatc
    64501 aatacgaaaa tttaccttgt cgctactttc gatgaaatag agcgttacaa tggctattta
    64561 gatggtatag agcggatgtt agacatggtg catcatcggg tggtggctta atgagactgg
    64621 aagaatttag tgaggctgag tttcagaagg ccttgcagcg aaccattcgg gccttaaccc
    64681 gaggaaagac gattccagat caaccgaaag ctatcttgct tggtggacaa agcggagcag
    64741 gtaagacgac tattcatcgt atcaagcaaa aggaatttca aggtaatatc attattatcg
    64801 atggtgacag ctaccgttct cagcatccca attacttagc cctgcaagaa aagtatggca
    64861 aggatagcgt ggactatacc aagggatttg caggaaaaat ggtagaacag ttggttgatg
    64921 aactcagtac gcaaggctac catttgctga ttgagggaac cttgcgaact actcaagttc
    64981 ctcgtcagac tgctcaatta ttatcttcta aaggttacca agtttcctta gcagtgattg
    65041 gtaccaaacc agagctttct tatctcagca ccttgattcg ttacgaagag ctttatgcca
    65101 tcgatccgac tcaggctaga gcaacaccaa aagaacacca cgatgggatt gttgagaact
    65161 tggttgataa cttgagggag ttagaaagtg aaaatctctt tgaccagatt caaatttatc
    65221 aaagagatag aacttgtatc tatgattctg aaactgctga aggttccgca gcagaagtgc
    65281 tacaagaatg cctatttgga aaatggagca aggtagagga ggagatggtg aaggagagtg
    65341 aactcctact taaggaatta cttgagaaaa ataaataatt gatt
//
LOCUS       DAZQCJ010000003        62174 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000003 DAZQCJ010000000
VERSION     DAZQCJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 62174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62174
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.003"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            5..2674
                     /gene="acnA"
                     /locus_tag="ACHU66_000180"
     CDS             5..2674
                     /gene="acnA"
                     /locus_tag="ACHU66_000180"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR3388496.1"
                     /translation="MEKSHRKRLIHKGEEYSYYALDSISMEEKVDIHSLPYTIRILLE
                     SLLRKEDGVDVTKNHIMELLHYQAASPKGEIPFKPSRVILQDFTGVPVVVDLASMRDA
                     VVKAGGNPELINPEIPVDLVIDHSVQVDFFGTEDALEKNIALEFERNNERYEFLKWAE
                     NSFENYRAVPPATGIIHQVNIEFLSDVIINKDGLLYPDSMFGTDSHTTMINGIGVLGW
                     GVGGIEAEAAMLGEASYFPVPEVIGVRLAGQLPKVVTATDLALKVTQLLRQENVVGKF
                     VEFFGPGLSSLALADRATVSNMAPEYGATCGYFPIDGETLHYMRLTNRSEEHVELTEA
                     YTKANYLFYDAERFPSYSKVLELDLSTVVPSISGPKRPQDLIELTDAKAEFQASLIRE
                     VGVRGFGLEEAELDKTATVKYVEGDEQIQTGHVAIAAITSCTNTSNPYVLLAAGLLAK
                     NAVEKGLAVSKTVKTSLAPGSKVVTGYLKKSGLQTYLDALGFNLVGYGCTTCIGNSGD
                     LRPEVAEAIKEEDLLVSAVLSGNRNFEGRINPLVKANFLASPPLVVAYAIVGNMNVDL
                     TRDPLGYDEKQQAVYLADIMPSREEVDDYIERYVTRDLYKEEYQQVFTDSQAWNAIET
                     KTDKNYNWNSSSTYIQNPPYFDNMQVDLSIKPLENLSVLAKFGDSVTTDHISPAGNIA
                     RLSPAARYLEENGIVYKDFNSYGSRRGNHEVMMRGTFANIRIKNELAAGKIGGWTRVG
                     DEILPIYDAAMKYKAAGIGSIVIAGKDYGMGSSRDWAAKGSSLLGVKAVLAESFERIH
                     RSNLVMMGVLPLQFLEGQSAESLGLTGHESYTIDLPEDVGVGQIVTVHAQIDDVTKEF
                     QALVRFDAEADIRYYRHGGILPMVVRKKLGGKV"
     gene            2671..3789
                     /locus_tag="ACHU66_000181"
     CDS             2671..3789
                     /locus_tag="ACHU66_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR3388497.1"
                     /translation="MTETNGLKDAIACDTRISAIENDRLSYAGYDIAELMENNASFEE
                     VIYLLWNLHLPTQIELNYFEKSLRKEYAISDAVEQCILIQSRRHLHPMSVLRSTVSLL
                     GVYNVHAEENSLEALYEQSIQIMGKVPTIIATFARLRRGLVPIEPRSDLGFAANFLYM
                     LNGEEPSDLQIKAFNKALVLHADHELNASTFAARVTASTLTDLYSCVTTAIGTLKGSL
                     HGGANERVFDMLLAIRESGDAERYLQKKLDSKEKIMGFGHRVYKTVDPRQEYLKEMAR
                     DLTFGTDDEAFYRLSEEVESFIKNKKGLIPNVDFYSATVYHVLGIDSDIFTLIFAMSR
                     MAGWIAHVQEQRKNNKLIRPRSSYLGGRNLVYIPIGKR"
     gene            3792..4973
                     /gene="icd"
                     /locus_tag="ACHU66_000182"
     CDS             3792..4973
                     /gene="icd"
                     /locus_tag="ACHU66_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR3388498.1"
                     /translation="MAEKILMREGRLIVPDCPVIPYIEGDGIGRDIWSVSQKVIDAAV
                     KKAYAGQRSIQWREVLAGEKAYELTGTSLPNETLEIIREHLVAIKGPLGTPIGKGHRS
                     LNVALRQELDLYACVRPIRYFKGVPSPLKEPEKTDITIFRENTEDIYAGIEWEAGSDE
                     VAKVISFLQEEMGVVNIRFPESSSIGIKPISEEGSKRLIRSAIEYALEHQLKIVTLVH
                     KGNIQKFTEGGFRKWGYELAKEEFATELAEGTLVVNDIIADNFLQQILLAPENFQVVA
                     LTNLNGDYASDALAAQVGGIGISPGANINYQTGHAIFEATHGTAPDIAGKDLANPCSL
                     LLSACMMLEYIGWKEAATLIIQALEDAFINKNVTLDFAKILDVAHQSTSQFSEVLIDS
                     I"
     gene            5129..5392
                     /locus_tag="ACHU66_000183"
     CDS             5129..5392
                     /locus_tag="ACHU66_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFR3388499.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLNYKEKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAITETMEKEGLA"
     gene            5397..7130
                     /gene="ptsP"
                     /locus_tag="ACHU66_000184"
     CDS             5397..7130
                     /gene="ptsP"
                     /locus_tag="ACHU66_000184"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR3388500.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVEDTNAEEARL
                     DAALEASQNELSVIRENAVASLGEEAAAVFDAHLMVLADPEMIGQIKETIRAKKTNAE
                     TGLKEVTDMFIAIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVRLPNPATIDEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     VLAVNGITGDVVINPSEEVIAEFKVAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPHEMNPFLGFRALRISISETGNQMFRTQLRALLRSSVHGKLRI
                     MFPMVALLTEFRAAKAILEEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYAHRALTECATAEEV
                     LELQKEYVDFD"
     gene            complement(7212..8699)
                     /locus_tag="ACHU66_000185"
     CDS             complement(7212..8699)
                     /locus_tag="ACHU66_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFR3388501.1"
                     /translation="MKKIAFDSTKYLNLQRDHILERIAQFEGKLYMEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKDQVEIVIAINASNIEHSKARGDLGISYDQEVFRLIDTF
                     NDIDIYVGSVVITQYRNQPAADAFRKQLEKHGIKSYLHYPIKGYPSDIDHIISPEGMG
                     KNDYIETSRNLVVVTAPGPGSGKLATCISQLYHDQLHGVTSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDLNMIDPFHLQTYGKTAVNYNRDIEVFPVLNRTFERILNKSPYA
                     SPTDMGVNMVGYSIVDEEAAIEASKQEIIRRYYQTLVDFKAERVSEQAVKKIELLMNE
                     VGVTPADRKVVIAAREKAELTTSPALAIQLPTGEIVTGKTSDLLKPTATVLLNAIKQI
                     ANIDDETLLIEPNYIRPIQELKADYLDKTNTRLDASEILNALAITAQDSPLAARAMKE
                     LGQLNGSEAHSTVILSDEDKSVLRKLGINLTFDPIYQHNKFYQKN"
     gene            8971..10239
                     /locus_tag="ACHU66_000186"
     CDS             8971..10239
                     /locus_tag="ACHU66_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR3388502.1"
                     /translation="MKKLIHNKLFLSSFVADLISNFGDTLYYLALMNHVLLLADTKFA
                     LAMITASETLPILAGLFIGIWADKTKNKLDTILATLVVRVGLYAFVGFLMGFAPALWV
                     VAVVCVINFLSDLAGQYENGLYMPVSLRVVAAEDRETAMAFKMTVKSLLQIAFQASGA
                     ILIGFMSYQNLAFFNAGTFLVSLAIMATLRPALAKLLKENPIQEAEQTETEAGLIKGM
                     RNSLKESYQAVQNILVLKASIVTIASLNAIFTALSPLVILNMKEFSDFVIVNAGTTVA
                     LISIFFSVGSILGSSIGMALFKNVSLINLLKFSTLMPVLIFSGFFLHNIYVVLTVIFV
                     TAMTLGVFNPKMNALVLNELPEDKLATVGGGIDAFCQIGMVAGQALVALMVTILSVTS
                     ISLIFLLLSVGLVSYTILVGCKPKNEVAQV"
     gene            10251..11291
                     /locus_tag="ACHU66_000187"
     CDS             10251..11291
                     /locus_tag="ACHU66_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFR3388503.1"
                     /translation="MKLDYRDYRSEIVEKLFIPLIVKEREEPFEADLSQKEKDILKDA
                     LDIRNEVENKLADFRQEVNEVFVWGHIFTILHTLYFYLLDQGQDPKTVEEACQLILTL
                     SQEEVEDAMRTMLASENDGHREKDLSLMELLEKTDKKPAEKWYWSLALRNPLETVERS
                     VDLLNKLLPIYQPYFEGARAEREAFARDFDIEQLYRESKQLAMTSLDSLGVETAQFFV
                     LSPWNYWFAYYGNEQFDYMKVALLASCRIDQIMLSNDELDLDDLTTALKVISDSTRYQ
                     VLVELTKPHAKSKDIAERMAITGAAVSFHTQKLINGDLLLFNTKNSDVKYSVNRELLQ
                     EVIAKLTEDFDL"
     gene            complement(11428..12417)
                     /locus_tag="ACHU66_000188"
     CDS             complement(11428..12417)
                     /locus_tag="ACHU66_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR3388504.1"
                     /translation="MQNKTKQLNTSLLSLIQFIGAILVVALHCRRLFEADQLHFIQKS
                     IFSRMVVPYFMVTASFFLRRNYPSLSKQYLIRYSKQYLTWSILYLPFYLIYLTLQEIP
                     FLYYPLALLVGLTYTGTSYQLWYMPAFFLGLILVHLSLEKWGWRITACLACLLYLLGS
                     VETYSAYLAESIFLTAFDNYKTFFFTSRNGLFYAPIFVLIGFYLANKLNHPIFQHRQK
                     TKLLVCTALLAFEATVIYLNQGYDKNFLFSLIPFTAYLVAWTLTTDLFRKKKFQFLKE
                     YASYYYFIHVIPVEISFFFLETSSLTKIQQGWLVFLITIITCQLLSWLIINTQ"
     gene            12606..13292
                     /gene="rnc"
                     /locus_tag="ACHU66_000189"
     CDS             12606..13292
                     /gene="rnc"
                     /locus_tag="ACHU66_000189"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR3388505.1"
                     /translation="MKDLHAKLLADFGIDFSDLNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLMISKYLYQKYPDRPEGEMSKLRSTFVREESLAGFSRACGFDRFLRL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKGEKTVEEFIHKVMIPRLEKGNFERVTD
                     YKTALQEILQVNGEIAISYQVIAESGPAHDKTFEVEVSADRRVIGRGRGRSKKLAEQA
                     AAKNAVEARG"
     gene            13295..16828
                     /gene="smc"
                     /locus_tag="ACHU66_000190"
     CDS             13295..16828
                     /gene="smc"
                     /locus_tag="ACHU66_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFR3388506.1"
                     /translation="MYLKSIEMQGFKSFADKTKVVFDRGVTAVVGPNGSGKSNITESL
                     RWALGESSAKSLRGGKMPDVIFSGTESRKALNYASVVVTLDNSTGFIANKQKEIKVER
                     HIYRSGDSEYLIDGQKVRLRDIHDLFMDTGLGRDSFSIISQGRVEAIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETESKLAQAQGNLDRLDDIIYELDNQVKPLEKQAQTAKKFLEL
                     DGQRKELYLDVLVAQLLLGKEKLSEKEAELESVKTELTSYYKQRSELEQENQNLKEKR
                     HRLSEQLEREQAVLLDLTKLISDLERKIEVHKLESSQNESSRQEAQARLENLLTRREQ
                     LAEQIEQKQETLAQLDSSLSSLKDDIAAVDKEISYFSEDPDQVLDHLREQYVALMQEE
                     AEASNSLTKIQQDIANQISLSESKSEDLARLQTEKQTAQEVLDKSRKSLEEADHVLRQ
                     LLESYQIKKSELDQTQTAYQAEQGRLFDLLDQLKGKQARQSSLEAILKNHSNFYAGVK
                     AVLQAAPSLGGIIGAVSEQLTFDTRYQTALEIALGGASQNVIVEDEATAKRAIAFLKE
                     KRQGRATFLPLTTIKPRQLSGQQVSLLESSEGFLGLASDLVTYQPNLDAIFQNLLGTI
                     AIFDSIDHANQAARATKFQVRMVTMDGAEIRPGGAFAGGSNRNNSTTFIKPELDALLG
                     EIAELSSQLQEQESLVAAKKTSLDQTREALETIKAEGEEARLNQQSARIHQEQAENRL
                     AQLSAQYDLQMSQVSPTILTELEEAAAKEEVNVQALNEKKLALDQQINQVRDNRDSIQ
                     ESLQKLQTQKGQLTLEQAELTSQLRFEQTDLRRLQEEKVVADKEISLLEDMIDQKLEA
                     LEDTSIEILEEQLQAASDKQNQTNQILIRLKFELEDIDGQFEDLEERLQQARTKNDDL
                     IRKQAKLEADCEQAGDKLRTLLGNLTEHFKLSFEAAQSQAKEVENLAVAEQSLKDLER
                     AIRALGPVNLDAIEQYDEVNNRLTFLNEQRTDILSARDLLLDTIHEMDDEVKERFKVT
                     FEAIRESFKQTFKQMFGGGSADLILTEGNLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKESQFIVVTHRKGT
                     MAAADAMYGVTMQESGVSKIVSVKLKDVEKL"
     gene            16825..17634
                     /locus_tag="ACHU66_000191"
     CDS             16825..17634
                     /locus_tag="ACHU66_000191"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFR3388507.1"
                     /translation="MINKIYASDMDGTFLREDHSFDKERFRRILNQFKEKGYLFVATS
                     GRSMQSLKLVFEDFVDEIGFVAENGSIVEYQGQTIFMDDPISPEIYLPIIAGIDAGPF
                     GSSRSMVLSGLENFYLLKNAEPQFLEAMTNYYAHFRLVDTFEEVREEIIKINAKFSPE
                     EMDDARRWLNDTFEGVTAMTTGFDNIDIIPNGSNKSVGLSHLCAHFGITRQDVVAFGD
                     NQNDLDMFDFAGLALATENAREEVKAQADWMIGHCNDGAVLAYLEEEVNGN"
     gene            17624..18436
                     /locus_tag="ACHU66_000192"
     CDS             17624..18436
                     /locus_tag="ACHU66_000192"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFR3388508.1"
                     /translation="MAIKLIALDLDGTLLTSDKRISEANKKALQAARERGVYVVLTTG
                     RPLQAIGTFLEELDLLGENQYSITFNGGLVQENTGRVLDKVGFTIEDVRTIRRVTNDL
                     DLPLDVLYGGDVYSLPSAHESLYLTCNPLLNKIVASDEEMPEDFVYNKAVSAVAADFL
                     DGQIPKIPAELHERFEIFKSRDILLEWSPKGVHKANGLAKLIGHLGIDQSEVMACGDE
                     GNDLSMIEWAGLGVAMANATDEIKSAANLVLPKNNDEDGIAWVIEHYVLNED"
     gene            18438..19712
                     /gene="ftsY"
                     /locus_tag="ACHU66_000193"
     CDS             18438..19712
                     /gene="ftsY"
                     /locus_tag="ACHU66_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR3388509.1"
                     /translation="MGLFDRLFGQKKQEEQVALVEEVEREQSASSEDTVTTEVESATQ
                     EMEKFQSEVAETELTAEQEAQKQRTLDMMAQYYAAKEAAAARVKEAQEQGFDPAIQTK
                     VKEEPVEEVAPVGQESEQDKYQRTLKKTRTGFGARLNEFFANFRSVDEEFFEELEEML
                     ILSDVGVQVASTLTEELRYEAKLQNAKKTDELRRVIIEKLVDIYEKDGQFSERINFQD
                     DLTVMLFVGVNGVGKTTSIGKLAYKYKQAGKKVMLVAADTFRAGAVAQLVEWGRRVDV
                     PVVTGPEKSDPASVVFDGVKRAVAEGVDILMIDTAGRLQNKENLMAELEKIGRIIKRT
                     LPDAPHETLLALDASTGQNALSQAKEFAKITPLTGLVLTKLDGTAKGGVVLAIRQELD
                     IPVKLIGFGEKIDDIGEFKSEEFMRGLLEGLV"
     gene            complement(19736..20293)
                     /locus_tag="ACHU66_000194"
     CDS             complement(19736..20293)
                     /locus_tag="ACHU66_000194"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR3388510.1"
                     /translation="MQHKGTQILETERLILRPFQASDVESVFQNWTSDEKVTTYLTWP
                     THQTLQDSEDYVQFCLQSYSQEKTYRWVIELKENQQPIGDISVVSLDERVQAAELGWV
                     LGSKWWGQSYMAEALEAVNHYLLEEVGCLRITAVHDSENRPSGRVMEKVGMTYEGTLR
                     QAARNNRGIVDIAIYSLLHTDRKSR"
     gene            complement(20295..20969)
                     /locus_tag="ACHU66_000195"
     CDS             complement(20295..20969)
                     /locus_tag="ACHU66_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFR3388511.1"
                     /translation="MTELNTILTQLQAASHAGTLKRYEKIGETKPYYGVPMGAISGIA
                     KAYKNRLDLFAPLWQTGVLEAQYLAIQIAKNKPDQLPQADLENCLNEQISVNVLDKLA
                     SIILSKRKDSRVWEEDLLAKDQAIFQRLGWFLRAKYFAGKTAKNQEIEETLDHIRQHL
                     QTADPLIQWTMNQCLVEIAVAYPDYLEQGLVIGQELAVYSDMKVPKGCTSAYAPDWIE
                     ALLRRK"
     gene            complement(21111..22598)
                     /gene="zwf"
                     /locus_tag="ACHU66_000196"
     CDS             complement(21111..22598)
                     /gene="zwf"
                     /locus_tag="ACHU66_000196"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR3388512.1"
                     /translation="MSSHVLFTIFGASGDLAKRKLYPSLFRLYKAGHIKENFAVIGTA
                     RRPWTKEFFEQTVIESLGDLPDTPRQAHEFASHFYYQSHDVNDTEHYVALRKLQDDLC
                     EKYNTQHNKVFFLSMAPEFFGTIAKHLKSEQIVDGQGFERLIIEKPFGTSLETASKLN
                     DELAAAFNEDQIYRIDHYLGKEMVQNIFAVRFANIIFEHIWNRDYIDNVQITFAEAIG
                     VEDRGGYYDHSGALKDMVQNHALQVLSLLAMDKPASFKEEDVRAEKIKVFQHLRQPSD
                     EDLKRNFIRGQYAAGSIDGKDYVSYLDEPNIAEGSQTETFAGGVFFVDTDRFRDVPFF
                     FRTGKRLTEKGTRVTITFKHAEDIFGQPSEANVLTIFIQPTEGFMLSINGKEVGSHFA
                     LTPAKLNFRHNATALGNSPEAYEKLFFDVLNGDSTNFSHWEEVKASWSLIDRIVDLWA
                     SNQVPLHTYPAGTMGPEATFDLLESYGCKWVWTPDVWYRERGLLK"
     gene            complement(22677..23492)
                     /locus_tag="ACHU66_000197"
     CDS             complement(22677..23492)
                     /locus_tag="ACHU66_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFR3388513.1"
                     /translation="MIRFLILAGYFEMTMYLYISGKLDQYINLHYSYLAYLSMVLSFI
                     LAIVQLYVWVKEIRVHSHLKSKIAKLSSVGLLLIPLAIAWLFPTVSLDSTTVAAKGYH
                     FPLAAENDTNTQNQEGTTVQYLKPDTSIYFTKSAYQNQMREIADRYLAEETIIVTNEN
                     YMEVMEAIYDYPTEFSGKTIEMIGFVYNDPDNSQQFFLFRFGIIHCIADSGVYGLLST
                     GATTEFPNNTWVKARGTIKVSYHTSLKQNLPTLELQSMVQIEQPDSPYVYRVF"
     gene            complement(23489..24394)
                     /locus_tag="ACHU66_000198"
     CDS             complement(23489..24394)
                     /locus_tag="ACHU66_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR3388514.1"
                     /translation="MFGLDQLPTSVLQAGAIFLSIMIEALPFVLIGAIISGFIDVFIT
                     PDKVRKLLPKNKFLAILFGTFIGFIFPSCECGIVPIVNRLLEKKVPSYTAVPFLVTAP
                     VINPIVLFATFTAFGNSLLFAFYRALGAIIVSLVLGIILGFFVKGPILKGSKFDHHQH
                     GYSDKTSWQKVGFALIHAIDEFFDTGRYLVFGCLFASLVQVYIPTAVLTTIGHSPLTA
                     ILLMMLLAFLLSLCSEADAFIGASLLSSFGFAPVMAFLVIGPMVDVKNLLMMKHYFKG
                     KFIVGFIITITLVILGYSLILGGVV"
     gene            complement(24396..24542)
                     /locus_tag="ACHU66_000199"
     CDS             complement(24396..24542)
                     /locus_tag="ACHU66_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFR3388515.1"
                     /translation="MAVTYKRQDDLEKMLEEFASFEKLEEIEYPDPKSKENSEKNNLN
                     QDKK"
     gene            24665..26623
                     /gene="ligA"
                     /locus_tag="ACHU66_000200"
     CDS             24665..26623
                     /gene="ligA"
                     /locus_tag="ACHU66_000200"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR3388516.1"
                     /translation="MKTRISELVSVLNQYAKEYYQLDQPSVSDAEYDTLYRELVELET
                     AHPELILPDSPTHRVGGKMLDGFEKYSHVYPLFSLQDAFSREELEAFDQRVRKEFPQA
                     TYICELKIDGLSISLTYEAGNLVVGATRGDGSVGENITENLKRVADVPLTLPEAVDIT
                     VRGECYMPKASFDRVNKQRQEAGEAEFANPRNAAAGTLRQLDTGVVAQRGLATFLYQE
                     ASPSEATSQSQVLEKLDALGFVTNHEYCLAESIDDTWDFIEKIAERRDDLPYEIDGVV
                     IKVNDLAIQEELGFTVKAPRWAVAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLSPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLKVVDKYRKEQEIMPIP
                     SHCPSCQSDLQHYEDEVALRCINPICPSQLMSKLEHFASRDAMNIAGLGSSIVEKLFG
                     AGLVHDVADIYKLTVDDLLTLEGFKEKSADKLYQAIQTSKSNSAECLLFGLGIRHVGS
                     KASKILVEKFGDLETLAFADQEAIASLEGLGQVIAKSLTTFFASEGAQQLLAELKEAK
                     VNLTYLGQVVDENAALSGMTVVLTGKLERMKRNEAKAKLEALGANVAGSVSKKTNLVV
                     AGTDAGSKLTKAQELGIEIKDEAWLESL"
     gene            26634..27677
                     /locus_tag="ACHU66_000201"
     CDS             26634..27677
                     /locus_tag="ACHU66_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HFR3388517.1"
                     /translation="MEERKRARLIYNPTSGQEIMKKNVAEVLEILEGYGYETSAFQTT
                     AEKDSAKNEATRAALADFDLIIAAGGDGTINEVVNGIAPLEKRPQMAIIPTGTTNDYA
                     RALKVPRGNPVEAAKVIGKQQTILMDIGLAKNQKNGFHQEHYFINIAAAGTLTELTYS
                     VPSQLKTMFGYLAYVVKGAELLPQVQFTPVRVEHDEGVFEGSVSMIFVALTNSIGGFE
                     QIVPDAKLDDGNFTLLMVKTGNLFEILHLIRQVLDGGKHIESDLVEYIKTKSLSIENL
                     NPDNRLLLNLDGEFGGEAPVRLHNLSNHIEFFADTDLVSDHAITLDTDQMNREDMAKR
                     FIEETDHIDEAIK"
     gene            27814..28014
                     /locus_tag="ACHU66_000202"
     CDS             27814..28014
                     /locus_tag="ACHU66_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR3388518.1"
                     /translation="MSLGALALGLACLGVSIGEGLLVASYLSSTARQPEMQSKLMAGV
                     FLGVAFIEGTFFVTLAMTFVLK"
     gene            28051..28767
                     /gene="atpB"
                     /locus_tag="ACHU66_000203"
     CDS             28051..28767
                     /gene="atpB"
                     /locus_tag="ACHU66_000203"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR3388519.1"
                     /translation="MEEHLSPTLTLGPVTFDLTMVLVSVITISIIFLLVFWASRRMEL
                     KPKGKQNVLEYVYELTINFTKGNLGDAEAKRYSLFFFTVFTFLLVANNLGLMTKLETA
                     EGHNLWTSPTANMAYDFGLATIATVFCHVEGIRRRGFKAYLKSFVTPWAMAPMNILEE
                     VTNLVSLALRLYGNIYAGEVLVSLLLQLSQQSALAYPIAFALNVVWTAFSVFISCLQG
                     YVFIMLVSMYLNKKISSESE"
     gene            28785..29291
                     /gene="atpF"
                     /locus_tag="ACHU66_000204"
     CDS             28785..29291
                     /gene="atpF"
                     /locus_tag="ACHU66_000204"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR3388520.1"
                     /translation="MATTITMQSSTILGNFILVTASFAVLIILIRVFAWDKITGIFEE
                     RANKIANDIDAAEEKLTAAANLVQQREDELVQGRIESQKIIQDAVERAKLEKKRILEQ
                     ADVEIQGLKQKAQLEIEAEKREAQENLRVQVAELAVDLASKIILEDLDQQAHSNLIDR
                     YLDKLGDK"
     gene            29292..29825
                     /locus_tag="ACHU66_000205"
     CDS             29292..29825
                     /locus_tag="ACHU66_000205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR3388521.1"
                     /translation="MNARENAIVQKYALSFVEKVSDHADIWDMYDQISDLISIIHDSK
                     LNRILLSATVSREEKADFVRTVRQSSFWQINDLIEDVIRDGHADLLLETLERVQLQIS
                     KFKNEFEARVVSVYPLTEAQKERLRHLVEQRFSLRVRNITEELDQSLLGGFIVTVNHK
                     VIDASVRTQLKDVRKKL"
     gene            29829..31349
                     /gene="atpA"
                     /locus_tag="ACHU66_000206"
     CDS             29829..31349
                     /gene="atpA"
                     /locus_tag="ACHU66_000206"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR3388522.1"
                     /translation="MESGVLLINAQEISALLKQQIEGFQPDFDYTETGVVTYIGDGIA
                     RAQGLDNAMSGELLVFENGTIGMAQNLETNDVGIIILGQFTDIREGSVVRRTGKIMEV
                     PVGSALIGRVINPLGQPVDGLGEIRTSKTRPIEYPAPGVMQRKSVNEPLQTGLKAIDA
                     LVPIGRGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLR
                     QYGALDYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYEGKHVLIVYDDLSKQAVAYR
                     ELSLLLRRPPGREAYPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAY
                     IATNVISITDGQIFLKDDLFNSGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLA
                     SYRELEAFTQFGSDLDAATQAKLNRGRRTVEVLKQPLHKPLPVEKQVLILYALTNGFL
                     DSVPIDDILAFEEELYSYFDLHYDGLLDVIRTTKDLPDTDELNAAIQAFKDQSVFK"
     gene            31361..32239
                     /locus_tag="ACHU66_000207"
     CDS             31361..32239
                     /locus_tag="ACHU66_000207"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR3388523.1"
                     /translation="MAGSLNEIKSKIASTKKTSQITGAMQMVSASKLAKSEQLAQSFQ
                     IYASKVRQITTDLLRGELMASDTSNPMLIRRPVQKSGYIVITSDSGLKGSYNSSILKA
                     VMGMIEQDHDSKDEYEIIAIGSMGADFFRARGINPVFELRGLADNPSFEEVQKIISKS
                     VEMYKNELFDELYVCYNHHVNSLTSQVRVQQMLPVEDLDHNEADGYTATFELEPNREV
                     ILEQLLTQYAESTIYGAILDAKTAENAAGMTAMQTATDNAKNVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            32259..33665
                     /gene="atpD"
                     /locus_tag="ACHU66_000208"
     CDS             32259..33665
                     /gene="atpD"
                     /locus_tag="ACHU66_000208"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR3388524.1"
                     /translation="MSSGKITQVVGPVVDVAFAAEDKLPEINNALVVYKNDDSKQKVV
                     LEVALELGDGVVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EESFPADFEREPIHKKAPAFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     LGIYPAVDPLASSSRALAPQIVGEEHYAVAMEVKRVLQRYQELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAEQFTGMPGSYVPVAETVKGFKEILDGKHDHLPEDAF
                     RNVGSIEDVVAKAAKMKF"
     gene            33675..34091
                     /locus_tag="ACHU66_000209"
     CDS             33675..34091
                     /locus_tag="ACHU66_000209"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR3388525.1"
                     /translation="MGQMTVQIVTPDGIKYDHHAAFVLVKTVDGEMGVYSGHEELIAV
                     LEIGEMKVRRVDDENHVDWIAVNGGIIEVNKDIITVISDSAERERDIDISRAERAKLR
                     AERELEEAQTARNIDMEMRATVALQRAINRIRVGKH"
     gene            34151..34375
                     /locus_tag="ACHU66_000210"
     CDS             34151..34375
                     /locus_tag="ACHU66_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFR3388526.1"
                     /translation="MVISILQFFSHILFIYLAHHLLVTTVDWSRWLKVTGDNQRKINL
                     LILFLAIALGYLVSTFFLELLMVGRSFANM"
     gene            34400..35689
                     /gene="murA"
                     /locus_tag="ACHU66_000211"
     CDS             34400..35689
                     /gene="murA"
                     /locus_tag="ACHU66_000211"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR3388527.1"
                     /translation="MDTIIVTGSKNGLKGQVEIEGAKNAVLPLLAATVLASQGQTKLT
                     QVPILSDVYTMNNVVRGLGVRVKFDEETKTIDIDATGKLGSETPYKYVSQMRASIVIL
                     GPILARNGYAKVSMPGGCTIGSRPIDLHLKGLQAMGAKIKQRAGYIEASAENLQGAHI
                     YLDFPSVGVGATQNLMMAATLAQGTTVLENAAREPEIVDLAIFLNKMGAKVKGAGTET
                     ITITGVEELTGAEHQVVQDRIEAGTFMVAAAMTGGDVLVKDAVWEHNRPLISKMLEMG
                     VEVTEEAEGIRVRSQVDKLKPVTVKTLPHPGFPTDMQAQFTALMAVAKGESTMIETVF
                     ENRFQHLEEMRRMDLHSEILRDTAIITGGLALQGAQVMSTDLRASAALILAGLVAEGE
                     TIVGKLTHLDRGYYRFHEKLAALGATIERVSGEDENG"
     gene            35682..35873
                     /locus_tag="ACHU66_000212"
     CDS             35682..35873
                     /locus_tag="ACHU66_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR3388528.1"
                     /translation="MDKDNLSYVGKNLILVAVVLLIAILVFVLGLMVGYGVIGDGDNV
                     FAVLSPAKWQELIGKFTGK"
     gene            35903..36742
                     /locus_tag="ACHU66_000213"
     CDS             35903..36742
                     /locus_tag="ACHU66_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFR3388529.1"
                     /translation="MAKKNVRKQAMSLLSLLATIVVVAGGYLLSDNKSSNVADYSYYV
                     NQSKASEGTPSQELASSVLTEDVKKQLGNSIEWNGAGAFIINGNQTDLDASVASVPYG
                     DNKTKTVQGETVPTVANALLAKSTRQYKDREETGNGRTSWTPAGWHQIHDLTGEYDHA
                     VDRGHLLGYALIGGLSGFDASTSNPKNIAVQTAWSNQANDQTSTGQNYFESQIRRALD
                     KNKRIRYRVTLVYAESEDLVPVGSHLEAKAADGSLEFNVFVPNVQSGLSINYHSGEIA
                     VNQ"
     gene            36929..38482
                     /locus_tag="ACHU66_000214"
     CDS             36929..38482
                     /locus_tag="ACHU66_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR3388530.1"
                     /translation="MEKHVEQRVEDVTYNKAKLWQIILFAMNNSSTNIYLVAFTFVTY
                     LSTGVLGLAALFVSQLMGYIRIFDGFIDPTIGVIIDKTDTKFGKYRPILVLGNIITAA
                     SLFMMLGLSGVNESLRLPLFILVLIIHKIGYSLQQTITKAGQVALTNDPKQRPIFNIV
                     DGIATTVLMTGSQVVVSGTLVPKHGGFTPSFFQELFLGVVVISAVLGILAIIGIWEKD
                     NKKYFGLGEKTQETKLKDYWKVIKGNKPLQVLSISAAFVKFVAQLFGDSVIGVMLYGI
                     IFGDYALSGKMSMLLILPGILVTVGAAQLARRKGLRFAYITALQLGIAGLVILAAMLF
                     SAKPGMLDLTNWNLYTIGFFVVYIVARYASSAPSGLVLTMGADITDYETSVSGRYLSG
                     MIGTIFSLTDSIASSFAPMVVGFVLAGIGFGKEYPTVETAFSTDLRMALIVLLTVIPA
                     AVLSVSLFLMKFYKLDREEMARIQEKIHVMKAAKDEERVRAIAKNVPLSDMDYVDVTQ
                     YKVDQYDDK"
     gene            38538..39566
                     /locus_tag="ACHU66_000215"
     CDS             38538..39566
                     /locus_tag="ACHU66_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR3388531.1"
                     /translation="MKKILAFGEVLLRLSPPQYQTLTQATSLDCQFGGSELNVLASLA
                     QLGHSVELVTALPKNEVGKMAEHFLFARQIGQKYVIRNEGRLGLYYYQKGFSLRPSQV
                     TYDRDYSAFSLSKEEDYDLEGMFDGVDWFHVSGITVALNPDIYRLAFRLMEMAKGQGV
                     KVSFDLNYRESLWSSFEEAREKLSPFVGLADICFGLEPIQLFNESGKDLKDELGLKRP
                     YENKEVLLAVVKGLAETYSLQGLAFTQREMTYNNEYLLKAYLYQDGILYETEKESIQV
                     LDRVGTGDAFTAGIILGYLQEKSPREILDLGMASFQFKHTIEGDINVISQSDIDLLLK
                     KGSREIKR"
     gene            39582..41381
                     /gene="uidA"
                     /locus_tag="ACHU66_000216"
     CDS             39582..41381
                     /gene="uidA"
                     /locus_tag="ACHU66_000216"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR3388532.1"
                     /translation="MLYPIQTKTRSVYSLNGIWKFKQGNNDVHTLLDTDEVMVVPSSF
                     NDLVVDKDKRRFVGDNWYELMVALPVVSADEELVVRFGSVTHQAKVYADGQLIGEHKG
                     GFTPFECLIPSNLYDADQFRLTVCANNELNYTTLPVGNYSEEVDENGQIVKTVKENFD
                     FFNYAGIQRTVHLYKRPQNRIEDIVIRTELNQDLTQAVVNVDVKTVGQYDSIRMSILD
                     QEGQEVGLLENGQMVIEHPRLWEVLDAYLYTAKVELFDGENLLDTYSEQFGIRSVAVE
                     NGQFFINGKPFYFKGFGKHEDTFINGRGFNEAANLMDLNLLKDIGANSFRTSHYPYSE
                     EMMRLADRLGIVVLDEVPAVGLFQLFNAALNLTGDSEEVKSTWEVMQTKDAHELVIDE
                     LIARDKNHPSVVMWVVANEPAGHEKGARAYFEPLIQRMRDRDPSKRPVTLVNIMTATP
                     DKDEVMDLVDVVCLNRYYGWYVAHGDLKAAEKGLRQELETWQKLYPDKPILITEYGAD
                     TLQGLHSMWDIPYTEEFQVDYYDMNHRVFDSIPNLVGEQVWNFADFETTVGIIRIQGN
                     HKGLFSRNRQPKQIVREIKKRWTAIPNYHYKRK"
     gene            41427..42143
                     /locus_tag="ACHU66_000217"
     CDS             41427..42143
                     /locus_tag="ACHU66_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR3388533.1"
                     /translation="MAKPLVEKASERLLELILERGYEVGDKLPNEYELAQDLEVGRST
                     VREAVRSLATRNILEVRQGSGTYISSKKGVSEDPLGFSLVRDRVKLTTDLFEVRYLLE
                     PRIAERVAQFASDEDIARLEEIVLAIEEAVAAGDTKHLELDVKFHCMLAEMSGNLAMT
                     SLMPVINQSIHLINENYSNRQMKSDSLQAHRNILMAIKQRHPVAAYDSMLAHIISVRQ
                     VVLSEWFDKDMTLHGLNDHK"
     gene            42266..42886
                     /locus_tag="ACHU66_000218"
     CDS             42266..42886
                     /locus_tag="ACHU66_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR3388534.1"
                     /translation="MLKQLKENYFFAVIRGKDEEDAKEIARHAILGGIRNIEITFSTP
                     NAATVIKELQEEFSYDSSVVIGAGTVMNLKLAQAAIDAGASFLVSPHFDKEIQDLAQE
                     AEVFYFPGCATATEIVTASQAGCPIIKLFPGGVLGPGFIKDIHGPVPQVDLMPSGGVS
                     VENVADWKKAGACAVGVGSALASRVQAEGYESVTTIARSFVAALEG"
     gene            42888..44288
                     /gene="uxaC"
                     /locus_tag="ACHU66_000219"
     CDS             42888..44288
                     /gene="uxaC"
                     /locus_tag="ACHU66_000219"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR3388535.1"
                     /translation="MSFNDKNFMLKNEPAKELYGKIAELPIYDFHCHLDPKEIFEDKV
                     YEDIVDLWLGGDHYKWRLMRANGISEEEITGSASKLDKFKAWARTLERAFGNPLYHWS
                     HLELRQVFGVEELLTEENAERLYHQLNAYLQEHRVSPRKLIADARVTFIGTTDHPLDS
                     LEWHKRLAEDSTIDTVVAPTFRPDEAFIEHRNFNLFMKRLEEVTGVAVRDFASFVEAL
                     GQRVSYFAQHGCRASDISFTAISYEEATLEELDEILLARLAGKEAGQSSINKWQTAIF
                     RELCRLYKKHGFVTQVHFGALRNNHTGLFEKLGADVGVDSIGDQTCLTVNLNRLLDNL
                     VKENALPKMIWYNLNPSYNIALANTLANFQSNEEGLRSQLQFGAGWWFNDTKLGMIDQ
                     MNAYAEQGMLANFVGMLTDSRSFLSYQRHDYFRRILATYVGQWIVDEEVPEDYNRLGQ
                     FVEAISYYNAKEFFEQ"
     gene            44303..45403
                     /locus_tag="ACHU66_000220"
     CDS             44303..45403
                     /locus_tag="ACHU66_000220"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR3388536.1"
                     /translation="MKMSFRWYGKKDPVTLEEIKAIPGMQGIVTAVYDVPVGQAWPLE
                     NILELKKMVEEAGLEITVIESIPVHEDIKQGKPNRDELIENYKTSIRNVGAAGIPVVC
                     YNFMPVFDWTRSDLHHPLPDGSTSLAFLKSDLAGVDPVADDLNLPGWDSSYSKEEMKA
                     IIENYRQNISEEDLWANLEYFIKAILPTAEEAGVKMAIHPDDPPYGIFGLPRIITGQE
                     AVERFLNLYDSEHNGITMCVGSYASDPKNDVLAMTEYALKRNRINFMHTRNVTAGAWG
                     FQETAHLSQAGDIDMNAVVKLLVDYDWTGALRPDHGRRIWGDQTKTPGYGLYDRALGA
                     TYFNGLYEANMRAAGKTPDFGITVKTVGNKES"
     gene            45405..46244
                     /locus_tag="ACHU66_000221"
     CDS             45405..46244
                     /locus_tag="ACHU66_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR3388537.1"
                     /translation="MTRVIEFKDKVVVITGAGGVLCGYLAKEFAKAGAKVALLDLNEA
                     AAQVFVDEIAAAGGVAKAYKSNVLSKENLEGVRQQVLADFGPVDILINGAGGNNPKAT
                     TDNEFHEIGLPADTKTFFDLDEAGINFVFNLNYLGTLLPTQVFAQDMIGRSGANIINI
                     SSMNAFTPLTKIPAYSGAKAAISNFTQWLAVHFSKVGIRCNAIAPGFLVTNQNRGLLF
                     DESGQPTARANKILTNTPMGRFGEAEELVGGVFFLADENLASFVNGVVLPIDGGFSAY
                     SGV"
     gene            46244..47044
                     /locus_tag="ACHU66_000222"
     CDS             46244..47044
                     /locus_tag="ACHU66_000222"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFR3388538.1"
                     /translation="MMKTDYVFCVDSDGCAMDTMTYKHKLFFGPLAADVFDVKDREPF
                     LAEWNRVNLYSRERGINRFVGLVKGLDFAGVTGIDNLKNWVATTDSLSNASLERLIEE
                     TPSKDLELALEWSTQVNQAIKHYSGPVLAFIGVHKGLEKLSQLGKVYVVSSANKEAVE
                     EEWTDQGLLDFVTELYCQDRGKKEDVIKILIEEGYCPDKIMMIGDSPGDLKAAELNGV
                     HFYPILVGQEMQSWADLTETIADEFAHQAFTGEKETELIQAFWNNLDD"
     gene            47094..48884
                     /locus_tag="ACHU66_000223"
     CDS             47094..48884
                     /locus_tag="ACHU66_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFR3388539.1"
                     /translation="MTHLVDLRKKPYSLDEEAISWIEETIAQMTLDEKIGHLFVNMGS
                     QRTEEYLTGVLNDYKIAAVRYNPGPAADIWEQNYILQTKSKIPLLIAANTESGGNGAV
                     TDGTKIGDEIKIAATADPRYAYEMGKVAGLEAAAVGCNASFAPIMDLSRNWRNPIIAN
                     RTWGANVDQVIELSKEYMRGIMEHGIVPFAKHFPGDGIDERDHHLSFASNPMTKSEWM
                     ETFGRIYGEMADAGLPGIMAGHIHLPNVEKEMHPERELDDMLPASLNKTLLDELLRGE
                     LGYNGAIVTDASHMIGMTASMSRRELLPTAIEAGCDLFLFFNDPEEDLQWMKEGLENG
                     LLSEERLHDALRRTLGLKAKLGLHLFEGRRQEIMMPKEEALALIGRDEAKQLAKEVAD
                     KAITLVKAKQEGIFPVNPERYKRILLVEVDGYKGGFGAMINAGKKRAADTLKELLEAR
                     GHQVSIWENTEARIAKLPEEERPAAIANVYASKRPIAEITDNYDLIINLVDVNSGGTT
                     QRIIWPAAKGTPDQPFYVHEIPTIVVSVQHPFALADMPQVATYINAYDGLPVTLEALV
                     AKLAGESDFTGVSPVDAYCGLVDTQIWRGK"
     gene            48895..50619
                     /locus_tag="ACHU66_000224"
     CDS             48895..50619
                     /locus_tag="ACHU66_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFR3388540.1"
                     /translation="MPRLVDLRKKPYCLNDEQITWVEDTLAHLTDEEKIGQLFVNLFF
                     FGGDAFSGNNLSNQELMNRYHIGGARYMNGSPEQVQGLINELQSYSKIPLLVAANCDS
                     GGDGAVKGGTYISSGAQSEASRDPRIPYLAGLVSAREETALGVNVNFDPCVDIVQNWR
                     NTIVNTRAYGTNADDVIAYTSAYLKGLTAERDVIQCIKHFPGDGTEERDQHLVLGVNE
                     LSPEEWDKSFGRVYRHHIEAGVEMIMAGHIALPYYQQRLNPTLKDEDILPATLAPELI
                     QDLLKEKLEFNGLVITDASHMLGMTAAMRREDYVPAAIAAGCDMFLFFNNLEEDFEFM
                     LNGYRKGVITEERLHDALRRILGLKAKLNLHIKQAEGTLLKSADELAIIGCEEHLAWQ
                     REAADKAITLLKDTQKNLPISPETHRRIRLYYLEGEKEGIMAASTEVVDNLKAALEAR
                     GYEVTVNDGTTRIKGKTLQYREEVDLALTVANVIGYGAQNNYRIQWKTAMSNEVPWYV
                     HEVPTIFVSLNYTTHLHDVTMVKTAINAYHHNINTIESLLDKLEGKDDFYGVPNENVW
                     ANKWQAKL"
     gene            51074..52117
                     /gene="pheS"
                     /locus_tag="ACHU66_000225"
     CDS             51074..52117
                     /gene="pheS"
                     /locus_tag="ACHU66_000225"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR3388541.1"
                     /translation="MSNIEQQLAELSQTTLEKLKEIQHQGEKELQDLRVAVLGKKGSL
                     TDLLKGLKDLSNDMKPIVGKQVNEVRDVLTTAFEETAQKVAAAKIQQQLASETIDVTL
                     PGRQVKVGKRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDM
                     QDTFYITEEILMRTHTSPVQARTMDQHDFSKGALKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGENVSMGDLKGTLEMIIKKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     DGCNVCKKTGWIEILGAGMVHPQVLEMSGIDSTKYSGFAFGLGQERIAMLRYGINDIR
                     GFYQGDVRFSEQF"
     gene            52646..53167
                     /locus_tag="ACHU66_000226"
     CDS             52646..53167
                     /locus_tag="ACHU66_000226"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR3388542.1"
                     /translation="MQIDPICLEEVSVLQELAKQTFAETFAHDNDPKELEAFFEESYS
                     LEVLTSELSDPNCQHYFLRIDHQIAGYLKLNQGPAQTEQELENAFEIQRIYLLQAFQG
                     RGLGKVLFEFALEKAEQSSCDWVWLGVWEHNYKAQKFYSKYGFEKFGQHEFAVGDKID
                     VDWLLKRPLHQKG"
     gene            53169..55568
                     /gene="pheT"
                     /locus_tag="ACHU66_000227"
     CDS             53169..55568
                     /gene="pheT"
                     /locus_tag="ACHU66_000227"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR3388543.1"
                     /translation="MLVSYKWLKELVDFDATSHDLSEKMSTTGIEVEGVEQKSAGLSK
                     LVVGQVVSAEPIPDTHLNTCQVNVGDEITQIVCGAPNVKPGIKVIVALPGARIAGNYK
                     IKKGKIRGVESLGMLCSLSEIGVSDSVVPKVYADGIYHLPEDAVVGEPVFSYLDLDDE
                     IIELSITPNRADALSMRGVAHEVAAIYDSKVNFKPVVLEESDKKASDVIEVAIESDKV
                     AAYTARVIENVTIAPSPQWLQNLLMNAGIRPLNNVVDITNYILLYFGQPMHAFDFDKF
                     DGKKIVARQAKEGENLVTLDGEERDLITDDIVISVGDKAVALGGVMGGANTEIDNNSK
                     TVVLEAALFDGKSIRKTSGRLNLRSESSSRFEKGINVATLTEAMDTAAAMIAELAGGQ
                     VLSGIVSAGSVDTSDVPVTATIDYVNRSLGTNLDYAQIADIFRRLGMETTGDASQFTV
                     AVPRRRWDIRIPADLVEEIARIYGYNNLPTTLPKEDGTAGELTLTQNIRRQVRSLAEG
                     AGLTEIISYALTTPEKAVEFAARPTTVTELMWPMTVDRSALRQNMVSGMLDTVAYNVA
                     RKNKNLALYEIGKIFEQSGNPKEDLPQEINKFALVLTGLVAEKDFQTPAVPVDFFHAK
                     GILEAIFGHYKLAVDFVATSEIAALHPGRTAEIHLNGAVIGFVGQVHPQTAKDYGIPE
                     TYVAEINLDAIEEALQPAQPFTEISKFPAVSRDIALLLSSDVKHQDVLDAIAGAGVKR
                     LTKVTLFDVYAGSNIEAGKKSMAYNLTFQNPADSLTDEEVAKYMEKISKALVELGAEI
                     R"
     gene            55623..55889
                     /locus_tag="ACHU66_000228"
     CDS             55623..55889
                     /locus_tag="ACHU66_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFR3388544.1"
                     /translation="MNTQDIQEKFFREGKLIVIPKKLKSKQVLFAYLQEELAKKGSAF
                     TEKEVNAFLAEIYDDYAILRRYLVDYGYLSRDQYGLEYRIEEKR"
     gene            complement(55910..56212)
                     /locus_tag="ACHU66_000229"
     CDS             complement(55910..56212)
                     /locus_tag="ACHU66_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388545.1"
                     /translation="MYEFVLEYGSFPVKLIDGFVNNRSEIPDFLKEDEEMIARLNEIN
                     ELFHQLFLTIECKFDYIGKQFPDKIEQLRELYHPLADDLLAKYGNQIELKIEPFIL"
     gene            56405..57148
                     /locus_tag="ACHU66_000230"
     CDS             56405..57148
                     /locus_tag="ACHU66_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HFR3388546.1"
                     /translation="MKTRIVQKQFKRKETKYIVDKKTFELLEKELRQYMMADEFATST
                     ITNIYFDNEDFDMIQDSLAKKNGREKIRMRVYDAIPSESSQAFLEIKKKENKIGYKYR
                     LTSNPVSVANYIENGVIDSTIKDDKVNSELEMLRERYGTIKPKMYIYYDRVSYKGIED
                     KKVRLTIDKNLLYRDYDVDAMEGKFGKNLLDPTKVIMEVKVPEERPDWLVALLEKYQI
                     EKQSFSKYGNAYKLAHNITGEEVSQHAVV"
     gene            57135..57812
                     /locus_tag="ACHU66_000231"
     CDS             57135..57812
                     /locus_tag="ACHU66_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFR3388547.1"
                     /translation="MQLFNSIFSTANSNITLAQMSMVFGVSLSMGVLLAAVYKYKSNY
                     TKEFVITLSLMPALIAVIIALVNGNLGAGVAVAGTFSLIKFRSAAGSSKEMLAVLLAM
                     AIGLATGMGFLGLAVFMTVILSACILIFENISFAQVNQNRRHLLVTVPMNFDYDQFFE
                     NQFGKSCKQSDLLSLKYKKKKEALVLEYQVLLDKKITDKRLVDTVLSAGPLDVVLNKQ
                     MPKKKYL"
     gene            complement(57861..58343)
                     /locus_tag="ACHU66_000232"
     CDS             complement(57861..58343)
                     /locus_tag="ACHU66_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFR3388548.1"
                     /translation="MTKKPVQLATICYIDNGKEFLLLHRNKKENDVHQGKWIGVGGKL
                     EPGETPQACAIREIFEETGLTVTKHALKGVITFPDFTPNTDWYTYVFKITGFEGSLID
                     CNEGDLEWVPYDQVLSKPTWEGDRHFQEWLLEDKPFFSACFRYDGGKLLDYSVDFYEE
                     "
     gene            58510..59073
                     /gene="folE"
                     /locus_tag="ACHU66_000233"
     CDS             58510..59073
                     /gene="folE"
                     /locus_tag="ACHU66_000233"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR3388549.1"
                     /translation="MSKQEQIEQTIYQLLELLGEDPNREGLLDTPKRVAKMYLEMFNG
                     LEEDPKDQFTAVFSEGHEEVVLVKDIPFHSMCEHHLVPFYGIAHVAYIPSKGRVTGLS
                     KLARAVEVASRRPQLQERLTHQVAHALQDALEPEGVFVMVEAEHMCMSMRGIRKPGSK
                     TVTTVALGKYKEDAILRRELLSMIHNK"
     gene            59082..59894
                     /gene="folP"
                     /locus_tag="ACHU66_000234"
     CDS             59082..59894
                     /gene="folP"
                     /locus_tag="ACHU66_000234"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR3388550.1"
                     /translation="MSIEQLANQVTIMGILNVTPDSFSDGGHYNEVESALVQVEKLLA
                     EGATVIDVGGESTRPGATFVSEEDEIARVVPIIRAIKENYDCLVSVDTYKTGTARAAL
                     EAGADILNDVWAGLYDGDMLALAAEYKVPIILMHNQKDESYADVVGEVKNFLAERAQA
                     ALDAGVSADQIWLDPGFGFSKNVQHNLDLLQGLEQITALGYPVLFGISRKRVVDYLLG
                     GNSLPTDRDQATAALSAWAVQKGCKMVRVHNVAANRDIVKVWDQLTSGGQHG"
     gene            59887..60249
                     /gene="folB"
                     /locus_tag="ACHU66_000235"
     CDS             59887..60249
                     /gene="folB"
                     /locus_tag="ACHU66_000235"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFR3388551.1"
                     /translation="MDKISLNKCRFYGYHGAFKEEQVLGQIFTVDCDLFVDLTAASQT
                     DNLEDTVHYGMVFETIKAVVEGKPYILIEKVAGVICQELFDQFPKVEKIRLAIYKENP
                     PIAGHYDSVGIELERERP"
     gene            60246..60734
                     /gene="folK"
                     /locus_tag="ACHU66_000236"
     CDS             60246..60734
                     /gene="folK"
                     /locus_tag="ACHU66_000236"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR3388552.1"
                     /translation="MKHLAYLSIGGNMGNRQAYLQAALDKLASHPGCQLGLVSNIYET
                     PAWGKTDQADFLNLACQVYTDLSAQDFLSACQNIEQELDRVRIEKWGQRTIDLDIIFW
                     DEEKIQEENLIVPHPYAHERAFVLNPLAEIASDYRHPILKKVVSDLLAELGEQKDIKI
                     FK"
     gene            60785..62095
                     /locus_tag="ACHU66_000237"
     CDS             60785..62095
                     /locus_tag="ACHU66_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFR3388553.1"
                     /translation="MTILATLGVLLAIVAIIYWTSKNLHVIIAAPLASLIIILTNQMN
                     VLEVMLGKEQSYMAGLAGFLINNFAIFMLGSILARYMEASGATQTIANSILKVMGKDS
                     PYKGLLAITLIASILTYGGVSIFVVIFTLLPLSRPLFKELNINWALFPIPVFLGAGTY
                     TMTTLPGAPSIQNVIPTKVLGTSLTAAPVISLAASLTLLVFGLLYMAYCLKKSLANGE
                     TYTEEEDETAVVVADKTPNLFLSVLPLFSLIGTIFLMSKTPNVLAIGLLVSILLSALI
                     FYPYLPNQKEILNAATTASIVPAFATSSTVAFGTVLTLSAGFAVIQEWIQQIPGSPLI
                     SLSVSTALVSGIIGSSSGAVGIASSNFLPAYLEMGINPELLHRVVVVASAILTVVPQS
                     GVMITFHNLSKLSMKRGLKYSFILVTVGHLLALFVILLLAPLLY"
BASE COUNT        17930 a        11477 c        14881 g        17886 t
ORIGIN      
        1 aaatatggaa aaatcacata gaaaacgctt gattcataaa ggggaagaat attcatatta
       61 tgcattggat tcgatatcaa tggaagaaaa agtggatatt cattcacttc cctatactat
      121 tcgtatttta ttagagagtc ttttaagaaa agaagatggt gtggatgtta caaaaaatca
      181 cataatggag ttgcttcatt atcaggctgc atctcctaaa ggggaaatcc cctttaaacc
      241 tagtcgtgtt attttgcaag attttacagg tgttcctgtt gtggtagatt tagcctccat
      301 gcgtgatgcg gtggtgaaag caggtggaaa ccctgaattg attaacccgg aaattccagt
      361 ggatttagtc atcgaccact ctgttcaagt tgattttttt gggactgaag atgctctgga
      421 gaaaaatatt gccttagagt tcgaaagaaa taatgaacgc tatgaatttc tgaaatgggc
      481 agagaattcc tttgaaaatt atcgcgctgt gcctccagcg acaggaatta tccatcaggt
      541 aaacattgaa tttctaagcg atgttattat caacaaggat ggtttgcttt atccagattc
      601 tatgtttggt acagacagtc atacgaccat gattaacggt attggtgtac ttggttgggg
      661 tgtaggtggt attgaggcgg aagctgcaat gttgggtgaa gcatcttatt ttcccgttcc
      721 tgaggtgatt ggtgttcgcc tagctgggca attgcctaag gttgtcactg caacggattt
      781 ggcgcttaag gtcactcaac ttctacgtca ggaaaatgta gttgggaagt ttgtcgaatt
      841 ttttggacca ggtttatctt ctcttgcatt agcggatcga gcaactgttt caaatatggc
      901 tccagaatac ggtgcgactt gtggctattt cccgatcgat ggagaaactt tacattatat
      961 gagattgacc aaccgttcgg aggagcacgt ggagttgaca gaagcctata ccaaggctaa
     1021 ttatttattc tatgatgctg aacgttttcc gtcttatagc aaggttttag aattggattt
     1081 atcgacagta gtcccatcta tttctggtcc taaaagacca caggatttga tagaattaac
     1141 agatgcaaaa gcagagtttc aggctagttt aatacgtgaa gtaggtgtgc gtggatttgg
     1201 tttggaggaa gcagagttag ataaaacagc cacggttaag tatgttgaag gagatgagca
     1261 aattcaaaca ggtcatgttg ctattgcggc gattacctcg tgtacaaata cctccaatcc
     1321 gtatgtcctt ttggcggcag gattactggc aaaaaatgcg gtagaaaaag gtttggcagt
     1381 ttctaagacg gtcaagacgt cattggcacc aggctcaaaa gtagtgactg gctatttgaa
     1441 aaaatcaggt ttgcagacct atctagatgc cttagggttc aatttggttg gttatggttg
     1501 tacaacttgt atcggtaact caggtgatct gcgtcctgaa gtagcagagg cgattaagga
     1561 agaagattta ttggtttctg cggtattatc tggaaatcgt aactttgaag gacggattaa
     1621 tccattggtt aaagctaatt tcttagcgag cccaccgctt gttgttgcct atgctattgt
     1681 gggaaatatg aatgtagatt taacaaggga tccgctgggg tatgatgaga agcaacaagc
     1741 tgtctacctt gcggacatta tgccaagtcg tgaggaggta gatgactaca ttgagcgtta
     1801 tgtgactcgt gacctttata aggaagaata ccaacaggta tttacagata gtcaggcttg
     1861 gaacgcgatt gaaacaaaaa cagataagaa ttacaattgg aattcatctt ctacctatat
     1921 tcaaaatccg ccctattttg acaacatgca ggtagattta tccataaaac cactggaaaa
     1981 tttatctgtt ttggctaaat ttggcgattc tgtaacgaca gatcatattt ccccggcagg
     2041 gaatattgca cgattgagtc ctgcagctcg ttatctcgaa gagaatggca ttgtttataa
     2101 ggattttaat tcttatggaa gtcgtcgtgg aaatcatgaa gtcatgatgc gtgggacttt
     2161 tgcaaatatt cgtataaaga atgagctggc agctggaaaa attggtggtt ggacaagagt
     2221 tggcgatgag attcttccta tctatgatgc agccatgaaa tataaggccg ctggtatcgg
     2281 tagtattgtt attgctggta aagactatgg gatggggtca agccgtgatt gggcagccaa
     2341 aggttcaagt ttacttggtg tgaaggctgt tcttgctgag tcatttgagc ggattcaccg
     2401 ttcaaattta gtgatgatgg gtgttcttcc tttacaattt ttagaaggtc agtcggcaga
     2461 aagtcttggc ttgactggac atgaaagcta tacaatcgac ttgccagaag atgtcggtgt
     2521 tggtcaaatt gtaacagtcc atgcgcaaat agacgatgtt acaaaggagt ttcaagctct
     2581 ggtgcggttt gatgcagagg cggatattcg ttattatcgc cacggaggta ttttacctat
     2641 ggtagtaaga aagaaactag gaggaaaggt atgacagaaa cgaatgggtt aaaagatgcg
     2701 attgcctgtg atacgcgaat cagtgcaatc gaaaatgacc gcttgtccta tgcagggtat
     2761 gacattgctg aattgatgga gaataacgct tcttttgagg aagtcatcta tcttctgtgg
     2821 aacctgcatt tgccaactca gattgaatta aattattttg agaaaagtct gcgaaaagaa
     2881 tatgcgattt cagatgcggt agagcaatgt attctgattc aatcacggcg acatttgcat
     2941 cctatgagcg tgttgcgttc gacagtatct ctccttggag tatataatgt acatgctgaa
     3001 gagaattcgt tagaagcttt gtatgaacaa tctattcaga ttatgggcaa ggttccgacg
     3061 attattgcaa catttgctcg tttgagacgc ggattggttc caattgaacc gcggtctgat
     3121 ctgggttttg cggcgaattt tctttatatg ctgaatggtg aggagccgag tgatttacaa
     3181 atcaaagcgt ttaataaagc tttggtgctt catgctgatc atgaattgaa tgcatctact
     3241 tttgctgcgc gtgtcacggc atcaacgtta acggatttat attcttgtgt taccacggca
     3301 attgggactc tgaaaggctc attgcatggt ggtgcaaatg aacgagtgtt tgacatgttg
     3361 ctggctattc gcgagagtgg tgatgccgag agatatttgc agaagaagct ggattcgaag
     3421 gaaaaaatta tgggatttgg ccatcgagtc tataaaacgg ttgaccctcg ccaagagtat
     3481 ttgaaggaga tggcgcgtga tttgacattc ggaacagacg atgaagcgtt ttatagattg
     3541 tcagaggaag tagaaagctt tattaaaaac aaaaaaggtt taattccaaa cgtagatttt
     3601 tattcggcga cagtttacca tgtgctaggt attgatagtg atatttttac attgattttt
     3661 gcaatgagcc gtatggcggg gtggattgca catgtccagg aacaacggaa aaataataaa
     3721 ttgattcgtc cgcgctcttc ctacttaggt ggtaggaatt tggtttacat cccgattgga
     3781 aagaggtaaa tatggcagaa aaaatcctaa tgagagaggg acggttaatt gtcccagatt
     3841 gtcccgttat tccctatatt gaaggagatg gtattggcag ggatatttgg tcagtcagtc
     3901 aaaaagttat tgatgctgca gtgaaaaaag cctatgcagg tcaacgttct attcagtgga
     3961 gggaggtttt ggcaggagaa aaggcttatg aattaacggg aacttctttg ccgaatgaga
     4021 cactggaaat aatccgtgag catttagttg caatcaaagg tcccttggga acgccgattg
     4081 ggaaaggtca tcgttctctt aatgttgcac ttcgtcaaga attagatttg tatgcctgtg
     4141 tgcgcccgat tcgctatttt aagggagtgc caagtccgct gaaagagcct gaaaagacgg
     4201 atattactat ttttcgtgaa aatacagaag atatttatgc aggtattgaa tgggaggctg
     4261 gttcagatga ggttgccaaa gtcatttcct ttctgcaaga agaaatggga gtggtaaata
     4321 tccgttttcc agaatcttca agtattggta tcaaaccgat ttcggaagaa gggagtaaac
     4381 gcctcattcg ctctgcaata gaatatgccc tagaacatca attgaagata gtgaccttag
     4441 ttcataaggg aaatattcaa aagtttacag aaggcggttt ccgcaagtgg ggttatgaat
     4501 tggcgaaaga agaatttgct acagaacttg cagaggggac tttggtagta aatgacatta
     4561 ttgcggataa ctttctgcag cagattttat tggcgccgga aaacttccaa gtggttgctc
     4621 ttacaaatct aaatggagat tatgcatcag atgctcttgc ggctcaggtc ggaggaatag
     4681 gcatctcacc gggtgctaat attaattatc agacaggtca tgctatcttt gaagcgacac
     4741 atggaactgc tcctgatatt gcaggaaagg atttggctaa tccatgttcc ctcttgcttt
     4801 cggcttgtat gatgttagaa tatataggct ggaaagaagc ggctacttta attattcagg
     4861 ccttggagga cgcttttata aataaaaatg ttacgcttga ttttgctaaa attttggacg
     4921 ttgctcatca atctactagt caattttcag aagttctaat agattctatt taatgaagaa
     4981 aaagtaaaat gaacctatct ttttatgacg gaaacatgaa agaaaggtaa aaaaatcttg
     5041 aaaatgattt caaaaagtgg tataatgtcc tttgtaaggg ttatcacaaa taactcacaa
     5101 ttttgtaaaa ataaaggaga atccaaatat ggcttcaaaa gacttccaca ttgtggcaga
     5161 aacaggtatc cacgcacgtc cagcaacttt gcttgtgcaa acagctagta aatttgcttc
     5221 agacatcact ttgaactaca aagaaaagtc tgttaacctt aaatctatca tgggtgttat
     5281 gagtcttggt gttggtcaag gcgctgacgt aacaatctct gctgaaggtg ctgatgctga
     5341 cgacgcaatc gcagctatca ctgaaacaat ggaaaaagaa ggattggcat aagaatatga
     5401 cagaaatgct taaaggaatc gcagcatcag atggtgttgc tgttgctaag gcatatctac
     5461 tcgttcagcc agatttgtcc ttcgaaactg ttacagttga agatacaaat gcagaggaag
     5521 ctcgtttgga tgctgctcta gaagcatctc aaaacgagct ttctgttatt cgtgagaacg
     5581 cagtagctag cctaggtgaa gaggccgcag ccgtatttga tgcacacttg atggttcttg
     5641 ctgacccaga aatgattggt caaatcaagg aaacaattcg tgcgaagaaa acaaacgctg
     5701 agactggttt gaaagaagtt acggatatgt ttatcgctat ctttgaaggt atggaagata
     5761 acccatacat gcaagagcgt gccgcagata ttcgtgacgt agccaaacgt gtattggcac
     5821 atttgcttgg tgttcgcttg ccaaacccag caactatcga cgaagagtca atcgttatcg
     5881 cacatgactt gacaccttca gatacagcgc aattaaacaa acagtttgta aaagctttcg
     5941 taacgaacat tggtggtcgt acaagtcact cagctatcat ggctcgtaca cttgaaattg
     6001 ctgctgtttt gggtacaaat aacattactg aaatcgttaa agacggcgat gtattggctg
     6061 ttaacggtat cacaggtgat gtagtgatta acccttctga agaagtgatt gcagaattta
     6121 aagtagctgg tgaagcctat gctaaacaaa aagctgaatg ggctcttttg aaagatgctc
     6181 aaacagttac agctgacggc aaacactttg aattggcagc aaatatcggt acaccaaaag
     6241 acgttgaagg tgtaaatgat aacggtgcag aagctgttgg tctttaccgt acagaattct
     6301 tgtacatgga ttcacaagac ttcccaactg aagacgagca atacgaagct tacaaggctg
     6361 ttcttgaagg catgaatggt aagccagtcg tagttcgtac aatggatatt ggtggtgata
     6421 aggaacttcc ttacttcgat cttccacacg aaatgaaccc attcctaggt ttccgcgccc
     6481 ttcgtatctc tatctctgag actggtaacc aaatgttccg cactcagttg cgtgcccttc
     6541 ttcgttcatc agtacatggt aaacttcgta tcatgttccc aatggttgcc ttgttgactg
     6601 aattccgtgc agcaaaagca atccttgaag aagaaaaagc aaacttgttg gctgaaggtg
     6661 tagcagttgc ggatgatatc caagttggta tcatgattga aatccctgca gcagcaatgc
     6721 ttgcagacca atttgccaaa gaagttgact tcttctcaat cggtacgaac gaccttatcc
     6781 agtatacaat ggctgcagac cgtatgaatg aacaagtttc atacctctac caaccatata
     6841 acccatcaat ccttcgcttg atcaacaatg ttatcaaggc tgcacacgca gaaggtaaat
     6901 gggctggtat gtgtggtgag atggctggtg atcaacaagc tgttccactt cttgtaggta
     6961 tgggcttgga tgagttctct atgagtgcaa catctgtact tcgtacacgt agccttatga
     7021 agaaacttga tacagctaag atggaagaat acgctcaccg tgccttgaca gaatgtgcaa
     7081 cggcagaaga agttcttgaa cttcaaaaag agtatgttga tttcgattaa tcgttgaaaa
     7141 aagcagggaa ttgaatcctt gcttttttac tgttcacaaa aagacttgtc ggagcaagtc
     7201 tttttgtgtg cttagttttt ctgataaaac ttattgtgtt ggtagatagg gtcaaaggta
     7261 agattgatgc ctaatttacg aaggacactc ttgtcttcat cggataggat aacagtcgag
     7321 tgggcttcgc taccatttaa ttggcctaat tctttcatgg cacgtgcagc aagagggcta
     7381 tcttgtgcag tgatggcaag ggcgttaaga atttcgcttg catcgagtcg tgtatttgtc
     7441 ttatccagat aatctgcctt caattcttga attggacgga tgtagtttgg ttcgatgaga
     7501 agtgtttcat catcgatgtt agcaatttgc ttgatggcgt tgagaaggac agttgcagtt
     7561 ggcttgagga ggtcggatgt cttacctgtt acaatctctc cagttggcaa ctgaatagca
     7621 agggcagggc tagttgttag ctctgctttt tcgcgcgcag cgataacaac cttacgatct
     7681 gctggcgtca caccgacttc attcatgagg agttcaatct tcttaactgc ttgctcgctc
     7741 acccgctctg ccttgaaatc aaccaaggtc tggtagtagc ggcggatgat ttcttgcttg
     7801 gatgcttcaa tcgcagcctc ttcgtccacg atggaatagc ccaccatgtt aacgcccatg
     7861 tctgtcggtg aggcgtatgg tgatttgttc aaaatacgtt caaaggtacg gttgaggaca
     7921 gggaagacct cgatatcacg gttgtagttg accgcagttt tgccgtaagt ttgcaggtgg
     7981 aaggggtcga tcatgttgag gtcgtccagg tcagcagttg ctgcttcata ggccaagtta
     8041 acgggatggt ggagtggtaa gttccacact gggaaggttt caaacttagc atagcctgat
     8101 gttactccat gcaattggtc gtggtaaagt tgggagatac aggtcgccaa tttacctgaa
     8161 ccaggtccag gtgcggtgac aacgacgaga ttgcgactag tttcaatgta gtcatttttg
     8221 cccatacctt ctggtgaaat gatgtggtcg atatcagaag gatagccctt gattgggtag
     8281 tggagatagg acttgatgcc gtgtttttcc aactgcttgc ggaaggcgtc tgcggctggt
     8341 tgattgcggt actgggtaat gaccacagaa ccaacataaa tatcaatatc attgaaggtg
     8401 tcaatcaagc ggaagacttc ttggtcataa gaaataccca agtcaccacg tgccttagaa
     8461 tgttcgatgt tgcttgcatt gatggcgatg acgatttcta cttggtcttt taactcttgg
     8521 aggagtttga ttttgttatc tggttcatag ccaggtaaaa cacgggctgc gtggaaatct
     8581 tccagcattt ttccgccaaa ttccatatag agcttgcctt caaactgagc aattcgttct
     8641 aaaatatggt cacgttgcaa attcaagtat ttagttgaat caaatgcgat tttcttcatc
     8701 aaataagtcc tccaaatgtg tattaatctc tgggaaaaac aaaaatagct ccctaaaact
     8761 agggagcctt ttctccgacc tctcaaaaga ggtgcccgaa caatactata acaaaccgtg
     8821 ctaatcttgt caagaaaaag tcagacagaa gaaaagttaa acaagtgttt aaaaaatatt
     8881 taatttttat aaagttaaat attgacttta ttaaaataaa gtgtaaaata atattaaaca
     8941 aatatttaat cgatgtttaa ggagatgttt atgaaaaagt tgattcacaa taaattattt
     9001 ctatctagct ttgtagcaga cttgatttct aactttggag atactcttta ctatctggct
     9061 ttgatgaacc atgttttact cttagcagat accaagtttg ccctggctat gattaccgca
     9121 tctgaaacct tgccgatttt ggctggtctc tttatcggga tttgggcaga caagaccaaa
     9181 aacaaattgg ataccattct tgcaactctg gtggttcgtg tcggtctcta tgcctttgtt
     9241 ggcttcttga tgggatttgc accagccctc tgggttgtgg cggtggtctg cgtcatcaac
     9301 ttcctgtctg accttgctgg tcaatacgaa aacggtctct atatgccagt cagcttgcgg
     9361 gttgtggctg ctgaagaccg tgaaacagct atggctttta agatgacggt gaaaagtctt
     9421 ttgcaaattg ccttccaagc tagcggtgct atcctcatcg gattcatgtc ttaccagaat
     9481 ctcgccttct tcaatgcagg gaccttcttg gtcagtcttg ccatcatggc aaccctccgt
     9541 ccagctcttg ccaagctact gaaggaaaat cctatccaag aagctgagca aacagaaaca
     9601 gaagcaggac ttatcaaggg tatgcgtaac agtctcaaag aatcctacca agctgttcag
     9661 aatattcttg ttctcaaagc gtcaattgtg accattgcca gcctgaatgc catttttaca
     9721 gcactttcac cattagttat tctcaatatg aaggagttct cagactttgt gattgtcaat
     9781 gcaggaacaa ccgttgcttt gatttccatt ttcttctcag ttggtagtat cttggggtca
     9841 agtatcggta tggctttgtt caaaaatgtt agcttgatca atctcctcaa attctctaca
     9901 ttgatgccag tcttgatttt ctcgggtttc tttctccaca atatttatgt ggtattgacg
     9961 gttatttttg tgacagctat gactttgggt gtcttcaatc cgaaaatgaa tgccttggtc
    10021 ttaaatgaac tgcctgaaga taaattggcg acagtcggtg gagggattga tgctttctgc
    10081 cagattggta tggtagcagg tcaagccttg gtagccctta tggtaacaat cttatctgtc
    10141 acaagtattt ccctcatctt cctcttgctt tctgtgggac tagtaagcta tactattctt
    10201 gtgggttgta aacctaaaaa tgaagtagct caagtttaga aagtatgcgt atgaaattag
    10261 attatcgaga ttaccgcagt gaaattgtgg aaaaactgtt cattcccttg attgtcaagg
    10321 agcgtgagga gccgtttgaa gcggatttgt cacagaagga gaaagacatt ctcaaagatg
    10381 ccttggacat tcgcaatgaa gtagaaaaca agctggcgga ttttcgtcag gaagtcaatg
    10441 aggtctttgt ttggggccat atctttacaa tcctgcacac cttgtatttc tacctcttgg
    10501 atcaaggaca ggatccaaag acagtcgagg aggcttgtca gctgattttg accttatcgc
    10561 aagaagaggt agaagatgct atgcggacca tgctagcgtc tgaaaatgac ggtcatcgtg
    10621 aaaaggacct cagcctcatg gaactcttgg aaaagacaga taaaaagcca gcagaaaagt
    10681 ggtattggtc actagccctt cgcaatccct tggaaactgt tgagcggtct gtcgatctcc
    10741 tgaacaagct actcccaatc tatcagccgt attttgaagg ggcaagagca gagcgggaag
    10801 cctttgctag agactttgac attgaacaac tctacagaga atccaagcag ttagccatga
    10861 ccagcttgga tagcttgggt gtagaaactg cccaattctt cgttctcagt ccatggaatt
    10921 attggtttgc ttattatggc aatgaacaat ttgactatat gaaagttgct cttttagctt
    10981 cctgccgaat tgatcagatt atgctgtcaa atgacgagtt ggatctggat gatttgacaa
    11041 cggctcttaa agtcatcagc gatagtacgc gttatcaggt cttggtggag ctgaccaagc
    11101 ctcatgccaa gagcaaggac attgcagaac gcatggctat tacaggggcg gccgtatcct
    11161 tccacaccca aaagctaatt aacggtgact tacttctctt caatacaaaa aatagcgatg
    11221 tcaaatacag tgtcaatcgt gaacttctcc aagaggtcat tgccaaattg acagaggatt
    11281 ttgatttgta aaaaaagcct tgtaagttag atcttacaag gttcttttgt gcttatactc
    11341 ttcgaaaatc gaaatcagac gttgttgact tgatttgatg aacttcagtt ctatccgcat
    11401 ctgcgtcgcc tagtctgttt ttgattttca ttgagtatta atgattaacc agctgagtag
    11461 ttggcaagtg atgatggtga taaggaagac taaccaccct tgctgtattt tggtcaggga
    11521 actagtttct aggaagaaaa agctgatttc gactgggata acatggataa aataataata
    11581 gctggcgtat tcttttagaa attggaattt tttcttgcgg aagaggtctg tggttaaggt
    11641 ccaagcgact agataggctg tgaacggaat caggctaaat agaaagttct tgtcgtagcc
    11701 ctgattgaga tagatgacag tggcttcaaa agctaaaagg gctgtgcaga caagtagttt
    11761 ggttttttgt cggtgttgga aaatggggtg attgagtttg ttggctagat aaaagcctat
    11821 caggacaaaa atcggtgcgt agaagaggcc gttgcgactg gtgaaaaaga aggttttgta
    11881 gttgtcaaat gcggtcagga aaatcgattc tgctaggtag gcggagtagg tttcgactga
    11941 gcctagaaga tagagaaggc aggctagaca agctgtgatg cgccagcccc atttctccaa
    12001 ggaaagatgg acgaggataa gacctagaaa gaaagcgggc atgtaccaga gttggtagct
    12061 ggtgccggta taggtcagtc cgactaggag tgcgagggga tagtagagga aggggatttc
    12121 ttggagtgtt aggtagatga ggtagaaggg caggtagagg atggaccagg tcaggtattg
    12181 tttgctgtag cggatgaggt attgtttgga gaggctgggg tagttgcgtc ggaggaagaa
    12241 gctggcggtg accatgaagt agggaacgac catgcggcta aagattgatt tttggatgaa
    12301 gtggagctgg tctgcttcaa atagtcttcg acagtgaagg gcaacgacga ggatggctcc
    12361 gatgaactgg atgagggaga gcagtgatgt attgagttgt tttgtcttat tttgcatgct
    12421 aacgttcttc ttttttcttc tattttatcg aaatctgatg aatatgtaaa tgtttgcaaa
    12481 aagatgtgga gaggtgctgg ctgaaactta gttgacaaga gaagtccagt ggaaaatatg
    12541 gtataatgtt aggacggagt aaatgtaaac catctatttc gtttaagtta gaaaggagca
    12601 tatagatgaa agatttacat gcaaaattat tggctgattt tgggattgat ttttcggatt
    12661 tgaatttatt agaaacggct tttacccata cttcttatgc caatgagcat cgccttctaa
    12721 aaatttcaca taatgagcgc ttggaatttt taggagacgc tgttctccaa ttaatgattt
    12781 ccaaatatct ctatcaaaaa tatcctgata gacccgaagg tgagatgtcc aagttgcgtt
    12841 cgacctttgt acgtgaggag tctttggctg gtttttcacg tgcttgtggt tttgatcggt
    12901 ttcttcgtct tggtaagggg gaagagaagt ctggagggcg aaatcgcgat actattttag
    12961 gagatgcttt cgaggcgttc ttaggagctc tgctattgga caaaggggaa aagaccgtgg
    13021 aggaatttat ccataaggtc atgattcctc gtttagaaaa agggaatttt gagcgcgtga
    13081 ccgactataa gactgctttg caggaaattt tgcaggtcaa tggtgagatt gctatttcgt
    13141 atcaagtaat agctgagtct ggacctgccc atgataagac ctttgaagtg gaggtatctg
    13201 ctgatcggcg tgtgattggt cgtggtcgtg gtcgctctaa gaaattagct gaacaagccg
    13261 ccgccaaaaa tgctgtggag gctagaggat aggtatgtat ctaaaatcaa ttgaaatgca
    13321 gggctttaag tcctttgcgg ataagactaa ggtagtcttt gatcgtggag tgactgctgt
    13381 tgtagggccg aatggttctg gcaagtccaa tattacagaa agtctacgtt gggccttggg
    13441 agagtcgtct gctaaaagcc tacgtggagg caagatgcca gacgtcatct tttcgggaac
    13501 agaaagccgc aaggctctta actatgcctc ggttgtcgtg accttggata atagcacagg
    13561 ttttatcgcc aataaacaga aagaaatcaa ggtagaacgc cacatttatc gatctggtga
    13621 tagtgagtac ttgattgatg ggcaaaaggt tcgtctacgg gatattcatg atcttttcat
    13681 ggatacgggc ttgggacgtg attctttctc tatcatttca caagggcggg ttgaagcaat
    13741 tttcaattcc aagcctgagg aacgccgtgc tatttttgaa gaggcggcag gggtcttaaa
    13801 atacaagacg cgtaaaaaag aaacagaaag taagttggca caggcccagg gcaacttgga
    13861 ccgcttggat gatattatct atgaattgga taatcaggtc aaaccattag agaaacaggc
    13921 acaaactgct aagaaatttc tagaattaga tggtcaacgt aaggaattat acttagatgt
    13981 tttggtggct cagttgttgc ttggtaagga aaagttatcg gaaaaagaag ccgaactaga
    14041 gtctgtcaaa actgaattga ctagctacta caaacagcgt tctgagctgg agcaagaaaa
    14101 tcagaacttg aaagagaagc gccaccgcct ttcagaacaa ttggagcgtg aacaggcagt
    14161 tctgttggat ttgaccaagt taatcagtga tttggagcga aaaattgagg tccataaact
    14221 ggaatctagt caaaacgaat caagtcgtca agaagcgcag gcgcgcttgg aaaacttgtt
    14281 gactagacga gagcagttgg cagaacagat tgagcaaaag caagaaacgc ttgcgcaatt
    14341 agatagttcc ttgtctagct tgaaagacga tattgctgcg gtagacaaag aaatttccta
    14401 cttttcagaa gatcctgatc aggttttaga tcatctgcgt gaacagtatg ttgctctgat
    14461 gcaggaagag gcagaagctt caaacagtct gaccaagatt cagcaggata ttgccaatca
    14521 aatcagtctt tctgagagta aatctgaaga cttggcacgc ttgcagacgg aaaaacaaac
    14581 agctcaagaa gtacttgata agagtcggaa aagtttagaa gaagcagatc acgttttacg
    14641 ccagttgctt gagagctatc agattaaaaa atcggaatta gatcaaaccc agactgctta
    14701 tcaggctgag caaggtcgct tgtttgattt gctggatcag ttgaaaggca agcaggctcg
    14761 ccagtctagt ttagaagcta ttttaaaaaa tcactccaat ttctacgcgg gggttaaggc
    14821 tgtcttacag gcggcaccaa gcttaggagg aattattgga gcagtcagtg aacaattgac
    14881 ctttgatact cgttaccaaa cggctttgga aattgctctt ggcggtgcta gtcaaaacgt
    14941 catcgttgaa gatgaagcga ctgctaaacg agctatcgca tttttgaaag aaaaacggca
    15001 aggacgggca accttcttac ctctaacaac tatcaagcca cgtcagcttt ctggacagca
    15061 agtctcttta ctagaatctt cagaaggatt cttggggctt gcaagtgatt tggtgactta
    15121 tcagccaaat ttagatgcga ttttccaaaa tttgctaggc actattgcga tttttgatag
    15181 cattgaccat gccaaccagg cggcgcgtgc gaccaagttt caagtgcgta tggtgaccat
    15241 ggatggagcg gaaatccgtc ctggtggagc ctttgctggt ggtagcaatc gaaataacag
    15301 cacgaccttt atcaaaccag aactggatgc tcttttagga gaaatagcag agctgtctag
    15361 ccagttgcag gagcaagaaa gcttggttgc agctaagaaa actagcttag accaaaccag
    15421 agaagccttg gaaacgatta aggcggaggg ggaagaagct agattgaacc agcagagcgc
    15481 aaggattcat caggaacagg cagaaaatag actggctcag ctatcagccc agtatgacct
    15541 gcaaatgagt caggttagtc cgaccatttt gactgagcta gaagaggctg ctgctaagga
    15601 agaagtaaat gtccaagcct tgaatgagaa aaaactagcc ttggatcaac aaatcaatca
    15661 agtccgtgat aatcgcgaca gtattcaaga aagcttgcag aaactacaga ctcagaaggg
    15721 tcaactaact ttggaacagg ctgaactgac tagccaactt cgttttgaac agacagatct
    15781 gagacgtttg caagaagaaa aagtggttgc agataaggaa atttctcttt tagaggatat
    15841 gattgaccag aagctagaag ctcttgaaga tactagcatt gagattcttg aagaacaatt
    15901 gcaggccgca tctgataagc aaaaccagac caatcagatt ctaattcgct taaagtttga
    15961 gttggaagat attgatgggc agtttgaaga cttggaagaa agattgcaac aagctcggac
    16021 taagaatgat gatttgatcc gtaagcaggc taagttagag gcggattgtg agcaagcggg
    16081 cgataaatta cggactttac ttggtaattt gaccgagcat ttcaaattga gttttgaagc
    16141 tgctcagtct caggccaagg aagttgagaa tcttgcagtt gctgaacaaa gcctcaaaga
    16201 cctagaaaga gctattcgtg ctttgggtcc agtcaatttg gatgctattg agcagtatga
    16261 cgaggtcaat aaccgtctga ccttcctcaa tgagcaacgg acagatattt tgtcggcgcg
    16321 tgatttacta ctagatacta tccacgagat ggatgatgaa gtcaaagagc gcttcaaggt
    16381 tacttttgaa gctattcgtg aaagctttaa gcagacattc aaacaaatgt ttggcggtgg
    16441 ttcagcggat ttgattttga cagaaggtaa cctattgact gcgggtgtgg agatttctgt
    16501 tcaacctccg ggtaagaaaa ttcaatctct caatcttatg tcaggtggcg aaaaagccct
    16561 ttcggccctc gctctgctat tttccattat ccgtgttaag accattcctt ttgtaattct
    16621 cgatgaggtg gaggcagcct tggatgaggc gaatgtcaaa cgttttggcg actacctcaa
    16681 ccgttttgat aaggagagtc agtttatcgt cgtgacccac cgtaaaggga ccatggcggc
    16741 agcggatgcc atgtatgggg tgaccatgca ggaatctggc gtgtccaaga ttgtttccgt
    16801 taagctgaaa gatgtggaga aattatgatt aacaaaattt acgccagtga tatggatggt
    16861 acctttttac gagaagacca tagttttgat aaggaacggt ttcgtcgtat actgaatcag
    16921 tttaaagaaa aaggttattt atttgtggca acaagtgggc gatctatgca aagcctgaaa
    16981 cttgtttttg aagattttgt agatgaaatc ggttttgtgg cagaaaatgg ttcaatcgtt
    17041 gaatatcagg ggcagactat ctttatggat gatccaatta gtcctgaaat ttatctgcca
    17101 attatcgcgg gtattgatgc aggtcccttt ggtagcagtc gttccatggt gctgtctgga
    17161 cttgaaaact tttatttatt aaaaaatgct gagcctcagt ttttagaagc gatgacgaat
    17221 tattacgcac attttcgatt ggtagacaca tttgaagaag ttcgagaaga gattataaaa
    17281 atcaatgcca aattttctcc tgaggaaatg gacgatgcta gacgatggtt aaatgataca
    17341 tttgaaggtg taacggccat gacaactggt tttgacaata ttgatataat tccaaatggt
    17401 tcaaataaat cagttggatt gagtcatctc tgtgctcatt ttggaattac ccgtcaggat
    17461 gtagtggcat ttggtgataa tcaaaatgat ttggatatgt ttgactttgc aggtctggct
    17521 cttgcgacag aaaatgcaag agaggaagtg aaggcccagg cagattggat gattggtcat
    17581 tgcaatgatg gagcagtatt agcctattta gaggaggaag taaatggcaa ttaagttgat
    17641 tgcattggat ttagatggaa cacttctgac atcggacaaa cggatttcag aagctaataa
    17701 aaaggccttg caggcagcgc gtgagcgtgg agtttatgta gttctgacaa cagggcgtcc
    17761 tttacaagct atcggtactt ttctagaaga actagatctg ctaggggaga atcaatattc
    17821 gattacattt aacggtggtc tagtccagga aaatacaggt cgggttttgg ataaggttgg
    17881 cttcaccatt gaagatgtgc gcaccatccg tcgtgtgaca aatgatttgg acctgccttt
    17941 ggatgtcctg tatggtggcg atgtctattc tctaccatca gcccatgaat cactttatct
    18001 aacttgtaat ccattgctta ataaaattgt ggcaagcgat gaggaaatgc cagaagattt
    18061 tgtctacaat aaggcagttt cagctgtggc agcagacttt ttggatggac aaattccaaa
    18121 aattcctgcg gaacttcacg agcgtttcga gattttcaaa tcgcgtgata ttctgctcga
    18181 atggagtcca aaaggtgttc ataaagcaaa tggtttggct aagttgattg gacatctggg
    18241 gattgatcag tcagaagtta tggcctgtgg cgatgaagga aatgatcttt cgatgattga
    18301 atgggctggt ctgggtgttg ccatggctaa tgcgactgat gaaatcaagt ctgcggccaa
    18361 ccttgtcctg ccaaaaaaca atgatgaaga tggcattgct tgggtcatcg agcactatgt
    18421 attgaatgag gactagtatg ggattatttg atcgattatt tggacaaaaa aaacaagagg
    18481 agcaagtcgc tcttgttgaa gaagtggaaa gggagcagtc ggcttctagc gaagatacag
    18541 taactactga agttgagtca gctactcaag aaatggaaaa gttccagtct gaagtagcag
    18601 agactgaatt gacagccgaa caagaagctc agaaacaacg gaccttagac atgatggctc
    18661 aatattatgc agccaaagaa gccgctgcgg ctcgagtgaa ggaagcgcag gaacaaggct
    18721 ttgaccctgc tattcaaacc aaggtcaagg aagagcctgt tgaggaagtt gctccagtag
    18781 ggcaagaaag tgaacaggac aagtaccagc gaaccttgaa aaaaactcgt acaggatttg
    18841 gggcaaggct caatgaattc tttgccaatt tccgttcggt tgatgaagaa tttttcgaag
    18901 agttggaaga aatgctgatt ttatcagacg taggtgtgca agttgcttct acgctgacag
    18961 aggagctccg ctatgaagcc aagctacaaa atgccaagaa gacagatgaa ctccgtcggg
    19021 ttatcatcga aaaattggtg gatatttacg aaaaagatgg tcaattcagc gagcgaatca
    19081 atttccaaga tgatttgaca gtcatgctct ttgtcggtgt caacggtgtt gggaagacga
    19141 cgtctatcgg taagttagcc tacaagtaca agcaagctgg caagaaggtc atgttggtcg
    19201 cagcggacac cttccgtgcg ggtgcggtag cccagctggt cgagtggggt cgccgtgtcg
    19261 atgtgccagt agtcacagga cctgaaaagt cagacccagc tagtgtggtt tttgatgggg
    19321 taaaacgtgc cgtggcagaa ggcgtggaca ttctcatgat tgacacagca ggtcgcttgc
    19381 aaaataaaga aaaccttatg gcagagctgg aaaaaatcgg tcgcatcatc aaacgaaccc
    19441 tacctgatgc accgcatgaa accctcttag cactagatgc ctccacaggt caaaacgccc
    19501 tcagtcaagc caaggaattt gcaaaaatta ctccattgac tgggctagtc ttgactaagc
    19561 tagacggtac agcaaaaggt ggtgttgtcc ttgccatccg tcaggaactg gatattccag
    19621 tcaagctgat tggtttcggt gaaaaaattg atgacatagg tgaattcaaa tccgaagaat
    19681 tcatgcgtgg gcttttggaa ggattggtat agaaaaaagt cgttagttca atgaactagc
    19741 gactttttct atctgtatgc aacagtgagt aaatggcaat atccacaata ccccgattat
    19801 ttcgagcggc ttggcggaga gttccttcat aggtcatacc gactttttcc atgacacgac
    19861 cagaaggacg attttcacta tcgtgaacag ctgtaatccg taaacagccc acttcctcca
    19921 ataaatagtg attaacagct tcaagagctt cggccatata actttgcccc caccatttac
    19981 tgcccaacac ccaacctaat tcggcagctt gaactctttc atcaaggctg acaacggaaa
    20041 tatctccaat gggttgctga ttttctttga gctcaattac ccacctgtat gttttttcct
    20101 gtgaatagga ttgaagacag aactggacat agtcttcact atcttggagt gtctgatgag
    20161 ttggccaggt cagataggtg gtaacctttt catctgaagt ccagttttgg aaaactgatt
    20221 caacatcgct tgcttgaaaa gggcgtaaaa ttagacgttc tgtttctaaa atctgagttc
    20281 ctttatgttg catcttattt cctcctcaac aatgcctcaa tccagtctgg tgcataggca
    20341 gaagtacagc cttttggcac cttcatatcg gaatagacag cgagttcctg accgattaca
    20401 agtccctgtt ccaggtagtc gggataggca acggcaatct ccaccaagca ttggttcatg
    20461 gtccattgga taagcgggtc tgcggtttgt aaatgctggc gaatatggtc tagagtttct
    20521 tcaatttctt ggttttttgc tgtttttcca gcaaagtatt tggcacgaag gaaccaacct
    20581 aggcgttgaa aaatagcttg gtctttggca agcaaatctt cttcccaaac cctgctgtct
    20641 tttcgtttgc tgagaatgat ggaagccagc ttatcgagca cattgaccga aatttgttca
    20701 ttgaggcaat tctctaggtc agcttgggga agctggtcag gcttattttt tgcaatttga
    20761 atagctaaat attgtgcttc aaggacacct gtttgccaga gtggggcaaa taagtccagt
    20821 cgatttttat aggccttggc gataccagaa atagctccca ttggaacgcc gtagtagggc
    20881 ttagtttcgc caattttttc ataacgcttg agggtgccag catggctggc tgcttggagt
    20941 tgtgtgagaa tggtgtttaa ttcagtcata aatatctccc aaaaaaactg gattgctcca
    21001 gtttttatat gtagtcgttt agtttgcttt tagtacaagg caacgagctg taggctgtac
    21061 taaagtacgg caaagcgagt taacgaagta atcaaagata aactaaatga ctatttcaat
    21121 aagccacgtt cacgatacca tacatctggt gtccaaaccc acttgcaacc gtagctttct
    21181 agcaaatcaa aggttgcctc aggtcccata gttccagctg gataggtgtg gagtgggact
    21241 tggttgcttg cccacaaatc aacaatgcgg tcaatcaagc tccagcttgc tttcacttct
    21301 tcccaatggc taaagttggt agagtcgccg ttcaagacat cgaaaaatag tttttcgtag
    21361 gcttcgggtg aatttccaag agcagttgca ttgtggcgga agttgagttt ggcaggagta
    21421 agagcaaagt gggaaccaac ttccttgccg ttgatagaga gcatgaagcc ttctgttggc
    21481 tggatgaaga tggtcaatac attggcctca gaaggctgac caaaaatatc ttccgcatgc
    21541 ttgaaggtga tggtcacgcg cgtgcccttt tctgtcagac gtttacctgt acggaagaag
    21601 aaaggtacat cacggaaacg gtcagtatca acgaagaaga cacctcctgc aaaggtttct
    21661 gtctgagaac cttcggcaat atttggttca tccaagtagc taacgtagtc ttttccgtcg
    21721 atggaacctg ctgcatattg accacggatg aagttgcgtt tgaggtcctc atcagaaggc
    21781 tgacgaaggt gttggaagac cttaatcttt tcagcgcgga catcttcctc tttgaagctg
    21841 gctggcttat ccattgctag aagggatagg acttgaaggg catggttttg caccatgtct
    21901 tttaaggcgc cagaatgatc gtagtagccc ccacgatcct caacaccaat cgcttcagca
    21961 aatgtaattt ggacgttatc gatgtaatcg cggttccaaa tatgctcaaa aataatattg
    22021 gcaaagcgaa ccgcaaagat attttgtacc atttccttgc ctaggtaatg gtcgatacgg
    22081 tagatttggt cttcattgaa ggcagcggct aattcatcat tgagtttgct tgcagtttca
    22141 aggcttgttc cgaaaggttt ttcgataatc aaacgctcaa aaccttgtcc atcaacgatt
    22201 tgctcagatt tgaggtgttt ggcaatggtt ccgaaaaatt caggcgccat agagaggaag
    22261 aagaccttgt tgtgctgggt attgtatttt tcacacaagt cgtcctgcaa tttacgaaga
    22321 gctacataat gttccgtgtc attgacatcg tggctttggt agtagaaatg acttgcaaat
    22381 tcatgagctt gacgtggtgt gtcaggcaga tcacctagtg attcaatgac ggtttgctca
    22441 aaaaattcct tggtccaggg tctgcgagct gtaccaatta ctgcaaagtt ttccttgatg
    22501 tgtccagcct tatagaggcg aaataaagat gggtaaagtt tgcgtttggc aaggtcgcca
    22561 ctagcaccaa aaattgtaaa caatacatgt gatgacataa tttcattcct atctgttgaa
    22621 tatcattaat agtataccac aaattgtcag aaaattcact ttttgagttg gaattattag
    22681 aaaacacggt agacatacgg ggaatctggc tgttcaatct gaaccatcga ttgtaattca
    22741 agtgttggga gattttgttt caacgatgtg tggtaggaaa ccttgattgt tcctctggct
    22801 tttacccaag tattgttagg aaattctgtg gtagcccccg tagagagtaa gccatatacc
    22861 ccagaatcag caatgcagtg aataatacca aagcggaaaa ggaaaaattg ctggctattg
    22921 tcagggtcat tgtagacaaa tccaatcatt tcaatcgttt ttcctgaaaa ttccgtagga
    22981 tagtcgtaga ttgcttccat cacttccata tagttttcat tagtaacgat aatggtttct
    23041 tcagccaagt atctatcagc aatttcacgc atctggtttt gataggcaga ttttgtaaaa
    23101 taaatagaag tatcaggctt gagatactga acagtcgtcc cttcttggtt ttgtgtatta
    23161 gtatcgtttt ctgctgctag tgggaaatga tagcccttgg cagcaacagt tgttgaatcc
    23221 aggcttaccg taggaaagag ccaggctata gcaagaggaa tgagcagtag tccaacgctt
    23281 gatagtttgg caattttgct ttttaaatgg ctatgtactc gaatttcttt tacccagaca
    23341 tacaattgta cgatggcaag tatgaaagat aaaaccatgg atagataggc tagatagcta
    23401 tagtgcagat taatgtattg gtctaatttt ccagagatat aaagatacat ggtcatttca
    23461 aagtaacctg caagaattaa aaaacgaatc atactacacc tccaagtatc agactatatc
    23521 ccaaaataac gagagtgata gtaatgataa aaccgactat aaatttccct ttgaaataat
    23581 gtttcatcat gagcaagttt ttaacatcca ccatgggtcc gataactaag aaagccatta
    23641 ctggggcgaa tccgaagctg gatagtagtg atgcaccaat aaaggcatcg gcttcggaac
    23701 atagggaaag aagaaaggcc agcaacatca tcagtaaaat agctgttaat ggactgtgac
    23761 cgatggtagt caggacagcg gttggaatat agacttgtac gagactggca aatagacaac
    23821 caaagactag gtaacgtccc gtatcaaaaa attcatcaat ggcatgaata agtgcgaagc
    23881 caactttttg ccaactggtt ttatctgaat aaccatgctg gtgatggtca aacttgcttc
    23941 ctttaagaat tggtcctttt acgaaaaaac caagaatgat tcccaatact agggatacaa
    24001 tgatggctcc tagggctcta tagaaggcga aaagtaggga atttccaaaa gcggtaaagg
    24061 ttgcaaaaag gacgatagga ttaatcacag gcgccgttac taaaaaggga acagcagtat
    24121 aactcggcac ttttttctct aatagccgat tgacaatagg gacaatacca cattcgcaag
    24181 acggaaaaat gaagccgata aaggttccaa atagaatggc aagaaacttg tttttaggta
    24241 agagtttacg aaccttatct ggtgttataa acacatcgat aaaacctgaa ataatagctc
    24301 caatcaagac aaagggcaag gcttcaatca tgatagacag gaaaatagcc cccgcctgta
    24361 ggacggaggt aggtaattga tcaagaccaa acatattatt ttttatcctg attcaagttg
    24421 tttttctcgg aattttcttt tgattttgga tctggatact caatttcttc caatttttca
    24481 aatgaagcaa attcttccag catcttttct aaatcatctt gtcgtttata agttacagcc
    24541 atagggcacc tccaaaaatt ttatataaac attatacatt ttttactaaa aattatctaa
    24601 aggaatctgt ttttattgaa gcgcaaatat agttagcacc ctcaaatcat gatataatat
    24661 tcttatgaaa acaagaatat cagaattagt tagcgtgctt aatcaatacg ctaaagagta
    24721 ttatcaattg gaccaaccta gcgtatcaga tgcagaatat gatacgcttt atcgtgaatt
    24781 agtagagcta gaaacggctc atccagaact gattttacca gatagtccta cccatcgggt
    24841 cgggggaaag atgctagacg gatttgaaaa atatagtcat gtttatcctc tttttagttt
    24901 gcaggatgcc ttttctcgtg aagagttaga agcttttgac cagcgagttc gcaaggaatt
    24961 tcctcaagca acgtatatct gtgaattgaa gattgatggt ctgtctattt ccctgaccta
    25021 tgaagcgggt aatttagtcg tgggtgctac acgtggtgac ggtagtgtcg gtgaaaatat
    25081 cacagaaaat ctcaaacgag tggctgatgt tcctttgacc ttacccgaag ctgtggacat
    25141 cactgtccga ggtgagtgtt atatgccaaa agcttccttt gaccgtgtta acaaacagcg
    25201 tcaggaggct ggtgaagctg aatttgctaa tccgcgtaat gcagcagcag ggaccctccg
    25261 ccagcttgat acgggagtgg tggcccagcg tggtctcgct actttcctct accaagaggc
    25321 tagtccatct gaagcgacta gtcagtcaca agtactggag aaactggatg ctctgggatt
    25381 tgtgaccaac cacgaatatt gcttggcgga gtcgattgat gacacgtggg attttattga
    25441 aaaaatagca gagcgtagag atgatttacc ctatgagata gatggtgttg tcatcaaggt
    25501 aaatgattta gctatacaag aagaactagg atttacagtt aaagcaccac gatgggcagt
    25561 agcttacaag tttccagctg aggaaaaaga agcagaaatc ttgtcggttg attggactgt
    25621 tggccgaaca ggtgttgtga caccaacggc caatcttagt cctgttcaac tggcaggaac
    25681 gactgtcagt cgagcaactt tgcataacgt agattatatc gccgaaaaag atattcgtat
    25741 tggcgatacg gtcattgttt ataaggcggg agatattatt ccagcagttc tgaaagtggt
    25801 ggacaagtat cgaaaagagc aagaaatcat gcccattcct agtcattgtc cgtcttgtca
    25861 gagcgaccta caacactatg aggacgaagt tgccctccgc tgtatcaacc ctatttgtcc
    25921 tagccagctg atgagtaagt tggaacattt tgcaagtcgg gatgctatga atatcgcagg
    25981 gctagggtct tcaatcgttg aaaaactctt tggagcaggc ttggtacacg atgttgctga
    26041 tatttataaa ttaactgttg atgacctatt gaccttggaa ggtttcaaag aaaaatcagc
    26101 tgacaagctc tatcaagcca ttcaaacttc taagagtaac tcagcagaat gcctcctatt
    26161 tggactaggt attcgccatg taggtagtaa ggcaagtaag attttagtag aaaaatttgg
    26221 tgatttagaa actctagcat ttgctgatca agaagcaatt gcttcgctag aaggtcttgg
    26281 tcaggttatt gctaagtctt tgacaacttt ttttgctagt gagggtgcgc aacaactttt
    26341 agccgaactt aaggaagcta aggtaaactt aacctatctc ggacaagtag tagatgaaaa
    26401 tgctgcgcta tcgggtatga cagttgtact gactggtaaa ttggagcgaa tgaaacgaaa
    26461 cgaagccaaa gccaaattag aagctttagg ggcaaatgtg gcgggatctg tttctaaaaa
    26521 aactaatctt gtggttgccg gaacagatgc aggcagtaaa ttgaccaaag cacaggagct
    26581 aggtatcgaa attaaagatg aggcttggtt agagagcttg taaaggtaaa acgatggaag
    26641 aaagaaaacg tgctagacta atatataatc cgacctcagg tcaagaaatc atgaagaaaa
    26701 atgtggccga agtattggaa attttggaag gctacggtta tgaaacttca gctttccaaa
    26761 caactgctga gaaagactct gcaaagaatg aagctacacg tgcggcgctt gcagactttg
    26821 acttgattat cgcggctggt ggagatggaa caatcaatga agtcgtcaat ggaattgctc
    26881 cgcttgaaaa acgtccgcag atggccatca taccgactgg caccaccaat gactatgcgc
    26941 gtgccttaaa agtgcctagg ggaaatcctg tagaagcagc taaggttatc ggtaagcagc
    27001 agaccatttt aatggatatt ggtttagcca aaaatcaaaa aaatggtttt catcaagaac
    27061 attattttat caatattgct gcggcaggta cacttacgga gttgacctac agtgttccca
    27121 gtcaacttaa gaccatgttt ggctatttgg cctatgtagt aaaaggagca gaactcctgc
    27181 cacaagtaca atttacacct gttcgtgtag agcatgacga gggagtattt gaaggctcgg
    27241 tttcaatgat ttttgtcgcc ttaaccaatt caattggagg ttttgaacag attgttcccg
    27301 atgctaagtt agatgatggt aattttacct tattgatggt taaaacaggg aatctatttg
    27361 aaattctgca tctgattcgt caagtcttag acggtggcaa acacattgag agtgatttag
    27421 ttgaatacat aaaaaccaag agcctatcca tcgaaaatct gaacccagat aaccgtctac
    27481 tgctcaatct agatggagag tttggtgggg aagcccctgt acgtctgcac aacctatcaa
    27541 accatataga gtttttcgca gatacagatc tggtttctga ccatgcaatc actcttgata
    27601 ctgatcagat gaatcgagaa gatatggcca aacgattcat tgaagaaaca gaccatattg
    27661 atgaagctat taaataggaa gaaaagatga aatactcttt tctttttatt ttacaaaaac
    27721 gaattaaacg cttacaatgg cggtattttc ttgacaaata gatgttttag gagtatcctt
    27781 acatggtatt aaattttaaa aggagaattc gttatgagtt taggcgcatt ggcccttgga
    27841 ttggcctgtc ttggtgtaag tatcggtgag ggactgttgg tggcttcata ccttagttcg
    27901 actgcacgtc aaccagaaat gcaaagtaaa ttgatggctg gtgtattttt gggtgttgcc
    27961 tttatcgagg gaactttctt cgtaaccttg gctatgacat ttgtcttgaa atagtagaaa
    28021 atagagaaat agaaaagggg ggaacctcca ttggaagaac atttaagtcc aacccttacc
    28081 cttggtcccg taacctttga cctgaccatg gtattggttt ctgtcattac catttccatt
    28141 attttcttac ttgttttttg ggctagtcgt cggatggagc tcaagccaaa aggaaaacag
    28201 aatgttttgg agtacgttta cgaattgacc atcaatttta caaaagggaa tcttggcgat
    28261 gcggaagcaa agcggtattc cttgttcttc tttacagtct ttacttttct tttggttgcc
    28321 aataatctag gtttgatgac caaattggaa acagcagaag gacataattt gtggacttct
    28381 ccaacggcca atatggctta cgatttcggt ttggcaacaa ttgcaacagt attttgtcat
    28441 gttgagggta ttcgtcgtcg cggtttcaag gcatacctca aatcttttgt gacaccctgg
    28501 gctatggctc caatgaacat tttggaagaa gtgaccaacc ttgtgtcgct tgctcttcgt
    28561 ctgtacggta acatttatgc tggtgaagtg cttgtctctc ttcttctaca attatctcaa
    28621 caaagtgcac ttgcatatcc aattgccttt gctctgaacg ttgtttggac agcattttct
    28681 gtattcattt catgcttgca gggatatgta tttatcatgt tggtatctat gtacttaaat
    28741 aaaaaaatca gtagtgagag tgaataggaa agggaaagac aactatggca acaactatta
    28801 caatgcaatc tagtaccatt cttggaaact ttatcttggt aacagcctca tttgcagtac
    28861 ttattattct tattcgtgtt tttgcatggg ataaaattac aggtattttt gaagaacgtg
    28921 ccaataagat tgcgaacgat attgatgctg cagaagaaaa attaacagca gcagcaaacc
    28981 ttgttcaaca acgtgaagat gagttggtac agggacgtat tgaaagtcaa aagattattc
    29041 aagacgcggt tgaacgcgca aaactagaga aaaaacgcat tcttgagcaa gcagatgttg
    29101 aaattcaagg attgaagcaa aaagctcaat tggaaattga agctgaaaaa cgtgaagcac
    29161 aagaaaactt gcgtgttcaa gttgctgaat tggcagttga tcttgcaagc aaaattattt
    29221 tggaagattt ggaccaacaa gctcatagta acttgattga ccgttatttg gacaaactag
    29281 gagacaagta gatgaacgct agagaaaatg ccatcgtgca aaaatatgct ctatcatttg
    29341 ttgaaaaagt cagtgatcat gcagatattt gggatatgta tgaccagatt tctgacttga
    29401 tttccatcat tcatgatagt aagctgaatc gaatcttgct gtcagcgaca gtatcgagag
    29461 aagaaaaggc tgactttgta aggacggtac gtcaatctag tttctggcaa atcaatgact
    29521 tgattgaaga tgttattcgt gatggtcatg cagacttgct tcttgaaacc cttgagcgtg
    29581 tccaattaca aatcagtaaa tttaaaaatg aatttgaagc ccgtgtggtt tcagtctatc
    29641 cgttgacaga agctcaaaaa gagcgtctcc gtcacttggt agaacaacga ttttccttgc
    29701 gtgtgcgcaa tatcacggaa gaacttgatc aaagtctcct cggtggtttt attgtaacgg
    29761 ttaaccataa ggtaatcgat gcaagcgttc ggacacagtt gaaggacgtt agaaaaaaac
    29821 tttagaaaat agaaagtggt gttcttttga ttaatgcaca agaaattagc gctttactaa
    29881 aacaacaaat tgaaggcttt cagcctgact ttgactacac agagactggt gtcgtaacct
    29941 atatcggtga cggtatcgcc cgtgcgcaag gtcttgacaa tgccatgagt ggagagcttt
    30001 tggtatttga aaatggcacc attggtatgg cacaaaactt agaaaccaac gatgttggta
    30061 ttatcattct cggacaattt actgatattc gtgaagggtc agttgttcgt cgtactggta
    30121 aaattatgga agtaccagtt ggttcggctt tgattggtcg tgtcatcaac ccacttggtc
    30181 aaccggtgga tggtcttggt gaaattcgta ctagtaagac tcgtccgatt gaatatccag
    30241 caccaggtgt tatgcaacgt aagtcggtta atgaaccgct tcaaactggt cttaaggcta
    30301 ttgacgcttt ggttccgatt ggtcgtggtc agcgtgaatt gattatcggt gaccgtcaga
    30361 cagggaagac atctgtagct attgatgcta ttctcaacca aaaaggccaa gacatgattt
    30421 gtatctatgt ggctatcggt cagaaagagt caactgttcg tactcaggtt gaaacccttc
    30481 gtcaatatgg tgctttggat tacacaatcg tagtaacggc atctgcttca caaccgtctc
    30541 cattgctttt cttggctcct tatgctggtg ttgcgatggc agaagagttc atgtatgaag
    30601 gcaaacacgt cttgattgtt tacgatgatt tgtcaaaaca agcggtagct taccgtgaat
    30661 tatccctctt gcttcgtcgt cctccgggtc gtgaagccta tccaggggat gtattctact
    30721 tgcacagtcg tttgctagag cgttcagcaa aggtttcaga tgaattgggc ggtggctcca
    30781 ttacagctct accattcatc gaaacacaag cgggagatat ttcagcttac atcgcgacca
    30841 acgtgatttc tatcacggat ggacaaatct tcttgaagga cgatttattc aactctggta
    30901 tccgtccagc cattgatgcg ggttcttctg tatcacgtgt aggtggctct gcacagatta
    30961 aggccatgaa aaaggtggca ggtacccttc gtatcgactt agcttcttat cgtgaattag
    31021 aagcctttac gcaatttggt tcggatttgg atgcagcaac tcaggccaaa ctcaaccgtg
    31081 gtcgtcggac tgtggaagtg ttgaaacaac cattgcataa gccacttcca gttgaaaaac
    31141 aggttttgat tttgtatgct ttgacaaatg gcttcttgga ttcagtgcca attgacgata
    31201 ttttggcatt tgaagaagaa ctgtactcat attttgattt gcactacgat ggtcttttgg
    31261 atgttattcg tacaacaaaa gatcttcctg atacagatga attgaatgct gctatccaag
    31321 ctttcaaaga ccagtctgtc tttaagtaaa ggagtgaata atggcaggtt ctctcaatga
    31381 aatcaagtca aaaattgcat ctactaagaa aaccagtcaa atcaccggtg ccatgcagat
    31441 ggtttcggct tctaaactag ccaaatcaga gcaattagcg caatcctttc aaatctacgc
    31501 tagcaaggtt cgtcagatta caacggatct cttgcgtgga gaattgatgg cttcagatac
    31561 aagcaatcca atgctgattc gtcgtccagt tcaaaaatct ggttatattg tcattacctc
    31621 tgacagtgga ctaaaaggtt cttataattc cagtattttg aaggctgtga tggggatgat
    31681 tgaacaggac catgactcaa aagatgagta tgagattatt gccattggta gtatgggagc
    31741 tgatttcttc cgtgctcgtg gtatcaatcc tgtctttgaa ttgcgtggtt tagcagacaa
    31801 tccaagtttt gaagaggttc aaaaaatcat ttctaagtct gttgaaatgt acaagaatga
    31861 actctttgat gagttgtatg tttgttataa ccaccatgtc aatagtttga ccagtcaggt
    31921 gcgtgtacaa cagatgcttc ctgtagaaga tttggaccac aatgaagcag atggttatac
    31981 agctaccttt gagttggaac caaaccgtga ggttattttg gaacaactct tgactcaata
    32041 tgcagaatca actatttatg gagcgatttt ggatgccaaa actgctgaaa atgcagcagg
    32101 tatgacagcc atgcagacag cgacggataa tgctaagaat gtcattaacg atttgacaat
    32161 ccaatacaat agggctcgtc aggcagcgat tactcaggaa attactgaga ttgtagctgg
    32221 tgcctcagcc cttgagtaat agaaatagag gaaatagaat gagttcaggc aaaattactc
    32281 aggttgtcgg accagttgta gacgttgcgt ttgcagcaga agataaactt cctgagatta
    32341 acaacgcact cgttgtatat aaaaatgatg attccaaaca aaaagtcgtg cttgaagtgg
    32401 ctttggaact tggtgatggc gttgtacgga ccattgccat ggaatcaacg gatggattga
    32461 cacgtgggat ggaagttctc gatacaggtc gtcccatctc tgttccagtc ggtaaagaaa
    32521 cgctggggcg tgtcttcaat gtgttgggag ataccattga ccttgaagag tcttttccgg
    32581 cagattttga acgtgagcct atccataaga aagcgccggc ttttgacgaa ttatctactt
    32641 caagcgaaat tttggaaaca gggattaagg ttatcgacct cctagcacct tatctaaaag
    32701 gtggtaaggt tggtctcttc ggtggtgccg gtgttggtaa aaccgtcctt atccaagaat
    32761 tgattcacaa tattgcacaa gaacacggtg gtatctctgt atttaccgga gttggcgagc
    32821 gtacccgtga agggaacgat ctttactggg aaatgaaaga atcaggtgtt attgaaaaaa
    32881 cggccatggt atttggtcag atgaatgagc caccaggagc ccgtatgcgt gttgctctta
    32941 ctggtttgac cattgcggaa tacttccgtg atgtggaagg gcaggatgtt cttctgttca
    33001 tcgataatat cttccgtttc acacaggctg gttcagaagt gtctgccctc ttgggtcgta
    33061 tgccatcagc cgttggttat cagccaacac ttgcaactga gatgggacaa ttgcaggagc
    33121 gtattacctc aaccaagaag ggttctgtta catctattca ggctatttac gtacctgcgg
    33181 atgactatac agacccagct ccagcgacag ccttcgctca cttggattca acaactaact
    33241 tggagcgtaa gttgactcag cttggtatct accctgcggt ggatccattg gcttcgtcat
    33301 cacgtgctct tgctccacaa atcgttggtg aagagcacta tgcagtagct atggaagtaa
    33361 aacgtgtcct tcaacgttac caagaattgc aagatattat tgccattctc ggtatggatg
    33421 aattatcaga tgaagagaag accttggttg gtcgcgctcg tcgtatccaa ttcttcctct
    33481 ctcaaaactt caacgttgcg gagcaattta caggtatgcc aggttcttat gtgccagtag
    33541 ctgaaacggt gaaaggcttt aaggaaatct tggacggcaa acacgaccat ctaccagaag
    33601 atgccttccg aaatgttggt tcaattgagg atgtggtcgc taaagctgct aaaatgaaat
    33661 tctagaggtg gcttatggga caaatgactg tacaaatcgt aacaccagac ggtatcaaat
    33721 atgaccatca tgcagcattt gttttggtca aaacggttga tggtgagatg ggtgtctatt
    33781 ctggacatga agaattgatt gcagttttgg aaatcggtga aatgaaagtg cgtcgtgttg
    33841 atgatgaaaa ccatgtagac tggattgcgg tcaatggtgg aatcattgaa gtcaacaagg
    33901 atattattac ggtcatttct gattccgctg agcgggaacg ggatattgat attagtcgtg
    33961 cagaacgtgc caaattgcgc gcggaacgtg aattggaaga agcacagact gctcgtaata
    34021 ttgatatgga gatgcgtgca acagtcgccc tccaacgtgc gataaatcgt attcgtgttg
    34081 ggaaacatta atctatatgg gattgaactt ggttcagttc cattttttaa tttcatggta
    34141 taatggaaac atggtaattt caattttaca gttttttagt catatattgt ttatctatct
    34201 ggctcatcat ctcttggtta caacagttga ttggtcacgg tggttgaagg tgacggggga
    34261 taatcagcgt aaaatcaatt tgctgattct ctttctggca atagcactgg ggtacttagt
    34321 ctcgactttc tttttagaat tgttgatggt gggacgctct tttgcaaata tgtgatttaa
    34381 gataagaaaa ggataagaaa tggatacaat tattgttaca ggtagtaaaa acggtttgaa
    34441 gggacaggtt gagattgagg gggcgaagaa tgccgttctg cctttattgg cagcaacagt
    34501 tctagcaagt caagggcaaa ccaaattgac tcaggttcct attttgtctg atgtctatac
    34561 aatgaataat gttgtacgtg gtctgggagt tcgtgtgaaa tttgacgagg aaacaaagac
    34621 tatcgatatt gacgcaacag gtaaacttgg tagcgaaact ccttataagt atgtgagtca
    34681 aatgcgcgcc tctattgtta ttcttggtcc gattttggca cgcaatggtt atgctaaggt
    34741 ttctatgcca ggaggttgta caattggtag ccgtccaatt gatttgcact taaaaggttt
    34801 gcaagctatg ggggctaaaa ttaaacaacg agctggttat attgaagcga gtgcagagaa
    34861 cttgcaggga gctcatatct atcttgattt cccaagtgtt ggtgttgggg caacgcaaaa
    34921 tttgatgatg gctgcaactc tggcgcaggg tacaacggta ttagaaaatg cagcgcgaga
    34981 acctgaaatt gttgacttgg ctattttcct aaacaaaatg ggggcaaaag ttaagggggc
    35041 aggtacagag acgattacga ttactggtgt agaagaattg actggcgcag aacatcaagt
    35101 ggtgcaagat cgtatcgaag cgggtacctt tatggtggca gcggcaatga ctggtggaga
    35161 tgtcttggtc aaggacgctg tctgggagca taaccgtcca ctcatttcta agatgttgga
    35221 aatgggagtt gaggtgacgg aagaagctga agggattcga gttcgttcgc aagtggataa
    35281 gcttaaacct gtaacagtaa aaaccttgcc ccatccagga ttcccaacgg atatgcaggc
    35341 tcaatttaca gctctaatgg cagttgcaaa gggtgaatct accatgattg aaacggtatt
    35401 tgaaaatcgt ttccagcatt tggaagaaat gcgtcggatg gatttgcatt cggaaatttt
    35461 acgagataca gctatcatca cgggaggact tgctctgcaa ggtgctcagg ttatgtctac
    35521 tgatttacga gcaagtgccg ctttgattct ggctggtttg gttgctgaag gtgaaacgat
    35581 tgtaggtaaa ttaacgcact tagatcgagg ttactatcgt tttcatgaga aattagctgc
    35641 tttaggtgca acgattgaac gtgtgagtgg agaagatgaa aatggataag gataatctta
    35701 gctatgttgg taaaaattta atcttagtag cagttgtttt gttgattgct attttggtgt
    35761 ttgttttagg attaatggtt ggttacggtg tcataggaga tggagacaat gtatttgcgg
    35821 tgttgtcacc agctaagtgg caagaactaa tcggtaaatt tacaggaaag taggctggac
    35881 ctgctttttt gatggagaga aaatggcgaa gaaaaatgta agaaaacaag caatgagtct
    35941 gctcagctta ttggctacga ttgttgtcgt agcgggtggc tatcttttaa gtgataacaa
    36001 gagcagtaac gttgctgatt attcctatta tgtcaatcag tctaaggcaa gtgaaggcac
    36061 acctagtcag gaactggcta gttcggtatt gacagaagat gtaaaaaagc aactggggaa
    36121 ttcgattgaa tggaatggcg ctggagcatt tattattaac ggtaatcaaa cagatttaga
    36181 tgcatcggtt gccagtgtac cttatggtga taataagaca aagacagtcc agggagaaac
    36241 ggttccaaca gtagccaatg cgcttttagc taaatctaca cgtcagtata aggatcgtga
    36301 agaaacagga aatggtcgaa cttcttggac acctgcgggt tggcatcaga tccatgattt
    36361 aactggagaa tatgaccatg cagtggaccg tggtcacctc ctaggttatg ctttgattgg
    36421 tggattgtct ggcttcgatg cttcaaccag caatcctaag aatattgcag ttcaaacagc
    36481 ttggtccaac caagctaacg accaaacttc aactgggcag aattattttg aaagtcagat
    36541 tcggcgggcc ttggataaaa ataagcgtat ccgctatcgt gtgaccttgg tgtatgctga
    36601 gagtgaagac cttgttcctg tcggaagcca tctagaggca aaagcagcag atggtagcct
    36661 agagttcaat gtgttcgttc caaatgtgca gagtggtctc agtatcaact atcatagtgg
    36721 ggaaattgca gtaaatcaat aaatagtcag atgaattctt cctcgctaaa tcaaatatat
    36781 cagacgattt tcacgtgaaa tcgtcttttt ttgcgtataa ttaaaaatat ttacaaaaac
    36841 agttgacaaa tcatctgatg aatattagaa tatggttaaa gaaaacgctt acgactttgt
    36901 cgtagaaaac atttaggagg aatttccaat ggaaaaacat gttgagcaac gtgtcgaaga
    36961 cgttacttat aacaaagcca agttatggca aatcatatta tttgcgatga acaattcatc
    37021 gacgaatatt tacttggttg cttttacttt cgtgacttat ttatcgacag gtgtgcttgg
    37081 tcttgcagcc ctgtttgtta gtcaactcat gggctatatt cgtatctttg atggtttcat
    37141 tgatcctaca atcggagtta tcattgataa aacggatact aagtttggta aatatcgtcc
    37201 tatcttggtt ttggggaata tcattacagc agcttctctt tttatgatgt taggtttaag
    37261 tggagttaat gagagtctcc gtcttcctct attcatcctt gttttgatta tccataaaat
    37321 tggatattca cttcaacaga cgattacaaa agcaggacaa gttgcgctta ccaatgatcc
    37381 aaagcaacgt ccaatcttta atattgttga tggtattgcg acaactgtgt tgatgactgg
    37441 tagtcaggtt gttgtgtcag ggacattggt tccgaaacat ggtggtttca ctccaagttt
    37501 cttccaggaa ttgttcctcg gtgtagtagt aatttcagct gttctgggta ttcttgctat
    37561 tatcggtatt tgggagaaag acaataagaa atactttggt cttggtgaga agacacaaga
    37621 aactaagttg aaagactact ggaaagttat caaaggaaac aaacctttgc aggttctttc
    37681 aatctctgca gcttttgtaa aatttgttgc tcaactgttt ggagattccg ttatcggggt
    37741 tatgctgtat ggtattatct tcggtgacta tgctctttct ggaaaaatgt ctatgctttt
    37801 gattttgcca ggtattcttg taacagttgg tgctgcgcaa ttggctcgtc gtaaaggttt
    37861 acgttttgcc tatattacag cgcttcaact tgggattgct ggtcttgtga tcttggcagc
    37921 gatgctcttt tcagctaaac caggtatgtt ggatttgaca aattggaatc tctacacaat
    37981 cggcttcttt gttgtttata tcgttgctcg ctacgcatct agtgctccat ctggtttggt
    38041 attgaccatg ggtgcggata ttacagacta tgaaacttca gtatctggtc gttacttgtc
    38101 aggtatgatt gggactatct tctcattgac agactcaatt gcttcttcat ttgccccaat
    38161 ggttgttgga tttgtattag caggtatcgg ttttggtaaa gagtacccaa ccgttgaaac
    38221 ggcattctct acagatttga gaatggcact gattgtccta ttgacagtga ttccagccgc
    38281 ggtattgagc gtatccttgt tcttgatgaa attctacaaa ctagaccgtg aagaaatggc
    38341 acgtattcaa gagaaaatcc atgttatgaa agcggcaaaa gatgaggaac gtgtgcgtgc
    38401 aatcgcgaaa aatgttccat tatctgatat ggattatgtt gatgtaactc aatataaggt
    38461 tgatcaatac gacgataaat aatacaataa cgacctagtt ggaggagtcc agctgggtct
    38521 ttcatctagg aggcggtatg aaaaaaatct tagcatttgg agaagtttta ttgcggctat
    38581 ctcctccgca gtatcaaact ttgacacagg ctaccagttt agattgtcag tttggtggtt
    38641 cggagttgaa tgttttagcc agcttggctc agctggggca ttcagttgag ttggtgacgg
    38701 cactgcctaa gaatgaagtg ggaaaaatgg cagaacactt tctctttgca cggcagattg
    38761 gacagaaata tgtgattcga aacgagggaa ggcttggact ttattactat caaaaaggtt
    38821 tttcgctcag gcccagtcag gtgacctacg accgtgatta ttctgctttc agcttatcca
    38881 aggaagaaga ttatgatttg gaaggcatgt ttgacggtgt cgattggttt catgtcagtg
    38941 gtataacggt cgctttgaat ccagatattt atcgactggc ttttcgtttg atggaaatgg
    39001 caaagggtca gggtgttaag gtttcctttg acttgaatta tcgagaaagt ttgtggtctt
    39061 cttttgaaga agcgagggag aaattatctc cttttgtagg tttagcagat atttgctttg
    39121 gtctagagcc tattcaactc ttcaatgaat ctggaaaaga tctgaaggat gaattggggt
    39181 tgaagcgccc ttatgaaaat aaagaagtct tgttagcggt ggttaaaggt ctggcggaga
    39241 cttattcttt gcagggtctt gcttttaccc agagggaaat gacctacaat aatgaatatt
    39301 tgctgaaagc atatttgtat caagatggta ttctttatga aacggaaaaa gaatccattc
    39361 aagttctgga ccgagttggg acaggggatg cctttacagc aggaattatt ttgggctatt
    39421 tgcaggaaaa atctccacgg gaaatactcg acttaggtat ggctagtttt caattcaaac
    39481 ataccattga aggggacatt aatgtcatca gtcaatcaga tattgatttg ctattgaaaa
    39541 aagggtcacg tgaaataaaa cgatagaaag aaggaaaaag tatgttgtat ccaatacaga
    39601 caaaaacacg ctcagtttat tctttaaatg gaatatggaa atttaagcaa gggaacaatg
    39661 atgttcatac tctgttagat acagatgaag taatggttgt accgagttct tttaatgatc
    39721 ttgttgttga taaggacaaa cgtcgttttg tcggagataa ttggtatgaa cttatggtag
    39781 ctctccctgt cgtatcagct gatgaagagt tggttgtgcg ctttggttcg gtgacacatc
    39841 aggccaaagt ctatgcagat ggtcagttaa tcggggagca caagggtggt ttcacacctt
    39901 ttgaatgctt gattccgtca aacttatacg acgctgatca attccgtttg acggtttgtg
    39961 ctaataatga gttgaattac acaaccctcc cagtagggaa ttatagcgaa gaagtggatg
    40021 agaatggtca aattgtgaag accgtcaagg aaaattttga ctttttcaac tatgctggta
    40081 tccagcggac agttcatctc tataaacgtc cccaaaatag gattgaggat attgttattc
    40141 ggacggagct aaatcaagat ttgactcagg cggtagtgaa tgttgatgtg aagacagttg
    40201 ggcagtacga ttccatccgt atgtctattt tagatcaaga gggacaggaa gtaggtcttc
    40261 ttgaaaatgg acaaatggtc attgaacatc cacgcctttg ggaggtcttg gatgcctatc
    40321 tctatactgc taaagttgaa ttatttgatg gagaaaactt gctagatacc tactcagaac
    40381 aattcggcat tcgtagtgtt gcagttgaaa atgggcagtt tttcatcaat ggaaaacctt
    40441 tctatttcaa gggatttggt aaacatgaag ataccttcat caatgggcgt ggtttcaatg
    40501 aagcagccaa tctgatggat ttgaatttgc ttaaagatat tggagcaaat tcctttagga
    40561 cctcccatta tccctattct gaggaaatga tgcggctggc agatcgtttg ggaattgtcg
    40621 tccttgatga agtgccagca gttggcctat tccagctttt caatgcagcc ttgaatttga
    40681 caggggattc tgaagaggta aaaagcacct gggaagttat gcaaaccaag gatgctcatg
    40741 aattggtgat tgatgaattg atagcacgtg acaaaaatca tccatccgta gtgatgtggg
    40801 ttgtcgccaa tgagccagca gggcatgaaa aaggcgcgcg tgcttacttt gaacctctga
    40861 ttcagcgcat gagggacaga gatccgtcta agcgtcctgt gaccttggtc aatattatga
    40921 cagcaacgcc tgataaggat gaggttatgg atttggtcga tgttgtctgt ctcaatcgtt
    40981 actatggttg gtatgttgct catggtgact tgaaggcggc agagaaaggg ctccgtcaag
    41041 agttggagac ttggcaaaaa ctttatcctg acaagccaat tttgattacg gaatatggtg
    41101 cagatactct tcaaggtttg cattcgatgt gggatattcc ttatacagag gaatttcagg
    41161 tggattacta tgatatgaac catcgcgtct ttgattctat tcctaattta gtgggtgaac
    41221 aagtctggaa ttttgctgat tttgaaacca cggttggtat cattcgcatt caaggaaatc
    41281 acaaaggatt attctcaaga aatagacaac ccaagcaaat tgtaagagaa atcaagaaac
    41341 gttggactgc cattccaaac taccattata agaggaaatg agctataata gtagcaaata
    41401 gaaagttgta ttggaggacc aagcacatgg cgaaaccctt ggtagaaaaa gcatcggaac
    41461 gcttattgga attgattttg gaaagaggat atgaagttgg tgataagctt ccaaatgagt
    41521 atgaattggc ccaagattta gaagttggac gttcgactgt tcgtgaagct gttcgaagtt
    41581 tggctactcg aaatattctg gaggtgcgcc aagggtcggg aacctatatc agctctaaaa
    41641 aaggagtatc agaggatccc ctaggttttt ctttggtaag agaccgtgtg aagttgacga
    41701 cagatttgtt tgaagtgcgg tatttgcttg aaccgagaat tgcggagcgt gtcgctcagt
    41761 ttgcctcgga cgaagacatt gctcgactag aagaaattgt ccttgctatt gaagaggccg
    41821 ttgctgctgg agacacgaag catttggagt tggatgtgaa attccactgt atgctggctg
    41881 agatgagtgg gaatctggct atgacaagtc tgatgcccgt cattaatcaa tctattcatc
    41941 tgatcaatga aaattattca aatcgtcaga tgaaatcgga tagtttacag gcccacagga
    42001 acatcttaat ggctatcaag caacgccatc ctgttgcagc atatgatagc atgttagcac
    42061 atattatatc agtaagacag gttgttttaa gcgaatggtt cgataaagat atgaccttgc
    42121 atggtctcaa cgatcataaa tagaaataaa aaatagacag cccagaagaa ttcttctggg
    42181 tattttttgc ttttttattg acaaatcatc tgatgaattg tatactatag ctatgaatga
    42241 caacgctttc taattggagg tcaagatgtt aaagcaatta aaagaaaatt acttttttgc
    42301 agtgattcgc ggaaaagatg aagaagatgc gaaagaaatc gctcgccatg cgattttggg
    42361 aggtattcga aatattgaaa ttacattttc gactccaaat gcagcgacag ttatcaaaga
    42421 attacaagag gagttctcat acgattcttc cgtagtcatc ggggcgggaa cagtgatgaa
    42481 tcttaagctg gcacaagcgg ccattgatgc aggtgctagt ttcctggtta gtcctcattt
    42541 tgataaagaa attcaagact tggcgcaaga ggctgaggta ttctacttcc caggctgtgc
    42601 aactgcaaca gagattgtca cagctagtca ggcgggatgt cccattatca aacttttccc
    42661 tggtggtgtc ttggggcctg gatttatcaa ggatattcac ggaccagtgc cccaagtaga
    42721 tttgatgcct tctggtggcg tatcagttga aaatgttgca gattggaaaa aggcaggtgc
    42781 ttgtgctgtt ggagtcggtt cggctctggc aagtcgagtg caggctgaag gctatgaaag
    42841 tgtaacgacg attgctcgca gttttgttgc agctttggag ggatgaaatg agttttaatg
    42901 acaaaaactt tatgctaaaa aacgagcctg ctaaggagct ttacgggaaa atagcagagc
    42961 ttcctatcta tgatttccat tgtcacttgg atcctaagga aatttttgaa gataaggttt
    43021 atgaagatat tgttgatctg tggctggggg gtgaccatta taagtggcgc ttgatgcggg
    43081 ctaatggtat ctcagaagaa gaaatcacag gttcagcaag taaactagat aagtttaaag
    43141 cctgggcaag aaccttggaa cgggctttcg gaaatcctct ctatcattgg agtcacttgg
    43201 aattgcgaca ggttttcggt gttgaggaat tgttgaccga agaaaatgca gagcgactct
    43261 atcatcagct caatgcttat ttgcaagaac atcgagttag tccacgtaag ctgattgcgg
    43321 atgctagggt gacctttatt gggacaactg accatccact agatagcttg gaatggcata
    43381 agcgcttggc tgaggactcg accatagata ctgtagtggc accgactttc cgcccagatg
    43441 aagcctttat cgagcatcga aatttcaatc tatttatgaa acgcttggag gaagtaacag
    43501 gggtagcggt aagagacttt gcaagttttg tagaggcact tggtcagcgc gtttcctatt
    43561 ttgctcaaca cggttgccga gcaagtgaca tcagttttac ggctatttcc tatgaagagg
    43621 ctaccctcga agagttggat gaaattttac tagcaagact tgcaggtaaa gaggctggcc
    43681 aatccagtat caataaatgg cagactgcta tctttagaga attgtgccga ttgtacaaga
    43741 aacatggctt tgtgacacaa gttcactttg gtgccttacg gaacaaccat acaggactgt
    43801 ttgaaaaact tggagcagat gtaggtgtgg attcgattgg agaccagaca tgtctgactg
    43861 tgaatttgaa tcgtttgtta gataacttgg tcaaggaaaa tgcccttcca aaaatgattt
    43921 ggtacaatct caatccaagt tacaacattg ctctggccaa tactttggcg aatttccagt
    43981 ccaatgagga agggcttcgt agtcagttac aatttggtgc tggttggtgg ttcaatgaca
    44041 ccaagttggg catgattgac cagatgaatg cctatgcaga acagggtatg cttgctaatt
    44101 ttgttggtat gttgacagat tcgcgtagct ttttatctta tcaacggcat gattatttca
    44161 gacgcatttt agcgacctat gttggtcaat ggatagttga tgaggaagtg ccagaagatt
    44221 acaatcgttt agggcaattt gtagaagcca tatcctatta caatgcaaaa gagttttttg
    44281 aacaatagaa ggagagtaaa tcatgaaaat gtcatttcgc tggtatggga aaaaggatcc
    44341 tgtgacactg gaagagatta aggcaattcc tggtatgcag ggaatcgtaa cagctgtcta
    44401 tgatgtgcca gttggtcaag cttggcccct tgaaaacatt ctagagctga aaaaaatggt
    44461 cgaagaggca ggacttgaaa ttacggttat tgaatccatt cctgttcatg aagacatcaa
    44521 acaagggaag ccaaatcgtg atgagttaat tgaaaactat aaaacttcaa tccgtaatgt
    44581 aggggctgca ggtattccag ttgtatgcta caatttcatg cctgtgtttg actggacccg
    44641 ttcagacttg caccacccac ttccagatgg ttcaacttct ttagctttcc ttaaatctga
    44701 tttagcaggt gttgatcctg tggcggatga tttgaactta ccaggttggg attcgtccta
    44761 ttccaaggaa gaaatgaagg cgattattga aaattatcgt cagaatatct ctgaagaaga
    44821 tttatgggcg aatttggagt attttatcaa agcgattcta ccaacggctg aggaagctgg
    44881 cgtcaaaatg gccattcatc ctgatgaccc tccgtatgga attttcggat tgccccgtat
    44941 tattactggt caagaagccg ttgaacgatt tttgaatctc tatgattcag agcacaatgg
    45001 aattaccatg tgtgtcggat cctatgcttc tgatccgaaa aatgatgtcc ttgccatgac
    45061 ggaatatgct ctcaaacgca atcgtatcaa ttttatgcat acgcgcaatg tgacagcagg
    45121 ggcttggggc ttccaagaaa cagcacatct gtcacaggct ggtgacattg acatgaacgc
    45181 tgttgttaaa ttattggtag attatgactg gactggtgcc ttgcgtccag accacggtcg
    45241 tcgtatctgg ggagaccaga ccaagacacc tggttatggt ctgtatgacc gagcccttgg
    45301 ggcaacctac tttaacggtc tctatgaagc caatatgcga gcagcaggca agacaccaga
    45361 ttttggaatt acagtcaaaa cagtcggaaa taaggagagc tagtatgaca cgtgttattg
    45421 aatttaagga caaggttgtt gttattacag gagcaggtgg cgttctttgt ggctatttgg
    45481 caaaagaatt cgctaaggca ggtgcaaagg ttgcgctttt ggacttaaat gaagctgcgg
    45541 ctcaggtctt cgttgatgag attgcagcag caggaggcgt agcaaaagcc tataaatcaa
    45601 acgttctttc taaggaaaat ttggaaggag tccgccagca agtcctagca gattttggtc
    45661 ctgtagatat tttaatcaat ggtgctggag gcaataatcc aaaagcgaca acggacaatg
    45721 aattccatga gattggacta ccagctgata ccaaaacttt cttcgacttg gatgaggcag
    45781 gtatcaactt tgtattcaat ttgaactatt tgggaacctt attgccaaca caggtctttg
    45841 cacaggatat gattggtcgc tcgggagcaa atattatcaa tatttccagc atgaatgcct
    45901 ttactccttt gacaaaaatt ccagcctatt caggtgccaa ggctgctatt agtaatttta
    45961 cccaatggtt ggcagtccat ttttcaaaag ttggcatccg ttgtaatgcg attgcgcctg
    46021 gattcttggt taccaaccaa aaccgtggct tgctatttga cgagtcaggt caaccgaccg
    46081 cacgtgccaa caagattttg accaatacac caatgggacg gtttggtgaa gcggaagagc
    46141 tagtcggcgg agtcttcttc ttggcagatg agaatttggc aagttttgtc aacggtgttg
    46201 ttttaccgat tgacggtggc ttctcagcct attcaggagt ttgatgatga aaacggatta
    46261 cgtattttgt gtggactctg atggctgcgc catggacacc atgacctaca aacataaact
    46321 ctttttcggc cccttggctg cagatgtttt tgatgtcaaa gatagagaac cgtttttagc
    46381 tgaatggaat cgagtcaatc tgtactctcg agaacgtggc attaaccgtt ttgtaggctt
    46441 agttaaaggc ctagactttg caggtgtgac tggtatagat aacttgaaaa attgggtcgc
    46501 gaccacagat tctttgtcaa atgcttctct ggaaagattg atagaggaga cgccatcaaa
    46561 ggacttggaa ttagctttag aatggtccac tcaagtcaat caagccatta agcattatag
    46621 cggtcctgtt ttagccttca tcggcgttca caagggcttg gaaaaattga gccagttagg
    46681 aaaagtctac gtggtttctt ctgctaataa ggaagcagta gaagaagagt ggacagacca
    46741 aggtttgctg gactttgtta cagaattgta ctgtcaagac cgtggcaaga aagaagatgt
    46801 cattaaaata ttaatagagg aagggtattg tcctgataag attatgatga ttggtgactc
    46861 gcctggtgat ttgaaggcgg cggaattgaa cggtgtccat ttctatccta ttttagtagg
    46921 acaagagatg caatcttggg cggatttgac agaaacgatt gcagatgaat ttgctcatca
    46981 agcattcaca ggtgagaaag aaacagaatt gatacaggca ttttggaata atttagacga
    47041 ctagtagaga agggctagag gcgcgatgcc ctctagcttt tttggaggca aacatgacac
    47101 atttagtaga tttacggaag aaaccttaca gccttgatga agaggctatt tcttggattg
    47161 aagaaaccat tgctcagatg accttggatg aaaaaatcgg tcacctcttt gtcaacatgg
    47221 ggagccagcg gacagaagaa tatctgactg gagtattaaa tgattataag attgctgctg
    47281 ttcgttacaa tcctggacct gctgcagata tttgggagca aaactatatt ttgcagacca
    47341 agtctaagat tccccttttg attgcagcca ataccgaatc tggtgggaat ggcgctgtta
    47401 cggacggaac gaaaattggg gatgaaatca agattgctgc gacagctgat ccccgctatg
    47461 cttatgaaat ggggaaagtt gcaggacttg aagcagctgc agtaggctgc aatgcctcct
    47521 ttgcaccgat tatggacctc agccgtaatt ggcgcaatcc aatcattgca aaccgaactt
    47581 ggggagctaa tgtagaccaa gtcattgaat tgtccaaaga atacatgcga ggcattatgg
    47641 aacatggcat tgtgccgttt gctaagcatt tcccaggcga tggtattgat gaacgtgacc
    47701 atcatctctc ttttgcttcg aatcccatga ccaaatctga atggatggaa acatttggac
    47761 ggatttatgg tgagatggct gacgcaggtc ttccaggtat tatggcgggg catattcatt
    47821 tgccaaatgt cgaaaaagaa atgcatccag aacgtgaatt ggatgacatg ttgccagctt
    47881 cgctgaataa aaccctcttg gatgaattgc ttcgtggaga attaggttac aatggtgcca
    47941 ttgtcacgga tgccagtcac atgatcggta tgacagcctc tatgtcgcgt cgtgagctat
    48001 tgcctacagc tattgaggca gggtgtgatc ttttcctttt cttcaatgat ccagaagaag
    48061 atttgcaatg gatgaaagaa ggattggaaa atggtctgct ttctgaggaa cgtctacatg
    48121 atgcccttcg tcgtaccttg ggcttgaaag ctaaactcgg tcttcacttg tttgaaggtc
    48181 gtcgtcagga aatcatgatg ccaaaagaag aagcactggc tttgattggt agagatgagg
    48241 cgaaacagct tgcaaaagaa gtagctgaca aggcgattac cttggtaaaa gccaaacaag
    48301 aaggaatttt ccccgtaaat ccagaacgct acaaacgtat tttattggtc gaagtggatg
    48361 gctataaggg tggttttggc gccatgatca atgctggcaa gaagagagca gccgatacgc
    48421 tgaaagaact attagaagct cgaggtcacc aagtatctat ctgggaaaat acggaggcac
    48481 ggattgccaa attaccagaa gaagaaagac cagcagcgat tgcaaatgtc tatgcttcca
    48541 agcgtccaat tgccgaaatc acagataact atgatttgat tatcaattta gttgatgtca
    48601 actcaggagg aacaactcag cgaattattt ggccagctgc caagggaacg ccagaccagc
    48661 ctttctatgt tcacgaaatt ccaaccattg ttgtctctgt tcagcaccca tttgcacttg
    48721 cagatatgcc acaggttgct acctatatca atgcctatga tggcttgcct gtcacattag
    48781 aagccttggt tgctaaacta gcaggagagt cagactttac aggagtatcg cctgtagatg
    48841 cctattgtgg cttggtagat acacagattt ggcgcggaaa ataaggagga aagaatgcct
    48901 agattggttg atttgcggaa gaaaccctat tgtttgaatg atgagcagat tacctgggta
    48961 gaagatacct tggctcatct gactgatgaa gaaaaaatcg gtcaattgtt tgttaatcta
    49021 ttcttcttcg gaggggatgc ctttagtgga aataatctga gtaatcagga attgatgaac
    49081 cgatatcaca taggtggtgc tcgttatatg aatggcagtc cagaacaggt acaaggactg
    49141 attaacgagt tgcaaagcta ttcaaaaata cctctcttgg ttgcagcgaa ctgtgactca
    49201 ggaggagatg gggctgtgaa aggaggaacc tatatttcct caggtgctca atcagaagcc
    49261 agtcgggatc ctcgtattcc ctacctagca ggtctcgtat ctgcgcgcga ggagactgca
    49321 ttaggtgtaa atgtcaactt tgatccttgc gtggacattg ttcagaactg gcgcaatacg
    49381 attgtcaata ccagagccta tggtacaaat gcggatgatg ttattgccta taccagtgcc
    49441 tatttaaaag gattgactgc tgaaagagat gttattcaat gtatcaagca tttcccagga
    49501 gatggtacag aagaacgtga tcagcaccta gtcctcggtg tcaatgaact ctctccagaa
    49561 gaatgggaca agagttttgg tcgtgtctat cgtcatcata ttgaagctgg tgttgagatg
    49621 attatggcgg gtcatattgc cttgccatat taccaacaac gactcaatcc aactttgaag
    49681 gatgaagaca ttttgcctgc aacactggct cctgaattga ttcaagattt attgaaagaa
    49741 aaactggaat tcaatggctt ggtcataaca gatgccagcc acatgttggg gatgacagca
    49801 gccatgcgtc gtgaagacta cgtacctgca gccattgctg cgggttgcga tatgttctta
    49861 ttcttcaata acttagaaga agattttgaa tttatgctca atggctaccg caagggagtg
    49921 attactgaag aacgccttca tgatgctctc cgtcgcattt taggtctgaa agccaaactc
    49981 aatctgcata tcaagcaagc ggaaggaacc ttactaaaat cagctgatga attggctatt
    50041 atcggctgtg aagaacactt ggcttggcaa cgtgaggcag cagacaaggc aattacgctg
    50101 ttgaaagata cacaaaaaaa tcttccaatc agtccagaaa cccatcgcag aatccgtttg
    50161 tattacctag aaggggaaaa agaagggatt atggcggcaa gtacagaagt ggttgataac
    50221 cttaaagctg ccttggaagc gcgtggttat gaagtgactg tcaatgatgg tacgactcgg
    50281 attaaaggaa aaaccttgca ataccgagaa gaagttgatt tagccttgac tgttgccaat
    50341 gtcattggat atggagcaca gaataattac cgcatccagt ggaaaacggc tatgtccaat
    50401 gaagttcctt ggtatgtaca tgaagttccg accattttcg tttcactaaa ctatacaact
    50461 caccttcacg atgttaccat ggtcaaaact gctatcaatg cctatcatca caatataaat
    50521 actatcgaat ccttacttga taaattagaa ggaaaagatg acttctatgg tgttccaaat
    50581 gagaatgttt gggccaataa atggcaggca aaactataaa actaaactcc tttctatcct
    50641 agatagaggg agttttctag gaagatttct tgccaaactt tcttcttttt gatagaatat
    50701 tcttaattga ataggagtct gtgagactag tacacctatg gaaactgccc agggagttga
    50761 gccgtgactg taagctcaat cagtgaaagt tggtcgaatt cactcacaca aggacttaga
    50821 acctgcattc acagttcaac acttccatct aaggctagtt tttcagctac tcatcgttag
    50881 tttttttcaa aatacagtca gtattccctc aaaaaacagc cttgattagc ggaaaaccat
    50941 ctcttatgtg caagcacttc acttgtgaat ataggttcat agaactaagg tctgccttgc
    51001 agatgaaaac ggatggtacc gcgtgacaac gctccgatac gagtggtgtc atgcgttttt
    51061 tattggagga attatgtcaa acattgaaca acagttggct gaattaagcc aaacgacact
    51121 agaaaagtta aaagaaatcc agcaccaagg agaaaaagaa ctacaagacc tgcgtgttgc
    51181 ggttttgggt aaaaaagggt ctttgacgga cttattgaaa ggcttgaaag acctgtctaa
    51241 cgacatgaaa ccaatcgttg gtaaacaggt taatgaagtg cgtgatgtgc tgacaactgc
    51301 ctttgaagaa actgctcaaa aggttgcagc agcaaaaatt cagcaacaac tagcttccga
    51361 aaccattgat gtgaccttgc caggtcgtca ggttaaggtc ggaaaacgcc atgtcttgac
    51421 ccaaacttct gaggaaatcg aagatatttt cttgggcatg ggcttccaaa tcgttgacgg
    51481 atttgaagtg gaaaaagact actacaactt tgagcgcatg aatttgccaa aagaccaccc
    51541 agcgcgtgat atgcaggaca ccttctacat cacagaagaa attcttatgc ggacccatac
    51601 gtcgcctgtt caggcacgta ccatggacca acatgatttt tctaaaggtg ccttgaagat
    51661 gatttcgcca ggacgtgttt tccgtcgtga tacggatgat gcgactcaca gccaccagtt
    51721 ccaccagatc gaaggtttgg ttgtcggtga aaatgtatcc atgggcgatc tcaagggtac
    51781 gcttgaaatg attatcaaga aaatgtttgg tgaagagcgt cagattcgcc tgcgtccatc
    51841 atacttccca ttcactgagc cttctgttga ggtggatgtt tcctgcttta agtgcggtgg
    51901 tgacggctgt aatgtatgta agaaaacagg ctggattgaa atcctcggtg ccggcatggt
    51961 ccacccgcaa gtattggaaa tgagtggcat tgactcaacc aagtactctg gctttgcctt
    52021 cggtcttggt caggaacgta ttgccatgct tcgctacggt atcaacgata ttcgtggatt
    52081 ctatcaaggc gatgtacgat tctcggagca gttttgatgt gagagctgtt tctgatactg
    52141 aaagtatcta gaaacaggac ggtagccgct atggcggtct acctactccc ctcactaggt
    52201 tcaaggttga aaaaaatgtc gttttcaacc ttagaacgtc gtaatctcgc actatttaag
    52261 gtgtgggaat cgctgctctg cgactttcct agctacctta ctaggctttc aagcgaaaaa
    52321 tatcgtttcg cttgaaatac cgtcgtaatc tcgcactatt ttaaggtgtg gaaatagctg
    52381 ctctgcgact ttcctagcta ccttactagg atttcaagct aaaaatatcg ttttgcttga
    52441 aatccgtcgt aatttaggaa gtttgttagg tgttcagtac gagaaacggt agccgctatg
    52501 gcggtctacc tactcccctc actaggttca aggttgaaaa aaatgttgtt ttcaaccttg
    52561 aaccgtcgta acaccgctgg agtaggtgaa cggaaatcgc tattgagcga ctttcctagc
    52621 ccagttagaa atgagaggca atgagatgca gattgatcct atatgtttgg aagaggtttc
    52681 tgttttgcag gagctggcta agcagacttt tgcagagacc ttcgctcacg ataatgaccc
    52741 gaaggaactg gaggcattct ttgaggagtc ttattccttg gaggtcttga ctagtgagtt
    52801 atcggatccc aactgccagc actattttct aagaatagat catcagatag ctggttatct
    52861 taagcttaac cagggtcctg cccagacgga gcaggagttg gaaaatgcct ttgaaatcca
    52921 gcgtatctat ctcttgcagg cttttcaagg tcgcggtctt gggaaggtct tgtttgaatt
    52981 tgctttggaa aaagcagagc agtctagttg tgactgggtc tggctgggag tttgggaaca
    53041 taattacaag gctcagaaat tctattccaa atacggattt gaaaaatttg gccagcatga
    53101 atttgcggtc ggtgataaga ttgatgtgga ttggctactg aaacggcctt tgcaccagaa
    53161 aggataatat gttagtaagt tacaaatggt taaaagaact ggttgacttt gatgcgacaa
    53221 gtcatgactt gtctgaaaaa atgtcaacaa caggaattga agtagaaggg gttgagcaaa
    53281 agagtgctgg cctgtctaag ttggttgtcg gtcaggttgt gtctgctgag ccaattccag
    53341 atacccacct caatacatgc caggtcaatg tgggtgacga aatcacgcaa atcgtttgcg
    53401 gtgctccgaa tgtcaagcct gggatcaagg tgatcgtcgc tctgccagga gctcgtatcg
    53461 ctggcaacta caagatcaag aaaggcaaaa tccgcggtgt tgagtccttg gggatgctct
    53521 gctcattgag cgaaatcggc gtgtccgact cagttgtgcc aaaggtctat gcggatggaa
    53581 tctaccacct gccagaagat gcagtcgtgg gtgagccagt cttttcatac ctggacctcg
    53641 acgacgagat tatcgagctg tccatcacac caaaccgtgc cgatgctctc tctatgcgtg
    53701 gcgtggctca cgaagtggcg gctatctatg acagcaaggt caacttcaag ccagtggtct
    53761 tggaagaaag cgacaagaag gccagcgatg tgattgaagt ggctattgag tcagacaagg
    53821 tggctgccta cacggctcgt gtcattgaaa atgtgaccat cgcaccgagc ccgcagtggt
    53881 tgcaaaacct gctcatgaac gcaggtattc gcccgctcaa caatgtggtg gacatcacca
    53941 actacatctt gctctacttc ggtcagccaa tgcacgcctt tgactttgac aagtttgatg
    54001 gcaagaaaat cgtggctcgt caggcaaaag agggtgagaa cttggtaact cttgacggcg
    54061 aagaacgtga cttgattacc gatgacatcg tcatttccgt tggggacaag gcggttgccc
    54121 tcggtggagt catgggtggt gctaatacag aaattgacaa caactctaag acggttgtgc
    54181 tggaagctgc cctctttgat ggcaagtcta tccgcaagac ctcaggtcgc ctcaaccttc
    54241 gctctgagtc ttcttctcga tttgaaaagg gcatcaacgt agcgaccttg acggaggcta
    54301 tggatacagc ggctgctatg attgcggaac tggctggcgg tcaggtcttg tctggcatcg
    54361 tttctgcggg cagcgtggat acttcggatg tcccagtcac agcgaccatt gattacgtca
    54421 accgctcgct cgggaccaat ctggactatg cccaaatcgc agatattttc cgtcgtttag
    54481 gtatggaaac aactggcgat gcaagtcagt ttacagtagc agtaccgcgt cgtcgttggg
    54541 acattcgcat tccagcagac ttggtagagg aaatcgctcg tatctatggt tacaataatc
    54601 tgccgaccac tcttccgaaa gaggacggaa cagcaggtga gttgaccctg acccagaaca
    54661 tccgtcgtca agttcgtagc ctggcagaag gtgcaggtct gacagaaatc atttcttacg
    54721 ctctgacaac gcctgaaaag gctgtggagt ttgcggctcg tccaaccaca gtcaccgaac
    54781 tcatgtggcc tatgactgtt gatcgctctg ccctccgtca gaacatggtg tctggtatgt
    54841 tagacacagt ggcctacaat gtggctcgta agaacaagaa cttggctctt tacgaaatcg
    54901 gtaaaatctt tgaacagtct ggcaatccaa aagaggacct gccacaagaa atcaataagt
    54961 ttgcccttgt cttgactggt ttggtagctg agaaagactt ccagacacca gctgtaccag
    55021 ttgatttctt ccatgccaaa gggattttgg aagcaatctt tggtcattat aagttggctg
    55081 ttgactttgt ggcgactagt gagattgctg ccttgcaccc tggtcgtaca gctgaaatcc
    55141 atttgaatgg ggctgtgatc ggctttgtcg gtcaggtaca tcctcaaacg gccaaggact
    55201 acggtattcc tgagacctat gtggctgaaa tcaatctgga tgcgattgaa gaagctctcc
    55261 agcctgctca gccatttacc gaaatctcta aattcccagc ggttagccgt gatattgccc
    55321 tgctcttgag cagtgatgtt aaacatcagg atgtcctgga tgccattgct ggtgctggcg
    55381 tgaaacgctt gactaaagtg accctctttg atgtctatgc gggtagcaat attgaggctg
    55441 gtaagaagtc tatggcttat aacctgacct tccaaaatcc tgcggatagc ctgacggatg
    55501 aggaagtggc taagtatatg gagaaaatta gtaaggcttt ggttgagctt ggagctgaaa
    55561 tccgttaaca tgtaaatctt gtgaaatcct agtcagcggg ctaggatttt ttggagggaa
    55621 gtatgaacac tcaagatatt caagaaaaat tttttcgaga aggcaagctt atcgttattc
    55681 ctaaaaaatt aaaaagtaag caggtcttgt ttgcctattt acaagaagag ttggctaaga
    55741 aaggctcggc attcacagaa aaagaagtta atgcctttct agctgagatt tatgatgact
    55801 atgctatatt gagacgctac ttggtggatt atggttacct atcgcgtgac cagtatggtt
    55861 tggaatacag aatagaagaa aagagatgat tgcggtcatc tcttttgtct tacaaaataa
    55921 agggttcaat cttcaactct atctggttgc catacttggc tagtaaatcg tctgccaggg
    55981 gatgatagag ttctcgtagt tgttcgatct tgtcagggaa ttgtttcccg atatagtcaa
    56041 acttgcattc gatggttaag aaaagttggt ggaagagttc gttgatctcg ttcaagcggg
    56101 caatcatttc ttcgtcttct ttgagaaaat caggaatttc tgagcgattg ttgacaaaac
    56161 cgtcgatgag ttttacgggg aaagagccgt attctaggac aaattcgtac atgggtttct
    56221 ccttgcctta ctattgatag gactattgta gcatagatta gaatgtgaca aaagaatttc
    56281 aactttattg cagtttaagt tttgtttaat tttattttca gtttcattta aagctaagtc
    56341 ggtagactat atcttgtaaa caagacaggg acctgtaaaa atttaggata gaagaggcac
    56401 aataatgaaa acaagaatcg tacaaaagca gtttaagcgt aaagagacta aatatatcgt
    56461 tgataaaaaa acttttgaac ttttagaaaa ggaacttcgt cagtatatga tggcagatga
    56521 gtttgcgaca tcaacgatta ccaatatcta ctttgataat gaagattttg atatgattca
    56581 agattcactt gccaaaaaga atggacgtga gaaaattcgt atgcgtgtct atgatgcgat
    56641 accatctgaa tcgagtcaag cctttcttga aattaagaaa aaagaaaata aaattggcta
    56701 taaataccgt ttaacttcaa accctgtttc tgtagcaaat tatatcgaaa atggagtgat
    56761 tgattccaca atcaaggatg ataaggtaaa ctcagaactc gaaatgttga gagaacgcta
    56821 cgggactatt aagccgaaga tgtatatcta ctacgatcgc gtatcctata aaggaatcga
    56881 agacaaaaaa gttcgtctca caatcgataa gaacttactt tatcgagact acgatgtgga
    56941 tgcaatggaa ggtaaatttg gtaagaacct attagaccca accaaagtaa tcatggaagt
    57001 aaaagttcca gaagaacgac cagactggtt ggtagccctg ctcgaaaaat accaaatcga
    57061 aaagcaatca ttttcaaaat atggcaatgc ctataagctt gcccacaaca tcactggaga
    57121 ggaagtaagt caacatgcag ttgtttaata gtatcttttc aactgccaat agcaatatta
    57181 ccttagcgca gatgtccatg gtttttggag ttagtctatc catgggggta ctcttagcgg
    57241 ctgtttataa atataaatct aattatacaa aggaatttgt catcacacta agtttaatgc
    57301 cggcattgat tgctgttatt attgcattag ttaatggtaa cttaggagcg ggtgttgcag
    57361 tagcagggac tttcagtcta attaaattcc gctcagcagc aggttcttca aaggaaatgc
    57421 ttgcagtcct cttagcgatg gcaatcggtc tagcgacagg gatgggcttc ctaggtttgg
    57481 ctgtcttcat gacggtcatc ctatctgctt gtatcttgat ttttgaaaat atcagctttg
    57541 cccaagtcaa tcaaaatcgt cgtcatctct tggtgacagt gccaatgaat tttgactacg
    57601 atcaattttt tgaaaatcaa tttggtaaat cttgcaagca gtctgattta ttatccctta
    57661 aatacaaaaa gaaaaaagaa gcacttgttt tagaatatca agtccttctt gataagaaaa
    57721 ttacagataa acgtttggta gatacagtat tgtcagccgg tcccttggat gtcgtattaa
    57781 acaagcaaat gccgaagaag aagtatttgt aatatcaaag aagccctgga tttccaaggc
    57841 ttttttcgtt ttttattagc ttattcttca taaaaatcaa cactgtaatc aagcagtttg
    57901 cccccatcat agcggaagca agcggaaaaa aatggtttgt cctctaatag ccattcttga
    57961 aaatgccggt cgccctccca agttggtttg gacaagactt ggtcataggg aacccactct
    58021 aagtcacctt cattgcagtc aataagactt ccttcaaaac cagttatctt aaagacatag
    58081 gtgtaccagt ctgtgtttgg agtaaagtct gggaaggtta tcactccttt tagagcatgc
    58141 tttgttacag taagcccagt ttcctcaaag atttctcgaa tggcacaagc ctgtggcgtt
    58201 tcgccgggtt ctagtttgcc accaactcca atccattttc cttgatggac atcgttttct
    58261 ttcttgtttc gatgaaggag aagaaattcc ttaccattat caatgtagca aatcgtagcg
    58321 agttggacgg gtttcttggt catagaggct ccttgtagtt gatttatcct attataccac
    58381 aaaggtggga gtagggacac ctgcttgttg ctctgatagg ggacagtttt aggattagca
    58441 tactgaaagc ccttttctga aaacagtttt tgaggtataa taagaacatc aaactattta
    58501 ggatgcacta tgtcaaaaca agaacaaatc gaacaaacaa tttatcaatt attagaatta
    58561 ttgggtgagg acccgaatcg tgaaggttta ttagatacgc ctaaacgggt cgctaagatg
    58621 tatttagaaa tgtttaacgg cctagaagag gatcccaaag accaatttac agctgtcttt
    58681 tcagaaggtc atgaagaggt tgttttggtc aaggatattc cttttcattc tatgtgtgaa
    58741 caccatttgg taccctttta tggcattgcc catgtagctt atattccgag taaggggcgt
    58801 gtgacaggtc ttagcaaatt ggcgcgtgca gtggaggtag caagtcgtcg ccctcaattg
    58861 caggaacgct tgacccatca ggttgcccat gcccttcaag atgcccttga accagaaggt
    58921 gtttttgtca tggtcgaagc agagcacatg tgcatgagca tgcgtggtat tcgcaagcca
    58981 ggtagtaaga cagtgaccac agttgctctt ggcaaataca aggaagatgc catcttgcgc
    59041 cgtgaactct tgtccatgat tcacaataaa taggaggttc tatgtcaatc gaacagttag
    59101 cgaatcaagt aaccattatg gggattctca atgtgacgcc agattcattt tcagatggtg
    59161 gtcactataa tgaggtggag tctgccttgg tacaagtaga aaagttgttg gctgagggtg
    59221 ctaccgtcat tgatgttggt ggtgagtcca ctcggccagg tgcaacattt gtgtctgaag
    59281 aagacgagat tgctcgcgtg gttccgatta tccgcgctat caaagaaaac tatgactgtc
    59341 ttgtcagcgt cgatacctat aaaacaggaa ctgcacgtgc agccttggaa gcaggcgcag
    59401 atattttaaa tgatgtctgg gcaggacttt atgatggaga tatgttggcc ctggctgcgg
    59461 agtacaaggt accaatcatt cttatgcaca accagaaaga cgaaagctat gcggatgttg
    59521 ttggagaggt caaaaacttt ctagctgagc gtgcccaagc agcacttgat gctggtgttt
    59581 ccgcagacca gatttggttg gatccaggat ttggcttttc aaagaatgtc caacataacc
    59641 ttgatctctt acaaggtttg gaacagatta ctgctcttgg ttatcccgtt ctatttggaa
    59701 tatctcgtaa gcgcgtggtg gattatctgc ttgggggcaa tagcttaccg actgaccgcg
    59761 atcaagcaac ggcagccttg tctgcctggg cagttcaaaa aggctgtaaa atggtccgtg
    59821 ttcacaatgt cgctgccaac cgtgatatcg ttaaggtttg ggaccaattg acttctggag
    59881 gtcaacatgg ataagatttc acttaataaa tgccgtttct acggctacca tggtgccttc
    59941 aaagaagaac aagttcttgg tcaaattttc acggttgact gtgatttgtt tgttgacctg
    60001 acagcagctt cacaaactga caatttagaa gacacagttc actatggtat ggtctttgaa
    60061 accattaaag cagttgtgga aggcaagccc tacatcctta tagaaaaggt agcaggtgtt
    60121 atttgtcagg agctttttga tcaatttcca aaggtggaga agattcgctt ggccatttac
    60181 aaggaaaatc cgcctattgc tggacactat gattctgtcg gaattgaatt ggagcgtgaa
    60241 cgaccatgaa gcatctagcc tatctcagta tcggagggaa catgggcaat cggcaagcct
    60301 atttacaggc agctctagac aaattagcca gtcatccagg ttgccagctt ggcttagttt
    60361 ccaatattta tgaaactcca gcttggggca agaccgacca ggctgatttc ctcaatctag
    60421 cctgtcaagt gtatactgat ttgtctgctc aagatttcct aagcgcttgc cagaacatcg
    60481 aacaagagtt ggaccgagta cggattgaaa aatggggaca acggaccatt gatttggata
    60541 ttattttttg ggatgaggag aagattcaag aagagaatct gattgtcccc catccctacg
    60601 cccatgaaag agcatttgtt ctcaaccctc ttgctgagat tgcttctgat taccgtcacc
    60661 caattttgaa aaaagtggta tcggacttgc ttgcagaact aggggaacaa aaagatataa
    60721 aaattttcaa atagttgcct ctgatactag gtttattaga ggatagaata gataaaggag
    60781 ttttatgaca attttagcaa ctttaggagt tttattggct attgtagcca ttatttattg
    60841 gacatcaaaa aatttacatg ttatcattgc tgcaccacta gctagtttaa tcatcattct
    60901 gaccaatcag atgaatgttt tggaagttat gttgggcaaa gagcagtctt atatggcggg
    60961 cttagcaggt tttttgatta ataattttgc catctttatg ttggggtcta tcttggcacg
    61021 ttatatggag gcaagtggag ctacgcagac gattgccaat agcattttga aagtaatggg
    61081 aaaagatagt ccctataagg gtttgttagc catcactttg attgcttcca ttctgaccta
    61141 tggtggtgtg agtatttttg tagttatttt taccttgctt cccctttcac gcccactctt
    61201 taaagagttg aatatcaact gggcactttt tccgattcct gtctttttgg gagcaggtac
    61261 ttatacgatg acaactcttc ctggggcacc ttccatccag aatgttattc cgacaaaagt
    61321 tttgggaact agcttgaccg cggcacccgt tatcagtttg gcagccagct tgacgctatt
    61381 agtatttggc ttgctctaca tggcctactg cctcaagaaa agtctagcaa atggagagac
    61441 gtatacagaa gaagaggatg aaactgcagt agtggtagct gataagacac caaatctatt
    61501 tttatcagtc ttgcctctat tcagtcttat tgggacgatt ttcctaatga gtaaaactcc
    61561 gaatgtccta gcaatcggat tgttggtctc tatccttcta tcagccttga ttttttatcc
    61621 atatctgcca aatcaaaaag aaattttgaa tgctgcgaca acagcatcca tagtgcctgc
    61681 ttttgcaact tctagtacag tagcatttgg gacggtgtta accttgtcag caggttttgc
    61741 agtcattcaa gaatggattc aacaaatacc aggatcgccg ctgattagtt tatctgtttc
    61801 gacagcattg gttagtggta ttatcggttc atcttctggt gcagtaggta ttgcttcaag
    61861 taatttcctt cctgcctatt tagaaatggg gattaatcca gagttgctgc atcgtgtagt
    61921 agttgttgca tcagctatct tgacagttgt gcctcagtct ggcgtgatga ttaccttcca
    61981 caatctttcg aaattaagta tgaaacgtgg actcaagtat tcatttatcc tagtgacagt
    62041 tgggcatttg ttagccttat ttgtgatttt actgttggca cctttgctat attaggattg
    62101 attaacaaac aaataaaagc cctgccaatg ggcaatatca ttcagttaaa atctctactc
    62161 aaatatttat tcgg
//
LOCUS       DAZQCJ010000004        44544 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000004 DAZQCJ010000000
VERSION     DAZQCJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44544
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.004"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(646..1845)
                     /gene="pgk"
                     /locus_tag="ACHU66_000238"
     CDS             complement(646..1845)
                     /gene="pgk"
                     /locus_tag="ACHU66_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR3388554.1"
                     /translation="MAKLTVKDVELKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYILEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQEVAFIAGATRGAELE
                     AAINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAH
                     ASNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLE
                     KADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANA
                     FAGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQA
                     GTIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPG
                     LAALTEK"
     gene            complement(2105..3115)
                     /gene="gap"
                     /locus_tag="ACHU66_000239"
     CDS             complement(2105..3115)
                     /gene="gap"
                     /locus_tag="ACHU66_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR3388555.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKDGGFEVNGKFVKVSAEREPGNIDWATDGVDIVLEATGFFAS
                     KEKAEQHIHANGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALHDAFGVQKGLMTTIHGYTGDQMVLDGPHRGGDLRRARAAAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVATLDKKVTAEEVNAAMKAAATESFGYTED
                     QIVSSDIVGISFGSLFDATQTKVIEVDGEQLVKVVSWYDNEMSYTAQLVRTLEYFAKI
                     AK"
     gene            complement(3482..5473)
                     /locus_tag="ACHU66_000240"
     CDS             complement(3482..5473)
                     /locus_tag="ACHU66_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HFR3388556.1"
                     /translation="MRKKLKFSLVAVACATFLTACGTSNSSAPKAGEATPQTDFHSYI
                     NEQWLKDTKLGAGESQIDNLSQLSEKTQEAIQEMIDQLDMDYSKLQAGSDEKKLIDFY
                     RLAADFDTRNKLGLEPIKPYLEEIKKASTMEELNKILVALYGKNIRNLLHINVTQDIK
                     DSNKNTIYVDPHKLSFPKENYDGTDEFSLKNQKAFKAYLQEVFSLAGESSKEASSKAD
                     LLFNLEKEMAAVQRPKEEANNFEAMYNEKTWDEVKALVPNLPIAELVSELGLQNAPTT
                     VVSEPDALKKLNELYQEKNLDALKALLQYRVIAHYNDYLSSNLIEAKAKYDGVATGAV
                     DTPSQEEVAQAAIDANFSDMIGKVYVKHHFSEESKANILSMVQEIKATYAERIKAVQW
                     MSEETKMRALKKLDTLVVKIGYPDQWKDYSQLAIQSKEEGGSLLSNFDMVKTLELREE
                     MGQFGKPFDKSQFGMPSHTVNAYYSPTNNEIVFPAGILQAPLYDPKASPEENLGGIGA
                     VIGHEISHAFDKAGSQFDEKGNLVNWWKPEDLEKFNKKVQQAADIYSKLEVAPGYYVN
                     GEISTGEIMADLGGLTVAIDIAQKKGYDTKKVFESYGKAWREVTTKEFAIANISDEHP
                     PAKYRVNNIVNQIDQFYTDFNVKEGDPMYVKPEDRLRVW"
     gene            complement(6455..8536)
                     /gene="fusA"
                     /locus_tag="ACHU66_000241"
     CDS             complement(6455..8536)
                     /gene="fusA"
                     /locus_tag="ACHU66_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR3388557.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHERLQANAH
                     PIQLPIGSEDEFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLEQAEEYREKLVEAV
                     AETDEELMMKYLEGEEITNEELKAAIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGVNPDTDAEEERHASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVLNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESIHVPEPVIQLMVEPKSKADQDKMGIALSKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPIVDVKAKLY
                     DGSYHDVDSSETAFKVAASLALKEAAKSAQPTILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEARGNTQIVRAYVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     DEIIKKNGGNA"
     gene            complement(8978..9448)
                     /gene="rpsG"
                     /locus_tag="ACHU66_000242"
     CDS             complement(8978..9448)
                     /gene="rpsG"
                     /locus_tag="ACHU66_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR3388558.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKLVTRLINRVMLDGKRGTAASIVY
                     GAFDQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARNRGEHTMIDRLAKEIMDAANNTGAAVKKREDTHKMAEANRAFAHFRW"
     gene            complement(9465..9878)
                     /gene="rpsL"
                     /locus_tag="ACHU66_000243"
     CDS             complement(9465..9878)
                     /gene="rpsL"
                     /locus_tag="ACHU66_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR3388559.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSRKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKG"
     gene            complement(10110..11732)
                     /gene="groL"
                     /locus_tag="ACHU66_000244"
     CDS             complement(10110..11732)
                     /gene="groL"
                     /locus_tag="ACHU66_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940029.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR3388560.1"
                     /translation="MAKEIKFAADARESMVRGVDILADTVKVTLGPKGRNVVLEKAYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPIGIRRGIEAAVATAVESLKAQASPVSNKAEIAQVAAVSSRSEKVGEYI
                     SEAMERVGTDGVITIEESRGMETELDVVEGMQFDRGYLSQYMVTDNEKMVAELENPFI
                     LITDKKISHIQDILPLLESILQTSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLDLKDATIEALGQAAKVVVDKDGTTIVEGV
                     GNAEAIANRVAVIKSQIEGTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALVNVIDSVAKLELKGDDETGRNIVLRALEEPVRQ
                     IAYNAGYEGSVIIDKLKNSELGTGFNAATGEWVNMMDAGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPAAPAMPQGMDGMGMGY"
     gene            complement(11744..12025)
                     /gene="groES"
                     /locus_tag="ACHU66_000245"
     CDS             complement(11744..12025)
                     /gene="groES"
                     /locus_tag="ACHU66_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR3388561.1"
                     /translation="MLKPLGDRIVVKIEEKEQTVGGFVLAGASQEKTKEASVLAIGQG
                     IRTLNGDLVAPAVAVGDTVLIDAHAGLEVKDGEQTVHIIREADILAIVE"
     gene            12223..12468
                     /locus_tag="ACHU66_000246"
     CDS             12223..12468
                     /locus_tag="ACHU66_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388562.1"
                     /translation="MKFFRELKTDYLESRFSVHESFAEWFLKRKLGFLGKIMFAYLLW
                     LVWLLLFSHPHYIIFFFYGVLLLSLIIMLIEWWKYRK"
     gene            complement(12582..12977)
                     /locus_tag="ACHU66_000247"
     CDS             complement(12582..12977)
                     /locus_tag="ACHU66_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR3388563.1"
                     /translation="MYNKVILIGRLTAQPELTQTPNGKNLTRVTVAVNRRFKTENGER
                     EADFLNVIFWGKLAETLVSYGNKGSLISIDGELRTRKYEKDGSNHYVTEILGQSFQLL
                     ESRAQRAMRENNAGDDLADLVLEEEELPF"
     gene            13032..13811
                     /locus_tag="ACHU66_000248"
     CDS             13032..13811
                     /locus_tag="ACHU66_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HFR3388564.1"
                     /translation="MKEFLMEKIHTSQPYTNAELAWLLENISHPDPAIRDELVYASFC
                     HIFLEGLITREQAQSLLQFSQETNHLSLESSTLKRSFTCLLYCLLLSVDNERDSVYFA
                     FLSTNDRELLFQQALDYLAIENDWSGYDEKLGWIHTAAHGADFLLATSCHDQFPAEKS
                     KEVWQTIVTCLTRQSKVFSAGEEIRLAQIPVYLLLNEKVTSKELTEWISKLDFPNQEP
                     VDYFRWLNLQHFLSSLYFQLRSHQASTEEIEQAIEGKIEPA"
     gene            complement(13845..14468)
                     /gene="ytpR"
                     /locus_tag="ACHU66_000249"
     CDS             complement(13845..14468)
                     /gene="ytpR"
                     /locus_tag="ACHU66_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFR3388565.1"
                     /translation="MIFTYNKEHVGDVLMMIVAEDKGQPVQFERKGQVARVFLEETGK
                     TVAWNIFEASSLVELAGNGQVFMTDEQVATLNAELAKEGFTESLVNDNSPKFVVGQIV
                     ELVPHPDSYHLNICQVNVGDKTVQIVAGAPNAAQGLKTIVALPGAMMPSGSLIFPGKL
                     RGEDSFGMMCSPRELALPNAPQVRGIIELDDSAVVGEALDPAKHWKG"
     gene            complement(14487..15443)
                     /locus_tag="ACHU66_000250"
     CDS             complement(14487..15443)
                     /locus_tag="ACHU66_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HFR3388566.1"
                     /translation="MKKSLGKILLAPVVLGLSLSLAPLVQAEIQTDVINEKWGKPTLV
                     YGGGLSDAQATEVNNLFGISDVNNVSRQVVVGTDMDQYLGTSGADTASLYSSVLVQKQ
                     DAGKGVVVDIKTPQNITLITETQYANAAITAGATDVLIDVAAPIQVTGESALTGVYKA
                     LAANGDTVDTARTEVAQQELTTVNEVATAHAGDEQFDSSALDKAVAEIKTALADYKKS
                     NGQVASEADINTIINDVLTQNGLENVITADEISKLVTFAKAYQETSAIDSAEVAAQLN
                     QFKQQAEQQISEAYKNLQDSGILEKIGAFFENLWKGLTGLFA"
     gene            complement(15488..15808)
                     /locus_tag="ACHU66_000251"
     CDS             complement(15488..15808)
                     /locus_tag="ACHU66_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFR3388567.1"
                     /translation="MIFPTNLEEVAGLIEDGKPTVFLFVTTWCGDCHYIKPHLSAIEE
                     AFPDFRFVQLDRDDFMPLAQEWAILGIPSLVVLENGQEIGRFVDKNRKTKEEIMNFLS
                     GLGR"
     gene            complement(15805..16089)
                     /locus_tag="ACHU66_000252"
     CDS             complement(15805..16089)
                     /locus_tag="ACHU66_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HFR3388568.1"
                     /translation="MKKKRTALLAGLLGAGVLTASAQFYRKVQEDRKKAQALQEVRDF
                     FANVGEIATVYVDETASTQDSLVGGVVMEAGQVFLFENAQGSIQYREEER"
     gene            16302..17363
                     /gene="pepA"
                     /locus_tag="ACHU66_000253"
     CDS             16302..17363
                     /gene="pepA"
                     /locus_tag="ACHU66_000253"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFR3388569.1"
                     /translation="MTLFEKIKEVTELQSLPGFEGQVRNHIRQKITPHVDRIETDGLG
                     GIFGIKDTTVENAPRILVAAHMDEVGFMVSQIKPDGTFRVVELGGWNPLVVSSQAFTL
                     QLQDGRTIPAISGSIPPHLTRGGNAPGVPAIADIIFDAGFTNYDEAWAFGVRPGDVLI
                     PKNETILTANGKNVISKAWDNRFGVLMVTELLESLSGQALPNQLIAGANVQEEVGLRG
                     AHASTTKFNPDIFLAVDCSPAGDIYGDQGKIGDGTLLRFYDPGHIMLKNMKDFLLTTA
                     EEAGVKFQYYCGKGGTDAGAAHLKNHGVPSTTIGVCARYIHSHQTLYSMDDFLEAQAF
                     LQAIVKKLDRSTVDLIKNY"
     gene            complement(17409..18665)
                     /locus_tag="ACHU66_000254"
     CDS             complement(17409..18665)
                     /locus_tag="ACHU66_000254"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFR3388570.1"
                     /translation="MNYQETRRWLSSRPASDLENGVARVNWILERLGNPQLQVPTIHF
                     VGTNGKGSTLNALQSILQSSGYTVGRFTSPSIIDFREQIVFEQEMISEKDFARIVTDL
                     QPLIEDLDQTAGLDAISEFEIVVVAMFVYFAHYQRPDILLVEAGMGGLQDATNVLAPL
                     AVVCPSIGLDHQIFLGETHAAIARHKVAVLRERVPLIYAIDLPEVEAVFEDHASQLQS
                     PTYAVGREILLEDSRAGFAVSSPLGRVEELKVLMQGRHQEVNAALAVTTAQLLSSHFP
                     KIINETISQGLSQAIWPGRLELMQPWLMIDGAHNNESIAVLTQLLEEKYADRDIEILF
                     AAINTKPVDQMLSQLSQFGPVSVTTFDDFRAVQLDDYPSGYERVQTYQEWLEQADLDN
                     PKKLYLITGSLYFITYVRKYILEEFI"
     gene            complement(18676..19272)
                     /locus_tag="ACHU66_000255"
     CDS             complement(18676..19272)
                     /locus_tag="ACHU66_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFR3388571.1"
                     /translation="MEKKIPKLTVQLLAAIAVTLALVMIVENYFSVRLSDTLQVQFTF
                     IPNAILGAIAGPVWAAVFAAISDPVFVLFSGQSMLFSFILIEAVSAFIYGWFFYRKPL
                     DTKNKADWLYVAGVVVLIRVVISFIMTPIALHFHFGTPWIALYSSRFIKAIFEIPLRI
                     VVTMLVLPSLQKIPELAKLVGIKQNSIKQQVISLLLLL"
     regulatory      complement(19317..19461)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            complement(19587..20774)
                     /locus_tag="ACHU66_000256"
     CDS             complement(19587..20774)
                     /locus_tag="ACHU66_000256"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR3388572.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVLAAGIVERIGLNDSISTV
                     KYDGKKESEVLDIPNHTVAVKILLEDLLKHNIIAAYEEITGIGHRVVAGGEIFKESAV
                     IGPKEVEQIEELSALAPLHNPAHVAAMRAFEEVLPEVLNVAVFDTAFHTTMPKHAYLY
                     PIPQKYYTDHKVRKYGAHGTSHYYVAHEAAKVLGRPIEELKLITAHIGNGVSITANYH
                     GESVDTSMGFTPLAGPMMGTRSGDIDPAIIPYLIANVEELKDAADVVNMLNKQSGLFG
                     VSGFSSDMRDIEAGIQAHNPDAVLAYNIFIDRIKKFIGQYLAVLNGADAIVFTAGMGE
                     NAPLMRNDVVEGLSWFGIELDPEKNVFGNYGDISTPESKVRVLVIPTDEELVIAREVE
                     RLK"
     gene            complement(20824..21777)
                     /locus_tag="ACHU66_000257"
     CDS             complement(20824..21777)
                     /locus_tag="ACHU66_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR3388573.1"
                     /translation="MNFEKIEQAYDLLLENVQTIQNQLGTNIYDAMIEQNAAYVANQH
                     EMDLVVNNNKTLKQLDLTKEEWRRAYQFLLIKANQTEPMQYNHQFTPDSIGFILSFLV
                     DQLMPTQKVTVLEIGSGTGNLAQTILNASQKELDYLGIEVDDLLIDLSASIADVMQAD
                     ISFAQGDAVRPQILKESQVILGDLPIGYYPDDQIASRYQVASPNEHTYAHHLLMEQSL
                     KYLEKDGFAILLAPNDLLTSPQSDLLKGWLQEQANIVAMIALPPNLFGKAAMAKSIFV
                     LQKKAARSLTPFVYPLQSLQEPEAIQKFMLNFKNWKQENAI"
     gene            complement(21834..22244)
                     /gene="comGG"
                     /locus_tag="ACHU66_000258"
     CDS             complement(21834..22244)
                     /gene="comGG"
                     /locus_tag="ACHU66_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFR3388574.1"
                     /translation="MLLKKKVKAGILLYALLMLAVFSLLLQFYLHRQEAESRLVQVAR
                     QETTAYIMAQMVLDKVEQDLQVEKIVTGAVVNEKEANLTTGSKQKENRGRVSFQEGQA
                     RYQLKGDQLLVTVELTSDGLYTYQFPLGLSSEGG"
     gene            complement(22222..22656)
                     /gene="comGF"
                     /locus_tag="ACHU66_000259"
     CDS             complement(22222..22656)
                     /gene="comGF"
                     /locus_tag="ACHU66_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFR3388575.1"
                     /translation="MLKSKIPAFTLLECLVALVILSGSLLVFEGLSKLLVQEAHYQGQ
                     TVQKEWLVFSSQLRSEWDQAELVKVENGKVYVNKGEQALAFGKSWSDDFRKTNDRGQG
                     YQPMLYQVDSAAISQENQLVRIDFSFKNGEERTFIYAFKEKS"
     gene            complement(22643..22936)
                     /gene="comGE"
                     /locus_tag="ACHU66_000260"
     CDS             complement(22643..22936)
                     /gene="comGE"
                     /locus_tag="ACHU66_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGE"
                     /protein_id="HFR3388576.1"
                     /translation="MVAIRKQKNKAYILLESLVALATLVTICSLILSAVDVGRRRQAW
                     ELEQQEVLNLAQMAVQTGQDKLALNGVTVQVQQTADKIIVFYEGKEVLSVVKK"
     gene            complement(22908..23315)
                     /gene="comGD"
                     /locus_tag="ACHU66_000261"
     CDS             complement(22908..23315)
                     /gene="comGD"
                     /locus_tag="ACHU66_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFR3388577.1"
                     /translation="MLRRKNKAFTLFESLLTLLVVSFLAISLSGTVQTVFRSVQEEIF
                     LWEFEAIYKDSQKLAASSRQAVSLSIGGQEVSNGSQIVKVPRNVEILEEKAIRFEEDG
                     GNSSLTKIRFRLSQKIVTYQLYIGSGRYKKTEE"
     gene            complement(23296..23577)
                     /gene="comGC"
                     /locus_tag="ACHU66_000262"
     CDS             complement(23296..23577)
                     /gene="comGC"
                     /locus_tag="ACHU66_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFR3388578.1"
                     /translation="MKKLVEMKVKGFTLVEMLVVLGIISLLLLLFVPNLSQQKDAIQK
                     KGDAAVVKVVESQMELYELEHDKEATVADLQAAGYITEKQAKQYAAAKK"
     gene            complement(23579..24616)
                     /gene="comGB"
                     /locus_tag="ACHU66_000263"
     CDS             complement(23579..24616)
                     /gene="comGB"
                     /locus_tag="ACHU66_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFR3388579.1"
                     /translation="MRKLIAFLQQDISVLGRQKQKKLPLARQRKVIELFNNLFASGFH
                     LGEIVDFLKRSQLLADLYTQVLSDGLLAGKPFSSLLADLRFSDAVVTQVALAEVHGNT
                     SLSLSHIQSYLENVSKVRKKLIEVATYPIILLGFLFLIMLGLKNYLLPQLEEGNAATM
                     LINHLPTIFLSLCGLSLVAVLAGMVWFRKTNKIKAFSRLAALPFFGKLIQIYLTAYYA
                     REWGSLIGQGLDLPQIVGLMQEQQSQLFREIGQDLEQSLSNGQSFHEHIKTYAFFKRE
                     LSLIVEYGQVKSKLGGELTVYAAECWEDFFSRVNRAMQLIQPLVFLFVALMVVLIYAA
                     MLLPIYQNMEL"
     gene            complement(24528..25478)
                     /gene="comGA"
                     /locus_tag="ACHU66_000264"
     CDS             complement(24528..25478)
                     /gene="comGA"
                     /locus_tag="ACHU66_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFR3388580.1"
                     /translation="MIQEKARKLIEKAVADRVSDIYLVPRGESYQVYHRIMDEREFVQ
                     DLAEEEVTAIISHFKFLAGLNVGEKRRSQQGSCDYDYGSGEISLRLSTVGDYRGKESL
                     VIRLLYDNDKELKFWFEAAERLAEEIKGRGLYLFSGPVGSGKTTLMYHLARLKFPDKQ
                     ILTIEDPVEIKQEDMLQLQLNEAIGATYDNLIKLSLRHRPDLLIIGEIRDAETARAVI
                     RASLTGATVFSTVHARSISGVYARMLELGVSPEELNNALQGIAYQRLIRGGGVVDFAK
                     GNYQNHSADQWNAQIDRLFAAGHISLRQAETEKIALGSPA"
     gene            25564..26514
                     /locus_tag="ACHU66_000265"
     CDS             25564..26514
                     /locus_tag="ACHU66_000265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFR3388581.1"
                     /translation="MKTLKKGDHIRVVSPSSSIERIGGFEANLAAKEKLEDLGFKLSF
                     SEHYFENDIFDSAPIASRVADLEVAFADESVDAILTTIGGFNCNELLPYLDFDLIARH
                     PKIFCGYSDTTALLNAIYAKTGMPTYMGPAYSSFKMDLGQQYQTEAWLKAVTQDSFDL
                     TPSPEWSSDAWYLPDAPRTFYPTEWKVYNPGQASGIAIGGNISTLNLLTGTEFAPRPD
                     EYILFLEEAEDDDYLIIARHLTTLLQAYPNPQALVFGRFPKETKMTEEILLAILDKHP
                     ILKTIPVLYDLDFAHTQPLFTITIGGQVELDTKAFSIRFS"
     gene            complement(26549..26914)
                     /locus_tag="ACHU66_000266"
     CDS             complement(26549..26914)
                     /locus_tag="ACHU66_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFR3388582.1"
                     /translation="MYQVIKMYGDFEPWWFLDGWEEDVTSRAAFDRYEDALNAFQKEW
                     VRLSEDFPMKKSKNGTMVAFWDESDQHWCEECDEYLQRYHSLMLVEARENLPAGFIKQ
                     PTQPRVRPCKLKQNNCVNE"
     gene            complement(27063..30710)
                     /gene="rpoC"
                     /locus_tag="ACHU66_000267"
     CDS             complement(27063..30710)
                     /gene="rpoC"
                     /locus_tag="ACHU66_000267"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR3388583.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERD
                     GLFDEVIFGPTKDWECSCGKYKRIRYKGITCDRCGVEVTRAKVRRERMGHIELKAPIS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLPKEIAALKEELKTASGQKRIKAVRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMVQLDGGRFAASDLNELYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIAGNVKAAKRLIE
                     RGDDRIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMVFKDADEAVMAYRNGYVHLHTRVGIATDSLDKPWKDNQKHKIMMTTVGKIL
                     FNAIMPEGLPYLQEPNNANLTEGTPDKYFLESGSDIKAAIAELPINPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIEESHERVEQ
                     IKKQFRRGMITDDERYAAVTDEWRSAKEKLEKRLVEKQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMSAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLDIRSITDGKEMIEPLEERLQGRYTKKTVKHPE
                     TGAVIIGPNQLITEDIAREIVNAGVEQVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNSDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGEVIAIEEDASTRTKKVFVKGKTGEGEYVVPFTARMKVEVGDQVARGAALTEGS
                     IQPKRLLEVRDVLAVETYLLSEVQKVYRSQGVEIGDKHIEVMVRQMLRKVRVMDPGDT
                     DLLMGTLMDISDFTDANAETVIAGGVPATARPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKRDNLLGLKENVIIGKIIPAGTGMARYRNLEPQAINEVEIIEDTVAEEL
                     AAEAELEAVTE"
     gene            complement(30888..34460)
                     /gene="rpoB"
                     /locus_tag="ACHU66_000268"
     CDS             complement(30888..34460)
                     /gene="rpoB"
                     /locus_tag="ACHU66_000268"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR3388584.1"
                     /translation="MAGHEVQYGKHRTRRSFSRIKEVLDLPNLVEIQTDSFQDFLDYG
                     LKEVFEDVLPVSNFTDTMELEFVGYELKEPKYTLEEARAHDANYSAPIYVTFRLVNKE
                     TGEIKTQEVFFGEFPIMTEMGTFIINGAERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDSKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTIEKDIHKNPADSRTDEALKEIYERLRPGEPKTAESSRSLLTARFFDPRRYDLAPVG
                     RYKINKKLNLRTRLLNQTLAEHVINGETGEIVLEAGTVLSRDVLEKVEAQFDELNLVE
                     YIPNDNAVLLEPVLLQKFKIVAPKDPERVVTVIGNANPAENVRTVTPADILAEMSYFL
                     NLAEGLGRVDDIDHLGNRRIRAVGELLANQVRIGLTRMERNLRERMSVQDNEVLTPQQ
                     IINIRPVTAAIKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVRD
                     VHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFIQTPYRKIDRATGTVTNEIVW
                     LTADEEDAYIVAQSTSPLDENNRFVDKIVMGRHQGNNQEFPADSADFMDVSPKQVVAV
                     ATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAIIAQHD
                     GKVVYADADKVEVRREDGSLDVYHISKFRRSNSGTAYNQRTLVKLGDIVEKGDFIADG
                     PSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDTK
                     LGPEEITREIPNVGEDALRNLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERLL
                     HAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRANGDELQSGVNMFVRVYIAQKRKI
                     KVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHLG
                     MAARNLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGVM
                     YMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVLQ
                     EILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDNNE
                     VELRDLDEGEDDDIIHVDDLEKARAKAAADAAAAFAAEEAEGKE"
     gene            complement(35017..37440)
                     /gene="pbp1b"
                     /locus_tag="ACHU66_000269"
     CDS             complement(35017..37440)
                     /gene="pbp1b"
                     /locus_tag="ACHU66_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1B"
                     /protein_id="HFR3388585.1"
                     /translation="MATKSDKQDFKKKISALVLGDVVGVFLRTLKLLFNSVAVLVFLF
                     GLFGAGIGIGFVVSLFDDVKIPKTEELVAKVSEVSRISTVTYSDGSLVSEVNSDLLRV
                     PITSEEVSNYLKQAVIATEDETFETHNGVVPKAVLRAALGSVGLGSSSGGSTLTQQLI
                     KQQLVGDAPTFTRKANEIVSALALERNMTKEEILTIYLNVSPFGRNNQGRNIAGVEAA
                     AQGIFGKPAKDLTVPQAAFIAGLPQSPIVYSPYASDGTRKSDEDMVYGVERYQDVLFN
                     MYRASFLTKEEYETYKAYDIKQDFIAPAPVMADTKDYLYYKVMEEAQEVMFDYLVKRD
                     KVSKNDLKNDETKASYEELAKQELSQGGYTIKSTVDQGIYAAMQSVVANYGSVLDDGN
                     EYVETGSVLIDNATGAILGFVGGRDYSTNQNNHAFDTLRSPASTIKPLLAYGIAIDQG
                     LIGSASILSDYPTNFSSGQPIMYGSGRGTGMMNLQTAIDRSVNIPAFWTYKMMRNAGV
                     DAKAYMDKMNYHIPMYDIESVPLGGGVEISVLTNTNAYQTLANGGVYNKHYIVESITA
                     SDGTVVYQHEAAPVQVYSKATASIMNQLLRQVVNSGYTTTFKSRLSGLNPQAASSDFV
                     GKTGTSNEVNDVWLMLSTPRVTLGTWAGNDDNSEMYVWTGYHNNSQYVAHMVDALYNV
                     NADMFAGKFELDSSVIASSVVASTGQRAGTTQVNGRQVTVGGAMTTSYWAKNGAPVTS
                     YNFMVGGTDSDRAQAWNTIIGSQTPTSSSSTQRSSSTRSSTSSSANNTNQSSETQTAT
                     SEQANNDSP"
     gene            37594..38850
                     /gene="tyrS"
                     /locus_tag="ACHU66_000270"
     CDS             37594..38850
                     /gene="tyrS"
                     /locus_tag="ACHU66_000270"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR3388586.1"
                     /translation="MNIFEELKARGLVFQTTDEEALVKALTEGQVSYYTGYDPTADSL
                     HLGHLVVILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVTKIQ
                     GQLSRFLDFENGDNKAEMVNNYDWFSDISFIDFLRDVGKYYTVNYMMSKDSVKKRIET
                     GISYTEFAYQIMQGYDFYELNDKYNVTLQIGGSDQWGNMTAGTELLRRKADKSGHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDE
                     IAEIEKQFDAARHERLAQKILAREVVTLVHGEEAYNQALNITEQLFAGNIKNLSAKEL
                     KQGLSNVPNYAVQAEDNLNIVELLVTSGIVTSKRQAREDVQNGAIYVNGERVQDLDYT
                     LSDSDKIDGELTVIRRGKKKYSVLTY"
     gene            38908..39285
                     /locus_tag="ACHU66_000271"
     CDS             38908..39285
                     /locus_tag="ACHU66_000271"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFR3388587.1"
                     /translation="MTCIFCHQIEEIDILYQTEYFKVVWDIDPIQTGHLLIISKEHYD
                     TLSQIPSAVRYELSDLEVFLTGKLCQTLAIDGVTTARNDRLFDAGTHFHVHLIPRFRS
                     DGFWDQISLAQVQLDLNAFLKAL"
     gene            complement(39578..39787)
                     /locus_tag="ACHU66_000272"
     CDS             complement(39578..39787)
                     /locus_tag="ACHU66_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HFR3388588.1"
                     /translation="MKQTLKLKNLSCQNCVKHVTNHFLELDGVEEVKIQLEEQLAEVE
                     TTVAYDLEQYQEVLEDTIYEVEELV"
     gene            complement(39784..40230)
                     /locus_tag="ACHU66_000273"
     CDS             complement(39784..40230)
                     /locus_tag="ACHU66_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFR3388589.1"
                     /translation="MEQTISAAEWQVMRVLWAQPGATSQEIIQALQEGFDWQATTIKT
                     LLGRLRKKNYLRMAKETSKYHYYPLISEEEHLQGQVELLLATICSTKQGQLVEKLLDT
                     GIFSQKSLEKLASKISQLQRTAPEQIACRCLAGQCTCGHHAKGEKT"
     gene            complement(40330..41841)
                     /locus_tag="ACHU66_000274"
     CDS             complement(40330..41841)
                     /locus_tag="ACHU66_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFR3388590.1"
                     /translation="MKKVGLLFLSVSALLLGACGNSTASEDGKLNIVTTFYPVYEFTK
                     QVAGDEANVDLLVKAGTEVHGYEPSAKDIARIQEADAFVYENENMETWVHDVEKSLDT
                     TKVNVISATEGMLLLPGGEEEHKGHDHSGERHSHAYDPHVWLSPERAITLVENIRDSL
                     VAKYPEKKDAFETNAAAYIEKLDALDAKYSETLSAAKQKYFVTQHTAFAYLALDYGLK
                     QVSITGVAADEDPTPSRLAELTEYINKYGIKYIYFEENASKSVAETLAKETGVQLDVL
                     NPLESLTDEDMKNGKDYISVMEDNLTALEKTTSQEGSEILPEEGAETAQTVYNGYFED
                     SAVKDRTLSDYAGEWQSVYPYLLDGTLDQVWDYKAKIKGGMTAEEYKAYYDTGYKTDV
                     DQINITDNTMEFVVGDKKEKFTYKYVGYKILTYKKGNRGVRFLFEATDANAGNYKYVQ
                     FSDHNIAPVKTGHFHIYFGGESQEKLLEELENWPTYYPVGLTGLEIGQEMLAH"
     gene            complement(41851..42663)
                     /locus_tag="ACHU66_000275"
     CDS             complement(41851..42663)
                     /locus_tag="ACHU66_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFR3388591.1"
                     /translation="MFDLSVFHYDFMQRAFLAIIAMSLFSPILGVFLILRRQSLMSDT
                     LSHVSLAGVAFGLVLGISPTLSTVLVVIVAAVFLEYLRTIYKNFMEIGTAILMSTGLA
                     ISLIVMNKSGGKSGLSLEQYLFGSIVTISQEQVIALFTIAVIVIVLTLLFLRPMYILT
                     FDEDTAFVDGLPVRAMSIAFNVVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKSF
                     KAVIFTGMGIGFFGMVMGLLTSYYAETPASASITLIFISIFLLVNVVQKFKK"
     gene            complement(42656..43360)
                     /locus_tag="ACHU66_000276"
     CDS             complement(42656..43360)
                     /locus_tag="ACHU66_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFR3388592.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHIHYYLDSGEFVTLTGENGAAKTT
                     LIKATLGILKPKQGKVSIAEKSIKGKKLRMAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RQGWFRRLTAHDEEHVRISLESVGMWEHRDKRLGALSGGQKQRAVIARMFASDPDIFI
                     LDEPTTGMDAGTKDAFYQLMHHSAKKHGKSVLMITHDPDELNKYADRNIHLVRDQQSP
                     WRCFNVHEADEEVAHV"
     gene            complement(43361..43804)
                     /locus_tag="ACHU66_000277"
     CDS             complement(43361..43804)
                     /locus_tag="ACHU66_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HFR3388593.1"
                     /translation="MNRIVLEIEKYLHEIVLSSENQLEILVGSCQSTVKLTNTQEHIL
                     MLIEKAAYTNTEIAKELNVSQAAITKATKSLVAQGLLVAVRDDKDARIVRFSLTEAAK
                     PIATEHAHHHAHTLEAYEELLEHYSLEEQESIARFLSELVEKIRK"
     gene            44013..44544
                     /locus_tag="ACHU66_000278"
                     /pseudo
     CDS             44013..44544
                     /locus_tag="ACHU66_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377895.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT        12577 a        10530 c         8652 g        12785 t
ORIGIN      
        1 attctaaatg cacataatgt tatgaatcat tcggacattt tagtgtaaaa ataataccag
       61 atacttcaac gccaccaaaa aaaagaactc ccaactttac aaaaaaataa gtatcctata
      121 gttgattata atacgagaca agaagccaca agcataagtc gagcttaaaa aggtgagttt
      181 acgaagtaat actcaatgaa aatcaaaaaa atgattacac aacaaaattt ttcctccgag
      241 ccgacacttc agagcgtaaa ttgcatttaa aaagagaccg gagctcaagc cccagcctca
      301 tttcgtcgca attttttact tttcagtaag agctgcaagt cctggaagca cttttcacct
      361 tgctagactt tctaactcgc ttagcttcgt agaaactcta cgcgatgcac aaaatgcaac
      421 ggaaatccgt tgcattttag cctttaccta ggctgatttt ctaaactcac tacgttcaca
      481 gaaaatctag cctttgtact agaaaagagt tggtctccca actcttttca tcgttcatct
      541 ttcatacgca gtagctgaat ggcagttcac tttgctacgc ttgtgaccaa cctattcagc
      601 cttgcggggg tgcgtctacg gaatcataga ttcctgactt accgcctatt tttcagtcaa
      661 agctgcaagt cctggaagca ctttaccttc gaggagttcc attgatgcac caccaccagt
      721 tgagatccat gagaacttgt ctgcacggcc aaggttgatc gcagctgccg ctgagtcacc
      781 accaccgatg attgatttca cgcctggttg tttcacgata gcgtccatta caccgattgt
      841 accagcttgg aagtctgggt tttcaaatac acccataggt ccgttccaaa caactgtttt
      901 tgcaccagtc aacgcttcgt caaacttagc gattgactta ggaccgatgt caagaccaag
      961 gaagccttct gaaactgctt caccttcagt gtcgcgaact tcagtgtatc cagcaaatgc
     1021 atttgcttct tttgagtcaa ctggcaagat caatttacca ttcgctttct caagaagcgc
     1081 ctttgccaca tccaatttat cttcttctac aagtgagtta ccaatttcga taccttgagc
     1141 tttgtagaat gtgtaagtca taccaccacc gataagaact ttgtctgctt tttcaagaag
     1201 gttctcgata acaccaatct tatcagatac ttttgaacca ccaaggattg ccacaaatgg
     1261 acgctctgga gtttcaacag cttcttggat gtatgcaatt tcgttttcca aaaggaaacc
     1321 agctactgct ttttctacgt ttgctgagat accaacgttt gatgcgtgtg cacggtgtgc
     1381 agtaccaaat gcatcgttaa cgaagatacc atcaccaagt gaagcccagt atttaccaag
     1441 ttcttcgtcg tttttagatt ctttcttacc atcaacgtct tcgaaacgag tgttttcaac
     1501 aaggagaact tgtccatctt ccaaagcatt gatagccgct tccaattcag caccacgagt
     1561 agcaccagcg atgaaagcaa cttcttgacc caatttagct gccaagtcag ctgctacagg
     1621 agccaatgat ttaccttctt tgtcagcttc ttctttcaca cgaccaaggt gagagaaaag
     1681 aattgcacgt ccaccttgct caagaatata cttgatagtt ggaagagctg ctgtaatacg
     1741 gttatcgtta gtgataacgc catctttcaa aggcacgttg aagtccacac gaacaagaac
     1801 ttttttgcct ttcaattcta catctttaac agtcaatttt gccattagat aagaactcct
     1861 taaattatat ttactttacc attgtatcat attttctgta ttttttcttg cataaagata
     1921 tttttttcac aaatgttatg aaatctaaaa ctatcacctc gtaatcagat ttttcaaatt
     1981 ctaaaatcgc caaatcagaa acaactcatc tcattcatat ttttagcata agaaaacagc
     2041 catattccag caatccgaaa atacaaaaaa aagaggccga agcctcttcg attttcacta
     2101 aggattattt agcgattttt gcgaagtact caagagtacg aacaagttgt gcagtgtaag
     2161 acatttcgtt gtcgtaccat gaaacaactt taaccaattg ctcgccatca acttcgataa
     2221 ctttagtttg agttgcatca aacaatgaac cgaatgagat acctacgata tctgaagata
     2281 cgatttggtc ttcagtgtaa ccaaatgatt cagtagcagc agctttcata gcagcattta
     2341 cttcttcagc agttactttc ttgtcaagag ttgcaaccaa ttcagttaca gaacctgttg
     2401 gaactggaac acgttgtgca gcaccgtcaa gtttaccgtt caattctggg attaccaagc
     2461 cgatagcttt agcagcacca gttgagttag gaacgatgtt tgcagcagca gcacgtgcac
     2521 gacgaaggtc acctccacgg tgtggtccgt caagaaccat ttggtcacca gtgtaaccgt
     2581 ggattgtagt catcaaacct ttttgaacgc caaatgcatc gtgaagagct ttagccattg
     2641 gtgccaaaca gtttgtagta catgaagcac ctgagataac tgtttcagta ccatcaagga
     2701 tgtcgtggtt agtgttgaaa acaacagtct taacatcgtt accaccagga gcagtgataa
     2761 caactttttt agcaccgtta gcgtggatgt gttgctcagc tttttcttta gaagcaaaga
     2821 aacctgtagc ttccaaaacg atatctacgc catcagtagc ccagtcgatg tttcctggct
     2881 cacgctcagc agaaacttta acgaatttac cgttaacttc gaaaccaccg tctttaactt
     2941 caacagtacc gtcgaaacga ccttgagttg tgtcatattt caacaagtgt gcaagcatta
     3001 ctggatctgt aaggtcgttg atacgagtaa cttcaacacc ttctacgttt tggatacgac
     3061 ggaaagcaag acgaccgata cgtccgaaac cgttaatacc aactttaact accatgaatg
     3121 atttcctcct tatgaaaatc gagttcttta ttttaaaagg caagcctttt aatccttttg
     3181 tgaaaatatt aacttgtaag cctacaagca tcttttcaac gtttttatta tataactatt
     3241 taccgaaaat tgcaactgct taccggttca tcttatgtaa aaaatagacg gccaaaaaat
     3301 aaaagttaaa aatcatagcc atgcgttttc tcgggtggtt atgactggtt aaatacttaa
     3361 cttaatagca ttgccatacc tcgacacatt ttttaccgat tcttggttta ccgctacttc
     3421 tattttttac ctaaaaaggt gtctaccgcc cattttggac cgcagacacc tctataatct
     3481 tttaccacac tcgtaggcgg tcttctggtt tcacatacat tggatcccct tctttgacat
     3541 tgaagtctgt atagaattga tcaatttggt tgacaatgtt gttaacccga tatttagctg
     3601 gtggatgttc gtcgctgatg ttggcaatag caaattcttt ggtggtgact tcacgccatg
     3661 ctttaccata agactcgaaa actttcttcg tatcgtagcc ttttttctga gcaatatcaa
     3721 tggcaaccgt taaaccaccg agatccgcca tgatttcacc ggttgaaatt tcgccattga
     3781 catagtagcc cggagccact tccaatttag aatagatatc tgctgcctgt tgaacctttt
     3841 tattaaattt ttcaaggtct tctggtttcc accagttgac aaggttcccc ttttcatcaa
     3901 attgagaacc agccttatca aaggcatggg aaatttcatg accgataaca gccccgattc
     3961 cacctagatt ttcttcggga ctagcttttg gatcgtagag aggagcctgt aaaatccccg
     4021 ctggaaagac aatttcgttg ttagttgggc tgtaataggc attcaccgtg tgagaaggca
     4081 tgccgaactg cgacttatca aaaggcttgc caaattgccc catctcttca cgcaattcaa
     4141 gtgtttttac catatcgaag ttagaaagaa gacttccccc ttcttccttg gattggatag
     4201 caagttgtga gtaatccttc cactggtctg gatagccgat tttaacaacc aatgtatcta
     4261 gttttttcaa ggcccgcatc ttggtttctt cagacatcca ctgcactgct ttgattcgct
     4321 ctgcataagt cgctttaatc tcttgaacca tagataggat attagccttg gattcttctg
     4381 aaaaatggtg tttaacgtaa actttgccaa tcatatcgga gaaatttgca tcaatggccg
     4441 cttgagcaac ttcttcctga ctcggagtat ctaccgcacc agttgctacg ccgtcgtatt
     4501 tcgcttttgc ctcgattaaa ttgcttgaca gataatcatt gtaatgggca atcacacgat
     4561 attggagcag ggctttaaga gcgtctaaat tcttttcttg atagagttca tttaattttt
     4621 ttagagcatc tggttctgat acgactgtcg ttggagcatt ctgaagtcct aactcagaaa
     4681 ccaactcagc aatcggtagg ttgggaacca gtgctttgac ctcatcccaa gtcttctcgt
     4741 tatacatggc ttcaaagtta ttggcttctt cttttggccg ttgcactgca gccatctctt
     4801 tttccaaatt aaagaggagg tctgccttag aagaggcttc ttttgaggat tctcctgcca
     4861 agctaaacac ttcctgcaga taggctttaa aggctttttg gtttttcagt gaaaattcat
     4921 ctgtgccatc ataattttct tttggaaaac ttaacttgtg agggtcaacg tagatggtgt
     4981 ttttgttact gtcttttata tcttgagtaa cgtttatatg aagcaaattg cggatgtttt
     5041 tgccgtatag ggctaccaga atcttgttga gttcttccat ggtagaggct tttttaatct
     5101 cctctaaata aggcttgata ggctctaacc ccaatttgtt gcgagtatca aaatcggctg
     5161 ccaatcggta aaaatcaata agttttttct catcactacc agcttgcagc ttggaatagt
     5221 ccatatccag ctggtcaatc atctcctgga ttgcttcttg cgttttctca gagagttgac
     5281 tcagattatc tatttgcgat tcaccggcac ctaattttgt atctttcaac cattgctcat
     5341 tgatataaga atgaaaatcc gtctgtggtg ttgcttcacc tgctttggga gccgaggaat
     5401 tacttgtacc acaggctgtc aaaaatgtcg cacaggcgac tgctactaat gaaaatttta
     5461 atttttttct catgatttct gtcctttctt tttattgtta atgtgcttag taccactatt
     5521 tcatcaccat acaagcatcc tcctttagta attagcggtc ctaaactact ctttcatcga
     5581 tctaccttat aaggattcta acatttatat ctgcttttct tctgtagcct atctattaat
     5641 tctcttattt tttgtaacca ttcccaaaca aagtaattct aaatgattta aaacatttat
     5701 tttagcaaat tgagtgagag taggcaagaa ctcgaacggt caaaagagtt cagcaacaat
     5761 tcctaatttc aagttattaa gctaaaacaa tctattcaca gaccgtttta atccgtttat
     5821 ttcacagtag ccagacctag aagtcactgt tgagttcatc ggcaacagga atcttatacc
     5881 caatgaaatc ccaaaatagc aaaccctgat cttttgttag ccatccacac ctaaattagg
     5941 gtattttttt ataaattgta gctttctcat tgtatatttc caaaaaggat ctgacggata
     6001 cctatttttt agtcgtttag tttgttttta gcactcactt caaaggttca gggaaccttt
     6061 gaaggctaga gatagggcga agcaagttcg cctcaatagc tgcaggttgc tagaacagcc
     6121 tttaggctgt tctaagttag aaataagact tgcctcgcga atcacctctg taaagtacgg
     6181 caaagcgagt tcaaatactc cagtgaagtg ttcgaagttg aaaataggga aacgaagttt
     6241 catcgctcgt cgaagtaatc aaagataaac tactgatact caataaaaat caaaagtagc
     6301 ctaggaaacg aagccgaaga tagaactgga gttcatcaag gcaagttgac aacggataat
     6361 tttgatttcc gaagagtatg agtatcacgc tcagatgtgt gacatatccg tcgtaccaaa
     6421 aaacacctac ccgaaggtag gtgtagatag gcaattatgc attgccaccg tttttcttaa
     6481 tgatttcatc ttgtacagat tttggtacat cttcgtagtg gtcaaatacc atcatgaagg
     6541 taccgcgacc ttgagttgct gaacgaagaa cagttgcgta accgaacatt tcagcaagtg
     6601 gcacataagc acgaacgatt tgtgtgttac cacgagcttc cataccgtca acgcgaccac
     6661 gacgtgcagt aacgtgaccc ataacgtcac ctaggttatc ttctggtgca gtgatggtta
     6721 caagcatcat tggctcaagg atagttggtt gagctgattt agcagcttct ttaagggcaa
     6781 gagatgctgc cactttgaag gctgtttcag atgagtcgac atcgtggtat gaaccatcgt
     6841 aaagcttcgc tttaacgtca acgattgggt aaccagcaag aacaccgttc gccatagatt
     6901 ctacgagacc tttttcaacc gctgggatga attcacgtgg aaccacacca ccgacgatag
     6961 cgttctcgaa ctcgaaacct ttaccttctt cgtttggtgt gaattcgatc caaacgtcac
     7021 cgaattgacc tttaccacca gactggcgtt tgaagaaacc acgagcttgt gtagaagcac
     7081 ggaatgtttc acggtatgat acttgaggag cacctacgtt tgcttcaacc ttgaattcac
     7141 gacgcatacg gtcaacaagg acatccaagt gcaactcacc cataccagag ataactgttt
     7201 caccagtttc aacgtttgtt tcaacgcgga atgttggatc ttcttcagcc aatttagaaa
     7261 gggcaatacc catcttgtct tggtcagctt tagacttagg ctcaaccatc aattggataa
     7321 ctggttctgg aacgtggatt gactcaagga tgatttttgc tttttcatct gtcaatgagt
     7381 caccagttgt agtatctttc aaaccaaccg cagcggcgat gtcaccagcg taaacagttt
     7441 caatttcttg gcggctgttt gcgtgcattt gaaggatacg tccgatacgc tcacgtttac
     7501 ctttagaagt gttcaatacg tatgaaccgc tgttaaggac acctgagtaa acacggaaga
     7561 atgtcaaacg acctacgaat gggtcagtca taatcttgaa ggcaagagct gcaaatggct
     7621 cttcatctga tgcatgacgc tcttcttcag catctgtatc tgggttaaca cctttaatag
     7681 cagggatgtc aagtgggctt ggaaggtagt cgataaccgc atcaagcatc aactgaacac
     7741 ctttgttttt gaaggctgaa ccacacaata ctgggaagaa ttcaacgttg atagttgctt
     7801 tacggatagc agctttcaat tcttcgtttg tgatttcttc accttcaagg tatttcatca
     7861 tcaattcttc atcagtttca gcaactgctt caaccaattt ttcacggtat tcttcagctt
     7921 gttcaaggta ttcagctgga atatcttctt caagaatatc tgtaccaagg tcgttagtat
     7981 agatttcagc tttcatcttg atcaagtcga tgataccacg gaactcatct tctgagccga
     8041 ttggcaattg gattggatga gcatttgctt gaagacgctc atgaagtgtg cttactgaat
     8101 aaaggaagtc agcaccgatt ttatccattt tgttagcgaa tacgatacgt ggaacaccgt
     8161 attcagttgc ttgacgccat actgtttcag tttgtggctc aacacctgat tgtgagtcaa
     8221 gaacggttac cgcaccatca agaacgcgaa gtgaacgttg tacttcgatt gtaaagtcca
     8281 cgtgtcctgg tgtgtcgatg atgtttacac ggtggttgtt ccattgagct gttgtcgcag
     8341 cagatgtgat agtgatacca cgctcttgct cttgctccat ccagtccatt tgcgatgcac
     8401 cttcgtgagt ttcaccgatt ttgtggattt taccagtgta gtaaagaata cgctcagttg
     8461 tcgttgtttt acccgcgtca acgtgcgcca tgataccgat attacgagtt ttttctaatg
     8521 aaaattcgcg tgccatgagg tttgttctcc tatattttta gatttctatt ctgattatac
     8581 catattttaa gaaaagcgag tgggcaaatc ccacccgctt aagttcttac taatggtgtg
     8641 aagtggacga ggcaagcctc aatccgattt gtgatggttg aacgataaat tgctaccata
     8701 atctcaattg atttatgtag aactgactta acgtcctcac atcctaattg aactcgggct
     8761 acaactcact gacaaaaaga tgaacctcat cgattgcaca gcaatctgct tcgcttccct
     8821 attttgtcta ggagttgctt gacgccctcg tatcttaatc ttagttgaat tttagctaca
     8881 aatgtctgcc aaaaagataa aatctcctag aaactgaagt ttctgcgtca attttcctat
     8941 tttggctagc atttgtttaa cgctaaaata tcctatctta ccagcggaag tgtgcgaatg
     9001 cgcggtttgc ttctgccatt ttgtgagtat cttcacgttt cttaacagct gcacctgtgt
     9061 tgtttgctgc atccatgatt tctttcgcaa ggcgatcgat catagtgtgc tcaccacggt
     9121 tacgagcgat tgttaccaac caacgaagac caagtgttgt acgacgctct ggacgaactt
     9181 caactggaac ttggtagtta gaaccaccga cacgacgtgc acgtacttca agtacaggca
     9241 tgatgttttc cattgctgtt tcaaatactt caagtgcatc gttaccagtt gcttctttga
     9301 tttgatcaaa ggcaccgtaa acgattgatg cagcagtacc acgtttaccg tccaacataa
     9361 cacggttaat caaacgagtt accaattttg agttatacaa tggatctggc aatacttcgc
     9421 gcttaggcgc ttgattttta cgactcattt atatttcccc tttcttagcc ttttggacgt
     9481 ttagtaccgt atttagaacg gccttgctta cgatcgttaa cacctgctgt atcaagtgca
     9541 ccacgaacga tatggtaacg tacccctgga aggtctttta cacgtccacc acgaagaaga
     9601 acaacactgt gctcttgcaa gttgtgaccg atacctggga tgtaagcagt aacctcgata
     9661 aggttgctca aacgtacacg agcaaattta cgaagggctg agttaggttt tttaggtgtc
     9721 atagttccga cacgagttgc aacaccgcgt ttttgtggtg atgaaacgtt tgtttgaact
     9781 tttttacggc tgttgtaacc aacgttcaaa gctggtgatt tagatttttc tactttagac
     9841 ttacgtggtt tacgtaccaa ctggttaatt gtaggcatct acattctcct gtatatattt
     9901 tttatttttg gtgatagggc attggtgaca acccctatct gtgtgtactt ttgcaacatt
     9961 tgtcagcacg tccctgtaca ctcttgagag accaaaagta aaaagtaccg tctaatatag
    10021 taccacatcc caaagccatt gtcaaataga tatgtttgaa attttcaaga aaaaaacaca
    10081 gccaatcagc tgtgtcattt tgctattcct tagtaaccca tacccattcc gtccatacct
    10141 tgtggcatag ctggagcggc tggttctggc ttgttggcaa cgactgcttc tgtcgtcaaa
    10201 atcaagctgg ctacagaagc tgcgttttga agggccgaac gagtcacctt aacagggtca
    10261 ataatacctg catccatcat attcacccat tcacctgtcg cagcattaaa gcctgttcca
    10321 agctcagaat tcttcaattt atcaatgata actgatccct cataaccggc gttataggca
    10381 atctgacgta ctggttcttc caaggcacga aggacaatat tgcgtcctgt ttcatcgtcg
    10441 cctttcaact ccaatttcgc tacgctatcg ataacattga caagggctgt accaccaccg
    10501 gcaacgatgc cttcttcaac tgcggcacgt gttgcgttga gggcatcttc gatacggagt
    10561 ttcatttctt tcaactcagt ctcagtagcg gcaccgactt tgataacagc aacaccgcca
    10621 gacaatttag ccaaacgctc ttgtaatttt tcacggtcaa attctgaagt tgttccctca
    10681 atttgtgact tgataacagc cacacggttg gcaatggctt cagcattgcc aacaccttca
    10741 acaatagttg taccatcctt gtcaaccaca accttggcag cttgaccaag tgcttcaata
    10801 gtcgcatctt tcaagtccaa accaagatct tctgtaatca ctgtcccacc tgtcaagatc
    10861 gcgatgtctt ccaacatagc tttacgacgg tcgccaaagc ctggcgcctt aacagcaaca
    10921 acgttgaacg taccacgaat cttattgaga acaagggtag gaagtgcttc accatccaca
    10981 tcgtcagcaa taatcaaaag cggacgacta gtctgaagga tgctttccaa aagtggcaaa
    11041 atatcttgga tgtgagaaat tttcttatca gtaatcaaga taaatggatt ttcaagctcc
    11101 gcaaccatct tctcattatc tgttaccatg tattgtgaca gataaccacg gtcgaattgc
    11161 atgccttcta ccacatccaa ctcagtttcc ataccgcgag attcttcgat agtgattacg
    11221 ccgtctgttc ccacgcgctc catagcttct gaaatgtact caccaacttt ttcagaacgt
    11281 gaagataccg ctgcaacttg agcaatttca gctttattgg atacaggact tgcttgtgct
    11341 ttcaaggatt caaccgcagt cgcaactgct gcttcaatcc cgcgacggat accaattggg
    11401 ttggcacctg cagttacgtt tttcaatcct tcacgaacaa tagcctgagt caacacagtg
    11461 gccgttgttg taccgtcacc agcgatgtca ttggtttttg atgccacttc agataccaac
    11521 ttggcaccca tattttcaaa gtggtcttcc aattcgattt ctttggcaat ggttacacca
    11581 tcatttgtaa tgagtggtga gccataagct ttttccaaga caacattgcg accttttgga
    11641 cccaaggtta ctttgactgt atcagccaaa atatcaacac cacggaccat gctttcacgt
    11701 gcatctgctg caaatttgat ttcttttgcc attttcctac ctcttattct acaattgcca
    11761 aaatgtctgc ttcacggatg atgtgaacgg tctgttctcc gtctttgact tccaatcctg
    11821 cgtgagcatc aatcaaaaca gtatctccaa ctgctaccgc tggggcaacc aagtcaccat
    11881 tcaaggtacg aatcccttga ccgatcgcta ggacacttgc ttcttttgtc ttttcttgac
    11941 ttgcacctgc aaggacaaag ccaccaactg tttgttcttt ttcttcaatt tttacgacga
    12001 tacgatcgcc caatggtttc aacatcttat tacctcctgg tctttttagc actctacact
    12061 cgcgagtgct aactcacatt tcctattgta tcacttggtc aaaaatagtc aagaaaattc
    12121 ttttttaatt tacctaaaat ccgtctaggg actgaggaaa cgcaacctgt taattatggc
    12181 aaaactatgc tatactatta ttaaaaagtt aaggggggat ttatgaaatt ttttagagag
    12241 ctaaaaacag attacctaga aagccgtttt tccgttcacg aaagttttgc agaatggttt
    12301 ttaaaacgta aattaggctt tttggggaaa ataatgttcg cctatctact ctggctagtc
    12361 tggttgcttc tcttttccca ccctcattac atcatctttt tcttctacgg agttctccta
    12421 ctttctctaa ttatcatgct gatagaatgg tggaaatata gaaaataagt agcaaaaaac
    12481 gccaactttc gtcagcgttt ttaaggagat atgaaaaata tttaggattg actatagtat
    12541 agcctatttt tcaatacgct ccatcggtct tgggactgat attaaaacgg taattcctcc
    12601 tcttctaaga ccaaatcagc tagatcatcg ccagcattat tttcacgcat agcacgttgg
    12661 gcccgacttt cgagcagctg gaaggattgt cctaagattt ctgtcacata gtggttgctg
    12721 ccgtcttttt cgtattttcg cgttcgcaat tcaccatcaa tggaaattag actaccttta
    12781 ttaccatagg aaacaagcgt ttccgccagt ttgccccaga aaatgacatt aagaaaatct
    12841 gcttcacgtt cgccattttc cgtcttaaaa cggcggttga ctgcgacagt aacacgggtc
    12901 agatttttgc cgttaggggt ttgagtgagt tcaggttggg ccgtcaagcg accgattaaa
    12961 ataactttat tatacatttt atatcctcct acttatttat tcgtagaagg attagaaaag
    13021 gagcgaaaat tatgaaagaa tttttgatgg aaaagataca cacctcccaa ccatatacta
    13081 atgccgaact agcttggttg ctggaaaaca tcagtcatcc tgatcctgct atacgcgacg
    13141 aactggttta tgctagtttt tgccacatct ttttggaggg cttaatcaca agggagcaag
    13201 cacagtcgct actacaattc tctcaagaaa ccaatcacct ttccctagaa agttccacac
    13261 taaaacgaag ttttacttgc ttgctctact gtcttttgct atctgttgat aatgagcgag
    13321 attctgtgta ctttgcattt ttaagcacta acgaccgtga gctactcttc cagcaggcct
    13381 tggattattt ggcgattgaa aatgactggt ctggctacga tgaaaaactc ggctggatcc
    13441 acaccgctgc ccacggagca gattttcttc tggccacaag ctgtcatgat caatttcccg
    13501 ctgaaaaaag caaagaagtc tggcaaacta ttgtcacttg tctaactaga caaagtaaag
    13561 ttttctcagc tggcgaggaa atccgtctgg cacaaattcc tgtatattta ctactgaatg
    13621 aaaaagtaac cagcaaagag ttgacagaat ggattagcaa gctggacttt ccaaatcaag
    13681 aaccagtaga ctacttccgg tggctcaatc tccaacattt tctgtctagt ctttacttcc
    13741 aacttaggtc acatcaagca tcgaccgaag aaatcgaaca ggctattgaa ggaaaaattg
    13801 aaccagcata aaagaattgg ccaaagcgac caattcttct ttttctatcc cttccagtgt
    13861 ttagctggat caagtgcttc tccgactact gcggagtcgt ccaattcgat aatgccacgg
    13921 acttgtggtg cgtttggcag agccaattca cgcggactac acatcatacc aaagctatct
    13981 tcaccacgca atttccctgg gaaaatcagg ctaccgctcg gcatcattgc ccccggaaga
    14041 gccacaatag ttttcaatcc ttgggcagca tttggtgctc cagctacgat ttgaacggtt
    14101 ttgtcaccaa cattgacctg acagatattg aggtggtagc tgtctggatg cggaaccagt
    14161 tccacaatct gaccgacaac gaatttcgga ctattgtcgt tgactaagct ttcagtaaaa
    14221 ccttccttag ccaattcagc attcaaggtc gcaacttgct cgtctgtcat gaaaacctga
    14281 ccatttccag caagctcaac caaacttgat gcttcaaaga tattccaggc tactgttttt
    14341 cctgtttcct ctaggaaaac acgcgccact tgacccttgc gttcaaactg aacaggctga
    14401 cccttgtctt cagccacaat catcataaga acatcgccga cgtgttcctt attatatgta
    14461 aaaatcattt ctttttcatt tctcctttat gcaaatagac cggtcaagcc tttccaaaga
    14521 ttttcaaaga aggcaccgat tttttctaaa atccctgaat cttgtagatt cttataggct
    14581 tctgaaattt gttgctccgc ctgttgcttg aactggttga gttgggcagc aacctcagcc
    14641 gaatcaatag cagaggtttc ctgataagct ttggcaaatg tgactagctt agaaatttca
    14701 tctgctgtga tgacattttc taaaccgttt tgggtcaaaa catcattgat gatggtgttg
    14761 atgtcagcct cagaagccac ctgaccattg gactttttgt agtcggcaag agcagtcttg
    14821 atttccgcca ctgccttatc caaagcagag ctgtcaaatt gttcatcacc agcatgggct
    14881 gtcgctactt cattaacagt tgttaattct tgttgagcta cttcagttcg agcggtatca
    14941 actgtgtcac cattggctgc aagagccttg taaacacctg tcagagcgga ctctcccgtc
    15001 acctgaatgg gtgccgcaac gtcaatcaag acatctgtcg caccggcagt aatggccgca
    15061 ttggcatact gcgtttcagt aatcaaagtg atgttctgcg gtgtcttaat gtccaccaca
    15121 acccctttgc cagcgtcctg cttttgcacc aagacagatg aatagaggga ggctgtgtcc
    15181 gctccagatg tacctaaata ttggtccata tccgtgccaa caaccacctg acggctaaca
    15241 ttattaacat ccgaaatacc aaatagattg ttgacctctg tcgcctgcgc atctgacaaa
    15301 cctccaccgt agactagtgt tggcttgccc catttttcat tgataacatc tgtttgaatc
    15361 tcagcctgca ccaagggagc aaggctcaaa ctcaacccca gcaccacagg tgctagaagg
    15421 atttttccta gtgatttctt catgttcttt cctttctttt gggaaatggc agagcttctc
    15481 tgctaattta tctacccagt ccagataaga agttcatgat ttcttccttg gtcttgcgat
    15541 tcttatcgac aaaacgaccg atttcctgac cattttccaa aacaactaga cttggaatgc
    15601 ctaaaatagc ccattcctgc gccaaaggca taaagtcatc tcggtctagt tgcacaaaac
    15661 ggaaatctgg aaaagcctct tcgatagctg acaaatgcgg tttgatatag tggcagtctc
    15721 cacaccaagt cgtcacaaat agaaaaacag tcggcttacc atcttcaatc aagccagcta
    15781 cttcttcaag atttgttgga aaaatcatct ttcttcctcc ctgtattgaa tagagccttg
    15841 ggcattttca aatagaaaca cctgtcctgc ctccatcaca actcctccaa cgagagagtc
    15901 ttgcgttgag gctgtttcat ccacatagac cgtcgcaatt tctcctacat tagcgaaaaa
    15961 gtcacgcacc tcctgcaaag cctgtgcttt tttacggtct tcctgaacct tacggtaaaa
    16021 ttgagcacta gcggtgagca cacctgcccc caataagcct gctagcaagg ctgttctttt
    16081 ctttttcatg ttgactattt tatcatattt cctctgaatt ttcccaaaaa tgccattgga
    16141 cttgtgacag ctgagacaaa ctctccacaa acatatgaaa aagattcagt tttcagaaag
    16201 catgatttca tcatgtgttt attgtatatt tttccatatt ttcaagacaa gtctatctct
    16261 tcgtgataaa atagtagtaa tcatgtcatg gaggaagaag aatgactctt tttgaaaaaa
    16321 ttaaagaagt tacagaatta caaagcttac caggctttga aggacaggtt cgcaaccata
    16381 tccgccagaa aatcacaccg catgtagacc gcatcgaaac agatggacta ggtggaattt
    16441 ttggcatcaa ggatacaact gttgaaaatg ctcctcgcat cttagtggct gcacatatgg
    16501 atgaagtcgg ctttatggtt agccagatca aaccagacgg tacttttcgt gttgtcgagc
    16561 taggtggttg gaaccctttg gtcgtatcga gtcaagcctt tacgcttcaa ctacaggacg
    16621 gtcggaccat tcctgccatt tcaggttcca tccctccaca tctaacacgc gggggcaacg
    16681 ctccaggcgt acctgccata gctgatatta ttttcgatgc tggttttacc aattatgacg
    16741 aagcttgggc gttcggcgtt cgtccggggg atgtcctcat tcccaaaaat gaaaccattc
    16801 taacagccaa cggaaaaaat gtcatttcta aggcttggga caaccgcttc ggtgtcctta
    16861 tggtgaccga actactagaa agcctatccg gtcaagcgtt gccaaatcag ttgattgctg
    16921 gggcaaatgt gcaagaagag gttggtcttc gtggtgctca tgcctctaca accaagttca
    16981 atccagacat tttcctcgct gttgattgct cgcccgcagg tgatatttat ggcgaccaag
    17041 gaaaaattgg tgacggaact ctgcttcgtt tctatgatcc aggtcatatc atgttaaaaa
    17101 acatgaaaga cttcctcctc acaactgccg aagaagcagg tgtcaaattc caatactact
    17161 gtggtaaagg tggaacagat gccggtgcag cccacttgaa aaaccatggt gttccatcaa
    17221 caactattgg tgtctgcgct cgctacatcc attcacatca aactctctat agcatggatg
    17281 acttcttaga ggctcaagcc ttcttgcaag ctatcgttaa aaagctagat cgctcaacgg
    17341 ttgatttgat taaaaattat taaaaacaaa agactttgct aggtagaacg cccagcaaag
    17401 ccttttttct atataaactc ttctaatata tacttcctca cataagtaat aaagtaaagc
    17461 gagcctgtaa tcaggtaaag ttttttggga ttgtccaagt ccgcctgctc caaccactct
    17521 tgataggtct gaactcgttc ataacctgac ggataatcat ccagctgtac cgcacggaaa
    17581 tcatcaaagg ttgtcacact aacaggtcca aattgactaa gctgcgataa catctgatcc
    17641 acaggcttgg tattgatggc tgcaaagagg atttcgatgt ctctgtccgc atatttttcc
    17701 tctaggagct gggtcaggac ggcgatactt tcattattgt gggctccgtc aatcatgagc
    17761 catggttgca tcaattctaa gcggcccggc cagatggctt gggacaagcc ctggctgatg
    17821 gtttcattga taatctttgg aaaatgtgag ctaagaagct gagctgtggt cactgccaag
    17881 gctgcattga cctcttggtg acgcccctgc atcaagactt tcaactcttc cacacgaccg
    17941 agaggacttg aaactgcaaa gcctgctctg ctatcttcca aaagaatctc ccgccccacc
    18001 gcataggtcg gactctgaag ctggctggcg tgatcctcga aaactgcctc cacttctggc
    18061 aaatcgatcg catagatgag aggaaccctc tcacgcaaga cggcaacctt gtgacgggct
    18121 atggctgcgt gggtctctcc caaaaaaatc tgatgatcca agccgatgga gggacaaact
    18181 actgccaagg gggcaaggac gttggtcgca tcctgcaaac cgcccatgcc tgcctccacc
    18241 aagagaatat cgggacgctg gtagtgggca aagtagacaa acatagccac tactacaatc
    18301 tcaaactccg agatggcatc cagtccagcc gtctggtcca agtcctcaat caagggttgc
    18361 aagtctgtca caatcctcgc aaaatctttc tccgaaatca tctcttgctc aaagacaatc
    18421 tgctctcgaa aatcaatgat agacggtgag gtaaagcgac cgacggtgta acccgaagac
    18481 tgcaagatag actgtaaggc gttgagggtc gagcccttgc catttgtacc aacaaagtga
    18541 atagtcggca cttgaagctg gggattgccc aagcgttcca aaatccagtt gacacgtgca
    18601 acgccatttt ctaaatctga tgcaggacga ctagatagcc accggcgagt ttcttgataa
    18661 ttcatggttc cctctctaca aaagcagcaa cagggagatg acctgttgct tgatactgtt
    18721 ttgtttaatg cccactaact tggccaattc aggtattttt tgtaaacttg gcaagacaag
    18781 catggtaacg acaatgcgta atggaatttc aaaaatagct tttatgaaac gactgctata
    18841 cagggcaatc caaggtgttc caaaatggaa atggagggca atcggtgtca tgataaagga
    18901 aatcacaacc cgaatcaaga caacaacccc tgcaacatag agccaatcag ccttgttctt
    18961 ggtgtctagc ggttttcgat agaagaacca gccatagata aaagccgata ccgcctcaat
    19021 caagataaaa ctaaaaagca tgctctgtcc actaaacaag acaaagactg ggtctgaaat
    19081 cgccgcaaac acagctgccc aaacaggacc agcaatcgca cctaaaatag cattagggat
    19141 aaaggtaaac tggacctgca aggtatcaga aagacgaaca gaaaaatagt tctccacaat
    19201 catgaccaag gcaagggtca cagcaattgc agccaacaac tgcaccgtta gttttggaat
    19261 tttcttttcc ataaaaaatg ctcctttttc aaaaaatgga gctgtcgtgt gtattcgaca
    19321 gcggatgcaa tggtttaccg cgctactctc agccaggacc aatgttccta ctataggaga
    19381 catcagacac aagcaaagat cagcttcgtt atgtggcaac atcccatcca catacacttg
    19441 acgcaaacca tctactctgt gcaaatttct ttgctagtct attgtatcac acactatata
    19501 ttttgaccac ttgctaaaaa ctataccttt ctaaaggtat aaactataag agaaactggc
    19561 agtctccgac taccagttag tttttcttat ttcaagcgtt ccacttcacg cgcaataacc
    19621 aattcttcat ccgttggaat aaccaagaca cgaacttttg attctggcgt tgaaatgtca
    19681 ccatagttgc cgaatacatt tttttccggg tccaactcaa taccaaacca agacaagcct
    19741 tctactacgt cattgcgcat aagcggtgca ttttcaccca taccagccgt gaagacaata
    19801 gcatctgccc catttaaaac tgcaagatac tgaccgataa atttcttaat acggtcaatg
    19861 aaaatattgt aggccaacac tgcatctgga ttatgagctt ggatacctgc ttcaatatca
    19921 cgcatatcac ttgagaagcc agatacaccg aataagcctg actgtttatt caacatatta
    19981 acaacgtctg cagcatcttt cagttcttca acattcgcaa tcaaataagg aatgatagca
    20041 ggatcaatat cccctgaacg agttcccatc attggaccag ctagtggtgt gaagcccatt
    20101 gaagtatcca cagattcacc atgatagtta gcagtgatgg atacaccatt tccgatatgt
    20161 gccgtaatca attttaactc ttcgattgga cgccccaata cttttgctgc ctcatgcgct
    20221 acataatagt gactagttcc gtgagcccca tacttacgaa ccttgtggtc ggtatagtac
    20281 ttttgtggaa tcggataaag ataggcatgc tttggcatcg tagtgtgaaa ggctgtatca
    20341 aaaacagcta cattcaaaac ttctggtagt acttcctcaa atgcacgcat tgctgctacg
    20401 tgagcagggt tatgaagcgg tgccaaagca cttagttctt caatttgctc aacctctttt
    20461 ggtccaataa cagctgattc tttgaaaatc tctccacctg caacaacacg gtgtccgata
    20521 ccagtaattt cctcgtaagc agcgatgatg ttgtgtttaa gcaagtcttc taggagaatc
    20581 ttaaccgcaa ccgtatgatt aggaatatct aaaacttcac tttctttctt cccatcatac
    20641 ttaactgttg agatggaatc attcaaaccg atgcgctcaa caatccctgc cgctaatact
    20701 gtttcttctg gcatttgata aagttgccat tttagacttg aactacctgc attaattgca
    20761 attgtttttg acatatctta cctcttttaa agcgttttcc taactagtat aacagatttt
    20821 cacttaaatt gcattctctt gcttccaatt tttgaaattg agcatgaact tctgaatagc
    20881 ttctggttct tgaagacttt gtaagggata aacaaacggc gtcaacgatc ttgcagcttt
    20941 cttttgcaag acaaaaatag acttggccat agcagccttc ccaaagagat ttggtggcag
    21001 ggcaatcatg gcaacaatat tggcttgctc ctgtaaccaa cctttcagca aatcgctttg
    21061 cggactagtc aataaatcat ttggagccaa caaaatcgca aagccatctt tttccagata
    21121 tttcagggat tgttccatga gtaaatgatg ggcgtaggta tgttcatttg ggctggcgac
    21181 ctgatagcgg ctagcaatct ggtcatctgg atagtagcca ataggcaaat ctcccaaaat
    21241 tacttgactt tccttcaaaa tctgcggacg taccgcatct ccctgagcaa aagaaatatc
    21301 tgcctgcatg acatccgcaa tacttgccga caaatcaatc aagaggtcgt ccacttcgat
    21361 ccccaagtaa tccaattctt tctggctggc gttgagaatg gtctgagcta gattgcctgt
    21421 ccccgaacca atttccagaa ccgtcacctt ttgagtcggc atcaattggt ctactagaaa
    21481 agataggata aatccgatag agtctggtgt gaactggtga ttgtactgca tgggttcagt
    21541 ctgattggcc ttgatgagca agaattgata ggcacgacgc cattcttcct tggttaaatc
    21601 tagttgtttc aaggtcttgt tattattgac aacaaggtcc atctcatgct gattagctac
    21661 gtaagcagca ttttgctcaa tcatggcatc atagatattg gtacctagct ggttttggat
    21721 agtctgtacg ttttctaata gcaggtcgta agcctgttcg atcttttcaa aattcatact
    21781 cctatcttat caaaatttca cagactttgc accaaaaagg agagcgagtc aactcaccct
    21841 ccttctgagg atagccccaa aggaaattga taggtataca gaccatcact tgttaactca
    21901 actgtaacca atagttggtc tcctttaagt tgataacgag cctgtccctc ttgaaaggag
    21961 acccttcctc tattctcctt ctgcttagaa cctgtggtta aatttgcttc cttttcattc
    22021 actaccgctc ccgtaactat tttttctact tgcaggtcct gctcgacctt atccaagacc
    22081 atctgagcca tgatgtaggc agtcgtttct tgccttgcca cctgtaccaa gcgactctca
    22141 gcctcttggc ggtggagata aaactgaagc aagagactaa aaaccgccag catcaaaagg
    22201 gcatagagta ggatgccagc cttaactttt ttctttaaaa gcatagataa aggtccgctc
    22261 ctctccgttt ttaaagctaa agtcgatgcg caccagctgg ttttcttggg aaatggctgc
    22321 gctatccacc tgatagagca tgggttggta accctggcct ctatcatttg ttttccgaaa
    22381 atcatccgac catgacttgc caaaggccaa agcctgttca cctttgttga catagacctt
    22441 gccattttca accttgacca attcagcctg gtcccactcc gaccgcaatt ggctagagaa
    22501 gaccaaccat tccttttgaa cggtctgacc ttgataatgc gcttcttgga ccagcagttt
    22561 tgacaagcct tcaaagacca gaagactacc tgaaaggatg actaaggcta ccaagcattc
    22621 caagagagta aaagcaggaa ttttactttt taacaaccga taaaacctcc tttccctcgt
    22681 agaaaacaat gatcttatcc gctgtttgtt gaacctgaac tgtaacaccg tttaaggcta
    22741 gcttatcttg cccggtctga acagccatct gtgctagatt gagaacctcc tgttgttcca
    22801 attcccaagc ctgtcgccta cgacctacat ccacagctga taaaatcaag ctgcaaatgg
    22861 tcactagagt tgcaagggca accagacttt ctagcaaaat ataagcctta ttcttctgtt
    22921 ttcttatagc gaccactccc tatatataat tgatacgtga ctattttttg actaaggcga
    22981 aaacgaatct tggtcagaga agaatttcca ccatcttctt caaatcggat agctttctcc
    23041 tccaagattt ccacgtttct tggcactttc actatctgac tgccatttga cacttcttgt
    23101 ccaccgatag aaagactaac tgcttggcgg gaactggctg ccaatttctg actgtcttta
    23161 tagatggcct caaattccca gagaaaaatt tcttcctgaa cagaccgaaa gaccgtttgt
    23221 actgttcccg acaggctgat tgccagaaag ctgacaacca acaaggtcag cagactttca
    23281 aacagggtaa aagccttatt ttttcgccgc agcatactgt tttgcttgtt tttcagtgat
    23341 gtagccagct gcctgcaagt ctgccaccgt tgcttccttg tcatgttcca attcatagag
    23401 ctccatctgg ctctccacta ctttgacaac agccgcatcc cccttcttct gaatagcatc
    23461 cttctgctgg ctcaaatttg gcacaaaaag gagcaagagc aggctaatga tccccaaaac
    23521 gactaacatt tccaccagag tgaacccttt taccttcatt tcaactaatt ttttcatttt
    23581 acaactccat attttgataa atcggcagca acatggctgc gtagatgaga acgaccatta
    23641 aggccacaaa gagaaagacc agcggttgaa tcaactgcat ggctctattg acccgagaga
    23701 aaaaatcctc ccaacactcg gctgcataaa ctgtcaactc gccacccaac ttggacttga
    23761 cctgaccata ctcgacaatc aaactcagct cccgcttaaa aaaggcgtag gtcttaatgt
    23821 gttcgtgaaa gctctgccca ttggaaagcg actgctccag gtcctgccca atctcccgaa
    23881 agagctgcga ttgctgctcc tgcatcaagc ccacaatctg tggcaggtcc aagccttgcc
    23941 caatcaaact tccccactcc ctggcgtaat aggccgtcag atagatttgg atgagttttc
    24001 cgaaaaatgg cagagctgcc aagcgggaaa atgccttgat tttgttggtt ttgcgaaacc
    24061 aaaccatacc agccaagacc gccaccaaac taagtccaca gagggataaa aagatagtcg
    24121 gcagatggtt aattagcatg gtcgcagcat tgccttcctc caactggggc agaagatagt
    24181 ttttcaagcc tagcataatc aagaacagaa aacccagtaa gataatcgga taggtcgcca
    24241 cctcaatcag ctttttacga accttgctga cattttccag ataggactgg atatggctca
    24301 aactcaggct ggtattacca tgaacttctg ccagagccac ctgtgtgacc accgcatctg
    24361 aaaaccgcaa atctgccagt aaactcgaaa agggtttgcc tgccagcaac ccgtctgaca
    24421 agacctgggt atagagatct gccagaagct gactgcgttt gaggaaatca acaatctccc
    24481 ccagatgaaa accactagca aaaagattat tgaaaagctc aatgacctta cgctggcgag
    24541 ccaagggcaa ttttttctgt ttctgcctgc ctaagactga tatgtcctgc tgcaaaaagg
    24601 cgatcaattt gcgcattcca ctggtctgcg gaatggtttt ggtaatttcc ctttgcaaaa
    24661 tctaccacac ctcctcccct gattaaacgt tgataggcaa taccttgaag ggcattgttt
    24721 aactcctcag gactgacccc caattccaac atacgagcat agacacccga aatagacctt
    24781 gcgtgaaccg ttgagaagac cgtcgctcct gtcagactgg ctcgaatgac tgctcgagcg
    24841 gtttccgcat cccgaatttc accgatgatg agcaagtctg gtcgatgacg gagggacagt
    24901 ttgatcagat tgtcgtaggt ggctccgatg gcttcattga gctggagttg cagcatgtcc
    24961 tcctgtttga tttcgacagg atcctcgatg gtcaaaatct gcttgtctgg gaatttcagc
    25021 ctggcaagat ggtacataag tgtggtctta ccagagccga ctggacccga aaaaaggtag
    25081 agccctcgtc ccttgatttc ttctgcaagc cgctcagccg cctcaaacca gaacttgagt
    25141 tccttgtcat tgtcatagag caagcggatc accagacttt ccttgccacg ataatctccg
    25201 acagttgata agcgaagtga aatctctccg ctaccataat catagtcaca ggaaccctgc
    25261 tggctacggc gtttttcacc aacatttaaa cctgctaaaa acttgaaatg gctgatgatg
    25321 gctgttactt cctcctcagc caagtcttgc acaaactccc gttcatccat gatgcgatga
    25381 taaacttgat agctttcccc gcgaggaacc agataaatgt cactgaccct atccgccact
    25441 gccttttcaa tcaactttct tgctttttct tgaatcataa aacctcctca ccctatctat
    25501 tcgcaaggaa acttgatttt ccatactttt ttgttaaaat aataaaaaaa cttggaggtt
    25561 cctatgaaaa cattaaaaaa aggcgaccac atacgtgtag tcagtccctc ttcatctatt
    25621 gaaagaattg gtggttttga agccaatctt gctgccaagg aaaaattgga agacctgggc
    25681 ttcaaactct ctttctcaga acattatttt gaaaacgata ttttcgattc cgctccaatt
    25741 gccagtcgtg ttgcggactt ggaagtagcc tttgcggatg agtcagtcga tgccattctg
    25801 acaaccattg gcggtttcaa ctgcaacgaa ctcttgccct atctggattt tgacctaatt
    25861 gcgcgtcatc caaagatttt ctgtggttat tcggacacaa ctgccctgct caacgccatc
    25921 tacgccaaga caggcatgcc aacctacatg ggtccagctt attcaagttt caaaatggat
    25981 ttggggcaac agtatcaaac agaagcctgg ctcaaggctg tgacacaaga ctcctttgac
    26041 ttgaccccga gtccagaatg gtctagcgac gcttggtacc taccagacgc ccctcgcacc
    26101 ttttacccaa cagaatggaa agtctacaat ccaggccagg cctctggtat tgccattggc
    26161 ggaaacatct caaccctcaa cctgctgact ggaacagagt ttgctcccag accagacgaa
    26221 tatattctat ttttagagga ggcggaagac gacgattatc tcattatcgc tcgtcacttg
    26281 actacacttt tacaagccta tccaaatcca caggctcttg tcttcggtcg ttttcctaaa
    26341 gagaccaaga tgacggagga gattttgctg gcgattttgg acaagcaccc tattctcaaa
    26401 accatcccag ttctctacga tttggatttt gcccataccc agcctctgtt taccattaca
    26461 atcggtggtc aagtggagct cgacaccaaa gccttttcta tcagattcag ctaaaaagga
    26521 ttgacccgca aaggtcaatc ctcttttatt attcatttac gcaattattt tgttttaatt
    26581 tgcaggggcg tacacgaggt tgcgtaggtt gcttaataaa accagccggt aaattttctc
    26641 tcgcttctac caacatcagc gaatgatacc gctgcaaata ttcatcgcat tcctcgcacc
    26701 aatgctggtc tgattcatcc caaaaagcca ccatcgttcc attcttactt ttcttcattg
    26761 gaaaatcctc tgatagccgt acccactctt tttgaaaggc attcagtgca tcctcatagc
    26821 gatcaaaagc agccctactg gtaacatcct cttcccaacc atctaagaac caccagggct
    26881 caaaatctcc atacatttta attacttgat acataacaca tacttccttt ttcgtacaca
    26941 ttataccgaa ttcgtctgat ttaagaaaac attctgcttg ttaattcctt atcaagttgt
    27001 gtgagcgctt tatttctgaa cgaaagaaaa cacatccctc agaaagagat gtgtctcaaa
    27061 tcttattcag ttacagcttc aagctctgct tctgctgcaa gttcttcagc aactgtgtct
    27121 tcaatgattt caacttcatt gatggcttgt ggttcaagat tacggtagcg ggccatacct
    27181 gtacctgctg ggataatctt accgataata acgttctctt taagaccgag aaggttgtca
    27241 cgtttaccac ggatagcagc atctgtaagg acacgtgttg tttcctggaa ggatgcggca
    27301 gacaagaatg agttggtttc aagagacgct tttgtgatac ccataagaac tggacgagcc
    27361 gttgctggaa caccaccagc gatgacagtt tccgcattgg catcagtaaa gtctgagata
    27421 tccatgaggg tacccatgag caggtctgta tctcctggat ccatgacacg aactttacga
    27481 agcatttgac gaaccattac ctcgatgtgc ttgtcgccga tttctacacc ctggctacgg
    27541 taaacttttt gaacttcaga aagaaggtaa gtttcaaccg ccaagacatc acggacttca
    27601 agcaagcgtt ttggttggat agaaccttcg gtaagggcgg ctccacgcgc gacttggtca
    27661 ccaacttcaa ccttcatacg ggctgtaaat ggaaccacat attcgccttc gccagtttta
    27721 cccttaacaa agactttctt ggtacgagta gaagcatctt cttcaatagc gataacttca
    27781 cctttaacct cagtgataac cgcttcccct ttcggattac gggcttcaaa gatctcttgg
    27841 acacgaggaa gaccctgcgt aatatcgctg tttgaggcta caccacccgt gtggaaggta
    27901 cgcattgtaa gctgtgtacc aggctcaccg atcgattggg cagcgattgt accaactgct
    27961 tcacccactt caaccgcatc acctgttgcc aagttgatac cataacaatg acggcagaca
    28021 ccgtgacgag tgttacatgt aaatacgcta cggatagtta cttgttcaac acctgcattg
    28081 acgatttcac gggcaatatc ttccgtaatc aattggtttg gaccaatgat aacggcaccc
    28141 gtttcaggat gtttaacagt tttcttagtg taacgacctt gcaaacgctc ttcaagtggc
    28201 tcgatcattt ccttgccatc tgtgattgaa cggatatcaa gaccacggtc tgttccacag
    28261 tcgtcttcac ggataataac gtcttgggca acgtcaacca aacgacgagt caagtaacct
    28321 gagtcggctg tcttcaaagc cgtatccgtc atccccttac gggcaccgtg agttgagaag
    28381 aacatttcca aaacagaaag accttcacgg aagttagaca agataggcaa ttccatgata
    28441 cgtccgttcg gagctgacat cagaccacgc ataccggcca actgggagaa gttggaaatg
    28501 ttaccacggg caccagagtc catcatcata acgattggat tcttcggatc ttgtttttca
    28561 accaggcgtt tttccaattt ttccttagct gaacgccatt catctgtaac agctgcataa
    28621 cgctcatcgt ctgtaatcat accacgacgg aattgtttct tgatttgttc tacacgttcg
    28681 tgagattctt caatgatttc agccttgttg tcgataactg ggatatcggc aatacccact
    28741 gtcaaaccag cgagtgttga gtgataataa cccaagtctt tcaaacggtc aagaagggct
    28801 gatgtctcag ttgtacggaa acgcttgaag atttcagcga tgatgttacc aagatttttc
    28861 ttcttgaatg gtgggttgat tggcaattct gcaatggcag ccttgatatc tgaacctgat
    28921 tccaagaagt atttatcagg agttccttct gtcaagttag cattgtttgg ctcttgcaag
    28981 tatggaagac cttctggcat aatcgcgttg aacaagattt ttccgacagt tgtcatcata
    29041 atcttgtgtt tttggttgtc tttccaaggt ttatcaaggc tatccgttgc gataccaaca
    29101 cgagtatgca agtgaacata gccattgcgg taagccataa cagcctcatc cgcatccttg
    29161 aagaccatac cttcaccttc acgaccagca tcttccatgg tcaagtagta gttacccaaa
    29221 accatatcct gagatggtgt tacgacaggt ttaccatctt tagggttaag gatgtgttct
    29281 gccgcaagca taaggatacg tgcttctgct tgtgcttctt ctgacaatgg aacgtgaatc
    29341 gccatctggt caccgtcgaa gtcggcgttg taggcttcac agacaagcgg gtgcaaacga
    29401 agggctttac cgtcgatcag aactggctca aaagcctgga tacccaaacg gtgaagggta
    29461 ggtgcgcggt tcaaaagaac tgggtgttct ttgatcactt cttccaagat atcccagata
    29521 cgatcatccc cacgttcaat caaacgtttt gcagctttta cgttaccagc aatatcacgg
    29581 gcaacgattt cgcgcataac aaacggtttg aagagctcga tagccatttc gcgtggcaca
    29641 ccacattggt acattttaag cgttggacca acagcgataa cggaacgtcc tgagaagtca
    29701 acacgcttac caagcaagtt ttgacggaaa cgtccttgct tacctttaag catgtggctg
    29761 agtgatttaa gtggacggct acctggtcct gtaattggac gaccacggcg accgttgtca
    29821 atcaaagcat ccacagcttc ttggagcatc cgtttttcgt tttgtacgat gatacctgga
    29881 gcgttaagtt ccaagagacg agccaaacgg ttgttacggt tgataacacg gcggtagagt
    29941 tcgttcaagt cagacgccgc aaaacggcca ccatccaact gaaccatcgg acgcaaatct
    30001 ggtggaataa ctggaaggat gttgagaatc atccactcag gcttgttacc agatttgtag
    30061 aatgcatcca atacatctaa gcgacgaact gctttaatgc gtttttgacc agaagctgtt
    30121 ttcaattctt ctttcaaagc tgcgatttct tttggaagat cgacttgttt caagaggtct
    30181 tggatggctt ctgcacccat tttagcaacg aatgaaccat aaccatattc acgcaaacgc
    30241 tcacggtatt cgcgctctgt catgattgac ttgtgctcaa gcggtgtatc tttaggatca
    30301 atcaccacgt aagccgcgaa gtagataact tcctcaagcg cacgtgggct catatccaag
    30361 gtcaagccca tacgacttgg aatacctttg aaataccaaa tgtgtgaaat tggtgctttc
    30421 aactcaatgt gtcccatacg ttcacgacgt acttttgcac gagtcacttc aacaccacag
    30481 cggtcacaag taatcccttt ataacggata cgtttgtatt taccgcatga acactcccag
    30541 tcttttgttg gaccgaagat gacttcgtca aaaagtccat cacgttctgg tttaagtgta
    30601 cgatagttga ttgtttcagg ttttttaacc tcaccgtaag accatgaacg aaccttactt
    30661 ggtgaagcta acgtgatttg catactttta aatcgattta cgtcaaccac taataatccc
    30721 tttcttttct tgtgagaggt tggattccca gacctcaaat tcttgaacaa taatcagcag
    30781 aatggatagc tggtaagcta tttttctata aatcagacgg tggaagaccg tcggccctta
    30841 tctatttcag ttctgaggtt tggaccaact tgttccatcc attctaatta ttctttgcct
    30901 tctgcttctt ctgctgcaaa ggctgcggct gcgtcagctg cggcttttgc acgtgctttt
    30961 tcaagatcat ctacgtggat gatgtcatca tcttcacctt catcaaggtc acgcaactct
    31021 acttcattgt tgtcttcatc aaggacacgc atatccaaac caagtgattg caattctttg
    31081 acaagaacgc ggaatgattc tggaacacct ggttttggaa ttggtttacc tttggtgatg
    31141 gcttcatagg ctttaagacg gcctgtcaca tcatctgact tataagtcaa gatttcttga
    31201 aggacgtttg aagcaccgta ggcttcaagg gcccaaacct ccatctcacc gaaacgctga
    31261 ccaccaaact gagccttacc tccgagtggc tgttgggtaa cgagtgagta tggtccgact
    31321 gagcgggcgt ggagtttatc atcaaccatg tggtgaagct tgatcatgta catgacaccg
    31381 acagatacac ggttgtcaaa tggctcacct gtacgtccat cgtaaagaat ggttttggca
    31441 tctgaatcca tacctgcttc tttaacagtt gaccaaagat cttctgaact tgcaccatcg
    31501 aaaactggtg ttgcgatatg gatgcccaag ttgcgagccg ccatacccaa gtgaagttcc
    31561 ataacctgac cgatgttcat acgtgatggc accccgagtg ggttcaacat gatgtcaact
    31621 ggtgttccgt ctggaagata tggcatatcc tcaacaggta caatacgtga aacgacaccc
    31681 ttgttaccgt gacgaccggc catcttatct ccgaccttga tcttacgttt ttgagcgatg
    31741 taaacacgaa caaacatgtt aacacctgat tgcaattcat caccgttggc acgagtaaag
    31801 attttcacat cacgaacgac accatcggca ccgtgaggta cacgaagaga ggtatcacgt
    31861 acttcacgtg acttgtcacc gaagattgcg tgcaagagac gctcttcagc agaaagatct
    31921 ttttcacctt ttggtgtaac tttaccaaca agaatgtcgc cctctttaac ttcggcacca
    31981 atacggataa tacccatttc gtccaagttg cgaagagcat cttcaccaac gtttggaatt
    32041 tcgcgagtga tttcttcagg gcctaacttg gtatcgcgtg tttctgattc gtattcttcc
    32101 aagtgaacag atgtatagac atcgtccttc acaaggcgtt cagacatgat aaccgcatcc
    32161 tcgaagttgt aaccttccca agtcatgtag gcaacgatag ggttttgacc aagagccatt
    32221 tccccatttt ccatagaagg gccatctgcg atgaagtcgc ctttttcaac gatgtcgccc
    32281 aattttacaa gggtacgttg gttgtaggca gtaccagagt ttgaacgacg gaatttagaa
    32341 atgtggtaga catcaagcga tccatcttca cgacgcactt ctaccttgtc cgcatctgcg
    32401 taaacaacct taccatcgtg ttgagcgata attgccgcac ctgagtcgtg agcagcctgg
    32461 tattccatac cagtaccaac gtaaggtgct tttggatcaa tcaatggaac agcctgacgt
    32521 tgcatgttgg cccccatgag ggcacggttg gagtcatcgt tttccaagaa aggaatacat
    32581 gctgtcgcaa cggcaactac ctgcttaggt gaaacgtcca tgaaatctgc tgaatctgct
    32641 gggaactctt ggttgttacc ttggtgacgt cccataacaa tcttatcaac gaaacggttg
    32701 ttttcatcaa gtggcgatgt tgattgggct acaatataag catcttcttc atcggctgtc
    32761 aaccaaacga tctcatttgt aactgtacca gttgcacggt caatcttgcg gtatggtgtt
    32821 tggatgaaac catacttgtt gagatgccca taagatgaca agttgttgat caaaccgatg
    32881 tttggtccct caggcgtttc aatcggacac atacgaccat agtgagtgta gtgaacgtct
    32941 cgaacctcat aaccagcacg gtcacgagtc aaaccaccag gtcccaaggc tgacaaacgg
    33001 cgtttgtgag acaactcaga aagtgggttg tgttggtcca tgaactgtga caactgagat
    33061 gaaccaaaga attctttgat tgcggctgta acaggacgga tattgatgat ttgttgtggc
    33121 gtcaatactt cattgtcttg aaccgacata cgctcacgca agttacgttc catacgagtc
    33181 aaaccgatac gtacttggtt agcaagcaat tcaccaacgg cacgaatacg acggttaccc
    33241 aagtggtcaa tatcatctac gcgaccaagt ccttcagcca agttgaggaa gtagctcatt
    33301 tcagccaaga tgtccgcagg tgttactgta cgtacgtttt ctgctggatt agcattacca
    33361 atcaccgtca caacacgttc tggatcctta ggagctacaa tcttgaattt ctgcaaaaga
    33421 actggctcaa gaagaacagc gttgtcattt ggaatatatt ctaccaagtt gagctcatcg
    33481 aattgtgctt ctactttttc aagcacatca cgactcaata cagtaccagc ttccaagacg
    33541 atttcgcctg tctcaccgtt aatcacatgc tcagcaagtg tttggttgag caaacgagta
    33601 cggaggttga gttttttatt aatcttgtaa cgaccaacag gtgccaaatc gtaacgacgt
    33661 gggtcaaaga aacgagctgt caaaaggcta cgagagcttt cagctgtctt tggttcacct
    33721 ggacgaaggc gctcatagat ttccttaagc gcttcatccg tacgagaatc tgctgggttt
    33781 ttatgaatat ctttttcaat ggtattgcga accaattcgc tatcaccgaa gatgtcaaag
    33841 atttcatcat cgcctgagaa accaagcgca cgaacaagcg ttgtgaacgg aatcttacgc
    33901 gtacggtcaa tacgagtgta ggcaatgtct tttgaatctg tttccaattc caaccaagct
    33961 ccacggttag ggataaccgt tgaaccataa cctactttac cgttcttatc tactttatca
    34021 ttgaagtaga cacctggtga acgaaccaac tgagaaacga tgatacgctc tgcaccgttg
    34081 atgatgaagg tacccatttc agtcatgatt ggaaactcac cgaagaagac ttcttgtgtc
    34141 ttgatctcgc cagtttcttt attcaccaga cggaaagtta catagattgg agctgagtag
    34201 ttcgcatcat gcgcacgcgc ctcttccaat gtgtatttag gctctttcaa ttcataacca
    34261 acgaattcca attccatggt atccgtaaag tttgaaactg gaagtacatc ttcaaagact
    34321 tctttcaaac cataatctaa aaagtcttgg aaagagtccg tttggatttc aaccaaattt
    34381 ggtaaatcaa gaacttcctt gattcttgaa aaactacgac gggtacggtg cttaccgtac
    34441 tgaacttcat gtcctgccaa aagttttctc ctttgtatta agatactaag ccgtaaacaa
    34501 ctcaaagaaa aataggaaac acgacgacgg aggcaagcct cctaggagct gtttatcttt
    34561 tttcacagag ttgtaggcga gttcaattaa aagatacgag accgtgaaaa ccaaagtgaa
    34621 aataagcggt aagtccaaaa tctttgattt tgttgacgca ccctcgtcag aacgactagg
    34681 actttcttga caaatcgtcg aagaaaggcc agacgtttac gacgttggga gtgacaaacc
    34741 aaagtaaata tttcatacac tttttcacac aggttttagc ccgagttcaa ttatatacag
    34801 ccatgtgatg accaacattc atcctttttc tgaattttta gaatctttaa gaatagataa
    34861 caattctcaa aaaaattcct catagacaca aaaagagcag ctaaatcttc ctttgaaaaa
    34921 gtttgattta actgctgcaa gccttgtttg gacaatattt caaacaagac acacgacaaa
    34981 taatttacac tattatagca caagtactag gaaaaatcaa ggtgaatcat tattcgcttg
    35041 ctcactagtt gctgtttgcg tttctgatga ctggttcgtg ttattagctg aagaactcgt
    35101 gctactccga gtcgaactgc ttcgttgggt tgagctactt gatgttggcg tttgggaacc
    35161 gataattgta ttccaagcct gtgctcggtc actatctgtc cccccaacca tgaagttata
    35221 gctcgttact ggtgcaccat ttttagccca ataactagtt gtcatcgcac caccaactgt
    35281 tacttggcgc ccatttacct gtgtcgtccc tgcacgttga cctgtagaag caacgacact
    35341 agaagcaata acactactgt ctaattcaaa ttttcctgcg aacatatccg cattaacatt
    35401 gtaaagcgca tcaaccatat gggccacata ttgtgaatta ttgtggtagc ctgtccagac
    35461 atacatttct gagttatcat cattcccagc ccaggtacct aaagttactc taggggttga
    35521 tagcatgagc cacacgtcat taacttcatt acttgtcccc gtcttaccga cgaaatcaga
    35581 tgatgctgcc tgtgggttta aaccactcaa tcgactcttg aacgtcgtag tataaccaga
    35641 gttgacaacc tgtctcaaaa gctggttcat aatacttgca gttgctttag aatagacctg
    35701 aaccggagcc gcttcatgct gatacactac tgttccatca gaggcagtaa tactctcaac
    35761 gatgtaatgt ttgttataaa cgccaccatt tgccaaagtt tgataggcat tggtatttgt
    35821 caatactgaa atttcaacac caccgcccaa cggtacactt tcaatatcgt acataggaat
    35881 atgataattc attttatcca tgtacgcttt ggcatctaca cctgcatttc tcatcatctt
    35941 ataggtccaa aaagcaggaa tgttaacaga acggtcaata gccgtctgta agttcatcat
    36001 accagtacca cgtcctgaac catacataat cggttgtcca cttgaaaagt tcgttggata
    36061 atcggaaagg atgctggcag aacctatcaa accttgatca atcgcaatac cgtaagctaa
    36121 cagaggcttg atggttgacg ctggcgaacg aagagtatca aaggcatggt tattttggtt
    36181 ggttgaataa tcgcgaccac ccacaaatcc taaaatggct cctgtggcat tatcaattaa
    36241 cacactacct gtctcgacat attcgtttcc atcatccaaa acagagccat aatttgccac
    36301 aacagactgc atcgccgcat agattccttg atcaacggta ctcttaatcg tgtagccacc
    36361 ttggctcaat tcttgcttgg ccaactcctc ataggatgcc tttgtttcat catttttcaa
    36421 gtcattttta gaaaccttat cgcgttttac caggtaatca aacataacct cctgcgcttc
    36481 ttccatgacc ttgtaataga gataatcttt cgtatccgcc attacaggag ctggagcaat
    36541 aaaatcttgt ttaatatcgt aggctttata ggtttcgtat tcttccttgg tcaagaatga
    36601 cgcccgatac atattaaaga gaacatcttg atagcgttca acaccgtaaa ccatatcttc
    36661 atctgattta cgtgttccat ctgatgcata gggagaataa acaatcggac tttgaggcaa
    36721 gcctgcaata aaagcagcct gcggaacagt caagtctttc gctggtttac caaaaattcc
    36781 ctgagccgca gcttctaccc ctgcaatatt tcgtccctga ttatttctac caaatggaga
    36841 aacatttaag taaatggtca aaatctcttc cttggtcata ttccgctcta atgccaaagc
    36901 cgaaacaatc tcattagcct tacgcgtaaa ggttggagca tctccgacaa gttgctgttt
    36961 tatcaattgc tgcgtcaagg ttgaaccacc gctagatgaa cctagaccaa ctgatcccag
    37021 tgccgcacgc aaaacagcct taggcacaac cccattgtgg gtttcaaatg tttcatcctc
    37081 tgtcgcaatt acagcctgtt ttagataatt cgagacctcc tccgaagtaa taggtacgcg
    37141 aagcagatct gaattaactt ccgacaccaa gctaccatcc gaataggtaa cggtggaaat
    37201 ccgactaact tctgaaacct tcgctaccag ttcctctgtt ttgggaatct taacgtcatc
    37261 aaaaagacta accacgaaac caattcctat gcccgcacca aaaagaccga ataggaacac
    37321 caagacagct actgaattga ataataattt cagcgtccga aggaaaactc ctactacatc
    37381 ccctaagact agggcactga ttttcttttt aaaatcttgc ttatctgatt tagtcgccat
    37441 attaactcct tatcgttcca ttataccaaa ttttgtttat tttccgcaat ttcgtggtat
    37501 aatagggagg aattttttaa aatgaagttc ggtccaaaaa tcatataaaa tggtttatgg
    37561 tcctgtatat taaacctaaa ggagacagcc cacatgaaca tttttgaaga actaaaagct
    37621 cgtggcttgg tctttcaaac gacagacgaa gaagccttgg taaaagcatt gacagaagga
    37681 caggtttctt attacacagg atacgatcca acagcagata gtttacactt ggggcacttg
    37741 gttgttatct taactagccg tcgcttgcag ttggcaggcc acaagcctta cgccctagtt
    37801 ggcggtgcaa ctggtctgat tggcgaccct tccttcaagg atgcggagcg tagcttgcaa
    37861 accaaagaca ccgtggacgg ctgggtaaca aaaatccaag gtcagctttc tcgcttcttg
    37921 gattttgaaa atggtgacaa caaggctgaa atggtcaaca actacgactg gttctcagac
    37981 atcagcttta tcgacttcct tcgtgatgtt ggtaaatact acacggtcaa ctacatgatg
    38041 agcaaagatt ctgtgaaaaa acggattgaa acagggattt cctacaccga gtttgcctac
    38101 caaatcatgc agggctatga cttctacgag cttaatgaca agtacaatgt gaccctacaa
    38161 atcggtggtt ctgaccagtg gggcaacatg acagccggta ctgaattgct tcgccgcaag
    38221 gctgacaagt ctggtcacgt gatgactgtt cccctcatca ctgactcaac aggtaagaaa
    38281 tttggtaagt ctgaaggcaa cgctgtttgg ttggatgccg acaagacttc tccgtatgaa
    38341 atgtaccaat tctggctcaa tgtcatggac gatgatgccg ttcgtttctt gaagattttt
    38401 actttcttgt cattagatga aatcgctgaa atcgagaaac aatttgacgc tgcccgtcat
    38461 gaacgcttgg ctcagaagat tttggctagg gaagtcgtga cattggttca cggtgaagag
    38521 gcttataacc aagcccttaa catcaccgag caattgttcg caggcaatat caaaaaccta
    38581 tccgcaaaag aactcaaaca gggcctcagc aacgttccaa actacgctgt acaggctgaa
    38641 gacaacctta acatcgtgga acttctagtt acctctggta ttgtcacttc aaaacgccaa
    38701 gctcgtgaag atgtgcaaaa tggtgccatt tatgtcaacg gtgagcgtgt gcaggacttg
    38761 gactataccc tttctgacag tgacaagatt gacggcgagc tgaccgttat ccgtcgtggg
    38821 aagaagaagt attctgtgtt gacttattaa gatttttcaa ggaaatccac catgatttcc
    38881 ttttgttcta tcaataggag aacagctatg acctgtattt tttgccatca gattgaagaa
    38941 atagatattc tctaccagac agaatatttc aaggtggtct gggacattga ccctattcaa
    39001 acagggcatc tactgattat cagcaaggaa cactatgaca cgctcagcca aatcccttct
    39061 gctgttcgct atgagctatc ggacttggaa gtctttttga ctggaaaact ctgtcaaaca
    39121 ttggcaattg acggtgtgac cacagcccgc aacgatcgct tgtttgatgc tggcacccat
    39181 ttccatgtcc acctgattcc acgctttcga tcagacggct tctgggacca aatttcactt
    39241 gcacaagtac agcttgacct gaacgccttt ctcaaagcat tataaaaagc agcccaacgg
    39301 ctgctttttc gcttatatag tcgtttagtt tacttttagt acaaggcaac gagccgcagg
    39361 cataactcaa tgctttcttg tttctaggag accagctgat gtacttcgct tttttgtttt
    39421 caggctcagg tacgaatagt ccaccggact attcgtatcc cccagactgt ttcaatcagg
    39481 caaggcgagt tcaaacactc cagtggagtg tttgaagttg gaaataagga aacgacgttt
    39541 cctcgctcgt cgaagtaata aaagagaaac taaatgacta taccaactct tccacttcat
    39601 aaatagtatc ttccagcacc tcttggtact gttccaagtc ataggctaca gtggtttcca
    39661 cctccgccaa ctgctcttcc agctggattt tcacctcttc caccccatcc aactccaaga
    39721 agtgattggt cacatgcttg acacagtttt ggcacgataa gtttttcaat ttcaaagttt
    39781 gcttcatgtt ttttctcctt ttgcatgatg cccacaggta cattgccccg ccaagcaccg
    39841 acaagcgatt tgctcgggtg cagtcctttg taactgcgag attttgctgg ccagtttttc
    39901 taggcttttt tgagaaaata tccctgtgtc tagcagtttt tcaaccaatt gcccctgttt
    39961 tgtggaacat atggtagcta gtaagagctc cacctgcccc tgcaaatgtt cctcttcact
    40021 gatcagcgga taatagtggt atttgctggt ttccttggcc attctcaaat agtttttctt
    40081 ccgcaaccgc cccaagagtg tcttaatggt cgtcgcctgc caatcaaagc cttcctgcaa
    40141 ggcctgaata atttcctgag aagtcgcccc aggttgcgcc caaagaaccc gcataacctg
    40201 ccactccgca gctgaaatcg tctgctccac actctcacct ccagccaatc agatttctaa
    40261 atttagttta cacttgtagt caaattctgt caagaagaaa gactgaacat cttgcccagt
    40321 cttactctct tagtgagcca acatttcctg accaatttcc aaaccagtca aaccaactgg
    40381 atagtaggtt ggccaatttt ccaattcttc caagagtttt tcttggcttt ctccaccgaa
    40441 atagatatgg aagtgacccg ttttaacagg tgcaatattg tggtcgctaa attgaacata
    40501 cttatagtta ccagcatttg catccgttgc ttcaaacaag aaacgaacac cacgatttcc
    40561 ttttttgtaa gtcaaaatct tatagcctac atacttatac gtgaattttt ctttcttatc
    40621 tcccactaca aattccatgg tattatctgt aatgttaatt tgatcaacat ctgttttgta
    40681 gccagtatcg taataagcct tgtactcttc agctgtcata ccacctttga tcttagcctt
    40741 gtaatcccag acctgatcta gtgtaccatc aagcaagtat ggataaacag attgccactc
    40801 acctgcataa tctgaaagag tacgatcttt tacggcacta tcttcaaaat aaccgttata
    40861 aacagtttgc gcagtctcag caccttcttc tggcaaaatt tcagaacctt cttgggaagt
    40921 tgttttttca agggcagtca gattatcttc cataacagaa atataatcct taccattttt
    40981 catatcctca tccgtcaaac tttcaagagg attaagaaca tccaactgaa ccccagtctc
    41041 tttagcaagt gtttctgcaa cagattttga cgcattttct tcaaaataga tatatttaat
    41101 accatattta ttaatatact ctgtcaattc tgctagacgt gatggagtcg gatcttcatc
    41161 cgcagcaaca cctgtaatag aaacttgttt caaaccatag tccaaagcca aatatgcaaa
    41221 tgcagtatgt tgtgtaacaa agtatttctg tttcgcagca gataatgttt cagaatattt
    41281 agcatccaaa gcatctaatt tctcaatata ggctgccgca tttgtttcaa aggcatcttt
    41341 cttttctggg tatttagcta ccaaactatc acgaatattt tctacaagtg tgatggcacg
    41401 ctcaggagac aaccatacat gcggatcata agcatggcta tgcctctccc cactatggtc
    41461 gtgaccttta tgttcctcct caccgccagg caaaagcaac atcccctccg tcgcactaat
    41521 cacattaacc tttgttgtgt caagtgattt ttcaacatca tgaacccatg tttccatatt
    41581 ctcattttca taaacaaaag catctgcctc ttggatacga gcaatatctt tagctgacgg
    41641 ctcataacca tgaacctctg tcccagcctt aaccaacaga tcaacatttg cctcatcacc
    41701 tgccacctgc ttggtgaact cataaacagg ataaaaggtc gttacaatat tcaacttccc
    41761 atcttcagaa gctgtgctat tgccacaagc ccccaaaagc aaagctgaga cagacaagaa
    41821 taataaacca acttttttca tcttaactcc ttatttttta aatttttgaa caacattcac
    41881 cagtaagaaa atactaatga aaatcaaggt aatacttgca ctcgctgggg tttctgcata
    41941 gtaagaagtc agcaacccca tgaccattcc aaaaaagcca atgcccatcc cagtaaagat
    42001 aacagcttta aaactcttac caagtcgcaa tgcaatagaa gcaggcaaaa ccataattgt
    42061 tgaaaccagc aaagcacctg ccgcaggaat cattaaggca atggcaacac cagtcaccac
    42121 attgaaggca atggacatag ctcgcacagg taacccatct acaaatgccg tatcctcgtc
    42181 aaaggtaaga atatacattg gacgtaaaaa cagcaatgtc agcacaatga caatcacagc
    42241 aatcgtaaac aaagcaatta cttgctcctg actaatggtc actatcgaac caaacagata
    42301 ttgttccaga ctaagccctg atttcccacc agatttattc atgacaatga gcgaaatagc
    42361 caaaccagtc gacatgagga tagccgtccc aatttccata aaattcttgt aaattgtccg
    42421 caaatactct aaaaatacag ctgccacaat aacgacaaga acagtcgaaa gcgtcggtga
    42481 aatccccaat accaaaccaa atgcaacccc cgccaacgaa acatgactaa gagtatccga
    42541 catcaaactc tgccgtctaa gtatcaaaaa gacccccaaa atgggcgaga acagactcat
    42601 cgcaatgatt gccaggaaag cacgctgcat aaagtcataa tgaaatacag atagatcaaa
    42661 catgggcaac ctcctcatcc gcttcatgaa cattgaaaca acgccaagga gactgctgat
    42721 cacgaaccaa gtgaatattt cgatcagcat acttattcag ctcatcagga tcatgagtta
    42781 tcatcaaaac cgactttcca tgcttcttag ccgaatggtg catgagctgg taaaaagcat
    42841 ccttggtccc tgcatccatc cctgttgtcg gttcatccag aataaaaata tctggatcag
    42901 aggcaaacat acgcgcaatg accgcacgct gcttctgccc accactcaaa gcacctaagc
    42961 gcttatcccg atgttcccac attccaactg attccaaact aatccgaaca tgttcctcat
    43021 catgggctgt caaacgacga aaccaacctt gtcgaggata acgccctgat tttacaaatt
    43081 cgtaaaccgt gctcggaaaa ccagcattaa aacttgcaat ttgctgaggt aaataggcca
    43141 ttcttagctt cttacccttt atactcttct cagcaataga aaccttccct tgtttgggct
    43201 tcaatatccc caaagtcgcc ttaatgaggg ttgtcttggc tgcgccattc tcaccagtca
    43261 aagtcacaaa ttccccacta tcaagataat aatgaatatg ttctaaaacc ggttctttgt
    43321 cgtagtaaaa cgacaagtct tccacagtaa tatatctcat ctattttcta atcttctcca
    43381 ctaactcact taagaatcga gcaattgact cctgctcttc aagactataa tgttccaaaa
    43441 gctcttcata agcttccaag gtatgcgcgt gatgatgagc atgctctgta gcaatcggct
    43501 tagcagcctc agtcaaacta aagcgaacaa tacgagcgtc cttatcatct ctaaccgcca
    43561 ccaataaccc ctgagcaact aatgactttg tagccttcgt gatcgcagcc tgactaacat
    43621 tcaactcctt agcaatctca gtattagtat atgcagcctt ttcaataagc atcaaaatat
    43681 gctcctgcgt attcgtcaac ttcaccgtgc tttgacaaga accaaccaaa atctccaatt
    43741 gattctccga actcaaaaca atctcgtgca ggtacttctc aatctctaat acaatacgat
    43801 tcataggcat ccccttaacc ttttaattaa cttgttaagt ataccacata gaaaacaaaa
    43861 aaacaaggaa aatatttgaa agctaaattc ccaaagtaag ttctagtctg actcgaaaac
    43921 tagaaactag actgagacga aaaaataagg tagaacttga gaacaataaa gtacgtttag
    43981 aattaaagag tttagttgaa aatggtaaat aaaaattact ctttacagtc ctattcttta
    44041 caggtctaag acttagagaa gcactcgcac taacacggca agatatcgac ctttcgagca
    44101 atacaattca tatcacaaag tctgtatacg ttaataaagg cataagccat atcagtgcaa
    44161 cttaaactaa agcaggaact agaagaaata tcatcaataa aaaattaagt caagaactgc
    44221 aatattggta acagcaacaa aaacatctat tagagcagtt tacaagtgat agcatgagcc
    44281 tacaagtgtt tcaaagtaat cccattacga ttacaaaaat tcaatagaaa gcagtacaaa
    44341 aaaattcttg atcgtgatgc ggctcttaaa aagatacgca tacacgattt taagcactca
    44401 cacgcttctc tattgataaa tcgaggggaa gattatttag ttgtgaaaga aaggctagga
    44461 cacgcttcta taacaactac aatcgatacc tattctcact tgtatccaaa caagcaaaaa
    44521 gatttagctg ataaattaga tgac
//
LOCUS       DAZQCJ010000005        38902 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000005 DAZQCJ010000000
VERSION     DAZQCJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38902
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.005"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            86..451
                     /locus_tag="ACHU66_000279"
     CDS             86..451
                     /locus_tag="ACHU66_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFR3388594.1"
                     /translation="MTVKINTKDGQIELTDDVIATVVGGATTEIFGVVGMASKSAIKD
                     NFQALLRKENYSKGVVIKTLEDGRIAVDVYTVMSYGVKISEVSRNIQERVKFNLENQL
                     GISVDAVNVFIQNIKVVGE"
     gene            454..2118
                     /locus_tag="ACHU66_000280"
     CDS             454..2118
                     /locus_tag="ACHU66_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFR3388595.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTITNGAKEVADKSASTVGEVAQILSKGLLMGARGNSGVITSQLFRGFGQSVKGKVE
                     LDGKDLAQAFQHGVEVAYKAVMKPVEGTILTVSRGAATAALKKAEETDDAVEVMRATL
                     DGANRALKKTPEMLPVLKEVGVVDSGGQGLVYIYEGFLSALTGEYIASEDFEATPAVM
                     SEMINAEHHKSVAGHVATEDITFGYCTEIMVALRQGPTYVKDFDYEEFRNYLNDLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQAGLQYGSLVKVKVDNMREQHEAQVEKVESFQKPA
                     EQKEYAIIAVAAGEGLTEIFKSQGVDYVISGGQTMNPSTEDFVNAIELVNARNVILLP
                     NNKNILMAAQTAAEVAEVAVKVVETKTLPQGFTSLLAFDPSRDLESNFEAMTASLAEV
                     KSGSVTTAVRDTTIDGLEIHENDNLGMVDGKIVVSNPDMMETLVATFDKMLDEDSEIV
                     TIYIGEDGDEELAQTLAEQLEEQFEDVEVEIHQGNQPVYPYLFSVE"
     gene            2219..2659
                     /gene="tsaE"
                     /locus_tag="ACHU66_000281"
     CDS             2219..2659
                     /gene="tsaE"
                     /locus_tag="ACHU66_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR3388596.1"
                     /translation="MYSQNENELIAIGERIGKACKPNQVLVLSGDLGAGKTTLTKGLA
                     KGLKIEQMIKSPTYTIVREYEGTMPLYHLDVYRIGDDPDSIDLDDFLYGGGLTVIEWG
                     ELLDVSLFDDYLLIRIEKEGDGRRLTVESHGAQSSDLAKEFQNV"
     gene            2652..3179
                     /locus_tag="ACHU66_000282"
     CDS             2652..3179
                     /locus_tag="ACHU66_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFR3388597.1"
                     /translation="MSEKEVYFSEAEPADAAAFIDFMNQVARETDYLVMDETGFRFSV
                     DEMETIFEAGIENPRELCLLAKVGSEVVGAISVKSSKQFRISHIGNIFIAVKKAYWGH
                     GLGTILLEEVLHWAEEMDVLKRLELTVQVRNQAAVHLYQKLGFNIEGTQIRGARTDEG
                     EWLDLYYMGKLIGEV"
     gene            3176..4369
                     /locus_tag="ACHU66_000283"
     CDS             3176..4369
                     /locus_tag="ACHU66_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFR3388598.1"
                     /translation="MTIGKKIFLMSLAIIGLTVGAGLIYGASLLNFSTDAISKTFKQL
                     DGEEKITPIDATEPLTILLMGVDMDQATRGGDWEGGRSDSMILVTVNPKTKETNMMSL
                     TRDIMVEIAEANGESSGTVEKLNHSYSYGQAPMAIATIEKMMDINIDRYIEINMDGLV
                     ELVDAVGGIEVNNTLGFPISISEHEPAYTSIVQPGKQLVNGDQALVYARMRYDDPEGD
                     IGRQRRQREVITAIIKKLLQLDGFTQYKKILTAISNNMRTNIEISPATIPNLLGYKDS
                     ISKLNSYQLRGVDQMVDAIYYQLPTSTHLLEMQNVLKKSIGLEENTDLVTNVKVYEGQ
                     MGLPSPINVYDVYTNLLLLEASPWAESLDPEIGSPSATTTVTTEEQVTDFSTEPVLGD
                     ENGSQ"
     gene            complement(4478..4780)
                     /locus_tag="ACHU66_000284"
     CDS             complement(4478..4780)
                     /locus_tag="ACHU66_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HFR3388599.1"
                     /translation="MKLRNIILAVSAATASFLAVTHRDKIAKEVQETKQLLSDIQLNK
                     DNIQEQLAIIQSFQEPLQEMASDLQYKTRVYQQSIAGNLEEIQQILKKYQNENNTP"
     gene            complement(4782..5195)
                     /locus_tag="ACHU66_000285"
     CDS             complement(4782..5195)
                     /locus_tag="ACHU66_000285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFR3388600.1"
                     /translation="MSDCIFCKIISGEIPASKVYEDDQVLAFLDITQATKGHTLVVPK
                     KHYRNVLDMDKEAAATLFSVVPTIARQLKEKLGASGLNIVNNNEEAAGQTVFHTHIHL
                     LPRFNNQDGLSIQFKANDPDFPALAQLAQELYLGE"
     gene            5258..5992
                     /locus_tag="ACHU66_000286"
     CDS             5258..5992
                     /locus_tag="ACHU66_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3388601.1"
                     /translation="MLEVKNVTGGYVNIPVLKDVSFTVEDGQLVGLIGLNGAGKSTTI
                     KEIIGLLTPYQGQIAIDGLTLLQGAENYRKKIGFIPETPSLYEELTLKEHLEVVAMAY
                     DLSWEQAWGRVEHLLKLFRLDEKLDWFPVNFSKGMKQKVMIICAFMVEPSLLIVDEPF
                     LGLDPVAISDLIALLGEEKSKGTSILMSTHVLDSAEKMCDSFVILHQGQVRATGNLEE
                     LQSAFGMDGASLNEIYLALTQEGVAG"
     gene            5989..7029
                     /locus_tag="ACHU66_000287"
     CDS             5989..7029
                     /locus_tag="ACHU66_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR3388602.1"
                     /translation="MKELFQQRRLDFLNRCSKYLRYVLNDHFVLVLMVLLGFLSLQYR
                     QLLLSFPENPLPIVFVVIAISLLVLVSGRIATYLEEADQIFLLTKEKDILQGLEVAAR
                     CTVLFWSSIQVVVQLVLLPIYLKLGLPVWMIVAGLLTLLVLKCLFVKRQLVAYQSQGV
                     LDWSKAIQDEQKRKQSILRFFALFTTVKGISTTVKPRSYLNGILRLVKSRQTWFYLYL
                     RAFLRSGDYLGLTIRLLLLAILSLFAIEESWLSIGLVLVFHYLLVFQLLGLYKHYDYQ
                     YITQLYPLEKDSKKIGLQNLLRVILYSLLLVELGFALLFSKENTMVVVLLLVGIFLHE
                     IYLPLKLKKLID"
     gene            7079..7873
                     /gene="ccrZ"
                     /locus_tag="ACHU66_000288"
     CDS             7079..7873
                     /gene="ccrZ"
                     /locus_tag="ACHU66_000288"
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle regulator CcrZ"
                     /protein_id="HFR3388603.1"
                     /translation="MQFDTSGLQLQSIAGNSGKAFKGLRSDGTPVFVKYEMPPIVSAL
                     AREQITPPVLSANREVGMGHRVEQEWLNGRTLERQDMTSKQIRQILVRMHYSRILLNQ
                     ALQMNYTYMEPQDLVYRWQEEAPTRLGQNSYLQSICQDLLNNLPRFRQEVATFVHGDL
                     HHSNWVETTSGLVYLTDWETACVTDRMLDVAYILTHYIPRQSWEEWLRAYGYKYNSTV
                     LNKVYWYGQLGYLNQIAKHVESYNVLAANKEIYALRQFRQSFYKEV"
     gene            7870..8508
                     /gene="trmB"
                     /locus_tag="ACHU66_000289"
     CDS             7870..8508
                     /gene="trmB"
                     /locus_tag="ACHU66_000289"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR3388604.1"
                     /translation="MRVRNRKGASELLANNPQYVISNPEECKGKWAEIFGNNNPIHIE
                     VGSGKGRFVTGMAAQNPDINYIGIDIQMTVLSYALDRVLEAGLPNIKLLQVDGSSLTN
                     YFAPAEIDQLYLNFSDPWPKKRHEKRRLTYKSFLDTYKEILPEKGEIHFKTDNRGLFE
                     YSLASFSQYGMVLKQVWLDLHADGLEGNVMTEYEEKFSNKGQVIYRVEAQFQ"
     gene            8766..9236
                     /gene="rimP"
                     /locus_tag="ACHU66_000290"
     CDS             8766..9236
                     /gene="rimP"
                     /locus_tag="ACHU66_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR3388605.1"
                     /translation="MSSIIELVTAAITPAIQTPYELVDVEYGKMGGDYVLSIFVDKEG
                     GISLQDTADLSEKISPILDTIKPDPFPDQYMLEVTSPGLERPLKTADAVEKAVGKYIH
                     VKLYQAIDKIKVFEGTLLSFDGTDLIMEYMDKTRKKEVTIPYQTVAKARLAVKL"
     gene            9309..10451
                     /gene="nusA"
                     /locus_tag="ACHU66_000291"
     CDS             9309..10451
                     /gene="nusA"
                     /locus_tag="ACHU66_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR3388606.1"
                     /translation="MSKEMLEAFRILEEDMGINKADIIDAVTESLRSAYKRRYGQSES
                     AVIEFDEKKGDFHVFTVREVVDEVFDSRLEISLKDALAISSAYEMGDKIKFQEDPAEF
                     GRVAAQSAKQTIMEKMRKQKRAITFNTYKQHENEIMSGTVERFDNRFIYVNLGTIEAQ
                     LSKQDQIPGEVFQSHDRIEVYVYKVEDNGRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGSNIKKITSKFHPARYDAKNDR
                     MIPTEENIDVIEWVADEAEFIYNALAPAEVDQVLFDTEDGKHATVVVPDDKLSLAIGR
                     RGQNVRLAAHLTGFRIDIKSASEYEAFEATQYATDEVVEEVAEEQE"
     gene            10468..10764
                     /locus_tag="ACHU66_000292"
     CDS             10468..10764
                     /locus_tag="ACHU66_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFR3388607.1"
                     /translation="MAKARKIPLRKSVVSGEIIDKRDLLRIVKNKEGQVFIDPTGKAN
                     GRGAYIKLDNDEASQAKKRRVFDRSFSMEVAEEFYDELIAYVDHKIKRRELGLE"
     gene            10721..11059
                     /locus_tag="ACHU66_000293"
     CDS             10721..11059
                     /locus_tag="ACHU66_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFR3388608.1"
                     /translation="MLIIRSRGENLDSNKRQKILNLLGLAQRAGRLVSGEDLVVEAIQ
                     KGQAKLVFLAEDAAGNLSKKVTDKSHTYQVEVVTVFSTLELSAAVGKARKVLAVTDAG
                     FTKKMRSIME"
     gene            11077..13899
                     /gene="infB"
                     /locus_tag="ACHU66_000294"
     CDS             11077..13899
                     /gene="infB"
                     /locus_tag="ACHU66_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR3388609.1"
                     /translation="MSKKRLNEIARELGVSSKEVVAKAQELGFEVKSHASSVDEASAK
                     RLAESFGGQKSEATKVAAKVSKPEKVDETPKVETAKVEKAKETQPVVKEEVAASAVQS
                     VPHRPQSRNFKAEREARAKEQAAKRAQGQGKGGQAKSGQDRRDNRQQGQGRSNNERND
                     RRDNRRDQRPEERKDNRFGERRDNRANRRQDNRSGRSARFEQREAAKPAGPKIDFKAR
                     AAALKAEQNAEYARTSEERFRQAQEAKKQPKKAKEIKFEEPVVESKPFVKPAPVASVP
                     EQVAETTVDTRRKKQARPDKKRYFNSDEEDGPRKQQRNRNSQNQVRNQRTSNWNNNKK
                     NKKGKANQPAKPVTERKFHELPTEFEYTAGMTVAEIAKRIKREPAEIVKKLFLMGVMA
                     TQNQSLDGDTIELLMVDYGIEAKEKVEVDNADIERFFVEEGYLNEEEMTERPPVVTIM
                     GHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIEEAGKKITFLDTPGHAAFTSMR
                     ARGASVTDLTILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGE
                     LAEHGVISTAWGGESEFVEISAKFNQNIDELLETVLLVAEIQELKADPTVRAIGTVIE
                     AHLDKGKGAVATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKTAGPSTPISIT
                     GLNEAPMAGDHFAVYEDEKSARAAGEERAKRALLKQRQATQRVSLENLFDTLKAGEVK
                     SVNVIIKADVQGSVEALASSLQKIEVEGVRVNIVHSAVGAINESDITLAEASNALVIG
                     FNVRPTAEARSQAEADDVEVRLHSIIYKVIEEMEDAMKGMLDPEYEEKIIGEAIIRET
                     FKVSKVGTIGGFMVVRGKVTRDSSVRVIRDGVVVFDGKLVSLKRYKDDVKEVGNAQEG
                     GLMIENYNDLKVDDTIEAYIMEEIKK"
     gene            14055..14408
                     /gene="rbfA"
                     /locus_tag="ACHU66_000295"
     CDS             14055..14408
                     /gene="rbfA"
                     /locus_tag="ACHU66_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR3388610.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMVGD
                     LSMAKVYYTIMSNLASDNQKAQTGLEKATGTIKRELGRKLTLYKIPDLVFEKDQSIEY
                     GNKIDQMLRALDQKD"
     gene            14690..15361
                     /locus_tag="ACHU66_000296"
     CDS             14690..15361
                     /locus_tag="ACHU66_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin lysis protein"
                     /protein_id="HFR3388611.1"
                     /translation="MKTFISKLMLVSTVVLLAACQSNQVKGGESSSSSASSQVSDGSS
                     LQQASTIASSSETQASSVTTPELSEVYQSVIDRYQANMGQSAEAINQDEVSSYLSLLT
                     SQGQEYSGIFYSLYDVNHDGTEELLLALDKSGEYVLIDLYTQLADESLRLVDNFRNLG
                     FEIGPNALLRPLQDGTYLFEGDGIFRIYQYNAMIPGLKRISESDTNPETSPLLELKSL
                     HWVKL"
     gene            15530..16597
                     /locus_tag="ACHU66_000297"
     CDS             15530..16597
                     /locus_tag="ACHU66_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HFR3388612.1"
                     /translation="MKTNLKKLLYSGVTLMSLGVLAACSSTSSSTTTSSSAATSQTSV
                     ATTSNNSTSSDSSSSTIDWSALPTTEVTLSNDGLKITEGGTYILTGSTTAGVTVETDA
                     NVRIILAGAEISSSDTAAINVINADNVELEIKDGTTNTVKDTSNHTDTNIEGAIHVEA
                     DLTITGNGSLTVEGNFQDGIVSTDDLVVNSGNITVTAVDDGIRGKDSLTINGGEITVT
                     AGDDGIKAETALTIDGGTITVSESAEALEGTNITINGGTLDVYGSDDAINAASTTSSE
                     IFIKVTGGDLKVAVGSGDTDAFDANGDIYISGGTIDVTAQSAFDFDGTAELTGGTVTV
                     NGEQITQITATGPGAGGHGGW"
     gene            complement(16633..17292)
                     /locus_tag="ACHU66_000298"
     CDS             complement(16633..17292)
                     /locus_tag="ACHU66_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR3388613.1"
                     /translation="MEFDLFLMICNYIGTIAFAVSGAVKGFKKKLDIFGISLLGIITA
                     VGGGIIRDTMANRIPAALTDPTAIYLSLGVSISMYLIVINLKQDKPLDKKMIHFLSQT
                     NLIFDAIGLAIFALIGASTGISLQLNALTSGILAALTGVGGGIARDLLVNETPIVLKE
                     DVYAVLAFFSGVLYHLCVVNWKLPQIPTFITIFTISLIIRLLVIKYKINLPNMESKRK
                     V"
     gene            17525..18493
                     /locus_tag="ACHU66_000299"
     CDS             17525..18493
                     /locus_tag="ACHU66_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HFR3388614.1"
                     /translation="MVSITKEQHLDMFLKMQQIRDVDMKLNKLVRRGFVQGMTHFSVG
                     EEAAAVGPIAGLTDEDIIFSHHRGHGHVIAKGIDINGMMAELAGKATGTSKGRGGSMH
                     LANVEKGNFGSNGIVGGGYALAVGAALTQQYLGTDNIVIAFSGDSATNEGSFHESMNL
                     AAVWNLPVIFFITNNRYGISTDISYSTKIPHLYQRAAAYGIPGHYVEDGNDVIAVYEK
                     MQEVIEYVRAGNGPAMVEVESYRWFGHSTADAGVYRTKEEVNEWKAKDPLKKYRKYLT
                     ENKIATDEELDAIEAQVAEQVEASVKFAQESPDPDISVAYEDVFVD"
     gene            18780..19775
                     /locus_tag="ACHU66_000300"
     CDS             18780..19775
                     /locus_tag="ACHU66_000300"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFR3388615.1"
                     /translation="MAETKVMALREAINLAQSEEMRKDEKVFLMGEDVGIYGGDFGTS
                     VGMLDEFGPKRVRDTPISEAAIAGSAVGAAQTGLRPIVDLTFMDFITIALDAIVNQAA
                     KTNYMFGGGLKTPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGIKVVAPGTANDAKGL
                     LKSSILDNNPVIFLEPKALYGKKEEVNLDPDFYIPLGKGEIKREGTDVTIISYGRMLE
                     RALKAAEEVAAEGISVEVVDPRTLIPLDKELIIESVKKTGKVILVNDAYKTGGFIGEI
                     ASIITESEAFDYLDAPIIRIASDDVPVPYANILENAVLPNVEKIKAAIYKQVNKG"
     gene            20115..21503
                     /locus_tag="ACHU66_000301"
     CDS             20115..21503
                     /locus_tag="ACHU66_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HFR3388616.1"
                     /translation="MAIEIIMPKLGVDMQEGEIIEWKKQEGDFVNEGDVILEMMSDKT
                     SMELEAEESGVLLKIVHGNGATVPVTEVIAYIGAEGETVEAGASSAPAVEPAAAIEEV
                     PAGRTPVIVAPATAAKPQGSGKVRATPAARKLARELGIDLGLVPGTGANGRVHKVDVE
                     DFKGAAPKATPLAARIAADQGVDLSTLTGSGVNGKIVKNDVLAVLAPAAVETAAPAPK
                     AEEKPAKELPEGVEIIKMSPMRKAISKGMVNSYLTAPTFTLNYDIDMTNLMALRKQVL
                     EPIMNKTGLKVTFTDLIGLAVVRTLMKEEHRYMNASLINDAQEIELHKFVNLGIAVGL
                     DDGLVVPVVHGADKMSLSDFVVASKDVIKKAQSGKLKGAEMSGSTFSITNLGMFGTKT
                     FNPIINQPNSAILGVASTVQTPVVIDGEIKIRPIMALCLTIDHRIIDGMNGAKFMVDL
                     KNLLENPLELLI"
     gene            21777..23537
                     /gene="lpdA"
                     /locus_tag="ACHU66_000302"
     CDS             21777..23537
                     /gene="lpdA"
                     /locus_tag="ACHU66_000302"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR3388617.1"
                     /translation="MAIEIIMPKLGVDMQEGEIIEWKKQEGDFVNEGDVILEMMSDKT
                     SMELEAEESGVLLKIVHGNGTTVPVTEVIAYLGAEGESVEVGSAPAPAEVAQATADLK
                     AAGLEVPAAPAAAPQAPKAELAADEYDMVVVGGGPAGYYAAIRGAQLGGKIAIVEKSE
                     FGGTCLNKGCIPTKTYLKNAEILDGLKIAAERGINLASTNYTVDMDKTVDFKNKVVKT
                     LTGGVQGLLKANKVTIFNGLGQVNPDKTVVIGDKVIKGRSIILATGSKVSRINIPGID
                     SKLVLTSDDILDLREIPKSLTVMGGGVVGVELGLVYASYGTEVTVVEMADRIIPGMDR
                     EVSVELQKVLSKKGMKFLTSVGVSEIVEANNQLTIKLNDGSEIISEKALLSIGRVPQL
                     AGLENLHLELDRGRIKVNAYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAIHG
                     NHHKAKLDFTPAAVYTHPEIAMVGLTEDQAIEKYGKENILIGRNSFTGNGRAIASNEA
                     HGFVKVIADKKYHEILGVHIIGPVAAEMINEAATIMESELTVDDVAASIHGHPTFSEV
                     MYEAFLDVLGVAIHNPPKRK"
     gene            23773..24618
                     /locus_tag="ACHU66_000303"
     CDS             23773..24618
                     /locus_tag="ACHU66_000303"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFR3388618.1"
                     /translation="MRKIIFLDVDGTLVDYHNRIPESAIRAIRQARENGHLVYVCTGR
                     SRAEMQPELWEIGLDGMIGGNGSYVEHQGQVIMHQLLSEEDSRAIVDWLHERGLEFYL
                     ESNNGLFASENFRERARETLRIYSMNKGKTAEEVATQEVDDVIHGMIFDSQLYRNDLN
                     KVSFVLDSYQDHLDSKEAFPQLVANTWGGRGESALFGDLGVKDIDKAHAISVLLDHLG
                     ASQADTIAFGDAKIDISMLDYCAVGVAMGNGGAEILAMADMITDDVEEDGLYNAFERL
                     GLLDK"
     gene            24785..25618
                     /gene="licT"
                     /locus_tag="ACHU66_000304"
     CDS             24785..25618
                     /gene="licT"
                     /locus_tag="ACHU66_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFR3388619.1"
                     /translation="MQIEKVYNNNVIQAIDQQGRELIVMGKGLGFQKKAGEELDTSKI
                     EKTFVLQNDYQQSDLSSLYLQMESGEVEVVNAIINRAEETLEVQFDLSLYLALADHLH
                     FVFQRCREGIFIENPLSWEVRKFYPKEYQIGLEALQLVQERLGLELGKGEASSIALHL
                     VNAQKNGAFGKETQTISKIVTQILDIVRLHFGSVTYEEDTSYHRFVTHIQYFAQRVAN
                     GVVEGVNDAFLYEQVMANYPDSFACTTKIRQHIAEVYHFEMSKDEQVYLTIHIQRLKT
                     S"
     gene            25818..27728
                     /locus_tag="ACHU66_000305"
     CDS             25818..27728
                     /locus_tag="ACHU66_000305"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFR3388620.1"
                     /translation="MAKDYTDLATDIVAHVGGKENISSLKHCVTRLRFGLKDESKADT
                     DYLKARDGVVTVVQAGGQYQVVIGNHVPDVYAAVQKVAGISGDGALDIDEGDGPKGNL
                     FDRFVDLVSGVFQPSLGVLAAAGIIKGLVSILAATMGLNNDNSALYAILNTAGDGFFQ
                     FLPIVLAFNAARKFRMNEFTGMAIAMALVYPTLPGTFATLKEAGLNNVFGIPFVLPAA
                     GSYLQTVMPVLLAVWIASHIEKFMKKITPDVVKVFIVPFVTILISVPLTFLVVGPVAN
                     YASDLLSAGFTAVMGFSPLLYGILLGALWQVMVMFGLHWALVPLAILQFSQNGWSNIL
                     LAAALPNFTQTGVLLAIMMKTKESKVKSVAMPAFISSIFGVTEPAIYGVTLPMKTPFY
                     ISCAVSAVIGAGLSFFDLKNYAMGALGIFMYPGYVSPELGVTPMWIMMGFSLAAIVLS
                     FAIQMALPVPYLYGEPTEKKSVEETKESVPELKEIKQEIIASPLIGKVVKLEDVPDAV
                     FASGAMGKGIAIDPLDGILVSPAKAEVTLLFPTKHAIGLRTENGAELLIHIGMDTVSL
                     AGKGFESFVQVGDQVEAGQKLLEFDLHQIKAADLPVITPIIVTNTADYEDILVTQESQ
                     INSGDYLLTTVK"
     gene            complement(27773..28102)
                     /locus_tag="ACHU66_000306"
     CDS             complement(27773..28102)
                     /locus_tag="ACHU66_000306"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR3388621.1"
                     /translation="MITMHLYYTGPADNARAFAQEMEASGIADRIRQQAGNTRYQYFA
                     PLDDPHSILLVDSWTDQAALDIHHASPMMQEILDLRSKYQLTVTAERYKSDEAGIPEQ
                     DKDFLQR"
     gene            complement(28352..29266)
                     /locus_tag="ACHU66_000307"
     CDS             complement(28352..29266)
                     /locus_tag="ACHU66_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR3388622.1"
                     /translation="MQPPLTDKQFKHTIDYDSRLLPYKYYNTYVTDGIPDILFHWHNE
                     FEINYVYGGTARYHIDYDYFNSQAGDIILIRPNAAHSIHPIEQRGHETDTLLFHLDML
                     GASHLDQTSMLYLQPLQTDVFKCIPRIQPQDPGYAEIRQCLLTIFELVRHQPPYFELP
                     LKSRLFDFFYLLYRYRYIVRKNTDDMYRKNEKLRLLIEYIQQHYAENITIEQLAEQMG
                     YTKTHFMTIFKQQTGTSCMEFIIQVRLRAATELLTNTIKPVLEIATDVGFNNLSNFNR
                     QFKQYYHTTPSSYRKQLRKNRTCTNFDK"
     gene            29377..30858
                     /locus_tag="ACHU66_000308"
     CDS             29377..30858
                     /locus_tag="ACHU66_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFR3388623.1"
                     /translation="MSRFPKNFLWGGAIAANQAEGAYNVDGRGLVQTDVTTGGSVNSP
                     RYTTYIDKDGKPGKYASMGHAGGHLPEGATYAVLEDHYYPNHKAVDFYHRYKEDIKLF
                     AEMGYSVFRLSISWARIFPNGDDAEPNQAGLDFYRSVFEECRKYGIEPLVSIWHFDTP
                     LSLEQRYGGWTNRKLIDFYVRYAETIFNEYKGLVKYWITFNEINNTIMFLDMFSEPED
                     SDYQEAYQHLHHQFVASAKAVKLGHQIDPSYRFGNMICGITFYPGTCDPADILENQHK
                     WEQNIFYCSDAQAKGRYGSYANRLWKEHNVVLQMEEGDLEILAEGKVDMYTFSYYMST
                     VVTTHKVEDTVSGNFSIGAKNPYLTYSDWGWAQDPTGLQYYLEKIYDRYEIPMMIVEN
                     GLGALDTVEEDGSIHDDYRIDYHRAHVNAMADAIANGVDLIGYTTWGCIDLVSAGTGE
                     MRKRYGFIYVDMDDAGNGTLARTPKDSFYWYKKVIASNGEDVE"
     gene            31096..31479
                     /locus_tag="ACHU66_000309"
     CDS             31096..31479
                     /locus_tag="ACHU66_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002962841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR3388624.1"
                     /translation="MKIEHVAIYTQDLEGMRNFFENYFNATSNQLYHNLKTSFKSYFL
                     TFEDGVRLEIMTRDDVVDKPSQLNYLGLIHLAFSLGSEEAVDELTERLVAAGYLLLSG
                     PRITGDGYYESCVLGFDDIQIELTV"
     gene            31775..33208
                     /locus_tag="ACHU66_000310"
     CDS             31775..33208
                     /locus_tag="ACHU66_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR3388625.1"
                     /translation="MSKLPKNFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVP
                     REITAGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEAGLQFYDNLFDECLKYGIEPVVTLSHFELPYHLVTEYGGFRNRKTIEFFVRFAETC
                     FRRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYQEGENREAVMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCKPSDILMAQKAMQKRYYFADVHVHGFYP
                     EHILRYWERKGISVDFTEEDREDLLEGTVDYIGFSYYMSFAIDSHRENNPHYDYLETE
                     DLVKNTYVKASDWEWQIDPEGLRYALNWFTDMYHLPLFIVENGFGAIDQVEEDGMVHD
                     DYRIDYLGAHIKEFIKAVDEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDE
                     GNGSYARSPKRSFAWYKNVITTNGEEV"
     gene            33289..33528
                     /locus_tag="ACHU66_000311"
     CDS             33289..33528
                     /locus_tag="ACHU66_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HFR3388626.1"
                     /translation="MVGFIAACLTTFGFVPQVIKVVKTKDTESISLGMYMMSVAGMSL
                     WLFHGIIQGDTALIIANSLSVTLAGIILVYKLVYK"
     gene            33752..34090
                     /locus_tag="ACHU66_000312"
     CDS             33752..34090
                     /locus_tag="ACHU66_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388627.1"
                     /translation="MESIVGIVVIVVVTVVLVYLLTNMWKIRAVSVGQTDDTTLYFVR
                     FKGVNPPDLANSSVFNQVAKIKCAKGDTYQVTSTVNDVKLRDVIMDEFQLKSSEVVVY
                     YPPRLFFYAS"
     gene            34268..34582
                     /locus_tag="ACHU66_000313"
     CDS             34268..34582
                     /locus_tag="ACHU66_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFR3388628.1"
                     /translation="MVKALIICAGGMSSSLIAKKTQTLLEEQGHKVEMNAVGVPEGGK
                     KINAAEYDLYLISPQTKMHFKQFEEAGKKVGKPIVQIPPQAYIPIPMGIEKMAKLVLD
                     NI"
     gene            34629..36602
                     /locus_tag="ACHU66_000314"
     CDS             34629..36602
                     /locus_tag="ACHU66_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFR3388629.1"
                     /translation="MLSTKEKMILQYLYQHQNVFSTSKVLAEHLSYTDRTIRTYIKKM
                     ASEISERETGFAILSKQGYGYQLRISDEEKYHRFLSENQLVFGVDYSDAENRHKQILN
                     KLIFEEECILFDDLADQLFVSRSTLSHDFKHIREHLASYGLSIESKPYKGVYVVGEER
                     QKRHFIMDYFFSEHFYQNIHHFLSNDKSLALPLSFEELTIVVLDECRDSQLKLSDYII
                     QNLVIHLGLAIQRYQKGFTISPVTIDKEKYAEELAVATKIARRLSHRLEPQNRFPEEE
                     VVYIALHLISKTQLEVEKSSDRLLLRQALFEVLGRYDDEYGYQFSHDFSLIEGLLTHL
                     EVLVERLRHNLHIDNPLLDDIKTTYHDIFDLTQSLMAELSVFHSYSLSESEIAYVALH
                     LMASLERQKEKYKLNVLVICATGYGSAQMLKNRIKNELGQQVSISDVIGYYDLDNQKL
                     QGVDVIMSTIDLSGLVFKVPVITVSVFLTDQEVKEVKQRLADLRPSLQKTQVVERESN
                     LEKYFDQYFSEQRFLVVESAEKEMLLKQMISQLESEGLGNHQEFYKLIEARESLSTLV
                     FDKDIAVPHPLKPISEKHMISVAVIKNGLYWEEGYGQIRLVFLVSPSIYTNEGLSTIT
                     NRLVDLVDLPEVKKDIINSPDFETFKHVFLQRE"
     gene            36613..36927
                     /locus_tag="ACHU66_000315"
     CDS             36613..36927
                     /locus_tag="ACHU66_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFR3388630.1"
                     /translation="MNTEELQMAAFGIILSSGNARSVIHEAFAAMREGDYDKAEELLE
                     AANADMLEAHHSQTGLLQEYASGTEVKVEIIMVHAQDHLMTTMTLREVALEMLALYRK
                     VG"
     gene            36942..37454
                     /locus_tag="ACHU66_000316"
     CDS             36942..37454
                     /locus_tag="ACHU66_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388631.1"
                     /translation="MSKGMASKKLQKTPEQLKQESKRKSQLFNRYMLFRYSLAVFFFA
                     NLYWLLVQLFQPSLYLILPSLMLIAIIIATAEQFKLYSAEESRLTKTEFVLRAQAILQ
                     GLIVVLVILGHISTLFPTFSNHTSAKLFVIGLQLLGIALVVLNLKRLEQVKQNTDKYY
                     LRFQIIRKYL"
     gene            37474..38823
                     /gene="celB"
                     /locus_tag="ACHU66_000317"
     CDS             37474..38823
                     /gene="celB"
                     /locus_tag="ACHU66_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFR3388632.1"
                     /translation="MEEQKGFFGLLDKYLMGPMGKLASYKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVSDIFAASFDKFTPLYMVANTATMGVLSLYFALAIGYEL
                     TKIYAEEEELNLTPLNGALLSLFAFIMTVPQIIFEEGAMSAVSSLEEGAVIVDGWAMG
                     GGVTRFGTTGIFTAIIMAVAAVLLYRMCVKNNWVIKMPDSVPEGVSRGFTALIPSFVV
                     AFFVIFLNGVLVALGTDIFKVIAIPFSFVGNLTNTWIGLVIIYLLTQALWVVGIHGAN
                     IIFAFVSPIALANMSLNAAGERMIVAGEFSNMFVIAGGSGATLGLCIWLATRARSEQL
                     SAIGKAAIVPGIFNINEPLIFGLPIIYNPALAIPFMLAPIASMTVYYFSMKLNLINAV
                     VAQVPWPTPVGIGAFLGTADWRAIVVSIVCAVVAFLVYYPFIRAYDKQLLKEEAANA"
BASE COUNT        11649 a         6965 c         9031 g        11257 t
ORIGIN      
        1 aaaatccgat gtaacaagct tgttttttac agaaagcaga aaatgtggta aaatggagtg
       61 ataaaactat ttgaggtaac gaattatgac tgttaaaatc aatactaaag acggccaaat
      121 cgaattgact gatgacgtga ttgccacagt tgttggcggt gctacgactg agattttcgg
      181 tgttgttggg atggctagta agtctgcgat caaggataat tttcaagcac ttttgagaaa
      241 agaaaattat tctaaaggtg ttgtgattaa gaccttggaa gatggtcgca tcgcagtcga
      301 tgtttatact gtgatgagct acggtgtaaa aatcagtgaa gtatctcgca atatccaaga
      361 acgcgttaag ttcaacttgg aaaatcaact aggtatctct gtagatgccg tcaatgtctt
      421 tatccaaaat atcaaagtcg taggagaata attgtgtcta aaattacaac tagtttattt
      481 caagaaatgg tgcaggcagc atcaactcgt ttgaataaac aagccgaata tgtgaactca
      541 ctcaatgttt tcccagttcc agatggggat actgggacca acatggggat gactattacc
      601 aatggtgcta aagaagtagc agataagtct gcttctactg ttggagaagt tgcgcaaatt
      661 ctttctaagg gcttgttgat gggagctcgt ggaaactcag gtgttattac gtcgcaattg
      721 ttccgcggtt ttgggcaatc tgttaaaggc aaggttgaat tggacggtaa ggatttggcg
      781 caagcctttc aacatggtgt agaagtggct tataaagccg ttatgaagcc agttgaaggt
      841 acaattctta ctgtttcgcg tggggcagcc actgccgctt tgaaaaaggc agaagagact
      901 gatgatgcgg ttgaggtcat gcgtgccact cttgacggtg ctaatcgtgc tcttaagaaa
      961 acgccagaga tgcttccagt attgaaggaa gttggcgttg tcgattcagg tggacaaggt
     1021 cttgtttaca tttatgaagg ctttttgtca gctttgacgg gtgagtatat tgcttcggaa
     1081 gattttgagg cgacacctgc tgttatgtct gaaatgatca atgctgagca ccacaagtca
     1141 gttgcaggtc atgtagcgac tgaagatatt acctttggtt actgtacaga aatcatggta
     1201 gctcttcgcc aggggcctac ttatgtcaag gactttgatt atgaagaatt ccgtaactac
     1261 cttaacgacc ttggggattc cctccttgtg gtcaacgatg atgagatcgt taaagtccat
     1321 gtccatacag aagatccagg tttggtcatg caagcgggtc ttcaatatgg tagcttggtc
     1381 aaggtaaaag tggacaatat gcgtgagcaa catgaagctc aggttgagaa agtagaaagt
     1441 ttccaaaaac cggctgaaca aaaagagtat gctattattg cggtagctgc tggggaaggt
     1501 ttgacagaaa tcttcaaatc acaaggcgtt gactatgtga tttcaggtgg tcagaccatg
     1561 aacccatcaa cagaagattt tgtcaatgct attgaattgg tcaatgcccg caatgtcatc
     1621 ttgttgccaa acaacaaaaa tatcttgatg gctgctcaga cggctgctga ggttgcagaa
     1681 gttgcggtta aggttgtgga aactaagacc ctaccacaag gttttactag cttgcttgct
     1741 tttgatccaa gtcgtgattt ggaaagcaac tttgaagcga tgacggcatc tctcgcagag
     1801 gtgaaaagtg gtagcgtgac aactgcggtc cgcgatacaa ccattgacgg tcttgaaatc
     1861 catgagaatg acaaccttgg tatggttgat ggcaagattg tggtttctaa tccagatatg
     1921 atggagacac ttgtggctac ctttgataaa atgttggatg aagacagcga aatcgtgact
     1981 atctacatcg gtgaagatgg tgatgaggaa ctcgctcaaa ctcttgctga acagctagaa
     2041 gaacaatttg aagatgttga ggtcgaaatc catcaaggaa atcaaccagt ctatccatat
     2101 cttttcagcg tagaataaac aaaatcgcag gctctggctt gcgatttttt ctgtcgactt
     2161 gtctacaatt tccagtagac ctatgacaaa ctactggggc tatggtataa ttaaagacat
     2221 gtatagtcag aatgaaaatg aattgattgc cattggtgag agaattggaa aagcctgtaa
     2281 gccaaatcaa gttctagtat tatcagggga tttgggtgct gggaaaacaa ctctgaccaa
     2341 gggtttggcc aagggtttaa aaattgaaca gatgattaag agtcctactt atacgattgt
     2401 gcgagagtat gaggggacca tgccactcta tcacttagat gtttatcgaa ttggagatga
     2461 ccctgactcg attgatttgg atgattttct ctatggagga ggtctaacgg ttatcgagtg
     2521 gggagaatta ctggatgtca gtctatttga tgactatttg ctcattcgta tagagaaaga
     2581 gggagatggt cgacgattga cagtcgaatc tcatggagct caaagtagcg atttagcgaa
     2641 ggagttccaa aatgtctgaa aaagaagtgt attttagtga agctgaacca gctgatgcag
     2701 cagcttttat tgattttatg aatcaagttg ctagagaaac ggattatctg gtcatggatg
     2761 agactggttt tcgatttagt gttgatgaaa tggagaccat cttcgaagca ggaatcgaaa
     2821 atcctcgtga attgtgcctg ttggctaagg ttggttcgga agtcgtcggc gcaatttctg
     2881 ttaagtcctc taagcagttt cgcattagcc atatcggaaa tatttttatt gcagtaaaaa
     2941 aagcctattg gggtcacggt ctagggacga ttttactgga ggaagtattg cattgggcag
     3001 aggaaatgga tgtattgaag cgtttggaat taacggttca ggttcgcaat caggcggcag
     3061 tacatttata tcaaaaacta ggatttaaca ttgaaggaac ccagatacgt ggggctagaa
     3121 cagatgaagg agaatggctt gatctttatt atatgggcaa gttaattggt gaagtatgac
     3181 aattggaaaa aaaatattct taatgtcttt ggctattatt ggtttaacag taggtgctgg
     3241 cttaatatat ggagccagct tgttaaattt ttctacggat gctatttcga aaacatttaa
     3301 gcagttagat ggagaagaaa aaatcactcc gattgatgcg acagagcccc taactatcct
     3361 actgatgggg gtggatatgg accaagcaac ccgtggagga gattgggagg gtggtcgtag
     3421 tgattcgatg attttggtaa ctgttaatcc caagactaag gaaaccaata tgatgagctt
     3481 gactcgtgat attatggttg agattgcaga agcgaatggg gagtcttcag gaacggttga
     3541 aaaactaaat cattcttata gctacggaca ggcaccgatg gccattgcca cgatcgaaaa
     3601 gatgatggat attaacattg accgctatat cgaaataaat atggatggtc tggttgagct
     3661 tgttgatgcg gttggaggta ttgaggtaaa caatactctt ggcttcccta tatccatttc
     3721 ggaacatgag cctgcctaca cctctattgt ccagccgggg aaacagttgg taaatgggga
     3781 tcaggcgctg gtttatgctc ggatgcgcta tgatgatcct gagggggata ttggtcgtca
     3841 gagacgtcaa cgtgaagtta taacggctat tataaaaaaa ctccttcagt tagacggttt
     3901 tacccagtat aagaagattc tgactgccat ttctaataat atgcgaacca acattgagat
     3961 tagtcctgcg acaattccaa atctcttggg gtacaaggac tctatctcca agttgaattc
     4021 ctatcagttg cgcggtgtgg atcaaatggt tgatgcaatc tattatcaat taccaaccag
     4081 tacacatcta ttggaaatgc aaaatgttct gaaaaaatct atcggcttag aagaaaatac
     4141 cgacttagtc acaaacgtga aggtctacga aggacagatg ggattgccaa gtccaataaa
     4201 tgtttatgat gtatatacaa atctcttgct gttagaagca agtccatggg cagaaagctt
     4261 ggatccagaa atagggagtc catcggctac tacgactgtg acaacagaag agcaggtaac
     4321 cgatttttca acagagccag tactcggtga tgaaaacggg tcacaataat gtagtcattc
     4381 agtttttctt ttattacttt gacgagcgag tataaaagcc tataaaaata gaggttgttt
     4441 gcaacctcta tttttatata tttggaaatt ccctatgtca gggagtgtta ttctcattct
     4501 ggtatttttt gagaatctgt tggatttctt ccaagtttcc tgcaatactt tgctgataga
     4561 ctcgagtctt atattgtaag tcagaagcca tttcctgcaa gggttcttga aaactttgga
     4621 taatggcaag ttgttcctga atattgtcct tgttcagttg aatatctgat agaagttgtt
     4681 tggtttcctg tacttcttta gcgattttat cccgatgcgt gactgcaagg aagctggcgg
     4741 tagcggcgct tacggctaaa atgatattac gaagtttcat attattctcc tagatagagc
     4801 tcttgtgcca gttgagccag ggctgggaag tcagggtcgt tggctttaaa ttgaatgctc
     4861 aatccgtctt gattgttaaa gcgtggtaag agatggatgt gtgtatgaaa gacggtttgc
     4921 ccagcggcct cctcgttatt attaacgatg tttagaccac tagctccaag tttttctttt
     4981 aactgacgag cgatggttgg cacaacagaa aagagtgtgg ctgcagcttc tttatccatg
     5041 tcaagcacgt ttcggtagtg ttttttaggg acaaccaatg tgtgtccttt tgtggcctga
     5101 gtgatgtcta aaaaggctaa tacttggtca tcttcataga cttttgaagc tggtatttct
     5161 ccagagataa ttttgcaaaa aatacaatcc gacataatgg caacctcttt ctgggatttt
     5221 tgttataata attatatcaa attgtaggaa gaaatgtatg ttagaagtaa aaaatgtaac
     5281 tggaggatat gtcaatatcc cagttttgaa agatgtatct tttaccgttg aggatggtca
     5341 attagttggt ctaattggtt taaatggtgc agggaaatca acgactatta aggaaattat
     5401 cggtcttttg accccctatc aaggacagat tgcaattgac ggcctaactt tgctacaagg
     5461 ggctgaaaat tatcggaaga agattgggtt tatccctgaa acaccaagtt tgtatgaaga
     5521 actaaccctc aaagagcact tggaagtggt agcaatggcc tatgatttat cctgggagca
     5581 agcttgggga cgtgttgagc acttgttaaa actgtttcgc ttggatgaga aattggactg
     5641 gtttcctgtc aatttttcaa agggaatgaa gcagaaggtc atgattattt gtgcttttat
     5701 ggtggagccg agtctattga ttgttgatga gccttttctg ggattagacc ctgtggctat
     5761 ctctgattta atcgctcttt tgggggaaga aaaatctaag ggcacttcaa tcctcatgtc
     5821 cactcacgtt cttgactcag ctgagaaaat gtgcgactct tttgtgattt tacatcaggg
     5881 acaggtccgt gcaactggaa atttggagga gctacaaagc gcatttggta tggatggtgc
     5941 aagtctgaat gaaatttatc tggccttgac gcaagaagga gtggctggat gaaagaattg
     6001 tttcaacaac gccgactaga ttttttaaat cgttgttcca agtatctgcg ctatgttctg
     6061 aatgaccatt ttgttttggt tctcatggtt ttattaggct ttttgagttt gcaataccgt
     6121 caattattgt tgtcgttccc tgagaatccg ttgccgattg tttttgtggt tattgcaatc
     6181 agtctactgg tactagtgtc cggaagaatt gcgacttatc tagaagaagc tgatcaaatc
     6241 ttcctattga ccaaggaaaa agacattctg caaggactcg aggttgcagc cagatgcacg
     6301 gtcttgtttt ggtcctctat acaggtggtg gttcagcttg ttttgttacc aatctatttg
     6361 aaactgggtt taccggtgtg gatgattgta gctggtcttc ttactttgtt ggttttaaaa
     6421 tgtctttttg tcaagcgcca attagtggct tatcagtcac agggagtatt ggactggtct
     6481 aaggctattc aagatgaaca aaaaaggaaa cagtctattt tacgtttctt tgccttattt
     6541 acgacggtta aaggaatcag tacgactgtt aaaccgagaa gctatctcaa tggaatcttg
     6601 cgtcttgtca agtcgagaca gacttggttt tatctctact tacgggcttt cttgcgttca
     6661 ggagactact tgggcttgac gatacgtcta ctgttgttgg cgattttgtc cctatttgcc
     6721 attgaagaat cgtggttatc catcggccta gttttagtct ttcattattt gcttgtcttt
     6781 cagttgttgg ggctttacaa gcattatgat tatcaatata taacacaact gtatcctctg
     6841 gagaaagatt ccaagaaaat tggtcttcag aatttgctta gagtgatttt atatagtcta
     6901 ttactggtgg aattgggctt tgctctgcta ttttctaaag agaatacgat ggtagtcgtc
     6961 ttacttctcg tggggatttt cttgcatgaa atatatttgc ctctaaaatt aaaaaaattg
     7021 attgactaaa tagacgaaaa ttagtaaaat agtaaggaat ttttaaagga ggaaaggcat
     7081 gcagtttgat accagtgggc tacaattaca gtctattgca ggaaatagtg gcaaagcgtt
     7141 taaaggccta cggtcagacg gaacgccagt ctttgtgaag tatgagatgc ctccgatagt
     7201 gtctgctctt gcgcgtgagc aaattacacc acccgttttg tcagcaaatc gtgaagtagg
     7261 aatggggcat cgtgtagagc aggagtggtt gaatggtcgt accttggagc gtcaagatat
     7321 gactagtaaa cagattcgtc aaatactggt gagaatgcac tattctcgca ttttgctcaa
     7381 tcaggctttg cagatgaatt atacctatat ggaacctcag gatttggttt atcgttggca
     7441 agaagaggcg ccaactagat tgggacagaa ttcttatctg caatctatct gtcaagattt
     7501 attgaataat ctaccaagat tccgtcaaga ggtggcaaca tttgttcacg gagatttgca
     7561 tcatagtaac tgggtagaaa cgacgtctgg tttggtgtat ttgacagatt gggagaccgc
     7621 ttgtgtaacg gatcggatgt tggatgttgc ctatattttg acgcattata ttcctcgtca
     7681 gtcctgggaa gagtggcttc gggcttatgg ctacaaatat aacagcacag tattaaataa
     7741 ggtttattgg tatggccagc ttggctatct aaaccaaata gctaagcatg tagaaagcta
     7801 caatgttttg gctgcaaata aagaaattta tgctctacgg caattccgtc agagtttcta
     7861 taaggaagta tgagagttag aaatcgtaag ggagcgagtg agcttctagc aaacaatccc
     7921 cagtatgtta tctccaatcc agaggagtgt aaggggaaat gggcagagat ttttggaaac
     7981 aataatccta tccacataga agttggttct ggtaaaggtc gctttgtcac tggtatggct
     8041 gcgcaaaatc cagatattaa ctatattggt attgatattc agatgaccgt gttaagctat
     8101 gccttggatc gtgtgcttga agctggctta ccaaatataa aattattgca ggtggatggt
     8161 tcgagtttga ccaactattt tgcaccggca gaaatagatc agctttatct gaatttttca
     8221 gatccatggc ctaagaaacg tcatgagaaa cgccgtttga cctacaagtc attcttggat
     8281 acctacaagg aaatcctacc tgagaaggga gaaatccact tcaaaacaga taatcgtggt
     8341 ctgtttgagt atagcctggc tagtttttct caatatggca tggtcttgaa gcaagtttgg
     8401 ctagatttgc atgctgatgg tttggaaggc aacgttatga cagagtatga ggagaaattc
     8461 tcaaacaagg gtcaagttat ctatcgtgtg gaagcacagt ttcaataatt cccttagtgg
     8521 aaaatgagga gaggtctaac agagaccttt ttcctttaca aatagggata gatatgatag
     8581 aataatggaa gtgaataatg agaaaagggg ttggcgtcag tcaacccttt ccctgtacac
     8641 ggatttctgt atatagaatg aatgatagta caagggagtt tactgtcaaa ggaaatagag
     8701 agtcctggat agtgcgaact atttgagtaa tgtatttcta gcgactgtaa aaataggagg
     8761 tgtttatgtc atcaattatt gaattggtca cagcagcaat tacccccgct attcaaactc
     8821 cttacgagtt ggtagatgtg gaatatggta aaatgggcgg tgactatgtc ctatcgattt
     8881 ttgtcgataa ggagggtgga atttcccttc aagatacggc agatttatcc gaaaagatca
     8941 gtccgatttt ggacactatc aaaccagacc catttccaga ccaatacatg ctggaggtaa
     9001 caagtccagg tttggaacgt cctttaaaaa cggcagatgc tgttgagaag gcggttggaa
     9061 agtacatcca tgtcaagctc tatcaggcaa ttgataaaat caaggttttt gaaggaacgt
     9121 tgctctcctt tgatggaaca gacctgatta tggagtatat ggataaaact cgtaagaagg
     9181 aagtgactat tccttaccag acggtggcta aggcccgttt ggctgttaag ttataatgct
     9241 ctttcatgag tattttaaaa gatattagac tttaagaaag gaccttttga gcgcaaaagg
     9301 aaaacaacat gagtaaagaa atgctagaag ccttccgtat tttagaggaa gacatgggta
     9361 ttaacaaagc ggatatcatt gatgcagtta cagagtcgct tcgttcagct tataaacgcc
     9421 gttatggtca atcagaatca gcagtgattg aatttgacga gaaaaaaggc gatttccatg
     9481 tatttactgt tcgtgaagta gtagatgaag tatttgatag ccgactagaa attagtctaa
     9541 aagatgcctt ggctatttca tcagcttatg aaatgggtga caaaattaaa ttccaagaag
     9601 acccagcgga atttggacgt gtagctgctc aatcagctaa acagaccatc atggaaaaga
     9661 tgcgcaaaca aaagcgtgcc attaccttca atacctacaa gcaacatgaa aatgagatta
     9721 tgtcaggtac ggtagagcgt tttgataatc gctttatcta tgtcaacctt ggtacgattg
     9781 aagcacaatt gtcaaaacaa gaccaaattc ctggtgaggt tttccaatct catgatcgca
     9841 tcgaagtcta tgtctacaag gtagaagaca atggtcgtgg tgttaacgta tttgtcagcc
     9901 gtagccatcc agaaatgatc aaacgtctta tggaacagga aattccagaa gtatacgatg
     9961 gaacagttga aatcatgagt gttgcccgtg aagctggaga tcgtacgaaa gtggctgttc
    10021 gtagccataa tccaaacgta gatgccatcg gtacgatcgt tggtcgtggt ggttcaaata
    10081 tcaagaaaat caccagcaag ttccacccag ctcgttatga tgctaagaat gaccgtatga
    10141 ttcctactga ggaaaatatt gacgttatcg agtgggtagc agatgaagcg gaatttatct
    10201 acaatgctct cgctcctgcc gaagttgacc aagtattgtt tgacacggaa gatggaaaac
    10261 atgctacagt cgttgtacca gatgataaat tgtcattggc aattggtcgt cgcggtcaaa
    10321 acgttcgttt ggcagcacat ttgacaggat tccgtatcga catcaagtca gcatcggagt
    10381 atgaagcttt tgaagcaact caatatgcaa cagacgaagt tgtagaagaa gtcgcagaag
    10441 aacaagaata aggcaaaagg agcaaccatg gcaaaagcta gaaaaatacc cttgcgcaag
    10501 tctgtggtgt ccggtgaaat catcgacaaa cgggatttgc tccgtattgt aaaaaataaa
    10561 gaaggtcaag tatttatcga tccaacaggc aaggcaaatg gtcgtggagc ttatatcaag
    10621 cttgacaatg atgaggcaag ccaagctaag aaacgtcgtg tcttcgatcg ttcctttagt
    10681 atggaagtgg ccgaagaatt ttacgatgaa ttaattgcct atgttgatca taagatcaag
    10741 aggagagaac ttggactcga ataagagaca gaaaatctta aatttattag gtttggccca
    10801 gcgagctggt cgcctggttt cgggtgagga cctggttgtt gaagctattc aaaaagggca
    10861 ggcaaagttg gtgtttttag ccgaagatgc tgctggaaat ttaagtaaaa aagtaacaga
    10921 taaaagtcat acctatcaag tagaagttgt aacagtgttt tcaacactgg aattgagtgc
    10981 agctgtcggc aaggctagaa aagttcttgc agtgacagat gctggattta caaagaagat
    11041 gaggtctatt atggaataga acaggaggac aaggcattgt ctaagaagag attgaatgaa
    11101 atagcccgtg aattaggcgt aagcagtaag gaagttgttg ctaaggctca agaattgggt
    11161 tttgaagtca aaagtcatgc ttcaagcgta gatgaagcaa gtgctaaacg ccttgcagaa
    11221 agctttggag gacaaaaatc agaggcaact aaagttgcgg caaaagttag caagcctgag
    11281 aaggttgatg aaactccaaa agtggaaaca gctaaggtag aaaaagctaa ggaaacacag
    11341 ccagtagtaa aagaagaagt tgcggcaagc gcagtacaat cagttccaca tcgtccacag
    11401 agtcgaaact ttaaggcaga gcgtgaagca cgggccaaag agcaggctgc taagcgagct
    11461 cagggtcaag gaaaaggtgg tcaagctaaa tctggtcagg accgtcgtga caaccgtcaa
    11521 caaggtcagg gacgttcaaa caatgagcga aatgatcgcc gcgacaatcg tcgtgaccag
    11581 cgtccagaag agcggaaaga caaccgattt ggtgaacgcc gcgataatcg tgccaaccgt
    11641 cgtcaggaca atcgttcagg tcggtcagct cgctttgaac aaagagaggc tgcaaaacca
    11701 gcaggaccta aaattgactt caaagcgcgt gctgctgctt tgaaagcaga acaaaatgcg
    11761 gagtatgctc gtaccagtga agaacgtttt cgtcaggctc aggaagctaa aaagcaacct
    11821 aagaaggcaa aagaaattaa gtttgaagaa ccggttgtgg aaagcaaacc atttgtgaag
    11881 cctgcacctg ttgcatcagt accagagcaa gttgcagaaa ctactgtaga tacccgtcgt
    11941 aagaaacaag ctcgaccaga taaaaaacgt tactttaata gtgacgaaga agatggtcca
    12001 cggaaacaac aaagaaatcg aaatagtcaa aatcaagtga gaaatcaaag aacaagtaac
    12061 tggaataaca acaagaaaaa taaaaaaggc aaggctaacc aacctgccaa acctgtcaca
    12121 gaacgcaagt tccacgaatt gccaactgaa tttgaataca ctgctggcat gaccgtcgca
    12181 gaaattgcaa aacgaatcaa acgtgaacct gctgaaattg tgaaaaaact cttccttatg
    12241 ggagttatgg cgactcaaaa ccaatctttg gatggagata ctatcgaact cctcatggtt
    12301 gattatggta ttgaagcgaa agaaaaggta gaggtcgata acgcagatat tgagcgtttc
    12361 ttcgtagaag agggctacct caatgaagaa gagatgacag aacgcccacc tgttgtgact
    12421 atcatgggac acgttgacca tggtaaaaca accttgttgg ataccctacg taattctcgt
    12481 gttgctactg gggaagctgg aggtatcaca cagcatatcg gtgcctacca aattgaggaa
    12541 gctggtaaga aaattacctt cttggatact ccaggacacg cggcctttac ttctatgcgt
    12601 gcacgtggtg catctgttac cgacttgacc atcctagtcg ttgctgcaga tgatggtgtc
    12661 atgccacaaa cgattgaggc tatcaaccac tcaaaagcag ctaatgtacc aatcattgta
    12721 gctatcaaca agattgacaa gccaggtgca aacccagagc gtgtcatcgg tgaattggct
    12781 gagcacggtg ttatctcaac cgcttggggt ggagaatctg aatttgtaga aatctcagct
    12841 aagtttaacc aaaacattga tgaattgttg gaaactgttc ttttggttgc tgaaatccaa
    12901 gaactgaagg cagacccaac tgttcgtgcg attggtacag ttatcgaagc gcatttggat
    12961 aaagggaaag gtgcggttgc aaccctcctc gttcaacaag ggacacttaa tgttcaagac
    13021 ccaatcgttg tcggaaatac cttcggacgt gttcgtgcca tgacaaacga ccttggtcgt
    13081 cgtgttaaaa ctgctggtcc atctacaccg atatctatta ctggtttgaa cgaagcgcct
    13141 atggcaggcg atcattttgc agtttacgag gatgagaaat cagcccgtgc agcaggtgaa
    13201 gaacgtgcta aacgtgctct tcttaaacag cgtcaagcaa cgcaacgagt tagtcttgaa
    13261 aacctctttg atacccttaa agcaggtgag gttaagtcag ttaatgtgat tatcaaggcc
    13321 gacgtacaag gttcagttga agctcttgca tcatcgcttc aaaagattga agtagaggga
    13381 gtacgtgtca atatcgtcca ctctgcagtt ggtgctatca atgaatcaga tattactctt
    13441 gcggaagctt caaatgctct tgtgattggt ttcaacgttc gtccaacagc tgaagcgcgt
    13501 agccaagctg aagcagatga tgttgaagta cgtcttcaca gcatcattta caaggttatc
    13561 gaagagatgg aagatgccat gaaaggtatg cttgatccag aatatgaaga aaaaatcatt
    13621 ggtgaggcaa tcattcgtga aaccttcaag gtatccaaag ttggaacgat cggtggtttc
    13681 atggtcgtac gtggtaaggt tacccgtgat tcaagtgttc gagttatccg tgatggtgtt
    13741 gtagtatttg atggtaaact agttagtttg aaacgctata aagatgacgt gaaagaagtt
    13801 ggtaatgccc aagagggtgg tttgatgatt gaaaactaca acgacttgaa agttgatgat
    13861 accattgaag cttacatcat ggaagaaatc aaaaaataat attttctcat tgtcaaaatc
    13921 atagacaagt gattttgtcg aggaagtgac aatttaatag attttagtag attttaaagc
    13981 aatagttttt aatctacttg gattattcaa gctggggaaa ctcagctttt ctatcatcta
    14041 agaaaggaaa gaatatggcg aatcattttc gtacagaccg tgtcgggatg gaaatcaagc
    14101 gcgaagtcaa tgagattttg caaaagaaag ttcgtgatcc acgtgtgcag ggagtgacca
    14161 ttacagatgt tcagatggtt ggcgatttgt caatggccaa ggtttactac acaatcatga
    14221 gtaatttggc atccgataat caaaaagcac aaacgggtct tgaaaaagcg acgggaacca
    14281 ttaagagaga acttggtcgt aagttaactc tttataaaat tccggatttg gtctttgaaa
    14341 aagaccaatc aattgagtac ggcaataaaa ttgaccaaat gttgcgtgct cttgatcaga
    14401 aagattaaca acaaaagtcg gatggaaaca ttcggctttt tctggctcgg atgctttgtc
    14461 catatatggt ggcgtttaca agccctcgga aagatgttat actaatactc aatgaaaatc
    14521 aaaagtagac taggaaacga agtcgaagat agaactctgt cactatctta tttcaaggta
    14581 acaggctgaa aacctccact ggagcttttc actcatcaag gcaagttgac aacggataat
    14641 tttgattttc gaagagtata agaccaaaga tagagcacag gaggaagtta tgaaaacatt
    14701 tattagtaaa ctgatgttag tgtccacggt tgtactatta gctgcttgtc agtctaatca
    14761 ggtaaaaggt ggtgagagtt ctagttctag tgcttcaagc caagtttcag atggttctag
    14821 tttgcagcaa gcatcaacaa tagcatctag ctctgagacg caagcatcct cagtgaccac
    14881 tcctgagcta agtgaagtat accaatcagt tattgatcgt tatcaagcga atatggggca
    14941 gtctgctgag gcaattaatc aggatgaggt cagtagctat ctgagcttat taactagcca
    15001 ggggcaagaa tacagtggaa tattttatag tttgtatgat gtcaaccatg atggtacaga
    15061 ggagctgctt ctagctttgg acaagtcggg agaatatgtc ttaattgatc tctatactca
    15121 gcttgccgat gagagtctac gtttggtgga taatttccgc aatcttggat ttgaaattgg
    15181 accgaacgcc ctacttcgtc ctttacagga tggaacatac ttatttgaag gagatggtat
    15241 ttttcgcatt tatcaataca atgcgatgat tccaggttta aaacggattt ctgaatcaga
    15301 taccaatcct gaaacttctc ccttgcttga gttgaaaagc ttgcattggg ttaagctata
    15361 gattttatca atgattattc ctaggctggg tttcccagcc ttttgtagtt tctaagaact
    15421 ttaagttttc ttttggtaat ctttaatctt tcttttagga aggaaaggta aactaggagc
    15481 atatcaggaa gggatacgac ttcctaaaga aagaaaagga atctacatta tgaaaacaaa
    15541 tcttaaaaaa ttactttact ctggtgttac tcttatgagc cttggtgtct tagcagcatg
    15601 ttcatctact tcaagttcaa cgactacttc aagctcagca gcaactagtc agacaagtgt
    15661 agcgacaact tcaaataata gcacttcatc agattcaagc tcgtcaacta ttgactggtc
    15721 agcccttcca acgacagaag tgacgctttc aaatgatggc ttgaaaatta ctgagggtgg
    15781 aacctatatt ttgactggtt caacaacagc tggtgtaacg gttgaaacag atgccaatgt
    15841 tcgcatcatt ctagcaggtg ctgagatttc aagttcagat acagcagcaa tcaatgttat
    15901 taatgctgac aatgttgaat tggaaatcaa agatggtacg acgaatacag tgaaagatac
    15961 cagcaatcat acagacacca atattgaagg ggctatccat gttgaagctg atttgaccat
    16021 tacaggaaat ggtagcctga ccgttgaagg taacttccaa gacggcatcg tatcgacgga
    16081 cgaccttgtc gtgaattcag gaaacattac cgtcactgca gtggatgatg gtatccgtgg
    16141 taaggattcg ttgacaatca atggtggtga aattactgta acagcaggtg atgatggtat
    16201 taaagcggag acggctttga caattgacgg aggcaccatt acagtatcag aatcagcaga
    16261 agctcttgaa ggaacgaata taaccatcaa tggtggtaca ttagatgttt acggttcaga
    16321 tgatgctatt aatgcggcaa gtacaacgtc atcagaaatc ttcatcaagg taactggcgg
    16381 tgacttgaaa gtagctgtag gtagtggaga tacggatgcc tttgatgcta atggagacat
    16441 ttatatatct ggtggtacaa tcgatgtaac agctcaatct gcttttgact tcgacggaac
    16501 tgctgaattg acaggaggta ctgtgacagt taatggtgag cagattactc aaatcacagc
    16561 aacaggacca ggtgctggtg gccacggtgg ctggtaatca ataataatac aaaaaggtct
    16621 gagttgacga actcagacct ttcgtttgct ttccatattg ggaagattga ttttgtattt
    16681 aattaccaat aatcgaatga ttaaagaaat ggtaaaaata gtgatgaagg ttggaatttg
    16741 tggtaatttc caatttacaa cgcaaagatg atagaggact cctgagaaaa atgcaaggac
    16801 agcatagaca tcttctttaa gaacaatggg tgtttcattg acgagtaaat cacgggcgat
    16861 tccgcctccg acacctgtca aagctgccag aataccactg gttagagcgt ttagctggag
    16921 agagatgcca gtactggctc caatgagggc gaagatggct agaccgattg catcaaagat
    16981 aaggtttgtc tgggaaagga aatgaatcat cttcttatca agtggtttgt cctgctttag
    17041 attgataaca atcaagtaca tgctaatcga tactccgaga gataaataga tggctgtagg
    17101 atcagttaag gcggcaggaa tgcgattggc catcgtatct cgaataattc ccccaccaac
    17161 ggcggtaatg atgccgagca agctaattcc aaaaatatct aactttttct taaatccttt
    17221 tacggccccc gaaacagcaa atgcgatagt gcctatgtag ttacaaatca taagaaataa
    17281 atcgaattcc ataagaacct ccgtacctat attaacagaa agaaagatgg cgtgcaagct
    17341 tattttaaag tttgtgaaaa aagtttcaaa caagtaaagt gaatctttta atgatttgtg
    17401 aaaaaacgca ctaaatcaag gttttcacaa aaatttctgt aaaaagaaaa cggttcaatt
    17461 acttgctatt tgtgaaatat tggtctatca tagtacagaa agagaaaaac ctttggagga
    17521 aattatggta tctatcacaa aagaacaaca tttggacatg ttcttgaaaa tgcaacaaat
    17581 ccgtgatgtt gatatgaaat taaacaaatt agtgcgtcgc ggtttcgtac aaggtatgac
    17641 tcacttctca gtcggtgaag aagctgcggc tgttggacct attgctggat tgacagatga
    17701 ggacatcatc ttctcacacc accgtggtca cggtcacgtt attgcaaaag gtattgacat
    17761 caacggtatg atggctgagc ttgctggtaa ggcaactggt acatcaaaag gccgtggtgg
    17821 ttctatgcac ttggccaatg ttgaaaaagg aaactttggt tcaaacggta tcgtaggtgg
    17881 tggttatgcc cttgcggtgg gtgcggccct tacgcaacaa tacctcggta cagataacat
    17941 tgttattgcc ttctcaggtg attcggctac taacgaaggt tcattccacg aatcaatgaa
    18001 cttggcagct gtttggaact tgccagttat cttcttcatt acaaacaacc gttacggtat
    18061 ttcaactgac atttcttatt ctactaagat tcctcacctc taccaacgtg cagcagcata
    18121 cggtattcca ggtcattatg ttgaggatgg taacgacgtt attgctgttt atgaaaaaat
    18181 gcaagaagtc atcgagtacg ttcgtgcagg taacggtcca gccatggtcg aagttgaatc
    18241 gtaccgctgg tttggtcact caactgccga tgcaggtgtt taccgtacta aagaagaagt
    18301 caatgagtgg aaagcaaaag acccacttaa gaaataccgc aaatacttga cagagaacaa
    18361 gattgcgaca gacgaagaat tggatgccat cgaagcacaa gttgcagaac aagtagaagc
    18421 atctgtgaaa ttcgcacaag aaagcccaga tccagacatc tcagtagctt atgaggatgt
    18481 gtttgtagac tagggtgtga ggacgttaaa gaggcacaga aaaattaggg agtcagctag
    18541 tgatgtggac atcactaatg gctcatctat ttttccaagc ctctagtcag aacctaacta
    18601 caaacgtaag aagtttgact aagctctaaa tagcaagtca aactagggtg gactacctac
    18661 tcaagttcga gtacaattct aaggtgtctt aagctgggac aataaacaat tacatcgtag
    18721 taattgtttg gttttcctta gttcagtata gataaattga tataaattag gagtaaaaaa
    18781 tggctgaaac aaaagtaatg gccttgcgtg aagcgattaa cttggctcaa agcgaagaaa
    18841 tgcgtaagga tgaaaaagta ttcttgatgg gtgaagacgt cggtatctac ggcggtgact
    18901 ttggtacatc tgttggtatg ttggacgagt ttggtccaaa acgcgttcgt gacacgccta
    18961 tctctgaggc tgcaatcgct ggttctgcgg ttggtgcggc tcaaacaggt cttcgtccaa
    19021 tcgttgactt gactttcatg gacttcatca caattgccct tgatgcaatt gttaaccagg
    19081 ctgccaaaac caactacatg tttggtggtg gcttgaagac acctgtaacc ttccgtgtgg
    19141 catctggttc aggtatcggt tcagcggcac agcactcaca atctcttgaa gcttggttga
    19201 ctcacattcc aggtatcaag gtggttgcac caggtacagc taacgatgca aagggtcttt
    19261 tgaaatcatc tatccttgat aacaaccctg ttatcttctt ggagccaaaa gctctttatg
    19321 gtaaaaaaga agaagttaac ttggatccag atttctacat tccacttggt aagggtgaaa
    19381 tcaaacgtga aggtacagat gtaaccatca tttcatacgg tcgtatgttg gaacgtgcct
    19441 tgaaagctgc tgaagaagtg gctgcagaag gaatcagcgt agaagtggtt gacccacgta
    19501 cccttatccc attggataaa gaattgatca tcgaatctgt gaagaaaact ggtaaggtta
    19561 tcttggttaa cgatgcttac aaaacaggtg gtttcatcgg tgaaatcgca tcaatcatca
    19621 cagaaagcga agcctttgac tacttggatg caccaatcat ccgtatcgct tcagacgatg
    19681 taccagttcc ttacgcaaac attcttgaaa acgcagtatt gccaaacgta gagaaaatca
    19741 aagcggcaat ctataagcaa gtaaacaagg gttaatctta tcgtcatccg cttatctttt
    19801 ctggctgtgt tgatttggat tgcctaacgt cagttatgcc tgcactaaat cgctttatca
    19861 gaaaagcaat cggaaagacg ataaccctaa tatgctagaa ataaaatgtt ctagcatgac
    19921 taagaatgct tggaaaagaa ttgagccgct tcgattatcg aagtctggtt tggttcttga
    19981 caaggtaaga aactgaaaga aaggaattga acccgtccgg aaaactcgga aaaaagataa
    20041 atcgttacga aaatcaggat tttcgacgat ttcctagatt ccctcgtttt ctgttcggac
    20101 ttggtatctt aataatggcc attgaaatca ttatgcctaa gctcggtgtg gacatgcaag
    20161 aaggtgaaat catcgagtgg aaaaaacaag agggcgattt cgtcaacgaa ggcgacgtca
    20221 tcttggaaat gatgtctgac aagaccagca tggagctgga agcagaagag tcaggtgtcc
    20281 tattgaaaat tgttcacgga aacggtgcta ccgttcctgt aacagaagtt attgcctaca
    20341 tcggtgcaga aggtgaaacc gtcgaagcag gcgcttcatc tgctcctgct gttgaaccag
    20401 cagcggctat tgaagaagta cctgcgggtc gtacaccagt aatcgttgct cctgcaactg
    20461 ctgccaaacc acaaggtagt ggcaaggtgc gtgcaacgcc agctgcgcgt aaattggcgc
    20521 gtgagcttgg aatcgacttg ggtcttgttc caggaacagg tgctaacggt cgtgtccaca
    20581 aggttgacgt cgaagacttc aagggagcag ctccaaaagc aactccgctt gcagcccgta
    20641 ttgcagcaga ccaaggtgtt gacttgtcaa cccttacagg ttcaggtgtc aacggtaaga
    20701 ttgtcaagaa cgacgttctt gcagtacttg ctcctgcagc tgtagagact gctgctccag
    20761 caccgaaagc agaagaaaaa ccagctaaag aattgccaga aggcgttgaa atcatcaaga
    20821 tgagcccaat gcgtaaggcg atttcaaaag gtatggtcaa ctcttacttg actgctccga
    20881 catttacact taactacgat attgacatga ccaacctcat ggcacttcgt aagcaagtcc
    20941 ttgagccaat catgaacaag actggtttga aagtaacctt tacagacttg atcggtcttg
    21001 cggttgttcg tactttgatg aaagaagaac accgctacat gaacgcatct ttgattaacg
    21061 atgctcaaga aatcgaatta cacaagttcg tcaaccttgg tatcgcagtg ggtctagatg
    21121 atggcttggt ggttccagtt gttcacggcg cagataagat gagcttgtct gactttgtgg
    21181 tggcttcgaa agatgtcatc aagaaggctc aatctggtaa gttgaagggg gctgaaatgt
    21241 caggttcgac cttctctatc accaacttgg gtatgtttgg tactaaaacc tttaacccaa
    21301 tcatcaacca accaaactca gcaatccttg gtgtagcatc aacagttcaa acaccagttg
    21361 ttattgacgg cgaaatcaaa atccgtccaa tcatggcact ctgcttgacc atcgaccacc
    21421 gtatcattga tggtatgaat ggtgctaagt tcatggttga cttgaagaac ttgctggaaa
    21481 acccattgga attgttgatc tgagataaga tgcaaaaggc gttaagaaaa ccgtatgaaa
    21541 ataggaaatc aacgatgtgc cttgaggcac gaggcgattt atctttttca ataggttttt
    21601 agcctgtgct caatttggta aagagaaaaa ataaagaaag ggaataagag cgagagagtt
    21661 ttagcaattg aacccgagcg gaaagctcgg aaaaaagata aatcgtttag agaatcagga
    21721 ttttctacga tttcctattt ttcagtcgct ttctcttcgc tcttggtatc ttaactatgg
    21781 caattgaaat tattatgcct aaacttggtg tagatatgca agaaggcgaa atcatcgagt
    21841 ggaaaaaaca agagggtgat tttgtcaacg aaggtgatgt tatcttggag atgatgtcag
    21901 acaagacaag tatggaattg gaagcagaag aatcaggtgt actcttgaaa atcgttcatg
    21961 gtaacggtac aactgttcct gtaacagaag tcattgctta ccttggtgca gaaggcgaat
    22021 cagttgaagt tggtagcgca cctgctccag ctgaggttgc acaagcaact gccgacttga
    22081 aagcagctgg tttggaagtg cctgcagctc ctgcagcagc tccacaagct cctaaggctg
    22141 aattggcagc tgacgagtac gacatggttg ttgtcggtgg tggtcctgct ggttactacg
    22201 cagctattcg tggtgctcaa ttgggcggta aaatcgctat cgttgagaaa tcagaatttg
    22261 gtggtacctg cttgaacaag ggttgtatcc caactaagac ctaccttaaa aacgctgaaa
    22321 ttcttgacgg cttgaagatt gctgctgagc gtggtatcaa ccttgcttca acaaactaca
    22381 ccgttgatat ggacaagaca gttgacttca agaacaaggt tgtgaagaca ttgactggtg
    22441 gcgttcaagg tctcttgaaa gctaacaagg taacgatctt caacggtctt ggtcaagtaa
    22501 accctgacaa gactgttgtc atcggcgaca aggtcatcaa aggtcgcagc atcattcttg
    22561 caactggctc taaggtttct cgtatcaaca tcccaggtat tgattctaaa ttggtgctaa
    22621 cttctgatga tatccttgac ttgcgtgaaa ttcctaagtc actcactgtt atgggtggtg
    22681 gcgtagtcgg tgtggaactt ggtttggttt acgcgtctta cggtactgaa gtaacagttg
    22741 ttgaaatggc tgaccgtatc attccaggta tggaccgaga agtgtctgtt gaattgcaaa
    22801 aagtcctttc taagaaaggt atgaaattct tgacatcagt tggagtatct gaaattgttg
    22861 aagccaacaa tcaattgaca attaaattga acgatggttc agaaattatc tctgaaaaag
    22921 ctcttctttc aattggacgt gtacctcaat tagctggtct tgaaaatctt caccttgagt
    22981 tggatcgcgg tcgtatcaag gttaatgctt accaagaaac ttctatccca ggaatttatg
    23041 cacctggtga tgtcaacggt actaagatgt tggcacacgc tgcttaccgt atgggtgaag
    23101 tggcagctga aaatgctatt cacggtaacc accacaaggc taaattggac ttcacaccag
    23161 cagcggttta cacacaccca gaaatcgcta tggttggttt gactgaagat caggctatcg
    23221 agaaatatgg taaagaaaac atccttatcg gtcgcaacag ctttactggt aacggtcgtg
    23281 ctattgcttc taacgaagca catggtttcg taaaagtaat tgcggacaag aaataccatg
    23341 aaatccttgg tgttcatatc atcggtccag ttgcagctga aatgatcaat gaagcagcaa
    23401 ctatcatgga atctgagttg acagttgacg acgtggcagc atccatccac ggtcacccaa
    23461 ccttctcaga ggttatgtat gaagccttcc tggatgtgct tggtgttgca atccacaacc
    23521 caccaaaacg taaataatag cattcagttt atcttttatt acttcgttaa ctcgatttgc
    23581 cgtactctag tacagcctgc atctcgttgc ctagtactaa aagcaaactg aaatgctaac
    23641 atctaaaaga aagtccatat ggctttcttt tttgtatgtt catagtatag tattagtcta
    23701 tcgcagttta ggactgttat ttgatagaaa aacagactat acttgtagta gaaaaaggca
    23761 aggagaaaac agatgcgcaa gattattttt ttagatgtgg acgggacctt ggttgactat
    23821 cataatcgga ttccagaatc tgccattcgt gctattcgac aggccagaga aaatggacac
    23881 ctggtctatg tctgtacagg tcgaagccga gcggaaatgc aaccagaact atgggagatt
    23941 ggcctggacg gaatgattgg tggcaacggc tcttatgtcg agcatcaagg tcaggtcatc
    24001 atgcaccagc tcctatcaga agaggatagc agagcgattg tggactggct ccatgaacgc
    24061 ggcttggaat tttatctgga aagcaacaac gggctctttg ccagtgaaaa tttccgtgaa
    24121 cgtgcccgtg aaaccctgcg aatttattcc atgaacaagg ggaaaactgc agaagaggtg
    24181 gccactcagg aagtagatga cgttatacac ggcatgattt ttgatagtca actctaccgc
    24241 aatgatctca acaaagtcag ttttgtacta gactcctatc aggatcattt ggactctaaa
    24301 gaggcctttc cgcaactggt cgcaaacacc tggggaggtc gcggagagtc tgccctcttt
    24361 ggcgatttgg gtgtaaaaga tattgacaag gcccatgcta tttctgtttt gctggatcac
    24421 ttaggagcta gtcaggcaga cactatcgcc tttggtgacg ctaaaattga tatttccatg
    24481 ctggactatt gtgcggttgg ggtcgctatg ggcaatggcg gagcggaaat cttggctatg
    24541 gcagatatga taacggacga tgtagaagaa gacggcctct acaatgcctt tgaaagattg
    24601 ggactacttg acaagtagct ctcagaatcc cttgcaaaac cgcttacaat ctgataagat
    24661 aagtgtagga ttgttactga taagcaggca aaacctaagt agatagtgaa gggtctggca
    24721 acagtcgtat ttgctatgtg cttgggttct tttttatcag tcgaaaaaag aggcggtaaa
    24781 gtagatgcag attgaaaaag tttataacaa caatgtcatt caagcaatag atcagcaggg
    24841 ccgtgagttg attgtcatgg ggaaaggatt aggttttcag aaaaaagcag gggaagaatt
    24901 agacaccagc aaaatcgaga aaacctttgt gctacaaaat gactaccaac aatcagattt
    24961 atcgagtctg tacctgcaaa tggagagcgg tgaagtcgaa gtagtcaatg ccattatcaa
    25021 tagggcagaa gaaactttag aagttcagtt tgatttgtct ttgtatctag ccttagcaga
    25081 ccatttacat tttgtatttc agcgatgccg tgaaggtatc tttattgaga acccgctgtc
    25141 ttgggaagta cgtaagtttt atccgaagga ataccaaatt ggtttagaag ccttgcagtt
    25201 agtgcaggag cgtttaggct tggaactagg aaagggagag gcatcgtcta tcgctctcca
    25261 tctggtaaat gctcagaaga atggtgcttt tggcaaggaa acccaaacca ttagtaaaat
    25321 cgtgactcag attttagaca ttgtccgcct tcattttggt agtgtgacct acgaagaaga
    25381 taccagctat catcgatttg taacacatat tcagtatttt gctcaacgtg tggccaatgg
    25441 tgtggtcgaa ggggtcaatg atgcattttt gtatgaacag gtgatggcta actatcctga
    25501 ttcatttgct tgtacgacta agattcgtca gcatattgcg gaagtttacc attttgaaat
    25561 gtccaaagat gaacaagtct atttgaccat ccatatccag cgcttaaaaa cgagttagaa
    25621 aaaaatcaaa aaaactttag aaaacgcttg caaaaaaatt tgtaatgtgt tattattatt
    25681 tcaacaaaag gattgttact ggttagcagg caaaacctaa atatatacaa gatactcttt
    25741 ttgacgtagt tcaaggggca gtgtttggta tatgtttagg tttttctgtt tttcccagaa
    25801 aagaaaggag ccgaaaaatg gctaaggatt atacagattt agctacagat attgtagctc
    25861 atgttggtgg aaaagaaaac atcagcagtt tgaaacactg cgtgactcgt cttcgttttg
    25921 gtctcaagga tgagagcaag gctgatacag actacttgaa ggcgcgtgat ggcgttgtga
    25981 cagtagttca agcaggtggt caataccaag tggttatcgg caaccatgta cctgatgttt
    26041 atgcagcggt acaaaaagtt gctggtatct caggggatgg cgccttagat attgatgaag
    26101 gtgacggacc gaagggaaat ctgtttgacc gatttgttga cctagtttca ggtgttttcc
    26161 agccttcttt aggagtgctt gcagctgcag ggattatcaa aggattggta tccatcctag
    26221 ccgctacaat gggtttgaac aatgataata gcgccctata tgctatttta aacacagctg
    26281 gagatggatt cttccaattc ttgccaatcg tcttagcttt caatgctgca cgaaaattca
    26341 gaatgaatga atttactggt atggcaattg ctatggcatt ggtttaccca actctgccag
    26401 ggacatttgc gactctaaaa gaagcgggtc taaataatgt ttttggcata ccatttgtac
    26461 ttccagcagc aggtagctac ttgcaaactg tgatgcctgt cctacttgct gtctggattg
    26521 cttctcatat tgagaaattc atgaagaaaa ttacgcctga cgtagttaag gtcttcattg
    26581 taccgtttgt gacaattcta atttcagttc cattgacatt cttggttgta ggtcctgtcg
    26641 cgaactacgc ttcagatctt ttgtctgcag gctttactgc ggttatgggc tttagtccac
    26701 tactttacgg tattctactt ggtgctttat ggcaagtaat ggttatgttc ggacttcact
    26761 gggccttggt accacttgca atacttcagt tttctcaaaa cggatggtca aatattttgt
    26821 tagctgcagc gcttccaaac tttactcaaa ctggtgttct tttggcaatt atgatgaaaa
    26881 ccaaagaatc aaaagtgaag tcagttgcga tgcctgcatt catttcttca atctttggtg
    26941 tcactgagcc tgctatctat ggtgttactc ttcctatgaa aacaccattc tacatttctt
    27001 gtgcagtgtc agcagtaatt ggtgcaggtc tctcattctt tgatttgaaa aactatgcta
    27061 tgggtgctct cggtatcttt atgtatccag gttatgttag tcctgaacta ggtgtgactc
    27121 caatgtggat tatgatgggc ttctctttgg cggcaattgt tttatcattc gctatccaaa
    27181 tggctcttcc agttccttac ttatatggag aaccaactga aaaaaagtcc gttgaagaaa
    27241 caaaggagtc tgttccagaa ttaaaagaaa ttaaacaaga gatcattgct agtccactaa
    27301 tcggtaaagt agttaaatta gaagatgttc ctgatgcagt ctttgcttca ggagctatgg
    27361 ggaaaggaat tgctatcgat cccctagatg gaatccttgt atcaccagcg aaggctgaag
    27421 tcactcttct attccctacc aagcacgcca ttggtcttcg gacggaaaat ggggcagagc
    27481 tactcatcca tatcggtatg gacacagtct ctctagctgg taaaggcttt gaatccttcg
    27541 tccaagtagg tgaccaagtt gaagcaggac aaaaattgct ggaatttgac ctccaccaaa
    27601 tcaaagcagc tgacctaccg gtcatcactc caattatcgt gaccaataca gctgactatg
    27661 aggacatctt ggtaacccaa gaaagccaaa tcaacagtgg tgattacctg ttgacaacgg
    27721 tcaaataaaa aataaagtgg ttggcagtgc cagtcacttt tttgaatctt gtttagcgtt
    27781 gaagaaaatc cttatcctgc tctggaatgc ccgcctcatc acttttatag cgttcagcgg
    27841 tgacagttag ctggtactta ctacgtaagt ctaggatctc ctgcatcatt ggagaagcat
    27901 ggtggatgtc caaagctgct tggtctgtcc agctatcaac caagaggatt gaatgaggat
    27961 catcgagagg tgcaaagtat tgataacgtg tatttccagc ctgctggcga atgcggtcgg
    28021 caatgccact agcttccatt tcctgagcga aagcacgtgc attgtcagct ggtccggtgt
    28081 agtagaggtg catggtaatc atagtataag atatagaggg cgctaagcgg ccacagacaa
    28141 aatatagtct tttagtttac ttttagtact aggcaatgag ctgcaggcag tactgaagta
    28201 cggcaaagcg aatgaacgaa gtattaaaag aaaaactaaa tgacgtagga aacagaacga
    28261 cgatgcaagc gtccagttct cgtttatctt tttggtttag tccgtagccc gtattcagtt
    28321 caactaaata cagaggccct agtcctttct attatttatc aaagttagta caggtcctat
    28381 tcttccgcaa ttgtttgcga tagctgctag gggtggtatg gtagtattgt ttgaactggc
    28441 ggttgaagtt ggacaggtta ttaaagccaa catcggtcgc aatttcaagc acaggcttga
    28501 tagtgtttgt caggagctcg gtagctgctc gcagtcggac ttggatgata aattccatac
    28561 aggaagttcc tgtttgttgc ttgaagattg tcataaaatg ggtttttgta tagcccattt
    28621 gctctgctag ctgttcgatg gtgatatttt ctgcataatg ttgttggatg tattcaatca
    28681 acaaccgcag tttttcattt ttccgataca tatcatcggt attttttcgg acgatgtagc
    28741 ggtagcggta gagcaggtag aagaagtcaa agagtcggct cttgagaggt agctcaaagt
    28801 aaggcggctg gtgtctgacc aactcaaaaa tggtgagcag gcattgtcta atctctgcgt
    28861 agccaggatc ttggggctgg atgcgtggaa tacacttgaa aacatctgtc tgtaagggtt
    28921 gtaggtagag catactggtc tgatctaggt gagacgcccc cagcatatcc aagtgaaaga
    28981 gaagggtatc agtttcgtgc ccccgttgct caatcggatg aatagaatgc gcagcattgg
    29041 gtcgaatcag gataatatca ccagcttgac tattgaaata gtcatagtcg atatggtaac
    29101 gggctgtccc tccgtaaaca tagttgattt caaattcatt gtgccaatga aagagaatgt
    29161 caggaatgcc atccgtgaca taggtattgt aatacttata gggtaacaaa cgactatcgt
    29221 agtcaatggt atgtttaaac tgtttatccg ttaatggcgg ttgcatactt tcctcctttt
    29281 cgtaggatag tatcattata gcatattatt tgagaagaaa agggtttcaa atctgtttat
    29341 actatatttg ttagaaaaaa ataagggaga agaaacatgt cacgttttcc aaaaaatttc
    29401 ctctggggag gagctatcgc agccaaccag gcagaaggtg cctataatgt ggacggacgc
    29461 ggtctagtcc agacagacgt tacaacagga ggatctgtta acagcccccg ctatacgact
    29521 tacattgata aggacggcaa gccaggtaaa tatgcttcta tggggcatgc tggcggtcat
    29581 ctgccagaag gggcgaccta tgccgtccta gaagaccact attatccaaa ccacaaggct
    29641 gtggacttct accaccgcta taaagaagat atcaagctct tcgcagaaat gggttactcc
    29701 gttttccgct tgtcaatttc atgggcacgg attttcccaa acggtgatga tgcagagcca
    29761 aaccaagcag gtcttgattt ctatcgttct gtttttgaag aatgccgcaa atatggcatt
    29821 gagccattgg tttccatttg gcacttcgat acacctttga gcttggaaca acgctatggt
    29881 ggttggacca atcgtaagtt gattgatttc tatgtccgct atgctgagac aatttttaat
    29941 gaatataagg ggttggtgaa atattggatt accttcaatg agattaacaa cactatcatg
    30001 tttttggata tgtttagtga accagaggat tctgattacc aagaagccta ccaacactta
    30061 catcaccagt ttgttgcctc tgccaaggct gtaaaattag gtcatcaaat tgatccaagt
    30121 tatcgattcg gcaacatgat ttgtggaatc accttctacc caggaacctg tgaccctgcg
    30181 gatattttag aaaatcaaca caaatgggaa cagaatattt tctattgtag tgatgcacaa
    30241 gccaaaggtc gctatggttc ctatgcaaac agactgtgga aggaacacaa tgttgtattg
    30301 caaatggaag agggagacct agaaatcctt gctgaaggca aggtggatat gtataccttc
    30361 tcttattaca tgtcaacagt tgtgaccact cataaagtgg aagacacggt ttctggaaac
    30421 ttctcaatcg gtgccaagaa cccttacttg acctattcag actggggttg ggctcaggat
    30481 ccgaccggtc ttcaatacta tcttgagaaa atctacgacc gctatgaaat tccgatgatg
    30541 attgtagaaa atggtttggg agctttggat acagttgaag aagacggctc tattcacgat
    30601 gattaccgta tcgactacca tcgcgcccat gtcaatgcta tggcagatgc tattgccaat
    30661 ggtgttgatt tgattggcta tacgacttgg ggctgtattg acctagtatc agcaggaaca
    30721 ggtgaaatgc gaaaacgcta cggctttatc tacgttgata tggatgatgc tggaaatggg
    30781 acacttgcta gaacaccaaa agattcattc tactggtaca agaaagtcat tgccagcaat
    30841 ggtgaggatg tagaatagga aaacggcaat gtgccgtttc agattgaaga agaagtccat
    30901 tttggacaat tttcttcaat ctttttttct ttatgatgaa aattaaaaac tctcagaaac
    30961 gccgaaatat caatgttcct aagagtttaa tgacttgtta tctttcacaa acttcttcaa
    31021 ttttttatat ttttaggagt ttgtctaggt tctgagacgg cactttgctg ttttctctta
    31081 gggaaaggtt tgaaaatgaa aatagaacat gtagcaatct acactcagga tttggaaggt
    31141 atgcgtaatt tctttgaaaa ctacttcaat gccacatcca atcaactcta ccataatcta
    31201 aaaaccagtt tcaagtccta ttttcttacc tttgaagacg gggttcgttt ggaaattatg
    31261 accagagacg atgtggtcga caaaccaagt caactcaatt atctaggact tattcattta
    31321 gcctttagtc tagggagtga ggaagctgtg gatgagttaa cagagcgact tgttgctgca
    31381 ggctacttgc ttcttagtgg accacgaata actggggacg gctactatga atcgtgtgtg
    31441 ttgggatttg atgatataca aattgaatta acagtgtaat gttggcaagt aatactgcta
    31501 gatcaatgtt taacggatta gcttgcaagt atcattcatt agcgatttcc ttactgttac
    31561 ggaaaaaact gcaatggtga ttgatgtaaa cgtttgctaa actaataact ataggaacct
    31621 gtattcacaa gtagggtgct tgtatataag agatgttttt gagggaatac tgactgtatt
    31681 ttgaaaaaaa actaacaatg agtagcttaa aaattagtct tagatggaaa ttctgaactg
    31741 taaatacagg ttctaaaata tacaggaggc aattatgtct aaattaccta aaaatttttt
    31801 atggggtgga gcagtagcag ctcaccaact tgaaggtggc tggcaagaag gtggaaaagg
    31861 gatttctgta gcagatgtga tgactgctgg gagacacggg gtacctagag aaattacagc
    31921 tggcgtatta gaaggaaaat attatccgaa tcatgaagcg attgatttct atcaccgcta
    31981 taaagaagat attgcactct ttgctgagat gggcttcaag tgtttccgta cttcaattgc
    32041 atggacacgt atttttccaa agggcgacga actggaacca aatgaagctg gcttgcagtt
    32101 ttatgataac ttatttgatg agtgtttaaa atatgggatt gaacctgttg ttaccctatc
    32161 acactttgaa ttgccttacc atctggtaac tgagtatggt ggttttagaa atcgtaaaac
    32221 cattgagttc tttgttcgtt ttgcagagac ctgcttccgt cgttacaaag ataaggtcaa
    32281 atactggatg acctttaacg agattaataa ccaagcaaat tatcaggaag attttgcacc
    32341 ttttaccaac tcaggtatcg tttatcaaga aggtgaaaat cgtgaagcag ttatgtatca
    32401 agcggcccat tatgaattag ttgcctctgc acgcgctgtt aaaattggac atgaaattaa
    32461 tcctgacttt caaattggtt gtatgattgc catgtgtccg atatatcctg caacctgtaa
    32521 accatcagat attttgatgg cacagaaggc gatgcaaaaa cggtattact ttgcagatgt
    32581 tcatgtgcat ggtttctacc ctgagcatat cttgcgctac tgggagagaa aaggtattag
    32641 cgttgatttc acagaggaag atcgcgaaga tttgctagaa ggaacagttg attacatcgg
    32701 tttctcatac tatatgagtt ttgctattga ttctcaccgc gagaataatc ctcattacga
    32761 ttatcttgag acagaggact tggtgaaaaa tacctatgtg aaggcttctg attgggaatg
    32821 gcaaattgac ccagaaggat tgcgctatgc attaaactgg tttacagata tgtatcatct
    32881 gccacttttt atcgtagaga atggctttgg tgcgattgac caagtcgaag aagatggaat
    32941 ggtgcatgat gactaccgta ttgattatct tggtgctcat atcaaagaat ttatcaaggc
    33001 tgtcgatgag gatggagttg acttgatggg atatacgccg tggggttgta ttgacctggt
    33061 gtcagcaggg acaggtgaaa tgcgcaaacg ctacggtttt atctatgttg ataaagatga
    33121 tgaaggaaat ggtagttatg cgcgttctcc aaaacgctca ttcgcatggt acaaaaatgt
    33181 catcacaaca aatggtgagg aagtttaatg agaagacctc tgtagcgtaa tagctacatg
    33241 ggtcttcatt tatgttttta gtattggtat attaaaggag aaaattgtat ggttggtttt
    33301 atcgcagctt gcttaactac ctttggattt gtacctcagg ttatcaaggt agttaagaca
    33361 aaggatacag agtcgatttc gttaggaatg tatatgatgt ctgtggcagg gatgtcctta
    33421 tggttgttcc atggtattat tcaaggagat acggccctca ttattgctaa tagcctatct
    33481 gtaacattag caggtattat tttggtctat aaattagtct acaaataatg cactattcca
    33541 tcgataaaag aatttttagc aacagtgaaa aacattgaaa atgcttgact ttttctgtga
    33601 aaggaatata atgatgacaa ataaatattg gaagtgtgat tactatgtta ttcgttccgc
    33661 tttttttaag gtttaacctt tttaaaccac caagataagc gttttttcta gttgtttgta
    33721 agtgtatcgt atcttggaaa agaggtttat tatggaatct atagttggaa tagttgtcat
    33781 tgtagttgtt acggttgttc tggtttattt attaactaat atgtggaaaa tcagagcggt
    33841 ttcagttggt caaacggatg acactacgct atactttgta agatttaagg gggtaaatcc
    33901 gccagactta gcaaatagta gtgtttttaa tcaagtagcc aaaatcaaat gtgctaaagg
    33961 agatacctat caagtcactt caacagtcaa tgatgtgaaa ctgagagacg tgattatgga
    34021 tgaatttcag ctcaaatctt ctgaggttgt agtttactat cctcctcgtc ttttctttta
    34081 tgcatcatag tcgcaactct tcggagttgc tatttttttg tttctggaga gtagcggaaa
    34141 agcggtttat tttagtagcg aaatttccgt agtggtaagg aaatacttcc tatggaaaat
    34201 gtaagcgtgt tcatgtaaaa tgaaatcata acaaaagtaa ataataaaat agttggagga
    34261 aaatcctatg gtaaaagctt tgattatctg tgcaggaggc atgtcttctt cacttattgc
    34321 taaaaaaaca caaacacttt tggaagaaca aggtcataaa gtagaaatga acgctgttgg
    34381 tgttccagaa ggtggtaaaa aaattaatgc ggcagagtat gacttgtact taattagccc
    34441 gcagacaaaa atgcacttta agcaatttga agaagcaggt aagaaggttg ggaagccaat
    34501 tgtccaaatc ccaccacaag cttatatccc aattccaatg ggaattgaaa aaatggctaa
    34561 attggtcttg gataatatct agttctttgt taccatagat ggtatattga ttcgaaaaga
    34621 ggatagttat gctaagtact aaggaaaaaa tgatattgca gtatctttac caacaccaga
    34681 atgttttttc aaccagtaaa gtattggcag agcatctctc ttatacagat cgcacaattc
    34741 gaacttatat aaaaaaaatg gcatccgaaa tatctgagag agaaacaggt tttgcgattt
    34801 tatctaaaca aggttatggc tatcagcttc gaattagcga tgaggaaaaa taccatcgtt
    34861 tcttatctga aaaccaacta gtatttgggg tggattatag tgatgcagaa aatcgccata
    34921 aacaaatcct gaataaattg atatttgaag aagaatgtat cttatttgat gatttggcgg
    34981 accagttgtt tgtcagtaga tcaaccttgt ctcatgattt caagcacatt agggagcatc
    35041 ttgcttctta tggtttgagc atcgagagta agccctataa gggagtctat gtagttggtg
    35101 aagaacgtca aaaacgtcac ttcatcatgg actatttctt tagtgagcat ttttatcaga
    35161 atatccatca ttttttatcc aatgataaaa gtctagcctt acctctatct tttgaagagt
    35221 tgacaattgt agtcttagat gagtgtcgtg atagtcagtt gaaactatct gattatatta
    35281 tccaaaattt agttattcat ttaggtttag ctatccaacg ctatcaaaaa ggttttacaa
    35341 tttctcctgt caccatcgat aaggaaaagt atgctgaaga attggcagta gctacaaaga
    35401 ttgctaggcg attatcccat cgactggagc ctcagaatag gtttcctgag gaagaagtgg
    35461 tctacattgc tctacacttg atttcaaaaa cacaattgga ggtggagaag tcatcggacc
    35521 gcctcttgtt gcgccaagca ctttttgaag ttttagggcg ttatgatgat gaatatggtt
    35581 atcaattttc acatgatttt tccttgatag aaggtttgct aacacatctg gaggttctcg
    35641 tagaacgact ccgtcacaat ctccatattg ataaccccct acttgatgat attaaaacaa
    35701 cttatcatga tatcttcgat ttgacacaat cactaatggc tgaactttcc gtttttcact
    35761 cttatagctt gtccgagtca gaaattgcct atgtagctct acacttgatg gcttccttgg
    35821 aacggcaaaa agaaaaatac aaactaaatg ttctggttat ctgtgcaaca ggctacggta
    35881 gtgctcagat gctgaaaaat aggattaaaa atgaacttgg gcaacaggtg tccatatcag
    35941 atgtcatcgg ttactatgat ttagataatc aaaagttgca gggggtagat gtgataatgt
    36001 caaccatcga cttatctggt ttggtgttta aggtccctgt tataactgtc agtgtctttt
    36061 taacggatca ggaagttaag gaagttaaac aacgattggc ggacttgaga cctagtctcc
    36121 aaaaaacaca ggtagtggaa agagagagta acttggagaa atactttgat cagtacttct
    36181 cagaacaacg atttttagtt gttgagtcag ctgaaaaaga aatgttatta aagcaaatga
    36241 tttcacagtt ggagagcgaa ggtcttggaa atcaccagga attttataaa ttaattgagg
    36301 cgcgtgaaag cttgagtaca ttggtatttg ataaagatat agctgttcct caccccctaa
    36361 aacccattag cgaaaagcac atgatttctg ttgcggttat aaaaaatggt ctatattggg
    36421 aagaaggcta tgggcagatt agattggttt tcttagtgag tccatctata tatacaaatg
    36481 aaggtctatc aaccattacc aatcgattgg tagatttggt agatttacca gaagtcaaga
    36541 aagacataat aaactctcct gattttgaaa cattcaaaca cgttttttta caaagagaat
    36601 agaaggtgac atatgaatac agaagaatta caaatggctg catttgggat catcctcagt
    36661 agtggcaatg caagaagtgt tatccacgaa gcttttgctg ctatgagaga aggtgattat
    36721 gataaggcag aagagttatt ggaagccgcc aatgctgaca tgcttgaagc ccaccattct
    36781 caaacgggtc tcctacagga atacgcaagc ggtactgaag tgaaggtaga gattattatg
    36841 gttcatgcgc aagatcattt gatgacaaca atgactttac gtgaggtagc gcttgaaatg
    36901 ttagctttat atcgtaaagt tggttagaaa gaggatatgg gatgagtaag ggaatggcaa
    36961 gtaaaaagct acagaaaaca ccagaacaat taaaacaaga gtctaaacgc aaaagtcagc
    37021 tctttaatcg ctatatgctg tttcgttata gtttagcagt gttcttcttt gcaaatctat
    37081 actggcttct ggttcaatta tttcaaccaa gtttgtatct aatcctgccc tcgcttatgt
    37141 tgattgcaat aatcattgcg acggcagaac aatttaagtt gtatagtgct gaggagtctc
    37201 gattgacaaa gacagaattt gtattaaggg cacaagcaat cttgcaaggg ttaattgtgg
    37261 ttttagtgat tttgggtcat atctcaaccc tctttccaac tttttcaaac catacatctg
    37321 ctaaactctt tgttattggc ttgcagttgt taggtattgc tttagttgtt ctcaatttaa
    37381 aaaggttgga gcaggtaaag caaaataccg ataaatacta cctacgtttt caaataataa
    37441 gaaaatatct ttaaaatttt aggaggtctt ctcatggaag aacaaaaagg gttctttgga
    37501 ctattagata aatatctgat gggaccaatg ggtaaactgg catcttacaa agttgtacgt
    37561 gccatcacag ctgcaggtat ggctgccgta ccatttacga ttgttggttc gatgttcttg
    37621 gttttcagta tcttaccaca ggcattttca ttctggccaa tcgtttcaga tatttttgcg
    37681 gcatcttttg ataaatttac cccactctat atggttgcca atactgctac tatgggggtg
    37741 ctatcacttt attttgcact tgctattgga tatgaattga caaaaattta cgctgaggaa
    37801 gaagaactta atttgactcc gcttaatggg gcgctcttat ccctctttgc tttcatcatg
    37861 accgttcctc aaattatttt tgaagaaggc gcgatgtcgg cagtcagctc tcttgaagaa
    37921 ggcgcagtaa ttgttgatgg ttgggcaatg ggtggtggcg taacacgctt tggtacaaca
    37981 ggtatattta cggcaatcat catggctgtt gcggcagttc tgctatatcg gatgtgcgtg
    38041 aaaaataatt gggttatcaa aatgcccgat tcagtaccag agggtgtatc acgcggattt
    38101 acagctttga ttccttcatt tgtcgtagcc ttctttgtaa ttttcttgaa cggtgtattg
    38161 gttgcgcttg gtactgatat atttaaagtt attgcaattc catttagctt tgttggaaat
    38221 ttaacaaata cttggattgg tttagttatt atctacttgt tgactcaggc actttgggta
    38281 gtaggtattc acggtgcaaa tatcatcttt gcttttgtta gtccaattgc acttgcaaat
    38341 atgtctctta atgcagctgg tgaacgaatg attgttgcag gggagttctc aaatatgttt
    38401 gtcattgcag gtggttctgg tgcaacactt gggctatgta tttggttagc tactcgtgct
    38461 cgttctgaac aattgagtgc gattggtaaa gcagcaattg ttccgggtat ttttaatatc
    38521 aatgaaccat taattttcgg tttaccaatt atttataatc ctgctctagc aataccattt
    38581 atgcttgcac caattgcatc tatgacggta tattacttct ctatgaagtt gaatcttatc
    38641 aatgcagttg ttgctcaagt accatggcca actccagttg gtattggtgc cttccttggt
    38701 acagctgact ggcgtgcaat tgttgtttca attgtttgtg cagttgttgc cttccttgtt
    38761 tactatccat tcattcgtgc atacgataaa cagttgctaa aagaagaagc ggcaaatgct
    38821 taaactagag aaactccttg acttcttcta agtccaagga gtttttctat aagagaatat
    38881 caggagacat tgttagataa ac
//
LOCUS       DAZQCJ010000006        38194 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000006 DAZQCJ010000000
VERSION     DAZQCJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38194
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.006"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            71..493
                     /locus_tag="ACHU66_000318"
     CDS             71..493
                     /locus_tag="ACHU66_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388633.1"
                     /translation="MKEFKLPSIKWHDITSYFTRPKLEILSLIITLICALSVFTGRIA
                     SKQAMTFNNGALQYNGYVVANKMNGQGKLTFDNGDVYEGQFTNGIFHGQGTYTSASGW
                     VYTGQFKNGYADGKGKLTTEGQATYEGTFKQGIYQYEN"
     gene            483..2282
                     /locus_tag="ACHU66_000319"
     CDS             483..2282
                     /locus_tag="ACHU66_000319"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR3388634.1"
                     /translation="MKIKWLSVIRVIGLVFVLLYHFFIKYFPGGFVGVDLFFTLSGYL
                     TTALLIDEFAKHKKIDIVSFFRRRFYRILPPLVLTILLVLPLALLVRNDFIANIGNQI
                     AAALGFMTNFFEILSGGSYENQFSPHLFVHTWTLAIEVQYYLVWAGLVWYLTRISKSV
                     GQLRGSIFLTSISLFSLSFLAMSISSFFVDNFSTIYYSSFTHTFPFFLGSVLATIAGI
                     GNTTTAFQQQVNTLSIKQAIGLFLGGLGVELLLLFTLQFSSIWTYLIGFLLSSLATAT
                     MIFAARVLHEKTEDVKEPAIILFFASISYGIYLFHWPLLIIFTQLTNATIAAGLSFVF
                     STILSTISFYILEPYIAGKVGSLFGLPIDLKPYKKWLLGSFACLTIVGLGICLFAPKL
                     GSFEQESMVSNLYQAQTQLNTTRSAAENAKATSFEIQKGVIIFGDSVTVRASSAIQTA
                     LPDAQIDGTVSRNLSEIAKLINLYKQNNSLKETVVVALGTNTTDNYQELLDQLVKDFP
                     KGRRLIFVTPYDGNFTPSESISYQTGQYEKELAEKYDYISIADWYQVAKDNPPIWYNT
                     DLVHFNLESNGGEVFAQTIKNAVEAVADGPIKN"
     gene            complement(2506..3159)
                     /locus_tag="ACHU66_000320"
     CDS             complement(2506..3159)
                     /locus_tag="ACHU66_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFR3388635.1"
                     /translation="MKNKQIHLRNASFFALAFVILGYVVKFYPSTLAGFDSTVQSAIR
                     GNLPSGASQFWTSITVLGNTVIILAITLILAAIFYYYKKWKIEAYFLLASFAAMGVAS
                     TALKYVYQRPRPSIEWLIDTIGYSFPSWHTASTMMIVGAVVIIVHQRMKKGIARLVLK
                     AVLILLAVLVAVSRIYIGVHYPTDIIGGWLLAVTLLQMMYPFYDQKRFEWRFQSKQK"
     gene            complement(3184..3741)
                     /locus_tag="ACHU66_000321"
     CDS             complement(3184..3741)
                     /locus_tag="ACHU66_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR3388636.1"
                     /translation="MTNTRKMTIIAILSAVSFLLMYLKFPLIPTASFLEVDFSLVPIL
                     FGLLVLDMKSSFAILLVRTFLKLILNNQGPSTIIGLPMNIAAMSVFILAVAYFWKNEQ
                     TLKNYLKAAAVATIGSTAVMLVLNYVYAVPVYAAFANFDIKEILGLTNYLLMMVLPFN
                     LLQGIVLSAIFYICYKAAQPILKKV"
     regulatory      complement(3801..4015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(4065..4610)
                     /locus_tag="ACHU66_000322"
     CDS             complement(4065..4610)
                     /locus_tag="ACHU66_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HFR3388637.1"
                     /translation="MNIETLEYIEETAKNHIVLFEPQIPQNTGNIARTCAATNSPLHI
                     IKPMGFPIDDRKMKRAGLDYWDKLDVRFYDNLAEFMDYASKQGRVHLVSKFADKTYSD
                     EDYNDGNIHYFLFGREDKGLPEDFMREHPEKAIRIPMNDEHVRSLNVSNTACMIVYEA
                     LRQQQFAGLDLVHTYDGDKLK"
     gene            complement(5178..5417)
                     /gene="yidD"
                     /locus_tag="ACHU66_000323"
     CDS             complement(5178..5417)
                     /gene="yidD"
                     /locus_tag="ACHU66_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR3388638.1"
                     /translation="MTRLLIGLVRFYQKFISPLFQPSCRYRPTCSAYMIEALQKHGLR
                     GFLMGLARIGRCHPFVEGGEDPVPDRFSLKRNKQK"
     gene            complement(5417..6148)
                     /locus_tag="ACHU66_000324"
     CDS             complement(5417..6148)
                     /locus_tag="ACHU66_000324"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFR3388639.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELGTVIKSG
                     DKVEVEGKPIYNEEKVYYLLNKPRGVISSVSDDKGRKTVVDLLPTVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYLARVKGVANKEVLRPLTRGVVIDGKKTKP
                     AVYEIIKVDPVKNRSVVELTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGNLDLTGLRP
                     GEYRKLNKKDISKLHTLAVTKPVKK"
     gene            complement(6138..6707)
                     /gene="scpB"
                     /locus_tag="ACHU66_000325"
     CDS             complement(6138..6707)
                     /gene="scpB"
                     /locus_tag="ACHU66_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFR3388640.1"
                     /translation="MNRLAEIEVLLFVAGEDGLTLRNLAEMLEMQPTAVSQQLEKLSE
                     KYMADKASGLAILESSNRYKLVTKKEYANLLRIYAKTPINQTMSRALLETLSIVAYKQ
                     PITRIEVDDIRGVNSSGAISKLQAFDLIRENGKKEVLGRPNLYVTTDYFLDYMGINSL
                     EELPDVSEIDLIQEETELFVERNELENEN"
     gene            complement(6691..7410)
                     /locus_tag="ACHU66_000326"
     CDS             complement(6691..7410)
                     /locus_tag="ACHU66_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFR3388641.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQVDIYEVPITEVIEQYLAYIAT
                     LQAMRLEVAGEYMLMASQLMVIKSRRLLPKVVEQIDPEDDPEMDLLDQLEEYRKFKLL
                     SEKLGEQHDERANYFSKPKLDLIYDDVQLAKDKTIIDIFLAFSKVMAEKQASLRQSHA
                     TIARDEYKIEDMMDFVRSRFETGPRLELRQLFQESQDVNEMITIFLATLELVKVHEIV
                     LEQTETFGDIYLVRSEDESLS"
     gene            complement(7410..8141)
                     /gene="xerD"
                     /locus_tag="ACHU66_000327"
     CDS             complement(7410..8141)
                     /gene="xerD"
                     /locus_tag="ACHU66_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR3388642.1"
                     /translation="MKQAIESFIQSKKVSVNSQKSYTYDLQQFVTVTKGEISQQSLLV
                     YQQSLLDLKPAAQKRKMSAVNQFLYFLYENNLLDRFYKLQTMSEPASVKKKLEREDLT
                     LLFQESPWLDGQLITLLIALLGLTPSEIAELTSQQVNLDFQVLTVEKGGAKRVLTLPK
                     ELIPYMESHLSGRYVFDKKGQTYSRQWFFNRLTEFVQSIGKPDWTAQKLREQYILKHM
                     DEGKSLDQIAKQLGLKTSMSLEKFR"
     gene            complement(8138..8596)
                     /gene="cbpB"
                     /locus_tag="ACHU66_000328"
     CDS             complement(8138..8596)
                     /gene="cbpB"
                     /locus_tag="ACHU66_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFR3388643.1"
                     /translation="MIAREFEVFLLDQEGTFLTPADKLAVIIDTHNIDHAKLLLSHMT
                     YSRVPVVTEDGRFFGTIGLTEIIKYQAENELSDDELNRDISVIVKTDEETVGLDYDLT
                     EVMRKLIDQSFLPVLGENREFLGIITRKSILKAINALLHNFPLASKEGKK"
     gene            complement(8593..9120)
                     /locus_tag="ACHU66_000329"
     CDS             complement(8593..9120)
                     /locus_tag="ACHU66_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR3388644.1"
                     /translation="MAGASKTILVMSDSHGDRQIVEDIKHHYLGKVDAIFHNGDSELD
                     SQDSLWDGIQVVNGNCDYFGGYPDQLITQLGDVTIAQTHGHLYGINYGWQRLDYWAQE
                     VDADICLYGHLHVPDAEVRGKTLFLNPGSVSQPRGLVRECLYALVTIYDDHFHVDYYN
                     RQHQLYPALTKDISR"
     gene            complement(9093..10103)
                     /locus_tag="ACHU66_000330"
     CDS             complement(9093..10103)
                     /locus_tag="ACHU66_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HFR3388645.1"
                     /translation="MTDKIYEYRDEHNWFIGKASFANLFGSFGENGREQEIYQIGQLF
                     DKLIAGNYEDENFNQCVNIEVIKLQSEFALFQFACDTLNELNNRQFKVLQHQGAILVT
                     ENDKLLLVHLPQAGVSTADFFGQDKGLASVGDTILIATKNEGKTKEFRKFFERFGYQV
                     ENLNNYPDLPDVAETGMTFEENARLKAETIAELTGKMVLADDSGLKVDALGGLPGVWS
                     ARFSGPDATDELNNAKLLHELAMVFELKDRSAQFHCTLVMAAPNRDSLVVEADWEGFI
                     GMDLRGEDGFGYDPLFLVGETGKTSAELTLEEKNKISHRAQALEKLVEAFPVWQEQAK
                     QS"
     gene            complement(10100..10894)
                     /gene="racE"
                     /locus_tag="ACHU66_000331"
     CDS             complement(10100..10894)
                     /gene="racE"
                     /locus_tag="ACHU66_000331"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR3388646.1"
                     /translation="MDNRPIGFLDSGVGGLTVARELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKNVKMIVFACNTATAVAWEEVKEKLDIPVLGVILPGASA
                     AIKATQTGKVGVLGTAMTIQSDIYRQKIQALSPETQVDSLACPKFAPLVESNSHQSSL
                     AKKVVYETLRPLVGKVDTLVLGCTHYPLLRPIIQNAMGKDVKLIDSGAECARDISVLL
                     NYFQINRSRTEKDIQHRFYTTASPAAFKEIAESWMGIDIHVEHVEL"
     gene            complement(10984..11220)
                     /locus_tag="ACHU66_000332"
     CDS             complement(10984..11220)
                     /locus_tag="ACHU66_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFR3388647.1"
                     /translation="MNLGLAILLIVLAFAGGVALGIYLSRKQVENYIADKPVLDENAL
                     RLMMSQMGQKPSEAKVQQVLRQIKSQQKVASKKK"
     gene            complement(11323..12570)
                     /locus_tag="ACHU66_000333"
     CDS             complement(11323..12570)
                     /locus_tag="ACHU66_000333"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR3388648.1"
                     /translation="MTKTPFVSKEVLETITEQFPTPFHLYDEKGIREKARALNAAFAW
                     NKGFKEYFAVKATPTPAILKILQEEGCGVDCATDVEVLMSEKLGFKDIMFTSNDTQAQ
                     EFVYARKVGATINLDAYEHIEFLKNVAGIPETVCLRYNPGGVFSLGTDIMDHPEESKF
                     GMTKEQLMKGYKELKELGVKEFGIHAFLASNTVTNDYYPVLAHQLFELALEIREETDV
                     TLDFIDLSGGIGVNYRPEQEPNDIAVIGEGVRKVYEEILTPAGMGHIKIFTELGRFML
                     APHGHLITKVLHRKETYRTYIGVDASAANLMRPAFYGAYHHITNITRPDAPIEVVDVA
                     GSLCENNDKFAVNRELPRAEVGDTLVIHDSGAHGFSMGYNYNGRLRSSEILLQEDGTA
                     RMIRRAETPEDYFATIYGFDFDR"
     gene            12704..14035
                     /gene="pepC"
                     /locus_tag="ACHU66_000334"
     CDS             12704..14035
                     /gene="pepC"
                     /locus_tag="ACHU66_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HFR3388649.1"
                     /translation="MSTLDFDFTERLYANYLANPSLQATENAVSHNGLLKSLETRQSA
                     IDNDYVFSIDLTKDAVSNQKASGRCWMFAALNTFRHKLISDFKLENFELSQAHTFFWD
                     KYEKSNWFLEQIIATADQEIGSRKVKFLLDTPQQDGGQWDMVVALFEKYGVVPKSVYP
                     ESISSSASRELNQYLNKLLRQDAQILRDLLAKGASSKEVQIQKENLLQEIFNFLAVNL
                     GLPPRSFDFAYRDKDNVYHRNTNVTPQAFYEKYVGLKLSDYVSIINAPTTDKPYNKSY
                     TVELLGNVVGAPAVRYLNVEMNRFKELAIAQLKAGESVWFGSDVGQSSNRQTGIMATN
                     TYDFSSGLGIHFHQDKAGRLDYSESLMTHAMVLTGVDLDDNEQPLKWKVENSWGDKVG
                     DKGYFVASDSWMDEYTYQIVVRKEFLTQEELAAYQAQPQVLAPWDPMGALA"
     gene            complement(14085..14786)
                     /locus_tag="ACHU66_000335"
     CDS             complement(14085..14786)
                     /locus_tag="ACHU66_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR3388650.1"
                     /translation="MNNDSFIINQVDNTALNRFFGKIYGVVAMGIGLSALVSFLAVTV
                     FQSLLLSLLSAGSIIMMLIMIGQIALVVSASAMAAKNSPMALPMFLAYSVTNGITISM
                     ILMFYTSETVVLAFVSAALMFAIMAVIGMTTKKDLSGMAQALRAALWGIIIASVINIF
                     LRSSGLSFMMSIISVLVFSGLIAYDNQRIRNVFEQTGGNVGQGWVVSMALQLYLDFIN
                     LFLNLLRIFGGLSRD"
     gene            complement(14808..15290)
                     /locus_tag="ACHU66_000336"
     CDS             complement(14808..15290)
                     /locus_tag="ACHU66_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFR3388651.1"
                     /translation="MVAYKQDKEYMDYVGHLIATPKVQKLGKIPHHYYSTRLEHSINV
                     SYTSYKIAKKFGWDAKSTARGGLLHDLFFYDWRDTKFNKSHAWVHPRIAKRNAQKLIQ
                     LNKLEEDIIVKHMFGATIAPPRYKESWIVTCVDKYWAVREWSLPMQHKWKNRKVFRFQ
                     "
     gene            complement(15338..16078)
                     /locus_tag="ACHU66_000337"
     CDS             complement(15338..16078)
                     /locus_tag="ACHU66_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFR3388652.1"
                     /translation="MDIIRSKTNNLVKQVKKLQQKKYRTSSYLIEGWHLLEEAMEAGA
                     NIEHIFVVEEYFEKVAGLANVTVVSPEIMQDLADSKTPQGVVAQLALPSQLLPETLDG
                     KFLVLEDVQDPGNVGTMIRTADAAGFDGVFLSDKSADIYNMKVLRSMQGSHFHLPVYR
                     MPISSILTALKSNQIKILATTLSSQSVDYKEITPHSSFALLMGNEGQGISDLVADEAD
                     QLVHITMPGQAESLNVAIAAGILLFSFI"
     gene            16243..16524
                     /locus_tag="ACHU66_000338"
     CDS             16243..16524
                     /locus_tag="ACHU66_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR3388653.1"
                     /translation="MRKVKMIASGIVQGVGFRWSVQYLAMEIGDIAGRVWNNEDGTVT
                     ILAQSENAEKLSHFIHEIRKGPSRMAKVTYLDVTLANFEDYKDFQVSYR"
     gene            16594..17508
                     /gene="yidC"
                     /locus_tag="ACHU66_000339"
     CDS             16594..17508
                     /gene="yidC"
                     /locus_tag="ACHU66_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR3388654.1"
                     /translation="MKNKRLFMLSGMALSTLLFLSGCVQTDKSGNPTGLIWDWLGQPM
                     SNLIRFFAEDQALGFGLAIIIVTLLVRFIILPLGIYQSWKAAIQSEKMNYLKPILGPI
                     QERMKNASSQEEQLAAQQEYFATQKQYGVSMLGGIGCLPMLIQMPFFSAIYFAARHTP
                     GISEATFLGINLGSTSLILTVVAGILYYAQSLLMQVGMDEEQKKQMKTMALMNPIMIM
                     MFSFGSPAGVTLYWVVGGLFGILQQAITNFILKPRIRKQVEEEFKNITVSATPRKTKD
                     VTPTASAVIEEKTSNKKRNRNAGKQRSR"
     gene            complement(17592..18074)
                     /gene="greA"
                     /locus_tag="ACHU66_000340"
     CDS             complement(17592..18074)
                     /gene="greA"
                     /locus_tag="ACHU66_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR3388655.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEIVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISTIETKIRYAEIVNSDAVAADEVAIGKTVTVQEVGETE
                     EEVYHIVGAAGADAFANKISNESPIGHALIGKKTGDVATIETPAGSYDVKILKVEKTK
                     "
     gene            complement(18148..19965)
                     /gene="mltG"
                     /locus_tag="ACHU66_000341"
     CDS             complement(18148..19965)
                     /gene="mltG"
                     /locus_tag="ACHU66_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFR3388656.1"
                     /translation="MTKDTNEKNTQSSGFRDQILRDLEELKVKRLAEQSADVLVDKRN
                     EVINELQPSLAEEKAPHRETEIVKVEGIAFPDSYLSEEQQESEVMTESIEEPTSETLA
                     TPETFVIEKEVVSSPVPQDTVERNLEKLRNLVAANTAGFDEVPSSSSVSEPVESVLPG
                     NSPLEDTFLEFPTEDVSAVTGDTEIISLEETIIAPKTTAIVPEVDKQMQETQPRRRTT
                     HRTSKQRRKKQDNAAKHIVSVIMSIVVVAVLVTGVTGYMWVKSSLEPVDAKATETIQV
                     EIPEGTSTLEIGKILVDNKLIKNATIFNYYSKIKSYNNFQSGFYNLKQNMSVDDIAKA
                     LQESGTPTAQKEAAGKVLIVEGYTLTQIAQAITDNTNTKDKNDKTPFTAEQFMATVTN
                     QDFINRMVATYPKLFASLPAADSGVIYQLEGYLFPAVYEYSDETTIEELVEQMIAAMD
                     NRLQPYYETITAKNLTVNEVLTLASLVEKEGSTDEDRRNIASVFFNRLNAAMPLQSNI
                     AILYAQGKLGQETTLAEDAAIDTSIESPYNIYWTPGLMPGPVDSPSLSAIEAVINANT
                     TDYLYFVADVTTGNVYFTNNIDEHNQNVAKYVNAHLNNE"
     gene            complement(20037..20522)
                     /locus_tag="ACHU66_000342"
     CDS             complement(20037..20522)
                     /locus_tag="ACHU66_000342"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR3388657.1"
                     /translation="MEIRFATPSDLEQVVLIENANFSKEERIAESVLATYLNTLSKTC
                     LIMEHDGEIAGYLLSCPSVSQTVTDDIFYLTESDMPTGSHLAIASLSVADSYKGQGVG
                     TLLLAAIKEVALAGGFEGVSLTCKGYLISYYEMNQFEDFGPSLSQFGGQRWSDMYWKA
                     L"
     gene            complement(20578..21909)
                     /gene="murC"
                     /locus_tag="ACHU66_000343"
     CDS             complement(20578..21909)
                     /gene="murC"
                     /locus_tag="ACHU66_000343"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR3388658.1"
                     /translation="MTKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGIQILPFDEKNITADVELIAGNAFRPDNNVEIAYVDAQGYTYKRYHEFLGEFMKG
                     FTSLGVAGAHGKTSTTGLLAHVMRNITDTSFLIGDGTGRGSANAQYFVFESDEYERHF
                     APYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQVKKALLVFGEDEQLRRITANA
                     PIYYYGFEENNDFVAYDLKPSTSGSQFKVRHGEEELGEFQIPTFGKHNVMNATAVIAN
                     LYIAGFDLQLVAEHLKTFGGVKRRFTEKIVNDTVIIDDFAHHPTEIIATIDAARQKYP
                     SKELVAIFQPHTFTRTIALLDEFADALNGADAVYLAQIYGSARETDNGQVKVEDLAAK
                     INKKGGLVTVENTSPLLDHDNAVYVFMGAGDIQSYEYSFERLLSNLTNNVQ"
     gene            complement(21911..22573)
                     /locus_tag="ACHU66_000344"
     CDS             complement(21911..22573)
                     /locus_tag="ACHU66_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388659.1"
                     /translation="MIRHEKRFPLVADDEVLVGENPIMSLYDESDLISNIRGLYQEKE
                     FSWSTESQRVASAKPVAQTEDELLPPLFEAKPSHYSRKERLQQLTKTKPSPVKTQGQL
                     AREQAREDLKKKRSATYLRDEKPAPAKVVVKPPVVSTTESKAGRLTRLADNLRQTDYI
                     LADMPAVYSLKKEDREQEQVIKKNSYDFLRKSQVYNYPERRQQRDRQVAQELNLTHIE
                     EE"
     gene            complement(22652..25744)
                     /locus_tag="ACHU66_000345"
     CDS             complement(22652..25744)
                     /locus_tag="ACHU66_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFR3388660.1"
                     /translation="MGRMMPGRIRQEGIDLYEAGKLTVLHSENGKMVLDIAGERFTYG
                     DADSDLQCSCQLFQSKGYCQHLAATEYFLKNDASGKDMKQSLKEDSEQHKETVRRTYF
                     GGLFLDEILRPEPELGLKYQLSVEGSLLPYDRQIDWTLKITRLPDTRSYIVRDIGAFL
                     RIVKANGHYQIGKNYYEQVTYENFDEPSQALLDFLWALVPEKVGIDSDILTHFGRHFR
                     LPQAYFEEGLELLNQLEHFNFSYQQQSYSSLKVLPLTGEEGLYHFEVTVHTQMIEMVI
                     HEKTVRPLFHGRYLLVNGTVYSVNRHQEHLIYQISELVPTESGLRKVQVDFPDQNRLA
                     LSLLDLQTIGTVKAPKRFIIHDFKPEFHIQMEANESLSLQLVLDFDGRKVRSEEDLAL
                     LPFASHFQHLERVYQAIRLAGFRGTYHAQRAALSQQELYPFFQQQLPILQKMGQVHLA
                     ERLQALYIEAKPQLEIVRNGSLLDISFDLSGIEQSEIDQAITALLNQEDHYTSPSGKV
                     FVFDEETKKISQTLIYLRARHGKEGQVQVHALAGYQLAQSLSQFNQVSFSKEFEEMAS
                     YLAQPDLFPLPAVEVTTSLRDYQQTGLKWLTMLDTYGFGGILADDMGLGKTLQTIAFL
                     SSRMTKDSKVLILAPSSLIYNWLDECKRFAPSLDVAVVHGNKEQREEIIANGHQVLVT
                     SYPSFRQDVALYKQERFDYLILDEAQVMKNAQSKIAQLLREFEVGNCFALSGTPIENH
                     LTELWSIFQIVLPGLLPGKQDFGKLAAKDIARTIQPFVLRRHKEDVLQELPDLIEVNV
                     LNELTDEQKAIYLAQLQQMRTQIAGADDAQINRSKIEILSGITRLRQICDTPSLFMEE
                     FSGESGKLNSLKELLLQLKEGEHRVLIFSQFRNMLEKIEEQLVEIGMTSYTLTGSTPA
                     NQRQEMTQAFNAGSRDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAISRAHR
                     MGQTEKVECYRLITRGTIEEKIQELQENKKNLVKTVLDGNESRANLTVEDIREILGIE
                     "
     gene            complement(25884..26192)
                     /locus_tag="ACHU66_000346"
     CDS             complement(25884..26192)
                     /locus_tag="ACHU66_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388661.1"
                     /translation="MEFFGELLVEFLTGLADFDEKKHPPFGIRYWLGWLGVLIHVLLL
                     ALLTCVTVFFFKFFLVGKGLINVVVAVVFLLFALFWLWKSGKTILKMWQATIYYLAIH
                     "
     gene            complement(26194..26532)
                     /locus_tag="ACHU66_000347"
     CDS             complement(26194..26532)
                     /locus_tag="ACHU66_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR3388662.1"
                     /translation="MVAVPIWRSFLILLSFSSFFLVFYEDSQYKPFGFRYWLGLVACI
                     WLNFVTLASYFIAFTGGSIMVYNQYKQPLVVIFLFCLFVASMLTLLSFKAIKMMIRRT
                     KYYRQVRKVG"
     gene            complement(26600..27910)
                     /gene="der"
                     /locus_tag="ACHU66_000348"
     CDS             complement(26600..27910)
                     /gene="der"
                     /locus_tag="ACHU66_000348"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR3388663.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRKFSLIDTGGIDDVDAPFMEQIKHQAEIAMDEADVIVFVVSGKEGVTDADEY
                     VSRILYKTNKPVILVVNKVDNPEMRNDIYDFYSLGLGDPYPVSSVHGIGTGDVLDAII
                     ENLPVQEAEENPDIIKFSLIGRPNVGKSSLINAILGEERVIASPVAGTTRDAIDTHFT
                     DPEGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIERSDVILMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKKWEDDIRDQFQYLSYAPIIFVSALTKQ
                     RLHKLPEMIKAISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVVFVNEEELMHFSYMRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(27929..28642)
                     /locus_tag="ACHU66_000349"
     CDS             complement(27929..28642)
                     /locus_tag="ACHU66_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HFR3388664.1"
                     /translation="MNETIDLMLNHTSVRRFTEEPIEAEHLQAIISAGRAASSWKNFQ
                     SYSIIVVQLEEKKQALYDLVPQPAILQAQAILVFVGDHNRASKAAQLHGSDFDAKGTE
                     NLLISSVDASLAGQNALLAAESLGYGGVFIGMIRHKAMAIAELFNLPDYTYPIFCIAL
                     GRPAQNHPVKPRLASETIVFQEEYVEQGVEVIQAYDQVQTAYAGARQTETWSERMVAQ
                     FGQAEQPETKSILEKNKLL"
     gene            complement(28639..29538)
                     /gene="dnaI"
                     /locus_tag="ACHU66_000350"
     CDS             complement(28639..29538)
                     /gene="dnaI"
                     /locus_tag="ACHU66_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFR3388665.1"
                     /translation="MKSVQDRLSQTTNPSPKSYQQLYQEIVSDPEVAAFIKKEGLTQQ
                     EITLSISKFLEYISQRDLFVKQDEAYIAKGYQPVLVMNEGYADVSYLETEELVEYRRL
                     EAIKNRIQLINMPASLKNVTVADIDKSDENRVEVMLAIADFVKRFEEKPKGLYIYGNF
                     GIGKSYLMAYLANLLSKTHLQSTTMLHYPTFVVDIKNAIKDGSVKERIDEIKMAQVLV
                     LDDIGAEQHSPWVRDDVLQVILQYRMQENLPTFFTSNFSFDDLERHFASGKSGDETWQ
                     AKRVMERIRYLARDLHLKGNNRR"
     gene            complement(29538..30689)
                     /locus_tag="ACHU66_000351"
     CDS             complement(29538..30689)
                     /locus_tag="ACHU66_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation/membrane attachment
                     protein"
                     /protein_id="HFR3388666.1"
                     /translation="MKPNDLFAYIKISPFTPDIVSLSQCYQPIIGFDALALYYYFYSF
                     ADQGQGRYKWTVILNHMDFGMNRLEKALDVLTAMGLLELYRADELTGLALQAPLTVKS
                     FLAKPLYKNLLARKIGESSMENLLVHQPNQEQNISKTFSQVFTMDGQAPVVFEPKHDF
                     DWSAFKALMAKDKLLFQDEAEDIIALSYIAEQAGWTWLETYRQAKATAIGQTISTKRL
                     QQSRQSLPTENANLTAQEQAIVREAKSKSSLTFLSFIKEQRKAAVTGTERKCLTDLAN
                     LGLLDEVINVLVLYTFNKVDSANLNEKYAMKLGNDFSYKGSVSAEAAVLYLRELKTGQ
                     VVTKSSQPQKTNVPEWSKEEVQQGQTQEGQAKLAALYRELEEMENKGGS"
     gene            complement(30676..31167)
                     /gene="nrdR"
                     /locus_tag="ACHU66_000352"
     CDS             complement(30676..31167)
                     /gene="nrdR"
                     /locus_tag="ACHU66_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR3388667.1"
                     /translation="MRCPKCQSLKSSVIDSRQAEDGNTIRRRRSCDQCGQRFTTYERI
                     EEKTLVVVKKDGTREQFSREKIFNGIIRSAQKRPVSTDDIDEVVNRIEQKVRAQGDSE
                     IESDVIGNLVMEELVELDEITYVRFASVYRSFKDVGELENLLKQMISKGSKVKPGKKD
                     ETK"
     gene            complement(31239..32369)
                     /locus_tag="ACHU66_000353"
     CDS             complement(31239..32369)
                     /locus_tag="ACHU66_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR3388668.1"
                     /translation="MNTSFKEKVILILLSGATLLAVLNWSSIYGAIQQLLGSMNSLFI
                     GAIFAFILNVPMKKLEDQFEKISFLKKSKRSLAIVGVLIGFALIVTGLVVIVLPTLVT
                     TVSELVSVSSTAIPKSVNALTNFLESNGLLSGQIGDQIASFLDQLKNLTLISSLVTPI
                     LSGLVSNVTGIFSNTMTLIMAFFFTLAILGSKEHLQAMTRKFLQATLPEKAVRVVNYI
                     GEVIVDTYDRFLMSQIVEACIIGTLVFVSYSLSGIPYASMAGILAGVLSFVPYIGPFS
                     ACLISALFVAVQNPLLALWSIALFQIIQLIEGNVIYPRVVGQSVGLPTLFTLAAALIG
                     GNLFGLLGMVFFTPIFAVVYRLVREWVASRLKKQGELLAESE"
     gene            complement(32459..33148)
                     /locus_tag="ACHU66_000354"
     CDS             complement(32459..33148)
                     /locus_tag="ACHU66_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR3388669.1"
                     /translation="MAKKILIAGKERNLSHFVSMELQKKDYLVDYASTGKEAMSLAHE
                     TDFDLILMSFQLSDMSSKELAAELLAIKPATVMIVVVEPTEVSQYGEEVLSYAVSYVV
                     KPFVISDLVEQISAIFRGRDFIDNNCKQVHMHAAYRDLKVDFQNRTVTRGDELINLTR
                     REYDLLATLMNSPEPVSREQLLERVWKYEAASETNVVDVYIRYLRGKLDLPHQDSYIK
                     TVRGVGYAMRD"
     gene            complement(33230..34657)
                     /gene="gndA"
                     /locus_tag="ACHU66_000355"
     CDS             complement(33230..34657)
                     /gene="gndA"
                     /locus_tag="ACHU66_000355"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR3388670.1"
                     /translation="MTKANFGVVGMAVMGRNLALNVESRGYSVAIYNRSADKTEDVVA
                     SNPGKNLVPSYDVESFVASIEKPRRIMLMVQAGPGTDATIQALLPHLDEGDILIDGGN
                     TFYEDTIRRSKELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGAPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSVDEMAD
                     IFTEWNKGELDSYLIEITADILKRKDDQGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKDERVAASKVLPKPAPFAYEGDKAELVEKIRQALYFSKI
                     MSYAQGFAQLRVASKENNWNLPFGEIAKIWRAGCIIRARFLQKITDAYGRDEDLANLL
                     LDEYFLDVTAKYQQAVRDVVALAVQAGVPVPTFSAAITYFDSYRAENLPANLIQAQRD
                     YFGAHTYNRKDKEGIFHYDWYSESK"
     gene            complement(34754..35293)
                     /locus_tag="ACHU66_000356"
     CDS             complement(34754..35293)
                     /locus_tag="ACHU66_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFR3388671.1"
                     /translation="MFHIYDIQKNLDGISFDKTLDLQEELQARNGEVLGLSPVEVTGN
                     IRFESGFFFLDYQMTYDITLASSRSLQPVVLHEVQNVNELFVANEAVLKEQDLIDEDM
                     VLVVEDDHIVLDESVADNILLAIPIKVLTPEEEAGQELPSGQAWALMTEEDFQQKAQE
                     KKEANSPFAQLQGLFGDED"
     gene            complement(35303..36220)
                     /gene="trxB"
                     /locus_tag="ACHU66_000357"
     CDS             complement(35303..36220)
                     /gene="trxB"
                     /locus_tag="ACHU66_000357"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR3388672.1"
                     /translation="MYDTVVIGAGPAGMTAALYAGRSNLKVALLERGIYGGQMNNTAE
                     IENYPGYDHISGPALAEKMFEPLEKFGVDHIFGTLVRIEEEGQIKKVITEDGVLETKT
                     VVLAMGAKHRLLGIPGEDTYNSRGVSYCAVCDGAFFRGQKLLVVGGGDSAVEEALFLT
                     QFAESVTIVHRRDQLRAQKVIQDRAFANEKINFIWDSVVEEIKGDDLRVQSVVIKNVK
                     TEEVSELDFGGVFIYVGLDPMTDTVADLGITDEAGWVITNEKMETTQSGIYAIGDIRQ
                     NQLRQIATAVGNGAVAGQEVYNYITELAE"
     gene            complement(36234..36458)
                     /locus_tag="ACHU66_000358"
     CDS             complement(36234..36458)
                     /locus_tag="ACHU66_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HFR3388673.1"
                     /translation="MLQNILSFYLQGLLIASLLIITSSLVWFIWRATKGVDKTLQERQ
                     DFLFDLLMINVMTIPIAAFGVVGILLMFKA"
     gene            complement(36476..37819)
                     /locus_tag="ACHU66_000359"
     CDS             complement(36476..37819)
                     /locus_tag="ACHU66_000359"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR3388674.1"
                     /translation="MKFTDMKLKPYIQEALKEINFVQPTEVQEKLIPVVLSGRDLIGE
                     SKTGSGKTHTFLIPIFQKLSEELDQVQAVITAPSRELATQIYQAARQLASHSDIEVRV
                     TNYVGGTDKNRQIDKLQASQPHIVVGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEQIKTHTVISDTIE
                     NWLISTKGRDRNAQILEVTKLMQPYLAMIFVNTKTRADELHSYLTANGLRVAKIHGDI
                     PPRERKRMMNQVKNLDYQYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLSGIAITLYQPSDDADIRELEKLNIKFLPKEMKNGEFVDTYDRDRRANREKSREK
                     LDLEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRVEARAKGRAERKAKRQTF"
     gene            complement(37899..>38194)
                     /locus_tag="ACHU66_000360"
     CDS             complement(37899..>38194)
                     /locus_tag="ACHU66_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="HFR3388675.1"
                     /translation="SHPLVKKVAAVIGVKITKKTVEKTIANAVPILGGLISGGLTYFT
                     FQPMGGMLADTFVKKINGDFDGELELTEAFKASLEEEKELGIIEAEFTEASES"
BASE COUNT        11057 a         8677 c         7183 g        11277 t
ORIGIN      
        1 ctgataattt cttactgtat cctagaaaga actgattagc gtcggtcaga tcctatgaaa
       61 gagtaaaacg atgaaagagt tcaaattacc tagtataaaa tggcatgata taacaagtta
      121 tttcacccgt ccaaaacttg aaatcctctc ccttattatc accctcatct gtgccctctc
      181 tgtatttaca gggcgaattg ccagcaagca agccatgact ttcaataatg gcgccctgca
      241 gtacaacggt tatgtggtcg caaataaaat gaatggtcaa ggaaaattga cctttgataa
      301 tggagatgtc tacgaaggac aatttacaaa tggaattttc cacggtcaag gaacctacac
      361 atctgcaagt gggtgggttt ataccgggca atttaaaaac ggctacgccg atggaaaagg
      421 aaaattgacc accgaaggac aagctaccta cgaaggaact tttaaacagg ggatttatca
      481 atatgaaaat taaatggtta tccgttatac gtgtgattgg attagtcttt gtcctactct
      541 atcatttctt tatcaaatat ttcccaggtg gctttgtggg ggttgacctc ttctttaccc
      601 tgtctggata tttgacaacc gcccttttga ttgatgagtt tgcaaaacat aaaaaaatag
      661 atattgtcag ctttttcaga agaaggttct atcggattct cccccctctg gttttaacga
      721 ttcttttggt tcttccactt gctctgcttg tccgcaatga tttcattgcc aatatcggca
      781 atcagatagc cgctgcgctc ggattcatga caaacttctt tgaaattcta tcaggtggta
      841 gttacgaaaa tcaattttca cctcatctat ttgtgcatac ttggacacta gccatagaag
      901 ttcagtatta ccttgtctgg gctggtttag tttggtatct gactcgcatc tcaaaaagtg
      961 tggggcaact acgtggatca atttttctaa catctataag cctcttctcg ctaagttttt
     1021 tagcaatgtc tatctcaagt ttctttgtgg ataatttctc aaccatttac tactcgagct
     1081 tcacacatac cttcccattc ttcctcggaa gcgttctcgc aaccatcgct ggtatcggaa
     1141 atacaacaac tgccttccag caacaagtga atacattgag tatcaaacaa gctataggtc
     1201 tgtttcttgg aggactagga gtagaacttc ttcttctctt tactcttcaa ttcagttcca
     1261 tctggaccta tctaattggt tttcttctat ccagtttagc tacagcaact atgatttttg
     1321 ctgctcgggt acttcacgag aaaaccgaag atgtcaaaga acctgcaatt atcctcttct
     1381 ttgcaagtat ttcttatggt atctacctct tccattggcc cttgctcatt atctttacac
     1441 aactgacaaa tgccactatc gctgcgggat tatcctttgt tttttctact atcctatcaa
     1501 ctatttcctt ctatattcta gaaccatata ttgctggtaa agtaggaagc ttgtttggat
     1561 tacccataga tttgaaacca tataaaaaat ggcttcttgg tagctttgct tgcttgacca
     1621 tagttggctt aggtatctgt ctgtttgcac caaaattagg aagttttgaa caagagagca
     1681 tggttagcaa tctttatcaa gctcagaccc aactaaatac aacacgatct gctgctgaaa
     1741 acgctaaagc tactagtttt gaaattcaaa aaggagttat catctttggc gattctgtta
     1801 ctgttcgcgc tagttctgcc atacagaccg cgctaccaga tgcacagatc gatggaaccg
     1861 tcagtcgaaa tctctctgaa attgctaaat tgataaatct ttataagcaa aataatagct
     1921 tgaaagagac tgtagttgta gccctaggta caaatactac ggataattat caggaactac
     1981 tagaccagct agtcaaagat tttcctaaag gacgtcgcct tatttttgtc actccttacg
     2041 atggaaactt cactccgagc gaatccattt cctatcaaac tggtcaatac gagaaagaat
     2101 tggctgaaaa atatgattac atttccattg cagactggta tcaagttgca aaggataatc
     2161 caccaatttg gtacaatacc gaccttgttc atttcaatct tgaaagtaat ggaggcgaag
     2221 tcttcgccca aacgataaaa aatgctgttg aagccgttgc tgatggtcca atcaaaaact
     2281 agatttatac atcattatcc tctgagaatt tctcggagga tttttgcact attttcctat
     2341 aagtccagcg cgaactctaa gaaatggcgt tgtgcctgag ctcagcctca ataaatcttg
     2401 tgaaataagc cacttctaca aaggacggca aagcgaagta acgataatag aaagatgaca
     2461 agttaaaact cctacctttt acagtaggag ttggtgtcaa attgactatt tttgcttact
     2521 ttggaaacgc cattcaaagc gtttctgatc gtaaaatgga tacatcattt gtaaaagagt
     2581 gacggcaagt agccaaccac cgataatatc tgtcgggtag tgaaccccaa tatagatacg
     2641 ggatacagct accaggacag caagtagaat caacacggct tttagcacaa gtctagctat
     2701 gcctttcttc atcctctggt gaacaataat aacaactgct cctacaatca tcatagttga
     2761 tgcagtatgc cagctaggaa atgaataacc gatggtatca atcaaccatt caatacttgg
     2821 acgcggacgt tggtagacat atttcagggc tgtggatgcc acacccatcg ccgcaaaact
     2881 agcaagtagg aaataagctt ctatcttcca ttttttatag tagtaaaaga tagcagctag
     2941 aatcaaggta atagctaaaa taataaccgt atttcctaaa acagtaatag aggtccaaaa
     3001 ttgactggct ccacttggta aatttccacg aatggcagac tggacagtcg agtcaaaacc
     3061 tgctagggta ctaggataaa attttactac atagcccaat atcacaaagg ccagagcaaa
     3121 aaaagatgcg tttcgtaaat ggatttgttt attcttcata cagtatagtt taccttataa
     3181 aggttaaact ttctttaaaa taggttgagc tgctttgtaa cagatataaa atattgctga
     3241 aagcacaatc ccttgtaaaa gattaaaagg aagtaccatc atcaaaagat agttagtcag
     3301 ccccaagatt tctttaatat caaaatttgc aaaggctgca taaacaggca ccgcataaac
     3361 gtaatttaat accagcatca ctgctgtcga accaattgtc gcaacagcgg ctgcttttaa
     3421 ataatttttt aacgtttgtt catttttcca gaaataagct actgctaaaa tgaaaactga
     3481 catagctgca atattcatcg gtaagccaat aattgtcgat ggtccctgat tatttaaaat
     3541 cagtttgaga aaagttcgta ccaaaaggat agcaaaacta gacttcatat ctagaacaag
     3601 cagaccaaat aaaatcggta cgagagaaaa atccacttcc aggaaactgg ctgttggaat
     3661 cagcggaaat ttcaaataca tgagcagaaa ggatactgct gagagaatgg cgataatcgt
     3721 cattttgcgt gtgtttgtca taaggttcct ccaatttttg aaaattgaag aagcgtggaa
     3781 gagcaggcaa aaagcctcca cttcgtcttc tcccatccag actttactgt cggttgtgga
     3841 attgcaccac atcagctttc gctcgcagac taccaacggt ctattttgtt ctaatactgc
     3901 gttagctcat ctcgacatac attcagtatg gtcttcgatt cgctgcctag tcttaaaaca
     3961 aattaccacg ttttacactg ccggtcggga atttcaccct gccctgaaga ctatttcatc
     4021 ataccaaaaa agccttgcat ttgcaagagc ttggtaagtt ttcactattt taacttgtcc
     4081 ccatcatagg tgtgaaccaa atctagacca gcgaactgtt gctgccgaag agcttcatat
     4141 acaatcatac acgctgtatt agagacatta agactacgca catgttcatc attcattgga
     4201 atacggatag ccttctctgg atgttcacgc ataaaatctt ctggcaagcc cttatcttca
     4261 cgaccaaata agaaataatg aatgttccca tcattatagt cttcatccga ataggtttta
     4321 tcagcaaatt tagatactaa gtgaacacgt ccttgtttgc tggcataatc cataaattct
     4381 gctaaattgt catagaagcg aacatctaac ttatcccaat aatctagtcc tgcacgtttc
     4441 atcttacggt catcaattgg aaatcccata ggcttaatga tatggagagg actattggtt
     4501 gcagcacaag ttcgagcaat attgcctgta ttctgtggaa tctgtggttc aaataagaca
     4561 atgtggtttt tggctgtttc ttctatatac tctaatgttt caatattcat aaggacccct
     4621 ctggtgacat ttcactaaac acgaataccc ttcgaaaatc aaatccaaac catgtcagat
     4681 gcattttacc gtataccagt acggtctgtt gcttgttatc tagtttcttt ttttgatttt
     4741 cattgagtaa aaagactcga ctgtctccta tggcaagaaa gacaaaaaaa tagccacact
     4801 gcccggagtc aagctcagca aacagcatgg tgaatgatga ttcattcact taactcacaa
     4861 caggtttgag ttaaatcaat gaaatcgtga tactattgta gcttaaagaa ggctgaaaat
     4921 caagagtttt gccctatttt tttcaaattt tcatagaaaa tcatgggggc tgggcaaaaa
     4981 gaccaaccta gcttcgcaga gttcgtgtca acatctgagc gcagtgattg attggcttta
     5041 acagtccagt ggactgttaa tattgaggcg aacttgcttc gccctatctc cagccttcaa
     5101 aggctcccaa accttttgag agagtactaa aggcaacata aaaacctata tttatacaga
     5161 ataaaaagta ggttgcccta cttctgctta tttcttttca aactaaacct gtccggcaca
     5221 ggatcttccc caccttccac aaagggatga cagcgaccaa ttcgagccaa ccccatcaaa
     5281 aagcccctga gtccatgttt ttgtaaggcc tcgatcatat aagccgaaca agttggacgg
     5341 tagcgacaag agggctgaaa aaggggggaa ataaatttct gatagaatcg gaccaagccg
     5401 attaaaagtc tagtcattat ttttttactg gcttagtaac agccagggta tgtagtttac
     5461 tgatgtcttt cttattgagc ttgcggtatt ctcctggacg taggccggtc aagtcaagat
     5521 taccaaagcg agtacgagac agtttatcta cctgcaagcc gacagcctca aacatcttct
     5581 taacctggtg gtttcgacct tcatggattg ttaattctac aactgatcgg ttcttaacag
     5641 ggtccacctt gataatctcg tacaccgcag gcttagtctt tttgccatca atgacaacac
     5701 cacgggtcaa gggacgcaaa acttccttgt tggcaactcc tttgacacgc gccaagtaaa
     5761 ccttatcaat ctcattacgt gggtgaatca tttcgtctgt aaaatcaccg tcattggtca
     5821 aaatcaagac cccagatgta tcccagtcca gacgtccaac tggataaatc cgctccttaa
     5881 ctgttggcaa caagtccaca actgtctttc ttcccttgtc gtctgacaca ctagaaataa
     5941 cacctcgtgg tttattgagg agatagtaga ccttctcttc gttataaatt ggttttccct
     6001 caacctctac cttatcaccc gatttgataa ctgtccctag ctcacgaaca acctgaccat
     6061 ttacagtaac tagtccttgt ttgattagtt cttctgcctt acgacgactg gcaactccag
     6121 catgggcaat gtatttatta attctcattt tctaactcat ttctttctac gaatagctct
     6181 gtttcttctt gaatcaaatc tatttcagac acatcaggca attcttctag actattaatt
     6241 cccatgtaat ccaggaaata atccgttgtc acatagagat ttggtctacc aagaacctct
     6301 ttcttaccgt tctcacgaat gaggtcaaag gcctgtaatt tactgatggc accgctggaa
     6361 ttgactccgc gaatatcatc aacttcaatc cgagtaatgg gttgcttata ggctacgatt
     6421 gataaggttt ccaaaagagc ccgtgacata gtctgattga ttggtgtttt ggcatagatt
     6481 cgcaaaaggt ttgcatattc ttttttagta accaacttgt agcgattgga agactctaag
     6541 atggctaagc ctgacgcttt gtctgccata tatttctcac taagtttctc taactgctgg
     6601 ctgacagctg tcggctgcat ttccaacatc tctgccaaat ttcgtaacgt caagccatcc
     6661 tcacctgcca caaagagcag gacttcgatt tcagctaagc gattcatctt cactccttac
     6721 tagataaata tctccaaaag tctctgtctg ctccaagacg atttcatgaa ccttgaccaa
     6781 ctctaaagtc gcaaggaaga tcgtaatcat ttcattgaca tcttgacttt cttgaaaaag
     6841 ctgacggagt tcaaggcgtg gacctgtctc gaaacgactg cgaacaaagt ccatcatgtc
     6901 ttcgatttta tattcatcac gggcaatggt cgcatgagac tgacggaggc ttgcctgttt
     6961 ttcagccatt actttagaaa aagctaggaa aatgtcaatg atggtcttat ccttagctaa
     7021 ctgtacatca tcataaatca aatctaattt gggttttgaa aaataattgg cccgttcatc
     7081 atgttgctcc cctaatttct cactgagaag cttgaacttg cggtattctt ccaactggtc
     7141 caagaggtcc atttcaggat catcttcggg atcaatttgc tcaacaacct tcggtaataa
     7201 ccgacgactt ttgatgacca tcaattgact agccatgagc atatattctc ccgctacctc
     7261 taaacgcatg gcttgcaagg tagcaatgta ggccaaatat tgctcaataa cctctgtaat
     7321 cggaacctcg taaatatcta cttgatactt ggacaccagg tgtagcagga gatcaagtgg
     7381 cccttcaaaa tcttttaatt ttatatccat tatctaaatt tttccaaact catgcttgtt
     7441 ttcagtccca actgcttggc gatttggtcc aaggattttc cttcgtccat atgttttaga
     7501 atatactgct cccgtaattt ttgggctgtc caatcaggct tgccgatgga ctggacaaat
     7561 tctgtcagac gattaaaaaa ccattgacga gaataggtct gtcccttctt atcgaagaca
     7621 tagcgtccag acagatgaga ctccatatag ggtatcaatt ccttgggcag agtgagcacc
     7681 cgcttggctc ctcccttctc aactgtcagg acttgaaaat ccagattgac ctgctgacta
     7741 gtcagctctg ctatttcact aggagtcagc cctagtaagg caatcaaaag agtaatcagt
     7801 tgcccatcaa gccaaggact ttcttgaaag aggagagtta aatcctcacg ctccagtttc
     7861 tttttgacgc ttgctggttc tgacatcgtt tgcaatttat aaaaacgatc caggagatta
     7921 ttttcataaa gaaagtagag aaattgattg accgctgaca tttttctttt ctgagcagca
     7981 ggtttcaagt ctagaagaga ctgctgatag accaaaagag actgttggct aatctccccc
     8041 tttgttactg tcacaaactg ctgcaggtca taggtatagg acttttggct gttgacactg
     8101 acctttttgc tctggataaa agattcaatc gcctgcttca tttcttgcct tcttttgatg
     8161 ccaagggaaa attatgcaaa agcgcattga tggctttcag aatagattta cgggtaataa
     8221 tccccagaaa ttccctgttt tctccaagaa ctggtaggaa ggattggtcg atgagcttgc
     8281 gcatgacttc tgtcaaatca taatccagac ctaccgtttc ttcatctgtc tttacaatca
     8341 cagaaatatc tctgtttaac tcatcatccg atagttcatt ttcagcttga tatttaatga
     8401 tttctgtcaa gccgatggtt ccaaaaaagc ggccatcttc tgttaccaca ggtacgcgtg
     8461 aataggtcat gtgactgagt aacagttttg catggtcaat attatgcgta tcaataatta
     8521 ctgccaattt atccgctggt gtcaagaaag ttccttcctg gtccaataaa aagacttcaa
     8581 attctcttgc aatcatcttg aaatatcctt tgtcaatgct gggtaaagtt ggtgctgtcg
     8641 attgtagtaa tccacatgga agtgatcatc atagatggtc acaagagcat agagacactc
     8701 tctgaccaaa ccgcgaggtt gactaacgga gccaggattg aggaagaggg tcttgccacg
     8761 cacttcggca tcaggcacat gcagatgccc atacaaacaa atatccgcat caacttcctg
     8821 tgcccaataa tccaaccgct gccagccgta attaatgcca taaagatgac catgagtctg
     8881 agcgatagtc acatcaccca attgagtaat gagctggtct ggataaccgc caaaatagtc
     8941 gcagttgcca tttaccactt gaataccatc ccagagacta tcttgactat ccagctccga
     9001 atcaccattg tgaaagatgg catcgacctt gccaagataa tgatgtttaa tatcctctac
     9061 aatctgtcta tccccatgag agtcgctcat gactaggatt gttttgcttg ctcctgccat
     9121 actggaaatg cctccactaa tttttctagt gcttgtgcac ggtgggaaat cttatttttt
     9181 tcttctaggg ttaattctgc cgaggtctta cctgtttcac ctacgaggaa aagtggatca
     9241 tatccaaacc catcttcccc acggaggtcc ataccgataa agccttccca gtccgcttcg
     9301 acaaccaaac tatcacgatt tggagcagcc atgaccagag tacaatggaa ttgtgccgaa
     9361 cggtctttca gttcaaatac catggccaat tcgtgcaaga gcttggcatt attgagctca
     9421 tctgtggcat ctgggcctga aaaacgggca gaccaaactc ctggcaaacc acctaaagca
     9481 tcaaccttca aaccagaatc atctgccaag accatcttgc ctgtcagctc tgctatggtt
     9541 tcagccttga gccgtgcatt ttcctcaaaa gtcataccag tttctgctac atctggcagg
     9601 tcaggatagt tgttgaggtt ctcaacctgg tagccaaaac gctcaaaaaa cttacggaat
     9661 tccttggtct taccttcatt ttttgtagcg atcaagatgg tatctcctac gcttgccaat
     9721 cccttgtctt gaccgaagaa atctgccgtg ctaacccctg cttggggcaa atggactaag
     9781 agaagtttgt cattctcagt taccagaata gctccttggt gttggagaac cttgaactga
     9841 cgattgttta actcattcaa agtatcacag gcaaattgaa agagggcaaa ttcagactga
     9901 agtttaatca cttcaatgtt gacacactgg ttaaagttct cgtcttcata attgccagca
     9961 atcagtttgt caaataattg accaatctgg taaatttcct gctctctgcc attttcacca
    10021 aatgaaccaa atagattggc aaagctggct tttccgataa accaattgtg ttcatctctg
    10081 tattcgtata tcttgtctgt catagttcta catgctccac atgaatatca atgcccatcc
    10141 agctttcagc tatttcttta aaagctgccg gactagctgt tgtgtaaaat ctgtgctgaa
    10201 tatccttttc ggtacgactg cggttgattt gaaaataatt taataaaaca gaaatatctc
    10261 gcgcgcactc tgccccactg tcaatcaact tgacatcctt gcccatggca ttctggatga
    10321 tggggcgaag taggggataa tgggtacatc ccaagaccaa ggtatccact ttcccaacga
    10381 gtggtcgaag tgtctcataa acaacctttt tggcaagact ggattggtgg ctattggact
    10441 caaccagtgg tgcaaatttg ggacaggcta ggctgtctac ctgcgtttct ggagaaaggg
    10501 cctgaatctt ttgtcgataa atatctgatt gaatggtcat agccgttcct aagacaccaa
    10561 ccttaccagt ttgcgtcgcc ttaatagctg ccgaagcacc tggtaaaata actcccaaaa
    10621 caggaatatc caacttttcc ttgacttctt cccaggctac cgctgttgcc gtattgcagg
    10681 caaagacaat catttttaca ttttttgtca aaaggaaatt gaccaactgc caagtatatt
    10741 ctctgatttg ttccgctgga cgggggccat aaggtgcccg tgccgagtcc cctatataaa
    10801 caatttcttc atggggaagt tggcgcatta actcacgcgc aaccgttaat ccccccacac
    10861 ctgaatctaa aaaacctatt ggtcgattat ccataatact gagaatagcc agaaaatctg
    10921 actagccttt cttttctact caaaaaatgg gatctgggaa tgaatatccc agtcccagtt
    10981 tgattatttt ttcttgcttg ccactttttg ttggctcttg atttggcgaa gtacttgttg
    11041 tacttttgcc tcgcttggtt tttgccccat ttggctcatc atcaaacgca aagcattttc
    11101 atccaaaact ggcttgtcag caatgtagtt ttccacttgt ttgcgtgaca agtaaatacc
    11161 caaggctaca ccacctgcaa atgccaatac aataagtaaa attgctaaac ctaagttcat
    11221 ttgtcaatct cctgactctt ctaaaaataa ttcattcgga attgccctgc gacaatctct
    11281 taatgaatct attataccaa atttagcagg cttttccaag acttacctgt caaaatcaaa
    11341 accgtaaatg gtcgcgaaat aatcctctgg tgtttcagca cgacgaatca tacgcgctgt
    11401 gccatcttcc tgcaaaagga tttcagagga acgcagacga ccgttgtagt tgtagcccat
    11461 ggagaagccg tgggcaccac tgtcatgaat gaccaaggtg tctcctactt ctgcgcgtgg
    11521 taattcccga ttaaccgcaa acttgtcgtt gttttcacag agggaacctg ccacatccac
    11581 aacttcgatt ggcgcatctg ggcgcgtgat attggtgata tggtggtagg cgccatagaa
    11641 ggctggacgc ataaggtttg cagccgaagc atccacacct atataagtcc gataggtttc
    11701 cttacgatgg agcacctttg taatcaagtg accgtgtggc gccaacataa agcgacccaa
    11761 ttctgtaaag attttaatat gtcccatacc agctggcgtg agaatttcct cataaacctt
    11821 acggacaccc tcaccaatga cagcaatgtc gtttggttct tgctcaggac gataattgac
    11881 tccaatccca cctgataggt cgatgaagtc caaggtcaca tctgtttcct cacgaatttc
    11941 caaggccaat tcaaaaagtt gatgagctaa gacaggatag tagtcatttg ttactgtatt
    12001 cgaagctaga aaggcatgaa taccaaattc cttgacacca agctctttta gttccttata
    12061 gcctttcatc aactgttcct tggtcatccc aaacttagac tcttctgggt ggtccataat
    12121 atccgttccc agcgagaaga ccccacctgg attgtaacgg aggcagaccg tttctgggat
    12181 accagcaaca tttttcaaaa actcaatatg ctcgtaggca tcaaggttaa tggtcgcccc
    12241 aactttacga gcatagacaa actcttgcgc ttgggtgtca tttgatgtga acatgatgtc
    12301 cttaaagccc aatttttcac tcatgagtac ttctacatcc gtcgcacagt ccactccaca
    12361 accttcttct tgtaaaattt tcaaaatagc gggtgtcggt gtcgccttga ccgcaaaata
    12421 ttccttaaag cctttgttcc atgcaaatgc tgcattgagg gcacgggcct tctcacgaat
    12481 gcctttttca tcatacaaat gaaaaggagt cggaaattgc tccgtaatgg tttctaaaac
    12541 ttctttacta acaaatggtg tcttggtcat gtcactacct catcaaatat ttttcctatt
    12601 atatcacaaa tatgtgagga actgtagctt tcaactccac caaatacttg caaaataaat
    12661 tatgagcgct ataatgtgat tatcattaaa aaaggagtaa tatatgtcaa cattagattt
    12721 tgattttaca gaacgcttgt atgccaacta tctagctaat ccaagcctac aggcaaccga
    12781 aaatgccgtt agccacaacg gacttttgaa atccttagaa acacgccaaa gcgccatcga
    12841 caatgactat gtcttttcaa ttgacttgac caaggacgct gtttcaaacc aaaaggcttc
    12901 tggacgttgc tggatgtttg ctgcattaaa caccttccgc cacaagctca tctctgattt
    12961 caaactggag aattttgagc tatcacaggc ccataccttc ttttgggata aatatgaaaa
    13021 atccaactgg ttcctcgaac aaatcattgc cacagctgac caagaaattg gtagccgtaa
    13081 ggtaaaattc ttattggata ccccgcaaca agatggcggt caatgggata tggttgttgc
    13141 cctatttgaa aaatatggtg tggtaccgaa atccgtctat ccagaatcta tctcatccag
    13201 tgccagccgt gagctcaatc agtaccttaa caaattgctc cgtcaagatg cacaaatctt
    13261 gcgagacctc ctcgccaaag gagcttcctc gaaagaagtt caaatacaaa aagaaaactt
    13321 gcttcaagaa atctttaact tcctagccgt caaccttgga ctccctccac gcagctttga
    13381 ttttgcatat agagataaag acaatgtcta tcaccgcaat acaaatgtga cacctcaggc
    13441 attctacgag aaatacgttg gtttgaaact atctgactat gtctccatta tcaatgctcc
    13501 aactactgat aaaccttaca ataaatctta tacagtcgaa ttgttgggga acgttgttgg
    13561 tgcccctgct gtccgctacc tcaatgtaga aatgaaccgt tttaaagaac tagctattgc
    13621 tcaactcaaa gctggtgagt ccgtttggtt tggttccgat gtcggtcaaa gtagcaatcg
    13681 tcaaactggt attatggcaa ccaacaccta cgacttctct tcaggtctgg gtatccattt
    13741 ccatcaagac aaggctggaa gattagacta ctccgaaagc ttgatgaccc acgctatggt
    13801 cttaactggt gttgatttag acgataatga gcaacctctc aaatggaaag tagaaaactc
    13861 ttggggtgac aaggttgggg acaagggata cttcgtagca tcagatagct ggatggacga
    13921 gtacacttat cagattgttg tccgaaaaga attcctcaca caagaagaac tagcagctta
    13981 tcaagctcaa ccacaagtcc ttgccccatg ggatccaatg ggagctctag cataacaaac
    14041 gacatataaa aaaagagggc acccctcttt tttatatgtc ataattagtc tcgactcaat
    14101 ccaccaaaga tacggagtaa gttgaggaaa aggtttataa agtcaagata gagctgaagc
    14161 gccatagata cgacccaacc ttgaccaaca ttaccaccag tttgttcgaa aacattgcgg
    14221 atacgttggt tgtcataagc aatcaaacct gagaatacca aaacagaaat gatagacatc
    14281 ataaagctta gaccagagct acgaaggaag atattaataa cacttgcaat aataattccc
    14341 catagagctg cacgcaatgc ctgagccata ccagaaagat ccttcttcgt tgtcatccca
    14401 attacagcca tgatagcaaa cataagagcc gctgacacaa aggcaagaac aactgtttcg
    14461 ctggtataga acatcaagat catgctgatg gtaatcccat ttgttactga gtaggcaaga
    14521 aacattggta gagccattgg gctattttta gctgccattg ctgaagcaga aacaaccaag
    14581 gcaatctgac caatcataat cagcatcatg atgatggagc ctgcactcag cagactaagc
    14641 aataaagatt gaaagactgt aacagctagg aatgatacta atgctgatag tccaattccc
    14701 atagcaacca ctccgtaaat cttgccaaag aaacgattga gggcggtgtt atccacctga
    14761 ttaataataa aagaatcgtt attcatgata ttctccttta aatcatctta ttgaaaacga
    14821 aagaccttgc gatttttcca cttgtgttgc ataggcagac tccattctct tactgcccaa
    14881 tacttgtcaa cacaagtcac aatccaagac tctttatagc gaggaggagc aatcgttgca
    14941 ccaaacatgt gcttcacaat aatatcttct tctaatttat taagttgaat gagtttctga
    15001 gcattgcgct tagcaatccg tggatgaacc caagcatgac ttttattaaa cttagtatct
    15061 ctccagtcat agaaaaagag atcgtgtaat agcccaccgc gagcggtcga ttttgcatcc
    15121 catccgaact ttttcgcaat tttatagctt gtataagata cgttaatgga atgttccaac
    15181 cgtgtggagt aataatgatg aggaatcttc cccaattttt gaactttagg tgtagcaatt
    15241 aaatgcccca catagtccat gtattcttta tcttgtttat atgcaaccat tctttgaacc
    15301 tcgttggatt attataccac actatagtac aattggctta aataaagcta aatagtaaaa
    15361 ttcctgctgc aatcgccaca ttgagacttt cagcctgtcc tggcatggtg atgtgaacaa
    15421 gttgatccgc ctcatcagcc actaaatcag aaattccctg accttcgttt cccatgagca
    15481 aggcaaaact agaatgtggc gtaatttcct tatagtcaac cgactgacta gatagggtcg
    15541 ttgccaagat ttttatctga ttgcttttaa gagctgtaag aatggaggaa attggcatcc
    15601 gataaactgg caaatggaag tgactcccct gcatggaacg aagcactttc atattataaa
    15661 tatcagccga cttatccgat agaaatactc catcaaaacc agctgcatca gctgtacgaa
    15721 tcattgtccc aacatttcca gggtcctgca catcttccag aaccaaaaat tttccatcca
    15781 aagtctcagg tagaagttgg ctcggcaaag caagttgagc tactaccccc tgaggtgtct
    15841 tagaatcagc taaatcttgc ataatttcag gactgacaac tgtcacattg gctaaacctg
    15901 caaccttttc aaaatactct tccaccacaa aaatatgctc aatattggct ccagcctcca
    15961 tcgcttcctc cagcaaatgc cagccctcaa tcagataaga agatgtccga tattttttct
    16021 gttgtaattt tttaacttgt ttgaccaaat tgttggtctt tgagcgaatg atgtccatgt
    16081 ttgtcccttt tctttttctg ttcaacaact ttattaataa aataccgggt acgcttgata
    16141 ccaaatatat cttattccgt caagaaatca tcatcaagat ggtgaacagg attacccaac
    16201 catgatataa taaagtaaag gtcttgtcaa aaggagaaac ttatgcgtaa ggtaaaaatg
    16261 attgcatctg gtattgtcca aggagttggt tttcgatggt ctgtccaata ccttgctatg
    16321 gaaattggtg atatagctgg tagggtctgg aacaacgaag atggtactgt aaccatcctc
    16381 gcccaatcag aaaatgctga gaagctcagc cattttattc atgaaatacg aaaagggccc
    16441 tctcgtatgg ccaaagtgac ctacctagat gttaccctgg ccaattttga agactataag
    16501 gattttcagg ttagctatag ataaaacttg tatatttccg tcaaaaaaag taaaatagag
    16561 agtatactga aaaaaacaaa ggaacgaaaa aaattgaaaa ataaacgtct attcatgttg
    16621 tcaggcatgg ccttatcaac acttcttttc ttatctggtt gtgttcaaac tgataaaagc
    16681 ggaaatccta caggtctcat ttgggattgg ttgggacaac caatgtccaa cttgattcgc
    16741 ttctttgcag aagatcaagc tctaggattt ggtcttgcta ttatcatcgt caccttattg
    16801 gttcgattca tcatcctccc attgggaatt taccagtctt ggaaagctgc tatccaatct
    16861 gagaagatga attatctcaa accgatttta ggcccaatcc aggagcgcat gaaaaatgca
    16921 agctctcaag aagagcaact tgctgcacaa caagagtatt ttgcaactca aaaacaatac
    16981 ggtgtgagca tgctcggcgg aattggttgt ttgccgatgc tgattcaaat gccattcttc
    17041 tctgcaatct actttgcggc tcgccacaca ccaggaattt cagaagctac tttccttggt
    17101 attaatttag gttcaactag cctgattttg acagttgttg ctggtatttt atactatgcc
    17161 caatcgctct taatgcaagt cggcatggat gaagaacaga aaaaacaaat gaaaaccatg
    17221 gctctgatga acccaattat gatcatgatg ttctcatttg ggtctcctgc tggtgtgact
    17281 ctctactggg ttgtcggtgg tctcttcggt attcttcaac aagctattac aaacttcatt
    17341 ttgaaacctc gtattcgtaa acaagtggaa gaggaattca aaaatatcac agtgtcagca
    17401 actcctcgta aaacgaagga cgttactcca actgcttcag cagttattga agaaaagaca
    17461 tctaacaaga agagaaaccg taatgcagga aaacaacgtt ctagataata aacactaaaa
    17521 gattggaatt tccaatcttt tttagcacct tcgtgaataa aaagagagaa tctttcgact
    17581 ctctccacac cttatttcgt tttttcaacc ttcaagattt ttacatcata actaccagca
    17641 ggtgtttcaa ttgttgcaac atctcctgtt tttttaccaa tcaaagcatg accaattggg
    17701 ctttcatttg aaatcttgtt tgcaaaggca tctgcacctg cagcaccaac gatatgatag
    17761 acttcttcct cggtctcacc aacttcttga acagtcacag tcttaccaat tgctacttca
    17821 tccgcagcaa ctgcatcact gtttacgatt tcagcatagc gaatttttgt ttcgattgtt
    17881 gagatttgac cttcaacaaa agcctgttca tctttagctg cttcgtactc agagttttct
    17941 gaaaggtctc cgtaagaacg agcaatttta atacgttcaa caatttctgg tcgacgaact
    18001 agctttagtt cctctaattc tttttctagt ttttcctttt cttccaaggt cattggatat
    18061 gttttttctg ccatttgtct ttccttttct tttatttttc atgaaaacaa aattatgtgg
    18121 aaaaccacat aattttgttt taagaattta ctcattatta agatgtgcat ttacatactt
    18181 agcaacgttt tggttatgtt catcaatatt atttgtaaag taaacatttc cagttgtaac
    18241 atctgctaca aagtaaagat agtctgttgt gttggcatta atcactgcct cgatagccga
    18301 aaggcttgga ctatccactg gtccaggcat caatcctggt gtccaataaa tgttgtatgg
    18361 tgactcaatt gatgtatcaa tcgctgcatc ttctgctaga gttgtttctt gaccaagttt
    18421 accctgagca tacaagattg caatattaga ttgtagaggc attgctgcat tcaagcggtt
    18481 aaagaagaca cttgcaatgt ttcgacgatc ttcatcagta gaaccttctt tttcaaccaa
    18541 agatgccaag gtaagaactt catttacagt caaattctta gcagtaatcg tttcataata
    18601 tggttgtaaa cgattatcca tcgcggcaat catttgctca acgagctctt cgatagttgt
    18661 ttcatcgctg tattcataaa ccgctgggaa gaggtaacct tccaactgat aaataactcc
    18721 actatcagca gctggtaagc tggcaaacag ttttggataa gtagcaacca ttcgattaat
    18781 aaaatcttgg ttggtcacag tagccataaa ctgttctgca gtaaatggtg ttttatcgtt
    18841 cttatctttt gtattagtat tgtctgtaat agcctgagca atttgtgtta atgtataccc
    18901 ttcaacaatc aaaaccttcc ctgcagcttc tttttgagct gtcggagttc cgctttcttg
    18961 aagtgctttg gcgatgtcat ccacagacat attttgtttt aaattataaa aaccactttg
    19021 gaaattatta taactcttaa ttttagagta gtaattaaaa atagtagcat tcttaatcaa
    19081 cttgttgtca actaaaattt taccaatttc taaagttgaa gttccttctg gaatctctac
    19141 ctgaatagtt tctgtagctt ttgcatcgac tggttccaga ctagatttta cccacatata
    19201 accagtaaca cctgtcacta gaactgccac tacaacaatc gacatgataa cagagacaat
    19261 gtgtttagcg gcattatctt gttttttacg acgttgcttg cttgttctgt gggttgttct
    19321 tcttctgggt tgtgtttcct gcatctgctt gtctacttct ggaacaatag ccgttgtctt
    19381 tggagcaata atagtttcct ctagtgagat gatttctgta tcaccagtca cagctgacac
    19441 gtcttccgtt ggaaattcta aaaatgtatc ttctaaagga gaatttcccg gaaggacaga
    19501 ctctactggc tcagaaactg acgagcttga tggtacttca tcaaatcctg ccgtattagc
    19561 agccacaaga ttccttaatt tctccaagtt tcgttcaaca gtatcttgtg gaactggact
    19621 tgaaacaact tctttttcaa tcacaaaagt ctctggagta gccaaagttt ctgatgttgg
    19681 ctcttcaata ctttccgtca tcacttctga ttcttgctgt tcttctgaca agtatgaatc
    19741 agggaaagca ataccttcta cttttacaat ttcagtctct ctatgggggg ctttttcttc
    19801 ggcaagacta ggctggagct cgttgataac ttcattacgc ttatcaacaa gaacatccgc
    19861 tgactgttcc gccaaacgtt tcaccttcaa ttcttccaaa tctcgtaaaa tttgatcgcg
    19921 gaagcctgaa gactgcgtgt ttttttcatt cgtatccttt gtcacgctgt tactcctttc
    19981 acacagttac tatacattat atcttaaagg cttatgaatt gcaagaactt ccctttctac
    20041 aaggctttcc agtacatatc actccacctt tgtcctccaa attgcgacaa ggaggggcca
    20101 aaatcttcaa actggttcat ttcatagtag ctaatcaaat accccttaca ggtcagggaa
    20161 actccttcaa atcctcccgc taaagccact tcttttatgg ctgccaacaa aagagttccc
    20221 actccctgtc ccttatagga atccgctacg gatagactgg caatagctag gtgactgcct
    20281 gtcggcatgt cactctctgt caagtaaaaa atatcgtctg taacagtctg cgaaacagag
    20341 ggacaggata ataaatatcc tgctatttcc ccatcgtgtt ccattattaa acaggtttta
    20401 ctcaaagtat tcagataagt agcaagaaca gactcagcta tccgttcctc tttagaaaaa
    20461 ttggcatttt caatcaaaac aacttgttct aaatccgaag gagtagcaaa acgaatttcc
    20521 ataattttct cccttatata gcggaaaaac aggggccata ggcctccgtc tttcttttta
    20581 ttggacgtta tttgtaagat ttgacaagag tctttcgaag gaatattcat aggactgaat
    20641 atcaccagct cccataaata catatactgc attgtcatgg tctaaaagtg gtgaagtgtt
    20701 ttcaactgta acaagtccac cttttttgtt gattttagcc gccaaatctt ctaccttaac
    20761 ctgaccattg tctgtttcac gcgctgatcc ataaatttga gcaaggtaaa ctgcatctgc
    20821 tccattcaaa gcatctgcaa actcatcgag aagcgcaatg gtacgagtga atgtatgtgg
    20881 ttggaagatg gctaccaact ccttgctagg atacttctga cgtgccgcat caatggttgc
    20941 aatgatttcc gttggatggt gagcaaagtc atcaatgata acagtatcat tgacaatttt
    21001 ctccgtaaag cgacgtttga cacctccaaa tgtcttcaag tgttctgcaa ccagttgcaa
    21061 atcaaatcca gcaatataga gattggcaat aacagctgtt gcattcatca cattatgctt
    21121 accaaaggtt ggaatctgga attctcccag ttcttcttca ccatggcgaa ctttgaattg
    21181 tgaaccactt gtagaaggct tcaagtcata ggcaacaaaa tcgttgtttt cctcaaaacc
    21241 atagtaataa attggagcat ttgccgtgat acgacgaagt tgctcatctt caccaaaaac
    21301 gaggagagct ttttttactt gcttcgcata atcattgaaa gcattgaaaa catcctctag
    21361 actagtgaaa taatctggat ggtcaaaatc aatattggta atgatgctgt attcaggatg
    21421 gtatggggca aagtgacgtt cgtattcatc ggattcgaac acaaaatact gagcatttgc
    21481 cgaaccgcga ccagtaccat caccaatcaa gaaggaagta tctgtgatat tgcgcataac
    21541 atgtgccaat aatcccgttg tagaggtctt accatgggca ccagcaacac cgaggctcgt
    21601 aaaacctttc ataaattcac ctagaaattc atggtaacgt ttgtaggtat agccctgggc
    21661 atcaacataa gcgatttcca cattgttatc tggacgaaaa gcattaccag caatcaattc
    21721 aacatctgct gtaatatttt tctcatcaaa cggtaaaatc tgaatcccag cttgttcaag
    21781 cccgcgttgt gtaaagtagt atttctctac atcactacct tgaaccttgt gccccatttg
    21841 atgcaacata agagcaagtg cgctcatacc tgatccctta attccgataa aatgataggt
    21901 ttttgtcatg ttattcctct tctatatgtg tcaaattcaa ttcttgtgcc acttgtcgat
    21961 ctcgttgttg tctgcgctca ggataattgt aaacctgact ttttcttaaa aagtcatacg
    22021 aatttttctt tataacctgc tcctgttctc tatcttcttt cttcaaagaa tagacagctg
    22081 gcatatcagc gagaatatag tctgtctgac gtaagttgtc cgccaaacgt gtcaaccgac
    22141 ctgccttaga ttcagttgtt gaaacgacag gaggtttcac cacaacttta gctggtgctg
    22201 gtttttcatc tcttaagtag gttgccgatc gttttttctt caagtcttct ctagcctgtt
    22261 cgcgagccaa ctgcccctgt gttttcactg gagaaggctt cgttttagtc agttgctgaa
    22321 ggcgttcctt tcgagaataa tggcttggct tagcttcaaa cagaggaggc aggagctcat
    22381 cttccgtttg tgctactggt tttgccgacg caactcgctg tgattcggta gaccagctaa
    22441 attccttttc ttgataaagc cccctgatat tgctaatcag gtcgctctca tcatataaac
    22501 tcataattgg attttctcca actagtacct catcatctgc gactagtgga aatctttttt
    22561 catgtctaat cataagtcat cctttcgaac ccttattata ccatacttag gccttatttc
    22621 ctaattctac gctgagtcaa ctgattccat atcactcaat ccctaaaatc tcacgaatat
    22681 cctcaaccgt caaattcgct cggctttcat tgccatcaag gactgttttg acaaggttct
    22741 ttttattttc ttgcaattct tgaatcttct cttcgattgt ccctctagta atcaggcgat
    22801 agcattcaac cttttcagtc tgccccatgc ggtgagcccg gctgatagcc tgtgcttcca
    22861 cggcaggatt ccaccaaagg tcgactagga tgactgtgtc tgcacctgtc aaattcaagc
    22921 caacgccacc tgctttcaag gaaatgagaa aggcatctcg acttcctgca ttgaaggcct
    22981 gtgtcatttc ttggcgttgg ttagcaggtg ttgaaccagt cagagtatag gatgtcatcc
    23041 caatttctac cagctgttct tctatttttt ccagcatatt tctgaattgg gagaatatca
    23101 agaccctgtg ttcaccttct tttagctgca agagtaattc tttcaaacta ttaagtttgc
    23161 cactctcacc actaaactct tccatgaaaa ggctgggggt atcgcagatt tgacgtaagc
    23221 gagtaattcc tgataggatt tctatcttgc tacgattgat ttgagcatca tctgcgccag
    23281 ctatttgagt ccgcatttgt tgaagctggg ctaggtagat ggctttttgt tcatctgtca
    23341 gttcattaag tacattgacc tctatcaaat caggcaattc ctgaagaacg tcttccttat
    23401 gacgtctgag aacgaagggt tgaatggtcc gcgcaatgtc cttggctgct aatttaccga
    23461 agtcttgttt gcctggcaat aaacctggta agacaatttg gaaaatggac cagagttccg
    23521 tcaaatggtt ttcaatcggc gtacctgaga gggcaaagca attcccaacc tcaaactcac
    23581 gcagtagctg tgcaatctta gattgggcat tcttcatgac ctgcgcctca tctaaaatga
    23641 ggtagtcaaa gcgttcctgt ttatagagtg caacatcctg tctgaaggat ggataggagg
    23701 taactaagac ttggtgacca ttggcaatga tctcttcacg ctgttccttg tttccatgga
    23761 caacagccac atctaaactt ggagcaaacc gcttacactc atctaaccag ttataaatca
    23821 agctagacgg tgccaatatt aaaactttgg aatcttttgt catccgactg gacagaaagg
    23881 cgatagtttg caaggtcttt ccaagcccca tgtcgtcagc taaaattccg ccaaaaccat
    23941 acgtatccag catagtcaac cacttaagcc ctgtctgctg ataatcccta agcgacgttg
    24001 ttacttccac agcaggaagc gggaacaaat ctggttgagc tagatagctt gccatttcct
    24061 caaattcctt ggagaaactg acttggttga attgtgacaa ggattgagcc aattggtaac
    24121 cggcaagagc atggacttgt acctgcccct ccttaccatg acgggcccgc aaatagatta
    24181 gggtttgact gattttcttg gtttcctcat caaaaacaaa taccttaccg ctcgggctag
    24241 tatagtggtc ctcttgattc agcaaggctg taatcgcctg atcaatttca gattgttcaa
    24301 tacctgataa gtcaaacgaa atatccaaaa gtgagccgtt acgcacaatt tctaattgag
    24361 gtttggcttc aatatataaa gcctgtagcc tttctgctag atgaacctgc cccatttttt
    24421 gtaatatagg caattgttgt tggaaaaatg gatagagctc ctgctgacta agagccgctc
    24481 tctgggcatg atatgtcccc ctaaagccag ctaaacgaat agcctgatag accctctcca
    24541 aatgttggaa atggctggca aaaggcagga gagccaaatc ctcttcactc cttaccttac
    24601 gtccatcaaa atccaaaacc aattggaggg ataaactttc atttgcctcc atctgaatat
    24661 gaaattctgg cttgaaatca tgaatgatga agcgtttagg tgccttaaca gtcccaattg
    24721 tctgcaaatc cagcaagctt aaagccagac gattttggtc agggaaatcc acctgcactt
    24781 ttctcaaacc tgattcggtt ggaacaagtt cggaaatttg gtaaatcaga tgttcttgat
    24841 ggcgattgac actataaacc gtgccattta ctaagagata gcgcccatga aaaagaggtc
    24901 ggactgtttt ttcgtgaata accatttcaa tcatctgcgt gtgaacggtc acctcaaaat
    24961 gatagagccc ttcctcccct gtcaacggaa gaaccttcaa ggaggaatag gactgctgtt
    25021 gataggaaaa gttaaaatgt tctagctgat ttaacagctc taacccctcc tcaaaatagg
    25081 cttgtggcag acgaaaatgt cgtccaaaat gagtaagaat atccgaatct ataccaactt
    25141 tctctggtac caaggcccag agaaaatcaa ggagagcctg acttggttca tcaaaatttt
    25201 cataagtaac ttgctcataa taatttttac ctatttgata gtgaccgttg gctttcacaa
    25261 tgcgcaaaaa agctccgatg tcccgcacaa tgtacgaacg tgtatctggc aaacgggtaa
    25321 ttttcaaggt ccaatctatc tgacgatcat agggcaataa gctcccttca actgacaact
    25381 gatacttaag ccccagttca ggttctggcc gcaaaatttc atctaaaaag agaccgccaa
    25441 aataagtccg acggacggtt tccttatgtt gttcgctatc ttctttcaaa gactgcttca
    25501 tatccttgcc tgaggcatca ttttttaaaa aatactccgt ggcagctagg tgttgacagt
    25561 agcccttact ttgaaataac tggcaactac actgtaaatc gctatctgca tcgccgtaag
    25621 taaagcgctc accagcaata tccagcacca tctttccatt ctcactatga agaacagtca
    25681 gttttccagc ctcgtacaaa tcaattcctt cttggcgaat ccgccctggc atcatgcgac
    25741 ccattctaac tcctttatcc ttcaaccaat catcaactct tctgtaaaat atatctaaaa
    25801 tattatcttt tcattatagc atattttcaa tttgaattgt ttaaataggt atcaaaaaaa
    25861 caccagcagg taaactggtg cctctaatga atagctaaat aataaatcgt tgcttgccac
    25921 attttcaaga ttgtttttcc gcttttccaa agccaaaata gggcaaatag caggaaaaca
    25981 actgccacta ctacgttaat caagcctttt ccaaccaaga aaaacttgaa aaagaagaca
    26041 gtcacacaag tgagcaaggc aaggagtaag acatgaatca gaactcccaa ccagcccagc
    26101 caatagcgaa ttccaaaggg agggtgtttc ttttcatcga agtctgctag accagtcaaa
    26161 aactcaacta gcaactcacc aaaaaattcc atactagcct acctttctta cctgtcgata
    26221 atatttggtt cgtcgaatca tcatcttgat agccttaaag gaaagtaagg ttagcatact
    26281 agccacaaat agacaaaaaa ggaatatcac aaccaatggc tgtttatact gattatagac
    26341 cataattgaa ccaccagtaa aggcgataaa ataggatgcc aaagtcacaa aattgagcca
    26401 gatacaagcc actagcccca gccaataacg aaatccgaaa ggcttatact gactgtcttc
    26461 ataaaataca agaaaaaatg atgaaaaaga aagcaatatc aaaaaagaac gccaaatcgg
    26521 aaccgctacc ataccaacac ctccaagtcc attctagcat atttacgaat aggtgcaaag
    26581 aaaaaagagg tttccctctt tacttccgtt tccgtgcaat cagatgaatc ggtgtccctt
    26641 caaatacaaa ggccttgcgg atttgatttt ccaagaaacg catataggag aagtgcatga
    26701 gctcttcttc gttgacaaag accacaaagg tcggtggttt ggtcgcaacc tgagtcgcat
    26761 agaaaatctt gagacgttta cccttgtcag ttggtgtcgg gttaatggca atggcatcca
    26821 tgataacatc gttgagaaca gccgatggaa tacgggtatt ctggctttca ctgatggcct
    26881 taatcatctc aggcaacttg tgcaagcgtt gcttggtcaa ggctgataca aagataatcg
    26941 gtgcgtaaga caagtattgg aactggtcgc ggatatcatc ttcccatttc ttcatggtgt
    27001 ggttgtcttt ttccaacgtg tcccacttgt tgaccacgat aatcattccc ttaccagctt
    27061 catgggcaaa acctgcgatg cgcttgtcat actcacgaat accttcttct gcattgatga
    27121 ccatgagtat aacatccgag cgttcaatgg cacgcatggc acgcataaca gagtatttct
    27181 ccgtattttc atagacctta ccagacttgc gcatccctgc ggtatcaatc atggtaaatt
    27241 cctgaccttc tgggtctgta aagtgcgtat caatggcatc acgagtcgtt ccagcaacag
    27301 gcgacgcaat cacgcgctct tcacccaaaa tagcgttaat caaacttgat tttccaacgt
    27361 ttggacgacc aatcaagctg aacttgataa tatctggatt ttcttctgct tcttggactg
    27421 gcagattttc aatgatagcg tccaagacat cccctgtacc gataccatgc acagatgata
    27481 ctgggtaggg atccccaaga ccaagcgaat agaaatcata gatgtcatta cgcatttcag
    27541 ggttatcgac cttgttgaca accaaaatga ctggcttatt ggtcttgtaa aggatacgcg
    27601 atacatactc gtcagcatcc gtcacacctt ccttgcctga tacgacaaag acgataacgt
    27661 ccgcttcatc catggcaatc tcagcctgat gcttgatttg ctccatgaaa ggtgcatcca
    27721 catcatcaat accgcctgta tcaatcaagg aaaacttacg gttgagccac tcaccagtag
    27781 cataaatacg gtcgcgggtc acaccttcca cgtcttctac gatagaaata cgttcccccg
    27841 caatacggtt aaatagtgtt gatttaccaa cgttgggacg gccaacgatg gcgatagttg
    27901 gtagagccat agctcctcct cttctagttt acaataattt attcttttct aagatagact
    27961 ttgtttctgg ttgttcagcc tgaccaaact gggcaaccat tcgctcagac caagtttctg
    28021 tctgacgggc acctgcatag gctgtctgaa cttggtcgta ggcttggatt acttccacgc
    28081 cttgctccac atattcttcc tgaaagacaa tggtttcact tgccaaacgt ggtttaacag
    28141 gatggttttg ggctggtcga cccaaggcaa tacagaaaat tggataggta taatctggca
    28201 agttgaacag ctctgctatt gccatggcct tatggcgaat cataccgata aagacgccac
    28261 cgtaacctag gctttcagct gctaaaaggg cattttgccc tgctaggctc gcatcaactg
    28321 aagaaatcag gagattctct gttcctttgg catcaaagtc tgaaccatgg agttgagccg
    28381 ccttgctggc acgattatgg tcgcccacaa aaaccagaat ggcctgagcc tgtaaaatag
    28441 ctggctgggg caccaaatca tagagtgcct gttttttctc ctccaactgc accacaatga
    28501 tggaataaga ctggaaattc ttccagctag acgcagctcg cccagctgaa ataatagctt
    28561 gcaaatgttc cgcctcaata ggctcctccg taaaacgacg gacggaagta tgattcagca
    28621 tcaagtctat ggtttcattc atcggcggtt atttcctttc aaatgcaaat cgcgagccag
    28681 atagcgaatc cgctccataa cccgtttggc ctgccaggtc tcatcaccag acttgccaga
    28741 ggcgaaatga cgctccaaat catcaaagga gaaattggag gtaaaaaagg tcggcagatt
    28801 ttcctgcatg cggtattgaa gaatgacttg caggacatcg tcacgtaccc aaggactatg
    28861 ttgctccgct ccgatgtcat ctagcaccaa gacctgagcc atcttgattt catcaattcg
    28921 ctccttgaca gaaccatctt tgatggcatt ttttatatcg acaacaaagg ttggataatg
    28981 aagcatagtg gttgattgaa gatgggtctt ggacaagaga ttggctaaat aagccatcaa
    29041 ataacttttt ccaataccga aattgccata gatgtagagc ccttttggtt tttcctcaaa
    29101 tcgtttgaca aaatctgcaa tagccagcat gacttccaca cgattttcat cactcttatc
    29161 aatatccgcc actgttacat tcttcaaact cgcaggcata ttgatgagct ggatacgatt
    29221 cttgatggct tccaaacgac ggtattcgac caattcctct gtctcaagat aggacacatc
    29281 cgcatagccc tcgttcatga ctaaaaccgg ttgatagcct tttgcaatat aggcttcatc
    29341 ctgcttgaca aagaggtcac gctgactgat atattccaaa aacttagaaa tggagagggt
    29401 gatttcctgc tgggtcagac cttctttttt gataaaagca gcgacttcag gatcactcac
    29461 tatctcctga tagagttgct gataagattt tggacttgga ttggttgttt gcgacaacct
    29521 gtcttgaact gatttcatca gctacctcct ttgttttcca tttcttctaa ttcacgataa
    29581 agagccgcca gcttggcttg accttcctgc gtttgtcctt gctggacttc ttccttactc
    29641 cactctggca cattggtttt ctgtggctgg ctcgacttgg taacaacttg acctgtcttc
    29701 aactctctta ggtaaagaac agctgcctct gcactgacac ttcccttgta ggaaaaatca
    29761 ttgcccagct tcatagcgta tttttcattt agattggctg agtccacctt gttaaaggta
    29821 tagaggacca agacattgat gacttcgtca agcagaccca aattggccaa atccgtcaag
    29881 cattttcgtt ctgttcctgt cacagctgcc ttgcgttgct ccttgataaa ggacaggaag
    29941 gttaggctag attttgattt cgcttcacga acaatggctt gttcttgggc agtgaggttg
    30001 gcgttttctg ttggcaatga ctgacgtgac tgttgcaagc gtttggtcga gatggtctgt
    30061 ccaatagctg ttgctttagc ctggcgatag gtttccaacc aggtccaccc cgcctgctct
    30121 gctatgtaac taagcgcaat aatatcctca gcttcatcct gaaaaagaag cttatcttta
    30181 gccattaatg ccttgaaggc agaccagtca aaatcatgtt tcggttcaaa aacaactgga
    30241 gcctgcccat ccatggtaaa gacctgagaa aatgtttttg agatattctg ttcttgattg
    30301 ggttggtgaa caagcagatt ctccatgctt gactccccaa tcttccttgc caacaagttc
    30361 ttatacaagg gtttcgctag aaaacttttt acggtcagag gggcttgtag cgccaaacct
    30421 gttaattcat ctgcacgata gagttctagg agccccatgg cagtcagaac atccagagcc
    30481 ttctccaaac gattcatccc aaaatccatg tgattgagaa tgacagtcca tttgtaacgt
    30541 ccttgccctt gatcggcaaa actataaaag taataataaa gcgctaatgc atcaaaaccg
    30601 ataatcggct ggtagcattg gctaagactg acaatatcag gtgtaaacgg gctgattttt
    30661 atataggcaa ataaatcatt tggtttcatc tttttttccc ggttttacct tacttccttt
    30721 ggaaatcatc tgtttaagca aattttctag ctctccaaca tctttgaaag aacgataaac
    30781 cgatgcaaaa cgcacatagg tgatttcatc caattcgacc aattcttcca ttaccaaatt
    30841 cccaatgaca tcggattcta tttcactatc accttgtgct cgaaccttct gttcaatccg
    30901 attcaccact tcatcaatat catcggttga cacagggcgc ttttgagcag aacgaataat
    30961 cccattgaaa atcttttcac gtgaaaactg ctctctagtt ccgtcttttt tcacaacgac
    31021 tagagttttc tcttcaatcc gttcataggt ggtaaaacgt tgaccacatt ggtcgcagga
    31081 acgacgacga cggatggtat tgccatcttc agcctgacga ctatcaatga cacttgattt
    31141 caaactctga cattttggac aacgcatact ctttctccta cataaacttt atcacttcag
    31201 tatagcataa aaatgacaaa aataaaagaa agcgagaatt actcactttc tgccaataat
    31261 tcaccttgct tcttcaatcg actggcaacc cattcacgaa ccaagcgata gacaaccgca
    31321 aaaattgggg taaagaagac cataccaagt aggccaaaca aattaccacc aatcaaggct
    31381 gctgctaggg taaagagagt cggaagacca actgattgac caactacgcg cgggtaaatc
    31441 acattccctt caatcaactg aataatctgg aaaagggcga tagaccagag tgccaagagc
    31501 ggattttgta ccgccacaaa aagagcacta atcaaacacg ctgaaaaggg accgatataa
    31561 ggtacaaatg ataggacccc agccaaaata ccagccatgc tggcataagg aattcctgag
    31621 agcgaataac taacaaagac aagtgtccca atgatacacg cctcaacaat ctgactcatc
    31681 aagaatcggt cataagtatc aacaataact tctccaatat agttcactac tctgacagcc
    31741 ttctctggca aggtagcctg caagaacttt ctcgtcatcg cctgcaagtg ttccttgctc
    31801 cctaaaatcg ctagagtgaa gaagaaagcc attatcaacg tcatagtgtt cgaaaaaatc
    31861 cccgtcacat tggacaccaa gccagataaa atcggtgtga ccaaacttga aataagtgtt
    31921 aaattcttca actgatccaa aaagctggca atctggtctc cgatttgtcc cgacaaaaga
    31981 ccatttgatt caagaaaatt agttagggca tttaccgatt ttggaatagc tgtactacta
    32041 accgaaacca gttctgaaac agtcgtcacc aatgtaggaa gaacaatcac caccagtcct
    32101 gtcacaatta aagcaaatcc aattaatact ccaacaattg ccaaagaccg cttggatttc
    32161 tttaaaaaag aaattttttc aaactgatct tctaattttt tcatgggaac attgaggatg
    32221 aaggcaaaaa tagctccaat aaaaagcgaa ttcatactac ctaataattg ttgaatagct
    32281 ccataaatac tagaccagtt gagaacagct aacaatgtcg ctccagataa gagtatcaat
    32341 atgacttttt ctttaaatga tgtattcata acttttccat tcttacttat tggctattat
    32401 tttatcacac gcttatagaa aaagaaagag ggaaagcttc cctcctctct actctaaatc
    32461 aatcgcgcat ggcatatccg acaccacgaa ctgtttttat atatgaatct tgatgtggca
    32521 aatccagttt accacgtaga tagcgaatat atacatcgac gacattggtc tcagaagccg
    32581 cttcatattt ccaaacacgc tcaagaagtt gttctcgact aacaggctct gggctattca
    32641 ttaaggtcgc caacagatca tactctcggc gggtcaaatt aatcaattca tcacctcgag
    32701 tcactgttcg attttgaaaa tcaaccttca aatcacgata ggccgcatgc atgtgaacct
    32761 gtttacagtt attgtcaata aaatcacgac cacgaaaaat cgcagagatt tgctccacta
    32821 aatcactgat gacaaagggc ttcactacgt aggaaacagc ataagagagg acttcttccc
    32881 catattgact aacttctgtc ggctcaacta ccacaatcat gaccgtggct ggttttatcg
    32941 ctaatagttc tgcagccaat tccttgctgg acatgtctga aagctgaaaa ctcatcagaa
    33001 tcaagtcaaa atccgtttca tgtgccaagg acatagcctc tttccctgtc gatgcataat
    33061 caacaagata gtcttttttc tgcaattcca tggaaacaaa atgcgagaga ttgcgttctt
    33121 taccagcaat caaaattttc ttagccattg tccatccttt tctattcttt caaatggtgt
    33181 aggaatcgta ttatgactag aaagaagggc gcttccacgc ccatcttttc tattttgatt
    33241 cgctatacca gtcgtaatgg aagattcctt ctttatcttt acggttataa gtgtgagcac
    33301 caaagtagtc acgttgtgct tgaatcaagt tcgctggcaa gttttcagca cggtagctat
    33361 cgaagtaggt aatagctgct gagaatgttg gtacaggaac tcctgcttga actgccaagg
    33421 caacaacatc acgaactgcc tgttgatact tagctgttac atcaaggaag tactcatcca
    33481 agagcaagtt tgccaagtct tcatcacgtc cgtaggcatc tgtgattttt tgcaagaagc
    33541 gagcgcggat gatacaacct gcacgccaga tttttgcgat ttcaccaaat ggcaagttcc
    33601 agttgttttc ttttgatgct acacgcaact gtgcaaaacc ttgtgcataa gacatgattt
    33661 ttgagaagta caatgcttga cggatttttt ctaccaattc agccttgtcg ccttcgtatg
    33721 caaacggagc tggttttgga aggaccttgc ttgctgctac acgctcatct ttgtaggttg
    33781 agatataacg agcaaataca gattctgtaa tcaatgacaa tggcacaccc aagtcaagtg
    33841 atgattggct tgtccattta ccagttccct tgttaccagc cgcatccaaa atgtagtcaa
    33901 cgattggtcc atcttgacct tgatcgtctt tacgtttcaa aatatctgct gtgatttcaa
    33961 tcaagtagct atccaattcg cctttgttcc actcagtaaa gatgtcagcc atttcatcca
    34021 ctgacaaacc gagcaagtgt tgcatgaggt cataagactc cgcaatcaat tgcatgtcgc
    34081 catactcgat accgttgtgt accattttta cgtagtgacc agctccatct ggaccgatat
    34141 aagtcacaca tggtgctcca tcttctggtg cttttgctga aatttcttcc aagacatctg
    34201 ccaccaattc gtaagcttct ttttgaccac caggcatgat agatggacct tcaagggctc
    34261 ctttttcacc accagataca cctgtaccga tgaagttaat acctgagttt gccaattctt
    34321 ttgaacgacg gatagtgtct tcgtagaaag tattaccacc atcgatcaag atgtcacctt
    34381 catccaagtg tggcaagagc gcttgaatgg ttgcatctgt accaggacca gcttgaacca
    34441 taagcatgat gcgacgtggt ttttcgatag atgccacaaa gctttccaca tcgtaacttg
    34501 gtaccaagtt tttacctggg ttgcttgcaa caacgtcttc tgttttatca gcagagcggt
    34561 tgtaaatagc tactgagtag ccacgtgatt caacgttaag tgcaaggttg cggcccataa
    34621 cggccatccc tacaacacca aaatttgctt tagtcatttg tttactcctc taatttgtat
    34681 ctcttttatt atacaccgaa agctgaaaat tgacaagaat tttcagataa tttaactatc
    34741 ttgccaatct gagttagtcc tcatctccaa ataacccttg taattgggca aaaggactat
    34801 tggcctcttt tttctcttga gctttttgtt gaaaatcttc ttcagtcatt aaagcccaag
    34861 cttgaccaga tggtagttcc tgtccagctt cttcttcagg tgtcaagact ttgattggta
    34921 tagccagtaa aatattatct gctacactct catcaaggac aatgtggtca tcttctacta
    34981 ccagtaccat atcctcatca atcaaatctt gttctttaag aactgcttca ttggcaacaa
    35041 agagttcatt gacgttttgg acctcatgca aaacaactgg ttgcagagaa cgactggatg
    35101 ccaaggtaat atcataagtc atctgatagt ccaagaaaaa gaaacctgat tcaaaacgga
    35161 tatttccagt gacttcgact ggcgacaagc ctagaacttc accatttcga gcttgcaatt
    35221 cttcctgtaa atccaaggtt ttatcaaagg agatgccatc tagatttttc tgaatatcgt
    35281 aaatatgaaa cattgtattc ccctattcag ctagttctgt gatgtagtta tagacttctt
    35341 gtccagcaac tgcaccattt cctactgccg ttgcaatctg acgaagttga ttttgacgaa
    35401 tatcaccgat ggcatagatg cctgattgag tcgtctccat cttttcatta gtgatgaccc
    35461 aaccagcttc gtctgtaatt cctaggtccg caactgtatc agtcatcgga tccagaccga
    35521 catagataaa gacaccaccg aaatcaagtt cactgacttc ttcagttttc acatttttga
    35581 taaccacgct ttgtacacgt aagtcatcgc ccttgatttc ttcgactacg ctatcccaga
    35641 taaagtttat tttttcattg gcaaaggcgc gatcttggat gactttttga gcacgaagtt
    35701 gatcacggcg gtgaacaatt gtcacagact ccgcaaattg tgtcaagaag agagcttctt
    35761 cgaccgcaga atctccacca ccgacaacca agagtttttg tccacggaag aaggcaccgt
    35821 cacagactgc acaatatgag acaccacggc tattgtaagt atcctcacct ggaataccta
    35881 acaaacggtg ttttgccccc atggctagaa cgactgtctt tgtctccaaa acaccatcct
    35941 cagtaatcac tttcttaatt tgtccttctt cctcaatacg gactagggtg ccaaaaatat
    36001 ggtccacacc gaatttttcc aagggttcaa acattttttc agccaaggct ggacctgaaa
    36061 tatggtcata gcctggatag ttttcaattt ccgcggtatt gttcatctga ccaccataaa
    36121 taccacgttc caaaagagct acctttaaat tgctacgccc tgcataaaga gctgcagtca
    36181 tccccgcagg acccgcacca attacaactg tatcgtacat atactttttc cttctacgct
    36241 ttaaacatta ataagattcc cactactcca aaggctgcaa tgggaattgt catcacatta
    36301 atcatcaaca aatcaaacaa gaaatcttgt cgttcttgca aggttttatc cacacccttt
    36361 gtcgcacgcc agataaacca gaccagacta gaagtgataa tcaacaagga agctattagc
    36421 aatccttgta aataaaaact tagaatgttt tgtaacatct ttttcctcga ttactttaaa
    36481 aggtttgacg cttggcttta cgttctgcac gaccctttgc acgcgcttca actcgcttgg
    36541 tcttgcgacg tttttcatca acagcccatt gaattttctt cttgtagcct ggtttgactt
    36601 ttttcttttt cttcttgacc aagccaatca tttccaaatc tagtttttca cgagattttt
    36661 cacggttagc acggcggtca cggtcgtagg tatcgacaaa ttcgccattt ttcatttctt
    36721 ttggcaagaa tttgatattg agtttttcta gctctcgaat atctgcatca tcgcttggct
    36781 gataaagggt aatggcaatg cctgataaac cattacgacc tgtgcgcccc acacggtgaa
    36841 caaagaagga aagatcctgt ggaatggcat cattgatgac atgactgacg ccctcaatat
    36901 caatcccacg tgctgctaaa tccgttgcaa caatgtactg atagtccaag ttcttcacct
    36961 ggttcatcat ccgcttgcgc tcacgtggag gaatatcccc atgaatcttt gcgacacgaa
    37021 gaccgttcgc agtcaaatag ctatgcaatt catctgcacg tgtctttgta ttgacaaaaa
    37081 tcatggccaa gtaaggttgc atgagcttgg ttacttccaa gatttgagca ttacggtcac
    37141 gccccttggt tgaaatcagc cagttttcaa tggtatctga aatgaccgta tgggtcttga
    37201 tttgttccat gactggattg gacaggtatt ttttcaaaaa cggttggagt ttctgcggaa
    37261 tggtcgctga aaagaccata aattgcaagt cttttggcag gctgccagca atcttatcaa
    37321 ctgtttccaa aaagcccatg tccaaggtca tatctgcctc atcaaccaca aaggttttgg
    37381 ctttatggat agcaaggtca cctgatttga ccaaatcata gatacgccct ggtgtcccaa
    37441 caacaatgtg aggctgacta gcttgaagct tgtcaatttg acggttctta tccgtaccgc
    37501 caacataatt ggtcacacga acttcgatgt ccgaatgact tgccaactgg cgtgccgctt
    37561 gataaatctg agtagctaat tcacgagatg gggctgtaat aacagcctga acttggtcca
    37621 attcctcact taatttttgg aaaataggga tcaagaaggt atgggtctta cccgaacctg
    37681 tttttgattc ccctatcaag tctcgccctg acaaaacaac aggaatcaat ttctcctgaa
    37741 cttctgtcgg ctggacaaag ttaatttctt tcaaagcctc ctgaatataa ggcttgagtt
    37801 tcatatctgt aaatttcata gtttcctctt tagttgtatt tccctctatt atagcacgat
    37861 ttaggtggac aagcaaaaag gacggatacc cgtcctaatc aactctcgct tgcttcagta
    37921 aattctgcct caataattcc aagttccttc tcttcttcca aacttgcttt aaaggcctca
    37981 gtgagttcta attcgccatc aaaatcacca tttatttttt tcacaaatgt atctgccaac
    38041 atccctccca tgggctggaa ggtaaaatag gtcaaacctc ctgaaatcag accacccaaa
    38101 ataggaactg cattggcaat ggtcttttca acagtctttt tggtaatttt cacaccaatc
    38161 actgcggcta cctttttcac caagggatgg gagc
//
LOCUS       DAZQCJ010000007        37357 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000007 DAZQCJ010000000
VERSION     DAZQCJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37357
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.007"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            624..2351
                     /locus_tag="ACHU66_000361"
     CDS             624..2351
                     /locus_tag="ACHU66_000361"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3388676.1"
                     /translation="MFRLIFDYIKRHKWLYLLVAVTLIIYDATLLLPTQIIQRMVDIL
                     TKNELTQAILVQEMTLLLLVTVLNYATAFIWHLKLFQASVNFKFDMQQRAFKKLVTMR
                     TPFYEKFRSGDVMTRFSTDVDGLMEMVGYGLMIVVYAGGMLAFIIPTMFFIDWKISLV
                     ALLPMLFMTLCIFFIGRKQDKAIDANREAVAQLNNEVLEVIEGIRVTRAYSKKATQKA
                     QFQARTKQLAQGGDRITSLQSLYNPLATVCLGLSTIFVLLMGAQAVKAGQLTLGQVIA
                     LQLYVGSLLEPFWTLADFILVYQTGKTSFEKLQELIETGDDLEADGSKEIAELSSISF
                     KNYSFSYPQAERASLQDINWTLKAGQTVGIVGKTGSGKTTLVRQFLRQYPIGQGNFFI
                     NHQSVLDFKRSSIEEKIGYVPQEHILFSRSVGENIALGKVASSSEEIEQAIATAAFSQ
                     DLKRMTDGLDTMIGERGVSISGGQKQRISIARAFLREPDLLILDDSLSAVDARTERQI
                     IQNIQKERAGKTNVIVTHRLSAVNHADWVLVLDEGRIVEEGRPADLLAQRGWYYEQYQ
                     RQQSQEGGE"
     gene            2351..4090
                     /locus_tag="ACHU66_000362"
     CDS             2351..4090
                     /locus_tag="ACHU66_000362"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3388677.1"
                     /translation="MTILGFLLKQIRRVKWLFIVAVGFYLLASTMVRLAPLLIQQAID
                     GPITDLSKGLPFDEAVFLNQSAQYMGMIILGAIGFYLSMRLLMHCANRIAENLRNQAY
                     DVMQRLPISYFDDKPAGKIATRIVNDTETLRTQFYGTLVNAFNNIVRLLFTYGVLFYM
                     NRSLGWLMLLLIPLYIGIQFAYKKMTDKPMKDFYDARSDVNTQVNETMNGASLIQLFG
                     QEERIMKEFEVTADKMRRADNKIIWAQSLATWNLSGFLQNLVIAAILTVVGYQFLAGQ
                     YGVTAGKLFVYVNYIEGVFIALGALVQQFPNMLRSFETGKRLMALLEEEVEDDCDRIL
                     EVEQGQVVFEHVNFSYEENRPVLKDITIRAEKGETVALVGHTGSGKSSIMNLLYRFYD
                     PQEGRVLIDGKNIRDYSRESLRSHMGIVLQDPYLFTGTIASNVSMNEEEADRTRIMQA
                     LEKVGAGPMLSRLEKGIDEPVVEKGAAFSSGERQLIAFARTLYSDPKILILDEATSHI
                     DTETEEIIQHAMEVVKEGRTTFIIAHRLSTIQNADQILVLDQGRIIEHGKHEELVARG
                     GVYAQMHEIQAKV"
     gene            4144..4839
                     /locus_tag="ACHU66_000363"
     CDS             4144..4839
                     /locus_tag="ACHU66_000363"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFR3388678.1"
                     /translation="MKALIFDVDDTLYDQIQPFERALERHIEVAREQIEPLYLSFRRY
                     ADEVFEATAIGKMSLKDSHIYRMKHALADFGYQVSDATALAIQIDYDYFQGQIELSPV
                     FPEIFSWCQAQGIAMGIITNGPYRHQLRKIRTMGLVNWFELEHVLISGQVGITKPNPA
                     IFQLMEERLGMSGEDICYLGDSFENDVVGAKAANWKAIWFNHRKRVEPTAPYQADKVV
                     TDWDELVEVIQSF"
     gene            4993..5562
                     /locus_tag="ACHU66_000364"
     CDS             4993..5562
                     /locus_tag="ACHU66_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR3388679.1"
                     /translation="MELPMTQAFFPIHTLETVSPELRENLATVKKNNGGYIPNLIGLL
                     ANSPTALETYQTVSGINRRSSLNPTEREVVQITAAVANGCGFCVAGHTAISIKQVKMP
                     DVILQALRQGTPIETDAKLDALARFTLAVIHEKGKVEQPLLEEFFQEGYTAENALDVV
                     LGVSLATLCNYANNLINTPINPELQAYAL"
     gene            5961..6662
                     /locus_tag="ACHU66_000365"
     CDS             5961..6662
                     /locus_tag="ACHU66_000365"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR3388680.1"
                     /translation="MGKISKEALKEQIKDGIIISCQALPGEPLYREEGGIMPLLVKAA
                     QEAGAVGIRANSVRDIKEIKEVTTLPIIGIIKRDYPPQEPFITATMREVDELAALDIE
                     VIALDCTKRERYDGLDIVDFINQIKEKYPEQLFMADISTFEEGLTAYEAGIDFIGTTL
                     SGYTSYSRQEEGPDIELVDRLCRAGIDVIAEGKIHYPDQVKIIHDLGVAGIVVGGAIT
                     RPKEIAERFISALHK"
     gene            6690..8198
                     /locus_tag="ACHU66_000366"
     CDS             6690..8198
                     /locus_tag="ACHU66_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFR3388681.1"
                     /translation="MNFKKFSQLGQAFMLPISILPVAGLLLGIGGALSNSNAVAQFPV
                     LDQAWLQAVFTIMSTAGSAVFANLALIFAIGVAVGLADADKGTAGLSAGVAYLVYTAT
                     ISGFLTLFADKGASIDTGVLGALAIGITVATLHNKYRKIELPAFLGFFGGSRFIPIIS
                     SFAAIAIGSIFYIIWPPIQNLLVDFGKAIAEMGSFGTFLYGFGLRLTGAFGLHHTIYP
                     MFWYTSLGGSEVVAGETIQGAQNIFFAQLADANHTGLFTYGTRFFAGRFATMIFGLPA
                     SCLAMYHAIPKENRKKVGSFYSSSALTSLLTGITEPIEFSFLFVAPWLYVVHAFLDGL
                     SFLVADLLQIRIGNSFSGGLIDYLIFGVFQGNDKTNWIYVIPVGLLWAAVYYLVFKFL
                     VTKFHVLVPGMESSDQASHSEKVLSNGSSLRDNAIAIIDALGGADNIENVTACATRLR
                     VSLRNSGLVDKGVIQSIGATAVLDVKGGIQAIFGGKAILYSQEINQILGLEE"
     gene            complement(8302..9114)
                     /locus_tag="ACHU66_000367"
     CDS             complement(8302..9114)
                     /locus_tag="ACHU66_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR3388682.1"
                     /translation="MLQTPKHITAIIESHLDQMTRLEKQIASYFTQLDPSSADLSQEN
                     TIQQLHISPSALTRFAKKCGFSGYREFVFAFQNNQQYLDKHFEKLQRSLTKKVLVDYE
                     EILTATNKLVDEEKLEEIAKLIDSSKRVYFYGIGSSGLVAMEIKSRFMRLGVVCDAIT
                     DENNLIWTTNILDDSCLVIGLSLSGQTEEVMEHLQLAAQKGIPTALLTTKSFPHPTFD
                     QIIPVASVRHLNYGNRISPQIPLLIMLDIIYAYFLAIDKEKKENIFKQTIRE"
     gene            9214..9711
                     /gene="ybeY"
                     /locus_tag="ACHU66_000368"
     CDS             9214..9711
                     /gene="ybeY"
                     /locus_tag="ACHU66_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR3388683.1"
                     /translation="MYIEMIDETGQVSAQMQEQITELLQFAAEKIGKQNKEMAVTFVD
                     NKRVHEVNLEYRGIDRPTDVVSLEYKPESEIVFDEEDLLDNPELAEMMEDFDAYIGEL
                     YISIDKAREQAEDYGHSYEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRE"
     gene            9692..10093
                     /locus_tag="ACHU66_000369"
     CDS             9692..10093
                     /locus_tag="ACHU66_000369"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFR3388684.1"
                     /translation="MDLQENNSKNRWKNRELIASLEFAVTGLLTAFKEERNMKKHLAS
                     AILVVLAGLIFQVSVTEWLFLLLSISLVIAFEIVNSAIENVVDLASDYHFSMLAKNAK
                     DMAAGAVLFVSGFALLTGLIIFVPKIWDFIF"
     gene            10110..11009
                     /gene="era"
                     /locus_tag="ACHU66_000370"
     CDS             10110..11009
                     /gene="era"
                     /locus_tag="ACHU66_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR3388685.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNYVMGQKIAIMSDKAQTTRNKI
                     MGIYTTEEEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEKRGKGD
                     DMIMERLKQAKVPVILVVNKIDKVHPDQLLEQIDDFRQQMDFKEIVPISATQGNNVNR
                     LMEILKENLDEGFQYFPADQITDHPERFLVSEMIREKVLHLTREEIPHSVAVVIESMK
                     RDEFTDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            11697..12524
                     /gene="mutM"
                     /locus_tag="ACHU66_000371"
     CDS             11697..12524
                     /gene="mutM"
                     /locus_tag="ACHU66_000371"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFR3388686.1"
                     /translation="MPELPEVETVRRGLNRLVKGKIISKVEVTYAPMIKTGVDAFCQD
                     LIGQEILDVDRRGKYLLIYLTDHVLISHLRMEGKYNFFPDQVPANKHFHAFFTFSDGS
                     TLVYQDVRKFGTMELLGKADVDAYFISRKIGPEPTEEDFDLEEFAKKLAKSRKPIKSH
                     LLDQSLVAGLGNIYVDEVLFKAKVHPAQTSNQLSAEQVADLRQATIEVLQLGIEKGGS
                     TIRTYKNALGMDGTMQDYLQVYGKTGQACPRCQTEIVKIQLGGRGTHFCPKCQVKHG"
     gene            12517..13119
                     /gene="coaE"
                     /locus_tag="ACHU66_000372"
     CDS             12517..13119
                     /gene="coaE"
                     /locus_tag="ACHU66_000372"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637643.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR3388687.1"
                     /translation="MAKIIGLTGGIASGKSMVTAFLREQGYSVIDADAVVHELQAKGG
                     KLYQVLVKEFGQDILSDDGNLDRAKLGQAVFADSKLRARLSDLQDQIIRQELLDRRDA
                     LKQTEDVIFMDIPLLYEADYSGEVNEVWLVYVDRAQQLERLMKRNGLAVQDAENRMAA
                     QLSLEEKRSQAQVVIDNSGAVEATLVQVARLLEELKDGRR"
     gene            13106..14311
                     /locus_tag="ACHU66_000373"
     CDS             13106..14311
                     /locus_tag="ACHU66_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFR3388688.1"
                     /translation="MDDGSSYWKQNLKVAWLGNFLTGTSFTLVMPFISVFVEELGVGP
                     GQVEYYAGLAVSVNALAAALMAPIWGSLADRYGRKPMMVRAAFAMIFTMGGMAFVPNV
                     FWLLVLRVLNGVFTGYIPNATALIASQVPKDKTGYALGTLSTGAVAGNLIGPTLGGIL
                     AEMFGVHMVFLLVGLLYAIVVLLTVFYIREDFVPIKKGEEMSVKEVFEQVKDRQMLVG
                     LFVTSMIIIAAAQAVVPILTLYVRHLGQTDNLLFVAGFIISLPGMASLVTSGYLGKIG
                     DHIGNHRLLLIALTYSLLINVFCVFAENPFQLGLLRFMYGFGTGALLPSVNSLLTKLT
                     PKEGISRIFSYNQLFNNLGSVVGPMMGSAVAAHMGYDWVFYLSSGLVLFNLIWSLTNF
                     RNYLKVRDV"
     gene            14311..14460
                     /gene="rpmG"
                     /locus_tag="ACHU66_000374"
     CDS             14311..14460
                     /gene="rpmG"
                     /locus_tag="ACHU66_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR3388689.1"
                     /translation="MRVKINLQCSECGSKNYLTSKNAKTHPDKIEVLKYCPKERKVTL
                     HLETK"
     gene            14507..14740
                     /gene="secG"
                     /locus_tag="ACHU66_000375"
     CDS             14507..14740
                     /gene="secG"
                     /locus_tag="ACHU66_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR3388690.1"
                     /translation="MYQALLAILLILSVILIVVIFIQPAKNQSSNVFDSSSGALFERT
                     KARGFEAVMQRITAILVFLWMLDALALVIISSK"
     gene            15171..17540
                     /gene="rnr"
                     /locus_tag="ACHU66_000376"
     CDS             15171..17540
                     /gene="rnr"
                     /locus_tag="ACHU66_000376"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR3388691.1"
                     /translation="MKNDIINYIKEVGPVTMDQLADQFGASSAKSFTDLVKLVSSMEG
                     SRQLVFDNAGRITLPAPKISKNKVTLQGIFRAHKSGFGFVTIDEEEDDLFVSRDDVNF
                     AIEGDRVEIAIKKVADRLKGTAAEAEVIDILEHSLKTAVGLIVFDEDKPEYAGYIKSK
                     NQKIAQKIYIKKSPLVLTGTEILKVDIEAYPNKKRDHFVATIRDVVGHKDDVGIDVLE
                     VLESMDIVSEFPDEVLAEANRVPESPSEQDFAGRLDLRDEIIFTIDGADAKDLDDAVH
                     IKQLKNGNLELGVHIADVSYYVTEGSALDQEAIKRGTSVYVTDRVVPMLPERLSNGIC
                     SLNPNVDRLTQSAIMEIDRKGKVVKHWIGQTVIKTNFRMTYSDVNDMIAGNQEKLDKY
                     KTIVPSVELMVKLHETLETMRYKRGALNFDTAEAKIIVNKDGLPVDIQLRQRGIAERM
                     IESFMLVANECVAEHFAKLDLPFIYRIHEEPKSDKLQKFIDYATSFGLTVYGTASSIS
                     QDALQDLMERVKDEPYADVLNMMLLRSMQQARYSEHNHGHYGLGAEFYTHFTSPIRRY
                     PDLLVHRMVRDYGHNPAEKAEHFEQVIPELAKSSSSLERRAIEAEREVEAMKKAEFMQ
                     EFVGQEFDGVVSSVVKFGLFVELPNTVEGLIHITNLNEYYQFHERTLTLQGEKSGRVF
                     RVGQPIRIKLTRADKMTGEIDFAHVPSELDIVEKALKAKRRTASHSNRDRDDRSGRGR
                     GKHHKQEAAGRDSGRHKSGKDHKSKSGKKDKKKKPFYKEVAKKNKKKRR"
     gene            17543..18010
                     /gene="smpB"
                     /locus_tag="ACHU66_000377"
     CDS             17543..18010
                     /gene="smpB"
                     /locus_tag="ACHU66_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR3388692.1"
                     /translation="MAKGEGNVIAQNKKARHDYTIVDTVEAGMVLTGTEIKSIRAGRI
                     NLKDGFAQIKQGEAWLVNVHIAPYDEGNIWNQEPTRTRKLLLRKKQIESLGNEVKGTG
                     MTLVPLKVYIKDGFAKVLIGLAKGKHDYDKRESIKRREQDRDIKRTMKAINSR"
     gene            complement(18097..19140)
                     /locus_tag="ACHU66_000378"
     CDS             complement(18097..19140)
                     /locus_tag="ACHU66_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HFR3388693.1"
                     /translation="MKTAIYEKSGKMTVAEIEKPSLQAKDDVIIKVVRACVCGSDLWG
                     YGEDIHHDHGDTNSGHEAIGIVEEVGEDITTLVPGDFVIVPFTHGCGHCDACRAGFDG
                     TCDNHPGYTNWGNNYQSQYIRFHYGNWALVKIPGQPSDYSEGMIKSLLTLADVMPTGY
                     HADRVADVKPGDKVVVIGDGAVGQCAVIAAKMRGASQIVMMSRHTDRQQMALESGATA
                     IVAERGEEGIAKVREILGGGADAALECVGTEASIDQALGVLHNGGRIGFVGVPHYNNH
                     ALGSTFAQNIIIGGGSASVTTYDKEILLDAVLKGDINPGRVFTQTYSLDNIDQAYKDM
                     ANRKTIKAMITVD"
     gene            19294..20154
                     /gene="tehB"
                     /locus_tag="ACHU66_000379"
     CDS             19294..20154
                     /gene="tehB"
                     /locus_tag="ACHU66_000379"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFR3388694.1"
                     /translation="MTVLVPYKRMPIWNQDTVPQHFLTKHNTKVGTWAKIKVLRGQLK
                     FEPISDDNEILGEYVYGPTADIPFVEPQAWHRVTLLTEDTEFFLEFFCQPEDYFAKKY
                     DYTRTHSEVLEALKRIEPCKVLDLGCGHGRNSLYLAQQGFEVTAVDKDVPSIRTLLQV
                     KEVEDLDLQAGTYDINSASLEQDYDWIISTVVFMFLERDRVPAIIRNMQEQTRPGGYN
                     LIVAAMDTEDAPCPMNFSFTFGVGELKEYYRDWELVKYNEDFGQLHKRDENGHFLKMR
                     FATMLARKKG"
     gene            20348..22171
                     /locus_tag="ACHU66_000380"
     CDS             20348..22171
                     /locus_tag="ACHU66_000380"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HFR3388695.1"
                     /translation="MKKTFVRIATFLMLVCLFPVQLVQACSGFIIGKGLTTDGSILYG
                     RTEDYPYPPDNGAHNKNYIVVPAAAYAKGDMLVDESFGFTAPHLANEFKYTSTPDAAR
                     GDGSNGNFGAHGFNEKGVSMTATVTAIPNKKVLAVDPLVTAGGLGEAILIDYVLPRVT
                     SAREGIELIAKTIDEKGSAEGNIIVIADKNEVWYMEILSGHQYVAIKFPEDKYAIFAN
                     TYYLGHVDFTDTENVIASAKVEEVAKQAENYMMVDGKFHIAKSYGPENYADGDRSRTY
                     AGIKLLDPASSVTYEDAVYDLLRQPTDPSRRFSLQDTFALQRNRFEHLPEFRPDDEAG
                     KVKQGDNGANDQAADATYKYALGNENVIDAHVYQINSSLPSAFGGTVWLGLAQTRNTP
                     YVPFYGIVTDTYEAFKNRSASYDTNSWYWTVQNIDKMAISHPELFGTSIQEKWIALEK
                     EWIASQAALDAQYAGLSEDAAVALAPTVTEATLARSAEIFAQLKAVEAEMMAKIEAAT
                     TPSSSSTEPSTSTEPSSSGTETSTSTSQSTSDSNTGGATDTSSSSGTVVPSDKKVTPT
                     NKKGKSNLPSTGEQVSILLVALGVAGILTAIFLHRKKSSKE"
     gene            22292..24742
                     /locus_tag="ACHU66_000381"
     CDS             22292..24742
                     /locus_tag="ACHU66_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFR3388696.1"
                     /translation="MKQASYPIQGMTCASCALTVEKAVGKLIGIEEASVNLATEKLSV
                     SYDEKLLGLEDIRQAVEKAGYQLVDNLVTESYDISGMTCASCALTVEKALGKLEGVEE
                     VSVNLATEKATIRYSRHRQNPASLERAVEQAGYQLIRPEKVEGAADKGPSKEEKLWHR
                     FVWSAAFTLPLLYIAMGPMLPWGGLPLPALLHQPLVYAISQVILLIPILYIGRSFFQK
                     GFKTLLQGHPNMDSLIAVGTGAALVQGLLMIAFLLMGKEVAMHGHHPELYFESAAVIL
                     TLITLGKYFEARAKGQTSEAIKKLMNLAPKTAQVLRNGQEIQVPIEEVVVGDQVVVRP
                     GQQIPVDGQVLEGQTRVDESMLTGESLPVKKALGDNVFGGTLNQQGAITMQATKVGRD
                     TTLAQIIRLVEEAQGSKAPIAKLADQVSAIFVPVVMGLALLSGLAWYFLGQESWIFSL
                     SIIIAVLVIACPCALGLATPTAIMVGTGKGAENGLLFKSGQAIETLQGVNTIVFDKTG
                     TITEGKPQVTDVHLLSTKNREQVLQLAASSEQFSEHPLAQALLQAAQTEKIDLLPATD
                     FQALSGRGLSVIIAEQTIYLGNERLMREQGIDVSKGRAVAEAFAQQAKTPVFLASQQE
                     VLAVIAIADKIKETSRQAVQALQTIGLEVVMLTGDNEKTAKAIAKEVGIEQVISQVLP
                     DDKANQVKHLQEQGKTVAMVGDGINDAPALAQAHVGLAIGSGTDIAIESADIVLMHSD
                     ILDVVKAVKLSQATMRTIKQNLFWAFAYNVIGIPVAMGLLHVFGGPLLNPMFAGAAMA
                     LSSVSVVLNALRLKTYKL"
     gene            24821..25306
                     /gene="tpx"
                     /locus_tag="ACHU66_000382"
     CDS             24821..25306
                     /gene="tpx"
                     /locus_tag="ACHU66_000382"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR3388697.1"
                     /translation="MTTFIGKPVTLVGPQLRVGDVAPDFVLMANDLSLKSLKDFGKQT
                     KVISVVPSIDTGICDTQTRRFNQELADRDNTVVITVSADLPFAQKRWCGAAGLEDAIT
                     LSDYYDHAFGKSYGVLMREWNLLARAVFVLNADNKITYVEYLDNVNEHPDYDAALEAV
                     K"
     gene            25401..25736
                     /locus_tag="ACHU66_000383"
     CDS             25401..25736
                     /locus_tag="ACHU66_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HFR3388698.1"
                     /translation="MEKTQIIQDVLALIQDLNRAFQADKVGTADEQRFQHNLTETTRI
                     LSEAESVTNTELIAIEKFYRSTSFLVGLGDLRLSETSRQAWRAFDRYYYETIREELKL
                     YGGSAIAQV"
     gene            25825..26526
                     /locus_tag="ACHU66_000384"
     CDS             25825..26526
                     /locus_tag="ACHU66_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3388699.1"
                     /translation="MSYIQVKNSSKYYQMGDATIVANDGVSFEIEQGELVIILGSSGA
                     GKSTLLNILGGMDSNDEGEVWIDGVDIAKLSSHELTNYRRDDVGFVFQFYNLVANLTA
                     KENVELAAEIVKDALDAEEVLEQVGLGHRINNFPAQLSGGEQQRVAIARAVAKKPKIL
                     LCDEPTGALDYQTGKQVLQILQDMSRTQGTTVIIVTHNSSLAPIADRVIRMRDARVHS
                     VELHAEPQDIASLEY"
     gene            26538..29903
                     /locus_tag="ACHU66_000385"
     CDS             26538..29903
                     /locus_tag="ACHU66_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFR3388700.1"
                     /translation="MKKKIYWKDMRQSLLSSKGRFLSIFSLMMLGALALTGLKVTAPN
                     MEKTAQAYIASHRTMDLAVISGLGLSQADLDELETIEGATLEAGYFKDVVTNEGQTAI
                     RLFSAPQTLSTYKLVEGEMPSQKGQIALSASFKNRYKLDDTVQVTESDKDGKILTQTS
                     FTLVGFVASAEIWDNETMGMAASGDGQLGGYGLVSQDTFKSEVYTIARIRYDDLVDLP
                     YYSQAYQDRLDQHQEDVEKLLANNDEQRLAAIQSDGQTDISKGEEEIAQAQSQLEQAE
                     SELETGEEQLATGRREFARGRAKVVQTEAELRAAQAQLLKNKFELEESKKELDGRKAQ
                     LEQTASFLDGYQMELETSAQQLEEAKQELDSQNAQLSQTASQISTGRASWFQAQQELD
                     LEIANHLQEGQTLSDYPDLLARQEGLDVEKSRLDQMESAHEQANQAYLQGYDYYQTKQ
                     NEYNSNLAQYQTWNQEYQAGLARYQAGLSQYEQGIAAYNKGVEDYEWGLSQLESSNQL
                     LRQEEFRLEEADKELSQAQSQFSEKKATADQEISQAQTEIAQAKSDLSKLEKAPYQVY
                     TRSSLPGGDGYTTYSNATRSIAAVGNVFPVVLYLVAALVTFTTMARFVDEERTQSGLF
                     KALGYTNRQIMAKFILYGLTAGLVGTIVGIIAGNLLLSPLISNIITQTTVIGPAKLHF
                     YPLWTGLALLLSLASSVLPAYLVARRELTEKPAQLLLPKPPVTGSSIWLEKWPAIWSR
                     LSFTHKVTARNIFRYKLRMLMTIFGVAGTVALLFGGLGIRSSISGVVQRQFGELIHYD
                     MLVVENSRATEEELDKLTHFLQSDQVRQSLPVAFEQLNQTVEEDGQRKNLSISLYISD
                     RRDFGNQVSLESSTGQPIKLSDRGIVLTEKLAQIYGVSVGDKLSLTLEDKEVSVRVEA
                     VADMYAGHFIYMTDSYYEQVTGKQKTANAYLVQLKDSQLGHIQTLASQLLAMPAVRSL
                     VQNTSLIDMLTTIAGSLQTIMTILVILSILLGLVILYNLTIINMSERIRELSTIKVLG
                     FHNKEVTMYIYRETIVLSLIGMLVGLVGGIYLHKLLLAMIGSDSIRFNPSVGLEVYLI
                     PILAISGILAVLGWYVNHHLRKVDMLEALKSVD"
     gene            complement(30368..31532)
                     /locus_tag="ACHU66_000386"
                     /pseudo
     CDS             complement(30368..31532)
                     /locus_tag="ACHU66_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            31825..33132
                     /locus_tag="ACHU66_000387"
     CDS             31825..33132
                     /locus_tag="ACHU66_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388701.1"
                     /translation="MKKISDLGLTGRKLVGEGLILVFIGIGFLIAGWQFPGLILRFVH
                     AGLFFLALYELSMTFFRKKKSSESVIALVGKAVLFGILASLDLAVQIPLYFAAIFVGI
                     YQLFTALINFITFYLYRKDGVQPRIRYLIDGIWLSLLGSASLFVSGTQLVVQTIVIGG
                     YLILYGLTNLRDGFLFEEAIEQQSLKRHVRLTLPLFLAALVPRMTLQKVNDYLADNEG
                     QTAQSIYNRHKEIAEFPALEVFVHVGEEGFGAVGHVDLSYKGQVYGFGSYDVLSERLG
                     GAIGDGVLFRAERQAYIDFCNQEGMTMLGYQLALSSEQEKAVETRLAEIEGLLLPWQP
                     SAEKVSRRSDGQPIEMYAYRMKEAIGAVLFKFKKSKFKTYFVLSTNCVLLADSVIGQA
                     GTDVLGLRGFIAPGTYQSYLDQEYEKPHSLVVAKNIYYRKEKS"
     gene            33208..35844
                     /gene="polA"
                     /locus_tag="ACHU66_000388"
     CDS             33208..35844
                     /gene="polA"
                     /locus_tag="ACHU66_000388"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR3388702.1"
                     /translation="MKKNKLLLIDGSSVAFRAFFALYNQIDRFKSPTGLHTNAIYGFN
                     LMLDHMMKRIEPTHILVAFDAGKTTFRTEMYADYKAGRAKTPDEFREQFPFIRQMLDA
                     MGVKHYELAQYEADDIIGTLDKMAERTEIPFDVTIVSGDKDLIQLTDENTVVEISKKG
                     VAEFEEFTPAYLMEKMGITPTQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLTEFGSL
                     DGIYENIDSMKASKMKENLIADKEKAFLSRTLATIDTQAPIEIGLDDIVYQGPKVDEL
                     GQFYDDMGFKQLRAQLGTTSSQEEAVLDFQIVTEISPAMLKQDQFFYFEILGENYHRE
                     DLVGLAWGDKEKIYVGGPELLDSPVLRDFLENQTIKTYDFKRGKVLLDRKEITLPPAT
                     FDSRLAKYLLSTVEDNSLTTIANLYGQISLVPDEAVYGKGAKLDLPEREIFFPHLARK
                     VQVLIETEEPMLTKLEENQQLDLLFDMELPLANVLAKMEIAGIKVEAETLKAMQSENE
                     VLIDQLTKEIYELAGQEFNINSPKQLGTILFEEMGLPLEYTKKTKTGYSTAVDVLERL
                     APIAPVVSKILEYRQITKLQSTYVVGLQDAILEDGKIHTRYVQDLTQTGRLSSTDPNL
                     QNIPVRLEQGRLIRKAFVPSLENSVLLASDYSQIELRVLAHISQDKHLIEAFQQGADI
                     HTSTAMRVFGIEKAEDVTPNDRRNAKAVNFGVVYGISDFGLSNNLGITRKEAKAYIDT
                     YFERFPGIKNYMETIVREARDKGYVETIYKRRRELPDINSRNFNVRNFAERTAINSPI
                     QGSAADILKVAMINLDKALTEAGLATRMLLQVHDEIVLEVPVAELETVKAMVKETMES
                     AISLSVPLIADENEGSTWYEAK"
     gene            35831..36613
                     /locus_tag="ACHU66_000389"
     CDS             35831..36613
                     /locus_tag="ACHU66_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR3388703.1"
                     /translation="MKQSKKGKSMKKLILCIGLCSLLLAGCGKASQKTEKTPASSGEE
                     FSTTLPILQEKQDTTNEQFNVLANAEVVAVNSTPPADETQKGHKIYAANNGVAEKDEN
                     GNIKEYFRYYNVPSTWTINAENTKDETVAAVYDVKEGGSNYMVQLYNINAFNQSPLED
                     GRNMTPEELEARMVETNHSFNEKTVVTINGQEWRVGRQLLTDQKLARLTFYRMESTAA
                     YDDSVVVGSIYYSLDPGTDKDRTNLKKVIGQVKDVVYQIAKK"
     gene            36733..37170
                     /locus_tag="ACHU66_000390"
     CDS             36733..37170
                     /locus_tag="ACHU66_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR3388704.1"
                     /translation="MSYSFQNPSQDIIFDYLKNAKTIAVVGLSSREETAAYRVSKLMQ
                     EAGYKIIPVNPKAAGGTILGELVYSSLAEIDQPIDIVDVFRRSEFLPEVAQEFIQSNA
                     KIFWAQLGLESQEAEKLLRQAGRNDIVMNKCIKIEYLEMKEQY"
BASE COUNT        10566 a         7287 c         8909 g        10595 t
ORIGIN      
        1 gtagagcgca tccatggtaa ggatgaggtc gccggttcaa tcccggcaac tagcataaaa
       61 tactatcgaa aggccttgtt tgacaaggtt tttcttattt ttgtcctaaa ttcgccctta
      121 ttgtcaaaaa atcccttgac atgaatgttt attatggtag aatagggctt agaaatatga
      181 aaggagtaaa acaaatgaac aagcaacttg ttattcagca agctattttt tatcatacac
      241 tcctttctaa gcaggaaaac aaagctcatt gatgaacttt tgtcgttttt cgtgggctat
      301 attttgaaac attgagacaa tcaagcactg tgggtatggt gtgataaaaa ttgtcgacta
      361 tgttcaagag ctggtctatt tttgtggact tacgcaagcc taggtggagt gagatgcttc
      421 cctaggcttt tttgtgcgct tagaaaggta atactcttcg aaaatcaaaa ttatccgttg
      481 tcaacttgcc ttgatgaact ccagttctat cttcgacatc gttttctagg ctacttttga
      541 ttttcattga gtataacgtc tatcaattgt tgcttattga ataaagcggc aaatgatagg
      601 ttatagattt gtaaaggaga tatatgtttc gattaatttt tgactatatc aagcgtcata
      661 agtggctcta tctattggtt gctgtgacct tgattattta tgacgccaca cttttgctac
      721 caacgcaaat cattcagcgg atggtagata tactaaccaa gaatgaatta acacaggcca
      781 tattggttca ggagatgacc ttattgttat tggtaacagt actgaactat gcaacagcct
      841 tcatttggca tctgaaatta tttcaggctt ctgtcaattt caagtttgat atgcagcaac
      901 gtgcctttaa aaaattagtg accatgcgga ctccatttta tgaaaaattc cgctcaggag
      961 atgtcatgac tcgtttttca acggatgtgg atggcctgat ggagatggtg ggttacggtc
     1021 ttatgattgt cgtctatgca ggcggtatgt tggcttttat cattcccacg atgttcttta
     1081 ttgattggaa aatttcgcta gtagctttac taccgatgct gttcatgaca ctatgtatat
     1141 tttttatcgg gagaaaacaa gataaggcga ttgatgccaa tagagaagct gtagcccagc
     1201 tcaataatga agttttggag gtcatcgaag gcattcgagt gactcgggct tatagtaaaa
     1261 aagcaactca aaaggctcaa tttcaagctc gtaccaagca attggctcaa ggtggggatc
     1321 gcatcacttc cttgcaatca ttgtataatc cattagccac agtctgtttg ggattgtcta
     1381 ctattttcgt gcttctcatg ggggcacaag ccgtgaaagc agggcaactg accttgggac
     1441 aagtaattgc ccttcaattg tatgtcggtt cgcttttgga gccgttttgg acactagcag
     1501 atttcatcct tgtttatcag actggtaaga catctttcga aaaactgcaa gagctgattg
     1561 agacgggtga tgatttggag gctgatggtt ctaaggaaat tgcagagctg tctagtattt
     1621 ccttcaaaaa ttatagcttt agttatccac aggcggaaag agccagccta caggacatca
     1681 attggacact gaaagcaggg caaactgtag gtatcgttgg gaaaactggt tcgggaaaaa
     1741 cgaccttggt tcgccaattc ttacgccagt atccgattgg tcagggaaat tttttcatca
     1801 atcatcaatc tgttttagac tttaaacgtt ctagtataga ggaaaaaatt ggctatgttc
     1861 cccaagaaca cattttgttt tcaaggtctg ttggagaaaa tattgccctt gggaaagtag
     1921 ctagtagctc tgaggagatt gaacaagcca ttgccacagc agcttttagt caagatttaa
     1981 aacggatgac tgatggtctt gataccatga ttggtgagcg tggtgtgtct atttctggcg
     2041 gtcagaaaca acggatttcc attgcccgtg ctttcctgcg agagccagat ttgctgattc
     2101 tagatgattc tttgtcggca gtggatgcta ggacagaacg ccagattatc caaaatattc
     2161 aaaaagaacg tgcaggcaaa accaatgtca tcgtgaccca tcgtctatct gctgtcaacc
     2221 atgcagactg ggtactagtc ttggatgagg ggcgtatcgt tgaggaaggg cgtccagctg
     2281 atttgcttgc ccaaagaggt tggtactatg aacaatacca acgacaacaa agtcaggaag
     2341 gaggagagta atgactattt taggtttttt attaaaacaa atccgtcgtg ttaagtggct
     2401 atttatagta gctgtcggct tctatctatt ggcttcgacc atggttcgat tggctcctct
     2461 actgattcag caggcgattg atgggccgat tacggatttg agcaagggtt tgccttttga
     2521 tgaagctgtt ttcttaaacc agtcagctca atacatggga atgattattc tgggagctat
     2581 tggcttctac ttatctatgc gtctactcat gcattgcgcc aatcgtattg ctgaaaatct
     2641 acgtaatcaa gcttatgatg ttatgcaacg gttgcctatt tcttattttg atgataagcc
     2701 cgctggaaaa attgcaacta gaattgtcaa tgatacagaa actctgcgga cacaatttta
     2761 tgggacctta gttaatgcct ttaacaatat tgttcgtttg ctctttacat acggtgtcct
     2821 attttacatg aataggagtc ttggttggct gatgttactc ttgataccac tctatatcgg
     2881 tattcaattt gcttacaaga aaatgacaga caagccgatg aaggattttt atgatgcaag
     2941 gagtgacgtc aatacgcagg tgaatgagac catgaatggt gcaagtttga ttcaactttt
     3001 tggacaagaa gagcgcatca tgaaagaatt tgaagtgact gctgacaaga tgcgtcgggc
     3061 agataataag attatctggg cccagtctct tgccacgtgg aatctgagtg gttttcttca
     3121 aaatttggtc attgctgcga ttttaacggt agtcggctat cagtttcttg cgggacagta
     3181 tggagttaca gctgggaaac tctttgttta tgtcaactat atcgaaggag tcttcattgc
     3241 tttgggagcg ctggttcaac agttcccaaa tatgcttcgc tcgtttgaaa caggtaagcg
     3301 tctaatggcc cttctagaag aagaggtgga ggacgactgt gatcgtattt tggaagttga
     3361 acaagggcaa gtcgtgtttg aacacgtgaa ctttagctat gaagaaaaca gaccagtttt
     3421 gaaagatatt accattcggg cagaaaaagg agaaacagtt gctctggttg ggcatactgg
     3481 gtctggtaag tcttctatta tgaacctcct ctatcgtttc tatgatccac aggagggacg
     3541 tgtactgatt gatgggaaaa acatccgtga ttattctcgt gagagcctgc gcagtcacat
     3601 ggggattgtt ttgcaagatc cctatctctt tacagggacg attgctagca atgtttccat
     3661 gaatgaagag gaagcagata gaacaagaat catgcaggcg ttagaaaaag ttggtgcagg
     3721 gcctatgcta tctcgcttag agaaagggat tgatgaaccg gttgttgaga agggggcggc
     3781 cttttcaagt ggggaacgtc agttgattgc ctttgcgcgg acactctact ctgatcctaa
     3841 aattctgata ttggatgaag caacttctca tattgatacg gagacggaag aaattatcca
     3901 acatgctatg gaagtggtga aagaaggtcg gacaaccttt atcattgccc accgactttc
     3961 taccattcaa aatgcagacc agattcttgt cttagatcaa ggccgaatta ttgaacatgg
     4021 gaagcatgaa gaattggttg cacgtggtgg agtatatgct caaatgcatg agattcaagc
     4081 aaaggtgtaa taagcagtta aaaattccaa gctttggctg gaattttttg ctataattga
     4141 ctcatgaaag ccttaatttt tgatgtagat gatacgcttt atgatcagat tcaaccgttt
     4201 gaacgtgctt tggaaagaca cattgaagta gcaagggagc aaattgaacc actctacctt
     4261 tcctttcgca gatatgcgga tgaagtattt gaagcgacag caattggaaa gatgagtctg
     4321 aaagacagtc atatttatcg gatgaaacat gctttggcag attttgggta tcaggtgtct
     4381 gatgcaacag ccttagctat tcaaatagat tatgattatt ttcaagggca gatagagctc
     4441 agtccagtgt ttccagaaat attttcatgg tgtcaggcgc aaggaattgc aatggggatt
     4501 attacgaatg gaccttatag acatcagttg agaaagatac gcacgatggg gttagttaat
     4561 tggtttgagc tagaacatgt cctaatttct ggacaagttg gtatcacaaa gccaaatcct
     4621 gctatttttc aactaatgga agaacgtttg gggatgtcag gcgaagatat ttgttatcta
     4681 ggggattcgt ttgaaaatga tgtggtagga gcgaaagctg cgaattggaa ggcaatttgg
     4741 tttaatcata gaaagagagt agagccaaca gctccgtatc aggcagataa agtggtgaca
     4801 gattgggatg aactggtcga agttatccag tctttctagt ggtttctaca agggattatc
     4861 aattttttat aggtgtgata ggtaaaatat attagcagga tgcctgtctt tatgatagta
     4921 taaaatgcaa gcatagaagt ataggtgtga atccaagcag atttttattc ctactctatg
     4981 aaattttttg aaatggagtt acctatgaca caagctttct ttcctatcca tactctagag
     5041 actgttagtc cagaattacg agaaaacctt gcgactgtca agaaaaataa tggcggttat
     5101 attcctaatc tcatcggtct tttagccaat tctcctacag ccctagaaac ctaccaaact
     5161 gttagtggta tcaatcgccg tagtagtctg aatccgacgg agcgtgaagt ggtacaaatt
     5221 acagctgccg ttgccaatgg ctgtggattt tgcgtggcag gtcatacggc tatttctatc
     5281 aagcaggtta agatgcctga tgttattcta caggctctac gccagggaac tcccatcgaa
     5341 acggatgcaa aattggatgc tctcgctcga tttactctgg ctgttatcca tgaaaaaggc
     5401 aaggttgaac agcccttgct agaggaattt ttccaagagg gctacacggc tgaaaatgcc
     5461 ttggatgtgg tgttaggagt cagtcttgca actctttgta attatgccaa caatctcatc
     5521 aacactccta tcaatccaga attacaggcc tatgctctgt aggagttcca gcagaatatg
     5581 tatcgcaaac tagttaggac gtttacatta tcctaactag tttttttgaa ttaaccaagt
     5641 gtgattttcc taatatacat ttgatactct tcgaaaatca aaattatccg ttgtcaactt
     5701 gccttgatga actccagttc tatcttcggc ttcgtttcct aggctacttt tgattttcat
     5761 tgagtatgag atggcctttg ttttttaaaa ctagttttag aagaggtata aaatgttgaa
     5821 actagtttca aaagaagact attttctttc aaaatagaaa ttagatttta aatgaggaac
     5881 tgggattgaa aaaaagttaa tgtgctatat aataatattg taagcgctat ccagaatccg
     5941 ggaatagaaa ggaaaaatta atgggcaaga tcagtaagga agctctaaaa gaacagataa
     6001 aagatggaat tatcatttcc tgtcaggctt tgccaggcga acctctgtat agggaagaag
     6061 gcggtattat gcctttacta gtcaaggctg cccaagaagc aggggctgtc ggtattcggg
     6121 ccaatagtgt acgagatatt aaggagataa aagaagtaac tactttacca attatcggaa
     6181 ttatcaagcg agactatcct ccacaagagc cctttattac ggctacgatg cgagaagtag
     6241 acgagttagc agctctagat attgaagtca ttgctcttga ttgtaccaag cgtgagcgtt
     6301 atgatggact cgatattgtt gatttcatta atcaaatcaa ggaaaaatac cccgaacaac
     6361 tttttatggc agatatttca acctttgagg aaggattaac tgcttatgaa gcaggcatcg
     6421 atttcattgg gacaacactt tcggggtata catcctatag taggcaggag gagggaccag
     6481 atattgaatt ggttgaccgc ttgtgccgag caggtatcga tgtgatagcg gaaggaaaaa
     6541 tacactatcc tgaccaagta aaaattattc atgatttagg tgtggcagga attgttgtag
     6601 gcggtgcaat cacacgtcca aaggaaatcg cagagcggtt tattagtgct ttacataaat
     6661 aataaatagg ttatagaaag gaatggagta tgaattttaa gaagttttct cagctagggc
     6721 aggcctttat gctgccaatc tcaattttgc ctgttgctgg tctcttactg ggaattggag
     6781 gcgccttatc aaatagtaat gctgtagcac aattccctgt attggatcaa gcgtggttgc
     6841 aagctgtatt cactatcatg agtacggcag gtagtgcggt atttgcgaat ttagcattga
     6901 tttttgcaat tggtgttgct gtaggtttgg cagatgctga taaggggact gctggcctat
     6961 ctgctggggt tgcatacttg gtttatacgg ctactattag tggattttta actctttttg
     7021 cagataaggg agcaagcatt gatactggag ttttgggcgc tttagcgatt ggtatcactg
     7081 ttgcaacttt acacaataaa tatcgaaaaa ttgaattacc agcctttctt ggtttcttcg
     7141 ggggctctcg ctttattcca attatttcat catttgcagc aattgcgatt ggctccattt
     7201 tttatataat ttggccaccg attcagaatt tgttagttga ttttggtaag gcaatagcag
     7261 agatgggaag ttttggaact ttcttatatg gttttggatt acgtttgaca ggtgcatttg
     7321 gcttgcacca taccatctat ccaatgtttt ggtatacttc attaggcgga agtgaagtgg
     7381 tagcaggtga gacaatccaa ggtgcgcaaa atattttctt tgctcaatta gcagatgcga
     7441 atcacacagg cttatttact tatggaactc gattttttgc tggtcgattt gcaacgatga
     7501 tttttggtct accagcatct tgtttggcta tgtatcacgc aattcctaag gaaaatcgta
     7561 agaaggttgg cagtttttat tcaagtagtg cattaacttc cctattaaca ggaattaccg
     7621 agccaatcga gttttctttc ttgtttgttg ctccatggct atatgttgtt cacgcatttt
     7681 tagatggact atcattctta gtcgcagatc tcctgcaaat tcgtattggg aactcatttt
     7741 ctggtgggct tattgattac ttgatttttg gtgttttcca aggaaatgac aaaaccaatt
     7801 ggatttacgt gattcctgtt ggcttgcttt gggcagcagt ttattatcta gttttcaaat
     7861 ttttagtaac caaatttcac gtgctagttc ctggtatgga aagtagtgac caagcatcac
     7921 attctgaaaa agttttatca aacggttcta gtcttcgtga taatgcaatt gctatcatcg
     7981 atgctcttgg tggtgcagat aatattgaga atgtaactgc ctgtgcaact cgattaagag
     8041 tatcgctcag aaatagtggt cttgtggata agggagttat tcagtctata ggtgcaacgg
     8101 ctgttttgga tgtaaaagga ggaatacagg ctattttcgg aggtaaggct attctgtata
     8161 gccaagaaat caatcaaatt cttgggcttg aagaatagag ttatatttaa gcgaattaaa
     8221 agcattcaaa ctgtacctat cgggtatttt tgaatgcttt ttgttttatt aagattgttg
     8281 tgaaatagaa agacggatga attattctct aatggtttgc ttgaaaatat tctctttttt
     8341 ctccttgtca atagctaaaa aataggcgta gataatatct agcatgatga gcagggggat
     8401 ttggggtgag atacgattgc cgtaattaag gtggcggaca gaggcgactg gaatgatttg
     8461 gtcaaaagtc ggatggggaa aactcttagt tgttaacagg gcggtaggaa tccccttttg
     8521 agcagccagc tggagatgtt ccatgacctc ttctgtctga ccagacagag aaagaccgat
     8581 aaccaggcaa ctatcatcca gaatattggt agtccagatg agattgtttt catcagtaat
     8641 tgcatcacag actacaccca gtcgcataaa gcgggatttg atttccattg caaccaaacc
     8701 agaactgccg attccgtaaa aatagacacg cttgctactg tcaatcagtt tggcaatttc
     8761 ttccaatttt tcctcatcta ccagtttatt ggtagcagtt aggatttcct catagtctac
     8821 caagactttt tttgttaggc tccgctgtaa tttttcaaaa tgtttatcca agtattgttg
     8881 gttattctga aaggcaaaga caaattctcg ataaccggaa aatccacatt ttttggcaaa
     8941 acgggtaaga gcagatggag aaatgtggag ttgctgtata gtgttttctt ggctaagatc
     9001 ggcgctagat gggtctaact gagtaaaata actggcaatc tgcttttcaa gtctagtcat
     9061 ctgatccaga tgagactcaa tgatggcagt gatgtgtttc ggtgtttgta acattttcca
     9121 ttcctctgat agcttctgag gacattatac cactaaaacc ccctcattga taggttttat
     9181 gatataatgg aaacgtaaaa ggaatgtaag attatgtata ttgaaatgat tgatgaaaca
     9241 gggcaggttt ctgctcaaat gcaggagcag attacggaat tgctccagtt tgctgccgaa
     9301 aaaattggca agcagaataa ggaaatggct gtgacctttg tggacaataa gcgtgtccat
     9361 gaggttaact tggagtatcg aggaattgac cgtccgacag atgttgtcag cctagaatat
     9421 aagccagagt cggagattgt ttttgatgag gaagacttac tggacaatcc tgaattggct
     9481 gaaatgatgg aagatttcga tgcctatatt ggtgaattgt atatttctat cgataaggcg
     9541 cgtgagcaag ctgaagatta tggtcatagc tacgagcgcg aaatgggctt tttagctgta
     9601 cacggttttc tgcatatcaa tggctatgat cattatacgc cagaagaaga agcagaaatg
     9661 tttggtttac aagaagaaat tttgacggcc tatggactta caagagaata attcaaaaaa
     9721 tcgctggaaa aaccgagagc tgattgccag tctagaattt gcagtaacgg gtttgttgac
     9781 ggctttcaag gaagaacgca atatgaagaa gcaccttgct tctgctattc ttgtggtgct
     9841 tgcgggactt attttccaag tatctgtgac agagtggctc ttcctcttgc tcagtattag
     9901 tctggttatc gcttttgaaa ttgtgaattc ggctattgaa aatgtggtgg atttggcttc
     9961 agactatcat ttttctatgt tggcgaaaaa tgccaaggac atggcagcag gggcagtctt
    10021 gtttgtgtca ggatttgcct tgctaacagg attgattatc tttgtgccga aaatttggga
    10081 ctttattttt tagaatataa ggagaataca tgacatttaa gtcaggtttt gtggcgattt
    10141 taggtcgtcc aaacgttggg aaatcaacct ttctcaacta tgtaatgggg caaaaaattg
    10201 ccatcatgag cgacaaggct caaacaaccc gtaacaagat tatgggcatt tatacgacag
    10261 aagaagaaca gattgtcttc attgacacac cgggaattca caagcctaag acagctttgg
    10321 gcgattttat ggtggagtct gcttatagca cccttcgtga agtggacacg gtcctcttta
    10381 tggtgccagc cgatgaaaaa cgaggcaagg gtgatgacat gattatggaa cggctcaagc
    10441 aggccaaggt tccagttatt ctcgttgtta acaagataga caaggttcat ccagaccagc
    10501 ttttggaaca gattgatgat ttccgtcaac agatggactt caaggaaatt gtgccaattt
    10561 ctgctaccca agggaacaat gtcaaccgac ttatggaaat tctcaaggaa aacctggacg
    10621 aaggcttcca gtatttccca gcggaccaaa tcactgacca cccagaacgt ttcttggtat
    10681 ctgagatgat tcgggagaag gttctacacc tgactcgtga ggaaattccg cactctgtgg
    10741 ccgttgtcat cgagtccatg aaacgtgatg aatttaccga caaggtccac attcgtgcta
    10801 cgattatggt agagcgtgat agccagaaag gcatcatcat cggtaagcaa ggagccatgc
    10861 tcaagaaaat cggctccatg gctcgtcgtg atattgaact catgctaggt gacaaggtct
    10921 tcctcgaaac ctgggtcaag gtcaagaaaa actggcgaga caaaaaactc gacctcgccg
    10981 actttggtta taatgagaaa gagtattaaa aacgtccggg ggagtgaaac agtctaggaa
    11041 tagactgttt cagctggtca cctagaaatg tgaaagtgac catttgaaaa cccgagcata
    11101 gaaattggaa ggcgagatag cctggggata ggctgtctaa gtcaatcact tagaaatagt
    11161 tagcagaaaa gccgaaaggc ttttactgcg ttatggagaa atctttttag taaacaagaa
    11221 aggaaagtcg attaggtgtt gtcttgaaca cgcctacgac tctttgaaaa aagataaccg
    11281 tgacctaggt gccgaagcac catcggtcag gtttctattt gctttgtaat tttgttagaa
    11341 aggacgagtg tttgcgtact tgaacccgag ctaaaagctc ggaaaataga tagagctcct
    11401 tatgtgcaag tcacatagcg tcgctctcct aattttacta tatttagaaa ggaaagccga
    11461 ttaggtgttg taattgaaca cgcctaggcc tccttgaaaa tcacaaccgt gacctaggtg
    11521 ccgaagcacc gtcggtcagg tttctatttg ctttgtaatt ttgttagaaa ggacgagtgt
    11581 ttgcgtactt gaacccgagc taaaagctcg gaaaatagat agagctcctt gtgtgcaagt
    11641 cacacagcgt cgctctccta attttcagtc gctttttacg ctcttggtat cgtacattgc
    11701 ccgaattgcc tgaggttgag acggttcgtc gtggtttgaa tcgtttggtc aagggaaaaa
    11761 ttatttccaa ggtggaggtg acctatgctc ccatgattaa gacaggtgtg gatgccttct
    11821 gtcaggactt gattggtcag gagattttag atgtggaccg tcgtggcaag tatctcttga
    11881 tttacctgac agaccatgtc ctcatttctc atttgcggat ggaaggtaag tacaatttct
    11941 ttcctgacca ggtgcctgcc aacaaacatt tccatgcctt ttttactttt tcagatggct
    12001 cgaccttggt ctatcaagat gtccgcaagt ttggcacaat ggaactgctg ggcaaggcag
    12061 atgtggatgc ctattttatc agtcgaaaaa ttggtcctga gccgacggag gaagattttg
    12121 acttagaaga atttgccaaa aagttagcta agtcgagaaa acccattaag tcgcatcttt
    12181 tggaccagtc tttggtggct ggtttgggca atatttatgt cgatgaggtc ttgttcaagg
    12241 ccaaagttca tcctgcacaa acgagtaatc agctatcagc agagcaggtg gcagatctcc
    12301 gccaagctac catagaagtt ctacaactgg gaattgaaaa aggtggttca accattcgga
    12361 cttacaaaaa tgccttgggc atggatggga ctatgcagga ttatttacaa gtttacggaa
    12421 agacagggca ggcctgtccc cgttgccaga cagagattgt caaaatccag ctgggcggac
    12481 gggggacaca tttctgtccc aagtgtcagg tgaaacatgg ctaaaattat cggtttaaca
    12541 ggcggaatcg cctctggaaa atcaatggtc acagcttttt tgcgagaaca gggctattca
    12601 gtcatcgatg cggatgcggt tgttcacgaa ttacaggcca aaggtggcaa gctctatcag
    12661 gttttggtga aagagtttgg tcaagacatc ttatcagatg atggaaattt ggatagggct
    12721 aagctgggcc aggctgtttt tgcggatagc aagttgcggg ctcgcctgtc tgacctgcag
    12781 gaccaaatta tcaggcagga gctgttagac agaagggatg ccctcaagca gacggaggat
    12841 gtgattttta tggatattcc tttgctctac gaggcggatt atagcgggga agtaaatgaa
    12901 gtctggctag tttatgtcga tagagcgcag cagttggaac ggcttatgaa acgcaatggc
    12961 ttagctgttc aagatgcaga aaatcgtatg gctgctcagc tttctttaga ggaaaaacgg
    13021 tcgcaagccc aggtggtgat tgacaatagc ggagcggttg aggcgacctt agtgcaggtt
    13081 gctcggcttt tggaggaatt aaaggatgga cgacggtagt agttattgga agcagaattt
    13141 gaaggtggct tggttgggaa atttcctaac agggacgagt tttaccttgg ttatgccttt
    13201 tatctcggtt tttgtggaag agttgggagt gggccctgga caggtagaat attatgcggg
    13261 gcttgcggta tctgttaatg cccttgcagc agccttgatg gcgcctattt gggggagttt
    13321 ggctgaccgc tatgggcgca agcctatgat ggttcgggcg gcttttgcta tgatctttac
    13381 catgggtgga atggcttttg ttcccaatgt tttctggctt cttgttcttc gtgtacttaa
    13441 cggtgtcttt acgggttata ttccaaatgc gacagctctg attgccagtc aggttccaaa
    13501 ggataagacg gggtatgctc tgggtacctt atcaacaggt gcggtagcgg gtaatttgat
    13561 tggtccgacc ctaggtggta tcttagcaga gatgtttggc gtacatatgg tatttttgct
    13621 ggtgggactt ctttatgcta tcgttgtgtt actgaccgtt ttctatattc gagaggattt
    13681 tgttccaatc aaaaaaggcg aagaaatgtc ggttaaagag gtatttgagc aggtgaaaga
    13741 tcggcagatg ctggttgggc tttttgtgac atctatgatt attattgcag ctgctcaggc
    13801 cgtcgttcct atattgaccc tctatgtgcg gcatttggga cagacagata atctgttatt
    13861 tgttgctggt tttatcattt ccttgcctgg tatggcatcg ctagtgacat caggttattt
    13921 agggaaaatt ggtgatcaca ttggtaatca tcgacttcta ttaattgctt tgacttacag
    13981 tctgttgatt aatgttttct gtgtttttgc agaaaatcct ttccagcttg gtttgttgcg
    14041 attcatgtat ggttttggga caggagcatt gttgccttcg gtcaattctc ttttgaccaa
    14101 gttgaccccc aaagaaggga tttcaaggat attttcctac aaccaactct ttaataatct
    14161 aggttctgtt gttggtccca tgatggggtc tgcggtagct gctcacatgg gttatgactg
    14221 ggttttctat ctgtcgagcg gacttgtcct ctttaacctt atctggtctt tgaccaattt
    14281 tagaaactat ttgaaagtaa gggatgttta gtgagagtta aaatcaattt acaatgttca
    14341 gaatgtggta gtaagaacta cttgactagc aaaaacgcca agactcatcc agataagatt
    14401 gaagtcctta aatactgccc aaaagagaga aaagtaaccc tccaccttga aactaagtag
    14461 gttttatggt ataatgtcat aagttattgt agaaagtgaa aaaaatatgt atcaagcatt
    14521 attagcaatt ctgttgattt tatctgttat tttgattgta gtgattttta ttcaaccggc
    14581 taaaaaccag tctagcaacg tctttgactc ttctagtgga gccttatttg aacgtacaaa
    14641 ggcccgtggt tttgaagcgg tgatgcaacg cattacagct attcttgttt ttctttggat
    14701 gctggatgcg ctggcgcttg ttatcatttc aagtaagtag ataaatcaga ctgggacttg
    14761 ttcttggtct atttttttgg gagtgggcca gaactcgaca agtataaaga gttcgtctgc
    14821 ctaccccgca caagttgaat aggtatggtt cggagcgtta ttcgcgaact attgagattt
    14881 gctttgcaaa tcctatctcc cacctccaac aatcatttga ttgttggagc aatcaaccac
    14941 tgcgtctgga tataacgcaa aatacagtct tttagtttgc ttttagtact aggcaacgag
    15001 ctgcagactg tactgaagta cgacaaagcg agttcaaata atgatgaaat tatttgaagt
    15061 tggaaatagg gaaacggagt ttcctcgtac gtcgcagtaa taaaagataa actaaaggac
    15121 tgaaagagaa atagtgctag gtttttatcc tgcactctgg taaatacaac atgaaaaacg
    15181 atattattaa ctatattaaa gaagttggtc ctgtgaccat ggaccagctg gcagatcagt
    15241 ttggagccag ttctgctaag agctttacag acttggtcaa gctggtttct agcatggaag
    15301 gcagtcgtca acttgtcttt gacaatgctg ggcggattac tcttcctgct ccaaaaatct
    15361 ccaagaacaa ggtgacctta cagggtattt tccgtgccca caagtcgggt tttggctttg
    15421 tgaccattga cgaggaggaa gatgatctct ttgtcagccg tgacgatgtt aactttgcta
    15481 tcgaaggtga cagagttgaa attgcaatca aaaaggttgc agatcgtctc aagggaactg
    15541 ccgcggaagc ggaagtcatt gatattttgg aacatagcct gaaaacagcg gtgggcttga
    15601 ttgtctttga cgaagataag ccagaatatg caggctacat caagtctaaa aaccagaaaa
    15661 ttgctcagaa gatttacatc aagaagtcac ctctggtatt gaccggaacg gaaatcctaa
    15721 aagttgatat cgaagcttat ccaaacaaga aacgggacca ctttgttgcg accattcggg
    15781 acgtggtggg ccacaaggac gatgtgggca tcgatgtctt ggaagttttg gagtccatgg
    15841 atatcgtgtc ggagtttcca gatgaagtct tggcagaggc caatcgggta ccagaaagtc
    15901 cgtctgaaca ggactttgca ggacgtttgg acctgcgaga tgaaattatc ttcaccatag
    15961 acggtgcgga tgccaaggac ttggacgatg cggtccatat caagcagctt aagaacggca
    16021 atctggagct aggtgtccat atcgctgatg tcagctacta tgtgacggaa ggctctgcct
    16081 tggatcaaga ggccatcaag cgtggaacgt ctgtctatgt gaccgaccgt gtggtgccaa
    16141 tgttgccaga acgtttgtca aacggcatct gctctcttaa tccaaacgtg gaccgtctaa
    16201 cccagtcggc tattatggaa attgaccgca agggcaaggt tgtcaagcac tggattggcc
    16261 aaacggtcat caaaaccaac ttccgcatga cctattctga tgtcaatgac atgattgcgg
    16321 gaaatcagga aaagctggac aaatacaaga caatcgtgcc aagtgttgag ctcatggtca
    16381 aactccatga aaccctggaa accatgcgct acaagcgtgg ggctctcaac tttgatacag
    16441 ctgaagctaa aatcatcgtc aacaaggatg gtctgccagt cgatatccag ctacgtcagc
    16501 gaggcattgc tgagcggatg attgagtcct tcatgctggt tgccaatgag tgcgttgctg
    16561 agcattttgc caaactggac ctgcctttca tctatcggat ccacgaggag cctaagtcag
    16621 acaagctgca aaagttcatc gactatgcga ccagctttgg tctgacggtc tatggtacgg
    16681 ccagctctat cagccaggac gcccttcagg acctcatgga gcgggtcaag gatgaaccct
    16741 atgcggatgt cctcaacatg atgttgctgc gttctatgca gcaggcacgc tactcggagc
    16801 acaaccacgg ccattacggt ctgggtgccg agttctacac ccactttacc agccctatcc
    16861 gtcgttatcc cgacttgctg gttcaccgca tggtgcggga ctatggtcat aatcctgcgg
    16921 aaaaagcaga gcattttgag caggttatac ctgagcttgc caagtcttcg tccagtttag
    16981 aacgccgtgc cattgaggca gagcgggaag tcgaagccat gaagaaggca gagttcatgc
    17041 aagaatttgt tgggcaagag tttgatggtg tcgtatctag cgtagtcaag tttggtcttt
    17101 ttgtagagtt accaaacacg gttgaaggcc tgattcatat caccaacctc aacgaatact
    17161 atcagttcca cgagcgaact ctgaccttgc agggtgagaa atcaggtcgg gtcttccgtg
    17221 tcggtcagcc aattcgtatc aagctaacgc gggcggacaa gatgacaggg gaaatcgatt
    17281 ttgcccacgt gccgtccgag ctggatattg tcgaaaaagc tctgaaagcc aaacggagaa
    17341 ctgctagtca ctctaaccgt gaccgtgatg atcggtcagg gcgaggacgg ggcaagcacc
    17401 acaaacaaga agctgctggt cgcgacagcg gacgtcacaa gtctggaaaa gaccacaagt
    17461 caaaaagtgg caaaaaagac aagaaaaaga aaccatttta caaagaagtg gctaaaaaga
    17521 acaagaagaa aaggagatag gcatggccaa gggtgagggc aatgtgattg cgcaaaataa
    17581 aaaagcccgc cacgactata ccattgtcga tacagtggag gcggggatgg tcctgacggg
    17641 gacggagatt aagtctattc gtgcggggcg gatcaacctt aaggacggct ttgcccagat
    17701 caagcagggc gaggcttggt tggtcaatgt ccatatcgct ccctacgatg agggcaatat
    17761 ctggaatcag gagccaacgc ggacccgcaa gctcctcttg cggaagaagc agattgagtc
    17821 tctgggcaat gaggtcaagg gaacagggat gactctggtg cccctcaagg tctatatcaa
    17881 ggatggcttt gccaaggttt tgattgggct tgccaaaggg aagcacgact acgacaagcg
    17941 ggagtcaatt aaacgccgtg agcaagaccg tgatatcaaa cggaccatga aggctatcaa
    18001 tagtcgatag aggctgggag aaatcctggt cttttttgtt atcggaaaac aaatacccca
    18061 gacagtttcg agactatctg gggcttatta tttatcttaa tctaccgtaa tcatagcttt
    18121 aatcgttttc cggttggcca tgtctttgta ggcttggtcg atattgtcca ggctgtaagt
    18181 ttgggtgaaa actcgacctg ggttgatgtc gcctttgaga acggcatcga gcaaaatttc
    18241 cttatcatag gttgtgacag aagcagagcc accaccgata atgatatttt gagcaaaggt
    18301 tgagccaagg gcgtggttgt tgtagtgtgg tacaccgaca aaaccgatac gaccaccatt
    18361 gtgaagcacg ccgagggctt ggtcaataga ggcttctgta ccaacacatt cgagggcagc
    18421 atcagcgcca cctccaagaa tttcacggac tttggcaatt ccttcctcac cacgttctgc
    18481 tacgatggca gtagcccctg attccaaagc catttgttgg cggtctgtat ggcggctcat
    18541 catgacaatc tgagaagctc cgcgcatttt tgcagcaatt acagcacatt gacccactgc
    18601 accatcaccg atgacaacaa ccttgtcacc aggtttgaca tctgctacac ggtctgcgtg
    18661 gtagccagtt ggcataacat ctgccagagt caagagagat ttgatcatgc cttctgagta
    18721 gtcacttggc tgaccaggaa ttttgaccaa ggcccagttg ccgtagtgga aacgaatgta
    18781 ttgagactgg tagttgttgc cccagttggt gtagcctgga tggttgtcac aagtaccgtc
    18841 aaagccagca cgacaggcat cacagtgtcc gcaaccatgt gtaaatggaa cgataacgaa
    18901 gtcaccagga acaagtgttg tgatgtcttc gcctacctct tcaacgatac cgatggcttc
    18961 atgaccagag tttgtgtcac cgtggtcatg gtgaatgtct tctccgtagc cccaaaggtc
    19021 cgatccacag acacaggccc gaacgacctt gataatgaca tcatctttgg cttgaaggct
    19081 tggtttttcg atttctgcaa cggtcatctt accggatttt tcataaatag ctgttttcat
    19141 attcatcaca catttacttg tcagtaaata tcctttctga ttaatatgga atattataca
    19201 ccttggagtt cactccaagt caagaaaaaa atgggtttga attcatattt cctttgtgat
    19261 agactaagtg tatcggaaag aaaaggagag ataatgactg ttttagttcc ttacaaacgc
    19321 atgcccattt ggaatcagga tacggttccc cagcattttt tgaccaagca caataccaag
    19381 gtgggtactt gggccaaaat caaggtcttg agaggtcaac ttaaatttga gccgatttca
    19441 gatgataatg agattttggg agaatatgtc tatggtccga cagccgatat tccctttgtc
    19501 gagccacagg cttggcatcg ggtgactttg ctgacagagg atacggagtt tttcttggaa
    19561 tttttctgtc aaccagaaga ctattttgcc aaaaagtatg actatactcg gacacattcg
    19621 gaagtcttgg aggccttgaa gagaattgag ccttgtaagg tcttggacct gggctgtggt
    19681 catggtagga acagtctcta cttggctcag cagggatttg aggtgacggc ggtcgataag
    19741 gatgtgccaa gtattcggac actcttgcag gtcaaggaag tggaggactt ggacttgcag
    19801 gctgggacct atgacatcaa ttcagccagt ctggagcagg actacgactg gattatctcg
    19861 actgtggtct tcatgttttt ggagcgggat agggtaccag ccattatccg caatatgcag
    19921 gagcagacac gacctggtgg ctataacttg attgtggcgg ccatggatac ggaagatgcg
    19981 ccctgtccta tgaatttctc ctttactttt ggagtaggtg agttgaagga atactaccgt
    20041 gactgggaac tggtcaaata caatgaagat ttcggtcaac ttcacaagcg ggatgaaaat
    20101 ggccatttcc tcaagatgcg gtttgcgacc atgctggcta ggaaaaaagg ataaatattt
    20161 ttttgaaagc acttgcaagg caggtgcttt tgcgttacaa tgtaggtgca aatgggtaaa
    20221 tcatattata ctccacgaaa atcaccttct ggttggataa gagacttgtg taaaatagtc
    20281 tctgagttta tatctctgga gggcgcctac ttctgatttt cacagagtat caggaaagga
    20341 gaagtctatg aaaaagactt ttgttcgtat agccacattt ctaatgttgg tttgcctatt
    20401 ccctgttcaa cttgttcaag cctgttcagg ctttattatt gggaaagggt tgacaacgga
    20461 tggttctatc ctttatggta gaacggaaga ttatccctat ccaccagata atggagcgca
    20521 caataaaaat tatattgtgg ttccagcagc agcctatgcc aagggggata tgttggtgga
    20581 tgaatcattc ggtttcacag caccacactt ggcaaatgag ttcaagtaca cctctacgcc
    20641 tgatgcggca cgtggggatg ggtcaaacgg taattttggt gcgcatggat ttaatgaaaa
    20701 aggtgtgtcc atgactgcta ctgttacagc tattccaaat aaaaaagttt tagctgttga
    20761 cccattggta actgcggggg gactaggtga ggccattttg attgactatg ttcttcctag
    20821 agtcaccagt gcgcgtgaag gtatcgaatt aattgcgaag acaattgatg aaaaaggttc
    20881 ggcagaaggg aatatcattg ttattgctga taagaatgaa gtttggtaca tggaaatttt
    20941 gtcaggccac caatacgtag ccatcaagtt ccctgaagac aagtatgcaa tttttgcaaa
    21001 tacttactat ctaggtcatg ttgattttac cgatacagaa aatgtgattg cttctgcaaa
    21061 ggttgaagaa gtagccaaac aggctgaaaa ttatatgatg gttgatggga agttccatat
    21121 tgccaaatca tatggacctg agaattatgc tgatggtgat cgttctcgga catacgctgg
    21181 tataaaattg ttagatccag catcgtctgt aacctatgaa gatgctgtgt atgatttgct
    21241 acgtcagcca acagacccaa gtcgtcgctt tagtttacaa gatacctttg ccttacaacg
    21301 taaccgtttt gaacatcttc cagaatttcg tccagacgat gaagcaggta aggtaaaaca
    21361 aggggataat ggtgcgaatg accaagcggc agatgcgact tataaatacg ctcttggtaa
    21421 tgaaaacgtc atcgatgccc atgtttatca gattaatagt agcttaccat cagccttcgg
    21481 tggcacagta tggttgggct tggctcagac aaggaataca ccgtatgttc cattttatgg
    21541 tattgtgact gatacttatg aagcctttaa aaatcgctca gcttcttatg ataccaattc
    21601 atggtactgg acggtacaaa atattgataa gatggccatc tcacacccag agttatttgg
    21661 tacatcaatt caagaaaaat ggattgcact tgaaaaagaa tggattgctt cacaggcagc
    21721 tcttgatgct caatatgctg gactttcaga agatgcagcc gtagctctgg ctccaactgt
    21781 aacagaggca accttggctc gttcagcaga gatttttgcc caactaaaag ctgttgaagc
    21841 tgaaatgatg gcaaaaattg aagcagcaac aactccgtca tcaagtagta ctgaaccatc
    21901 gaccagcact gagccatcat caagtggtac cgaaacttca accagtacaa gtcagtctac
    21961 aagtgattct aacacaggag gagctacgga tacttcctca tcaagcggaa cagtagtgcc
    22021 ttctgataag aaagtaacac ctaccaataa aaaaggcaaa tcaaatcttc ctagtacagg
    22081 tgagcaagtc agcatcttgt tggttgcgct aggtgttgca ggaattttaa cagcaatttt
    22141 tcttcatcgg aaaaaatcca gtaaagaata aatgaatatg taaaagtagg ttggattgat
    22201 ttcaatctac ttttttgaaa caattttgtt tacaaatgta gtcatatgtt gtataatttg
    22261 tttacagatg tagtcgaaag aggagtaacc tatgaaacaa gcaagctatc ccattcaagg
    22321 tatgacctgc gcttcctgtg ctctgacggt tgagaaagcg gttggtaaac taatagggat
    22381 tgaagaagcc agtgtcaacc tagcgactga aaaattgagt gttagctatg atgaaaagct
    22441 tttgggacta gaggatattc gtcaggcagt tgaaaaggcg ggttatcagt tagtcgataa
    22501 cttggtgacc gagtcttatg acatttcagg catgacctgt gcttcctgtg ctctgacggt
    22561 tgaaaaagct ctgggcaaac tagagggtgt agaagaagtc tctgtcaatc tggcaacaga
    22621 aaaggcgact attcgttata gccgtcatag gcaaaatcca gctagtcttg aaagagcagt
    22681 tgagcaggca ggttatcagt tgattagacc agaaaaagta gaaggggctg cggacaaggg
    22741 accgagcaag gaagaaaagc tgtggcatcg atttgtctgg tcagcagcat ttactcttcc
    22801 actgctctat attgctatgg gtcctatgtt gccttgggga gggcttccat taccagcttt
    22861 gctccaccaa ccgctagtct atgcgatcag ccaagtcatc ttgctcatcc ctatcctcta
    22921 tatcggacgc agttttttcc aaaaaggctt taaaactctc ttgcaaggcc atccaaatat
    22981 ggattccttg attgctgttg ggacaggggc agccttggtt caaggtcttt tgatgattgc
    23041 gtttcttctg atggggaaag aagtggccat gcacggtcac catccagaac tgtattttga
    23101 atcagccgcc gttatcttga ccttgattac cctaggaaaa tattttgaag ctagagctaa
    23161 aggtcagact tctgaggcca ttaagaaatt gatgaatctg gctcccaaga cggcccaggt
    23221 cttgcgaaat ggtcaggaaa tacaagtgcc gattgaagaa gtggtggtcg gtgaccaggt
    23281 cgttgttcgt ccaggtcagc agattcctgt tgatggtcag gttctcgaag gccagacacg
    23341 tgtggatgag tctatgctga cgggtgagag cctaccagtg aagaaagctc taggcgataa
    23401 tgtttttggc ggcaccctta accagcaagg cgccattacc atgcaggcta ccaaggttgg
    23461 tcgtgatacg actctggccc agattattcg cttggtagaa gaggctcaag gttctaaggc
    23521 tcccatcgcc aaattggctg accaagtatc tgccatattt gtaccagtcg tcatgggctt
    23581 ggctctcttg tccggtctgg cctggtactt cttgggtcaa gaatcatgga ttttttcatt
    23641 aagcattatc atcgccgtct tggtcatcgc ctgcccttgt gctctgggct tagcaacgcc
    23701 gactgccatc atggttggta ctggaaaagg tgcagaaaat ggccttctgt tcaagtctgg
    23761 tcaagctatt gaaaccctac aaggtgtgaa caccattgtc ttcgacaaga ctgggaccat
    23821 tactgagggc aaaccccagg tgacagacgt ccacctcttg tctactaaaa atcgtgagca
    23881 ggtcctccag ctggccgcaa gtagcgagca attttccgag catcccttgg ctcaggctct
    23941 cctgcaagca gctcagacag aaaagattga tctcttgccc gccacagatt ttcaggcact
    24001 ttctggtcgt ggtctttcgg tgataatagc agagcagacc atctatctgg gaaatgaacg
    24061 gttgatgcgg gaacagggaa ttgatgtttc caaaggacgt gcagttgcgg aggcatttgc
    24121 ccagcaagcc aagacgcctg ttttcctagc cagccagcaa gaagtactag cagtcattgc
    24181 cattgctgat aaaataaaag agaccagccg tcaggctgtc caggctctgc aaaccatagg
    24241 actagaggtt gtcatgctga caggagataa tgaaaaaact gccaaagcca ttgccaagga
    24301 agtcggcatt gaacaggtga tcagtcaggt cctaccagat gacaaggcta accaagtcaa
    24361 gcacttacaa gaacagggca agacagtcgc tatggtgggg gatggcatca acgatgcccc
    24421 agctctagct caagcccatg tgggtctagc cattggttca gggactgata ttgccattga
    24481 gtctgccgac attgtcctca tgcatagtga tattttggat gtggttaagg ctgtcaaact
    24541 cagccaggca accatgcgga ccatcaaaca aaatctcttc tgggcctttg cttacaatgt
    24601 gatcggtatt ccagttgcta tgggtctgtt acatgtattt ggtggacctc tgctcaatcc
    24661 tatgtttgca ggtgcagcca tggccctcag ctcagtgtca gtcgtcttga acgctctgcg
    24721 attgaaaacc tacaaactat agcaaaaata ccagtcaaaa aactggtatt tttttacata
    24781 aacgcttaca atgtatatat taagtgcaag ggagatgttt atgacaacct ttattggaaa
    24841 accggtgacc ttggtaggtc cgcagttacg agtgggagat gtagcccctg attttgtcct
    24901 catggccaat gacttgagct tgaaaagcct gaaagacttt ggcaagcaga ctaaggtcat
    24961 cagtgttgtg ccttctattg atacagggat ttgcgatacc caaactcgtc gcttcaatca
    25021 agaattggct gaccgtgaca atacggttgt tattacggtt tcagcagacc tcccctttgc
    25081 ccaaaaacgc tggtgtggcg ctgcaggttt ggaagatgcc atcaccctgt ctgattacta
    25141 tgaccatgcc tttggtaaat cctacggtgt gctcatgcgg gagtggaatt tgctggcgcg
    25201 tgcggtcttt gtcctcaatg ccgacaataa aatcacctac gtagaatact tggataatgt
    25261 caacgagcat ccagactatg atgcggcttt ggaggctgtt aaataaagat ataacggtgc
    25321 cggaacaggc atcgtttttt gatggaatat ttttgtctaa tttgatatac taaatttatc
    25381 aaaaacaagg agggtctttt atggaaaaga cacagattat tcaagatgtt ttggccctca
    25441 ttcaagactt gaatcgggcc tttcaagcag ataaagtagg aacagctgat gaacaacggt
    25501 tccaacataa tctgacagag acgacacgca tattgtcgga ggccgagtct gttaccaata
    25561 cagaactgat tgcgattgag aaattttatc gttcgaccag ttttttggtt ggactgggcg
    25621 acctgcgatt gagtgaaacc agcaggcagg cttggcgggc atttgaccga tattactacg
    25681 aaacgatcag agaagaactc aaactctatg gcggctctgc tattgcccaa gtgtaaaaga
    25741 tgaaggagag ggtgaaaacc ctcttttgtg ctataataag tagtaagaat acagttgtta
    25801 ggaagagaag aagcgaggtg tatcatgtcc tatatccaag taaagaattc gtcaaaatac
    25861 taccaaatgg gggatgcgac cattgtggcc aatgatggtg tgtcctttga gattgagcag
    25921 ggtgagctgg tcatcattct gggttcatct ggtgcgggca aatccactct gctcaatatc
    25981 ttaggtggca tggatagcaa cgatgagggt gaggtttgga ttgacggtgt ggatattgcc
    26041 aagctttcaa gtcatgaatt gaccaattat cgtagagatg atgtaggttt tgtatttcaa
    26101 ttttataact tggttgccaa tctgaccgcc aaggaaaatg tggaactggc agcagaaatc
    26161 gtcaaggatg ccttggatgc cgaggaagtc ttggaacagg tcggactcgg tcaccgaatc
    26221 aataatttcc ctgctcagct ttcaggcggt gaacaacaac gtgtggccat tgcccgtgcg
    26281 gttgcaaaaa agcctaaaat tttgctttgc gatgaaccaa ctggcgctct ggactaccaa
    26341 acaggtaagc aggtcttaca aatcctacaa gatatgtccc gcacacaagg gacaacggtg
    26401 attatcgtga cccacaatag ctctctggct cctattgcag atagggtcat tcggatgcgg
    26461 gacgcccgtg tgcactctgt tgagttacac gcagaaccac aggatattgc aagcctagaa
    26521 tattagtggg tgaaactatg aaaaagaaaa tctactggaa ggatatgaga caatctctgc
    26581 tttcttctaa ggggcgtttt ttgtccatat ttagcctgat gatgctagga gctctagctt
    26641 tgacaggtct gaaagtaaca gctcccaata tggaaaagac ggcccaagcc tatatagcca
    26701 gtcatcgaac gatggatttg gcagtaattt caggtctggg acttagtcaa gctgaccttg
    26761 acgaattaga aacgatagaa ggtgctactc ttgaagcggg ctactttaaa gatgtggtta
    26821 ctaatgaagg tcagactgca attcggctgt tttcagctcc ccagacccta tcgacctata
    26881 agctagtaga aggggaaatg cccagtcaaa aggggcaaat agccctatca gcttctttca
    26941 aaaatcgtta caaattggac gatactgttc aagtgacgga gtctgataag gatggaaaaa
    27001 tcttgacaca gacaagtttt actcttgtgg gctttgtcgc atcagcagaa atttgggaca
    27061 atgaaaccat gggcatggct gcaagtggtg atgggcagtt aggtggctat ggccttgtta
    27121 gtcaggatac cttcaaatca gaagtttaca ctattgcccg tattcgctat gatgatttgg
    27181 ttgatttacc atattacagt caggcctatc aagacaggtt ggaccagcac caagaagacg
    27241 tagaaaagtt attggctaac aatgatgaac aacgattggc agctattcag tcagatggac
    27301 agacagatat ttcaaaaggg gaagaggaaa ttgctcaggc tcaatctcag cttgaacagg
    27361 cagagtcaga actggaaaca ggggaggagc aattagcgac aggtcgtaga gagtttgcgc
    27421 gtggtagggc taaggttgtc caaacggaag ctgaattacg agctgcccaa gcccaactat
    27481 taaaaaataa gtttgaattg gaagaaagta aaaaggaatt ggacgggcgt aaggctcaat
    27541 tagaacagac tgcttcgttt ctagacggct atcagatgga attagaaacg tcagcccagc
    27601 agttagaaga ggccaagcaa gaattggatt cccaaaatgc ccaactgagt cagaccgcat
    27661 ctcagatttc cacagggcga gctagttggt ttcaagctca gcaagagtta gaccttgaaa
    27721 ttgccaatca cttgcaagaa ggccaaaccc tctcagacta tcctgacttg ttggctagac
    27781 aggaaggttt ggatgttgag aaaagccgat tagaccaaat ggaatctgca catgagcagg
    27841 ccaaccaagc ctatttacaa ggttatgatt actatcaaac caaacaaaat gaatacaata
    27901 gcaatctagc ccagtaccaa acttggaatc aagaatatca ggcaggtttg gcccgttatc
    27961 aagcaggttt gagtcagtat gaacaaggca ttgcagccta caataaagga gtagaagact
    28021 atgaatgggg actaagtcag ttagagtctt caaatcaact gctccgtcag gaagaattcc
    28081 gtttggagga ggctgataaa gagttaagtc aagctcaatc ccaattttct gagaagaaag
    28141 ccacggccga tcaagaaatt agccaagctc aaaccgagat tgctcaggca aaatccgact
    28201 taagcaagct ggaaaaagcg ccctatcagg tctatactcg atcaagccta ccaggtggag
    28261 atggctatac tacctatagc aatgcgacta ggtctattgc tgcagtgggc aatgtcttcc
    28321 cagttgttct ctaccttgtc gcagctctag tgacttttac caccatggcc cgttttgtcg
    28381 atgaagagcg aacacagtca ggtctcttca aggcccttgg ctatacgaat agacagatta
    28441 tggctaagtt cattctctat ggtttgacgg caggtctggt aggaacaata gtcggtataa
    28501 tagcaggcaa tctcctcctg tctccactta tttccaacat tattacgcag acaaccgtga
    28561 ttggaccagc taaacttcat ttttatcccc tttggacagg tttagccttg ctcctgtctt
    28621 tggcttcctc ggtcctgcca gcttacttgg tggctcgtcg ggagctgaca gaaaaacctg
    28681 ctcagctact tttacctaag ccacctgtta caggctccag catttggctt gaaaaatggc
    28741 cggctatctg gtcccgcctg agcttcaccc ataaggtgac agcccgcaat atcttccgct
    28801 acaagcttcg tatgcttatg accatctttg gagtagcagg gaccgtcgcc cttctttttg
    28861 gaggactggg tatccgttcc tccatctcag gagttgtcca acgacagttt ggggagctga
    28921 tccattatga tatgttggtg gtggaaaaca gcagagctac tgaggaagag ttggataagt
    28981 taacacattt tcttcagtca gatcaggttc gccaatcctt gccagtggct tttgaacagc
    29041 tcaatcaaac ggtggaagaa gacggtcaga gaaagaattt atcgattagt ctctacattt
    29101 cagaccgtcg ggattttgga aatcaagtca gtctggaaag ttcgacaggt cagcctatta
    29161 aactgagtga cagggggatt gtcctcacag aaaaattggc gcaaatctac ggggtctcag
    29221 ttggtgataa gttatcactg accttggaag ataaggaagt ttcagtcagg gtagaggctg
    29281 tggctgatat gtacgcaggg cattttatct atatgacaga tagctactac gaacaagtga
    29341 ccggcaagca gaagactgcc aatgcctatt tggttcagtt gaaagacagt cagttggggc
    29401 atattcagac tcttgcaagt caactcctag cgatgccagc agtcagaagt ttggttcaaa
    29461 atacttctct tatagatatg cttaccacga ttgcaggctc gcttcagacc atcatgacca
    29521 ttctagtcat cctatccatc ttgcttggct tggttattct ttacaacctt acaattatca
    29581 atatgtctga acggattcgt gaattatcca ccatcaaggt actgggcttc cacaataaag
    29641 aggtgaccat gtacatctat cgcgaaacca ttgtcctttc gctgattggc atgttggttg
    29701 gactggttgg tggtatttat ctccacaagc tccttcttgc catgattggt tcggacagta
    29761 tccgcttcaa tccatctgtc ggactagaag tctatctcat tcctatctta gccattagtg
    29821 gaattttggc tgttcttggt tggtacgtca atcaccatct gagaaaggtg gatatgctgg
    29881 aagccttgaa gtcagttgat taaaacagtc tgggaataga ctgttttaac ccgagccttg
    29941 aaatcggaga gcgaggcaca gtcttggttt ggaaggaatt ttaaaaagag aatgacagtc
    30001 ttgcgattca aaagaggctg agcaaaaagc ccaggaccac tactcagagt tcgtgtcaac
    30061 atctcagcgc agtggttgat tggcagattt gttcgtgttt tgcactccaa atctggccat
    30121 tacgactgat gcgaacagag ttcgcttcat ttccaacctc caacagtcac tactctgact
    30181 gttggagcta tgcgggggtg ggagtgaaac agtctgggaa tagactgttt cagctcaaca
    30241 actggaaata aggacttgtt gacgaactct ttttgactta gtcgagttct tatacgaaaa
    30301 ctcaaaagaa tgcaaaaact ccctccctat gatataatca aagcgaaaaa ttttttatgt
    30361 taggctacta gctcgtccct agtagtcttt ttctgtgcta aaaattcagg atacgttatc
    30421 tgctctttta agaggatata agctattttt aataattgat gtgcgagtgc aattgttgct
    30481 ttttgtgaac cacgccgact ttgaatctga taaaatcggt ctgctagggg actacctttt
    30541 tgttttttga tagcgaaagc ggcttggcat aaacatttct tcaaatatga atttccgtgt
    30601 cgaatcttgg tacttcgttt tttaccagca ctctcattat tacctggaca aagtcctgcc
    30661 caagaagcta gatgtccagc agttggaaat tgactcatgt caacaccaac ctcagaaatg
    30721 ataacagaag ctgtaatgac atcaatgcct gggatggaat ccaatatttc tacatggttt
    30781 tcatattgtg atagatagac attcattcgc tcttccaaca actcaatctg cttctgataa
    30841 aaatcataaa tctccaagga ctgctttaac ataaagcggt gatggtcaga aaaatagcca
    30901 tccaaggctt ccagaagctg cggcactttc ttcttcaagc tggtataaac agattgatgg
    30961 acaatgcgag gtgtaatagg cgtcccatca accagtagct gaaggagatt gcgaccagat
    31021 agccccataa tatcttcaat ataggttgtt agcttgatac cgcctgactg aagaatttta
    31081 tggatacggt tggtttcgcg attgcgactt tccacataat gttttcgctg tctggtcaat
    31141 tccctcaatt cttgaatagc ttcatcgggg acgaaactcc gaggcaatag accaatccgt
    31201 gttaatttgg caatccagtg agcatccttc ttgtcggttt tctgacctgg aatagccttc
    31261 atgtgggctg gttgagcgag tatcaacttg aagtcatcac atagagcatg ccagacaggt
    31321 cgccaataaa cacctgtgct ttccatacca acagcttcca catgaaattg actgagaaaa
    31381 tcgtggcaag cacgtaggcc tttagtcgtt gtatcaaatg tagccatctc acgctttgga
    31441 cgagttgagg tgagtggtcc atgtaggata cagacgacaa tattggcttg atggacatca
    31501 atacctgcac aagattgata gagaacatcc atgataattc tctccttcta aaaattagag
    31561 agcttatcta ccgaatttta gtcgtatttt tatactcatg cctatcgcat attttggggt
    31621 gctcgtcagt agagtgggtt agtttttccc gcgatgacta acatcagtaa ctcgtcaacc
    31681 acaaagccct cactaagatt atacacctag tgagggcttt atgttcgttt tcattattct
    31741 gtgggtgagg gcggagccat catggatgtc tttcccactc ccttttctgt aaacacaagt
    31801 ctagaagtgt gctataatca tctcatgaaa aaaataagtg atttaggatt aacaggtcga
    31861 aaattagttg gagaaggtct aatcttagtc ttcatcggga ttggattttt aattgctggt
    31921 tggcaatttc caggactgat tcttcgattt gttcacgcag gtctattctt tttagcttta
    31981 tatgaattga gcatgacatt tttcagaaag aagaaatcga gtgaaagtgt cattgcgcta
    32041 gttgggaagg ctgttctttt cgggatccta gccagtctcg atttagctgt tcaaattccc
    32101 ctctatttcg cggctatttt tgttgggatt tatcaacttt ttacagctct tatcaatttc
    32161 attacttttt acctatatcg aaaagatgga gtacagccac ggattcgcta tttaatagat
    32221 ggaatttggt tatccttact aggttcagct tccctatttg tgtcaggcac acagctggtt
    32281 gttcaaacca ttgtcatcgg aggttatctg atcttgtatg gactaactaa tctgcgtgat
    32341 ggcttcttgt ttgaggaggc gattgagcaa cagagtctaa agcgccatgt acgcctaacc
    32401 ctccccttgt tccttgcagc ccttgtacca cggatgaccc tgcaaaaagt caatgattac
    32461 ttggcggata atgagggaca aacggctcaa tctatctata accgccacaa agaaattgcg
    32521 gagtttccag cattagaagt ctttgtacac gttggagaag aagggttcgg agcagttgga
    32581 catgttgatt tgagttacaa agggcaggtc tacggttttg gttcatatga tgtcctgtca
    32641 gagcgtttgg gtggtgcgat tggtgacgga gttttgttta gagctgagcg tcaggcctat
    32701 attgattttt gtaaccaaga aggaatgact atgttgggct atcaattggc attgagttct
    32761 gagcaggaaa aagcggtaga aactcgacta gctgaaatag aaggtttact gcttccatgg
    32821 caaccaagtg cggaaaaggt ctctagacgg tcagacggcc agcctattga aatgtatgcc
    32881 taccgcatga aagaggcaat tggggcagta cttttcaaat ttaaaaaatc aaaattcaaa
    32941 acctactttg tcctctcaac caactgcgtc cttttggctg attcagtaat cggtcaagca
    33001 ggaacagatg tcctaggctt gcgcggtttc attgctccag gaacctacca atcttatctg
    33061 gatcaagaat acgaaaaacc tcatagttta gtagtcgcaa agaacattta ttatcgaaaa
    33121 gagaaatcct agtcactgac tgggattttc tgttattcca gctctctaag ttccacaaaa
    33181 ctcccccatc catgctataa tagagacatg aaaaaaaata aattattatt aattgatggc
    33241 tcttcggtag ccttccgtgc tttctttgca ctttataatc aaattgatcg ttttaagagt
    33301 ccgacaggtc ttcacaccaa tgctatctac ggctttaacc tcatgctgga ccacatgatg
    33361 aagcggattg aaccgaccca cattctcgtg gcttttgatg ctggaaagac caccttccgt
    33421 acggagatgt acgccgatta taaggcaggt cgtgccaaga caccagacga gttccgtgaa
    33481 caattcccct tcatccgtca gatgctggat gctatggggg tcaagcatta tgagctggct
    33541 cagtacgagg cagacgatat tatcggtacc ttggacaaaa tggcagagcg gacagagatt
    33601 cctttcgatg tgaccattgt tagcggtgat aaggacttga tccagctgac agacgaaaac
    33661 accgtagttg agatttctaa gaaaggcgta gccgaattcg aagaatttac tccagcctac
    33721 ctcatggaaa aaatggggat tacccctact cagtttattg acctcaaggc tttgatggga
    33781 gataagtccg ataacattcc tggcgtgacc aaaatcggtg aaaaaacagg tctaaaactc
    33841 ctgacagaat ttggttcttt ggatggtatc tacgaaaaca ttgacagcat gaaggcttct
    33901 aagatgaaag aaaacctgat tgctgataag gagaaggcct tcctatctcg tacacttgct
    33961 accattgata ctcaggcacc aatcgaaatt ggtctggatg acattgttta tcaaggcccc
    34021 aaggtggatg aactaggaca attttatgat gacatgggct tcaaacaatt aagggctcag
    34081 ttaggtacga ccagctcgca agaggaagcg gtactcgatt tccaaatcgt aacggaaatc
    34141 agccccgcta tgctcaaaca agaccagttt ttctattttg aaatcttggg tgaaaattat
    34201 caccgagagg atttggttgg tttagcttgg ggagataagg agaaaatcta tgtcggaggg
    34261 cctgagctac ttgatagtcc agtcttgcgt gactttttag aaaaccaaac tataaaaacc
    34321 tatgacttta agcgtggaaa agttcttctc gaccgaaaag agattacgct acctccagct
    34381 actttcgata gccgtctagc caaatacttg ctttccaccg ttgaggacaa ttccctaaca
    34441 accattgcca acctctatgg tcagatcagt cttgttccag atgaggcagt ttatggcaag
    34501 ggtgctaagt tagacttgcc agaacgcgag atctttttcc cacacttggc acgtaaggtt
    34561 caggttttga ttgagacaga agagccgatg ttgaccaaat tagaagaaaa tcagcaatta
    34621 gatcttttgt ttgacatgga gttgccactg gcaaatgttt tagctaagat ggaaattgct
    34681 ggtattaagg tggaagctga gactctcaaa gctatgcaat ctgaaaacga agttttgatt
    34741 gaccagttga ccaaggaaat ctatgaacta gctggtcagg aatttaatat caattcgcct
    34801 aagcagcttg ggaccattct ctttgaagag atgggactac cgttggaata taccaagaaa
    34861 accaagacag gctattcaac agcagtagat gtcttagaac gattagcgcc aattgcacca
    34921 gttgtttcaa aaatcttgga ataccgtcag attaccaaac ttcaatcaac ttatgtagtc
    34981 ggcttgcagg atgccatttt agaagatggt aagatccata cccgctatgt gcaggatttg
    35041 acacaaacag gtcgtctatc ttcgacggac cctaacttgc aaaatattcc tgttcgtcta
    35101 gaacaagggc gcttgattcg taaggcattt gtgccatcgc ttgaaaatag tgtccttttg
    35161 gcatcggact attcacagat tgaattgcgc gtcttggctc atatttctca ggacaagcat
    35221 ttgattgagg ccttccagca aggtgcagat attcacactt cgacagctat gcgggtcttc
    35281 ggtatcgaaa aagctgagga tgtgacacca aatgaccgtc ggaatgccaa ggctgtaaac
    35341 ttcggtgtcg tctatggtat ttcagatttc ggtctatcca ataacttggg gattacacgt
    35401 aaggaagcta aagcctatat cgatacctat tttgaacgct tcccaggaat taagaattac
    35461 atggaaacca ttgttcgtga ggcacgtgat aagggttatg tagagacgat ttacaaacgc
    35521 cgtcgtgaat taccagatat aaactctcgc aatttcaatg tccgtaattt tgcggagcga
    35581 acagctatca actcaccaat tcaaggttcg gccgcagata ttctcaaagt agccatgatt
    35641 aatcttgaca aggctttgac tgaagcaggt cttgcgacac gtatgttatt gcaagtacac
    35701 gatgagattg ttttggaagt cccagtggca gagctagaaa cggtaaaagc catggtaaaa
    35761 gagaccatgg aatcggcgat tagcctgtct gtgccattga ttgcggatga aaatgaaggt
    35821 tcaacatggt atgaagcaaa gtaagaaagg aaaaagtatg aaaaaattaa ttctttgtat
    35881 tggtctatgt agtcttctac ttgcaggttg cggaaaagca tcgcagaaga ctgagaaaac
    35941 accagcttct tcaggggaag agttttcaac caccttaccg attttgcaag aaaaacagga
    36001 tactacgaat gaacagttca atgttttagc aaatgcagag gttgtagctg ttaatagtac
    36061 accacctgcc gacgaaacac aaaaaggcca caagatttat gcggccaata atggtgttgc
    36121 tgaaaaagat gaaaatggga atatcaagga atatttccgt tattataatg tgccatctac
    36181 ttggacaatt aatgcagaaa atactaagga tgagacggtc gctgcagtct acgatgttaa
    36241 agagggggga agcaattaca tggttcaact ctataatatc aatgctttta atcagtctcc
    36301 attggaagac ggtcgcaata tgacgccgga ggaattagaa gcacggatgg ttgaaacaaa
    36361 tcattctttc aatgaaaaga cggttgtcac aatcaatggt caggaatggc gggttgggcg
    36421 acagttatta acagatcaaa aattagctcg tctgactttt tatcggatgg aatcgacagc
    36481 tgcttatgac gactctgtgg ttgtgggttc aatctattat tccttagatc caggaaccga
    36541 taaagatcgg accaatctta aaaaggttat cgggcaagtt aaggatgtcg tgtaccaaat
    36601 tgctaaaaaa taatcaaggt agacaagcta taatcagtaa cgagttaaca ctgacaagat
    36661 tgtgttatag cacaccttat tcattaagct tttattgatt ctaacattta atatacatta
    36721 aaggaggtct ctatgtctta ttccttccaa aatcctagtc aggacattat ctttgactat
    36781 ctgaaaaatg ctaagaccat tgcagtggtc ggtttgtcta gtcgagaaga gacggcagcc
    36841 taccgcgttt ccaagctcat gcaggaagcg ggctataaaa ttattccagt taatcccaag
    36901 gctgctggtg ggactatttt aggcgagctg gtctatagta gtctagcaga gattgaccaa
    36961 cctattgata ttgtagatgt tttccgcaga agtgaatttt taccagaggt agcacaggaa
    37021 tttatccagt cgaacgccaa aattttctgg gctcaactcg gtttagaaag ccaagaagca
    37081 gaaaaactat tgcgtcaggc aggtcgtaac gatattgtca tgaataaatg tatcaagatt
    37141 gaatatcttg aaatgaaaga acaatactaa taagtcgggt atatttatcc gacttttttc
    37201 atcgaaaaag cagttcaatg aaccattgaa ctgccggatg actctaagga ggagcactta
    37261 gaattggaac aaattactac acatggcgta aaaaatagaa aggaagaaat ctgctccaat
    37321 accaagcagg ctaaaatagt cctttgtcgc tcctcaa
//
LOCUS       DAZQCJ010000008        35928 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000008 DAZQCJ010000000
VERSION     DAZQCJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35928
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.008"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            51..1640
                     /locus_tag="ACHU66_000391"
     CDS             51..1640
                     /locus_tag="ACHU66_000391"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFR3388705.1"
                     /translation="MSKTIQAITNQIWSMANELRGNMDASEYKNYILAFMFYRYLSEH
                     QERFLVEDEIISPAPGETVQDAYAREAVGNDLVEHLENTASHLGYAIEPEDTWARLVA
                     KIDNSEIVASDYQTIFDHFNANVELNRDAMEDFRGVFNDINLGDSRLGNSTVARAKSL
                     NSIVKLIDSIEYKNDEGKDILGEIYEYLIGQFAASAGKKGGEFYTPHQVSKILAKIVT
                     LGLEKSDTSFSVYDPTMGSGSLLLTVRNELPQGQHIKFYGQEMNTTTYNLARMNLMMH
                     QVSYSNMILNNADTLESDWPDGVDELGIDQPRSFDAVVANPPYSAKWDNRESKLKDPR
                     FMEYGKLAPASKADFAFILHSLYHLNNTGTMAIVLPHGVLFRGAAEGHIRKLIIEKNY
                     LDAVIGLPANLFYGTGIPTTILVFKKNRQTKDVFFIDASKEFEKGKNQNHLSDDMVEK
                     IVETYHNRQSVDKYAHLASIEEIVENDYNLNIPRYVDTFEEEEEIDLGQVTQQLEQDR
                     LEIRALEDKIRQQLKTLGVDF"
     gene            complement(1691..2965)
                     /gene="serS"
                     /locus_tag="ACHU66_000392"
     CDS             complement(1691..2965)
                     /gene="serS"
                     /locus_tag="ACHU66_000392"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR3388706.1"
                     /translation="MLDIKRIRTDFDGVAAKLATRGVAAETLANIKELDAKRREILVT
                     VEELKAERNTVSAEIAQAKRNKENADDKIAAMQKLSADVKNLDAQLLEIDEELNAIIT
                     VLPNTPHDSVPVGADEEENVEVRRWGTPREFTFEPKAHWDLGEDLDILDWERGAKVTG
                     ARFLFYKNLGARLERALYNFMLDEHIAEGYQEIITPYMVNHDSMFGTGQYPKFKEDTF
                     ELADTNFVLIPTAEVPLTNYYRNEILEEKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFATPEQSYDELEKMTANAENILQKLGLPYRVLALCTGDMGFSAAKTY
                     DLEVWIPAQNAYREISSCSNTEDFQARRAQIRYRDAADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGGVEVIKP"
     gene            complement(3183..3554)
                     /locus_tag="ACHU66_000393"
     CDS             complement(3183..3554)
                     /locus_tag="ACHU66_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFR3388707.1"
                     /translation="MAQSQNTSVELQTTGVSYMGFGGKVGKFLIGDKALEFYPDSNVE
                     RYIQIPWSEMTSIGANVSGKAISRHFEIYTEKSRFLFASKDSGKILKIAREHIGNEKV
                     VKLPTLMQTIGRKISNLFAKK"
     gene            complement(3614..4525)
                     /locus_tag="ACHU66_000394"
     CDS             complement(3614..4525)
                     /locus_tag="ACHU66_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR3388708.1"
                     /translation="MSEKIQLSVSDRKKVWWRSTFLQGSWNYERMQNLGWAYAMIPAI
                     KKLYTKKEDQAAALERHLEFFNTHPYVASPILGVTLALEEERANGAEIDDTAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLALSGNIMGPIIFFLGWNIIRMAFLWYTQE
                     FGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVSITFAVDLPAKQLSEGA
                     YIVFPEGTVTGAELKGILGQALSGLSLEPTQAQTLQGQLDSLIPGLMGLLLTFLCMHL
                     LKKKVTPITMIIGLFIVGILARFFGIM"
     gene            complement(4543..5352)
                     /locus_tag="ACHU66_000395"
     CDS             complement(4543..5352)
                     /locus_tag="ACHU66_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR3388709.1"
                     /translation="MSDITLISAILVVIVAFFAGLEGILDEFQFHQPLVACTLIGLVT
                     GNLAAGVMLGGSLQMIALGWANIGAAVAPDAALASVAAAIIMIKGGDFSTEGIAVATT
                     TAIPLAVAGLFLTMIVRTISVGLVHGADNAAKDANFAAVERYHLFALLLQGLRIAVPA
                     ALLLAIPAEAVQAVLESMPAWLSGGMAVGGGMVVAVGYAMVINMMATREVWPFFALGF
                     AFAAISKLTLIALGVIGVAIALIYLNLSKQGGSGNGGASSNDPIGDILEDY"
     gene            complement(5384..6376)
                     /locus_tag="ACHU66_000396"
     CDS             complement(5384..6376)
                     /locus_tag="ACHU66_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR3388710.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPSEGPDD
                     LYAKLNAAVDAFDADDEVLVLADLWSGSPFNQASRVAGENPDRKFAIITGLNLPMLIQ
                     AYTERMINAAAGVEEVAANIIKEAKDGIKVLPEELQPAEATPVADAAPVALQGAIPPG
                     TVIGDGKLKINLARIDTRLLHGQVATGWTPASKADRIIVVSDTVAKDELRKQLIKQAA
                     PGNVKANVVPIKKLIEVSKDPRFGNTHALILFETPQEALEAIEGGVEIKELNVGSMAH
                     STGKTMVNNVLSMDKDDVATFEKLRDLGVEFDVRKVPNDSKKDLFELINKANVQ"
     gene            complement(6598..7410)
                     /locus_tag="ACHU66_000397"
     CDS             complement(6598..7410)
                     /locus_tag="ACHU66_000397"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFR3388711.1"
                     /translation="MKKKIIAIDLDGTLLNNDSQLSDYTISTIKKVRQAGHTVLIATG
                     RPYRMAEKFYQQLELDTPMINFNGSLVHIPGKKWQWEQNILIDKKYLLEFLKEEETFE
                     ADFIAGEYKNKFYITQKNLDKIDPALMGVEQITPDTLIKPELITSDPHSILMQTRATD
                     KYELAKEMNAYFKDELEINTWGGPLNILETCAKGVNKATALSYVLNLYQATPSDLIAF
                     GDEHNDVEMLDLAGTAYAMKNCSDTLRPHADRLTAFANFEDGVARELENLFL"
     gene            7756..9215
                     /locus_tag="ACHU66_000398"
                     /pseudo
     CDS             7756..9215
                     /locus_tag="ACHU66_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
     gene            complement(9563..10423)
                     /gene="metF"
                     /locus_tag="ACHU66_000399"
     CDS             complement(9563..10423)
                     /gene="metF"
                     /locus_tag="ACHU66_000399"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HFR3388712.1"
                     /translation="MIGQTPSLSFEIFPPKPEVGNEKIIQTLDEMQGLAPHFISVTCS
                     NNNLNVEETTVKLANHVRNELHIPTIAHLPAAYLTKEKVRSVLHSLDEIGVHQILALR
                     GDIIEGLPPKEDFQYATDLISFIKEEAPHFDIIGACYPEVHPESPNSVSDIKNLKKKV
                     DAGCSTLVTQLFFDNEAFYNFQEKCSLADIEVPIIAGIMPIINRNQALRLLKTCENIR
                     LPRKFKAILEKYEHNPESLRAAGLAYAVDQIVDLVTNDVAGIHLYTMNNAETARTIHQ
                     ATHSLFKHYS"
     gene            complement(10427..12676)
                     /gene="metE"
                     /locus_tag="ACHU66_000400"
     CDS             complement(10427..12676)
                     /gene="metE"
                     /locus_tag="ACHU66_000400"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR3388713.1"
                     /translation="MTTTIIGFPRIGEHRELKFITEKYFRNEIPQEELLEVAKDLRAK
                     HWNIVKEAGITEIPSNDFSHYDNVLDAAVLFNIVPKAVQNLDLTDLEKYFALARGYQG
                     EKGDVRARPMKKWFHTNYHYIVPAIEKDTEIKLAGHKIFDEFQEAKNLGITTRPVLIG
                     PFTLLQLTDFEEGLTATDFADSLVAAYGQVFEKLAELGAEKIQLDEPSLVKDLTAEEK
                     ALFLRIYQTLLADKKGLQVLIQTYFGDVRDIYTELTSLPVDAIGLDFVEGKETAALVA
                     TGFPANKTLYAGIVNGKNIWRNNYEKSLAVLDAIPAEHVVITTSCSLLHVPFTTANEE
                     FEPAILNHFAFAVEKLSELRDLDAIRNSQGEVALTANKELFALERVGRDAALADRLAG
                     LTDADYTRLPVFAEREAIQREKLNLPLLPTTTIGSFPQTKEVRSTRLAFRKGNISEEE
                     YDTFVKAQTDEWIAWQEEVDFDVLVHGEFERNDMVEYFGENLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKE
                     STLQIALAIKEEVLDLEAAGIKIIQIDEAALREKLPLRRSDWYSEYLDWAIPAFRLVH
                     STVAPETQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLVILDELKAKNFQTQVG
                     PGVYDIHSPRVPAVDEIAHTIQAILAKVPKEKVWINPDCGLKTRGESETKASLIHLTQ
                     AAKTARKEL"
     gene            13056..13745
                     /locus_tag="ACHU66_000401"
     CDS             13056..13745
                     /locus_tag="ACHU66_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFR3388714.1"
                     /translation="MGKVIIGISGNEQEFPTKSGRTYVTVASELADGVRQAGGVPMVI
                     PMGTPDLAKDYINMIDKLILSGGQHVDPSLYGQERLIDSNDYLLERDAFELALIEEAL
                     RQGKPIFAVCRGMQLLNVALGGSLEQEVAHHWQEGIAGTSHRLQVKPKSRIGQLFTQG
                     SPINSFHQQRIKELAPGLVATGLDPRDGTIEAYESRGNQALFGIQWHSEFLYNDCKQH
                     RDLFQYLVDIF"
     gene            13897..14748
                     /locus_tag="ACHU66_000402"
     CDS             13897..14748
                     /locus_tag="ACHU66_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR3388715.1"
                     /translation="MKLKKLFSLATAALSVGVLVACGNSSSSSDSSATTVRVGVMSLS
                     DSEQARWDKVQEILGDEVKLEFTQFTDYSQPNKAVAENEVDINAFQHYNFLNNWNQEN
                     GEDLVAIADTYIAPIRLYSGTADGKNKYTKVEEIPDGAEIAVPNDPTNESRALYLLQA
                     AGLIKVGVSGTELATIADITENKKNLKITELDASQTASSLSSVDAAVVNNTFVLEAGL
                     DYKNALYKEQKDENSKQWYNLIAARSDWEKSEQAAAIKKIIEAYHTDEVKKVIEETSD
                     GMDEPVW"
     gene            14905..16272
                     /locus_tag="ACHU66_000403"
     CDS             14905..16272
                     /locus_tag="ACHU66_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFR3388716.1"
                     /translation="MADSKVQAFENDAIIQNYFEKLKVLISKKSIFAQQIGLLDVATY
                     LKDMFEEAGADVVLDDSYAAPFVMATFKASVPDAKTLIFYNHYDTVPADADQVWEKGN
                     PFELTISDGYIYGRGVDDDKGHITARLSALKKYQARQNGDLPVNVIFIMEGAEESASV
                     DLDKYLSKYKEYLIGADLLVWEQGHRNSLHQLEIAGGNKGIVTFDLQVKSADLDIHSS
                     YGGVIDSASWYLLSALQSMRAADGEILVDGIYEQVQEPNERELALVEEFALATSQSMT
                     DIYGLTLPTLVEDRREFLKRLYFEPSITIEGLSTGYLGQGVKTIIPAQASAKMEVRLV
                     PGLEPHDVLDKIRQHLDKHGFDKVELVFTLGEMSYRSDMSHPAIVNVIELAKKLTPEG
                     VAVLPTSPGTGPMHTVFHALGVPIAGFGLGNANSRDHAGDENVSIADYYSHVELVEEL
                     IASYE"
     gene            16265..17329
                     /locus_tag="ACHU66_000404"
     CDS             16265..17329
                     /locus_tag="ACHU66_000404"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFR3388717.1"
                     /translation="MSKEMIKLDNIDVTFQQKKRTIEAVKDVTIHINQGDIYGIVGYS
                     GAGKSTLVRVINLLQVPTAGKITIDEDVIYEGEKVTLTPTQLRSKRREIGMIFQHFNL
                     MAQMTAEENVAFALKHSGLSKEEKKEKVAKLLDLVGLSDRAENYPAQLSGGQKQRVAI
                     ARALANDPKILISDESTSALDPKTTKQILALLQELNEKLGLTIVMITHEMQIVKDICN
                     RVAVMQDGRLIEEGSVLEIFSHPKEELTQDFIKTATGIDEALVKIYQQDIVKNLPENS
                     ILVQLKYAGSNTDTAIVNDLYKFYQVSANILYGNIEILDHTPVGEMVVILSGEPGQLH
                     RAIEAVTEARVEVTILKGAN"
     gene            17330..18022
                     /locus_tag="ACHU66_000405"
     CDS             17330..18022
                     /locus_tag="ACHU66_000405"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFR3388718.1"
                     /translation="MLEWIQTNFPDIYKLGWDGQTGWLTHFNLTLYMTFVSFAFGGFM
                     GLVSGLFLVLTGPRGVIANKTAYWILDKVASIFRAIPFIILLAAIAPFTKIIVGKTIG
                     TEAALVPLALSVFPFFARQVEVVLSELDRGVIEAAQASGATFWDIVLVYLREGLPDLI
                     RVTTFALVSLVGYTAMAGAIGAGGLGQVALSYGYLRYNNDVTFLATLLILVIIFVIQF
                     IGDFLTRKISHR"
     gene            18204..18908
                     /locus_tag="ACHU66_000406"
     CDS             18204..18908
                     /locus_tag="ACHU66_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HFR3388719.1"
                     /translation="MSQFVVDSSFWSLFPDAKIGVLLLKDYKTPAQSPDELVKLLEES
                     NGIAQKFLTEDTFSENEVIRTYRQAYQKFKTKKGARSSIEALLKRSTSDRPVTTISPL
                     VDIYNAASLRFGLPCGAEDLDTFVGNLQLTVTEGGDEFYLIGDETNNPTLPGEVCYKD
                     DKGAVCRCFNWRDGERTMITDETKNAFLVMELVNSDRAEDLENALDFISQQAETFLGV
                     VPEKYLLDCDRPGIAL"
     gene            19175..19654
                     /locus_tag="ACHU66_000407"
     CDS             19175..19654
                     /locus_tag="ACHU66_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR3388720.1"
                     /translation="MWFFLSADRVFLVFHLKVKNSYYKERRPMFTLEAINHGHEQFTG
                     PDFPKLIAYFKDLGMVENIVDIQSGQVTYRSQTGQTLEKTGYQVTIPVSDQANLDQFV
                     TILRNHQAGQTDFPTFCQETAEAGVYKWVIDLEAMTCSYLDQAEQAVFVEAVPSVEN"
     gene            19860..20777
                     /gene="glyQ"
                     /locus_tag="ACHU66_000408"
     CDS             19860..20777
                     /gene="glyQ"
                     /locus_tag="ACHU66_000408"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR3388721.1"
                     /translation="MSKKLTFQEIILTLQQFWNEQGCLLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLER
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEVSDQDMLLENFT
                     KFEKEAERALEEGLVHPAFDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEATRAELLKEDAE"
     gene            20777..22813
                     /gene="glyS"
                     /locus_tag="ACHU66_000409"
     CDS             20777..22813
                     /gene="glyS"
                     /locus_tag="ACHU66_000409"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR3388722.1"
                     /translation="MTKNLLVELGLEEIPAYIVTPSMHQLRDRMATFLTDNRLAFDSI
                     DVFSTPRRLAVRVRGLADQQTDLTEDFKGPAKKIALDADGNFTKAAEGFVRGKGLTTA
                     DIEFREIKGEEYVYVTKHEAGQPAKAVLAGIPEVLKAMTFPVSMNWASNKFAYIRPVH
                     TLTVLLDDEALDMDFLDITSGRISRGHRFLGNETEIASADSYEADLRAQFVITDPAER
                     QNMIVEQIKAIEDKHNVTVEIDEDLLNEVLNLVEYPTAFMGSFDTKYLEVPEEVLVTS
                     MKNHQRYFVVRDKAGKLLPNFISVRNGNDQYLDNVIKGNEKVLVARLEDGEFFWREDQ
                     KLKIADLVERLKVVTFHEKIGSLYEHMERTKVIAEKLADLAGLSAGEKADVARAADIY
                     KFDLLTGMVGEFDELQGIMGEKYALLAGEKPAVAAAIREHYLPNSAEGELPESKVGAV
                     LALADKFDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILEAFGWEIPLDQLIAELYS
                     LNFASLTYDNQPAVMDFIRARVEKMMDKTIPKDIREAVLASSTFVVRLQLAASSAIFQ
                     KAKEADYKEAVENLSRVFNLAEKAEVTAIDEALFENDQEKALAAAVAGLELTEDMAGN
                     LDKLFALSPVIAAFFDNTMVMVDDAAVKANRLTLLKALADKASAVAVFNLLNSK"
     gene            22823..23080
                     /locus_tag="ACHU66_000410"
     CDS             22823..23080
                     /locus_tag="ACHU66_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFR3388723.1"
                     /translation="MEQAKIDRINELARKKKAEGLTEEELQEQAALREEYIEGYRRSV
                     RAHIEGIKVVDEDGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            23219..23590
                     /locus_tag="ACHU66_000411"
     CDS             23219..23590
                     /locus_tag="ACHU66_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388724.1"
                     /translation="MKKLLYFLLFLTYIACAYSTIFFLPFMYFSGIVGATGVGLGLSY
                     SLHQEVKKHPEKQLSEYLPWGSKRDKIMVVTILLFSILVSFIPDVNWHRGLAILLDIS
                     YVLLWQAMLIQFLRKYYFKNA"
     gene            complement(23612..24949)
                     /locus_tag="ACHU66_000412"
     CDS             complement(23612..24949)
                     /locus_tag="ACHU66_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFR3388725.1"
                     /translation="MNDLTKGKPIAVILQFAIPLLIGSFFQLAYNFADSMIVGHTLGK
                     DAFASVGSTASLIFLIIGFAQGVTNGLTIISAQRFGAGDLEGLKKSFVHGLFYASLIS
                     LLLTVSALAFLKPILVLMQTPVSIIDHSHAFLTAMFGGLTFTIFYNFLSSALRSLGNS
                     KTPLLALVIACFINIGLDFFFILVMNWGVFGAGFATILAQACSVLFLIFYIIHKVPHY
                     HIGLADLKLDRGNLKKHAQLAFPMGFQASIIAIGAMTLQFMVNQLGTDAIAAQAIALR
                     TDQLAMLPMVNLGLAIATFTAQNYGAKLYDRIREGVRHSLLLSIAWAIVFAVILILGN
                     RFFSGLFLPNASQTVLDLALVYYIINGSCYWIVASLFILRSFIQGLGKGFIPTLAGFG
                     ELIFRAAVAIIGMQYFGFYGTAAANPAAWIGSIIVLIPSSIIFMKKLKAGQSI"
     gene            complement(25269..25934)
                     /locus_tag="ACHU66_000413"
     CDS             complement(25269..25934)
                     /locus_tag="ACHU66_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFR3388726.1"
                     /translation="MPNYTIGILGLGVFGTTIAKTLHNYDCNIIAIDNHEQRINHLEP
                     ILTRGIVGDITDRSLLRAAGIGNCDAVVVATGENLESSVLAVMHSKVLGVPMVIVKVK
                     GTTAKEVLLRVGADKVISPERETGISLAKQLLHRDTTSLFELEGNVSIVEFHPPVKWI
                     GKTLGELKLRQHYKLNIIGYRSGENQELNIQLTPDYVFKSDELILAVTDHNTVDHFEE
                     LTN"
     gene            complement(25946..27295)
                     /locus_tag="ACHU66_000414"
     CDS             complement(25946..27295)
                     /locus_tag="ACHU66_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFR3388727.1"
                     /translation="MSPFRIKLSPSQRIIISFLFVILTGSILLSLPISQISTSTASYW
                     DHLFTSVSMVCVTGLFTKAVAETYSTFGQIICMMLIQIGGLSLIGFIGLFALRGGRKM
                     NFINMATLQEALNRSDTKYFRNFLKSAFGFTLAVEAFGALLLSFHFVPEFGWARGLFT
                     SIFVAISAFCNAGFDNFGATSIMRYVDNPLINITLASLIIMGGLGFSVWFDLQTQIGQ
                     KRSLRKLGFHTKIVLGLTAIILTLGTLTTLLTEWNNPSTIGQLSFGHKLLASFFQTVT
                     MRTAGFASIDYTKAEPITLLLYIFQMMLGGAPGGTAGGIKITAFLTLVLYARSEILGL
                     PHTNFMGRTIDNLSIRKAFATFSVFLLMFIIGLFALALTDDRQPLLFLMFEVMSALAT
                     VGVTANLTPSLTMAGQAVIMALMFFGRIGPLSILASLSVRKLSKTESLQYAKSSMLV"
     gene            complement(27559..28098)
                     /locus_tag="ACHU66_000415"
     CDS             complement(27559..28098)
                     /locus_tag="ACHU66_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFR3388728.1"
                     /translation="MNSKRQTLNFITMLAAQAVVISLIERLITPPFAFAPGAKLGLGN
                     LISLIAIFTLPTKDSLKVVALRLLISTFLGGTFSTFLYGFAGTTLSYVGMLSAKQLGP
                     NRVSPIGISILGGMLHNLGQLLVFATIARSFYVLNYLPILAFTGILSGLLVGLAATYL
                     LQKVGPLRHYHQLVLAEWK"
     gene            28281..30173
                     /locus_tag="ACHU66_000416"
     CDS             28281..30173
                     /locus_tag="ACHU66_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR3388729.1"
                     /translation="MTKNIVIVGAGYAGIAAARLLGKTFKKDQDVTVTLIDKNSFHTY
                     MTELHEVAAGRVEANAIKYDLQRIFKKYPKVQLVTDKVVEIDYDKKQVVAEHQTLDFD
                     YLLLAMGGEANDFGVKGVKEHGFTLWSIEAAERLHDHMIDACYRAMREHDEAKRRALL
                     TFTVIGAGFTGIEMIGELIDWVPILAREFKLDPKEFSLKVVEATPNILAMVTEKEQVK
                     ARKYLEKKGVELVLGDGVASVQEDSLTLSSGRQIPTYTSIWTAGVQANTDASEFGIEK
                     ARAGRLVANEFMEAKGKENVYVAGDLVYFEESEGKPTPQIVQAAEQTGHTAARNIISA
                     IKGGEKHSYKGKYDGFMVSIGARYGVAFLMDKYHMSGFMAMAVKHMVNLLYFFTIRSF
                     FYMGSYVRHEFFDIQNKRNIFGGHTSGKGNLLWSVPMRVLYGSVWLYEGIKKAFGLFG
                     TTSWFGDQVVFPFPWLADPVSGASAAEAVSSASQAATAAAEAAEPIFGLSYAYGEAPM
                     AVLDKMPDWFATIMEFMMPNQEVALFMQKFMTVAEIGIGLALIAGAFVWIVSAATVAL
                     VVMFSLSGMFYWVNIWFIPAAISLMNGAGRAFGLDYWIMPWLGRFLDKKIYGKPKHIY
                     RIKYKK"
     gene            30192..31181
                     /locus_tag="ACHU66_000417"
     CDS             30192..31181
                     /locus_tag="ACHU66_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFR3388730.1"
                     /translation="MVHQIWNTYPEIERGLEEVKSIILSEMMVLHPAVTSKIVEYVEA
                     PGKYLRAGLCLLFAQMTEGKISRSKLYFAAHLEVLHLATLIHDDVIDGADKRRGVTAA
                     HQQFSNRIAIYTGDYLLAYSSRLFGKGLRLLEVSRDDLDLGNDKLMETILRGELSQLL
                     NQFDANMTMKAYLKQIQGKTAFLFGLACQLGSFVPGQSRKDSSMAFRSGQALGMAFQL
                     RDDLIDYQLDVAESGKPRLQDIQNGIYTAPLLFAMREDKSIQQQLRAYIQSPTAEGLE
                     ILTDRIFATHAIAKTEKLIVSYLEKMMNFVDRIPQLSTASMQNLVAIVFSHYF"
     gene            31260..31739
                     /locus_tag="ACHU66_000418"
     CDS             31260..31739
                     /locus_tag="ACHU66_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HFR3388731.1"
                     /translation="MKTTKVVLKSLVVLGAVFALAACGSKQESASTSSSTKETVVLKD
                     GTYKAESSKDDYGYKIVHTLTVKDGKISESKFDYEAEDGSLKSANEEYNKKMKDKAGV
                     SAGEAIEQLNAALVEKQELSAVEVVSGATHTSEDFKKSTEALLAAAAEGKTETVKLD"
     gene            31817..32866
                     /locus_tag="ACHU66_000419"
     CDS             31817..32866
                     /locus_tag="ACHU66_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFR3388732.1"
                     /translation="MKKIASLLFVGLLMVLLVACGGKQNESLSVVKTPLTRSESLLHT
                     VVQLSIYHENQEEAMDEAVSYIKEMESLLSTNLEGADVYRINQAAGKEAVKVDERTFE
                     VIETAIDMSKESQGLFDISIGAVSNLWKIGDEDARKPSDEEIKAALPFINYKDITLDK
                     EKKTVFIKEGMTLELGAISKGYIADKVRELFASKGITTAIINLGGNVVVMGDSPTTQN
                     GWNVGVQDPDQVRGSTVGSVPGTHDSVVTSGIYERYLEVDGVKYHHILDPKTGYPVEN
                     DISGVTVFSKTSIQGDALSTTLFLLGVEKGLDFINQIDGVEAVFIDKEQGVHLSDGLK
                     ESFELTNEEYHLVNE"
     gene            32859..33815
                     /gene="menA"
                     /locus_tag="ACHU66_000420"
     CDS             32859..33815
                     /gene="menA"
                     /locus_tag="ACHU66_000420"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR3388733.1"
                     /translation="MNKSTKGLSLPVFLEFVEMKTKVASVFPMTLGILWSLYRYQAFN
                     WLNTLLFIIAVLSFDMCTTAINNSMDYHKAKDEAYRQESNVIGKFSLDFRQMIGIVLA
                     LLIFSLLVSLLLVWRTSWLLLPMGALCFLIGIFYTFGPIPLSRMPLGEVFSGVTMGFG
                     IFFLAVFIQQPDLLLTSQVSGQWMTLQFSWIKIIEIIFMSIPLVTLIANIMLANNTCD
                     LEEDIRNHRYTLVYYIGKKNALKLYFVLASLPWLLWIIYCLIGFLPIWALAGLIGVWP
                     AYKSLETFLKKQVKRETFIEAVKSFVLFALLYVVILALALIF"
     gene            complement(34123..34551)
                     /locus_tag="ACHU66_000421"
     CDS             complement(34123..34551)
                     /locus_tag="ACHU66_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFR3388734.1"
                     /translation="MTFKSILKQLKLFDYILIGFTLVLSFLPAIFTYTHLTTDTNEAK
                     TIAYVRINGEVVDQFELSKDTPHQEKTYYPNEGQYNIIEVDGERIRVKEDNSPDQIAV
                     MTSWISQPGQLSVCLPHNLLIEIKSVGGESTDEEELILPL"
     gene            complement(34604..34951)
                     /locus_tag="ACHU66_000422"
     CDS             complement(34604..34951)
                     /locus_tag="ACHU66_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388735.1"
                     /translation="MNVDKPKFILTLIVLSYTAICSLYIHSYLTGDNPPIFILLFTML
                     PILNSDWQTSGPEANAFQKAIYLYHKPLLWFLFAGFALTFSIVFFAGKIQPLHAGFWG
                     MLAILLFFRQLKK"
     gene            complement(34941..35285)
                     /locus_tag="ACHU66_000423"
     CDS             complement(34941..35285)
                     /locus_tag="ACHU66_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR3388736.1"
                     /translation="MNKPFTPKQTCRFFVLFYALFSAFFIYSYMVNEPINGSFLLMLP
                     TIQIIKSYQTAEEKRYFIIQAIFALFAMTLTYSVGIWLGHVKPLILILPAGFAAAILY
                     RIYFKKEKPDER"
     gene            complement(35298..35591)
                     /locus_tag="ACHU66_000424"
                     /pseudo
     CDS             complement(35298..35591)
                     /locus_tag="ACHU66_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            35638..>35928
                     /locus_tag="ACHU66_000425"
     CDS             35638..>35928
                     /locus_tag="ACHU66_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HFR3388737.1"
                     /translation="MAYSLDFRKKVLAYCEKTGSITEASAIFQVSRNTIYQWLKLKEK
                     TGELHHQVKGTKPRKVDRDKLKNYLETHPDAYLTEIASEFDCHPTAIHYALKA"
BASE COUNT        10375 a         6833 c         8267 g        10453 t
ORIGIN      
        1 aaatgagatt tcgagtaaaa aatgaaacaa catgaaagga ttacaaaaac atgtcaaaaa
       61 ctattcaggc cattactaat cagatttggt ccatggccaa tgaattgcga ggaaatatgg
      121 atgcttcgga atataagaac tatatcctag ccttcatgtt ctatcgctat ctatctgagc
      181 atcaagagcg tttcttggtg gaagatgaaa ttatcagtcc tgctccagga gaaactgttc
      241 aggatgccta tgcgcgagaa gcagttggga atgacttggt agagcatttg gaaaatacag
      301 cttctcacct tgggtatgcc atcgagcctg aagatacctg ggcaagactg gtagccaaaa
      361 ttgataacag tgagattgta gcgagtgact accagacgat tttcgatcat tttaacgcca
      421 atgtagaact caatcgggat gctatggaag atttcagagg cgtctttaat gacatcaatc
      481 taggggactc tcgtttgggc aattcaacgg ttgcgcgtgc aaaatccttg aacagtattg
      541 tgaagttgat tgatagcatt gagtataaaa atgacgaagg aaaggatatt ctgggagaaa
      601 tctatgagta tcttattgga cagtttgccg cttcggcagg taaaaaaggt ggagaatttt
      661 ataccccgca tcaagtgagt aagattttag ccaagattgt tactttaggt ttggaaaaat
      721 cagatactag cttctctgtt tacgatccaa ccatgggttc agggtctttg ctactaacag
      781 tgcgtaatga actacctcag ggccagcata tcaaattcta tggccaagaa atgaacacaa
      841 ctacttataa cttggcgcgg atgaacctga tgatgcatca ggtcagctac agcaatatga
      901 ttttgaataa tgctgatacc ttagaaagtg actggccgga cggggtcgat gaacttggaa
      961 tcgatcagcc acgtagtttt gatgcggtcg tcgcaaaccc accctactct gctaaatggg
     1021 acaatcgtga gtccaaattg aaggatccac ggtttatgga atacggtaag ctagcaccag
     1081 cctctaaagc ggactttgct tttatcttac acagtcttta tcatctgaat aatacaggga
     1141 ccatggccat tgttttacca cacggagtgc tctttagggg agcagcagaa ggtcatattc
     1201 gcaaactgat tattgagaaa aattacctgg atgcagttat tggcttgcca gctaacctgt
     1261 tttatggcac aggcattcca accactattc tggtcttcaa aaaaaatcgt cagaccaagg
     1321 atgtcttctt tatcgatgcg agcaaggagt ttgaaaaagg gaagaaccag aatcaccttt
     1381 ccgatgatat ggtagaaaaa atagtagaga cctatcacaa tcgccagtct gttgacaaat
     1441 atgctcattt ggcatcaata gaagaaattg tagaaaacga ctacaatcta aatattcctc
     1501 gttacgtcga tacttttgag gaagaggaag agattgatct cgggcaagta acgcagcaac
     1561 tagaacaaga tagactagag attcgggctc tagaagataa gattcgtcaa caattaaaga
     1621 ctttgggagt agatttttag ataaatgaaa agagcgagta ggatttttcc tcgctctttc
     1681 tttgtatgtc ttaaggttta atcacttcta ccccacccat gtatggacgg agaacttctg
     1741 gaatggtgac agaaccgtct tcattttgat agttttcaag gattgccgca acggtacgac
     1801 caactgctag accagaaccg ttgagggtgt ggagcaattt gaccttgccg tcagcagcat
     1861 cgcggtagcg gatttgggca cgacgagctt ggaaatcctc ggtatttgaa cagcttgaga
     1921 tttcacggta ggcattttgg gcaggaatcc agacttccaa gtcgtaggtc ttagcagctg
     1981 agaagcccat atcacctgta cagagggcca aaacacggta aggcaagcct agtttttgaa
     2041 ggatattttc tgcgtttgcg gtcatttttt ccaattcatc ataagactgt tctggtgtgg
     2101 caaatttgac catttcaacc ttgtggaatt ggtgaaggcg gataagacca cgggtgtcac
     2161 gaccagcaga accagcttca gaacggaaag atgggctcat ggctgtgaag tagattggca
     2221 ggtctttttc ttccagaatc tcgttgcggt agtagttagt caacggcact tccgcagttg
     2281 ggatgaggac gaagttagtg tctgccaact caaaagtatc ttccttgaac ttaggatatt
     2341 gaccagtacc gaacatagag tcgtggttga ccatgtaagg tgtgatgatt tcttggtagc
     2401 cttctgcgat atgctcgtcc aacatgaagt tgtagagggc acgttctaag cgagcaccca
     2461 agttcttgta gaaaaggaag cgtgcaccag ttacttttgc gccacgttcc cagtccaaaa
     2521 tatccaggtc ttcgcccaaa tcccagtgag cttttggctc gaaagtgaat tcacgaggtg
     2581 ttccccaacg gcgaacctct acgttttctt cttcatccgc accaactgga acagaatcgt
     2641 gcggcgtatt tggaagaacg gtgatgatgg cgtttaattc ttcatcgatt tccaaaagct
     2701 gagcgtctaa gtttttcaca tccgcagaca gtttctgcat ggcagcaatc ttgtcgtcag
     2761 cgttttcctt attgcgtttt gcttgggcaa tctccgcaga aacggtgttg cgctctgctt
     2821 ttaattcttc tacagtaacc aagatttcac ggcgttttgc atccaattcc ttgatgtttg
     2881 ccaaggtttc agcagccaca ccacgagtag ctaatttggc agccactccg tcaaaatcag
     2941 tacgaatacg tttgatatct agcatagatg tctcctttag aataaaaaag cacagaatga
     3001 gagatgctgg agcgatattt accgcggttc catccagctt cacattctgt gcacttgatt
     3061 gtattttgat aatttcataa cggtagaatt tcacaacttt ttcctatctg ctcacagcga
     3121 ccgcagactt tctggaagga gccagatgct acttatccgt ttctactatt atacttgaat
     3181 ttttattttt tggcaaatag attcgaaatt tttctgccga ttgtttgcat aagagtcggt
     3241 aatttcacaa ctttttcatt gccgatatgc tcacgggcaa ttttaagaat cttaccagaa
     3301 tctttagatg caaacaagaa tcgacttttc tctgtataaa tttcaaaatg acggctgatt
     3361 gctttgccag aaacgtttgc gccaatgcta gtcatttctg accaaggaat ctggatatag
     3421 cgttcaacgt tgctatctgg gtagaattca agtgccttgt caccaattag aaatttacca
     3481 accttgccac caaagcccat gtaggaaacc cctgtggttt gtagttcaac gcttgtattt
     3541 tgtgattgtg ccatagtgct ctctccctag atattcatta tatcataaaa ccccgcaaga
     3601 gcggggtcct tgattacata attccgaaga aacgagcaag aatacctacg atgaagagac
     3661 cgataatcat tgtgattggt gttactttct tcttaagcaa gtgcatgcag aggaaagtaa
     3721 ggagaagtcc catcaaacca ggaatcaagc tgtccaactg accttgtaat gtttgagctt
     3781 gagttggttc aaggctaagt ccgctaagag cttgaccaag aatacctttc aactcagcac
     3841 cagtaacagt accttctggg aagacgatgt aagcaccttc tgacaattgt ttagcaggaa
     3901 gatctacagc gaaagtaata gatacccaac gttgaacaag aacggcaagg atgaacatac
     3961 caaggattga agcaccttta gtgatgtctt gaaggatacc accagacatg tctttagtga
     4021 tttcgctacc agctttgtaa ccaaattctt gtgtatacca taggaaggcc atacgaatga
     4081 tgttccagcc aaggaagaag atgattggtc ccatgatatt tccagatagt gcaagtgaag
     4141 caccaagagc accaaggatt ggacgaacag taaaccagaa gactgggtca ccgataccag
     4201 caagaggtcc catcataccg attttcacac cttggatagc tgtgtcatcg atttcagcac
     4261 cattagcacg ctcttcttca agggcaagag ttacaccaag gattggtgaa gcaacgtatg
     4321 ggtgtgtgtt gaagaattcc aagtggcgct caagagcagc agcttgatct tcttttttag
     4381 tgtaaagttt tttgatagct gggatcattg cataagccca acctaagttt tgcatacgct
     4441 catagttcca tgaaccttga aggaaggttg aacgccacca aactttttta cgatcgctta
     4501 cggaaagttg aattttttct gacatgatga atctcccttc tcttagtagt cttcaaggat
     4561 gtcgccgatt gggtcgttag atgaagcacc accgttacct gatccgcctt gttttgacaa
     4621 gttgaggtag ataagagcga ttgcaacacc gataacacca agtgcgataa gtgtcaattt
     4681 gctgatagca gcaaatgcaa aaccaagtgc gaagaatggc catacttcac gagttgccat
     4741 catgttgata accatagcat aacctacggc aacaaccata ccaccaccaa cggccatacc
     4801 acctgacaac caagctggca tagattcaag aactgcttga acagcttctg caggaattgc
     4861 aagaagaaga gccgcaggaa ctgcgatacg caaaccttga aggagaagag caaacaagtg
     4921 gtaacgttct acagcagcga agtttgcatc tttagcagca ttgtcagcac cgtgtacaag
     4981 accgactgag atagtacgaa cgatcattgt aaggaaaaga ccagctacgg caagagggat
     5041 agcagttgtt gttgcaacgg caataccttc agttgagaag tcgccacctt tgatcatgat
     5101 gattgcagca gctacagaag caagagcagc gtcaggtgca acagctgcac caatgtttgc
     5161 ccaaccaagt gcaatcattt gtagagaacc accaagcata acacctgcag ccaagttacc
     5221 agtcacaaga ccgataaggg tacaagctac caatggttga tggaattgga attcgtcaag
     5281 gataccttca agaccagcga agaaggcaac gatgacaacg agaattgcag aaataagtgt
     5341 aatatctgac atgttgtaat cctttctata ctaacagatc gatttattga acgtttgcct
     5401 tgttgataag ttcaaacaag tctttcttcg aatcgtttgg tactttacgc acatcaaatt
     5461 caacgccaag gtcacgcaat ttttcaaagg ttgcaacgtc atctttgtcc attgacaata
     5521 cgttgttaac cattgtttta ccagttgagt gagccataga accaacgttc aattctttga
     5581 tttcaacacc accttcaatt gcttcaagag cttcttgagg agtttcgaaa agaatcaagg
     5641 cgtgagtgtt accgaatcga gggtctttag aaacctcaat caatttcttg attgggacaa
     5701 cgttcgcttt tacgttacca ggtgcagctt gtttgatgag ttgtttacgc aattcatctt
     5761 tggcaactgt atctgataca acgatgatac gatctgcttt ggaagcagga gtccaaccag
     5821 tcgccacttg accgtgcaag agacgagtgt cgatacgggc taagttgatt ttcaacttgc
     5881 catcaccgat aacagtacct ggagggatag caccttgtag tgcaacagga gcagcatcgg
     5941 caacaggagt agcttcagct ggttgaagtt cttctggaag aactttgata ccatctttgg
     6001 cttccttgat aatgtttgcc gcaacttctt ccacgccagc agcagcgtta atcatacgct
     6061 ctgtgtaagc ttggataagc attggcaagt ttagaccggt gatgatagca aacttacgat
     6121 ctggattttc accagctaca cggcttgctt ggttgaatgg tgaaccactc caaaggtcag
     6181 ccaaaaccaa aacttcatca tcggcatcga atgcgtccac ggcagcattt agcttagcgt
     6241 ataaatcatc tggaccttca cttggcataa aagtaacaac ttgtactttt tcttgttcgc
     6301 caaagatcat tgaacctgac tgatgaatac cagcagcaaa ttcgccatga cttgcaataa
     6361 tgattccaat actcattatt gatcctccta aatttttctt gttcatgtaa aaaccattga
     6421 atgggctttc atgttcgaac tataccatta taaaacgttt tcagtacatt tgtcaagtag
     6481 gtttgtgtta aaagtgcaaa atatttccaa aacttttcat aatcatgttt cagaaaagtg
     6541 ataaaaatat gcaacgaata tgacaaaaac ctgacagttt atcaggtttt ttgatgtcta
     6601 taaaaatagg ttttctagtt cacgtgcgac accatcttca aaattagcaa atgctgtcaa
     6661 tctgtcggca tgtggacgaa gtgtatcgct acaatttttc atggcatagg ctgttcctgc
     6721 taggtctagc atctcaacat cgttgtgctc atcgccgaag gcaattaagt cgctaggagt
     6781 ggcttggtag aggttaagaa catagctcag agcggttgcc ttattgacac cttttgcaca
     6841 agtttcgagg atatttaatg gtccgcccca cgtattgatt tctaactcat ctttgaaata
     6901 ggcattcatt tcttttgcaa gttcatactt atcagttgca cgagtctgca taaggatgga
     6961 atgaggatca ctagtgatta attcaggttt gatgagtgta tctggagtaa tctgttcaac
     7021 ccccatcaga gccggatcaa tcttatctaa gttcttttga gtgatgtaga atttattctt
     7081 gtattcacca gcaataaaat ctgcctcgaa tgtttcttct tcttttagaa attcgagtag
     7141 gtattttttg tcaattaaaa tattttgttc ccattgccat ttctttccag ggatgtgaac
     7201 taaagaaccg ttgaaattaa tcatgggagt atccagttct agttgttggt aaaatttttc
     7261 agccattcga taggggcgac ctgtggcgat aagaacggtg tgtcctgctt ggcggacctt
     7321 ctttatagtt gagatggtat aatcagataa ttggctatca ttattgagca atgtgccgtc
     7381 caaatcgata gctatgattt ttttcttcat atttaatagc attcctttct agagttgtta
     7441 tactagtttt taatagtaaa taatgtttag ctagtagcta gattgctgag aaattgtttt
     7501 atgcgtcaaa gaatagtact tgtaggtaac catatcttga actaataact tccagtataa
     7561 caaaaggaga taataaaagc aaagaagcga taaattttgt atagattttt gtaagtctgc
     7621 aacgttcggg atttgcgaat gtttaaagtt gaaaatcaat aaaataacgc tttcaagatt
     7681 aaaaaacttg aaattataat gttttttgat tataatgttt ttattgttcg taaattaaaa
     7741 ggagaaatga aaaaaatgga tcaatttttt aaattgaaag aacatggaac gactgtttct
     7801 acggaaatta tggcaggcct tacgactttc tttgccatgt catatatctt gtttgttaac
     7861 cctagtgtgt taagtgttgc tggaatgcca gcgcaggctg ttttcttagc gactattatt
     7921 gccagtgcag tttcaacgct tgttatggga ttatttgcta acgtacctta cgccttggca
     7981 caggtatggg gttgaatgcc ttctttactt atacagtggt tattggtctt ggttttactt
     8041 ggcaagaagc tttggcaatg gttttcctct gcggtttgtt taacgttttt atcacagtta
     8101 caaaggttcg taagagtatt atcaaggcga ttccagttag tttacaacat gccatcggtg
     8161 gaggtatcgg ggttttcgtt gcctatcttg gttttaaaaa ctctaattta atcaccttct
     8221 tgacatctgg ttcagatatt attactgtaa atggtgttgc tccggcagat gcaacagccg
     8281 aaacattctc aaacggtgtt ttctcagttt ttgctggtgg tggtgtcgta cctggtattt
     8341 caacctttac tgatccaagt gttctcttga cagtgttcgg tcttcttttg acagctgttc
     8401 tggttattaa gaacgtgcgt ggtgcaattt tgattggtat tattgcaacg actttagctg
     8461 gaattccagc aggtgttgtg gacctgtcta ccatcgattt tgcaaataac aatatcggta
     8521 cagcctttgc ggagcttggt acaactttct tagcggcttt tgatggtcta tcttcgctct
     8581 ttgctgactc aagccgtcta ccacttgttt tgatgactat ctttgcattc agcttgtcag
     8641 atacctttga tacacttggt acctttatcg gtacaggtcg taagactggt attttctcag
     8701 aagaagatga gaaagctctc gaaaatggta caggctttaa ctctaagatg gacaaggcgc
     8761 tctttgcaga tgcgattggt acctctatcg gtgcactatt tggtacttca aatacaacga
     8821 catatgttga atcggctgca ggtatctctg caggtggtcg tactggtctg acagctgtta
     8881 caactgccgt tctcttcttg ctatcaattt tgattttacc attcattgga attgtgccaa
     8941 atgctgcaac agctccagcc ttgattatcg tcggtgtcat gatggtttca tcctttcttg
     9001 atgttgattg gagccagttt gaagacgctc ttccagcctt ctttgccgcc ttcttcatgg
     9061 cgctttgctt ctctatctca tacggtattg cagccgcatt tatcttctat tgcttagtaa
     9121 aagtttcaac tggtaaggct aaagaaattc atccaatcct ttggggtgcc acagctctat
     9181 ttattctgaa tttcatcatt ctagccatct tgtaatctgt ttattatcct tgaactgatc
     9241 ttcatttgaa ggtcagtttt tttatttatg attaaaatat atgggaatca atgtgattta
     9301 ttcagcatat gcggtattga acgttgtaat ttgaaaacgg atggagttta tttttccatt
     9361 tcgttgcttc tctgtctcct cttagtgcca tttttactcc ttgtgactgt attttaaatg
     9421 gctgttcgtt attgtaagca agatgtctca tacctagaca tccgctggat taatgtcatg
     9481 atggggtgaa aaaattatca tgtagtcttt gggagtcaca gggtatagaa taacccctga
     9541 gcttgggttc agggggagtt ttctaactat aatgtttgaa taacgaatga gttgcttgat
     9601 ggatggtgcg ggccgtttcg gcattgttca tagtgtagag atggatacca gccacatcat
     9661 tggtcactaa gtcaacaatt tgatctacag cataggccag acctgctgca cgaagtgact
     9721 caggattgtg ctcatatttt tccaggatag ctttaaattt acgtggtaag cgaatgttct
     9781 cgcaggtttt caagagacga agtgcctgat tcctgttgat gataggcata atacctgcga
     9841 taataggcac ttcgatgtct gctagagaac atttctcctg aaagttataa aatgcttcgt
     9901 tatcgaagaa gagttgagtt accaaggttg agcaaccggc atcaactttc tttttcaaat
     9961 ttttgatgtc tgagacagaa tttggtgatt ctggatgtac ttctggataa caggctccaa
    10021 taatgtcaaa atgtggggct tcttccttga taaaggaaat caagtcagtt gcatactgga
    10081 agtcctcttt agggggaaga ccttcaataa tatcgccacg aagtgctaaa atctgatgta
    10141 caccaatttc atcgagagaa tgaagaacgg atcggacctt ttcttttgtc aagtaagcag
    10201 ctggcaaatg tgcgatagtt ggaatatgca attcattgcg aacatggtta gccaacttga
    10261 cagtagtctc ttctacgttc aaattgttat tgctgcatgt aacactgata aaatgagggg
    10321 ccaaaccttg catctcatcg agagtttgga taatcttttc attgcctact tctggttttg
    10381 gaggaaaaat ttcaaatgag agacttgggg tttgaccaat cataaattag agttcctttc
    10441 tggctgtctt agctgcttga gtcaagtgga taagactagc ttttgtttct gattcgccac
    10501 gagtcttgag tccgcagtct gggttaatcc aaactttttc tttcggtact ttagcaagga
    10561 tggcttgaat ggtgtgtgca atttcatcaa cggcagggac acgtggagag tggatgtcat
    10621 agacaccagg acctacctga gtttggaagt ttttagcctt gagttcatct agaataacaa
    10681 gatttgaacg gctggcttca aaggagataa cgtctgcatc catattgtcg atggcaggaa
    10741 tgatgtcagt aaattcgcta tagcacatgt gagtgtgaat ttgtgtctct ggtgcaacag
    10801 ttgagtgaac taatcggaag gctggaatcg cccaatccaa atattcactg taccagtcgc
    10861 tacgacgaag tggtaatttt tcacgtaaag ctgcttcgtc aatttggata atcttgatac
    10921 ccgctgcttc aaggtcaaga acttcttcct tgatagcaag ggcgatttga agagttgatt
    10981 ctttaataga aatatcttca cgagggaatg accagttgag aatggttact ggccctgtca
    11041 acataccttt aactggtttg tttgtacggc tttgtgcata gcttgaccat ttaacagtga
    11101 ttgggttcaa gcgcgtcaca tccccccaga tgattggtgg ttttacccca cgcataccat
    11161 atgattggac ccatccgttc ttactgaaga gataaccaga aaggttttca ccgaagtatt
    11221 ctaccatgtc gtttcgttca aactcaccat gaaccaagac atcgaagtca acttcttcct
    11281 gccatgcaat ccattcgtct gtttgagctt taacaaatgt atcgtattct tcttctgaga
    11341 tatttccttt gcggaaagcc aagcgtgtgc tacggacttc cttggtctga gggaaggaac
    11401 cgatagtggt tgttggaaga agtggtaagt ttagcttctc gcgttgaatg gcttcacgct
    11461 ctgcaaagac tggtaggcgt gtataatcgg cgtctgtcag tcctgcaaga cggtctgcaa
    11521 gggctgcatc acgaccaaca cgctcaaggg caaagagttc cttgttagct gtgagagcaa
    11581 cttcgccttg actgttacgg atggcatcca aatcacgcaa ttcgctcagt ttttcaactg
    11641 caaaagcaaa gtggttgagg atagctggtt caaattcttc atttgctgtt gtgaaaggta
    11701 catgaagaag ggagcaagaa gttgttataa cgacatgttc agctggaata gcatccaaaa
    11761 cagccaggct cttttcgtag ttgttgcgcc agatattttt accgttgacg ataccagcgt
    11821 agagggtctt gttagctggg aaaccagttg caacaagtgc agcagtttcc ttaccttcta
    11881 caaagtcaag accgatggca tcaactggta ggctggtcaa ttctgtgtaa atatcacgaa
    11941 catcaccgaa gtaggtttgg atgagaactt gcaaaccttt cttatctgcc aagagtgttt
    12001 ggtagatacg taggaaaagg gctttttctt cagcagtcaa atccttgacc aagctcggtt
    12061 cgtccagctg gattttttca gcaccaagct ctgccaattt ctcaaagact tgtccgtagg
    12121 ctgcaactaa gctatcagcg aaatcagtag cagttaaacc ttcttcaaaa tcagttaatt
    12181 gtaagagtgt gaatggacca atcaaaactg gacgagttgt gatgcccaag ttctttgctt
    12241 cttggaattc atcgaaaatc ttatgaccgg ctagtttgat ttctgtatct ttttcaatag
    12301 ctggaacgat atagtggtag ttggtgtgga accatttttt catcggtcta gcacgaacgt
    12361 cccctttttc tccttgataa ccacgtgcaa gagcgaagta tttttcaagg tcagtcaagt
    12421 caaggttctg tactgcttta ggtacgatgt tgaagagaac agctgcatcc aaaacattgt
    12481 catagtggga gaagtcgttg cttgggattt cagtaatgcc tgcttcttta acaatattcc
    12541 agtgcttagc acgtaggtct ttagctactt ccaaaagctc ttcttgtgga atttcatttc
    12601 taaagtattt ttcagtgata aattttaact cacggtgttc tccgatacgt gggaagccga
    12661 taatagtagt agtcatgttt tttcctccaa aatatttcta tgcccctatc ctaacagaaa
    12721 aaacgactct tgtataattg taaaaaatta gagggagtta taacttttga ctataacaga
    12781 ttgaaagaga gctacttttt tgatatttct tctatataaa agcttgaaaa aagcctgttt
    12841 atcaagcttg acttttttat gctggctcca gtatagtttt ttcttaagcg tggtatagat
    12901 tttatctatg aaactgctaa aggcaaactc ttgtttatga gcaattgttt ataggtagtt
    12961 ctgatagtta ttttttatca agccataaaa cttctatatt agacagggct aggtagccat
    13021 gctacaattg aaaaaatgaa agaaaggggc tttttatggg aaaagttatc attggaattt
    13081 caggaaacga gcaagaattt ccaactaaat ctggtagaac ttatgtaacc gttgcgagtg
    13141 aattggcaga tggtgtgcgt caggcaggtg gtgtaccaat ggttattcct atgggaactc
    13201 ctgacttagc caaggactat atcaacatga ttgataagtt gattttatca ggcggtcagc
    13261 atgtagaccc cagtctatac gggcaagagc gcttgattga tagtaatgat tacctcttgg
    13321 aacgggatgc atttgagtta gctttgattg aagaggccct tcggcaagga aaacctattt
    13381 ttgccgtttg ccgtggtatg cagttgctca atgttgctct aggtggtagt ttagagcagg
    13441 aagtggctca tcattggcaa gagggtattg cggggacatc ccatcgttta caagtgaaac
    13501 ctaaaagtcg aatcggtcaa ctgtttaccc aagggagccc tattaattcc ttccatcagc
    13561 aacgaatcaa agaattggct ccaggactgg tggcgacagg tttggatcct agagatggta
    13621 cgatagaagc ttatgaaagt agagggaacc aagctttgtt tggtatccaa tggcattcag
    13681 aatttttgta taatgattgt aagcaacatc gagacctgtt tcagtattta gtagatatat
    13741 tttaagaggg gtatccctct ttttattatc aatgcttata attgcaaagg tatttgttat
    13801 aacgaaaagt tataactttc tataaaaaaa gtcaattagt cagaaagaaa attctgtgtt
    13861 aagatagata cagttctaat ttaaggagat cattttatga aattaaaaaa attgtttagt
    13921 ttggcaacag ctgctttatc agtaggtgta cttgttgcat gtggtaactc ttcttcaagt
    13981 tcagattcat ctgcaacaac tgttcgagta ggtgtgatga gcttgagcga ttctgagcaa
    14041 gcccgttggg ataaagttca agaaattctt ggtgatgaag tgaaattgga gttcactcaa
    14101 tttacagatt actcacaacc aaacaaggca gttgctgaaa atgaagtaga catcaacgca
    14161 ttccagcact acaacttctt gaacaactgg aatcaagaaa atggtgaaga cttggttgct
    14221 attgcggata cttatatcgc accaatccgt ctttactcag gtacagctga tggaaaaaac
    14281 aagtacacta aagtggaaga aattccagat ggtgcagaaa ttgcggttcc aaacgacccg
    14341 acaaacgaaa gtcgtgctct ttaccttctt caagcagctg gtttgatcaa ggttggtgta
    14401 tctggtacag aattggcaac tattgctgat attactgaaa acaagaaaaa cttgaaaatt
    14461 actgagttgg atgcaagcca aacagcaagt tcacttagct cagttgatgc agcagttgta
    14521 aacaatacat ttgtgttgga agctggtttg gattacaaaa atgcccttta caaagagcaa
    14581 aaagatgaaa attcaaaaca gtggtacaac ttgattgcag ctcgtagcga ttgggaaaaa
    14641 tcagagcaag cagcagcgat taagaaaatt atcgaagcct accacacgga tgaagtgaaa
    14701 aaagttattg aagagacttc agacggtatg gatgagccag tttggtaaaa tgtaaatagg
    14761 ttgaggactg ggcagaattg ttcagccctc tatctgtttt tatagagata agaaaaaatc
    14821 tcttaaccaa agtcagaaat gtgggaggat atttgatttt ggttagtaga tttaattgat
    14881 tttcattgag tatgaggaga aagtatggca gatagtaaag ttcaagcatt tgaaaatgat
    14941 gcgattattc aaaactattt tgaaaagttg aaggtcttga tttcaaagaa atctattttt
    15001 gctcagcaga ttggtttgtt ggatgtggcg acttatttga aagatatgtt tgaagaggct
    15061 ggggcagatg ttgttttaga cgacagctat gccgcaccat ttgtcatggc aacgtttaag
    15121 gcttcagtgc cagatgcgaa aacattgatt ttctacaatc attatgatac agtacctgca
    15181 gatgccgatc aagtctggga gaagggcaac cccttcgaat tgaccatttc cgatggctat
    15241 atctatggtc gcggggtaga tgatgataag ggccatatca cagctcgtct gtcggccttg
    15301 aaaaaatatc aagctagaca gaatggcgat ttaccagtca atgtgatttt tattatggag
    15361 ggggcagagg agtcagcttc tgtcgacttg gacaaatacc tttcaaaata taaagaatat
    15421 ttgattggtg ctgatttgct tgtctgggag caggggcatc gcaatagcct acatcagcta
    15481 gaaattgctg ggggaaataa gggaattgtg accttcgatc ttcaggtcaa atcagctgac
    15541 ttggatattc attcttccta tggaggtgtc attgattcgg caagctggta tttattatca
    15601 gcacttcaat ccatgcgggc tgcagatggg gaaattctgg tagatggcat ttacgagcaa
    15661 gtgcaagagc caaatgagcg tgaattggcc ttggtggaag aatttgcact ggctaccagt
    15721 cagtctatga ctgatattta tggactaacc cttccaacct tggtagaaga tcgtcgtgag
    15781 tttctgaagc gtttgtattt tgaaccatcg attaccattg aaggcttgtc aactggctat
    15841 cttggtcaag gtgtcaagac catcatacca gctcaggctt ctgctaagat ggaagtccgt
    15901 ctggtacctg ggttggagcc gcatgatgta ttagataaga ttcgacagca tttggacaaa
    15961 catggttttg acaaagttga gcttgtcttc actctaggag agatgagcta ccgaagtgat
    16021 atgtcgcacc ctgccattgt caatgtgatt gaattggcca aaaaattgac accagaagga
    16081 gttgctgtct tgccgacgtc accggggaca ggacccatgc acacagtctt tcatgctcta
    16141 ggtgtgccga ttgcaggatt tggtctagga aatgccaaca gtcgcgacca tgcaggtgat
    16201 gaaaatgtca gcatcgctga ctactatagc catgttgaat tagtagagga gttaattgca
    16261 agttatgagt aaggaaatga ttaagctaga taatattgat gtaactttcc agcagaaaaa
    16321 acgtacaatc gaagcggtaa aggatgtgac catccatatc aatcaagggg atatttacgg
    16381 tattgtaggg tattctggag cagggaaatc tacccttgtt cgagtgatta atttattgca
    16441 ggtgccgaca gcgggtaaaa ttactatcga tgaggatgta atttacgagg gagaaaaggt
    16501 cacacttacc ccaactcaat tgcggagcaa acgccgtgaa ataggcatga ttttccagca
    16561 tttcaatctg atggcgcaaa tgacggcaga agaaaacgta gcctttgccc ttaagcattc
    16621 gggtttgagc aaggaagaga agaaagaaaa agtagccaaa ctcttggact tggttggact
    16681 ttctgaccgt gctgaaaact atccagctca gctgtctggt ggtcaaaagc aacgtgttgc
    16741 cattgcgcgt gccttggcca atgacccaaa aatcttgatt tcagatgagt caacttctgc
    16801 cttggatccg aaaacaacta agcagatttt ggcactcttg caggaattga atgaaaaatt
    16861 gggcctgacc attgttatga ttacccatga gatgcagatt gtaaaagata tttgtaaccg
    16921 tgtggcggtc atgcaggatg gtcgcttgat tgaagaaggt tcagttttag aaattttctc
    16981 tcatccaaaa gaagaattga cacaggattt catcaaaaca gcaacaggaa ttgatgaggc
    17041 cctggtgaaa atttaccagc aggatattgt taagaatttg cctgagaaca gtatcttagt
    17101 tcaattgaaa tatgctggtt caaatacgga tacggcgatt gtcaatgatt tgtataaatt
    17161 ctatcaagta tctgccaata ttctttatgg caatattgaa attttagacc atacgccagt
    17221 cggtgagatg gtggtgatct tatcaggtga acctggtcag ttgcaccgtg cgattgaagc
    17281 agtgacggag gcgcgtgtgg aagtgacgat tttgaaagga gcgaactaga tgttagaatg
    17341 gattcaaacg aattttccag atatttataa attgggctgg gatggtcaga caggttggtt
    17401 aacacatttt aatttgaccc tctatatgac ctttgtttcc tttgcttttg gtggatttat
    17461 gggcttggtg tctggtttgt tcttggtctt gacaggtcca cgtggggtta ttgccaataa
    17521 gactgcctat tggattttgg acaaggtggc ttctatcttc cgtgccattc cttttattat
    17581 cttgttggcg gctattgctc cttttacgaa aattattgtt ggaaaaacca ttggtacaga
    17641 ggcggctttg gtgccgctcg ccctttcagt ctttccgttc tttgcccgtc aggttgaagt
    17701 ggtcttatca gaattggacc gtggtgtcat tgaggcggct caggcttccg gtgctacctt
    17761 ctgggatata gtcctagtct atctacgtga gggtctgcct gacttgattc gtgtgacgac
    17821 ctttgccttg gtttccttgg tcggctatac agccatggca ggagctatcg gagcaggtgg
    17881 tttgggacaa gttgccctat catacggcta cctacggtat aataatgatg tgaccttttt
    17941 agcaactctt ttgattttag ttatcatttt cgtaattcag tttattggag atttcttgac
    18001 gagaaagatt agtcatagat agttatttag ctgtgttttt tcaaattttg gatagtcctt
    18061 gacttatttg aagcattttt atacaataaa tttaattaaa taaacactgc caccgggtag
    18121 tcatgcaaca gcattttatg aacgtcgtta ggtcttagaa gatgttcata gggtgctgat
    18181 ttttctataa aaggagtgag aatatgtcac aatttgttgt tgattcatct ttttggagtc
    18241 tatttccaga tgcaaaaatt ggagttctat tattgaaaga ttataaaacg ccggcgcaat
    18301 cgcctgatga attagtgaaa ttactggaag aaagcaacgg aattgcccag aaatttctta
    18361 cggaagatac ctttagtgag aatgaggtca ttcgtacata tcgtcaagcc tatcaaaaat
    18421 ttaaaacaaa aaaaggcgcg cgttctagta tcgaagcact tttgaaacgt tcaactagtg
    18481 accgtccagt cacaacaatt tctccattag tggacattta taatgcggcg agtttacgtt
    18541 ttggtcttcc atgtggcgct gaagatttag atacatttgt aggcaatctc caactaacgg
    18601 tcactgaggg tggggatgaa ttctatctca ttggtgatga aaccaataat ccaactttac
    18661 caggtgaagt ttgctataag gatgataaag gcgccgtttg tcgctgtttc aattggcgcg
    18721 atggtgagcg aaccatgatt acggatgaga caaagaatgc gttcttggtt atggagttgg
    18781 tcaattccga tagagcggaa gatttggaaa atgcccttga cttcatcagt cagcaagcag
    18841 agaccttctt gggagtagtg ccagaaaaat atctactaga ttgtgatagg cctggaatag
    18901 ccttgtagga tttgtcagtg ggattgtgtt cggatagata gtggtctttc tagacaaatg
    18961 ctgatttttc tgctacaata ctaagcatga aagaaggttg tttcaaaagc ctgtctagcg
    19021 agcttgggag agtgaaagcc aagccagtct tgaaataatt ggcgctttcc cagtcgtgac
    19081 catgtccgac tcaatcagaa tcatacaaat gaagtaataa attagggtgg aaccgcgttc
    19141 tcaacgcccc tatgtctgtt gacaggggaa gagaatgtgg ttctttttgt ctgccgaccg
    19201 agtatttctt gtttttcatc tcaaggtgaa aaacagttac tataaagaaa ggagaccaat
    19261 gttcacctta gaagctatca atcacgggca tgagcagttt actgggcctg attttcccaa
    19321 gctgattgcc tattttaagg acttgggtat ggtggaaaat attgtggata tccagtctgg
    19381 acaggttacc tatcgctcac agacagggca gactctggaa aagacgggct atcaagtaac
    19441 gatacctgtt tcggaccaag ccaatctgga ccagtttgtg actatcttac gcaatcatca
    19501 ggctggacaa acagattttc ccaccttctg tcaagaaaca gcagaagcag gcgtttacaa
    19561 gtgggtcatc gacctagaag ccatgacctg ttcctatctg gaccaggcgg agcaagctgt
    19621 ttttgtggaa gctgtaccga gtgtggagaa ctagcaattt tgaaatataa atagaaagag
    19681 gatgttatgt caaaaaaact tacttttcac atcgtcagtg gcatagtctc ctttcagtcg
    19741 aattgccaaa cgctcatcgc tatggcgact gagctagacg ttgtactagt tcgcctttcc
    19801 tagcggtatc ggctaggaag gctcacgtcg caatcaattt atatagaaag aggaaatata
    19861 tgtctaaaaa attaacattc caggaaatca tcctgacctt acaacaattt tggaatgagc
    19921 agggctgctt gctcatgcag gcttatgata cggaaaaagg tgcggggact atgagtccat
    19981 acactttctt gcgtgctatc ggtcctgagc cttggaatgc ggcttatgtt gagccttcgc
    20041 gtcgtcctgc ggatggtcgt tacggggaaa acccaaaccg tctctatcaa caccatcaat
    20101 tccaagtggt tatgaaacca tctccatcta acatccaaga actctacttg gaatctttgg
    20161 agcgtttggg gattaatcct ttggagcatg atattcgttt cgttgaggac aactgggaaa
    20221 acccgtcaac aggttctgct ggtctgggct gggaagtttg gttggacggt atggaaatca
    20281 cacagtttac ctacttccaa caggttggtg gtttggcgac aggtccagtc acagccgaag
    20341 taacttatgg tttggaacgt ttggcatcct atatccaaga agttgactcc gtttatgata
    20401 ttgagtgggc tgacggtgtc aaatacggcg agatctttat ccagccagaa tatgagcatt
    20461 caaaatactc ctttgaagta tcggaccaag atatgctcct tgaaaatttc actaagtttg
    20521 aaaaagaagc agagcgggct ttggaagagg gcttggttca cccagccttt gactatgttc
    20581 tcaagtgctc tcacaccttt aacctgctcg atgcccgtgg agccgtatct gtaaccgagc
    20641 gtgcaggcta cattgcccgt atccgtaacc ttgcccgtgt cgttgccaag acctttgtgg
    20701 cagagcggaa aaaactcggt ttcccacttt tggatgaagc aacacgagca gaattgttga
    20761 aggaggacgc agaataatga caaagaattt acttgttgaa cttggtttgg aagaaattcc
    20821 tgcctacatc gtaaccccat ccatgcacca gttgcgtgac cgcatggcga cttttttgac
    20881 agacaatcgc ttggcttttg atagcattga tgtcttttca acgccacgcc gtttggctgt
    20941 tcgtgtccgt ggtttagcag accagcagac tgatcttaca gaagatttca agggtcctgc
    21001 taagaagatt gccttggatg cagacgggaa cttcactaaa gctgcagaag gctttgtccg
    21061 tggcaaaggc ttgacgacag cggacatcga attccgcgag atcaagggtg aagagtatgt
    21121 ctacgtgacc aaacacgaag ctggtcagcc agctaaggca gtcttggcag gcattccaga
    21181 ggtcttgaag gctatgacct tccctgtcag catgaactgg gcgtccaaca aattcgccta
    21241 catccgtcct gtccacacct tgaccgttct tttggacgat gaagcattgg atatggactt
    21301 cttggacatt acatctggtc gcatcagccg tggtcaccgt ttccttggaa atgaaactga
    21361 gattgcaagt gcagattctt acgaggcaga cttgcgtgca cagtttgtta tcactgaccc
    21421 cgcagagcgt caaaatatga tcgtcgagca aatcaaggct atcgaggaca aacacaacgt
    21481 gacagttgaa attgatgaag atttgctcaa cgaagttctc aatctggtcg agtacccaac
    21541 tgccttcatg ggttcttttg acactaaata tttggaagta ccagaagaag ttttggtaac
    21601 ttccatgaaa aaccaccagc gttactttgt agtgcgggat aaggctggca agcttttgcc
    21661 aaacttcatc tccgtccgaa acggtaatga ccagtacctt gacaatgtca ttaaaggaaa
    21721 cgaaaaagtc ttggtggccc gtttggaaga tggtgagttc ttctggcgtg aggaccaaaa
    21781 actcaagatt gctgacttgg tagagcgact caaagttgtg accttccatg agaaaatcgg
    21841 ctcactttac gagcacatgg aacgcaccaa agttatcgca gaaaaactgg ctgacttggc
    21901 aggtttgtct gcgggtgaaa aagcagatgt ggcgcgtgcg gcggacatct acaaatttga
    21961 cctcttgaca ggcatggtcg gtgagtttga tgaattgcaa gggattatgg gtgagaagta
    22021 tgcccttctt gcaggggaaa aacctgcggt ggcagcggca atccgtgagc actacttgcc
    22081 aaactcagca gaaggcgaat tgcctgagag caaggtcggt gcggtcctgg cactggctga
    22141 taagtttgac accctcctat ccttcttctc agttggcttg attccgtctg gttcaaacga
    22201 cccttacgct cttcgtcgtg caacacaggg tatcgtgcgg attttggaag catttggttg
    22261 ggaaattcca ttggatcagt tgattgcgga gctctacagc ttgaattttg ctagcttgac
    22321 ctatgacaac cagccagctg ttatggactt tatccgtgct cgtgtagaga agatgatgga
    22381 caagaccatt ccgaaagaca tccgcgaggc tgtacttgcc agctcaactt ttgtggtcag
    22441 actgcaactg gcagccagct ctgctatttt ccaaaaagca aaagaggctg actacaagga
    22501 agccgtggaa aacttgtcac gtgtcttcaa cttggctgaa aaggcggagg tgactgcgat
    22561 tgacgaggcc ctcttcgaaa acgaccagga aaaagccctt gctgctgcag tggcaggctt
    22621 ggagttgaca gaagatatgg cgggcaactt ggacaagctc tttgctctca gcccagtcat
    22681 cgcggccttc ttcgacaata ccatggttat ggtagatgac gcagcagtca aagccaaccg
    22741 cttgaccctc ttgaaagcct tggcggataa ggcaagtgct gtggcggtct tcaatctctt
    22801 gaatagtaag taggaggcat tcatggaaca agcaaaaatc gatcggatca atgaactggc
    22861 ccgcaagaaa aaagcagaag gcttgacaga ggaggagttg caggaacagg cggctcttcg
    22921 tgaggagtac atcgagggct accgccgtag tgtgcgtgcc cacatcgaag gtatcaaggt
    22981 cgtcgatgaa gacggcaatg atgtgacgcc agagaaactc cgtcaagtcc aacgtgaaaa
    23041 aggcttgcac ggacgtagct tggacgatcc caattcctaa gagtaagatt gctaaaaaaa
    23101 caaggaaaga ctaggttttc tagtcttttt tgtgcttttg agaacatata tttacttgtt
    23161 cattcgattc ataaatggta aaatgtatat agataaatag gctcaggagg gctttgtcat
    23221 gaaaaaacta ctctattttc tcttgtttct tacctacatc gcatgtgcat acagcacgat
    23281 tttctttctg ccttttatgt acttttctgg aattgttggg gcaacaggag ttggtctggg
    23341 cctaagctat tccctacatc aagaagtcaa aaagcatcct gaaaaacaat tgtcagagta
    23401 tttaccgtgg ggatctaaga gggacaagat tatggtagta accattctac ttttctccat
    23461 tttggtcagt ttcattccag atgtgaattg gcatagaggc ctggcaatcc tcttagatat
    23521 cagctatgta ctcctttggc aagctatgct tatccaattc ttacgaaaat attattttaa
    23581 aaacgcatga gagtttcatg cgttttcttt tctaaatact ttgtccagct tttaattttt
    23641 tcataaagat gatggaactt ggaatcagga cgatgatgct tccgatccag gcggcggggt
    23701 tagcggcggc ggttccataa aaaccgaaat actgcatgcc gatgatggcg actgctgctc
    23761 ggaaaatcag ttctccaaag cctgctagtg ttgggataaa gcccttgcca agtccctgaa
    23821 taaagctacg aagaataaag agggaagcaa caatccagta acaagagccg ttaatgatgt
    23881 agtaaacaag agccaagtcc agcactgttt ggctggcatt tggcagaaag agaccagaga
    23941 agaagcgatt gcccaagatg agaatgaccg caaatacaat ggcccaagca atgctgagga
    24001 gtaatgagtg gcgcactcct tctcgaatac ggtcatagag cttggcaccg tagttctgag
    24061 cagtaaaggt tgcgatagcc aagcccagat tgaccatggg cagcatagcc aactggtcag
    24121 tacgaagggc aatggcctgg gcggcgatgg cgtctgttcc tagttggttg accataaact
    24181 gcaaggtcat ggctccgatg gcgataatac tggcttgaaa tcccattgga aaagcaagct
    24241 gggcatgttt cttgagatta cctctatcca attttaaatc agctagtccg atatgatagt
    24301 gaggtacctt gtggataatg taaaaaataa gaaagaggac cgaacaggct tgggcaagga
    24361 tagttgcaaa gccagctccg aaaacgcccc agttcataac taagataaag aaaaaatcca
    24421 gaccgatatt gataaaacaa gcgataacta gagccagtag aggtgtttta gagtttccga
    24481 gactgcgtag ggctgaagac aggaaattat aaaaaatagt aaaggtcaat cctccaaaca
    24541 tggccgttag aaaggcatgg gagtggtcga tgatactcac aggcgtttgc attaagacca
    24601 agataggttt caaaaaagct aaggctgaga ctgttagtaa aaggctgatg agggaagcgt
    24661 agaagagtcc gtggacaaaa gattttttaa gcccctctaa gtcgccagct ccaaagcgtt
    24721 gggcagaaat aatggttaat ccattggtaa caccttgggc aaaaccgatg attaaaaaaa
    24781 tcaggctggc tgtcgaaccg acacttgcaa aggcgtcctt tcccaaagta tgccccacaa
    24841 tcatggaatc agcaaaatta taggccagtt gaaaaaaaga tccaatcaac aagggaatag
    24901 cgaattgtaa gatgaccgca atcggctttc ctttggttaa atcattcata acgtctcctt
    24961 ttgacagatg ataagcccac tagataaatg atagtctagt aggctttcta gggataaaat
    25021 atttctcctg aaaacggagt ataggcgtaa atttgttatt tatactcttc gaaaatcaaa
    25081 atcagacgtt gttgacttga tttgatgagt gaaaggctcc agtggagcct ttcagcctgt
    25141 taccttgaaa caaaatagta acagggttct atctgcgtaa gtagtaacga actacgttcg
    25201 ccctatctcc aacctccaaa ggttccccaa acctttggag ctagtctgtt tttgattttc
    25261 attgagtatt aatttgtcag ttcttcaaaa tgatcgacag tgttatggtc tgttactgct
    25321 aagatgagtt cgtcagattt gaagacatag tcaggagtca gttggatgtt taattcctga
    25381 ttttcgccac ttcggtaacc gatgatattg agtttataat gttggcggag ttttaattcg
    25441 ccgagtgttt ttccaatcca ttttactggc gggtgaaact cgacaatgga aacattgcct
    25501 tcgagctcaa agaggctggt tgtatcgcgg tggagtagtt gttttgctag agaaatacct
    25561 gtttcacgtt caggagaaat aaccttatcg gcaccgacac gaagaaggac ttctttcgca
    25621 gttgttcctt tgactttgac aatgaccata ggaacaccta gaactttgct atgcatgaca
    25681 gccagaacac tggattccaa gttttcgcca gtagctacaa caacagcatc gcagttaccg
    25741 attcctgctg cacgtaggag cgagcgatcg gtgatgtctc cgacaatacc gcgtgtgaga
    25801 ataggttcta gatgattgat acgttgctca tgattatcga tagcaatgat attgcaatcg
    25861 taattgtgta atgtttttgc aatggtagta ccaaaaactc ccaggccgag aattccgata
    25921 gtatagtttg gcatgatttc tccttttaaa caagcataga tgattttgcg tattgaagac
    25981 tttcggtttt agataatttt cgaacagaaa ggcttgcgag aatagaaagt ggaccgatac
    26041 ggccgaagaa catgagcgcc ataatgactg cttgaccggc catggttagg cttggagtca
    26101 gatttgccgt aacacctaca gtggctaatg ctgacatgac ctcgaacatt aaaaatagaa
    26161 gtggttgtcg gtcatctgtt agggcaagtg cgaagagccc tatgataaac ataagtagga
    26221 agactgagaa tgtcgcaaaa gctttgcgga tgctgaggtt atcgatggtt cgtcccataa
    26281 agttggtatg agggagaccg agaatctcgc tacgagcata gagcactaag gttaggaagg
    26341 cggttatttt aataccgccg gctgttccac ctggggctcc gccaagcatc atctgaaaga
    26401 tatagagcaa gagggtgatt ggttcagcct tcgtatagtc gatgctggca aaaccagcgg
    26461 ttctcatggt gaccgtctgg aaaaagctgg ctaataattt atggccaaat gatagttgcc
    26521 cgatagtaga agggttattc cattcagtga gtaaggttgt cagagttccg agggttagta
    26581 taatggctgt taggcctaga acaatctttg tatggaaccc tagttttcgt aggctacgtt
    26641 tttgcccgat ttgtgtttga aggtcaaacc aaaccgagaa tccaagacct cccataataa
    26701 tgagactggc taatgttatg ttgattagag gattatccac atagcgcatg atgctagttg
    26761 cgccaaaatt atcaaaacca gcattacaaa aggctgatat agctacgaaa atggaggtaa
    26821 ataaacctct cgcccatcca aattcaggca caaaatgaaa ggacagtaaa agggctccaa
    26881 aggcttcgac cgccaaggta aaaccaaaag ctgatttgag gaagttacga aaatattttg
    26941 tgtcagatcg gtttagagct tcttgtaagg tcgccatatt gatgaaattc attttacgac
    27001 caccgcgtaa agcgaagaga ccgatgaatc ctatcaagct caaaccacca atttggatta
    27061 gcatcataca gataatctgt ccaaaagtag aataggtttc agcaacggct ttggtaaata
    27121 gccctgtcac acaaaccatg gagacagatg taaagagatg atcccaataa cttgctgtag
    27181 aggtgctaat ttgtgagatg ggaagactta gtaaaattga tccagtcaga atcacgaata
    27241 agaaactgat aatgattcgc tgactgggag aaagtttgat cctaaatggt gacataattc
    27301 taccttttct caaaaaaata aaagatgaag atattgtagc acaaaagaaa gaaaatgtac
    27361 gactaaaaca cgcaaagaac ctgatttttt tctcttcttg aaagcttcat tattttttat
    27421 taccatggat tttataagtg gtgtttatat gaggtggctc attatatttg tgacaccagg
    27481 attatgggga aattattatg ggcaatgaaa aagtggtgcg gaccataaaa agaatctcct
    27541 tagttgacct agggagattt atttccattc tgctaagact agttgatgat agtgacgtaa
    27601 aggtccgacc ttttggagga ggtaggtagc agccagtccg accaataagc ctgataaaat
    27661 tcctgtaaaa gcgaggattg gcaggtagtt tagtacatag aaagaacggg caatggtagc
    27721 aaaaacaaga agctgaccaa gattgtgcaa catcccgccc agaatagaaa ttccaatagg
    27781 actgacacgg tttggaccga gctgcttggc agagagcatt cctacataac tcaaagtagt
    27841 tccggcaaag ccatagagga atgtggaaaa ggtgcctccc agaaaggttg agatgagcaa
    27901 gcgtagagcg acaaccttta ggctatcctt tgtagggagg gtaaagatag caattaagct
    27961 aatcaaattt cccagtccta gcttggcgcc tggagcaaaa gcaaatgggg gggtgatgag
    28021 tcgttcaatc aaagaaatga caactgcctg agctgctaac atcgtaataa agttaagtgt
    28081 ttgacgttta gaattcatgg gtgtagagaa aaaactcttt ctagtcgatt atctatttta
    28141 ctatcatact tgtttttgtt cactatggca agttttgttt gtgtatatta ctaattattg
    28201 aaatttgctt gtcaactatc ttggaatacg atataatgga aacatgtaat ttgttttgat
    28261 tttcacaagg aggaaacatt gtgacgaaaa atattgtgat tgtcggtgct ggttatgcgg
    28321 ggattgcagc agctcgattg ctaggaaaaa cttttaaaaa ggatcaagat gtaacagtta
    28381 ccttgattga taagaactct ttccatacct atatgacaga gttgcatgaa gttgcggctg
    28441 ggcgtgtgga agccaatgca atcaagtatg accttcaacg tatcttcaaa aaatatccaa
    28501 aggttcaatt ggtaacagac aaggttgttg agattgacta cgataagaaa caagtagttg
    28561 cggaacacca aactctggat tttgattacc tgctccttgc aatgggtggt gaggctaatg
    28621 actttggtgt gaaaggtgtt aaagaacacg gttttacact ttggtctatc gaagcagcag
    28681 aacgtttgca tgaccacatg attgatgctt gctatcgtgc catgcgtgag catgatgaag
    28741 ccaaacgtcg cgctctttta acctttaccg ttatcggtgc tggcttcaca ggtattgaga
    28801 tgattggtga gttgattgac tgggtaccaa ttttggcgcg tgagttcaag ttggatccaa
    28861 aagaattctc acttaaggtt gttgaggcaa cgccaaatat cttggctatg gttactgaaa
    28921 aagagcaagt caaggctcgc aaataccttg agaaaaaagg tgttgaattg gttcttggcg
    28981 atggtgtagc aagtgtacaa gaagatagct tgacactttc atcaggtcgt caaattccta
    29041 cctatacctc tatttggaca gctggtgttc aagccaatac agacgcaagt gaatttggta
    29101 ttgaaaaagc acgtgcaggt cgtttggtag ccaatgagtt catggaagcg aaaggcaagg
    29161 aaaatgtcta tgtagctggc gacttggttt actttgaaga atcagaagga aaaccaactc
    29221 cacagattgt acaagcagct gaacagacag gccatactgc ggcaagaaat attatttcag
    29281 caatcaaggg tggcgaaaaa catagctaca aaggtaaata tgatgggttt atggtatcta
    29341 ttggagcacg ctacggtgta gccttcttga tggacaaata ccacatgtca ggctttatgg
    29401 caatggctgt caaacacatg gtaaacctac tctacttctt tactatccgt agtttcttct
    29461 acatgggttc ttatgttcgt catgaattct ttgacattca gaataagcga aacatttttg
    29521 gcggtcatac ttcaggcaaa ggaaatcttc tctggtctgt gccaatgcgt gtcctttatg
    29581 gttcagtatg gctctacgaa ggtatcaaga aagcctttgg tctatttgga acaacatcat
    29641 ggtttgggga tcaagttgtc ttcccattcc cttggttagc tgatccagta tctggtgcat
    29701 ctgctgctga ggcggtttca agtgcctcac aagcggcaac tgctgcggca gaagcagctg
    29761 aaccaatttt tggtttgagc tatgcctatg gtgaagcacc gatggctgtt ttggacaaaa
    29821 tgcctgattg gtttgctact atcatggaat ttatgatgcc aaaccaagaa gtagcactct
    29881 ttatgcaaaa atttatgaca gtagcagaaa tcggtattgg tctggctttg attgctggtg
    29941 cctttgtatg gattgtatct gctgcaaccg ttgctttggt agttatgttc agcctgtcag
    30001 gcatgttcta ctgggtaaat atttggttca tcccagcagc tatttctctt atgaatggtg
    30061 cgggtagagc cttcggtcta gattactgga ttatgccatg gttgggtcgt ttcctagaca
    30121 agaagattta tggaaagccg aagcatattt accgtatcaa gtataaaaaa taaaggaaat
    30181 atagaaagtt agtggtacat caaatttgga atacctatcc tgagattgag aggggacttg
    30241 aagaggtaaa atccatcatc ttatctgaga tgatggtcct gcatccagca gttacatcga
    30301 agattgttga atatgtagag gcgccaggga aatacctgcg tgctggcctc tgtctgctct
    30361 ttgcacaaat gacggaagga aagattagcc gttcaaagct atattttgct gcccatttag
    30421 aagtcctgca tctggcgact cttattcatg acgatgtgat tgatggcgcg gataaacggc
    30481 ggggagtcac ggcggctcat caacaatttt cgaatcgaat cgctatctac acgggtgatt
    30541 atcttctggc ctattcaagt cgactatttg gaaaggggct tcgtttgcta gaagtatcac
    30601 gtgatgattt ggatttgggc aatgataagc tgatggaaac aattcttcga ggggaattat
    30661 ctcagctact caatcaattt gatgccaata tgactatgaa agcctatctc aagcaaattc
    30721 aaggaaaaac agcttttcta tttggacttg cctgtcagtt ggggagcttt gtaccaggcc
    30781 aatctaggaa agacagtagc atggcttttc ggtctgggca agcacttggc atggcatttc
    30841 agctacgaga tgacttaatt gactatcagc tggatgtagc agaatcagga aaacctagat
    30901 tgcaagatat tcaaaacggt atctatactg caccgctact ttttgccatg cgagaagaca
    30961 agtctatcca gcagcaattg cgggcttata ttcaatcccc tacggcagaa gggttggaaa
    31021 tccttacaga taggattttt gcaacgcatg ctattgccaa aacagagaaa ctgattgtta
    31081 gctatttgga aaaaatgatg aattttgtag atagaatacc acagttatcg actgcgtcta
    31141 tgcaaaattt ggtggcaata gtttttagtc attattttta aaaaataact agacattttc
    31201 tctaattagg tatatcatta taagtataaa actatttact taacaaaagg agcattgtta
    31261 tgaaaacaac taaagttgtg ttgaaaagtt tggtagttct tggtgcggta tttgcactcg
    31321 cagcttgcgg gtctaaacaa gaatctgctt caacttcgtc ttcaactaaa gaaacagtag
    31381 tattgaaaga tggtacttat aaagctgagt caagcaaaga cgattatggc tacaaaattg
    31441 ttcacacatt gactgttaaa gatggaaaaa ttagtgagtc aaaatttgac tacgaagcag
    31501 aagacggaag tcttaaatca gcaaatgaag aatacaacaa aaaaatgaaa gataaagctg
    31561 gagtttctgc cggtgaagca attgaacaac tcaatgcagc tttggttgag aaacaagaac
    31621 tctcagctgt tgaagttgta tcaggtgcaa ctcatacttc tgaagacttc aagaagagta
    31681 cagaagccct tcttgcagca gctgctgaag gaaaaacaga aactgtaaaa cttgactaat
    31741 taagtctata cagaaaagac acagtcgcac ttgtttggct actgtgtctt cttatttttt
    31801 ggggaaggta aaatcagtga aaaaaattgc aagtctattg ttcgtgggcc tactgatggt
    31861 tttgcttgtt gcctgtggtg gtaaacaaaa cgaaagttta tctgttgtga aaactccgtt
    31921 gactcgtagt gaaagcttac ttcacacagt agtgcagttg agtatttatc atgaaaatca
    31981 ggaagaagcg atggatgaag ctgtctccta tattaaggag atggaaagtc tgttgtccac
    32041 aaacttggaa ggggcagatg tctaccgtat caatcaggca gcaggaaaag aagctgtcaa
    32101 ggttgatgaa cgtacctttg aagtgattga aacagctatt gacatgagta aagaaagtca
    32161 ggggctattt gacatttcca ttggagctgt gagcaatctg tggaaaattg gagatgagga
    32221 tgcacgtaag ccgagtgatg aagaaatcaa agctgctctg ccatttatca attataaaga
    32281 cattacttta gataaggaga agaagaccgt ctttatcaaa gaaggaatga ccttagaatt
    32341 aggagccatt tccaaaggtt acattgcaga taaggttagg gaattgtttg ccagcaaagg
    32401 gattacgact gctatcatca atctaggtgg gaatgtagtg gttatggggg attcgccaac
    32461 gacacaaaat ggttggaatg ttggagttca agacccagac caagtgcgtg gatcaacggt
    32521 cggttctgtg ccagggacac atgattcggt tgttacctca ggtatttatg agcgttactt
    32581 agaggtggac ggtgtcaaat atcaccatat ccttgatccc aaaactggat atcctgtgga
    32641 aaacgatatc tcaggtgtga cagtcttttc caaaacatct atccaaggag atgccttatc
    32701 cacaactctc ttcttactag gtgtggaaaa aggtcttgac tttatcaatc agattgacgg
    32761 tgtagaagct gttttcatcg ataaggagca gggtgttcat ctgagtgatg gcttgaaaga
    32821 aagctttgaa ttaacgaatg aggagtatca tcttgtcaat gaataaatca actaaagggc
    32881 tcagtttgcc tgtatttcta gaatttgttg aaatgaagac caaggtagcc agtgtttttc
    32941 cgatgaccct ggggatttta tggagcctgt atcgctatca agctttcaat tggctcaata
    33001 ctctcctctt cattattgcc gtccttagct ttgacatgtg tacaactgcc atcaacaata
    33061 gcatggacta ccacaaggcc aaggatgaag cctatcgtca ggaaagcaat gtgattggaa
    33121 aattctccct agattttcgt cagatgattg gaatcgtcct tgctctactt attttttctc
    33181 tactggtttc attactcctt gtatggcgga ccagttggct cctcctaccg atgggagctc
    33241 tctgtttcct cattggtatc ttctatacct ttggacctat tccgctttcg agaatgccac
    33301 tgggtgaagt tttctctgga gtaaccatgg gatttggcat cttctttctt gctgtattta
    33361 ttcagcaacc agatttgcta ctgaccagcc aagtgagcgg tcagtggatg actttgcaat
    33421 tttcttggat aaaaataatt gaaattattt tcatgtccat tcctctggta accttgattg
    33481 ctaatatcat gttggctaac aacacctgtg atttagaaga agatattcgc aaccatcgtt
    33541 atactctggt ttactatatt ggcaagaaaa atgctcttaa attgtatttt gtcttggcaa
    33601 gtttaccatg gttgctgtgg ataatctact gtctgatagg attcttacca atttgggcac
    33661 tggccggttt aatcggagtt tggcctgctt ataagagtct agaaacattt ttgaaaaagc
    33721 aagtcaaacg ggaaaccttt atcgaggcag taaagagttt tgtactcttt gcgctgctat
    33781 atgtggtgat acttgcttta gcccttattt tctaaaaagg aaagacacag tcgttcataa
    33841 ggctgtgtct tctttgttat atcgtcagtt agtttttctt ttattacttc gacgagcgag
    33901 gaaacatcgt ttcctaattt ccaacttcaa acactccact ggagtatttg aactcgcctt
    33961 gcctgattga aacagtctgg gggactgttt caagtcaatg cctagaaata agaaagcatt
    34021 gagttatgcc tgcggctttt gatgcgaact ttgttcgctt tatctccaac ctccaaagat
    34081 ttcccaaacc tttggagcta gtactaaaag taaactaaaa gactatagtg gtaaaatgag
    34141 ttcttcttca tcagtgcttt ctccgccaac ggatttgatt tcaatcaaca ggttatgtgg
    34201 caggcagacg gagagttgac ccggctggct aatccagctg gtcataacgg caatttggtc
    34261 tgggctattg tcttccttga cacgaatgcg ttcgccatca acttcaatga tattgtattg
    34321 cccttcattg ggatagtagg tcttttcttg atgaggtgtg tcctttgata attcaaattg
    34381 gtcgaccacc tcaccattga tgcggacata ggcaattgtt tttgcctcat tagtatctgt
    34441 agtcagatgt gtgtaggtaa aaattgctgg aagaaaggat aaaactaggg tgaatccgat
    34501 taagatataa tcaaatagtt tgagttgttt gagaatggat ttgaatgtca tatacaatct
    34561 attttataga aaaaatccta ctttgtaaaa tgttggatgc agactacttt ttcaattgtc
    34621 taaagaagag taggatggct aacattcccc aaaatccagc atgaagtggc tgaatttttc
    34681 cagcgaaaaa tacaatggag aaagttaggg caaaacctgc aaagagaaac cagagcagtg
    34741 gtttatgata aagatagata gctttttgga aagcatttgc ttctgggcca ctagtctgcc
    34801 agtcactatt caagattgga agcatagtga acagtaagat aaaaattggt ggattgtccc
    34861 cagtcaggta gctatgtata tacaggctac aaattgctgt ataggagaga acaatcaaag
    34921 tgagtatgaa tttcggtttg tcaacgttca tctggcttct cctttttgaa ataaatccga
    34981 taaagaatag cggcagcaaa tccagcagga aggatgagga taagtggttt gacatgtcct
    35041 aaccaaatgc ccacggaata ggtcaaggtc atggcaaaca gggcaaagat ggcttgaatg
    35101 ataaagtagc gtttttcttc tgctgtttga taggatttga tgatttggat ggtcggtagc
    35161 atgagcagga atgaaccgtt aatcggctca ttgaccatgt aagagtagat gaagaaggca
    35221 gaaaataaag cataaaatag aacaaaaaat cgacaggttt gtttgggtgt aaaaggttta
    35281 ttcatggggt cagctcctta ttttttcttg acagaatctg cttccagttt ttgcagatag
    35341 gcaatggcag acagggctag actggcaaag aggcccatca tgccaccgaa gaggagggat
    35401 tttacgtgga agatggaagc cagtagggga agctggaggc taaagaggtc tgccagccca
    35461 gcaaagaggc tagatagcag ggcaactccc agtcccaaaa ccagtggatt gagggtctgt
    35521 ccttgttttt cctcagggct cagttcgtcc atgtcatatt gaggcaggtc agcatggagg
    35581 gtgcgcatat tatagttaaa tgaaacaaaa acagtacatt tatgatataa tgtatttatg
    35641 gcatattcat tagattttcg taaaaaagtt ctcgcatact gtgagaaaac cggcagtatt
    35701 actgaagcat cagctatttt ccaagtttca cgtaacacta tctatcaatg gctaaaatta
    35761 aaagagaaaa ccggcgagct tcatcaccaa gttaaaggaa ccaagccaag aaaagtggat
    35821 agagataaat taaagaatta tcttgaaact catccagatg cttatttgac tgaaatagct
    35881 tctgaatttg actgtcatcc aacagctatt cattacgccc tcaaagct
//
LOCUS       DAZQCJ010000009        34022 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000009 DAZQCJ010000000
VERSION     DAZQCJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34022
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.009"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..404
                     /locus_tag="ACHU66_000426"
     CDS             <1..404
                     /locus_tag="ACHU66_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR3388738.1"
                     /translation="ELPVDEAAFIALHFVNANLENIFQESYRITGIIMKIEQIIQDYY
                     STEFNQDSIDYYRFITHVKLFAHRLVEGNEYNDEDDVDLLELMKKKYPREYQCGTRVA
                     DFIRLEYDYLLSPSELVYLIAHIRRLTKNLS"
     gene            415..2256
                     /locus_tag="ACHU66_000427"
     CDS             415..2256
                     /locus_tag="ACHU66_000427"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFR3388739.1"
                     /translation="MKYKNTALAILEAVGGEKNVLRATHCVTRLRLELKDENIVSDER
                     VKSISGVLGIMKKNGQYQIILGNDVANYYKEFTALGKFDSDSVQQVKKANVLEQVIEY
                     IAGSMTPLIPAMLGGGMIKVLVIVLPMLGLLKADSQSISFLAFFGDAPYHFMPIFLAY
                     SASQKLKVTPALAMSVAGILLHPNFVQMVSSGDPLHFLGAPVTPASYGSSVIPILIMV
                     WLMKYIEAVFNKVTPAITKSFLQPTLVLLVSGFIALVLVGPLGVIVGEGLSQLVEQMH
                     GVAGWLTLAVLGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVAFKSKNSNTKQIAFAAGFSALFAGVTEPALYGVTLKYKKPLYAAMIGGGLAGLFV
                     GLTGVKAYLFAVPSLIALPQFIYSEAASNITNAMIAAAISIIVTFILAYFLGIDEETS
                     TVNLEKVAPGISSRKNVFSPLSGQILPLEKVNDATFSKKMLGEGVAIIPKDGKVYAPF
                     DGAITSLFPTKHAIGLTSDEGVELLIHFGLETVELKGRGFVSHVSDGEKVEKGQLMLE
                     VDVEMLVAEGYDIVTPVVVTNTQEYLDVLLLSTKEEVNYADDLLAVL"
     gene            2271..3695
                     /locus_tag="ACHU66_000428"
     CDS             2271..3695
                     /locus_tag="ACHU66_000428"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFR3388740.1"
                     /translation="MSIFPDNFLWGGAVAANQVEGAYNEGGKGLSVQDVLPNGGLGEA
                     TQEPTEDNLKLQAIDFYHRYKEDIALFSELGFKVFRTSIAWSRIFPNGDEEFPNEEGL
                     QFYDELFDELLKNGIEPLVTLSHYETPLYLARKYHGWVDRKMIQFFEKFARTVFERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDLPNEELSKQDLYQAIHHELVASSLVTKIAHEI
                     IPDCKVGCMVLAMPTYPMTPNPEDVWAAHAYENLNYLFSDIHVRGYYPNYAKRYFKEN
                     GIKITFMEGDEELLKNNTVDFLSFSYYMSITQAADPSKYSSGRGNILGGLVNPYLEAS
                     DWGWQIDPIGLRLTLNRYYDRYQIPLFIVENGLGAKDQLVKDEAGGLTVHDDYRIQYM
                     QEHLLQVAEAIQDGVEVMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGSGSLTRY
                     RKKSFYWYKEVIQSNGSILGNSSK"
     gene            4452..5453
                     /gene="trpX"
                     /locus_tag="ACHU66_000429"
     CDS             4452..5453
                     /gene="trpX"
                     /locus_tag="ACHU66_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR3388741.1"
                     /translation="MLKNKNLLATIVALTVMVVAALFLTQKEQNNSTNSTEKVKIGVL
                     QFVTHDSLDEIYKGIKAGLEEGGYTTTDNLEIDFMNAEGDQSQVQTMSKKLVDNGNEL
                     LIGIATPAAQSLANATTELPIIMGAVTDPVGANLVTDLKNPGGNITGVSDQTPVADAV
                     SLIKEITPDAKTIGVLYSSNEDNSKIQVAEFKAAAEEAGYTVLEYAVASSNEIAATVE
                     VASSKADVLFTPVDNTVASAFSTVVSVANKTKTPIFTSVEDMVEGGGIASVTLSQYDL
                     GVATGKMAAKILDGANPADTPVQIFNEGTVVVNQKVAKELGITLSDDVISKASKVIE"
     gene            5476..6342
                     /locus_tag="ACHU66_000430"
     CDS             5476..6342
                     /locus_tag="ACHU66_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002902146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR3388742.1"
                     /translation="MIVSTVSQALLWAVLGLGIFVTFRILNFPDMTTEGSFPLGGAVA
                     VTLLTQGVHPILATLAAVLVGCLAGFVTGLLYTKGKIPTLLAGILVMSSCHSVILFIM
                     GRANLGLLNTKRLQDFLPFSEQVNGLLLGLGSLAVVIGALMFFLYTRFGQAFIATGDN
                     PDMARSFGINTSRMELLGLVLSNGIIALAGALIAQQEGYADVSRGIGVIVVGMASLII
                     GEVIYGNLTMLERFLAIVVGAIFYQFLILVVISLGINTSYLRIFSAMILATCLMVPEL
                     KNKLLKGASLSR"
     gene            6339..7097
                     /locus_tag="ACHU66_000431"
     CDS             6339..7097
                     /locus_tag="ACHU66_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3388743.1"
                     /translation="MKAIVELKNATKVVTNGFDEERVILDNVNLTIYQGDFITILGGN
                     GAGKSTLFNVIAGTLPLTSGSVHILGEDVTNWPAEKRAKYLARVFQDPKMGTAPRMTV
                     AENLLIAKYRGEGRGLVPRRLAQQTEEFAQLLPRVGNGLEKHLDTPAGLLSGGQRQAL
                     SLLMASLKQPELLLLDEHTAALDPKTSQSLMQLTDELIETKGMTALMVTHQMEDALRH
                     GNRLIVMKNGQIIQDQTAEEKSKMKLEDYYKLFD"
     gene            7280..7765
                     /locus_tag="ACHU66_000432"
     CDS             7280..7765
                     /locus_tag="ACHU66_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFR3388744.1"
                     /translation="MDKLVFESLADLGMDYIVVEHPPALTTEEADRYIEGLEGVRTKS
                     MFLTNKKKTAFYLLIMDDQKQLDMDRFRDLVGANRIRMASSDSLMEKMHLPAGAVSVF
                     GLLHNVDKDIQVFFDKEILSEPILTFHPNVNTKTIFVKTEDVLRFVEEIGFSAKIVDL
                     G"
     gene            7771..8175
                     /locus_tag="ACHU66_000433"
     CDS             7771..8175
                     /locus_tag="ACHU66_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR3388745.1"
                     /translation="MRKCLAICSLLCCLFLVGCRTGAAYQAEIEPMEQSELYTEKEIE
                     AAILVVQDYFDHHFNSCKLLTIGYSGDNEKEFDEWAEHYGAEEAIILTSSFKVAAEGA
                     EPTLEPNSTHTDWKWILVRNVGGKWEHKGHGY"
     gene            8431..10092
                     /locus_tag="ACHU66_000434"
     CDS             8431..10092
                     /locus_tag="ACHU66_000434"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFR3388746.1"
                     /translation="MSTIKIMALGGVRENGKNLYIAEVNEHIFVLDAGAKYPENEQLG
                     VDVVVPNFDYLEENKHRVAGVFLSHGHADAIGALPYLLEKVKVPVFGSHLTIELAKLL
                     VKGNNATKKFNDFHVINAETEIDFGDSVVSFFQTTHSIPESLGIVVKTDEGNIVYTGD
                     FKFDQAADKFYRTDFGRLAEIGNEGVLALLSDSANADSNVQVASMHEAGEEILNTIAD
                     CDGRVIVAAVASNIVRIQQIFDAAEATGRRVVLTGHDVENIVRTAIQLKKLRLVSERL
                     LVKPKDMNKYEDHELIILETGRMGEPLNGLRKMSIGRHRYVEIKEGDLVYIVTTPSIS
                     KEAVVARVENLIYKAGGTVKSITKNLRVSGHGNARDLQLMLNIMKPKYLFPVQGEYRQ
                     LDAHAKAALEIGMYPENIIIVKRGDVMAFEEGDFVHSGAVPAGDVMIDGNAMGDVGNI
                     VLRDRKILSEDGIFIVAITVNRREKKIISKAKVNTRGFVYVKKSKDILREASELVNTT
                     VENYFTKDSFDWAELKSAVRDDLAKFLFDQTKRRPAILPVIMEVK"
     gene            10157..10933
                     /locus_tag="ACHU66_000435"
     CDS             10157..10933
                     /locus_tag="ACHU66_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR3388747.1"
                     /translation="MAIMKIEYHSEALDMSRQVTVLYPDRNRVENPDDNDIPVLYLLH
                     GMGGNQDSWLNRSTVERLVRYTNLIVVMVNTDKGWYTNTTYGMNYFDAIAVELPQILK
                     RFFPNMSDKREKNFIAGLSMGGYGTFKIAMMTNRFSHAASLSGALYFDFDNPAAAELG
                     SLSYWQGVFGDISDKQNPNNLLEIAKQSDKKTKFYAWCGEEDFLFEGHQKAVGELKEL
                     GFDIEASFGPGKHEWYYWNQQIEKVLAWLPIDFKLEERLS"
     gene            10999..11694
                     /locus_tag="ACHU66_000436"
     CDS             10999..11694
                     /locus_tag="ACHU66_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HFR3388748.1"
                     /translation="MKLIGNILRWIWRTFWGLIWLSMMLLACGLGLLFYFQQEAAPQM
                     LQTLAQEVQLMVKGQSEVTIEEGVNTIKHLTTDTVNTADHGRWESNTATVYIETQNPT
                     FVAAYETAIANWNTTGAFTFVMTSDPNQADIIATEMNDGNTQAAGEANSTTNLLTNYY
                     SSVTVRLNSFYLLNDQYGYDMDRIIHTAEHELGHAIGLDHEDSQTSVMESAGSNHGIQ
                     QADIDAVLALYSE"
     gene            11705..12070
                     /locus_tag="ACHU66_000437"
     CDS             11705..12070
                     /locus_tag="ACHU66_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388749.1"
                     /translation="MLRTITNTIKRYPEQALLFLYNAGIFAWMQSTSHSIMEQIGIDS
                     NWFDKIPEPIKAWTGASLESMQTLLNSSAWGWLIVSMILMLVIRFVKGLIKFVIMLII
                     IGGGLYLLWQNKELVQSLV"
     gene            12121..14019
                     /locus_tag="ACHU66_000438"
     CDS             12121..14019
                     /locus_tag="ACHU66_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFR3388750.1"
                     /translation="MIILSGNKIERSFAGEVLFNNINIQVDERDRIALVGKNGAGKST
                     LLKILVGEETATSGEISTKRDLSLSYLAQDSRFESANTIYDEMLHVFDDLRTTEKRLR
                     SMEEQMGSLSGSDLDQLMKTYDSLSEEFRLAGGFSYEADIRAILNGFKFDQTMWDMKI
                     SELSGGQNTRLALAKMLLESPELLVLDEPTNHLDIDTIAWLENYLVHYKGALIIVSHD
                     RYFLDKVATLTLDLTKHSLDRYVGNYSQFVELKEQKLQTELQNYEKQQKEIAKLEDFV
                     QKNIVRASTTKRAQARRKQLEKMERLDKPTTGQKSANMTFQSDKTSGNIVLTVENAAV
                     GYDGDILSQPISIDQRKLDAIAIVGPNGIGKTTLLKSIIGALPFIKGEAKLGANVEVG
                     YYDQTQSALTPSNTVLEELWSAFPTTPEVEIRNRLGAFLFSGDDVKKSVAMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     KVLEISETGSTLYLGDYDYYLEKKAELEVEAQPDQLESTSQSAGAMDYQAQKENQKEQ
                     RKLARRIEQIEAEIETIENRLSELNQAMLETNDIGQLTDYQKEIDQLTSQQESLMEEW
                     EELSEQMG"
     gene            14028..14357
                     /locus_tag="ACHU66_000439"
     CDS             14028..14357
                     /locus_tag="ACHU66_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388751.1"
                     /translation="MIQQNTETLEDNLRFNKLSKLIQTKLSQYFETYPSAVQCQLVCF
                     DRDETRRKVLSYDIYSDFDKLFWVESIAKPISSYSYGHQIDKKDIDKLDREFILDTFL
                     GIDEERG"
     gene            14364..15191
                     /locus_tag="ACHU66_000440"
     CDS             14364..15191
                     /locus_tag="ACHU66_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA methyltransferase"
                     /protein_id="HFR3388752.1"
                     /translation="MTKHQRFLNSDQFFACPHCGQALGLELNSLRCPNRHTFDIAKQG
                     YVNLAPQVKQSANYHKSSFENRQAFLEAGYYDHLYEALERKIAELGLRSVLDIGCGEG
                     FYSRKLAEKMDLDILAFDISKDSILLAAKSDRTKSVKWFVSDLTKLPIQDKTIDGILD
                     IFSPANYQEFARVLKAGGAILKLVPGPNHLKELRHLAKDQLRKESYDNQDIVDHFKSY
                     LGQVEQVLVSRTLPITAAHAQVLADMTPLFFQVDQSKLDLSQLTEITIEGVLLIGTI"
     gene            complement(15231..16016)
                     /gene="gatD"
                     /locus_tag="ACHU66_000441"
     CDS             complement(15231..16016)
                     /gene="gatD"
                     /locus_tag="ACHU66_000441"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit GatD"
                     /protein_id="HFR3388753.1"
                     /translation="MVYTSLKSPEKDYTYDLHIAHLYGDLMNTYGDNGNILMLKYVAE
                     KLGARVQVDIVSLTDEFDKNFYDIAFFGGGQDYEQSILAKDLPTKKDSLADFIENDGV
                     MLAICGGFQLLGQYYIEAGGRKIDGLGIMGHYTLNQTNNRYIGDIKIHNDEFNETYYG
                     FENHQGRTFLADNQKPLGKVVYGNGNNKEDGGEGLHYKNTFGSYFHGPILSRNANLAY
                     RLVTTALRKKYGQDIQLASYADILSKEVAEEYGDVKSKAEFEK"
     gene            complement(16016..17359)
                     /gene="murT"
                     /locus_tag="ACHU66_000442"
     CDS             complement(16016..17359)
                     /gene="murT"
                     /locus_tag="ACHU66_000442"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HFR3388754.1"
                     /translation="MKVNTALGILAGKSSQFILKKLGRGTTLPGKIALKFDKHILDSL
                     AKDYEVVVITGTNGKTLTTALTVGILQEAFGEITTNTSGANMITGITATFLSAKKNKK
                     GKKIAVLEIDEASLARVTDFIKPSLIVFTNIFRDQMDRYGEIYTTYQMILDGAAKAPQ
                     ATILANGDSPLFNSTNVINPVKYYGFATEEHEPRLAHYNTEGVLCPHCHQIIQYKLNT
                     YANLGSYICNNCGFCRPELDYKLTDLKEITNTSSTFAIDGQDYKINIGGLYNIYNALA
                     AVSVAAFFGVTPEKIKAGFDKSKAVFGRQETFKLGDKDCTLVLIKNPVGATQAIEMMK
                     LAPYDFSLSVLLNANYADGIDTSWIWDADFEQITQMDIPQVFAGGVRSSEIARRLRVT
                     GYPENQITETPKLEDIMALIEQSSSQHAYILATYTAMLEFRDLLAQRQAVGKEMK"
     gene            17541..18395
                     /gene="cdaA"
                     /locus_tag="ACHU66_000443"
     CDS             17541..18395
                     /gene="cdaA"
                     /locus_tag="ACHU66_000443"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFR3388755.1"
                     /translation="MLNLNQLLDTGYWSSLIASPWTALLHLIDISIVVYLIYNFSKAI
                     AGTKIMTLIRGVFLFIIVQIIASLFGLQTIAWLINQVITYGVIAAVVIFAPELRAMLE
                     KLGRTTQIFGTNTVSAEEKLIVAFLKSVAYMSPRKIGALVAVEQAQTLQEYRATGIPL
                     NADISQELLINIFIPNTPLHDGAVIVKEDKVAVACAYLPLSESAGISKEFGTRHRAAI
                     GLSEVSDAFVFIVSEETGSISIARNGIFKHDLTLDEFEAELRATFISENVEKKSIWKR
                     LGGGKHER"
     gene            18385..19089
                     /locus_tag="ACHU66_000444"
     CDS             18385..19089
                     /locus_tag="ACHU66_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFR3388756.1"
                     /translation="MNDKFKAVGHLALSIFLALLLFFYATTTNYKNSESAAQNTESET
                     YVHTLSNVPIEIEYDTNKYFISGLSSTVAVELRGSNRVLLQRESDESTRTFQVTADLR
                     ELSDGTQTVKLQLTNLPAGVSATLAPDAITVKIGKKVSKAFPVAGRVYSNQLAEGYSL
                     SKVSVNVDTVKVTTDEETMAKIDRVEAVAMDVSNLSENYSGTAKLQAVDSDGNVLPVV
                     LSQTEANMQIILTKTK"
     gene            19102..20451
                     /gene="glmM"
                     /locus_tag="ACHU66_000445"
     CDS             19102..20451
                     /gene="glmM"
                     /locus_tag="ACHU66_000445"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR3388757.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETDVPRV
                     FVARDTRISGQMLEAALIAGLLSVGIHVYKLGVLATPGVAHLVKTEKASAGVMISASH
                     NPAQDNGIKFFAGDGFKLDDALEAEIEALLDAEEDTLPRPSAQGLGDVVEYPEGLRKY
                     QQFLVSTGTDLDGMKVALDTANGAAATSARQIFVDLGADLTVMAEKPDGLNINEGVGS
                     THPEKLQELVKETGSQIGLAFDGDSDRLIAVDENGDLVDGDRIMYIVGKYLADRGLLA
                     KNTIVTTVMSNLGFHKALDREGIEKAVTAVGDRYVVEEMRKEGYNVGGEQSGHVILMD
                     YNTTGDGQLTAVQLTKIMKETGKKLSELAAEVTIYPQKLVNIRVENSMKDKAMEVPAI
                     AAIIEKMEAEMAGNGRILVRPSGTEPLLRVMAEAPTDAEVDYYVDTIADVVRAEIGI"
     gene            20597..22141
                     /locus_tag="ACHU66_000446"
     CDS             20597..22141
                     /locus_tag="ACHU66_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFR3388758.1"
                     /translation="MSILEVKNLSHGFGDRAIFENVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGQLQPDEGKVEWSRYVTAGYLDQHAKLEKGQSVRDVLRTAFDELFKTEARIN
                     DIYMSMAEEGADMDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDYGMDKDVT
                     ELSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQLLTRYTGDYYQFQEVHAMKRAQLEAAYERQQKEIADLQDFVN
                     RNKARVATRNMAMSRQKKLDKMEIIELQAEKPKPSFDFKMARTPSRFIFQTTDLEIGY
                     DRVLTRKPLNLTFERNQKIAIVGANGIGKSTLLKSLLGIIPPLGGSVERGEYLELGYF
                     EQEVAGGNRQTPLEAVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQAKVR
                     FCLLMNRENNVLVLDEPTNHLDVDAKDELKRALQAYKGSILMVCHEPDFYEGWVDDVW
                     DFNELT"
     gene            22363..23016
                     /locus_tag="ACHU66_000447"
                     /pseudo
     CDS             22363..23016
                     /locus_tag="ACHU66_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775244.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            23179..24384
                     /gene="sstT"
                     /locus_tag="ACHU66_000448"
     CDS             23179..24384
                     /gene="sstT"
                     /locus_tag="ACHU66_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR3388759.1"
                     /translation="MNKIISVWKKMNLIRKIGIGVVLGVLLGLIAPKITVIALFGSLF
                     VGALKAIAPLLVLTLVAHALSQAPAGQKSNMRTVICLYLFGTFAAAFIAVGASYLFPI
                     KLVLSTTTTTDIIPPQGIAEVFQDLLLKVVDNPINALATANYIGVLTWAAVFGLAFRH
                     ASKTTKDLLQSAAEVTSKVVGWIIGLAPFGIMGLVFDTIANNGLTALKDYGLLLLLLV
                     GSMIFVALVVNPLIAFLVMKKNPYPLVFECIRVSGVTAFFTRSSAANIPVNMQLCKRL
                     GVDPDTYSVSIPLGATINMAGAAITINILTMAAVHTLGISVDFSSALLLSVVASLSAA
                     GASGVAGGSLLLIPVACSLFGIPNELAMQVVGVGFVVGVIQDSCETALNSSTDVLFTV
                     VAERSAWKK"
     gene            24646..26046
                     /gene="pepV"
                     /locus_tag="ACHU66_000449"
     CDS             24646..26046
                     /gene="pepV"
                     /locus_tag="ACHU66_000449"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFR3388760.1"
                     /translation="MTVDFRAEFDKRKDEFLADLFDLLRINSERDDSQADAQHPFGPG
                     PVRALDKFLEIAQRDGYPTKNVDNYAGHFEFGEGDEVLGIFGHLDVVPAGSGWDTDPY
                     EPQIIDGRLFARGSSDDKGPTMACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADMD
                     YYFENVGLPLPDFGFSPDAEFPIINGEKGNITAYLHFAGENSGAAKLHSFTGGLRENM
                     VPESATAIISGDLADLDSKLADFTAAYGLKADAETLENGQIQVTVIGKSAHGSTPEEG
                     VNGATYLAKFLSQFAFDGAAKAYLDLAGQVLLEDHDAQKLGVAIYDEQMGALSMNAGV
                     FKFDETSSDNTIALNFRYPKNTNPETIKAGLEKLGVEAVSLSEHGHTPHYCPIDDPMV
                     ATLLSVYEKHTGLKGHEQVIGGGTFGRLLKRGVAYGAMFPGDVNTMHQANEFIEVEQL
                     YRAAAIYAEAIYELIK"
     gene            26087..26545
                     /locus_tag="ACHU66_000450"
     CDS             26087..26545
                     /locus_tag="ACHU66_000450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR3388761.1"
                     /translation="MKLKELTVADLEMVKQLFLSVFSQEPWNDDWSDEEQLDCYIGDL
                     LGHPRALCFGLFDPEKVIGLSLGYIRYWCEGTEYRIEEFCISRDYQGQGHGASLLADI
                     EKQLADRKIVHILLQTERNLSAYSFYKKCGFRALEEDVTMVKKVGHHGTC"
     gene            26532..27110
                     /locus_tag="ACHU66_000451"
     CDS             26532..27110
                     /locus_tag="ACHU66_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFR3388762.1"
                     /translation="MELADIRQAIIEDVANRDFTNKGIAPLFQAPSTAKILIIGQAPG
                     LKTQEKGRLFDDASGNNLRQWLGVDRQTFYESGHFAILPMDFYYPGKGKSGDLPPRKD
                     FAPKWHPLIMKQLPQIELTILVGNYAQTYYLEKSQRNLTERVKHADDYLPKYFPLPHP
                     SPRNNIWQAKHPWFVEQTLPILKNNINILLGK"
     gene            27182..27964
                     /locus_tag="ACHU66_000452"
     CDS             27182..27964
                     /locus_tag="ACHU66_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFR3388763.1"
                     /translation="MRKWKKIIIGCFAILTVCLLFAPTLARANQIPDRPTGTTVVDET
                     QLLSSETIAEIDQLNRSWASTEQALQVGVYVTESLSSDIESLANETFRHWQVGFAGTD
                     NGVLLVVAIADRAFRIETSDNAATVITDVEAKDILENAREFFRQEDYSGGISYIVNSI
                     GDRFYGTSIGKNQLAAIEEGTSEKDDGFWLFLIVILIAILFGIIDKSSRGGGGPGNLL
                     WMLVDDHHHYHNHHSSNSSSSSFGGGSWSGGGGGGGGASSGW"
     gene            28037..28618
                     /locus_tag="ACHU66_000453"
     CDS             28037..28618
                     /locus_tag="ACHU66_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFR3388764.1"
                     /translation="MKKKWLAILLPILALLFLGMGAVGQYNGLVDSYAEVENAQANVD
                     TQLQRRYDLIPNVVAAVKGAMEHEEEIFTAIAEARAKIGSSQVGTSDYNQAQSQLDSA
                     VSRLLVVAENYPQLTANQQVSALITELEGTENRILVARKDYNSVATTYNKKIKRFPTS
                     IYANLFGYEKVELFQATNDAATTVPSVDLSDKE"
     gene            28630..30093
                     /locus_tag="ACHU66_000454"
     CDS             28630..30093
                     /locus_tag="ACHU66_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR3388765.1"
                     /translation="MDFGFDILAIMIPIIVVVLFVLLFVFGYVSAKPNEAIVITGLGK
                     PRTLIGRSGFMIPFIEKRSYISIEQFSTDVQTTDFVPTLDFINVKADAVVKVKVGISD
                     ELLNAAAQNFLNWKTADISASIQDVLEGNLREIIGQMELRDMVNNRQAFAEKVQSNAA
                     PDLAKMGLEIIAFTVQSFTDDNDVIKNLGIDNIVTIQKDAANARAKAEREQAEVRARE
                     DKAANDARVAADLEIAKKQNELAIEQANLKRQSDVQLAQANAAYGIEEQAQRKEIERA
                     TAEANIVKQQKEAEVKAEEVKVREQELSATIRKQAEAEKYARQQAAEADLIERQRKAE
                     AELYETQREAEAQKARAEAAKYAAEQEAAGIEAKGRAEAEAIRLKLEAEAEGLSKKAD
                     AMAKFNDAAVTEMVVNVLPEIAKNIAAPLGNVDKITMYGEGNASKMVGDLMTTMDKTT
                     EGLGLNVRDLISATLTGRAMSTGLLTAQETKEAEGDK"
     gene            30182..30490
                     /locus_tag="ACHU66_000455"
     CDS             30182..30490
                     /locus_tag="ACHU66_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388766.1"
                     /translation="MKMFRNSKKSKLFIQKINELLSDSELKLSKALKFQLLEAMELCE
                     KGSKISYLSYKIYPWVLEELALNRIQSDKLKMFKRYLEQERWKYYFGSALGMAFTSIR
                     "
     gene            complement(30612..31687)
                     /locus_tag="ACHU66_000456"
                     /pseudo
     CDS             complement(30612..31687)
                     /locus_tag="ACHU66_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647074.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(31799..33172)
                     /gene="mnmE"
                     /locus_tag="ACHU66_000457"
     CDS             complement(31799..33172)
                     /gene="mnmE"
                     /locus_tag="ACHU66_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR3388767.1"
                     /translation="MITKEFDTITAISTPLGEGAIGIVRLSGTDAFAIASKVFKGKDL
                     ATVPSHSLNYGHIIDPATGQVLDEVILGVMRSPKTFTREDVIEINTHGGIAVTNEILQ
                     LLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDVIRAKTDKAMHNAVRQLDGSLSQ
                     LINDTRQEILNTLAQVEVNIDYPEYDDVEEATTELVREKTLQFQALLENLLRTARRGK
                     ILREGIATAIIGRPNVGKSSLLNNLLREEKAIVTDIAGTTRDVIEEYVNIKGVPLKLI
                     DTAGIRETDDIVEKIGVERSKKALEEADLILLVLNASEPLTEQDRNLLAISDLANRIV
                     LLNKTDLEEQIEADQLPEDVIRISVLKNQNIDQIEEKINQLFFENAGLVEQDATYLSN
                     SRHISLIEQAVQSLQAVNDGLEMGMPVDLLQVDLTRCWQILGEITGDAAPDELITQLF
                     SQFCLGK"
BASE COUNT         9729 a         6470 c         8065 g         9758 t
ORIGIN      
        1 tcgaactccc agttgatgaa gcagccttca tcgctttgca ttttgtcaat gcgaatttgg
       61 aaaatatttt tcaagaatct tatcgtatta ctggaattat catgaagatt gaacaaatta
      121 ttcaagacta ttattctaca gaattcaatc aggattctat tgattactac cgattcatca
      181 cacatgttaa attgtttgct catcgtttgg tagaggggaa tgaatacaat gatgaagatg
      241 atgtggatct tttagagtta atgaaaaaga agtatccaag agagtaccaa tgtggaactc
      301 gagtagcgga ctttattcgg ttagaatatg attatctact gagtccaagt gaattagtct
      361 atttaattgc tcacattcga cgtttaacaa aaaatttatc ataaggagtc tattatgaag
      421 tacaagaata ctgcattggc aatcctagag gcagtcggag gagaaaagaa tgttcttcgt
      481 gcaacccatt gcgttactag attgcgactg gaattgaaag atgagaatat cgtgtccgat
      541 gaacgtgtta aatccatttc tggagtactc ggtattatga agaaaaatgg acagtatcaa
      601 attatcttag gtaatgatgt agctaattac tataaagaat ttactgcatt aggaaaattt
      661 gattctgatt cagttcaaca ggttaaaaaa gcaaatgtct tagaacaagt catcgagtat
      721 atcgctggct ctatgacacc acttattcca gctatgcttg gtggaggtat gataaaagtt
      781 ctagtcattg tcttaccgat gctggggctt ttaaaagcgg attcccaatc tatttcattt
      841 ttagcctttt ttggagatgc accatatcac tttatgccga tttttttggc ctactcagct
      901 tcacaaaaac ttaaagttac acctgcttta gccatgtcgg ttgctggtat tttgctacat
      961 cctaattttg ttcaaatggt atcttcaggt gatccacttc attttttagg tgcccctgtt
     1021 acacctgcta gctatggttc atcagttatt ccaattctta ttatggtttg gctaatgaag
     1081 tatattgaag ctgttttcaa taaggtcact ccagcaataa caaaaagttt cttacagcca
     1141 accttggtcc tgcttgtttc aggtttcata gctttagttc ttgttggacc tctcggcgtt
     1201 attgttggtg aaggattatc gcaattggtt gaacaaatgc atggtgtagc tggttggtta
     1261 actttagcag ttcttggagc aatcatgcca tttatcgtta tgaccggtat gcactgggct
     1321 tttgcaccga ttttcttggc agcttcaatc gcgacacctg atgtactgat tctaccagcg
     1381 atgttaggtt ctaacttggc acaaggtgcc gcttctatgg cagttgcatt taagagtaaa
     1441 aatagtaaca cgaaacaaat agcctttgca gctggatttt cagctttatt tgctggtgtc
     1501 actgagcctg ctttatatgg tgttactcta aagtataaaa aaccattgta tgcagcaatg
     1561 attggtggtg gtctagcagg attatttgtt ggtttgacag gtgtaaaagc ataccttttt
     1621 gcagtacctt ctctcattgc tttgccacaa tttatttact ctgaagcagc ttcaaacatt
     1681 actaacgcta tgattgctgc ggcaatttca attattgtca catttatttt ggcctatttc
     1741 ctaggtatcg atgaagaaac gtcaacagtt aatttagaaa aagtagcacc tggcatttca
     1801 agtagaaaaa atgtcttctc tcctctttca ggtcaaattc tccctttaga aaaagtaaat
     1861 gatgcaacgt tttcaaaaaa aatgcttgga gaaggagttg caattatccc taaggatgga
     1921 aaagtctatg ctccatttga cggggctatt accagtcttt ttccaacaaa acatgcaatc
     1981 ggtttaacaa gtgatgaagg ggtagagttg cttatccact ttggattaga aactgtcgag
     2041 ctaaaaggaa gaggttttgt aagccatgtt tcagatgggg aaaaagttga aaagggtcaa
     2101 ctgatgctgg aagtggatgt agaaatgctt gtcgcagaag ggtacgatat agtgactcct
     2161 gtagttgtta caaatacaca ggaatatctg gatgttctct tgctttcgac aaaagaggaa
     2221 gtcaactatg ctgatgattt attagcagtc ttataattag aggagggtat atgtcaattt
     2281 ttccagacaa tttcttatgg ggaggagcag tagctgcaaa ccaagtagaa ggtgcatata
     2341 atgagggagg aaaaggatta tccgttcaag atgttcttcc caatggtgga ctgggtgaag
     2401 ctacccaaga accaacggaa gataatttga aattacaagc tattgatttt taccatcgat
     2461 acaaggaaga tatagccctg ttttcagagt tgggatttaa ggtatttaga acatcaattg
     2521 cttggagtag aatatttccg aatggagatg aagaatttcc aaatgaagaa gggctacagt
     2581 tttatgatga actttttgat gaacttctaa agaatggtat agagcctctt gtaactcttt
     2641 ctcactatga aactcccctg tatctggcaa ggaaatatca tggctgggtg gatagaaaaa
     2701 tgattcaatt ttttgaaaaa tttgctcgta cagtttttga gcgatacaaa gataaggtta
     2761 agtactggtt gacatttaac gaggtcaatt ctgtcttaga actgcctttt acaagtggtg
     2821 gaattgactt gccgaatgaa gaactttcaa aacaagattt atatcaggcc attcaccatg
     2881 aacttgtagc ttctagtctt gttaccaaaa ttgctcatga aattattcct gactgtaagg
     2941 ttggctgtat ggtcttagct atgccgacct atccaatgac acctaatcct gaagatgttt
     3001 gggcggctca tgcctatgaa aatttgaact atttattttc ggatatacat gttagaggat
     3061 attatcctaa ttatgccaaa aggtacttta aagaaaatgg cataaagatt acttttatgg
     3121 aaggtgatga agagttatta aaaaataata cggttgattt tctctccttc agttattata
     3181 tgagtattac tcaggcagca gatccatcaa aatattcatc tggaagagga aatatcttgg
     3241 gtggtttggt caatccttat ttagaagctt ctgattgggg ttggcagatt gatccaattg
     3301 gtttaagatt gacactaaat cgttattatg atcgatacca gattccttta ttcattgttg
     3361 aaaatggttt gggagctaaa gatcagctag tgaaggatga ggctggtggt ttgactgttc
     3421 atgacgatta tcgaattcaa tacatgcaag aacacctttt acaagttgcg gaggcgatac
     3481 aggatggcgt agaagtcatg ggttatactt cttggggctg tattgattgt gtatctatgt
     3541 ctaccgcaca attatctaaa agatatggac taatttatgt tgatagaaat gatgatggaa
     3601 gcggcagtct aactagatat agaaagaaat cattttactg gtataaagaa gttattcaat
     3661 ccaatggcag tatcttgggc aatagctcaa aatagactct taaggtatta aaaagttttc
     3721 agaaaattta atcaaaagta ttgacaaagt gattttgata ccgtataatc atctgtaatc
     3781 agaagaagta gtaaagcaga agtcttcagg gagcctgtgg tgctgagaac aggtggcaga
     3841 gttttatgaa atgggctgat gtgcatttat gtctatttta tgacgtcgtt agtcggtctt
     3901 gccgtaccac cagtactgcc ttcgcccgac tgcctagtcc taaaatgccc taaattcaca
     3961 aggattgttt ggcataacat actttgcgta gactgttttg gctgagtacg aggcaggtcg
     4021 acgcagctta tactagcggt atagcaagga gagctaacga agtaatcagc caaaacagtc
     4081 acgtaaatga acttaaaaca atgatagttc ggtccgcaca ccttacagtg caactccttg
     4141 ttagaggaga tgagatatgg gagggttcta gacaattccc ataagtgagg tggcaccgtg
     4201 tcattgacgc cctcgcagga agttttttcc tgcgggggct ttttccttgt ctttgtagat
     4261 tgcttttata ttttgttacg attggcttgc ctaacttgag ttatgtctgc gcctccgttc
     4321 cttatctaaa agcaatctac tttgacaatt attaacaaaa cgaggtaatc atgatgaaac
     4381 gatggcgatg gtgggataat atattgatga cgtagaaatt gtatattgtg tattttacga
     4441 ggagaatgaa tatgttgaaa aataaaaatt tacttgctac aattgttgcc cttactgtta
     4501 tggtagttgc tgccctgttt ttgacgcaaa aagaacagaa caattcaacg aatagcacag
     4561 aaaaagtcaa aattggggtc ctccaatttg tgacgcatga ttcactagac gaaatttaca
     4621 agggaatcaa agcaggactt gaagaaggtg gttataccac aacggataat ttagaaattg
     4681 attttatgaa tgcagaaggt gatcagtcac aggttcaaac catgagtaaa aaattggtgg
     4741 acaatggcaa tgaattattg attggtatcg caacacctgc tgcacaaagt cttgccaatg
     4801 caaccacaga attacccatt atcatggggg ctgtaacaga tccagtcgga gccaacctgg
     4861 tgacggattt gaaaaatcct ggtggaaata ttacaggggt atctgaccaa acaccagtag
     4921 cagacgcagt ttctctcatt aaagaaatta caccagacgc taaaactatc ggtgtcctct
     4981 attcttcaaa tgaggacaat tcaaaaattc aagtagcgga atttaaggca gcagcagaag
     5041 aggctggcta tactgttctt gaatatgcag ttgcttctag taatgaaatt gcagcgacag
     5101 tagaggtcgc aagtagtaaa gcagatgttc tctttacacc agtagacaac acagttgcct
     5161 cagcattttc aactgtcgta tctgtggcaa ataaaaccaa aactccgatt ttcactagcg
     5221 tagaagacat ggtagaaggt ggaggtatcg catctgttac ccttagccaa tatgacttgg
     5281 gtgtggcaac tggtaagatg gctgcgaaaa ttcttgatgg tgccaatcca gcggatacac
     5341 ctgtacaaat ctttaacgag ggtaccgttg ttgtcaacca aaaagttgct aaagaattag
     5401 gaattacctt gtcagatgat gtgattagta aagcaagcaa ggttattgaa taagagataa
     5461 gagaaaagga aaaatatgat tgtatcaaca gtttcacagg ctcttttatg ggctgtacta
     5521 ggtttgggaa tattcgttac ctttcggatt ctgaattttc ctgatatgac gacagagggg
     5581 tctttcccgc ttggaggagc ggttgctgtt accttattga cgcaaggagt tcatcctatc
     5641 ttggctactc tagcagcagt tcttgttggc tgtttggcgg gctttgtcac aggcttgctt
     5701 tataccaagg ggaaaattcc cactctccta gctggtatct tggtcatgtc ttcctgtcac
     5761 tctgtcattc tatttatcat gggtagggca aatctaggct tactgaatac caaacgttta
     5821 caagatttcc ttcctttttc tgagcaagtc aatggtctct tgctaggtct aggtagccta
     5881 gcggtggtga ttggtgcctt gatgttcttc ctctatactc gctttggaca ggcctttatt
     5941 gcaaccgggg ataatccaga tatggctcgc agttttggta tcaatacaag tcgaatggag
     6001 ttgctaggtc ttgttctttc aaatgggatc atcgctcttg caggtgcctt gattgcacaa
     6061 caagaaggct atgcggatgt gtcgcgtggt attggtgtca ttgtggtcgg tatggctagt
     6121 ctgattatcg gtgaggtaat ttatggtaac cttactatgt tagaacgatt cttggccatt
     6181 gttgtcggag cgattttcta tcagttctta attttggtgg tcatttccct cggtatcaat
     6241 accagctacc tccgtatctt ctcggccatg attctggcaa cttgcctcat ggttcctgag
     6301 ttgaaaaata aacttctgaa aggagccagt ctgtcacgat gaaagcaatt gttgaattaa
     6361 aaaatgccac caaggtggtg acaaacggtt ttgatgaaga acgtgtgatt ttggataatg
     6421 tcaatctgac catttaccag ggtgatttta ttaccatttt gggtggaaat ggggctggga
     6481 agtcaaccct ctttaatgtc attgctggaa ccctaccctt gaccagtggt tcagtccata
     6541 ttcttggtga ggatgtgacc aattggcctg cagaaaaacg tgctaagtat ctggcacgtg
     6601 ttttccaaga tcccaaaatg ggtacagcgc cacggatgac ggtggcagaa aatctactga
     6661 tagctaagta ccgtggcgag gggcggggct tagtgccacg cagattggcc caacagacgg
     6721 aagaatttgc ccagcttttg ccgagagtgg gaaatggttt ggaaaaacat ttggacacgc
     6781 cagcagggct cttatcaggt ggtcaacgcc aggcattgag tttgttgatg gcgagtttga
     6841 aacagcctga acttttactc ttggatgagc atactgcagc cctcgaccct aaaaccagcc
     6901 aatccttgat gcaattaacg gacgagttga ttgagaccaa gggaatgacg gccctcatgg
     6961 tcactcacca gatggaagat gctctgcgtc acggtaatcg cttgattgtc atgaaaaatg
     7021 gtcagattat tcaggaccaa acggctgaag aaaaatccaa gatgaaactg gaagattatt
     7081 ataagttgtt tgattaagaa cctgtgtgga tagttcgcaa cttccaacca agtttggttt
     7141 tggctaaaac tgaatcttat atcaaacaga acacttgtga atacaggtct aaagtctgga
     7201 gtaatccagg cttttattat cctttgaatt tttagttaaa aagtagtata atatactaaa
     7261 tatgtcaaga ggtgctgtca tggataaatt ggtttttgaa agcttagctg acttgggaat
     7321 ggactacatc gttgtagagc atccaccagc tctgacaact gaggaagcag accgttacat
     7381 tgaagggtta gaaggagtac ggaccaagtc catgtttctg actaataaga aaaagactgc
     7441 attttattta ctgatcatgg atgaccagaa gcagctggat atggaccggt ttcgtgattt
     7501 ggtgggagcc aaccgcattc ggatggcctc gagtgatagc ttgatggaaa aaatgcactt
     7561 gcctgcgggt gcggtatctg tttttggctt gttacataat gttgataagg atattcaagt
     7621 cttttttgat aaagaaatcc tttctgaacc cattctaacc ttccacccca atgttaatac
     7681 gaaaacgatt tttgtaaaaa cagaggatgt tttgcgtttt gtagaagaga ttggcttttc
     7741 agcaaagatt gttgacttgg ggtaacaaag atgagaaaat gtttagctat ttgtagtctg
     7801 ttgtgttgtc tttttttggt aggatgtcgg acaggagcag cttatcaggc agagattgaa
     7861 ccgatggaac aatcagaatt gtatacggaa aaagagattg aggcggctat tcttgtagtt
     7921 caagattact ttgatcacca ttttaacagt tgtaaattgc tgaccatcgg ttacagtggt
     7981 gataatgaga aggaatttga tgagtgggcg gagcactatg gtgcagagga agctattatc
     8041 ctaacttcgt cttttaaggt agcagctgag ggggcagagc ccaccttaga gcctaatagt
     8101 acccatacgg attggaaatg gattttagtg cggaatgttg gtggtaagtg ggaacataag
     8161 ggacatggtt actaatgtat atgtagaact tcgttgattt tcagcggagt tttttgtaaa
     8221 ttttcttgaa aaaatgtata atagaagcga tagctattta ggtatttcta tttaaaagtt
     8281 gcgttgatgg ttgaatataa ttttgaacca aagtgtgtct tttgcaaata gagctccgac
     8341 atagcagact gtaattcgac ttggaactcc caaggaccgt tggtaagaaa aataaaatgg
     8401 acacctgtgg gatgtccaaa aggagtaaag atgagtacaa ttaaaattat ggctcttggg
     8461 ggtgtgcggg aaaatggaaa aaacctgtac attgccgaag tgaatgaaca tatttttgtt
     8521 ttggatgcag gggcaaagta tcctgagaat gaacagttag gtgtggatgt agtcgtccca
     8581 aatttcgatt atttggaaga aaataagcat cgtgtagcag gggtcttttt atctcacggg
     8641 catgcagatg ctatcggagc gcttccttat ctcttggaga aagtgaaagt gccagtattt
     8701 ggttcgcact tgactatcga attggctaag ctacttgtca aggggaataa tgctactaag
     8761 aaatttaatg atttccatgt tatcaatgct gaaactgaaa ttgatttcgg agattcagtg
     8821 gtatctttct ttcagacgac ccactctatt ccagagagtt taggaattgt cgttaagacg
     8881 gatgagggaa atattgtcta cacaggtgac tttaagtttg accaggcggc agataagttt
     8941 tatcgtacgg attttgggcg attggctgaa atcggtaacg aaggtgtgtt ggccctcttg
     9001 tcagactcag ctaatgcaga tagcaatgtt caagtagcga gcatgcatga ggctggggag
     9061 gaaattctca atacgattgc tgactgtgac gggcgtgtta ttgtggcagc agttgccagc
     9121 aatattgtac gtattcaaca gatttttgac gcggcagaag caactggtcg tcgtgtcgtg
     9181 ttgacaggac atgatgtgga aaacattgtc cgaacagcga ttcaattgaa aaaacttcgt
     9241 ttggtcagcg aacgcttgtt ggtaaaacca aaggatatga acaagtatga agaccatgaa
     9301 ttgattattt tagaaactgg tcgtatgggc gagccgttaa atggattgcg taaaatgtct
     9361 atcggtagac atcgctacgt tgaaatcaaa gaaggtgatc tagtttatat tgttaccact
     9421 ccaagtattt caaaagaagc ggttgttgct cgtgtggaga acttgattta caaggctggt
     9481 ggtaccgtaa aatctatcac aaagaatctt cgcgtctctg ggcatgggaa tgctcgtgat
     9541 ttgcagctca tgctcaacat catgaagcct aaatatcttt tcccagttca aggggagtat
     9601 cgtcaattgg atgctcacgc taaggctgcg cttgaaattg gtatgtatcc agaaaacatt
     9661 atcatcgtta agcgtgggga tgtcatggct tttgaagagg gggattttgt ccatagcgga
     9721 gcggttcctg ctggtgatgt catgattgac ggaaatgcca tgggcgatgt tggaaatatc
     9781 gtccttcgtg accgtaaaat cctatcagaa gacggtatct ttatcgtggc tatcactgtc
     9841 aaccgccgtg agaaaaaaat catttctaaa gccaaggtca atactcgtgg ttttgtctac
     9901 gtgaaaaaga gcaaggacat cttgcgtgag gcatcagaat tggtgaatac aacggtggaa
     9961 aattacttta caaaggatag ctttgactgg gcagagctta agagtgctgt tcgcgatgat
    10021 ttagcaaaat tcctctttga ccaaactaaa cgtcgcccag ctattttgcc ggtaattatg
    10081 gaagtcaagt aagctgattt atatacataa gaagagccga gggaaactca ggctctttac
    10141 cataaggaga agatttatgg caataatgaa aattgaatac cattcagaag ctctagatat
    10201 gtctagacag gtaacggttt tatatccaga tcgaaatcgt gtggaaaatc ccgatgacaa
    10261 cgatattcct gtcctatatc ttttgcacgg tatgggagga aatcaggatt cctggctgaa
    10321 tcgttctaca gtggaacggt tggttcgtta taccaatctt atcgtggtta tggtcaatac
    10381 agataaaggt tggtatacca ataccactta cggtatgaat tacttcgatg ccattgcagt
    10441 agagttgcct caaattctca aacgattttt cccaaatatg tctgacaagc gggagaagaa
    10501 ttttatcgct ggtttgtcta tgggtggtta tgggaccttc aagattgcca tgatgaccaa
    10561 tcgtttttca cacgcagctt cgctttcagg cgcattgtat tttgattttg ataatccagc
    10621 ggcagcagaa ttgggaagtc tttcgtattg gcaaggagta tttggtgaca tttctgataa
    10681 acagaatccg aataatttgt tagaaattgc taaacaatcc gataaaaaga ctaagtttta
    10741 tgcctggtgt ggtgaggaag attttctctt tgaagggcat caaaaggcag ttggggagtt
    10801 gaaagagctg ggatttgata tagaggcaag tttcggacct ggtaaacatg agtggtacta
    10861 ctggaatcag cagattgaga aggtattggc ttggttgcca attgatttca aattggaaga
    10921 aagactgtcc tagtttttaa gaaaagtttc agaaattatg gctatactaa aacctatttc
    10981 tagaaagagg tatagtctat gaaacttatt ggaaatattc tacggtggat ttggcggaca
    11041 ttttggggtc tgatttggtt atcaatgatg ttattggctt gtggtctagg tctattattc
    11101 tattttcaac aggaagcagc tcctcagatg ttacaaactc tagctcaaga agtgcaattg
    11161 atggtcaaag gacagtctga agtgacgatt gaggaaggtg ttaataccat taagcacctg
    11221 acgactgata cggtcaatac agctgatcat ggtcgatggg aaagcaatac tgcgactgtt
    11281 tacattgaaa cccagaatcc aacatttgta gctgcctatg aaacagccat tgcaaattgg
    11341 aacacgacag gggcatttac ttttgtgatg acaagtgatc caaatcaggc tgatattatt
    11401 gctactgaga tgaatgatgg aaatacccag gcagcaggtg aggccaattc aacgactaat
    11461 ttattgacga attattatag ctcggtaacg gtgcgtctga atagttttta tttattaaac
    11521 gaccaatatg gttatgatat ggatagaatt atccatacag ctgagcatga attgggccat
    11581 gctattggcc tagatcatga agatagtcag acctctgtta tggaatcggc aggttctaat
    11641 catggtatcc agcaggcaga tattgatgcg gtccttgcct tgtattcaga atagaaggga
    11701 aaatatgctt cgaacaatta caaacacaat aaaacgttat cctgagcaag ctctgctctt
    11761 tctctataat gctgggattt ttgcatggat gcagtctacc agtcattcga ttatggaaca
    11821 aatcggtata gatagcaact ggtttgataa aatccccgaa cctatcaagg cctggacagg
    11881 tgccagtcta gagagtatgc aaaccttact caattcttct gcttggggat ggctgattgt
    11941 atctatgata ttgatgttgg tcatccgctt tgttaagggc ttgattaagt ttgtcatcat
    12001 gctcatcatt attggtggtg gtctttatct gctctggcag aataaggagc ttgttcaatc
    12061 cttggtataa atagaggaat caaatcagcc ttgtgctgat ttttttggta taatattttc
    12121 atgattattc taagtggaaa caagattgaa cgctcttttg cgggcgaagt tttatttaac
    12181 aatatcaaca ttcaagtgga cgagcgggat cggattgccc ttgtcggaaa aaatggggca
    12241 ggcaagtcta ctctgcttaa aatccttgtc ggagaagaga ctgcgacaag cggagagatt
    12301 tcaaccaagc gtgatttgtc actgtcttat ctggcacagg acagccgttt tgagtcggca
    12361 aataccattt acgatgaaat gctccatgtt tttgacgacc tgcggacgac ggaaaaacgc
    12421 ctgcggtcta tggaagagca gatgggcagc ctatctggta gcgacctaga tcaactgatg
    12481 aaaacctacg atagtctgtc agaggaattt cgtctggcag gtggttttag ctatgaggcg
    12541 gatattcgtg ccattttgaa cggctttaag tttgaccaga ccatgtggga catgaaaatc
    12601 tctgaactct caggtggtca gaatactcga cttgcactgg caaaaatgct tcttgaaagc
    12661 ccagaactct tggtcctgga cgagccgacc aaccacctgg acatcgacac gattgcttgg
    12721 ttagaaaact acctagtgca ttacaaggga gccttgatta tcgtcagcca tgaccgctat
    12781 ttcttggaca aggtggcgac actgacactg gacttgacca agcattcctt ggatcgctat
    12841 gtgggcaatt attctcagtt tgtcgagctc aaagagcaga agttgcaaac agaattgcaa
    12901 aactatgaaa agcaacagaa ggaaattgcc aaactagaag actttgtcca gaaaaatatt
    12961 gtccgtgctt cgaccaccaa gcgcgctcag gctagacgca agcagttgga aaagatggag
    13021 cgactggaca agccaacaac tggtcagaag tctgctaaca tgaccttcca gtcggacaag
    13081 acttctggca atatcgtctt gacagtggaa aatgctgcgg tgggctacga tggagatatc
    13141 ctttctcagc ccatttctat tgaccaacgg aaactagatg ccattgccat tgtcggtccg
    13201 aatggaatcg gaaaaactac cctgctcaaa tcaatcatcg gagctttgcc atttatcaaa
    13261 ggggaagcca agctaggtgc caatgtggaa gtgggttact acgaccagac ccagtcagcc
    13321 ctaacacctt ccaatacagt cttggaggag ctctggtcag cctttccgac tacgcctgaa
    13381 gtggagatcc gcaaccgcct tggagccttc ctcttctcag gagatgatgt caaaaagtcc
    13441 gttgccatgc tgtcgggtgg ggaaaaggcc cgtctgctcc tcgctaagct atctatggaa
    13501 aacaacaact tcctcatcct cgacgagccg accaaccacc tggacatcga cagtaaggag
    13561 gtcctggaaa atgcccttat cgactttgac ggcaccctgc tctttgtcag ccacgaccgc
    13621 tatttcatca accgcgtcgc caccaaggtg ctcgaaatct cggaaacggg ctccacgctc
    13681 tacttgggcg actatgacta ttatttggag aaaaaggcag agctggaagt agaagcccag
    13741 cctgaccagc tagaaagtac tagccaatca gccggagcca tggactacca agcccaaaag
    13801 gaaaaccaaa aagagcaacg caaactagct cgccgcatcg agcaaatcga agcagaaatc
    13861 gagacaatcg aaaatcgcct gagcgagctc aaccaagcca tgttagaaac caacgacatt
    13921 ggccagttga ccgattacca gaaagaaatc gaccaactga ccagccaaca agaaagtctt
    13981 atggaagaat gggaagaatt atctgagcag atggggtaag aaataagatg atccaacaga
    14041 atacagaaac actcgaagat aacttacgat ttaataaatt atccaaactg attcagacaa
    14101 aattgtccca atattttgaa acctatcctt cagctgtcca gtgccaatta gtttgttttg
    14161 atagagatga aactcgtaga aaagtcctta gctatgacat ctattccgat tttgataagc
    14221 tcttctgggt agagtcaata gcaaagccta tttctagtta ttcctacgga catcaaattg
    14281 ataaaaaaga tatagacaaa ctagataggg agtttatctt ggatactttt cttggaatag
    14341 atgaagaaag aggatagatt ttcatgacca aacaccaacg tttccttaat agcgaccaat
    14401 tttttgcctg tccacattgt gggcaggctc ttggtcttga gctcaacagc ctccgctgtc
    14461 ccaaccgcca caccttcgac atcgccaagc agggctacgt caatctggca ccccaggtca
    14521 agcagtctgc taactaccac aagtctagct ttgaaaaccg ccaggccttt ttagaggcag
    14581 gctattacga tcatctctac gaggctttgg agaggaaaat agcagagctg ggcttgcggt
    14641 ctgtcttgga catcggctgt ggagagggct tttattcccg taagttagcc gagaaaatgg
    14701 acttggacat tcttgctttt gatatttcca aggattccat tctcttggca gccaaatctg
    14761 acaggaccaa gtcggtcaaa tggtttgtca gtgacttgac caagttacct attcaagata
    14821 agaccattga cggcatcttg gacatctttt ccccagccaa ttatcaggaa tttgccagag
    14881 tgctgaaagc tggtggggca attctaaaat tagtcccagg ccccaatcac ctcaaggagc
    14941 tccgccattt agccaaagac caactccgca aggaatccta tgataaccag gatattgtgg
    15001 accatttcaa gtcttacctg ggacaggtgg agcaagtgct agtcagtcga actctgccga
    15061 ttacagccgc ccatgctcag gtcttggcag acatgacacc cctctttttc caagtagacc
    15121 agtccaagtt ggatttgagc cagttgaccg agattacgat tgagggagtg ctcttgattg
    15181 gaactatata aaaaatgaaa ccgcctctgg atatcagaag cggttttgct ttatttctca
    15241 aactctgcct tgcttttcac atcgccgtat tcttcagcga cttctttgct gaggatgtcg
    15301 gcgtagctgg caagttggat gtcttgtccg tattttttgc ggagggcagt agtgaccagg
    15361 cggtaggcca ggttggcatt gcgggataag attggtccgt ggaagtagct accgaaggtg
    15421 tttttgtagt gcagaccttc gccgccgtct tccttgttat tgccattacc gtagacaacc
    15481 ttgcccagcg gtttttggtt gtcagctagg aaagtacgcc cttggtggtt ttcaaaaccg
    15541 tagtaggttt cgttgaactc atcattgtga attttgatgt caccgatgta gcggttattg
    15601 gtctggttga gggtgtagtg gcccatgatt cctagaccgt caatcttgcg accgccagct
    15661 tcaatataat attgaccgag caactggaag ccaccgcaga tagctagcat gacgccatcg
    15721 ttttcgataa agtctgctag gctgtctttt tttgtcggta aatccttagc aagaatggat
    15781 tgttcatagt cctgtccacc gccgaaaaag gcgatgtcgt agaaattttt gtcaaattca
    15841 tctgtcaggg aaacgatgtc cacctggaca cgagccccca gcttttcagc cacatactta
    15901 agcatgagga tattgccatt gtcaccgtag gtgttcatca aatcaccgta gaggtgggcg
    15961 atgtggagat cgtaagtgta atctttttca ggacttttta atgacgtata gaccattatt
    16021 tcatctcctt tccaacggct tggcgttgag ccagcaggtc acggaattcg agcatagctg
    16081 tataagtagc taaaatataa gcatgctggc tactcgattg ctcaatcaag gccatgatat
    16141 cttctagttt tggtgtttca gtaatctggt tctcaggata gccagtcaca cggaggcgac
    16201 gggcgatttc agacgagcgg acaccacctg caaagacttg tggaatgtcc atttgagtga
    16261 tttgttcaaa gtcagcgtcc caaatccagc tggtatcaat tccgtcagcg tagttggcat
    16321 tgagaaggac ggacaggcta aaatcgtatg gagccagttt catcatttca atagcctgag
    16381 tcgcaccgac cgggttttta atcaagacca gtgtacaatc tttatcaccc agcttgaagg
    16441 tttcttggcg accgaagact gccttggact tgtcaaaacc tgccttgatt ttttcaggag
    16501 ttacaccgaa aaatgctgct acgctgacag ctgctagggc attgtagatg ttgtagaggc
    16561 ctccgatgtt gattttgtaa tcctgaccgt caatggcaaa ggtagaagaa gtattggtga
    16621 tttctttcaa gtctgtcaac ttatagtcca gctctggtcg gcaaaagcca cagttgttac
    16681 agatatagct gcccaagtta gcgtaggtat tgagcttgta ctggataatc tggtggcaat
    16741 gaggacagag aacgccttcg gtattgtaat gggccagacg aggttcatgt tcttccgtcg
    16801 caaaaccgta gtattttact ggattgataa cgtttgtcga gttaaaaagt ggactatcac
    16861 cattggccaa aatagttgcc tgcggagctt ttgcagcccc gtccaaaatc atctggtagg
    16921 tggtataaat ctcaccgtag cggtccatct ggtcacggaa gatattggtg aaaacaatca
    16981 ggctcggctt gataaagtcg gttacacggg caagacttgc ttcgtcaatc tccaagacgg
    17041 caatcttctt gcccttttta tttttcttgg cagacaaaaa ggttgctgta attcctgtga
    17101 tcatattggc accgctggta ttagtcgtga tttctccaaa ggcttcttgg agaatcccaa
    17161 cggtcaaggc agttgttaaa gtcttaccat tggttccagt aataacaacg acttcataat
    17221 ctttagccaa actatctaaa atatgtttat cgaatttgag ggcaattttc ccaggcagag
    17281 tagtaccacg tcctagtttt ttcaaaatga actgggaact ttttcctgct agaataccca
    17341 aagctgtatt tactttcatg ggactatttt atcataaaga ctggggattt tctagaatgg
    17401 agtgtgttgg aacgtgatat attcctgcag taaatctgta atatttttaa caggaaggtc
    17461 actattttct aaagaaaagg ggaaaaaata tggtataatg agtacattac tatgtatgag
    17521 ttagaaagta tgagaatgat atgttaaacc taaaccagtt actggataca gggtattggt
    17581 cgagtttgat agccagtcct tggaccgcgc tgctgcacct aattgatatt agtatagttg
    17641 tttatttgat ttataatttt agtaaggcta ttgcaggtac taaaattatg acattgattc
    17701 gaggggtttt tctcttcatt attgtccaga ttatagcgag tttattcggt ctgcagacga
    17761 ttgcttggct gattaatcaa gtaatcacgt atggggttat tgcggcagta gttatttttg
    17821 caccagagtt gcgtgctatg cttgaaaaac tcggtaggac aactcagatt tttgggacaa
    17881 atacagtgag tgcagaagaa aagttaattg ttgcttttct caaatcggtt gcttatatgt
    17941 ctccgcgtaa aatcggagcg cttgtggcag tagaacaagc ccaaacattg caagaatata
    18001 gagcaacggg gattccactt aatgcggata tatcacagga attgttaata aatattttca
    18061 tcccgaatac accgttacat gatggggctg taattgtcaa ggaagacaag gtagctgttg
    18121 cttgtgctta tttgcccctg tcagagagcg caggaatttc aaaggaattt ggaacacgac
    18181 atcgtgcagc cattggctta tcggaggtat ctgatgcgtt tgtatttatt gtctctgaag
    18241 aaactggcag tatctctatt gcacgaaacg ggatattcaa acatgatttg actttggatg
    18301 agtttgaagc agagttgcga gcgactttta tttctgaaaa tgtagagaaa aagtccatct
    18361 ggaagcgatt aggaggtggc aaacatgaac gataagttta aagctgtagg tcacttagcc
    18421 ttatctattt ttctagcatt gttgctcttt ttctatgcaa caaccaccaa ctataaaaat
    18481 tcagaatcag ctgcacagaa cacggagtca gaaacctatg ttcacacact gagcaatgta
    18541 ccgattgaaa ttgaatatga tacaaacaag tattttattt ctggactttc ttcgacggtg
    18601 gcagttgaat taaggggctc taatcgtgtt ctcctacaga gagaatcaga tgagtcaact
    18661 cggacgtttc aagtgactgc ggatttgaga gagttgagtg atgggacaca gactgtcaag
    18721 ctacaattga ccaatttgcc ggctggagtg agtgcgacac tagcacctga tgcgattacc
    18781 gtcaagattg gtaaaaaagt tagcaaggca ttcccagtag caggtcgagt atatagtaat
    18841 caattggcag aaggttacag tctgtctaag gtttcagtca acgtagatac cgttaaagtg
    18901 acaacggatg aagaaacaat ggctaagatt gatcgggtcg aagctgttgc gatggatgtg
    18961 agcaatctgt ctgagaatta tagtggtaca gctaaattac aggctgtaga tagcgacggc
    19021 aatgtcttgc cagttgtact gtctcagacg gaagcaaata tgcaaattat acttacaaaa
    19081 acaaaataag aggttatgaa aatgggtaaa tattttggga cagatggtgt ccgtggagaa
    19141 gcaaacgtag aattaacgcc agaattagca ttcaaattgg gtcgttttgg tggttatgtc
    19201 cttagccagc atgagacgga tgttcctcgt gtctttgtgg cgcgtgacac acgtatctca
    19261 ggtcaaatgt tagaggctgc cttgattgca ggacttctat cagtagggat tcatgtgtat
    19321 aaattgggag ttttggcgac accgggtgtt gcccacctag tcaaaactga aaaagcaagt
    19381 gcaggtgtca tgatttctgc aagtcataac ccagcacaag ataacggtat caaattcttt
    19441 gctggtgatg gtttcaaatt ggatgatgca ttggaagcgg aaattgaggc tcttcttgac
    19501 gctgaggaag acacattgcc acgcccatct gctcaaggtt taggagatgt ggtagaatat
    19561 ccagaaggtc tacgtaagta ccagcaattc ttggtgtcaa ctggtacaga tttggacggt
    19621 atgaaagttg ccctcgatac agccaatggt gccgcagcaa catctgcacg tcaaattttt
    19681 gtagatttgg gagcagattt gacagtaatg gctgaaaagc cagacggttt aaatatcaat
    19741 gagggtgttg gttcaactca tcctgaaaaa ttgcaagaac tggtcaagga aacaggaagt
    19801 caaattggac ttgcctttga tggtgacagt gaccgcttga ttgccgtcga tgaaaatggt
    19861 gatttagtag acggtgaccg tatcatgtac atcgttggta aataccttgc tgatcgaggt
    19921 ctattggcca aaaatacaat tgtgaccaca gttatgtcta accttggttt ccataaggct
    19981 ttggatcgtg aaggtattga aaaagctgtg accgcagttg gtgaccgcta tgtggttgaa
    20041 gaaatgcgca aggaaggcta caatgtcggt ggtgagcagt caggacacgt gattctgatg
    20101 gattataata ccacaggcga cggccagttg accgctgtgc aattgaccaa aatcatgaaa
    20161 gaaactggta agaagttgtc agaattggca gcagaagtga ccatttaccc acaaaaattg
    20221 gtcaatatcc gtgtggaaaa cagcatgaaa gacaaggcaa tggaagtgcc tgctattgca
    20281 gctatcattg aaaaaatgga agcagaaatg gctggcaacg gtcgtatctt ggtacgccca
    20341 agtggtactg agcccctctt gcgtgtcatg gcagaagcgc caacggatgc tgaagtcgat
    20401 tactacgtgg acaccattgc cgatgtggtc cgtgcggaaa ttggaattta agcagaaatc
    20461 aatcatttgt taagatgatt tcattgtctt gaactaccag attgattagg tggctagtag
    20521 ttccactcaa gactgcgaga ataaaagtaa aaactggagt ttggttactc cagttctttg
    20581 atgttgtgag gaaattatga gtattttaga agtaaagaat ttaagtcatg gttttggtga
    20641 ccgcgcaatt tttgagaatg tctccttccg tctcttgaaa ggggagcata tcggtcttgt
    20701 cggtgccaat ggagaaggga aatcgacctt catgtccatc gtgacaggtc aattgcagcc
    20761 agatgaaggt aaggttgaat ggtcacgtta tgtaacggca ggttatttag accagcatgc
    20821 taaattagaa aagggtcaat cagtccgtga tgtcttgcgg acagcattcg atgaactctt
    20881 caagacagaa gcccgtatca atgacattta catgtctatg gcagaagagg gtgccgatat
    20941 ggatgccctc atggaagagg ttggtgaact gcaggatcgt ctggaaagcc gtgatttcta
    21001 tacgcttgat gccaagattg acgaagtagc gcgtgcgctt ggtgtcatgg actatggcat
    21061 ggacaaggat gtgacggagt tatcaggggg acaacgaact aaagttcttc ttgcaaaatt
    21121 gctcctggaa aagcctgaca tcttgcttct ggatgagccg accaactact tggatgctga
    21181 gcatattgac tggctcaaac gctacttgca gaactatgaa aatgcctttg tcttgatttc
    21241 gcatgacatt cctttcttga atgatgtgat aaacattgtc taccatgtgg aaaatcagct
    21301 cttgactcgc tatacaggtg attactacca attccaagaa gtccatgcta tgaagcgggc
    21361 tcagttggag gcagcttatg agcgccagca gaaggaaatt gcagacttgc aggacttcgt
    21421 taaccgtaat aaggcccgtg ttgcgactcg aaacatggcc atgtctcgtc agaagaaatt
    21481 ggacaagatg gagattattg agcttcaagc agagaaacca aagccatcat ttgatttcaa
    21541 aatggctcga actccaagtc gctttatctt ccaaacgact gatttggaaa ttggttatga
    21601 ccgtgtattg acacgtaaac cgctcaatct gacctttgaa cgcaaccaga agattgccat
    21661 tgtcggtgcc aacggtatcg gtaaatccac tcttcttaaa agtttgctgg ggattattcc
    21721 accgctgggc ggttctgtag agcgtgggga gtatttggaa ctaggctatt tcgagcaaga
    21781 agtagctggt ggcaatcgtc agactccgct tgaagccgtt tgggatgcct ttccagccct
    21841 taaccaggcg gaagttcggg cagccttggc tcgttgcggt ctgacttcca agcatatcga
    21901 aagccaaatt caagttcttt caggtggtga gcaagccaaa gttcgcttct gtttgcttat
    21961 gaaccgtgaa aacaatgtct tggttctcga cgagccgacc aaccacttgg atgtggatgc
    22021 caaggatgaa ctcaaacgtg cccttcaagc ctacaagggc tctatcctca tggtctgcca
    22081 cgagcctgat ttctatgaag gctgggtgga cgatgtatgg gattttaatg aattgactta
    22141 gattgaaagc tatcgaacga tagctttttc tttgtgctta tactataata gaatgagcat
    22201 agtaaaggag gtaaaaaatg aaaagaacag aacgaggcca gatttgctag gcctctctag
    22261 aggaagtaaa acggtctggg gatagaccgt tttagctcaa caactagaaa taaagacttg
    22321 ttgacgaact ctttttgacc agtcgagttc tttcccactc gcgtttcttt gacggttgga
    22381 aagacagtac cgcttgactg gtttgaaaaa gcccaaggta agaaattgct gggtctagca
    22441 tgcggtggtg gtcaacaggg accaattttt gcggctcatg gctatgagac aactattttg
    22501 gacttctcca aggaacagtt ggacaaggat aggttagttg ctgagcggga aaatctggat
    22561 ttgcaaacgg tccaggcaga tatgacacaa gcctttcttt ttgaagacga gagttttgat
    22621 atcatctttt gtcctgtttc aaatgtttat gtcgaagatt tgaccaatat gtggcaggag
    22681 tcttatcggg tcttgaaaaa aggaggcttg ctcatggttg gctacatgaa cccttggatt
    22741 tatgtatttg acagtgacga tgtttgggat tatccagaca agaccttggt tccaaagtat
    22801 agcctacctt tcaacgcacg acaattagaa gaagcaggac aaatcacgat tgatccagaa
    22861 tatggctatg aatttagcca caccttagaa gaacagattg ctgggcaatt acgagctggt
    22921 tttgccatga ttgattttta tgaatccaaa gacagtcgca atcgcttgac tcaatatgcc
    22981 tcagattacc tagcgaattt atcgataaag tggtagaaac aagccaccga aagatggtct
    23041 tttcaactca gtaggtaaag ttgaaatgat tgtgcaagat ttttcaggct tccgctgtat
    23101 aatatctcat tttttatgct attgacctga ctggattttt ctgctatgat tgaaaaatgt
    23161 ttgtttggag gtaaatgagt gaataaaata atatctgtat ggaaaaaaat gaatttaatc
    23221 cgaaaaatcg gaattggtgt tgttttggga gtgttactag gcttaattgc tccaaaaata
    23281 acggtcattg ctctctttgg tagtctattt gtaggagcat tgaaagctat agcccctctt
    23341 ttggttctga cactagttgc acatgctcta tcacaagctc ctgctggtca aaaatcgaat
    23401 atgaggacag ttatctgctt gtatctattt ggcacttttg cggcggcctt cattgctgta
    23461 ggagcaagct atttattccc tattaaactg gttctttcta ctacaacgac tacggacatt
    23521 attcctccac aagggattgc tgaagtattc caagacctct tgttgaaggt cgttgacaat
    23581 ccaatcaatg ctcttgcaac ggctaattat attggtgtgt tgacatgggc ggcagtgttt
    23641 ggcttggcct ttcgtcatgc aagcaagaca accaaagatt tattgcaatc tgcggcagaa
    23701 gtgacttcta aagttgtagg ctggattatt ggtctggcac catttggtat catgggcttg
    23761 gttttcgata ccattgcaaa taatggtctg acagctttaa aagactatgg tttactgctc
    23821 ttactcttgg ttggaagcat gatttttgtc gcgcttgttg tcaatccctt gattgctttt
    23881 cttgtaatga agaaaaatcc ttacccgctg gtatttgaat gtattcgggt tagtggggtt
    23941 acggcatttt ttacaagaag ttcagcagct aatatccctg tcaatatgca gctctgcaaa
    24001 cgtttaggag tggatcccga tacatactca gtatccattc cgctcggtgc cacaattaat
    24061 atggctggcg cggctattac gattaatatt ttaacaatgg cagctgttca tacacttggc
    24121 atcagtgttg atttcagctc agctcttctt ttatccgttg ttgcatcact atcagcagca
    24181 ggagcatctg gggtagcagg tggctcgctt ctcctcatcc cagtagcatg tagtcttttt
    24241 ggtattccaa atgagttagc catgcaagta gttggagtcg gatttgtggt cggagtgatt
    24301 caggactctt gtgaaacagc cctcaactca tcaaccgatg ttttgtttac agttgtagca
    24361 gaacgatctg cttggaaaaa atagaaagaa gttgggagac caactttttt tcttcgcttt
    24421 acttctgaaa tctctttcat tttgtgataa aatagactta ctttgatata gtctttgggt
    24481 ttacttttag aactaggcaa cgagccgcag gcataactgg agttaggcaa ggtgagttca
    24541 aacactccag tggagtgttt gaagttggaa ataaggaaac gatgtttcct cgctcgtcga
    24601 agtaataaaa gataaaccaa atgacgacac tgaaaaggag aacatatgac agtagatttt
    24661 agagcagagt ttgacaaacg caaagatgag tttctagcgg acctctttga cctcttacgc
    24721 atcaattctg agcgtgacga cagccaggca gatgcccagc acccatttgg gccaggacca
    24781 gtgcgtgcct tggacaagtt tctagaaatt gctcagcgtg atggctatcc gaccaagaac
    24841 gttgacaact acgcaggtca ctttgagttt ggtgaaggcg acgaagtcct tggtattttc
    24901 ggtcacttgg atgtggtgcc agcaggaagc ggatgggata ccgaccccta cgagccgcaa
    24961 atcatcgacg gtaggctctt tgcccgcggc tcctctgacg acaaggggcc aaccatggct
    25021 tgttactacg gcttgaaaat catcaaggag ttgggccttc cgacctctaa aaaagtccgt
    25081 ttcatcgtcg gtaccgatga agagtcaggc tgggcggaca tggattacta ctttgagaac
    25141 gttggtctgc cattaccaga tttcggattc tctccagacg ctgagttccc gattatcaac
    25201 ggcgaaaagg gaaatatcac agcttatctt cattttgcgg gggaaaatag cggagctgct
    25261 aaactgcatt ccttcacagg cggcctgcgt gagaacatgg tgcctgaatc tgcgacagct
    25321 attatctctg gcgacctagc agacctggac agcaagttgg cagatttcac agcagcctat
    25381 ggcctaaaag ctgacgcgga aacccttgaa aacggtcaaa ttcaagtcac cgtcatcgga
    25441 aaatcagccc acggttcaac cccagaagaa ggtgtcaacg gagcaaccta tttggcaaaa
    25501 ttccttagcc aatttgcctt tgatggagca gccaaagcct atcttgactt agcaggacaa
    25561 gtccttctgg aagaccacga tgcccaaaaa ctcggcgtag ccatctacga tgagcaaatg
    25621 ggagctcttt ctatgaatgc aggtgtcttt aagtttgatg aaacctcatc tgacaacaca
    25681 attgccctca acttccgtta tccaaaaaat accaaccctg aaaccatcaa ggctggtttg
    25741 gaaaaactgg gcgtggaagc tgttagcctg tctgagcatg gccatacccc acattactgc
    25801 ccaatcgatg acccaatggt cgcaaccctc ttgtctgttt atgaaaaaca cacaggcttg
    25861 aaaggtcacg aacaagtaat cggtggcgga acatttggac gcctgctcaa acgtggcgtt
    25921 gcctacggag ccatgttccc aggcgatgtc aacaccatgc accaagccaa cgaatttatc
    25981 gaagtcgagc aactctaccg cgccgctgcc atttacgcag aagctatcta cgaactcatt
    26041 aagtaataca tgaagacggg tacgcccgtc ttttttagga ggaagaatga aactgaaaga
    26101 actcaccgtt gcagatttgg aaatggtcaa acagctcttc ttgtctgtct ttagccaaga
    26161 accttggaat gatgattggt cagatgagga acaattagat tgctacatag gcgatttact
    26221 gggacaccca agagcactct gttttggcct gtttgacccg gaaaaagtga tagggctgtc
    26281 acttggttac attcgctatt ggtgtgaggg aacagagtat cgaattgaag agttctgcat
    26341 ttcccgtgat taccaagggc aaggacatgg tgctagttta ttagctgata ttgagaagca
    26401 gttagctgat agaaaaattg ttcatattct tctgcaaacc gaacgcaacc tttcagccta
    26461 ttctttctac aaaaaatgcg gtttccgagc cttagaagaa gatgtgacca tggtcaagaa
    26521 agtaggacat catggaactt gctgatattc gtcaagccat cattgaggat gttgctaacc
    26581 gagactttac gaacaaggga atagctcccc ttttccaagc tccttcgacc gcaaaaattc
    26641 tcattattgg acaggcacct ggcttgaaaa cccaggaaaa agggcgttta tttgacgacg
    26701 ccagtggtaa caaccttcgt caatggttgg gcgtggatcg acagactttt tatgaatcgg
    26761 gtcattttgc tattcttcct atggatttct attatccagg aaaagggaag tctggggatt
    26821 tgccgcctcg caaggatttt gcgcccaaat ggcacccact cattatgaag caactccccc
    26881 agattgagct gaccatattg gtaggcaatt atgctcaaac ttattatttg gaaaaatccc
    26941 aaaggaacct gacggagaga gtgaaacatg cagatgatta ccttcccaag tattttcctt
    27001 tgccccatcc atcacctaga aacaatatct ggcaagctaa acatccttgg tttgttgaac
    27061 agactctacc gatactgaaa aacaacataa atatattgtt gggaaaatga taatatgata
    27121 taattgtata tatataccta ataaattttt cttatctttg ttcatcgaag gaggtgttct
    27181 gatgagaaaa tggaaaaaaa ttatcatcgg ctgtttcgct attctaacgg tttgcttgct
    27241 atttgcaccc acactagcta gagcaaatca gatcccagac cgacccacag gtacaacggt
    27301 agtggatgag acgcagttac tctcgagtga gaccattgca gaaattgatc aactcaatcg
    27361 aagttgggca agcaccgagc aagctttaca ggttggtgtt tatgtaacgg aaagtctgtc
    27421 tagtgatatt gaaagtttgg ctaatgaaac ctttcgtcac tggcaggtcg gttttgcagg
    27481 aacggacaac ggagttcttt tagtggttgc catagctgac agagcatttc ggatagaaac
    27541 ctcagacaat gctgccacag tcatcacgga tgtagaagcc aaggatattc tagagaatgc
    27601 aagggaattc tttcgacagg aagactacag tggaggaatt tcctatatcg tcaactccat
    27661 tggggatagg ttttatggta caagcattgg taaaaatcag ttagctgcta tagaagaagg
    27721 tacaagtgag aaggatgacg gcttctggct gtttctcata gttattctga tcgctatcct
    27781 atttggtatt attgacaaat ctagtcgtgg tggtggaggt ccaggcaatc tactctggat
    27841 gttggttgat gatcatcatc actatcacaa tcatcactca tctaattcct catcgtctag
    27901 ttttggcgga ggtagttggt caggtggtgg aggcggcggt ggcggagcct cttctggatg
    27961 gtaaggcatc gtataaggct tctgatagtt gtttgctttt ttacgaatgt ttaatcgtag
    28021 ttagaaagga aatcttatga aaaagaaatg gttagccata cttctcccga ttctcgctct
    28081 gctgttttta ggaatgggag cagttgggca atacaatggc ttagttgaca gttatgctga
    28141 ggttgaaaat gcgcaagcca atgtagacac acagttgcaa cgtcgctatg acttgatacc
    28201 aaatgtagtc gcagctgtca aaggcgccat ggaacacgag gaagaaatat ttactgctat
    28261 tgcagaagcg cgtgctaaaa tcggttccag ccaagtagga accagcgact acaatcaggc
    28321 acaaagtcag ctagattcgg cagtgtctag attgttggta gtggctgaga attatccgca
    28381 attaacagcc aaccaacagg tatctgccct gataacagag cttgaaggta cggaaaatcg
    28441 gatactggta gcccgtaaag actataattc ggtagcaact acctacaata aaaaaatcaa
    28501 gcggttcccg acctccatct atgctaactt atttggctat gaaaaagtcg agcttttcca
    28561 ggcaacaaat gatgcggcga cgactgtacc aagtgttgat ttgagtgata aggaataaag
    28621 gagataaata tggactttgg atttgatata ttggcaatta tgataccaat tattgttgtt
    28681 gtactatttg ttttattgtt tgtatttggt tatgtgtctg ctaagcctaa cgaagcgatt
    28741 gtcattactg gcttgggcaa acctcggact ttaattggtc gttctggttt tatgattccc
    28801 tttatcgaaa aacgctccta tatctctatt gagcagtttt caacagatgt gcagaccaca
    28861 gattttgtac cgactcttga ttttattaac gtaaaagctg acgcggtcgt aaaagtaaag
    28921 gttgggatat ccgatgaatt gctcaatgcg gcagctcaaa acttccttaa ctggaaaact
    28981 gcagatattt ctgcctctat tcaagatgtc ttggaaggaa atctgcgtga aatcattggc
    29041 cagatggaac tgcgtgacat ggttaataac cgccaggctt ttgcagaaaa agtccaatcc
    29101 aacgcagctc ctgacttggc taaaatgggg ctggaaatca ttgcctttac ggttcaatcc
    29161 ttcacagatg acaacgatgt cattaaaaac ctgggtattg acaatattgt gaccatccag
    29221 aaggatgccg caaatgctcg tgccaaagca gagcgggaac aagcagaagt ccgtgcccgt
    29281 gaggataagg cggctaatga tgcgcgtgtt gctgcagact tagaaatcgc caagaaacag
    29341 aatgaactag ctattgagca agctaatcta aaacgtcagt ccgatgtcca actggcacag
    29401 gcaaacgcag cctatggcat tgaggagcaa gctcaacgga aagaaatcga gcgtgctact
    29461 gctgaagcca atattgtcaa gcaacaaaaa gaagcagaag taaaagcaga ggaagttaag
    29521 gtccgtgaac aagagctctc agctactatc cgcaaacagg cagaggcaga aaaatacgct
    29581 cgtcaacaag ctgcagaagc agatttgatt gaacgtcaac gcaaggcaga agcagaactc
    29641 tacgaaacgc agcgtgaagc ggaagctcaa aaagcgcgtg ccgaagctgc taagtatgct
    29701 gcggagcaag aagcggcagg tattgaggcc aaaggacgtg ccgaagcaga agccatccgt
    29761 ttgaaactcg aagcagaggc agaaggtctt agcaagaagg cggatgccat ggctaagttt
    29821 aatgatgcag cggtgacaga aatggtggtt aatgttcttc cagaaatcgc caagaatatt
    29881 gctgcaccac ttggaaatgt tgataagatt accatgtacg gcgaagggaa tgcctcaaaa
    29941 atggtgggag acctgatgac gaccatggac aagacaacag aaggtctagg actcaatgtc
    30001 cgtgatttaa tcagcgcaac cctaactggt cgtgccatgt caacaggttt actgactgcc
    30061 caagaaacaa aggaagcgga aggggacaaa taaaaataat aagcagttgg aagagatggg
    30121 tgactgagta ggtttacacc atcatttcca tcgcatgatt gtttcagaaa tccctacgaa
    30181 gatgaaaatg tttagaaaca gtaaaaaaag taagttgttt atacaaaaaa taaatgaact
    30241 attatctgat tcagaattaa agttgagcaa agcattgaaa ttccagttgt tggaagcaat
    30301 ggagttgtgt gaaaaaggta gtaagattag ctatctttca tataaaattt atccatgggt
    30361 attggaagaa ctagctttga ataggattca atcggataaa ttaaagatgt ttaagcgcta
    30421 tttagagcaa gaaaggtgga aatattattt tggttctgct ttgggaatgg cgtttacttc
    30481 gattagataa tggttccgag gatttgtaag tacattgatt gtgctgtgca acttcgatag
    30541 aatatgcgaa agtcccaaga aagagtaatc ttgggacttt tacaatcttt attgagattc
    30601 gtttttgttt atcagtcatt tacccacact ccgctgacat aattctcaga tagaatggtt
    30661 tgacttaaaa ctttttttgc ttgccagtca tagatgagtg ccttaccagc cagaatcaat
    30721 aataatctgt taccgtctag ttgacggaca tggtcaatat aataaggacg aggattaagc
    30781 tcgtctagac gatggtagct ctcttgacct gtttgggtat tgtaaatggt aaaagagatt
    30841 gggctaaaca tgtcattttg atgttcgatg aggagaagct tctggtcttt gagagcatgt
    30901 agtgtgcttg gtgaggggtg ttctaaagtt actgacgaaa atgtttttgt ttttgtatca
    30961 aaggttagga gttcggatga ctgttcaatg tcagtatact cacttgtttt ttccctaaat
    31021 ttgggagata gatggttcca ttgacccaag gcccctctcc tgtggcaatg gtattgtcgt
    31081 acttgaatac ctcttgtttt gaaaaatctt tcttatcaaa tacaatcaag acattttctt
    31141 cgggtccttc ttgggtaaaa tctttcacaa aaccagttaa atagactgac gtttggtcca
    31201 ccatgactgg agcatttggg atgaagtcat cgtttaaaac gtgggtttga atggtatcaa
    31261 aattcctgtc aaattgcgct agtatgtgat agggccccgc tgtataaaag tcttgtccat
    31321 ctgtaccact gatatagcta ctatactgac ttggctttcg caagacagtg ccagtattga
    31381 aatcgatgga tatcagattg tcgttaatga atttttgctg atcgttggca aagactagat
    31441 accgactgtc tatgacataa ttttcaccag aaccaaaata cccttctgaa ttcatttttt
    31501 gggtatgaag cagctctggt tcttgaccat ttagctgaaa ggcttcgacc ttaccgggat
    31561 aaatcaagta aaaatcagct tccgacgtta atgtgattgt attgtgctgg taggcatata
    31621 gtttccagcc gataaatgga aagacaagac aggatagaag gacaatacaa ataagaagtt
    31681 ttttcatggg aatgccgcct ttcactgttt agtatatcta aatttgtcat cttagacaag
    31741 aaattatgtg ttaatataca aataaagtca tgcaagtttt cttacatgac ttttgttctt
    31801 attttccaag acagaattgg ctaaagagtt gggtaatgag ttcatctgga gcagcatcac
    31861 ctgttatttc tcccaaaatt tgccaacagc gtgtcaggtc aacctgtaac aagtctactg
    31921 gcatgcccat ttccagccca tcgttgacag cttgtaggct ttggactgcc tgttcaatga
    31981 gggaaatgtg gcgagaattg gagaggtagg tggcatcttg ctcgaccaga cctgcatttt
    32041 caaagaagag ttgattgatt ttttcttcga tttggtcgat attctggttt ttcaagacgg
    32101 aaatgcggat gacatcctca ggcagttggt ctgcttcaat ctgttcttcc aggtcggtct
    32161 tgttgagtag gacgatacgg ttggccaggt ctgaaatggc taggaggttg cgatcttgct
    32221 cggtcagggg ttcagatgcg ttgaggacta gcaggatgag gtcggcctcc tcaagggctt
    32281 ttttagagcg ctccacaccg attttttcaa cgatgtcgtc tgtttcacgg atacctgctg
    32341 tatcaatgag cttgaggggg acgcctttga tgttgacgta ttcttcgata acgtcgcggg
    32401 ttgttcctgc gatgtctgta acgatggctt tttcctcgcg gaggaggtta ttgagcaggc
    32461 tggattttcc cacatttgga cgaccgataa tagcggttgc gatgccttca cgcaggattt
    32521 ttccacggcg ggcagtacgg aggagatttt ctagaagagc ttggaactgg agagttttct
    32581 cgcggaccag ctctgtcgtc gcctcctcga cgtcgtcata ctcagggtag tcgatgttga
    32641 cctcgacctg tgccagcgtg ttgagaatct cctggcgggt gtcgttaatg agctgggaga
    32701 gggagccgtc aagctggcgg acggcattgt gcatggcctt gtcggtcttg gcacggataa
    32761 catccatgac ggcctcggcc tgtgtcaaat ccacacgacc gttgagaaag gctcgcttgg
    32821 taaactcgcc cggctcagcc attcgagcac cttggcgaat caggagctgg agaatttcgt
    32881 tggtaacggc gatgcctccg tgggtattaa tctcaatgac atcttcgcgc gtgaaggtct
    32941 ttggggaacg catgacaccc agtatgacct cgtcaagcac ttgccctgtc gcagggtcaa
    33001 taatgtggcc gtagttgagg ctgtggctag gaaccgttgc taagtccttt cccttgaaaa
    33061 ccttgctggc aatggcaaaa gcatccgtcc cagacaggcg gacaatcccg atagcgccct
    33121 caccgagagg ggtggaaatg gctgttattg tgtcgaattc tttggtgatc ataataatgt
    33181 gctttctaac tagaatctgt atttacaagt aaaatacttg gatataagag atagtttgtt
    33241 gctaatcaag gcagtttttt gggggatggg ctcaagccca gtgccacttc tcagagttcg
    33301 tgtcaacatc tcagcgcagt ggttgattgg ctttaacagt ccagtggact gttaaaggtt
    33361 ggagatagga tttgtgaagc aaatctctgc aattcgtgtt tcacactcca aatccgacct
    33421 ctacgactgt tgcgaacaag ttcgcttcat ttccaacctc caacagtcac tactctgact
    33481 gttggagctg tgcgggggtg ggagtaaaat agtccggtgg agtgaaacag tttagggaac
    33541 tgtttcagct ggtcacctta aaactagaaa gtgaccatag aagctgaacc tactgtattg
    33601 gaagtgaggg aaactcgttt tgagatcaat cgagttcttt ctcactcccc aaaaactaac
    33661 gatgaatagc tgataaacta gctttagatg gaagtgctga actgtgaata caggttctta
    33721 aatcttactt ataagtttaa catggaatga agaaaaaagc acattttaga gatgctgata
    33781 caaagatatg tattgaaaac gttttgaaaa aggtgtaaaa tagatgtgga ataataaaga
    33841 gaaagtaggt tttgtatgtc aaacggtaaa attttaggcc ttgatattgg tattgcttcc
    33901 gtcggagtag gcgtcataga tgcacaaacg ggagaaatca ttcatgcaag ctctcgtatt
    33961 tttccctctg caaatgctgc taataatgca gaacgccgaa cttttcgtgg aagtcgtcga
    34021 tt
//
LOCUS       DAZQCJ010000010        33177 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000010 DAZQCJ010000000
VERSION     DAZQCJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33177
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.010"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            136..2082
                     /locus_tag="ACHU66_000458"
     CDS             136..2082
                     /locus_tag="ACHU66_000458"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFR3388768.1"
                     /translation="MDYKKVSAEVIEAVGKDNLVAAAHCATRLRLVLKDESKVNQKAL
                     DDNADVKGTFSTNGQYQVIIGPGDVNFVYAELIKATGLKEVSTDDLKQIAAQDKKFNP
                     LMDLIKLLSDIFVPIIPALVAGGLLMALRNFLTSPDLFGPKSIEEMYPAIQGISAMIQ
                     LMSAAPFMFLPVLVGISAAKRFGANQFLGAAIGMIMTTPDLGGKEAFWDIFGFQVAQT
                     NYAYQVIPVLAAVWLLATLEKFFHKKLPSAVDFTFTPLLSVMITGFLTFTVIGPVMLT
                     VSDAITNAIVWLYDTTGALGMSIFGGTYSLIVMTGLHQSFPAIETQLLSAYNNNGTGY
                     GDYIFVVASMANVAQGAATLAIYFLTKNAKTKGLSGSAAVSAFLGITEPALFGVNLKY
                     RFPFFCALAGSAIGALFAGLTHVIAVSLGAAGFIGFLSIKASSIPMYVVAEVIAFAAA
                     FAFTYVYGKTKASAVFADEAATAAAAAVTASVVETSEEVEETAEIFTAQETLVSPLSG
                     DVVALENVNDPVFSSGAMGKGLAVKPSEGVVYAPADAEVTIAFETGHAYGLKTASGAE
                     LLIHIGIDTVSMNGNGFEKLVAAGDKVKAGTPIAKFDATKIAEAGLDDTTMIIVTNTA
                     DFAEVSPLAEGTIAHGVEFLKVAK"
     gene            2210..3094
                     /gene="scrK"
                     /locus_tag="ACHU66_000459"
     CDS             2210..3094
                     /gene="scrK"
                     /locus_tag="ACHU66_000459"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFR3388769.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFQVVEKTQFPTTTPYETIERT
                     VEFFKRYEDRLAGIAIGSFGPIDIDQNSQTYGYITSTPKPHWSNIDLLGLIAKEFNIP
                     FYFTTDVNSSAFGETLVRKGVKSLVYYTIGTGIGAGAIQNGEFIGGLGHTEAGHTYVA
                     LHPYDVKHEFKGMCPFHNGCLEGLAAGPSLEGRTGIRGELIELNSDVWDVQAYYIAQA
                     AVQATLLYRPQVIVFGGGVMAQEHMLNRVREKFVGLMNDYLPTPDVKEYIVTPAVAEN
                     GSATLGNFALAKQVSRNS"
     gene            3708..6461
                     /locus_tag="ACHU66_000460"
     CDS             3708..6461
                     /locus_tag="ACHU66_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFR3388770.1"
                     /translation="MIPIQQHGKRWLILTLTLSTSVLALASIGASQPVVFADENSHLQ
                     SQKNINKIEVLDWKTFSKKLSEYSSDQRQFHVLKLGFENRLGTLSTWDELEEFGKNNN
                     FLVINGKVTQNIHDFPHILVMNKGDVIAHNEEDYHNQMRELRFSGNGDLHNSMEPKRI
                     HALFKIELDSNKRQLLNAAGLGTAENSLKNINGMTIYSHGLTVDNKYYEDYSKYTHNS
                     VKNINVTKERFIANDDLIHKLIESSEAMKQSSERDKVKAFVQYVANHTTYDWEAANKA
                     VQNYADINYYLGSDLFAVTERQKAMCVGFSTTAARAFNMLGLPAYVVVGKNAEGVPHA
                     TARVYYDKKWHTIDGTGFITDITDSNKHQRSAKYSEKHFSTIGEDSYDVVEAGQEPKA
                     ERNYMIIDSNYESWAMKQKTADLLLFNKEKSLVGLDHIAYVEPTYITENKKNHLLDIY
                     KALKGKVEEAKATDTDDKDDKQEGYNRVLQFVNSDIDKLSTLSKITEEEFKALENSMD
                     LARVFLGQMNAKAGKEFSEGETYQSYLKNRQKNNTNSDDDRNRNELQENQANSDEVSQ
                     NSKDASAPSVNSAQHSEELEGTPSTQETISAAPSQQTPVAPKALQAKTELEDKTETSS
                     GSFERESNEVSANTVLSPEITKNTGSLASVESRVEEAVDRTTPSEKVSAESEEVEKTI
                     VSEVSLDASLANENKNSDAQEDTPRLEKSAEVVTTSQEVAEAPVSAPQVSSEIPTSPE
                     TNSTEAVTTSQEMAESRVSAPQVSFVSPTLEVPKNERLDEKADATMPNGVGANTHEAG
                     SVPTSDIYVQDAGSDTVPQPYSLSATLFEEAISTVEPVGVATSSQERSAVAGKVKVPA
                     SLERSNNSVEEEKVVDSNATIEKRATEKKEVLTSENVLNSLVTIWVALSTSFFMRYFS
                     RGK"
     gene            6781..9306
                     /gene="secA"
                     /locus_tag="ACHU66_000461"
     CDS             6781..9306
                     /gene="secA"
                     /locus_tag="ACHU66_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR3388771.1"
                     /translation="MVTNVLRSLIENDKGELRKLEKMADKVFSYADEMEALTDEQLQA
                     KTAEFKERYNNGESLDDLLYEAYAVVREGARRVLGLYPYKVQVMGGIVLHNGDVPEMR
                     TGEGKTLTATMPVYLNALSGQGVHVVTVNEYLSTRDATEMGELYSWLGLSVGINLAAK
                     SPLEKREAYNCDITYSTNSEIGFDYLRDNMVVRAEDMVQRPLNYALVDEVDSILIDEA
                     RTPLIVSGAQGSETNQLYFLADNLVKSLTTEDYIIDIPSKTIGLSDSGIDKAEKFFKL
                     DNLYDIENVAITHFLDNALRANYIMTYDIDYLVNEDQEVMIIDPFTGRTMEGRRYSDG
                     LHQAIEAKEGVPVQNESKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVV
                     PIPTNRPIARVDHEDLLYPSLEYKFNAVIADVKRRYEKGQPVLVGTVAVETSDLISQK
                     LVAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGPGVRELGGLC
                     VIGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERIKVFMERMNLT
                     EEESVIKSKMLTRQVESAQKRVEGNNYDSRKQVLQYDDVMREQREIIYRQRQDVITAD
                     RDLAPEIKAMMKRTIERQVAGHFLGSKDEAIDGIIKFAHANLVEDDTLSKATFEAMNQ
                     KEIVEELYERALRVYDSQVKKLRDEERVREFQKVLILRVVDNKWTDHIDALDQLRNAV
                     SLRGYAQNNPIVEYQSEAFTMFNDMIGAIEFEVTRLMMKAQIHDNIERERTSQEAHTT
                     AVKNIMPNQSHAIQENVSFEGVDRNDPCPCQSGKKFKNCHGRK"
     gene            9421..9780
                     /gene="acpS"
                     /locus_tag="ACHU66_000462"
     CDS             9421..9780
                     /gene="acpS"
                     /locus_tag="ACHU66_000462"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR3388772.1"
                     /translation="MIVGHGIDLQEMEAIESARIKHQGFPKKILTEKEFERYQSLSGR
                     RQLEYLAGRWSVKEALTKALGTGIGKIGFHDIEILNTSKGVPYVTKSPFDGNIWLSIS
                     HSGNFVQASVILEEKDD"
     gene            9773..10876
                     /gene="alr"
                     /locus_tag="ACHU66_000463"
     CDS             9773..10876
                     /gene="alr"
                     /locus_tag="ACHU66_000463"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR3388773.1"
                     /translation="MIESEHRPTQIRVNLDAIAENFEQVMTNLPHKTEAFAVVKANAY
                     GHGAVAVAKKLSSQAAGFCVSNLDEALELRKAGIEHPILILGVVPVRFLPVAHQLNIS
                     VTVASLDWLQDAKDLEADLSGLTVHLKLDTGMGRIGFRKIADLLQAIAILEELEYDIE
                     GVYTHFATADEVDQAHFEYQLSIFKEFLDVLPITPRWIHASNSATSIWHADTVFNLVR
                     LGNILYGLNPSGRVLELPFDVQPALSLVSEIVHVKQVETGTTIGYGATYHSADSEWIA
                     TVPIGYADGLVRSLQGLSVLVDGQACEIVGRISMDQITIRLPHAYPLGQKVTLIGQDG
                     DKTISVQEWADCIGTINYEVVCLLTDRLPRIFE"
     gene            complement(11632..12987)
                     /locus_tag="ACHU66_000464"
     CDS             complement(11632..12987)
                     /locus_tag="ACHU66_000464"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFR3388774.1"
                     /translation="MKVIKFGGSSLASAEQLEKVFNIVQSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYKEYVNGKDVTASQEWIINRYQAMVDELGFTANSMKKIADSIISLAS
                     LPIDDNEFLYDAFLAAGEDNNAKLIAEYFTHRGLPARYVHPKKAGIIVSSEPGNARIL
                     PSSYDKIEELRDTDEVLIIPGFFGVTVDNQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIKNPHSIKELTYREMRELAYAGFSVLHDEALLPAYRGRIPLVIKN
                     TNNPTHPGTRIVHKHTEQTVPVVGIAADDGFVSINLSKYLMNREVGFGRKVLQILEDL
                     NIRWEHMPTGIDDLSIVVRERELTPIKEEEILRQLNTKMEVDKAEIEHGLSIIMIVGE
                     NMKSHVGVTATATTALSKQNVNLAMISQGASEVSVMFVVKTEEKKRALHALYEAFFNE
                     Q"
     gene            13164..13805
                     /locus_tag="ACHU66_000465"
     CDS             13164..13805
                     /locus_tag="ACHU66_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFR3388775.1"
                     /translation="MVKAIIFDMDGVLFDTETFYFQRRADFLATKGLSVDHLDPSIFV
                     GGRASQIWQRILGDDYDNWDVPALEEEYRVYKEKRPTPYAERIFPEVKAVLERLTIQG
                     IPVVLASNTDRSEIERALLDAGIRFHFSHVFSAMDCEAPKPDPTVYMKAASATGVAKS
                     DILVFEDSAKGIEAAKKAGLTVWAIRDQHYGIDQSKADKLVDNLEEAFDLLRF"
     gene            14244..15035
                     /locus_tag="ACHU66_000466"
     CDS             14244..15035
                     /locus_tag="ACHU66_000466"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HFR3388776.1"
                     /translation="MAYQTIRYEVIDSVAILTLNRPEVANGFNIPMCEEILEAIRLAE
                     GDASVKILQIQAQGSIFSVGGDLVEMKRAVDDDDIASLVKIAELVNDISFAMKKLSKV
                     VIMVTDGAVAGAAANMAVAADFVIASSKTKFIQAFVGVGLAPDAGGLFLLSRAIGANR
                     ASHLAMTGEGLSADKALEYGIVYKLAEPEKLEKTVAQVIKKLLRGSVNSYAAIKQLTW
                     ESQFKDWESYRRLELDLQETLAYKEDFKEGVRAHAERRRPVFTGD"
     gene            15115..15549
                     /locus_tag="ACHU66_000467"
     CDS             15115..15549
                     /locus_tag="ACHU66_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFR3388777.1"
                     /translation="MEDPKVNEYLTAIFNNVLVIEETSLRGSRFNDISIKEMHTIDVI
                     GSIDGATPSDVARTLMVTLGTVTTSLNNLERKGYVERIRSTKDRRVVHLYLTKKGRLV
                     YRLHQRFHNEMVKQITDGMDETEYQVMKKGLMKLYHFLEDLK"
     gene            15546..16523
                     /locus_tag="ACHU66_000468"
     CDS             15546..16523
                     /locus_tag="ACHU66_000468"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR3388778.1"
                     /translation="MRNHAKISQVAHYLPKKIVTNDDLAQRMETSDEWIRSRTGIGQR
                     HIVTGETTSDLASQVARKLLEKSQLDASEIDFIIVATITPDASMPSTAAMVQAAIGAK
                     KAFAYDLVAACSGFVFALSTAEKFLASGVYKRGLVIGAETLSRSVDWSDRSTAVLFGD
                     GAGGVLLEACEQPTFLAEILRTDGGRGASLTAGIDQKETPFSTQSRQQPFIQMEGRAI
                     FEFATRDVTATMAELLEQADMTVDRVDYFLLHQANIRILDKMARKLGVVREKFPANMD
                     KYGNTSAASLPILLSECVESGMLRLDGSQTVLMAGFGGGLTWGTLLLQL"
     gene            16583..16807
                     /locus_tag="ACHU66_000469"
     CDS             16583..16807
                     /locus_tag="ACHU66_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR3388779.1"
                     /translation="MAVFEKVQEIIVEELGKDAEEVTLTTTFEDLDADSLDLFQVISE
                     IEDAFDIQIDTEEGLTTVGDLVAYVEEKTK"
     gene            16978..17943
                     /gene="fabK"
                     /locus_tag="ACHU66_000470"
     CDS             16978..17943
                     /gene="fabK"
                     /locus_tag="ACHU66_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HFR3388780.1"
                     /translation="MKTKITELLDIKYPIFQGGMAWVADGDLAGAVSNAGGLGIIGGG
                     NAPKEVVKANIDKVKSITDKPFGVNIMLLSPFADDIVDLVIEEGVKVVTTGAGNPGKY
                     MERLHAAGITVIPVVPSVALAKRMEKLGVDAVIAEGMEAGGHIGKLTTMTLVRQVVEA
                     VSIPVIAAGGIADGAGAAAAFMLGAEAVQVGTRFVVATESNAHQAYKEKVLKAKDIDT
                     TVSASIVGHPVRAIKNKLSSAYAAAEKDFLAGKISADAIEELGAGALRNAVVDGDVTN
                     GSVMAGQIAGLVSKEESCEDILKDIYYGAAKVIREEASRWASVGE"
     gene            17945..18865
                     /gene="fabD"
                     /locus_tag="ACHU66_000471"
     CDS             17945..18865
                     /gene="fabD"
                     /locus_tag="ACHU66_000471"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR3388781.1"
                     /translation="MTKIAFLFAGQGSQTVGMARDLYEYYPIVRETFDQASQILGYDI
                     RDLIDKQEDKLHQTRYTQPAILTTSVAIFRLLEEEGIQPDMVAGLSLGEYSALVASGA
                     LAFEDAIALIAKRGEYMETAAPAGTGKMVAVLNTDVNLIEEICSTVTSGIVSPANYNT
                     PAQIVIGGEVAAVDEAVELLKEAGVKRMIPLNVSGPFHTALLRPASEQLAQALEQVEF
                     ADFQVELVGNTEAKVMKKEDIKSLLTRQVMEPVRFYESIATMQEAGVTNFIEIGPGKV
                     LSGFVKKIDKTAHVVAIEDIEGLQIVLNKS"
     gene            18875..19609
                     /gene="fabG"
                     /locus_tag="ACHU66_000472"
     CDS             18875..19609
                     /gene="fabG"
                     /locus_tag="ACHU66_000472"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011225529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFR3388782.1"
                     /translation="MELTNKNVFVTGSSRGIGLAIAHKFASLGANVVLNGRGQLGQDL
                     LDSFADYGVKVLAISGDISSAEDAKRMVAEAIETLGSVDILVNNAGITKDGMALRMTE
                     EDFDTVLKVNLTGTFNMTQAVLKPMTKAREGAIINLSSVSGLIGNAGQANYSASKAGV
                     IGFTKAIAREVAGRNVRVNAIAPGFIQSDMTDVLSDKIKEAMTAQIPMKRFGATEEVA
                     DVAVFLAKQEYLTGQVIAVDGGLTMR"
     gene            20003..21238
                     /gene="fabF"
                     /locus_tag="ACHU66_000473"
     CDS             20003..21238
                     /gene="fabF"
                     /locus_tag="ACHU66_000473"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR3388783.1"
                     /translation="MTKLNRVVVTGYGLTSPIGNTPEEFWDSLKAGKIGIGKITKFDT
                     SEYSVHNAAELKDFPFDKYFVKKDTNRYDNYSLYALYAAKEAIANAQLDTETVDSDRF
                     GVILSTGIGGILEIEEQVARMNEKGPKRIRPMALPKALPNMAAGNIAMQVGANGVCKC
                     IITACASSNDALGEAFREIKFGFQDVMLAGGAEAAITPFAIGGFQALTAMSTTEDPER
                     ASIPFDKDRNGFVMGEGSAVLVLESLEHAEARGATILAEIVGYGNTCDAYHMTSPHPE
                     GLGAIKAMKLAISEAGLEPADIDYINAHGTSTPANEKGESQAIVSVFGKNTPVSSTKS
                     FTGHLLGAAGAVEAAAVIEAMRHSYAPKTAGTTELSEDIEADVIYGQGRDMEIRHAIS
                     NTFGFGGHNSVIAFKRWEA"
     gene            21239..21715
                     /gene="accB"
                     /locus_tag="ACHU66_000474"
     CDS             21239..21715
                     /gene="accB"
                     /locus_tag="ACHU66_000474"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR3388784.1"
                     /translation="MNITEIKDLMSQFDQSSLREFSYSNAGETLHFSKNQQATVAPSP
                     AVEAPLAPVTPASPALVPAVEMSEPAPAEAGASPVAEGAVVESPLVGVAYLSPAPDKP
                     AFVAVGDTVKKGQTLMIIEAMKVMNEVPADRDGVVTEILVANQDVVEFGQGLVRIK"
     gene            21712..22134
                     /gene="fabZ"
                     /locus_tag="ACHU66_000475"
     CDS             21712..22134
                     /gene="fabZ"
                     /locus_tag="ACHU66_000475"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR3388785.1"
                     /translation="MIDIVKIKEALPHRYPMLLVDRVLEVSEDEIVALKNVTINEPFF
                     NGHFPDYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     IMTATFVKRRGTIAVVEAKAEVDGKLAASGVLTFAIGK"
     gene            22431..23804
                     /locus_tag="ACHU66_000476"
     CDS             22431..23804
                     /locus_tag="ACHU66_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR3388786.1"
                     /translation="MFEKILIANRGEIAVRIIRAARELGIATVAVYSEADKEALHTML
                     ADEAVCIGPARSTDSYLNMQAVISAAVVTGAQAIHPGFGFLSENSKFATLCEEVGIKF
                     IGPSGTVMDTMGDKINARAEMIKAQVPVIPGSDGEVLTSQEALAIAEKIGYPVMLKAS
                     AGGGGKGIRKVEKAEDLVPAFESASSEAKAAFGNGAMYMERVVYPARHIEVQILADQH
                     GHVIHLGERDCSLQRNNQKVLEEAPSIAIGQTMRERIGQAAVRAAQSVGYENAGTIEF
                     LLDEAKGEFYFMEMNTRVQVEHPVTEFVTGVDIVKEQIKIAAGQELSVRQEDVRITGH
                     AIECRINAENPAFNFAPSPGKISNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKVI
                     VHGENRFEALMKMQRALYELEIDGVVTNTDFQLDLISDKRVVAGDYDTAYLMEEFLPR
                     YQEDLKK"
     gene            23819..24685
                     /gene="accD"
                     /locus_tag="ACHU66_000477"
     CDS             23819..24685
                     /gene="accD"
                     /locus_tag="ACHU66_000477"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR3388787.1"
                     /translation="MALFRKKDKYIRINPNRSRIESAPQAKPEVPDELFSKCPACKEI
                     LYKKDLGPEKTCQHCSYNFRITAQERLALTVDDGSFEELFTGIETKNPLDFPNYLEKL
                     AATRQKTGLDEAVLTGKATIGGQPVALGIMDSHFIMASMGTVVGEKITRLFELAIEER
                     LPVVLFTASGGARMQEGIMSLMQMAKISAAVKCHSNAGLFYLTVLTDPTTGGVTASFA
                     MEGDIILAEPQTLVGFAGRRVIESTVRENLPDDFQKAEFLQEHGFVDAIVKRQDLPAT
                     IGRLLRMHGGVR"
     gene            24682..25455
                     /locus_tag="ACHU66_000478"
     CDS             24682..25455
                     /locus_tag="ACHU66_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR3388788.1"
                     /translation="MTSDVARMIRLARDQARLTTLDYATQLFDDFIELHGDRNFRDDG
                     AIVGGIGILAGQPVTVIGIQKGKNLQDNLKRNFGQPHPEGYRKALRFMKQAEKFGRPV
                     VTFINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVAD
                     QVWMLENSMYAVLSPEGFASILWKDGSRAMEAAELMKITSYELEKLQVVDKVILENGR
                     ATKEVLADIKENLVSQLAQLQALPLDKLLENRYQRFRKY"
     gene            25519..25992
                     /locus_tag="ACHU66_000479"
     CDS             25519..25992
                     /locus_tag="ACHU66_000479"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR3388789.1"
                     /translation="MLVKADLSNAEELLPIQHRAFAALYKTYQDQYNPAIETMDYFQS
                     RFARPNCSYYKIIEEEHTVGLVRTTVAEDAEQGWLGLIGIDPDHRGKGYGHKAMLELE
                     SLYPTVRRWVLCTVLQESKLVSFYEKLGYKAIKTEPEQEGMDMVYMEKWIGDSDA"
     gene            25985..26617
                     /locus_tag="ACHU66_000480"
     CDS             25985..26617
                     /locus_tag="ACHU66_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388790.1"
                     /translation="MLEQCRRYLDYQGVHYCKPEKAGALAGDMIALKNVAQAARKEFQ
                     TLSLVLAEHVSPFVPERVSQWMNQAQICRPHFWTYYRFPSASLTDSALAIRLYGKGTE
                     AGISVEVSFIERKKVETSLLQQNRVLNCSIAAPLYYWVQEGQVFYRLDGTEENRQSLL
                     QAVEAGRIRKVLVKYDVPFRTVASEEELLERLIQGFSLLEPYLKMTHLAK"
     gene            26795..27460
                     /locus_tag="ACHU66_000481"
     CDS             26795..27460
                     /locus_tag="ACHU66_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFR3388791.1"
                     /translation="MEKLGQSLKWLGRKIWLLLRIFGLLVLAVLPDLILEGSKSQVTL
                     VIVGLVTLGLLVFFWWRAEKNNLPVWDKNVLSWDGLAIVILAFATMQLTDMLGFYLLE
                     LQGATTTANDASIIESLKGVPFGLALLTTAFLPAIAEEVILRGYFFKKLFGSQAVVGI
                     IVSSLLFGALHGPTDLASWLIYGGGGLIFCVLYHKTGYLIYPIAVHFINNAWSVVALY
                     YFQ"
     gene            complement(27540..28466)
                     /locus_tag="ACHU66_000482"
     CDS             complement(27540..28466)
                     /locus_tag="ACHU66_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR3388792.1"
                     /translation="MNLSKEKLLFHHYYANWLRIYKEGAIREVTLKKYRMTLQWLIRL
                     APSLLLEDLNRVRYQELINSYAKTHERQTTMDFHHQLKGAILDAVDEGIIERDPTRKV
                     IIKGRLPRKKKTKYLNQFELHQLIQELRLGEELNWDWLILLIAKTGIRFSEALALTTQ
                     DFDFHRQLLTIDKTWNYKGDGGFLPTKNKSSIRKIQLDWQTSTQFANLLQHTQPDDLL
                     FVKKERIYNSTINAILEKRCLSANLPVVTIHALRHTHASILLFAGVSIASVARRLGHA
                     SITTTQKTYLHIIRELENQDVDLIMRSLSGLC"
     gene            28588..29163
                     /locus_tag="ACHU66_000483"
     CDS             28588..29163
                     /locus_tag="ACHU66_000483"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR3388793.1"
                     /translation="MKLEELALFTGGSLQVRLDTTSWEDARDYILYNQQHHQLDGYEV
                     IEEVIDSRTVTTDREVTLLEEGDLLFSLLSGKAVLVRAEHAGLLYTQNYIKIEPIAKL
                     DKAFLLYLINESSAIRRQFYQSLQGSEVMKYTVKQLKSLQLGALPPLDSQQKLGKIYL
                     DGLALRQKRQAYAQGDFLRLTYLLKEVYVHE"
     gene            29156..32362
                     /locus_tag="ACHU66_000484"
     CDS             29156..32362
                     /locus_tag="ACHU66_000484"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFR3388794.1"
                     /translation="MNELAFETELIQYITTGTITQPEHLEGLEWSVSDSSTLYAVKSK
                     LWTYEPGIKTTEQLWENFRNILWQHNQHRLDKPLSDTEFAQVKQIISDLKTPYEAGQF
                     LYGISGVSELEITLDDGRHTFLTVFDQRKIGGGDTVYQVVNQIRRTPKIPGRRECIFD
                     TTLLINGLPIIQIEEKKDTVDVDKALNQMHQYIQERQYTDIFSTLQILVAIAPNNVRY
                     MANTTAEKFNKDFAFHWQRKSDGSKVRNWKEFADSMLSIPMAHQMATNYMILDGTPQK
                     QMLKVMRPYQVYATQAVIEGIKKVDFELGCNKVGYIWHTTGSGKTITSFKTAWLASRM
                     PKVDKVVFLVDRIALTNQTKDDYLAYDPEAGENSFGSIRETQNTTALARQLTSKDSGI
                     IVTSVQKLDTLVKRKSFKSPDKHIVFIVDEAHRSTGGDSFAGIQKAFKKAAWIGYTGT
                     PNLDDTGASLRTQDIFGPLLHAYTIKNAISDRNVLGFKVDFETTIDQEKLKTTYLPDF
                     YRQKYPKWTSAQIEEKIANLSPDDMDDAVTSSVYDENPEHVRLVVEDVFKHWANRSGS
                     GHYNALFTTHVGGGRASTPMAMMYFREFQRINQLHREQGGLVLKVGVTFALNTQNNDS
                     MVESNTGLLEAITIYNTEFGTSFGLSDVASYTEDLVSRLNRTAKDKNYLDLVIVVDQL
                     LTGFNAPELNTLYVDRTLKGASLIQAYSRTNRVEDMDKKPFGRVVNYRWPAHNEKLMN
                     QALAIYADDSAANLSEAEQKNNNEKNGLVAKPFKEVFAEVKQTVDQLRELTGDFNDIP
                     KSEKKREAMLELLRAYNQGLSKLKQYQPEEVDGVIEGFDYNQPDRFIESLGMSVEEEK
                     LLTTVLANELKRTLAKEQKVSIHQIELKMTHIKEVTIDYDKLTELVELLLNQVHEEKM
                     VEAAATRDELDRFASGLEDLNYADQIRQTGDAIYRGDFPTQDMNFAYPVKGLDSQKII
                     QEASRVYIDKKLLAFRIQWGLTEHIRNQELRELLARHRYGQNDLDDTGHLRTLIKEAS
                     RDYQTWAEGDAVKGLSKIQYRNQLRQALYDLADDLVGQ"
     gene            32470..>33177
                     /locus_tag="ACHU66_000485"
     CDS             32470..>33177
                     /locus_tag="ACHU66_000485"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR3388795.1"
                     /translation="MTKEKSTVPRLRFPGFTDAWKQRKLGEVADFSIKTNSLSRDKLS
                     SYFYEVQNIHYGDILTKYDAILDVCNKELPSIIGSTISDFADALLSEGDIVFADAAED
                     STVGKAIEVRNFKGKNVVSGLHTIVARPKVSYAPYYLGYLINSTAYHNQILPLMQGTK
                     VSSISKANLKSTTVVFPTLPEQEAIGSFFSDLDQLITLHQRKLDDVKELKKALLQKMF
                     PKGNGNDFPELRFPGFTD"
BASE COUNT         9791 a         6218 c         7778 g         9390 t
ORIGIN      
        1 agttttaaca atgttttttg ataaaattta aataaaatgt gataaacgtt tgacatattt
       61 tgtaaaagtg atataataaa accatcgaaa gtttgaaaac actttcaaaa atatacaaaa
      121 aggagatttt tgtaaatgga ttataaaaaa gtttctgccg aagtgatcga agcggttggg
      181 aaagataatc tagttgcggc agcacactgt gctactcgtc ttcgtttggt tctgaaggat
      241 gaatccaaag tgaaccaaaa agccctcgat gataatgcag atgtcaaagg gacattcagt
      301 acgaatggtc agtatcaagt tatcatcgga cctggtgatg taaactttgt atatgcagag
      361 ctcatcaagg ctacaggctt aaaagaagtt tcaacagatg atttgaaaca gattgcagcg
      421 caagataaga agttcaatcc attgatggac ttgatcaagc tcttgtcaga tatctttgtg
      481 ccaatcattc cggctctcgt tgctggtggt ctattgatgg cgcttcgtaa cttcttgact
      541 tccccagatt tatttggtcc aaaatcaatt gaggaaatgt atcctgctat ccaaggcatt
      601 tctgcgatga ttcaattgat gtcagcagct ccattcatgt tcttgccagt ccttgtaggt
      661 atctccgctg cgaaacgctt tggtgcaaac caattcctcg gtgcggctat tggtatgatc
      721 atgaccactc cagatttggg tggtaaagaa gctttttggg atatttttgg tttccaagta
      781 gctcaaacaa actatgcata tcaggttatt ccggttcttg cagcagtatg gcttcttgcg
      841 acgcttgaaa aattcttcca caagaagttg ccatcagcag ttgactttac attcacacca
      901 ttattgtcag taatgattac aggtttcttg acatttacag tcatcggtcc tgtgatgtta
      961 accgtatctg atgcgattac taacgcaatt gtttggttgt acgatacaac tggtgcgttg
     1021 ggtatgagta tctttggtgg tacttactca ttgatcgtaa tgactggtct tcaccaatca
     1081 ttcccagcca tcgagacaca acttttgtct gcttacaaca ataatggtac aggatacgga
     1141 gactacatct ttgtagttgc ttcaatggct aacgttgcac aaggtgctgc aactcttgct
     1201 atctatttct tgacaaaaaa tgcaaaaaca aaaggtttgt ccggttcagc agcagtttct
     1261 gccttccttg gaattacaga accggccctc ttcggggtta acttgaagta cagattccca
     1321 ttcttttgtg ctcttgcagg ttcggctatc ggtgccctct ttgcaggttt gactcatgtt
     1381 atcgctgtat ctcttggggc agcaggtttc attggattcc tctcaatcaa agcatcttct
     1441 atcccaatgt atgtagttgc tgaggtgatt gcttttgctg ctgcctttgc ctttacttac
     1501 gtttatggca agacaaaagc atcagcagtt ttcgctgatg aagcagcaac agcagctgcg
     1561 gcagcagtga cagcgtctgt agttgaaact tcagaagaag ttgaagaaac tgcagaaatc
     1621 tttactgcac aagaaactct tgtatctcca ctttcaggtg acgttgttgc ccttgaaaat
     1681 gttaatgacc cagtcttctc atcaggagct atgggtaaag gtttggctgt taaaccgtct
     1741 gagggtgttg tttacgctcc agcagatgca gaagtaacaa ttgcttttga aacaggccat
     1801 gcttacggat tgaaaacagc gtctggtgct gaacttttaa ttcacatcgg tattgatact
     1861 gtatcaatga acggtaatgg atttgaaaaa ttggtggcag caggtgataa agtaaaagct
     1921 ggtactccga ttgctaaatt tgatgcaact aagattgcag aagcaggtct tgatgataca
     1981 accatgatca tcgtgaccaa cacagctgac tttgcagaag tatctccact tgcagaggga
     2041 acaatcgctc acggtgtaga attcttgaaa gtagctaagt aatcttatat aaaatcgatt
     2101 tgtagagtct gcctctacta cataattaac aggaaaaagg ctttcagcct ttttcttctg
     2161 tttgcttata tgttataata aaagtgataa aaattttgga gacaatcaaa tgacaaaatt
     2221 atatggtagt ttagaagctg gtggtacaaa gtttgtttgt gcagtaggtg atgagaactt
     2281 tcaagttgtt gaaaaaacgc aatttccaac gacaactcct tatgaaacga ttgagagaac
     2341 agttgaattc ttcaagcgct atgaagatcg tttggcaggt attgcgatag gttctttcgg
     2401 tccaattgat attgatcaaa actcacagac ttatggttac atcacttcga caccaaaacc
     2461 acactggtca aatattgatt tacttggtct gattgctaag gaatttaaca ttccattcta
     2521 cttcacgaca gatgtgaatt catcagcttt tggtgaaacc ctggttcgta aaggggtaaa
     2581 gagcttggtt tactatacaa tcggtacagg tattggtgca ggtgctattc agaatggtga
     2641 gtttatcggt ggcttgggtc ataccgaagc gggtcatact tacgttgccc tacaccctta
     2701 cgatgtaaaa catgaattta aagggatgtg tccattccac aatgggtgtt tggaaggctt
     2761 agctgcaggt ccttctcttg aaggccgtac aggtattcgt ggtgaattaa tcgagttaaa
     2821 ttcagacgtt tgggatgtgc aggcttacta cattgctcaa gcagcagtac aggcaacctt
     2881 gctttatcgt ccgcaagtca ttgtatttgg tggaggagtt atggctcaag agcacatgct
     2941 taatcgtgta cgtgagaagt ttgtcggttt gatgaacgat tatctcccaa caccagatgt
     3001 caaagaatat atcgtaacgc cagctgttgc tgaaaatggt tctgcaactc ttgggaattt
     3061 tgctttggca aaacaggtgt caagaaattc ataattgttt atgtgaacag attcatttgt
     3121 actttggcga tagatatatt gaagaagaat attgggtgaa gttgttttat tatcacaata
     3181 acttgacagt gaggtgagag tttttatgta actctcacct ctttttacct aaaaaactgt
     3241 attcataaga gatagccgtt tgttaatcaa ggcagtcttt gggagtagga aagaatcaga
     3301 ccggcttaaa aagagttcgt caacaactct ttaatttaag tttttgagcc gaaacagcgc
     3361 ctcaaaaaac taacgatgac tataaaagat tagagtttgc cattgttgga gttcatcggg
     3421 tatgaaaaag agaaacgagt tccacaactt ttgatgatta ttcataagca aggttgaaaa
     3481 tctttttatt ggcgatagtg tagtgatatt ttttaaccag aggaaagttg ttgtttctca
     3541 attgtatgaa aacgctatat ctaaagtaat gatgcggaga cactaaagtc tataaaaaca
     3601 ggaaaagcac agatagatag cagaaaaaag atagttaaaa gtgttacaat tgggatgcaa
     3661 attttaggct gggaaattat tcttagaagt atttatatgg aggacttatg attccaattc
     3721 aacagcatgg taagcgctgg cttattttga cacttaccct ttctacttca gttcttgcgc
     3781 ttgcatctat cggagcatca caaccggttg tttttgcaga tgaaaactca catttacaat
     3841 cacaaaaaaa cattaataaa atagaggttc tggactggaa aacattttct aaaaaattga
     3901 gcgaatactc ctcggaccaa cgacaatttc atgttttgaa attgggattt gaaaatcgtc
     3961 ttgggacatt atcaacttgg gatgaattgg aagaatttgg taaaaacaac aattttttgg
     4021 taatcaatgg taaagttact caaaatattc atgactttcc acatatcctt gttatgaaca
     4081 agggtgatgt tattgctcat aatgaagaag attatcataa tcagatgaga gagttgaggt
     4141 tttcgggaaa tggcgactta cataatagta tggagccaaa acggattcat gcactattta
     4201 agattgaact tgactccaat aaacggcagt tattaaatgc tgcgggtcta ggaactgctg
     4261 agaatagttt gaaaaatatt aatggaatga ctatttactc gcatggtctt acggttgata
     4321 ataagtacta tgaggattat agtaaatata ctcataattc agttaaaaat ataaacgtta
     4381 ccaaagagcg ttttatagca aatgatgact tgattcataa gttgatagag tcctcagaag
     4441 ctatgaagca atcaagtgaa cgcgataaag ttaaggcatt tgttcagtat gttgccaatc
     4501 atacaacata tgattgggaa gctgctaata aggctgttca aaattatgca gacatcaatt
     4561 actacttagg atcagatttg ttcgcggtta ctgaacgtca gaaagcgatg tgtgtaggtt
     4621 tcagcactac tgcggcccgt gcatttaata tgttagggct tcctgcttat gttgttgttg
     4681 ggaaaaatgc agaaggagtc ccgcatgcta cagcgcgtgt ttattatgat aaaaagtggc
     4741 acaccattga cggtacaggt tttattacag atattactga ttctaataag catcagcgct
     4801 cagctaaata tagtgaaaaa cacttttcta ctatcggtga agatagttat gatgttgttg
     4861 aggcaggaca ggagcctaag gcggagcgta actacatgat tattgatagt aattatgaga
     4921 gttgggctat gaaacaaaaa acagcagact tactgttgtt caacaaagaa aagtccttgg
     4981 ttggtttgga tcatattgca tatgttgagc caacttatat aacagaaaat aagaaaaatc
     5041 atttgctaga catctataaa gctttgaaag gtaaggtaga agaagcgaaa gcaacggata
     5101 cagatgataa agatgataaa caagaggggt ataatcgtgt tttacaattt gtcaattctg
     5161 atattgataa attgagtact ctctccaaga ttacggaaga agaatttaaa gcactcgaaa
     5221 attcaatgga tttagctcga gtgttcttgg ggcaaatgaa tgcgaaggct ggaaaagaat
     5281 tttcggaagg agaaacttat caaagttatc taaagaatcg tcagaaaaat aacacaaatt
     5341 ctgatgatga tagaaaccga aatgaattgc aagaaaatca agctaattct gatgaagtca
     5401 gccaaaattc caaggatgct tcagctccaa gtgttaattc tgcacagcac tctgaggagc
     5461 ttgaaggaac tccttcaaca caggaaacaa tctcagctgc tccttctcaa caaacaccag
     5521 tagctcctaa ggcactacaa gctaaaactg agttagagga taaaacagag acttcatctg
     5581 gttcctttga gagagaatcg aatgaagtgt cggctaatac agttttgtct cctgaaataa
     5641 ctaagaacac gggaagtcta gcttctgtag aatctcgagt ggaggaggct gtggatagga
     5701 caactccatc agaaaaagtt tctgctgaat cagaagaagt agaaaaaaca attgtatctg
     5761 aggtatcatt ggatgcttca ttagcaaatg agaacaaaaa ttctgatgct caagaagata
     5821 ctccacgttt ggagaaatct gccgaggtgg taacaactag tcaagaagta gctgaagcac
     5881 cggtttctgc tccacaagta agttctgaaa tcccaacttc accggaaact aattctacag
     5941 aggcagtaac aactagtcaa gaaatggctg aatcacgggt ttctgctcca caagtgagtt
     6001 ttgtatcgcc tactttagag gttcctaaaa atgagcgttt agacgagaaa gcagatgcaa
     6061 ctatgccaaa tggagttgga gcaaatactc atgaggcagg ttctgttccg acatcagata
     6121 tttatgttca agatgctggt tcagacacgg tgcctcaacc ttactcttta tctgcgacat
     6181 tgtttgaaga agcaatttca acagttgagc ctgtaggtgt tgcaacttct tctcaagaga
     6241 gaagtgcagt agctggcaag gtgaaagtgc ctgcctctct tgaacgtagt aacaattctg
     6301 ttgaggagga aaaagtagtt gattcgaatg caacaattga gaaacgagcg acagagaaaa
     6361 aagaggtcct tacttcggaa aatgtactta atagcttagt aacaatttgg gtagctttat
     6421 ctactagttt cttcatgaga tatttttctc gaggaaaata atatattaac acaggttgaa
     6481 gataatattt atctttgtgt agaaaattat tcatgtgagc ttttattaac cgaataatat
     6541 gacaactgag cttttaatga atttctaaat ctccgatata tcgcagattc ctcatttaaa
     6601 attttataaa tttttcacct cctaggagtt attgtgacta ggaggtgttt tgtggtaaaa
     6661 atagtaatat taagctagca ttttcactta tttaggctgt atcctagtgc caaaccttga
     6721 aaattgccta gttttctgct acaatatatt attgactaaa actgaaatag gatgaatgat
     6781 atggtaacaa atgtacttcg ttctctcatt gaaaatgata agggagagct aagaaaactt
     6841 gagaaaatgg cagacaaggt cttctcgtat gccgatgaaa tggaagcgct gactgatgaa
     6901 cagttgcaag ctaagacagc agaatttaag gagcgataca ataacggtga atccctcgac
     6961 gatcttttgt acgaagctta tgcggtagtc cgtgaaggag ctcgtcgcgt attgggtctc
     7021 tatccctaca aggttcaggt tatgggagga atcgttcttc acaacggtga cgttccggaa
     7081 atgcgtacag gtgaaggtaa aactctgact gcaacgatgc ctgtttattt gaatgccttg
     7141 tctggtcaag gtgttcacgt agtgacggtt aacgagtatc tttctactcg tgatgcgact
     7201 gaaatgggtg aattgtattc gtggcttggt ctatcagttg gtatcaactt agctgctaaa
     7261 tcacccttgg aaaaacgtga ggcctataac tgcgatatta cttattcgac caactcagag
     7321 attggttttg actatctccg agacaatatg gttgttcgcg cagaagacat ggttcaacgt
     7381 cctctcaatt atgccttggt cgatgaagtt gactcaatct tgattgacga agctcgtacg
     7441 ccgttgattg tatctggtgc acaaggttcg gaaacaaacc agttgtattt cttggcagat
     7501 aatcttgtta aatctttgac aacagaagat tacatcattg acattccatc taaaacgatt
     7561 ggtctgtctg attcaggtat tgacaaagca gagaaattct tcaagttaga taacctctac
     7621 gatattgaaa atgtagccat tacccatttc ttggataatg cacttcgtgc caactacatc
     7681 atgacctatg atattgacta tctggtcaat gaagaccaag aggtcatgat tatcgatcca
     7741 tttacaggtc gtacgatgga aggtcgtcgt tattctgatg gacttcacca agccatcgaa
     7801 gctaaggaag gtgtaccggt tcaaaacgag tctaagacaa gtgcctctat tacctaccaa
     7861 aaccttttcc gtatgtataa aaaattatca gggatgacgg gtactggtaa aacagaagag
     7921 gaagaattcc gtgaaatcta caacatccgt gtcgtaccaa tcccaaccaa ccgcccaatc
     7981 gcgcgtgttg accacgaaga tctactctat ccaagtctag aatacaaatt taatgccgtt
     8041 attgctgatg tgaagagacg ctatgagaaa gggcaacctg tcttggtcgg tacagttgcg
     8101 gttgaaactt ctgatttgat ttctcaaaaa ttggtagctg caggtgtccc tcacgaagta
     8161 ttaaatgcaa aaaaccacta tcgtgaagcg cagattatta tgaatgctgg tcagcgtggt
     8221 gcggtcacta ttgcgaccaa catggcggga cgcggtaccg atattaagtt aggtccaggt
     8281 gtacgtgaac ttggtgggct gtgtgttatt ggtactgagc gccatgaaag tcgtcgtatt
     8341 gataaccagc ttcgtggacg ttcaggtcgt caaggtgacc caggtgaatc acaattctat
     8401 ctatctctcg aagatgactt gatgaaacgc tttggttcag aacgcatcaa agtatttatg
     8461 gagcgtatga atctaacaga agaagagtcc gtcatcaagt ctaaaatgtt gacacgtcaa
     8521 gttgaatctg ctcagaagcg tgtggaaggg aacaactacg actcacgtaa acaagtcctt
     8581 caatatgacg atgtgatgcg tgagcaacgt gaaatcatct atcgccaacg ccaggatgtt
     8641 attactgctg accgtgattt ggctcctgaa atcaaagcga tgatgaaacg gacgattgaa
     8701 cgccaagttg cgggtcactt ccttggttcg aaagatgaag ctattgatgg aattatcaaa
     8761 tttgcgcatg caaatctcgt tgaagatgat accttgagta aagctacttt tgaagcaatg
     8821 aatcagaaag agattgtcga agagctctat gaacgtgctt tgagagtcta tgattcgcaa
     8881 gtgaaaaaat tacgcgatga agaacgtgta cgcgaattcc agaaagttct tatccttcgt
     8941 gttgtggata acaaatggac agaccatatt gatgccttgg atcaattgcg caatgcagtc
     9001 agtcttcgtg gatacgctca aaacaatcca atcgttgaat atcaatcaga agcatttact
     9061 atgttcaacg atatgattgg ggctatcgag tttgaagtaa cccgcttgat gatgaaggcg
     9121 caaattcatg acaatattga gcgtgagcgt acaagtcaag aagcacatac gacagctgtt
     9181 aagaatatta tgccgaacca atcgcatgca attcaggaga atgtatcttt tgaaggtgtt
     9241 gatcgtaacg atccatgccc atgccagtct ggtaagaaat ttaaaaattg tcacggccgt
     9301 aaataggaat gacgcatgga ctggacggct gtccagtcct tttgcaattt ttagacaagt
     9361 tactagagtc agttcgttta ttttaataaa tgaaatgact atgcaaaagg agaccgatta
     9421 atgatagtag gacatggcat tgaccttcag gagatggagg cgattgaaag tgccagaatc
     9481 aaacaccaag ggtttcccaa aaagattctc acagagaagg aatttgaacg gtatcaaagt
     9541 ctgtccggtc gccgtcagct agaatattta gctggacgtt ggtcagtcaa agaagcgctg
     9601 accaaggcct tggggacagg tattggtaag ataggatttc atgatattga aattctgaat
     9661 accagcaaag gagttcccta tgtgacgaag agtccctttg atgggaatat ttggctcagc
     9721 attagccatt caggaaattt tgtacaagct agtgtgattt tggaggagaa agatgattga
     9781 aagtgaacat cgacccacgc aaattcgggt taacttagat gcgattgcgg aaaatttcga
     9841 gcaagtaatg accaacttgc cccataagac cgaagcattt gcagttgtta aggcgaatgc
     9901 ttatggtcat ggagctgttg cggtagcgaa aaagttatcc agtcaagcag ctggtttttg
     9961 tgtttcgaat ttggatgaag cattagagtt acgtaaagcg ggtatcgagc atcctatctt
    10021 gattttagga gtggttcctg tacgtttttt accagtagct catcaactca atatcagtgt
    10081 gacagttgct agtctggact ggttgcaaga tgcaaaagac ttagaggctg atttatctgg
    10141 tctaactgtc catcttaagc tagatacagg tatggggcgg attggtttta gaaaaatagc
    10201 cgatttgcta caagcgattg ctatccttga agaattagag tatgacatcg aaggagttta
    10261 tactcatttt gctactgcgg atgaggttga tcaggcgcac ttcgagtatc aactgtctat
    10321 ttttaaagaa tttttggatg ttctaccaat tactccgcgc tggattcatg ccagcaattc
    10381 agcaacaagc atttggcatg ctgatacagt atttaacctg gttcgtttgg gaaacattct
    10441 ttatggactt aatccaagtg gacgagtact agaactcccg tttgatgttc aaccagcctt
    10501 atctctcgta tctgaaattg ttcatgtaaa gcaggttgaa actggcacaa caattggcta
    10561 cggagctact tatcacagcg ccgattcaga atggattgcg accgttccga taggatatgc
    10621 agatggtctc gtacgaagct tacaaggctt atcagtccta gtcgatgggc aggcctgtga
    10681 aattgtaggg cgtatttcga tggaccagat caccattcgc ttacctcatg cttatccact
    10741 tggtcaaaaa gtcactctaa tcggtcaaga tggagataaa acgatctctg ttcaggagtg
    10801 ggctgattgc atcggaacca taaattacga ggtggtttgt cttttgacag atcgcctacc
    10861 tcgaattttt gagtaaacgt gacgataatc cgcctctttc actaggatag aaggctaaaa
    10921 tattagagag gtattgccta gagattgtct ataatacaaa tagcgtcatt tagttgactt
    10981 ttattacttc gacaatcgag gaaatatcgt ttccatattt ccaacttcaa acactccact
    11041 ggattgtttg aactcgcctt gcctgatttt gtaaaagtga cttgctttgc aagcaatctg
    11101 ggggatacga atagtccagc ggactattcg tacctgagcc tgaaaacaaa aaagcgaagt
    11161 gcatcagctg gtcacctaga aataagaaag cattgagtta tgcctgcggc ttttgatgtg
    11221 aacatcgttc actttgtccc cagtctccaa ctatctccca tatagttgga gcgagtgcta
    11281 aaagtaaact aaaagactat aatgagtctg gtcaaaaagt ctaatactct tcgaaaatca
    11341 aaattatccg ttgtcaactt gccttgatga acttcagttc tatcttcgac ttcgtttcct
    11401 aggctacttt tgattttcat tgagtataaa aaataaaaaa atagcttaga attgcatcgt
    11461 cagaaacgtt gattctaagc tatttttgtt tattcaaatt ttaattgcta ctcaatgaaa
    11521 attaaaatca gactagctcc aatggagctt gatactcatc aaatcaagtc aacaacgtct
    11581 ggttttgatt ttcgaagagt atgaagaggg aactgaccga cctagttgtg attattgctc
    11641 gttaaaaaaa gcttcataga gtgcgtgcag agcgcgtttc ttttcttcgg ttttgacaac
    11701 gaacataaca gaaacttcac tggctccctg tgaaatcatt gccaagttga cattttgttt
    11761 agagagggca gtggtagcgg tagctgttac accgacatgg ctcttcatat tttcaccaac
    11821 aatcatgata atagataaac catgttcgat ttcggccttg tcaacttcca ttttcgtatt
    11881 gagttgacga aggatctctt cttctttaat aggagtcaat tcacgctcac ggactacgat
    11941 ggataaatcg tcaataccgg tcggcatgtg ctcccagcgg atattgagat cttcgagaat
    12001 ttgcaacacc ttgcgaccaa aaccaacttc gcggttcatc aagtatttag acaagttaat
    12061 gctgacaaaa ccatcgtctg cagcaatacc gacaacagga actgtttgct cggtatgttt
    12121 gtgtacaatt cgggtaccag ggtgagttgg gttattggtg tttttgataa ccaatggaat
    12181 ccgcccgcga taggcaggaa ggagggcttc atcgtggagg acggagaaac ctgcataagc
    12241 taattctcgc atttcacgat aggtcaattc tttgattgaa tgtggatttt taatgatacc
    12301 aggatgggca gcaaaaatac catcgacatc tgtaaagttt tcatagaggt cagccttgac
    12361 tccggcagca atgatggaac ctgtaatatc cgatccacct cgtgagaagg tacaaatctg
    12421 gttatcaaca gttacaccga agaatcctgg gataatgagt acttcatctg tatcacgaag
    12481 ctcttcgatt ttgtcataac ttgaaggcaa aatgcgagca tttcctggtt cggagctgac
    12541 aataatacca gcttttttag gatggacata gcgtgcgggc aatcctctgt gggtaaaata
    12601 ttcggcaatc aatttggcat tattatcttc accagctgct aagaatgcgt cgtaaaggaa
    12661 ttcgttatca tcaattggta ggctggccaa agagatgatg ctgtctgcga ttttcttcat
    12721 gctgttagct gtgaaaccaa gttcgtcaac cattgcctga taacggttga tgatccattc
    12781 ttgactggca gtcacatctt ttccgttaac atattcttta taatatttaa tcaatgcatc
    12841 agtaacttta gtatcctcgg cattgcgttt tccaggtgca gacaccacaa caaagcgacg
    12901 ttctgaatca gattgaacaa tattgaaaac tttttctaac tgttcagctg aagcaagtga
    12961 gcttccccca aatttaataa ctttcattct catctccttg gatatttttt tctattatag
    13021 cagaatgata tgaaattgtc agtatgttca gacaaatctt gccaagaatt ttatcgaaaa
    13081 acgaacaaga gacaagtatg aaaatttcag aaaaacattt ttttctttca actgtgttac
    13141 aattaaagta ataggaggcg agtatggtaa aagcaattat tttcgacatg gatggtgtac
    13201 tttttgatac agaaacattc tattttcaac gtcgagcgga ttttttagcg acaaaaggtt
    13261 tatcggttga tcatttggat ccatctatat ttgtcggtgg aagggctagt caaatttggc
    13321 aacggatttt aggggatgat tatgataact gggatgtccc tgccttggaa gaggaatatc
    13381 gtgtatataa agaaaagcga ccaaccccgt atgcagagag gatttttcct gaagttaaag
    13441 cggtcttgga gagactaact atccaaggta ttcctgtagt tttggcttca aataccgacc
    13501 gttcagaaat cgaacgtgct ctactagatg ctggtatacg ctttcacttt agtcatgttt
    13561 tctcagcgat ggattgtgag gccccaaaac cagatcccac agtttatatg aaagcagcta
    13621 gtgcaacagg agtagctaaa tcggatattt tagtatttga agatagtgcg aaggggattg
    13681 aagcggctaa aaaagcaggt ttgacagttt gggctattcg agaccagcat tatggaattg
    13741 atcagtcgaa agcagacaaa ttggtagaca atcttgaaga ggcctttgac ttattgcgat
    13801 tttaagaaat gttatatttt agtccaagta ttagttctcg gtgaaaaaat ggtatttcta
    13861 gctcagaatc cagttagatt ttccaaaagt attaacgtga aaaaatgaaa gttgtaaatt
    13921 aaacttagag cctatattta cagttcagac ttgcatattg aggttgtttt ttcaaaatac
    13981 agtcagtatt tcccgcaaaa actactttga tgagtgaaaa agccaaggcg ctttgcgtgt
    14041 gaatataggt tcttagacac gttattttct agcatatcta aattttatat tgcgatgata
    14101 gcccccatct ttttcggacg tcacacttta cttgctagaa aaaaagtgac aattataaaa
    14161 actgtgttac aatggaaaac aagaatttga agtttgatat tcaaatcttt taattatcaa
    14221 actattttga aaggagcaag agaatggcct accaaacaat tcgttatgaa gtaattgatt
    14281 ccgtagcgat tctaacttta aacagaccgg aagtggcaaa tggttttaat attcctatgt
    14341 gtgaagaaat cttagaagca atccgccttg cagaagggga tgcctccgtc aaaattttac
    14401 aaattcaagc acagggctcc attttctccg tgggtggaga tttagttgag atgaagcgtg
    14461 cagttgatga tgatgatatt gcttctttgg taaaaatagc agagctggtg aatgatattt
    14521 cgtttgcgat gaagaaattg tctaaagtag tcattatggt gacagatggg gcagtggctg
    14581 gagccgcggc taatatggca gtggcagctg attttgttat tgcctccagc aagacaaaat
    14641 tcatccaagc ctttgtaggt gttggacttg cgccggatgc gggggggctc tttctactga
    14701 gtcgtgctat tggggctaat cgtgctagtc acttggcgat gacaggagag ggcttatcag
    14761 cggataaggc tttagaatat gggattgttt ataaattggc agagccagaa aaactagaaa
    14821 agacggttgc ccaggtcata aaaaaactct tgcgaggctc agtaaactct tatgcagcaa
    14881 tcaaacaact gacatgggaa agtcaattta aagactggga gtcttatcgc cgattggagc
    14941 tggatttgca agaaacattg gcctataaag aggattttaa agagggagtt cgagcgcatg
    15001 cagaacgacg tagaccagtg tttacgggag attaattttt aaattagtaa aaaaagtttg
    15061 acaatcaaag ttttatggtt tatactttga ctatcaaagt aaaggaggcc tgttttggaa
    15121 gatccaaaag taaatgaata tctaacggcc atttttaaca atgtgttggt gattgaagaa
    15181 acaagtctgc gtggtagccg attcaatgat atttcaatta aggaaatgca cacgattgat
    15241 gtgataggaa gtattgatgg agctactccg agtgatgttg ctcggacttt gatggtaacc
    15301 ttaggtactg ttacaactag cttgaacaat ttggaaagaa aaggatatgt tgagcgtatt
    15361 cgttcgacca aagatcggcg agttgttcat ctctatctga ccaaaaaagg tcgactggtc
    15421 tatcgtttgc atcaacgatt tcacaatgaa atggtgaaac agattacaga tggtatggat
    15481 gaaacagagt atcaggtaat gaagaaagga ctcatgaaac tctatcactt cttggaggat
    15541 ttgaaatgag aaaccatgct aaaatcagtc aggtggccca ctatcttccg aagaagattg
    15601 tgaccaatga tgatttggct caacgaatgg agaccagtga tgagtggatt cgtagccgta
    15661 caggtatcgg tcaacgacac attgttactg gagagacgac gagtgatttg gctagtcaag
    15721 tggctagaaa attactggaa aagtctcagc tagatgctag tgaaattgat ttcatcatcg
    15781 tagcaacgat tactccagat gcttcaatgc catctacggc agctatggtc caagcagcaa
    15841 ttggtgcgaa aaaggctttc gcctatgatc ttgttgcagc ttgttcagga tttgtttttg
    15901 cgctatcaac agcggaaaaa ttccttgctt caggagtcta taagagagga cttgtcattg
    15961 gtgcagagac cctatctagg tctgtcgact ggtcggatcg atcaacggct gttttatttg
    16021 gagatggtgc tggaggagtt ttgctggaag cgtgcgaaca accgaccttt ttagcagaaa
    16081 tcttgcgaac ggatggcggt cgaggtgcga gtctgacagc agggatagac cagaaagaga
    16141 cgccattctc gacacaaagc cgtcaacaac cctttatcca aatggagggc agagctatat
    16201 ttgaatttgc gacgagggat gttacggcaa ccatggcaga gctgctagaa caagctgata
    16261 tgactgtgga tcgtgttgat tatttcttgc tccatcaagc caatatacgt atcctagata
    16321 agatggctcg taaattggga gtggtgcgtg aaaaatttcc agcaaatatg gataaatatg
    16381 gcaataccag cgcagcaagt cttcctattc tcttatcaga atgtgtagag agcggaatgt
    16441 tacgattgga tggaagccag actgttctca tggctggttt cggtggaggc ctcacatggg
    16501 gaactctact attacaactt tagttaattc cttgccctag gcaagattaa aatattttat
    16561 tttccaaaag gagacacata tcatggcagt atttgaaaaa gtacaggaaa tcatcgttga
    16621 agaattgggt aaggatgctg aagaagtaac ccttacaaca acctttgaag atttggacgc
    16681 agattcattg gatcttttcc aagttatctc tgaaatcgaa gatgcattcg acatccaaat
    16741 cgacactgaa gaaggtttga ctactgtagg tgatttggtt gcctacgttg aagaaaaaac
    16801 aaaataatga ctgttaagga aagtgctatt tagcattttt cttaaattaa atactttgat
    16861 aatcaaaaca ttgaaatatc aaactatttt ttgagtgagg gggcaaccct tcacttgttt
    16921 acctaatgat ttgatattca aatgattagg taagcaagaa aaaaagatga gggaattatg
    16981 aagacgaaaa taacagaatt gctcgatatt aagtatccaa tttttcaagg tggtatggct
    17041 tgggtagctg atggagattt ggctggtgcc gtatctaatg caggtggtct aggaatcatc
    17101 ggtggaggaa atgctcccaa agaggtagtc aaggcaaata ttgacaaggt taaatctatc
    17161 acggacaaac ctttcggtgt caatatcatg ctgttgtctc cttttgcaga tgacattgtt
    17221 gacttggtca tcgaagaagg tgtgaaagtt gtcactacag gagcaggaaa tccagggaag
    17281 tatatggagc gtctccatgc tgcagggatt acagttattc ctgttgtacc gagtgtagcc
    17341 cttgctaaac gcatggaaaa attaggggta gatgctgtta ttgctgaagg tatggaagcc
    17401 ggaggccata ttggtaaatt gaccaccatg accttggttc gtcaggttgt tgaagccgta
    17461 tctattcctg ttattgctgc tggaggtatt gcagatggtg caggtgctgc ggcagccttt
    17521 atgttgggag cagaagcggt gcaagttgga actcgttttg tagtagctac agagtccaat
    17581 gcccatcagg cttataagga aaaagtttta aaagctaagg atattgatac aactgtatct
    17641 gcttctattg ttggacatcc tgtgagagcc atcaagaata agttgtcatc tgcttatgca
    17701 gctgcagaga aagatttctt ggcaggtaaa atttcggcag atgctattga agaacttggt
    17761 gcgggagcac tccgcaatgc agttgttgat ggtgatgtaa caaatggctc cgtgatggca
    17821 ggtcaaattg ctggtctggt ttctaaagaa gaatcttgtg aagatatttt gaaagacatc
    17881 tattatggag cagccaaagt gattagagaa gaggcaagcc gttgggcttc ggttggagaa
    17941 taggatgaca aagatagctt ttctttttgc aggtcaaggg tctcagacag tggggatggc
    18001 tcgcgacctt tacgaatatt acccgatagt tagagaaacg tttgaccagg ctagtcagat
    18061 tctggggtat gacattcgcg acctaattga caaacaagag gataaattac atcagactcg
    18121 ctatacccaa ccggcaattt tgacaacttc tgtggctatt tttcgactgc tggaagaaga
    18181 gggaatacaa cccgatatgg tagctggtct atccttggga gaatattcag ctctggtagc
    18241 atcgggagct ttggcctttg aagatgcgat tgccctgatt gctaagcgtg gtgaatatat
    18301 ggaaacggct gcgccagcgg ggactggtaa gatggttgcg gttctcaaca cagatgtcaa
    18361 cttgattgaa gaaatctgtt cgactgttac ctctgggatt gtttccccag ccaactacaa
    18421 cacaccagcc caaatcgtca tcggaggcga ggtggctgca gtggatgagg cggttgaatt
    18481 gctaaaggaa gctggtgtca aacggatgat tccgctcaat gtatctggcc cctttcacac
    18541 ggctctcttg cgtccagctt cggagcaatt ggctcaggcc ttggagcagg tggaatttgc
    18601 ggacttccaa gtagagttgg tcggtaatac ggaagccaag gttatgaaaa aagaggacat
    18661 caagtccctc ttgacccgtc aagtgatgga accggttcgt ttctatgagt caatcgcaac
    18721 catgcaggaa gctggcgtga ctaactttat tgaaatcggt ccaggtaaag tcttatcagg
    18781 ttttgtcaag aaaatcgata agaccgcgca tgttgtagca attgaagaca tagaggggct
    18841 acaaatagta ttgaataaat catgaggagg aatcatggaa ttaacaaata aaaatgtttt
    18901 tgtaacaggt tcaagccgag gaattggctt ggccattgcc cataagtttg ccagtcttgg
    18961 tgccaatgtg gtgctgaacg gtcgtggtca gctaggccag gacttgctgg atagctttgc
    19021 agattacggt gtgaaagtcc tagctatttc aggagatatt tcaagcgcgg aagatgccaa
    19081 acgaatggtg gcagaagcca tcgaaacact cggtagcgtc gatattttgg tcaacaatgc
    19141 agggattacc aaggatggaa tggctcttcg tatgacagaa gaggattttg atacggttct
    19201 gaaggtaaat cttactggaa cttttaatat gacgcaggct gttttgaaac caatgacaaa
    19261 ggctcgtgag ggtgctatta tcaatctatc aagcgtatct ggcttgattg gaaatgctgg
    19321 acaggccaac tattctgctt ctaaggcagg tgtgattgga tttactaaag ccattgcccg
    19381 tgaggttgct gggcgaaatg ttcgtgtcaa tgcgattgcg ccgggcttta tccagtctga
    19441 tatgacagat gttttatctg acaagattaa agaagctatg actgcacaaa ttccgatgaa
    19501 acgctttggt gcgacagaag aagtagcgga tgtagcagtg tttcttgcca agcaagaata
    19561 tctaactgga caggtgattg cggttgacgg cggcttgacc atgcggtaat ccagtcttcc
    19621 agtttatctt ttattacttc gacgagtgag gaaactacgt ttccagattt ccagctggga
    19681 gtgggaaaga actcgaccaa gataaaaaga gttcgtcttc ccacccccgc acagttgatt
    19741 aggccagatt tggagtgcaa aacacgaaca aatctgccaa tcaaccactg cgctgagatg
    19801 ttgacacgaa ctctgagtag tggtcctggg ctttttgccc agcctcacga tttccaacct
    19861 tcaatagtcc actggattat tgaagctgtc aacaacttgt tgaaatgaac tttttcgtag
    19921 tactaaaagc aaactgaaag actgcaataa cgcactgtaa attcaatgtt gttttgtgtg
    19981 ttattttaag agaggtttga aaatgacaaa attaaatcgt gtagtagtaa caggctacgg
    20041 tctgacatct ccaatcggaa atacgccaga ggagttctgg gatagtttga aggctgggaa
    20101 aattgggatc ggaaagatta ccaagtttga taccagtgaa tattcggtcc ataatgccgc
    20161 ggaattaaaa gattttcctt ttgacaaata tttcgttaaa aaggatacaa atcgctacga
    20221 taattactcg ctctatgcac tctatgcagc taaagaagcg attgctaatg cacagctgga
    20281 tacagagaca gtggatagtg accgttttgg cgttatctta tcaacaggta tcggtggtat
    20341 cttggaaatt gaagagcaag tggctcgtat gaatgaaaaa ggtccaaaac gcattcgtcc
    20401 catggctctt ccaaaggccc tgccaaatat ggcggctgga aatattgcta tgcaggtcgg
    20461 tgcaaatggc gtctgcaagt gcatcatcac agcctgcgct tcatcaaatg atgctttagg
    20521 ggaagccttc cgtgaaatca agtttggttt ccaagatgtg atgctggctg gcggagcaga
    20581 ggcagccatt actccctttg ctatcggtgg tttccaggct ttgacagcta tgtcgactac
    20641 tgaggatcca gaacgtgcgt ctattccatt tgacaaggac cgcaatggtt ttgtcatggg
    20701 agagggttcc gcggttttag tattggaaag tttggaacac gcagaggcgc gtggagcgac
    20761 gattttggct gaaatcgttg gttatggaaa tacctgtgat gcttaccaca tgacttctcc
    20821 acatccagaa ggtctgggtg ctattaaggc catgaagttg gccatttcag aagcaggttt
    20881 ggagccagct gatattgact acatcaatgc ccatgggact tcgacaccgg ctaatgaaaa
    20941 aggggaaagt caagctatcg tatctgtctt cggcaagaac acgccagttt cttctaccaa
    21001 gtccttcact ggtcacttgt tgggtgcagc gggtgccgtt gaagcggcag ctgtcattga
    21061 ggctatgcgt cattcttacg caccaaagac agctggtacg acagaattat ctgaagatat
    21121 tgaagcggat gtcatttatg gacaggggcg tgatatggaa atccgccatg ccatttcaaa
    21181 tacatttggc tttggtgggc ataattcagt catcgctttc aaacgttggg aggcctaagt
    21241 gaatattaca gaaattaagg acctgatgag tcagtttgac cagtcaagtt tgcgagaatt
    21301 ttcgtatagc aatgctgggg agaccttgca tttcagtaaa aaccagcagg cgacagtggc
    21361 tccgagtcca gcggtagaag ctccgcttgc cccagtaact cctgcaagcc cagcccttgt
    21421 gccagcagtt gaaatgagtg agccagctcc agcagaagca ggtgccagcc ccgtggcaga
    21481 aggtgcggta gtggaaagcc ccttggtcgg cgtggcttat ttgtccccag cccctgacaa
    21541 gccagcattt gtagcggtcg gcgatacggt caaaaaaggc cagaccctca tgattatcga
    21601 agctatgaaa gttatgaacg aagtaccagc tgaccgtgac ggcgtagtta cagaaatttt
    21661 ggtggccaac caagatgttg tagaatttgg acaaggattg gtacgcatca aatgattgat
    21721 attgtaaaaa ttaaagaggc tcttcctcat cgctacccta tgctcttggt cgatcgtgtc
    21781 cttgaagtat cggaagatga gattgtcgcc ctcaaaaatg tgaccattaa cgagcccttc
    21841 ttcaacgggc atttcccaga ctatcctgtt atgccaggtg ttctcatcat ggaggccttg
    21901 gcacaaaccg caggtgtctt ggagttgtct aaggaagaaa acaagggcaa gctggttttt
    21961 tatgccggca tggacaaggt caaatttaag aagcaagttg tacctggtga ccagttgatc
    22021 atgaccgcta catttgtcaa acgccgtgga accattgcgg tcgttgaagc aaaggcagag
    22081 gtggatggaa aactggctgc gtcgggggta ctaacctttg ccattggaaa ataaatcagt
    22141 tctccatttt atcttttatt gcgtcgacga acgaggaaac ttcgtttcct tatttccaac
    22201 ttcaaataat ttcatcatta tttgaactcg ttttgccgta cccaagtact gtctacaaca
    22261 attgatacga attttattcg tctcatttcc aacctccaaa ggtttcccaa acctttggag
    22321 caagcactaa aagtaaaatg aagaactgct attgataacc attatttaat attctcatct
    22381 tgagattgtt ataacaatta ttctatgtta aaaaggagat aggattcatc atgtttgaga
    22441 agattttgat tgccaatcgt ggtgagattg cggtgcggat cattcgtgcg gccagggaat
    22501 tagggattgc gacggtggct gtctattcag aggcggacaa ggaagctctc cacaccatgt
    22561 tggcagatga ggctgtctgt atcggcccgg cccgttcgac agactcctat ctcaatatgc
    22621 aggctgttat ctcggcggct gttgtgacag gtgcccaggc tattcatcct ggctttggat
    22681 ttttgagtga aaactctaaa ttcgccaccc tctgtgaaga ggtcggtatc aagtttatcg
    22741 gaccttctgg gacggtcatg gataccatgg gcgataaaat caatgctcgg gcagagatga
    22801 ttaaggctca agttcctgtc attcctggat cagacggcga agttttgacc agccaagaag
    22861 ctcttgcaat tgctgaaaag attggttacc ctgtcatgct caaagcatcg gcaggaggtg
    22921 gcggtaaggg gattcgtaag gtggaaaaag ctgaagacct agtgcctgcc tttgaatcag
    22981 cttccagcga agccaaggca gcctttggca acggagccat gtatatggag cgtgtcgtct
    23041 atccagcccg ccacatcgaa gtacagattt tggcggacca gcacggtcat gtcattcatc
    23101 tgggagaacg ggattgttcc cttcaacgca acaatcaaaa ggtcttggaa gaagctccgt
    23161 cgattgctat cggccagacc atgcgggaac gtatcggtca agcagcagtg cgggctgccc
    23221 agtcagttgg ttatgaaaat gcggggacca ttgagtttct cttggatgaa gccaagggcg
    23281 aattttactt catggaaatg aacacacggg tgcaggtaga acatcctgtt accgaatttg
    23341 tcactggtgt ggatattgtc aaggaacaaa tcaagattgc ggctggtcag gaactcagtg
    23401 tccgccaaga agatgtgcgg attacaggtc atgccattga atgccgcatc aatgctgaaa
    23461 atccagcctt caattttgcc ccaagtccag gtaagatttc caatctctac ctaccaagcg
    23521 gtggcgttgg tctacgtgta gattcagctg tttacccagg ctacaccatt ccgccttact
    23581 atgattccat gattgccaag gtcatcgtgc atggggaaaa tcgctttgaa gccctgatga
    23641 agatgcaacg ggccctctat gaattggaaa ttgacggagt ggtgaccaat accgacttcc
    23701 agctggactt gatttccgac aagcgagtgg tggctggtga ttatgacact gcctacctca
    23761 tggaagaatt tttaccccgc tatcaagaag atctaaaaaa gtaaactagg agaatgctat
    23821 ggctttgttt cgcaaaaagg acaaatatat tcgcatcaat cccaatcgtt cgcggataga
    23881 gtcagcacct caagcaaagc cagaggtgcc agatgaactc ttttcgaaat gtcctgcttg
    23941 taaggaaatc ctctacaaga aggacttggg accagagaaa acctgtcaac actgttccta
    24001 taattttcgg attacggccc aggaacgctt ggctttgaca gtggatgacg gttcgtttga
    24061 ggaattgttt acagggattg aaaccaaaaa tcccttggac ttccccaatt atttagagaa
    24121 attagcagcc acccgtcaaa aaacaggctt ggatgaagct gttttgacag gaaaggcgac
    24181 aatcggcggt caaccagttg cccttggcat catggattcg cactttatca tggcttctat
    24241 gggcacggtg gtcggtgaga aaattacccg cctctttgag ttggcgattg aagagcgttt
    24301 gccagttgtc ctctttaccg catctggtgg tgctcgcatg caggaaggca ttatgagtct
    24361 gatgcaaatg gccaagattt ctgcagcagt gaaatgccat tccaatgctg gcctctttta
    24421 cctgacggtc ttgacagacc cgacaacagg aggtgttacg gctagttttg ccatggaagg
    24481 cgatattatc ctggctgagc cgcagacctt ggttggtttt gcaggacgac gggttattga
    24541 gtcaactgtg cgtgaaaatc tgcctgatga tttccagaag gctgaatttt tacaagaaca
    24601 cggctttgtc gatgccattg tcaaacgcca ggacttgcca gcgaccattg gtcgcttgtt
    24661 gagaatgcat ggaggtgtca gatgaccagt gatgtagcac gtatgatccg tttggcacgt
    24721 gaccaagctc gcctgacgac cttggactat gccacacagc tctttgatga ctttatcgaa
    24781 ttgcacggcg atcgcaattt ccgtgatgat ggagcaattg tcggtggaat tggcatttta
    24841 gcaggtcagc ctgtgacagt tatcggtatc caaaaaggga aaaatcttca ggataacctc
    24901 aaacgcaact ttggtcagcc ccacccagaa ggctaccgca aggcccttcg cttcatgaaa
    24961 caggcggaga agttcgggcg tcccgttgtc acctttatca atactgctgg agcctatccc
    25021 ggtgtcggtg ccgaggagcg tggacagggt gaggccattg cccgcaacct catggagatg
    25081 agtgacctca aggtgcctat tatcgctatt atcatcggcg agggcggctc tggtggagct
    25141 ctggctctag cggtggcaga tcaggtctgg atgctggaaa actccatgta cgccgtcctc
    25201 agccccgaag ggttcgcgtc tatcctctgg aaagacggca gtcgggccat ggaggcggca
    25261 gaactcatga agattacctc ctatgagttg gagaagctcc aagtggtcga taaagttatt
    25321 ttggaaaatg gcagagcgac caaggaagtc ttggctgaca tcaaggaaaa cctggtcagt
    25381 cagttggctc aactacaagc cttgccactt gataaattgc tcgaaaaccg ctatcaacgc
    25441 tttagaaaat actaggaaga cctagtattt ttttgctaga tttgatacaa tggataaaat
    25501 catttcagga ggttttccat gttagtaaaa gcagatctat caaacgcaga agaattgcta
    25561 cctattcagc accgagcatt tgcggcttta tataaaacct atcaggacca gtacaaccct
    25621 gccattgaaa ctatggacta tttccaatca cgctttgcac gaccaaattg tagctattac
    25681 aagattatcg aggaagagca cacagtcgga ctagtacgga caacggtcgc tgaagacgct
    25741 gaacagggct ggctaggctt gattggtatt gatccagacc acagaggaaa aggctatggt
    25801 cacaaggcta tgctcgaatt ggaaagcctt tatccgacag ttagacgctg ggttctctgc
    25861 acggttttgc aggagtctaa attagtttct ttctacgaaa agctcggcta caaggccatc
    25921 aaaacagagc ctgaacaaga aggcatggat atggtctata tggagaagtg gataggagat
    25981 tcagatgctt gaacagtgtc gcagatacct ggattatcaa ggagttcatt attgcaagcc
    26041 tgaaaaagca ggagctttgg caggcgatat gatagcctta aaaaatgtag cacaggcagc
    26101 acgcaaggag tttcagacat taagtttagt cttagctgag cacgtcagtc cctttgtgcc
    26161 tgagagggtc agtcaatgga tgaatcaagc ccaaatttgc agaccacatt tttggaccta
    26221 ttaccgtttt ccctctgcta gtctaacgga ctcggctctt gctattcgtt tgtatggtaa
    26281 gggaactgaa gcggggattt cagtagaagt cagttttatt gaacgaaaga aagtggagac
    26341 ctctctgctt cagcaaaatc gtgttctgaa ctgctcgatt gcggcaccgc tttattattg
    26401 ggtgcaagag ggacaggtat tttatcgcct ggatggaacg gaggagaata gacagagcct
    26461 attacaagcc gttgaagcag gacggattcg taaagtattg gtcaagtatg atgtaccgtt
    26521 tagaaccgtt gcctccgagg aggaactgct ggaacgcttg attcagggct tttctctact
    26581 agagccctac ttgaagatga cacacctcgc taaataatca gtggggtgat ggcaacaaac
    26641 atcgtaaggg tatttgtttt ttagtgtata ttgacaaatt cattgaattt ttgtaaaact
    26701 gtatcgcaac aacagaacag tttgattgac attacgtgaa atcctataat agattgaagg
    26761 cactctatat gatgaagcgg aagaggagaa gagtatggaa aaactaggtc aaagtttgaa
    26821 atggcttggt cggaagattt ggctattatt aaggattttt ggtctcttag tgcttgctgt
    26881 ccttccagat ctgattttgg aagggtccaa atcgcaagtc accttggtaa tagttggact
    26941 ggtgacactg ggtcttctgg tctttttctg gtggcgggct gagaaaaata atctaccagt
    27001 ctgggacaaa aacgttcttt cttgggacgg tctggcaata gtcatactag cctttgctac
    27061 catgcaactg acagacatgc ttggtttcta tttgttggaa ttgcaggggg ctacaacgac
    27121 agctaacgac gcctccatca tcgaatcttt gaagggagtt ccttttgggc tggcactctt
    27181 gacaacagca tttttgccag caattgcaga agaggttata ttgcggggtt atttctttaa
    27241 gaaattgttt gggtcgcaag cagttgttgg aatcattgtt tcaagcctgc ttttcggagc
    27301 ccttcatgga ccgaccgatc ttgcctcttg gctgatttat ggaggtggag gtcttatctt
    27361 ttgtgttctc tatcataaga cgggctactt gatctatccc atcgctgtcc attttatcaa
    27421 caatgcctgg tcggtagtgg cactctacta ttttcaataa gcaacagaaa agacgattcc
    27481 gagtggaatc gtcttttgca atgaccagta caagctttat cacgatagag ataggaatgt
    27541 caacaaagtc ctgaaagtga gcgcataatt aaatcaacat cttggttttc caactcacga
    27601 atgatatgaa gataggtttt ctgggtcgtt gttatgcttg catgaccaag tcgtctagca
    27661 acacttgcga tagagacccc ggcaaataat aaaatagatg catgggtgtg tcgcagggcg
    27721 tgaatggtga cgacaggaag atttgcactt aggcaccgct tttcaagaat ggcattgatg
    27781 gtagagttgt aaatacgttc tttcttcacg aaaagtaggt catcaggttg ggtgtgctgc
    27841 aataagttgg cgaattgggt gctggtttgc cagtcgagtt ggatttttcg aattgaagat
    27901 ttattttttg taggtaaaaa tccgccgtct cctttataat tccatgtttt atcaatggtc
    27961 aatagctgtc ggtgaaaatc aaaatcttga gtagtgagag caagggcttc ggagaatcga
    28021 atacctgttt tagcaatcaa taagatgagc caatcccaat tcagctcctc tccgagacgt
    28081 aattcttgta tcaactgatg aagttcaaat tggttgaggt attttgtctt tttctttcga
    28141 ggaagtcttc ctttgataat caccttacgt gtcggatctc gttcaataat tccttcatct
    28201 acagcatcta aaatagctcc tttcaactgg tgatgaaaat ccatggttgt ctgtcgttca
    28261 tgtgttttag catagctgtt aatcaattct tggtaacgaa cccgattcaa atcttccaac
    28321 aacagactag gagctagcct gataagccac tgcaatgtca ttctatattt cttgagagtg
    28381 acttctcgaa tggctccttc cttgtaaatc cgaagccaat ttgcataata gtgatggaaa
    28441 agtagttttt cttttgataa gttcattgta gaacctcctt ttttaatttt tgttaattac
    28501 ataatttatt gacagatttc cgaggagatg atatacttat tttagcgaaa aaatgtaatt
    28561 atgaaaaatt tcaaagaaag ggaaattatg aaactagaag aattggctct ctttacggga
    28621 ggtagtttac aggttcgctt ggatactacc agctgggaag atgctagaga ctatatcctc
    28681 tacaatcagc aacatcatca attggatggt tatgaggtga tagaagaagt gatagatagt
    28741 cggaccgtga cgacagatag agaagtgacc ttgctagagg agggagactt actctttagt
    28801 ttactatcgg ggaaggctgt tttagtgaga gcagagcacg caggcttact ctatacacag
    28861 aactatatca aaattgagcc cattgcaaag ctggataagg ccttcttact gtatctgatc
    28921 aatgaatcct cagctattag acggcagttt taccaaagct tacaggggag tgaggtaatg
    28981 aaatatacag tcaagcaact gaaaagttta cagctaggcg ccttgcctcc tttagatagt
    29041 cagcagaaac taggaaagat ttatttagat ggattagcct tacggcagaa gcggcaagct
    29101 tatgcgcaag gagatttttt acgactaaca tatttgttaa aggaggttta cgtacatgaa
    29161 tgaattagca tttgaaacag aattgattca atatattacg actgggacta ttacacagcc
    29221 ggagcaccta gaaggtctag aatggtctgt tagtgattct tcgaccctgt atgcagtcaa
    29281 atctaaactg tggacttatg agccgggtat caagacaacg gagcaattat gggaaaattt
    29341 tcgtaatatt ctctggcaac acaaccaaca tcgattggac aaacccttga gcgataccga
    29401 gtttgctcag gtcaagcaga ttatttcaga tctgaagaca ccttatgagg ctggtcagtt
    29461 tttatatgga attagcggtg tttccgaatt ggaaattaca cttgatgatg gacggcatac
    29521 atttttgaca gtattcgacc aacgtaagat aggcggtggg gacactgtct accaagtggt
    29581 gaaccaaatc cgtaggactc ctaaaatccc tggacgacga gaatgtatat tcgatacaac
    29641 cttgttgatt aatggcttac ctattattca gattgaagag aaaaaggata cggtagatgt
    29701 ggacaaggca ttgaaccaaa tgcaccaata tatccaggaa aggcaatata cggatatttt
    29761 ttctaccttg cagattctag tcgctatcgc ccccaacaat gttcgctata tggccaatac
    29821 cactgcggaa aaatttaata aggactttgc tttccactgg cagcgtaaat ctgacggtag
    29881 taaggttcgg aattggaaag aatttgcgga ttccatgctc agcattccta tggcccatca
    29941 gatggcgacc aactacatga tcttagacgg gacaccgcag aaacaaatgc tgaaagtcat
    30001 gcgtccctat caagtttacg ccactcaggc ggtcattgag gggataaaaa aagtcgattt
    30061 tgaactaggc tgtaacaagg ttggctacat ctggcatacg acaggctcag ggaagacgat
    30121 cactagtttt aaaacagctt ggctggcaag tcgaatgccc aaggtagaca aggttgtctt
    30181 tctagtggat cgtatcgcat tgaccaatca gactaaggat gattatctgg cttatgatcc
    30241 agaagctggg gagaatagtt ttggcagtat tcgagaaact caaaatacca cagccttggc
    30301 acggcagttg actagtaagg atagtgggat tatcgttact tctgtgcaaa aattggatac
    30361 actggtgaag agaaaatcct tcaaatcacc agataagcat atcgtgttta tcgttgacga
    30421 agctcatcgc tcaactggtg gagatagttt tgcagggatt caaaaggcct ttaagaaagc
    30481 ggcttggatt ggctatacag gaactcccaa cttagatgat acaggtgcta gtctgcggac
    30541 gcaagatatt tttggccctc ttcttcatgc ctacactatt aaaaatgcca ttagtgaccg
    30601 caatgtacta gggtttaagg tagattttga gacaaccatc gatcaggaaa aactaaaaac
    30661 aacttactta ccagattttt atcggcaaaa atacccgaag tggacaagtg cacagattga
    30721 agaaaaaatt gccaatctaa gtccagatga tatggatgat gcggtgactt ctagcgtcta
    30781 tgatgaaaat ccagagcatg ttcgattggt agtcgaagat gtcttcaaac actgggcaaa
    30841 tcgctccggc agtggccatt acaatgcctt gttcaccacc cacgtaggtg gaggcagggc
    30901 ctccacaccc atggctatga tgtatttccg agagtttcaa cgaatcaatc aactgcatcg
    30961 agagcaagga ggtctggtct tgaaggtggg agtgaccttt gccttgaata ctcaaaataa
    31021 tgatagcatg gtcgagtcaa atactggttt gcttgaagct attacgatct ataacacaga
    31081 atttgggacg tcctttggct tgtcagatgt tgcttcttat acagaggacc tggttagtcg
    31141 cctcaataga acagccaagg acaagaacta cttagatttg gtgattgtcg tggatcagtt
    31201 gttgacaggt ttcaatgctc ctgaactcaa tactctttat gttgaccgaa ccttaaaagg
    31261 agccagtctt attcaggcct attctcgtac caatcgtgtg gaggatatgg ataaaaaacc
    31321 ttttggacgg gtggtcaatt accgctggcc agcccataat gagaaattga tgaatcaggc
    31381 cttggccatc tatgcagatg atagtgctgc caatttatca gaagcggaac aaaaaaataa
    31441 caatgaaaaa aatggactgg tggctaagcc ttttaaggaa gtatttgcgg aagtcaaaca
    31501 aacggtcgac caattgagag agttgacggg tgatttcaat gatattccga agtctgagaa
    31561 aaagcgtgag gctatgttag aattgctccg tgcttacaat caaggactgt ctaagctcaa
    31621 acagtatcag ccagaggagg ttgatggggt gattgaagga tttgattaca atcagccgga
    31681 ccgctttatt gaaagtttgg gaatgtctgt agaggaagaa aaacttttga cgacagtctt
    31741 agccaatgaa ttgaaacgaa ccttggcgaa agagcaaaaa gttagcattc accaaattga
    31801 actcaagatg actcacatca aggaagtgac aattgactac gataaattga ccgaactggt
    31861 tgagctcttg ctgaatcagg ttcacgaaga gaagatggta gaagccgcag ctaccaggga
    31921 tgaattggat agatttgcaa gcggtttgga agacctcaac tatgctgacc aaatccgtca
    31981 aacaggggat gcaatctatc gaggtgattt tccgacacaa gatatgaact ttgcttatcc
    32041 agtcaagggg cttgatagtc agaagattat ccaagaagct tcgcgtgttt atattgacaa
    32101 gaagctcctt gcttttcgaa tccaatgggg cttgacggag catatccgca accaagaact
    32161 acgagagttg ttggcgagac atcgatatgg tcagaatgac ttggatgata ccggacactt
    32221 acgtaccttg ataaaagaag ctagtcgcga ttaccagacc tgggcagaag gtgatgctgt
    32281 taaaggcttg agcaagattc agtaccgcaa tcagttgaga caagcactat atgatttggc
    32341 tgacgacttg gttggtcaat aggttgcgat tttgttggat gatagcgtat aatagaatgg
    32401 aaatcgtccg aaaaattgca aaaataactt tcagaaatag aacaaaaaga gagtagaaaa
    32461 ggagggacta tgacaaaaga aaaatcaacc gtaccacgat tgcgcttccc cggattcacg
    32521 gacgcttgga aacagcgtaa gttgggggag gtggcggact tttccataaa aacaaactca
    32581 ctttctagag ataagttatc atcgtatttt tatgaagttc agaacattca ttatggggat
    32641 attttaacaa aatatgatgc tattttagat gtatgtaaca aagaacttcc atcaattatt
    32701 ggaagtacga tttcagactt tgcagatgct ttacttagtg aaggagatat tgttttcgcg
    32761 gatgcagcag aagactcgac tgttgggaaa gctattgaag ttcgaaattt taagggtaag
    32821 aatgttgttt ctggtttaca tacgatagtt gctaggccga aagtttctta tgccccctac
    32881 tatttaggtt atctaattaa ttcaactgca taccataatc aaattttacc tttaatgcag
    32941 gggacaaaag tgagctcaat tagtaaggct aatttaaaat ccacgacagt agtgttcccc
    33001 accctccctg aacaagaagc catcggcagc ttcttctccg acctagatca gcttattact
    33061 cttcatcagc gaaaattaga tgatgttaaa gaattgaaga aggctttgtt acagaaaatg
    33121 tttccgaaag ggaatggaaa cgattttcct gagctaagat tccccggatt cacggac
//
LOCUS       DAZQCJ010000011        32778 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000011 DAZQCJ010000000
VERSION     DAZQCJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32778
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.011"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(113..4525)
                     /gene="essC"
                     /locus_tag="ACHU66_000486"
     CDS             complement(113..4525)
                     /gene="essC"
                     /locus_tag="ACHU66_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HFR3388796.1"
                     /translation="MAETVVFYKDGFRYAADVSAGKKILFQGTEKAQVFVSSLDTPLT
                     IWLDGVQVCYDYDGVEGNLVSGMSLAGGVVYLLPSEQVILDPLDKQELTIGQHVGNSF
                     VLAGSSCHVLLKRSDQSWMLYRLAGSIYINNLLMEKGEMELALGDELAFEDTFFKFYA
                     DEVLVAGPVEASDELARKSASRYAFYEDYPDYHRSPRIIYRSSEDRVAINAPSNAPSK
                     PSDSLLKLILPPLMMVGITLVIMIFQPRGLYVLATIAMSIVTLGMSIAGYIKGRKDYQ
                     KELRDREGLYHDYLADKAKELAGLTKSQKDGQLYHYPAIETLVDLADSYHHRIYEKTP
                     LHFDFLYYRLGLGEVPVSYDLSYAQTERSGKRDPLELEGFQLYEQNKTISDMPIVANL
                     SHGPVGYIGPRALVIEQLQLMVNQIALFHSYHDVQFITIMPEEEKEQWDWMRFLPHAT
                     LQDMNVRGFVYNQRTHDQVLNSLNQILKLRRAQKEDKSNRESTLFSPHYVVLVTDEKL
                     ILDHVIMEFFTEDPTDLGCSLVFVQDVLSSLSENIKTIINIKDRNTGQLVMEEGQLRE
                     IDFALDHFPVGYDKETLVRRLAPLNHLQNLKSSIPETVTFMEMYGAETFEDLGVVSRW
                     EKHAPYKSLAVPLGLRGKEDIVYLNLHEKAHGPHGLVAGTTGSGKSEVIQSYILSLAV
                     NFHPHDVAFLLIDYKGGGMANLFKDLPHLLGTITNLDGAQSMRALVSINAELKRRQRL
                     FATHDVNHINQYQKKYKLGEVSEPMPHLFLISDEFAELKTNQPDFMKELVSTARIGRS
                     LGIHLILATQKPSGVVDDQIWSNSRFKLALKVADRSDSMEMLKTPDAAEITQVGRGYL
                     QVGNNEVYELFQSAWSGADYQPDKDEQGIEDHTIYAINDIGQYEILNEDLSGLDQAES
                     IKEVPSELQAIVSKLKALTERLSIQALPQPWLPPLPSRLFVEDLRDEAELTWSGVNEE
                     LEVILGLLDKPSQQAQEPLSINLSQDGHLLMYGAPATGKTTFLQTTVLDLARHYSPSE
                     LHIYLMDFGTSGLVPMGDLPHVADVFLLDQGEKIGKFIRLLEKEINRRKKLLTEHRVG
                     TLSMYRQLTGQVEPRIVIALDSFESMKDESFEAELLKLLTRISREGQAIGLHLVVTAS
                     RQANLRATFYSNFKHQLTLKQHDKTEVRSVVGATGLADIEDIKGRALYKGEEVYSLQL
                     ALPLRVEHDIELVDQMREEVSRQKAAWTGQVPEAIPMVPEELTREDFLSRASVQKAIA
                     SGQIPLGLDLETVEAVSWDLTVGNFVYIYEQERHRNNFLRGFLNTLGQTDYSVKMVSG
                     EEFFTDNGTEVSFTPSSRLLMIDEIYDSVFENIEFDEYDEIVVVIWNELDVLVQEDSQ
                     LLDKMLYILTNGPKVGVYSLIVTTPGLSSKIDVVSKHVKLMKEAMVETRLNDQKILSL
                     NNVKYSEPSLQESLAYFIANKNYKLMKTVSEGSK"
     gene            complement(4529..5791)
                     /gene="essB"
                     /locus_tag="ACHU66_000487"
     CDS             complement(4529..5791)
                     /gene="essB"
                     /locus_tag="ACHU66_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HFR3388797.1"
                     /translation="MKEETFVFGGQEFVFLKGDKEWHLDVKRSDVATQELSDLLLLDL
                     HHPQFLQQEMTVDEDTVHFTYHLDAFGQTFEDMKEKEVSEKLRLSLNVLELKQALKLP
                     ITFFVHPSSVFITKDGRAKIAYRAIPEKMVPQTLDATDLLKQLKSYILALFTEHDFMD
                     LYNGALDVVPVPEFLDTIRKAEDMDSLEGLLQGYFQDKLEEEKATLTRVSRSKHRLYR
                     YATIWLSALAGLLVLPLIYLVFMHNPFKEKMLDADTAFIKVDYPGVISELERVEVSSL
                     PYTQKYTLAYAYIQGFDLSNEQRSVILNNVTLKSDEQYLDYWIEIGRGNSQEALDLAK
                     RLDDSDLILYALYQQMEQVRENTTLSGEERETTLDELKAEYQKYWDTRTTILNEDGSQ
                     VKTGTSSTSSSTSSTESSSTSPSSSSGE"
     gene            complement(5793..6032)
                     /locus_tag="ACHU66_000488"
     CDS             complement(5793..6032)
                     /locus_tag="ACHU66_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HFR3388798.1"
                     /translation="METHINVTLHDGGRIRDLRIPTHIEVRRLIKELDSIFEKENARK
                     KYQLHVVNKGFILDEGKVLADYPVTTGDIVEVLEV"
     gene            complement(6062..6526)
                     /gene="essA"
                     /locus_tag="ACHU66_000489"
     CDS             complement(6062..6526)
                     /gene="essA"
                     /locus_tag="ACHU66_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HFR3388799.1"
                     /translation="MKKIALVLSCLVLFLPVGIEASELGDNSLQIDSSRIEVEKEKKI
                     GSQDLLVQSLFLAPDQAELTAKKEADQVLLEHQLNSLFQTGEKPSTDILQVGTLFDTR
                     THKVMTTKVDSGTEGAPAGLLSGLFFASIVVGILLLASLATYLMGKDEVGQE"
     gene            complement(6527..9676)
                     /gene="esaA"
                     /locus_tag="ACHU66_000490"
     CDS             complement(6527..9676)
                     /gene="esaA"
                     /locus_tag="ACHU66_000490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HFR3388800.1"
                     /translation="MDKQRIFKYGRNALLVLLLLLSIIGLNIAVQNNTSISKKEQEAG
                     QQNIKLDVALVNEDNTVTLNGTEYNLGASYAKNIEKNTAHNWSTVSRGTADKGLKEGH
                     YQLLVTIPSDFSTKILDINNTNVDKATITYKINANGNLQVENEAVKVAKGIIADLQSQ
                     LIDMYMASILGNLYQAQRNVQTLSDTKVTNIAVFRNNLYQTAVDFKTLFPNLTSTADS
                     TLAANNLLKEALSSNVTAYDSLNTAQTGLKDNLATLIEQRNQGKLSQEEFSASLLAMN
                     SSVLSAETDQLFATIKTTQEVLSGQLGAAAIDETGEASGYIGLVEKLDQAIADLQVAI
                     EAESTKLAEHEATISNFAETHLTTYYGDSLETIHIGAVLGKSTELPISLASYQTSLDQ
                     LILNQLAGLPSLTPADLTTDLNKLDPKAASQIRFDAAFAQAHFGEHFVASNDLKELQA
                     LAKELEPHLQAAADFKIPTPTTRATLSLEIPSGFDLVSWELNGNSYTNPSEVVALQKD
                     NTVTVTLQYEKSQTTTVSTTVVTDSTSTSSTTGSSSSEENTASTTAPSTGTPVTTTEM
                     AQGSVKVSVNNLEVTTAGFDWENFLKEQEAYRKAEVAYAAKVSEIISKYNQANRLLDQ
                     YYPVNTVTGERSSLTDSFFNQDAKSLLTSLVTSSITANLQSYSEARAQDQALKERLAE
                     LEGRKQELKDQLQYISTTNSDLAQQIANQLAFLEDVKARAQSIADGQEQQTALQGTHD
                     TELAALATELDASLSITGEVKSLSEQNVEEATNVNTVFTTFTNDVQAAQESGENLSSE
                     ATALMERFESELAENGDFVKTFTSVLSNTYDNGVPNEVILDFLSAPLTESSSSVKATV
                     NTYRPFTWILLLEMVALFAAYVFGTHRVIKAVKDKFKVSRLEDTDLVTVTVLSVLSLL
                     VGGLIGVVSSQQLQIDSELIPTWVLMITLFSLLLAQGQYLLIKNAKGLGMGISFFMVI
                     SFIYLSNAIGTTASLTGLPATLKAINPLSVLENSLSGYFDGEATSLTVIATVLVLGLG
                     FAALTAFVDVEKFLNKKED"
     gene            complement(9750..10040)
                     /locus_tag="ACHU66_000491"
     CDS             complement(9750..10040)
                     /locus_tag="ACHU66_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HFR3388801.1"
                     /translation="MSRIKLTPDELRTSAIRYTEGSDNIDAILSSLSNEQSVIRENWE
                     GTAFDSFDAQFEALKPKIVEFSELLRDINAQLNKVADIIEQTDADIAAQING"
     gene            complement(10324..11136)
                     /gene="purR"
                     /locus_tag="ACHU66_000492"
     CDS             complement(10324..11136)
                     /gene="purR"
                     /locus_tag="ACHU66_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFR3388802.1"
                     /translation="MKLRRSERMVVISNYLINHPYELTSLNTFAEKYESAKSSISEDI
                     AIIKKAFEESSIGHIETITGASGGVVFTPSISKAESVEIAQALRDQMAESNRILPGGY
                     IYSSDLLSTPHILKNVGRIIADAFKDEKIDAVMTVATKGVPLANAVANVLNVPFVIVR
                     RDLKITEGSTVSVNYVSGSSDRIEKMFLSKRSLKAGSRVLIVDDFLKNGGTINGMISL
                     LSEFDSTLVGVAVFAENQKGDRSVANYKSLLAVTDINVKENRVDVELGNIFE"
     gene            complement(11360..12097)
                     /locus_tag="ACHU66_000493"
     CDS             complement(11360..12097)
                     /locus_tag="ACHU66_000493"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HFR3388803.1"
                     /translation="MAVRIVSRQPLTKGWSTDRKYKVRLEDGRLGLLRIAERPAYEAK
                     RLEFQLVKNLFGLGLPVAEPIALWVGEESVYTLYEWVEGQDMNEVATSLSDSVLYDLG
                     CQSGQFLRTLHALPIDQSQRDWNSFYQAKIDNKLAVYQAASHSYPNGQAMIDFVQANR
                     HLLEGRPYHHGDFHTGNFLLGADGKLKILDFDRYDIGDPWEEFNRLIFTADLSPAFAR
                     GQVDSYFDGAIPEEFWRLMALYVTVIV"
     gene            complement(12097..13038)
                     /locus_tag="ACHU66_000494"
     CDS             complement(12097..13038)
                     /locus_tag="ACHU66_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HFR3388804.1"
                     /translation="MKINQMKKDEFFEGFYLIKTAEVRQTRAGKDYLALTFQDDTGEI
                     EGKVWDAQPGKIKDFTAGTVVHMQGRREVYNNTPQVNQLVLRLPKAGEPNDPADFKEK
                     PPVDVKDTKDYLSQMIFRIENATWQRIVRALYSKYDKEFYSYPAAKTNHHAFYSGLSF
                     HTATMVRLADKIGDIYLQLNKSLLFAGIMLHDLAKVIELTGPDNTGYTVRGNLIGHIS
                     LIDEEITKVLMELGIDDSLEEVTVLRHVILSHHGLLEYGSPVRPQIMEAEILHMIDNI
                     DAEMMMMLSALDKVGPGEMTNRIFAMDNRAFYKPNID"
     gene            complement(13028..14266)
                     /locus_tag="ACHU66_000495"
     CDS             complement(13028..14266)
                     /locus_tag="ACHU66_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR3388805.1"
                     /translation="MDIVLLILLILVLLALVFLYGKWQSLALLLQDQAEDTADNLSDQ
                     LSYQLENATLKQQQAIHQEVERLRTELYQQLTDIRQELNRSHLETRDATDRRLQAIQE
                     SNEKRLKEMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVARDVGSLNK
                     VLSGTKTRGIMGELQLGQIIEDILTPSQYEREFATVSGSSERVEYAVKLPGRTEGDYI
                     YLPIDSKFPLADYYRLEDAYESGDKDQIDLHRKNLLAAIKRFAKDIQSKYLNPPETTN
                     FGVLFLPTEGLYSEVVRNPIFFDELRRQENIVVAGPTTLSALLNSLSVGFKTLNIQRS
                     ADDISKVLGNVKLEFGKFSDLLLKAQKQLNQASSNIDKLLTTRTNAIERSLRTIDLYE
                     DDQTKGLLGLSPLDEEDNEN"
     gene            complement(14267..14899)
                     /locus_tag="ACHU66_000496"
     CDS             complement(14267..14899)
                     /locus_tag="ACHU66_000496"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFR3388806.1"
                     /translation="MIKIAVIAGGSFDCFPEPADLYVGVDAGSLRLLDHFLPLDWAIG
                     DFDSVTSEELGRIKDQAERFLQAPAEKDDTDLELALKEVFKVYPQAQVRIYGALGGRM
                     DHMMANLFLVAEPDLAPYMEQIELVDSQNIVRFRPAGQHRLSPIAGMKYISFMPSDQS
                     RLTIRHAKYPLDASNYFFKKCYASNEFIDRDIDIQLDQGYVVLIYSKDKN"
     gene            complement(14892..15551)
                     /gene="rpe"
                     /locus_tag="ACHU66_000497"
     CDS             complement(14892..15551)
                     /gene="rpe"
                     /locus_tag="ACHU66_000497"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR3388807.1"
                     /translation="MSHYKIAPSILAADYANFEKELKRLEKTGVEYVHIDVMDGHFVP
                     NISFGADVVAAMRPHSKLVFDCHLMVSNPENHIETFARAGADILTIHAEATVHLHGTL
                     QKIRAAGMKVGVVINPGTPLVTIEPVLNLVDQVLLMTVNPGFGGQAYIPEVAEKIADL
                     VKLREAKGLNFDIEVDGGIDDKTIHSAKNAGANVFVAGSYLFKGDLDANVAKLRAALH
                     D"
     gene            complement(15566..16441)
                     /gene="rsgA"
                     /locus_tag="ACHU66_000498"
     CDS             complement(15566..16441)
                     /gene="rsgA"
                     /locus_tag="ACHU66_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFR3388808.1"
                     /translation="MQGRIIKALAGFYYVEADGQIYQTRARGNFRKKGQTPYVGDFVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSAKEPDFNANLLDRFLVLLEQK
                     DMDPIIYISKMDLVEDRTEMDAFKAIYEKISYPFVYSLEELTPLLQDKVTVFMGQTGV
                     GKSTLLNRIAPELALETGAISDSLGRGRHTTRAVSFYNVFGGKIADTPGFSSLDYEVK
                     EAEALTDCFPEIAESSQDCKFRTCTHTHEPDCAVKEAVASSAISQSRFDNYLQFLSEI
                     QNQRETYTKVSKKFK"
     gene            16566..16647
                     /locus_tag="ACHU66_000499"
     tRNA            16566..16647
                     /locus_tag="ACHU66_000499"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16599..16601,aa:Leu,seq:cag)
     gene            complement(16921..18498)
                     /locus_tag="ACHU66_000500"
     CDS             complement(16921..18498)
                     /locus_tag="ACHU66_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR3388809.1"
                     /translation="MKKRNFWLTLTCIFAVALLNVGFLYQYRYIIDAIAAGDRQAFVS
                     YFVLMGLTVLVMLVFEYIRQIANVSYLNQVGFQLQATFIGKIFDLPFRQFVEQGAGAY
                     ISQLNNDLETVKEDYYDAFFTIFQGACTFGIASLALLSLDGLTAIFLILISFLPVVIP
                     YLFKKRRRLNQEAISDSQQVYNTRVSDVFLSYLQVKNSHQRQQVLDGLNDRYQAVNDK
                     VNQATRTTVTMRLLVGFVFYLTTLSIIFIGGFQVFSGALTIGGLTAILTISEQLVDPI
                     NSIAAAFLDRHAVKKLKQEFELSTFGQETKGQELASAFQSIQLVKASYAVGEKQVFED
                     LTVAFERGKKYLILGESGSGKSSLALILTKNSQLQAGDIYVDKTSLSDLSYQAIQDKI
                     AYLPQEGSLFHDTVLYNLTMGREIPEDRLMFLIKTMNLDSRFPSYASLSEEISDDSGL
                     SGGQKQRLLLIRALLQDKDILLLDESLSALDQETYAVIENYLTSLADKTLIHISHRMS
                     EEVLSRYDGVVRIGESN"
     gene            complement(18788..21232)
                     /locus_tag="ACHU66_000501"
     CDS             complement(18788..21232)
                     /locus_tag="ACHU66_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFR3388810.1"
                     /translation="MKQIKLKATKQFILVMVMTVLLAMGRALLLSDVSFPLWFIPLPL
                     IAYLIFTFYVLVLLDKYEKFIKTKTFAKYVSYFLGFLYLVNLVYRLNAKKYQPWNIFR
                     NNLFQFELLLMLALPVLLAFLWRKKTGIREKLSQWSANHLVPDGYLLLTSLLSLSPLA
                     ISYWKDSHYESLVEKGSYIEFFSQTPLFAPIHFIGTYIFLRYLHKAFVEFKANRTNVH
                     SMLFISLALATLSHIGYQASMAGATGSYFTRHLFPGAIVFQIACLFFLNVIISLVINR
                     QILSVAVIASLNVILVTANFLKFRYRSEPLTPNDFKWVGNLGMILSFISLRVILVSLV
                     FIVLFVFIYRHIHKKYFRGQIVAAIWKRLAGISVIVSLILGMGWAIRNEKDHKIAGWI
                     PILSQVNNWRNVDWKGYAFVARYRTLSFLWLQQLSKTSMEMPENYSEKTMKAIVKKYT
                     ALAEEINAERTGQLTDQTVIYILSESLADPRRIPGVTLSQNVLPNIEYIMSQTTSGLM
                     KSDHYGGGTANIEFQVYSGLPFYNYSSSISSVYLDVAPNMKKLPSISDLYPAENRVAI
                     HPYFDTSYNRNSIYKQLGIEQFYTLNSAKYPLAVTAEDYQGNFVSDKKTYDLILEQVR
                     SGTNTFVSAITMQNHVQWNSAEPASITASGEGFTAEENANLNSYVRLLSFTDQATRDF
                     LDQLKTLDKKVTVVFYGDHLPGLYPESAFVTDPSAQYKTDYFVWSNFETEDYHYDLVN
                     SSDMNALMLETTNNKVSPYYALLTEVLHKDRVGQAERDAKVAEELKLVQYDLSTGKGY
                     LLKYKDFFKVATETTE"
     gene            complement(21245..22975)
                     /locus_tag="ACHU66_000502"
     CDS             complement(21245..22975)
                     /locus_tag="ACHU66_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388811.1"
                     /translation="MLKKLLKKHPDIVAVTVFFLFSLTLLLPQILSKAVVTGDDIVFH
                     YNRFYDTAMQIKSGDFSYIMSLHGYQQSGRIVNALYGPYLAYLQGALLLLAGTWYRYQ
                     LLSNLLLGVLSATSLYFLMREVKVRYSLSVGLSMFFVTTYSVQYWWVAQGFSSWGVAL
                     FPLCLIPAVRFLKTGKVPIFLMAGAVGLMLQIHMLSALFLILAYAILFSIGWLKSQDK
                     WNVMKDVLFSVGVFLILTVNIWLPLLYVNATNELAAPFINKKFIQSTVTWSKAYFLYF
                     PYSLPIIFATFLYFMLKKWKKQSVILRGLTVTFLVFFIFSTNLFPWQLFAGKGIGLVD
                     LIQFPFRFFLYANALLLTIVGLQLTDYEVRKKLFLGLTILSTIIGVGFTWDQYNQAVN
                     DQYNKAEFLQDMLHTTLYGTTEELRASLHSKNLGEFVQLAQKSTPDYVPVLSEKEENV
                     RAVNEAKSRYDRYRDQIILPNQQFNKQVFGDSLKISWSANQSEEIQIPIVIYRDSELT
                     LNGKRLGKMDYYLSTIGVPTVTSQKGKNTLILSYKQQWWLLPVIFISLTGWILWSIYY
                     TFIYRKSNIS"
     gene            complement(23338..24210)
                     /gene="rsmA"
                     /locus_tag="ACHU66_000503"
     CDS             complement(23338..24210)
                     /gene="rsmA"
                     /locus_tag="ACHU66_000503"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR3388812.1"
                     /translation="MRIADKNVTRAVLERHGFTFKKSFGQNFLTDTNILQKIVNTAEI
                     DQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLIPILADTLGRFDNVTVVNQDIL
                     KTDLQTQIQNFKNPDLPIKVVANLPYYITTPILMHLIESKIPFSEFVVMMQREVADRI
                     SAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRREQPLVSVKD
                     EDFFFRVSKASFVHRRKTLWNNLTNHFGKSDEVKDKLTRALGMAELEASVRGEALSMA
                     DFARLSDSLQEVGL"
     gene            complement(24230..24760)
                     /locus_tag="ACHU66_000504"
     CDS             complement(24230..24760)
                     /locus_tag="ACHU66_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HFR3388813.1"
                     /translation="MKKVVLLRGVTPVGKNKIPKMSYLAEILTEAGLTSVQTYIQSGN
                     VVCETDILDEELSRLIHQTIKEKIGAELAIIVKHQMELTQAVAENSFGEGYDPSRVHL
                     VFTNDEINGEKLNKLLHQDFGDEELRLGSQCLYMYLPRDARKKKLNTNYLEKQLDITA
                     TMRKLSVISRLSEMAK"
     gene            complement(24774..25031)
                     /locus_tag="ACHU66_000505"
     CDS             complement(24774..25031)
                     /locus_tag="ACHU66_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFR3388814.1"
                     /translation="MGIHFTDDAWEDYLYWQGQDKKTLKRINKIIKDMQRHPFEGIAK
                     PEPLKYDYQGAWSRRIDAENRLIYMVEADRLCILSLKDHYR"
     gene            complement(25033..25293)
                     /locus_tag="ACHU66_000506"
     CDS             complement(25033..25293)
                     /locus_tag="ACHU66_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFR3388815.1"
                     /translation="MEAIVYSHFRNHLKDYMKKVNDEFEPLVVVNKNPEEDIVVLSKS
                     EWDSLQETLAVARNTYLSQKVLRGMAKVKTGQTQERNLIEAD"
     gene            complement(25369..25824)
                     /locus_tag="ACHU66_000507"
     CDS             complement(25369..25824)
                     /locus_tag="ACHU66_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HFR3388816.1"
                     /translation="MSRREQVVLTNMCMITDGRRVLVQDRKSEKWPGVTFPGGHVEHG
                     ESIVSSVVREVKEETGLTVSNLELCGIQNWTDPTDHYRYLVFCYKTSHFSGSIQSSDE
                     GEVFWSDRAELKNVQLADGFETMLEIFEQPQLTENFYWFEDGEWKVENR"
     gene            complement(25828..26196)
                     /locus_tag="ACHU66_000508"
     CDS             complement(25828..26196)
                     /locus_tag="ACHU66_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388817.1"
                     /translation="MDRVYAVKCLVKNEFEPTYENYSSYEEVTYLLPANVLVWDDEQV
                     DKNKLADYLRGLVEDGYVNADGIWKQSRLFHIAGVQEMDDRVEFDGPAVEVYRWFYNF
                     ERPVTEEEFLNLYYFDDLGR"
     gene            complement(26189..26758)
                     /gene="rnmV"
                     /locus_tag="ACHU66_000509"
     CDS             complement(26189..26758)
                     /gene="rnmV"
                     /locus_tag="ACHU66_000509"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFR3388818.1"
                     /translation="MTQKIKIQEVLVVEGKDDTANLKRFYEVDTYETRGSAISDDDLE
                     RIEKLHDLRGVIVFTDPDYNGERIRKIIMQAIPTVKHAFLQRDEATPKSKTTGRSLGI
                     EHASFEDLEKALAGVRGYYDDENNFDISKSDLMRLGLLMGGESRKRREYLGEKLRIGY
                     CNGKQLLKRLELFGISLVEVEEAMESYHG"
     gene            complement(26742..27524)
                     /locus_tag="ACHU66_000510"
     CDS             complement(26742..27524)
                     /locus_tag="ACHU66_000510"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFR3388819.1"
                     /translation="MIQIFDTHTHLNVEQFDGRVQEELDFAKEMGVTLHNVVGFDRAT
                     IDKAMELADQHPEIYLTLGWHPTEAGTYDEEVENYLLKALQHPKVIALGEIGLDYYWM
                     TADKEIQARVFKRQMELAKQLDLPFVVHTRDALEDTYQIIKEVGVGPRGGIMHSFSGT
                     LEEAQAFMDLGMHISFSGVVTFKKAVELQEAAQALPLEKILVETDAPYLAPVPKRGRE
                     NRTGYTRYVVDMLAQLRNQSVEEVARATYENARSLFRIDSEN"
     gene            complement(27632..27769)
                     /gene="rpmH"
                     /locus_tag="ACHU66_000511"
     CDS             complement(27632..27769)
                     /gene="rpmH"
                     /locus_tag="ACHU66_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR3388820.1"
                     /translation="MKRTFQPSKIRRARKHGFRSRMATKNGRRVLAARRRKGRKVLTV
                     A"
     gene            complement(27937..28932)
                     /gene="jag"
                     /locus_tag="ACHU66_000512"
     CDS             complement(27937..28932)
                     /gene="jag"
                     /locus_tag="ACHU66_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFR3388821.1"
                     /translation="MKFTGASVEEAIQNGLVELNIPRKKAHITVVAREKKGFLGFGKK
                     PAIVDIDVINETTVVKANQKAVRGVPSEINDLNEPVKSVSEATIDLGKVVAAVKEFEK
                     SGQTLDDDIKAQILKNEKEATTILEETGRIEILSEDLLTTTDATTSAKEDVNQEVVSE
                     NQADNFADLDIKIEPQYDIEQVVSEVTAYVQEILDDMDVEARIDTSHNRRTINIQVDT
                     NEPGRVIGYHGKVLKALQLLAQNFLYNRYERNFYITINVNDYVEHRAEVLQGYAQKLA
                     ERVLAEQEAYHTDPMSSSERKIIHRIISKMDGLTSYSEGSEPNRYVVVDIEGNHD"
     gene            complement(28952..29764)
                     /locus_tag="ACHU66_000513"
     CDS             complement(28952..29764)
                     /locus_tag="ACHU66_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFR3388822.1"
                     /translation="MKKKVKWAGLLVASLIVLTACGTGPVTSQSTDAWDRLIYWFASI
                     IQFLSINGQIGIGIILFTILIRTLLLPLFQIQMNSSRKMQELQPQLKKLQAEYPGTDM
                     ESRQQLYEATQALYKENGVSMRASMIPLFIQMPILLALFQALTRVEALKVGHFLWLNL
                     GEPDPFFILPIMAAVFTFLSSWLTNKSAIEKNMAMTIMTYVMPVMIFFFAVAAASGVA
                     LYWTVSNAYQVAQTLVFNNPFKIIAERQAKIDAEKERQAKIRRAQKKAQKKK"
     gene            complement(29748..30107)
                     /gene="rnpA"
                     /locus_tag="ACHU66_000514"
     CDS             complement(29748..30107)
                     /gene="rnpA"
                     /locus_tag="ACHU66_000514"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR3388823.1"
                     /translation="MKKSYCVKRERDFNDIFTKGNNVANRRFVVYRLPKEQKHFRVGL
                     SVSKKLGNAVMRNKIKRRIRHILIECQHQLVADDFVIIARKGVEELSYQEMKQNLIHV
                     LKLAKLYQEGVVIEKES"
     gene            complement(30178..31572)
                     /gene="argH"
                     /locus_tag="ACHU66_000515"
     CDS             complement(30178..31572)
                     /gene="argH"
                     /locus_tag="ACHU66_000515"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR3388824.1"
                     /translation="MEAKKLWGGRFEASLEEWVEEFGASIRFDQKLAKYDIQGSLAHV
                     KMLGQTGIVSQAEAQVIQAGLEELLEEYEAGKLVFDIRNEDIHMNIESLLTEKIGSVA
                     GKIHTARSRNDQVATDMHLYLKDTLFQVVDKLTNLRQILVDLAQEHVETIMPGYTHLQ
                     HAQPISFAQHLLAYYNMFSRDSERFAFNMQHTNVSPLGAAALAGTTFPIDRRMTSDLM
                     GFAKPYSNSLDAVSDRDFILEFLSNASILMMHMSRLCEEIINWCSHEYQFVTLSDTFS
                     TGSSIMPQKKNPDMAELIRGKTGRVYGNLVGLLTVMKSLPLTYNKDLQEDKEGMFDTA
                     ETVLISIDILAGMLQSMTVHKERMAQSTEKDFSNATELADYLASKGLPFRQAHEIVGK
                     LVLECSKAGHYLQDVSFETYQAISPLIQADIYDALSSKVAVSRRNSLGGTGFESIADQ
                     LKSAKEEIQNAKSL"
     gene            complement(31586..>32778)
                     /locus_tag="ACHU66_000516"
     CDS             complement(31586..>32778)
                     /locus_tag="ACHU66_000516"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR3388825.1"
                     /translation="TKEKLILAYSGGLDTSVAIAWLKKDYDVIAVCMDVGEGKDLDFI
                     HDKALSIGAIESHVLDVKEEFAQEYVLPALQAHAYYEQKYPLVSALSRPLISKKLVEM
                     AHKTGATTIAHGCTGKGNDQVRFEVAIAALDPSLKVVAPVREWKWAREEEIIFAKENG
                     VPVPADLDSPYSVDQNLWGRANECGVLENPWNEAPEDAFGITTSPENAPDTPEYVDIE
                     FKAGIPVAVNGEKLSLANLIQRLNVIAGKHGVGRIDHVENRLVGIKSREIYECPGAIT
                     LLTAHKEIEDLTLVREVSHFKPIVSNELSNLIYNGLWFNPATDALKAYLAQTQAVVNG
                     TAKVKLYKGSAKVVARKSPNSLYDEDLATYTSADTFDQDAAVGFIKLWGLPTKVNAEI
                     HKKS"
BASE COUNT         9312 a         7788 c         6511 g         9167 t
ORIGIN      
        1 tcattgccgt acacaaaatc acgtatagca aaaccaacat tgataccaat tgaaatatat
       61 atttttttgt tctcagcttt atgatgaatt tttattttat attgattatt tgtcatttag
      121 acccctcgct cacagttttc atcaatttat aatttttgtt agctataaaa taggctaatg
      181 actcttgtaa tgacggctct gagtacttaa cattatttaa gcttagaatt ttttgatcat
      241 tcaatcgtgt ttccaccata gcttctttca ttaacttaac atgcttagaa acaacatcaa
      301 tcttagaaga taggccaggc gtagtaacaa ttaaactgta tactcctact ttaggaccat
      361 tagtcaatat atagagcatc ttatctaaca actgactatc ctcttgtact aaaacatcta
      421 attcattcca aatgacaaca actatttcat catattcatc aaattcgata ttttcaaaaa
      481 cagaatcata tatttcatca atcattaata aacgactcga aggtgtaaac gaaacttccg
      541 tcccattatc agtaaagaac tcttcgccac taaccatttt tacagaataa tctgtttgtc
      601 ccagagtatt taaaaaacct cgcaaaaagt tatttctatg cctctcttgt tcataaatat
      661 atacaaagtt cccaacggtc aaatcccaac tcacagcctc caccgtttcc agatccagac
      721 caagcggaat ctgaccagag gcgatggctt tctggacgct ggcacgactg aggaagtcct
      781 cgcgagtcag ctcttctgga accattggga tagcttctgg aacctgaccc gtccaagcag
      841 ccttctgacg actcacttcc tcacgcatct gatccaccag ctctatatcg tgttccacac
      901 gaaggggaag tgccaactgg aggctataaa cctcctcacc tttatagagg gcccgtccct
      961 taatatcctc tatatccgca agaccagtag ccccgaccac actacggact tctgtcttat
     1021 catgttgctt caaggtcaac tggtgcttga agttagagta gaaggtcgca cgaaggttag
     1081 cctgacgact agccgtcacc acaaggtgaa gaccaatggc ctgaccctca cgagaaatac
     1141 gggtcaagag tttgagtaac tctgcctcaa aactctcatc cttcatagac tcgaagctat
     1201 ccaaggcgat gacaatacgt ggctccacct gtcctgtcaa ctgacggtac atggacaagg
     1261 tacccacacg atgttccgta agcagcttct tgcgacggtt gatttccttc tccaaaagac
     1321 ggataaactt accgatcttc tctccctgat ccaagaggaa gacatccgcc acatgtggta
     1381 aatcccccat tggaaccaga ccactcgtcc caaagtccat cagataaata tggagttcag
     1441 atgggctata gtgacgagct aaatccaaga ccgtagtctg taagaaggta gtcttaccgg
     1501 tcgctggagc cccatacata agcaaatgac catcctgaga taggttgata gacagcggtt
     1561 cttgggcctg ctggctcggt ttatccaaga gacctagaat aacctctaac tcctcattga
     1621 ctccagacca agttaactca gcctcatctc gtaaatcttc cacaaacaag cgcgaaggca
     1681 agggaggcaa ccatggttgc ggcaaggcct ggatggacag gcgctctgtc aaggctttca
     1741 gcttacttac gatggcttga agctcactcg gaacctcctt gatggactcc gcctgatcca
     1801 gacctgacag gtcttcgttg agaatctcat attgaccaat gtcattgata gcatagatgg
     1861 tatggtcctc aatcccctgc tcatccttat ctggctggta atcagcccca gaccaggcag
     1921 actggaagag ttcatagact tcattgtttc cgacctggag gtagccacga cccacctggg
     1981 taatctcagc cgcatctggc gtcttaagca tctccatgga gtcgctccga tccgccacct
     2041 tgagcgccag cttgaagcgg gagttggacc agatctggtc atccaccaca ccagatggct
     2101 tctgagtagc taagatcagg tgaatgccca aactccgacc gatacgagcg gttgagacca
     2161 gctccttcat gaagtcaggt tgattggtct tgagctccgc aaactcatcg gagatcaaga
     2221 agagatgggg catgggctca gacacctcac ccagcttgta cttcttctgg tattgattga
     2281 tatgattgac atcgtgggtc gcaaagagac gttgacgacg cttcagctcc gcattgatgg
     2341 acactagggc ccgcatggac tgggcgccat ccaagttggt gatggtccca agtaagtgag
     2401 gcaggtcctt gaagaggtta gccatgcccc cacccttata gtcgatgagg agaaaggcaa
     2461 catcatgcgg atggaaatta accgcaaggc ttaagatata agactggatg acctctgact
     2521 taccagaacc cgtagtacct gccaccaaac cgtgcggacc gtgagccttt tcatgtaagt
     2581 tcagatagac gatatcctcc ttgccacgaa gacccagagg caccgccaaa ctcttataag
     2641 gcgcatgctt ttcccaacga ctgacaacac ccaagtcctc aaaggtctca gccccgtaca
     2701 tctccataaa ggtcaccgtt tctgggatag aagacttaag gttctgtagg tgattaagcg
     2761 gagctaagcg gcgcaccaag gtctccttgt cgtagccaac tgggaagtgg tccaaggcaa
     2821 agtcaatctc acgcaactga ccttcctcca tgaccagctg accagtgttt cgatccttaa
     2881 tgttaatgat ggtcttgata ttctcagaca gagaagacag cacatcctgc acaaagacca
     2941 ggctacagcc cagatccgtc ggatcctctg tgaagaactc catgatgaca tggtccaaaa
     3001 tcaacttctc atccgtcacc aaaaccacat agtgagggct gaagagggtc gactcccgat
     3061 tggacttgtc ttccttttga gccctgcgca gtttcaagat ctgattgagc gagttgagca
     3121 cctgatcatg ggtcctctgg ttatagacaa agccacggac attcatgtcc tgcaaggtcg
     3181 catgaggcaa gaaccgcatc caatcccact gctccttctc ctcctctggc atgatggtaa
     3241 taaactgcac atcatgatag gaatggaaaa gtgctatttg gttgaccatg agttggagct
     3301 gctcaatcac caaagcccgc ggaccaatat agccgacagg accatgggac agattggcca
     3361 caatgggcat atctgaaatg gtcttgtttt gttcatagag ttgaaaaccc tccaactcca
     3421 agggatcccg cttgcccgac cgttccgtct gagcatagga caggtcataa gacaccggaa
     3481 cctcacccag acccaagcga taatacaaga aatcaaaatg aagaggcgtc ttctcataga
     3541 ttcgatggtg gtagctatct gccagatcca ccaaggtctc aatagcagga taatggtaaa
     3601 gctgaccatc tttctgcgac ttggtcagac cagccaactc cttggcctta tcagccagat
     3661 agtcatgata gagcccctct cggtctctca gctctttctg gtaatccttt ctccccttta
     3721 tatagccagc aatagacatc cccaacgtca caatcgacat ggcgatagtc gcaagcacgt
     3781 agagaccacg aggttggaaa atcatgataa ccaatgtgat ccccaccatc ataagaggag
     3841 gcaagattag tttcaacaaa ctgtcggacg gttttgaagg tgcattcgaa ggtgcgttga
     3901 tagccaccct gtcttctgaa cttcgataga taatccgtgg agagcgatgg tagtctggat
     3961 aatcctcata aaaagcatag cgcgatgcag acttacgagc caactcatca ctagcctcta
     4021 caggacccgc aaccaggacc tcatctgcat aaaatttgaa aaaagtatcc tcaaaagcaa
     4081 gctcatctcc aagagccagc tccatttccc ctttctccat cagaaggtta ttgatataga
     4141 tggagcccgc caaacgataa agcatccatg actggtcact gcgtttgagc aagacatgac
     4201 aacttgaccc tgcaaggaca aaactatttc ccacatgctg gccgatcgtt aattcctgct
     4261 tatcaagagg gtctaggatc acctgctccg acggaagaag atagaccacg ccccccgcca
     4321 aagacatacc agatacaagg tttccttcca ctccatcata gtcatagcaa acttgtactc
     4381 catctaacca aatcgtcaaa ggagtatcca gtgaggagac aaaaacctgt gccttttctg
     4441 tgccttgaaa aagaatcttt ttcccagcag acacatccgc cgcatagcga aaaccatctt
     4501 tataaaaaac tactgtctct gccatagctt actccccgct cgagctcgat ggactcgtcg
     4561 atgatgactc tgtagaagag gttgaactag atgtcgaact tgtacctgtt ttcacctgtg
     4621 acccatcttc attcaaaatc gtagttcggg tatcccaata cttttgatac tccgctttca
     4681 actcatccaa ggttgtttcc cgctcttctc ccgacaaagt cgtattttca cgaacctgtt
     4741 ccatttgctg gtaaagagca tacaaaatta aatccgaatc atccaaacgc ttggccaaat
     4801 ccaaagcttc ttggctatta ccacgaccaa tttcaatcca gtagtccaag tattgctcat
     4861 ccgatttgag cgttacatta tttaagataa cagaacgctg ttcattactc aaatcaaacc
     4921 cttggatgta ggcataggca agagtatatt tttgagtgta gggaagacta gaaacctcta
     4981 cccgctccaa ctcactaatc actccaggat agtccacctt gatgaaggcc gtatccgcat
     5041 ccagcatttt ttccttgaaa ggattgtgca tgaaaactag ataaatcagt ggcaagacca
     5101 ataatcctgc cagggcagaa agccagatgg tcgcatagcg atagagacgg tgcttagagc
     5161 gagaaaccct tgtcaaagtc gccttctcct cctccaactt atcctgaaaa tagccctgca
     5221 aaagtccttc caaactatcc atatcctctg ccttgcgaat ggtatctaag aactctggta
     5281 caggaaccac atccagcgca ccattgtaaa gatccataaa atcatgctct gtaaataagg
     5341 caaggatata agattttaat tgcttcaaca aatccgtagc atccaaggtc tgaggcacca
     5401 tcttctccgg gatagcccga taggctattt tggcccgacc atccttagta ataaagacag
     5461 atgacggatg cacgaaaaag gtgattggca atttcaaagc ctgcttcaat tctaaaacat
     5521 tgagagaaag tcgtagtttt tcagaaacct ctttctcctt catatcctca aaggtttgcc
     5581 caaatgcatc cagatgatag gtgaaatgaa ctgtatcttc atccaccgtc atttcctgtt
     5641 gtaaaaattg agggtggtgc agatccaaca agagcaggtc agatagctct tgcgttgcca
     5701 catcagaccg ttttacatca aggtgccact ctttatcacc ctttaaaaag acaaattcct
     5761 gtccaccaaa gacaaatgtt tcttctttca ccctaaacct ccaaaacctc tacaatatct
     5821 cccgttgtca caggataatc ggctaatact ttcccttcgt ccaaaatgaa ccccttatta
     5881 acaacatgga gttggtattt ttttctagca ttctcttttt cgaagatgct atccaactcc
     5941 ttgattaatc tcctaacctc aatgtgagtc ggaatccgta agtccctgat tcgtcctcca
     6001 tcatgtaagg taacattgat atgcgtctcc atctcatctc ctccaataga ccactagaag
     6061 attattcctg gcctacttca tccttaccca tcaaataagt tgctaaactt gcaagcaata
     6121 gaatgccaac tacaatagaa gcaaaaaaca ggccagagag aagccctgct ggagccccct
     6181 ctgtcccact atctaccttg gttgtcatca ccttatgggt ccgagtatca aacaaggtcc
     6241 ccacttgcaa aatatccgtg cttggtttct caccagtttg gaataggcta tttagctgat
     6301 gctctaataa tacttgatca gcttcttttt tggcagtcaa ttctgcttga tctggcgcta
     6361 aaaacaaaga ttggaccagt aaatcctgac ttcctatttt cttttctttt tctacttcaa
     6421 tgcgtgaact atcaatttga agactattgt ctccaagttc ggacgcttct atcccaaccg
     6481 gaagaaagag aaccagacaa ctaagaacaa gtgctatctt tttcatttaa tcctcttttt
     6541 tatttaggaa tttttccaca tcaacaaaag cagtcaaagc cgcaaaacca agccccaata
     6601 cgagcaccgt agcaatcact gtcagactgg tagcttcgcc atcaaagtaa ccagataaac
     6661 tattttctaa aacagaaaga gggttgatgg ctttcagtgt tgcaggcagg cctgtcaaac
     6721 tagcagttgt accaatggca ttagataggt aaataaagct gataaccatg aagaagctaa
     6781 tccccatgcc aagtccttta gcatttttta tcaatagata ctgaccttgt gctagtaata
     6841 ggctaaacaa ggtaatcatc agcacccaag tcgggatcaa ctcgctatca atctgcaact
     6901 gttgacttga tacaaccccg atcaatccac cgaccagaag tgatagaacg ctgagcacag
     6961 tcaccgttac taaatctgta tcctctagac ggctgacttt aaacttatct ttcacagcct
     7021 taatcacacg atgagtacca aaaacataag ctgcaaacag ggccaccatc tccaagagta
     7081 aaatccaggt gaaaggtctg taggtgttga ctgttgcttt tacagatgaa gacgattctg
     7141 tcaatggtgc ggacaagaaa tccaaaatca cttcatttgg aacaccattg tcataggtat
     7201 tgcttaagac agaagtaaag gtttttacaa agtcgccatt ttcagccaat tctgattcaa
     7261 acctctccat cagggcagtt gcttcagaag atagattctc tccgctttct tgagcagcct
     7321 gtacatcatt agtaaatgta gtgaagacgg tattgacatt agtcgcctcc tcaacgttct
     7381 gttctgaaag ggattttacc tcacctgtaa tagaaaggga agcatccaat tcagtagcga
     7441 gcgctgcaag ttctgtatca tgcgttcctt gaagagcagt ttgttgctct tgaccatctg
     7501 caatagactg agctcgtgcc ttgacatcct ccaaaaatgc cagctgattc gctatctgtt
     7561 gagccaaatc actatttgtc gtgctgatat attggagttg atcttttaat tcttgtttgc
     7621 gtccttctaa ctcagccaat ctttccttca aagcttgatc ttgtgcacgc gcttcagaat
     7681 aggattgtaa attagctgta atggagctcg taactaaaga ggtcaacagg gatttcgcgt
     7741 cttggttgaa gaaactatcc gtcaaactgc tacgttcacc agttacagta ttaacaggat
     7801 aatattgatc taataaacga ttagcttgat tatacttgga aatgatttca ctaacttttg
     7861 cagcataagc aacctctgct ttacgataag cttcctgttc ttttaagaag ttttcccaat
     7921 caaatcctgc tgttgtgact tccagattat taacacttac cttgacactc ccctgcgcca
     7981 tctcagttgt cgtgacaggt gtaccagtgg acggtgccgt agtcgatgca gtattctcct
     8041 ccgatgagct cgaacccgtc gttgacgaag ttgaagttga atccgttact accgttgttg
     8101 aaaccgtggt cgtctgactc ttttcatatt ggagagtgac ggttactgta ttatcctttt
     8161 gcaaagcaac aacttcacta ggattggtat agctgttgcc attcaattcc caagatacca
     8221 aatcaaaccc gctaggaatt tctaaggaaa gagtggcacg agttgttgga gtaggaattt
     8281 taaaatcagc agcagcttga agatgcggtt ctaactcttt agcaagagcc tgtaattctt
     8341 tcaaatcatt agaagctaca aagtgttcac caaaatgcgc ttgcgcaaat gctgcgtcaa
     8401 atcgaatctg actcgctgcc tttggatcca atttgtttaa atcagtagtc agatctgctg
     8461 gagtcaagct tggtaaacct gccaattgat tcaaaatcaa ctggtctaag ctcgtttgat
     8521 aggatgctag agaaatcggc aactctgtac tcttacccaa aaccgctcca atgtggattg
     8581 tttccagact atcgccatag taagtggtta aatgtgtttc agcaaagtta gaaatcgtag
     8641 cctcatgctc tgctaatttc gtactctcag cttcaattgc tacctgtaaa tctgcaatag
     8701 cttgatccaa cttttcaacc aaaccaatat agccactggc ttcacccgtc tcgtctatcg
     8761 ctgccgcccc caactgcccc gagagcacct cttgggtcgt tttaatcgtt gcaaacaact
     8821 gatccgtttc agcactgagt acagagctat tcattgctaa aagcgaagct gaaaattctt
     8881 cctgtgatag ttttccctga tttcgttgtt caatcaaggt agccaaatta tcttttagtc
     8941 ccgtctgagc agtatttagg ctatcatatg ccgtcacgtt gctagaaagc gcttctttca
     9001 aaagattatt agctgccaat gttgaatcag ccgtcgaagt caaattcgga aaaagtgtct
     9061 taaaatcaac agctgtttga tagagattgt tcctaaaaac agcaatattg gtcacctttg
     9121 tatctgacaa ggtctgaaca tttcgttgtg cctgataaag attcccgaga atgctggcca
     9181 tatacatatc aatcagctga ctctgtaggt ctgcaataat tccttttgcc accttaacag
     9241 cttcattctc tacctgcaaa ttaccatttg cattaatctt atacgtgatt gtcgctttat
     9301 ccacattggt attgttgata tccaagattt ttgttgaaaa atcgcttggg atggtcacca
     9361 acaattggta atgaccttct tttaggccct tgtcagccgt accacgactg acagttgacc
     9421 agttatgggc agtatttttt tctatatttt ttgcataact agcgccaaga ttatattccg
     9481 taccatttag ggttacagta ttatcttcat tgaccaaggc aacatccagc ttaatatttt
     9541 gttgccctgc ttcttgctct ttcttggaaa tactcgtgtt gttctgaaca gcaatattta
     9601 atccaatgat agatagaagc aaaagtaaga ccagcaaagc atttctccca tacttaaaaa
     9661 tgcgctgttt atccattttt catatctcct tcatttcgat aaaaaatagg aaggctggcg
     9721 ccttcctagc atttgaactt cgtcaaccct tagccattga tttgcgctgc aatgtctgca
     9781 tctgtttgct cgataatgtc agcaacttta ttcaattggg cattgatatc tcgaagcaac
     9841 tcagaaaatt caacaatttt tggcttaagc gcttcaaatt gagcatcaaa actgtcaaaa
     9901 gctgtacctt cccagttttc acgaataaca gattgctcat ttgacaaaga tgataaaata
     9961 gcatcaatgt tatctgaacc ttctgtatag cgaatcgcag aagtacggag ctcatctggg
    10021 gttaatttaa tacgtgacat atcattctcc ttttgtctta taattactaa tccattatat
    10081 aataaaaaag ccaaaaatac aaccatatat gcaactttat ttatagaaaa aataattatt
    10141 tagccaggtc aaaacataat tgtaaaaatt ttatactatt ttgactgcag agcaaaaaaa
    10201 ttgaaggaag atgtccgaaa caaaccttcc tccaatctga gctgaaaatt cagctttttc
    10261 tttatgcacg aataatctaa atagggacca ccttacatct ctttaagtgc aggttggtaa
    10321 atgttattca aaaatatttc ccaactctac atcgactcgg ttttccttga cattgatgtc
    10381 ggtcacagcg agcaaagact tgtagttagc tacgctacgg tccccttttt ggttttctgc
    10441 aaagacagcc acgcctacca aggttgagtc aaactcagac aagaggctaa tcataccatt
    10501 gatggtgcca ccatttttca agaagtcatc gacgattaag acacggcttc ctgccttcaa
    10561 actacgctta gacaggaaca ttttttcgat acggtcgcta gatcctgaca catagttgac
    10621 agaaacggtt gaaccttctg tgattttcaa atcacgacgc acaatcacaa acggtacatt
    10681 aaggacattg gcaacagcat tggccaaagg gaccccctta gtcgcaactg tcatgaccgc
    10741 atcaatcttt tcatctttga aagcatctgc aatgatacga ccgacatttt tcaaaatatg
    10801 tggtgtagac agcaagtcag atgaatagat ataaccgcct ggcaagatac gattactttc
    10861 ggccatctga tcacgaaggg cctgtgcaat ttcgacagac tctgccttag agatcgatgg
    10921 agtaaaaaca actcctccac tggcaccagt aatggtttca atatgcccga tagagctttc
    10981 ctcaaaagcc tttttaatga tggcaatatc ctctgagata gatgacttgg cagattcata
    11041 cttttccgca aaggtattca aactagttaa ttcataaggg tggttgatga ggtaattgga
    11101 aatgacaacc atacgctcac ttcttcttaa tttcataaaa tcatcctctt tcactttttt
    11161 ctattatatc acaacaaact acaaaaagcg aacttttttg attgaaaagt ccgtcttttt
    11221 cgtattttgt ttatcgctta catataccac tttggaagat gatgattaaa atattcatac
    11281 cattcagaaa tcttctgctc ttgcaacttc atcaatggaa tttgttcgga atctacttgt
    11341 tctgctcaag aaagagcgcc tagactatta ctgttacata aagagccatg agtctccaaa
    11401 attcctctgg aatagcgccg tcaaaatagg agtctacctg accacgcgca aaggctggag
    11461 acaaatctgc cgtgaaaatc aaacggttga attcttccca ggggtctcct atgtcgtagc
    11521 gatcaaagtc taatattttc agtttcccat ccgcacccag taggaagtta cctgtgtgaa
    11581 aatctccatg atggtaaggt ctcccttcaa gcaagtgacg attggcctga acaaagtcta
    11641 tcatggcttg accatttgga taggaatgac tagctgcttg gtagacagct aatttattgt
    11701 caatcttagc ctgataaaaa ctgttccagt cgcgttgact ttggtcaatc ggtagggcat
    11761 gcaaggttcg caaaaactgc cctgactgac agcccaaatc atataagaca gaatcagaca
    11821 agctagtagc cacttcattc atgtcctgac cttccaccca ttcatagagt gtataaactg
    11881 actcctcacc cacccaaaga gcgattggct ctgccacagg caaacctaga ccaaacagat
    11941 ttttaactaa ctgaaactct aatcgcttag cctcataggc tggtcgctct gctattctca
    12001 aaagaccaag gcgaccatct tctagccgga ccttgtactt tcggtccgtg gaccagcctt
    12061 tggtgagggg ctgtcgagac actatcctaa ctgccattag tcgatgttgg gcttatagaa
    12121 agcgcggtta tccatagcaa agatgcgatt ggtcatctcg cctgggccga ccttgtccaa
    12181 ggccgatagc atcatcatca tttccgcatc aatgttgtca atcatgtgga ggatttctgc
    12241 ctccataatc tgcggacgga ctgggcttcc atactccaag aggccatggt gactgaggat
    12301 aacgtggcgc agaacagtca cttcctccag actatcatcg atgcccaatt ccatcaagac
    12361 cttggtaatc tcttcatcaa tcaaggaaat gtgaccaatc agattgccac ggacagtgta
    12421 gccagtattg tctggaccag tcagttcgat caccttggct aggtcatgaa gcatgattcc
    12481 tgcaaagagc aggctcttgt tgagctggag gtagatgtca ccaattttat cagccaagcg
    12541 taccatggtc gctgtatgga aggacaagcc tgagtagaag gcatggtgat tggtcttagc
    12601 tgctggatag gaataaaatt ccttgtcgta cttgctgtag agagcacgga caatccgctg
    12661 ccatgtggca ttttcgatgc ggaaaatcat ctggctcaga tagtccttgg tgtccttgac
    12721 atccacaggt ggtttttcct tgaaatcagc agggtcgttg ggctcgcccg cctttggcaa
    12781 acggagaaca agctggttga cctgaggggt gttgttgtaa acctcacggc gaccctgcat
    12841 gtgaacaaca gtgccagcag taaagtcctt gattttccct ggctgggcat cccagacctt
    12901 gccttcaatc tcgccagtat catcttggaa ggtcagagct aggtagtctt tgccagctcg
    12961 tgtttgacgg acttcagcag tcttaatcag atagaatcct tcgaagaatt catctttttt
    13021 catttggtta attttcatta tcttcctcat ctaatgggga caggccaagc agtcccttgg
    13081 tttggtcatc ttcgtacagg tcaatggtac gaagactacg ctcgatggcg ttggtacgag
    13141 tggttaggag tttgtcaata ttgctgctgg cttgattgag ctgtttctgg gccttaagca
    13201 agaggtcaga aaacttgcca aattccagct tgacattgcc caagaccttg gaaatatcat
    13261 cggctgaccg ttgaatgtta agggtcttaa agccgacgga gagggaattg agcagggcag
    13321 acagggtggt cggtccagct actacgatat tttcttgacg acgaagctca tcaaagaaaa
    13381 tcggattgcg gaccacctct gaataaagtc cctcggttgg caaaaacaag acgccaaagt
    13441 tggtggtttc aggcggattg aggtacttgc tctgaatgtc cttggcaaaa cgcttgatag
    13501 ccgccaagag atttttgcgg tggagatcaa tctggtcctt atccccactt tcgtaagcat
    13561 cttccaagcg ataataatcc gccaacggaa acttggagtc aatcggcaga tagatatagt
    13621 ctccctcggt ccgtcccggt agcttgaccg catactccac acgctcacta gagcctgaaa
    13681 ctgtggcaaa ctctcgctca tattggctag gtgtcaaaat atcctcgata atctgtccca
    13741 gctgtaattc acccatgatg ccacgggttt tggtgccaga cagcacctta ttaagactcc
    13801 ccacatcacg cgccaccgtc tgcatttcac ccagaccacg attgaccgat tccaactgct
    13861 tggacaccgt ttcaaaggag gcctgtaggc gggtttgcag ggttttctcc aacttttcct
    13921 cgactgtttg ccgcatctct ttcaagcgtt tttcatttga ctcctgaata gcctgcaagc
    13981 gacggtcggt ggcatctcgc gtttccaagt ggcttctatt cagctcttga cggatgtcgg
    14041 ttagctgttg gtaaagctcc gtgcggagtc tttccacctc ctgatgaatg gcctgttgct
    14101 gtttgagagt tgcattttct agttgatagc tgagctgatc agacaagttg tctgctgtat
    14161 cttcggcttg atcttgcaaa agcagggcca agctctgcca ttttccataa agaaaaacca
    14221 aggcaagcaa gaccagtatc agcaaaataa gtagtacaat atccatctaa ttcttatcct
    14281 tactgtagat caggaccacg tagccctgat ccagttgaat gtctatgtct ctatctataa
    14341 attcgttaga agcatagcat tttttgaaaa aataattgct ggcatccagc gggtacttgg
    14401 catgacgaat ggtcaggcga ctttggtccg acggcataaa ggaaatgtac ttcataccag
    14461 caatcggtga caaacggtgc tgacctgcag gtcgaaatcg gacgatgttc tgactatcca
    14521 ccaattcaat ctgctccatg tagggtgcca agtcgggttc tgcaaccaga aagagattgg
    14581 ccatcatatg atccatgcga ccacccaagg ctccgtatat gcgaacctgg gcctgcggat
    14641 aaaccttgaa gacttccttt aacgctaact ctaagtctgt atcatctttt tcagcaggag
    14701 cttgcaaaaa acgctctgct tgatccttta tccgccccaa ctcctccgag gtcaccgagt
    14761 caaaatcacc gatagcccag tcaagaggca ggaaatggtc cagaagacga agagagcctg
    14821 catctacccc cacatagagg tcggcaggct caggaaagca gtcaaaggaa ccgcctgcaa
    14881 tgacagcaat cttaatcatg gagggcagct ctcaatttgg caacgttggc atccaagtct
    14941 cctttgaaga ggtaggatcc agccacaaag acattggcac ccgcattttt ggctgaatga
    15001 atggtcttat cgtcaatccc gccgtccact tcgatgtcaa agttcaagcc tttagcttca
    15061 cgcaatttaa ccaaatctgc aatcttctca gccacttctg gaatgtaggc ttgtccacca
    15121 aaacctgggt tgaccgtcat gagcagaacc tgatccacca agttgagcac aggctcaatg
    15181 gtcacaagag gagtgcctgg attgatcacc acacccacct tcataccagc agcacgaatt
    15241 ttttggaggg tcccatgcaa gtgaaccgtt gcttctgcgt gaatagtcaa gatatccgca
    15301 cccgcacgtg caaaggtctc gatatgattt tcaggattgg acaccatgag atggcaatca
    15361 aaaaccagct tgctatgagg acgcatagct gctaccacat ccgccccaaa gctaatattt
    15421 ggcacaaagt gaccgtccat gacatcaatg tgcacatatt ccacacctgt cttttctaga
    15481 cgcttgagct ctttttcaaa atttgcataa tctgccgcca aaatagacgg tgcaatctta
    15541 taatgtgaca tacagaacca actttctatt tgaatttctt acttaccttg gtataggttt
    15601 cacgctgatt ttggatttcg ctgaggaatt ggagataatt gtcaaagcga ctttgggaaa
    15661 tggcagagct ggcaacagct tccttgaccg cacaatccgg ctcatgggta tgggtacagg
    15721 tcctaaattt gcagtcctga ctgctttccg caatctctgg gaaacaatct gtcagagcct
    15781 ccgcctcctt gacttcatag tctagagatg aaaaacctgg cgtatccgca attttcccac
    15841 cgaagacatt gtagaaactg accgcacggg tggtatggcg accacgcccc agactatctg
    15901 aaatagcacc tgtttccaag gctaattctg gcgcaatgcg attgagaagt gtcgatttgc
    15961 caacccctgt ctgccccata aagaccgtca ccttatcctg caataatggc gtcaattcct
    16021 ccaaactata gacaaaagga tagctaattt tctcatagat agccttgaag gcatccatct
    16081 ccgtccgatc ttctaccaaa tccatcttag agatatagat aatgggatcc atatccttct
    16141 gttcaaggag aactaggaaa cgatccagta gattggcatt aaaatcaggt tccttggccg
    16201 acatgataac caccgcctga tcgatattga cgataggagg tcgaaccaaa ctatttttcc
    16261 gctcatgaat ctttaatata tagccttctg agttttcctc cgcagaaaag tccacaaaat
    16321 cacccacgta gggcgtttgt cccttcttac gaaaatttcc tctggctctg gtttgataaa
    16381 tctgtccatc cgcctcgaca tagtaaaaac ctgccaaggc cttgataatt cttccttgca
    16441 atgaagactc ctttgtgtgt acttgctact attataccaa atttcctaca aaaaaaccgc
    16501 aagcggtttc gattttacat tttcctctag tcatttcaat gtaaaaatag tataataaaa
    16561 agcaagcgga ggtggtggaa cggcagacac gcatgcttca ggcgcatgtg cccatcgggt
    16621 gtgagggttc aaatcccttc ctccgcatta tttataaagg ataactccaa gtcaaagttg
    16681 atgacttggg ggcattttta tatcttctcg ctcatactca atgaaaatca aaaacagact
    16741 agctccaaag gttttggaaa cctttggagg ttggaaatag agcgaacgta gttcgtttct
    16801 acttacgcag atagaaccct gttactattt tgtttcaagg taacaggctg aaaggctcca
    16861 ctggagcctt tcactcatca aatcaagtca acaatgtctg attttgattt tcgaagaata
    16921 ttaattgctc tccccaatcc gtaccacccc atcataacgg ctaaggacct cctcggacat
    16981 gcggtgggaa atatgaatga gcgtcttatc agctagtgag gtaaggtaat tttcaatcac
    17041 tgcataggtt tcctgatcca aggcagacag actttcatcc agcaacaaga tatccttatc
    17101 ttgcagcaag gcacggatca agagcaggcg ttgcttttgt ccgccagaca gtccgctatc
    17161 atcactaatt tcctcgctca aagaagcata acttgggaaa cgactgtcaa ggttcatggt
    17221 cttaatgaga aacatcagcc tatcttctgg aatctcccgt cccatggtca agttgtatag
    17281 aaccgtatca tggaaaaggg atccctcttg tggcagatag gcaatcttgt cttgaatggc
    17341 ttggtaggat aagtctgaaa gagaggtttt atcaacgtaa atatcccctg cttgcagttg
    17401 gctattcttg gtcaaaataa gagccaagga agatttgcca gaaccacttt ccccaagaat
    17461 taaatacttc ttgccacgct caaacgctac ggtcaggtct tcaaagacct gtttttcacc
    17521 gacagcataa ctagccttga ccagttgaat actctggaaa gctgaagcta gttcctgtcc
    17581 cttcgtttct tgaccaaaag tagatagctc aaactcttgc tttaatttct taacggcatg
    17641 cctgtccaaa aaggctgccg caatgctatt aatgggatcc accaactgct ctgaaatggt
    17701 cagaatagct gtcaagccac cgatggtcaa ggcaccagaa aagacttgaa aaccaccgat
    17761 aaagataatc gaaagagtgg tcagataaaa gacaaaaccg actaagagcc gcatggttac
    17821 tgtcgttcga gtggcctgat tgaccttgtc attgactgct tgatagcgat cgttcagacc
    17881 atctaaaact tgctgtcttt ggtggctatt tttcacttgc aaataactca gaaagacatc
    17941 tgaaactctt gtattataga cctgctgact gtccgaaatc gcttcttgat tgagccttct
    18001 ccgcttttta aacaaatagg gaatcacaac cggcaagaat gagatcagaa tcaagaaaat
    18061 agcggtcaga ccatccaagc taagcaaggc caaactggca atgccaaagg tacaagcacc
    18121 ttgaaaaatc gtaaagaaag cgtcgtaata atcttctttc accgtctcca aatcattatt
    18181 gagctgggaa atataggccc ctgccccctg ctcaacaaac tgcctaaaag gtaaatcaaa
    18241 aattttccca ataaaagtcg cctgcaattg gaaacctact tgattgagat agctgacatt
    18301 ggcaatctga cgaatgtatt caaagaccag catgacaagg acagttagtc ccatcagcac
    18361 aaaataggac acaaaggcct gtctatctcc agctgcaata gcatcaataa tatagcgata
    18421 ttggtacaga aagccaacat tgagcagggc tactgcaaaa atacaagtta aggttagcca
    18481 aaaattcctc tttttcattt ctcctccact gtttgataga tttctaagca aagcaataaa
    18541 aaaatctgca ttaaacacct ttattatacc actccttccc atatttgtgt ccattttcac
    18601 ataaagaaaa agaagctagg cataaagtcc agcctcgctt ctcagagttc gtgtcaacat
    18661 ctcagcgcag tggttgattg gcagatttgg tcgtgttgca cactccaaat ctgacctaat
    18721 caactgtgcg ggggtgggaa gacgaactct ttattgacca gtcgagttct ttcccactcc
    18781 cctattttta ttctgtcgtt tcagtcgcta ctttgaaaaa atctttgtat ttcaaaaggt
    18841 agccttttcc tgtggatagg tcatattgga ccagtttgag ttcttctgca actttcgcat
    18901 ctcgctcagc ctgtcctact cggtccttat gaagtacttc tgtcaagagg gcatagtaag
    18961 gactgacctt gttattggtc gtctccagca tcagtgcatt catgtcgcta gaattgacca
    19021 aatcataatg gtagtcttct gtttcaaaat tactccacac gaaataatcc gtcttgtact
    19081 gcgcacttgg gtccgtcacg aaagctgact ctggatacaa acctggtagg tggtcaccat
    19141 aaaaaacaac ggtcactttc ttatccaagg tcttgagctg gtctagaaaa tccctggttg
    19201 cctgatccgt aaaggataga agacgaacat agctattgag gttggcattc tcctcagccg
    19261 tgaaaccctc acccgaagct gtaatacttg caggctcagc agagttccac tgaacatggt
    19321 tctgcatggt aatggccgag acaaatgtat ttgttcctga acgtacctgt tccaaaatca
    19381 aatcataggt ctttttatca ctgacaaaat tcccctgata atcttcagcc gtcactgcaa
    19441 gtggatactt ggcactattg agcgtataga actgctcaat ccccaactgc ttatagatag
    19501 aattacgatt gtaactggta tcgaagtagg ggtgaatagc gacacgattc tctgctggat
    19561 agaggtcact gatacttggc aacttcttca tatttggcgc cacatccaaa tagactgaag
    19621 aaatggatga gctgtaattg tagaagggca gaccactata gacctgaaac tcgatattag
    19681 ccgtaccacc accatagtga tcactcttca tcaagccact ggtagtctga ctcataatgt
    19741 actcaatatt tggaaggaca ttttgactaa gggttacacc gggaatccga cgcgggtcag
    19801 ccaaactctc actaagaatg tagataaccg tctggtcagt caattgacct gtccgttctg
    19861 cattgatttc ctcagccaga gcggtatact tcttcacaat ggccttcatg gtcttctcag
    19921 agtaattctc aggcatttcc atactggtct tgctgagttg ctgcaaccag aggaatgaaa
    19981 gagtgcgata acgagccaca aaggcgtagc ccttccagtc tacgttccgc cagttattga
    20041 cctgagaaag aataggaatc cagccagcaa tcttgtggtc tttttcattt cgaatagccc
    20101 agcccattcc caaaattagg gatacgatga ctgaaattcc tgccagtcgt ttccagatag
    20161 cagcgacaat ctgccctcgg aaatacttct tgtgaatatg tcggtagata aacacaaaga
    20221 ggactataaa gacgagcgat accaaaataa cacgtagact gataaagctg agaatcatcc
    20281 ccaagttccc gacccatttg aaatcattgg gggtcaatgg ctccgaacgg tagcggaatt
    20341 tgagaaaatt ggccgtaacc aaaatgacgt ttagactagc aatcacggca acagataaga
    20401 tttggcggtt gataactagg ctaatgatga cattgaggaa aaagaggcag gcaatctgga
    20461 atacaatagc ccctggaaag agatgacgtg tgaaataaga accagtcgct ccagccatag
    20521 aagcctgata accaatatgc gatagcgtag ccagtgccaa actgataaag agcatgctgt
    20581 ggacattggt tcgattggcc ttaaactcta caaatgcctt atgaagatag cgaagaaaaa
    20641 tgtaggtccc tataaaatga ataggtgcaa acagcggtgt ctggctgaaa aactctatgt
    20701 aagaaccttt ctcaaccaaa ctctcataat ggctatcttt ccagtaagat atggctagag
    20761 gactgagcga gagtaagctg gtcaagagca aatagccatc tggtaccaag tgattggctg
    20821 accactggct taatttttct cgaattcccg tcttcttccg ccataaaaat gccaacaata
    20881 caggaagagc caacatcaat agaagctcaa attgaaagag attattccgg aaaatattcc
    20941 agggttgata cttcttggca ttgagccgat agaccaggtt gacaagatat agaaaaccta
    21001 gaaaatagct gacatacttg gcaaaggttt tggtctttat aaatttctca tatttatcaa
    21061 gcaagaccaa tacataaaat gtaaaaataa gataggcaat caagggtagg ggaataaacc
    21121 agagtggaaa agatacatca ctcagtaaaa gggctcgccc catagccagc aagacggtca
    21181 ttaccatgac cagaataaat tgcttggttg ccttgagttt gatttgtttc atagcatttc
    21241 tctcttagga aatatttgat tttcggtaga taaaggtata ataaatcgac caaagtatcc
    21301 agccagtcaa actgataaag ataactggca ataaccacca ttgttgctta taggagagga
    21361 ttagggtgtt cttccctttt tggctggtaa ccgttggcac accaatagtg gaaagataat
    21421 aatccatctt ccctaacctc tttccattca atgtcaattc actatctcgg taaataacaa
    21481 tcggtatttg gatttcctct gattgatttg ctgaccaact tatttttaag ctatccccaa
    21541 aaacttgctt attaaattgc tggttaggta aaataatctg gtcacggtaa cgatcataac
    21601 gagatttagc ctcatttacc gcacgaacat tctcttcttt ttcagataac accggcacat
    21661 agtcgggagt tgatttctga gccagttgaa caaactcacc taaatttttt gaatgcaagg
    21721 atgcacgtaa ttcttccgtt gttccgtata gagtagtatg aagcatatct tgtaagaatt
    21781 ctgctttgtt gtactggtca ttgactgctt gattatattg atcccaagta aaaccaacac
    21841 caattatggt agataagatt gtcaacccca aaaacaactt tttacgtact tcgtaatctg
    21901 ttaattgaag accaacaatc gtcagaagta aagcattagc atacaaaaag aatctaaagg
    21961 gaaattggat taaatccact aatccaatac ctttaccagc aaatagttgc cacggaaaaa
    22021 gatttgttga aaaaataaaa aataccagaa aagttactgt cagccccctc aatatcaccg
    22081 attgcttctt ccattttttc agcataaaat aaagaaaggt tgcaaaaatg attggtagag
    22141 agtatggaaa gtataagaaa taagccttag accaggtaac tgttgactga ataaactttt
    22201 tgtttataaa tggtgctgct aattcatttg ttgcatttac atataataag ggtaaccaga
    22261 tatttacagt taaaatcaga aaaactccaa ccgaaaacag tacatctttc attacgttcc
    22321 atttgtcttg cgattttaac caaccaattg aaaaaagaat tgcataggct aagatgagaa
    22381 acagagccga cagcatgtgt atctgcaaca ttaaccctac agctcctgcc atcagaaaaa
    22441 taggaacttt accagttttt aggaatcgaa ctgctggaat caagcaaagc ggaaataagg
    22501 ccactcccca acttgaaaat ccttgagcta cccaccagta ttgaacagag taggtagtca
    22561 cgaaaaacat ggataaccca acagatagag aatagcgtac tttcacttct cgcattagaa
    22621 aataaagcga agtggcactc aaaactccta aaagaagatt agacagtagt tgatatcgat
    22681 accaagttcc ggccaacaac agcaaggctc cttgcaaata ggctagatat ggaccatata
    22741 gagcattgac aatccttcca gattgctgat agccatgcaa gctcattata taagaaaaat
    22801 ctcctgactt aatttgcata gctgtatcat aaaaacgatt ataatgaaaa acaatatcgt
    22861 ctcctgtcac cactgctttt gacaaaatct gtggcaatag aagggtgagc gaaaaaagaa
    22921 aaaatacagt tactgcaaca atatctggat gctttttcag taattttttt aacataagat
    22981 accttcataa aaaaagaggg aacccctctc ttgtttttgt aagaacctgt actcagaagt
    23041 gaagtgatta tcaataagag atagtttttc gctaatcaag gcagttttat tgagagaata
    23101 ctgactgtac tttgaaaaaa ctaacgatga ctagcttaaa aactatcctt aagtagaagt
    23161 gctgaactgt gaatacgggt tcttactatt ctgctcattt gcagacaata attccttaaa
    23221 atttatcaac tcccagtata acaagctttc cccccactgt caacggacaa gatagattga
    23281 aaatggtttt aggtaaaata aagattgatt gcagatttct ccgcaatcaa tacttgctta
    23341 taaaccaact tcctgaaggc tatcagacaa acgtgcaaag tcagccatgg aaagtgcttc
    23401 tccacggaca ctggcttcca gctctgccat gccaagtgcc cgcgtcagct tgtccttgac
    23461 ctcgtcggac ttgccaaaat ggttggtcag gttgttccac agggtcttac ggcggtgcac
    23521 aaagctggcc ttggacactc ggaagaagaa atcttcatct ttgacgctca ccagaggttg
    23581 ctcgcggcga accattttga gaatggcgga gtccacgttt ggagcgggca caaagacggt
    23641 gcgaggcacg atgaaagcca ccttggcagt catgtagtac tggaccgcaa tggacaggct
    23701 gccataggcc ttggtgtttg gctcagcaga aatgcggtcg gccacctccc gctgcatcat
    23761 gaccacaaac tcgctgaagg gaatcttgct ctcaatcagg tgcatgagga taggggtggt
    23821 gatgtagtag ggcaggttgg ccacgacctt gataggcaga tctggattct tgaagttctg
    23881 aatctgggtc tgcaggtcgg tcttgaggat atcttggttg accacagtca cattgtcaaa
    23941 gcgacccaag gtgtccgcca aaatcggaat caggcggtcg tcaatctcaa aggccatgac
    24001 ctcggcggca ttctccgcca aaaactcagt caaggctcca attccaggac cgatttcgat
    24061 gacattgaca ttctggtcaa tctctgccgt gttcacaatc ttctgtaaaa tattagtatc
    24121 ggtcaaaaag ttctgaccaa aggatttctt aaaggtaaac ccgtggcgtt ccaggacggc
    24181 acgagtaacg tttttgtctg cgattctcat gtgtgttctt tcttgttttt tatttcgcca
    24241 tttcactcaa tcgtgaaata acacttagtt ttctcatcgt cgctgtaatg tccagctgtt
    24301 tttctaaata attggtattt agttttttct ttcgagcatc tctcggaaga tacatgtata
    24361 gacattgact tcccagacgc aactcctcat cgccaaagtc ttggtgcaat aatttgttta
    24421 atttttcacc atttatctca tcattggtaa agaccaaatg aacacgagaa ggatcatagc
    24481 cctcaccaaa agaattttca gcaactgctt gtgttaactc catctgatgt ttgacaatga
    24541 tcgctaactc tgcacctatt ttttctttaa tggtttgatg gataagccga ctgagttctt
    24601 catctaatat atctgtttca caaacaacat ttccgctttg aatataggtt tggacagaag
    24661 ttaaaccagc ttccgtcaaa atttccgcca agtaagacat cttgggaatt ttattcttgc
    24721 caacaggggt aacacctcga aggaggacga cttttttcat ttgttaatcc ccctcaccta
    24781 taatgatcct ttagcgacag gatgcacaac cgatcagctt ccaccatata aatcaaccga
    24841 ttctccgcat caattctccg agaccaggca ccttgataat catacttcaa aggttctggt
    24901 ttggcaatgc cttcaaaagg atgtcgctgc atatccttga taatcttatt gattctcttc
    24961 agtgttttct tatcctgacc ctgccaataa agatagtctt cccaggcgtc gtctgtaaaa
    25021 tggattccca tgttagtccg cctctatcag gtttcgttct tgggtttgtc ccgttttgac
    25081 tttagccata ccacgtagga ctttttgaga cagataagtg ttccgagcaa cagcgagggt
    25141 ttcttgtaaa ctatcccact cactctttga gagtacaaca atatcttcct ctggattttt
    25201 attaaccacg accaaaggtt caaactcgtc attgaccttt ttcatatagt cctttaagtg
    25261 atttcgaaaa tgggaatata caatagcttc cataagcaat acctctcatc atttctaggc
    25321 ttactatagc acagaaaaaa agagttgtca aacttattgt acaactcttt agcgattctc
    25381 caccttccac tcgccgtctt caaaccagta aaaattctcc gtcagctgtg gctgttcaaa
    25441 gatttccagc atggtttcaa agccgtcagc cagttgaaca ttcttcaatt ctgcacggtc
    25501 actccagaag acctccccct catctgaaga ttgaatagaa cctgaaaaat gactagtttt
    25561 atagcaaaat accagataac gatagtggtc agtcggatcc gtccagtttt gaatgccaca
    25621 gagttcgaga ttggataccg tcaagcctgt ttcttctttg acctcgcgga cgacagagga
    25681 gacaatactc tccccgtgct caacatggcc cccagggaaa gtcacaccag gccacttttc
    25741 agacttccta tcctggacca acaccctccg accgtctgta atcatgcaca tattggttaa
    25801 gacaacttgt tctcgtctag acattcccta tctccccaaa tcatcaaaat aatagagatt
    25861 caaaaattct tcctcagtaa ctggacgttc aaaattgtaa aaccagcgat agacttccac
    25921 ggctggtcca tcaaactcta ccctgtcgtc catttcttgc acaccggcaa tatgaaacaa
    25981 acggctctgc ttccaaatac catccgcatt gacatagcca tcttcaacta aaccacgcaa
    26041 atagtctgct agtttgttct tatcaacctg ctcatcatcc caaacaagaa cattggccgg
    26101 cagcaagtag gtcacttctt cataagatga gtaattctca taagtaggct caaactcgtt
    26161 tttaaccaag cacttaacag cgtagaccct atccatgata gctctccata gcctcctcaa
    26221 cctccaccaa acttatccca aacaattcca atcgtttgag gagttgtttg ccgttgcagt
    26281 agccgatgcg gagcttctct cccagatact ccctccgctt gcggctttca ccccccatca
    26341 gcaatcctag gcgcattaga tctgacttgc tgatgtcaaa gttattttca tcatcatagt
    26401 agccacgcac gcctgcgagg gccttttcca agtcctcaaa gctggcgtgc tcaatgccca
    26461 aggaccgccc tgttgttttg gacttaggtg tagcctcgtc ccgctgtaag aaggcgtgtt
    26521 tgaccgtggg aatggcctgc ataatgattt ttcgaatccg ctcgccattg tagtcggggt
    26581 cagtaaagac aatgacgccc cgcaggtcat ggagtttctc gatgcgttcc aagtcatcgt
    26641 ctgatatggc agagcctctg gtttcgtagg tatccacctc gtaaaatcgt tttaggtttg
    26701 ctgtatcatc cttgccctcg acaacgagga cttcttgtat cttaattttc tgagtcaatt
    26761 ctgaacaagc tccttgcatt ctcgtaggtc gctctcgcca cctcttcgac cgactggttg
    26821 cgaagctggg ccagcatatc caccacatat ctggtgtagc cggtgcgatt ttcccgacca
    26881 cgcttgggaa ctggggccag gtagggcgca tccgtctcga ccaggatttt ttctaggggc
    26941 aatgcttgcg ccgcttcctg aagttcaacg gcttttttga aggtcaccac tcccgagaag
    27001 gagatgtgca tgcccaggtc catgaaagct tgagcctcct ccagagtgcc agagaaggaa
    27061 tgcatgatgc ctccacgcgg accgacgccg acttccttga tgatctggta agtatcttcc
    27121 aaagcatcgc gagtatggac cacaaagggc aaatccaact gcttggccag ctccatctgt
    27181 cgcttgaaaa cacgagcctg gatttcctta tccgccgtca tccagtagta atccagtcca
    27241 atctctccca gggcaatgac cttgggatgc tggagggctt tgagcagata gttttccact
    27301 tcctcgtcat aagtgccagc ctctgtcgga tgccagccca atgtcagata aatttcaggg
    27361 tgctggtcag caagctccat agccttgtca atggttgcac ggtcaaaacc gaccacattg
    27421 tggagggtga cccccatctc cttggcaaag tctaattctt cttggacccg accgtcaaac
    27481 tgctcgacgt tgaggtgggt atgggtatca aaaatttgta tcatcgttcc atcactttct
    27541 ttcctactat tgtaacatat ttcagaagat tcttatgaga agaaaaaaaa ccagcagact
    27601 cgagtttctg ctggcttctc ttttcatctt cttatgctac tgtaagaact ttacgtccct
    27661 tacggcgacg agcagctaac acgcgacggc cgtttttagt tgccatacgg ctacggaaac
    27721 cgtgtttacg cgcgcgacgg attttacttg gttgaaaagt acgtttcacg atgaatacct
    27781 cctcttagat tcttgtattc gtttagccgg ctagttttga tcagttacta aacataccaa
    27841 actattttat ataaattctg ttcgtttgtc aagctattct gccatacttt taggaaagaa
    27901 tcagaaaatc attgaaaaaa gaggactagc ctctttttaa tcatgatttc cttcaatatc
    27961 tacaacgaca tagcggtttg gttcgctacc ttctgaatag ctcgtcaaac catccatttt
    28021 agagataatg cggtggataa ttttacgttc gctacttgac atcggatctg tatggtaggc
    28081 ttcctgctct gccaaaacac gttctgccaa tttttgtgcg tagccttgaa gaacctctgc
    28141 gcggtgttct acataatcat tcacattgat tgtaatatag aaattacgct cataacggtt
    28201 gtataagaag ttctgagcca aaagttgcaa agcctttaag acttttccgt gataaccgat
    28261 aacacgacct ggttcattgg tatccacttg aatattgatt gtacggcgat tatgacttgt
    28321 atcaatacgc gcctcaacat ccatatcatc cagaatttct tgtacataag ctgtcacttc
    28381 tgaaacaact tgttcaatat catactgggg ctcaatctta atatctaaat cagcaaaatt
    28441 atctgcttga ttttctgaga cgacttcttg atttacgtct tccttagcag atgttgttgc
    28501 atctgtagta gttagtaaat cttctgaaag aatttcaatc cgacctgttt cttccaaaat
    28561 cgttgtcgct tctttttcat ttttcaagat ctgcgcttta atatcatcat caagcgtttg
    28621 accagacttt tcaaattctt ttaccgctgc aacaactttt cctaggtcaa tcgtagcctc
    28681 tgaaacgctt tttactggtt cattgaggtc attaatttca cttggaacgc cacgaactgc
    28741 tttctgatta gccttaacaa ccgttgtttc attgatgaca tcaatatcca caatcgctgg
    28801 ctttttaccg aaaccaagga aacctttttt ctcacgcgca accacagtaa tatgtgcttt
    28861 ttttcgagga atatttagct ctaccaagcc attctggatg gcctcttcaa cacttgctcc
    28921 tgtaaatttc atcttctctc tcctagtttt cctatttctt tttctgtgct tttttctgtg
    28981 cccgtcggat ttttgcctga cgttcttttt ccgcatcaat tttcgcttga cgctctgcta
    29041 ttattttaaa cggattattg aacaccaagg tttgagcaac ctgataggca tttgacactg
    29101 tccaatacaa agccacccca ctagcagctg ccacagcaaa gaaaaagatc atcacgggca
    29161 tgacataggt cataattgtc atagccatat tcttctcaat tgctgattta ttggtcaacc
    29221 atgagctgag gaatgtaaac actgcagcca taatcggcaa aataaagaac ggatccggct
    29281 ccccaagatt taaccaaagg aagtgaccta ctttcaaggc ttcaacacgt gtcagagctt
    29341 gaaagagggc caaaaggatt ggcatttgaa taaagagcgg aatcatggac gctctcatgc
    29401 tgacgccatt ctctttgtag agagcttgcg ttgcttcata aagctgctgg cgactttcca
    29461 tatccgttcc tggatactcc gcttgcaact ttttcagctg aggttgaagc tcctgcatct
    29521 tccgtgagga attcatctgt atttggaaca aaggcaacag aagggttcga attaaaatgg
    29581 taaaaagtat aataccgatt ccaatttgac cgttgataga taggaactga ataatgctgg
    29641 caaaccagta aattaatcta tcccacgcat ctgtagattg actggtcacc ggccctgttc
    29701 cgcaggctgt taaaacaatt agagatgcta ccagtagacc tgcccattta actttctttt
    29761 tcaatcacaa caccttcctg gtatagttta gctagtttca aaacatggat gaggttttgc
    29821 ttcatttcct gataggacaa ttcctccaca ccttttcgag caatgataac aaagtcatct
    29881 gctaccagtt gatgttggca ttcaattaag atgtgacgaa tcctgcgctt aatcttattt
    29941 cgcatgacag cgtttcccaa ttttttgctg acagataaac ctactcggaa atgcttttgc
    30001 tctttaggta atcggtagac aacaaacctc cgattggcga cattgtttcc ttttgtaaaa
    30061 atgtcattaa aatctcgttc acgcttgaca caatagcttt ttttcaaact gccactccat
    30121 ctttcaaaat tacttctatt ataccacaaa attcaaaaaa gccaataaac ataccgttta
    30181 taggcttttt gcgttctgaa tttcctcttt ggcacttttc aactggtctg caatcgattc
    30241 aaaacctgtc ccgccaaggg aatttctacg actaacagca actttggagg acaaggcatc
    30301 ataaatatct gcttgaataa gaggcgaaat agcctgatag gtctcaaatg atacatcctg
    30361 gagataatgt cctgccttgc tacactccaa gaccagtttt ccaacaatct catgcgcttg
    30421 gcggaagggc agacctttac tagccaaata atcagccaac tctgtagcat ttgaaaagtc
    30481 cttctctgtc gattgagcca tcctttcctt atggaccgtc atagattgca acatccctgc
    30541 taaaatatca attgaaatca aaactgtttc tgccgtgtca aacatgcctt ccttgtcttc
    30601 ctgcaagtcc ttattatagg ttaaaggaag ggatttcatg actgtcagta ggccaactaa
    30661 gttcccataa actctacctg ttttcccccg aatcagctca gccatatctg gatttttctt
    30721 ctgaggcatg atggaagaac ctgttgaaaa ggtatcggat agggtgacaa attggtattc
    30781 atgtgaacac cagttaatga tttcttcaca caaacgcgac atgtgcatca tgaggatact
    30841 ggcatttgac agaaattcca agataaaatc acggtcagac acagcatcaa gagaattact
    30901 atagggcttg gcaaatccca tcaaatcaga cgtcatccga cggtcaatcg gaaaggtcgt
    30961 ccctgccaag gccgccgcac caagtgggga tacatttgta tgctgcatgt tgaatgcaaa
    31021 gcgctcactg tctcggctga acatgttgta ataggctaac aagtgctgag caaaactaat
    31081 gggctgggca tgttgcaagt gggtatagcc gggcatgatt gtttccacat gctcctgtgc
    31141 aaggtctacc aaaatctgac gtaggttggt caatttgtct acaacttgaa agagggtatc
    31201 cttcagatag aggtgcatat ccgtagccac ttggtcgtta cgagaacgag ctgtatggat
    31261 tttgcctgca actgagccta ttttctccgt caatagactt tcaatattca tgtgaatgtc
    31321 ttcgtttcga atatcaaata ccagcttgcc agcctcatac tcctctagca attcttccag
    31381 accagcttga atgacctgag cctcagcttg agagacaatc ccagtttgcc ccagcatctt
    31441 gacatgggcc agtgagcctt gaatatcata cttggccaat ttttggtcaa agcgaatgga
    31501 ggctccgaat tcttcaaccc attcctcaag actggcttca aagcgacctc cccataattt
    31561 ctttgcttcc ataagctcct actttctagc tctttttatg aatttccgca ttgaccttgg
    31621 ttggtaatcc ccaaagcttg atgaaaccaa ctgctgcatc ttgatcaaag gtatctgcac
    31681 tggtataggt tgccaaatct tcgtcataaa gggaatttgg agatttccga gcaaccactt
    31741 tggctgaccc cttatacaat tttacttttg cggtgccatt aacaactgcc tgggtctgag
    31801 caagataggc cttcaaggca tctgtcgcag ggttgaacca caggccattg taaatcaagt
    31861 tagagagttc atttgaaaca attggtttga aatgcgatac ttcccgcaca agggtcaaat
    31921 cttcgatttc cttgtgggct gtcagaaggg taatagcacc tggacactca taaatttccc
    31981 gtgacttgat tccgactaaa cgattttcca catggtcaat ccgacccaca ccatgtttac
    32041 cagcaatgac attgagtctt tgaatgagat tagcaagact taacttttca ccatttacag
    32101 caacaggaat accggcttta aactcaatat cgacatactc aggagtatct ggtgcatttt
    32161 ctggagaagt agtgatgcca aaagcatctt ctggcgcttc attccatgga ttttctaaaa
    32221 ctccgcactc attggcccgt ccccaaaggt tttggtcgac tgagtaagga ctgtccaggt
    32281 cagctgggac tggtacacca ttttccttgg cgaagatgat ttcttcttca cgcgcccact
    32341 tccattcacg gacaggagcc acgactttca agcttggatc tagggctgca atggctacct
    32401 caaaccgcac ctggtcattt cccttaccag tacagccgtg ggcgatagtg gttgctcctg
    32461 tcttatgagc catttccacc aactttttag aaatcaaggg acggctgaga gccgatacca
    32521 gcggatattt ttgctcatag taggcatggg cttgtagggc tggtaggaca tattcttgag
    32581 cgaattcttc ttttacatcc aatacatggg actcaatagc accgattgat aaggccttat
    32641 catgtatgaa gtcgagatcc ttgccctctc caacatccat acaaaccgca atgacatcat
    32701 agtctttctt caaccaagca atcgcaactg aagtatcaag tccaccagaa taggctaaaa
    32761 tcaatttttc ttttgtca
//
LOCUS       DAZQCJ010000012        32534 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000012 DAZQCJ010000000
VERSION     DAZQCJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32534
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.012"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(93..623)
                     /locus_tag="ACHU66_000517"
     CDS             complement(93..623)
                     /locus_tag="ACHU66_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR3388826.1"
                     /translation="MKQNQLRQLTEISLFAALICVVVFFIRIPFGSQFVHLGNALVVV
                     GVLIFGANKGALAASLGLGIFDLLSGYASVAWITILESLIVCFVLHLFYEKFLQANDS
                     LSNIMLVGIVAALTKIILNLLKYTLTGSLVGGLTPSASFLAALGRILGTYGTAITTII
                     AVPILYQVCKRLYKRK"
     gene            complement(586..1470)
                     /locus_tag="ACHU66_000518"
     CDS             complement(586..1470)
                     /locus_tag="ACHU66_000518"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HFR3388827.1"
                     /translation="MVQRLLLANDLPGVGKVALATSIPIAAACQVETMLLPTVLLSSH
                     TGGFPDVVVEDMTDLNQAYFEQWKNLNLELAGILSGYCRNPQQLSQLAMYAKQTETPL
                     IVDPVMGDGGKLYTGFTSTYVEAMKELIQSAKLILPNVTEAVFLTGRDYLGACYDRAA
                     IEDLLEALAEQVSADIVLTGISFDDSQIGVAYFQQETGEITTYMSKKYPANFFGTGDI
                     LSTLLAVATIQQINLHQAIPLALDFIDKSLECTLALDRDLKFGISFEPFLAELQQAFQ
                     TLKERNYETESIASINRN"
     gene            complement(1667..2356)
                     /locus_tag="ACHU66_000519"
     CDS             complement(1667..2356)
                     /locus_tag="ACHU66_000519"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR3388828.1"
                     /translation="MKFGIIAAMPQELKILVEHLQDATEIDVLGRTYYQGRIGQHEVV
                     LVQSGIGKVMSAMSVAVLADRFSVDVIVNTGSAGAVAEGIAIGDVVVANQLAYHDVDV
                     TAFGYAYGQMAGQELYYPADQVLLEQLRTVLAEQEMISHVGLIVTGDSFIAGQERIAT
                     IKTHFPEVLAVEMEGAAIAQAAVNTGKPFLVIRAMSDTAQGDANITFDEFIIQAGERS
                     AQTLIAFLEKN"
     gene            complement(2365..2685)
                     /gene="macP"
                     /locus_tag="ACHU66_000520"
     CDS             complement(2365..2685)
                     /gene="macP"
                     /locus_tag="ACHU66_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFR3388829.1"
                     /translation="MGKPLLTDEILEKVKKKQYSDQSSQFGHYRPNQDISMDDALFDP
                     YLEEDYQGYEEGQTIRIPVKPTVVKSRRIETIKREAFRSKVNKILFWVIILLIMFIIA
                     VLFI"
     gene            complement(2696..3241)
                     /locus_tag="ACHU66_000521"
     CDS             complement(2696..3241)
                     /locus_tag="ACHU66_000521"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFR3388830.1"
                     /translation="MNFEEKTIERTEIFKGHIFDVVVDDVALPNGGTGKRELIFHKGA
                     VCVLAVTPEGKMILVKQYRKAIERTIYEIPAGKLELGEEDTLEDAALRELEEETGYTS
                     DKLTLLADFYSAIGFCNERIRLYLADNLIKVENPRPMDEDEVIELHEVTLEEALNLVA
                     TGDICDAKTIMAIQYLQLMRK"
     gene            complement(3250..4632)
                     /gene="glmU"
                     /locus_tag="ACHU66_000522"
     CDS             complement(3250..4632)
                     /gene="glmU"
                     /locus_tag="ACHU66_000522"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR3388831.1"
                     /translation="MSNNYAIILAAGKGTRMKSDLPKVLHKVAGITMLEHVKRAVDAM
                     EPAKTVTIVGHKAELVQAVLEGQSEFALQSEQLGTGHAVMMAEPALAGLEGQTLVIAG
                     DTPLITGESLKNLINFHVSHKNVATILTAQADNPFGYGRIIRNADGEVQKIVEQKDAN
                     DFEKQVKEINTGTYLFDNKRLFEALKDINTDNAQGEYYLTDVISIFRQAGEKVGAYVL
                     RDFDESLGVNDRVALATAEAVMRKRINEKHMVNGVTFINPDATYIDIDVEIGAEAVIE
                     ANVVLKGQTVIGERTVLTNGTRVRDAKIAADVVISNSDIEESVIEEGVTVGPYAHIRP
                     GSLLKKDVHVGNFVEIKASTLGQGTKSGHLTYLGNATIGNNVNVGAGTITVNYDGKNK
                     FKTTVGDNAFVGSNSTIIAPVTIGDNALLAAGSVITKDIPEDAIGIGRGRQENKEGYA
                     TRFPFHPSQK"
     gene            complement(4809..5279)
                     /locus_tag="ACHU66_000523"
     CDS             complement(4809..5279)
                     /locus_tag="ACHU66_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFR3388832.1"
                     /translation="MTPTQLWQEFLAINSQAGPEPEAWAFGAEADRLADLVARGIKTS
                     TSSAHALYAVEGEEIPTAGGYDIILDGQGQAVCIIQTTKVYVTPFSQVTEEHAYKEGE
                     LRQGGDLSDIKAKSLIHWRQVHEELFTIWLAEAGLSFSEDMLVVCEEFELVYPK"
     gene            complement(5276..5827)
                     /locus_tag="ACHU66_000524"
     CDS             complement(5276..5827)
                     /locus_tag="ACHU66_000524"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR3388833.1"
                     /translation="MILETNRLLLRPWSETDAADLFSQASHHEVGPAAGWPVHQSVEE
                     SREIIKTVLSQPETYALVLKETGQVIGSIGLMLGKQGGLDLPDSEAEIGYWIGHSFWG
                     QGLVPEASQVLLDYGFSQLNLEKVWCRAFVENSKSLRVQEKLGFVYQYLLEDVHFPLI
                     DEVRSERVNLLPREEWQSRKEKL"
     gene            complement(5839..6537)
                     /locus_tag="ACHU66_000525"
     CDS             complement(5839..6537)
                     /locus_tag="ACHU66_000525"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFR3388834.1"
                     /translation="MTRTALITGVSSGIGLAQAEIFLENGWRVFGIDLASKPDLAGDF
                     HFLQLDLTGDLSPVFSWCQTVDVLCNTAGILDDYRPHLDIEEDELARIFAVNFFAVTR
                     LTRPYLQQMVDRQSGIIINMCSIASSLAGGGGSAYTASKHALAGFTKQLALDYAKDKV
                     QIFGIAPGAVQTGMTQKDFEPGGLADWVADQTPIGRWSQPSEIAELTFMLATGKLASM
                     QGQIITIDGGWSLK"
     gene            complement(6530..6766)
                     /locus_tag="ACHU66_000526"
     CDS             complement(6530..6766)
                     /locus_tag="ACHU66_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFR3388835.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTIEVHGQRLEAT
                     PYFLINVTGEGEGFSMWSPTPCDVLATDWVEVHD"
     gene            complement(6819..7613)
                     /locus_tag="ACHU66_000527"
     CDS             complement(6819..7613)
                     /locus_tag="ACHU66_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388836.1"
                     /translation="MSKQMEYRKQIEVIESQLTKENKEYMGRINGYMMIASVFHRQEE
                     AVTAQLLSIYQDVLEAQKDGLSAEDFLGKDSKQMADDLLSYLPPIGFMEVANLSGLML
                     IIYLGSQWLMDFAGTGTISLNWLGLICDAVLSLLLPSGIFLIIRGLIYQTSKIKVWAS
                     FLYIPLLFLVICGLRLWAMPKEPDLVLTGWELLVPLTLLGLALLFFQKEKLVRYVFMP
                     SYLFMIAGGVVNMVMTVPVWLNLVLLILPAMAFWIGSAILLVRKPK"
     gene            complement(7615..7932)
                     /locus_tag="ACHU66_000528"
     CDS             complement(7615..7932)
                     /locus_tag="ACHU66_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR3388837.1"
                     /translation="MKETQMLKGVLDGCVLQIISQKEIYGYELVQELRNQGFENMVGG
                     TVYPLLQKLEKNDLILSQNKPSPDGPDRKYFYLTDQGKAYLEDFWSQWTELVQKVQRL
                     KGE"
     gene            complement(7929..8093)
                     /locus_tag="ACHU66_000529"
     CDS             complement(7929..8093)
                     /locus_tag="ACHU66_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388838.1"
                     /translation="MKLDKKSRGNWKLAGITLVMAIITGWALDSILILGILLASGLMF
                     GFFTDDEEGL"
     gene            complement(8284..10296)
                     /locus_tag="ACHU66_000530"
     CDS             complement(8284..10296)
                     /locus_tag="ACHU66_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFR3388839.1"
                     /translation="MFGLTVRLALTNLKKNRRLYFPYALMTILSTAIAYIFASLAFHP
                     DLGQVKGADGVTMVLGFGMIVVMLAVAIMVFYANSFVMKQRSKEFGVYSILGLEKKHL
                     LLMTFLENLVFAASSILTGLLLGLALDKLFYALLLKVMQMPVVLASTFQLKSLVTVVI
                     YLFGIFALVSLFNIGKLGLTDSLKLVQGKKRGDKKTGFLWLQTLLALILLGAGYAIAQ
                     LVTSPMQAIPSFFGATLLVILGTYLLFHAGVISLLNWLKNRQTYYYKPDNFISVSNLI
                     FRMRKNALGLATITILSSMFLVTMVGGLNIYIGGKDYIANQNPNDYSIDVGMQPTTSK
                     AQMEKWEEWAEAILEETDIPVESKVVYPYQQAYFSEVTNQQVRFLTDEEAAGMNFSDR
                     VGIGFVLDQASYEKMTGEKLQLASDQVAIFSKGVQYQAGQTLTFDEKEMEVVQVLPKN
                     VTLGHLPDHGSFLLSQYLVIVVQDLAVFENQAENRYYMGFESSLSEEEQVDSHDTFLS
                     GSFESELWESNILPNGTNAISLAYRAYAMRGFFSFAGSLFFIGLLLSLIFLMAVVLVI
                     YFKQISEGYEDRDRFVIMTKVGLDEDQTRQSIRKQLLTVFFLPILLAFVHLAFAYKML
                     SSILLSIGVVNAGLMLQVTVGICGGYLLLYLVVYAITTRSYKQIIS"
     gene            complement(10298..11056)
                     /locus_tag="ACHU66_000531"
     CDS             complement(10298..11056)
                     /locus_tag="ACHU66_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3388840.1"
                     /translation="MPLLDVQHVQKIYSSRFKAGQVEALKDIHFTVEKGEYVAIMGES
                     GSGKSTLLNILATLDKPTQGKVLLNGLDTQSIKSKEAAVFRREKLGFVFQDFNLLDTL
                     SVKDNVLLPLVLSRYPTDEMNKRLVHTLNSLGIATLQDKMPYEISGGQQQRVAVARAI
                     ITQPEILLADEPTGALDSKSTATLLDIFEKINQEGQTILMVTHSTVAASRAKRVLFIK
                     DGILYNQIYRGERTSQEMYQLISDTLTLMANRGE"
     gene            complement(11154..12146)
                     /locus_tag="ACHU66_000532"
     CDS             complement(11154..12146)
                     /locus_tag="ACHU66_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR3388841.1"
                     /translation="MNKDDFGSLVPGFLTSWLGHRLIFLIAYAVFAVAILAYSSLFDI
                     KRNLVFYAILLLSICWLLALLWDFFREFSRFGKIWRGQKVATGTASERLLLEKVERLE
                     VQNKQILEKQRQEQTELDDYYTLWAHQMKTPIAASQLLVKEVDSSPVRHQLESELFKI
                     EQYTGLVLNYLRLQSFHDDLVIEAVNLEELVRSLVKKYSLFFIQANTSLDLGQLDRLV
                     KTDKRWLGLLIEQILSNALKYCQEGTVSIFLDGDELVIRDTGIGIAESDLERVFERGF
                     SGFNGRRTQQSSGLGLYLSRKIAAELGYSLKLQSKVGQGTEVRIGIKEVELIFD"
     gene            complement(12139..12822)
                     /locus_tag="ACHU66_000533"
     CDS             complement(12139..12822)
                     /locus_tag="ACHU66_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR3388842.1"
                     /translation="MQKILLIEDDKTISQLVAKNLINWGYQVQEVKDFQMVLEQMEEF
                     QPHLILLDIGLPFFNGYYWCQEIRKTSRVPIMFLSSHDQPMDIVMAINMGADDYVTKP
                     FEMTVLLAKIQGLLRRTYDFVGEQSLLWFEEISLDLKTMQVSYGQVVEELTRNEFQIL
                     RVLFEHGKEVVSREELMRELWNSDIFVDDNTLSVNIARLRKKLAELGLPDVIATKKGV
                     GYGLVWTHE"
     gene            12992..14161
                     /locus_tag="ACHU66_000534"
     CDS             12992..14161
                     /locus_tag="ACHU66_000534"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HFR3388843.1"
                     /translation="MPNLLTYIEETQYDNFYDKPINKLDILALTELSYLPFDNLVPYS
                     FTENGVRLDRLAATFEETYKNNFPPFSMVTKNRLALLGLLAKSIRFKSIKAFGFVDDY
                     QLEQEKQFSAISYRIDRKTIVTCFRGTDDTIIGWKEDFHMTYMDEIPAQRAASGYLEK
                     LMMQQGGHFYLAGHSKGGNLALYAACQQATDQQDRILAIYPFDSPGLHKKHLEAPSYQ
                     AIHQRIFPVIPQNSIVGMMLETPENVQIVQSNTLGILQHISFSWEVDGSDFKPAPALT
                     SDSLQTDQALKTWTASLTDDELRDFFDLFFGIFIKAGIERFSDITVNPLQKLQEIDRL
                     RKEIAPEEAEMVDKLIRLLFDTRYQIWRDNIPSPEISLPDWRKLFQRNTTENKEN"
     gene            complement(14176..15300)
                     /locus_tag="ACHU66_000535"
     CDS             complement(14176..15300)
                     /locus_tag="ACHU66_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFR3388844.1"
                     /translation="MKLPRFGVEEWLNVHENSAIYDIAGVSISSLTLEELFALSGTNP
                     EDFYKKLQGTKLNYGWIEGSPAFKKSVSQLYTGVKPEQILQTNGATGANLLVLYSLIE
                     PGDHVISLYPTYQQLYDIPKSLGAEVDLWQIEEENGWLPDLEKLRQLIRPTTKMICIN
                     NANNPTGAVMDRTYLEELVEIASEVGAYILSDEVYRSFSELDVPSIIEVYDKGIAVNS
                     LSKTYSLPGIRIGWVAANHQVTDILRDYRDYTMICAGVFDDLVAQLALAHYKEILERN
                     RHILEENLAILDQWIEEEPLVSYIRPAVVSTSFVKIAVDMPMEDFCLQLLQEHGVLLV
                     PGNRFERDGYVRLGFACEQETLIKGLEKLSQFLRRFDKEN"
     gene            complement(15448..16209)
                     /locus_tag="ACHU66_000536"
     CDS             complement(15448..16209)
                     /locus_tag="ACHU66_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HFR3388845.1"
                     /translation="MSHQLDNAKNLYLRGIRDGEIKEVHENYMGASYTQHSTGVPDEK
                     EGFAAFFEDFFKRNPKREITIARAIEDGNFVFVHVHQKLNDGAAEWVTADIFRSDENG
                     CIVEHWDVIDAYPKTIGQTDPIYADFELTDLDKTEDNKKIVRRFLVDVLQNGKIDKFD
                     DYVAANLIQHNQEIAQGGAAYKDYLVDKAVNYDFVFKVMGQGDYVVAYSKVWIAGQDY
                     AHFDIYRLKDGKIVEHWDNKEVMPDKKDLTNLGKF"
     gene            complement(16219..17730)
                     /locus_tag="ACHU66_000537"
     CDS             complement(16219..17730)
                     /locus_tag="ACHU66_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFR3388846.1"
                     /translation="MNLFETFIERKDEWGVALFEHLRISLLALIIAIAIAVPLGLILS
                     SKKRLTEWSLQITGIFQTIPSLALLGLFIPFMGIGTLPAVVALVIYAIFPILQGTLTG
                     LGEIDPSLEEAATAFGMNKWEKLKKFKLALAMPILMSGIRTASIMIIGTATLAALVGA
                     GGLGSFILLGIDRNDSALILIGAVSSAVLAVLFGYGIRLLQDKKPKTILLALLLTLFT
                     VGASYVPMLNFSTKQLVIAGKLGAEPEILINMYKLLIEDQTDIKVEIKPNFGKTSFLY
                     EALKSGSIDIYPEYTGTITSTLLKNSSMDLSTNSDEVYTYAKEAILEQDGLVYLAPMA
                     FQNTYALAVTEDYAQKNGIEKISDLAKVQQTAVAGFSLEFNDREDGNIGLKNLYNLQL
                     NVKTMEPALRYEAIKSGNVQIIEAYSTDSKVVTYKLKILKDDKRLFPPYQAAPLLSKE
                     TLEKYPELEQALGVLAGNISTEEMTRMNYAVDVEGKSAEQVAREYLEQEGLLK"
     gene            complement(17730..18461)
                     /locus_tag="ACHU66_000538"
     CDS             complement(17730..18461)
                     /locus_tag="ACHU66_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR3388847.1"
                     /translation="MIDYQNVSLTCKVSGPILKNLTFDIQEGEFFVLIGPSGSGKTTT
                     LKLINRLIEQTEGEVFFQGKRLKDFDLRELRLETGYVLQQIALFPNMTVAENIALIPE
                     MKGLGKEETLTRTRELLTKVGLEPDSYLDRLPKDLSGGEKQRVSILRAIIANPKVLLM
                     DEPFSALDPISKAQLQDLIKELHEEFKMTTVFVTHDMDEAVKLADRICLMQDGQVVQL
                     GSPDELRNHPANDFVKEFIRARGGM"
     gene            complement(18454..18903)
                     /locus_tag="ACHU66_000539"
     CDS             complement(18454..18903)
                     /locus_tag="ACHU66_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFR3388848.1"
                     /translation="MMEVEKRSKDYARMFDQQVGLYEDYARGHGLNAKCLSILMWIYY
                     NPGGVTQNWVSKKTYSSKQVVNATVKKFLDGGLVVLEENPADKRHKKIKLTEEGQQFA
                     SRILDPLEEAENKALSQLSQEEQELMLGLIQRYSQSLMAILKGEDDD"
     gene            complement(19214..19750)
                     /locus_tag="ACHU66_000540"
     CDS             complement(19214..19750)
                     /locus_tag="ACHU66_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFR3388849.1"
                     /translation="MVKKSSSVWTSLLLGAAGGAAAAAFLASKTGKTVKEKVVNFAND
                     YKENHEEINADFVNKAQDLGKQATERFTEVKTQLETGELTVEDLVKSGKEKSLETFEQ
                     IKEKIAEQNLSTADILEAIKAKTAKAPTVDLTEEDIEDAVVVSEDIELTIDDVIIEPV
                     SETVSEVASETASETVEG"
     gene            complement(19734..20144)
                     /locus_tag="ACHU66_000541"
     CDS             complement(19734..20144)
                     /locus_tag="ACHU66_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFR3388850.1"
                     /translation="MIEISVLIIALSIAAVAVYIVLLLKKLGTVTDEAQQTLKVLTSD
                     VNVTLYQTNELLAKTNVLVEDVNGKVSTLDPLFVAVADLSTSVSDLNASARDLTVKAK
                     SAGASTVKAGGALSALSTLSSLLGKKGDKNGKKI"
     gene            complement(20158..20934)
                     /gene="lgt"
                     /locus_tag="ACHU66_000542"
     CDS             complement(20158..20934)
                     /gene="lgt"
                     /locus_tag="ACHU66_000542"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR3388851.1"
                     /translation="MDPIAIKLGPLEIRWYAICILLGLILGVYLATKEGPRKKIRQDD
                     ILDFILIAFPLSILGARIYYVAFSWSEYKDNILSIFAIWNGGIAIYGGLITGAIVLYF
                     FTQYRFINTLDFLDIVAPSVMIAQAIGRWGNFFNQEAYGKAVESLNYLPAFIRDQMYI
                     DGAYRQPTFLFESLWNLLGFGLVCVLRRRPKFLKQGEITAFYLVWYGCGRLLIEGLRT
                     DSLMFLGIRVSQWLSGVLILVGIIMVVLRRRKSSIPFYQP"
     gene            complement(20936..21868)
                     /gene="hprK"
                     /locus_tag="ACHU66_000543"
     CDS             complement(20936..21868)
                     /gene="hprK"
                     /locus_tag="ACHU66_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFR3388852.1"
                     /translation="MTVYVKDLVDNLRIECAYSTEELLQKQITTADITRPGLEMTGYF
                     DYYAPERIQLIGMKEWSYLMAMTAHNRYQVLSQMFQPETPVVIVARGLEIPEEMYKAA
                     KEKQIAICRSKTATSRLSGELSSYLDSRLAQRTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDDVTLWGEPAEILRHLLEIRGVGIIDIMSLYGAS
                     AVKDSSEVQLAVYLENFETGKVFDRLGNSGDSIEIAGIAIPQIRIPVKTGRNISVVIE
                     AAAMNYRAKQMGFDATKIFEERLSNLIEHNREEV"
     gene            22083..23027
                     /gene="manA"
                     /locus_tag="ACHU66_000544"
     CDS             22083..23027
                     /gene="manA"
                     /locus_tag="ACHU66_000544"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFR3388853.1"
                     /translation="MMEPLFLTPVMHEKIWGGNRLRTNYHYDIPSEKTGECWAISAHP
                     NGVTTVSNGQYKGRGLDDLYKNEKHLFGNPTDDVFPLLTKILDADDWLSVQVHPDDSY
                     GLAHEGELGKTECWYILEAEDGAEIIYGHNAQSKEELRQQIEAGDWDKLLTHVPVKKG
                     DFFFVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRRDDAGNLRELHIEKSIDVLTI
                     GPVANSTPAHLKAGNLDSTLLVANPFFTVYKWNIQQEIKMEQTVPYLLVSVIEGEGAI
                     QVGETSYPLQKGSHFILPANVTDWKFTGQMNIIASHTN"
     gene            complement(23175..23447)
                     /locus_tag="ACHU66_000545"
     CDS             complement(23175..23447)
                     /locus_tag="ACHU66_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFR3388854.1"
                     /translation="MSIELYKVRQGKIVSGVLAGIAHKLGWESWVTRAIFIAGLLLGK
                     SFLLLIALYIAAAYFLPYKEDRDAERYGTGPRKIKEAEKINKSWFE"
     gene            complement(23437..24198)
                     /locus_tag="ACHU66_000546"
     CDS             complement(23437..24198)
                     /locus_tag="ACHU66_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388855.1"
                     /translation="MTQKREERLIELYEKGVLTKEEARACFKEMNQEPDFLFVEEKVK
                     LNFTLPSFKVFASSKLKQSYSFEGIESLFLKLSEGRLTLAKSKNNQISVEICYNQDAP
                     EDKLPRLYVEKKGLYFHSSLACRLTINLPQEWMSVLDLELGQADARLDYLPFEDISIR
                     SSRDKKQQDIRLTTCGGYPQHLYVQLAQAPLTLQTGKGQGIRGQIESQSGHVLVNRKK
                     KNSPYQFEKSGNDLLFLKAQTGQGGFYVKGIKDVN"
     gene            complement(24268..24702)
                     /locus_tag="ACHU66_000547"
     CDS             complement(24268..24702)
                     /locus_tag="ACHU66_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFR3388856.1"
                     /translation="MDLNKYVQEVSLQDFGKEFRHVAIWNRRLRSTGGRFFPRDGHLD
                     FNPKHLEEQGLEVFRKIVRHELCHYHLYFEKKGYRHGDKDFKELLAAVDGLRYAPKLE
                     QVAKPSLLYTCQSCGQVYQRKRRIDLKKYRCGKCRGKLTLKE"
     gene            complement(24689..26845)
                     /locus_tag="ACHU66_000548"
     CDS             complement(24689..26845)
                     /locus_tag="ACHU66_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR3388857.1"
                     /translation="MEEKYMKIAQELGVSLKQIDTVLSLTAEGNTIPFIARYRKDVTG
                     NLDEVVIKSIIDRDKALTALADRKATVLAKIEEQGKLTDQLRQAIEEAEKLADVEELY
                     LPYKEKRRTKATVAREAGLFPLARLILQNVADLEEQAADFICEGFDTAQSCLAGAVDI
                     LVEAISEDNKLRAWVYHEVQTNSNLTSELKDQEADEKEVFQIYYDFSEKVAKMQGYKT
                     LAINRGEKLGVLKVSFEHNVDKMIRFFELRFPQSNSYIKDVIQQAIKKKILPAMERRI
                     RTELTEEAEEGAIQLFSKNLRNLLLVSPLKGKVVLGFDPAFRTGAKLAVVDQTGKLLT
                     TQVIYPVEPAGQRQIAQAKKDLADLIGQYQVEIIAIGNGTASRESEAFVADLLKDFPD
                     VSYVIVNESGASVYSASELARYEFPDLPVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVNQKSLSESLDFVVDTVVNQVGVNVNTASPALLAHVAGLNKTISENIVKYRE
                     ENGALTSRQQLKKVPRLGDKAFEQAAGFLRIPDATNFLDNTGVHPESYKAVENLLELL
                     AIDHLDEAAQEKLKQVAIADTAEKIGVGQETLKDIVADLLKPGRDLRDDFEAPVLRQD
                     VLDVKDLVVGQELQGTVRNIVDFGAFVDIGVHEDGLVHISRMVKRNRDKNGRQQALPH
                     PSEVLAVGEIVTVWVVEVDIKRNRIGLSLLKPNGSE"
     gene            complement(26906..27757)
                     /locus_tag="ACHU66_000549"
     CDS             complement(26906..27757)
                     /locus_tag="ACHU66_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFR3388858.1"
                     /translation="MKQRAGIRYIFLILVGILILLYPYISSSWNRYRDQQLISSHREW
                     VDTQPEQEKEKMWQVAQSYNNELGIQPVPDAFSLREGIHDPKYETVLNASQDGMMGYV
                     EIPKIDIELPIYHYTSDSVLQKGAGHLFGSALPVGGDGSHTVVSAHRGLPNAEMFTNL
                     PQLEKGDQFYFHVLGEILAYEVDWIQVVKPNQVSSLSGIEGEDYATLITCTPYGINTE
                     RILVRGKRVPYDKKDYEKAQSSQKKVDEIYLAVQVVCGVLGFLLSLLIILFVKKRDQK
                     RKNKESM"
     gene            complement(27825..29465)
                     /locus_tag="ACHU66_000550"
     CDS             complement(27825..29465)
                     /locus_tag="ACHU66_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFR3388859.1"
                     /translation="MKRIFTLLTVLLTVLGMNARPVFAASNTDQAELKITNIEGNPTV
                     TLYKIGEGVYNATNDSFIRFDYASGVKLTETGPTAEEITNIANGILDNTIQAKASKVL
                     QVTNGTATYRATGAGVYIAILTGATDGRSFNPILLAASYNGEGKLTGGSVDSKAKYLY
                     GQTSVAKSTLPSIDKTVSGVTADADKNSASLGQVLTYSLNVTLPSYPTQAKNKTIFIS
                     DTMSEGLTFDYASLTISWNGQTLIADTTGQFKTQGDILIAKAVKVGNGFNLAFVYDNL
                     NEIAPVVTYKGIINDKAVVGGTGNANKAEFFYARNPNSGNTYEDPNNKPNPQNDNTIV
                     NKEDSETVYTYQIAFKKVDSITKAPLAGAVFGIYSDAETKQLVDIVTTNADGYAISTK
                     VGKGTYYLKEITAPTGYSLNTNVYTAEASWTSATTTSSASSTRSEYTSVVGEAKDSTQ
                     VGWLKHNVFYKLDNKPEGTDVQAAYLKATTTTAQNTTVTTTNPTAGAGTVSIADVPNT
                     KLGELPSTGGMGTYLFTFIGVLVLTVGLGLYFTKNKKA"
     gene            complement(29492..32476)
                     /locus_tag="ACHU66_000551"
     CDS             complement(29492..32476)
                     /locus_tag="ACHU66_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFR3388860.1"
                     /translation="MFGKRWLSIFAILLLTLSNCSPTIGYALENMSESTSSSVISSAE
                     ETVSKSETTTEMSQSSEEIVEMKQAEENTETNRSDAPSSSQDLADNSEESVQKILHYE
                     DEYVTADLNAYFGNGVENFSVTLPRMKNSGKWDSEREYVNQLSQYLVNQNLQPADVQQ
                     IEISDSQIVKEGYKPRLTVNFKTSLGLRGGLVTKENLRLVSTNTDGSLVFHDAEFHFD
                     TTGTLIGLTYEGILGKDIHVVMVKPLAEKTTVTLGQYVQMQLDGYVLEEEEEQTASNV
                     PLNARLKVKIFRSDASGIFTILSPIDSFREEKTFDVLSTWKPSFNLLGIESEKFDIVK
                     MNYRQAHQSKTDSLMYFNGEFPVASFHQESGEEESNVLEIYLQNRMLEGTGMILGLRT
                     RMLGYRSFSGFRSVTNATVSNSSVDQAHITQSKRIDYLGDGGNNPDTSVDDRIGTDTH
                     ELYRMYLDMTGTKEPFDTLIVVDRSTSMTDPMNSVDTQARYLAVYKALNGTAGRQGLL
                     SKLVGFHPENQVAIVGFQGYPGYPSGDQDSTVIANWGRSTSVALSNIQPPYNNGTNYT
                     AGLRTAGVVLDQNQSSRKKVMIFISDGVPTFAFVNGVRYGNGTISGNNPYYTRDWTLN
                     YFNSWIGKYPKLPIYTLGISSEFGNSDNLSANPYVLNHMSSQTGGFYSHVADSQALER
                     TLQKIVDDTKLSLVSINDQLSQYVSYYAEQPDVKVVQTNRQTGQVTTLYQNGAVTSQG
                     VGILQSVTHTAVNASDSTGKVDLNFVDNFKIDDTYTYTISYNVKVTDRAYEEKKTSGY
                     NAHGDSGTDYTGNSTSSGQAGFRSNKVASLTYIRNEQPYEKEYDHPVVQVDTRQFTLQ
                     KSDSKETNLLLENVRFELRLSDKKTVFASGETDSRGNITFTDLRRAKTYYLYETKAKD
                     GYTLPEDPWTVDVAVDGTITIRDQLGYTVSKSGEQYRVVNHKLYQLPSTGGYGPAVYI
                     LLGVMVITLASYLFYEKRKQIA"
BASE COUNT         9313 a         7723 c         6160 g         9338 t
ORIGIN      
        1 agagtataac ctcctgtttg tttcatcact tacagtgtag tccttctgac ctttttttgc
       61 atattgaatt taactaatgt cactaatata aattactttc tcttatataa ccttttacat
      121 acctgataaa gaataggtac agcaatgatg gtcgtaattg cagtgccata ggttcctaaa
      181 atcctaccca aagcagctaa aaatgatgct gatggcgtta gtccaccgac taaactaccc
      241 gtcaatgtgt acttcaacag gttcaagata atttttgtga gggctgcaac tatcccaacc
      301 aacataatat ttgatagact atcgtttgcc tgtaggaatt tctcataaaa taggtggagg
      361 acaaaacaga caatgagtga ttctagaatg gtaatccaag caacggaagc atatcccgac
      421 agtaaatcga agatgcctag accgagtgag gctgccagcg cccctttgtt ggcaccaaag
      481 attagtactc ctactaccac cagagcattg cctagatgga caaattgact cccaaaagga
      541 atacgaataa agaagacaac cacacaaata agggctgcaa ataggctaat ttctgttaat
      601 tgacgcaatt gattctgttt cataatttct ctcctttaat gtttgaaatg cctgctgtaa
      661 ttctgctaga aaaggttcaa aggaaatccc aaatttcaag tctctatcca aagcaagagt
      721 acactctaaa ctcttgtcga taaaatctaa agctagaggg atagcttgat ggagattgat
      781 ttgttggatg gttgctactg ctaacaaggt agatagaatg tcacctgttc caaagaaatt
      841 tgctggatat tttttagaca tataggtagt tatttcccct gtttcctgct gaaaataggc
      901 tacacctatt tggctatcat caaaggaaat ccctgtcagt acaatatctg cggatacctg
      961 ctctgctaaa gcttccagta agtcctcaat agctgcccta tcgtagcaag ctcccaaata
     1021 atccctccct gtcaaaaaaa ctgcctctgt tacattgggg agaatgagtt tcgccgactg
     1081 tatcaactct ttcatggctt ctacataggt agatgtgaaa cctgtataca acttccctcc
     1141 atcccccatg accggatcaa caatcaatgg tgtctctgtt tgtttcgcat acatagccaa
     1201 ttgacttagt tgctgaggat ttctacagta gccagacaag atgcctgcca actccaaatt
     1261 caagttcttc cactgttcaa aataggcttg attcaagtca gtcatatctt caacgacaac
     1321 atctggaaaa cctcctgtgt gtgaggataa aagaactgta ggtaaaagca ttgtttctac
     1381 ctgacaagca gccgcaattg ggatgctagt ggctagggca accttaccca caccaggcaa
     1441 atcatttgca agcaataatc gctgaaccat aacaacctcc taaattattt ttcttaaaca
     1501 aaattgtaaa gtattttcac gagcttttca ataactctta tcagaaattg tatggggaca
     1561 gatggctgaa accttacaac accagtctca aatcactgca taagaaaaac agtaacaact
     1621 ttttgctact gtcatcctat ttcaatgtat tgttgtcaaa atctgcttaa tttttctcca
     1681 ggaaggcaat cagggtctgg gcagaacgtt ctcctgcttg aatgataaat tcatcaaagg
     1741 tgatattggc atctccctga gctgtgtcac tcatggcacg gataaccaag aatggcttac
     1801 ctgtattaac agctgcttga gcgatagctg ctccttccat ctctacggca aggacttctg
     1861 gaaaatgagt tttgattgta gcgattctct cctgacctgc gataaaactg tcacctgtca
     1921 caatcagacc cacatgacta atcatttcct gctctgctaa aacggttctt agctgttcta
     1981 acaagacttg gtctgcagga taatacaatt cctgccctgc catttgcccg taagcatagc
     2041 cgaaagccgt cacatccaca tcgtggtatg ccaattgatt tgctacaacc acatcaccga
     2101 tggcaatacc ttcggcaaca gctcctgccg agcctgtatt gacaatgaca tcaaccgaaa
     2161 aacgatctgc caaaacagcc acggacatag ctgacataac cttaccgata cctgactgaa
     2221 caagaacgac ttcatgttga ccgattcgtc cctgatagta agttcgaccc aagacatcga
     2281 tttcagttgc atcttgtaga tgctcgacca aaatctttaa ttcctgaggc atggctgcga
     2341 taattccaaa tttcatctct tctcctaaat aaataatact gcaatgataa acattatcag
     2401 taagatgatg acccaaaaaa ggattttgtt taccttactg cggaaagctt ctcgcttgat
     2461 ggtttcgata cggcgacttt ttactacagt aggtttgacc gggattcgga ttgtttgccc
     2521 ctcttcatat ccctgataat cttcttctag atatggatcg aatagagcat catccattga
     2581 tatatcctgg tttggacgat agtgtccaaa ctggctagat tggtcggaat actgtttctt
     2641 tttcactttc tctaaaattt catccgtcaa caagggcttg cccattgcta gtttcctact
     2701 ttctcatgag ttgtaaatat tggatagcca tgatggtctt tgcatcacaa atatcgccag
     2761 tagccaccaa gttcaaagcc tcttccaagg ttacctcatg taattcgata acctcatctt
     2821 catccattgg acgaggattt tcaaccttaa taaggttatc tgctaaataa aggcgaattc
     2881 gttcattaca gaaaccgata gctgaataga aatctgccaa caaggtcagt ttatcactag
     2941 tatagcctgt ttcttcttct aattcacgca aagcagcatc ttcaagcgta tcttcttcgc
     3001 ccagctccaa tttccccgcc ggaatttcat aaatagtacg ttcaatggcc ttgcggtatt
     3061 gtttgaccaa aatcatttta ccttctgggg taacggctaa aacgcaaact gctcccttgt
     3121 ggaaaatgag ttcgcgctta ccagttccac catttggcaa ggcaacatca tctactacta
     3181 catcaaaaat gtgcccttta aaaatttctg tccgctcaat ggttttttct tcaaaattca
     3241 tatgattacc tatttttgac ttggatggaa cggaaaacga gtcgcataac cttctttgtt
     3301 ttcttgacga ccacgaccaa taccgatggc atcctctgga atatcttttg taatgacaga
     3361 acctgcagcc aacaaggcgt tgtccccaat agttactggt gcgatgatgg ttgagttaga
     3421 gccaacaaag gcattatctc caacggttgt tttaaattta tttttaccgt cgtagttgac
     3481 agtaatcgta ccagcaccaa cgttaacatt attgccaatc gttgcatttc ctaggtaggt
     3541 caaatgacca gacttggttc cttgaccaag tgtagaggcc ttgatttcca caaagtttcc
     3601 aacgtgaaca tcttttttca agagactacc tggacgaata tgggcgtatg gtcctaccgt
     3661 cacaccttct tcaatgacag attcttcaat atctgagttg ctgatgacga catctgctgc
     3721 aattttcgca tcacgtacac gagtcccatt ggttaaaaca gtacgttcac caatcacagt
     3781 ttgccctttc aagacaacat tagcttcgat aacagcttct gcaccaatct caacatcaat
     3841 atcaatatag gttgcatctg ggttgataaa tgtcacacca ttgaccatgt gtttttcatt
     3901 gattcgtttg cgcataacag cttctgcagt cgcaagagcg acgcgatcat tgacacccaa
     3961 actttcatca aaatcacgca agacataagc accaaccttc tcgcctgcct gacggaaaat
     4021 cgaaataaca tctgtcagat agtactcacc ttgggcattg tccgtattaa tatctttaag
     4081 cgcttcaaaa agacgtttat tatcaaacaa gtaggttcct gtattgattt ctttcacttg
     4141 tttttcaaaa tcgttggcat ctttttgctc aacaattttc tgcacttccc catcagcatt
     4201 gcgaataatc cgaccatatc cgaatgggtt atcagcctga gctgtcaaga tagtcgcaac
     4261 attcttgtgg ctgacatgga agttaatgag gttcttcaag ctttctcctg taatcagcgg
     4321 tgtatctcct gcgataacaa gggtctgacc ttccaaacct gcaagagctg gctcagccat
     4381 cataacggcg tggcctgtcc ccaactgttc agattgaagg gcgaactctg actgaccttc
     4441 gagaactgct tggaccaatt ctgccttgtg accgacaatc gttactgttt tagcaggttc
     4501 catagcatct acagcccgtt tgacatgctc caacatggta attcctgcca ctttgtgtaa
     4561 gacctttggc aagtcagact tcatgcgagt tcccttaccc gccgctagga taattgcata
     4621 gttattgctc atagttttct ctcttttcaa tgatatactc ctattatagc aaattttgat
     4681 gtttttggaa aatagcagta ttatagggaa ttttcgtaaa taaaaaagaa actggattga
     4741 agccagtttc tcaaaaattt ctagtattag aaaacaaact acaggttgat tgcatccaat
     4801 agaattgtct atttaggata aaccaattca aattcttcac agacaaccag catatcttct
     4861 gagaaggaca ggcctgcttc tgccagccag atagtgaaaa gctcttcatg gacctgccgc
     4921 cagtgaatca gacttttcgc ttttatgtca gacaagtcac ctccctgacg gagctcgccc
     4981 tccttatagg catgttcttc cgtcacttgg gaaaagggcg tcacatagac cttggtggtc
     5041 tggataatgc acacagcctg accttgtccg tccagaatga tgtcatagcc accagctgtt
     5101 gggatttctt ctccctctac tgcgtagagt gcatgggcag agctggtcga agttttgatg
     5161 cctctagcca ccaagtcagc cagtcgatca gcttccgccc caaaggccca ggcctctggc
     5221 tcaggaccag cttgggaatt tattgcaaga aattcttgcc atagttgtgt aggggtcata
     5281 gtttttcctt cctagactgc cattcctctc ttggcagcag attcactcgt tcgctacgga
     5341 cctcatcaat gagtgggaaa tgcacatctt ccaagaggta ttggtaaaca aagcccagtt
     5401 tttcttggac acgtaatgat ttgctatttt ctacaaaagc acgacaccag actttctcta
     5461 aattcaattg ggaaaagccg taatccagta aaacttgact agcctctggc accaggcctt
     5521 gtccccaaaa tgaatgacca atccaataac caatctcggc ctctgaatca ggtaaatcaa
     5581 gaccaccttg tttaccaagc atgagcccga tactgccaat cacctgtcct gtttctttga
     5641 gaacaagggc ataggtttca ggttggctaa ggactgtctt gataatttct cgactttctt
     5701 ccacgctctg atgaacaggc caacccgctg caggaccaac ttcatgatga ctggcctgag
     5761 aaaataagtc agctgcgtct gtttcactcc aagggcggag caagagacga ttggtttcaa
     5821 gaatcataat attcctttct acttcaaact ccagccgccg tcgatggtga taatctggcc
     5881 ttgcatagag gcgagtttgc cagtagccag catgaaggtc agctctgcta tttcgctggg
     5941 ctgactccag cgtccgatag gggtctggtc tgccacccag tccgctaagc cgccaggctc
     6001 aaagtccttc tgggtcatgc ctgtctgaac ggcaccaggg gcgatgccga agatctggac
     6061 cttgtccttg gcatagtcca gagccagctg cttggtaaag cctgccaaag cgtgcttgga
     6121 ggcggtatag gctgaacctc ctccacctgc tagactggag gctatagagc acatattgat
     6181 aatgatgccc gactgtctgt ctaccatttg ctgcagatag gggcgagtta gtctagtcac
     6241 tgcgaaaaaa ttcaccgcga agatgcgagc taattcatcc tcctcaatat ccaggtgggg
     6301 acggtagtcg tctagaatgc ctgcggtgtt acagaggaca tcaactgtct ggcaccatga
     6361 aaagacgggt gataaatcgc ctgtcaggtc cagctgtaga aagtggaaat cgcctgccaa
     6421 atcaggcttg ctagccagat caatcccgaa gacacgccag ccattttcca aaaaaatctc
     6481 cgcctgagct agtccaatgc cgctggaaac tcctgtgatg agggctgtcc tagtcatgga
     6541 cttctaccca gtcggtggca aggacgtcgc aaggggtcgg gctccacatg gagaagccct
     6601 caccctcgcc ggtcacgttg atgaggaaat agggcgtcgc ctccagcctt tgcccgtgga
     6661 cctcgatggt gtcaaagagc tggacgtagt tctccgcccc gccccagcct gtgcgaacgt
     6721 actttttctt ggccttcaag cctggtaaaa tctcttcaaa tgtcatggtt tactccttta
     6781 ttcttctatc tttcaaccat tataccaaaa aatcgcttct atttcggttt tctcaccaac
     6841 aaaatcgcac ttccaatcca aaaggccata gctggcagaa tgagcaaaac cagattgagc
     6901 caaactggaa cggtcatgac catattcacc acaccgccag caatcatgaa cagataacta
     6961 ggcataaaga catagcggac caacttttcc ttttggaaaa atagcagagc gagaccgagc
     7021 aaggtgagtg gtactagaag ctcccagcca gtcaagacta agtcaggttc cttgggcatt
     7081 gcccaaagac gaaggccaca aattacgaga aaaagaaggg gaatgtagag aaaacttgcc
     7141 caaaccttga ttttgctggt ctggtaaatc agaccgcgaa taatgaggaa aatccccgag
     7201 gggaggagga ggctaagtac cgcgtcgcag atcaagccca gccagttgag cgaaatggtg
     7261 cctgtacctg caaaatccat cagccattgg ctgccgaggt agatgatcag catgaggcca
     7321 ctcaggtttg cgacttccat gaaaccaatc ggagggaggt agctgagtag gtcgtccgcc
     7381 atctgcttgc tgtctttgcc cagaaaatcc tccgcagaaa gcccgtcttt ttgagcttca
     7441 agaacatctt ggtaaatgga taagagctgg gcagtcactg cttcttcctg cctgtgaaaa
     7501 acagaggcaa tcatcatata accattgata cggcccatgt attccttgtt ttccttggtc
     7561 agctgagatt caatcacttc aatctgcttg cggtattcca tttgtttgct catcttattc
     7621 tcctttcaat cgttgaacct tttggaccaa ttccgtccac tggctccaaa aatcttctag
     7681 ataagctttt ccctggtctg tcagatagaa atacttcctg tctggtccgt ctggcgaggg
     7741 cttattctga ctaagaatca ggtcattttt ttctaacttc tgaaggagtg gataaaccgt
     7801 ccctccgacc atgttttcaa agccctgatt tctcaactcc tgaaccaatt cataaccata
     7861 aatctccttc tggctgataa tttgtaatac acagccatct aggaccccct tgagcatttg
     7921 agtttctttc ataagccctc ctcatcatct gtaaaaaatc caaacatcaa gcctgacgct
     7981 aacaggatgc ctaagatcaa aatagaatcc aaagcccagc cagttataat ggccatcaca
     8041 agcgtgatgc ctgctagttt ccaatttccc cgacttttct tatccagttt catactgcct
     8101 ccctacctag tttgtaatac agactagttt gataaaaata aaagtgattt tctttgtctt
     8161 ctatcactag tttgttttac ctattagctt tgtatctata gtataacaca aggcggctag
     8221 tatgtaaaac aaaatagtga aaaatattca aaaaaaaaat aaaaaacttg ggttgcccca
     8281 agtttaggaa ataatttgtt tgtaagaccg tgtcgtgatg gcatatacca ctaggtaaag
     8341 gaggagatag ccaccacaga tgcctaccgt tacttgcagc ataagccctg cattgaccac
     8401 accaatagac aacaagatag acgagagcat cttgtaggca aatgcaagat gaacaaaggc
     8461 aagtaggata ggtaggaaaa agacggtcag caattgcttg cgaatggact gtctcgtctg
     8521 atcctcatcc agaccaacct tggtcataat gacgaagcga tctctgtctt catagccttc
     8581 tgaaatctgt ttgaagtaaa tcaccagcac caccgccatc aaaaagatga gtgatagcaa
     8641 gaggccgatg aagaaaagtg aacctgcaaa actgaagaaa ccacgcatcg cataggcacg
     8701 ataggcgaga ctgatggcgt tcgttccatt cggtaatata ttggactccc aaagctcact
     8761 ttcaaaactt cctgataaaa aggtgtcatg gctatctacc tgctcttctt ctgatagact
     8821 gctttcaaat cccatataat agcgattttc ggcttgattt tcaaaaacag ccaagtcttg
     8881 aacaacaatg accagatact ggctgagaag gaaggaacca tggtctggta gatgtcctaa
     8941 agtcacattc ttgggcaaga cttggacaac ttccatctcc ttttcatcaa aggtgagggt
     9001 ttgaccagct tgatactgga ctcctttgct aaaaattgcc acttggtcag atgccaactg
     9061 gagtttttct cccgtcatct tttcatagct ggcttgatca aggacaaaac cgatcccaac
     9121 tcggtcagag aaattcatac cagcggcttc ttcatccgtc aaaaatctta cttgctgatt
     9181 tgtcacctca gaaaaatagg cttgctgata aggatagacc accttacttt caacaggaat
     9241 atctgtctct tctaagatag cttccgccca ctcctcccac ttctccattt gagcttttga
     9301 agtggtgggc tgcataccaa catcaatcga atagtcattt ggattttgat tggcaatata
     9361 atctttgcca ccgatgtaaa tattgagtcc tccaaccatg gtcaccaaga acatactgga
     9421 gaggatggta atggtcgcaa gccctagagc atttttccgc atgcggaaaa tcaagttgga
     9481 tacagaaatg aaattatctg gcttataata ataggtctga cggttcttta gccagtttaa
     9541 gagactgata acacctgcat ggaagagcag gtaggtccca aggataacca gaagagtcgc
     9601 tccgaagaaa cttggaatag cctgcatcgg actggtcacc agctgggcaa tggcgtagcc
     9661 tgcccccaat aagataagag ctaagagggt ttgtagccag aggaagcccg ttttcttgtc
     9721 ccctctcttc ttaccttgaa ccagtttgag tgagtcggtc aagcccaact tcccaatatt
     9781 aaaaaggctg accaaggcaa aaatcccaaa gagatagatg actacagtta ccagactttt
     9841 caactggaaa gttgaagcca agaccacagg catctgcatg accttgagca agagggcata
     9901 gaagagttta tctagagcca agcccagcaa caatcctgtc aaaatactac tggctgcaaa
     9961 aactagattt tctagaaaag tcatcaggag gaggtgtttt ttctccaatc ctagaatact
    10021 atagacacca aactcttttg accgctgctt catgacaaaa ctattggcat agaagaccat
    10081 gatggcaacc gccaacatga ccacaatcat accaaagccc aataccatgg ttaccccatc
    10141 ggctcccttg acctgaccta aatcaggatg aaaggcaagc gaggcaaaaa tataggcaat
    10201 ggctgtggac aaaatggtca tcagggcata gggaaaatag agtctgcggt tctttttgag
    10261 attggtcaga gccaatcgaa cggttagtcc aaacatacta ctcacctcga ttcgccataa
    10321 gtgtcaaggt atcggaaatc aactgataca tttcctgact ggttctctca cctcggtaga
    10381 tttgattgta gaggatgccg tccttgatga agagaacccg tttggcacga ctagctgcta
    10441 ctgtcgagtg ggttaccatc aaaatggttt gcccctcttg gttgattttt tcaaaaatat
    10501 ctagaagagt tgccgttgat ttggagtcga gggccccagt cggctcatcc gctaagagaa
    10561 tttctggctg ggtaatgatg gcacgcgcca ccgcaactcg ctgctgctga ccacccgaaa
    10621 tctcataggg catcttgtct tgcaaggtcg caattcccag gctattaagc gtatgaacga
    10681 gtcgtttgtt catctcatca gtcggatagc gagataaaac cagaggcaag agcacattat
    10741 ccttgactga gagggtgtcc aagagattga aatcttggaa gacaaagccc aatttctccc
    10801 gacgaaagac tgccgcttcc ttgctcttga tagactgggt atccaatccg ttaagcaaga
    10861 ccttgccctg cgttggctta tcaagggttg ccaaaatatt gagcaaggtg gacttaccag
    10921 aacccgactc acccataatg gcgacatatt cacccttttc aactgtaaaa tgaatatcct
    10981 tcaaggcctc aacctgaccc gccttaaagc gagaactata tattttttga acatgttgta
    11041 catctaagag tggcatcact gcaacctcct tttctttttc tacctccatt ctatcccatc
    11101 cgccacccct ctgccatagc cctagctttc atttgcgtga cagttttgta agattagtca
    11161 aaaatcaact ccacttcttt gataccgatt cgcacctctg ttccctgacc cacttttgat
    11221 tgtagcttta gggaatagcc cagctccgca gcaattttcc gtgagagata gagccccaga
    11281 cccgaagatt gctgggttct gcggccattg aaacctgaga aaccacgctc aaagacccgt
    11341 tccaagtcgc tctcagcaat cccaattccc gtatcgcgaa tgaccaactc atccccatct
    11401 agaaaaatcg aaacagtccc ttcctgacaa tacttgaggg cattggataa aatttgttct
    11461 atcaagagac ccagccagcg tttgtcggtc ttaacaagcc tatccaactg ccccaagtcc
    11521 aagctagtat tagcctgaat gaaaaatagg gaatatttct tgaccagact tctgaccaac
    11581 tcttccaaat tgacggcttc gataaccaaa tcatcatgaa aggactgtag acggagatag
    11641 ttcaagacca agcctgtgta ttgttctatc ttgaacaatt ccgattctaa ttgatgacga
    11701 actggactac tatctacttc cttgaccaaa agttgactgg ctgcaatcgg cgttttcatc
    11761 tggtgggccc agagggtgta gtagtcatct aattctgtct gttcctgtcg ctgtttttct
    11821 aggatttgct tgttctggac ttctagtcgc tctaccttct ccagtaacaa acgctcactg
    11881 gctgtcccag tagccacttt ttgaccacgc cagatttttc caaagcggga aaattccctg
    11941 aaaaaatccc agaggagggc gaggagccaa caaatactga ggaggaggat ggcatagaaa
    12001 accaagtttc ttttgatgtc aaagaggcta ctataagcca gaatagccac agcaaaaacg
    12061 gcgtaggcta tcagaaaaat caagcgatga ccaagccatg aggtcaaaaa tcctggtact
    12121 aatgagccaa aatcatcctt attcatgtgt ccacaccagg ccgtagccta ctcctttctt
    12181 ggtcgcaatc acatctggca gacccagttc tgccaatttc ttacgcaggc gagcgatgtt
    12241 aaccgacagg gtattgtcat ccacaaaaat atcactgttc cagagttctc tcatcagctc
    12301 ctcacggctg accacttcct tgccatgttc aaataaaacg cgtaaaatct gaaattcatt
    12361 tcgcgtcagc tcttccacaa cctgtccata ggacacctgc atggtcttta ggtctaggga
    12421 aatctcctca aaccagagta aactttgttc tccgacaaag tcataggttc ttctgagaag
    12481 cccctgtatt ttagccagga gaaccgtcat ttcaaagggc ttggtcacat agtcatccgc
    12541 ccccatattg atcgccataa caatatccat tggctggtca tgggaagaaa gaaacataat
    12601 gggcacacgg gaggtcttgc gaatttcctg acaccagtaa taaccattga aaaacggcag
    12661 gccaatatcc agtaaaatca aatggggctg aaactcttcc atctgttcca aaaccatctg
    12721 aaaatccttg acctcttgca cctggtaacc ccagtttatc aagtttttag caactagttg
    12781 gctaatcgtt ttatcatcct cgataagaag aattttctgc ataactatct cctttcatat
    12841 cactactatt tattatagct tacattggaa caaatttgag tatttttata catttacaca
    12901 agaagtagac tataaaacca ggtcatcagt ggtgcagatt tgtcagacca atcatatttc
    12961 gttatactag ataaaaaagg aaaggactgc tatgccgaat ttattaacct atattgaaga
    13021 aactcaatac gataattttt atgataaacc tatcaataag ctagatattc tggctctgac
    13081 cgaactttcc tacctccctt ttgataatct ggtcccctac tcttttacag aaaatggtgt
    13141 acgacttgat cggttggcag cgacctttga agagacctac aaaaataatt tccctccctt
    13201 ttctatggtg accaaaaacc gcctagcctt actaggactt ctagccaaat ccatacgctt
    13261 caaatctatc aaggcatttg gcttcgttga cgattatcag ttggaacagg aaaagcagtt
    13321 ttcagcaatt agttatcgca ttgaccgaaa aacgattgtg acttgcttca gagggacaga
    13381 tgatactatt atcggttgga aggaagattt ccacatgacc tatatggatg aaattcctgc
    13441 ccaacgtgca gcgagtggct acttagaaaa acttatgatg cagcaggggg gccattttta
    13501 tctggccggt cattcgaaag gcggaaattt agccctctat gcagcatgcc agcaagctac
    13561 agaccaacag gatagaatcc tagctatcta tccctttgat tcacctggct tacacaaaaa
    13621 acacttggaa gcacctagtt atcaagctat tcaccaacgg attttccctg tcattcccca
    13681 gaattcaatc gtcggtatga tgttagaaac accagaaaat gttcagattg tccagagtaa
    13741 tacccttggc attctacaac atatcagttt ttcttgggag gttgacggaa gcgacttcaa
    13801 accagcccct gccctgacca gcgatagcct acaaacagac caagccttaa aaacttggac
    13861 ggctagtcta acagacgatg aattacgaga cttcttcgat ttgtttttcg gcatatttat
    13921 caaagcagga attgaacgtt ttagcgacat cacggtaaat cccctacaga aattacagga
    13981 aattgatcga ctgagaaaag aaatagctcc agaagaagct gaaatggtgg acaaactcat
    14041 tcggctcctc tttgataccc gttatcaaat ctggagggac aatattccaa gccctgaaat
    14101 ctctctacca gattggcgga aacttttcca aagaaataca actgaaaata aagaaaacta
    14161 gctcaaccac ctgagctagt tttctttatc aaatcttctt aaaaattgag ataatttttc
    14221 taatcctttg attagagttt cttgttcaca ggcaaaacct agacggacat agccgtcacg
    14281 ttcaaaccga ttaccaggaa ctaggagaac accgtgttct tgcaagagtt gcaagcaaaa
    14341 atcttccata ggcatatcca ctgcaatctt gacaaagcta gtggaaacaa ccgctggacg
    14401 gatataggaa acgaggggtt cttcctcaat ccattggtcc aaaattgcta aattttcttc
    14461 taaaatgtgc cgatttcgtt ctagaatttc tttataatgg gccaaagcta attgagccac
    14521 cagatcgtca aagacacctg cacaaatcat agtgtaatcc cgataatctc tcagaatgtc
    14581 cgtcacttga tgattggcgg ccacccaacc aatacgaata ccaggcaggg aataggtctt
    14641 ggataagcta tttacagcaa ttcctttatc atagacttct ataatagatg gcacatccag
    14701 ctcagaaaag gaacgataga cttcatccga taggatgtag gctccaactt cactagcaat
    14761 ttctaccaat tcttccaaat atgtcctatc catgacagca ccagttggat tattggcatt
    14821 attgatgcaa atcatcttag tagtgggacg aatgagctga cgcagttttt ccaagtctgg
    14881 caaccagcca ttttcttcct caatctgcca caaatccact tctgctccca aagactttgg
    14941 aatatcgtag agttgttggt aggtcggata aagggaaatg acatggtctc ctggttcaat
    15001 caaactataa agaactaaca agtttgcacc tgttgccccg ttggtctgga gaatttgttc
    15061 tggcttgacg cctgtataga gttgactaac tgatttttta aatgctggtg aaccttctat
    15121 ccagccatag ttgagcttgg tgccctgcaa ttttttataa aaatcctcag gattggtgcc
    15181 tgacaaggca aataattcct ccaaagtcaa tgaagaaata gacactcctg ctatatcata
    15241 aatcgcgcta ttttcatgga cattcagcca ttcctcaacg ccaaaacgtg gtaatttcat
    15301 aagacttcct acttttattt atcctgctct tattgtacca tattctttca acaattgtat
    15361 aaatatgcga tacatcataa aaggaatatt tttacaattg aaaaagcagg ctttctcaaa
    15421 cgagactgcc tgctctgctt ttttccctta aaacttccct agattcgtca agtctttttt
    15481 gtctggcatg acttccttgt tgtcccagtg ctcgacaatc ttaccgtctt tgaggcggta
    15541 gatgtcaaag tgggcgtagt cctgacctgc aatccagact ttcgaataag ccacgacgta
    15601 gtcgccctgt cccatcacct taaagacaaa gtcgtagttg acagccttat cgaccagata
    15661 atccttataa gctgcaccgc cttgagcgat ttcctggttg tgctgaataa gattagctgc
    15721 cacatagtca tcaaacttgt cgattttccc attttgaagg acatcgacca agaaacgacg
    15781 gacgattttc ttgttgtcct cagtcttatc taggtcagtc agctcaaagt ctgcgtaaat
    15841 cgggtctgtt tggccaatgg tttttggata ggcatcgata acatcccaat gctcaacgat
    15901 acaaccattt tcatccgaac ggaaaatgtc tgccgtcacc cactcagccg caccgtcatt
    15961 gagtttctgg tggacatgga caaagacaaa atttccgtct tcaatggcac gggcaatggt
    16021 gatttcccgc ttgggattgc gtttgaaaaa gtcctcaaag aaagccgcaa agccctcttt
    16081 ctcatcaggc acacccgtac tgtgctgggt gtaactggca cccatataat tttcatgaac
    16141 ttccttgatt tccccgtccc gaatgccacg aaggtaaaga tttttagcgt tatctagttg
    16201 atgtgacatg atgttctcct attttaacaa tccttcttgt tctaaatatt ccctcgccac
    16261 ttgctctgcc gacttgcctt ccacatcgac cgcatagttc atccgcgtca tttcctcggt
    16321 cgagatgttg cctgccaaga cccctagggc ttgctccaac tcagggtatt tctctagggt
    16381 ttctttggac aatagcggag cagcctggta gggcgggaag agccgtttgt cgtcttttag
    16441 gattttcaat ttgtaagtga ctaccttgct gtctgtagaa tatgcttcga taatctgcac
    16501 attaccactc ttaatagctt cataacgaag ggctggctcc atggtcttga cattgagttg
    16561 gagattgtat agatttttta gaccgatatt accatcttcg cgatcgttaa actctaatga
    16621 gaagcctgca acagctgtct gctgcacttt tgccaaatcc gaaatctttt caatcccatt
    16681 tttctgagca taatcttccg ttacagccaa agcataggta ttctggaaag ccattggtgc
    16741 taggtaaacc aagccatcct gctccaaaat cgcctctttg gcataagtgt aaacctcatc
    16801 agaattagta gataaatcca ttgatgaatt tttcaaaaga gtacttgtaa tggtccctgt
    16861 gtactcagga taaatatcaa tagaacctga tttgagtgct tcatacagga agctagtctt
    16921 tccgaaatta ggttttatct ccaccttgat gtctgtctgg tcctcaatca acaacttata
    16981 catattgatg agaatttcag gttctgcacc gagcttccct gcaatgacca actgttttgt
    17041 ggaaaagtta agcatgggaa cataactagc tccaacagta aataaagtca acaatagagc
    17101 caataaaatt gtcttgggtt tcttgtcctg caacagacga atgccataac caaaaagtac
    17161 tgcaaggaca gccgacgata ccgcaccaat caaaatcaag gctgaatcat tgcggtcaat
    17221 tccaagtaga ataaaggagc ctaggcctcc agcccccacc aaggctgcca gagtcgccgt
    17281 tccgataatc ataatggaag ccgtccgaat ccctgacatc aaaataggca tggcaagagc
    17341 taacttaaat tttttcaact tctcccattt attcatacca aaagcggttg ctgcttcttc
    17401 cagagaaggg tcgatttctc caagtcctgt caatgtccct tgcaagatag gaaagatagc
    17461 ataaataaca agtgctacta ctgctggcaa ggttccgatt cccataaaag gaatgaaaag
    17521 accaagcaaa gcaagggatg gaatagtttg gaagatacct gttatttgca gactccactc
    17581 ggtcaagcgt tttttactag atagaatcag tcccaaaggt actgcaattg caatcgcaat
    17641 aatcaaagct agaagggaga tccgaaggtg ttcaaaaaga gcaactcccc actcatcctt
    17701 gcgttctata aaggtttcaa ataggttcat tacatacctc ctcgagctct gataaattcc
    17761 ttgacaaaat cattagcagg atgattcctc aattcatcag gacttcctaa ttgcacaact
    17821 tgtccatctt gcataagaca gatgcgatcc gccaatttaa ccgcctcatc catatcatgg
    17881 gtaacgaaaa ccgttgtcat cttgaattcc tcatgtaatt ccttaatcaa gtcctgcaac
    17941 tgagccttac tgattggatc cagagctgag aaaggttcgt ccatgagtaa cactttcgga
    18001 tttgcaatga tggcccgaag aatgctaacc cgctgttttt ctcccccaga taagtccttg
    18061 ggcaaacgat ccagatagct gtctggctcc aagccaacct tggtcaagag ctctctcgtc
    18121 ctcgtcaagg tttcttcctt acccagacct ttcatttcag gaataagagc gatattttca
    18181 gctaccgtca tatttggaaa aagggctatc tgctgaagaa cataacctgt ctccaaacgt
    18241 aattcacgca ggtcgaaatc cttaagtcgc ttaccttgaa aaaaaacctc tccctcggtc
    18301 tgctcaatta gacggttaat caatttcaac gtagttgtct ttccgctacc actcggtcca
    18361 atcaagacaa agaactcccc ttcttgaata tcaaaggtca aatttttcaa gattggtcca
    18421 ctaaccttac aggtcagaga tacattttga taatcaatca tcatcttctc cttttaaaat
    18481 agccatgaga ctctgactgt agcgctgaat taatcctagc ataagctcct gttcctcctg
    18541 actgagttga gacagcgcct tattttccgc ctcctctaag ggatccaaaa tgcgactagc
    18601 aaattgttgc ccctcctctg tcaatttaat tttcttatgt cgcttatctg ctggattctc
    18661 ctctaaaact accaggcctc catccaaaaa tttctttaca gtagcattga caacttgttt
    18721 gcttgaatag gtcttcttac tgacccagtt ttgcgtcaca cctccgggat tataataaat
    18781 ccacatgaga atggataagc attttgcatt gagcccatgt ccacgagcat agtcttcata
    18841 aaggccgact tgctggtcaa acatacgagc ataatcctta ctccttttct caacttccat
    18901 catccttccc cctcctcagt gagcacaact accttactaa aagcctctgg tctactagtg
    18961 ttcaaaacaa caacaatttt cacaacgact tcctcctgta atcatgttag tcaatatttt
    19021 ttactatttt accaaaaaaa gaaaaatagt caatcatttt gactattttg aaacatcaac
    19081 taccttatta tagattcccg tcttcatcca ctcaaacata ttaaatccct gatattatct
    19141 gcttttaatt tacacaaaac aaatgataat cagataacaa aactagccta gaacattcta
    19201 agctagtttc attttatcct tcaactgttt ctgaggcagt ttcgcttgct acttctgaaa
    19261 cggtttctga tacaggttcg atgatgacat catcaattgt aagctcaata tcttcagaca
    19321 caactacagc atcctcaata tcttcttctg tcaaatccac tgtcggagct ttagcagttt
    19381 ttgccttgat agcttccaaa atgtcagccg ttgacaagtt ttgttccgca attttttcct
    19441 tgatttgctc aaaggtttcc aaggattttt ccttaccaga cttgaccaaa tcttctactg
    19501 tcaattcacc agtttcaagt tgagttttta cttcggtaaa tcgttcagtc gcttgtttgc
    19561 ctaggtcttg tgccttattg acaaaatcag cattgatttc ttcatggttt tccttatagt
    19621 cattagcaaa gtttacaact ttttctttga ctgttttacc agttttactt gctagaaagg
    19681 cagctgcagc agctccgcct gctgccccta acaaaaggct agtccacacg ctactagatt
    19741 tttttaccat ttttgtctcc tttcttacct aagagagatg aaagagttga cagagctgaa
    19801 agagctccgc cagcttttac agtacttgct ccagcagact tggctttgac cgtcaaatca
    19861 cgcgctgaag cattcaaatc tgatacagaa gttgacaagt cagctaccgc tacaaaaaga
    19921 ggatcaaggg tcgaaacctt gccatttaca tcttcgacta aaacattggt tttagccaaa
    19981 agctcgttgg tctgatagag agtcacattg acatcgctgg tcaagacctt aagcgtttgc
    20041 tgggcttcat ctgtcaccgt acccaatttt ttcaacaaca aaacaatata tactgccacc
    20101 gccgcgatgg acaaggcaat aatcaagaca gaaatttcaa taatcatatc ttctccttta
    20161 gggttgatag aatggaatag aagactttct tctccgcaac acaaccatga taataccaac
    20221 gaggattaac accccagata gccattgtga aacacgtatt cccaagaaca tgaggctgtc
    20281 cgtccgcaag ccttctatca aaaggcgacc acaaccatac cagactaggt agaaagctgt
    20341 tatttctccc tgtttaagga attttggccg tctacgtaac acacaaacca agccaaagcc
    20401 tagcaaattc cagagagact caaacaagaa ggttggttga cggtaggcac catcaatata
    20461 catctggtct cggataaagg ctggcaggta gttcaaactt tctactgctt taccataggc
    20521 ctcttggtta aagaaatttc cccaacgacc gattgcctga gcaatcatga ccgatggcgc
    20581 tacaatatcc aaaaaatcca gggtattaat aaagcgatac tgggtaaaga aataaaggac
    20641 gattgcacct gtaatcaaac caccatagat ggcaatacca ccgttccaga tagcgaagat
    20701 agataaaatg ttatccttgt attcgctcca tgaaaaggct acatagtaaa tccgtgcccc
    20761 caggatagaa agcggaaatg cgattagaat aaaatctaaa atatcatcct gacggatttt
    20821 ctttcgtggt ccctcttttg tcgccaaata aacacctaga atcagaccaa gcaagataca
    20881 gattgcatac caacgaattt ctaagggacc taattttatc gcaataggat ccatattaaa
    20941 cctcttctct attgtgctca attaaattag acaagcgttc ttcgaagatt ttcgttgcat
    21001 caaaccccat ctgtttggca cggtagttca tggctgctgc ttcaattaca acagaaatat
    21061 ttcgaccggt ttttacagga atacgtattt gtggaatagc aattcctgct atttcgatac
    21121 tatcgccaga atttcccaaa cggtcaaata ccttacctgt ttcaaaattt tctaagtaga
    21181 cagccaactg cacttctgat gaatctttca cggcactagc accatataga ctcataatat
    21241 cgataatgcc aactcctcga atctctaata agtggcgcag aatttccgca ggttcacccc
    21301 agagagtcac atcgtctttt gcatagacat caacacggtc atcagccacc aatcggtgtc
    21361 ctcgtttaac caactcaaga ccagtctcac tcttaccgat accagaatcg ccctgaataa
    21421 gcacacccat accgtaaatg tccatcaaga caccgtgtac actcgtccgc tgagccaggc
    21481 gactatccaa gtaagaggac aattctcccg atagacgact ggttgctgtc ttgcttcggc
    21541 agatagcaat ttgtttttct tttgcagcct tgtacatctc ctcagggatt tccaagccac
    21601 gtgccactat gacaacaggg gtttctggct ggaacatctg agaaagaacc tgatagcggt
    21661 tatgggcagt catggccatc aaataagacc actccttcat ccctatcaac tggatgcgct
    21721 ctggagcata gtaatcaaaa tagccagtca tttccaatcc tggtcgggta atatctgccg
    21781 tcgtaatttg cttttgcagg agctcttccg tactgtaagc acattcaatc cgcagattat
    21841 cgaccaaatc tttcacataa acggtcattt cttccctcac tttcagtttt attatattat
    21901 accataagct aggggttggg ggaaaaacaa ggggctgatt tggtaaaaag aatgagtttc
    21961 tttctttttc tcacaaaatt agcaaaactt ccacagatta aatgcacttc tcttccttcg
    22021 ctacatattt ttaacaaatc tgttatactt tatatagatc aatacttact aataaggaga
    22081 aaatcatgga accattgttt ctcacacctg ttatgcacga aaaaatttgg gggggcaatc
    22141 gcctaaggac caactaccac tacgacattc ctagtgaaaa gactggggaa tgttgggcaa
    22201 tctctgccca cccaaacggc gtaacaactg tctcaaacgg tcaatataaa ggaagaggac
    22261 tggatgacct ctataagaat gaaaaacatt tatttggcaa tccaacagat gatgtctttc
    22321 cattattaac aaaaatcctt gatgcagacg attggcttag cgtccaagtc catcccgatg
    22381 atagctatgg cttggctcac gaaggcgaac tgggcaagac agaatgttgg tacattttgg
    22441 aagccgaaga cggagctgag atcatttatg gtcacaatgc ccaatccaaa gaagaactcc
    22501 gtcagcaaat cgaagccgga gattgggaca agctgttgac ccatgtaccc gtcaaaaaag
    22561 gtgatttctt ctttgtacca agcggcacca tgcacgccat cggtaaggga atcctgattt
    22621 tagaaactca acaatctagt gatacaacct accgtgtcta tgactttgac cgccgtgacg
    22681 atgcaggcaa cctccgtgaa ctacacattg agaaatcgat tgacgtgctg accatcggac
    22741 ctgttgctaa ctccacacct gcccacctca aagcaggcaa tcttgactcg acacttctgg
    22801 ttgccaatcc cttctttacc gtatataaat ggaatatcca acaagaaatt aaaatggaac
    22861 agacagttcc ttacctgctt gtcagtgtca ttgaagggga aggtgccatc caagtcggtg
    22921 aaaccagcta cccacttcaa aaaggaagcc actttatctt accagccaat gtgacagact
    22981 ggaaatttac aggccaaatg aacatcatcg caagccacac aaactaatac tcttcgaaaa
    23041 tcaaaattat ccgttgtcaa cttgccttga tgaactcaag ttctatcttc gacttcgttt
    23101 cctaggctac ttttgatttt cattgagtat aagcatacaa aaaacttgag cctttaaact
    23161 caagtttttt tgcattattc aaaccaagac ttatttatct tttccgcttc ttttatctta
    23221 cgtggacctg ttccatagcg ttccgcgtcc ctatcttcct tataaggtaa aaaataagct
    23281 gctgcaatat agagagcaat cagaaggagg aaggatttgc ctaagaggag acctgcaata
    23341 aatatcgcgc gtgttaccca ggattcccag cccaatttat gcgctatgcc ggcaagaacc
    23401 ccagatacta tcttcccctg ccttacttta tataattcaa ttgacatctt ttattccttt
    23461 cacgtaaaag cccccttgac ctgtttgtgc cttcaagaaa agtaaatcat tgccagattt
    23521 ttcaaactga tagggactat ttttcttttt ccggttaacc aagacatgac ctgattgcga
    23581 ctcaatctgc ccccgaattc cctggccttt gccagtttgt aaagtcaagg gagcctgagc
    23641 tagttgcaca tacaagtgtt ggggataacc accgcaagtc gtcaagcgaa tatcctgttg
    23701 ttttttatcc cttgaactgc ggatagaaat atcttcaaat ggtaagtaat ccaagcgagc
    23761 atctgcctga ccaagttcca aatccagcac tgacatccat tcctgaggca agttgattgt
    23821 caaacgacag gccaagctac tgtgaaaata aagccccttt ttttcaacat aaagacgagg
    23881 aagcttatct tctggtgcat cttgattgta gcaaatctcc acactaatct gattattttt
    23941 agacttagcc aaagttagac gtccctcaga taattttaaa aagagtgatt caatcccttc
    24001 aaaactataa gattgtttca atttggagga agcaaagacc ttaaaacttg gcaaggtaaa
    24061 attcaatttt actttttctt ccacaaaaag aaagtctggt tcttgattca tttccttaaa
    24121 acaggcacgc gcctcttcct tggtcaagac acctttttca tataactcaa ttaagcgttc
    24181 ttctctcttt tgcgtcatag actcacctca attctactct agtatatcac atttctccac
    24241 aaaaaaatca gcccgaagac tgattctcta ttctttcaaa gtaagttttc ccctacactt
    24301 cccacatcga tattttttta ggtcaatgcg gcgcttgcgc tggtagactt gcccacagga
    24361 ctgacaggta tacagcagac taggcttggc aacttgttcc agtttgggag catagcgcag
    24421 gccgtccacc gcagccagca gttccttgaa gtccttgtcc ccatgccgat agcctttctt
    24481 ctcaaagtag agatggtagt ggcagagctc gtggcggaca atcttccgaa agacttctaa
    24541 accttgctcc tccaagtgct tgggattgaa atccaaatgc ccatcccttg gaaaaaagcg
    24601 accacctgtt gagcgtaatc gcctgttcca gattgctaca tgccgaaatt ccttaccgaa
    24661 gtcttgcaag gagacttctt gcacatactt attcagatcc atttggtttc aaaaggctaa
    24721 gaccgattcg gttacgtttg atgtccactt caaccaccca gacggttacg atttccccaa
    24781 cagcgaggac ttcgctagga tgcggtaagg cttgttggcg tccattttta tcccgattgc
    24841 gtttgaccat gcgtgaaatg tggactagac catcctcgtg gacaccgata tccacaaagg
    24901 ctccaaagtc cacaatattg cgaaccgtac cttgcaattc ctgtccaact accaaatcct
    24961 tgacatccaa gacatcttga cggaggactg gtgcttcaaa gtcatcccgt aaatcacgac
    25021 ctggcttgag caagtctgcg acgatgtcct tcaaggtttc ttgaccgaca ccgatttttt
    25081 ctgccgtatc tgcgatagca acctgcttta atttttcctg cgcagcctca tctaagtggt
    25141 caatagctag aagttctaac agattttcca cagccttgta agactcaggg tgcacgccag
    25201 tattgtccaa aaagttagtc gcatcaggaa ttcgcaagaa accagctgcc tgctcgaagg
    25261 ccttgtcacc cagacgaggc acctttttaa gttgctgacg agaggtcaga gcaccatttt
    25321 cctcgcggta cttgacgatg ttctccgaaa tggtcttgtt aagaccagca acgtgagcca
    25381 aaagggcagg actggccgta ttgacattga caccgacctg gttgaccacc gtatcgacca
    25441 caaaatccag actttcagac agagacttct ggttgacatc gtgctggtac tggccgacgc
    25501 cgattgactt gggatcaatt ttgaccagct cagccagagg atcttgcaga cggcgggcga
    25561 tggaaatggc agagcgtttt tccacaggta ggtctggaaa ctcatatctg gccagttcag
    25621 aagctgagta gacagaggct ccgctttcat tgacgatgac gtaagaaaca tcagggaagt
    25681 ccttgagtaa atcagctacg aaggcctcag actcacggct ggccgttcca tttccgatag
    25741 caatgatttc cacttggtat tgtccaatca ggtctgccaa gtcttttttt gcctgagcaa
    25801 tttgtcgctg accagccggc tccacaggat agatgacttg tgtagtcaag agttttccag
    25861 tctggtctac cactgctagc ttggcccctg tccgaaaggc tgggtcaaaa ccaagaacaa
    25921 ccttgccttt tagaggagat acgagaagta gattgcggag gtttttagag aagagctgga
    25981 tagctccttc ttcggcttct tctgtcaatt ctgttcgaat acggcgctcc atggcaggca
    26041 gaattttctt cttaatagcc tgctgaatga catccttgat gtagctatta gactgtggaa
    26101 aacgcaactc aaagaagcgg atcattttat ccacattgtg ttcaaaagaa accttgagaa
    26161 cacctaattt ctcaccacgg ttaatggcca gggtcttata gccctgcatc ttcgctactt
    26221 tctcagaaaa gtcatagtaa atctgaaaga cttccttctc atctgcttct tgatctttta
    26281 gctctgaagt caggtttgaa ttggtttgta cttcatgata aacccatgcc cgaagcttat
    26341 tgtcttctga gatagcttcc accaaaatat ccacagcacc agccaaacaa gactgagcag
    26401 tgtcaaagcc ctcgcagata aagtccgcag cctgttcttc caagtcagcc acattttgca
    26461 aaatcaatcg agccagcggg aacagtccag cctcacgcgc caccgttgcc ttggtccgac
    26521 gtttttcctt ataaggcagg tagagttctt ccacatctgc caacttttcc gcttcctcga
    26581 tagcctgtcg aagctggtct gtcaattttc cctgttcttc aatcttggca aggacagtag
    26641 ccttacggtc agcgagagca gtcaaggcct tatcacgatc aatgatagac ttgatgacca
    26701 cctcgtccaa gttccccgtc acatccttcc gataacgagc gataaaggga atggtgttgc
    26761 cttcagccgt caaagagaga accgtatcaa tctgttttaa actaacgccc aattcctgag
    26821 caattttcat gtatttttct tccatagcta ctattatagc atgaaagcgg tacaaatgac
    26881 tcaatagaaa aagcacgtag ataaactaca tgctctcttt attttttctc ttctgatcac
    26941 gttttttcac aaataaaatg attaggagag ataggaggaa tcctaataca ccacaaacca
    27001 cttgaacagc aaggtaaatc tcatcaactt tcttttgact agattgggct ttttcgtaat
    27061 ccttcttatc atacggaact cgtttcccgc gaaccaaaat ccgttctgta tttattccat
    27121 aaggtgtaca agtgatcaat gtcgcataat cttctccctc aataccggac agtgaactta
    27181 cctgattcgg cttcacaact tgaatccaat caacttcgta agcaagaatc tctcccaaaa
    27241 catggaaata gaattgatct cctttttcta attgcggaag attggtaaac atttctgcat
    27301 tcggtaaccc tctatgggct gatacaaccg tatgactacc atctccccca acgggcaggg
    27361 cactaccaaa taggtgacca gctccttttt gtaagacact gtcgcttgta tagtgataga
    27421 tgggtaattc aatatcgatt tttggaattt ctacataccc catcatccca tcctgagagg
    27481 catttaacac tgtctcatac ttaggatcat gaatcccctc acgtaaggaa aaggcatcag
    27541 gtacaggctg aatccctaac tcattattat aggattgagc aacttgccac attttttcct
    27601 tttcttgttc tggctgagta tctacccact ctctatgaga actgattaat tgttgatcac
    27661 ggtaccgatt ccaactgcta ctgatatacg gatacaataa aatcagtatc cccactaaaa
    27721 taaggaaaat atatctgata cctgctcttt gtttcacgtc cttctcctaa aaaatgtaga
    27781 gaaggcaacg ccttccctcc atacttgttt attaattctg ttacttatgc ttttttattt
    27841 ttagtaaagt acaaacctaa gccaactgta agcactaaga caccgataaa tgtgaatagg
    27901 tatgttccca ttccacctgt tgatggcaat tctcctaatt tggtatttgg tacgtcagca
    27961 atagatactg tacccgcacc agcagttgga ttagttgttg tcacagttgt attttgtgcc
    28021 gtagtagttg ttgctttcaa ataggcagct tgtacatcgg taccttctgg tttattatcc
    28081 aatttataaa agacattgtg tttcaaccaa ccaacttgtg tgctatcctt tgcttcaccg
    28141 acaactgagg tgtattcact acgagttgag cttgcacttg aagttgttgt tgctgaggtc
    28201 cagcttgctt cagcagtata aacattggtg tttaatgagt agcctgttgg tgcagtaatc
    28261 tctttcaaat agtaggttcc cttaccaacc tttgttgaga tagcataacc atccgcattt
    28321 gtagttacaa tatctactaa ttgcttagtt tccgcatcag agtaaattcc gaaaactgca
    28381 ccagcaagag gtgctttcgt gatagaatct acttttttaa aggcgatttg gtaagtgtag
    28441 actgtctcac tatcttcttt atttacgata gtattgtcat tttgaggatt tggcttatta
    28501 tttggatctt cgtacgtatt accactgtta gggttacgcg catagaagaa ctcagccttg
    28561 ttggcatttc ctgttccacc aacaacagcc ttgtcattga tgataccttt ataggtcact
    28621 acaggtgcaa tttcattcaa attatcatag acaaatgcca gattaaagcc attgccaact
    28681 ttaactgcct tagcaatcaa aatatcccct tgagttttaa attgaccagt agtgtcagca
    28741 atcaatgttt gaccattcca agaaattgtc aagcttgcat aatcaaatgt caaaccttca
    28801 gacattgtat ctgaaataaa aattgtcttg tttttagcct gagttggata gcttggaagc
    28861 gtcacattca aggaataagt caatacttga cctagtgaag cagaattttt atcagcatct
    28921 gcagtcactc ctgaaactgt cttgtcaata cttggaagag ttgatttggc tacagaagtt
    28981 tgaccataaa gatatttagc ttttgaatca acacttccac ctgtcaattt accttcaccg
    29041 ttgtaagacg ctgcaagcaa gattgggttg aaggaacgac catctgtagc tcctgtcaaa
    29101 atagcaatat aaacccctgc acctgtcgct ctataggttg cagtcccatt tgtaacttgg
    29161 agaacttttg atgctttggc ttgaattgta ttgtctaaaa taccattagc aatgtttgta
    29221 atttcttctg cagttggtcc agtttctgtt agtttaactc cacttgcata atcgaaacga
    29281 ataaacgagt cattcgttgc attatataca ccttcaccga ttttataaag ggtaactgta
    29341 ggatttccct caatgttggt aatttttaat tctgcttgat ccgtatttga tgcagcaaat
    29401 actggacgtg cattcattcc taaaacggtt aaaagaactg ttaaaagagt gaatattctt
    29461 ttcattttct tctcctttga gctttaaaaa attaagcaat ctgctttctc ttctcataaa
    29521 aaagatagct tgctaatgta ataaccatca cacctagtag gatatagacc gcgggaccat
    29581 acccacctgt agatggtaat tgataaagtt tatggttcac aacgcgatat tgttcaccgc
    29641 ttttacttac tgtataacct agttgatctc tgatggtaat cgtcccatca acggcaacgt
    29701 ctaccgtcca aggatcctct gggagagtat agccatcttt agccttggtt tcatatagat
    29761 agtacgtttt tgctcgacgt aaatctgtaa aggttatatt tcccctacta tctgtctcac
    29821 cgcttgcaaa gactgtcttc ttgtcagata accgtaattc aaaacgaaca ttttctagca
    29881 ggagattagt ctccttacta tcactttttt gtagggtaaa ttgacgtgta tctacttgca
    29941 ccacaggatg gtcgtattct ttttcatagg gttgttcatt tcgaatatag gtcaatgatg
    30001 ccaccttgtt agatcggaac ccagcctgcc cagaactggt actgtttccc gtataatcag
    30061 ttccagaatc cccgtgtgca ttataaccag acgtcttctt ttcctcataa gcacgatctg
    30121 tcacttttac attataagaa attgtataag tataggtatc atcaatttta aaattatcta
    30181 caaaattcaa atcaaccttc cctgtagaat cactagcatt aacagccgta tgtgttactg
    30241 attgtaaaat acctactccc tggctcgtta ccgctccatt ctgatagagc gttgtaacct
    30301 ggcccgtctg acgattcgtt tgtaccactt tcacatccgg ctgttccgcg taataagata
    30361 catattgact taactggtca ttgatggaaa caagagacaa ttttgtatca tcaactatct
    30421 tttggagagt ccgttcaagt gcttgtgaat cggcaacatg cgaataaaat cctcccgtct
    30481 gactagacat gtgattcaat acatatggat tagcagacaa gttgtcacta tttccaaact
    30541 cactagaaat ccccaaggta tagataggta acttaggata cttacctatc caactgttga
    30601 aatagttaag cgtccaatcc ctcgtataat aaggattatt tccgctaatg gtaccatttc
    30661 cgtatcgaac accgttcaca aatgcaaagg tcggcacccc atctgaaata aaaatcatta
    30721 ccttcttccg actactttgg ttctgatcta gaacaacacc agctgttcgt aaaccagccg
    30781 tgtaattggt tccgttatta taaggtggct gaatattact cagtgcaacc gaggtagaac
    30841 gtccccagtt agcaataacc gttgaatcct gatctccgct tggataacct ggataacctt
    30901 gaaaaccaac tattgctact tgattttccg gatgaaaacc aaccaactta ctaagcagcc
    30961 cttgccgccc tgcagttccg tttaaggctt tataaacggc tagatagcga gcctgtgtgt
    31021 ctacactatt catcggatca gtcatactgg ttgacctatc taccacaatt agagtatcaa
    31081 agggctcctt tgtccccgtc atatctagat acatccgata gagttcatgc gtatctgtac
    31141 caatacggtc atcaacacta gtatccggat tattcccacc atcacctaaa tagtcaatcc
    31201 gtttagactg cgtgatgtgg gcctgatcta ccgacgaatt cgatacagtc gcattcgtta
    31261 cacttctaaa tccactaaaa gatctgtacc ctaacatcct cgtgcgaagc cccaaaatca
    31321 taccagtacc ttcgagcatt cggttctgga gataaatttc tagaacgtta ctctcttctt
    31381 caccactttc ttgatgaaat gatgcaacag gaaattcgcc attgaaatac attaagctat
    31441 cggttttaga ctgatgagcc tgacggtaat tcattttgac tatatcaaac ttctcgctct
    31501 caattcccaa taaattaaaa ctaggtttcc aagtggataa gacatcaaat gtcttttctt
    31561 ctcgaaatga atctattgga cttaagatag taaagatacc tgaagcatct gaacgaaaaa
    31621 ttttcacttt tagccgtgca tttaaaggaa cattactagc tgtttgttct tcttcctctt
    31681 cgagaacgta cccgtctaac tgcatctgaa catattgtcc caatgtcact gttgtctttt
    31741 ctgctaatgg cttaaccatt accacatgaa tatcttttcc aagtattcct tcataggtca
    31801 atcctatcaa ggtacctgtc gtatcaaagt gaaattcagc atcatgaaaa accaaggagc
    31861 catctgtatt ggtcgaaacc aaccgaagat tttctttggt cactaaaccg ccacgcaatc
    31921 ccagagaagt cttgaaatta actgtcaacc ttggtttata accttccttg acaatctgac
    31981 tatctgaaat ttctatctgc tgaacatcag caggctgtaa attctgattc acgagatatt
    32041 gactaagttg atttacatac tctctttcag aatcccactt acccgagttt ttcatccgag
    32101 gtagagttac gctaaagttt tcaacaccat taccaaagta tgcgtttaaa tcagcagtca
    32161 catactcatc ttcatagtgc aggatttttt gaacagattc ttcagaatta tccgccaaat
    32221 cctgagaaga actaggtgca tctgaccgat tcgtttcggt attttcctct gcttgtttca
    32281 tttcaacaat ttcttctgat gattgactca tttctgtcgt tgtttcactt ttagaaacag
    32341 tttcttccgc tgaagaaata actgaactac ttgttgactc actcatattc tctaacgcat
    32401 aaccaattgt tggactgcag ttactcaaag taagtaacaa aatcgcaaag atggaaagcc
    32461 atctttttcc aaacataaat ttcatattcc tcctttccat aagaatcgaa atttgctata
    32521 ttaatcaaat gatt
//
LOCUS       DAZQCJ010000013        32200 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000013 DAZQCJ010000000
VERSION     DAZQCJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32200
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.013"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            113..457
                     /locus_tag="ACHU66_000552"
     CDS             113..457
                     /locus_tag="ACHU66_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SSU0592/SSU0593 family protein"
                     /protein_id="HFR3388861.1"
                     /translation="MKRIVTKRSYKVGLCAMALALLMSTAGGVTAGAQDYHVESSGEL
                     KPMSEGVFMKLESSSNFIENEGIVVKSISNQNTNPNNLNESWYWKFWKKVKLFFKRFR
                     EGFGIQRLFGFK"
     gene            567..911
                     /locus_tag="ACHU66_000553"
     CDS             567..911
                     /locus_tag="ACHU66_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388862.1"
                     /translation="MKQANSKLAALTLITTLAVTSVIGTTVHAEEKVTADQTVAGKHK
                     PITDEQLQKAIGDRNPNNIKIQAKFENTNFKNQGITFIGKAEIPSKSSENSSWFWNLW
                     TRFTTFVKGIFS"
     gene            1383..1508
                     /gene="dltX"
                     /locus_tag="ACHU66_000554"
     CDS             1383..1508
                     /gene="dltX"
                     /locus_tag="ACHU66_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFR3388863.1"
                     /translation="MQHPVVRFICRTLFYFFILLLLLYLYSYLDIGQGGFIYNEF"
     gene            1525..3060
                     /gene="dltA"
                     /locus_tag="ACHU66_000555"
     CDS             1525..3060
                     /gene="dltA"
                     /locus_tag="ACHU66_000555"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFR3388864.1"
                     /translation="MISSMLERVEHFATENPDYPVYHYLGQLYSYGDLKADSDSLAAH
                     IDSLDLPANSPVLVFGGQEYQMLAIFVALTKAGHPYIPVDSHSALDRIEAILEIAEPS
                     LVFAVADFPLETSLPVLGLSEVEAIFEKKSTYQLMHPVSGDDTYYIIFTSGTTGKPKG
                     VQISHDNLLSFTNWMIRDKEFALPERPQMLAQPSYSFDLSVMYWAPTLALGGTLFALP
                     KELTLDFKTLFAAIQTLPFKIWTSTPSFVDMALLSREFDGEHLPDLTHFYFDGEELTV
                     KTAQKLRERFPKAVIVNAYGPTEATVALSAVAITDEMLVNYKRLPIGYAKPDSPTFIL
                     DEDGQVVPNGQQGEIVVSGPAVSKGYLNNPERTEAAFFQFDGLQAYHTGDLGVMTDEG
                     LLLYGGRMDFQIKYNGYRIELEEVAQHLNKSTYIEAAVAVPRYNADHKVQNLLAYVVP
                     KAGIMEQFDRKLELTKVIKQELKERMMDYMMPSKFIYRDSLPLTANGKIDIKGLIAEV
                     NAQ"
     gene            3057..4298
                     /gene="dltB"
                     /locus_tag="ACHU66_000556"
     CDS             3057..4298
                     /gene="dltB"
                     /locus_tag="ACHU66_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFR3388865.1"
                     /translation="MMEFFKQLPQLEPYGNPLYFFYLVLALVPIFIGLFFKKRFPLYE
                     TGVSLAFIVLMFTGTKTMQLLSLLAYIIWQTMLIFFYKHYRQRANQSWVFYLIVCLAV
                     FPLTWVKLAPTFSQHGAIFGFLGISYLTFRSVGMVIEMRDGLLTEFSLGSFLRFLLFM
                     PTISSGPIDRYRRFTEDYKQIPERTELLNMLDQTMHYIMMGFLYKFILAYFIGHTLLE
                     PLKAVALDQGGWFNLPTIGVMYLYGFELFFDFAGYSMFAIGISNLMGIRCPINFDQPF
                     KSRDLKEFWNRWHISLSFWFRDFVFMRLVKTLLKHKVFKNRNTVSNVAYLLNMLLMGL
                     WHGVTWYYIAYGLFHALGLIINDAWIRKKKSINLARKKAGQEPLPDNRWTSFAGMFVT
                     FHTVMFSFLIFSGFLDKLWFK"
     gene            4341..4580
                     /gene="dltC"
                     /locus_tag="ACHU66_000557"
     CDS             4341..4580
                     /gene="dltC"
                     /locus_tag="ACHU66_000557"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFR3388866.1"
                     /translation="MDVKETILNIIEELFMEDVSEMMDEDLFDAGVLDSMGTVELIVE
                     LESRFNITVPVSEFGREDWNTANKIISGVVELMHA"
     gene            4573..5838
                     /gene="dltD"
                     /locus_tag="ACHU66_000558"
     CDS             4573..5838
                     /gene="dltD"
                     /locus_tag="ACHU66_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFR3388867.1"
                     /translation="MRKQLWQIFGPVICATFLVILTLLFFPSSSRSNFKEEKQDAVAL
                     SYISFKSKYKKNRALGDKNHRFVPFFGSSEWSRFDSMHPSVLADAYQREYTPYLLGQR
                     GAASLTQYFGMQQIGAHLKNSQAIYVVSPQWFSPKGASSKAFEQYFSSDQATHFLQTS
                     AGDDYDRYAAKRFLELKSDCSLTGMFQKVASGKELSKWDREQLTWQAYLLEKQDALFS
                     RFADSGSNLGLVETEAMKLPETFSYDRLSKVANEDGSRSTQSNDFQIDDRFYRNRLQS
                     KIKKLKQSQTKISYLQSPEYNDFQLVLNEFAKHQTDVLFVIPPVNERWMNYTGLNKDM
                     YRATVAKIKYQLTSQGFNHIADLSEQGGIDYFMQDTIHIGWQGWLELDKYIHPFLTEQ
                     QAPVHYQLKKEFLTSEWATYKGDLNLFLK"
     gene            complement(5927..7021)
                     /locus_tag="ACHU66_000559"
                     /pseudo
     CDS             complement(5927..7021)
                     /locus_tag="ACHU66_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
     gene            complement(7095..8051)
                     /locus_tag="ACHU66_000560"
     CDS             complement(7095..8051)
                     /locus_tag="ACHU66_000560"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFR3388868.1"
                     /translation="MGLLQDGKWVDQWYDTKSTGGKFIRSVTQFRNWITPDGQAGPTG
                     EGGFKAESGRYHLYISLACPWASRTLIMRKLKGLEDHISLSVVHPLMLENGWTFEDGP
                     GVIKDTLFNSDYLYQVYLKADSHYSGRVTVPVLWDKETNTIVSNESAEIMRMFNSAFN
                     DITGNYDDYYPANLQAEIDAMNDFIYPNINNGVYKAGFSTSQAVYEKEVKNLFAALDK
                     LEEHLADKDYLVGNQLTEADIRLFTTLVRFDPVYFGHFKCNIKALVDYPNLWDYTKRI
                     YNHSGIAETVDFDHIKQHYYGSHKTINPTGIVPVGPDLDWTL"
     gene            8190..8906
                     /locus_tag="ACHU66_000561"
     CDS             8190..8906
                     /locus_tag="ACHU66_000561"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFR3388869.1"
                     /translation="MRLDKCLEKAKVGSRKQVKKLFKAQQIKINGQAAQSLSQIVDPE
                     LQTIQVSGKKVALEGSAYYLLHKPAGVVSAVTDQEHQTVIDLISPQDSREGLYPVGRL
                     DRDTEGLVLITNNGPLGYRMLHPSHHVDKFYYVEVNGCLAEDASKFFASGVTFLDGTR
                     CQPAVLTVLEASLDHSRATIKLAEGKFHQVKKMFLAYGVKVTYLKRISFGGFELGDLE
                     RGAYRQLTPNEMEHLFTYFD"
     gene            8920..9399
                     /locus_tag="ACHU66_000562"
     CDS             8920..9399
                     /locus_tag="ACHU66_000562"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFR3388870.1"
                     /translation="MSIYDFTVQKQDGTDQSIAEYQGQVLLIVNTAPGCGLAPQYKEL
                     QELYDSYRGQGFVVLDFPCNQFLNQAPGSAEDINQTCSLNYGTTFPRFAKIAVNGSEA
                     SPLYRYLKKEKSTLLGGRIEWNFTKFLVDRQGRVVKRYLPTTSPLKLKEDIEFYLEK"
     gene            complement(9418..10419)
                     /locus_tag="ACHU66_000563"
     CDS             complement(9418..10419)
                     /locus_tag="ACHU66_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFR3388871.1"
                     /translation="MIRHHNYLKIFTFLLILLFVIFLLSLSIGYSNSSFSDLIDIMSG
                     RASSSTLLIIGRIRLPRIIASIIGGASLALAGLLLQTLTRNPLADSGILGINAGAGLV
                     VALFVGLSSKISPVLLSLMPLFAMMGGGLTIFLVYWIARKKLYGIHPARLIITGVGIS
                     SMLSGIMVSIISQLDDFKMEYIVQWLSGRVNGGDWKTIAIYTPLLLLVWMATFSRSRS
                     LNIMNLNDQTAMALGLHLQRERRITLILATALASLSVILVGNITFVGLIAGHIVRKWL
                     GNDHRVIIPATMIIGSFILLLADTIGRVLLVGTGIPTGIIVSLIGAPYFLWLLKQEG"
     gene            complement(10416..11441)
                     /locus_tag="ACHU66_000564"
     CDS             complement(10416..11441)
                     /locus_tag="ACHU66_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFR3388872.1"
                     /translation="MNKLVSSRYPKTKPKNIWLVFFIICLSFIAGVYLSLRFGAISYS
                     HQQLIETLKHPLTDSSAQDVIIDLRLPRMVAAILVGAAMAQAGAMMQGITRNPIADPG
                     LLGINAGAGLALIVAYALFGGLHYSQILLICLLGSCLAAGLVFGLAYQVQKGYNQLRL
                     ILSGAMVASLFSAIGQAITIYFDLSTAVIGWQAGGLVQVNWKMLAIIAPLIIIGLILA
                     QLFSHQLTILSLNETVAKNLGQRTLLMTLVLLGIVLLLSASAVALVGSLSFIGLIIPH
                     FIRMFTGKNYKLLLPLTAFAGASFLIWVDLVCRSINPPVETPISAVISIIGLPCFLWL
                     IRKEKHF"
     gene            complement(11455..12384)
                     /locus_tag="ACHU66_000565"
     CDS             complement(11455..12384)
                     /locus_tag="ACHU66_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR3388873.1"
                     /translation="MKKFLAIFSLFVGLIFLTACSTSSSSTDVELSSMPKIEGITYHG
                     DIPKNPKKVVNFAYSYTGYLLQLGIDVSSYSLDLEKNSPVFGDKLKDVPQLTTADTEA
                     IAAQKPDVILVFAGDDNLETLKEIAPVIEITYGKSDYLKMLTDIGQIFGKEKEAQAWL
                     DQWDKKVATAKEELKGLIDTSSTFTVMDFFDKNIFLYGNNFGRGGELIYRALGFAAPA
                     KVQEDIINKEGWFGVSQEALPEYVGDYVLVNVNDKTKEAAASLKESDIWKNLPAVKNG
                     HVIEIDYNLFYFSDPMSLDLQIDAFVSEIQKLQ"
     gene            complement(12381..13181)
                     /locus_tag="ACHU66_000566"
     CDS             complement(12381..13181)
                     /locus_tag="ACHU66_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3388874.1"
                     /translation="MSSIQAENIQVSYDNRIIIDELSTSIPKGNITTIIGANGCGKST
                     LLKALTRIIPVQEGAIYLDGQAISQLPTKEVAKKLALLPQVLEATEGISVYELVSYGR
                     FPHQNGLGHLTDQDREKINWALEVTQTAPYARLPVDDLSGGQRQRVWIAMALAQDTDT
                     IFLDEPTTYLDLNHQLEVLELLKELNQSRQKTIVMVLHDVNLSARFSDYMIAMKEGDI
                     RYHGSVSNMMTTEILRDIFSIEPQLMQVAGQDYPILLTYDLKNNKEKK"
     gene            complement(13334..14779)
                     /locus_tag="ACHU66_000567"
     CDS             complement(13334..14779)
                     /locus_tag="ACHU66_000567"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013990079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFR3388875.1"
                     /translation="MITIERVLEILKADANFRHIKQNDQTDSTWTDVQFDALSYDSRT
                     VSPTTLFFAKGLAFKKEFLEKAIEAGLAFYVSEIDYEVDIPAIIVHDIKQAMSLIAME
                     FHGHPQKQLKLLAFTGTKGKTTAAYFAFNILKQSHKPAMLSTMNTTLDGKTFFKSTLT
                     TPESLDLFAMMAEAVKNGMSHLIMEVSSQAYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPTFEDYFYHKRLLMDNSRAVIVNAGMDHFEVVKEQVSSKDHDFYGPTSENQISQSAG
                     FDFTATGKLAGHYDIQLIGGFNQENAIAAGLACLRLGASLEDIHTGIAQTNVPGRMEV
                     LTQQNGAKVFVDYAHNGDSVKKLIDVVLEHQTGKVFLILGAPGNKGESRRKDFGLLLN
                     DYPQIEVILTADDPNREDPAAIAEQIRAHMTRPSNFILDREEAIRTAMSQTSSTKDAV
                     IIAGKGADAYQIVNGEKAAYDGDLEVAKQYL"
     gene            14925..15671
                     /locus_tag="ACHU66_000568"
     CDS             14925..15671
                     /locus_tag="ACHU66_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFR3388876.1"
                     /translation="MFYSYLRTLLTFLLWAINGNIHYHDREKILPQEENYILIAPHKT
                     FWDPVFLGYAAAPKQFIFMAKKELFKDRGFGWWISKCGAFPIDRENPGMAAIKYPVNM
                     LKKSDRSLVMFPSGSRHSSELKGGVAVIAKSAKVKLMPATYVGPMTIKGLLAGERIDV
                     AFGNPIDVSDIKRMDDTGTAEVTSRIEAEFKRLDDHAASFQTKKKPNILTYIYRIPVL
                     LLVALILGLTYAFSYIASFFWQPSTQLDKK"
     gene            15739..16401
                     /locus_tag="ACHU66_000569"
     CDS             15739..16401
                     /locus_tag="ACHU66_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR3388877.1"
                     /translation="MDTIKTYIELLKEYKWQIALPAVAGLLMATFLIFSQPAKSDQTG
                     LTDFPQTEQTSSGQEQTEEISTEVSEEPSQLVVDVKGAVDKPGLYTLEAGARVNDAVE
                     AAGGLTSQADPKSINLAQKLSDEAVVYVASKDENISVVTSTTASSAMSPEEKSTSLVN
                     LNTATEADLQTISGIGAKRATDIIAYREANGGFKSVDDLNNVSGIGDKTMESIRPYVT
                     VE"
     gene            16385..18622
                     /locus_tag="ACHU66_000570"
     CDS             16385..18622
                     /locus_tag="ACHU66_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR3388878.1"
                     /translation="MSRLSKLPCQPVHLAVLAVAAYFAVHSFSLLTMGLFSLLLAVFG
                     VRQGKTVFLKTLPLLALCGLFFGYQKVQWERADQSATEQVTTVQVIPDTIDINGDSLS
                     FRGRADGQTYQVFYKLTSQEEQTYFQKLTGLVQLEVEAEISLPAGQRNFKGFDYQAYL
                     KTQGIYRTVKITAIKKIVPVQSWNVFDWLSNWRRQALVYVKTNFPAPMSHYMTGLLFG
                     DLDSDFDQMSDLYSSLGIIHLFALSGMQVGFVIDKFRWILLRLGLTKETVDKLQIPFS
                     LVYASLTGFSVSVVRSLVQKILGNLGLRKLDNFAVTVFVCLLILPRFLLTAGGVLTFT
                     YAFLLTVFDFEDLGQVKKAAVESLSISLGILPVLMTYFYAFQPLSILLTFAFSFVFDV
                     LLLPGLSVIFLLSPIVKITWVNGFFVFMEKIIVWVADLGLRPWILGKPSELVLLLLLV
                     SLFLLYDFHRRKKWLLGLSLVLALLFFITKHPLENEVTVVDIGQGDSIFLRDMRGRTV
                     LIDVGGRVDFAAKEAWQERPRQANAERTLIPYLHSRGVDRIDSLVLTHTDTDHVGDVL
                     EVAKQVQIGRIYVSPGSLTVPDFVATLKEINVPVHVVEVGERLPIFDSYLEVLYPDGT
                     GDGGNNDSIVLYGRLLKTNFLFTGDLEQGELDLIESYPNLPVDVLKAGHHGSKGSSYP
                     EFLDHIEAKIALVSAGENNRYKHPHQETLERFDSRNIQVYRTDQQGAIRFRGWREWRI
                     ETVRR"
     gene            18639..18764
                     /locus_tag="ACHU66_000571"
                     /pseudo
     CDS             18639..18764
                     /locus_tag="ACHU66_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            18946..19602
                     /locus_tag="ACHU66_000572"
     CDS             18946..19602
                     /locus_tag="ACHU66_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFR3388879.1"
                     /translation="MSVKDFMTRKVVYISPDTTIAHAADIMREQDLHRLPVIENDKLV
                     GLVTEGTIAEASPSKATSLSIYEMNYLLNKTKVKDVMIKNVITVSGYASLEDAAYLMY
                     KNKVGILPVVDNGQLYGVITDRDIFAAFLHVSGYGEEGVRARFLVENKAGELEKIIRL
                     VADKDYNIVSTVQLATKSGKVVIEVQIDGKVDVEEVRTLFEGAGIQVDSLVPTVAKNI
                     "
     gene            19696..20523
                     /locus_tag="ACHU66_000573"
     CDS             19696..20523
                     /locus_tag="ACHU66_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388880.1"
                     /translation="MGHKYKKYKKYGKNNSGLVALLVLLAIGFVMSFWYILLPTGLLI
                     GYFYKREAIQRFLNRDSIKRSQVLALSIRSGYEKHQELFVEKGEDKVVLQLRNRLLGQ
                     LFELDQLYQKVGKYLNGYESKEVVDTLQLKYHLKLPEAKTEKNTDTQVSTGGNAVMDE
                     QGMIADLAPEILETYCNIQRDNLVILEKLEKATDKREELTAIHEANMNRFNDILQGYL
                     KIKKSPKDYFNAEERLVQAKSAMEMFDKDLDDTIKQFNEAGMKDFEVSLRMMKREEE"
     gene            20529..21800
                     /locus_tag="ACHU66_000574"
     CDS             20529..21800
                     /locus_tag="ACHU66_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFR3388881.1"
                     /translation="MSGFNFDIDQIANNSLSTKDKTTELIQATPTGDNSKVSFLAQLT
                     PEQQTGIRAKAPQLVDNFLANQNALLDFGKEAVEEVNATVNHILSEQKKIEIPQVDEL
                     LANTNRELNGFVAKYKDISTTAELEKKPGFFQRLFKQTKNDLQEFYFDSQTIEKKMDS
                     MAASVVKQEEVLARNIVSAELLIENNTKSIENLVGVIAFIEATGQEAAERAKLAQENL
                     ATLVPTTVDYQVASEKLARVTEVANILEQQHSEYMSRLYVAWTTTPQMRNLVKVSSDM
                     KQRLGMLRRNTIPTMKLSIAQLGILQQSIKSSQTADAIVNANNAALQMLADTSKEAIP
                     MMERTAQNPTLSVASVTKLAESLVAQNNGIIAAIDEGRQKRRELEAAIVRSAETINDS
                     VKLRDQKIIAALLEQGKEAQSEAEQPLETNQ"
     gene            complement(21837..22703)
                     /locus_tag="ACHU66_000575"
     CDS             complement(21837..22703)
                     /locus_tag="ACHU66_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFR3388882.1"
                     /translation="MVRLKNILLILLGAGLFAFGLNYLIMPNRLFEGGATGLTLIIYY
                     LFHIQPWIMNIVINIPLFILGWKILGKKTLYLSILGTFSVTFWLAIFEKIHFSINLQQ
                     DLILVSILGGILMGLGLGTIFRAGGTTGGSDIIARIGHKFLPYSIGQIILAVDILILT
                     LIVIVFKDLRTVLYTLMMVAIASKVIDFVTEGGYGSKGVMIVSQKSDQLAQAIDCEIE
                     RGVTFIKAQGFYSKADVNMIYSVIYKSQLQEMKELIHRIDPHAFITITDAHEVLGEGF
                     TLDRDKKPIERH"
     gene            22878..23516
                     /gene="tmk"
                     /locus_tag="ACHU66_000576"
     CDS             22878..23516
                     /gene="tmk"
                     /locus_tag="ACHU66_000576"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR3388883.1"
                     /translation="MRNGFFITFEGPDGAGKTTVLQQLLPALQELGPEVVTTREPGGV
                     AIAEEIRNIILNPANTEMDDKTELLLFIAARRQHLKEKILPPLAQGKLLLIDRFIDSS
                     IAYQGFGRGLDIGDIHWLNQFATDGLKPDLTLYFDIDTEEGLARIARNADRDVDRLDM
                     EKADMHRRVRQGYLNILEQEPERFVKIDASQPLEAVVADALAVIKKRFAERT"
     gene            23513..24397
                     /locus_tag="ACHU66_000577"
     CDS             23513..24397
                     /locus_tag="ACHU66_000577"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR3388884.1"
                     /translation="MKIEELRNLQPNVFQRFVTILEQRRLVHAYLFSGDFASFDMAIF
                     LSQSLFCQEKEGVLPCQKCRSCRLIEANEFSDLTVIAPQGNVIKTDTIRELVKNFSQS
                     GFESNKQVFIIRDAEKMHANAANSLLKVIEEPQSAIHILLLTNQEEAVLPTIKSRTQI
                     IGFPKNIPAMERMLEEEGLLKTQANLLAQLVSSQEEAKKLAENKNFLDLMGQTKKFTD
                     LLLVEPTRAYLQVGILVSLAVEKAEQGRLFDLLSLILSEKMMESANVREKMDAVLKAK
                     KMWQANVSLQNSLEYITL"
     gene            24417..24734
                     /gene="yabA"
                     /locus_tag="ACHU66_000578"
     CDS             24417..24734
                     /gene="yabA"
                     /locus_tag="ACHU66_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFR3388885.1"
                     /translation="MDKKEIFDALDDFSQNLLTTLAEVDAIKKHLQGVIDENTTLRLE
                     NSKLRERLEKEDKTGHKSSNFGKENLENIYEDGFHICTFSYGQRRDNDEPCMFCVELL
                     NRD"
     gene            24736..25599
                     /gene="rsmI"
                     /locus_tag="ACHU66_000579"
     CDS             24736..25599
                     /gene="rsmI"
                     /locus_tag="ACHU66_000579"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR3388886.1"
                     /translation="MKVQKSFKGQTSFGTLYLVPTPIGNLQDMTFRAVQILKEVDIIA
                     AEDTRNTGLLLKHFEVETKQISFHEHNAHEKIPVLIDWLKSGQSIAQVSDAGLPSISD
                     PGHDLVGAAIAEDIPVVALPGASAGITALIASGLAPQPHIFYGFLPRKSGQQKEFFQE
                     KKTYPETQIFYESPYRVADTLENMLTVYGDRQVTVVRELTKLYEEYQRGSISELLEYL
                     EENPLKGECLIIVEGAGEEVYPSADEVDLKAEVEREIASGIKPNQAIKEVAKRYQLKK
                     QEVYDIYHGLG"
     gene            26069..27160
                     /gene="serC"
                     /locus_tag="ACHU66_000580"
     CDS             26069..27160
                     /gene="serC"
                     /locus_tag="ACHU66_000580"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR3388887.1"
                     /translation="MTIYNFSAGPAVLPKPVLERAQAEFLDYNGSGMSVLEMSHRSKD
                     FDDIIKGAEATLRELMAIPDNYKVIFLQGGASLEFTMIPLNFAQGKKAYYLVGGSWGK
                     KAYTEAVKLSKTIAFEPILLGSTEDITYAELPTFDKNDIDPTAAYVHLTTNNTIEGTT
                     VYGIPDTNGVPVIADMSSNILAARYNVEDFAMIYAGAQKNIGPAGVTVVIVREDFLND
                     QPMLSSMLDYRIQAENDSLYNTPPAYSIYISKLVFEWVKEIGGVDEMEKINREKSGLL
                     YDYIDQSNFYTNPVRKKEERSVANIPFVSPSEELDAKFVKEATTAGFKNIKGHRSVGG
                     MRASLYNAFPHQGVVELIEFMKKFAAENA"
     gene            27160..27717
                     /locus_tag="ACHU66_000581"
     CDS             27160..27717
                     /locus_tag="ACHU66_000581"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR3388888.1"
                     /translation="MEIRLAHPNEMEAIVDILEDARAFLATSGINQWQGEYPNKENIF
                     DDILSGRGYVGIVDGKVAAYAAVHRGQEAEYEAIYEGKWQHNHPVYTTFHRIAAADEF
                     RGQGIIQTFLQGLIEGQKGPDFRCDTHEQNKTMQHILKKLGYVYCGKVPIDGERLAYQ
                     KIKQKSERSLYQEISEDDRWLLGNN"
     gene            28091..29272
                     /locus_tag="ACHU66_000582"
     CDS             28091..29272
                     /locus_tag="ACHU66_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR3388889.1"
                     /translation="MVFSVRTFNNINQIGLKELGNKFQIDGDHAANPDAFIIRSENLH
                     GFDFPENLKAIARAGAGTNNIPIEEATEKGIVVFNTPGANANAVKEAVIASILLSARD
                     YIGATAWANTLSGDDVPKQVEAGKKQFAGTEISGKTLGVIGLGAIGARIANDARRLGM
                     NVLGYDPYVSVETAWTISSHVKRVDDLKEIFTNADYITVHVPLTDKTRGLFNADSFGQ
                     MRKGTTLINFARGELVDNADLFEAIEAGVIKNYITDFGTEEVLNKDNIIVFPHVGGST
                     EEAELNCAIAAGQTIRRFMETGEIINSVNFPNVKQFLDAPYRITLINKNIPNMVATIT
                     TAVSELGINIDNIINKSKGDYAYTLLDLDEADKVKIEQLVAKFEATDAIVKVRVIQNK
                     N"
     gene            29275..29781
                     /locus_tag="ACHU66_000583"
     CDS             29275..29781
                     /locus_tag="ACHU66_000583"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR3388890.1"
                     /translation="MLYKQVYHSPLGPMSLIASDKGLRGAWFEGQKYFERGLNEKAIL
                     ASHPILDQTVRLLDQYFSGVQVDFSNLPLEAVGTNFQEHVWQLLKMIPIGQTTTYGQL
                     AQQLGLRSGQAVGGAVGRNPYSIIVPCHRVLNQKGQLTGYAGGLDKKIWLLRHENPEF
                     EVKDDFIL"
     gene            29765..30118
                     /locus_tag="ACHU66_000584"
     CDS             29765..30118
                     /locus_tag="ACHU66_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFR3388891.1"
                     /translation="MILFYEYPKCSTCRAAKAELKSLGLEFEAIDIKSTPPSAEELKA
                     WMEATGLELKKYFNTSGNSYRELGLKDKFDSLSLEQALNLLANDGMLIKRPLLIQDGK
                     ILQIGYRTKYENLGL"
     gene            complement(30135..31034)
                     /locus_tag="ACHU66_000585"
     CDS             complement(30135..31034)
                     /locus_tag="ACHU66_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HFR3388892.1"
                     /translation="MDHKIIERKSLTVSSVMNGLSGLAGIAVYIMTDLNALLLDGAFS
                     LIAFISSLVAVYISKNSHRKTETFPQGLYFLEPLYAIMKSLATLLLLVFAVLETSATA
                     FAYFVHGIGHQMTTGPVLPYTITMFFVCMGLYLYNRHMSTKVNHLSTIILAEAKGNLV
                     DGLISGAIGIAILLLYLVPINSWFGFLHYTGDFFLTLALAIISFKEPWNLLVASFKEL
                     ANGTIHFPEIHDSIYQLLEPYLKEDSNDVEIHIFKQGMKIRVKINLHNIHHEIIQKLL
                     EDKPQMLYLLKKHHEYISIEYAL"
     gene            complement(31334..32161)
                     /locus_tag="ACHU66_000586"
     CDS             complement(31334..32161)
                     /locus_tag="ACHU66_000586"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR3388893.1"
                     /translation="MKLISWNIDSLNAALTAESPRALLSRAVIDTLVTEDADIIAIQE
                     TKLSDKGPTKKHLEILESYFPGYANTWRSSVEPARKGYAGTLFLYKNHLSPTITFPEI
                     GAPTTMDSEGRIITLEFDDFYVTQVYTPNAGDGLKRLADRQIWDVQYADYLAKLDSHK
                     PVLATGDYNVAHKEIDLANPASNRQSPGFTDEERQGFTNLLAKGFTDTFRHLHGDVLN
                     AYTWWAQRSRTSKINNTGWRIDYWLVSDRIADKVSKSDMIDSGARQDHTPIVMEISF"
BASE COUNT         9596 a         5965 c         7220 g         9419 t
ORIGIN      
        1 gttttttatc taatctttct ctaaagttgg aatttacgtt atttttttgt gctatactgg
       61 cactacattg tctagaaaag ggcgaatatt gtataaagga gacatttcaa aaatgaaacg
      121 tattgtaaca aaacgtagtt ataaagttgg tctgtgtgct atggctcttg ctctgttgat
      181 gagcactgca ggtggcgtaa ctgcaggtgc acaagactat catgtcgaat catcaggcga
      241 attaaaacca atgagcgaag gagtgtttat gaaattggaa tcttcatcta atttcattga
      301 aaatgaagga atcgtggtga aatctattag taatcaaaat actaatccaa ataatttgaa
      361 cgagtcttgg tattggaaat tttggaagaa agtcaaactg tttttcaaaa gatttcgtga
      421 aggttttggt atacaaagat tatttggatt taaatagtca ataattaaag tgttattcaa
      481 atgttcataa tcttgtaata tttagcgcaa ttaatttata aaccttcata ttaaaaaaac
      541 taatactttg taaaaggaga catttcatga aacaagctaa ttcaaaacta gcagctttaa
      601 cactcattac aactcttgca gttacctctg ttattgggac aacggtgcat gctgaggaga
      661 aagtaactgc tgatcagact gtagcaggaa aacacaaacc gattacggat gagcagcttc
      721 aaaaagcgat tggagataga aatccaaata atattaagat acaagctaaa tttgaaaata
      781 ccaatttcaa aaatcaagga ataactttta ttggaaaagc tgaaattcca tctaaatctt
      841 ctgaaaactc ttcatggttt tggaatcttt ggactcgttt tacaactttt gtcaaaggta
      901 tttttagcta agatttctat ttttcgttga cttcatatgg atagagaaca gtggttcatg
      961 tgagccttta gtcaaatgaa aagcgtataa aatattggag agatctaggc atctaaacta
     1021 gagattgtgc aagaaattat gtaatagacc tcacattttt tgcgaatgag atgttgacac
     1081 gaattccata tttcttgagt ctcggttgta atttttctta gataaattaa agagtgaaaa
     1141 gtttaggatg taattgttct aaacttttta tattgcatcg ataaaaaaca cagtctttgt
     1201 gtaacaggta tattcttgta ttttttagtg aaaaaagaaa taaattgaca aaattatggt
     1261 aaaatatagg ttgatttaaa attaatcatc taatgaatgg aatgagttcg aagtaagtca
     1321 agattgacta gacattgtat ttaaagcaat tcgcaggaat aagagatgga aggagataga
     1381 acatgcaaca tccagttgta cgatttatct gtagaaccct gttctatttt tttatattgt
     1441 tactactttt gtatctgtat agttatttag acattggaca gggaggattt atctataatg
     1501 agttttaaag aggagagtat aaaaatgatt agcagtatgt tagagcgggt agagcatttt
     1561 gcgacagaaa atcctgacta ccctgtttac cattatctag gacagttgta tagttatggg
     1621 gatttaaagg cagattcaga ttctttagca gcccatattg atagtttgga tttaccagca
     1681 aactctcctg tccttgtttt tggcggacag gagtaccaga tgttggcaat ctttgttgcc
     1741 ttaaccaagg ctggccatcc gtatatccct gtagatagtc attctgcttt ggatcggatt
     1801 gaagctattc tggaaatagc agagccgagt ttggtgtttg cggtagcaga ctttccgcta
     1861 gagacaagtt tgccagtgct tggtttgtct gaagttgaag ccatttttga gaagaagtcg
     1921 acttatcagt tgatgcatcc tgtaagtgga gatgatacct actatattat cttcacttct
     1981 gggacaacgg gtaaaccaaa aggggtgcaa atttcgcatg acaatttgct cagcttcact
     2041 aactggatga taagggataa ggagtttgcg ctaccagagc gaccgcaaat gttggctcaa
     2101 ccttcctatt cgtttgactt gtcggttatg tattgggctc cgacgcttgc ccttggtggg
     2161 accttatttg ccctaccgaa agagttgact ttggatttta agaccttatt tgcagctatt
     2221 caaactttgc catttaagat ttggacttcg acaccatcat ttgtagatat ggctttgcta
     2281 tctcgtgagt ttgatgggga acatttacca gatttgacgc atttctattt tgacggagag
     2341 gagttaactg taaaaacagc tcagaagctc cgcgaacgtt ttccaaaagc tgtgattgtt
     2401 aacgcttatg gaccgaccga agctacagtc gccttatcag cagttgccat cactgatgag
     2461 atgctggtga attataaacg tttgccgatt ggctatgcca aaccagactc gccaactttt
     2521 attttggatg aggatggtca agtagtaccg aatggtcaac agggagaaat tgttgtatca
     2581 ggtcctgctg tctctaaagg atatttgaat aatcctgaga gaaccgaggc ggcatttttc
     2641 cagtttgacg gcttacaggc ctatcatact ggggatttgg gtgtaatgac agatgaaggt
     2701 ctattactct atggtgggcg tatggatttc caaattaaat ataatggtta tcgtatcgag
     2761 ttggaagaag ttgcccaaca cttaaataaa tcaacctata ttgaggctgc ggtagctgtt
     2821 ccacgttata atgctgacca taaagtgcaa aatctgttag cttatgttgt ccctaaagca
     2881 gggattatgg agcagttcga tcgaaagttg gagttaacca aggtcatcaa gcaggagtta
     2941 aaagaacgga tgatggatta tatgatgcct tcgaagttta tttatcgaga cagtcttcct
     3001 ttgactgcaa atgggaagat tgatattaag ggattgatcg ctgaggtgaa tgctcaatga
     3061 tggagttttt caaacagcta cctcagttag agccttatgg aaatccattg tactttttct
     3121 atctagtctt ggcgctagta ccaattttta taggactctt ttttaaaaaa cgtttcccct
     3181 tgtatgaaac aggtgttagt ttagccttca ttgtcttgat gtttacaggt acaaagacaa
     3241 tgcaattgct gtctttattg gcctatatta tttggcagac aatgttgatt tttttctaca
     3301 agcattatcg tcaacgggca aatcaaagct gggtcttcta tttaatcgtg tgtttagctg
     3361 tttttccgtt gacttgggta aagttggcac cgacttttag tcagcatggg gcaattttcg
     3421 gttttttagg tatttcttat ttaacgtttc ggtcggtcgg aatggtgatt gaaatgcggg
     3481 acggtttact aacggaattt agtttgggaa gttttttgcg atttttgcta tttatgccaa
     3541 ctatttcgag tggtcctatt gatcgttata gacggtttac ggaagattac aaacaaattc
     3601 ctgagcgaac tgagttatta aatatgctcg atcagactat gcattatatt atgatgggct
     3661 tcttatataa gtttattcta gcctatttta tcgggcatac tttactggag cctttaaagg
     3721 cagtagcctt ggatcaaggt ggatggttta acttacctac tattggagtt atgtatcttt
     3781 atggttttga attattcttt gactttgcag gctattccat gtttgccata ggtatttcca
     3841 atcttatggg tattcgctgc ccaattaact ttgatcaacc gttcaaatcg cgtgatctta
     3901 aggaattttg gaatcgatgg catatcagct tatctttttg gttccgtgat ttcgtcttca
     3961 tgcgtctggt taagactttg ttgaaacata aggttttcaa aaatcgaaat acggtatcta
     4021 atgttgccta tctgttaaat atgctcttga tgggcttgtg gcatggtgta acatggtatt
     4081 atattgcata tggtctcttt catgctttgg ggttgatcat caatgatgct tggattcgta
     4141 agaagaaatc aattaattta gctcgaaaaa aagctgggca agaaccattg ccggataatc
     4201 gttggacgag tttcgctggt atgtttgtga ccttccatac ggtcatgttc tctttcttga
     4261 tattttcagg atttttagat aaattgtggt tcaagtagac aataggtaac gattgtataa
     4321 atcgtagaaa ggaatataaa atggatgtaa aagaaacaat attgaatatt atcgaagaat
     4381 tatttatgga agatgtatct gaaatgatgg atgaagacct atttgatgcg ggtgttttgg
     4441 atagtatggg aactgtagaa cttattgtgg agttagaaag tcgttttaat attacagttc
     4501 ctgtttcaga gtttggtcgt gaagattgga atacagctaa caaaatcatt tctggtgttg
     4561 tggagttgat gcatgcgtaa gcaattgtgg cagatttttg ggcctgtgat ttgtgcaacg
     4621 tttttagtta ttctcacgtt gttgtttttc ccgagttctt ctcggtcaaa tttcaaagaa
     4681 gaaaaacaag atgcagtagc attgtcttat attagtttta agagtaaata taagaaaaat
     4741 agggctctgg gagacaaaaa tcatcgtttt gttcctttct ttgggtcaag tgaatggtca
     4801 cgttttgata gtatgcatcc gtcagtgttg gcggatgcat atcagagaga gtataccccc
     4861 tatttgttag gacaacgtgg tgcagcttcg ctgacacaat attttggcat gcaacagatt
     4921 ggggcacatt taaaaaatag tcaggctatc tatgttgttt ctcctcaatg gttttcaccc
     4981 aaaggggcat catctaaggc atttgaacaa tattttagta gtgatcaagc tactcatttt
     5041 ctgcaaacaa gtgctgggga tgactatgat agatatgcag caaagcgttt cttagagctt
     5101 aaatcagact gtagtttgac aggaatgttt caaaaagtag ctagtggtaa ggaattgtcg
     5161 aaatgggata gagagcagtt gacctggcaa gcctatttat tagaaaagca agatgcctta
     5221 tttagtcgct ttgctgattc tggtagtaat ttaggattgg tagaaacgga agcgatgaaa
     5281 cttccagaga ctttttccta tgaccgcttg agtaaagtgg ctaatgagga cgggagtcgt
     5341 tctactcaat caaatgattt ccaaattgat gataggtttt atagaaatcg tcttcagtca
     5401 aaaatcaaaa aactcaaaca atcccagaca aaaatatctt atctgcaatc accggagtac
     5461 aatgattttc agctggtttt gaatgaattt gctaaacatc agactgatgt gctatttgta
     5521 attcccccgg ttaatgaacg ttggatgaac tacactggtc tcaataaaga tatgtatcgg
     5581 gcaacagtag caaagattaa gtaccagttg accagtcaag gtttcaatca tattgctgat
     5641 ttgtcggagc agggggggat agattatttt atgcaagata caatccacat cggttggcaa
     5701 ggctggctgg agcttgataa gtatatccac ccattcttga cagaacaaca agctcctgtt
     5761 cattatcagc taaaaaaaga atttctaact tccgaatggg ctacatataa gggagattta
     5821 aacctatttt tgaaatagtt agatacagtt ataataaaaa gcaagaaggc tttgaattag
     5881 gaagtctcct tgcttttttg ttcttttact gtaataaact gaatgattat atatcttctc
     5941 ccaacaaact gtggttggct ttgtggaagc gaataacatg ataggcaatc atagcgtgtg
     6001 agagcaggtt taagataaca aatagtagga tatttgaatg accatccagc aataaggcaa
     6061 catttccaat tagtaaaatg agagcataga tgaacagccc tctcctgcca acttgatgga
     6121 cttgttgatt atagaaggag gtcgaaagga taccactata gaagagaagg tttcctacaa
     6181 tcagcatggg caaggccaaa tcattatgat gactagggaa aagctccatt gccactgtga
     6241 agaaattcaa gccaagaaca attaccaagt gagcgtatag cagaacagtc gcatctgcct
     6301 tgcggtgatg gtcgatagtg agataggtct gagaaatata gaagatgaac agagatgcca
     6361 ttgccaagaa gaggaggatt ccttccaaag gaagttctag aatcggataa tttttcaaaa
     6421 tcataatgac cgtctcacca aaggtgataa tagtaatcag ctggcagcgt tctacgatat
     6481 gaggaaagtt catcacatca tggctgagtt tgtctttgaa aaaataggaa atgaaaaatg
     6541 gaagataaaa aagcaagagg ctcttttcat cataggtcca gaaattgaag taaatagcta
     6601 ccgaagccaa gaaaaatact agcacataaa tcccaaacat atagagacta tgcttaatat
     6661 cagccgtaaa accgattttt cgaccccgaa ggcagataag acagggtcaa aaagatagta
     6721 aagggtaggg ctgtcgcttt aaaatcatga ctaaagttta gggataattg ccccaccaca
     6781 aacatagaag cgatgatagt gaaaatatcc agcaaatctc gctcaccata tttgttgagg
     6841 taaatcgtct cattaatcca caaggtcata ataatcaggt tagcggtgat aaaagttaga
     6901 atttccctga gtccgatatg gtccccgtgt aagaggccag tcagtgaaga ggtagctaat
     6961 acaaagacaa atcataaaat aattcatagt tggaaacacg ttttgctgta atttcagaca
     7021 taaaaatctc cttttcaaaa aaatctgacc tgttatacca tacgaaaagg gaggctgtca
     7081 tctcccttta tttcttacaa tgtccagtcc aagtctggtc cgactgggac aatacctgtt
     7141 gggttaatgg ttttgtggct accgtagtag tgctgtttga tatggtcaaa gtcaaccgtc
     7201 tccgcaatac cagagtggtt gtaaatccgc ttggtataat cccacaagtt tggataatca
     7261 accaaggcct tgatattgca tttgaaatgc ccaaagtaaa caggatcgaa tcgtaccaaa
     7321 gtggtaaaga ggcgaatatc agcctcggtc aattgattgc ccaccagata gtccttgtct
     7381 gccaaatgct cttccaattt atctaaggcg gcaaaaaggt tcttgacctc tttttcatag
     7441 actgcctgac tggttgaaaa acctgccttg tagactccgt tgttgatgtt tggatagata
     7501 aaatcattca tggcatcaat ctcggcttgc aggtttgctg gataatagtc atcgtaattt
     7561 ccagtaatat cattaaaagc agagttaaac atgcgcatga tttcagccga ttcattgctg
     7621 acgatagtat ttgtttcttt gtcccaaagg acaggcacgg tcacccgacc agaatagtgt
     7681 gaatcagcct tcaaatagac ctgatagaga taatcgctgt taaagagggt atccttgata
     7741 acaccaggac catcttcaaa tgtccagcca ttttctaaca tgaggggatg aacgacagac
     7801 aaggaaatat ggtcttccaa gcccttcaat tttcgcataa tcaaggtccg actagcccaa
     7861 ggacaagcca gagaaatata gaggtggtag cgaccagact cagccttgaa accgccctct
     7921 cctgttggac ctgcctgtcc gtctggcgta atccagttgc gaaattgtgt gacgctacga
     7981 ataaacttac cgcctgttga cttggtgtca taccattgat ccacccattt gccatcttgt
     8041 aaaagtccca tgaattttct cccttactga aaaaatggga aatccaatag gatgattgga
     8101 tttcctttgt aaaaaaatat atcactaact agtactataa acttttttca aaaagagtca
     8161 agctatatga tttgtgatac aatagtccca tgagattaga caaatgttta gagaaagcta
     8221 aagttggctc gcgtaagcaa gttaaaaaac ttttcaaagc ccagcaaatc aagattaacg
     8281 gacaagcagc tcaaagccta agccagattg ttgacccaga gttacaaact atccaagttt
     8341 cgggtaaaaa agttgcctta gaaggttccg cctattattt gctccataaa ccagcaggag
     8401 tcgtttctgc agtgacagac caggagcatc agacagtcat cgacttgatt tctccgcaag
     8461 acagtaggga aggtctctac ccagtcggtc gcctggacag ggacaccgaa ggcctggtcc
     8521 tcattaccaa caatggtccg ctcggttatc gcatgctcca tcccagccac catgtggaca
     8581 agttctacta tgtggaggta aatggatgct tggctgagga tgcttcaaaa ttttttgcct
     8641 caggtgttac tttcttggat ggaacacggt gtcagcctgc agttttgact gtcttggagg
     8701 cttctttgga ccatagcaga gcgaccatca agctggcaga aggtaagttt catcaggtca
     8761 aaaagatgtt tttagcctat ggtgtcaagg tcacttatct caagcggatt tcctttggcg
     8821 gttttgagtt gggggatttg gaacgaggtg catacagaca actcacacca aatgaaatgg
     8881 aacatttatt cacttatttt gactagagga ggatatataa tgagcattta tgattttaca
     8941 gtacaaaaac aagatggaac agatcagtcc atagctgagt atcaaggaca agttttactt
     9001 atagtcaata cagcgccagg ttgtggattg gcaccacaat acaaggaact acaggagcta
     9061 tatgatagct atcgaggcca aggttttgta gtgttggatt tcccttgtaa tcaattttta
     9121 aatcaggctc ccggctctgc ggaagacatc aatcagacct gtagcttgaa ttatggtaca
     9181 acttttcctc gttttgctaa aattgctgtg aatggatctg aagctagtcc gctgtatcgc
     9241 tatcttaaga aagaaaaatc aaccttatta ggaggtcgaa ttgagtggaa ttttaccaag
     9301 tttttggtgg atagacaagg gcgtgtggtc aaacgatatt taccaacaac tagtccatta
     9361 aaacttaaag aggatattga attttaccta gaaaaataac aagaaggctg gagtcatcta
     9421 gccttcttgt tttagtaacc acagaaaata gggagcgccg ataagggaga cgatgatgcc
     9481 ggttgggatg cctgttccga caagtagtac ccgtcctatg gtatccgcta gaagtagaat
     9541 gaagcttcct attatcatag tagctggaat gatgacacga tggtcatttc ctaaccattt
     9601 gcgcacaatg tgaccagcaa tgagaccaac gaaggtgata ttaccaacta aaatgacact
     9661 aagagaggct agggcggtag ccaatatcaa ggtaatccgc ctttcacgct gtaagtggag
     9721 tcccaaagcc atggctgtct ggtcgtttag attcataata ttgagtgagc gacttcgact
     9781 aaacgttgcc atccatacca atagaagaag aggagtatag attgcgatag ttttccaatc
     9841 tccaccattt actcgaccgc tgagccactg gacaatgtat tccatcttaa agtcatctaa
     9901 ttggctgatg atgctgacca tgataccaga cagcatgctc gaaattccaa ctcccgtgat
     9961 aattaagcgt gcaggatgga tgccgtacag cttcttacgt gcaatccagt aaactagaaa
    10021 aatggttaat ccaccgccca tcatagcaaa tagaggcatg agactcaata agacagggct
    10081 gatttttgaa gagagaccaa caaatagtgc cacaacaaga ccagctcctg cgttgattcc
    10141 taatattcca gaatctgcca gaggattgcg ggtcaaagtt tgtagcaaca aacccgccaa
    10201 agccagagaa gcccctccga taatggaggc gattattcta ggtaagcgaa ttcgcccaat
    10261 aatcagtaga gtcgatgaac tggcacgccc actcatgatg tcgatgagat ctgaaaagga
    10321 tgaattgcta tagccgatag ataaggaaag taggaaaatg acaaataaaa ggatcagcag
    10381 gaatgtgaat atttttagat agttgtgatg tcttatcaaa agtgtttctc ctttctaatc
    10441 aaccatagga agcaagggag accaatgata ctgatgactg cgctaatggg agtttcaaca
    10501 ggtggattga tagaacggca aaccaaatct acccagatga ggaaactagc ccctgcaaag
    10561 gcagttaatg gcaagagtag cttgtaattt ttccctgtaa acatgcggat aaagtgagga
    10621 ataatgagcc cgataaaaga aagcgagccc acaagagcta ccgcagaagc tgagagtaga
    10681 aggacaattc ccaacaaaac caaggtcatc agtaaggttc gttgacctag atttttagca
    10741 acggtttcgt tgagactcag gatggtcagc tgatgcgaaa agagttgagc aaggatgagg
    10801 ccgatgatga taagaggagc aatgatggcc agcatcttcc agtttacctg taccaatccc
    10861 cctgcttgcc agccaattac tgcagttgat aaatcaaagt agatagtaat agcctgacca
    10921 atagcagaaa atagacttgc taccatggcg ccagataaaa ttaaacggag ttgattgtag
    10981 cctttttgca cctgataagc tagaccaaag actagaccgg ctgccagaca ggagcccagt
    11041 aaacatatta ataaaatttg actgtagtgc aaaccaccga acagagcata ggcaacaatg
    11101 agagcaagtc ctgctccagc attgattcct aataagccag gatcggcaat cggattgcgt
    11161 gtaattcctt gcatcattgc cccagcttgg gccattgcag ctcccaccaa gatggcggct
    11221 accattctag gcaagcgcaa atcgatgata acatcctgag cggaagaatc tgtcagtgga
    11281 tgctttaggg tttctatcag ttgttggtgg ctataagaaa tagctccaaa gcgtaaacta
    11341 agatagactc cagctatgaa agaaagacag atgatgaaaa aaacaagcca aatattcttt
    11401 ggctttgttt taggataacg agaacttact agtttgttca tatatccagt ccttttactg
    11461 aagtttttga atttctgaga caaaggcgtc aatttgtaaa tctaaagaca tcggatcgga
    11521 gaagtagaag aggttatagt ctatctcgat gacgtgacca ttctttacag caggtagatt
    11581 tttccaaata tcactttctt tgagagaggc agctgcttct tttgttttat cattgacgtt
    11641 aacaaggaca tagtcgccaa catattctgg aagagcttct tgagataccc caaaccagcc
    11701 ttctttattg atgatgtctt cttgaacctt tgcaggagcg gcaaaaccga gagcgcgata
    11761 gattaattct ccaccacgac caaagttatt accataaagg aagatgttct tatcgaaaaa
    11821 gtccatgacg gtaaatgttg aacttgtatc aatcaatcct ttcaattcct ctttcgcagt
    11881 agcaactttc ttatcccatt gatctaacca ggcttgtgct tccttttctt ttccaaaaat
    11941 ctgaccaata tctgtcagca tttttaagta gtcactcttt ccataagtga tttcaataac
    12001 tggagcaatt tctttcagtg tttctaagtt atcatcacca gcaaatacta gaatgacatc
    12061 tggcttttgt gcggcaatcg cttctgtatc tgccgttgtc aattgtggaa catcctttag
    12121 tttgtctcca aagacgggac tatttttctc taaatctaga ctatagctag atacatcaat
    12181 tcctaattgt aagagatagc ctgtgtaaga gtaagcaaag ttgacaactt ttttaggatt
    12241 ttttggaatg tcaccgtggt aagtaatacc ttcaatcttt ggcatgctag acaattctac
    12301 gtctgttgaa gaggaagagg tagaacaagc tgttaagaaa atcagcccaa caaataaact
    12361 aaaaatagct aaaaactttt tcattttttc tccttattat tttttaaatc gtaagttagc
    12421 aaaatagggt aatcttgacc agcaacctgc atcaattgag gttcaatgct aaaaatgtcc
    12481 ctcagtatct cagttgtcat catattggac acagacccat ggtagcgaat atctccctct
    12541 ttcatggcaa tcatataatc tgaaaatcga gctgataaat tgacatcgtg aaggaccatc
    12601 acaatggttt tctgtcggct ttgattcagt tcttttaaaa gttctaagac ttccaattga
    12661 tgattgagat caagataggt tgttggttca tctaagaaaa tggtatccgt atcttgggca
    12721 agtgccatcg caatccaaac acgttgcctc tgaccacctg ataaatcatc aactggtaac
    12781 ctagcgtagg gagcggtctg ggtgacttcc aaggcccagt tgattttctc tctatcctga
    12841 tcagtgagat gccccagccc attttgatgc ggaaagcgac cataggagac cagttcataa
    12901 acggaaatcc cttctgtcgc ttctaagacc tgagggagca gggccaattt ttttgcaact
    12961 tctttggttg gcagttgtga aatggcttgt ccatctagat agatagcccc ttcttgaaca
    13021 ggaataatcc ttgtcaacgc cttaagcaag gtcgattttc cacagccatt ggcaccaatg
    13081 atcgtagtta tatttccttt tggaatactt gttgataatt catcaataat aatccgattg
    13141 tcgtaagaga cttggatatt ttccgcttga atcgaagaca caaactcctc ctttccagaa
    13201 tatgattcat taataatttg ttccgaccag tattatatca aaaaaattta ccaaaacaaa
    13261 gagttttctg aaaattttaa tttaatgaga atcgttataa aaaaagtcct tgtttgtgaa
    13321 caaggaccgt atgttacaaa tattgttttg ctacttccaa atcaccgtca taggcggcct
    13381 tttcgccgtt tacaatctgg taggcgtcag cccctttgcc tgcgatgatg acggcatctt
    13441 tcgtagagct tgtctgactc atggctgtgc gaatggcttc ttcccggtcc aagataaagt
    13501 tgcttggacg ggtcatgtgg gcacggattt gttcagcaat agcagctggg tcttcacggt
    13561 tggggtcatc agctgtcaga atcacctcaa tctgcggata gtcattgagc agaagaccga
    13621 agtctttgcg gcgactttct cccttgttac caggagctcc caaaatcaaa aataccttgc
    13681 ctgtctggtg ctctaagacg acatcaatca gcttcttgac actatcgccg ttatgggcgt
    13741 agtccacgaa taccttggca ccgttttgct gggttaggac ttccatgcga ccagggacat
    13801 tggtttgggc aatccctgtg tgaatatctt ccagacttgc tcccaagcgg agacaggcta
    13861 ggccggctgc gatggcattt tcttggttga agccaccaat cagctggatg tcgtaatggc
    13921 cagctaattt gccagtcgct gtgaagtcaa aacctgctga ttggctaatc tggttttcag
    13981 aagtcggccc ataaaagtca tggtctttgg agctgacttg ctctttcacg acttcaaaat
    14041 ggtccattcc tgcattgaca ataaccgctc tgctattgtc catcaaaaga cgcttatggt
    14101 agaagtagtc ctcaaaggtc ggatgctcaa tggggccgat gtggtcgggg ctgatgttga
    14161 ggaagacacc gacgtcaaag gtcagcccgt aaacccgctt gaccaagtaa gcctggctgg
    14221 acacctccat aatcaagtgg ctcatgccgt ttttaaccgc ctccgccatc atggcaaaga
    14281 ggtccaggct ttcgggcgtg gtcagggtgg acttgaagaa ggtcttaccg tccagtgtgg
    14341 tgttcatggt cgaaagcatg gcaggcttgt ggctctgttt gagaatgtta aaagcaaaat
    14401 aagccgccgt tgttttaccc ttggtgccgg tgaaggccag gagtttgagc tgtttctggg
    14461 gatgaccatg aaattccatg gcaatcaggc tcatagcctg cttgatgtca tggacgatga
    14521 tagcaggaat gtcaacctca tagtcgattt cagacacgta aaaagccagt ccggcctcga
    14581 tagctttttc cagaaattcc tttttaaagg ccaaaccctt ggcaaaaaag agggtcgtcg
    14641 ggctaaccgt gcggctgtca tagctgagag cgtcaaactg gacatccgtc catgtgctgt
    14701 cagtctgatc attctgtttg atatggcgga aattagcatc cgccttcaat atttctaata
    14761 cacgttcaat tgtaatcatg cttctattgt aaacctatcg aggatatttg acaagtctgg
    14821 gccatgtaaa tagtgggtat tttgaccgta aatttgaatg aatatgacat ttcgtactga
    14881 cgcaaatttg tgctataata ggagggattg aaaaggagaa gtttatgttt tattcttatc
    14941 tacgaacact attaacattc ttgttgtggg ccatcaatgg caatattcac tatcatgata
    15001 gagagaagat tttgccacag gaggaaaatt atattcttat cgctccgcac aaaaccttct
    15061 gggatccagt ctttcttggc tacgcagctg ctcctaagca gttcattttt atggctaaaa
    15121 aagaactctt caaggaccgc ggttttggtt ggtggatttc aaaatgtgga gcctttccaa
    15181 ttgaccgtga aaatcctggc atggcagcta tcaagtatcc agttaacatg ttgaaaaaaa
    15241 gtgaccgttc gctggtcatg tttccttctg gaagtcgtca ttcatctgag ctaaaaggtg
    15301 gtgtggcggt cattgccaag tcagccaagg tcaaactcat gccagcaacc tatgtgggtc
    15361 ccatgactat caaagggctc ttagctggtg agcgaatcga tgtggctttt ggaaatccta
    15421 tcgatgtttc ggacatcaag cgtatggatg atacaggtac agcagaagta actagccgaa
    15481 ttgaggctga gttcaaacgc ttagatgacc atgcggcatc tttccaaacc aagaaaaaac
    15541 caaatatctt gacttatatc tatcgtatcc cagttctttt gctggttgct cttatcttgg
    15601 gattgaccta tgcctttagc tatattgcca gcttcttctg gcaaccaagt actcagttgg
    15661 ataaaaaata agtaaatgaa actcgcagct tgcgggtttt tttgtatttt ttacgaacta
    15721 tataggtgga ggtaatttat ggatacgatt aaaacttata tagaattgct taaagaatac
    15781 aagtggcaaa ttgctctgcc agcagtggct ggcttgctaa tggcgacgtt cttaatattc
    15841 agccaaccag ccaagtctga tcagacgggt ctgactgatt ttccgcagac cgaacaaact
    15901 tcaagcggtc aggagcagac ggaggaaatc agtacagaag taagtgagga gcccagccag
    15961 ctggttgttg atgtcaaagg agcggtggat aaaccagggc tttacacctt agaagctggt
    16021 gcgcgtgtta atgatgcagt tgaagcagct ggcggcttga ccagtcaggc agacccaaag
    16081 tctatcaatc tggctcaaaa gctcagcgac gaggcggtgg tctatgtggc cagtaaggac
    16141 gaaaacatct cggtggtgac tagcacaact gccagctctg ctatgtcccc agaagaaaaa
    16201 agcaccagtc tggtcaatct gaatacggcg accgaggctg acttgcagac catttctggt
    16261 attggtgcca agcgagcgac ggacattatc gcctatcgtg aggccaatgg tggcttcaag
    16321 tcggtggacg acctcaacaa tgtgtcgggc atcggcgaca agactatgga aagcatccgg
    16381 ccttatgtca cggttgagta agctcccctg ccagcccgtt cacctggcag tcttggcggt
    16441 ggcggcctac tttgcagtcc attctttttc cctcttgaca atgggcctgt tcagtctgtt
    16501 actggctgtc tttggtgttc ggcaaggtaa gacggttttc ctcaaaacgc tgccgctttt
    16561 agctctatgt ggtctctttt tcggctacca aaaggtccaa tgggagcggg cagaccagtc
    16621 agccacagag caagtcacaa ctgtgcaggt cattccagat actattgaca ttaacgggga
    16681 cagcctgtct tttcgtggtc gggctgacgg acaaacctat caggttttct ataaattaac
    16741 cagtcaggag gaacagacct attttcaaaa gctgacaggt ttggtccagc ttgaagtgga
    16801 agcagagatc agtctgccag ctggtcagcg gaatttcaag ggttttgact atcaggccta
    16861 cctgaaaaca cagggtattt atcggacggt caagattacg gcaatcaaga agattgtccc
    16921 agtccagtct tggaatgtct ttgactggtt gtcaaactgg cggaggcagg ctttggttta
    16981 tgtcaaaacc aattttccgg ctcccatgag ccactacatg acagggctct tgtttgggga
    17041 cttggatagt gattttgacc agatgagtga cctctattcc agtttgggta tcattcatct
    17101 ctttgccctg tctggtatgc aggtgggctt tgtcatcgac aagtttcgct ggattttact
    17161 gcgtttgggc ttgaccaaag aaacggttga taaattacaa attccctttt cccttgtcta
    17221 tgcgagcttg acaggttttt ccgtatcggt ggtgcggtct ttggttcaaa aaattttggg
    17281 taatctgggc ttgcggaagt tggataactt tgcggtgaca gtttttgtct gtctgttgat
    17341 actgccccga tttctgctga cagctggtgg tgtcctgacc tttacctatg cttttttgct
    17401 gacggttttt gattttgagg acttggggca ggtcaaaaag gctgcggtgg aaagtctcag
    17461 tatttcactt gggattttgc cagtgctcat gacctatttc tatgcctttc agcccctgtc
    17521 tatcctcttg acctttgcct tttcttttgt ctttgatgtc ctactcttgc caggcttgtc
    17581 agtcattttt ctcctgtcgc caatcgtcaa gattacttgg gtcaacggat tttttgtctt
    17641 tatggaaaaa atcattgtct gggtggcgga tttgggattg cgaccttgga tactgggcaa
    17701 gccgtctgag ctggtccttt tgctcttgct ggtcagcctc ttcttactct acgatttcca
    17761 caggagaaag aaatggcttc taggactgag cttggtcctc gctttgctat ttttcataac
    17821 caaacacccg ctggaaaacg aggtgacggt ggtggacatc gggcaggggg acagcatctt
    17881 tttgcgggat atgcgggggc ggacggtcct gattgatgtg ggcggacgag ttgattttgc
    17941 ggccaaggaa gcgtggcagg agcggcctcg gcaggcaaat gcagagcgga ctttgattcc
    18001 ctatctgcat agtcggggtg tggataggat tgatagttta gtcctgaccc acaccgacac
    18061 agaccatgtg ggggatgtgc tggaagtggc gaagcaggtc cagattggtc ggatttacgt
    18121 gtcgccggga agcctgacgg tgcctgactt tgtagccacc ttgaaagaaa ttaatgtccc
    18181 tgttcatgtt gttgaggtgg gcgaacgctt accaattttt gactcttacc tggaagtcct
    18241 ttatccagat gggacaggag acggtggcaa taatgactcc attgtccttt atggtcgctt
    18301 gctgaaaact aattttctct ttacagggga cttggagcag ggggagttgg acttgataga
    18361 atcttatcct aacctgcctg tcgatgtcct caaagccggt caccacggct ccaaaggttc
    18421 ttcctatcct gaatttttgg accatatcga agccaagatt gccttggtat ctgctggaga
    18481 aaacaaccgc tacaagcatc ctcaccaaga aaccttggaa cgatttgata gtcgcaatat
    18541 ccaagtctat cgtacagacc agcaaggagc cattcgcttc cgaggttgga gggagtggag
    18601 gattgaaacg gtgaggaggt aaaatgataa atcctatgca cttatggttc tatagtttca
    18661 aagtttatgt gctcgctgcc ttatctggct atattctgca ctatgttact acgccagctt
    18721 ctgttaacga cttcaaagag gttgctgcct tattggaaag atgctaacaa tctgtgattg
    18781 tagcagattg ggctatctca gtcagcagtc ttgctcattc cattgtattt caaatagtct
    18841 atattttgaa tttaagaatt gattttcatt tcttttcatt aaatattgaa atcgttttcg
    18901 gatttgtgat ataataaagt atgatttacc aaaggaggaa gtcctatgtc tgttaaagat
    18961 tttatgaccc gtaaagttgt ttatatttca ccagatacaa ccattgcaca cgctgcagat
    19021 attatgcgtg aacaggattt gcaccgtcta cctgttattg aaaatgataa gttggttggt
    19081 ttggtcactg agggaacaat cgcagaagct agtccatcga aggcaactag cctatctatt
    19141 tacgagatga actacctttt gaataagact aaggtcaagg atgtgatgat taagaatgtt
    19201 atcacagtct caggttatgc tagtcttgaa gatgctgctt atctcatgta taaaaacaaa
    19261 gtggggattc ttccagttgt tgacaacggc caactatatg gggtcattac tgatcgtgat
    19321 atttttgcag cttttcttca tgtctcaggt tatggagaag aaggagtacg cgctcggttc
    19381 cttgttgaaa ataaggcagg ggagttagaa aaaattattc gattggtggc agataaggat
    19441 tataatattg tatctactgt gcaacttgct accaagtctg gaaaagttgt catcgaagtg
    19501 cagattgacg gaaaagttga tgttgaagaa gttcgaactc ttttcgaagg tgcaggtatc
    19561 caagtggata gcttggttcc gactgttgct aaaaatattt aaaaaccgag atttctcggt
    19621 ttttttcttt agctatcatt ctagtatttt aggggaatgt gatataataa agtatatcac
    19681 gtatgaggag gctctatggg gcacaaatat aaaaagtata agaaatatgg taaaaataat
    19741 tcaggacttg tggccctcct tgttttattg gcaatcggtt ttgtcatgag tttctggtat
    19801 atcttattgc ctactgggct cttaattggc tatttttaca agagagaggc tatccagcgt
    19861 tttttgaata gagattctat aaaacgctcg caggtattag cgctatctat tcgttcgggc
    19921 tatgaaaagc atcaagagct ctttgtggaa aagggtgagg ataaggttgt gttacagttg
    19981 agaaatagac tgttaggaca attatttgaa ttagatcagc tctatcaaaa agttggaaaa
    20041 tatctgaatg gatatgaaag taaagaagtt gtagatacct tgcaactaaa atatcattta
    20101 aaactgccag aagctaaaac agaaaaaaat actgacacac aggtttctac tggtggtaat
    20161 gctgtgatgg atgaacaagg catgatagcg gatttagctc ctgaaattct agaaacctat
    20221 tgtaatattc aaagagacaa tcttgttatt cttgaaaagc tggaaaaggc gacagataag
    20281 agggaagaat tgacagctat ccatgaggcc aatatgaatc ggtttaacga tattttacaa
    20341 ggttatttga aaatcaaaaa atcaccgaaa gattatttta acgcagaaga acgattggtg
    20401 caggcaaaat cagccatgga aatgtttgat aaggatttgg atgataccat caaacaattt
    20461 aacgaagcgg gtatgaaaga ttttgaagtc agtctgcgta tgatgaaaag agaagaggaa
    20521 tagagaagat gtcaggattt aatttcgata ttgatcagat tgctaataat agtttatcaa
    20581 ccaaagataa gacaacagag cttattcaag cgactccgac tggagataat tcaaaagtgt
    20641 ctttcttggc acagttgaca ccggaacaac aaacaggtat tcgtgctaag gctccccaat
    20701 tggtggataa tttcttagct aatcaaaatg ccctcttgga ttttggtaag gaagcggtag
    20761 aagaagtcaa tgccacggtc aatcacattt tgtcagaaca gaagaagatt gaaattccac
    20821 aggtggatga gcttttggcc aataccaacc gtgaattgaa tggttttgtg gctaagtata
    20881 aggacatttc cacaactgct gaattagaaa agaaaccagg tttcttccaa cgcctgttca
    20941 agcagaccaa aaatgatttg caggaatttt atttcgactc gcagaccatt gaaaagaaaa
    21001 tggatagcat ggcagccagt gtggtcaagc aggaagaggt gttggcgcgc aatattgttt
    21061 ctgcagagct tttgattgaa aataatacca agtctatcga gaatttggtg ggggtcatcg
    21121 cctttattga agcgactggt caggaagcgg cagagcgtgc aaaattggca caagaaaacc
    21181 tcgcaacttt agtaccgact acagtcgatt atcaagtagc ttctgagaag ttggcacgtg
    21241 taacagaggt tgctaatatc ctcgaacaac aacattctga gtatatgagt cgcttgtatg
    21301 ttgcgtggac aacgacaccg cagatgcgta atctggtcaa ggtttcttcg gatatgaaac
    21361 agcgccttgg gatgttacgt cgcaatacta ttccaacaat gaaattgtcc attgcacagt
    21421 tgggcatctt acaacaatct atcaagtcta gccagacggc agatgcgatt gtcaatgcta
    21481 ataatgctgc ccttcaaatg cttgcggata catcaaaaga agccatcccg atgatggaac
    21541 gcacagctca gaatccgacc ctttcagtag catctgttac caaattggca gaaagtttgg
    21601 ttgcccaaaa taatggcatt attgcagcta tagatgaagg gcgtcagaaa cgtcgtgagt
    21661 tggaggcagc tattgtacgg tcagcagaga ctatcaacga ttctgttaag ctacgcgatc
    21721 aaaaaatcat tgctgctttg ctggagcaag gcaaagaagc acagtctgag gcagagcaac
    21781 cacttgaaac caatcaataa tataaaacct gcagttcagc gaaactgcag gtttgtttaa
    21841 tggcgttcta ttggtttttt atctcgatct aaagtaaatc cctcaccgag tacttcatgt
    21901 gcgtctgtaa tggtgataaa tgcgtggggg tcaatacgat gaataagctc cttcatctct
    21961 tggagttggc tcttatatat gaccgagtaa atcatgttga catcggcttt gctgtagaaa
    22021 ccttgtgctt tgataaaggt gacaccacgc tcgatttcac aatcaatagc ctgagccaat
    22081 tggtcagatt tttgagaaac aatcataacc cccttgctac catatccgcc ctcagtgaca
    22141 aaatcaataa ccttggaagc aatagcaacc atcattaaag tatatagaac tgttcggagg
    22201 tctttaaaaa caataacaat cagagttagg atgaggatgt caactgcaag aatgatttgt
    22261 ccaatggagt aaggtaggaa tttatgacct atacgagcaa tgatatcgct tcccccagtc
    22321 gttccgccag cacggaagat ggttcctaat cctagtccca taagaatccc acccaggata
    22381 ctaacgagta tcaaatcttg ttgtagattg attgaaaaat ggattttttc aaaaatggcc
    22441 aaccaaaagg taacgctaaa ggttcctaag atactgagat agagagtctt ctttccgagt
    22501 attttccacc cgaggatgaa caatggaata ttaatcacaa tgttcattat ccaaggttga
    22561 atatggaaaa ggtagtaaat aatcagcgtc aatccagttg cgccaccctc gaacagacga
    22621 ttgggcatga ttaaataatt tagcccaaag gcaaatagcc ctgcaccaag caaaataagt
    22681 aaaatatttt tcaaacgaac cataagcatc tccgaatatg tgtatatatc aattttaaaa
    22741 gattttctcc ttaaaaacaa gtcaattttc aagtgatttt ttgactaatt tttggtaaaa
    22801 tggaatgaat atctaagaac cagtcttcac aagtgaagtg ctgaactgtg catactggta
    22861 ctaagagcga gggaaagatg agaaacggtt tttttattac atttgaaggt ccagatgggg
    22921 cagggaaaac gacggtattg caacagttat tgccagcctt gcaggaattg gggccagaag
    22981 tagtcacaac tagagagcca ggtggggtgg ctattgcaga agaaattcgc aatattattc
    23041 tgaaccctgc aaatactgaa atggatgata agacggagct gttattgttc attgcagcac
    23101 gacgtcagca tttgaaagag aagattcttc cacccttggc acaggggaaa ttgctcttga
    23161 ttgatcgttt tatcgattcc tctattgcct atcaaggatt tggacggggg ttggatatag
    23221 gtgacattca ttggctcaac cagtttgcaa cagatggttt aaagccagat ttaaccctct
    23281 attttgatat tgatacagaa gaggggctgg ctcgaattgc ccgcaatgct gaccgtgatg
    23341 tggatcgctt ggatatggaa aaagcagata tgcatagacg ggtgcgtcaa ggctatctta
    23401 atattttaga gcaggaacca gagcggtttg taaagattga tgccagtcag ccactagagg
    23461 ctgttgttgc agatgctctg gcggtcatta agaaacggtt tgcagaaaga acatgaagat
    23521 tgaagaactt agaaacttac aacccaatgt atttcaacgt tttgtgacaa ttttagagca
    23581 gagacgtttg gtgcatgcct atctattttc aggtgacttt gctagttttg atatggcaat
    23641 ttttctcagt caatcgttat tttgtcagga gaaagaaggc gtccttcctt gtcagaagtg
    23701 tcggtcttgt cgtctaattg aagcaaatga gttttcagat cttactgtta ttgcaccgca
    23761 gggaaatgtt atcaaaacag atacgatacg tgaattagtt aagaattttt cacaatctgg
    23821 atttgaatcc aataagcagg ttttcattat tcgtgatgct gagaaaatgc atgctaacgc
    23881 agccaactct ttgctaaaag tgattgaaga acctcagtca gctattcaca tcttattatt
    23941 gaccaatcag gaagaagctg tccttcctac tatcaagagt cggacacaga ttataggttt
    24001 tcctaaaaat attccggcca tggagcgaat gttagaagag gagggcttgt taaaaacaca
    24061 agcgaatctc ttagctcaac tggtttcaag tcaagaagaa gcgaaaaagt tagcagaaaa
    24121 taaaaacttt ttagatttga tgggacagac aaagaaattt acagacttgc tcttggtgga
    24181 gcctacacgt gcttatttgc aagttggaat cctagtgtct ttagctgtgg aaaaagctga
    24241 gcagggacgt ttgtttgacc ttctgagtct gatattatct gagaaaatga tggaatcggc
    24301 aaacgtacgt gaaaaaatgg atgctgttct taaggctaag aagatgtggc aggcaaacgt
    24361 aagtctacaa aatagtctag agtatataac tctgtaaaat aaaagaaagg gagataatgg
    24421 ataaaaaaga aatttttgat gccctagatg atttttcgca gaatcttcta accacgttgg
    24481 cagaagtgga tgctatcaaa aagcatttac agggagttat tgatgaaaat acgaccctcc
    24541 gcttggaaaa ttccaagttg cgcgaacgtt tggaaaaaga agataagaca ggccataaat
    24601 cttccaactt tggtaaggag aatttggaga atatttacga agatggtttc catatttgta
    24661 ccttctctta tggtcaacgt agggataatg atgaaccttg tatgttctgt gtagaattat
    24721 tgaatcgaga ttaggatgaa agtacaaaaa tcatttaagg gacagacgag ttttgggact
    24781 ttatatctgg tgccaacgcc tattggaaac ttacaggata tgacattccg agctgttcag
    24841 attttgaaag aagtggatat aattgcagcg gaagatacaa gaaatacagg actcctactc
    24901 aaacattttg aagtagaaac caagcaaata tcctttcatg aacacaatgc ccatgaaaaa
    24961 attccagttt tgattgattg gctcaaatct ggtcagtcca ttgcgcaagt ttctgatgca
    25021 ggcctgcctt ccatttcgga tcctggtcat gacctggtag gggcagcgat tgcggaagat
    25081 attcctgttg tagcacttcc aggagcatcg gcaggaatta cagccttgat tgcatctggt
    25141 ttggcacccc agccacatat tttttatgga tttttaccga gaaaatcggg gcagcaaaaa
    25201 gaatttttcc aagaaaaaaa gacctatcct gagacgcaaa ttttctatga atctccatat
    25261 cgtgtggcag ataccttaga aaatatgttg acagtttatg gtgatcgtca ggtaacagtt
    25321 gttcgagaat tgaccaagtt atacgaagaa taccagagag gaagcatttc cgaattacta
    25381 gagtatttgg aagaaaatcc acttaaggga gaatgcttga ttattgtcga gggtgctggt
    25441 gaggaagtct atccttctgc tgacgaagta gacttgaagg ctgaggtaga aagggaaatt
    25501 gcaagtggca taaaacctaa tcaggctatc aaagaagtgg ccaaacgcta ccaactcaaa
    25561 aaacaagaag tatacgatat atatcatgga ctaggttaag acctagtttt tttgtatgtt
    25621 tatgcaagct tatactcttc gaaaatcaaa attatccgtt gtcaagagcc ttgatgagtg
    25681 aaaagctcca gtggatcttt tcagtctgtt accttgaaat gagaaaggaa cagagttcta
    25741 tcttcggctt aatgacgcga acacagttct ctatacttcc agccttcaac tatatcccag
    25801 ctaagctact tttgattttc attgagtatt aatatgcaga tagcaattgg cgacactccc
    25861 caaacagtca aatctggtca taaaattttc attgaaacct gatgaatccc tgtgaggcaa
    25921 gcgcctcgtt tatggctaaa agtaagaggt tctttagaaa gtagaataaa tataagaaat
    25981 ccgaatgttt ttacaaaata atcatatttt atggaattgt ctgaatattt gtgatataat
    26041 gaacagacta acttaatgga gggattttat gacaatctac aacttctctg caggtcctgc
    26101 agtattgcca aaaccagtac ttgaacgcgc ccaagctgaa ttcttggact acaatggttc
    26161 gggaatgagc gttttggaga tgtcgcatcg ctccaaggac ttcgatgata ttattaaagg
    26221 tgctgaggca acgcttcgtg aattgatggc cattccagat aattacaagg ttattttctt
    26281 gcaaggtgga gcttctttgg aattcacaat gattcctctc aacttcgctc aaggtaagaa
    26341 agcctactat cttgtaggtg gctcatgggg taaaaaagcc tatactgagg ctgtgaagct
    26401 gtctaaaaca attgcctttg aaccaatttt gcttggatca acggaagata ttacctatgc
    26461 agaattgcca accttcgata aaaatgatat tgatccaact gctgcttacg tgcatttgac
    26521 aacaaataac actattgaag gaacaacagt ttatggtatt cctgacacca atggtgtgcc
    26581 agtcattgcc gacatgtctt caaacatctt agcagctcgc tataatgttg aagattttgc
    26641 catgatttat gctggcgctc agaaaaatat tggtcctgct ggtgtgacgg tggtgattgt
    26701 tcgtgaggac ttcctcaatg atcaaccaat gctttcaagc atgttggact accgtattca
    26761 agcagaaaat gactctcttt ataatacgcc accagcttat tctatctata tttctaaact
    26821 cgtctttgaa tgggtaaaag aaattggtgg tgttgatgag atggaaaaaa tcaaccgtga
    26881 aaaatctggt ttgctttatg actatatcga ccagtctaat ttctatacaa accctgttcg
    26941 caaaaaagaa gagcgttcag tagcgaacat tccttttgtc tcaccaagtg aagaattaga
    27001 tgccaagttt gtcaaagaag caacaacagc tggttttaaa aacatcaaag gtcaccgttc
    27061 agtaggtggg atgcgggcgt cgctctataa tgctttccca catcaaggtg tggttgaatt
    27121 gattgaattc atgaaaaaat ttgcagcgga gaatgcttaa tggaaattag attagcacat
    27181 ccaaatgaga tggaggctat tgtcgatatt cttgaagatg ctcgtgcatt tctagcaacc
    27241 tctggtatca atcaatggca gggagagtat cctaataaag agaatatctt tgatgacatt
    27301 ctttctggca gaggttacgt tggtatagtt gacggtaagg tggcagccta tgccgccgtt
    27361 catcgtggtc aagaagcaga atatgaggcc atatatgaag gaaaatggca gcacaatcat
    27421 cctgtatata caacatttca tagaattgca gcagctgacg aatttcgtgg tcaaggaatc
    27481 atacaaactt ttttgcaagg tttaattgaa ggtcaaaaag gaccggattt ccgctgtgat
    27541 acccacgagc agaataagac aatgcagcac attctaaaaa aattaggcta tgtctattgt
    27601 ggtaaagtcc caatagatgg agaacgtcta gcctatcaaa aaatcaagca aaagagcgag
    27661 cggagtcttt accaggaaat cagtgaagac gaccgttggt tactcggaaa taattaatga
    27721 aataaagaaa gaaaaggacc gggaaaaagc ttcatatcac ttctcaaggt tcgagtcaac
    27781 atctcagcac agtggttgat tgggtttaac agtccagtgg actgttaaag gttggagata
    27841 agatttgcga agcaaatctc agcagttcgt attttatact ccaaatctga cctctacgac
    27901 tgatgcgaac agagttcacc ttatctccaa cctcaaactg tctcccagac agtttgagct
    27961 gtgcgggggt gggagtgaaa cagtctggga atagactgtt tcagctcaac aaatagaaat
    28021 aaagacttgt tgacgaactc ttctaggagt ggttgagttc tttcccactc ccctttttag
    28081 gtaaaaataa atggtattta gcgtacgtac ttttaacaat atcaatcaaa ttggtcttaa
    28141 ggaattaggc aataaattcc agattgacgg agatcatgca gcaaatccag atgcctttat
    28201 tattcgatct gaaaatttgc atggatttga ttttcctgaa aatctaaaag ctattgcccg
    28261 tgctggtgca gggactaaca atattccaat cgaagaggca acagagaaag gtattgttgt
    28321 ctttaacact ccaggtgcca atgccaatgc cgtaaaagag gcagtcattg cttccattct
    28381 tttgtcagca cgtgactata tcggagcgac tgcatgggca aatacattgt caggagatga
    28441 tgtacctaag caggttgaag caggtaagaa gcagtttgct gggacagaaa tttcaggtaa
    28501 gactcttggt gttatcggac ttggtgctat cggtgcgcgt attgccaacg atgcccgccg
    28561 tttggggatg aatgttctcg gttacgaccc ttatgtatca gtagaaactg catggaccat
    28621 ttcaagccat gtcaaacgtg tggatgactt gaaagaaatc tttaccaatg cggattacat
    28681 tactgttcat gttcctttga cagataagac acgaggactc ttcaatgcgg atagctttgg
    28741 tcaaatgaga aaaggaacaa ccttgataaa ctttgcccga ggtgaattgg ttgataatgc
    28801 tgatttgttt gaagcaattg aggcaggagt tatcaagaac tatatcacgg attttgggac
    28861 agaagaagtg ctcaacaagg acaacattat cgtcttcccg cacgttggtg ggtcaacaga
    28921 ggaagcagag ctcaactgtg ccattgctgc tggtcagacc attcgtcgtt ttatggagac
    28981 gggtgaaatt atcaactcag ttaacttccc aaatgtgaaa caattcctag atgcaccgta
    29041 tcgtattacc ttgattaata agaatattcc taatatggtg gcaacgatca caacggctgt
    29101 atctgagttg ggaatcaata tcgataatat catcaataag tctaaaggtg actatgccta
    29161 tactttactt gatttggatg aagcggataa agtgaaaatt gagcagttgg ttgctaaatt
    29221 tgaagcaacg gatgcaattg tcaaagtgcg tgtcattcag aataaaaact aatcatgctg
    29281 tataaacaag tctatcacag tccacttggc cccatgtcat taattgctag tgataaggga
    29341 ttgagagggg cctggtttga gggacaaaaa tattttgaac gaggcttgaa tgagaaagct
    29401 attttagcct cccatcccat tttagaccag acagttcgat tgcttgacca gtatttttca
    29461 ggggttcagg tggacttttc aaaccttcca ttagaagctg tgggaacaaa tttccaagag
    29521 catgtctggc aattattgaa gatgattccg attggtcaga cgactaccta tggacaactg
    29581 gctcagcaac tgggccttcg ttctggtcag gcagtaggag gagcagttgg gcgtaatcct
    29641 tattcgatta ttgttccttg tcaccgagtt ctcaatcaga aaggacagtt gacaggttat
    29701 gcaggtggac tggataagaa aatctggctc ttgcgacatg agaatccaga atttgaggtg
    29761 aaagatgatt ttattttatg agtatccaaa atgttcgact tgccgagcag caaaggcgga
    29821 gctgaagagt ttgggattgg aatttgaagc tattgacatt aagtcaacac cgcctagtgc
    29881 tgaagagcta aaagcttgga tggaggcgac aggactagaa ttgaaaaagt attttaatac
    29941 ttctgggaat agctatcgtg aactaggttt gaaagataag tttgatagtc ttagcttgga
    30001 gcaggcactt aatcttctag ctaatgatgg tatgctgatt aagcgtcccc tactaattca
    30061 ggatggtaaa atcttacaga ttggctatcg aacaaaatac gaaaatctcg gtttataagc
    30121 cgagattttg tttattatag cgcatattct attgaaatat attcatgatg ttttttcaaa
    30181 agatagagca tctgaggctt atcttctaag agtttttgga taatttcgtg atggatattg
    30241 tggagattga ttttaacgcg aatcttcatg ccttgtttga aaatatggat ttcgacatca
    30301 ttgctatctt cctttaagta aggctccaat agctgataga tagaatcatg tatctcaggg
    30361 aaatggattg ttccgttggc aagttccttg aaagatgcca ctaataagtt ccaaggttcc
    30421 ttaaatgaaa tgattgctag tgctagtgtc aaaaagaagt caccagtata atgcaggaag
    30481 ccgaaccaag agttgatagg aacaagatag agcagaagaa ttgcaattcc aattgcacct
    30541 gaaatgagtc cgtctacaag gttgcctttt gcttctgcta atatgatagt agacagatga
    30601 ttgactttgg tactcatatg gcggttgtat aggtaaagcc ccatacagac aaaaaacatg
    30661 gttatggtat aagggagaac gggacctgta gtcatttgat gaccgattcc atgaacaaag
    30721 taagcaaagg ctgtcgcgct agtttcaagt acagcaaata caaggagtag tagagttgcg
    30781 agagatttca tgatagcgta gagaggttct agaaaataca gtccttgagg aaaagtctcg
    30841 gttttccgat ggctattttt tgatatgtaa actgcaacta aggacgaaat aaatgctatt
    30901 agagaaaagg ctccgtctag gaggagggcg tttaggtcag tcatgatata aacggcaata
    30961 cctgctagtc cagataaacc gttcataaca gaggaaacag ttagagattt gcgttcgata
    31021 attttgtggt ccataatccc tccttttaat gtatatctat atatttattt taccacaaaa
    31081 tgacaccgtt ttcaaattga gcggattaaa acaaggtaga gaggtagagt gataagaaat
    31141 aaaggctcaa aacccttgca atagacctac gggttttaag cctttaatat ctgttatctg
    31201 ataattgaaa tcaggttata ctcttcgaaa atcaaaatca gacgttgttg acttgatttg
    31261 atgaacttca gttctatctg catctgcgtc gcctagtctg attttgattt tcattgagta
    31321 ttaatatcca ctgttagaat gaaatctcca tcacaattgg tgtatggtcc tgacgagcac
    31381 cggagtcaat catatctgac ttacttactt tgtcggcgat gcggtcgctg acaagccagt
    31441 aatcaattct ccagcctgtg ttgttgattt tactggtgcg gctacgttgt gcccaccaag
    31501 tataggcgtt gagaacatca ccgtgcaagt ggcggaaggt atctgtaaaa ccttttgcta
    31561 gaagatttgt aaaaccttga cgttcttcat ctgtaaaccc tggagattga cggttacttg
    31621 cgggattagc aaggtcaatt tccttgtgag caacgttgta gtcacctgtt gcaagaacag
    31681 gtttgtggct atcaagtttt gctaagtagt cagcatactg tacatcccag atttgacggt
    31741 cagcaagccg tttcaagcca tcaccagcat ttggcgtata aacttgtgtg acatagaagt
    31801 catcaaattc tagcgtgatg atacgccctt cagaatccat ggttgttgga gcaccaatct
    31861 cagggaaggt tatggttgga cttagatggt ttttgtagag gaagagtgta ccagcatagc
    31921 ctttgcgagc tggttctaca gatgaacgcc aggtattggc atatccaggg aagtagcttt
    31981 ccaaaatttc tagatgcttt ttagtcggtc ccttatctga caattttgtt tcctgaatgg
    32041 cgataatatc agcgtcttct gtcacaagtg tatcgatgac tgcacgtgat aatagggcgc
    32101 gaggagactc tgcagtcaat gcagcgttaa gagaatcaat attccaagaa atgagtttca
    32161 taattttcct tttctagtct gttactgttt tacattatac
//
LOCUS       DAZQCJ010000014        29718 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000014 DAZQCJ010000000
VERSION     DAZQCJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29718
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.014"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            55..1923
                     /locus_tag="ACHU66_000587"
     CDS             55..1923
                     /locus_tag="ACHU66_000587"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFR3388894.1"
                     /translation="MTLVTNLKQHSNIITTALCLALILIGILLFQTGQGWAPILFISA
                     FVIGGYQSAKEGISELIFDKHLSVDLLMILAAIGSGLIGYWMEGALLIFIFSLSSTLE
                     ELAMEKSKNAIAALMNMTPPTARKIEENGDITVLDTAAIRIGDLLQVRKGDTVPLDAT
                     LISPQSIFDESMITGEPLPAEKMTGAAVIGGTINQGPTVTVQVTAEKGDALFDKIVQM
                     VENAQESKSKTATFIENMEDTYVKIVLVVVPLFILFAHFALCWDWLTAFYRGMILLTI
                     ASPCALVASSSPATLSAISRAARKGMIIKGGDIADNIANLEAIVFDKTGTLTIGKPEV
                     VDATYLGDEKLIREVVQAVEKQSSHPIAQALMTYTADSSAIALQSLEDVTGKGLVAGY
                     EGDSWKIGKAGFVVDSLVSPLSAGLLAQIDEAESTGKTLVYVSQNDVLVAIFMVEDSL
                     KPESKQLIAQLKEMGVTPILLTGDQEKTARYVASQVGIDRVIANCLPTDKAAVIQKLQ
                     TEFASVGMVGDGINDAPALAQANVSYAMGSGTDIAMESADIVLMEDLTRIPYSIRLSK
                     KMRGIIKQNIIFALSVIALLIVSNLFQSINLPLGVVGHEGSTILVILNGLRLLYFK"
     gene            2058..2582
                     /locus_tag="ACHU66_000588"
     CDS             2058..2582
                     /locus_tag="ACHU66_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388895.1"
                     /translation="MKTNLLKTAGLLAGCVRYFFYLLIAGYIFFSLAGFRGDSFVIGS
                     WEYTFNKPIDQISVWLILLTSGLILIILAVLSHIAHLIQKLCKLLLEEDYFASASLEL
                     YQKLFISLLVLTAGQFCLTSLITLTNAAGPNNFLNLRWSDFILNALFIFLTYFVWTLV
                     QKGQQLETENSEFI"
     gene            2584..2799
                     /locus_tag="ACHU66_000589"
     CDS             2584..2799
                     /locus_tag="ACHU66_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR3388896.1"
                     /translation="MEMIRVNLDKVLKDRQMTSKDLAEQVGITEANLSILKTGKAKGI
                     RFNTLMSICRILDCQPGDILEYVKEGE"
     gene            2801..3307
                     /locus_tag="ACHU66_000590"
     CDS             2801..3307
                     /locus_tag="ACHU66_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFR3388897.1"
                     /translation="MNKKIFFNIVIQLLTFIRHGLIALTGLIMLALLILLIGGKPIQN
                     SLQLDVPLPIIVIILIISILLVACFIYMATILKKLLINFQTGTIFSTENVVYTKIILG
                     TLIIWTSIQFASAFFLSYLDLENVSDLYDFSLSDYFINGVLIIIALLAKMVLEKGVLL
                     QAENDEII"
     gene            3319..3789
                     /locus_tag="ACHU66_000591"
     CDS             3319..3789
                     /locus_tag="ACHU66_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388898.1"
                     /translation="MKKYISLIALCMMIPCLSACHSVTSEVNKEMQNELTAASIETEK
                     ALKTDERLAEIESFQQIVEIAASKGEFSLESSFSGKVYLMATKENLEKLSKEEFTLNK
                     KSFSTIDAGSSNDFNIRYKHQTYFTVQSLSVQDMEDFEYTKSSSTSAILVFYPN"
     gene            complement(3826..4497)
                     /locus_tag="ACHU66_000592"
     CDS             complement(3826..4497)
                     /locus_tag="ACHU66_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR3388899.1"
                     /translation="MDINWQAVFNADIAREAIPQILEGLPYTLSLSLIGFALGTFCGF
                     FVAFMRMSKFGPLRWLAMAHISLMRGIPLMVLLFFIYFGLPFMGLQLDAISASIIAFT
                     SMSSAYISEIIRASLSAIDKGQWEAARSLGLRTNVIYRKIIIPQAFRIALPPLSNVLL
                     DMVKSTSLTAMITVPEIFNKAKIVGGAKSDYMTVYICVALIYWAICTLYAFGQLRLEK
                     RLATY"
     gene            complement(4588..5463)
                     /locus_tag="ACHU66_000593"
     CDS             complement(4588..5463)
                     /locus_tag="ACHU66_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFR3388900.1"
                     /translation="MLKKVLASALLVSTLTLAACSSSSSNTSSSSAEQTQWEKVQEAG
                     VLKVATPGTLFPTSYYNDAKELVGYEIDMINEIGKRLKLEIEYQEIGVAEAFTAVDSG
                     KVDISVNNFDMTEARKEKYNFSIPYKYSVGGYIVREDGSSGIEAADLSDWTGKKAGGG
                     AGTQYMKIAEKQGAEPVIYDNVTNDVYLRDVSTGRTDFIPNDYYTQVMAIKWANANFP
                     DIKVKMGDAKYNPTEQGIVMSKADTSLKEKIDEALEAMKADGTLKAISEKYYAGQDLT
                     VPVENADQLPVIEVE"
     gene            complement(5588..7036)
                     /locus_tag="ACHU66_000594"
     CDS             complement(5588..7036)
                     /locus_tag="ACHU66_000594"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFR3388901.1"
                     /translation="MTWKDATAMAQAVNQKQVSAKELVQETIDRIEKLNPTLNAVVSK
                     QYEEALKEAEKEDYLGKPFAGVPFLLKDLGQNEKGQPSSAGSRLLAGRPAGHTDTYVQ
                     RLKDLGFIIVGRTNTPEFGFKNISDASLHGPVKLPLDPSRNAGGSSGGAAAALASGMV
                     SIAAASDGGGSIRIPASFNGLIGLKTSRGRIPVGPKSYRGWQGASVNFALTKSVRDTQ
                     RLLYHFQDYQLEAPFPLAKLSEEEVFGKLSRPLKIAYYTKSPVGSKVSPEAVEAVQKA
                     CHHLANLGHEVVELSEYPLDGVALMKSFYLMNSVDTAQMFDEIEAAFGRQMTLDDMEV
                     MSWAIYQSGQTIPAKLHSKALLDWDQFGASMARFHEEYDLLLTPTVADVAPKHGQFDL
                     SADLLDRLKQTQNYSMEEQQDLIWQMFEDSLALTPFTQQANICGQPAISLPTYVRADG
                     LPIGIQLTASKGREDLLLRLADQMEAAGLLNL"
     gene            complement(7046..7873)
                     /locus_tag="ACHU66_000595"
     CDS             complement(7046..7873)
                     /locus_tag="ACHU66_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFR3388902.1"
                     /translation="MKSWVENVTIKKYEDRSGLTGLKQGPLVWTEVFLREVNRQSNTG
                     ILHFWPMDQTVILGMMDSQVAQLDKGLASIGQAGYSPIIRSLGGLAVVADDGILNVTL
                     IFPNPSGHKVDLRESYQVMVDLIAQALSDFPFEVVSGEVATSYCPGTYDLSIKGRKFA
                     GLAQRIYQEAIAISAYISVSGNQVKRGQVVADFYAASFAPQEVSDRFPQVNPNSMANL
                     SDLVAQDLTMEDMKIRIEHVLIENGAFLTTFYPSSDNMADFMALGKTIKQSMEKYGI"
     gene            complement(8149..9285)
                     /gene="dnaJ"
                     /locus_tag="ACHU66_000596"
     CDS             complement(8149..9285)
                     /gene="dnaJ"
                     /locus_tag="ACHU66_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR3388903.1"
                     /translation="MNNTEFYDRLGVSKNASPDEIKKAYRKLSKKYHPDINKDPGAED
                     KYKEVQEAYETLSDPQKRSAYDQYGPAGANGGFGGGAGGFGGFDGAGFGGFEDIFSSF
                     FGGGGATRNPNAPRQGDDLQYAVNLKFEEAIFGVEREVSYNREATCRTCTGSGAKPGT
                     SPVTCGRCHGSGVINVDTQTPLGTMRRQMTCDVCHGRGKQIKDPCATCHGTGHEKQAH
                     TVTVKVPAGVETGQRIRLAGQGEAGFNGGPYGDLYVVIQVQPSDKFEREGTTIHYKLN
                     LNFVQAALGDTVHVPTVHGDVDMVIPEGTQTGKTFRLKGKGAPSVRGGAIGDQYVTVN
                     IVTPTGLNDRQKAALKEFAAAGNIDVKPHKKGFFDKVKDAFEDL"
     gene            complement(9819..11642)
                     /gene="dnaK"
                     /locus_tag="ACHU66_000597"
     CDS             complement(9819..11642)
                     /gene="dnaK"
                     /locus_tag="ACHU66_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR3388904.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTIISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     EYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDHMVAEFKKEN
                     GIDLSVDKMALQRLKDAAEKAKKDLSGVTSTQISLPFITAGAAGPLHLEMTLTRAKFD
                     ELTYDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRND
                     VDQAIFATEKTLKETEGKGFDAERDQAQAALDELKAAQEANNLDDMKAKLENLNEKAQ
                     ALAVKLYEQAAAAQQAAAGQEGAQTANNAGDDVVDGEFTEK"
     gene            complement(11731..12252)
                     /gene="grpE"
                     /locus_tag="ACHU66_000598"
     CDS             complement(11731..12252)
                     /gene="grpE"
                     /locus_tag="ACHU66_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR3388905.1"
                     /translation="MNNLSEEIKNEEIVEEVEATEEVVETPEKSELDLANERAEEFEN
                     KYLRAHAEMQNIQRRANEERQTIQRYRSQDLAKKILPSLDNLERALQVEGLTEDVKKG
                     LEMVQESLIQALKEEGVEEVATDVFDPNLHMAIQTVPATDDCPAEHIAQVFQKGYKLH
                     ERLLRPAMVVVSE"
     gene            complement(12380..13414)
                     /gene="hrcA"
                     /locus_tag="ACHU66_000599"
     CDS             complement(12380..13414)
                     /gene="hrcA"
                     /locus_tag="ACHU66_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFR3388906.1"
                     /translation="MITQRQNDILNLIVELFTRHHEPVGSKALQEMIASSSATIRNDM
                     AKLEQLGLLEKAHTSSGRMPSRAGFQYFVNHSLNLEHIDEEDVYQVVKAFDFEAFKLE
                     DILERASTVLADLTGYTSVILDVEPTSQQLTSFDIVQLSSHDALAVLTLDQSKPVTVQ
                     FAIPKNFLTRDLEVLKRLVDERFVDQTVLSIHYKLRTEIPQVVQRYFATTDNVLDLMD
                     YIFSNLFQESVFISGKVASLTYGNLATYQLLDSAQLLAPELRQGLAPDQQTSISVAEH
                     SDSALADVTVIHHRFPIPYRGMGQMSLLGPVDMNYRRQMSLINIISRVLFMKLTDYYR
                     YLSSNHYEVN"
     gene            complement(13636..14331)
                     /locus_tag="ACHU66_000600"
     CDS             complement(13636..14331)
                     /locus_tag="ACHU66_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFR3388907.1"
                     /translation="MADVRLYISRHGKTMFNTIGRVQGWCDTPLTKAGEEGIRELGLG
                     LKDAGLEFKLAVSSDLGRTVQTMTIAQRELGILGKIPYYQDKRIREWCFGSFEGMYDA
                     ELFQGVLPRLKGTVDATGMTFAEIAAGIQEADTAGWAESWEVLSNRILTGFEAIAQDL
                     KKQGGGNALVVSHGMTIATLAHLLEPERGANIFLDNGSITVLKYENGKLLIEAVGDLS
                     YRKRGAELIAQGK"
     gene            complement(14407..15744)
                     /locus_tag="ACHU66_000601"
     CDS             complement(14407..15744)
                     /locus_tag="ACHU66_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFR3388908.1"
                     /translation="MDIRQVRETIEMIEEQNFDIRTITMGISLLDCIDADIEKAAEKV
                     YTKIVTKAKNLVAVGDEIAAELGIPVVNKRVSVTPIALIGAATDATDYLPLAHALDKA
                     AHEIGIDFIGGFSALVQKGYQKGDEILINSIPQALAQTTKVCSSVNIGSTKTGINMTA
                     VRDMGRIIKETAEASDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVVINVGVSGPGV
                     VKRALEKVRGESFDVVAETVKKTAFKITRIGQLVGNMASERLGVKFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDAVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPETTPAETIAAMIADEAAIGVINQKT
                     TAVRIIPLGKEGDMIEFGGLLGTAPVMKVNQASSADFINRGGQIPAPIHSFKN"
     gene            complement(15756..16022)
                     /locus_tag="ACHU66_000602"
     CDS             complement(15756..16022)
                     /locus_tag="ACHU66_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFR3388909.1"
                     /translation="MKAIVTVVGKDKSGIVAGVATKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKKDFTKLRAELEAFGQGLNVKINIQSAAIFDAMHNL"
     gene            16279..18021
                     /locus_tag="ACHU66_000603"
     CDS             16279..18021
                     /locus_tag="ACHU66_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFR3388910.1"
                     /translation="MKIIKQLWWFFSLEKKAYLIGILSLCLVSLLNLLPASIMGQLID
                     QIASGQLTEHRLLLGVAGLILSALAMYGLRYLWRMNILATSYRLGKIMRARLFNHFMH
                     LSPSFFQQYRTGDLMAHATNDINALTRLAGGGVMSFVDATVTALVTLVTMSVSISWQM
                     TLVAILPLPFMTLTTNFLGRRTHENFKASQAAFSELNNKVQEAVSGIKVTKSFGYQEQ
                     ETAAFQEVNQAAFLQNIKTMRYDALFNPAVLFFIGLSYLLTLLVGSHFISQGQVSLGQ
                     LVTFMTYLDLLVWPLMAIGFLVNISQRGDVSYNRIQTLLSISSEVIETDQPLPAPSNG
                     EIVYEISEFAYDNIPVLQDIQFHIKKGQTIGLVGPTGSGKTSLLKLLMREYDVTNGQI
                     LLNQQNIKHYKLGDLRQLMGYVPQDQFLFATSVAENIRFGNPDLSLDQVKEAAKAVHV
                     YEDIQDMPDRFDTMVGEKGISLSGGQKQRLAMARAMILNPDILLLDDSLSAVDAKTEH
                     AILETIKQERLDKTTIITAHRLSAIVHADLILVLEDGKIVERGRHQELLDEKGWYYDT
                     YMMQQLEKEESDAL"
     gene            18011..19759
                     /locus_tag="ACHU66_000604"
     CDS             18011..19759
                     /locus_tag="ACHU66_000604"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3388911.1"
                     /translation="MLSKTKQASVFWRLLSYLKAYRLLTVLALIFLLLTTVVRSLIPL
                     LASYFIDHYISSISETAAAILAAYFALYLLQMVLQYFGNLWFAKVSYSIVRDIRRDAF
                     GRMEKLGMAYFDQTPSGSIVSRITNDTESISEMFSGILSSFISALFIIITTLSTMFAL
                     DWKLTCLIVLFLPLIFLLVDQYRKRSAPIVAKTRSLLSAINSKLAESIEGIRIIQAFS
                     QEERLKEEFEAINQEHLDYASRSMALDSLFLRPALSLVKILAYALLMTYFGLDWSQAG
                     ISAGLIYAFIQYVNRLFDPLLDVTQHFSTLQTSMVSAGRVFTLMDQTFEEPLQLDRGA
                     KIDKGHIVFEDVCFSYDGKRQILDKVSFEVRQGQTIAFVGATGSGKSSIINVFMRFYE
                     FQSGRILIDGQDIRSFSQEELRSAIGLVLQDPFLFHGTIASNIQMYQDISREEIEEAA
                     RFVDAAPFIEKLPEKYDHLVTERGTAFSTGQRQLLAFARTMASQPKILILDEATANID
                     SETEQTVQTSLEKMRKGRTTIAIAHRLSTIQDADCIYVLDKGKIIESGNHEELLALKG
                     HYHRMYQLQAGQLTAT"
     gene            complement(19854..20951)
                     /gene="fni"
                     /locus_tag="ACHU66_000605"
     CDS             complement(19854..20951)
                     /gene="fni"
                     /locus_tag="ACHU66_000605"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR3388912.1"
                     /translation="MFYLGEFGLDRKDQHVGLANQQYSATPAKDFTETLFVHHSLPQT
                     KVDEVDISTSVAGLDFAFPFFINAMTGGSKKTREINRLLGIMGHFGKIALASGSVSAA
                     IKDPSVAETFSVMRRENPYGIIFANLGAHHSVENAKRAVDLLEANAIQIHVNAPQEIV
                     MPEGDRDFTMWLKNIETLVREMEVPVIVKEVGFGMSRETVAQLASVGVQTIDVSGTGG
                     TDFAKIENARRTFNDYTYLEGWGQSTVTSLVEAMSVSEEVRPSLIASGGIKTPLDIVK
                     SLALGADLVGMSNHFLQYVKDGKGHRFDDGLQAIKTYQWQMAEIMTMLGAKNIAELRQ
                     KDLVLAPNIQNWCEARGIDWKAYARRSANVS"
     gene            complement(20962..22041)
                     /locus_tag="ACHU66_000606"
     CDS             complement(20962..22041)
                     /locus_tag="ACHU66_000606"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFR3388913.1"
                     /translation="MKVQVKIPGKLFLAGEYAVVEAGYPAVIAAVDQYLTVTIETSDR
                     GLLHSSQQVDLYLIWERKEGIAHVQGDHPYALIETVMQVTEAYLTAKGYACHGTYSLA
                     VQSDLDDQTSGAKYGLGSSGAVTVATVKALLTYYGHRPDALLTYKLAALAQTKLGMTG
                     SFGDLAASSFGGLIAYRSLDRSWLLGKMAELSLLDLVESDWQSLSISPIQLPQGLDLL
                     VGWTGSAASTDSLVSQMESQKSQIEKEQIHVQFLADSKICVEQLIVACQTNDSASVRQ
                     ALTQNRKLLQDFARGMGLVIETPQLSQLCDLAQTYGAVAKSSGAGGGDCGICLVDSKE
                     QKAAIETAWQEAGIFPLTLNIASRE"
     gene            complement(22043..23068)
                     /gene="mvaD"
                     /locus_tag="ACHU66_000607"
     CDS             complement(22043..23068)
                     /gene="mvaD"
                     /locus_tag="ACHU66_000607"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFR3388914.1"
                     /translation="MTKQTGIARAHTNIALIKYWGKRDKELFLPMNSSLSLTLDAFYT
                     DTKVVFDPELTADEFYLNGMLQKEKEILKISRFLDLFCEYIGERAFARVESLNFVPTA
                     AGLASSASAFAALALATATALDLDLSPATLSTLARRGSGSSTRSLFGGFVEWDMGTGS
                     EDSMAHPIDDADWDIGMVVLAVNTGPKKIASREGMDHTVATSPFYTAWVETAKQDLVD
                     IKAAIASRDFEKLGQITEHNGMKMHATTLSANPPFTYWSADSLVAQEAVRQVREATGL
                     SAYMTMDAGPNVKVLCRTSQMDELVAELAKVFPREKIITSKPGPAAYVLSEDEWQTSQ
                     AAFEKGL"
     gene            complement(23061..23996)
                     /gene="mvk"
                     /locus_tag="ACHU66_000608"
     CDS             complement(23061..23996)
                     /gene="mvk"
                     /locus_tag="ACHU66_000608"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFR3388915.1"
                     /translation="MSVVGKANGKIILMGEHAVVYGQPAIAMPFSAVEIIAQVTAQGE
                     ALTVACDFYKGLVHKMPKIWESLKHAIRFSLYRIGAPTDPAIHIEISSTIPAERGMGS
                     SAAVAVAVARALFAYYKKELTDSELWDIVQSSEKIAHGNPSGIDAATTSGKSPVFFIK
                     HQPIEPFELKFHAHLVVADTGVTGNTLEAISDVADLLEKKPEAIKLVEELGNLTRQAK
                     EDLATDQAELLGSRMNQAHALLQKLGVSDPSLDKLVSLAQENGALGAKLTGGGRGGCM
                     IALARTAQDAQKLAHILDQAGARQTWIQYLGETND"
     gene            complement(24351..25628)
                     /locus_tag="ACHU66_000609"
     CDS             complement(24351..25628)
                     /locus_tag="ACHU66_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFR3388916.1"
                     /translation="MNKQTYKEILTIAFPAMGENLLQMLMGVVDSYLVASIGIVALSG
                     VSLANNILAVYQAVFIALAAAVSSRLAQALGEGGSEGIGGTASESVKFTVFIGLLLGI
                     FSVLAGPIVLASLGAESEVVQAGGLYLILVGGGAPFWGLMASLSAILRTLGEPRFPMY
                     ISLLSNVLNALFSAFAVFVLHAGVAGVAIGTVLSRLIGCSLLWSRLPISLKPWTWSFD
                     KELLRLALPATGERLMMRVGDVVVVALITSLGTATVAGNAIGETLTQFNYMPALGIAT
                     ATIILTAKHRQDKAMVRHIFRTSFGLSLLFMFLVAGVTYFAGPFLISLYTKDIQAVQA
                     SQTVLFYAMLGVPFTAGTLIMTALWQGLGNARLPFYATTIGMWLVRIGIAYLLITFFK
                     IGLSAIWIATILDNAFRAGVLFFQYRYSKQESI"
     gene            complement(25634..26347)
                     /locus_tag="ACHU66_000610"
     CDS             complement(25634..26347)
                     /locus_tag="ACHU66_000610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR3388917.1"
                     /translation="MKKLQVKDLMVTGAFAALYFVCVGLGTLLSLVFDRSGNMMYAPA
                     GAALLAGPVYMLLIAKVGKPGSISLVGANGSGKTSLARYLVGLAEDKKSRISWQGQTL
                     SSRQRLEKTAFVMQDVRLQLFAESVERELTLGRKNRAVDEHLVARFGLSDLLERHPVS
                     LSGGEQQRVMIVASLLADKEIFIFDEPTSGLDLRRMQQVASALVDLKMKNKLVLLISH
                     DEELLNLVCDKIVDIKQLK"
     gene            complement(26348..28009)
                     /locus_tag="ACHU66_000611"
     CDS             complement(26348..28009)
                     /locus_tag="ACHU66_000611"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3388918.1"
                     /translation="MDKKEMLTRVLIPRLLQSMKHLLPRIAVAVCFAVLGQVVTIAIP
                     VTLVYLAFAALAGNPAPLWTLGILILLALLRGAFRYGEHYFGHYVAFHTLAAYRRLIF
                     AKLRALAPGKLDRQDSGSLLKMIGEDIEALEVFFAHTIAPICTGILVAFGLTVYFGLS
                     SWGLALLALVTYALLAIAIPNRFAQQLEPLLKEQNASRKGYVSYFIESLKSMKDLMQF
                     QQTDARFAKLTQKSQEVNGQERKVAQTNFMQYAVSFLVVGLSIMCFAWLTFDLVGSQS
                     LDLATGVALLVSFTSSFAPFLELSRLPLGFKRAMNAGRNIYALLDEKEAERTGELVEV
                     RVTDIAIEQLDFDYDDRETGLYRNLSVQFEQGGIIGLVGESGAGKSTLMKLIMRWYDW
                     QQGQIRLSGLDSRQVDKAHLQGAFAYVPQVPQIFRQTIRENLLLGRTDVSDETIMDLA
                     EKCHMKERILAAPQGLDTLVEASDFSAGEGQRLELMRALLKNADCYIFDEPTSNLDSL
                     NEARFIQLVKEHCQGMVFLISHRSSTMACADTIFYLEDGQLVKEK"
     gene            complement(28002..29718)
                     /locus_tag="ACHU66_000612"
                     /pseudo
     CDS             complement(28002..29718)
                     /locus_tag="ACHU66_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983224.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
BASE COUNT         7960 a         7361 c         6354 g         8043 t
ORIGIN      
        1 tgttataatt aacttagtta aatgataata attctaaata aggaagagca aactatgaca
       61 ctcgtaacca acctcaaaca acacagcaac atcatcacga ccgctctctg cttggcgctc
      121 attctaatag gtatccttct gtttcagact ggacagggat gggctcctat cctcttcatc
      181 tcagcctttg tcatcggggg ctaccagtca gccaaggaag gcatttctga gctgattttc
      241 gacaagcacc tgtctgtcga cctgctcatg attttagctg ctatcggctc tggtctaatt
      301 ggctactgga tggaaggggc tctactcatc ttcatcttct ccctttcttc tactctggaa
      361 gaattagcca tggaaaaaag caagaatgct attgcagctc tgatgaacat gacgcctcca
      421 acggctcgca aaatcgagga aaatggtgac attactgtct tagacaccgc agccatccgc
      481 atcggcgacc tcctgcaagt ccgcaagggt gacacggtcc cactggatgc taccctcatc
      541 agtccgcaat ccatctttga cgaatccatg attacaggcg agccgctccc tgcggagaaa
      601 atgactggcg ctgctgttat cggcggaacc attaaccaag gtccaactgt gaccgtacaa
      661 gttacggctg aaaaaggcga tgccctcttc gataaaatcg tccaaatggt tgaaaacgcc
      721 caagaatcca aatcaaaaac agcgactttc attgaaaata tggaagatac ctatgtcaag
      781 attgtcttgg tggtggtacc actcttcatt ctctttgctc attttgctct gtgctgggac
      841 tggttaactg ccttctaccg tggcatgatt ctcttgacca tcgcttctcc atgcgccctc
      901 gtagcttctt cttcgccggc gacactttct gctatcagcc gtgcggcacg caagggtatg
      961 attatcaagg gtggtgatat tgcggataac atcgccaatc tagaagccat cgtctttgac
     1021 aagacgggca ccctgaccat tggtaaacct gaagttgtag acgcgaccta tcttggcgat
     1081 gaaaaactaa tcagggaagt tgttcaggca gttgaaaaac aatcaagcca cccaatcgct
     1141 caggctctca tgacctatac ggctgacagc tctgctattg ccctccaaag cctagaagac
     1201 gtgaccggaa aaggcttggt ggctggctat gagggcgaca gctggaaaat aggtaaggca
     1261 ggatttgtgg tggacagcct ggtgagtccc ctgtctgctg gcttgctggc acagatcgat
     1321 gaggcggaaa gtactgggaa aaccctggtc tatgtcagcc aaaatgatgt gctggtggca
     1381 atcttcatgg tggaagatag tttgaaaccc gagagcaagc agctaattgc ccagttgaaa
     1441 gagatgggcg tgacgccaat tctcttgacg ggtgaccaag aaaagacggc tcgttacgtg
     1501 gcgagtcagg tcggtattga ccgtgtgatt gccaactgtc tgccgacgga taaggctgct
     1561 gttatccaaa aactgcaaac cgagtttgcg tcagtcggta tggtgggaga cggtatcaac
     1621 gatgctcctg cccttgctca agccaatgtc agctatgcta tgggaagcgg tacggacatc
     1681 gctatggagt cagctgacat cgtactcatg gaagacctga cgagaattcc ttactccatt
     1741 cgtctctcta agaaaatgcg gggcatcatc aagcagaaca tcatctttgc tctgtccgtc
     1801 attgctcttc ttatcgtttc caacctcttc cagtctatca acctcccact cggtgtggtg
     1861 ggccacgaag gatcaacgat tttggtgatt ttgaatgggt tgagattact gtattttaaa
     1921 tagcttcaaa ccgccagcta cttggcggtt ttcttattat tcttttttta aaaaaacgct
     1981 tgcattctaa aaattattgt tttataataa ttttacagat aatattatca taaaacaata
     2041 ataatcggag gtttgctatg aaaaccaatc tactcaaaac agcaggattg ttagctggtt
     2101 gtgttcgcta tttcttctat ttattaattg caggctatat tttctttagc ttggctggtt
     2161 tccgaggcga ctcatttgtg ataggctctt gggaatacac ctttaacaaa cccattgacc
     2221 agattagtgt ttggctcatc cttttaacaa gtggattaat cttaatcatc ttggcggtac
     2281 tcagtcacat tgcccacttg attcaaaagt tatgtaaatt gcttctggaa gaagattatt
     2341 tcgcttcagc tagtctggaa ctttatcaga aactcttcat cagtttgctc gtcttgactg
     2401 ctgggcaatt ttgcctaacc agcttgatca ccttgacaaa tgctgctgga ccaaacaact
     2461 tcctcaatct gcgttggtca gatttcatcc tcaatgctct ctttatcttc ctgacttatt
     2521 ttgtctggac cttagtacaa aaagggcaac aacttgaaac cgagaacagc gagtttatct
     2581 gatatggaaa tgattcgcgt aaacctagac aaggtcctca aagaccgaca aatgacttcc
     2641 aaggacttgg cggagcaagt cggcatcacc gaagccaatc tgtccattct caagacaggc
     2701 aaggcaaaag ggattcgttt caataccctg atgagcatct gccgcatcct agactgccaa
     2761 ccaggagata ttttggaata tgtgaaggag ggggaatagc atgaacaaaa agatattttt
     2821 caacatcgtc atacagttgc taacgtttat tcgtcatgga ttgattgcct taacgggact
     2881 gataatgttg gcacttctta ttctacttat aggcgggaaa cctattcaaa atagtctaca
     2941 attagacgtc cccttgccta tcattgttat tattctaatc ataagtatcc tattggtagc
     3001 ctgctttatc tacatggcca ccatactcaa aaaattgctc atcaactttc aaacaggaac
     3061 tattttctca actgaaaatg tagtctatac aaagataatt ttaggaacct taatcatatg
     3121 gacgagcata caatttgcaa gtgctttttt cctatcttac ctagatttag agaatgtaag
     3181 tgatttatat gatttttcac ttagtgacta cttcataaat ggtgtgctga ttattattgc
     3241 actactagca aagatggtgt tggaaaaagg tgtgctttta caagctgaaa atgatgaaat
     3301 tatataaagg agcccatgat gaaaaagtat atttccctca ttgcactctg catgatgata
     3361 ccttgcttaa gtgcttgtca ctctgtcact tcagaagtta ataaagaaat gcaaaacgag
     3421 ctaacagcag cttccattga aacagaaaaa gctctaaaaa cagatgaacg tctggctgag
     3481 attgagagct ttcagcaaat tgtcgaaatc gctgcatcca aaggagagtt ctctcttgaa
     3541 tcttctttct ctggcaaggt ctatttaatg gcaacaaaag aaaatctcga gaaactctca
     3601 aaagaagagt ttactctgaa taaaaagagc ttctcaacta tcgatgctgg tagtagcaat
     3661 gattttaata tccgctacaa acatcaaact tacttcactg ttcaaagcct atccgtgcaa
     3721 gatatggaag attttgaata caccaaatcc tcttccactt ccgctattct tgtgttctat
     3781 ccaaactaga aaaaattcct cgattcaaaa gaaccgagga attttttaat aagtcgccag
     3841 gcgtttttct aacctgagct gaccaaaggc gtagagagtg cagatggccc aatatatcaa
     3901 ggcaacacag atataaactg tcatgtaatc agacttggct ccgccgacta tcttggcctt
     3961 attgaaaatc tctggtaccg taatcatggc tgtcagagag gtacttttga ccatgtctaa
     4021 gaggacattg ctcagcggtg gaagggcaat gcgaaaggct tggggaataa tgatttttct
     4081 gtaaatcaca ttggtccgca agcccaggga acgcgctgcc tcccattgtc ccttatcaat
     4141 ggccgaaaga gatgcacgaa taatttcaga aatgtaggca ctggacatgg aggtaaaggc
     4201 gataatagat gcggagatag catctagttg tagccccatg aaaggcaatc caaaatagat
     4261 gaaaaagaga aggaccatga ggggaattcc acgcatgaga gaaatatggg ccatagccaa
     4321 ccaacgcaag gggccaaact tagacatccg catgaaggct acaaaaaatc cacagaaagt
     4381 ccccaaggca aaaccaatta gggataggga cagagtgtag ggaagtccct ccaaaatctg
     4441 tggaatagcc tctcttgcaa tatctgcatt aaaaactgcc tgccagttaa tatccatatc
     4501 ttctcctctt tcctaaaaac cagatagacc ttgccagcct gtgactgaca aagtctatac
     4561 aagatgcatt taatttgtta cgaggtctta ttcaacttcg ataactggca attggtctgc
     4621 attttcaaca gggactgtca aatcttgtcc cgcatagtat ttctcagaaa ttgccttcaa
     4681 agttccgtca gctttcatcg cttcaagagc ttcgtcaatt ttttctttca agcttgtgtc
     4741 agccttgctc atgacaatac cttgttcagt tggattgtat tttgcatcac ccatcttcac
     4801 tttgatatct gggaagttag catttgccca tttgatggcc atgacttgtg tatagtagtc
     4861 gtttggaatg aagtctgtac gacctgttga tacatcacgc aaatagacat cattggttac
     4921 gttgtcatag ataactggct ctgctccttg tttttcagca attttcatgt actgagtacc
     4981 tgcgccacca ccagcttttt taccagtcca gtcgctcaag tcagctgctt cgattcctga
     5041 tgaaccatct tcacgaacga tgtagccacc aactgaatac ttgtaaggga tagagaagtt
     5101 atacttttcc ttacgtgctt ctgtcatgtc aaagttgtta acagaaatat cgaccttacc
     5161 actgtccaca gctgtaaagg cttctgccac accgatttct tgatattcga tttccaattt
     5221 caaacgttta ccaatttcat tgatcatgtc aatttcatat ccgaccaatt ctttagcatc
     5281 gttgtagtaa gaagttggga aaagggtccc tggtgttgca accttcaaga cacctgcttc
     5341 ttgtactttt tcccattgag tctgttcagc agaagaactg ctagtattag aacttgagct
     5401 agaacatgct gccaaggtca aagtagaaac caagagagct gaggccaaaa cttttttcaa
     5461 cataaaacaa tctcctttgg aagctatagc ttcctttttt actttttcgg tgttaaccca
     5521 ccaactttta caggttaaca ttttgaaaac gttttggcaa ataattgaac tgaaaaaatg
     5581 aattccctta caagttgagt aaacctgccg cctccatttg atctgccaac cgcaagagta
     5641 aatcctctcg tcccttggat gctgttaatt ggatgccgat tggtaagccg tcagcccgca
     5701 cataagtagg gagagaaatg gctggctgac cacaaatatt ggcctgctgc gtaaagggag
     5761 ttagggctag gctgtcctcg aacatttgcc agatcaagtc ttgctgctct tccatagaat
     5821 agttctgggt ctgcttgagg cggtccagca aatctgctga taggtcaaat tgtccatgct
     5881 tgggcgctac atcagccact gtcggcgtca ggagcaagtc gtattcctca tggaaacgcg
     5941 ccatgctggc tccaaactga tcccagtcca atagggcctt ggagtgcaat ttggctggaa
     6001 tggtttgccc gctttggtaa atagcccagg acatgacttc catgtcatcc agggtcatct
     6061 ggcgcccaaa agccgcttcg atttcgtcaa acatctgggc cgtgtccacg ctgttcatca
     6121 ggtagaagga tttcatcagg gccactccat ccagaggata ttctgataat tccacaactt
     6181 catgccccaa atttgccaag tgatgacagg ctttttgaac cgcttctacc gcttcaggac
     6241 tgaccttgct ccctactgga gatttggtat agtaggcgat ttttaggggg cgagacaact
     6301 ttccaaacac ctcttcttcg ctcagcttgg caaggggaaa gggggcttcc agctggtagt
     6361 cctgaaaatg atagagcaga cgctgggtgt cacggacaga cttggtcagg gcaaaattga
     6421 cagatgctcc ctgccagcca cgataagact taggtccaac tggaatccga ccacgactgg
     6481 ttttgagccc gatcaagcca ttgaaggagg ctgggatgcg aatagaccca ccaccatccg
     6541 atgccgcagc aatagagacc atgcctgaag ccagtgctgc agctgcacca ccacttgatc
     6601 caccagcatt gcggctagga tctaagggaa gcttgacagg accgtgcagg ctggcatctg
     6661 agatattttt gaagccgaat tctggcgtgt tggttcgacc gacaatgatg aaacccaagt
     6721 ccttcaagcg ttggacatag gtatcggtat ggcctgctgg tcgaccagca agcaggcgag
     6781 aaccagcaga agatggctgc cccttttcat tttgccccag atccttcaac aagaaaggaa
     6841 ctccagcaaa tggcttgcca aggtagtctt ctttttcagc ttctttcaag gcctcttcat
     6901 actgcttgct gacaaccgca ttgagggtgg gattgagctt ttcaatcctg tcaatggttt
     6961 cttgcaccaa ttccttggca gaaacctgtt tttgattgac agcctgagcc atggctgtcg
     7021 catctttcca tgtcataaga atctcctaga taccatattt ttccatagac tgcttgatgg
     7081 tctttccgag cgccataaaa tcagccatgt tgtccgacga aggataaaag gtggtcagaa
     7141 aagccccatt ttcaatcaac acatgctcga ttcgtatttt catatcctcc atcgtcaaat
     7201 cctgagcgac caagtctgac agattggcca tcgaattcgg atttacttgc ggaaatctgt
     7261 ccgatacttc ttgtggagca aaacttgccg cataaaaatc agccaccact tgcccacgtt
     7321 tgacttgatt gcctgataca ctgatatagg cagaaatggc aatagcctct tggtaaatcc
     7381 gctgagccaa tccagcaaac ttccgcccct taatactaag gtcgtaggtt ccagggcagt
     7441 aggaagttgc cacctcacca ctgaccacct cgaagggaaa atctgaaagc gcctgggcaa
     7501 tcaaatccac catgacctga taagactccc gcaaatcaac cttgtgacca gaaggattgg
     7561 gaaaaatcag agtgacattt aaaatcccgt catccgcaac gactgccaaa ccgcccaaac
     7621 tccgaataat aggactgtag cctgcctgac caatactggc taagcccttg tccagctggg
     7681 caacctgact gtccatcatc cccagaatga ccgtttggtc catgggccaa aaatgtaaaa
     7741 tccctgtatt ggactgacga ttgacctcgc gcaaaaagac ctcagtccac accagtggcc
     7801 cctgtttcaa acccgtcaaa ccagaccgat cttcgtattt ctttatagtc acattctcaa
     7861 cccaagattt cattcgtaag cgttctcttt caaattacta tatcttattt taacacagag
     7921 cctgtgatgc gtcggaaaaa atgcccagag gattttctga aaaagaaagg ggagtgggaa
     7981 agaactcgac caagataaaa agagttcgtc ttcccacccc cgcacagttg attaggccag
     8041 atttggagtg caaaacacga acaaatctgc caatcaacca ctgcgctgag atgttgaaac
     8101 gaactctgag tagtggtcct gggctttttg cccagtctca tcttagaatt ataaatcctc
     8161 aaaggcatct tttaccttgt caaaaaatcc ttttttatgg ggtttgacat caatatttcc
     8221 agctgccgca aattctttaa gagctgcttt ttggcggtca ttcaaacctg tcggagtgac
     8281 gatatttact gtgacatatt gatcaccaat agctccacca cgaacacttg gtgcaccttt
     8341 tcctttgagt cggaaggtct taccagtctg agtaccttca ggaatgacca tatccacatc
     8401 accgtgaact gttggcacat gaactgtatc tccaagtgct gcttggacaa agttgaggtt
     8461 cagtttatag tgaatagtgg ttccttcacg ttcaaacttg tctgaaggtt ggacctgaat
     8521 gacaacgtag agatctccgt atggacctcc attgaaacca gcttcacctt gacctgccaa
     8581 gcgaatacgt tgaccagtct caacaccagc tggcacctta accgttaccg tatgggcttg
     8641 tttttcatga ccagttccat gacaggttgc acatggatct ttaatttgtt ttccgcgacc
     8701 atgacagaca tcacaggtca tttgacgacg catggttcca agtggagttt gggtgtccac
     8761 attgatcact ccagaaccat gacaacgccc acaggttacc ggacttgttc caggtttggc
     8821 accggaacct gtacaggttc gacaagttgc ctcacggtta taggaaactt cacgttccac
     8881 accaaaaatc gcttcttcaa atttcaaatt gaccgcatat tgcaagtcat ccccttgacg
     8941 aggtgcatta ggattgcgcg tcgcaccacc tccaccaaag aagctagaga aaatgtcttc
     9001 aaagccaccg aaacctgctc cgtcgaaacc accaaagcca cctgctcctc cgccgaagcc
     9061 tccattggca ccagcaggac catactggtc ataggcagaa cgtttttgag ggtcgctcag
     9121 agtctcataa gcctcctgga cctccttgta tttatcctcc gcacccggat ccttattaat
     9181 atctggatgg tattttttcg aaagcttccg ataagccttc ttaatctcgt ctggcgaagc
     9241 attcttagaa acccccagac gatcgtaaaa ttcagtattg ttcatataag atactaaggg
     9301 cgtaaagcgg acacagccaa aataggagtt ttgacgcagg acctcaccgt cctaggaaaa
     9361 actatctttt tggcacagtc cgtagcccgt gttcgattac gaacactttc ctttctatca
     9421 tagttttgga aaagtgaaaa taggaaactg acgcagtgtg taaaacacac taggaggttt
     9481 atctttttca ctaggttttt agcctgtgtt cgaataaacg aacttgtatt cctttctttg
     9541 aaaattagaa agtagcccgt gttcagttac aaacactttc ctttctgtaa aagttagaag
     9601 ggtatttacc caaaaacaga ggctgggttg caacctctgt gattggaaat tttattgacg
     9661 aatgatttct attctcagga agataagaat tgagttcgag ctaagaacct taaccgactt
     9721 cctactaatt ttcaagaagt caggataaaa aggtctccca gtccaaccgc tcgtttctca
     9781 tttctggctc acgggctaaa atagtccaca ggactatttt acttttccgt aaactcgccg
     9841 tctacaacat catcgcctgc gttgttagct gtttgggcac cttcttggcc tgcggctgct
     9901 tgttgtgctg ctgcggcttg ctcgtagagt ttcactgcaa gggcttgggc tttttcgttg
     9961 aggttttcaa gtttagcctt catgtcgtcc aagttgttag cttcttgggc tgctttcaac
    10021 tcatcaagag cagcttgggc ttggtcacgt tctgcgtcga aaccttttcc ttcagtttct
    10081 ttaagagttt tctcagtcgc aaagatggct tggtcaacat cgttacgaag gtccacttct
    10141 tccttacgtt tcttatctgc ttcagcgttt gcttctgcat ctttcatcat gcggtcgatt
    10201 tcttcatctg tcaaacctga gtttgattgg ataacgatag tttgctcttt ttgcgtacca
    10261 aggtctttag cttttacaga aacgataccg ttcttgtcga tgtcgaatgt tacttcgatt
    10321 tgtggaatac cacgaggtgc tgcagggatg tcagtcaatt ggaagcgacc aagagtcttg
    10381 ttgtctgcgg ccattggacg ctcaccttga agcacgtgga tatcaacagc tggctggttg
    10441 tctgccgcag ttgagaagac ttgtgattta gaagttggga tagttgtgtt acggtcaatg
    10501 agttttgtaa agacgccacc cattgtttcg ataccaagtg acaatggagt tacgtcaagg
    10561 aggacaacgt ctttgacatc accagtgatg acaccacctt ggattgctgc acccatggca
    10621 accacttcgt cagggttaac agatttgttt ggctctttac cagtttcagc cttaacagct
    10681 tccacaacgg ctgggatacg agttgaacca ccgacaagga taacttcgtc gatttctgac
    10741 aagctaagtc ctgcatctga aagagcttga cgaacaggta cttttgtacg ttctacaagg
    10801 tcataagtca attcgtcaaa ttttgcacga gtcaatgtca tttccaagtg aagcgggcct
    10861 gccgcaccgg cagtgataaa cggcaagctg atttgagttg atgtcacgcc tgacaagtct
    10921 ttcttagctt tttcagccgc atctttcaaa cgttgaaggg ccatcttgtc aactgacaag
    10981 tcgataccgt tttctttctt gaattctgct accatgtggt cgataatctt ttggtcaaag
    11041 tcgtcaccac cgagcttgtt gtcacctgct gttgcaagta cgtcaaagac accgtcacca
    11101 agttcaagga tagatacgtc gaaagtacca ccaccaaggt cgaataccaa gattttctcg
    11161 tctttgtcag tcttgtccaa accgtaagca agagctgctg cagttggttc attgacgata
    11221 cgttctactt caagaccagc gattttacca gcgtctttgg ttgcttgacg ttgtgcatcg
    11281 ttgaagtaag caggaacagt aataacggcc ttggttactt tttcaccaag gtattcttca
    11341 gcgtagcctt tcaaatattg aagaatcatt gctgagattt cttgtggtgt gtattctttg
    11401 ccatttgctg aaactttttc agaagttccc atttttgatt tgatagagat gatagtatct
    11461 gggttagtta ctgcttggcg ttttgccgca tcaccaacga tgatttcacc atttttgaaa
    11521 gacacaacag acggagttgt gcggttccct tctgggtttg cgataatttt tgattcagtt
    11581 ccttcaagaa ctgcaactgc tgagttagtt gtacctaagt caataccgat aattttagac
    11641 atgtgtgtta cctcttttta atttactttc ttttcaagta gtttaacgac atctcggtca
    11701 ggattctttt tttggggaat agcagagcgg ctactctgat acgactacca tagccggtct
    11761 cagcaagcgt tcatgcagct tgtagccttt ttggaagact tgtgcaatgt gttctgctgg
    11821 gcagtcgtct gtggctggaa ctgtttgaat ggccatgtga agatttgggt caaagacatc
    11881 ggttgcgact tcctccaccc cttcttcttt gagggcttga atcaagcttt cctgtaccat
    11941 ttccaagcct tttttgacat cctctgtcaa gccttcgact tgaagggcac gttccaagtt
    12001 atccaaactt ggcaagattt tcttggccaa gtcttgcgaa cggtagcgtt gaatagtttg
    12061 gcgttcctcg ttggcacggc gttggatatt ttgcatttca gcgtgggcac gaaggtactt
    12121 gttttcaaat tcctccgcac gctcatttgc caaatccaat tccgattttt caggtgtctc
    12181 cacaacttct tctgttgctt caacctcttc tacgatttct tcgtttttga tttcttctga
    12241 caagttgttc acctcttcat tttctattat tactttgaac aataataaat tgagttcggg
    12301 ctagaaactc agaaaagtga aatagtccag tggactgttt cagcctattt ttcaatcgtt
    12361 tccttcacgc cctcacatct taatttacct cataatgatt actgctcaaa taacggtagt
    12421 aatcggttaa tttcataaat aggacgcggc tgatgatatt gatcaaactc atctgcctgc
    12481 ggtagttcat gtcaactgga ccgagcaaac tcatctggcc catgccccga taggggattg
    12541 gaaaacgatg atgaatgacc gtcacatctg caagggccga gtcgctgtgt tctgccacgg
    12601 aaatgctggt ctgctggtcg ggagccaagc cctgccgcaa ctctggtgct aatagctgtg
    12661 cgctatccaa gagttggtag gtagctagat tgccgtaagt cagcgaggca acttttccac
    12721 tgataaagac cgattcttga aaaagattgg aaaagatgta gtccatcagg tccaagacat
    12781 tgtccgtcgt ggcaaagtaa cgctgcacca cttggggaat ctccgtccgc agcttatagt
    12841 ggatggataa gaccgtctgg tcaacgaagc gttcatccac caggcgtttg agcacttcca
    12901 agtccctggt caaaaagttt ttaggaatgg caaactggac ggtgactggt ttggactggt
    12961 ccagagtcaa gactgccaga gcatcgtggc tactgagctg cacgatgtca aaggaagtca
    13021 actgctgact ggtcggctcc acatctaaga tgaccgaagt gtagccagtc aaatccgcta
    13081 ggactgtact ggcccgctcc aagatgtcct ccagcttgaa ggcttcaaag tcgaaggcct
    13141 tgaccacctg ataaacatct tcttcatcga tgtgttccag attgagcgag tggttgacaa
    13201 agtattgaaa accagcccga ctgggcatcc gtccacttga tgtgtgggcc ttttccagca
    13261 aacccaactg ctctagcttg gccatgtcat tgcgaatggt agccgaactt gaagcaatca
    13321 tttcctgcaa ggccttagaa ccaactggct catgatggcg cgtaaataat tcaacaatca
    13381 gattcaaaat atcattttga cgttgggtaa tcatctccgc tcctttcatg aacacgattg
    13441 atttttagct ttagcactct cgtatgacga gtgctaaact atatactccc attatacgcc
    13501 gaaaaaatgg attgtcaaga gaaaaagaca aaaaattagc actcttttac ctagagtgct
    13561 aaaaatcagt ctgaggacct agttacttcc ctacttcaat caaaaaaatc agagaatatc
    13621 cctgatttca tatctttatt tcccttgggc aatcaattct gctccacgtt ttcggtagga
    13681 taggtcgcca actgcttcaa ttaaaagctt gccattttcg tatttgagga cggtaattga
    13741 gccgttatcg agaaagatat tagcaccacg ctctggctct aacagatgag ccagggtggc
    13801 aatggtcatg ccgtgactaa ccacaagagc attgccaccg ccctgttttt tcaaatcttg
    13861 ggcaatagcc tcaaaacctg tcaagatacg gttactcaag acctcccacg attctgccca
    13921 gccagcggtg tctgcttctt gaatacctgc tgcgatttcc gcaaaagtca tgcctgtcgc
    13981 atcaactgtc cctttcaaac gaggcaggac accttggaaa agctcggcat cgtacatgcc
    14041 ttcaaaacta ccgaaacacc attcacggat acgcttgtct tggtagtaag ggatttttcc
    14101 caaaatcccc aactcacgct gggcgatggt catggtctga acggttcgac ccaggtcact
    14161 ggacacggct agtttaaatt caaggcccgc atccttgagc cccaagccca attcacgaat
    14221 cccttcctca ccagccttgg tcagcggcgt atcacaccag ccctgcacgc gcccaatggt
    14281 attaaacatg gttttcccgt gacgagaaat atataatctt acatctgcca ttcgtttttc
    14341 tccttgtcat ttcttactat tagtataaca aaaaaccgac tgcttgtggc aatcggtcta
    14401 cgttttttag ttcttaaagc tgtgaatcgg tgctggaatt tgtccgccac ggttgataaa
    14461 gtccgcagaa gaagcctgat tgaccttcat gactggcgct gttcccagaa ggccgccaaa
    14521 ttcgatcatg tccccttctt ttccaagcgg aataatgcgg accgcagtcg ttttctggtt
    14581 gataacccca atggccgctt catccgcaat catagctgca atggtttcag caggtgttgt
    14641 ttctggaata gcaatcatgt ccaaaccaac agaacagata gccgtcatag cttctaattt
    14701 ttcaaggtta agggagccat tttgcaccgc agctatcatg ccctcgtcct cagacacagg
    14761 gatgaaggca cctgacaagc caccgacctg gttacaagcc atgaccccac cctttttcac
    14821 tgcgtcattg agcagagcaa gggcagcagt cgttccgtgc gtaccaacgg tttctaagcc
    14881 catttcctcc aaaactcgtg ccactgagtc gccaaccgct ggcgttggag caagggacag
    14941 gtccacgata ccgaacttga cacccaggcg ttcgctggcc atatttccta ccaactgacc
    15001 gatacgggta atcttgaagg cggtcttctt gacggtctcc gccaccacat caaagctctc
    15061 gccacgcact ttttcaaggg cacgcttgac cacaccaggt ccagacacgc cgacattaat
    15121 gaccacatcc gcctcaccga caccatggaa ggcacccgcc ataaagggat tgtcctcgac
    15181 cgcattggca aagaccacca acttggcagc ccccatatca gaagcctccg ccgtctcctt
    15241 gatgatccgc cccatgtccc gcacagccgt catattgata cccgtcttag tcgagccgat
    15301 gttgaccgac gagcagactt ttgtcgtttg ggccaatgcc tgcggaatag agttgatgag
    15361 gatttcatct cctttttggt agcccttttg aacgagagct gaaaagcccc cgataaaatc
    15421 aattccgatc tcatgagccg ccttgtccaa ggcatgagct aggggaaggt aatccgtggc
    15481 atccgtcgcc gcaccaatca aggcaatagg cgtcaccgac acccgtttgt taacaacagg
    15541 aatccccagc tctgccgcaa tctcatcgcc gactgctacc aagttcttgg ccttggtgac
    15601 aatcttggta tacactttct ccgcagcttt ttcaatatcc gcatcaatac agtccaagag
    15661 ggaaattccc atggtgatgg tccgaatatc gaaattctgc tcctcaatca tttcaatggt
    15721 ttctctaacc tgtctaatat ccatgaaatc tctccttaca agttgtgcat agcatcaaaa
    15781 atggctgcac tttgaatgtt gattttcacg ttcaaacctt gaccaaaggc ttctagttct
    15841 gcacgaagct tggtgaaatc tttcttctca tccgacgaca ccaccgccat catggtaaaa
    15901 tactcatcca aaacagtttg ggaaatatcg tcaatattga gccctaactc cgcaatctta
    15961 gtcgcaacac ccgcaacaat tcctgacttg tccttaccga caactgtaac aatagctttc
    16021 atctgaacac ctcttctaaa ttttcattta ttttatcata aatagacaga aaaaggtaaa
    16081 aatcttttga tttctacctg tatcgtacgt gtttacaact ccaactgttt cttctattcg
    16141 gctacggcta gagttgccta gtttttaagt tgcaaacttt ctatttctac cagtctgtga
    16201 tacactagtg tcaccgaatt ctattatttg ttggttgcga caagctacca acctttcttt
    16261 tgttcagaaa ggatacttat gaagattatc aagcaattgt ggtggttctt ttcccttgag
    16321 aaaaaggcct atctgattgg gattttatca ctctgtttgg tcagcctcct caatctcctg
    16381 cccgcctcta tcatggggca gttgattgac cagattgcca gcggacagtt gactgagcat
    16441 cgcttgctac tcggggtagc tgggctcatc ttgtcagcct tggccatgta cggcctccgc
    16501 tatctttggc gaatgaacat cctggcaaca tcttatcgtc taggaaaaat catgagggct
    16561 cggctgttta accactttat gcacctatcc ccttcctttt ttcagcaata tcgaacaggg
    16621 gatttaatgg cccatgcgac caatgatatc aatgccttga cacgactagc aggtggtggc
    16681 gtcatgtcct ttgttgatgc taccgttact gccttggtta cactggtcac catgagtgtg
    16741 agcatttcct ggcagatgac cttggttgca atcctccccc tgcccttcat gacactcact
    16801 actaattttc taggccgtcg tacccatgaa aatttcaagg cttcacaagc agccttttca
    16861 gagcttaaca acaaggtaca ggaagctgtt tctggtataa aggtcactaa atcctttggc
    16921 taccaagaac aagaaacggc ggcttttcag gaagtcaacc aagcagcctt cttgcagaat
    16981 atcaaaacca tgcgttacga tgccctgttc aaccctgccg tcctcttttt tattggcctg
    17041 tcctacctgc tgacccttct ggtcggctct cacttcatct cgcaaggtca ggtcagtctg
    17101 ggccagctag taactttcat gacctattta gatctcctcg tctggccgct catggccatc
    17161 ggttttttgg tcaatattag ccagcgtggt gatgtatctt acaatcggat tcagaccctt
    17221 ctcagcattt cctcagaggt cattgaaaca gaccaaccgc tgcctgcacc aagcaatgga
    17281 gagattgtct atgagattag cgaatttgct tatgataaca taccagtcct ccaagacatc
    17341 cagtttcaca tcaaaaaagg gcagacaatt ggtctggtgg ggccaacagg ctctgggaag
    17401 accagcctac tcaagctcct catgcgagaa tacgatgtga caaatggaca gattttgctc
    17461 aatcagcaaa acataaagca ctacaagctc ggtgacctac ggcagttgat gggctatgtt
    17521 ccccaagacc agttcctctt tgcaaccagc gtggctgaga atatccgctt tggcaatcca
    17581 gacctctcac tagaccaggt gaaagaggct gccaaagctg tccatgtcta tgaggatatc
    17641 caggatatgc cagatcgctt tgacaccatg gttggtgaaa aggggatttc cctgtctggt
    17701 ggacaaaagc aacggttggc tatggctcgt gctatgattc tcaatccaga tattctactc
    17761 ttggacgatt cgctctcggc agtggatgcc aagacagagc acgctattct tgaaaccatc
    17821 aagcaagaac gccttgataa gaccaccatt atcaccgccc atcgtctgtc agctattgtc
    17881 catgccgatt tgattctggt ccttgaagat gggaaaatcg tagaacgagg tcgtcaccaa
    17941 gaattactag atgaaaaagg ctggtactac gatacctata tgatgcagca actcgaaaag
    18001 gaggaaagtg atgctctcta aaaccaagca agccagtgtt ttttggcgac tcttgtccta
    18061 tctcaaagcc tatcgactgc tgacagtcct tgccttgatc tttttattgc tgactaccgt
    18121 agtccgcagt ctaattcccc ttctagcctc ttactttata gaccactata tttcctcgat
    18181 ttctgagaca gctgcggcta ttctagcggc ctactttgcc ctctatctgc tccaaatggt
    18241 gcttcaatat tttggaaatc tctggtttgc aaaagtttct tacagcattg tcagagatat
    18301 tcgccgggat gcttttggga gaatggaaaa gctgggaatg gcttactttg accagactcc
    18361 tagtggctcc attgtctcgc gaatcaccaa tgatacagag agcatttcag agatgttttc
    18421 agggatttta tccagtttta tctcagctct ctttattatc atcacgactc tttcgaccat
    18481 gtttgctctg gattggaaat tgacctgtct gatcgtcctc tttcttccct tgattttctt
    18541 attggttgat caatatcgga agcggtcagc tcccattgtg gccaagacta gaagtctgtt
    18601 gagtgccatc aacagtaaac tggcagagag catcgagggg attcggatta tccaggcctt
    18661 ttctcaagag gagaggctaa aggaagagtt tgaggctatc aaccaggaac atctggacta
    18721 tgccagtcgt tctatggcct tggacagtct ctttctccga cctgccctgt cacttgttaa
    18781 gattttggcc tatgccctac tcatgaccta ttttggtttg gactggtcac aggctggaat
    18841 ttctgctggt cttatctatg ctttcatcca gtatgtcaat cgtcttttcg atcccttgct
    18901 ggatgtcacg cagcattttt ccacccttca aacctctatg gtatctgccg gacgggtctt
    18961 tacgctcatg gaccagacct ttgaggaacc gctacagctg gaccgagggg ccaagattga
    19021 caaggggcac attgtctttg aggatgtttg cttttcctat gacggcaagc ggcaaattct
    19081 ggacaaggtt tcctttgaag tgagacaggg gcagaccatc gcctttgtag gtgctacagg
    19141 gtcaggcaag tcttctatca tcaatgtctt tatgcgtttc tatgagttcc aatctggacg
    19201 gattttgatt gacggtcagg atattcgtag cttttcgcag gaagaattgc ggtccgcgat
    19261 cggcctagtc ctgcaagatc cctttctatt tcatgggacc attgcatcca atatccagat
    19321 gtatcaggat atttcccgag aggagattga ggaagcggct cgatttgtgg atgctgctcc
    19381 ctttattgag aaacttcctg agaaatatga ccacctggtt actgagcggg gtaccgcctt
    19441 ttcgacaggt caacggcaac ttctggcctt tgcacgcacc atggccagtc agcctaagat
    19501 tcttatttta gacgaggcta ccgccaatat cgattcggag acagagcaaa cggtccagac
    19561 ttctcttgag aaaatgcgaa aagggcggac aaccattgcc atcgcccacc gcctatcgac
    19621 cattcaggat gccgactgta tttatgtctt ggataaggga aagatcatcg agtcaggcaa
    19681 tcacgaagaa cttctagccc tcaagggtca ctaccaccgt atgtaccagt tacaagcagg
    19741 ccagttaaca gcaacctaga aaccactcca tgacaaaagc caagttccaa aatggacctg
    19801 gcttttttat ttttctacat tgttgagaac tgaagacatc aatctatctc tctttaactc
    19861 acattcgctg accgacgggc ataggctttc cagtcaatcc cacgcgcttc acaccagttt
    19921 tgtatatttg gagccaagac caaatctttc tgccggagtt ccgcaatatt cttagcaccc
    19981 agcatggtca tgatttcagc catctgccat tgataagtct taatagcctg caagccgtcg
    20041 tcaaaacgat gccccttacc atccttgaca tactgaagga aatggttgga cataccaaca
    20101 aggtctgcac cgagggccaa tgatttgaca atatctagtg gcgtcttaat accaccagat
    20161 gcaatcaggc ttgggcgaac ttcttcagaa acagacatgg cttctaccaa ggaagtcacg
    20221 gtagactgcc cccaaccttc tagataagta taatcattga aggtacggcg ggcattttca
    20281 atcttggcga aatccgtccc cccagtacca gacacatcaa tagtttgcac accgacagaa
    20341 gccaactggg cgacagtttc acggctcatg ccaaaaccaa cttccttgac gataacaggt
    20401 acttccatct cacgtacaag ggtttcaata tttttcaacc acatagtaaa gtcacggtct
    20461 ccctcaggca tgacaatctc ctggggggca ttgacatgga tctgaatggc atttgcctcc
    20521 aacaaatcca ccgcccgctt ggcattctca acactgtgat gggcacctag gttggcaaag
    20581 ataatcccgt aaggattttc ccgacgcata acagagaagg tttccgcaac agatggatcc
    20641 ttgatcgccg cactgaccga accagaagcc agagcaatct tcccaaaatg tcccataatt
    20701 cccaacagac ggttgatttc ccgcgtcttc ttgcttccac cggtcatggc attgatgaag
    20761 aaaggaaagg caaagtccaa accagctacg ctggtcgaaa tatccacttc atccactttg
    20821 gtttgaggca gggaatgatg gacaaacaag gtctccgtaa agtccttggc cggtgtggca
    20881 ctatattgct ggttggccaa gccaacgtgt tgatccttgc ggtctaaacc aaattctcct
    20941 agataaaaca tagggttctc cttattctct acttgcgatg tttagggtga gtggaaaaat
    21001 accggcttct tgccaagccg tctctatcgc tgctttttgt tccttactgt ctaccaagca
    21061 aatgccgcag tcaccaccac ctgcaccaga tgacttggcc accgccccat aggtctgggc
    21121 caggtcacag agttgactca gttgaggcgt ttcaatgacc aaacccattc ctctcgcaaa
    21181 gtcttgtaag agcttgcggt tctgagtaag ggcctgcctt actgacgctg agtcatttgt
    21241 ctgacaagct actatcaact gctctacaca gatcttagag tcggctagaa actggacatg
    21301 aatttgctct ttttctatct gacttttttg gctttccatc tgggaaacta gactatccgt
    21361 tgaagccgcc gaccctgtcc agccaaccaa gaggtctaag ccttggggta actggatagg
    21421 gctgatagac aaactttgcc aatcactttc caccagatcc agcagggaca gctctgccat
    21481 tttccctaaa agccaagaac ggtccagaga acgataggct atcaagccac caaaactgga
    21541 agccgccaaa tctccgaagg aacctgtcat gcctagcttg gtctgggcca aggctgccag
    21601 cttgtaggtc aggagggcat ctggtcggtg accgtagtag gtcaacaggg ccttgaccgt
    21661 cgcaaccgtt actgcccctg acgaccccag accatacttg gcacctgaag tctggtcatc
    21721 caggtcagac tggaccgcta gactgtaagt tccatgacaa gcatagccct tggctgtcag
    21781 ataggcttct gtcacctgca tgactgtctc aatcagagca tagggatggt caccttggac
    21841 atgagcgata ccttccttac gctcccaaat cagatagagg tctacttgct ggctggaatg
    21901 aagaaggcct cggtcacttg tttcaatggt gacggtcagg tactggtcaa ccgctgcaat
    21961 caccgcagga taaccggcct cgacgactgc gtattcccct gctagaaaga gttttccagg
    22021 tatctttact tgtactttca tgctacaagc ccttttcaaa cgctgcttgt gaggtctgcc
    22081 actcgtcttc actgaggacg taggcagcag gaccaggctt gctggtaatg attttctctc
    22141 ttgggaagac tttggccaat tcagctacta gctcatccat ttgacttgtt cggcagagga
    22201 ccttgacatt tggtcccgca tccatggtca tgtaggctga taagccagtt gcttcacgaa
    22261 cctgacggac tgcctcttga gccactaggc tatcggctga ccaataggta aagggtggat
    22321 tggcagagag cgttgttgca tgcattttca tgccattgtg ctctgtaatc tgaccaagtt
    22381 tttcaaaatc acggctggca atagctgcct tgatgtccac caagtcttgc ttagcggttt
    22441 ctacccaggc tgtgtaaaat ggtgaggtgg caactgtgtg gtccatgccc tctcgactag
    22501 caatcttttt cggtccggta ttgaccgcca agacgaccat gccgatatcc caatccgcat
    22561 cgtcaatggg gtgagccata gaatcttccg aaccagttcc catatcccac tcaacaaatc
    22621 caccaaatag gctacgggta ctagagcctg agcccctacg agcaagggtt gatagggtgg
    22681 ctggtgacaa atctaaatcc aaagcagttg cggttgcaag tgcaagtgct gcgaaagctg
    22741 atgctgagct tgccaaacca gccgcagtcg gaacaaaatt tagactttcc actcgggcaa
    22801 atgccctttc acctatatat tcgcaaaaca aatccaaaaa tcgagaaatt tttaaaattt
    22861 ctttttcttt ttgtaacatt ccgttgagat agaactcatc ggcagttaat tctgggtcaa
    22921 aaacgacctt ggtatccgta taaaatgcgt caagggtcaa ggataggcta gaattcatag
    22981 gtagaaataa ttccttatct cgttttcccc agtatttaat caaggcaata ttggtatggg
    23041 cacgggctat gcctgtttgt ttagtcattg gtttctccta agtactgtat ccaggtttgg
    23101 cgggcgcctg cttggtcaag gatgtgtgca agtttttggg catcctgagc tgttcttgcc
    23161 agggcaatca tgcagcctcc tcggccgcca ccagttaatt tggctccgag ggctccattt
    23221 tcttgggcaa ggctgacgag tttatctagg ctaggatcag acacgcctaa tttttgcagg
    23281 agggcatggg cttggttcat tctgctgcct agaagctctg cctggtctgt ggctaaatct
    23341 tccttggcct gtcgggtcaa gttacccagt tcttctacta gcttgatagc ctctggcttt
    23401 ttctccaaca aatcagccac gtctgaaatg gcttctaagg tgtttcccgt cacacctgtg
    23461 tcagccacta ccaaatgggc atggaatttc agctcaaaag gttcgatggg ctggtgcttg
    23521 ataaaaaaga cgggagactt gccgctggtg gtcgctgcat ctatgcctga tggattgccg
    23581 tgggcaattt tttctgaaga ctggacgatg tcccagagtt cgctgtcggt cagttctttt
    23641 ttatagtagg caaagagggc gcgtgctact gcaactgcca cggctgcact ggagcccata
    23701 ccgcgctcgg caggaatggt ggaactaatc tcgatatgga tagccgggtc tgtcggtgct
    23761 ccgatacgat agagcgagaa acggatggca tgcttgaggc tttcccagat ttttggcatc
    23821 ttgtgcacca agcctttata gaagtcacaa gcaacagtca gcgcttcccc ttgagcagtt
    23881 acctgagcaa taatttcaac agcagaaaaa ggcatagcaa ttgctggttg cccatagacc
    23941 accgcatgct ctcccatcaa aatgattttt ccatttgctt ttcctactac cgacatactt
    24001 ttctccttat acaatcacta ctattatacc atttttacat aagaatagaa cacacgaaaa
    24061 cagccgaccg cggtcgactg ttctctagga gattatatga aaaagaaagt tgttcgctat
    24121 ttctagcttg aatatagtat atagctacta acttaaacga aactgaaaga tttcttagat
    24181 ttttctaaag caatttctca tacttatttt cttaaaactt aacacgaaaa cagccgaccg
    24241 cggtcgactg ttctctggag attatatgaa aaagaaattc gctatttcta gcttgaatat
    24301 agtatatagc tacaaactta aaagaaactg aaaattttct tagccttatc ttaaattgat
    24361 tcttgttttg aatacctata ctgaaaaaat agcacccctg ctcggaaggc attatctaaa
    24421 atcgttgcaa tccaaatggc tgacaaaccg attttaaaaa atgttataag taaataagct
    24481 atgccaatcc gcactagcca cataccgata gttgtggcat aaaagggtaa tctagcattt
    24541 cccaaacctt gccaaagagc tgtcataata agagtgcctg ctgtaaaggg aactcccaac
    24601 atggcataaa ataatactgt ttgactagct tgtaccgctt gaatatcctt agtataaaga
    24661 ctgattagga aaggccccgc aaaataagtc acaccagcca ccaaaaacat aaacagtaaa
    24721 gaaaggccaa agcttgttcg aaaaatatgt cgaaccatag ccttatcctg tctgtgtttt
    24781 gctgtaagga tgattgtagc ggtagcaatc cctaaagccg gcatatagtt aaactgcgtc
    24841 agggtttctc caatcgcatt tccagctacc gtcgctgttc ctaagctggt aatcaacgcc
    24901 acgactacca catctcccac ccgcatcatc aaccgttcac cagttgctgg cagagcaagt
    24961 ctaagcagtt ctttatcaaa ggaccaggtc catggtttta aagaaattgg taagcgagac
    25021 caaagcaggc tacaaccaat caaccgcgat aagaccgtgc caatcgcaac tccagctaca
    25081 cctgcatgaa gcacaaagac tgcaaatgct gaaaatagtg catttagaac gtttgagagc
    25141 aggctgatat acataggaaa acgtggttcc cccaaggttc gtaagattgc actcaaactt
    25201 gccatgagtc cccaaaaagg tgctccccca ccaaccaata tcaaataaag acctccagcc
    25261 tgtaccactt ctgactctgc ccccaagcta gcaagtacta ttggacctgc cagaactgaa
    25321 aaaattccta aaaggagacc gataaaaact gtaaacttga cactttcact agcagttcct
    25381 ccaatgcctt ctgaaccccc ttcacccaaa gcctgagcca aacgagaaga caccgctgct
    25441 gctagggcaa taaaaaccgc ctgatagacg gctaaaatat tattagcaag ggaaactcca
    25501 gacaaggcta cgatgccaat actggctact agataagagt ctactacccc cattaacatc
    25561 tggagaaggt tttcccccat ggctggaaag gcaatggtta aaatttcttt ataggtttgt
    25621 ttgttcataa tctctatttc aactgtttaa tatctactat tttatcacaa accagattca
    25681 atagctcttc atcgtgagaa attaacaaaa ccaacttatt cttcattttt aagtctacta
    25741 aagcggaagc aacttgctgc attcgtctca aatccaaacc gctcgtcggt tcatcaaaaa
    25801 tgaaaatttc cttatctgcc agtaagctgg ccactatcat cacacgctgt tgttcaccgc
    25861 ccgataaact gaccggatgt ctctccaaca aatccgatag accaaaacga gctaccaaat
    25921 gttcatcgac agctctattt ttccttccaa gagtaagttc tcgctcaaca ctttccgcaa
    25981 atagttggag tcgaacatcc tgcataacaa aggctgtttt ttccaatctc tggcgactag
    26041 ataaggtttg accttgccaa gaaatcctac tcttcttatc ttccgctaaa ccgaccagat
    26101 aacgagcaag gcttgttttt ccagaaccat tcgctccaac cagactaatt gaacccggct
    26161 tcccaacctt ggcaatcaag agcatataga ctggaccagc taagagagct gctccagctg
    26221 gtgcatacat catattaccc gaacgatcaa aaaccaagct caacaaggtt cctagaccaa
    26281 cacaaacaaa gtagagggca gcaaaagccc ctgttaccat cagatctttt acttgtaatt
    26341 tcttcattta tttttccttt actaattgcc cgtcttccaa gtagaaaatg gtatccgcac
    26401 aagccatggt cgagcttcta tgagaaatca agaagaccat gccctgacag tgttccttaa
    26461 ccagttggat aaagcgggct tcattgagcg agtctaggtt actagttggc tcatcaaaga
    26521 tatagcaatc cgcatttttg agcagggccc gcatgagttc caaacgttgc ccttctcctg
    26581 ctgaaaaatc actggcctcc acaagcgtat ccaagccctg cggtgctgcc aaaatccgtt
    26641 ctttcatgtg gcatttttct gccaaatcca tgatggtttc atccgacaca tccgtccgtc
    26701 ccaagaggag attttcacga atggtctggc ggaagatttg tggcacctgt ggtacatagg
    26761 caaaagcccc ctgcaaatga gccttatcta cctgccgact atccaagcca gacaggcgga
    26821 tttggccttg ctgccaatca taccaccgca taattagttt catcaaggtg gattttcctg
    26881 caccggactc accgaccaag ccaataatac caccctgttc aaactggaca gacaggttcc
    26941 gatagagccc tgtttctcgg tcatcgtagt cgaagtccag ttgttcgata gcaatgtccg
    27001 tcactctgac ctcaacaagc tcacctgttc gctctgcttc tttttcatcc aaaagagcat
    27061 agatattgcg accagcattc atagcccgct tgaaaccaag aggcagacgg ctgagctcca
    27121 agaagggagc aaaggagcta gtgaaagaaa ccagcagggc tactcctgtt gctaagtcca
    27181 aactctgact acccaccaag tcgaaggtca gccaggcaaa gcacataata gatagtccta
    27241 ccactaaaaa ggaaacagca tactgcatga agttggtctg ggcaaccttt ctctcctgac
    27301 cattgacctc ttggcttttc tgtgtcagct tggcaaagcg agcatctgtc tgctggaact
    27361 gcatcaggtc cttcatgctc ttgagacttt caataaagta ggacacatag cccttacggc
    27421 tggcattttg ctccttgagc aagggctcta actgctgagc aaaacgattt ggaatggcaa
    27481 tcgccaacag agcataggtc accagagcca gcaaagccaa accccagctg gatagaccaa
    27541 aatagactgt caaaccaaaa gccaccaaaa tccctgtaca aattggggca atagtatggg
    27601 caaagaaaac ctccaaggcc tcaatgtcct ccccaatcat cttaagcagg ctaccgctgt
    27661 cctgccgatc caatttccca ggagccaggg cccgcagttt tgcaaaaatc aaacgacggt
    27721 aagccgccag cgtatgaaag gccacataat gaccaaagta atgctcacca tagcggaagg
    27781 caccacgtaa aagagctagg agaatgagga ttcccaaggt ccatagcgga gctggattgc
    27841 cagcaagggc tgcaaaagcc agatagacca gcgttactgg aatagcaatg gtcaccacct
    27901 gtcccaagac cgcaaagcaa accgccaccg caatccgtgg caagaggtgc ttcatggact
    27961 gcaagagtct cggaatcagc acccgtgtca gcatttcttt cttatccata aacggcttcc
    28021 tccaattctc tttgttgatc gaccagctga ccataagcca agctagtctg ataaagcgtg
    28081 gcatgattgc ctgtcacagc tccttcagca gacaggaaga ggatgtcatc tgcctgctcc
    28141 acttgtttca tcttatgtgt aataataata gcaatcgcat ccttgaccac caagtcaatg
    28201 aggtcgtaaa tcaagccttc attgtcttga tcgacgctgg acgtcacctc gtcaaagata
    28261 taaagggagc gttttgccaa aaccgcacgg gcacagataa cctactggcg ttgaccaggc
    28321 gacaagaaag caccgtcgtc accaacaacc gtatccaaac cgtctggcaa attcttgaca
    28381 aactgcaaga ccccatgctg atctgcccaa gccaagatgt cctccttaga ccagtcacaa
    28441 gccatgcgta aattttcata gattgactgg ttgagcaagg ttgactggcc cgacacatag
    28501 aggacctgct cattcagaga taactgactg acacctgaaa tctcctactc acccaagtaa
    28561 atcgctccct tatccgcccg caagcgtccc aagagcagct gggccaaggt cgttttcccc
    28621 tgccctgact cacctgccaa agcatagact ttaccagccg tcatggtcat agaaatatct
    28681 tttaagactg gcttttctcc gtagacaaag gccagatttt ccagtttcaa actatcaaaa
    28741 acaggcacat ggaaagaatc gatttcctgc tccgctgtca tactgtccaa gaaaccaaaa
    28801 atgcgatccg ccatcttggt attcatcatc accaagtgca taccgtagcc ctgctcccgt
    28861 atgggtgcaa aaaactccgt cgcaatgagg acaaagaaga gcatactaaa gagggacaaa
    28921 ttgcctgctg ctaggctatt gaccgccaca aaaccagaca agccaatccc cagatacatg
    28981 accgaatcca tgtagcctac cgcttgcaac tgaaagctga gtagctccat ggtcgcatcg
    29041 cggaagtcct cggcctgctc attgaaggtc ttttcgtagg tggcatccgc ctgataggaa
    29101 tagagggtgt tgagcccctt gaggtcgtcc aggaaaagat tgcccacatc catataggaa
    29161 ctccaatagc gattcataat ccgcttggac cgcttctgca tagcgataat ggaaatggga
    29221 atcagaggca aagccaagat gaaaataacc gcacccagcg ggaaaaccag aaaaaccagc
    29281 aaaaaaacag ttgcacagtt gaactgggtc cgcagagaag atgccatgta gtaggaatag
    29341 taggtatcca ggctatcaat cccctgggaa gcgatgttaa agacatccgc tgctgtcgct
    29401 tttgacgcaa actgaccgtc cttggccaag agggcctcaa agaaggactg cttgagcgaa
    29461 tcccgtgcaa actgggagcc caagccctgc aaacgcttgg caatcaaggc cacaccatat
    29521 ccaaaggcat tgagcccgag caagatgagg acaaactgta ctaaatcaac ctgcccgccc
    29581 tcatagtatg tcacaaagcc cttagcaatc aggtaaaagg aaataatccg catcaaaaac
    29641 tgcacccagg atagaaaggc tgccaaataa accagcttca tcttgggttt gatgcgttct
    29701 ttgagtcgct tcaagagg
//
LOCUS       DAZQCJ010000015        28803 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000015 DAZQCJ010000000
VERSION     DAZQCJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28803
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.015"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            24..257
                     /locus_tag="ACHU66_000613"
     CDS             24..257
                     /locus_tag="ACHU66_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFR3388919.1"
                     /translation="MSKKDKKIEIQIEDSKVLVNREEFPGYRLVIGKKVIGEIAELAE
                     NNFAVIKNGNTESFYKKLEKAVGNIIENYNLSH"
     gene            309..389
                     /locus_tag="ACHU66_000614"
     tRNA            309..389
                     /locus_tag="ACHU66_000614"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:343..345,aa:Tyr,seq:gta)
     gene            665..736
                     /locus_tag="ACHU66_000615"
     tRNA            665..736
                     /locus_tag="ACHU66_000615"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:697..699,aa:Gln,seq:ttg)
     gene            860..2065
                     /gene="rpsA"
                     /locus_tag="ACHU66_000616"
     CDS             860..2065
                     /gene="rpsA"
                     /locus_tag="ACHU66_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR3388920.1"
                     /translation="MNEFEDLLNSVSEVTPGDVVTAEVLTVDAGQANVAISGTGVEGV
                     LTLRELTNDRDADINDLVKVGETLELLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKVTRAVKGGLAVEFEGLRGFIPASMIDSRFTRNTERFVGQEFDAKIKE
                     VDPAENRFILSRRDVVEAEAAAARAEVFGKLAVGEVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSAVTVGEEVEVKVLAIDEVEGRVSLSLKATQPGPWDGVEQKL
                     AAGDVIEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDALSVGQEVTVKVL
                     EVNAADERVSLSIKALEERPAAVEGEEKAEKRAPRPRRQKREEKRDYELPETQSGFSM
                     ADLFGDIEL"
     gene            2103..2174
                     /locus_tag="ACHU66_000617"
     tRNA            2103..2174
                     /locus_tag="ACHU66_000617"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2136..2138,aa:Arg,seq:ccg)
     gene            2236..3264
                     /locus_tag="ACHU66_000618"
     CDS             2236..3264
                     /locus_tag="ACHU66_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HFR3388921.1"
                     /translation="MLHFENDYNKGAHPALLEALVASNQEGLSGYGTDKYTESAIEKI
                     RLATDCPHAQVTFLAGGTQTNQIVISSMLASYEGVIAADTGHIAVHEAGAIEFTGHKV
                     LALPHTDGKLVASEVENYISDFYADANHAHMVHPGMVYISHPTEYGIIYSKTELEDLS
                     KVCQQHNIPLFLDGARLGYGLAARETDLDLKTIAELTDVFYIGGTKLGALMGEALVFT
                     KNNQPKNFVSIVKHHGALLAKGRVTGVQFDCLFTDDLYLKLGRHTIAMAEQLTQILEE
                     KGFQFYLKSPTNQQFVIVKNEELTRLTEAGIAYGFWEKYDDSHSIIRFATSWSTSQAD
                     IDELINIL"
     gene            complement(3283..3666)
                     /locus_tag="ACHU66_000619"
     CDS             complement(3283..3666)
                     /locus_tag="ACHU66_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388922.1"
                     /translation="MKYVILKTPVFVWLLIFALIQIFALSSFLPEPFDTRSLLPMGVL
                     TGCSIRVSSEVKDQDGQYAPFKYLWWVILGWTVIGILFGITIKAPLELHIALIVHAIA
                     GGIGYSLIERYAVYRYHYLQTKKKG"
     gene            complement(3727..4143)
                     /locus_tag="ACHU66_000620"
     CDS             complement(3727..4143)
                     /locus_tag="ACHU66_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6556 family protein"
                     /protein_id="HFR3388923.1"
                     /translation="MNTRKNTNKKPAGLTNFQKYLTFIGSILGIITACITIYTFTAKP
                     TTSSTSPSTTISSSSATSSPSQSSSDNPLTGVQDLTSSSSETTSSTQTETSSSVESTA
                     SSSSTETSATQASETRTETSTDTEVTSGASETTSNQ"
     gene            4256..5398
                     /locus_tag="ACHU66_000621"
     CDS             4256..5398
                     /locus_tag="ACHU66_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFR3388924.1"
                     /translation="MIYFDNAATTQVYPEVLKTYTEVATKIWGNPSSLHSLGSQATRI
                     LEASRKQIAELLGKDSKEIFFTSGGTEGDNWVIKGVAFEKAHLGKHIIVSAIEHPAVK
                     ESALWLKTQGFEVDLAPVNAQGFVDVSALESLIRPDTTLVSIMAVNNEIGAIQPIQEI
                     SQLLADKPTISFHVDAVQAIGKVPTERYLTDRVDFASFSGHKFHSVRGVGFVYVKAGK
                     KIAPLLTGGGQESDKRSTTENVAGIAATAKALRLTLDKAVDSQKRLAAMKQILVDELG
                     KYDDVTVFSGLEDFVPSIVTFGIKNIRGEVIVHAFEDHQIYISTTSACSSKAGKPAGT
                     LIAMGVPQKLAQTAVRISLDDDNDMGQIEQFLTIFKQIYQNTQKVR"
     gene            5400..6617
                     /gene="thiI"
                     /locus_tag="ACHU66_000622"
     CDS             5400..6617
                     /gene="thiI"
                     /locus_tag="ACHU66_000622"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR3388925.1"
                     /translation="MNYSEIMIRYGELSTKGKNKMRFINKLRNNIKHVLSVYPEVTVY
                     FDRDRGHVYLNGADYQEVSASLKKIFGIQNFAPSYKIEKSVPALKEAVVEIMQSIYKE
                     GMTFKIAARRSDHNFELDSRDLNQVLGDAVFTAIPNVQVQMKSPDITLRVEIRPDAAY
                     ISHEEIKGAGGLPVGTSGKGTLMLSGGIDSPVAGYLALKRGVEIEALHFASPPYTSPG
                     ALKKAHDLTRKLTAFGGNITFIEVPFTEIQEEIKEKAPEAYLMTLTRRFMMRITDRVR
                     EERGAMVIINGESLGQVASQTLESMQAINAVTNTPVIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNVEQYEARMDVEGLVERAVAGIIVTEITPKEE
                     VKDEVDSLIEDLL"
     gene            6739..8064
                     /gene="brnQ"
                     /locus_tag="ACHU66_000623"
     CDS             6739..8064
                     /gene="brnQ"
                     /locus_tag="ACHU66_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFR3388926.1"
                     /translation="MKKGALTGLLLFGMFFGAGNLIFPPALGVLSGENFWPAILGFVV
                     SGVGIAVIALIVGTLNPKGYVHEISRKISPTFATIYLVALYLAIGPFFAIPRTATTSF
                     EIGIAPLLGEANLGIWLFGFTALYFVAAYLIALNPSQILNSIGRILTPVFAILIVILV
                     VLGIFKYGSTSPLPAAEAYSAGQAFGTGFIEGYNTLDALASIAFSVVAVNTLKHLGFS
                     SKKEYVSTIWSVGLVVALAFSALYVGLAFLGNHFPVPADVLASDTNKGVYILSQATQA
                     IFGPGAQIFLAAMVIVTCFTTTAGLIVSSGEFFAERFPRFSYKVYATIFTLIGFGIAN
                     LGLNNIITFSVPVLLVLYPITICIVLIAIVNKFVPLSTYGMQLTVGVVTALSLVEVLA
                     GQFNWTAISKIISALPLAGQSLAWLLPALVGIVLSLFLPNKQESEVFEM"
     misc_feature    8132..8220
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            8256..8570
                     /gene="rplU"
                     /locus_tag="ACHU66_000624"
     CDS             8256..8570
                     /gene="rplU"
                     /locus_tag="ACHU66_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR3388927.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKLNVEAGQEVTFEEVVLVG
                     GEKTVVGTPLVAGATVVGTVEKQGKQKKVVTFKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            8617..8910
                     /gene="rpmA"
                     /locus_tag="ACHU66_000625"
     CDS             8617..8910
                     /gene="rpmA"
                     /locus_tag="ACHU66_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR3388928.1"
                     /translation="MLNLNLANLQFMAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTKIYPGANVGRGGDDTLYAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            9102..9251
                     /locus_tag="ACHU66_000626"
     CDS             9102..9251
                     /locus_tag="ACHU66_000626"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR3388929.1"
                     /translation="MFRGSGMGSNLVKLVIDDLANRNSKAFQIAVEEEDLGTWKLYKR
                     RIVYL"
     gene            9323..10237
                     /locus_tag="ACHU66_000627"
     CDS             9323..10237
                     /locus_tag="ACHU66_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR3388930.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISIRDLEKELG
                     FQIFSRTSSGTFLTQKGMEFYEKAQALVKGFDQFEHLYLQPEEGEKTFSISSQHYDFL
                     PPLITEFSRSHPQYPHFRIFESTTVQILDEVAQGYSELGIIYLNERNTKGIMQKLDKL
                     HLQAVDLSAFQTHIYLREDHPLTKKAEVDPADLVGLPTVRFTQEKEAYLYYSENLIDT
                     SDSSVVFDVTDRATLNGILERTDAYATGSGYLDDESVNGITVIPLKGKVDNRMVYVKR
                     ANDELSSCAKDFIETMQAYFDKKKDENA"
     gene            10230..10694
                     /gene="lspA"
                     /locus_tag="ACHU66_000628"
     CDS             10230..10694
                     /gene="lspA"
                     /locus_tag="ACHU66_000628"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR3388931.1"
                     /translation="MRKIGFPFLMVVLIGLDQFVKAWTVANIELDTVEPLLPGVISLA
                     YLRNYGAAWSILQNQQWFFTIMTIVAVTGLVWYYIKQIKGNIWTLFSLSLMIAGALGN
                     FIDRLRLGYVVDMFHLDFISFPVFNVADVCLTVGVGILFICIMKEESNGSKS"
     gene            10678..11571
                     /locus_tag="ACHU66_000629"
     CDS             10678..11571
                     /locus_tag="ACHU66_000629"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFR3388932.1"
                     /translation="MEVRVEVGGIRLDKALSDLTDLSRSVANEQIKAGQVLVNGQPKK
                     AKYSVQVGDVLTYQIPEVEEIDYVAEDIPLEIVFQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALLYHVKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHTKLAAELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKEAVTRFQVLERFGDYTLV
                     ELTLETGRTHQIRVHMAYIGHPVAGDEAYGPRKTLKGHGQFLHARTLGFTHPRTGEVV
                     EFTAEAPAIFQEALEKLRKAE"
     gene            11652..11990
                     /locus_tag="ACHU66_000630"
     CDS             11652..11990
                     /locus_tag="ACHU66_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR3388933.1"
                     /translation="METLPNCPKCNSEYVYEDGALLVCPECAYEWNPADVAEEESGPV
                     AIDANGNRLADGDTVTLIKDLKVKGAPKDLKQGTRVKGIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVKKI"
     gene            12038..12685
                     /locus_tag="ACHU66_000631"
     CDS             12038..12685
                     /locus_tag="ACHU66_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HFR3388934.1"
                     /translation="MKHQSILFYSRCLLAILAITGTALEIIKYGIGMLMYYTVQSNLL
                     VSLFAVYMVYAMSKGVDLQTSHFLRIKAAVTMSIMITCVVYHFMLAPLADDFWRLENL
                     LCHYIVPLYFLLDTLIVDRQQQYKWFDPIWWTLLPVLYMIFGLVNGLFIKIPIPDAKD
                     SPFAYFFLNVPKYGWTYVLTYAGVIFVAYLVCGFLLAGLKSINLKTSLSFGKNSQ"
     gene            12884..13405
                     /gene="pyrR"
                     /locus_tag="ACHU66_000632"
     CDS             12884..13405
                     /gene="pyrR"
                     /locus_tag="ACHU66_000632"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFR3388935.1"
                     /translation="MKTKEIVDDMTMKRAITRITYEIIERNKNLDNIVLAGIKTRGVF
                     IAKRIQERLKQLEGIDVPLGELDTKPFRDDMKVEDDTTNMTANVNDRDVILVDDVLYT
                     GRTIRAAIDNIVSLGRPARVSLAALVDRGHRELPIRADYVGKNIPTSRSEEIIVHMAE
                     IDGQDAVLLVEGA"
     gene            13461..14384
                     /locus_tag="ACHU66_000633"
     CDS             13461..14384
                     /locus_tag="ACHU66_000633"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFR3388936.1"
                     /translation="MTITNGKVSLKHLVTMETLSNEEVLGLIQRGIAFKRGEKVELDR
                     KYYASNLFFEDSTRTHKSFEMAELRLDMGMIDFDARTSSVNKGETLYDTILTMSALGV
                     DICVIRHSEVDYYKQLIDSPTIQTSIVNGGDGSGQHPSQSLLDLMTIYEEFGTFEGLK
                     IAIAGDITHSRVAKSNMQILKRLGAEIFFAGPEEWYAEEFDVYGQHLNIDDIVETVDV
                     LMLLRVQHERHDGDGGFSKETYNRLHGLTEERYKRLKDTAIVMHPAPVNRDVEIDDSL
                     VEAPKSRIVRQMQNGVFVRMAILEAIVNGKA"
     gene            14632..15711
                     /locus_tag="ACHU66_000634"
     CDS             14632..15711
                     /locus_tag="ACHU66_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFR3388937.1"
                     /translation="MSKRRLILENGTIFEGEAFGADIDVTGELVFSTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDFESIKPTCKGVVVSEWARRASNWRNQMTLDEFLK
                     AKKIPAISGIDTRALTKIIRQHGTMKATLANVGDSVEHLTDQLRATVLPTNNIQQVST
                     KTAYPAPGVGRSVVLVDFGLKHSILRELAKRDCNVTVVPYDTTAEEILALNPDGVMLS
                     NGPGNPDDVPEALDMIRGILGKIPIFGICMGHQLFAKANGATTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVAREDLPECLMITHEEINDKSVEGVRHKYYPGFSVQFHPDA
                     APGPHDASYLFDEFMELMDSFKKGQ"
     gene            16221..19400
                     /gene="carB"
                     /locus_tag="ACHU66_000635"
     CDS             16221..19400
                     /gene="carB"
                     /locus_tag="ACHU66_000635"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR3388938.1"
                     /translation="MPKRTDIKKIMVIGSGPIVIGQAAEFDYAGTQACLALKEEGYSV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKAGILDELGVELLGTKLSAIDQAEDRDLFKQLMEDLNQPIPESTIVTTVDEALEFAA
                     EIGYPVIVRPAFTLGGTGGGMCANEEELREIAENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDAADNALVVCNMENFDPVGIHTGDSIVFAPTQTLSDIENQMLRDASLSIIRALK
                     IEGGCNVQLALDSHSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     VNPVTGTTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVYHNEMPELSQVTDDQLVEKIVKAQDDRLFYLSEALRRGYTVEELAQ
                     LTKIDLFFLDKLLHILEIEQELATNFDNIDLLKKAKKYGFADRKIAELWGRTESYIRQ
                     LRTEHKIVPVYKMVDTCAAEFESATPYFYSTYEWENESIRSEKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTLEDVLNVIDL
                     EKPKGVIVQFGGQTAINLAEPLAKAGIPILGTQVADLDRAEDRDLFEKALKDLGIPQP
                     PGQTATNEEEALEAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPEH
                     PVLVDSYIVGRECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSKEVQA
                     TIADYTKRLAIGLDCIGMMNIQFVIKDETVYVIEVNPRASRTVPFLSKVTDIPMAQVA
                     TKLILGQSLAELGYQDGLYPESNQVHVKAPVFSFTKLAKVDSLLGPEMKSTGEIMGSD
                     LTLEKALYKAFEASYQHLAEFGNVVFTIADEDKEEVLGLAQRFYELGYGLLATAGTAA
                     FLTENGLAVTTVGKLGDDSRPDIPSLVRAGKVQAIINTVGKKRVADGDGQVIRSSAIE
                     GGIPLFTALDTAEAMVKVLESRSFMTQAI"
     gene            19464..20219
                     /locus_tag="ACHU66_000636"
     CDS             19464..20219
                     /locus_tag="ACHU66_000636"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR3388939.1"
                     /translation="MIVTTSLGMDEGLVYRARRIASELGIEYKERKKQSVGKMLGTYE
                     AVLVLYKDKLILEQRGGQVLFFHPDTAMLRIKSGRDPLLELLGKEKQSIIDCTMSLGS
                     DSIVLASAGHRVTALESSKLVHFIVSRGLQDFDSGLQEVNRAMKSIQTIWTDSLTYLK
                     EQKDKSVDVIYFDPMFSEEIKESQNLSGLSTLADRSRLTEEIVSEAKRVARKKLIIKA
                     HFRDQVFEEFGFKRHVRPNQKFHYGEIILEEEV"
     gene            20216..20863
                     /gene="pcp"
                     /locus_tag="ACHU66_000637"
     CDS             20216..20863
                     /gene="pcp"
                     /locus_tag="ACHU66_000637"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFR3388940.1"
                     /translation="MKIIVTGFDPFGGESINPALETIKSLPKTIAGAEIILVEIPTVF
                     DKAADVLEEKMAEHLPDAVLCIGQAGGRVDLTPERIAINQDDARIPDNEGQRPIDRTI
                     REDGQPAYFSTLPIKAMVEAIHRIGLPASVSNTAGTFVCNHLMYQALYLAEKQFPKTK
                     AGFLHIPFLPEQVVDKPGLASMSLNDIVRGVEVAIGAIVEYRDKEDIKKGGGSTH"
     gene            20863..21171
                     /locus_tag="ACHU66_000638"
     CDS             20863..21171
                     /locus_tag="ACHU66_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFR3388941.1"
                     /translation="MKNIMEARKRVEEMEKIFNRQLELNQSLSDNMAQLNQEQSTYLQ
                     LLDYYQSQTYMEDLDLSNKGYFNGIPCGVLSEDGVYNLLFDRTNLASQLRELADMLEQ
                     "
     gene            21296..21568
                     /gene="rpsP"
                     /locus_tag="ACHU66_000639"
     CDS             21296..21568
                     /gene="rpsP"
                     /locus_tag="ACHU66_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR3388942.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLL
                     AENSVTLKEERVLEWLAKGAQPSDTVRSLLSNAGVLKKFHEQKFSK"
     gene            21590..21832
                     /locus_tag="ACHU66_000640"
     CDS             21590..21832
                     /locus_tag="ACHU66_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFR3388943.1"
                     /translation="MDMIENLIIAIVKPLISQPDSLTIKIVDTPEFLEYHLDLDQSDI
                     GRIIGRKGRTISAIRTIVYSVPTSDKKVRLVIDEKE"
     gene            21908..23644
                     /locus_tag="ACHU66_000641"
     CDS             21908..23644
                     /locus_tag="ACHU66_000641"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3388944.1"
                     /translation="MNLIWTYLKKYPKWIALDLLGAFLFVVVNLGLPTFLARMIDQGI
                     TKNDVSQLYFWAGMMGLIVLLGIVGRVTLAYAAGKLTTNIVKDIRNDLYEKIQDYSHH
                     EYEQIGVSSLVARMTNDAFVLMQFSEMVLKLGIITPLMMIASVIMTLVTSPSLAWTVA
                     VAMPFLVFVIFYVATKTRPLSEKQQKRLDTINQYVRENLMGLRVIRAFTREEFQENRF
                     AEVNEEYTDISKKLFNLTGLTEPLFVQIIIAMIVAIVWFALDPLKTGSLQIGDLVAFI
                     EYSFHALFSFLLFANLFNMYPRMSVSSQRIQKVLDMPISISKNEEGITKSDTRGYLEF
                     ENVTFAYPGETESPVLHNISFKAKPGETIAFIGSTGSGKSSLVNLIPRFYDVTLGRIL
                     VDGVDVRRYNLKALRSKIGFIPQKALLFTGTIAENLKYGKMDASLAELHDATDVAQAK
                     EFIESKEEKFDTHLAEGGSNLSGGQKQRLSIARAIVKQPDIYIFDDSFSALDYKTDAI
                     LRSRLKEVTENATVLIVAQRVGTIMDADQIIVLNEGEIVGRGTHNELMESNEIYREIA
                     NSQLNRQSLTEE"
     gene            23653..25422
                     /locus_tag="ACHU66_000642"
     CDS             23653..25422
                     /locus_tag="ACHU66_000642"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3388945.1"
                     /translation="MKNRSVFTRVWDYLRRYQSSVFLAIFLKTISAVMNALEPFVLGL
                     IITELTKNLLDIANGVEGAQINYSYIAIMLAIYALRALLYEIGAYGSNYFMTKAVQGA
                     TKELRQDLSHKINKIPVSYFDKHQYGDLLGRFTSDVETVSNALQQSFLQLVNAVLTLS
                     LAIFMCFWLDVSLALVVITLVPVTYFGSKFVMGKSQPYFKQQADALGRLNGFVQENLT
                     GFNVLKLYGREEISTEEFRQITADLQEVGFKASFMSGLLMPLVHGFSNIAYVVVALLS
                     GLKVLAGTLTVGNMQAFVQYVWQISQPVQTLTQLAPQLQSAKSSLERIFSVLDELDEE
                     DANALELTESLTGQVSFEQVEFGYSEDKPLIRNFNLDVKPGEMVAIVGPTGAGKTTLI
                     NLLMRFYDVTSGAIKVDGQDIRNLSRQSYRSQFGMVLQDAWLYEGTIKENLRFGRLDA
                     TDEEIVEAAKAANVDHFIRTLPGGYNMEMNQESSNISQGQKQLLTIARALLADPAILI
                     LDEATSSVDTRLELLIQKAMKRLMKGRTSFVIAHRLSTIQEADKILVLKDGQIIEQGN
                     HESLLADKGFYYNLYQSQFSESK"
     gene            complement(25466..26389)
                     /locus_tag="ACHU66_000643"
     CDS             complement(25466..26389)
                     /locus_tag="ACHU66_000643"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR3388946.1"
                     /translation="MKHSRRARRSRTQKKQLVPMLLGIALLVVTLLSIFLIFLKQPSK
                     QQSDSKASTVETTTTSSVMTSESSEVQTEIIWEQQTEPIKLPILMYHAIHVMAPEEEA
                     NANLIVDPTTFESHLKALQDAGYYTLSPEEAYKVLTENVLPAGKKVVWLTFDDSLWDF
                     YSYAYPLLKQYQMKATNNVITGFTEYGQAGYLTLDQIKEMQTAGLSFQGHTVNHPDLE
                     YSSIEDQTNELTSSKAYLDSQLNQETIAIAYPGGRYSADTVALTEQAGYKLGVTTNNG
                     LASASDGLLTLNRVRILPTTTAENLLAEIAY"
     gene            26494..27780
                     /locus_tag="ACHU66_000644"
     CDS             26494..27780
                     /locus_tag="ACHU66_000644"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019782768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFR3388947.1"
                     /translation="MVVKIGLLGFGTVASGVPFLLEENQEKIEKAAHDSIEVAKVLVK
                     DEAEKDRLLAAGHDYHFVTNIDDILADEDIAIVVELMGRIEPAKTFITHALEAGKHIV
                     TANKDLLAVHGSELRALAEEKGLALYYEAAVAGGIPILRTLVNSLAADKVTRILGVLN
                     GTSNFMLTKMVDEGWTYEKALQTAQELGYAESDPTNDVEGIDAAYKAVILSQFAFGMT
                     VDFEHVAHKGISNITPEDVAVAQELGYVIKLVGDIQETTSGIAAEVSPTFLPKNHPLA
                     SVNGVMNAVFVESIGIGQSMYYGPGAGQKPTATSVMADIIRIVRRLKDNTIGKAFNEY
                     SRSLQLAAPSDVKSEYYFSIDAPDKNGKMLRLAEIFNSEEISFKQILQQAADGETARL
                     VIVTHEMSKTQLENVTDKLSKETDFTVLNTFKVLGE"
     gene            27784..28644
                     /gene="thrB"
                     /locus_tag="ACHU66_000645"
     CDS             27784..28644
                     /gene="thrB"
                     /locus_tag="ACHU66_000645"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR3388948.1"
                     /translation="MKIIIPATSANIGPGFDSVGVALSKYLTIEVFEETDEWVIEHNL
                     EHVPSDKNNLLIKTALKIEKGLQPHRIRMISDIPLARGLGSSSSVIVAGIELANQLAG
                     LNMTADEKLLKATEIEGHPDNVAPAIFGNLVISSYVNKKVQAVVTEFPEASFVAFIPN
                     YPLRTVESRGVLPSQMGYKKAVAASAIANVAVASLMAGDLEKAGKAIQSDMFHEPFRQ
                     LLVKEFCPIKQTAQELGAYATYLSGAGPTVMVLAPKDREDAIVLALEELNLDGTVHRL
                     QVDTKGIAIV"
BASE COUNT         8226 a         5340 c         6700 g         8537 t
ORIGIN      
        1 aatagtaaaa gtgaggtttg aaaatgagta aaaaagataa aaaaattgaa attcagattg
       61 aagatagcaa agttcttgtc aatagagaag agttccctgg ctaccgcctt gttataggta
      121 aaaaagtaat tggagaaatt gcagaattgg ctgaaaataa ctttgcagtt attaaaaacg
      181 gaaataccga aagcttttac aaaaaattag aaaaagcagt cggaaatatt attgaaaatt
      241 ataatttaag tcactaaaag gcttgtaata gtcgctattt tatgttagaa tagtgactgt
      301 tagtgcacgg agagatagcg aagaggctaa acgcggcgga ctgtaaatcc gctccttcgg
      361 gttcgggggt tcgaatccct ctctctccat tgaacaggat acaatggacg ttaagaaatc
      421 cgtatgaaaa taggaaactg acgtagtgtg ctaaacacac aaggaagttt atctttttta
      481 ctaggatttt agtccgtgtt caactaagat acgagagatg ttctggttta ttagaaattt
      541 ctgtagaaaa ttaggagact gacgctgaat gttaacactc aaggaaggct atctattttc
      601 ctaagaaatt aatctcgagt tcaatttctt gtgattagta aataaatgaa ttgtatctat
      661 tttttggggt atcgccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg
      721 aatccagcta ccccagtaaa ggtactagat atagtctagt tcgtcaaaga tgtctaattg
      781 ttgttgtcct tgcgtgtgcc ttaaataaaa tatattttat gttgggtcga aagacctgat
      841 tgtcggaggt ttttttataa tgaacgaatt tgaagatttg ttgaacagtg taagcgaagt
      901 tacacctggt gatgtggtaa ctgcagaagt attgacagtt gatgctggtc aagcaaacgt
      961 agctatctca ggtactggtg ttgaaggcgt attgactctt cgtgagttga ctaacgaccg
     1021 tgatgctgat atcaacgacc ttgtaaaagt tggtgaaaca cttgaattgt tagtacttcg
     1081 ccaagttgtt ggtaaagata cagacactgt tacttatctt gtatctaaaa aacgtttgga
     1141 agcacgtaaa gcatgggaca agcttgttgg tcgtgaagaa gaagttgtta cagttaaagt
     1201 aactcgtgcc gttaaaggtg gtcttgcagt tgaatttgaa ggtcttcgtg gtttcatccc
     1261 agcttcaatg atcgacagcc gtttcactcg taacactgaa cgttttgtag gtcaagaatt
     1321 tgatgctaaa atcaaagaag ttgatcctgc tgagaaccgt ttcatcttgt cacgtcgtga
     1381 cgttgttgaa gcagaagctg cagctgcacg tgcggaagta ttcggtaaat tggcagttgg
     1441 tgaagttgta actggtaaag ttgcacgtat cacaagcttc ggtgcattca tcgaccttgg
     1501 tggtgtagat ggtttggtac acttgactga attgtctcac gagcgtaacg tatctcctaa
     1561 atcagctgta acagttggtg aagaagttga agttaaagtt cttgcaatcg atgaagttga
     1621 aggtcgcgta tcattgtcat tgaaagctac acagcctggt ccatgggatg gcgttgagca
     1681 aaaattggca gctggtgatg taatcgaagg taaagtaaaa cgcttgactg acttcggtgc
     1741 tttcgttgaa gtattgccag gtatcgatgg tttggtacac atttcacaaa tttcacacaa
     1801 acgtgttgaa aatccaaaag acgctttgtc agtaggtcaa gaagtaactg ttaaagttct
     1861 tgaagtgaac gcagctgatg agcgtgtatc actttctatc aaagctttgg aagagcgtcc
     1921 agcagcagtt gaaggtgaag aaaaagctga aaaacgtgct ccacgtccac gtcgtcaaaa
     1981 acgtgaagaa aaacgtgatt acgaattgcc agaaactcaa tctggattct caatggctga
     2041 ccttttcggt gacattgaat tgtaattcat ataaaaactt gccttgtgca agtttttcct
     2101 ttccaccctt agtgtaatgg atatcacgta agattccggt tcttgagatg ggggttcgat
     2161 tccctcaggg tggactaaat tgaaaagcct gcattaacag gcttttttgt tatattatat
     2221 caaggaggtt atgatatatt acattttgaa aatgactata acaaaggtgc ccatcccgcc
     2281 ttgctagaag ccttagttgc aagcaaccaa gaaggtttat ctggatatgg cactgataaa
     2341 tatactgaat ctgctatcga aaaaatcaga cttgctacgg actgtcctca tgcacaagtt
     2401 acgtttttag caggtggcac gcagaccaat caaattgtca tttcgtctat gttagcttcc
     2461 tatgaaggag tcattgcagc ggatactggt catattgcag ttcatgaagc gggggcaatt
     2521 gaatttacag gccataaggt gttggcctta ccgcatacgg atggcaaact tgtcgcaagt
     2581 gaagttgaga actatatttc cgatttctac gcagatgcta atcatgctca catggttcat
     2641 ccaggaatgg tttatatttc ccacccgact gaatatggta taatttatag caaaacagag
     2701 ctagaagatt tgtccaaagt ttgccaacag cataatattc cgctcttttt agacggtgct
     2761 cgcttgggtt atgggcttgc tgctcgtgaa acggatcttg atttgaagac aattgcagag
     2821 ctgacagatg ttttctacat tggtggtaca aaactcggtg ccttgatggg ggaagctttg
     2881 gtatttacaa agaacaacca gcctaagaat ttcgtatcga ttgtcaaaca ccatggtgcc
     2941 ttgttggcga aaggaagagt gacaggtgtc cagtttgatt gcctatttac tgatgacttg
     3001 tatcttaaat tggggcgtca tactatcgca atggcagagc aactgaccca gattttggaa
     3061 gagaagggct ttcaattcta tctcaaatcc ccgaccaatc agcaatttgt tattgttaag
     3121 aatgaagaat tgactagatt gacagaagct ggaattgcct atggcttctg ggagaaatac
     3181 gacgatagcc acagtatcat ccgctttgcg accagctggt caactagtca agcggatatt
     3241 gatgaattga taaatatatt atagcaatca aaaaagaaag ggttagcctt tctttttagt
     3301 ttgtaaataa tgatagcgat aaactgcata acgttcaata aggctgtagc ctattcctcc
     3361 tgcgattgca tgtacaatca gggcaatgtg caattctagt ggtgctttta tagtgatacc
     3421 aaatagaata ccgattacag tccatcctag tatcacccac cataagtact taaatggagc
     3481 atactgacca tcctggtctt taacttcact ggaaaccctg attgaacaac cggtcaatac
     3541 tcccatgggg agcaatgaac gagtatcaaa tggttctggt aaaaatgacg ataaggcgaa
     3601 gatttgtata agcgcaaaaa tcagtagcca aacgaaaacc ggcgttttta gaatgacgta
     3661 tttcatattg cacacctcct gaaattagta tatcaaatcc aagaaagtaa aacaaatatt
     3721 tttgtgctat tgattagaag tcgtttcgga tgccccacta gttacctctg tatcagtact
     3781 ggtttctgtc cttgtctccg aagcttgggt agcagacgtt tctgtagacg aggaagaggc
     3841 agtagactct actgaagagc tggtctcggt ttgggttgag gatgttgttt cggagcttga
     3901 acttgttaaa tcttgtactc ctgttaacgg attgtcgctt gatgattggc tgggactact
     3961 agtggcgctg gatgatgaaa tggtagtact tggagatgtt gaggatgttg taggttttgc
     4021 tgtaaaggtg tagatagtga tgcaggcagt gataatccct aaaatagacc cgataaaggt
     4081 taaatatttt tgaaagttag ttaaaccagc cggtttttta tttgtattct tacgtgtatt
     4141 catgatgtac ctccaccttt agtttaagat aggaagttta aaactatctt aaagattgcc
     4201 gaaaggcatg aaaacatgat aaaataggac tattgcaata aaaggaatat aaaaaatgat
     4261 ttattttgat aatgcggcaa cgacacaggt ctatccagaa gttctcaaga cctatacgga
     4321 agtggcgaca aaaatctggg gaaacccctc tagcctccat agtctaggca gtcaggcaac
     4381 ccgtattttg gaagcatctc gcaagcaaat cgcagaatta ctaggcaagg atagcaaaga
     4441 aatctttttc acctcaggtg gtacggaagg tgacaactgg gtcatcaagg gagtagcatt
     4501 tgaaaaggca catctgggca agcacatcat cgtgtcagct attgagcatc cagcggtaaa
     4561 agagtctgca ctttggctca aaacgcaagg ttttgaggtg gatttggctc cggtcaatgc
     4621 ccaaggtttt gttgatgttt cagccttaga aagcttgatt cgtccagata ccacactggt
     4681 ttctatcatg gccgtcaata atgaaattgg tgctattcag cccattcagg aaatctccca
     4741 gctcttagct gataagccaa ccatctcctt ccatgtggat gcagtgcagg caatcggcaa
     4801 ggtgccaacg gaacggtatt tgacagaccg agttgatttt gccagttttt caggtcacaa
     4861 attccactct gtcagaggag taggtttcgt ctatgtcaaa gctggcaaga aaatcgctcc
     4921 gctattgaca gggggaggac aggaaagcga taaacgctca acaactgaaa atgtggctgg
     4981 tattgcagca acggctaagg cgctccgctt gaccttggac aaggcggtag atagccagaa
     5041 gaggctggcg gctatgaagc agattttggt ggatgaattg ggcaaatacg acgatgtgac
     5101 agttttttct gggctagagg actttgtgcc aagcatcgta actttcggga ttaagaatat
     5161 tcgtggcgag gtcattgtcc atgcctttga agaccaccag atttacattt cgacaacctc
     5221 agcctgctcg tctaaggctg gaaaaccagc tggaacactg attgccatgg gtgttccgca
     5281 gaagttggct caaactgccg tccgtatcag cttggatgat gacaacgata tggggcaaat
     5341 cgaacaattc ctcacgattt tcaaacaaat ttatcaaaac acacagaaag taaggtagaa
     5401 tgaactattc agaaattatg attcgctatg gcgaattgtc aactaaaggg aaaaacaaga
     5461 tgcggtttat taacaaactc cgcaataata tcaagcatgt cctttctgtt tacccagaag
     5521 tgaccgttta ttttgaccgt gaccgtggtc atgtctattt gaatggggcg gattatcagg
     5581 aagtttcagc ttcattgaag aagatttttg ggatccaaaa tttcgcaccg tcttataaga
     5641 ttgaaaagtc tgttccagct ttgaaagaag cggttgtgga aataatgcag tccatttaca
     5701 aggaagggat gaccttcaag attgctgcgc gtcgtagtga ccacaatttt gaattggaca
     5761 gccgtgacct taaccaagtc cttggagatg cagtttttac agctattcca aatgtacagg
     5821 tacagatgaa gtcaccagat attaccttac gagtggaaat ccgtcctgat gctgcttata
     5881 tttcccatga agaaatcaag ggggcaggcg gtctgccagt tggtacatct ggcaagggta
     5941 cgctgatgct atcaggtggt attgattcgc ctgttgcggg ctatttagca ctgaaacgtg
     6001 gggttgaaat cgaagccctg cactttgcaa gtccgcctta tactagcccg ggtgcactta
     6061 aaaaggccca tgatttgact cgtaaattga ctgcctttgg tggtaatatc acctttatcg
     6121 aagtgccgtt tacagaaatt caggaagaaa tcaaggaaaa ggcacctgaa gcttatttga
     6181 tgaccttgac acgccgcttc atgatgcgga ttacagaccg agtacgggaa gagcgtggtg
     6241 ctatggttat tatcaatggt gaaagtctag gacaagtggc aagtcagacc ttggaatcca
     6301 tgcaggctat caatgcggtg acaaatacac ctgttatccg tccagtcgtt actatggaca
     6361 agttggaaat cattgatatt gctcaagaaa ttgatacctt tgatatttcc attcagccat
     6421 ttgaggattg ctgtaccatc tttgctcctg accgtccaaa aaccaaccct aaaatcaaaa
     6481 atgtcgagca atacgaggct cgtatggatg ttgaaggctt ggtggaacga gctgtggctg
     6541 ggattatcgt aactgaaatc acaccgaaag aagaagtgaa agacgaagtg gatagtttaa
     6601 ttgaagactt actgtagaat tcaattgttt tggcatacat ttaaatccag ctattgctgg
     6661 attttttaat acgaatcgtt cggaaaaatc gaatttgtat gatataataa gacttcagaa
     6721 aaaaacaaag gagttattat gaaaaaagga gcactaacag gattactcct gtttggtatg
     6781 ttttttggtg caggaaactt gattttccca cctgcacttg gtgtcttatc tggtgagaat
     6841 ttctggcctg ctattttagg atttgtcgtt tcgggtgtcg gtattgcagt cattgccctg
     6901 atcgtcggta ccttaaaccc aaaaggttat gtacatgaga tttctcgcaa gatttcaccg
     6961 acttttgcaa cgatttatct tgttgccttg tatttggcta tcggtccttt ctttgccatt
     7021 ccgcgtacag caacaacgtc ctttgaaatt ggtattgcgc cattgttggg cgaagcaaac
     7081 cttggaattt ggttgttcgg ctttacagct ctttactttg tggcagccta cctgattgcc
     7141 ctcaatccat ctcaaatctt aaatagtatc ggacgtattt taacccctgt ttttgcgatt
     7201 ttgattgtga ttttggttgt gctgggtatc ttcaagtatg gttcgactag tccactacca
     7261 gcagcagaag cctattcagc tggacaggca tttggtacag gatttattga aggatacaac
     7321 acccttgatg cccttgcctc aattgccttt agtgttgtag cggtgaatac cttaaaacac
     7381 cttggtttct ccagcaaaaa ggaatatgta tctaccattt ggtcagttgg tcttgttgta
     7441 gccttagctt tctcagcact atatgtcggt ttggcatttc ttggaaatca tttcccggta
     7501 cctgctgatg tcttggcgtc cgataccaat aagggtgttt atattctttc gcaagcaaca
     7561 caggctattt ttggaccagg tgcacaaatt ttcttagctg ccatggttat cgttacttgc
     7621 tttacgacaa cggctggctt gattgtttct tcaggtgaat ttttcgctga gcgtttccca
     7681 cgttttagct acaaagtata tgcgacaatt tttactttga ttggctttgg gattgccaac
     7741 ctcggtttga acaatatcat caccttctca gttccagttc ttttggttct ctatccaatt
     7801 actatctgta ttgtcttgat tgccattgtt aacaaatttg taccgctttc cacttatggt
     7861 atgcaattaa ctgtgggagt agtgacagct ttgtcattgg tagaagtgtt agcaggacaa
     7921 ttcaactgga ctgctatttc aaaaatcatc tcagctctgc cattggcagg acaatcatta
     7981 gcatggttat taccagctct tgtcggaatc gtcctctctc tattcttacc aaacaagcag
     8041 gagagcgaag ttttcgaaat gtaagaaaaa tgatacaaaa cacttgcatt tgcaggtgtt
     8101 tttgttataa taggactgtt gactatgcac acatcctgtg caaccgcacg aatgccgttc
     8161 tttcgttgag agtagagtaa gtggttcgtc ccacggtact aggcgagtct tcacatcaaa
     8221 ttcgggcaag acccaaaaat cataggaggt gcataatgag cacatatgca atcattaaaa
     8281 ctggcggcaa acaagttaaa gttgaagtcg gtcaagctat ctacgttgaa aaattgaacg
     8341 ttgaagcagg tcaagaagtt actttcgaag aagtagttct tgttggtggt gagaaaactg
     8401 ttgtgggtac tccactcgta gcaggcgcta ctgttgttgg tactgttgaa aaacaaggta
     8461 aacagaaaaa agttgttacc ttcaagtaca aacctaaaaa aggtagccac cgcaaacaag
     8521 gtcaccgtca accttacaca aaagttgtta tcaacgctat caacgcttaa tcgcgtagct
     8581 tgaagcaatc attacagaaa ttcggaggaa taacatatgt taaacttgaa tcttgctaac
     8641 ttgcaattta tggcccacaa aaaaggtgga ggttcaacgt caaacggtcg tgattcacaa
     8701 gcgaaacgcc ttggtgcgaa agctgctgac ggccaaactg tatcaggtgg ttcaatcctt
     8761 taccgccaac gtggtacaaa aatctaccca ggagctaacg taggtcgtgg tggagatgac
     8821 actctttacg ctaaagtaga aggcgttgta cgcttcgaac gtaaaggtcg cgataagaaa
     8881 caagtatctg tttacccaat cgcaaaataa gtctgaaaat ggctttacaa taggctttcc
     8941 gagttgtttt cggagagtct tttttgtaat ttgattttgc tatttttaaa agtgtacgat
     9001 agaagtgatt taagacggca atatcttctc tataggataa aaatattttg ccatctgcac
     9061 tgttgcgctg cctataagtt ctacgttcag tagtagatga aatgttcaga ggaagtggta
     9121 tgggttccaa cttagtaaaa ttagtcattg atgatttggc gaacagaaat tccaaagcct
     9181 ttcaaattgc agttgaagaa gaggatttgg gaacctggaa gttatataag agaagaatag
     9241 tctatttgtg aaaacgcaag agttcagcaa attcttgctt tttttgatat aatggtagaa
     9301 gcagttttaa gaggtatcag gtatgaatat tcaacaatta cgctacgttg tagccattgc
     9361 caacagtggt acttttcggg aagcagcaga gaaaatgtac gtttcccagc ctagtttgtc
     9421 catttccatt cgtgatttgg aaaaagagtt aggttttcaa atttttagcc gaactagttc
     9481 aggaactttt ttgacacaaa aagggatgga attctatgag aaagctcagg ctttagttaa
     9541 gggatttgat cagtttgagc atctctattt gcaacctgag gaaggtgaga agactttttc
     9601 aatttcaagt cagcattatg attttcttcc cccgttgatt acggagttct ctcgtagcca
     9661 cccgcagtac cctcattttc gcatttttga atcgactaca gtacagattt tagatgaggt
     9721 tgctcaaggt tatagcgagc ttggtattat ttatttgaac gagagaaata caaaaggaat
     9781 catgcagaaa ttggataaac ttcacttgca agctgtggat ttgtctgcat ttcagactca
     9841 tatctacctt cgtgaagatc atcctttgac aaagaaagct gaggttgatc cggcagattt
     9901 ggtaggtcta cctaccgtac ggttcactca agaaaaagag gcttatcttt attattccga
     9961 gaacttgatt gatacgtcgg attcctcggt tgtgtttgat gtgacagacc gtgcgacttt
    10021 gaatggaatt ttggagcgta cggatgccta tgcaactggt tcgggctatc ttgatgacga
    10081 gagtgtcaat ggcattaccg taattcctct caaaggaaag gtagacaatc gcatggtcta
    10141 cgttaaacga gctaatgatg aacttagttc ttgtgccaaa gactttattg aaaccatgca
    10201 ggcatatttt gataagaaga aggatgaaaa tgcgtaaaat tggatttcca tttttgatgg
    10261 tagtattaat tggtttggac cagtttgtaa aagcttggac agttgccaat attgaattag
    10321 acacggttga gccacttctt ccgggggtta taagtttggc ctatttacga aattatggtg
    10381 cagcctggtc catcttacaa aatcagcagt ggtttttcac gataatgaca attgttgctg
    10441 tgacaggcct agtgtggtac tatatcaaac agatcaaggg caatatctgg actttattca
    10501 gtctgtcttt gatgattgct ggtgctcttg ggaattttat tgatcgtctt agattgggat
    10561 atgtggtgga catgtttcac ttggatttca ttagtttccc tgtatttaat gtagcagatg
    10621 tttgtctgac agttggtgta ggtattttat ttatttgtat tatgaaagaa gagagtaatg
    10681 gaagtaagag ttgaggttgg tggtattcgt ttggataagg cattgtcgga tttgacggat
    10741 ttgtctcgtt cggttgcaaa tgagcagatt aaggctggtc aggttttggt caatggacag
    10801 cctaaaaaag ctaaatatag tgtgcaggtt ggggatgtcc taacctatca aattccagaa
    10861 gtagaagaaa tcgactatgt tgctgaggat attcctttag agattgtctt tcaggacgag
    10921 gatgttgctg ttgtaaataa accacaaggg atggtcgttc atccgtcggc tggtcacacg
    10981 tctgggacgc tagttaatgc tctcctttat cacgtaaaag atttgtcagg catcaatggt
    11041 gtcttgcgac caggtattgt ccatcggatt gacaaggaca catctggctt gctcatgatt
    11101 gctaaaaacg atgatgccca tacaaagctt gctgctgaat tgaaagataa gaaatccctt
    11161 cgtaagtatt gggccattgt acacggcaat ctaccaaacg atcgcggagt aattgaggcg
    11221 ccgattggtc gttctgaaaa agaccgcaag aaacaagcgg tgactgccaa agggaaagaa
    11281 gcggtgacac gttttcaggt tttggagcgt tttggtgact acactttggt ggaattgacc
    11341 ttggaaacag gacgaactca ccaaattcgt gtccacatgg cttatattgg tcatccggtt
    11401 gcgggtgatg aagcttacgg cccaaggaaa acactaaaag ggcatggcca atttcttcat
    11461 gccagaacgc ttggatttac ccatccgaga actggtgagg ttgttgaatt tacggcagaa
    11521 gcgccagcta tttttcagga ggcacttgaa aaactgcgca aggctgaata atagtatgaa
    11581 aaccttatca tcttgcccat tttttcaaca aatgctatac tagaacatga aaaatgttaa
    11641 ggagagtaag aatggaaact ttaccaaatt gtccaaaatg taattctgaa tatgtctatg
    11701 aagacggtgc cctcttggtt tgccctgagt gtgcatacga atggaaccca gcagatgtag
    11761 ctgaggaaga aagtggacct gttgcaattg atgctaacgg taatcgtttg gcggacggcg
    11821 atactgtgac gcttatcaaa gacttgaaag tgaaaggtgc gcctaaggac cttaaacaag
    11881 gtacacgtgt taaaggcatc cgtatcgttg aaggcgatca caatattgat tgtaaaatcg
    11941 atggttttgg tgccatgaag ttgaaatctg aatttgttaa gaaaatctaa gagagaggac
    12001 tggttaacca gtcttttcct gtttttgaaa ggatattatg aaacatcaaa gtattttgtt
    12061 ttacagtaga tgcctgttag ccattttagc catcacaggt accgccttag aaatcattaa
    12121 atatggtatc gggatgctca tgtattatac ggtacaatct aatttattag tatctctgtt
    12181 cgctgtctat atggtctatg ccatgagtaa gggtgtggat ttacagacgt cacattttct
    12241 tcggataaag gctgctgtaa ccatgtctat catgattacc tgtgtagtct atcactttat
    12301 gttggcacct ttggcagacg atttctggcg acttgaaaat ctgctctgcc attatattgt
    12361 tcccttatat tttttgttag ataccctaat agtagatcgt cagcaacagt acaagtggtt
    12421 tgacccgatt tggtggacgc ttctgcctgt tctctacatg atttttggtc tagtgaatgg
    12481 tcttttcata aaaattccaa tcccagatgc taaggacagt ccttttgctt atttctttct
    12541 caatgtccct aagtatggtt ggacctatgt tttgacttat gctggagtca tctttgtcgc
    12601 ctatctagtc tgcggttttc tattggcggg actaaaatcc ataaacctta agacttcatt
    12661 gtcttttgga aaaaattctc agtaaaatcc gataatttct atattttttc agaaaaaaat
    12721 tgtcaaggct gagattttat gctatactct gcttaataaa tcctttaaaa cctgtccaga
    12781 gaggcagaca aggagtcgga tgttaagcag gttggttcta actagcctat attttcatag
    12841 tctccttgta taaggagact ttttctatac ttttggaggt cttatgaaga caaaagaaat
    12901 cgttgacgat atgactatga aacgggcaat cacccgcatc acttatgaaa ttatcgaacg
    12961 gaataagaat ttggataata ttgtccttgc agggattaaa acacggggtg ttttcattgc
    13021 aaagaggatt caggagagac ttaagcaatt agagggtatc gacgttccgt tgggagagtt
    13081 ggataccaag ccgtttcgag atgacatgaa ggtagaagat gatacgacaa atatgactgc
    13141 caatgtcaat gatcgtgatg taatattagt agatgatgtg ctttatacag gacgaactat
    13201 tcgtgctgct attgacaaca tagtgtcctt gggccgtcca gctcgtgtta gtttagcggc
    13261 acttgtggat cgtggacaca gggagttgcc tattcgagca gattatgttg gtaagaatat
    13321 cccaactagc cgttctgaag aaatcattgt gcacatggct gaaatcgatg gtcaagacgc
    13381 tgtccttctg gttgaaggtg catagtcgct tcgtttacaa attctcattc tacatctaga
    13441 tcataaaggt aggtatcatc atgacaatta caaatggtaa agtttcactc aaacacttgg
    13501 ttacaatgga gaccctctca aatgaagaag tattgggact cattcaacgt ggtattgctt
    13561 ttaaacgcgg tgaaaaggta gagttggatc ggaagtacta tgcttctaac cttttctttg
    13621 aggattcaac tcggacgcat aagtcttttg aaatggcaga gcttcgtttg gatatgggaa
    13681 tgattgactt tgatgctcgt accagctcag tcaataaggg tgaaaccttg tatgatacca
    13741 ttttgaccat gtcagcactt ggggtagaca tctgcgtgat tcgtcactcg gaagtggatt
    13801 attacaagca attgattgat agtcctacca ttcagacttc aattgtcaat ggtggtgatg
    13861 gttcaggtca acacccaagc caatccctac ttgatttgat gactatttat gaagaatttg
    13921 gaacttttga aggcttgaag attgccattg caggtgatat tacacactca cgggttgcta
    13981 agtccaatat gcaaattttg aaacgtctgg gagctgagat tttctttgct ggaccggagg
    14041 aatggtacgc agaggaattt gatgtctatg gtcagcattt gaatattgat gatattgtgg
    14101 aaacggtaga tgtactgatg ttgctccgtg tgcaacacga gcgtcatgat ggtgatggtg
    14161 gcttttctaa agaaacttat aaccgtttgc atggtttaac agaggagcgc tacaaacgct
    14221 tgaaagatac tgcaattgtt atgcatccgg ctcctgttaa ccgtgatgtt gagattgatg
    14281 attctctagt tgaagcaccc aaatcacgta ttgtccgtca aatgcagaat ggtgtatttg
    14341 tccggatggc gattttggaa gcaattgtca atggcaaggc ttaaaagtaa gctgttcaga
    14401 taccttagaa ctgagcagtt agaaatttgt tagacttatc ttatttaatg tcgcccagag
    14461 aggcgacgaa tagaaaggag aaggtacagg ttcgagtaag atttgatttt tatattcgaa
    14521 cacgccctaa ggactgtgtg aaaaagataa atatcgactt gacgcctata gcgtcggcgc
    14581 cgaatttcct attttcactt tgtcctttta acgggctttg tatcatagat tatgtcaaaa
    14641 agacgtttaa ttttggaaaa tggcactatt tttgaggggg aggccttcgg tgctgatatt
    14701 gatgtgactg gtgagttggt tttctcaact gggatgacag gttatcagga atccatcacg
    14761 gaccagtctt ataatggtca gattttaacc tttacctatc ccttggtagg aaattacggg
    14821 attaaccgtg atgatttcga gtcgatcaaa ccgacttgta agggagtggt tgttagcgag
    14881 tgggctcgtc gagcaagtaa ttggcgtaac cagatgactt tggatgaatt tttaaaggct
    14941 aagaaaattc cagcaatttc tggaattgac acacgcgccc taactaaaat tattcgtcag
    15001 cacgggacca tgaaggcgac attggcaaat gttggtgatt ccgttgagca tttgacagac
    15061 cagttgagag cgactgtttt gccaaccaac aacatccagc aagtatcgac aaaaacagcc
    15121 tatcctgcac ctggagttgg acgtagtgtt gttttggttg attttggatt gaaacattct
    15181 attcttagag agttagccaa gcgtgattgc aatgtgacag ttgtccctta tgatacgact
    15241 gctgaagaaa ttcttgccct caatccagac ggtgtcatgc tttcaaacgg tcctggtaac
    15301 ccagatgatg ttccagaagc tttggacatg attcggggca ttctcggaaa aattccaatt
    15361 tttggtatct gtatggggca ccaactcttt gctaaagcaa atggggcaac gacttataaa
    15421 atgaagtttg gtcaccgtgg tttcaaccat gctgttcgtg aaattgcgac aggtcgagtg
    15481 gactttacca gtcagaacca cggttatgca gtggctcgtg aagatttacc tgaatgttta
    15541 atgattactc atgaagaaat caatgacaaa tcagtagaag gggtacgcca caagtactat
    15601 ccaggtttct ctgttcaatt ccacccagat gctgcacctg gtccacatga tgccagctat
    15661 ttgtttgacg aatttatgga attgatggat tcgtttaaaa aagggcagta aggtttaact
    15721 aatatcgtgc taaactttac tgcgtcggtc agagcaagtc ggacttcatg ttaacgcatg
    15781 tgtcttccta cgcaaagtcc aatgggacct ttgctaactc tgacactagt ttagaatttc
    15841 aattattatc gaattgggat tctaaacgtc ggacttatct tatttaatgt cgctcagaga
    15901 ggcgacgaat agaaaggcaa gggacagatc aatggttgcg agcaactcat ttccctacgc
    15961 ctgcctctat ggaggtcagg ctatcccttg cactagctcg aaaatggatt aatgtcgaat
    16021 ccattttctc acgtcggaca attatttaat tctatccgga gagatagaaa atagaaagga
    16081 gagatacagg ttcgagtttc ctattcgaac acgccctaag gactgtgcga aaaagataaa
    16141 tgtcgtcttg atgcttgaag catcagtgtc gtgtttccta ttttcgcttt gtcctcttaa
    16201 cgggctttgt atcatacatt atgccaaaac gtacggatat taagaaaata atggtgattg
    16261 ggtctggtcc gattgtgatt ggtcaggctg cggagtttga ttatgctggg actcaggctt
    16321 gtttagcctt gaaggaagaa ggttacagtg tggtcttggt caattcaaac cctgcgacca
    16381 ttatgacaga caaggaaata gcagacaagg tttatattga gccaattacg cttgaatttg
    16441 tcacacggat tttacggaag gagcgtccag atgctctctt gccaactctt ggtggtcaga
    16501 ctggtctcaa catggccatg gaattgtcta aggctggtat tttagatgag cttggagttg
    16561 agttgttggg aactaaactg tctgcaattg atcaggcaga ggaccgcgac ctcttcaaac
    16621 aactcatgga agatctcaat cagcctattc ctgagtcaac gattgtgaca acagttgatg
    16681 aggctttgga atttgctgct gaaattggct atcctgtcat tgttcgacca gccttcacgc
    16741 tgggtggtac tggcggtggt atgtgtgcca atgaagaaga actacgtgaa atagcagaaa
    16801 atggtctaaa attgtcacca gtgactcagt gtttgattga gcgttccatt gctggtttca
    16861 aagaaattga atacgaagtg atgcgggatg cggctgacaa tgccttggtc gtatgtaaca
    16921 tggaaaactt tgaccctgtg ggaattcata caggagattc tatcgtattt gccccaacgc
    16981 agacgctttc ggatattgaa aaccagatgt tgcgtgatgc cagtctcagc attattcgtg
    17041 ctctcaaaat cgagggtggg tgtaatgtgc agttggcttt ggattctcat agcttcaaat
    17101 actatgtcat cgaagtcaat cctcgtgtat cgcgttcctc tgccttggca tccaaagcga
    17161 caggttatcc tattgccaaa ttggcagcaa aaattgcggt tggcttgacc ttggatgaaa
    17221 tggtgaaccc tgttacagga acaacctatg ccatgtttga gcctgctctt gactacgttg
    17281 tggcaaaaat tccacgtttc ccatttgata agtttgaaaa aggggagcgt cgccttggaa
    17341 cgcagatgaa ggcaactgga gaggtcatgg ccattgggcg taatatcgaa gaaagtctgc
    17401 tcaaggcctg ccgttctttg gaaatcgggg tataccacaa tgaaatgcct gaactcagcc
    17461 aagtgacaga tgaccaattg gttgaaaaga ttgtcaaggc acaggatgat cgtttattct
    17521 atctttcaga agcccttcga cgtggttata cagtagaaga actagctcaa ttgacgaaga
    17581 ttgatctttt cttccttgat aagctgcttc atatcttaga aatcgagcag gaattggcaa
    17641 cgaattttga taatattgat ttattgaaaa aagccaagaa atacggtttt gcagaccgca
    17701 agattgcgga actctgggga cggacagaat cctacattcg tcaactgcgg acagaacaca
    17761 agattgtccc tgtttacaag atggtagata cttgtgcggc tgagtttgaa agtgctacac
    17821 cttatttcta ttcaacttat gagtgggaaa atgagtccat ccgttcggag aaagaatctg
    17881 ttttggtgct gggttctggt ccaattcgta tcggacaggg tgtggagttt gactatgcga
    17941 cggtacattc tgtcaaggcc attcaggcag ctggctacga agccatcatt atgaactcaa
    18001 accctgaaac tgtgtcaact gacttttcgg tttcggacaa gctttacttc gagccgttaa
    18061 ccttggaaga tgttttgaat gtgatcgacc ttgagaaacc aaaaggggtt atcgttcagt
    18121 tcggtggcca gacggctatc aatttggcag agccattggc caaagcaggt attccaatct
    18181 tgggtacgca agtagcggat ttggatagag ctgaagacag ggatttattt gaaaaagccc
    18241 tgaaagacct aggaattcca cagccaccag gtcaaaccgc aaccaacgaa gaggaagctc
    18301 ttgaagcggc ccgcaaaatt ggtttcccgg tacttgttcg tccctcttat gtcttgggcg
    18361 gtcgtgctat ggagattgtc gaaaatgaag aggacttacg ttcttatatg agaactgcgg
    18421 tcaaggcttc gccagagcac ccagttttgg tggattctta catcgtcggt cgtgagtgtg
    18481 aagtggatgc tatttcagat ggtaaggatg tcttgattcc aggtattatg gagcatatcg
    18541 agcgcgctgg ggttcactca ggtgactcga tggccgttta cccgccacaa accttgtcca
    18601 aagaagtgca ggcaaccatt gcagactaca ccaaacgctt ggcaatcggt ctcgactgta
    18661 tcggtatgat gaatatccag tttgtcatca aggatgaaac ggtctatgtg attgaggtca
    18721 atccgcgtgc cagtcgtacg gtgccattct tgtccaaagt cacagacatt ccaatggctc
    18781 aggttgcaac caaattgatt ttgggacaaa gccttgcgga acttggctac caagacggtc
    18841 tttatcctga gagcaaccaa gtgcacgtca aggcaccggt cttctcgttc acgaaactag
    18901 ctaaagttga cagtttgtta ggaccagaga tgaagtcaac cggtgagatt atggggtcag
    18961 acttgacact ggaaaaagcg ctttataagg ctttcgaggc aagttatcag cacctggctg
    19021 agtttggcaa cgttgtcttt accattgcgg atgaagacaa ggaagaagtg cttggtctgg
    19081 ctcaacgttt ctatgaactt ggttatggcc tactggctac ggcaggaaca gccgcctttc
    19141 taacagaaaa tggacttgct gtaacaactg ttggtaaatt aggcgatgat tcccgtccag
    19201 atattcctag tcttgttcgg gcaggtaagg ttcaggctat tatcaatacg gttgggaaaa
    19261 aacgggtggc agatggtgac ggtcaagtca ttcgaagctc tgctattgaa ggaggaattc
    19321 cgctctttac ggccctcgat acagcagaag ctatggtcaa agtgctggag agccgtagct
    19381 tcatgacaca ggctatatag aagatactat caggatgagc tttgagttcg tcctgatttt
    19441 ttgatatact gaagagtatg aaaatgattg taacaactag tcttgggatg gatgaggggc
    19501 tggtctatag ggctagacgg attgccagtg aactgggaat tgaatacaag gaaagaaaga
    19561 agcagtcggt tggaaaaatg ttaggtacct acgaggctgt cttggttctt tataaggata
    19621 aattgatact agagcagagg ggtgggcagg ttttattttt ccacccagat actgccatgt
    19681 tgcggattaa gtctggaaga gatccgcttt tagaattact tggaaaagaa aaacaatcaa
    19741 taatagactg tactatgagc ttgggatctg atagcattgt gttagcaagt gccggtcatc
    19801 gggtaacagc cttggaaagt tctaagctag tgcactttat tgtgagtcga ggtttacagg
    19861 attttgatag tgggctgcaa gaggtcaatc gagccatgaa atctatccag acaatttgga
    19921 cagatagttt gacttatttg aaagagcaaa aagataagtc tgttgatgtc atttactttg
    19981 atcccatgtt ttcggaagaa atcaaggaat cacaaaatct ttctggcttg tctactttgg
    20041 ctgacagaag tcgtttaacg gaggaaattg tatctgaggc taaacgagta gctaggaaga
    20101 aattgattat aaaggctcat tttcgagatc aggtctttga agaatttggc tttaaacgtc
    20161 atgtcagacc caatcaaaaa tttcactacg gagaaattat tttggaggag gaagtatgaa
    20221 aatcatcgta acaggctttg atccctttgg tggcgagtct attaacccag ccttggaaac
    20281 aatcaaatct ctacctaaga ccattgctgg tgcagagatt attcttgtag agattccgac
    20341 tgtttttgac aaggctgcgg atgttttaga ggagaaaatg gcagaacatt tacctgatgc
    20401 ggtactatgt attggtcagg ctggtgggcg agttgattta actcctgagc gtatagccat
    20461 taaccaagat gatgcacgca ttccagacaa tgaagggcag cggccgattg atagaacgat
    20521 tcgagaagat ggtcagcctg cttatttctc caccttaccc atcaaagcaa tggttgaagc
    20581 tattcatagg attgggcttc cagcttctgt ttctaatact gctgggacct ttgtctgcaa
    20641 tcatctcatg tatcaagctc tttacttggc ggagaaacaa tttccaaaga ctaaggcagg
    20701 ctttctccat attcccttct tgccagagca ggttgtcgac aagcctggac tagcttccat
    20761 gtctttgaac gatattgtaa gaggagttga agtagctata ggagcgattg ttgaatatag
    20821 ggataaagag gatattaaaa agggtggtgg cagtacacac tgatgaaaaa tattatggaa
    20881 gcaagaaaac gtgtagagga aatggaaaaa atatttaaca gacagctaga gctgaaccag
    20941 tccctttccg ataatatggc acaacttaac caggaacaat cgacttattt acaattatta
    21001 gattattacc agagccagac ctatatggaa gatcttgatt tatctaataa gggttatttc
    21061 aatggtattc cttgcggagt attgagtgaa gatggagtct ataatttact gtttgaccgt
    21121 acaaatctag caagtcaact gagagaacta gcagatatgc tcgaacaata aattttttta
    21181 aaacctgtca agtttttttc ttgacaccct gtcaaagtgt ggtataatag aacatgtgct
    21241 aaatagctct gctatttcac cgaataaaaa tacaaagaaa agagaacatt taaaaatggc
    21301 agtaaaaatc cgtttgactc gtatgggttc taaaaagaaa cctttctacc gtatcaacgt
    21361 tgcagactca cgtgcaccac gtgatggtcg tttcatcgaa actgttggta cttacaaccc
    21421 acttttggct gaaaactcag taactcttaa agaagagcgt gtacttgagt ggttggcaaa
    21481 aggtgcacaa ccatctgata cagttcgtag ccttctttca aacgctggcg tattgaagaa
    21541 attccacgaa caaaaattct ctaagtaaga acagtaagcg agaacactta tggacatgat
    21601 tgaaaatctc attattgcga ttgtgaaacc tttgatttca cagcctgata gcttgacaat
    21661 aaaaattgtt gatacacctg aattcttgga atatcatttg gatttggatc aatctgatat
    21721 tggacgtatc attggtagaa aaggacgcac aatttctgca atcagaacga ttgtctattc
    21781 tgttccaaca agtgataaaa aagttcgttt agtgatcgac gagaaagaat agaaagtcac
    21841 cgtttggtgg cttttaacgt tgacaaaggg gcctagcccc tttttaacat atagaaagga
    21901 agaaatgatg aatctgattt ggacctattt gaaaaaatat cctaagtgga ttgccttaga
    21961 cttgttgggg gccttcttat ttgtagtggt aaacttaggc ttgccgactt ttttggctag
    22021 gatgattgac caaggaatta caaagaacga tgttagtcag ctctatttct gggcggggat
    22081 gatgggattg attgtcctgc tcgggattgt tggtcgagtg acacttgcct atgcagctgg
    22141 aaaattgact accaatatcg tcaaagatat tcgaaatgat ctgtatgaga aaattcagga
    22201 ctattctcat catgaatatg agcagattgg tgtttcttct ctagttgctc gtatgacaaa
    22261 tgatgctttt gttctcatgc aattctctga aatggtcttg aaactaggta ttattacacc
    22321 cttgatgatg attgcttctg tcattatgac cttggtaacc agtccgagct tggcttggac
    22381 agtagcagta gccatgccgt ttctagtttt tgttattttc tatgtggcaa caaaaactcg
    22441 tcctctctct gaaaaacaac agaaacgatt ggatactatc aaccagtatg ttcgtgaaaa
    22501 tctcatgggt ttgcgggtca ttcgtgcctt tacacgtgaa gagttccaag aaaatcgttt
    22561 tgcagaggtc aatgaggaat ataccgatat ttccaaaaag ctctttaatc tgactggatt
    22621 gacagagcct ctctttgtcc agattatcat tgctatgatt gtggccattg tctggtttgc
    22681 tttggatccc ttgaaaacag gcagtctaca aatcggtgat ttagtagcct ttatcgaata
    22741 tagtttccac gcgcttttct ctttcttgct ctttgctaac ttgtttaaca tgtatccacg
    22801 tatgtcggta tcgagccaac gaattcagaa agtcttggac atgcccattt ccatttctaa
    22861 gaatgaagaa ggtattacta aatcagatac tcgtggttat ttggaatttg aaaatgtaac
    22921 ttttgcctat cctggtgaaa cggaatcgcc tgttctccac aatatttcat ttaaggctaa
    22981 gcctggtgaa acgattgcct ttattggttc aacaggttct ggtaagtctt ccttggtcaa
    23041 cttgattcca cgtttctatg atgtgacact tggtcgtatc ttggtggatg gtgtggatgt
    23101 cagacgctac aatctgaagg cactccgcag caagattggc tttattccac agaaagctct
    23161 gctatttacc ggaactatcg cggaaaactt aaagtacggt aagatggatg ctagtcttgc
    23221 tgaacttcat gatgcgacag atgtggctca ggctaaggag tttatcgaga gtaaggaaga
    23281 aaaattcgac acccacttgg ctgagggcgg aagcaacctg tcaggcgggc aaaagcaacg
    23341 tttgtccatt gcacgtgcta ttgtcaagca gccggatatt tacatttttg atgattcttt
    23401 ctcagccttg gattataaga cggatgcaat tttgcggagt cgtttgaagg aagtgacgga
    23461 aaatgcgacg gtgctaattg tggctcaacg tgtcggaacc attatggatg ctgaccagat
    23521 tatcgtgctg aacgaaggtg aaatcgttgg ccgcggcacc cacaatgaat tgatggaaag
    23581 caatgagatt taccgagaaa ttgccaactc tcaacttaac cgtcaatcgt taacagaaga
    23641 ataggaggaa ttatgaagaa tagatcagta tttacccgtg tttgggacta tttgcgtcgt
    23701 taccaatctt ctgtattttt agccattttt ctaaagacca tcagtgcagt tatgaatgcc
    23761 ttagaaccct ttgtgctggg gcttatcatt acagaattga ccaagaattt gttggatatt
    23821 gctaatggag tagaaggagc gcagattaac tattcctaca ttgccatcat gcttgccatc
    23881 tatgcccttc gcgcccttct atatgaaatc ggtgcctacg gttctaacta ttttatgacc
    23941 aaggcagtgc agggagcaac taaggaacta cgtcaggatt tgagccacaa aattaacaag
    24001 attccagtat cttattttga caagcaccag tatggtgatt tactcggacg ttttacaagc
    24061 gatgtggaga ctgtttcaaa cgccctgcaa caaagttttc tccagctagt caatgccgtt
    24121 ctgaccctct ctctagccat tttcatgtgt ttctggttgg atgtatccct agcacttgta
    24181 gtcataacct tggttccagt gacctatttt ggttctaagt ttgtcatggg caaatcccag
    24241 ccatatttca agcagcaggc tgatgcccta ggtcgtttga acggctttgt ccaagaaaat
    24301 ctgactggtt ttaacgttct caaactctac ggccgtgaag aaatttctac agaagagttt
    24361 cgccagatta ccgcagattt gcaggaagtt ggtttcaagg ctagtttcat gtctggtttg
    24421 ctcatgcctc tagttcacgg cttctcaaac atcgcctacg ttgtcgtcgc cctgctatct
    24481 ggattgaaag ttcttgcagg tacattgacg gtgggtaata tgcaggcctt cgtccaatac
    24541 gtctggcaga tttctcagcc agtccaaacc ttgacccaat tggcaccaca gttgcagtct
    24601 gccaaatctt cccttgaacg catcttttca gtcctagatg agcttgatga agaagacgcc
    24661 aatgcgctgg agttgacaga aagtctgaca ggacaagtca gctttgaaca ggtcgaattt
    24721 ggctattcag aagacaagcc gcttatccgt aactttaatc ttgatgttaa gccaggtgaa
    24781 atggttgcta tcgttggacc aacgggggca ggtaagacaa cccttatcaa cttgctcatg
    24841 cgtttctatg atgtcacatc aggtgctatc aaggtagatg ggcaggatat tcgaaatctt
    24901 tcacgtcaat cctaccgtag tcaatttggc atggtcttgc aggatgcttg gctctatgaa
    24961 gggaccatca aggaaaacct gcgttttgga cgtctggatg cgacggatga ggaaattgtg
    25021 gaggctgcta aagctgccaa cgttgaccat tttatccgta ccctgcctgg tggttataat
    25081 atggaaatga accaggaatc cagcaatatt tcccaaggtc agaaacagct tttgaccatt
    25141 gcgcgtgcct tgttggcgga tcctgctatt ctgattcttg acgaagcgac ttcttcagtt
    25201 gatacccgtt tggaactctt gattcaaaag gctatgaaac ggttgatgaa agggcggaca
    25261 agtttcgtca tcgctcatcg cttatctacc attcaggaag ctgataagat tttggtcttg
    25321 aaagatggac agattatcga acaaggtaac catgaaagtc ttttggcaga caaaggtttc
    25381 tattataatc tttatcaaag ccagtttagt gaatccaaat aaaaaaacga actcccttgt
    25441 cagtgaagac aggggagttt agtgtttaat aggcgatttc agctagtaga ttttctgcgg
    25501 tggtggtcgg tagaattcga acacgattga gagtcagtaa tccgtcacta gcagaagcca
    25561 agccgttgtt agtagtcaca ccaagtttgt agccagcttg ttcagttaag gctactgtat
    25621 ctgctgagta tcgtccacca gggtaggcga tagcgatggt ttcctgattt aattggctgt
    25681 caagataggc tttagaggaa gtaagttcat ttgtttgatc ctcgatagaa ctatactcta
    25741 aatcagggtg gttgacggta tgcccttgaa aggagagccc tgcggtctgc atttccttga
    25801 tttgatcaag agtaaggtac cctgcttgtc catattcagt aaatcctgta atgacattgt
    25861 tggttgcttt catttgatat tgcttaagca atgggtaggc ataactataa aaatcccaga
    25921 ggctatcgtc aaaggttagc cagacaacct ttttgccagc aggaaggaca ttttctgtca
    25981 ataccttata agcctcttca ggtgataaag tatagtaacc tgcatcttgc aatgctttta
    26041 gatgactttc gaaagtcgtt ggatctacga taagattcgc attagcttct tcctcaggag
    26101 ccattacgtg aatggcatga tacatgagga ttggaagttt gataggttca gtttgttgtt
    26161 cccaaatgat ttctgtttgt acttcactac tttctgaagt cataacactt gaagtggttg
    26221 ttgtctctac cgttgaagct ttggaatcac tttgttgctt agatggctgt tttagaaaga
    26281 ttagaaaaat agatagtaga gtcactacta ataatgcgat gccaagtagc attggcacaa
    26341 gttgtttctt ttgtgtgcgg cttcgacggg ctcttcttga atgtttcatt atcttatctc
    26401 cataatcata tattaaaaca attatatcaa attttttgcc aaatagtcag aatatttgtt
    26461 ataatgggag aaatacattt taaaggagag cacatggtag ttaagatagg attattagga
    26521 tttggaaccg ttgcgagtgg tgtgccattt ttattagaag aaaatcagga aaaaatagag
    26581 aaagctgcgc atgatagtat tgaagttgct aaggttttgg tcaaggatga agcagaaaaa
    26641 gaccgtctct tggctgcagg tcatgactat cattttgtaa caaatattga tgatatttta
    26701 gctgatgagg acattgccat cgtcgttgag ctgatgggac gtattgagcc agcaaaaaca
    26761 tttattacac atgccttgga agcaggcaag catattgtaa cagccaataa ggatttactt
    26821 gctgttcatg gtagcgaatt gcgtgccttg gctgaagaga aaggtcttgc gctttattat
    26881 gaagctgcag ttgcaggtgg tattccaatc ttgcgtacct tggttaactc attggctgct
    26941 gacaaggtga ctcgtatcct cggtgtattg aatggtactt caaacttcat gttgactaag
    27001 atggttgatg aaggctggac ttacgaaaaa gctcttcaaa cagcacagga acttggctat
    27061 gcagaatctg acccaacaaa tgacgttgag ggaattgatg cggcttacaa ggctgttatc
    27121 ctcagtcaat ttgctttcgg gatgacagtt gattttgaac acgtagcaca caagggtatt
    27181 tcaaatatca cgccagaaga tgtagcagtg gcacaagagt tgggctatgt catcaaactt
    27241 gtgggtgata ttcaagaaac tacctctggt attgctgccg aagtatctcc aaccttccta
    27301 ccgaaaaatc atccacttgc aagcgtgaat ggtgtgatga atgctgtatt tgtcgaatct
    27361 atcggcatcg gccagtctat gtactatgga ccgggtgctg ggcaaaaacc gactgcaaca
    27421 agtgttatgg ctgacattat tcgtatcgtt cgtcgcttga aagacaatac tatcggcaaa
    27481 gcttttaatg aatatagccg ctcacttcaa ttggcagccc caagcgatgt gaaaagcgaa
    27541 tattatttct caattgatgc ccctgataaa aatggaaaaa tgcttcgttt agcagaaatc
    27601 ttcaattctg aagaaatttc cttcaagcaa attctgcaac aagctgcaga tggagaaaca
    27661 gcccgcctag tgattgtgac ccatgaaatg agtaagactc agttggaaaa tgtaacagac
    27721 aaattgagta aagaaacaga ctttactgtc ttaaatacct ttaaagtact gggagaataa
    27781 gcaatgaaaa ttattattcc agcaacttca gctaatatcg gtccaggatt tgattcagta
    27841 ggtgttgccc tttcgaaata tctaacgatt gaagtatttg aagagactga tgaatgggtg
    27901 attgagcaca atcttgaaca tgttccatca gacaagaaca atcttttgat caagacagct
    27961 ttaaagattg aaaaaggatt gcagccacat cgtatcagaa tgattagtga tattcccttg
    28021 gcacgtggtt tgggttcatc cagttccgtt atcgtagctg gtatcgaatt ggctaatcaa
    28081 ttagcaggtt tgaatatgac agcggatgaa aaattactca aagctacaga aattgaagga
    28141 catcctgata atgtagcccc agcgattttt ggtaatttag tcatctctag ctatgttaat
    28201 aagaaagtac aggcagttgt aacagaattc ccagaagcaa gttttgttgc ctttattcca
    28261 aactacccac tgcgtacggt tgaaagccga ggtgtactgc cttctcagat ggggtacaag
    28321 aaagcagtgg ctgctagtgc cattgcaaac gttgctgttg ccagtttgat ggcaggtgat
    28381 ctggaaaaag ctgggaaagc aattcagtca gacatgttcc atgaaccgtt tcgtcagcta
    28441 cttgttaagg agttttgtcc aatcaagcaa actgcacagg agttgggagc ctatgcaacc
    28501 tacttatcag gtgccggtcc aacagtgatg gtattagcgc ccaaagatag ggaggatgct
    28561 attgttctag cattagagga actaaatcta gacggaactg tccaccgcct tcaagttgat
    28621 accaaaggga ttgctattgt atagatagtg agaatctatc atttgcaaaa ctaaacaggt
    28681 ttattttatt ggagttcgcc atcgctacta tatgcggtag gaagggaact cctttttcta
    28741 ccatttgtca agtccatcaa ggtatttgac gaaaaatatt ttgagttcaa tagtcaaaat
    28801 aag
//
LOCUS       DAZQCJ010000016        28545 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000016 DAZQCJ010000000
VERSION     DAZQCJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28545
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.016"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            74..775
                     /locus_tag="ACHU66_000646"
     CDS             74..775
                     /locus_tag="ACHU66_000646"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HFR3388949.1"
                     /translation="MWNPKKLTVLKWFDILILTIILFGDGIINSTLQYIALQNQTTTL
                     QENLTFSTMDNYKALALQLVWLTIAIFYLLLRNFDFSIWKKHIFITPWVPLQAVALFI
                     FSALCLDIYNLVSYQFLASNTPSMFQLFPNIDLSLILYSLLNGFYEEIFFLNLCLLVN
                     PKYAKWAFLYSLIIRCSFHTYQGLISALGLGLILGTIFYLLYQKIKPKNLLPFFLAHA
                     AADIIGLTVLSYILY"
     gene            complement(1242..3104)
                     /locus_tag="ACHU66_000647"
     CDS             complement(1242..3104)
                     /locus_tag="ACHU66_000647"
                     /inference="COORDINATES: protein motif:HMM:NF040483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum opacification factor"
                     /protein_id="HFR3388950.1"
                     /translation="MQGAIHEARRILESENSGKRKHIVLLSQGESTFSYDLSDDVKSK
                     PTYKTIEENKVVHTNPLLPWPFTFDVTVRRANLLDDAKSLISFLNKVGITRFDKALDD
                     VSTIAGITKLIGIIAKDPLDYINLVDVDSSQLEEKNFNYEKQIGEGYHFRSFYERKVE
                     PLTFRNQIVSKIKHNLTTELKKAELPTNTLSYIAEKLGLTNTSEKAIDLFANSLVDYI
                     FYGRKNIFNNHNLSAQAEAKIATKNGIHVYAVDVTKDKAGEEKNKNKFDEYLKKMSGD
                     DKKFLSVDENVAEKFKDVLTKVTLVDTLEDNVEIVKEPKDPKVSIKKNSSTFWLSWIT
                     SSKTTITWNLDKEDLIKAYQTNQPLSLTYQLQLKDKKKKDSQKLVSTEISHEINDKVG
                     KKVYSSLKVSLNHKTKKVSVPVPIEPQVPDKPIDMPKVENETPKGTPETPMQEKPNVP
                     EATLPMQPESPKETGKPGNNVEPESLKPIEDSTDDSANKPDSTIPKVTDTPDLSRPMK
                     PESTEEIDKAENSDTPDSSKTIKDSDNSAPISTIKDKVNAETVANQVELAMNQKSRLS
                     ENMEKVSSVNRSNEKSHTLPSTGQADSPIYTLTALSLLVGAQVLYRTKRKQESE"
     gene            complement(3286..3936)
                     /locus_tag="ACHU66_000648"
     CDS             complement(3286..3936)
                     /locus_tag="ACHU66_000648"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388951.1"
                     /translation="MNHPKFTLRKLSIGLVSLGAGVALSGTLYPKNTVTVYAETPTLE
                     KTSETDSTSLIRSTAVVEDPEVAQANENKEGEVVDSSSTLDLPKSNAESASPEALTNE
                     NASESEVQVAENTSSSEEKATEKEERTVQYEEKEVDDYSTKVEKPTETVSSTVDKTSK
                     ELFTVSRTAEVDQEGVVTVTTQIVPKEIDKGAEIVVLVDTYKKMDEDAKKQLKITL"
     gene            complement(4505..6682)
                     /locus_tag="ACHU66_000649"
     CDS             complement(4505..6682)
                     /locus_tag="ACHU66_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910563.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HFR3388952.1"
                     /translation="MNSKIFSLRKSKMGLVSVAIAFLWIGTGMNMETAMAEETDATAL
                     ETQLESKESSLTNTVSENAEAEEVTDEVPSEEKKSEEMEDMEEELPFIENHLEVAPIQ
                     AGDQTISGNTTPGGYVAITIDGEAITSIENILEADDKGDFSYRLSKPLAHSQTVEISA
                     LPKQFWTLEADSEERKVVVRTNRHPEAYEIPAKRLEKTSNGMHQVFIEPVFEHTSKVI
                     GHTSVKGSVYLSINGSFVSDKTLIDPKDGRFEVTFSESLAGSKFKADDRLVLSFVSED
                     GQPVITNTIVKPLVKEKVSSQMTVKPLSSATSVLEGTTFPLGRVHLYNADTSEFIMEA
                     IADETGHYKIALPALQSEDKYYRLTHNQQEDLVSVHLDTVDGSSILLDKSVMASLATY
                     LQDADMDEATDEDPIIVPKLHNKKDYIVGRTIHLNAYVRMVSSIKGKQYPPVQVDELG
                     FFGFQIQDLQLPFEKGERIRFEIIDPVTNNIIASKEEVVGQYLEDEDVMDLPFQVEKV
                     TTDHGYISGKTAPDVMIELVSTQNGEEIIGKTSTDSTGRFEFDLGSRVLKNGETLSFR
                     AFDKEGEQVAWEVVTVQKGNGHRINKPDKKDEKEEQPSKEITKNIEQSDTLEQTTLPP
                     VRQTLTDKKVEQNAEPSKEETVSIFDDSKKDMPTKQEKMARTVRDKGTKGNVSVHDSG
                     ENTQVQSLPKTGEKTSLVANIMLSIILFLFALFIGKKKITESE"
     gene            complement(7284..8219)
                     /locus_tag="ACHU66_000650"
     CDS             complement(7284..8219)
                     /locus_tag="ACHU66_000650"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFR3388953.1"
                     /translation="MSKFLVFGHQNPDTDAIASSYGWAHLEREVFGRDAEAVALGTPN
                     EETAFALDYFGVTAPRVVESAKAEGVSQVILTDHNEFQQSIADIKEVEVAAVIDHHRV
                     ANFETANPLYMRLEPVGSASSIVYRAFKENGVTPPKEVAGLLLSGLISDTLLLKSPTT
                     HTTDPQVAAELAEIAGVNLEEYGLTLLKAGTNLASKSAEELIDIDAKTFGLNGNDVRV
                     AQVNTVDIAEVLERQAEIEATMTAASAANGYSDFVLMITDIVNSNSEILALGSNMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTDAFNA"
     gene            8357..10054
                     /locus_tag="ACHU66_000651"
     CDS             8357..10054
                     /locus_tag="ACHU66_000651"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFR3388954.1"
                     /translation="MKFSDYTYVRPDYETIKAQFSDLTDQLAQASDLETTRTLVAAVT
                     KLLNLVDTQYNLWMIRHTIDMNDEFYNEETKFWNEYSPLFEELTTNYYRVIVQTPYKA
                     ELADILPETFFMQAENKLKTFSSDVIPLFQKENELTDEYSKLIAGAEIDFQGQTYNLA
                     QMGPFGQSTDREVRKAASAATTAFFESKEADFDRVYDELVKVRTEIAHKLGFKDYVEY
                     GYLKMNRFDYNCDMVKVYREEILKHIVPIVQNLRERQAKRLQVPSLKHYDLNLEFLDG
                     NAVPQGDPDFILSQAQDMYRELSAETGEFFDFMIEHELLDLVAKPGKNSGGYCTYIPD
                     FKSPFIFSNFNGTSGDIDVLTHEAGHAFQVYRSRWIQSPEVVWPTYETCEIHSMSMEF
                     MTWPWMDRFFKEQVDKYKFTHLASALLFLPYGVLVDHFQHEVYEHPEMTPAERKATWK
                     KLQDLYLPDRDYSESEALNRGIFWYRQGHIFASPFYYIDYTLAQVCALQFWKRTQVDH
                     DENAWEDYIRICDLGGTKSFLQVVEAANLQSPFKEGALESTAKAAADWLNAVKDSDL"
     gene            complement(10163..10954)
                     /gene="pflA"
                     /locus_tag="ACHU66_000652"
     CDS             complement(10163..10954)
                     /gene="pflA"
                     /locus_tag="ACHU66_000652"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFR3388955.1"
                     /translation="MMTEIDYRKVTGLVHSTESFGSVDGPGVRFVVFMQGCHMRCQYC
                     HNPDTWDLVNPAATERTAEDVLNEALRFRMFWGKEGGITVSGGEATIQIDFLIALFTL
                     AKEKGIHTTLDTCALTFRKTERYLEKYSRLMEVTDLVLLDIKEMNPEQHRIVTGHSNK
                     TILECAQYLSDIGKPVWIRHVLVPNLTDRDEDLIELGKFVKTLKNVERFEVLPYHNLG
                     EFKWRELGRPYPLEGTKPPTKARVENAKALMETETYQEYLNRIKG"
     gene            complement(11071..12408)
                     /locus_tag="ACHU66_000653"
     CDS             complement(11071..12408)
                     /locus_tag="ACHU66_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR3388956.1"
                     /translation="MEDPGSQTIYLQLLILFLLTLFNAFFSASEMALVSLNRSRVEQK
                     AADGERKYIRLLQVLENPNNFLSTIQVGITFISILSGASLANDLGAVFAKWMGNSTTA
                     QTAGYWLALAMLTFVSIVLGELYPKRIAMNMKENLAVVTAPVIIFLGKIVSPFVWLLS
                     AATNLISRITPMNFDDADEQMTRDEIEYILTKSEKTLDAEEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIEDDTATIMAAILKQNFSRIPVYDGDKDNIIGLIHTKKILSEAFSN
                     GFDNLNIRRIMQEPLFVPETIFVDDLLTSLRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDKTEIFVREIADNTYIVQGNMTLNDFNEHFEMELESDNVDTIAGYYLT
                     GVGTIPSQDEKVSFEVDSKDHHLVLINDKVKNGRVTKLKLLITPLEKEETDKD"
     gene            12541..13320
                     /locus_tag="ACHU66_000654"
     CDS             12541..13320
                     /locus_tag="ACHU66_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3388957.1"
                     /translation="MIISMKNVHYKRQGKTMLEDINWEFKKGERWAILGLNGAGKSTL
                     LRILMAEFWKTSGDLTVLGVEFGKGDIPSLRTKIGVVGSFLAERFPIDLTAEQIVLTG
                     KYKSSILYREYGQAELDEAIDMLKTIKAEHLIGRAYASLSQGERQLLLIARSLMEEPA
                     ILILDEATVGLDLLARERLLHHIDYLCQLPTAPAIIYVTHHAEEITDNFTHVLLLKDG
                     MVLAQGPKEDILTPELLSDFYGNQVELIDLGEGRLFIKPIL"
     gene            complement(13336..13890)
                     /locus_tag="ACHU66_000655"
     CDS             complement(13336..13890)
                     /locus_tag="ACHU66_000655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR3388958.1"
                     /translation="MLRPLHMAHAFLDEILTDQDLAVDATMGNGHDTLFLAQRAGKVV
                     AFDIQEQALTTTAEKLEKAGLTNAQLVLTGHENLDQYVDECKAAIFNLGYLPSADKSV
                     ITLPATTLQAIEKVLNRLVVGGRLAIMIYYGHEGGALEKDAVLDFISQLDQTVFTAML
                     YKPLNQVNTPPFLVMVERLKSSSN"
     gene            14021..14665
                     /locus_tag="ACHU66_000656"
     CDS             14021..14665
                     /locus_tag="ACHU66_000656"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFR3388959.1"
                     /translation="MKTIILFFLGASIGSFLGLVIDRFPEQSIIAPSSHCNACKRRLK
                     AWDLIPVLSQLSTKSKCRYCKAKIPYWYLGLEFLAGLVVLLCHFQVLNLTETILILAG
                     LVLTIYDIKHQEYPFAVWLIFTFIALILSQLNWLFCGFLLLAYLTEKWQINIGSGDFL
                     YLASLALICGFTELLWIIQISSLLGLLVFAIFKPKSIPYVPLLFLSSIPIILCI"
     gene            14773..16233
                     /locus_tag="ACHU66_000657"
     CDS             14773..16233
                     /locus_tag="ACHU66_000657"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR3388960.1"
                     /translation="MTLFKDDSLTLHTDLYQINMMQVYFDQNIHNKHAVFEVYFRSQP
                     FKNGYAVFAGLERVVNYLNNLSFSQTDIDYLTELGYPQDFLDYLSQLEMTLTVRSAKE
                     GDLVYANEPILQIEGPLAQCQLIETALLNIVNYQTLIATKARRIKAVIEDEPLMEFGT
                     RRAQEMDAAIWGTRAAFIGGASATSNVRAGKIFGIPVAGTHAHALVQTYGDDYKAFKA
                     YAETHHNCVFLVDTYDTLRIGVPAAIKVAKEMGDKINFKGVRIDSGDMAYISKKVRQQ
                     LDDAGFTDAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVS
                     IEDEDGNMRDTIKLSSNAEKVSTPGKKQVWRITSKEKGKSEGDYITFADTDVTQMDNI
                     YMFHPTYTYINKTIENFDARPLLVPIFEKGKQVYELPSLAEIQTYANQEFDKLWDEYK
                     RVLNPQLYPVDLAQDVWDNKMNLINRIRKQTQTKSI"
     gene            16246..17070
                     /gene="nadE"
                     /locus_tag="ACHU66_000658"
     CDS             16246..17070
                     /gene="nadE"
                     /locus_tag="ACHU66_000658"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR3388961.1"
                     /translation="MTLQETIIKELGVKPSIDPKEEIRRSIDFLKDYLKKHPFLKTYV
                     LGISGGQDSTLAGRLAQLTMEEMRAETGDDSYQFIAIRLPYGVQADESDAQAALAFIQ
                     PDVSLTINIKESTDAMVAAVNANGLAMSDFNKGNAKARMRMIAQYAIAGERKGAVIGT
                     DHAAENITGFFTKHGDGGADILPLFRLNKRQGKQLLAALGADEKLYLKVPTADLEEDK
                     PGLADEVALGVTYNQIDDYLEGKTIDPQSQTIIEGWWNKTAHKRHLPITIFDDFWK"
     gene            complement(17944..18471)
                     /locus_tag="ACHU66_000659"
     CDS             complement(17944..18471)
                     /locus_tag="ACHU66_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFR3388962.1"
                     /translation="MRKFFQADGNLTMLGRQICKTLAGAYALAIVLLCFLPQTWYPQY
                     KDFSTPGIIQIGRLYLLPTPFNSIVNGDKVDSLADLGWIFLQNITNIFLLFPLIFLLL
                     FLREEWRSLRAVIRYSFCISLFIECTQLLLDLLIDAQRVFEIDDLWTNTMGGILAYFT
                     YSLIMRGIILSKQES"
     gene            complement(18468..19580)
                     /gene="rlmN"
                     /locus_tag="ACHU66_000660"
     CDS             complement(18468..19580)
                     /gene="rlmN"
                     /locus_tag="ACHU66_000660"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFR3388963.1"
                     /translation="MKPSIYAFSQANLVDWILENGEKKFRATQIWEWLYRSRVQSFAE
                     MTNLPKSLIEKLEEHFVVNPLKQRIVQESKDGTIKYLFELPDGMLIETVLMHQHYGLS
                     VCVTTQVGCNIGCTFCASGLIPKQRDLTSGEIVAQIMLVQKYLDERNQNERVSHIVVM
                     GIGEPLDNYDNVMTFLRVVNDDKGLAIGARHITVSTSGLAPKIREFACEGVQVNLAVS
                     LHAPNNDLRSSIMRINRKFPIEVLFEAIEDYIKVTNRRVTFEYIMLNEVNDGVEQAQE
                     LADLTKNIRKLSYINLIPYNPVSEHDQYSRSTKERTLAFFDVLKKNGVNCVVRQEHGT
                     DIDAACGQLRSNTLKKDREKARARIAAAKAKAGIRA"
     gene            complement(19589..20230)
                     /locus_tag="ACHU66_000661"
     CDS             complement(19589..20230)
                     /locus_tag="ACHU66_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFR3388964.1"
                     /translation="MKKEISPEMYNYNKYPGPSFARVGDKVVSENIELDLLEDYKNAF
                     DQTIFGQRFSQLMLKFDYIVGDWGNEQLRLRGFYTDDKNVKSDLKISRLDDYLTEYCN
                     FGCAYFVLANPNPQDLPAEEEDRPRRKRSRSRNRNRNQQRTELIEPKENKSNRNDRQK
                     KGKQQNKQSEQRHFTMKNAGVKASQTERSQKHERKQSKREMNEVKRNFVIRQK"
     gene            complement(20418..21467)
                     /locus_tag="ACHU66_000662"
     CDS             complement(20418..21467)
                     /locus_tag="ACHU66_000662"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFR3388965.1"
                     /translation="MKKNRKLTLIASIVLGTLLIWFAVFVRLPYYLESPGGAADIRQV
                     LTVNNQIDQEDGSYNFTYVSVQQATALQLFAAQFDPYTTVRSSEEMTGGADNEEYFRI
                     ARFYMETSQNMAKYQGLTLAGKEVNLDFFGVYVLALTDDSTFKKVLNIADTVVSINGK
                     TFESSPDLVKYVSGLEVGSDVTVGYISAGQEKSADGKIIKLSNGKNGIGITLVDHTEV
                     QSSVPIDFQTGDIGGPSAGLMFTLAIYTQLAEPDLRDGRIIAGTGTIEQDGKVGDIGG
                     ADKKVISAAKSGASIFFVPNNPVDVEVLKKNPKAKTNYEEAKEAAEKAGLDIEVVPVK
                     TVQEAIDYLKKTKGE"
     gene            complement(21454..21942)
                     /gene="coaD"
                     /locus_tag="ACHU66_000663"
     CDS             complement(21454..21942)
                     /gene="coaD"
                     /locus_tag="ACHU66_000663"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR3388966.1"
                     /translation="MSDKIGLFTGSFDPITNGHLDLIERASGLFDKLYVGVFTNPKKA
                     GLLTGLERKAILEKLFVGMENIEVVLSENELVVDVAKRYGVTHLVRGLRNATDLEYES
                     SFDFYNRQLAPDLETIYLIAKPELKFVSSSQVRELLYFKQDIGPYVPEIVSEEIRKNE
                     EK"
     gene            complement(21932..22471)
                     /gene="rsmD"
                     /locus_tag="ACHU66_000664"
     CDS             complement(21932..22471)
                     /gene="rsmD"
                     /locus_tag="ACHU66_000664"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFR3388967.1"
                     /translation="MRIVSGSYGGRPLKTLDGKTTRPTSDKVRGAMFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAISRGMESAVLVERDRRAQAIIRENIAMTKEADKFQLLAMEARQ
                     ALNSVGGPFDLVFLDPPYAEQEIEDVITELCRRDLLADDVMVVCETDKAVSLPEEIAE
                     LGIWKEKLYGISKVTVYVR"
     gene            complement(22781..23503)
                     /locus_tag="ACHU66_000665"
     CDS             complement(22781..23503)
                     /locus_tag="ACHU66_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR3388968.1"
                     /translation="MEKKFEKLSLAEIGIISFIGLILLISLGASWYKNAQSETSDTKT
                     VQQVPNQKKTVDTVFQDAENAVIVLEGTHTEEKLAEAQSAVNKVTDATKKAALQKRID
                     QVQELVGQQTDAQTQAKLKAELVRAEAAKKVADEKKVSETGTSQSTDLAEASETSQES
                     YVVPNKNIYGNQSVSSTSQYTSSVASTSTSTSVPPTSEGAPVVETPTVSETPAETTDS
                     TTSLVESVETVPTADESSPDNP"
     gene            complement(23560..23946)
                     /locus_tag="ACHU66_000666"
     CDS             complement(23560..23946)
                     /locus_tag="ACHU66_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR3388969.1"
                     /translation="METFWVFLVFLAVLGAWMGFNYWRLRRAATVIDNAEFAEKIHGG
                     QLIDLRDPSEFNRKHILGARNIPYQQLRQSTAALRKDRPVLIYENDRGQLVTPAALHL
                     KKQGFNEIYILSYGLNGWNGKVKTTK"
     gene            complement(23946..24164)
                     /locus_tag="ACHU66_000667"
     CDS             complement(23946..24164)
                     /locus_tag="ACHU66_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFR3388970.1"
                     /translation="MKRLYDVQQLLKRFGIIVYMGNRLYDIEMMQIELNRIYQAGVLD
                     RLEYMEAELVLRREHRLELEYQKSRENE"
     gene            complement(24269..24868)
                     /locus_tag="ACHU66_000668"
     CDS             complement(24269..24868)
                     /locus_tag="ACHU66_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFR3388971.1"
                     /translation="MRILVAEDQAMLRDALCQLLGFQDAVEAVLQAENGSQAIVLLEK
                     EPVDVLLLDIEMPEKTGLDVLEWVREKSLPVKVVIMTTFKRPGYFERAVKADVDAYVL
                     KERSISDLMRTIETVLAGRKEYSPELMDILLTQRSPLTEQECHLLKLVANGLSNKEIA
                     SQLYLSDGTVRNYMTSILAKLGAENRTAAVKIAQEKGWL"
     gene            complement(24865..25959)
                     /locus_tag="ACHU66_000669"
     CDS             complement(24865..25959)
                     /locus_tag="ACHU66_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR3388972.1"
                     /translation="MIFEKRKVPLMFFVGMIFLYFPLYYSQYTPNPTAVVLATILFAL
                     VYCSLFYTDHKLYSMLAWFYLIGYIAVMSFWGNLQFSLFIFNLSNMLGWYYKENRPTY
                     RTVSYGLLLLAIILWALFGPVPFEMRAFALVIHFFGLALLIFSWIETKREALQQRLQE
                     QNASINLLLAENERNRIGQDLHDTLGHVFAMLSVKAELVQTLLDHDQIDQAKKEVAEL
                     QTLAKDSMQDVRQIVQSLKQHTVAEELQILQQMLDLAGVDLVVLVGEIAEQLSLPVQN
                     KLAMVLRELANNLLKHSRASKCRLIFEEDQQELVLHYEDNGVGFAQLTGQELHTIKDR
                     LVTVQGSLEFLSLAQPTRLSIRIPLEEVVE"
     gene            complement(25971..26699)
                     /locus_tag="ACHU66_000670"
     CDS             complement(25971..26699)
                     /locus_tag="ACHU66_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR3388973.1"
                     /translation="MKALVQVEAVKLSRSLGVFLLSIGMPVIFFLIFSSTVQFDDAAM
                     QKAFIQSYMLTMTGFSMSGFALFTFPMMLAEDRKNSWLTFIQHSPLPIWQYYLSKLVR
                     VLLCFVSSIIIVFAVGGLVKDVEMTAQEWVISALLLLVTSIVFLAIGLLLVQIQSEQT
                     MSVVANLLYFVLAIMGGSWMPVSLFPDWVQRICKLLPSYHVNQLVTTYAQEGDFLWKS
                     LLIVLGYAIIFLGIALVMNRKTEQ"
     gene            complement(26696..27574)
                     /locus_tag="ACHU66_000671"
     CDS             complement(26696..27574)
                     /locus_tag="ACHU66_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3388974.1"
                     /translation="MIEVRALDKVIKGRTILSNISFEIKSGECVALIGPNGAGKTTLM
                     SCLLGDRKATKGQVLLDGLAPQAQSNKEKVAVLPQENAIPTDLKVKELLRFFQAIYKD
                     SLTDVEIDSLLCFSPEQKNQLAGKLSGGQKRLLAFVICLIGKPKLLFLDEPTAGMDTS
                     TRQRFWEIVHELKDQGVTIFYTSHYIEEVEHTAERILVLHQGRLLRDTTPFAMRSEEQ
                     EKEVTLPIAFAAVVESLAEINEISYKQDTVSFKTRAIEQVWASLQEAGCRISDLEVQN
                     KTLLNSLFDKTREEES"
     gene            complement(27934..28452)
                     /locus_tag="ACHU66_000672"
     CDS             complement(27934..28452)
                     /locus_tag="ACHU66_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFR3388975.1"
                     /translation="MMKQKYYQSPAEIASFSPRPSLADSKAVLNQAVADLSVAHLILH
                     QVHWYMRGRGFMIWHPKMDEYMEEIDGYLDEMSERLITLGGAPFSTLKEFSENSQLKE
                     VPGDYTVTIEEQLARVVEVFRYLAALFQKGFDVSDEEGDSVTNDIFNVAKASIEKHIW
                     MLQAELGQAPKL"
BASE COUNT         8219 a         6483 c         5219 g         8624 t
ORIGIN      
        1 agacgaaacc gagagcatat atagatttgt ttgataaact gaactaaaca aaaatgaaag
       61 gaactctccc attatgtgga atccaaaaaa attaaccgta ttaaaatggt ttgatattct
      121 cattctaaca attattttat ttggagatgg aatcatcaac tctactctcc aatacatagc
      181 cttacaaaat caaactacaa ctcttcaaga aaatttgaca ttttcaacaa tggataatta
      241 taaggctttg gctctacagt tagtttggct cacaatagcc atcttctatc tattactccg
      301 aaattttgat ttctcaattt ggaaaaaaca tatttttatt actccttggg ttccattaca
      361 agctgttgcc ttattcattt tttctgcatt atgcttggat atctataatc tagtatccta
      421 tcagtttcta gcttccaaca ctccatccat gtttcaacta tttcctaaca ttgatttatc
      481 actgatcttg tactcattgt tgaacggatt ttatgaagaa attttcttcc ttaacttgtg
      541 cttactggtt aaccctaaat atgcaaaatg ggcattttta tattcgttaa ttattcgatg
      601 tagtttccat acctatcaag gtttaattag tgcactcggt ttaggcttaa ttctaggaac
      661 tattttctat ctactttatc aaaaaataaa acctaaaaat ttattaccat ttttcctagc
      721 acatgctgca gctgatatta tcggtttaac tgttctatca tatattctat actagttatc
      781 taacagataa tttgaacaca agctatcctc atatcaccaa agactgagtg gacccctact
      841 agtcatgacc acccgtgaaa acgggtggtt tattttgtgt aatttagaga aaagagtgcc
      901 aaaaaacgca taaaatcgtg ttgctaagaa cgttgatttt atgcgtttta ttatatgact
      961 tatctcgata tttttattga aatttatgtt tatgcccaac tatacttctc agaatcaatg
     1021 tccatatctc atctcagcct agtatagatt gacagattta ttctacattc ccagttcagc
     1081 ctctgcagct attgcgaatg gctcgtttta tattcaatct ccaaagattt cctaaaactt
     1141 ggatctgtac ggagggtaaa acaattcgat acactattat aggcataatc tctactctat
     1201 taaccatatg tatatacaag tagaaattac acgaccaaca tttactccga ttcttgctta
     1261 cgttttgtac gataaagtac ctgagctcct acaagaagag agagggcagt taacgtatag
     1321 attggagaat cagcttgtcc agtagatgga agggtatgtg atttctcgtt tgaacgattc
     1381 acgctagaaa ctttctccat attttcagaa agacgtgatt tctgattcat agcaagctct
     1441 acctgatttg ccaccgtttc ggcattcact ttatctttga tagtagaaat tggagcagag
     1501 ttatctgaat ctttaatagt cttagaagaa tctggagtat cactattttc tgctttatca
     1561 atctcttcag ttgactctgg tttcattggg cgagacaaat ctggggtatc cgttactttt
     1621 ggaattgttg agtctggttt atttgctgaa tcatcggttg agtcttcgat aggcttaagt
     1681 gattctggct caacattatt gcctggtttt ccggtctctt taggtgactc tggttgcatt
     1741 ggaagagttg cttctggaac atttggtttt tcctgcatag gtgtttcagg agtcccctta
     1801 ggcgtttcat tctctacttt tggcatatca attggcttgt caggaacttg tggctcaata
     1861 ggaacaggaa ccgaaacttt cttagtttta tggttaagag acacttttag ggatgagtat
     1921 actttcttcc caactttatc attaatctca tggctaatct ctgtacttac aagtttttgg
     1981 gaatctttct tctttttatc tttcagttgt aactgataag ttaaagaaag tggttgatta
     2041 gtctgataag ccttgattaa gtcttcttta tctaagttcc aagtaatagt tgttttcgag
     2101 ctagtaatcc atgacagcca gaacgttgaa gaattctttt taatacttac tttagggtct
     2161 ttgggttctt taacaatttc aacattatct tcaagagtat ctaccaatgt cactttagtc
     2221 agtacatctt taaatttttc tgcaacattc tcatcaacac ttaaaaactt cttatcatcc
     2281 ccagacattt ttttcaagta ttcatcaaat ttatttttat tcttttcttc gccagctttg
     2341 tctttggtca catcaacagc gtagacatga ataccatttt tagtagcaat cttggcctct
     2401 gcttgagcag agaggttgtg gttgttaaaa atattttttc taccgtagaa aatataatca
     2461 acgagggaat tcgcaaataa atcaattgct ttttcagaag tattcgttaa accgagcttt
     2521 tcagcgatat agctaagagt gtttgtaggt aactcagctt tttttaactc agttgttaaa
     2581 ttatgtttta tcttgctaac aatttgattt ctaaatgtca acggctcaac tttacgttca
     2641 taaaagctac ggaaatgata accttcacca atttgtttct cgtaattgaa atttttttct
     2701 tctaactgcg aactgtctac atcaaccaaa ttaatatagt ctaacggatc ttttgcaatt
     2761 attcctataa gctttgttat tcctgctata gtggatacat catctaaagc tttatcgaaa
     2821 cgtgtaatcc caactttgtt caaaaaactt attagactct ttgcatcatc caacaagttt
     2881 gccctgcgaa cagtcacatc aaatgtaaat ggccaaggca agagtggatt tgtatgtaca
     2941 accttatttt cctcaatggt cttataagta ggttttgatt ttacatcatc cgacaaatca
     3001 taactaaatg tagactcccc ttgtgacaga agtacaatat gtttgcgttt ccccgagttc
     3061 tcgctttcta aaatgcgacg tgcttcatgg attgctccct gcatatctac gccccaattg
     3121 taattttgtt cagcatcagt gaaaagcttc tcaaccgtag tttctacatt agcactggta
     3181 tggtaacttc ttgagcttca gataacttgc ggttgaaacc gattacacgc acagagttac
     3241 gagaattatg atcattggtt gggtcagtca tcttttcgac aagctttata atgttatctt
     3301 tagctgtttt ttagcatctt catccatttt tttatatgta tccaccaaga caactatttc
     3361 tgcacccttg tcaatttctt tcggcacaat ttgagttgtt actgtgacaa ctccctcttg
     3421 atctacttct gctgttcgag aaactgtaaa aagctcttta gaagttttgt ccactgtact
     3481 agatactgtt tccgttggtt tttcaacctt agtagaatag tcgtctactt ccttctcctc
     3541 atactgaacg gttcgttctt ctttttctgt tgctttttct tcagaagaac tagtgttttc
     3601 agcaacttgc acttctgatt ctgaagcgtt ttcattcgtc aatgcctcag gagaagcaga
     3661 ttctgcattc gattttggta agtccaaagt agaagaggag tcaaccacct ctccttcctt
     3721 gttttcatta gcttgggcaa cttcagggtc ttcaacgaca gcagtagacc taatcagaga
     3781 agtgctatct gtctccgaag ttttttctaa agtaggtgtt tctgcatata ctgtcacagt
     3841 atttttggga tagagtgttc ccgataaagc tacccctgct cctagcgaaa caagtccaat
     3901 tgatagcttc cgcaaagtga atttaggatg attcattatg ataccttctc cttgttctct
     3961 ataaatttag aatgtatttt cacccttctt taggtatttt caaaaaagga agtatggatg
     4021 tcaatggtaa ataactttag tataattttg ttcaacaagc gtagagcttt tggaaaaaga
     4081 atgggcacct tgtgtattca taaggtgcct aagtccttct gttaactgct tacttcacct
     4141 atcaagtcaa gcaagcatga ttacaactca aagttcctat ttatcattaa caatgaccat
     4201 atcacaaaat gtgcgagtct attactacat aactcattct accatataat ttaaaaacag
     4261 aatttgtttt ttctctatcg tttgtttatg tttattctat tacaaaaatg ataattggtc
     4321 tttgagtctg ttatcacaca catttataca agtgataaat attgcaatga gtcttcaaac
     4381 tctactttca cctctataag caaataattc attgaaagaa aaattttaac tcttttaatt
     4441 taaagctaca caatgcctct catctgtcgc aaacgaattt ttgagagagc tttgtcatcg
     4501 ccttttactc actttctgtt atctttttct tgccaatgaa taaagcaaat aagaataata
     4561 tgatcgataa cattatattg gcaaccaagc tcgttttttc accggtctta ggcaggcttt
     4621 gaacttgggt attctcacca ctatcatgca cagatacgtt tccttttgtc cctttgtctc
     4681 ttacagtacg agccattttc tcttgcttag taggcatatc ttttttcgaa tcatcaaaaa
     4741 tagaaaccgt ttcttctttt gatggctctg cattttgctc tacttttttg tcagtcagcg
     4801 tttggcgtac aggtggcaaa gtagtttgct caagtgtgtc tgattgttcg atatttttgg
     4861 taatttcttt gctaggttgc tcttccttct cgtccttttt atctggttta ttaatgcggt
     4921 gaccatttcc tttttgaacc gttacgactt cccaagctac ctgttcacct tctttatcaa
     4981 acgctctaaa cgataaggtt tctccatttt ttaaaacacg actacccaaa tcaaattcga
     5041 atcgacctgt tgaatcagtt gaagttttac caattatctc ctcgccattc tgagttgata
     5101 ctagttcaat cattacatct ggagctgttt tgccactgat gtaaccatga tctgtagtga
     5161 ctttttctac ttgaaatggt aaatccatca cgtcttcatc ttccaaatat tgccctacaa
     5221 cttcttcctt actagcgatg atattatttg taaccgggtc aatgatttca aagcgaattc
     5281 tctcaccttt ttcaaaaggc aattgtaaat cttgaatttg gaaaccaaaa aatcctaatt
     5341 cgtccacttg aactggagga tactgttttc ctttgattga cgaaaccatg cgaacgtagg
     5401 cattaagatg tatcgttcga ccgacaatat aatctttttt attatgcagt ttaggtacaa
     5461 tgataggatc ttcatctgtc gcttcatcca tatctgcatc ttgtaaataa gttgctaaag
     5521 atgccataac agatttatcc aataatatac ttgaaccatc aactgtatca agatggacag
     5581 ataccaagtc ctcttgttga ttgtgagtca atctgtagta cttatcctcg gattgtaggg
     5641 caggcaaagc aatcttatag tgacctgtct catcagcaat agcttccatg ataaactctg
     5701 aagtatcagc attgtataga tgcacacgac ctaaaggaaa tgtagttcct tccaaaactg
     5761 aagtcgcact tgataaaggt tttactgtca tttgtgaaga tactttttct tttaccaatg
     5821 gttttacaat agtattagta atcaccggct gaccatcttc tgatacaaaa gacaaaacca
     5881 atctatcatc tgctttaaat tttgaacctg ctaaactctc ggaaaaagta acctcaaacc
     5941 gtccatcttt aggatcaatt agtgtcttat cagagacaaa actaccattt attgatagat
     6001 agactgaacc tttgacagag gtatgaccaa taactttgct cgtatgttca aaaactggtt
     6061 cgatgaacac ttgatgcatt ccattagatg ttttttctaa tctttttgct ggaatttcat
     6121 aagcctctgg atgtcgatta gtcctcacta ctaccttacg ctcttcagaa tctgcctcta
     6181 gagtccaaaa ctgtttcggc aaagcactaa tttccacagt ttggctgtga gcaagtggtt
     6241 tactcaaccg ataagaaaaa tctcccttat catctgcttc caaaatattc tcaatagaag
     6301 taattgcttc cccatctatc gtaatcgcta catatccacc aggagtggta ttccctgaga
     6361 tggtttgatc tccagcttga atcggagcaa cctctagatg gttttcaata aatggaagtt
     6421 cttcctccat atcctccatc tcttccgatt ttttttcttc actcggtacc tcatcagtca
     6481 cttcctcagc ttcagcattt tccgaaactg tattggtaag cgatgattcc ttgctttcta
     6541 attgagtttc aagagctgta gcgtcagtct cctcagccat tgcggtttcc atattcatcc
     6601 ctgtaccaat ccatagaaaa gcaattgcca ccgaaactaa acccatctta ctttttctca
     6661 acgaaaaaat cttgctattc atcttttctc cttatatctg tattaataat taacgacaca
     6721 tattgtccta gttttattat aaaaaaaaca tctcatagaa cattgttttt cattagtttt
     6781 ttatctgttt tacagttgcc tgtaatatat agtatgctac atttcgcaaa aactttgctg
     6841 gttctcatcg tcatttagtt tttcttttat tacttctgta atcaatttga attgacagaa
     6901 cgattagaag cactttactt gtaaacaaaa tttctaaatt atggcacatt caaagtaaaa
     6961 aactagcgcc tgaagttttc tatgatacag atacttgttt atctcggagt gctcctttaa
     7021 ttcgtcaact tatactcaat gaaaatcaaa atcaaactag gcgacgcaga tacagataga
     7081 actgaagttc atcaaatcaa gttaacaacg tctgattttc gaagagtatt aaataagagt
     7141 gggtttattt gtcatgatgc cacttgttta ttttgttaac tatcacttca taaaacatga
     7201 ttgtcctcat aataaaaagc acccgaaggt gctagataag gccttcctac cgacttcacg
     7261 ttcgaggtcg gtagaaagct tcattaggcg ttgaaagcat ctgtcaattg tggcaccact
     7321 tgtttcttac gggaaacggc acctgcaagg aaagcgtggt tgttttccaa cttgaagttg
     7381 aaggccgctt ccaccttgtc catgttgctt cctagggcaa ggatttcaga gtttgagttg
     7441 acgatgtccg taatcatgag gacaaagtca gagtagccat ttgctgcgga agctgctgtc
     7501 atagtggctt caatctctgc ttggcgttcc aaaacttccg caatatcaac agtgttgacc
     7561 tgagctacac ggacgtcatt gccattcaaa ccaaatgttt tggcatcaat gtcaatcaat
     7621 tcttctgctg acttgctggc aaggtttgta ccagctttca agagtgtcaa accgtattct
     7681 tccaagttca caccagcaat ttcagccaat tcagctgcta cttgtggatc tgttgtatga
     7741 gtggttggcg atttgagcaa gagagtatct gaaatcaaac ctgataggag aagtccagct
     7801 acttcttttg gaggtgttac tccgttttct ttaaaggccc ggtaaacgat tgatgatgct
     7861 gaacctactg gttccaaacg catgtacaat ggatttgccg tttcaaagtt tgcgacacgg
     7921 tggtggtcaa taacagctgc cacttccact tccttgatgt ctgcgattga ttgttggaat
     7981 tcattgtggt ctgtcaagat gacttggcta acgccttctg cttttgcaga ctcaaccacg
     8041 cgcggtgcag taacaccaaa atagtcaagc gcaaacgctg tttcttcatt tggcgttcca
     8101 agtgcaactg cttccgcatc acgaccaaac acttcacgct ccaagtgagc ccagccgtac
     8161 gatgatgcaa tggcatctgt atcaggattt tggtgaccaa aaactaaaaa tttagacatg
     8221 tctacaacct catttttcta ataatctcct acattttacc ataaatagaa agacttgtga
     8281 acatggacgg gtcgggattt gccaaacgtt ttcaaaaaga gtaagataga aggtagcaat
     8341 acaaaaggag aaacatatga aattttcaga ctatacctat gtccgtccag attatgaaac
     8401 tatcaaggct caattttctg acctgacaga ccagttggct caggcaagtg acttggaaac
     8461 aactcgcaca cttgtggcag ccgttaccaa attgctcaac ttggttgata cccagtacaa
     8521 cctctggatg atccgtcata ctatcgacat gaacgatgag ttctacaatg aagaaaccaa
     8581 gttttggaat gaatattcgc cactttttga agaattgacc accaactact accgtgtcat
     8641 tgtccagaca ccttacaagg ctgagcttgc agacatttta ccagaaacct tcttcatgca
     8701 ggctgagaac aagctcaaga ccttctcatc tgatgttatc cccttgttcc aaaaggaaaa
     8761 tgaattgacc gatgagtaca gcaaactgat tgctggagca gagattgatt tccaaggtca
     8821 gacctacaac ctggcacaaa tgggaccatt cggtcaatca accgaccgcg aggtccgcaa
     8881 agctgcttct gctgccacaa ctgcattctt tgaaagcaaa gaagctgact ttgaccgtgt
     8941 ttacgatgaa ttggtcaagg tccgcacgga aatcgcccac aaacttggct tcaaggacta
     9001 tgtggaatac ggctacctca agatgaaccg tttcgattac aattgtgaca tggtcaaggt
     9061 ttaccgtgaa gaaatcctca agcatatcgt accgattgtg caaaatcttc gtgaacgcca
     9121 agccaaacgc ttgcaggtac caagcctgaa acactacgac ctcaatcttg agttcttaga
     9181 cggaaacgct gtgccacaag gtgacccaga ctttatcctc agccaggccc aagacatgta
     9241 ccgtgaattg tccgcagaaa caggcgagtt cttcgatttc atgattgagc atgaactctt
     9301 ggacttggtt gcaaaaccag gtaaaaacag cggtggctac tgtacctata ttcctgactt
     9361 caagagccca ttcatcttct ccaacttcaa cggaaccagc ggtgatattg atgtcttgac
     9421 ccacgaagca ggtcacgcct tccaagtcta tcgttcacgt tggattcaaa gtccagaagt
     9481 tgtttggcca acctacgaaa cctgtgaaat ccactccatg tctatggaat ttatgacgtg
     9541 gccatggatg gaccgtttct tcaaggaaca ggtggataag tacaagttta cccacttggc
     9601 aagtgccctt ctcttcttgc cttacggtgt cttggtggat cacttccagc acgaagtcta
     9661 tgaacatcca gagatgacac cagctgaacg caaggcgact tggaaaaaac tccaagacct
     9721 ctacttgcca gaccgtgatt attctgagtc agaagccctc aaccgcggca tcttctggta
     9781 tcgtcaaggt catatctttg ctagtccatt ctactacatc gactatacct tggctcaagt
     9841 ttgtgccctt caattctgga aacgtacaca ggtagatcac gatgaaaatg cttgggaaga
     9901 ctatatccgc atctgtgatt tgggcggcac caagtccttc ttgcaagtgg ttgaagccgc
     9961 aaacctccaa tcaccgttca aagaaggtgc ccttgaaagc acagccaagg ctgctgccga
    10021 ttggttaaat gcagttaagg atagcgactt ataaaacaca aaaatctgaa tgaatcaaat
    10081 gactcattca gattttttga ttatatgata cttgtcccta acacattaaa tctttttcgc
    10141 agcaatctca gacctctcac tattatccct taattctatt caagtactcc tgataggtct
    10201 ctgtctccat caaggccttt gcattttcca ctcgagcctt cgttgggggc ttggtcccct
    10261 ctagtggata gggtctgccc aactcacgcc acttaaattc accaagattg tgataaggta
    10321 acacctcaaa acgttcgaca ttttttaatg ttttaacaaa cttaccaagt tcaatcaagt
    10381 cctcatctcg gtcagtcaaa ttcggcacca ggacatgacg aatccaaaca ggcttaccaa
    10441 tgtctgatag atattgagca cattctaaaa tagtcttgtt actatgacct gtcacaatgc
    10501 ggtgttgctc aggattcatt tccttaatat caaggagcac caaatctgtt acttccataa
    10561 gccgactata tttttccaag taacgctctg tcttacggaa agtcaacgca caggtatcca
    10621 aagtagtgtg aatccctttt tcttttgcaa gggtaaacag agcaattaaa aaatcaatct
    10681 ggatggttgc ttcaccacct gaaacggtaa tacccccttc ttttccccaa aacatacgaa
    10741 aacgcagagc ttcattcaag acatcttctg ccgttctctc agtcgctgct ggattgacca
    10801 gatcccaagt atctgggtta tggcaatact ggcaacgcat gtgacagccc tgcataaaaa
    10861 ctacaaaacg tacccctggg ccatcaaccg aaccaaaact ctctgttgaa tgaaccaaac
    10921 ctgtcacttt acgataatca atttctgtca tcatctcatc ctcatcattt tataagacta
    10981 gtatatcaca aaaatgaaaa cgcttctatc atttactttc tttcataacc atcatcttcc
    11041 gcaagaaaaa agctaaaaga ctaggttgcc ctagtcctta tcggtttctt ctttctccag
    11101 tggtgtaatc aagagtttta attttgttac gcgaccattt tttaccttat cgttaataag
    11161 aactaggtgg tgatctttac tatctacttc aaaggaaact ttctcatctt ggctcggaat
    11221 ggtaccgact cctgtcagat aataaccagc aatcgtatca acgttatcac tttccaactc
    11281 catctcaaaa tgctcattga agtcattcaa ggtcatattt ccttggacga tataggtgtt
    11341 gtcagcaatt tctcgaacaa aaatttctgt cttatcggtc tcatcatcga tttccccaac
    11401 aatttcttcc agcaaatctt ccaaggtaac gagacctgca actccaccat actcatctaa
    11461 caagatggcc atttgatttt gggtatttct caatgatgtc aacaaatcat ccacgaaaat
    11521 cgtttctgga acgaagagag gctcctgcat aatgcggcgg atgttgagat tgtcaaagcc
    11581 attactgaaa gcttctgaca agattttctt ggtatgaatc agaccaatga tattgtcctt
    11641 atcgccatca taaacaggga tacgtgaaaa attctgcttg aggatagctg ccatgatggt
    11701 tgctgtatca tcctcaatat ccaccatgaa agcatccgta cggggaacca ttacttcacg
    11761 tgccatcagc tcatctaaag agaacacacc ttgaagcatt tcgatttctt cggcatctaa
    11821 ggttttctca ctctttgtca aaatatattc aatttcatcc cgtgtcattt gctcatccgc
    11881 atcatcaaaa ttcataggag taatacggct gataagattc gttgcagctg ataataacca
    11941 cacgaacgga ctaacaattt ttccaaggaa aataatcact ggtgcagtga ctactgccag
    12001 attttccttc atattcatgg caatccgctt agggtacaat tcgccaagaa cgattgaaac
    12061 gaaggtcaac atagctagtg caagccagta tcctgcggtt tgcgctgttg ttgaattccc
    12121 catccatttg gcaaatacag ctcccaaatc attagccaag ctggcacctg aaaggatgga
    12181 gatgaaggta ataccgacct gaatggttga tagaaaattg ttaggattct ctaaaacttg
    12241 aagcaagcga atatatttcc tctcaccgtc tgcagccttt tgttctactc gagaacgatt
    12301 gagggataca agggccattt ctgaggctga aaagaaggca ttgaacagag tcaataagaa
    12361 taaaattaac agttgtaaat agatggtctg actaccaggg tcttccatga tgttctccta
    12421 taataaaatt tataacattt ccattatacc acaaattcta aaatttaaca tatttcttgc
    12481 tataattagt ccctcatgtc tgtttatgtt acaataatca aaaggaatag gagagcaact
    12541 atgattattt cgatgaagaa tgtccactat aaacgacaag gtaaaactat gttagaagac
    12601 atcaactggg aatttaaaaa aggcgagcgt tgggctattt taggactcaa cggggctgga
    12661 aagtcaactc tacttcgcat tttaatggct gaattttgga agacctctgg tgacctgact
    12721 gttcttggag tggagtttgg taagggagat attcccagcc tacgcaccaa gattggggta
    12781 gttggctcct ttttggcaga gcgttttcca atcgatttga cggctgagca gattgtcttg
    12841 acaggaaaat ataaatcctc catcctctat cgtgaatacg gtcaagctga attggatgaa
    12901 gccatagaca tgctcaaaac cattaaggct gagcacttga tcggtcgggc ctatgctagt
    12961 ttgtcacaag gagaacgcca actcctgctg attgcccgaa gtttgatgga agagccggct
    13021 attttgattt tagacgaagc aactgtcggt ttggacctcc tagctcgcga gcgcctgctc
    13081 caccacattg actacctctg tcaactccca acggctcctg ccattatcta cgtcacccat
    13141 cacgccgagg aaatcactga taatttcacc catgtcctcc tgctcaaaga tggcatggtc
    13201 ttggcacagg gacccaaaga agatatcctg acacctgaac tcctcagcga tttttatgga
    13261 aatcaggtgg aattgattga cttgggtgag ggaagattgt tcattaagcc gatattataa
    13321 aaaacaaatc cagctctagt tcgagctgga ttttaatcgt tccaccatga ccaaaaatgg
    13381 tggggtatta acttggttga gcggtttgta gagcatggct gtaaagacgg tttggtcaag
    13441 ctggctgata aagtctaaaa ctgcatcctt ctcaagagct ccaccctcgt gaccatagta
    13501 aatcatgata gccaagcgac ctccaaccac caatctattc aggactttct caatggcttg
    13561 aagggtggtt gcaggcaggg taatgactga cttgtccgcc gaaggtagat agcccagatt
    13621 gaaaatcgct gccttgcatt catccacata ctggtctaaa ttttcgtgtc ctgtcaaaac
    13681 caattgggca ttggttaagc cagctttttc tagtttttca gccgttgtgg tcagggcttg
    13741 ttcctgaata tcaaaggcca caacttttcc tgctcgttgg gctaaaaata gcgtgtcatg
    13801 gccatttccc atagtcgcat cgaccgctag gtcctggtca gttaaaatct catctaaaaa
    13861 ggcatgggcc atgtgtaaag gtctaagcat actttctcct aattactgta ctttctacta
    13921 ttttgtaaaa tttatcacac ctagtatagc atactttcca tcttcaaaaa attttttaaa
    13981 atctactcat attcctccat ccatttacga ataaataggt atgaagacaa ttatcctatt
    14041 tttccttgga gcttctatcg gctccttctt gggattggtc atcgaccgtt tccctgaaca
    14101 gtccattatc gccccctcta gtcactgcaa tgcctgcaaa cgacggctca aggcatggga
    14161 cttaattcca gtcctatccc agctttcgac aaaatccaaa tgccgttact gcaaggcgaa
    14221 gataccttat tggtatctgg gactagaatt cttagctggt ctagttgtcc tgctctgcca
    14281 ttttcaagtc ctaaacctaa ccgaaaccat tctcatcttg gcaggactag ttttgaccat
    14341 ttacgacatc aaacatcagg aatatccttt tgctgtctgg ctcattttta cttttattgc
    14401 tctgatactc tcccagctca actggctctt ctgtggcttt ttactcttgg cctatctgac
    14461 tgaaaaatgg caaatcaata ttggttctgg tgactttctc tatctggcaa gtttggcctt
    14521 aatatgtgga tttacagaac tcctctggat tatccagatt agttccctcc tagggcttct
    14581 tgtcttcgcc attttcaaac ccaagtctat tccctacgta ccactccttt ttctttcaag
    14641 tattcctatc attctgtgca tctaaatcga gtttttctcg atttttttgt tataatagaa
    14701 ggcatattag agttcaactc catcaaaata attggagtgc cttcttgatc ggaaggcaaa
    14761 aggagaaacg ttatgacact atttaaggat gacagtttaa ccctgcatac cgacctttat
    14821 caaatcaaca tgatgcaggt ctattttgac caaaacatcc acaataaaca cgctgtattc
    14881 gaagtctatt tccgttcaca accttttaaa aatggctacg ctgtatttgc cggtttggaa
    14941 cgcgttgtaa actacctcaa caatctcagc tttagccaga cagacattga ctatctgaca
    15001 gaactaggtt atccgcagga tttccttgat tacttgtccc aattggaaat gactctcact
    15061 gtccgttcgg ctaaggaggg cgacctagtc tacgctaacg aacctattct tcaaatcgaa
    15121 ggaccattgg cacaatgcca actaatcgaa actgccctct taaacatcgt caactaccaa
    15181 accctgattg ccacaaaagc tcgccgtatc aaggctgtca ttgaagatga gcccttgatg
    15241 gaatttggca ctcgccgtgc ccaagaaatg gatgctgcta tctgggggac tcgagcagcc
    15301 tttattggtg gagcatctgc gacttccaat gttcgagctg gcaaaatctt cggcatccct
    15361 gtcgctggta cccatgccca cgccttggtt caaacatacg gagatgatta caaggctttc
    15421 aaagcctacg ctgaaaccca tcataactgt gtcttcctcg ttgataccta tgatacgctt
    15481 cgtattggtg ttccagcagc catcaaggtt gccaaagaaa tgggagataa gattaacttc
    15541 aaaggggttc ggattgattc aggcgatatg gcctatattt ccaagaaagt tcgtcagcaa
    15601 ttagatgatg caggttttac agatgcgaag atttacgcat ctaatgacct ggatgaaaat
    15661 accatcctca acctcaaaat gcaaaaggca aaaattgatg tctggggagt tggtaccaaa
    15721 ctcatcaccg cctacgacca accagctctg ggcgctgtct acaagattgt atccatcgaa
    15781 gatgaagatg gaaatatgcg tgacaccatc aagctgtctt ctaatgcgga aaaagtctcc
    15841 actcctggta agaaacaggt ttggcgcatc acctccaaag aaaaaggaaa atcagaaggg
    15901 gactacatca cctttgcgga cactgacgtc acacagatgg ataatatcta tatgttccat
    15961 ccaacttaca cctatatcaa taaaaccatc gagaactttg atgcccgtcc tctcctagtt
    16021 cctattttcg aaaaaggaaa acaggtctat gaacttccaa gcctagcgga aattcaaacc
    16081 tatgccaatc aagaatttga taaactctgg gatgaataca aacgcgtgct caacccacag
    16141 ctctatccag tcgacttggc acaagatgtt tgggataata agatgaacct catcaatcgt
    16201 attcgcaaac aaactcagac caaatccatc taagaaaggt atgttatgac cctacaggaa
    16261 acaatcatca aagaattagg cgtcaagcca agcattgacc ccaaagaaga aatccgtcgc
    16321 tccatcgact ttctcaaaga ctatctgaaa aaacacccat ttttgaaaac ctacgtcttg
    16381 gggatttctg gtggacaaga ttcgaccttg gctggtcgtt tggcccagct gactatggaa
    16441 gaaatgcggg cggaaacagg tgacgattct tatcaattta tcgctatccg cctgccttac
    16501 ggtgtgcaag ctgatgagtc tgacgcccaa gcagccctag cctttattca accagatgtc
    16561 agcttaacca tcaacatcaa ggaatcaaca gatgccatgg tggctgcagt caatgcaaat
    16621 gggcttgcta tgtctgattt taataaggga aatgctaaag cccgcatgcg catgattgcc
    16681 caatacgcta tcgctggaga acgcaagggg gctgttattg gaaccgatca tgccgctgaa
    16741 aatatcactg gtttctttac caagcacggt gatggaggtg cagatatcct gcccctcttc
    16801 cgccttaaca agcgtcaagg taagcaactt ctagctgctt tgggagcaga tgaaaaactc
    16861 tacctcaaag ttcctactgc agacttggaa gaagacaaac caggccttgc tgacgaagtc
    16921 gcacttggtg ttacctataa ccaaatcgac gactatctcg aaggtaaaac catcgaccct
    16981 caatcccaaa ccattatcga aggatggtgg aacaaaacag cccataaacg ccacctgcca
    17041 attactattt ttgatgattt ttggaaataa tactcctcga aaatcaaaat cagacgttat
    17101 tgacttgatt tgatgactga aaagctcctg tggaggtttt cagcctgttc ccttgaaata
    17161 taaaaggaac agagttctaa catcaacttg aaggcgcaaa caatgttcgc tatatttcca
    17221 accttcaact atctcccaga tagttgaagc taggctactt ttgattttca tcgaataata
    17281 aaaaatggag ccctgacagt tgaaccgtca ggggttttgt tttttattat aacttcaaac
    17341 cataatgaaa aaagcccacg caggaaaaaa tatcctgcgt gggcgtcaat ggacacggtg
    17401 ccacctcact atggactcga ctagaaaagt ccataacttt ttctcctcta acaaggaggc
    17461 gcacgataag gggtgcggac cgaactctca ttgtttcgaa ttcatcgaac caaactcact
    17521 ttactaaacc agacaatcca taaaatggat acgaatgctt gtttggcatc agcctatctc
    17581 ataaacctct atcatctatt cacaccaacc atagactctc taaagacaaa gtgtttatta
    17641 cattttctga ttacttgtaa ttatacgtta caaaatcaaa atggtcaagt gatttttaga
    17701 aattttctaa acaatttgaa aattcagttt ccattgcctg aatccattaa aaactaacaa
    17761 aaagccactc tgcttaagtg actttctaaa aaactgtatt cacgagcgaa gcactcatat
    17821 caaagatagc tttagctagt caaggtcatt tttaagggaa tactgactgt atttcaaaaa
    17881 aataacaatg tgtagcataa acaatagcct tagatggaca ttctgaacct gtgacggtag
    17941 gttctaagac tcctgtttgg aaaggattat ccctctcatt atcaatgaat aggtaaaata
    18001 ggctagtatt ccccccattg tattggtcca taaatcatcg atttcaaaga cgcgttgggc
    18061 atcaatcagc aaatctagta gtaattgtgt gcattcaata aaaagactga tacaaaaact
    18121 ataacgaatc accgctcgaa ggctccgcca ttcttcccta agaaacaaaa gaagaaaaat
    18181 cagagggaaa agtagaaaaa tattagtgat gttttgcaag aaaatccacc ctaggtccgc
    18241 caaactatct accttgtccc cgttgacgat gctgttgaaa ggagttggta aaagatagag
    18301 acggccaatt tgaataattc ccggggtcga gaaatcctta tactgtgggt accaagtctg
    18361 aggtagaaaa caaagtaaaa caatagccag agcataggct ccagctaagg tcttacaaat
    18421 ctgtcttcct agcatggtca agtttccatc tgcttggaag aattttctca tgctcgaata
    18481 cctgcctttg cttttgcggc tgcaatacga gcacgagctt tttcacggtc tttcttgagc
    18541 gtattggaac gcaattgacc acaggcagca tcaatatctg taccatgttc ctgacgaacg
    18601 acacagttaa caccattttt cttcaaaaca tcaaagaaag caagagtacg ttccttggtt
    18661 gaacgactgt actggtcatg ttcactaact gggttataag gaatcaagtt gatataagac
    18721 aatttacgaa tgttcttggt caaatcagct agttcctgcg cctgttccac tccatcgttg
    18781 acctcattga gcataatata ctcaaaagtc acacggcggt tggtcacttt gatatagtct
    18841 tcgattgctt caaaaagaac ttctataggg aatttgcggt taatgcgcat aatgctcgaa
    18901 cgaaggtcat tatttggcgc atgtagagat actgccaagt taacttggac accttcacat
    18961 gcaaactcac gaattttcgg tgctaaacct gacgtagaaa cagtgatgtg acgggcacca
    19021 attgccaagc ccttgtcgtc attaaccact cgcaagaagg tcataacatt atcgtagtta
    19081 tcaagcggct caccaatccc catcacaacg atatggctaa cacgctcatt ctgattgcgc
    19141 tcatccaagt acttttgtac caacataatc tgagccacaa tctcaccgct agtcaagtca
    19201 cgttgcttag gaatcaatcc agatgcacag aatgtacagc cgatattaca gccaacttgt
    19261 gtcgttacac agactgaaag gccatagtgc tggtgcatga gaactgtttc aatcaacatg
    19321 ccatctggca gttcaaaaag gtatttgata gtaccgtcct tagactcctg tacaatccgt
    19381 tgtttgagcg gattaactac aaaatgttct tccaactttt caatcaaaga ttttggcaag
    19441 ttggtcattt ccgcaaaaga ttggacacga gaacggtaaa gccattccca aatttgagtg
    19501 gcacggaact tcttctctcc gttttctaaa atccaatcaa ccagattggc ttgactaaat
    19561 gcataaattg atggtttcat atttctccct atttctggcg gataacaaag ttacgcttca
    19621 cttcattcat ctcacgctta ctctgcttac gctcatgttt ttgagaacgc tccgtttgac
    19681 tcgccttaac accagcattc ttcatagtaa aatgacgctg ttccgactgt ttattttgct
    19741 gcttcccttt tttctgacgg tcattgcgat tacttttgtt ttcttttggc tcaataagct
    19801 ccgtccgttg ctgatttctg ttccgattgc gagaacgact ccgcttccga cgcggtctat
    19861 cctcctcctc agcaggcaag tcttgtggat tcggattggc taagacgaaa taagcacaac
    19921 caaaattaca atactccgtc aaatagtcgt ccaagcggct gatttttaag tctgatttaa
    19981 catttttatc gtctgtatag aatcccctta atctcagctg ctcattgccc caatctccca
    20041 cgatataatc aaatttgagc atgagttgtg aaaaacgttg accaaaaatc gtctggtcaa
    20101 aagcattctt gtaatcttcc aaaaggtcca attcaatatt ttcagataca accttatctc
    20161 cgacacgagc aaaactcgga ccagggtatt tattatagtt atacatttca ggtgaaattt
    20221 cttttttcat ttgtcctcct atcttataaa atcttcatac gtaaaaatca tttgattaat
    20281 aatctatctg gtcagaatct aaattctcac acttaacctt atcattataa cacgatttat
    20341 ctggaaattc ttctacttta ccatttagga gaaatttctg acaattataa tattattctc
    20401 tagcaaaaaa ggacgattta ctcgcccttc gttttcttca aataatcaat agcctcttga
    20461 acagttttca caggaactac ctcaatatct agacctgctt tctcggctgc ttccttagct
    20521 tcttcatagt tcgttttagc ctttgggttt ttcttcaaaa cttctacatc aactggattg
    20581 tttggtacaa aaaaaatact tgcacctgac ttagctgccg aaataacctt cttatcagcc
    20641 ccaccaatgt cacctacttt tccatcctgc tcgatagtcc ccgtaccagc aataattcga
    20701 ccatcacgta agtcaggctc tgcgagttga gtatagatgg caagtgtaaa catcagacca
    20761 gcacttggac caccaatatc tcccgtttgg aaatcaattg gaaccgaact ttgaacctct
    20821 gtatggtcta cgagagtgat gcctattccg tttttgccat tagataattt gataatctta
    20881 ccgtccgcag atttttcctg accagcgcta atataaccaa ctgttacatc acttccaacc
    20941 tccaagccac tcacatattt aaccaaatca ggagaagact caaatgtttt tccattgata
    21001 ctcacaacag tgtcggcaat attaagaact tttttaaagg ttgaatcatc cgtcaaggca
    21061 agcacataga caccaaagaa atcaagattg acttcttttc ctgccaaggt caatccttga
    21121 tacttcgcca tattttgcga agtttccatg taaaaccgtg ctatacggaa atactcctca
    21181 ttgtcggccc ctcctgtcat ctcttcagag gaacgaactg tcgtataggg atcaaactga
    21241 gcagcgaata gttgtaaagc tgtagcttgt tggactgata cataggtgaa gttgtaagag
    21301 ccatcctctt ggtctatttg attgttcacc gttagtacct gacgaatatc ggcagctcca
    21361 cctggacttt ccagataata aggcagacga acaaaaacgg caaaccaaat caaaagagtt
    21421 cctaaaacaa tagatgcaat taaggttaac ttcctatttt tcttcattct ttctaatctc
    21481 ctcactaaca atttctggaa cataaggacc tatgtcctgt ttgaaataca gcaattctcg
    21541 aacctggctg gatgatacga acttaagctc tggcttggcg attaaataaa tagtttccaa
    21601 atctggcgcc aactgacgat tgtaaaaatc aaaacttgac tcgtattcca aatcggtcgc
    21661 attacgcaat cctcggacca aatgtgttac tccatagcgc ttagctacat cgactaccaa
    21721 ttcattttca gacagaacga cctcgatatt ctccattcct acaaaaagct tctccaaaat
    21781 ggcttttctc tccaaacctg tcaagagtcc tgccttttta ggattggtaa aaactcccac
    21841 atataattta tcaaatagtc cactcgctcg ttcaattaag tccaagtgtc cattggtaat
    21901 ggggtcaaat gagcccgtaa acaatccaat cttatctgac atatactgtt accttactaa
    21961 ttccgtagag tttctctttc catatcccca gctctgctat ttcctccgga agagagaccg
    22021 ccttatccgt ttcgcagact accataacat catctgctag taagtcacgc cgacaaagtt
    22081 ctgttatcac atcttcaatt tcctgctctg cataaggagg gtccaagaat accaggtcga
    22141 aaggacctcc cacactgttc aaagcctgtc tagcttccat tgctagaagc tgaaatttat
    22201 ctgcttcctt agtcattgca atattttcac ggataatggc ttgtgctcgt cgatctcgct
    22261 caaccagaac tgctgactcc atgccacgag atatagcctc gatagataaa ccaccactgc
    22321 cggcgtacaa gtcaagaact ctaccaccct caaaataggg accaatcata ttgaacatgg
    22381 ctccacggac cttatccgat gtcggtctgg ttgtttttcc atctaacgtt ttcagtggtc
    22441 ttccaccata gcttcccgat acaattctca taaggtatat tatagcaaaa aatgaagctc
    22501 gttgctgaaa ccaagtgcac ttcctgcttt cctttttgct ccaacttttt caaccttctg
    22561 ccaaatacac tctcaaccgc tatagcagga aatcattctc gcactattca acaaaatggt
    22621 aaaaataaaa agcaaggaaa ttctgtttta cgcgaaattc ctcactctta tcgtatgtgt
    22681 atttaaatta gagcgttaca agaaaaaatt ggtgttgaaa tacaaatagc tattttttcc
    22741 agtatgctaa tacaccataa agaaccagta gctaaaaagt ctacggatta tctggtgaac
    22801 tctcgtcagc tgttggaact gtttctactg actcaaccag gctagttgtt gaatccgtcg
    22861 tttccgccgg tgtttcagaa actgttggag tttctaccac aggtgctcct tcactagtcg
    22921 gagggactga agtacttgta ctagttgacg ccacgctgct tgtatactgg cttgttgaac
    22981 tcacactttg atttccatat atatttttat taggcactac ataggattct tgtgaggtct
    23041 cactagcttc agcaaggtcc gttgactgac tagtccctgt ttcagatact tttttctcat
    23101 cagctacttt tttggctgct tccgccctga ccaactccgc ttttagctta gcctgtgttt
    23161 gagcatctgt ctgctgtccc actagctctt gtacttggtc aatccgtttc tgtaatgctg
    23221 cttttttagt cgcatccgta accttgttta ctgcagattg cgcctctgca agtttctctt
    23281 cagtatgtgt tccttccaat acaatgacag cattctcagc atcctgaaat actgtatcaa
    23341 ccgttttttt ctgattgggc acttgctgta ctgtctttgt atcacttgtt tcgctttgag
    23401 catttttata ccaactagcg cctaagctaa tcaataaaat aagtccaata aaagaaataa
    23461 tgccaatttc tgccaatgac aacttttcaa atttcttttc catatttttg cccttatccg
    23521 tcttattgca taagaaaata ctctagccta ctgacaatgc tacttagtcg tttttacctt
    23581 accattccat ccgtttagac catagcttaa aatatagatt tcgttaaagc cctgtttctt
    23641 cagatgaagg gctgcaggcg ttaccaactg accacgatca ttctcataga ttaagacagg
    23701 tctgtcctta cgcaaagcag ctgtagactg gcgtaattgc tggtaaggaa tatttctcgc
    23761 acctaagata tgcttacgat taaactcact cgggtctcgc aggtcaatca actgacctcc
    23821 gtgtatcttt tctgcaaatt ctgcattatc aatcaccgtc gccgcacggc gcaatctcca
    23881 gtaattaaaa cccatccagg ctcccaatac tgccaaaaat actaaaaata cccaaaatgt
    23941 ttccatcact cattctccct tgatttctga tactctaatt ccaaacgatg ttcacgcctc
    24001 aaaacaagct ctgcttccat atattccaac cgatctaaga cacctgcctg atagattcta
    24061 tttaattcta tctgcatcat ttcaatatcg tagagacggt tgcccatata gacaattata
    24121 ccaaaccgtt tgagtaattg ttgcacatca taaagtcttt tcataaaaaa attgtagcag
    24181 aattttaggt gtttttcaat ataaaaaatc agttgaaatt tatgaaatgc ttaaaaagga
    24241 ttggataaat caccaatcct ttacaatttc ataaccaacc cttttcctgt gcaatcttaa
    24301 cagcagccgt gcggttttca gctcccagct tggccaagat agaggtcatg tagttacgga
    24361 ccgtcccgtc agacagatag agctggctgg cgatttcctt attggacaat ccattcgcca
    24421 ccaatttaag taggtgacat tcttgttccg tcaagggact gcgttgggtc aggagaatgt
    24481 ccatcaactc tggcgagtat tcctttcgcc ctgccaagac cgtttcaatg gtccgcataa
    24541 ggtccgaaat ggagcgttct ttgagcacgt aggcatccac atcagccttg accgcccgtt
    24601 caaaatagcc tggtcgcttg aaggtggtca tgatcactac cttgacgggc agggatttct
    24661 cacgcaccca ttccaggaca tccagccccg tcttctcagg catttcaata tctaagagta
    24721 gcacatctac tggctctttt tccaaaagaa caatggcctg actgccgttc tctgcctgta
    24781 aaacagcctc aaccgcatct tgaaagccca agagctgaca gagggcatcc cgcaacatgg
    24841 cctggtcttc tgcgactaag attctcattc taccacctcc tcaagcggaa ttcgtatgga
    24901 caggcgggtg ggttgtgcca gagaaagaaa ttccaaactc ccctgcaccg tcaccagtct
    24961 atcctttata gtatgcaatt cttgaccggt taactgggca aagcccacac cattgtcttc
    25021 gtagtgcaag actaattctt gctggtcttc ttcaaatatc aaacgacact tactagcccg
    25081 actatgtttg agcaggttat tggccaactc tctcagcacc atggccagtt tgttctggac
    25141 aggcaagctg agctgctctg ctatttcccc aaccaaaaca accaaatcca ctcctgctaa
    25201 atccaacatc tgctgcaaaa tctggagttc ctctgccacg gtatgctgct tgagggactg
    25261 gacaatctgg cggacgtcct gcatggagtc cttggctaag gtctgaagtt ctgccacctc
    25321 cttcttggcc tggtcaatct ggtcgtgatc taacaaagtc tggaccagct cggccttgac
    25381 agacagcatg gcaaagacat gccccagggt gtcgtgcaaa tcctgaccga tacggttgcg
    25441 ttcattctca gccagcagga gattgatgga agcattctgc tcctgtaggc gttgctgaag
    25501 ggcttcccgc ttggtttcaa tccaactgaa aatcagcaag gccaagccga aaaagtggat
    25561 aacaagtgca aaggctctca tctcaaaagg aacagggcca aagagagccc ataggataat
    25621 agccagcaac aaaagcccgt aggacaccgt ccgataggtc ggtcgattct ctttataata
    25681 ccagcccaac atattggaca ggttaaagat gaagagggaa aattgcagat tgccccaaaa
    25741 actcatgacg gcgatatagc caatcaggta aaaccaagcc agcatggaat agagcttgtg
    25801 gtcggtataa aagagggagc aataaaccaa ggcaaagaga atagtcgcta ggacaactgc
    25861 cgtcggattg ggagtatact gggagtaata aagtggaaaa tagagaaaga tcatgcccac
    25921 gaagaacatg agcggtacct ttcttttttc aaaaatcata gttcacctca ctattgctca
    25981 gtttttctat tcataactag agcaattcct aagaaaatta tggcatatcc taggacaatt
    26041 agcaaggatt tccagagaaa atctccctct tgagcataag tggtcaccag ctggttgaca
    26101 tggtagctgg gcaggagttt acagatccgc tgcacccagt ctggaaagag ggaaacaggc
    26161 atccaagagc cacccattat agcaaggaca aaatagagta gattggccac caccgacatg
    26221 gtttgttccg actgaatttg caccaaaagc aagccaatgg ctagaaatac gatagaggtt
    26281 actaaaagta agagagccga aattacccat tcttgagctg tcatctctac atccttaact
    26341 aagccaccca ccgcaaagac aatgataatg gaagatacaa agcagagcaa gacccgcacg
    26401 agtttggaaa gatagtattg ccagattggc aggggcgaat gctggataaa ggtcaaccag
    26461 ctattctttc tgtcctctgc cagcatcatg ggaaaggtga agagggcaaa gcccgacatg
    26521 gaaaagcctg tcatggtcag catgtaggac tggataaagg ccttttgcat ggctgcatca
    26581 tcaaactgga cggttgatga aaagatcagg aaaaagatga cgggcatacc gatagacagg
    26641 agaaatactc ccaaacttct ggataatttt accgcctcta cttgaactaa tgctttcatg
    26701 attcttcctc ccttgtcttg tcaaataggc tgttgagcag agtcttattc tgcacttcca
    26761 ggtctgaaat ccgacagcct gcctcctgca agctagccca aacctgctca atggctcggg
    26821 tcttgaagct gaccgtgtct tgtttatagc tgatctcatt gatttcagcc aagctctcca
    26881 ccaccgccgc aaaggcaatc ggcaaagtca cttctttttc ttgctcctca ctccgcatag
    26941 caaatggcgt ggtatctctt agtagccgcc cctggtgcaa gaccaaaatc cgctccgccg
    27001 tatgctccac ttcctcgata tagtggctgg tataaaaaat agtcacaccc tgatccttga
    27061 gttcatggac aatctcccaa aaacgctggc gggtcgaagt atccatgccc gccgttggct
    27121 cgtccaagaa gagcaacttg ggcttgccaa tcaggcagat gacgaaggcc aagagccgct
    27181 tctgaccacc cgatagcttg ccggccagtt gatttttttg ctctggggaa aagcagagca
    27241 ggctgtcgat ttccacatct gtcagactat ccttgtagat ggcttggaaa aagcggagca
    27301 attccttgac cttcaggtct gtaggaatgg cattttcttg tggtaagact gctacctttt
    27361 ccttattcga ctgagcctgt ggagccagac catccaacaa aacctggcct ttcgtcgcct
    27421 tcctatcccc caacaaacaa gacatgagag tcgtttttcc agccccgttt ggaccgatta
    27481 aagccacaca ttctcctgac ttgatttcaa aggaaatatt ggacaaaatc gtccgacctt
    27541 tgatgacctt gtctagtgcc cgtacttcta tcatttttct acctcctact tcttgaacat
    27601 cttatagtct tttagtttac ttttagtact aggcaacgag ccgcaagcat aactggagtt
    27661 aggcaaggcg agttaacgaa gtaataaaag aaaaactaaa tgacgataca tagacagtat
    27721 atctcgaaaa gggaagcccc actagaagcc tttgtcattg actcatgtga caaatgtcat
    27781 attatactca atgaaaatca aaagtagcct aggaaacgaa atcgaagata gatctggagt
    27841 tcatcaaggc aagttgacaa cggataattt tgatattcga agagtattaa ataatgcagc
    27901 aaaaaagagt cctactgggg acccttcatc tatttacaac ttaggcgctt gaccaagttc
    27961 tgcctgcagc atccaaatat gtttttcaat actagcctta gccacattga aaatgtcgtt
    28021 ggttacgctg tcaccttctt catcactgac atcaaatccc ttttggaaaa gagcagccag
    28081 atagcggaac acctctacca cacgcgccaa ttgctcttca atcgttacag tgtaatcacc
    28141 aggaacttcc ttgagctgac tattttcact aaactcttta agggtagaaa atggtgcccc
    28201 acctaaggtg attaaacgct cactcatctc atccaaatag ccatcaattt cttccatata
    28261 ttcatccatc tttggatgcc aaatcataaa gccacgacca cgcatatacc agtgaacttg
    28321 atggaggatt aaatgggcta ctgataaatc ggccaccgct tgattcaaaa cagccttaga
    28381 atcagccagt gatggacgcg ggctgaaaga tgcaatttct gctggagatt gataatattt
    28441 ttgtttcatc atatatcacc ctttctttta tttataatta ttataaataa aagaagaaaa
    28501 aattgcaaga attatccctc atcctcctct ggcttattga caaac
//
LOCUS       DAZQCJ010000017        28276 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000017 DAZQCJ010000000
VERSION     DAZQCJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28276
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.017"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..785)
                     /locus_tag="ACHU66_000673"
     CDS             complement(<1..785)
                     /locus_tag="ACHU66_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFR3388976.1"
                     /translation="MLRKVKNIFKKPMTLVTIIGIACVPALYNISFLTSMWDPYGRLD
                     QLPVAVINQDQSASFQDKTLTIGDDMVDNMKESKSLDFHFVSEKDAEKGLEEGDYYMV
                     ITLPEDLSEKATSLLTNQPEPITISYQTSKGHSFVASKMGESAMEKLKTSVSETITKT
                     YTTAVFDSMREIQTGMVEAADGSQQLTDGASQLESGSQTLSNGLTTLTTSGQSPVTGA
                     NQLATGVVSYTDGVNQAAIGSQTLSSGLTTYTNGVASLASGAE"
     gene            911..1444
                     /locus_tag="ACHU66_000674"
     CDS             911..1444
                     /locus_tag="ACHU66_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HFR3388977.1"
                     /translation="MGTDSRKERTRQAILEAMVTCLESQGFNDITTTHLAQTAGISRS
                     SFYTHYKDKYELIDSYQQGLFHKLEAIFDRYDGNRQSSFLEIFELLYREKLLSALLTH
                     NGTQEIQNFLINKVRLLVANDLAERLGKEDLSPLEKEYSSIYFSHAFFGIMQVWIKNG
                     KQESPQYMTDFLIKMLP"
     gene            1529..2512
                     /locus_tag="ACHU66_000675"
     CDS             1529..2512
                     /locus_tag="ACHU66_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR3388978.1"
                     /translation="MIQAEHLSKTYSIIDKEVGLKGSIKAFFKPKKKSIPAVQDISLQ
                     VGKGEIIGYIGSNGSGKSTTIKMLTGVLYPDQGQVRINGLNPQENRKAVNKQIGVLFG
                     QKSHLDWNLPVQESFILHAKIYDVPDKVFKERLAVLIELLDLADIMKQPIRNLSLGQR
                     VRCEFAAIFIHQPAVVFLDEPTIGLDASVKETIRSFIRYMNQEYQTTFLITSHDMKDI
                     ESLCERIFIIDKGKKVYDGSLTVLKERFSTVKTILFSTEKPIEKDLQLEGWEFIRKDD
                     FHFEIHYQNKVWTSAQVIEQVFNHYAIEDVTMKELEIETMLRQIYEEGIHA"
     gene            2505..3284
                     /locus_tag="ACHU66_000676"
     CDS             2505..3284
                     /locus_tag="ACHU66_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR3388979.1"
                     /translation="MRKYLYMTRLTMMNAMQYKAFFLATFVSLAVKILVALYVWKTIF
                     FTQSEVNGFTLQTFTTYIIFANLLASLNSFSLGEDLSYSILKGSIAGEFLRPYSFILA
                     LFFKDLGNKLLELIKFAIVFIGILLVHQDFYLPDGKTILLFLVSSILGMFIVQLLDMA
                     FGFLAFFTVNAWGVMLLRMGLFNLASGALLPLSFYPQAVENFLKLLPYNYAVNVPVSI
                     LLGQESDLTALGLQVIWIPILACFIALLWSQAKRRIVIFGG"
     gene            3286..4068
                     /locus_tag="ACHU66_000677"
     CDS             3286..4068
                     /locus_tag="ACHU66_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HFR3388980.1"
                     /translation="MKKYISLYLYNIKVYMMAQMSFRVDFFIGLFSSLIEQIVYLIFL
                     NILFGNIKEIAGFNYGQMLFIYGIATVGRSIHLIFFDNLWMFGSRYIRQGEFERLLLM
                     PVNPLFQLICEQIQPQGIGTTLIGSLALVQASKELGMEWSLGKLALLIFIAICIGLLY
                     AAIQLGPTALAFWIVESFPLTMGIFALNQMAQYPLNIYPKIIQILLIFVFPYAFTAYF
                     PALYFLDLSMWGLALPLVVVVLFSINYNLFRYGMTKFTSVGN"
     gene            complement(4118..4945)
                     /locus_tag="ACHU66_000678"
     CDS             complement(4118..4945)
                     /locus_tag="ACHU66_000678"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFR3388981.1"
                     /translation="MIVRLIATDMDGTFLDGQGNFDRERFSRVLDQLDQKKIPFVIAS
                     GNGIGRLLQLCQGFEDRLIFVADNGAHVYQNGKTVIRRAIQQVEVEAILHFFKGRWAD
                     VCLMLSNDGNIYMQAGAGMPFAGTDLPIESAQMVAFQNRVTYLDDLSAYPNFEPIYKV
                     GLWVPEARVESITEEFNQAFQGRLTAVTSGYGSIDILPEGIHKAWGLEQVLKDLVIQP
                     DQVMAFGDSDNDIELLSYVGYSYAMENATDKVKAVAKYMAPSHLEAGVLQVIEEYIL"
     gene            complement(4957..6189)
                     /locus_tag="ACHU66_000679"
     CDS             complement(4957..6189)
                     /locus_tag="ACHU66_000679"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFR3388982.1"
                     /translation="MERRANYIRRKRYASYRTKMHLFFICGLATIILAIIIVSEHLVP
                     RLNQASESMHSDNSVRIMAHGDLLYHLPILRGAEQPDGSYDFSENFTYVKPWIEQADF
                     AIADFEGTISPDIELAGYPLFNAPAIVANNIHDAGYDLVDLAHNHILDSHLSGLISTV
                     NTFRDAGVDTVGVYAEGNRATAPLYIREVNGIRIAVLAYAYGFNGMEALLSQEEYDAY
                     LSDFDKEKMRAEIERAEKEADITIVMPQTGVEYQLEPTEEQTSLYHQMIDWGADIVLG
                     GHPHVVEPTEILEKDGQRKLIIYSMGNFLSNQRIESMEDTPNAQWTERGVLMDLTIQK
                     ENGQTSIQTAQAHPTWVNRLSKGTYSTDGYEQFSYQTYILEDWVAGGRYYGQLDLDTQ
                     ARVDTVYQEMKDFVNLVW"
     gene            complement(6522..7460)
                     /locus_tag="ACHU66_000680"
     CDS             complement(6522..7460)
                     /locus_tag="ACHU66_000680"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFR3388983.1"
                     /translation="MVSTATTLGGFTFDNCLMNAAGVWCMTKEELDAVKNSKAGTFVT
                     KTATLDYRAGNPEPRYQNVPLGSINSMGLPNQGLAYYLDYLLELQETESERTFVLSVV
                     GMSPDETHEILRTVEASDFKGLTELNLSCPNVPGKPQIAYDFETTETILQEVFTYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNQFPLKFVNCVNSIGNGLYIKDESVVIKPKNGFGGI
                     GGEYIKPTALANVHAFYQRLKPEIQIVGTGGVLTGRDAFEHILCGASVVQVGTTLQKE
                     GVAAFGRITAELQAIMAEKGYETIEDFRGKLQHLDD"
     gene            7683..9029
                     /gene="gdhA"
                     /locus_tag="ACHU66_000681"
     CDS             7683..9029
                     /gene="gdhA"
                     /locus_tag="ACHU66_000681"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR3388984.1"
                     /translation="MSNAKAYIQASFEAVKARNPHETEFLQAVEELFSTLEPVFEAHP
                     EYIEENILARIVEPERIISFRVPWTDKYGNVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQSILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEIMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYMYGQYKRLRQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     FTDNMLAANGKSFKDQTVLISGSGNVAQYAVQKATELGAKVISVSDSNGYIIDETGID
                     FDLLVDIKEKRRARLTEYATEKATAKYFEGSVWNYDGKADIALPCATQNEINGEQAAA
                     LVKNGVYCVAEGANMPSDLDAIKVYKENGVLYGLAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMANIFNTAKETAEKYDLGTDYLAGANIAAFEQIADSMIAQGLV"
     gene            complement(9264..9851)
                     /locus_tag="ACHU66_000682"
     CDS             complement(9264..9851)
                     /locus_tag="ACHU66_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFR3388985.1"
                     /translation="MQTKDFVVENGQMYYKKKPLHKQPLFWTTIAGAVLSFILGVTCF
                     IFMIGLSSTNFDNWDTGSDGYIDETVEYTEYQVGDSVDFSDGLHVTVTSMGKDDSIEL
                     VDSYYSTAYVVEMEVENSTAKELYFDEYYFSLMDPVSQIPFTLDLRTYDVNLVEKLKP
                     GEKVQVKLIYGIDNETNFGFTYEDAMWTELVAEGI"
     gene            complement(9862..10710)
                     /locus_tag="ACHU66_000683"
     CDS             complement(9862..10710)
                     /locus_tag="ACHU66_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFR3388986.1"
                     /translation="MNDFITKYLSQFNIEAIVTAGLNKVLSLVLLFVAFYIIKKIAKA
                     SVKKVLVPSLKVSTQDIGRQKTISRLVESILNYLLYFILIYCILSILGLPVSSLLAGA
                     GIAGVAVGLGAQGFLSDLVNGFFILIERQFDVGDVVKLTNGPITISGTIVSMGIRTTQ
                     VRDADGTLHFIPNRNILVVSNQSRGDMRAQIDIPLKFNTDLEQVYRVIEEVNLREISN
                     FDQITGVTVLGPQNAISGQFVFRVNLFVANGQQNKIYHQFFGFYQDALRQAGIDLPSS
                     GPTVLK"
     gene            complement(10775..12109)
                     /locus_tag="ACHU66_000684"
     CDS             complement(10775..12109)
                     /locus_tag="ACHU66_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFR3388987.1"
                     /translation="MLELFKAINNLVWGPPLLLLLVGTGVYLTLRLGVFQIGKLPTAF
                     RLIFSSDQSGQGDVSSFAALCTALAATVGTGNIVGVATAITTGGPGALFWMWVAAFFG
                     MATKYAEGFLAIKYRTKDANGQAAGGPMHYITLGMGQKWKPLAVFFAISGVLVALLGI
                     GTFSQVNSITASLETSFGLEPPLVSVITAISIAFVVFGGIEKISDVSTKIVPFMAILY
                     ILASITVLAVHWDQLLPTLALVFKSAFTPAAAVGGFAGATVQQAIQRGIARGVFSNES
                     GLGSAPIAAAAAKSDNPVEQGLISMTGTFIDTLIICTLTGFTILVTDQWSLEGLAGAP
                     LTQAAFATVFGNTGSIALTISLVLFAFTTILGWSYYGERCIEFLFGTKAILPYRLVFV
                     AMVALGGFLKLDLIWTIADIVNGLMALPNLIALLALSPVIIKETRQYFAKKK"
     regulatory      complement(12215..12319)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(12532..13164)
                     /locus_tag="ACHU66_000685"
     CDS             complement(12532..13164)
                     /locus_tag="ACHU66_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3388988.1"
                     /translation="MFSIQSIQKKFGKRSLFSDVTLDLQAGKVYALIGASGSGKTTLL
                     NILAKLESYDAGDILYKGKDLKKLKPHLFFREELGYLFQNFGLLESQTIKENLDLGLV
                     GQKLSKDERKSRQEAALDAVGLSYLDLSQPIYELSGGEAQRVALAKVILKNPPLILAD
                     EPTAALDPKSSQEIMDILLSLKSPERLIVIATHNPLIWERADLIIKMEDL"
     gene            complement(13166..15148)
                     /locus_tag="ACHU66_000686"
     CDS             complement(13166..15148)
                     /locus_tag="ACHU66_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFR3388989.1"
                     /translation="MKGKFVLVSTIILAVFMIWLGVSQKETMLVHYYPSVTSLSVSED
                     VTYSDVRQRLEEYSQQTDSVIARRVIEPSTSGGRTFSYDNFSQSSLPRGLEEFQASEK
                     VESALLTKYFIFQGKATVEELRSLLVSLGFDEVQIRKPSTIATLLAFLTQGGQFLAVL
                     VFLITYMALVVVANVRQLRTAGIRLIAGDSRWHLFLLSLQENAKEIALTIPFAVLPAV
                     GLAYLIGLDSYSVYYLVAALVGYHFLLGLIALFFAATFTLAIRIYHFLPLLKGKMPLQ
                     GILTIMVMGQMLALLVVSLGMAQTVYYSGIWQEYQAGARQWEKEEDYYSLAWNIAADG
                     HSGLNSPENWYPLLKQALEKDGALFVKSNLNAYLMGSQLEDGTSLDSYHPAGNTLYVS
                     PNYLQIQDVDLSDEALPSLQEGEFQLLLPEKLRPESDIYLHLYQDYINRMVRPANQAS
                     SATIKGKLAYLKDGQKQFIYNHRSSQHVQYLTDPILVVLAPSSLGKESADFWLNEVDS
                     SILFKNRDKLLRELKARNLFQFVNEVRSSSSLFTELLDRIRIETISTSMGAILGIVTS
                     IILFNTMNLLYFEEFKRDIFIKEIAGMDFLGIHKKYLTFQLLSLLLALGASMVVTGHM
                     FISSISFALFMVNALCLLRWQARKDGTWNIRILKGA"
     gene            complement(15168..17153)
                     /locus_tag="ACHU66_000687"
     CDS             complement(15168..17153)
                     /locus_tag="ACHU66_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFR3388990.1"
                     /translation="MKKVFTYLSTFSVCLFCIWLVSLQLDSLIYHYYPSVSILSRGEE
                     VTYEEVSDALTNLAEKENSLIAKTITETDDSGRTTTSYDVFGKGELPQGLVKADRLVA
                     SQANLAENYYVVKGNLDIKVVENVLQSLGLVDSYIYRPTLLNSLSLFLGRGAPSLGLI
                     IFILTNLALTVVGQTVYLRSAGIEMLSGVRSWQIASRFLRVDGRDIALSTGGALVFGL
                     IAILILGWPMVYYRFVAIGIAFYACLLFFLSLSTSIGFMIALNISQLQKLIKGALPLK
                     AIFAILLVGQCLAVCIISIGASRTLTYVGVLHTLTIGEKAWQERQDVFSLSLNREAFI
                     ADEAMVQQQRMAWSHVILESYAKNNVMLVQHHLRHNTIKEHTIIESSKEIPLERVLYV
                     TPNYIQSEGGLCDFNQLEEIKQLGKGEIALLLPKSLQNDASVYQAYFEDMVGKLLADG
                     EKSISLYSNVYYISDEKRWFIYNHTPINYEQFLQAPLIVVLSPESFEETSYFWENALP
                     DFVFFKDKEMLEQLLEKYNLRNTIGSLLSSRQQYNTLRKNVQLEILMTLSPTILGIFT
                     SILLFNTMNLLYFETFKREIAIKRIAGMRFIELHGSYLGEQVGSALVGMGLAMFMTKS
                     VIVSLGVVVALLINSWMLLNKQAKKAEKIQLSVLNGR"
     gene            complement(17528..17821)
                     /locus_tag="ACHU66_000688"
     CDS             complement(17528..17821)
                     /locus_tag="ACHU66_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388991.1"
                     /translation="MDLNDYYTDIDPDFSFRELRRKIERYRIVMQARVLLDKRDTIKR
                     TGKTDIQKIASETKQTIQTMRGRLETSIKGSVRTGIEEAVDKHSSLYDTITQS"
     gene            complement(17808..18197)
                     /locus_tag="ACHU66_000689"
     CDS             complement(17808..18197)
                     /locus_tag="ACHU66_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388992.1"
                     /translation="MESDVIWERIRKREQELFDLEDDYNQEKNKIEARQEDLEQRQNA
                     LKLLIEREQEEMRCFLSRHSLDYDAALSFFQELDQLQEESFYQYRQEMDQLFQQEERL
                     SQQYRTDLYRLEDTISQLRRDYSNGLE"
     gene            complement(18182..19396)
                     /locus_tag="ACHU66_000690"
     CDS             complement(18182..19396)
                     /locus_tag="ACHU66_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFR3388993.1"
                     /translation="MISDKDYRDISKVVYDVDLLKAPKTPLIQDTPVVNGSFIIIEPP
                     VDAGNGMQAMAVAPIKGYDTDNKPIPDTSQVVIAYAGTNDGDIKDWATDISMIGGGNT
                     ATFTVPGQNVPYISQSITAVAFADQIVQRYPDATITITGHSLGGSLAMYVALKKDLPA
                     ITFNGPDIHNMISEDEIEYMQQHRAQFRNYRNKYDFLGDITGNKTKSAIYPAVKNIPG
                     DAIADHMLNAWKFNKYGQLIDANGQVVSTFTGAPLEDTRLAMKRWKKQKTALSKGGLS
                     RSEEIFLDSEQARTISAGIASVADAGQMSIFAHCDDYVHQAEELWNSIDFHSYSALSY
                     WEVCALFSEYGVSQETIVREIEETMDIYRQRAEIATEKFTILDTQIIQAINTMLETDK
                     RLAGDFEKWNQM"
     gene            complement(19393..19794)
                     /locus_tag="ACHU66_000691"
     CDS             complement(19393..19794)
                     /locus_tag="ACHU66_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388994.1"
                     /translation="MSKKCPRLILVMSAICIAIGGMIMFSFHRMSEEEKLQAQIRKEQ
                     ERMVLYAVNHYEGIEKIEFVNFEKDNKTGTWDSDAIINDKFHVTFVSWGEDDITINGG
                     KSQTGDYLVPKVATTVTEISNIQVKYYKELP"
     gene            complement(19820..20095)
                     /locus_tag="ACHU66_000692"
     CDS             complement(19820..20095)
                     /locus_tag="ACHU66_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388995.1"
                     /translation="MITDQDYRWIADDVYKVDPLKTDDTLKQGGRAGQGQYVIIEPPV
                     DAGNGMQAMAVAPIKGYDSDNKPIPDTSQVVIASFPYPDSSVNTEYL"
     gene            complement(20095..20505)
                     /locus_tag="ACHU66_000693"
     CDS             complement(20095..20505)
                     /locus_tag="ACHU66_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388996.1"
                     /translation="MSPKRTRLILVMSAICIAIGGMIMFSFHRMSEEEKLQAQIRKEQ
                     ERMVLYAVNHYEGIEKIEFTSFRENRMTGAWYAGAILNDDYKVTITAMGFDGDLSMND
                     GPSDKTGLYLRIKDKPTTISNPNHIEVIYFKGDN"
     gene            complement(20583..20816)
                     /locus_tag="ACHU66_000694"
     CDS             complement(20583..20816)
                     /locus_tag="ACHU66_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388997.1"
                     /translation="MITVQDYRWIAEDVYKVDPLKTDDTFKDGDRVAQDKFVILSQPQ
                     DMINGMQAMAVAPIKGYDAENKPIPDTSQVVIA"
     gene            complement(20813..21160)
                     /locus_tag="ACHU66_000695"
     CDS             complement(20813..21160)
                     /locus_tag="ACHU66_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3388998.1"
                     /translation="MMFNFHRMSEEEKLQAQIRKEQERMVLYAVNHYEGIEKIEFTSF
                     EENKMTGSWSAGAILNDDYKVTITAMGFDGDLSMTNGPSDKTGLYLRIKDKPTTISIP
                     NQIEVIYFEGDNQ"
     gene            complement(21349..22290)
                     /gene="msrB"
                     /locus_tag="ACHU66_000696"
     CDS             complement(21349..22290)
                     /gene="msrB"
                     /locus_tag="ACHU66_000696"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR3388999.1"
                     /translation="MKEIYLAGGCFWGMEGYFSQIDGILDTSVGYANGQVETTNYQLL
                     KQTDHAETLYLAYDETRINLREILLYYFRVIDPFSVNQQGPDKGRQYRTGIYYTDKAD
                     LPTIEQVMMEQSQLFGGRPLAVEVEPLAHYIPAEDYHQDYLKKNPQGYCHIDLGQAKI
                     PLIDVADYQKPDQQVLKDSLTDLQYQVTQEAATERPFENEFWNSDQAGIYVDITTGEP
                     LFLSTDKFDSGCGWPSFTKPISKEVATYFQDKSHGMNRIEVRSRAGHAHLGHVFDDGP
                     RDKGGLRYCINSAALRFIPREEMEAAGYGLFLDLLSE"
     gene            complement(22330..23859)
                     /locus_tag="ACHU66_000697"
     CDS             complement(22330..23859)
                     /locus_tag="ACHU66_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFR3389000.1"
                     /translation="MSTISYKPLNLYRQFKTAAQEFPNVAIYFDKPYASFPELGLENT
                     YQTVFEAIQKRAAQFSAAGIGYGDKVILYKSPAFDTYLLAVAVTALGAVPVMISYHLP
                     SSNLDVFAERLEKSFIVYDQETEERVAGMTRTDLVTKVFLPQVLVQEAVAFEENLLPE
                     DVIQYMTHTSGTTGVPKLICHTAQTMGWRVAWQQTIFDKMTERGLLAFHISPVHSRYN
                     IGVSSAIGLGFPLYPLSSARKDDVERALALHRPSALETHPNNFVQWARLAKEKPEVFS
                     SIRYYHSTFDAINIGTLRAFLEASKEQHPVFMQVYGQSECGPMILRYHRLENLGTVSG
                     RDMGIGLEGYTEARITDAQGNPLPAGENGHIQFLSKGRAVTYYKEDARFQDNVYGAWW
                     DSGDYGCMTPEGTLLLKDRQVDLIEHIDSNLALEDLLLDKLDFLSEVVIIRDVNGAPQ
                     PIIALVEDAEMNWEAWWAMVADLPLLKEPILMAYDDIPRTATMKVQRLKMEAEMKEKN
                     L"
     gene            complement(23992..24306)
                     /gene="trxA"
                     /locus_tag="ACHU66_000698"
     CDS             complement(23992..24306)
                     /gene="trxA"
                     /locus_tag="ACHU66_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFR3389001.1"
                     /translation="MVQVITDANFEVETQEGVVLVDFWAPWCGPCRMQAPILEQLADE
                     VDEDELRIYKMDVDENPNTARQFGIMSIPTLLFKKDGQVVKQVAGVHTKNQIKAILAE
                     IG"
     gene            complement(24546..24866)
                     /locus_tag="ACHU66_000699"
     CDS             complement(24546..24866)
                     /locus_tag="ACHU66_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389002.1"
                     /translation="MHQLYQMLQDLYTKADQIDYATINAIEKEIERWLIRSIYAPLKL
                     REKNAILKQHKKKIQTIGNDGENSVKLIANSFDTSIKGEFSKKVQQVVKEKAKEYTKL
                     EEVE"
     gene            complement(24879..25265)
                     /locus_tag="ACHU66_000700"
     CDS             complement(24879..25265)
                     /locus_tag="ACHU66_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389003.1"
                     /translation="MDQYDKLKAELMKKEWEWEDIENQQRKAQKELQEHYENVEETTR
                     ILTRMLEEKYQEVLLELRQVGDETGDLHHLLNNGMSEWHTAIDQERYSSIHRLDQKQE
                     DLDTYYKNQYRKMQDQIDEIYTKYRE"
     gene            complement(25244..26503)
                     /locus_tag="ACHU66_000701"
     CDS             complement(25244..26503)
                     /locus_tag="ACHU66_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mbeg1-like protein"
                     /protein_id="HFR3389004.1"
                     /translation="MNNSKTNRDLTDFDRQQVASEEYTPYLKGNEVVITNFFEEELTI
                     GTVRKVLKDATGLDGYVVEEPDGNVIVLFQGSKGPGKEGAAADWMDNDLPMTHNIISN
                     KSEVTPQLQSASRILNQVLKDYPNAQITVYGHSLGSMNAQYALAAVSDIDRIAGAYIY
                     NGPNVYPALTEAGKARVNALKYRIHNYIDQKDFVPIGYSGKDAPGYKSTAGTSEGAIG
                     IVYRVDSKTNLNPIDQHVWGGYQWNADGSLKVKEGSSKLEQHYSNALHHVSSEMYHYA
                     TLKATLSRGGFSSRETIYLDSEQARILAQGLVKVAETTHQTLEKETTSTLTEVNEVYS
                     SLGNVPFGFILSPDEVRQAYSSAGVDYHSLVGDSTNQVEKFITRSNQLKQDLVDLESQ
                     IQAGIEQKVTEDQTLAQRIQEWTSTIN"
     gene            complement(26496..26885)
                     /locus_tag="ACHU66_000702"
     CDS             complement(26496..26885)
                     /locus_tag="ACHU66_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 family protein"
                     /protein_id="HFR3389005.1"
                     /translation="MKIVVKILVGILIAIAIGIGGFKLMQKIEHDQMVEIVKGEEVRK
                     MVEERLISYDAKALTEEGIIRSYSIDSASIRRNPMGGINFSVYLNNDDTLYINYRIEK
                     IRDDIVKLVGGGKSSDLTRLLGEEANE"
     gene            complement(26980..27237)
                     /locus_tag="ACHU66_000703"
     CDS             complement(26980..27237)
                     /locus_tag="ACHU66_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389006.1"
                     /translation="MTEYNNLGKGDAVITQYDDIVGRVTLDFDNENGNGEQVYAVVKD
                     PNLPEDQVTEVMVLFRGSTGSNEVLTKPADVWNDWGESILS"
     gene            complement(27284..27661)
                     /locus_tag="ACHU66_000704"
     CDS             complement(27284..27661)
                     /locus_tag="ACHU66_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 family protein"
                     /protein_id="HFR3389007.1"
                     /translation="MKKVMKVIIGILLVIGIGIGGFVYMQHKEHEKMVAIATSEEARA
                     VYEKHMKANDPKALTADGIIKTYEIDTETLEYNPMGGMIVRVYVNGDEDFGFHFNLLQ
                     NEDGKFKSGSYVIKSKLADLLGK"
     gene            complement(27687..27950)
                     /locus_tag="ACHU66_000705"
     CDS             complement(27687..27950)
                     /locus_tag="ACHU66_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009569026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389008.1"
                     /translation="MTEKYTDRDRQKIANKEYSAYKFGDEVNIGTLEHPRYIGTVRQV
                     LKDATGLDGYVVEEPDGNECCDGDGNTVSAASMLYYEVRKHLT"
     gene            complement(27943..>28276)
                     /locus_tag="ACHU66_000706"
     CDS             complement(27943..>28276)
                     /locus_tag="ACHU66_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1310 family protein"
                     /protein_id="HFR3389009.1"
                     /translation="GIKLMQKIEHDQMVEIVKSEEVREIVEEQLKYLDNEALSEDGLI
                     KTYEIDDSSIIHSPMGGISFNVFLNSDRKLRVVFELEKDSNTGKIEYSGGGYSSEVKK
                     LVGEINND"
BASE COUNT         8105 a         6482 c         5181 g         8508 t
ORIGIN      
        1 tgctccgcac ctgaagcaag acttgctact ccatttgtgt aggtcgttaa gccactggac
       61 agtgtttgac tgcctatcgc agcctgattg actccatctg tataagacac aactccggtg
      121 gccaattggt ttgctcctgt cacgggtgat tgacccgaag tcgttaaagt agtcaatcca
      181 tttgaaagtg tttgactgcc tgactccaat tgactagctc catctgttaa ttgttgactt
      241 ccatctgccg cctcgaccat gccggtttga atttctctca tactatcaaa aacggcagtc
      301 gtgtaggttt tggtaattgt ctctgaaaca gatgtcttta atttctccat agctgattcg
      361 cccatcttag aagcaacaaa gctatgacct ttggaggttt gataagaaat cgttatcggt
      421 tcaggctgat tcgtcaagag actggttgct ttttctgata aatcttctgg tagagtgatg
      481 accatataat agtccccttc ttccaagcct ttctccgcat ccttttctga tacaaagtgg
      541 aaatccagac ttttgctctc tttcatatta tccaccatat catcaccaat ggtaagggtt
      601 ttatcttgaa agctcgccga ctggtcttga ttgatcactg ctacaggaag ctggtctagt
      661 cggccgtaag ggtcccacat agaggttagg aaactaatgt tatataaggc tgggacacag
      721 gcaataccaa taatggtaac caaagtcatt ggttttttga aaatattctt cacttttcgt
      781 aacataagca actcctttag acatactgtc caaaaactga taaaatacat tatacaagta
      841 tcattttcaa aatcaagtca taagaaccgt ttttagacac tgtgtctaaa aatgttcaaa
      901 aggagaaatt atgggaacag atagtcggaa agaacgaact cgacaggcaa ttttagaagc
      961 tatggtaacc tgcctggaat cacaaggatt taatgacatt acaacaacac acctagccca
     1021 gactgctggt atcagtcgtt ccagttttta tacccactat aaggataaat atgaattaat
     1081 tgactcctat caacagggac tttttcacaa attagaggct atatttgacc gttatgatgg
     1141 caatcgccaa tcatcatttt tagaaatttt cgagttactc tatcgtgaaa aattgctttc
     1201 tgccctactg acccataacg gaactcaaga aatccaaaat tttcttatca ataaagtccg
     1261 acttcttgtt gccaatgatc tagcggagcg cttggggaaa gaagatttat caccacttga
     1321 aaaagaatac agcagcatct atttttcaca tgccttcttc ggtatcatgc aggtatggat
     1381 taaaaatggt aagcaagaat ctcctcaata tatgaccgat tttctaataa aaatgttacc
     1441 ttaaaggtga ctttttaccg ccactttctg ggaaatatgt tataatatcc ttatcttacg
     1501 caaacgtttt caaacaggag attatgatat gattcaggca gaacatcttt caaaaaccta
     1561 ttcaatcatt gataaggaag tcggtctaaa aggctctatc aaggcatttt tcaagccaaa
     1621 gaaaaagtca atccctgctg ttcaagatat ttccttacag gtaggaaaag gagaaatcat
     1681 cggctatatc ggttccaacg gttctggtaa atccaccacc atcaaaatgc tgacaggtgt
     1741 tctctatcca gaccaaggac aggttcgtat caacggactc aatccgcaag aaaaccgcaa
     1801 agctgtcaat aagcaaatcg gcgttctctt tggacaaaaa tctcatttgg attggaactt
     1861 gcctgtccag gaatctttta tccttcacgc aaaaatttat gatgttccag ataaggtatt
     1921 caaagagcga ttggctgtct tgattgagct attggacttg gctgatatta tgaagcaacc
     1981 aatccgtaat ctgtcactcg gccaacgggt tcgctgtgaa tttgcggcta tctttatcca
     2041 ccagcctgcc gttgtttttt tggacgaacc aaccatcggc ttggatgcca gcgtcaagga
     2101 aaccattcgc tccttcatcc gctatatgaa tcaagaatac cagaccactt ttttgattac
     2161 ttcccatgat atgaaggaca tcgaaagcct ctgtgagcgg atttttatca ttgataaggg
     2221 caagaaggtc tacgatggtt cactgaccgt cctcaaagaa cgcttttcca cagtcaagac
     2281 cattctcttc tccacagaaa aaccaattga aaaagatttg caattggagg gctgggaatt
     2341 tattcgtaag gatgattttc actttgaaat ccactaccaa aacaaggtct ggaccagcgc
     2401 ccaggtgatt gagcaagttt tcaatcatta tgccattgaa gatgttacca tgaaggaatt
     2461 ggaaattgaa accatgctcc gtcagattta tgaggagggt atccatgcgt aaatatctct
     2521 acatgacccg cctgaccatg atgaatgcta tgcagtataa ggctttcttt ctagcgactt
     2581 tcgtttcact agcagtgaag attttggtag ctctctatgt ctggaaaacc attttcttca
     2641 ctcaatcaga agtcaatggc tttaccctac aaacctttac tacatatatc atctttgcta
     2701 atctactagc cagtttaaat tctttctcac tcggagaaga tttatcctac agcattttaa
     2761 aaggaagcat tgctggagaa tttcttagac cctattcctt tatcctagcc ctctttttca
     2821 aagaccttgg aaataagctt ttggaactga taaaatttgc tatcgtattt atcggtatct
     2881 tgcttgttca tcaggatttt tatctacctg acggaaaaac tattttactc tttctagtca
     2941 gctccatcct cggtatgttt atcgttcaat tgctggatat ggcttttggc ttcttagcat
     3001 tttttacggt caatgcctgg ggagtcatgc ttctgagaat ggggcttttc aatctagctt
     3061 ctggagcgct tttgccactc agcttctatc cacaggctgt ggaaaacttt ttgaagctcc
     3121 ttccctataa ttatgcggtt aacgttccag tttccatttt attgggacag gaaagcgatc
     3181 taactgcttt gggattgcaa gttatatgga tacccattct agcctgcttc atcgctctac
     3241 tctggtccca ggccaagcgt cgtattgtca tttttggagg ttaacatgaa gaaatatatc
     3301 agcctctatc tatataacat caaggtctac atgatggccc agatgtcctt tcgagtggac
     3361 ttcttcatcg ggcttttctc ctctttgatt gaacaaattg tctacctgat tttcctcaat
     3421 attctctttg gaaatatcaa ggaaatcgct ggttttaact acggtcaaat gcttttcatc
     3481 tatggaatag cgaccgtcgg acgctccatt cacttgattt tctttgacaa tctctggatg
     3541 tttggtagtc gctacatccg acaaggcgaa tttgaacgcc tcctcctcat gcctgtgaac
     3601 ccactttttc aacttatctg tgaacaaatt cagccacaag gaatcggaac aaccctcatc
     3661 ggttcccttg cactagtcca agcttccaag gaactgggta tggaatggag tcttggaaaa
     3721 ctagccttac taatctttat tgccatttgt atcgggctac tctatgcagc cattcaactc
     3781 ggtccaactg ctctagcatt ttggattgtg gaatcatttc ctttgactat gggaattttc
     3841 gccctcaatc agatggctca atacccactc aacatctatc caaaaatcat tcaaatcctg
     3901 ctgatatttg ttttccccta cgcttttacc gcttatttcc cagccctcta cttcttagac
     3961 ttgtctatgt ggggactggc gttgcctctg gtggtagtgg tcctattttc catcaattat
     4021 aacctcttcc gctatggtat gaccaagttc accagtgttg gaaattaaaa aaagctagaa
     4081 ttctctggat tgagagcact agcttttttc tatatactta taaaatatat tcttcaatga
     4141 cctgaagcac acctgcttcc aaatggcttg gagccatgta tttggcaacc gccttgacct
     4201 tgtctgtcgc attttccatg gcataggaat acccaacata ggacaataac tcaatatcat
     4261 tgtctgaatc accgaaggcc atgacttgat caggttgtat taccagatcc ttcaaaactt
     4321 gttccaagcc ccatgcctta tgaatccctt ctggcaatat atcaatggag ccatagccac
     4381 ttgtcactgc agtaagacga ccttgaaaag cctggttaaa ctcttccgtt atcgattcta
     4441 cacgcgcttc aggaacccag agaccaacct tataaatagg ttcaaaatta ggataggcac
     4501 tgaggtcatc taaatatgtc acacgatttt ggaaggcaac catctgagct gattctattg
     4561 gcaaatcagt ccctgcaaat ggcatacctg ccccagcctg catataaata tttccatcgt
     4621 tcgacaacat cagacataca tccgcccaac ggcctttgaa aaaatgcagt attgcttcga
     4681 cttctacttg ctgaatagcc cgacgaatta ctgtcttgcc attttgatac acatgggctc
     4741 cattatccgc tacaaagatt aatctgtctt caaatccttg gcataattgt agtaatcgcc
     4801 caattccatt accactagcg ataacaaaag gaatcttttt ctgatctagt tgatccaaga
     4861 cccgtgaaaa tcgctctcta tcaaaattcc cctgaccatc taaaaaggtc ccatccatgt
     4921 ctgttgcaat taatcgaact atcatcttct ttctcctcac cataccaaat tgacaaagtc
     4981 tttcatttct tgatagactg tatccacacg cgcctgtgta tctagatcca attgtccata
     5041 gtatcgacca ccagcgaccc agtcttccaa aatgtaagtc tggtaggaaa attgttcgta
     5101 tccatctgtt gaataagtcc cttttgaaag gcgattgacc caggttggat gggcttgagc
     5161 cgtttgaata cttgtttgcc cattctcttt ctgaatagta aggtccatca agacacctct
     5221 ttctgtccac tgggcatttg gggtatcttc catactttcg atacgctgat tggatagaaa
     5281 attccccata gaatagatga ttaattttct ctgaccatct ttctcaagaa tttccgtcgg
     5341 ctcgactaca tgcggatgcc caccaagaac gatgtctgct ccccaatcaa tcatttgatg
     5401 gtacaaactg gtctgttcct ccgtaggctc caactggtat tctacacccg tctgaggcat
     5461 gacgatggta atatctgcct ctttctcagc tcgctcaatc tctgcccgca tcttttcttt
     5521 atcaaaatcc gataaatagg catcatattc ctcctgactc agcaaagcct ccatcccatt
     5581 gaaaccgtaa gcataggcta ggacagctat acggatacca ttgacttccc gaatgtagag
     5641 tggtgcagtt gcccgattgc cttcggcgta gacaccgact gtatccacac ctgcatctct
     5701 aaatgtattg acagtggaaa tcagccctga tagatgtgag tccaagatat gattgtgggc
     5761 taagtccacc aaatcgtagc ctgcatcatg gatattgttg gcgacaatgg caggcgcatt
     5821 aaaaagtggg taacctgcta attcgatatc tggagaaatg gtcccttcaa agtctgcgat
     5881 agcgaaatca gcctgttcta tccagggctt tacgtaggta aaattctcag aaaaatcgta
     5941 acttccatct ggctgttctg ctcctcgaag aataggcaag tgatagagca aatccccatg
     6001 agccatgatg cgaacagaat tatctgagtg cattgattct gaggcctgat tcagcctagg
     6061 aactaggtgc tcgcttacaa taataatcgc caagataata gtcgctaagc cacaaataaa
     6121 aaacaaatgc atctttgtcc gataggacgc atatcgtttt ctgcgaatat agttcgccct
     6181 tctctccata cctttaccct catactccct acttaagaac ctttgttcaa aatacagtca
     6241 gcagtccctc aaaaaactat ttcttccata aaagcacatc acttataaat agaggttcta
     6301 atattagtat acgatatttt tgctaaaaaa tctaaataaa atcagaattc catctcacaa
     6361 atatatgttt ctgactatgt attccattcc tcaaacgatt atcagaccga tcttcacaga
     6421 ccatttaaaa agagctaaca ttatcccctc gcgactccct aaaagactat ctgggagaac
     6481 agtacaatgt tagcttcttt ttatgtcttc aacagatgat ctcagtcatc taagtgctgt
     6541 agcttgccac gaaaatcttc aatggtctcg tagccttttt cagccatgat ggcctgcaat
     6601 tcagccgtaa tgcgtccaaa ggctgccaca ccttcctttt gcagggttgt tccaacttga
     6661 accacgctag ctccacagag aatgtgttcg aaggcatccc gacctgtcaa gacgccacca
     6721 gtcccaacaa tctgaatctc cggtttcaag cgttggtaaa aagcgtggac attggcaaga
     6781 gcagtcggct tgatatactc tccaccgata ccaccaaaac catttttagg cttgatgaca
     6841 actgactcat ccttgatata gagaccattt ccaatcgaat tgacacagtt gacaaacttc
     6901 aaaggaaact ggttgaaaat cgctgctgct tggtcaaaat gcacaatatc gaaataaggt
     6961 ggtaacttaa taccgagtgg tttggtaaag taagtgaaga cctcctgcaa gatggtttct
     7021 gtcgtttcaa aatcataggc aatctgcggt tttccaggga cattgggaca agaaagattt
     7081 aactctgtca gccccttaaa atcactggct tctacagttc tcaaaatttc atgagtctca
     7141 tcaggcgaca ttccaactac agataaaaca aatgtgcgtt ctgactccgt ttcctgcaat
     7201 tctagtagat aatccaaata gtaagccaag ccctgatttg gcaaacccat cgaattgata
     7261 gaaccaagcg gaacattctg ataacgtggt tctggattac ccgcgcggta gtccaaggtt
     7321 gctgtttttg ttacaaaagt acccgccttc gagttcttaa ccgcatccaa ttcttccttg
     7381 gtcatgcacc aaacccctgc cgcattcatc aaacaattat caaaggtaaa tccaccgagt
     7441 gtcgttgctg ttgaaaccat attatcctcc atataggtca ttctttcttg attatatcat
     7501 aagaaacaaa cggattttcc ccatttacta ctaacgttcg gattttgcaa taaaaattta
     7561 ttaatttttt taaaattctt acattttctt ctttacattt aaaaatcatg gtgctataat
     7621 aattatcaga taaattaaat tgtcagataa tttaataaat aaaaaagaaa tcaggtgatc
     7681 ccatgtcaaa tgccaaagct tacatccaag cttcttttga agcagtcaaa gcccgcaacc
     7741 cacatgaaac agaattcctc caagctgtag aagagctctt ctctacactt gagcctgttt
     7801 ttgaagcaca cccagaatac atcgaagaaa acatcctggc tcgtatcgtt gagcctgagc
     7861 gtatcatcag cttccgtgtt ccatggacag ataaatatgg aaatgtccaa gtcaaccgtg
     7921 gctaccgtgt tcagttcaac tcagctgtag gtccttacaa aggcggtctt cgcttccacc
     7981 caactgtaaa ccaatccatc ttgaagttcc tcggttttga acaaatcttc aaaaacgtct
     8041 tgactggtct tccaatcggc ggtggtaaag gtggttcaga ctttgatcct aaaggcaaaa
     8101 cagacgctga gatcatgcgc ttctgccaaa gctttatgac tgaattgcaa aaacacatcg
     8161 gaccttcact tgacgtccct gctggtgaca tcggtgtcgg tggtcgcgag attggttaca
     8221 tgtacggtca atacaaacgt ctccgccagt ttgatgcagg tgtcttgact ggtaaacctc
     8281 ttggctttgg tggttcattg atccgcccag aagcaactgg ttacggtttg gtttacttca
     8341 ctgataacat gttggcagca aacggtaaat ccttcaaaga ccaaacagtc cttatctcag
     8401 gttctggtaa cgttgcacaa tatgctgttc aaaaagcgac tgaacttggt gcaaaggtta
     8461 tttctgtttc agactcaaat ggttacatca ttgacgaaac tggtattgac ttcgacctct
     8521 tggtggacat caaagaaaaa cgccgcgctc gtttgacaga atacgctaca gaaaaagcaa
     8581 ctgctaagta cttcgaaggt tctgtatgga actacgacgg aaaggctgat attgcccttc
     8641 catgtgcgac tcaaaacgaa atcaacggtg agcaagctgc tgcccttgtg aaaaatggcg
     8701 tttactgtgt ggctgaaggt gccaacatgc catctgacct ggatgctatc aaagtttaca
     8761 aagaaaatgg cgttctttac ggtcttgcaa aagctgccaa cgctggtggt gtagccgtat
     8821 ctgcccttga aatgagccaa aacagccttc gcttgtcatg gactcgtgaa gaagtagacg
     8881 gccgtcttaa agacatcatg gcaaatatct tcaacacagc caaagaaact gctgaaaaat
     8941 acgaccttgg tacagactac cttgccggtg ctaacatcgc agcctttgaa caaattgcgg
     9001 atagcatgat tgcccaaggt ttggtataag cttactttta gtttactaaa ctataataaa
     9061 aacgccccca aaattgacgt gctaattttg ggggtatttt tcattaaaag aagaggagaa
     9121 cagtggataa acatgttctc ctttttaaaa tgtaaaaata acagataagt cggatatagt
     9181 acaattacca agccgaacaa tctgaaccaa ggggcgtata aacccgcaaa attgtgcaat
     9241 atttaaaact ttttctacag caattagata ccttctgcga ctaattccgt ccacatggca
     9301 tcttcgtagg taaaaccaaa gtttgtttca ttatctatac cgtagataag ttttacttgt
     9361 actttctctc ctggcttcag cttttcaact agattcacat cataggtccg caaatccagg
     9421 gtaaagggaa tctgactaac gggatccatg agactgaaat agtattcatc aaaatagagc
     9481 tcttttgcgg tcgaattttc aacttccatt tcaacaacat aagctgtgct gtagtagcta
     9541 tccactagtt ctatgctatc gtctttgccc atagatgtta ctgttacatg aagaccgtca
     9601 gaaaaatcaa ctgagtcacc aacttgatac tccgtgtatt ccacagtctc atcaatgtat
     9661 ccgtcactac ctgtatccca attatcaaaa tttgttgagc ttaggccgat cataaaaata
     9721 aaacaggtta ctcctaagat gaaggagagg actgcaccag caatggtcgt ccaaaagaga
     9781 ggctgtttgt gcaagggttt cttcttataa tacatctgcc cattttcaac gacaaaatct
     9841 tttgtttgca taataaactc cttattttaa aacagttggc ccacttgaag gtaaatctat
     9901 tcctgcttga cgcagagcat cttgataaaa accaaagaat tgatgataga tcttgttctg
     9961 ttgcccgttg gctacgaaaa gattaacacg aaatacaaac tgtcccgaaa tagcgttttg
    10021 gggacctaat acagtcacac ctgtaatctg gtcaaaattg ctgatttctc tcaaattgac
    10081 ctcttctatt acgcgataga cttgctccaa atcggtatta aatttgagcg gaatatcgat
    10141 ttgagcacgc atgtcccctc ttgactgatt ggacaccact agaatgttgc gattgggaat
    10201 aaaatgcaat gtcccatctg catcacgtac ttgtgttgtt cggattccca tgctaacgat
    10261 ggttcctgaa atggtaatgg gcccgttagt taacttaacc acatctccga cgtcaaactg
    10321 tcgttcaatt aagataaaga atccattgac caagtctgag agaaaacctt gtgcgcccag
    10381 cccaacagct accccagcaa tcccggctcc tgcaagaaga ctggataccg gtaaaccgag
    10441 gatactcaaa atacagtaga taaggataaa gtagagtagg taatttaaga tgctttccac
    10501 cagacgactg atggtcttct gacgaccaat gtcttgtgta gataccttta gagagggaac
    10561 caaaaccttt ttcacagaag ctttggctat cttcttgata atgtaaaatg caacaaagag
    10621 taaaacaagc gataaaacct tatttagacc tgctgtcaca atggcctcaa tattaaattg
    10681 agacaaatac ttggtaataa aatcattcat gagataatta tagcagaaaa caccctaaaa
    10741 gaacaaaaag acagccaagt ggctgacttt tatcttattt cttctttgca aaatactggc
    10801 gtgtctcctt gatgataaca ggagagagag ctagtaaggc gatcaagttt ggtagggcca
    10861 tgagaccatt tacaatatcg gcaatggtcc aaatcaagtc tagtttgagg aaaccaccca
    10921 aggcaaccat agctacaaag accagacggt aaggtaagat ggctttggtt ccaaatagaa
    10981 actcaataca gcgttcacca tagtaagacc atccgagaat agttgtaaaa gcaaaaagta
    11041 ccaaactaat cgtcaaggca atagatcctg tattgccaaa aacagtcgca aaggctgcct
    11101 gcgtcagagg agcacctgcc aagccctcta gcgaccactg gtcagttact agaatcgtaa
    11161 aacccgttaa ggtacagatg ataagtgtat caataaaggt acccgtcata gaaatcaagc
    11221 cctgttcaac tggattatca gacttggcag ccgcagcagc aataggcgcc gaacccaaac
    11281 cagattcatt tgagaaaacc ccacgcgcaa tcccacgctg aattgcttgt tgcacagtcg
    11341 cacctgcaaa accgcccaca gccgctgctg gagtaaaggc agatttgaaa actaaggcca
    11401 gggttggcaa gagttgatcc caatgcacgg ccaagacagt aatactagct aagatataca
    11461 aaatcgccat gaaaggaaca attttggtcg aaacgtcaga gattttttca atcccaccaa
    11521 aaacaacgaa ggcaattgaa atagccgtta ttacactgac aagtggcggt tccaatccaa
    11581 aactcgtttc caaagaagca gtgatggaat taacttgcga aaaagttcca atccccaaga
    11641 gagcaaccaa aacacctgaa atagcaaaga aaactgccaa gggtttccac ttctgtccca
    11701 ttcccaaggt gatataatgc ataggtccac cagctgcttg cccattggca tccttggttc
    11761 tatatttgat ggccaaaaag ccttccgcat acttggttgc catcccgaaa aaggccgcaa
    11821 cccacatcca gaaaagagcc ccaggaccac ctgtggtaat agctgtcgca actccgacaa
    11881 tatttcctgt accaactgtc gctgccaaag ctgtacaaag ggccgcaaag ctggacacat
    11941 ctccctgacc agactggtca ctggagaaaa tcagacgaaa agccgtcggc aatttgccaa
    12001 tctgaaatac tcccaaacga agagtgagat aaacacccgt tccgaccaat agtaacaaga
    12061 ggggcggccc ccaaacaaga ttgttgatag ccttaaatag ttctaacata gttcctccta
    12121 agcgtttcta atccgataaa gctgccggaa aaacaaaaag aggctgaaca cttcaacctc
    12181 tagaatcaac gtgaaaatat agaaaaagcc tatttttcaa cgctctgtcc ttttacctga
    12241 gagtttcagt agctctactt ttccaagcag gactactttg ccccttcggt gccaaaatgg
    12301 tctctccaga gttccgtcca ttgaacagtc agtttccctt ctacccaata tcaaccggtt
    12361 attcagtttt atctagtttt attttaatct aatttcacga aaaaatcaag aatattttct
    12421 gatactcaat gaaaatcaaa agtagcctag gaaacgaagc cgaagataga actggagttc
    12481 atcaaggtaa gttgacaacg gataattttg attttcgaag agtatgaatt tttacaagtc
    12541 ttccattttt atgataaggt ctgctcgctc ccaaatcaaa ggattatggg tcgcaatcac
    12601 aattaaccgt tctgggctct tcaaggataa caaaatatcc ataatttctt gggagctctt
    12661 aggatccaag gcagccgtcg gctcatctgc taaaatcaat ggcggatttt tcaggatgac
    12721 tttagcaaga gctacccgct gggcttcccc tcctgagagt tcatagatag gctgggacag
    12781 gtccagataa gacagaccaa ctgcatcaag tgccgcttcc tgcctcgatt tcctttcgtc
    12841 tttggacaat ttctgaccaa ccagtcccaa atccagattt tctttgattg tctgactttc
    12901 taaaagacca aaattctgaa agagatagcc taattcctcc ctaaaaaaga gatggggctt
    12961 caactttttc aaatccttcc ccttgtagag aatgtcacct gcatcatagg actccaactt
    13021 ggctaaaata ttcagcaagg tcgtttttcc gctaccactg gcacctatca aggcatagac
    13081 ctttccagcc tgcaagtcta gcgttacatc agaaaacaaa ctgcgtttcc caaatttctt
    13141 ttgaattgat tggatagaaa acatactagg ctcctttcaa aatacggata ttccaagttc
    13201 cgtctttcct cgcctgccac ctcaataaac agagggcatt gaccataaac aaggcaaaac
    13261 tgatactact tatgaacatg tgacctgtca ctaccatgct tgctccaaga gccaacaaga
    13321 gggaaagcag ttggaacgtt aggtatttct tatggattcc caagaaatcc atccctgcaa
    13381 tttctttgat aaaaatatca cgtttaaact cttcgaagta taatagattc atggtattaa
    13441 acaggataat tgatgtcaca atccctaaaa tagcacccat agatgttgaa atggtctcta
    13501 tccgaatacg gtctaaaagt tctgtaaaga gggaggaact acttctcact tcattgacaa
    13561 actgaaacag atttcttgcc ttcaattcgc gcaaaagttt atccctgttt ttaaataaaa
    13621 tactgctatc tacttcattc agccaaaagt cagcagattc ttttcccaga cttgacggtg
    13681 ccagcacaac taaaataggg tctgtcaggt attgcacatg ttgactagaa cgatgattgt
    13741 agataaattg cttctgccca tcttttaaat aggcaagttt tcccttgatg gtagctgagc
    13801 ttgcttgatt ggcaggtcta accatacgat tgatatagtc ttggtaaaga tggagataaa
    13861 tatcgctttc agggcgcaat ttttcaggta agagaagttg aaattctcct tcttgtagag
    13921 aaggtaatgc ttcgtccgac aagtctacat cctgtatctg caaataattt ggactaacgt
    13981 agagggtgtt gcctgctgga tggtagctat caaggcttgt cccatcttcc agttgggagc
    14041 ccattaaata ggcattcaaa ttacttttga caaagagcgc gccgtctttc tccaaagctt
    14101 gctttagtaa agggtaccaa ttttcaggcg aatttagtcc tgagtgacca tccgcagcaa
    14161 tgttccaagc caaactatag tagtcctctt ctttctccca ctgccgggca cccgcttggt
    14221 attcttgcca aatccctgaa taatagacgg tttgagccat gcccaatgac accaccagta
    14281 gagccagcat ttgccccata accataatgg tcaggattcc ttgcaggggc attttccctt
    14341 ttaacaaggg taaaaagtga tagattcgaa tggccaaggt aaatgtagca gcgaaaaaaa
    14401 gggcaatcaa accaagcaag aaatgatagc ctactagagc tgcgaccaga tagtagacag
    14461 aatagctatc taatccaatg aggtaggcca gtccaactgc tggaagaacc gcaaatggga
    14521 tagttagagc tatctctttc gcattttcct gtagagatag tagaaataaa tgccaacgtg
    14581 aatctcctgc gatcaaacgg atacctgctg tgcgcaattg ccttacattg gcaaccacta
    14641 ccaaagccat ataggtaata agaaagacca gtacggctaa gaattgacca ccttgtgtca
    14701 gaaatgcgag taaagtagca atagtcgaag gcttccgaat ctgaacctcg tcaaatccaa
    14761 gcgaaacaag aagagaccga agctcctcta ctgtcgcttt cccttggaaa ataaaatatt
    14821 tggttaaaag cgcagactcc accttttctg atgcctgaaa ttcctccagt cctcttggta
    14881 aggaagactg actaaaatta tcataagaaa aggtacgtcc acctgatgta cttggctcaa
    14941 tcacccgcct ggcaatcaca ctatccgtct gttgagaata ttcctctaat cgttgtctta
    15001 catctgagta agtcacgtcc tcagataccg acaagctggt cacagaagga taataatgaa
    15061 ctagcatagt ctccttttgg ctgacgccca accaaatcat aaagacggct agaatgatcg
    15121 tcgatacaag tacaaatttt cctttcattc taacttcacc tcattaacta tctgccattt
    15181 aatacactca actggatttt ttctgccttt ttcgcttgtt tgttcagtaa catccaacta
    15241 tttatcaaca gagccacaac tactcctagg ctaacaataa ctgactttgt cataaacatt
    15301 gccaaaccca tcccaacaag agcggagccg acttgttctc ctagataaga accatgcaat
    15361 tcgataaaac gcatccctgc tatccgcttg atggctattt ctcttttgaa ggtctcaaag
    15421 tatagaagat tcatcgtatt gaacaatagt atggaagtga atattccaag gattgtcgga
    15481 gatagggtca taagaatttc aagttgaaca ttttttctca gggtgttgta ctgttgcctg
    15541 ctagataaca agctacctat tgtgtttcgt aaattgtatt tctccaacag ctgttccagc
    15601 atctctttat ctttgaaaaa tacaaaatct ggtaaggcat tttcccaaaa ataggacgtt
    15661 tcttcaaaac tttcaggtga tagaacaaca atcaacggag cctgtaaaaa ttgctcataa
    15721 tttatcggag tatgattgta aatgaaccat cgcttctcgt ctgaaatgta ataaacgttt
    15781 gaatacaaac ttatggattt ttctccatcc gcaagcaatt tccctaccat gtcctcaaaa
    15841 tacgcttggt aaacactcgc atcattttgc aaactctttg gtaataataa tgcaatttct
    15901 cctttaccta attgctttat ctcttccagc tgattgaaat cgcacagtcc accttctgat
    15961 tgtatataat tcggcgtcac ataaagtacc ctttctagag gaatttcctt gctggattca
    16021 atgatggtat gttcttttat tgtgttatgc cgtaaatggt gctggactaa cattacatta
    16081 tttttcgcat atgattctaa aattacatga gaccaagcca ttctttgttg ctggaccatc
    16141 gcttcatcag ctatgaatgc ttcacgattc aaagacagac taaaaacatc ttgtctttct
    16201 tgccaagcct tttctcctat cgttagtgta tggagaactc caacgtatgt caacgttcga
    16261 ctagcaccaa tagagatgat gcaaaccgct aaacactgac ctactagtaa aatagcaaaa
    16321 atcgccttga gaggcaaagc tcccttaatt agcttttgta actgagaaat gtttaatgca
    16381 atcatgaatc caatacttgt cgataaagat aggaaaaaaa gcaaacaagc ataaaaggcg
    16441 ataccgattg ctacaaaacg ataatatacc attggccaac ccaaaattaa aatagcaatc
    16501 aatccaaaaa caagcgctcc tcctgtagac aatgctatat ctcttccatc cactcttaaa
    16561 aaacgactag caatctgcca actcctcacc cctgaaagca tctctatacc tgccgaccgc
    16621 aagtagacag tctgcccaac tactgtcaat gcaagattgg tgagtataaa gataataagg
    16681 cctaagctcg gagctcccct accaagaaat agacttaaac tgtttaatag ggtcggtcta
    16741 taaatatagc tatccaccaa tcccagtgac tgtaagacgt tttctaccac tttgatgtcc
    16801 agattccctt ttacaacata gtaattttca gctaagttgg cctgactagc tactaaccta
    16861 tccgctttga ccaagccctg tggaagctcc cccttcccaa aaacatcata actggtagta
    16921 gttcgcccac tatcatctgt ttctgttatt gtcttagcaa ttagactatt ttccttttca
    16981 gctaaatttg tcagggcatc cgacacttct tcataggtca cttcttcccc tcgacttaat
    17041 atggaaaccg atggatagta atgataaatt aaagaatcga gttgtagact cactaaccat
    17101 atacagaaga gacagacgct aaaagtagat aaataagtaa atactttttt cataacgaac
    17161 ctcatttgca caaaaaggag gtggactcca ccacctccct atcattagaa cccgtaacca
    17221 aaatcaacat gttttccaaa gtttattgta tcacttcgct attatctcta tcttaacata
    17281 taaacaaaac aaaaacaagg ttgcttgcat tcttaccatg ccgtacgcat gaagaaaatg
    17341 cgtataacca tttcctagac atctgcattt tcaaagcgtt ttcatgaaac caatacttca
    17401 accaggatcg tctttcattt ccaaagctat aaacagtttt ctaccaaaaa cggatgccaa
    17461 cagcaagaaa atagttttga aaacgaaaat ttctttattc gcttgttatg cgtaactata
    17521 tctcagctta actttgggtg attgtatcat acaagctgct gtgtttatcc acggcttctt
    17581 caatccctgt acgtacactc cctttgatac tggtttccaa ccgtcctctc atcgtttgaa
    17641 tcgtttgctt cgtttcgctg gctatcttct gaatgtctgt cttccctgtt ctttttatag
    17701 tgtcgcgttt atccaacaag actctagcct gcattactat gcgatagcgc tctattttcc
    17761 gccgtagttc tcggaaagag aaatcaggat ctatatcggt atagtaatca ttcaagtcca
    17821 tttgaatagt ccctccttaa ttggctaata gtgtcttcta agcggtagag gtctgtccga
    17881 tattgctggg acagcctctc ctcctgttga aacagttgat ccatctcttg acggtactga
    17941 taaaaggatt cttcttgtaa ttgatccaac tcttgaaaaa aggacaaggc cgcatcataa
    18001 tccaaggaat gacgactgag aaagcaacgc atctcttcct gttctctttc tatcaagagt
    18061 tttaaagcat tctgacgctg ttctaggtct tcttgacgtg cttcaatctt atttttctct
    18121 tggttatagt catcctccag atcaaataat tcttgctctc gcttacggat tctttcccat
    18181 attacatctg attccatttc tcaaaatctc ctgctagtct cttatctgtt tccagcattg
    18241 tgttgattgc ttgaataatt tgagtgtcca agatggtgaa cttttctgta gctatctctg
    18301 ccctctgccg atatatatcc atagtctctt caatctctct gacaatggtt tcctgactaa
    18361 caccatactc tgagaaaagg gcacaaactt cccaatagct gagagctgaa taggaatgaa
    18421 aatcaatcga attccacaac tcctctgctt ggtgtacata gtcatcgcaa tgggcaaaaa
    18481 tactcatttg ccctgcatct gcaacactag caatccctgc tgagatggtt ctggcctgct
    18541 cactgtccaa aaaaatctcc tcactacgac ttaggccacc tttagataag gctgttttct
    18601 gctttttcca gcgtttcata gccaaacgag tatcttccaa aggagcacca gtaaaggttg
    18661 ataccacctg accgttggca tcaatcagct gaccatattt gttaaacttc caagcattta
    18721 gcatgtgatc agctatagca tcccctggga tattcttcac cgctggataa atagctgatt
    18781 tcgtcttatt tcccgtaata tctccaagga agtcatactt atttcgatag ttacgaaact
    18841 gtgctctatg ctgctgcata tactctattt catcttctga aatcatattg tgaatgtcgg
    18901 gaccgttaaa ggtaatagca ggaagatctt tcttcaaagc aacatacatc gccagagagc
    18961 ctcctaacga atgccctgtg attgtgatgg ttgcatcggg atagcgttgt actatctgat
    19021 ctgcaaaagc taccgcagtt atagactgag atatataggg gacattctga ccgggaacgg
    19081 taaaagtagc cgtattccct ccgcctatca tagaaatatc tgttgcccaa tctttaatgt
    19141 cgccatcatt agtccctgcg taggcaataa cgacttggga agtatctgga atcggtttgt
    19201 tgtcagtgtc gtaacccttg atcggagcaa ccgccatggc ctgcataccg ttgcccgcat
    19261 caacaggtgg ctcgatgatg ataaaactac catttacaac tggagtatct tgtattagtg
    19321 gagttttagg tgcctttaat aggtctacat cataaaccac tttagaaata tctctataat
    19381 ctttatccga tatcatggca actccttata gtattttact tgtatattac taatttctgt
    19441 tactgtggtt gctactttag gaactaagta atctcctgtt tgagattttc caccatttat
    19501 tgtaatatca tcttctcccc aagatacaaa agtaacatgg aatttgtcat ttataattgc
    19561 atcactatcc catgtccctg tcttattatc cttttcaaaa tttacaaact ctatcttctc
    19621 aatcccttca tagtgattga cggcgtacaa aaccatccgc tcttgttcct ttcggatttg
    19681 agcctgcaat ttttcttcct cactcatacg gtgaaaacta aacattatca ttcctcctat
    19741 tgcgatacaa atggcactca taaccagtat gaggcgggga cattttttgg acatataaaa
    19801 cctccttaag ttgattatac tataaatact cggtatttac actagaatca ggataaggaa
    19861 aactggcaat aacgacttgg gaagtatctg ggatcggttt gttgtcagag tcatagccct
    19921 taatcggagc aaccgccatg gcctgcatac cgttgcccgc atcaacgggt ggctcgatga
    19981 tgacatactg cccttgtcct gctctaccgc cttgttttaa agtgtcatct gttttcaacg
    20041 gatctacttt atatacatca tctgcaatcc agcgataatc ctgatctgtt atcattagtt
    20101 atccccctta aaatagatta cctcaatgtg atttggattt gaaatggttg tcggcttgtc
    20161 tttaattcgt aaatacaaac ctgttttgtc tgaaggacca tcattcatcg acaaatctcc
    20221 atcaaatccc attgctgtta tcgtaacttt ataatcatca tttaatatag cacctgcata
    20281 ccaagcacct gtcatcctat tttctctaaa actggtaaac tcgatcttct caatcccttc
    20341 atagtgattg acggcataca agaccatacg ctcctgttcc ttacggattt gagcctgcaa
    20401 cttctcttcc tcactcatgc gatgaaaact aaacattatc attcctccta ttgcgataca
    20461 aatggcactc ataaccagta tgaggcgggt acgttttggg gacatataaa acctccttat
    20521 gttgattata ctataaatac tcggtattta cactagaatc aagataagaa aactatctta
    20581 tactaggcaa taacgacttg ggaagtgtct ggaatcggtt tgttttcagc atcgtagccc
    20641 ttaatcggag caaccgccat agcctgcata ccattgatca tatcttgggg ctgggagagg
    20701 atgacgaatt tatcttgtgc gactcggtcc ccatccttaa aagtgtcatc tgttttcaac
    20761 gggtctactt tatatacatc ctcagcaatc cagcgataat cctgaactgt tatcattggt
    20821 tatccccctc aaaatagatt acctcaattt gatttggaat tgaaatggtt gtcggcttgt
    20881 ccttaattcg taaatacaaa cctgttttgt ctgaaggacc atttgtcatc gacaaatctc
    20941 cgtcaaatcc cattgctgtt atcgtaactt tataatcgtc atttaatata gcacctgcag
    21001 accaagatcc tgtcatctta ttttcttcaa agctggtaaa ctcgatcttc tcaatccctt
    21061 catagtgatt gacggcatac aagaccatcc gctcttgttc ctttcggatt tgagcctgca
    21121 acttttcttc ctcactcatg cggtgaaaat taaacatcat cattcctcct attgacagga
    21181 acaacaaacc ggtgaataaa actagtcgtt ttttgcatac aaacataaaa atcctcctaa
    21241 tataattata ttattcttga acataacttt tcaaataaaa aggataataa agagcttaag
    21301 ttcaatacct taagctcttt aatctattta ctttgaagtg tttccctgtc attctgacag
    21361 caaatccaaa aacaagccat atcctgctgc ctccatttcc tctctcggta tgaagcgaag
    21421 ggctgcggag ttgatacagt aacggagacc gcctttgtca cgtggtccgt catcaaagac
    21481 atgacctaaa tgagcatgac cagcccgact gcggacttca atacggttca tgccgtggga
    21541 cttatcttgg aaataagtcg ccacttcctt gctgattggt ttggtaaagg agggccagcc
    21601 gcagcctgaa tcgaatttgt ctgtcgagag aaagaggggc tcaccagtcg taatatcgac
    21661 gtagatgcca gcttggtcgc tattccaaaa ctcattttcg aagggtcttt ccgtcgcagc
    21721 ttcttgagtg acttgatact ggaggtcggt caagctgtct ttcaagactt gctggtctgg
    21781 cttttggtag tctgcaacat caatcagagg gatttttgcc tgcccaagat cgatatggca
    21841 gtacccttgg ggatttttct tgagataatc ctgatggtaa tcttcagcgg ggatgtaatg
    21901 tgcgagtggc tccacttcaa cggctagggg gcgtccgcca aagagctggg actgctccat
    21961 catgacctgc tcgatggtcg gcaaatcagc cttatctgtg taatagatac cagtccgata
    22021 ctggcgacct ttgtcaggtc cctgctgatt gacagagaag ggatcaatga cgcggaaata
    22081 atagaggaga atttcccgca aattgatacg agtctcatca taggctagat agagtgtctc
    22141 tgcgtggtct gtttgtttga ggagttgata attggtcgtc tccacctgtc cattggcata
    22201 gccaacgctg gtgtcaagaa taccatcgat ctgggaaaag tagccttcca tcccccagaa
    22261 acaaccgcct gctaggtaga tttctttcat gtgaactcct tcttatctgc aagcgtggtt
    22321 tcgtcaccct tacagatttt tttctttcat ttctgcttcc attttgaggc gttgaacctt
    22381 catggtcgct gttcgtggaa tatcgtcata agccatcaag ataggctctt tcaatagtgg
    22441 taggtctgca accatggccc accaagcttc ccaattcatc tcagcatctt ctaccagagc
    22501 gataatcggc tgcggtgcac cattgacatc gcggataatc acgacttctg ataaaaagtc
    22561 caatttatcc agcaataagt cttccaaggc aaggttgctg tcgatgtgct caatgaggtc
    22621 aacctgacgg tcttttagaa gaagcgtccc ctctggagtc atgcagccgt agtcgccact
    22681 atcccaccaa gcaccgtaca cattatcttg gaagcgggca tcttctttgt agtaagtcac
    22741 agcacggcct tttgacagga actggatatg tccgttttct ccagctggga gaggattccc
    22801 ttgggcatct gtgatgcgtg cttctgtgta accttccaga ccaatcccca tatctcgtcc
    22861 gctaactgtt cctaggtttt ccaagcgatg gtaacgtaaa atcattggac cacattcact
    22921 ttgaccataa acctgcataa agactggatg ctgctcttta gaagcctcca agaaggcgcg
    22981 aagcgtacca atattgatag catcaaaggt cgaatggtaa taacgaatgc tactaaagac
    23041 ctctggtttt tccttggcca atcttgccca ttggacaaag ttattcggat gggtttccag
    23101 agcacttgga cggtgaagag caagcgcccg ttcaacatca tcttttcttg cagaggaaag
    23161 cgggtagagg gggaatccta gcccaatcgc cgacgacacg ccgatattat agcgtgaatg
    23221 tacaggggag atatggaagg ccaacaagcc tctctccgtc atcttgtcaa aaatagtttg
    23281 ttgccaagct actcgccagc ccatggtctg tgctgtgtgg cagattagtt ttggaacacc
    23341 tgtcgtccct gaagtatgag tcatgtattg aataacatct tctggcagga gattttcttc
    23401 aaaagcaact gcttcttgca ctaggacttg gggtaaaaat acctttgtta ccaggtcagt
    23461 tcgagtcatt cctgctacac gttcttccgt ttcttggtcg tagacaatga aagacttttc
    23521 caatcgctcc gcaaacacat ctaaattcga agatggcaaa tggtaggaaa tcataacagg
    23581 aacagcaccc aatgccgtta ccgctactgc taacaaatag gtgtcgaaag ctggactctt
    23641 atatagaatt accttgtccc catagccaat acctgctgct gaaaattggg cagctctttt
    23701 ttgaatagct tcaaaaactg tttgataggt attttccaat cctaattcag ggaaagatgc
    23761 gtagggtttg tcaaaataaa tggcaacatt tggaaactct tgagctgctg ttttaaactg
    23821 acgatagaga tttaatggtt tgtaactaat agttgacata tctaaccttt ctaaaatgac
    23881 aatgataagg ttattataac atcttgtgcc aaccttcaca atctgatatc tttttatgac
    23941 tcacagttgt gaaaaaacaa gtagtcaatc accacttgct ttcattttca attaaccaat
    24001 ctctgccaag atagctttga tttgattttt cgtgtggaca ccagcaactt gtttcacaac
    24061 ctgtccatct tttttgaaca aaagagttgg gatagacatg ataccaaact gacgagctgt
    24121 gtttggattc tcatcaacat ccatcttgta aatacgcaat tcatcttcgt ccacttcgtc
    24181 tgctaattgt tccaagatcg gtgcttgcat acggcatgga ccacaccatg gtgcccaaaa
    24241 atctacaagg actacgcctt cttgtgtttc aacttcaaag tttgcatctg taataacttg
    24301 aaccataatt ttctccgttt ctgtttttct actatcattc tacaccaaaa aaaaacagaa
    24361 agcgaatttt tgctacggga tgaagcatga gataaacgta tctataagaa tttcagttgt
    24421 aaaaaaatgg gtatagctaa actagaccgc aggaggacga gggggaactg ggagactgtt
    24481 cttcctataa aatccagttt tagcaatggg aaaagattta cgaaaggatg ctacgggatg
    24541 agatattact ctacctcttc caattttgta tattcttttg ccttttcttt cacaacttgt
    24601 tgtacctttt ttgaaaactc gccttttata cttgtatcaa aactgtttgc aatcaatttt
    24661 acggaatttt ctccatcatt tccaattgtt tgaattttct tcttgtgttg ttttaaaatt
    24721 gcattttttt ctcgcagttt gagtggagca tagatagagc gaatcagcca acgttcaatt
    24781 tctttctcta tagcattaat cgtcgcatag tctatctggt cagctttggt atataaatct
    24841 tgcaacattt gataaagttg atgcattggc tctcccttct attctcggta ttttgtataa
    24901 atttcgtcaa tctgatcttg catttttcgg tactggtttt tatagtaggt atccaagtcc
    24961 tcttgtttct gatccaatcg gtgaatggag ctgtatcgct cttgatcgat tgcggtatgc
    25021 cattcggaca tgccattgtt gagtaaatgg tgcaagtctc cagtctcgtc tccgacttgt
    25081 cgcaattcta acagaacttc ttgatacttt tcttccaaca ttcgagtcaa tattcgagtg
    25141 gtttcttcaa cattttcata gtgttcttgt agttcttttt gagcttttcg ttgttgattc
    25201 tcaatatcct cccattccca ctctttcttc attaactctg cctttaattt atcgtactgg
    25261 tccattcttg tatcctctgt gctaatgtct ggtcttccgt tactttctgc tcgatccctg
    25321 cttggatttg actttctaag tccactaaat cctgcttgag ttgattggag cgagtgataa
    25381 acttctcgac ttgattggtg ctatctccta ccagactatg atagtcaaca cctgcactgc
    25441 tgtaggcctg tctcacttca tcaggactca aaatgaagcc aaaagggaca tttcctaagc
    25501 tactgtagac ttcatttact tctgtaaggg tggaagtggt ctctttttcg agtgtttggt
    25561 gagtggtttc cgcaacctta accagtcctt gagccaggat gcgggcttgt tcgctgtcca
    25621 gatagatggt ttcacgcgaa ctgaagccac cacgggatag ggttgctttt aaagtagcat
    25681 agtggtacat ttccgacgag acatggtgga gagcattgga ataatgttgt tctagttttg
    25741 aagaaccttc ctttactttt aggctgccgt cggcgttcca ttgataacca ccccacacgt
    25801 gttgatcaat aggattgaga ttagtcttgc tgtccacacg atagactatg ccgattgctc
    25861 cctcactcgt tcctgcagtt gatttatagc cgggagcatc tttccctgaa tagccaatag
    25921 gaacgaaatc cttttgatca atgtaattat gaatacggta tttcagagca ttcactctgg
    25981 cttttcctgc ttccgtcaaa gcaggataaa catttgggcc gttatagata taagccccag
    26041 caatgcggtc aatatctgaa acagcggcca gagcatattg agcattcatt gagcctaacg
    26101 agtgaccgta gactgtaatt tgagcattag ggtagtcctt cagaacctgg ttcagaattc
    26161 tggaagcaga ctgaagttgg ggcgtcactt ctgacttatt agatataata ttgtgcgtca
    26221 tcggcaaatc attatccatc caatccgctg cagcgccttc cttccctgga cctttagaac
    26281 cttggaacaa gactatcaca ttgccgtcag gctcttcaac aacataaccg tccaaacctg
    26341 ttgcatcttt gagtacctta cggactgtgc cgatggttag ttcctcttca aaaaaatttg
    26401 taattacaac ttcattgcct tttaaatatg gagtatattc ttcagatgcc acctgctgtc
    26461 tatcaaaatc agttaaatct ctatttgttt ttgaattatt catttgcctc ttcacctaat
    26521 aacctagtta aatcggatga ctttccacct cccaccagtt taacaatatc atcccttatt
    26581 ttttcgatcc tataatttat atagagagta tcatcattat tcaaatatac ggaaaaattt
    26641 atccctccca ttggattgcg acgaatagaa gcactatcaa tggagtagga ccttattatt
    26701 ccttcttcgg ttaacgcttt ggcatcataa gaaattaacc tctcttcaac cattttcctc
    26761 acttcctcac ccttcacaat ctctaccatc tggtcgtgct caattttttg catcagctta
    26821 aatcctccta ttcctattgc gatggcaatt aagatgccaa caagtatttt aacaactatt
    26881 ttcattccaa acctccttta tgaaatccta tcaagtaaga gcatttctta cctcctagta
    26941 tagcataaat gcgatgttta tatataaaga cgcgcaaatc tatgagagta tcgattcacc
    27001 ccagtcattc cacacatctg caggttttgt caacacttca tttgatcctg tcgagcctcg
    27061 aaagagtacc attacttccg taacctggtc ttcgggcagg tttggatctt tcacaacagc
    27121 ataaacctgt tctccattgc cattctcatt gtcgaagtcg agggtgacac gacctactat
    27181 gtcatcatat tgtgtaatta cagcatctcc cttacctaaa ttattgtatt ccgtcattgc
    27241 aatctgaaga ttatcttcat taacatattt tttttaccat gaactacttc cctaataaat
    27301 ctgccaattt tgatttgata acataacttc cagacttaaa ctttccatct tcattttgta
    27361 aaaggttaaa atgaaagcca aagtcttcat ctccatttac ataaactctt acaatcattc
    27421 ctcccatggg attatactct agtgtttcag tgtcaatttc ataagttttt ataattccat
    27481 cagccgttaa tgcttttggg tcattcgctt tcatgtgttt ttcgtaaaca gctctagcct
    27541 cctcgctagt agcgatagcg accattttct catgttcctt gtgttgcata tatacaaatc
    27601 ctcctattcc aatcccgatt actaacaaaa ttccgataat aactttcatt acttttttca
    27661 tcctagacct cctttatgaa atcctatcaa gtaagatgct ttcttacctc atagtataac
    27721 atagatgcag ctgaaactgt gttaccgtca ccgtcacaac actcgttgcc gtcaggctct
    27781 tcaacaacat aaccatccaa acctgttgca tctttgagta cctgacggac tgtgccgatg
    27841 tatctagggt gttctagggt cccgatattt acctcatctc caaatttgta agctgaatac
    27901 tctttgttgg caattttctg tcgatcacga tcagtatact tttcagtcat tgtttatttc
    27961 tccaactaat tttttcactt cagaagaata ccctcccccg gagtattcaa tttttcctgt
    28021 attagaatcc ttttctaatt cgaacaccac tcttaacttt ctatctgaat tcaagaaaac
    28081 attaaaactt atccctccca tcggactatg aatgatagaa gaatcatcga tttcatatgt
    28141 ttttatcagc ccatcctctg aaagagcttc attatctaga tatttaagtt gttcttctac
    28201 aatctccctc acttcctcac tcttcacaat ctccaccatc tggtcgtgct caattttttg
    28261 catcagctta attcct
//
LOCUS       DAZQCJ010000018        28001 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000018 DAZQCJ010000000
VERSION     DAZQCJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28001
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.018"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(195..2189)
                     /gene="pknB"
                     /locus_tag="ACHU66_000707"
     CDS             complement(195..2189)
                     /gene="pknB"
                     /locus_tag="ACHU66_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFR3389010.1"
                     /translation="MIQIGKIFAGRYRIVRQIGRGGMADVYLARDLILDGEEVAVKVL
                     RTNYQTDQIAIQRFQREARAMAELDHPNIVRISDIGEEDGQQYLAMEYVNGLDLKRYI
                     KENAPLSNDVAVRIMGQILLAMRMAHTRGIVHRDLKPQNVLLTSNGVAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIILFEMLTGRIPYDGDSAV
                     TIALQHFQKPLPSVREENANVPQALENVVLKATAKKLNERYKSVAEMYADLASALSMD
                     RQNEPRVELEGNKVDTKTLPKLSQANVETKVPHTNSSAQVSATDKGSGKKEVAKSGNK
                     PVSKPRPGIRTRYKVLIGAILLTVIAAGLMFFNAPRTVTVPDVSGQTVEKATEMIEVA
                     GLEVGNITEEATATVDEGLVIRTSPAAKTTRRQGSKIDIVVATAALASIPDVVDKESD
                     TARQELEALGFQVTIKEEYSEKVAQGLVIKTDPGANSSAEKGAKITLYVSKGVAPQVV
                     PNVVGKSQENATQILQTAGFSIGTITQEYSSSVTAGQVISTDPVANTELAKGSIINLV
                     ISKGKELIMPDLTSGNYTYSQARSQLQALGVNAESIEKQEDRSYYSTTSDIVIGQYPA
                     AGATIDGTVTLYVSVASTRTSSDSSAGSSTSTSTSTGSGQ"
     gene            complement(2189..2926)
                     /locus_tag="ACHU66_000708"
     CDS             complement(2189..2926)
                     /locus_tag="ACHU66_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFR3389011.1"
                     /translation="MEIALLTDVGQKRSNNQDYINRYKNRAGIDLVVLADGMGGHRAG
                     HIASEMAATDLGAAWVDTQLVTLNDVREWMVAIIDAENQKIHELGQTEEYKGMGTTLE
                     AVVVIDNQMIYAHVGDSRVGLIRDGEYTRLTNDHSLVGALVRAGQLTEEEAQRHPQKN
                     IVTQSIGQAEPIEPDIALKTLEVGDYVLINSDGLTNMIPTEDIRDIVLSDVSIESKVE
                     TLVRFANNAGGLDNITVGLLHITEEAR"
     gene            complement(2964..4277)
                     /gene="rsmB"
                     /locus_tag="ACHU66_000709"
     CDS             complement(2964..4277)
                     /gene="rsmB"
                     /locus_tag="ACHU66_000709"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR3389012.1"
                     /translation="MVINKHETARSLALSVLEQVFDQGAYSNIALNKALESSRLSAQD
                     KGLATELVYGTVSRKITLEWYLAHFIEDREKLDTWVYYLLMLSLYQLVYLDKLPTHAV
                     VNEAVNIAKRKPGTDKFVNAILRKLSQDSLPKPADIKRKNKRLSVQYSVPVWLVQTLI
                     AEYGDERAEKIFQSLHDRNKASVRVTDGQQVEQLAEELGAQHSQLSPVGLVKSHGHFA
                     GTDYFKKGLITIQDETSQLVAPTLGIQGDEIILDACAAPGGKTCHMASYLTDGKVYAL
                     DLYDHKLNLIEENARRLGLEDKIETKGLDASRVHEEFGPDTFDKILVDAPCSGIGLIR
                     RKPDIRYNKASMDFDSLKTIQLQILDSVCQSLKIGGIITYSTCTIIAKENQEVIQEFL
                     QNHPNFEQVTLEHPQTDIMVDGCLLITPEQYKTNGFFIGQLRRKS"
     gene            complement(4264..5202)
                     /gene="fmt"
                     /locus_tag="ACHU66_000710"
     CDS             complement(4264..5202)
                     /gene="fmt"
                     /locus_tag="ACHU66_000710"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR3389013.1"
                     /translation="MTKLIFMGTPAFSAMVLRGLLADGNYNILAVVTQPDRAVGRKKV
                     IQMTPVKEVALEYNLPVYQPEKLSGSQEMDELMNLGADGIVTAAFGQFLPTKLLNSVD
                     FAVNVHASLLPKYRGGAPIHYALINGDERAGVTIMEMVKEMDAGDMISSDSIAIEESD
                     NVGTLFEKLAVVGRDLLLQTLPAYIAGDLKPVAQNPEQVTFSPNIQPEEEVLDWNKTA
                     RQLFNHIRGMYPWPVAHTYWQGERFKIQEAVEAEGEGPVGRVIARSKKELIIATGQGA
                     LSLKTVQPAGKPKMTIADFLNGAGRDIAVGDQFGDQ"
     gene            complement(5333..7723)
                     /locus_tag="ACHU66_000711"
     CDS             complement(5333..7723)
                     /locus_tag="ACHU66_000711"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR3389014.1"
                     /translation="MLAQIIVDVPLMQTDQAYSYRVPAELEEGLKVGVRVHVPFGKGN
                     RLIQGIVVDLIDEDDENNLQDLKELAEVLDYSPVLNQEQFWLADQMRKSVFSYKIALL
                     KSMLPSLLNSTYDKLLRPGLGLEMAEQISLFGDKEQIRFSDLDVTEQGKIMRLAQSGK
                     ILVDYVAKDKKQIKTEKWYRVCLEKLREADISNRAKKRQQLREFLLEHSEDQLLSNLK
                     SAYSADTLRYFQEKGYIEVWEEEVSRTQGVFDKVEKTQALDLNPEQAIAVREIVASIG
                     QESQTFLLQGVTGSGKTEVYLQVIDRVLKMGKTAIMLVPEISLTPQMTNRFISRFGQQ
                     VAILHSGLSDGEKYDEWRKIEAGNAQVVVGARSAIFAPLTNLGVIIIDEEHEATYKQD
                     SNPRYHARDVAKLRADYNRATLVLGSATPSLETRARASRGVYGRLTLNQRANPLARIP
                     EVEVVDFRDYIGQQEASNFTPVLIEKIREKLARKEQVVLMLNRRGYSSFVMCRDCGSV
                     DQCPNCDISLTLHMDTKTMNCHYCGFQKGIPQTCPNCQSRSIRYYGTGTQKAYDELQE
                     LLPEARILRMDVDTTKKKGAHEDLLERFGRGEADILLGTQMIAKGLDFPNVTLVGVLN
                     ADTALNLPDFRSSERTFQLLTQVAGRAGRADKEGEVLIQTYNPNHYAIAFAKEQDYEG
                     FYRYEMGIRKNLGYPPYYFTVGLTFSHKSEEFVVKKAYETVAFLRQHLTDAIQILGPT
                     PKPIARTHNLYHYQIILKYRHEDRLEEVLNQILDWTQERENQDLRLIIDNEPQNMM"
     gene            complement(8067..8381)
                     /gene="rpoZ"
                     /locus_tag="ACHU66_000712"
     CDS             complement(8067..8381)
                     /gene="rpoZ"
                     /locus_tag="ACHU66_000712"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR3389015.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAKPTQEFTSV
                     KSTLRALEEIESGNVVIHPDPEAKREAVRRRAEEAKRLAEEEERKIKAQIAKEKEEGE
                     KI"
     gene            complement(8405..9031)
                     /gene="gmk"
                     /locus_tag="ACHU66_000713"
     CDS             complement(8405..9031)
                     /gene="gmk"
                     /locus_tag="ACHU66_000713"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR3389016.1"
                     /translation="MKERGLLIVFSGPSGVGKGTVRKEIFESSDNKFEYSVSMTTRPQ
                     RPGEVDGVDYFFRSREEFEDLIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDGVFIFLTPPDLEELQDRLIGRGTDSEEVIAQRIERAKEEIALM
                     REYDYAVVNDEVPLAAERVKRIIEAEHFRVDRVIGRYDEMIREQIAER"
     gene            complement(9179..10792)
                     /locus_tag="ACHU66_000714"
     CDS             complement(9179..10792)
                     /locus_tag="ACHU66_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFR3389017.1"
                     /translation="MEIITVVVTLVSALIGLVLGYFAISLKMKSAKETAELTLLNAEQ
                     EASNVRGRAEEQAEVILKTAERDRQTLKKELLLEAKGEARKYREEIAEEFKSERQELK
                     QIESRLTERATSLDRKDDNLTNKEKTLEQKEQSLTDKAKHIDEREQEVVKLEEQKALE
                     LERVAALSQEEAKEIILTDTRDKLTHEIATRIKEAEREVKERSDKMAKDLLAQAMQRI
                     SGEYVAEQTITSVHLPDDAMKGRIIGREGRNIRTFESLTGIDVIIDDTPEVVVLSGFD
                     PIRREIARMTMETLLQDGRIHPARIEELVEKHRVEMDNRIREYGEAAAYEVGAPNLHP
                     DLIKIMGRLHFRTSYGQNVLRHSIEVAKLAGVLAAELGENVNLARRAGFLHDVGKAID
                     REVDGSHVEIGTELAKKYKENPIVINAIASHHGDVEATSTIAVIVAAADALSAARPGS
                     RRESMEAYIKRLQDLEEIANSFEGIKQSYAIQAGREIRIIVHPNKVTDDQITILAHDV
                     REKIENNLDYPGNIKITVIRETRATDVAK"
     gene            10920..11402
                     /locus_tag="ACHU66_000715"
     CDS             10920..11402
                     /locus_tag="ACHU66_000715"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR3389018.1"
                     /translation="MKKEVTVESFELDHTIVKAPYIRLISEEVGPKGDIITNFDIRLI
                     QPNENAMDTAGLHTIEHLLAKLIRQRIDGLIDCSPFGCRTGFHMIMWGKQDSEKIAQV
                     IKSSLEEIAEGITWEDVPGTTIESCGNYKDHSLHSAKEWAKLILSQGISTDAFERKPI
                     "
     gene            complement(11620..13074)
                     /locus_tag="ACHU66_000716"
     CDS             complement(11620..13074)
                     /locus_tag="ACHU66_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFR3389019.1"
                     /translation="MGEKNSHHLPLDEEKVLDFEVAKDLTIEEAVKKHKEIEAGVTED
                     DGLLDRYIKQHRAEIESQKFETKINHLPLVEVADEEKNQGHESAEEVEANESSLTEVS
                     EEIAPIVEELSVAPMETLEETVIASTVAMEGLSSVADDSSLELEEDEAEDLDHSEGAD
                     RDQKKKFYFWSAVGLSMIGVMVTALIWMNSVNKSNTATSSSSTSTSQTSSTASSSTDA
                     NVTALEQLYNSFFTDSSLTKLKNSEFGKLAELKVLLEKLDKNSDSYTKAKEQYDHLEK
                     AIAAIQAINGQFDKEVVVNGEIDTTATVKSGESLSATTTGISAVDSLLASVVNFGRSQ
                     QEVASATVASEAAVTSSQGADETVSTGVPATTEVASTTVSGSTTDFGIAVPAGVVLQR
                     DRSRVPYNQAMIDDVNNEAWNFNPGILENIVTISQQRGYITGNQYILEKVNIINGNGY
                     YNMFKPDGTYLFSINCKTGYFVGNGAGHSDALDY"
     gene            complement(13084..14250)
                     /locus_tag="ACHU66_000717"
     CDS             complement(13084..14250)
                     /locus_tag="ACHU66_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFR3389020.1"
                     /translation="MKKQFELIATAAAGLEAVVGREIRNLGYECQVENGRVRFQGDVK
                     SIIETNIWLRSADRIKIIVGQFPAKTFEELFQGVFNLDWENYLPLGCKFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHFSRPEGVPLQEMGAEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRVEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAAMLAKN
                     IAPGLKRSFAFEEWPWVEDQLVAALRKEAQASIKTDLVLDIAGSDIDARMIEIAKKNA
                     FAAGVEQDIVFKQMRVQDLRSDKINGVIISNPPYGERLLDDEAIVTLYREMGQTFEPL
                     KTWSKFILTSDELFETRFGQQADKKRKLYNGTLKVDLYQFFGQRVKHQVQEVQG"
     gene            complement(14304..14668)
                     /gene="rnpB"
                     /locus_tag="ACHU66_000718"
     ncRNA           complement(14304..14668)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHU66_000718"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(14807..15142)
                     /gene="gpsB"
                     /locus_tag="ACHU66_000719"
     CDS             complement(14807..15142)
                     /gene="gpsB"
                     /locus_tag="ACHU66_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFR3389021.1"
                     /translation="MASIKFTTKDIFEQDFKIGFRGYDQDEVNDFLDDIMKDYDAYEA
                     IIKELKGEIARLKAQAANSPKTTLPTEESNDVLRTERPSSATNFDILRRLNRLEKEVF
                     GKQIVQDQE"
     gene            complement(15210..15749)
                     /locus_tag="ACHU66_000720"
     CDS             complement(15210..15749)
                     /locus_tag="ACHU66_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFR3389022.1"
                     /translation="MDTKSLLVTGYRHTDLGIFSEKDPRLHIIKSAIRRNFIRFLEEG
                     VSWFILTGQLGFEYWSLEVLEDLRAEGYQLSIATIFPFENHGEQWNEANQAKLARFKQ
                     VDFVKYAYPRYENPGQFRDYNQFLLDNTTGCYLFYDSENETNLKYLYHMVLKKEGYNR
                     KTLTFEELNEEAENFSNSE"
     gene            15815..16432
                     /gene="recU"
                     /locus_tag="ACHU66_000721"
     CDS             15815..16432
                     /gene="recU"
                     /locus_tag="ACHU66_000721"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFR3389023.1"
                     /translation="MVNYPHKVSKKINRTSPISSQRVNFANRGMSFEAAINDSNQYYL
                     AHDIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYSGVFKRHYIDFEAKET
                     RQKASMPMKNFHAHQIEHMKQVVKQGGICFVLLHFSTLKETYLLPATHLIEFYQVDMG
                     SKSMPLTFIRQYGFEIQMGRFPSIPYLEIVEKNLLGGESFENYNN"
     gene            16413..18587
                     /gene="pbp1a"
                     /locus_tag="ACHU66_000722"
     CDS             16413..18587
                     /gene="pbp1a"
                     /locus_tag="ACHU66_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1A"
                     /protein_id="HFR3389024.1"
                     /translation="MKTTTIKKALLITANILLAGAIIACLAGAALVVYYIQSAPELTE
                     ESLTATVSSKIYDKNGNLIADLGAEKRSSAKTEEIPTDLVNAIVAIEDQRFFNHRGVD
                     VIRIAGSLINNLSGGRLQGGSTLDQQFIKLTYFSTSVEDQNLKRKIQEAWLATQLERR
                     NTKQEILTYYINKVYMSNGNYGMKTAALAFYGKELKDLTLPQLALLAGMPQAPNQYDP
                     YTNPEDAKARRDLVLAEMLEEKYIDNPQYEQAVLTPVTDGLQPLSNAAAYPAYMDNYL
                     KQVVEEVEAKTGYNLLTTGMDVYTNVDPAAQQQLWNIYNTDMYVSYPDDLLQVASTVV
                     DVSNGKVVAQLGGRKQETNVSFGTNQSVETNRDFGSTMKPITDYAPAFENGIYTSTAD
                     LILDGPYNYPGTPTPVNNWDKQYYGNISVKTAIQYSRNVTAVKALEATGLENALKFLN
                     SVGINYPDIHYSNAISSNTSDTSSKYGASSEKMAAAYAAFANGGTYYAPQYVNKIVFS
                     DGTVTEYAPKGTKVMSAETAYMMTDMMKAVMSYGYGLNASVSGVPMAGKTGTSNYTDS
                     ETDTILASIPEANYSYMVVPDENFVGYSSQYAMAVWTGYTNRMTPILDNSMRIATDVY
                     HNMMLFMHSDYTATDWEMPSGLVRYGSNYYLRGSRSLSNAYNTYTSSSTSSSSSTETS
                     ETTEATTTSSETSSGTATETATSSTETATTGNNPATGQTDGQ"
     gene            18646..19359
                     /gene="rsmG"
                     /locus_tag="ACHU66_000723"
     CDS             18646..19359
                     /gene="rsmG"
                     /locus_tag="ACHU66_000723"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR3389025.1"
                     /translation="MKPEVFYKTLADQGIQLTDQQKHQFHRYFQLLVEWNEKINLTAI
                     TEESEVYLKHFYDSIAPILQGHIQNEPLRLLDIGAGAGFPSLPMKIIFPQLDVTIIDS
                     LNKRINFLHLLAEELELEGVHFYHGRAEDFAQDKNFRAQFDLVTARAVARMQILSELT
                     IPYLKLNGKLIALKASSAEDELTQAKNALNLLFAKVIENHDYTLPNGDPRTLTIVEKK
                     KETPNKFPRKAGLPNKRPL"
     gene            complement(19549..19752)
                     /locus_tag="ACHU66_000724"
     CDS             complement(19549..19752)
                     /locus_tag="ACHU66_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR3389026.1"
                     /translation="MVQGTVKWFNAEKGFGFIAQENGPDVFAHFSEIQSNSFKSLEEG
                     QKVTFEVEQGQRGLQATNITKIG"
     gene            complement(19912..20745)
                     /locus_tag="ACHU66_000725"
     CDS             complement(19912..20745)
                     /locus_tag="ACHU66_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFR3389027.1"
                     /translation="MSQQKLIGYHPGTGFIHSLSAVSKLLFFLIVSILAMITYDTRLI
                     LFIAVFSLALFKMSGIRYKEISLVLILTIIFAAMNALMVHLFAPRYGVELYGADTPLL
                     SGLGVYSLTSQQAFYLVNLLLKYFCTVPLAIIFLMTTHPSQFASSLNQIGVSYKVAYA
                     VSLTMRYIPDIQEEFYTIRMSQEARGLELSKKGKLMDRIKGNLSLVIPLIFSSLERID
                     TISTAMELRRFGKNKKRTWYTQQPLQRIDYAVLLFILALVVVTIYLFFINQGRFYNPW
                     R"
     gene            complement(20742..22415)
                     /locus_tag="ACHU66_000726"
     CDS             complement(20742..22415)
                     /locus_tag="ACHU66_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3389028.1"
                     /translation="MKKIIEFKDFTFKYQAQQEPTLKDIQLTIYQGEKVLIIGPSGSG
                     KSTIGQCLNGIIPNIYKGEHSGSLLLDGQEAFNLSVYDKSLLVSTVLQDTDGQFIGLS
                     VAEDLAFALENDMESQVVMKQKVGDWAERLSLTDLLAHRPQDLSGGQKQRVSLGGVLI
                     DESPILLFDEPLANLDPKSGQDTIDLIDRLHKQEGTTTIIIEHRLEDVLYRQVDRVVL
                     INDGRILFNGGLDDLLRTSLLQENGIREPLYLSTLRALGYPIAQAEHLSSVWEIDIEA
                     LNIASLPTLHFENGEKESLLEVKHLHFSYPNKQVLTDIQLTIHKGERLAIVGKNGAGK
                     STLAKVLCGFIQGEGEILWKGQSIKEDSVKERAERIGYVLQNPNQMISQTMIFDEVAL
                     GLRVRGVTEEEIEHRVHHILKVCGLYPFRNWPISALSYGQKKRVTIASILVLNPEIIL
                     LDEPTAGQDQRHYREMMEFLDQLNAQGHTIVMITHDMQLMLDYSDRAVVVVDGQIIED
                     ASPAEILSDDMVIERANLKETSIFHLAERLGVNPLELTTFYMQEERRGR"
     gene            complement(22522..22782)
                     /locus_tag="ACHU66_000727"
     CDS             complement(22522..22782)
                     /locus_tag="ACHU66_000727"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR3389029.1"
                     /translation="MTLPEGGNLILMQADSFAQRVPFQVVASGNEVLISLNVASRKEV
                     DTLIERVEANSGQITGCPTDARGFYGASFTDLDGHHFNVIVM"
     gene            complement(23102..24085)
                     /locus_tag="ACHU66_000728"
     CDS             complement(23102..24085)
                     /locus_tag="ACHU66_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR3389030.1"
                     /translation="MSKDIRVLLYYKYVPIENAKEYAAEHLAFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDYVHANPLFSDLWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDIDPLVTTGAYLSPKEFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVRGELWDGKMYVFDERIAVDVNHVNPVVVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEHKYVRGCSAECRAHERNRYISENGLTRQEWAERLEAIGETLTPANA"
     gene            complement(24296..25216)
                     /locus_tag="ACHU66_000729"
     CDS             complement(24296..25216)
                     /locus_tag="ACHU66_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389031.1"
                     /translation="MVWTEEKLRPIIHSILNPDEEVLSLYQSKTTKSIYGCILWNSYY
                     YKVFRVSNHPSQSTYKQPTFYDFHGEFYMKGAIRTYLYHTNSWYELSKQSYYLLLFFQ
                     KLWDEKQEVEASWQNGRMQIRLVVEEEGWQVHYRFPDNQAREVERLLASGMLVAIRSS
                     RNFIKIRTSRFVAPYIALIKQRQLYRKKPSKAMLQWEKYQSQLIEIQRTYRLVEENAP
                     KTFFGHWLNKVQLYLCSAIASYWEEVIKGVEWQEAREIKDQRKIEKPHNPIEDKWKEN
                     IARRHKRKVDQLIGHDTLEKLRQLKTELDD"
     gene            complement(25324..25869)
                     /locus_tag="ACHU66_000730"
     CDS             complement(25324..25869)
                     /locus_tag="ACHU66_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HFR3389032.1"
                     /translation="MKNNSIKTVVATGIGAALFVVIGHLINIPTFVPNTSIQLQYAVQ
                     SLLAVVFGPVVGFLVGFIGHTLKDSLTYGPWWSWILASGVFGLVVGLTKKRLRIQEGI
                     FEGKDILFFNLVQIAANVLAWGVIAPVLDILIYSEAANKVFAQGLVAGIANSITVAIA
                     GTLLLAVYAKSQTKTGSLSKD"
     gene            complement(25890..26738)
                     /locus_tag="ACHU66_000731"
     CDS             complement(25890..26738)
                     /locus_tag="ACHU66_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFR3389033.1"
                     /translation="MSNNLLVLQSDFGLVDGAVSAMIGVALQESRDLVVHHLTHDITP
                     YNIFEGSYRLFQTVEYWPEGTTFVSVVDPGVGSKRKSVVALTEQNHYIVTPDNGTLSF
                     IKKYVGIKAVREISEVANRRANTEHSYTFHGRDVYAYTGAKLASGHISFEEVGPELSV
                     DKIVEIPTVPTEVGSDYVKGAIDILDVRFGSLWTSITREEFYTLQPQFEDRFEVTIYN
                     NDMLVYQNQVTYGKSFADVRIGQPLIYINSLYRVGVAINQGSFAKAYNVGVGQNWHIE
                     IKRISN"
     gene            complement(27170..27886)
                     /locus_tag="ACHU66_000732"
     CDS             complement(27170..27886)
                     /locus_tag="ACHU66_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR3389034.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPETN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRSAFGKNGGNMGASGSVSFMFDKKGVVVFAGDDADAIFELLLEADVEVDD
                     VEAEDGTITVYTAPTDLHKAIVALKESGIQEFNVTELEMIPQSEVSLEGDDLATFEKL
                     YDALEDDEDVQKIYTNVDGF"
BASE COUNT         8037 a         6519 c         5055 g         8390 t
ORIGIN      
        1 tagaactgga gttcatcaag gcaagttgac aacggataat tttgattttc gaagagtata
       61 aatgagcttt aatttgatct agcatgactt ttcccctttt atttttagtg taacataaaa
      121 aaactaaccc atcacaaaat gtgataggtt agataactta atgacattgg gtatctcccc
      181 aaccttattc atctttattg tccgctacct gttgaagtac ttgtacttgt ggaacttcct
      241 gctgaagaat cgctactggt tctggttgat gcaacagaga catacaaggt aactgttcca
      301 tcaattgttg ctccagcagc aggatattgt ccaatgacga tgtcacttgt cgttgaataa
      361 tagcttctat cttcttgttt ttcaatactt tccgcattga cacccaaggc ttgtaattga
      421 ctacgcgcct gtgaataggt gtaattgcca gatgttaaat caggcataat caattctttt
      481 ccttttgaaa taactagatt gatgatagaa ccttttgcca actctgtatt ggcaacggga
      541 tctgtgctga taacctgacc tgcagtaaca gatgagctat attcctgagt aatggttcca
      601 atgctgaagc ctgctgtctg aagaatttga gtagcattct cctgactttt tccaacaacg
      661 tttggaacaa cttgtggcgc cactcctttg gaaacgtaaa gggtaatttt tgcacccttc
      721 tcagcagaac tatttgctcc tggatctgtt ttgataacga gtccttgggc aactttttcg
      781 ctatattctt cttttattgt cacttggaaa ccaagggcct ccaattcctg ccgcgcagta
      841 tcagactcct tatccaccac atctggaata gatgccagcg cagccgttgc tactacaata
      901 tcaatcttac tcccttgacg tctggtcgtc tttgcagccg gagaggtccg tattaccaag
      961 ccttcatcta cagtcgctgt cgcttcttct gtgatatttc caacttccaa tcctgccact
     1021 tcaatcattt ctgtagcttt ttcaacggtt tgaccagata catcagggac tgtaactgtt
     1081 cgtggagcgt tgaaaaacat gagtccagcc gctatgactg ttaacaaaat agctcctatc
     1141 aagactttgt agcgtgtacg tatgcctggc cttggttttg aaacaggctt atttcccgac
     1201 ttcgccactt ctttcttacc agacccctta tccgtcgcag aaacttgagc agaactgttt
     1261 gtatgcggta ctttcgtttc aacattggct tgggagagtt tcggcaaggt ttttgtatca
     1321 accttgttcc cttctagttc aaccctgggt tcattctgac gatccatgga tagagccgaa
     1381 gctaaatctg catacatttc tgcaaccgac ttatagcgct cgtttaattt ttttgccgtt
     1441 gcttttagta caacattttc taaggcctga ggtacgttgg cattctcttc tctaacagat
     1501 gggagaggtt tttggaaatg ctgaagagcg atcgtcacag cactgtctcc atcataggga
     1561 atgcgacccg tcaacatttc aaaaaggatg attcccatcg cataaatatc actttgaatg
     1621 gtcgctttcg aaccacgcgc ctgctcaggt gaaagatagt gaacagaacc caacatggag
     1681 ttggtttgtg tcaaactcgt ttcagcaaag gcaactgcaa taccaaagtc tgttacttta
     1741 gcgactccat tggaagtcaa aaggacgttc tgaggtttca aatctcgatg gacaattcct
     1801 ctcgtgtgcg ccatgcgcat agcaaggaga atctgtccca taattcgcac cgcaacatcg
     1861 ttggaaagtg gtgcattttc ttttatataa cgttttaaat cgaggccatt gacatattcc
     1921 atagccagat actgttgacc atcttcttca ccgatgtccg aaatacgaac gatattggga
     1981 tggtcaagtt ctgccatggc acgcgcttca cgttgaaaac gctggatagc aatttgatct
     2041 gtctgatagt tggtccgaag aactttaaca gcaacctctt ctccatctaa aatcaaatct
     2101 cttgctaggt aaacatctgc cataccgcct cgaccaattt gccgaacgat tcgataccga
     2161 ccggcaaaga tcttaccgat ttgaatcatt acctagcctc ctccgtaatg tgaagcaaac
     2221 ctactgtaat attatctaga ccgcctgcat tattggcaaa acgtacaagt gtttctacct
     2281 tgctttcaat tgaaacatca ctcaatacaa tatcacgaat gtcctcggtt ggtatcatat
     2341 tggttaagcc atcgctgtta ataaggacat aatctccaac ctcaagagtt ttcaaagcaa
     2401 tgtctggttc aattggttcg gcttgtccaa tggactgcgt tacaatattt ttttgaggat
     2461 gacgctgtgc ttcttcttct gtgagctgac cagctcgaac aagggcacct accagcgaat
     2521 ggtcgttagt tagacgtgta tattcgccat ctctaatcaa tccaactcgt gaatctccaa
     2581 catgggcata gatcatttga ttgtcgatga caacaactgc ctcaagagtt gttcccatcc
     2641 ccttgtattc ttctgtctgt ccaagttcat gaatcttttg gttttcagca tcaatgattg
     2701 caaccatcca ttcacggaca tcattcaaag tcactaattg agtatcaacc caagcagctc
     2761 ccaaatctgt tgctgccatc tcactagcaa tatggccggc acggtgacca cccattccat
     2821 cagctaacac gaccaaatca atccctgcgc ggtttttgta gcgattgata tagtcttggt
     2881 tgttcgaacg tttctgtcct acatcagtaa gtaatgcaat ttccataatt tttatcgttg
     2941 ctaagcaact tcctcctctt attctaggat tttcgtctaa gttgaccgat gaaaaagcca
     3001 tttgtcttat actgttcagg ggttatcaac aagcagccat ccaccataat atctgtttga
     3061 gggtgttcta gtgtcacctg ttcaaaattg ggatggtttt gcagaaattc ttgaataacc
     3121 tcttggtttt ccttagcaat aatcgtacat gtgctatagg ttattatacc accaattttc
     3181 aagctttggc aaacactgtc cagtatctgc aactggatag tctttagaga atcaaagtcc
     3241 atcgatgcct tgttataacg aatatctggc ttacggcgga tgagtccaat acctgaacaa
     3301 ggggcgtcca ctaggatttt atcaaaggta tctggaccaa attcctcatg aacacgactg
     3361 gcgtccagac ctttggtctc aatcttatct tctagaccta aacgacgtgc gttttcttca
     3421 atcaggttta gtttatgatc atataaatcc aacgcataaa cttttccatc cgtaagataa
     3481 cttgccatat gacatgtctt tccacccgga gctgcacagg catccaagat gatttcatct
     3541 ccttgaatac ccaaagtcgg agctaccaat tgactggttt catcttgaat ggtgataagt
     3601 cctttcttga aataatctgt ccccgcaaag tgtccatggg atttaaccaa cccaactggt
     3661 gataactgtg agtgttgggc gcctagctcc tctgccaatt gctccacttg ttgaccatct
     3721 gtcacgcgta cgctggcctt attccgatca tgtaagcttt ggaaaatctt ctctgcacgt
     3781 tcatcgccat attctgcaat cagggtttgg accaaccaaa ctggcacaga atactgaacg
     3841 gatagacgtt tgttctttct tttaatatct gctggtttcg gcaacgaatc ctgactaagc
     3901 ttacgcaaaa tagcgttgac aaacttatct gtcccaggct ttcgcttggc aatattaact
     3961 gcctcattga caactgcgtg ggtaggcaat ttgtccaaat ataccaactg gtagaggctc
     4021 aacatgagga gatagtagac ccaagtgtcc aacttttccc tatcttcgat aaaatgcgcc
     4081 aaataccact ctagggtaat tttacgagaa accgtcccat aaaccaattc ggtcgccaag
     4141 cccttgtcct gtgctgataa acgagaagat tctagagcct tattgagagc gatgttcgag
     4201 taagcacctt gatcaaagac ttgttccaat acagataagg ctagacttct tgctgtttca
     4261 tgtttattga tcaccaaatt gatctcctac tgcaatatct cgacctgcac catttaaaaa
     4321 gtctgcaatg gtcatttttg gcttaccagc tggctggaca gttttcagag aaagggctcc
     4381 ctgacctgtc gcaatgatca attctttctt gcttcgagca ataacccgac caactggtcc
     4441 ttcaccttct gcttctacag cttcttgaat tttaaaccgt tccccttgcc aataggtgtg
     4501 ggcaacgggc cacgggtaca taccgcgaat gtgattaaat agttgacgag ctgtcttatt
     4561 ccaatccagt acctcttctt ctggctggat atttggtgag aaggttacct gttctggatt
     4621 ttgagcgact ggtttcaagt caccagcaat ataggctggt aaggtttgta ataataaatc
     4681 ccgaccaaca acagccaact tttcaaacaa ggtgccgacg ttgtcgcttt cttcaatagc
     4741 aatgctatcg ctggaaatca tgtcaccggc atccatttcc ttgaccattt ccatgatggt
     4801 tacgccagca cgttcatcac cgttaatcaa agcgtaatgg ataggggcac cgccacggta
     4861 tttaggaaga agagaggcat gaacattgac tgcaaagtca actgagttca ataatttggt
     4921 tggtaggaat tgcccaaaag ctgccgtcac aatcccatct gctcctaaat tcatcagctc
     4981 atccatttct tgacttccag atagcttctc tggttgatag actggtaaat tgtattccaa
     5041 ggctacttct ttcacaggtg tcatttggat gacttttttt cgtccaacag ctcggtctgg
     5101 ctgggttaca actgccagta tgttataatt tccatcagct aagagccctc ttaagaccat
     5161 cgctgaaaac gctggcgttc ccataaaaat caactttgtc atattcgtgt ctcatctcct
     5221 tttcattttc tgattcatat caattatact atcccctaat tatatcattt ttgcttattt
     5281 tttccttaat caaaggttct taaaacaaaa aaatgcaagg tcaatagtga cctcacatca
     5341 tattctgcgg ttcattgtca ataatcagtc gcaaatcttg attttctctc tcctgtgtcc
     5401 aatccaaaat ctgattcaat acctcttcca aacggtcttc atgccggtat ttcaagatga
     5461 tctggtagtg gtagagatta tgagttctgg caataggctt gggcgtcggt cccaagattt
     5521 gaattgcatc tgttaagtgt tgacggagaa aggctacagt ttcataggct tttttcacga
     5581 caaattcttc tgacttgtgc gaaaaggtta agccaactgt aaaatagtaa ggtggataac
     5641 caagattttt ccgaatgccc atctcataac gatagaaacc ttcataatcc tgctctttgg
     5701 caaaagcaat ggcataatga ttggggttat aggtctgaat caggacctcc ccttccttat
     5761 cagctcgacc agcccgacca gctacctgag ttagcaactg gaaggtccgc tcagaagacc
     5821 gaaaatcagg caaattcaga gccgtatccg cattgagcac gccaactagg gtcacatttg
     5881 gaaaatccaa gcccttggca atcatctgag ttcccagaag aatatccgct tcgcctcgac
     5941 caaaccgttc gagcaagtcc tcatgagcac cttttttctt ggtagtatcc acatccatac
     6001 gcaaaattct agcttctggc aagagctcct gcaattcatc gtaggccttt tgcgtacccg
     6061 tcccataata acgaatagaa cgactttgac agtttggaca ggtctgagga atcccctttt
     6121 gaaaaccaca ataatggcaa ttcatcgttt ttgtatccat gtgaagcgta agggaaatat
     6181 cacagttggg acactggtca acactcccgc aatcacgaca catgacaaaa gaagaataac
     6241 cacgcctatt caacataaga acgacctgtt ccttacgagc caatttttct cttatctttt
     6301 caatcagaac aggggtaaaa ttactagcct cctgctgacc gatataatca cggaaatcca
     6361 caacttccac ttctggaata cgagccaagg gattggccct ctggttaaga gtcagtcttc
     6421 catagactcc tcgactagcc cgagcccgtg tttcaagact cggcgtagca gaacccaaga
     6481 ccagggtcgc tcgattataa tcagcccgca actttgccac atcacgcgca tggtagcgtg
     6541 gattgctgtc ctgcttgtag gtcgcctcat gctcctcatc aatgatgata acacccaagt
     6601 ttgtcaatgg tgcaaaaata gccgaacgag ccccgacaac cacctgggca ttccccgcct
     6661 caatcttccg ccactcgtca tacttctcac catcagacaa gcccgagtgt aaaatggcaa
     6721 cctgctggcc aaaacgagaa ataaaacgat tggtcatctg aggggtcaag gaaatttcag
     6781 gcactagcat aatagccgtt tttcccattt tcaagaccct atcaataacc tggagataga
     6841 cttccgtctt cccagaacct gtcactcctt gcagtagaaa tgtctgactt tcctgaccga
     6901 tagaggccac aatctcacga accgcaatcg cctgctctgg attcaaatcc aaggcctgcg
     6961 ttttttccac cttatcaaaa actccctgag tccgcgacac ttcctcctcc cagacttcta
     7021 tatagccttt ttcttggaaa tagcggagcg tatcagctga ataggccgat tttaggtttg
     7081 aaagaagctg gtcttccgag tgttccagaa gaaattccct taactgctgc cgtttcttgg
     7141 ctcgattgct gatgtctgct tctcgtaatt tctccagaca aactcggtac catttttctg
     7201 tcttgatttg cttcttatcc ttggccacat agtctaccaa gatttttcct gactgggcca
     7261 gacgcatgat tttcccttgc tctgtcacgt ccaagtcaga aaaacgaatc tgctccttat
     7321 caccaaacag actaatctgc tctgccattt ccaaaccaag ccctggacgg agcaatttgt
     7381 cataggtcga attgagcaag ctaggcaaca tggattttaa gagggcaatt ttataggaga
     7441 agaccgactt tctcatctgg tccgccaacc aaaattgctc ttgattgaga acaggactat
     7501 agtccaaaac ctcggcaagc tctttcaagt cttgtaagtt attttcatca tcttcatcaa
     7561 tcaaatcaac tacaatacct tgaatcagcc gatttccctt accaaagggt acatggacac
     7621 gcacgccaac cttgagcccc tcttccaact cagctggaac tcgataggaa taagcttgat
     7681 ctgtctgcat caaagggacg tctacaataa tctgtgctaa cataccatct ccatttattt
     7741 taccttctaa tatcacgact gatgcgaaca aggttcactt tatgtataac gaggctgggc
     7801 aaaaagccca ggaccactac tcagagttcg tgtcaacatc tcagcgcagt ggttgattgg
     7861 cagatttgtt cgtgttttgc actccaaatc tagcctaatc aactgtgcgg gggtgggaag
     7921 acgaactctt tttatcttgg tcgagttctt tcccactccc caaatattaa aagataaact
     7981 aaatgacctg cctcctttct gtcaaaactt tcatcattcc gaaagtgaaa agaaacccgg
     8041 atggcaatcc caaccgagtc ttgtttctaa atcttttcgc cttcttcttt ttctttagca
     8101 atttgagctt tgattttacg ctcttcttct tccgccaaac gtttagcctc ttcggcacgg
     8161 cgacgaacag cctcacgttt cgcttctggg tctgggtgaa tgactacatt acctgattca
     8221 atttcttcca aggcgcgtag ggtggatttc actgaggtga attcttgagt tggtttggca
     8281 cctgcttcca attcatgggc acgcttagcc tcaagaataa ccaaagaata cttagatggt
     8341 accttatcca acaaggtatc aattgacggc ttcaacatca taatgcgatt ctctcttctt
     8401 tcttttatcg ttctgctatc tgttcacgaa tcatttcatc gtagcgacca atcactcggt
     8461 ctacacggaa atgttctgct tcgataatac gcttgacacg ctcagcagcc aacgggactt
     8521 catcgttcac aactgcataa tcatactcac gcatcaaggc gatctcttcc ttggcacgtt
     8581 cgatacgttg agcaataact tcctcactat ccgttccgcg accaatcaag cgatcttgca
     8641 actcctccaa gtcaggtggt gtcaagaaaa tgaacacccc atctggaacc tttttcttca
     8701 cttggagggc accctgtact tcaatttcta aaaagacatc aattccctta tcaagagttt
     8761 cattgacata ggtcagaggt gttccatagt aatttccaac gtactctgca tattccaaca
     8821 tttgaccctg gcgaatcaaa tcttcaaatt cctcacgact acggaagaaa tagtcaactc
     8881 catcaacttc tcccggtctc tgtggacgtg tcgtcatgga tacagaatat tcaaatttat
     8941 tatcagagct ttcaaaaatt tcctttcgaa ctgttccttt tccaacacca gatgggccag
     9001 aaaagacaat gagtaagcct cgctctttca tgatatctcc ttttaaatct ttcattctag
     9061 tgtaacaaaa tttagcatga atttcaactg cttttaaata cgattagaat attttctatt
     9121 cccaacccta aacgaacttt gagccagtag aaaaagagcc cttcggactc ttctacattt
     9181 acttagcaac atctgtcgct cttgtttcac ggataactgt gattttgata tttcctggat
     9241 aatccagatt attttcaatt ttctcacgaa catcatgggc caagatggta atctgatcat
     9301 cagtgacctt attaggatga acgataatcc gaatttcacg accagcctga atagcatagg
     9361 attgtttaat cccttcaaaa ctgttggcga tttcttccaa gtcttgcaga cgtttgatgt
     9421 aggcttccat agattcacga cgcgaacctg gacgagcagc gctcagagca tctgccgcag
     9481 ccacaatgac tgcaatagta cttgtcgctt ctacatcgcc atggtgacta gcaatcgcat
     9541 tgataacaat tggattctct ttatatttct tagccaattc tgtaccgatt tcaacgtggc
     9601 ttccatccac ctcacggtca atagctttac caacgtcatg taggaaacca gctcgccgtg
     9661 ccaaattcac attctcgccc aattcagcag caagtacacc tgctaattta gcaacttcga
     9721 ttgagtggcg aagaacattt tgtccataag aggtacggaa atgcaaacgt cccatgattt
     9781 taatcaagtc tggatgaagg tttggtgccc caacttcata agcagcagct tccccatact
     9841 cacgaatacg gttatccatc tctacacggt gtttctcaac caactcctca atacgagctg
     9901 gatgaatacg accatcttga agcaaggtct ccatggtcat acgggcaatc tcacggcgaa
     9961 tcggatcaaa acctgataga acaaccactt ctggtgtatc atcaatgata acatcgatac
    10021 ctgtgagact ttcaaaggta cgaatatttc gaccctcacg tccaataatc cgtcccttca
    10081 tcgcatcatc cggtaaatga accgatgtaa tcgtctgctc agccacatat tcacctgata
    10141 tgcgttgcat cgcttgggct aacaagtcct ttgccatctt gtcagaacgc tcttttactt
    10201 cacgctctgc ttccttgata cgagtcgcaa tttcatgcgt cagtttatcg cgtgtatcgg
    10261 taaggataat ttccttcgcc tcttcttgac tgagggccgc cactcgttct aactccaaag
    10321 ctttttgttc ttcgagtttt acaacttctt gttcacgctc atcaatgtgc ttagctttat
    10381 cggtcagact ttgttccttt tgttccaaag tcttttcctt gttggtcaaa ttatcgtctt
    10441 tgcggtccaa gctggtcgca cgctctgtca agcgtgattc gatttgcttc aactcttgac
    10501 gttctgactt aaattcttct gcaatttctt ctcggtactt acgagcttct cccttagctt
    10561 ccaagagtaa ttcttttttc aaggtctggc gatcgcgctc agctgttttt agaataacct
    10621 cagcttgctc ttctgctcga ccacgaacat tactagcttc ttgttctgcg tttaaaagtg
    10681 tcaattctgc agtttctttt gcagatttca tcttcaaaga aatggcaaag taacctaata
    10741 ctaaaccaat gagagcagaa acaagagtca ctacaactgt aatgatttcc atattctacc
    10801 tcttttttat aattcgattt tcacaaaatc tgtatctatt ttatcatatt tgaaacgatt
    10861 tcacaattcc aaattgtcaa atatggtata ataaattcat cacttatttg gaggaaacca
    10921 tgaaaaaaga agtcactgtc gaaagcttcg agctagacca cactattgta aaagcacctt
    10981 acatccgctt aatttctgaa gaagtcggac caaaaggaga cattattaca aactttgata
    11041 ttcgccttat ccaacccaac gaaaatgcaa tggatacagc tggtctacat accattgagc
    11101 atctattagc taaactaatt cgccagcgca tcgatgggct aatcgactgc tctcctttcg
    11161 ggtgtcggac aggtttccac atgattatgt ggggaaaaca agactctgaa aagattgctc
    11221 aagtcatcaa gtcgagtttg gaagaaattg cagaaggcat cacttgggag gatgtcccag
    11281 gaacaaccat tgaatcatgc ggtaactaca aagaccacag ccttcattca gctaaagaat
    11341 gggccaaact aatcctttct cagggaatct ctacagatgc ctttgaaaga aaaccaatct
    11401 aacaaaaaaa cggatacacc gctcagaacg tagacaaaat cctaaaatat aaaaaattga
    11461 agaagtttac gaaagatagc aagtcattaa actcttagaa acattgatat ttcggcgttt
    11521 ctgagagttt tttattttca acataaagaa aaaagattga agaaaattgt ccaaaatgga
    11581 ctttttcttc aatctgaagc ggctaaaacg cttttttgct taataatcca aggcatcaga
    11641 atgaccagca ccattgccga caaagtagcc tgtcttacaa tttatagaga acaagtaggt
    11701 cccatctggt ttaaacatat tgtagtagcc attgccgttg ataatattaa ctttttccaa
    11761 gatatattga ttgcctgtta tatacccacg ttgttgcgag atagtcacaa tattttcaag
    11821 aatacctgga ttgaaattcc atgcttcatt gtttacatca tcaatcattg cttgattata
    11881 aggaacacga ctgcggtcac gttgcaagac gacaccagct ggcacggcaa taccaaagtc
    11941 ggttgttgag cctgaaaccg ttgttgaagc gacttctgtt gtcgctggta caccagtaga
    12001 tacagtttca tcagcacctt ggctgcttgt gactgcagcc tcacttgcta ccgtagccga
    12061 agcgacttcc tgttgtgaac gaccaaaatt cacgaccgat gccaatagac tgtcaaccgc
    12121 actaatccct gtcgttgtcg cagaaagaga ttctcctgat ttaaccgtcg ccgttgtatc
    12181 aatttcacca ttaacaacga cttctttatc aaactgacca ttaatggcct gaatggctgc
    12241 aatggctttt tcaagatgat catactgttc tttagctttg gtataactgt ccgaattctt
    12301 atccaatttt tccaacagca ctttcagttc tgctaattta ccaaactctg aattttttag
    12361 cttggtcaaa ctactatccg taaagaaact attatagagc tgttctaaag cggtcacatt
    12421 cgcatctgtc gaacttgatg ctgtcgatga cgtctggctt gtacttgttg aacttgaaga
    12481 agtcgcagta ttcgatttgt taaccgaatt catccaaatc agcgccgtca ccattacacc
    12541 aatcattgaa agccctactg ctgaccagaa gtaaaatttc tttttctggt ccctatctgc
    12601 gccctcacta tggtccaagt cttcggcttc atcttcttcc aattctaaac tagaatcatc
    12661 tgccactgaa gataacccct ccattgctac agtacttgca atcactgttt cttccaaggt
    12721 ttccatcgga gctaccgaaa gttcttctac aataggagca atctcctcac taacttccgt
    12781 aagagaagac tcatttgctt ctacctcttc cgcgctttca tgtccttgat ttttttcctc
    12841 atcagccacc tcaactaaag gaagatgatt gattttggtt tcaaattttt gagattcaat
    12901 ttctgcacgg tgttgcttga tgtaacgatc caagagacca tcatcttctg tcacacccgc
    12961 ttcaatttcc ttatgttttt tgactgcttc ttcgattgtt aaatctttcg ccacttcaaa
    13021 atctaaaact ttctcctcat ccaaaggaag atgatgacta tttttttctc ccacgatgtc
    13081 ctcctatcct tgcacttctt gcacttgatg tttcactcgt tgaccaaaaa attgataaag
    13141 atccactttt agggtaccat tatacagttt gcgtttctta tccgcttgct gtccaaaacg
    13201 ggtttcaaac aactcatcac tagttagaat aaacttagac catgttttga gtggttcaaa
    13261 agtctgcccc atttctcgat aaagggtaac aatggcttca tcatccaaca aacgttctcc
    13321 gtaaggtgga ttagaaatga tgacaccatt aattttatct gaacgcaagt cttgcacacg
    13381 catttgcttg aaaacaatat cctgctcaac tcctgccgca aatgcattct tcttcgcaat
    13441 ttcaatcata cgagcatcaa tatctgatcc agcaatatct aataccaaat ctgttttgat
    13501 agaagcttgc gcttctttgc gtaatgcagc caccaattga tcctcaaccc acggccattc
    13561 ctcaaatgca aaacttctct tcaaacctgg agctatattt tttgccaaca tagctgcctc
    13621 aatacagaaa gttcccgatc cgcaagtagg gtcaataagc ggtttatctg gataccaatt
    13681 cgataattgc aaaatagcag ccgccatatt ttctttgatt ggtgcgccac ctttttccac
    13741 ccgataacca cgtttgaaca aactagaacc tgtcgtatca atcatgactg tcgccacatc
    13801 tttcaaaatc gacacttcaa ttttaaactc tgcccccatc tcttgcaagg gaaccccttc
    13861 gggacgagag aagtgtttct gcagtttctt tacaactgct ttcttagaaa tagcctgcac
    13921 acttggttca ttatgcaatt tcgatttgac acatttggcc ttggaaatgg ggaacttgca
    13981 gcctagaggc aagtaatttt cccaatccaa gttaaaaact ccttggaaca actcttcaaa
    14041 agtctttgct ggaaattgtc caacaatgat tttaattcta tctgctgaac gcagccagat
    14101 attggtttcg ataatggatt ttacatctcc ttggaatcta acacggccat tttccacttg
    14161 gcattcatag ccgagattac gaatttctcg acccacaacc gcttccagac cagctgctgc
    14221 cgttgcaatc aactcaaatt gttttttcat ttttttcctc tacaataaaa gctagcagag
    14281 ctagctacct attttatgca attttctgta agccatgttt tgttccagtg gtactaggtt
    14341 ctaccacctt cgataatcat ctgtctactg tctaaacagt caggatttct gttcatttcc
    14401 atccatccca tgccccgacc aaagtttggg ttgctagctt gaggggttta ccgcgttcca
    14461 ttttttacgt ttccataaaa acttcgtcac tgtggcactt tcagatctac tctgacatat
    14521 caaaagactt agccatttta accgccgtaa cgcccaaggg cgtccctagg cttattgatt
    14581 cgcctagcac aaacactact ggcatctcag ccagtgctag catggacttt cctcatggta
    14641 gaaaactacc acgcgattat ccaaaaattg caccttaatt atcttagaag ttgtatctac
    14701 aaagcaagcc actttaaatc aaggaatatc tctgacacaa atacagaatg tatttgtgca
    14761 caagaaaata actgcaaata aagtgtcagt ttaggtgtac aattttctat tcttgatcct
    14821 gaacgatttg ttttccaaat acttctttct ccagacgatt cagacggcgg agaatatcaa
    14881 agtttgtcgc tgaagatgga cgttctgtac gcaatacatc gttcgattct tcagtaggaa
    14941 gagttgtttt gggggaattt gctgcctgcg ctttcaatcg agcaatttcg ccttttaatt
    15001 ccttgataat cgcttcataa gcatcatagt ctttcataat atcatcaaga aaatcgttca
    15061 cttcatcttg atcatagcca cgaaaaccaa ttttaaaatc ttgctcgaaa atatcttttg
    15121 tcgtaaattt aatacttgcc atctctctcc ctaatctaaa acgtcactac tcctattata
    15181 ttaaaaaaac agggaaaaat caagtcaaat cactcagaat ttgaaaaatt ttcagcctcc
    15241 tcattcaatt cctcaaaggt aagcgttttt ctattatatc cttctttctt taagaccatg
    15301 tgatataaat atttcaaatt ggtttcattt tcagaatcgt aaaataaata acaacctgtc
    15361 gtattatcca gtagaaactg gttatagtct ctaaactgcc ctgggttttc atagcgtgga
    15421 taggcatact ttacaaaatc aacctgctta aaccgtgcta actttgcttg gttagcctca
    15481 ttccactgtt cgccatggtt ttcaaatgga aatattgttg caatactcag ttgatatccc
    15541 tcggctcgta aatcttctaa gacttctaag gaccagtatt caaaacccaa ttgacctgtc
    15601 agtataaacc aactaactcc ctcttctaaa aaacgaataa aattcctacg aattgcagat
    15661 ttaataatgt gcagtcgagg atctttttct gaaaaaattc ccaggtctgt atgtctataa
    15721 cctgttacca aaaggctctt agtatccatt tcttgtactc ttttcgctaa ttatggtata
    15781 atatgctgat attattttat caagaaggag ttctatggtc aattatcctc ataaggtgtc
    15841 caaaaaaatc aatagaacca gtcctatttc atcccagcga gtaaactttg ctaatcgcgg
    15901 aatgtctttt gaggccgcta tcaacgatag caatcaatat taccttgcgc acgatattgc
    15961 tgtcattcac aagaagccaa ctcccgtgca aattgtaaaa gttgattacc ccaaacggag
    16021 ccgtgctaag attgtcgaag catactttcg acaggcatct accacagact actctggtgt
    16081 ttttaaaaga cattatattg attttgaagc gaaagagaca cgccaaaaag catccatgcc
    16141 catgaaaaat tttcatgcgc atcagattga acacatgaaa caggttgtta aacaaggtgg
    16201 tatttgtttt gtccttcttc acttttcaac attaaaagaa acctatctct tgccagccac
    16261 tcatttgatc gaattttatc aagtagacat gggtagtaag tcgatgcctc taacctttat
    16321 tcgacaatac ggctttgaga ttcaaatggg gcgtttcccc agcatcccat atctggaaat
    16381 cgttgaaaaa aatctattag gtggtgaatc ttttgaaaac tacaacaatt aaaaaagctc
    16441 ttctaatcac tgcaaatatt ttacttgcgg gggctatcat tgcctgcctg gccggagctg
    16501 cattggttgt ttactacatc caaagcgctc cagaattgac agaagaatct ttgacagcaa
    16561 ctgtgtcaag taaaatttac gataagaacg gaaatcttat tgcagattta ggagcagaaa
    16621 aacgttcgag tgccaagact gaagaaattc caacagactt ggtaaatgcg attgtcgcaa
    16681 tcgaagacca acgcttcttc aatcatagag gtgttgacgt gattcgtatt gcaggttccc
    16741 tgattaataa cctgagtggt ggacgtttgc aaggtggctc gacacttgac cagcagttta
    16801 tcaagctgac ttatttctcg acatccgtag aagatcaaaa tctaaaacgg aaaattcaag
    16861 aggcttggct agctactcaa ttggaacgtc gcaataccaa acaagagatt ttgacctact
    16921 acatcaacaa ggtgtatatg tcaaatggta attacggtat gaagactgct gctcttgctt
    16981 tttatggaaa agagttaaaa gatttgactc ttcctcaatt agctctttta gcaggaatgc
    17041 cccaagcgcc aaaccaatat gatccttata ccaatccaga agatgcaaaa gcacgtcgcg
    17101 atttggtatt ggctgagatg ctggaagaaa agtacattga taatccccag tatgaacaag
    17161 ctgttctaac acctgtaaca gatggtttgc aaccactatc taatgcagct gcttatcctg
    17221 cttatatgga caactatctg aaacaagtcg ttgaagaagt tgaagcaaaa acaggttaca
    17281 acttgctgac aacagggatg gatgtctata cgaacgtcga tcctgccgcc cagcagcaac
    17341 tttggaatat ctacaataca gatatgtatg tcagctatcc agatgattta ttgcaggtag
    17401 cttcaacggt tgtggatgtt tctaatggta aagtcgttgc gcaattaggt ggtcgtaaac
    17461 aggaaacaaa cgtctcattt ggtaccaacc aatccgttga aaccaaccgc gacttcggtt
    17521 ctacaatgaa gccaatcaca gactatgctc ctgcttttga aaacggcatt tatacatcta
    17581 cagcagattt aatcttagac ggtccttata actatcctgg tacaccaact cctgtcaata
    17641 actgggataa acaatactac ggtaatatct ctgttaagac tgccattcag tattctcgaa
    17701 atgttaccgc cgttaaagca ttggaagcta cgggcttaga aaatgccttg aaatttttga
    17761 atagcgttgg tatcaattat ccagatattc attactctaa tgctatttca agtaatacat
    17821 ccgatactag cagtaagtat ggagcaagta gtgaaaaaat ggctgctgcc tacgctgctt
    17881 ttgccaatgg tggtacttac tacgctccac agtatgtcaa taaaattgtc tttagtgatg
    17941 gaacagtaac agaatatgct cctaaaggga ccaaagtcat gtctgccgaa acagcctaca
    18001 tgatgaccga tatgatgaag gctgttatgt cttacggtta tggattaaac gcttctgtta
    18061 gtggtgttcc tatggccggt aaaacaggta catcaaacta taccgatagc gaaacggata
    18121 ctattctagc ttcaattcca gaagcaaatt atagttacat ggtcgttcct gatgaaaact
    18181 tcgttggata ttctagtcag tacgcaatgg ctgtttggac cggatatacc aatcgtatga
    18241 ctcctatcct tgataacagc atgcgaattg caacggatgt ctaccacaat atgatgctct
    18301 ttatgcattc ggactataca gctaccgatt gggagatgcc aagcggactc gtacgttacg
    18361 gttccaacta ctatctcaga ggtagtcgct cactctccaa tgcttataat acttacacta
    18421 gctctagtac aagttcttct agttcgactg aaactagtga aacaaccgaa gcgacgacaa
    18481 catcaagcga aacatcatct ggaactgcaa ccgagactgc aacttcttca actgaaactg
    18541 caacaacagg aaacaatccc gcaacgggac agacagatgg tcaataagaa aattcccaga
    18601 ctcttctggg tttttctttt gcttaaatta tgataagata agattatgaa acctgaagta
    18661 ttttataaaa ccttggcaga ccaaggtatt caattaactg accaacaaaa acaccaattt
    18721 catcgctatt tccaactatt agtggaatgg aatgaaaaaa tcaatctgac agctattacc
    18781 gaggaaagcg aagtctatct gaagcatttc tacgattcta tcgctccaat cctgcaaggc
    18841 catattcaga acgaacctct tcggctttta gatattggag ctggagctgg attccctagc
    18901 ctacctatga agattatctt tccgcaacta gatgttacca tcattgattc tcttaataaa
    18961 cgaattaact ttttacattt gcttgccgaa gaactagagc tcgaaggtgt tcatttctat
    19021 catggtcgtg cagaagactt tgcacaagat aagaacttcc gtgcacaatt tgaccttgtc
    19081 acagcacgcg cagttgcccg catgcagatt ctttctgaat tgactattcc ttatttgaag
    19141 ctcaacggaa aattaatcgc actcaaggcg tccagcgcag aagatgaatt gacccaagca
    19201 aaaaatgcac tcaatctcct cttcgcaaaa gtcatcgaaa accacgacta tactcttcca
    19261 aatggcgacc ctcgaacatt gaccatcgtc gaaaagaaaa aggaaactcc aaataagttt
    19321 ccacgcaaag ctggtctgcc aaataaaaga cccctttaaa cataagaaaa gaggctgggc
    19381 aaaaagctca acctcgcttc tcagagttcg tgtcaacatc tcagcgcagt ggttgattgg
    19441 cagatttgtt cgtgttttac actccaaatc tgacctaatc aactgtgcgg aggcgggaag
    19501 acgaactctt tttttgacca gtcgagttct ttcccgctcc ctcttttatt atccaatttt
    19561 tgtaatattg gttgcttgaa gaccacgttg gccttgctcc acttcaaatg tcactttttg
    19621 accctcttcc aacgatttga aactattaga ctgaatttca gaaaagtgtg caaatacatc
    19681 tggaccgttt tcttgagcaa taaaaccaaa tcccttttct gcattaaacc atttaactgt
    19741 accttgtacc atttgtgtta cctcttttta tttatttgta aaacttatca agaggaaaaa
    19801 caaaagatga aactgatact taaactctaa atttatttac attccctact gtaacagtat
    19861 atttcctata tgtcaacgat aaagggttga taagcccaat aaactcaata atcatcgcca
    19921 cggattatag aatcgaccct gattaataaa aaatagatag attgtcacga ctactagagc
    19981 aagaataaat agcaagacag cgtaatctat tctttgtaga ggctgttggg tataccatgt
    20041 tcttttttta ttttttccaa aacgccgcaa ttccatagcc gttgaaatcg tatcaatcct
    20101 ctccaaggag ctgaaaatca gaggaatgac aagggataga ttccctttta tacgatccat
    20161 caattttcct ttcttggaca attccaaacc acgcgcttct tgagacattc gaatcgtata
    20221 aaactcttct tgaatatctg gaatataacg catggtcaaa ctgactgcgt aagcaacttt
    20281 gtaggatacc ccaatttgat tcaagctgga agcaaactga ctaggatgag tggtcatgag
    20341 aaagataatc gctaaaggaa ctgtacaaaa atacttcaag agaagattca ccagataaaa
    20401 tgcttgctga ctcgtcaagg aataaactcc cagacctgat agcaagggag tgtctgctcc
    20461 gtaaagttcc actccatacc gtggtgcaaa caagtgaacc atcagggcat tcattgcagc
    20521 aaaaatgatt gtcagaatta aaacaagaga gatttcttta taacgaatcc ctgacatttt
    20581 gaacaaggct agagaaaata ctgcgataaa aagaatcaag cgggtatcat aagtaatcat
    20641 agcaagaatg gaaactatga gaaaaaagag taatttactg acagccgata aactatgaat
    20701 aaagccagtt cctggatggt aaccaattag tttttgttga ctcatcttcc tctcctttct
    20761 tcctgcatat aaaacgtcgt taattccaag ggattaactc ccaacctctc cgccaagtga
    20821 aaaattgatg tttcttttaa attggctcgc tcaatcacca tatcatcaga taaaatttct
    20881 gctggactgg catcttcaat aatctgccca tccaccacaa caacagctct atctgaataa
    20941 tctagcatga gttgcatgtc atgggtaatc atgacaatgg tgtgcccttg agcatttaat
    21001 tggtccaaaa actccatcat ttcccgataa tgccgttgat cttgtcctgc tgttggctca
    21061 tctagcaaaa taatttctgg attgagaacc aagatagatg ctatcgttac ccgttttttc
    21121 tgcccatagg aaagtgccga aatcggccaa ttacgaaacg gatacaggcc acatactttc
    21181 aagatatggt gaactcggtg ttcgatttct tcttctgtca cccctcgcac gcgcaaacca
    21241 agagcaacct catcaaagat catcgtttgg ctaatcattt ggttgggatt ctgaagaaca
    21301 taaccaatcc gttctgctcg ctctttgaca gaatcttctt tgattgactg ccccttccag
    21361 agaatctcac cctcaccctg aataaaacca caaagcacct tggctaaagt agatttccca
    21421 gcaccatttt ttccaacaat agctagacgc tctcccttat gaatcgtcag ttgaatatct
    21481 gtaagaactt gtttgttagg ataggaaaaa tgaagatgct tgacttctaa caaagactct
    21541 ttctctccat tttcaaagtg tagagtcggt agagatgcta tattcaaagc ctcaatatca
    21601 atttcccaaa ctgaggagag gtgttctgcc tgcgcaatcg gatatcctaa ggcgcgaaga
    21661 gtcgatagat ataagggttc acggatgccg ttttcctgta acaatgaggt acgtaataaa
    21721 tcatccagtc ctccattgaa caaaatccga ccatcattaa tcaatacaac tcggtccacc
    21781 tgacgataca gtacgtcctc taaacggtgt tcgataataa tggttgtggt cccttcttgc
    21841 ttgtgcagac ggtcaatcaa atcaatcgta tcttggcctg atttggggtc taaattagca
    21901 agtggctcat caaacaacaa aataggactc tcatcaataa gaactccgcc cagactgaca
    21961 cgttgctttt gacctccaga taaatcttgt ggtcgatgtg ccagcaaatc ggtcaaagac
    22021 agtctttctg cccaatctcc tactttttgc ttcatcacca cctggctttc catgtcgttt
    22081 tccaaagcaa aagctaggtc ctcggcaact gataagccaa tgaattgacc atctgtatct
    22141 tgaagaaccg ttgatacgag caaggactta tcataaactg aaagattaaa agcttcctga
    22201 ccatccaata gtaaacttcc gctatgttcc cccttgtaaa tattgggaat aataccatta
    22261 agacattgtc caatcgtcga ctttcctgaa ccagatgggc caataatcag aactttctct
    22321 ccctgataaa tcgttaactg aatatctttt aaagtgggtt cctgttgcgc ttggtattta
    22381 aatgtaaaat ctttaaattc aatgattttt ttcataggac tattatagcg taaattgact
    22441 ctgaaaaaaa ggaagaatca tcagtttctc ccttttcaaa taacttcatc tatgattcta
    22501 gagtagatga attctaactt cctacatcac aatcacatta aaatgatgcc catcaaggtc
    22561 cgtaaaactt gctccataaa agccacgggc atctgtcggg catcctgtaa tttgcccgct
    22621 atttgcttca acacgctcta taagtgtatc cacttctttc ctactcgcca cattaagcga
    22681 aatcaagact tcattgccac tcgctactac ctgaaatggt acgcgttgag caaagctatc
    22741 tgcctgcatc aaaatcaagt tgccaccctc aggcaaagtc acagacttaa tatctccaaa
    22801 cgtcgcaact gcaaaaccga ttgcttcata aaaccgacca gctattcaag gtctgccact
    22861 gctaatactc aatgaaaatc aaaagtaacc taggaaacga agcgaagata gaactgaagt
    22921 tcatcaaggc aagctgacaa cggataatgt tgattttcga aagggataag ttcatccaaa
    22981 atgacttcat ctccttctct tttccaattg ataggcttcg tatagaatgg actgaaaaaa
    23041 tataaagaat tctgattgca caaaaaaatt gcactgattt ttcaatgcaa ttttggtatt
    23101 actaagcatt tgctggagta agagtttccc caatcgcttc caagcgttct gcccattctt
    23161 gacgagtcaa gccgttttca gagatgtatc ggttgcgctc atgagcacgg cattcagctg
    23221 aacagccacg aacatacttg tgctcattct cttctgatgt caaaatgcga cggttacaga
    23281 atggattgcc acagttgaca tagcgctcac atggtgtgcc atcgaaccag tccttaccaa
    23341 cgacaacagg gttaacatgg ttaacatcga ccgcgatacg ctcgtcaaag acatacatct
    23401 tcccatccca caattcgcca cgaacttctg ggtctttacc ataggttgcg ataccaccgt
    23461 ggagctggcc gacatccttg tagccttcac gaaccatcca gcctgagaat ttttcacagc
    23521 gaacaccacc agtacagtaa acaactacgc gcttgtccat gaatttctct ttgttatcac
    23581 ggacccattg tggcaactca cggaagttgc ggatgtctgg acggatcgca ccacggaagt
    23641 gaccaaggtc atactcgtag tcattacgtg tatccaaaac aactgtatct tcgtccaaga
    23701 gagcttcttt gaactctttt ggtgataggt aggcacctgt tgtcacgagt ggatcaatat
    23761 cgttgtcaaa gtcgttgtct tccaaaccaa ggtgaacaat ttctttttta taacgaacaa
    23821 acattttctt gaaagcttgc tcattttctt catcaatctt aaaccacaaa tcgctgaaca
    23881 atgggtttgc atgaacgtag tccatgtact tttgagttgt ttcatagtca ccagaaacag
    23941 taccgttgat tccctcatca gcaatcaaaa tacgtccttt gagtccgata gacttacaga
    24001 aagctagatg ctcagcagca tattctttgg cattttcaat tggaacatac ttgtaataga
    24061 gtagaacgcg aatatctttt gacatagtgt ctccttttca atctttgcaa tagatgataa
    24121 aactctgtca tttctaataa atgatacctt acgattatat cgtttagccc ctcaaaagac
    24181 aaaaatctga actgaaaatt tagaacctgt attcgcgggt tcaatgctta tatgtaaaaa
    24241 atacggtaac atggatggca tgctaccgca tttattgaag gttcgctata acttcttagt
    24301 cgtctaattc tgttttcagt tgtcggagtt tttccaaggt atcatgtcca atcagttggt
    24361 ccacttttcg cttatggcgt ctagcaatat tttccttcca tttatcttca ataggattat
    24421 gtggcttctc gattttcctt tgatctttga tttctcttgc ctcctgccat tccactcctt
    24481 taataacttc ttcccaatag ctggcaatgg cagagcagag atagagttgg accttattca
    24541 accagtgtcc aaaaaaagtt ttcggagcat tttcctccac tagccgatag gtcctttgga
    24601 tttcaataag ttgactttgg tatttttccc actggagcat agctttactc ggcttttttc
    24661 gataaagttg acgctgtttg attagagcta tatacggtgc aacaaaacgg ctcgtccgta
    24721 tcttgataaa atttctggag gagcgaatgg ccaccagcat gcctgaggcc aataagcgtt
    24781 caacctccct tgcctgatta tctggaaaac ggtaatggac ttgccacccc tcttcttcaa
    24841 ccactaagcg aatctgcatt ctaccatttt gccaactcgc ctccacctct tgcttttcat
    24901 cccataactt ttgaaaaaag agaagaaggt agtagctttg tttactaagt tcataccaac
    24961 tgttcgtgtg ataaaggtag gtccgaatcg ctcctttcat ataaaattcg ccatggaaat
    25021 cataaaaagt tggctgttta tatgtagatt gtgatgggtg gttactaact cggaaaacct
    25081 tataataata actattccaa agaatgcagc cataaattga tttcgtcgtt ttcgattgat
    25141 aaagacttaa aacttcctcg tcaggattga gtatagaatg aataatcggt ctcaactttt
    25201 cttctgtcca gaccatactt ctccccttga acacatgttt ttgtttgttt tatattatta
    25261 tatcatatat gtattttttg gtattgaaaa aaacctagaa ttttttctag ggaaaataaa
    25321 aacttaatct ttagacaaac tacctgtctt cgtttgagat tttgcataaa cagccaaaag
    25381 gagtgtccct gcaatagcaa ctgtaatact gtttgcaata cctgccacaa gtccctgtgc
    25441 aaaaacctta ttcgccgcct cactgtagat aagaatatct aaaactgggg caatgactcc
    25501 ccaagcaagc acattcgccg caatctgaac aaggttaaag aaaagaatat ctttcccttc
    25561 aaaaatccct tcttgaatac gtagacgttt tttggtaaga ccaacaacca aaccaaagac
    25621 ccctgatgcc aaaatccatg accaccatgg accgtaagtc aacgaatcct taagcgtgtg
    25681 cccaatgaaa ccgaccaaga agccaactac aggaccaaat acaactgcca atagcgattg
    25741 aacagcgtat tgaagctgga tagaagtatt tgggacaaag gttggaatat tgattaaatg
    25801 accaataacc acaaagaggg ctgcgccgat accagtagca acgactgttt tgattgaatt
    25861 atttttcata actatctcct aaaatttctt taattactaa tacgtttgat ttcaatatgc
    25921 cagttttgac ctacaccgac attataagcc ttggcaaatg agccctgatt aatcgcaaca
    25981 cctacacgat agagggaatt gatataaatc aatggctgtc cgatacgtac atccgcaaag
    26041 gacttcccgt aggttacttg gttttgatac acaagcatgt cgttattgta tatggtcact
    26101 tcaaagcgat cttcaaactg cggttgcaag gtgtaaaatt cttccctcgt aatcgaggtc
    26161 caaagcgagc cgaatcgaac atccagaata tcaattgcac cttttacata gtccgaacca
    26221 acttccgttg ggactgttgg aatttccaca atcttatcca cgcttaattc tggaccaact
    26281 tcttcaaagc taatgtgtcc agaagccaat ttagctcccg tataggcata aacatctcga
    26341 ccatggaagg tgtacgaatg ttctgtattg gcacggcgat tggccacctc tgaaatttct
    26401 cgaaccgcct ttatccccac atattttttg atgaaggata gcgtcccatt atctggcgtt
    26461 acaatgtagt gattttgttc ggtcaaagct actacactct tacgttttga accaactccc
    26521 ggatcaacaa ccgaaacaaa tgttgttccc tccggccagt attcgaccgt ttgaaagagg
    26581 cgatatgaac cctcaaagat attgtacgga gtaatatcat gagttagatg gtgaacaact
    26641 aaatctctcg attcttggag agcaacacca atcatcgctg aaactgcacc atcaactaaa
    26701 ccaaaatctg attgtaaaac aagtaaatta tttgacatac attctcctaa tcttattacc
    26761 gttctatgat aacagaaaac ctgtgaccat tcaaatagat atttccaaag cctggctata
    26821 gtttttcctt atacctcatc tatcaagcac tctactatca gccaccgaaa actcgcaaat
    26881 ttttccatcg taccttcttc gaacttaagc taaaatatcc ccacttctct agttccaaca
    26941 actatcgtct ttgagtaatt ggatataaag aaaaccgcct agcgcagttc caatttccaa
    27001 acttttaagc caaaaattgt ccttgtggac agctgaaaat aaagaaaacc agctaccgct
    27061 ggttctcatt tccaaccttc gacgtcccac gtacaatgag aatcatctac cgacgatttc
    27121 aatttccagc cttttagtat aaaaaaagtg ctaccagcac ttttttatac taaaacccat
    27181 ccacattcgt gtagattttt tggacgtctt cgtcgtcttc tagggcatcg tagagttttt
    27241 caaatgttgc gaggtcatcg ccttcaagtg atacttctga ttgtggaatc atttcaagtt
    27301 cagtgacgtt gaactcttgg attcctgatt ctttaagtgc cacgattgcc ttgtgcaaat
    27361 cagttggggc tgtatagaca gtgattgtgc cgtcttctgc ttctacgtca tccacttcga
    27421 catctgcttc aaggagcaat tcgaagatgg cgtctgcatc atcacctgca aagacaacca
    27481 cacctttttt atcaaacatg aatgatacag aaccagatgc ccccatgtta ccgccgtttt
    27541 taccaaaagc agagcgcacg ttagctgccg tacggtttac gtttgatgtc aaagtatcaa
    27601 cgataatcat agaaccattt ggtccaaagc cttcgtaacg accttccacg aaggtttcgt
    27661 ctgtgtttcc ttttgccttg tcaatggcct tgtcaatgat gtgttttgga acctgcgctt
    27721 gcttagcacg gtcgataaca aatttcagcg ctgagtttgt ttctggatct gggtcaccct
    27781 ttttcgctgc tacatagatt tcaacaccaa atttggcgta aacttttgag ttagcaccgt
    27841 cttttgcggt tttcttggct acaatattgg cccatttacg tcccatgggg aacctcctac
    27901 attttataaa atcgctttat tataccataa atggcaagga ttgagaagat ttttaaaatc
    27961 ttcttcacta tacagttgac atgtatagtt tctcgatata t
//
LOCUS       DAZQCJ010000019        25935 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000019 DAZQCJ010000000
VERSION     DAZQCJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25935
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.019"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(8..982)
                     /locus_tag="ACHU66_000733"
     CDS             complement(8..982)
                     /locus_tag="ACHU66_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFR3389035.1"
                     /translation="MKQEKRRQLAKIAYLYYEEGKSQAEIAAETGIYRTTVSRMLTKA
                     KSEGIVKIEIQDFDRRLYRLEKYVQEKYGLKGLELVENSTDEDQLDLENRLAQAAADM
                     LTNLIDDGKKVGFSWGRSLSLIVDKIGNHRMKGVKFVPIAGGPSHIHARYHVNTLIYD
                     MANKFRGECSFINATIIQENQELAQGILSSKYFEELRADWKDLDVAAIGIGGRVDEKN
                     RQWLDMLTTADFQALSDEGAVGETCCRGLNQYGQAIVEDLQKRTIAISLDDLKEVPDT
                     IALAYGEEKAQAILAVLRAGYIHHLVTDERTIVKVLELDNDVHVHTNL"
     gene            complement(995..1738)
                     /locus_tag="ACHU66_000734"
     CDS             complement(995..1738)
                     /locus_tag="ACHU66_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR3389036.1"
                     /translation="MERLDKIIQLVSQHEKIDVNSLAEQLDVSKVTIRKDLDKLESKG
                     LLRREHGYAVLNSGDDLNIRMSFRYDVKKRIAKEAANLIADNETIMIESGSTCALLAE
                     EICKTKRNVTIITNSYFIANYVRQYDSCQIILLGGEFQKDSQVTVGPLLHKMIQFFHV
                     DKAFVGTDGYDSEHGFTGKNLMRSEVVQYMSDVAENMIVLTDSSKFTKRGTVRRFGLS
                     QVAQVITDTSLSEDLVKELENARVTVRLV"
     gene            1870..2646
                     /locus_tag="ACHU66_000735"
     CDS             1870..2646
                     /locus_tag="ACHU66_000735"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR3389037.1"
                     /translation="MNTRKGIIFNIQHFSLHDGPGIRTTVFLKGCPLRCPWCANPESQ
                     KRKPEPMLDAVSKKMTIMGEEKSVDDIISEVMKDIDFYEESGGGLTLSGGEIFAQYEF
                     AKAILMEAKRHGLHTAIETTAFVEHHKFVDLIQYVDFIYTDLKHYNTISHRKVTGVNN
                     NLIIQNIQYAFSIGKEIVLRIPVIPDFNNSLEDAEQFACLFNQLKIDKVQLLPFHQFG
                     ENKYKLLNRTYAMTDVKALHPEDLKDYQAIFIKHNINCYF"
     gene            complement(2943..3230)
                     /locus_tag="ACHU66_000736"
     CDS             complement(2943..3230)
                     /locus_tag="ACHU66_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFR3389038.1"
                     /translation="MAKVYEFEAVIHPVPDKGGAYIIFPYDIREEFGKGRVKVHARFD
                     GHPYDGSIVNMGVKDEAGNICYIIGVQKATRLAIGKQAGDRVQVTIQERME"
     gene            complement(3241..3894)
                     /locus_tag="ACHU66_000737"
     CDS             complement(3241..3894)
                     /locus_tag="ACHU66_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFR3389039.1"
                     /translation="MKINELEKRLLAVADASQAQPMKAYMKNHFDFLGVRTPDRRKVA
                     RQFFKESKAQGIDWEFVEACWSKPYREFQYIAIDYLVTKKKDLVLADLPRLKKLAQSK
                     SWWDSIDGLDKLVGKIVLDNPEAKQTILEWSLDDDFWLRRIAIDHQLLQKEKMDTELL
                     EKILVNNLNQIEFFINKAIGWSLRDYSKTNPDWVRAFLKKYSSQMAGLSIREASKYI"
     gene            complement(3884..4393)
                     /locus_tag="ACHU66_000738"
     CDS             complement(3884..4393)
                     /locus_tag="ACHU66_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFR3389040.1"
                     /translation="MIDIRSARIEDAADLVAIYAPYVEKTAITFETEVPTVEAFASRI
                     EKTLEKFPYLVSVEEGKVVGYAYASTYYARAAYDWTVELSVYIKQEARGKGIGSLLYD
                     AIEEDLKARGFKNFLACIALPNPASLSLHKKRGYEQVAHFKNVGYKFDTWHDIVWLQK
                     SLVGKQNED"
     gene            complement(4394..5011)
                     /locus_tag="ACHU66_000739"
     CDS             complement(4394..5011)
                     /locus_tag="ACHU66_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5052 family protein"
                     /protein_id="HFR3389041.1"
                     /translation="MKLQKKIMLSVVLGLSLVSLSACQSIANWWKNTKEEWIGLEMTV
                     RTYDENSQLIDEMSGRSLSISRNQEFDSVDAEGYSNADSSVLKVTLGNYEIDHVGSSL
                     IAAEEGLEDLYAKYQTTVDIANYDRAIPLVNRMVSSLKNDFTGKAKVVLIRSQNGTPL
                     ATYVGDKVSLYASDAPKTSELLIDGKRLIIYRCDYTIYDRELLEN"
     gene            5169..6020
                     /locus_tag="ACHU66_000740"
     CDS             5169..6020
                     /locus_tag="ACHU66_000740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HFR3389042.1"
                     /translation="MKQFCAKHKMKLLIFLVVWSFFSGCKVLLTFFGKPDGMDGKYPL
                     FFLTISLVALYVFPMILIIRYIAKRFDISKKVIHLSWILGITASFYFSGLGQTLLGAF
                     WLFIVKPPQTFIQNWGAAVTAPFHEEFGKGLVVLLVLLLLKKLTLKNAVVSGMVVGLS
                     FQIIEDGLYVFQQIFKSKADGFATLIERMLHAGGTHWAFSLAFAVGLVALVSKNSGMS
                     KKQGLFWMLMAVLAHFFLNTPFNEGLTSNSGEITVLMLCFSFCVALAAFFKVDQIETN
                     QHTSNTY"
     gene            complement(6267..7578)
                     /locus_tag="ACHU66_000741"
                     /pseudo
     CDS             complement(6267..7578)
                     /locus_tag="ACHU66_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631733.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
     gene            7795..8604
                     /locus_tag="ACHU66_000742"
     CDS             7795..8604
                     /locus_tag="ACHU66_000742"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HFR3389043.1"
                     /translation="MKEKFQMCKTYLPVVLATIGFVNGCKIIFGELGKPNGMEAKYPL
                     FLLTISLVAIYILPLLVLTYSLAKRYNISKQVIGLSWLLGLTSSVSFSELGHTAIGYF
                     LLEIVKASNDFLNDWGAAISGPLAEEIGKGLTVLLVLLICRKMTLKNALVSGVIVGLG
                     FQIMEDITFVFRDMFMNKLDGFETILERVGQAGWAHWVFTLLFAIGLVALLTKNTGMS
                     KAQGVFWIGASFGIHFLFNSPFNTGIFQTVFPILSISLGLLAYQTVEKLSE"
     gene            complement(8648..10318)
                     /locus_tag="ACHU66_000743"
     CDS             complement(8648..10318)
                     /locus_tag="ACHU66_000743"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFR3389044.1"
                     /translation="MKTDIEIAQSVTLKPITEIVEKVGISFDDIELYGKYKAKLSFDK
                     INAVKDNAPGKLILVTAINPTPAGEGKSTVTIGLADALSKIGKKTMIALREPSLGPVM
                     GIKGGAAGGGFAQVLPMEDINLHFTGDMHAITTANNALSALIDNHIHQGNAVGIDQRR
                     IIWKRVVDLNDRALRKVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIDDLKER
                     LANIVIGYRFDRSPVYVRDLAVEGALTLILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALRLADYTVTEAGFGADLGAEKFLDIKVPNLPKAPDAVVIVATHRAL
                     KMHGGVAKTELSAENVEAVKAGFSNLKRHVENIQKYGIPAVVAINEFVSDTADEIAAL
                     KELCAEIGVPVELASVWANGADGGVELAETVVATIENQAANYQRLYKAEDSLEEKVTK
                     IVTQIYGGSGVVFEKKARNQLTEFAKNGWDKLPVCMAKTQYSFSDDQFALGAPTDFDI
                     TVREFVPKLGAGFIVALTGDVMTMPGLPKAPAALNMDVAADGTAIGLF"
     gene            10822..11517
                     /locus_tag="ACHU66_000744"
     CDS             10822..11517
                     /locus_tag="ACHU66_000744"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFR3389045.1"
                     /translation="MKLLITSGGTSEAIDQVRAITNHASGNLGKIIAEQALKRGHEVT
                     LVTTRQAVKPDSQKNLTIIEITNVDSLKETLEPLVKSHQALIHSMAVSDYTPVYMTGL
                     DELRATEDSSSLLKKQNTESKISSKDDYQVLFLKKTPKVISYVKKWNPAITLFGFKLL
                     VNVPKEELFAVARQSIERNGADYILANDLKDIGENQHIAYLVDKTREIQAQTKDEIAQ
                     LILNTLEKGEQNG"
     gene            11510..12058
                     /gene="coaC"
                     /locus_tag="ACHU66_000745"
     CDS             11510..12058
                     /gene="coaC"
                     /locus_tag="ACHU66_000745"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFR3389046.1"
                     /translation="MANITLAVTGSISAYKAADLTSQLTKLGHQVTVLMSRSAMDFIT
                     PLTLQSLSKNLVHTDVMIEENPTLIKHIDIAKETDVFLVAPASANTIAKLANGMADNI
                     ITATALALPIGTKKLLAPAMNTNMYLNPATQKNIKTLIEYGFEEIKPREALLACGDFG
                     TGALAEVTDILEKVSNTLDEKK"
     gene            12045..12614
                     /locus_tag="ACHU66_000746"
     CDS             12045..12614
                     /locus_tag="ACHU66_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR3389047.1"
                     /translation="MKKNKSTQIATIAIFFAVMIVINILSSIIFNILPVPIKPTIVHI
                     PVIIASIIYGPRVGACLGALMGLMSMTHNTIILLPTSYLFSPFVENGNVYSIIIAVVP
                     RILVGITPYFVYKWIKNRKGLFIAGAIGSMTNTIFVLGGIFFLFSSVFDGNIQAMLAT
                     VLGTNSIAEMIISSVLTAALVPTLQKIQK"
     gene            12705..14423
                     /locus_tag="ACHU66_000747"
     CDS             12705..14423
                     /locus_tag="ACHU66_000747"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFR3389048.1"
                     /translation="MTYQETYQTWLDFADLPDYLREELVAMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINVFVVRQATEGLAKLVESKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAAHGIKSYVFESLRPTPELSFAVRHYNAIAGIMVTASHNPKEFNGYKVYGEDG
                     GQMPPADADALTNFIRAIDNPFAVELADLEASKENGLITVLGEETDVKYLEELNDLNI
                     NPELIAEYGKDMKIVYTPLHGTGEMLARRALAQAGFESVQVVEAQATADPDFSTVASP
                     NPESQAAFALAEELGREVGADVLLATDPDADRVGVEVRQADGSYWNLSGNQIGAIIAK
                     YILEAHKQAGTLPANAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLMVAEIAAYYRSRGMTLADGIDEIFKE
                     YGYFAEKTISVTLSGKDGAEQIKAIMAKFRDNSPAQFNATDIAVFEDFALQTKTDKDG
                     NIEKLTTPPSDVLKYTLADDSWFAVRPSGTEPKIKFYIATVGETLAEAEEKISNIEKE
                     INEFVG"
     gene            complement(14586..15117)
                     /gene="tnpA"
                     /locus_tag="ACHU66_000748"
                     /pseudo
     CDS             complement(14586..15117)
                     /gene="tnpA"
                     /locus_tag="ACHU66_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775157.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(15275..16021)
                     /locus_tag="ACHU66_000749"
     CDS             complement(15275..16021)
                     /locus_tag="ACHU66_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389049.1"
                     /translation="MPYRKLTSRDRSFIKGQTDRLLNQMNSNAAYLGTVQTYEAAKAR
                     ANQAYLAMELEDDPEKKVRLAKLVENYTAQAQAILDEWEEKNSRTEKTTRNVKLILWL
                     TVLFTLSFVFPVNIILAILFSVWFYRNYLRSSRTDQVIKAANSQSLTEEERFEDWLTE
                     KIVVLNKDKRQWTEGIIAKYDSLDEIAAGIGEMDEIDKVAGEKLLRRYQDILADIEKL
                     PDSEQKTYYLDFHQEIIADLEDLFQQLHFG"
     gene            complement(16346..17224)
                     /locus_tag="ACHU66_000750"
     CDS             complement(16346..17224)
                     /locus_tag="ACHU66_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006532941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFR3389050.1"
                     /translation="MMDDLDDILNDDSLFENFETDRTLFSTKRYQGTVRARSKSSSRT
                     RVQTGFDTYRKLFTQVHADIACGKRQIKSIEETSQGNRISRKNPIKQGNFYVDNGVML
                     YVVKIYDPQTNVEVGQSTERKYKVHIVYENGTENHVWLLSLISSLYDKKRSGRLVTEP
                     LDTISMLGEEAAKYHTTGYIYVVKYAGTDNRFLELDNLYKIGVATDIKKRLSNSQNEA
                     TYLYAPVHLVASYEIQNVSASKLEKFLHDAFSDKRVQLETASPTGKKVSVTEWFVVEL
                     EQIEQKIYELFKQLNL"
     gene            complement(17214..17504)
                     /locus_tag="ACHU66_000751"
     CDS             complement(17214..17504)
                     /locus_tag="ACHU66_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389051.1"
                     /translation="MIENFATLEDIFADEAFESLVAGIRVVKVERLDPEIEKFMEICQ
                     WVKEHGREPQRSTQIKERQLFSRLKAIRADEGRRAQVSAYDELDLLGDRHDG"
     gene            complement(17501..19423)
                     /locus_tag="ACHU66_000752"
     CDS             complement(17501..19423)
                     /locus_tag="ACHU66_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFR3389052.1"
                     /translation="MSQLFEVTYGQKKTSVNTNELGMREMQARVCEKRNSPYLLVKAP
                     PASGKSRALMFVALDKLKRQGLKKAIVAVPEKSIGKSFRSTKLSDFGYYWDWIVKDEN
                     NLTTSGTAKSKVEKFINFIQSTDETDDVLIVTHATLRFASEQLDASDFDACLLAIDEF
                     HHVSQDDNSVLGNALREVLQKSTAHIFAMTGSYFRGDSVPILDHADEARFERVTYTYY
                     EQLEGYQYLKSFVMNYKFYRGRYTDAFPDLLPEFIDKKTIIHIPNVNSGESSKDKYGE
                     VDEILDCLGDYLHKDEHGIIHLRSKTGKILKIADLVTEDGRDLVSNYLAKMDSEDELD
                     MIIALGMAKEGFDWPFAEYALTIGYRSSLTEIVQIIGRVTRDSSNKTKAQFTNLIAEP
                     DAEDEEVQFSVNNIMKAITASLLMENVLSPSFKFKRKENDGQKANGAEIFIKGLAKPS
                     TERTQKIIEQDITDLKASVLQDDVVQAAISAGAESKVITKQLIPRVIATRYPDLTQTE
                     VEEVRQHLVADLTLSNAKKDSENNKLLKMADKFINIDDLSIDLIDSVNPFQGAYEVLS
                     RDVDSRTLSLIARVFDAKKVEFTTDELRMLYPQIKAFQAEHGHSPRRNSSDLEEERLY
                     FALAKLDELRRERDNQ"
     gene            complement(19420..22180)
                     /locus_tag="ACHU66_000753"
                     /pseudo
     CDS             complement(19420..22180)
                     /locus_tag="ACHU66_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006532944.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
     gene            complement(22381..22992)
                     /locus_tag="ACHU66_000754"
     CDS             complement(22381..22992)
                     /locus_tag="ACHU66_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HFR3389053.1"
                     /translation="MELNQWLKSFQLKDNRLVGPFFYGTLLALRFEIGPADEAEELSR
                     AIYLERAYARAVELFEQASSEYDYVLLSLLRQEDRDIDTYLWHFSSKFNFDKVPEPEL
                     IEVEDWTGDVLVFECYLFPVTDQDLKALLREIVKADHGGFHYLSASVIFLSSKDKVLY
                     HCYDDRGVDIAVVDDDKRRQLFTDCHDLLFDYDMEEMVRRMDF"
     gene            23115..23990
                     /locus_tag="ACHU66_000755"
     CDS             23115..23990
                     /locus_tag="ACHU66_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFR3389054.1"
                     /translation="MKTEFINEKAQSAFNLNIITSPIVAIILLLVGDSLGAVIFTPIS
                     FLLPFSETLSVSFELFAFAFISLMVILWARYIEKSPWVGLGFTKKGAGKDFLLGWGIG
                     AAMLTTCTLIMWGLGAIEFTSIQFSTKLVGEFIILILAWSIQGTTEEILTRGWMFSSL
                     SAKHNIPIGIIVSSLFFTFLHLGNNAISLIPILDLTLFAVLACLIMLKTGNIWVIGGL
                     HAAWNCFQGNVFAFPVSGTDAGQAFVQIGITGPEWLSGGKFGVEGSIISLLVQGIMIS
                     WLVYDLYFKPKTSSL"
     gene            complement(24008..24844)
                     /locus_tag="ACHU66_000756"
     CDS             complement(24008..24844)
                     /locus_tag="ACHU66_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFR3389055.1"
                     /translation="MMTSDIVSRAMMTRERTRGTTLLFLPSILLSIVYAVPVTIFRLL
                     TFLELRQNSAVVEGRLVSSIRLTYLGELGFALLLQLFIGLSCFHLLELLRGDRQEFSL
                     KDSFSFLKTEWKKPICTTFLYYYLLLFMAGLPANLGAALFIKGFAMSSLMVLSPGAMA
                     ELAVHQPSQLMKIGAPIFLLGMIIYLFVYYTYSQVVFVLYDHLDNASYTGPLAVFRES
                     RFLMKGNYWQRIWLDLSFLGWFIGEVFTLHLLSFFVTPYYHLSRTIFYEKLKKQRLSA
                     VE"
     gene            complement(25037..>25935)
                     /locus_tag="ACHU66_000757"
     CDS             complement(25037..>25935)
                     /locus_tag="ACHU66_000757"
                     /inference="COORDINATES: protein motif:HMM:NF017927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFR3389056.1"
                     /translation="ERRNAMSNSDIRAKATVIREQTDGMITLFLAPVLISFLSGVAEF
                     GLKQIWGQTGTFSWGTTIVKDGVTWTHNFISVGPSIIFDFIAQCLIVTACFQLIRLVR
                     KQRTKVNFTECFTLLDGKNFLPVFSTTLLKMLVLYLASFPAALGMILIGLAFYSTIVI
                     FGAPIDYQPDFSAFFSSSYFQTGLILVCVGITCSLFVGYGLSQVSFLLYDYLENNTYS
                     SPFKLFSQSWQLMKGNKWRRFLLDLSFIGWFIGVLLTFGFLGLYVYPYYWTCQTIFYE
                     DLKAKNPLMFSTYKSVISSTVA"
BASE COUNT         7653 a         5796 c         4676 g         7810 t
ORIGIN      
        1 tactctctta caaatttgta tggacatgca catcattatc cagttctaaa acttttacga
       61 tggttcgttc atctgttacc agatgatgaa tgtagccagc tcgcaataca gctaaaatgg
      121 cttgagcctt ttcctcccca tatgcaagag ctatggtatc tggaacttct tttaaatcat
      181 ctaaagagat ggcaatggtc ctcttttgca aatcctcaac gatagcttga ccgtattgat
      241 tcagaccacg acaacaggtt tcaccgactg caccttcatc tgataaggct tgaaagtcag
      301 ccgtcgtcaa catatctaac cattgacgat ttttttcatc gactcgaccg ccaataccta
      361 ttgctgctac atctaagtcc ttccagtcag cacgtagctc ctcgaaatat ttagaagaaa
      421 gaataccttg agcaagttct tgattctcct ggataatcgt cgcattgata aaactacatt
      481 ctccacgaaa tttattggcc atatcgtaaa tcagagtatt cacatgataa cgtgcgtgga
      541 tatgacttgg accgccagct attggaacaa atttgacacc tttcatacga tgattcccta
      601 tcttatctac aataagactg aggcttctcc cccatgaaaa accaactttc tttccatcat
      661 caatgagatt ggtaagcata tcggctgcag cctgtgccag acgattttct aaatcaagct
      721 ggtcctcatc ggtgctgttt tcaaccagtt ctaaaccttt taatccatat ttttcttgaa
      781 catatttttc taatcgatat aggcgcctat caaaatcttg aatttctatt tttacaatgc
      841 cttctgactt agccttggtc aacattctac tgactgtcgt tctataaatt cctgtctcag
      901 cagcaatctc agcttggctt ttcccctctt cgtagtaaag ataggctatt tttgccaact
      961 gccttctttt ttcttgcttc atactgcctc caacctaaac tagccttact gtgactctag
     1021 cattttctaa ttccttgaca agatcttcag aaagcgacgt atctgtaatg acctgagcaa
     1081 cttgacttaa accaaatctg cgtactgtcc ctcgttttgt aaatttacta gaatctgtta
     1141 acacaatcat gttttcagca acgtcagaca tgtactgtac tacttcgctt cgcattaagt
     1201 ttttacctgt aaaaccatgc tcactatcat agccgtcagt tcctacaaaa gccttatcaa
     1261 catggaaaaa ctgaatcatt ttatgcaaga gcggcccaac tgttacttgg gaatcttttt
     1321 gaaactcacc acccaataaa ataatctgac agctatcata ttgtcgcaca taattggcta
     1381 taaaataaga atttgttatg atggtcacat ttctcttggt tttgcaaatt tcttctgcca
     1441 agagagcaca agttgaccca gactctatca taatcgtttc attgtcggcg ataagatttg
     1501 ctgcttcttt tgcaattcgt ttttttacgt catagcgaaa ggacatccga atattaaggt
     1561 catcgccact atttagaaca gcatagccgt gttctctacg taataaacct tttgattcta
     1621 acttatctaa atctttacgg atggttacct tagaaacgtc cagttgctcc gccaaactat
     1681 tgacatctat tttttcatgt tgtgatacca gttgtataat tttatctaat cgttccattt
     1741 catcacctca tcattattct atcaaatttt gactcttttt caactatttg caacctttca
     1801 acttacgttc gtaattgtaa aactttcttt tgtgtgataa aatcaaatta atcgaaaagg
     1861 agaaagagca tgaatacacg aaaaggcatt atttttaaca ttcaacattt ttcgctccat
     1921 gacggtcctg gtatacgtac gacggttttc ttgaaaggtt gccccctacg ttgtccttgg
     1981 tgtgcaaatc cagaatctca aaaacgaaaa cctgaaccca tgcttgatgc tgttagtaaa
     2041 aaaatgacca tcatgggaga agaaaaatct gttgatgaca tcatatccga agtcatgaaa
     2101 gacattgatt tttatgaaga atctggtggt gggctcacgc tgtctggcgg agaaattttt
     2161 gcccaatatg agtttgcgaa agctattctt atggaggcaa aaagacacgg ccttcatacc
     2221 gctattgaaa ccaccgcttt cgtagaacac cacaaatttg ttgaccttat ccaatacgtt
     2281 gactttatct atactgattt aaaacattac aatacaattt ctcatagaaa agtcaccggc
     2341 gtcaacaata accttatcat acaaaatatc caatacgcct tttccatcgg gaaagaaatc
     2401 gtcctacgta tccctgttat ccctgacttt aataattccc tcgaagatgc tgaacaattt
     2461 gcctgtctct ttaatcaact caagattgac aaagttcaac tactcccctt ccatcaattt
     2521 ggagaaaata agtataagct tctcaatcga acctatgcta tgaccgatgt caaggctctc
     2581 catccagaag atttaaaaga ctatcaagca atcttcatca aacataatat caactgttat
     2641 ttctaacttt gagaaataca aaaacttcct cggctgagcc aatagcattc ctatagaaat
     2701 cacatactga aatatgcttc tgatagagta tctgaagcat acttagtata taaaaaaggc
     2761 ccaccataaa ggtgggccag ggcatcgttt ccgatgagaa tagaaggttc acacaactat
     2821 tcaaggtccg ctcctgcgtt tcaattaaat gaagacctcc ctagcgtcga aagtgacaaa
     2881 gtcactactc ggctcatcgc atgaatctat tctaccataa tctcaaggat ttgcaagcct
     2941 gattactcca ttcgttcttg aatggttact tgtactctat caccagcctg ttttccaata
     3001 gccagtcgag tagccttttg aacgccaata atataacaga tattgcctgc ttcatcctta
     3061 actcccatat tgacaatgga gccatcatag ggatgaccat caaatctggc atgtaccttt
     3121 accctcccct tgccaaactc ttctcgtata tcatagggaa agatgatata ggctccgccc
     3181 ttatccggta ctggatgaat gaccgcttca aattcatata cctttgccat aacagccctc
     3241 ctagatatat ttactagctt cacgaatgga taagcctgcc atttgggaac tgtacttttt
     3301 tagaaaagcc cgtacccagt caggattggt cttggaataa tctcgcaaac tccagccaat
     3361 cgccttgtta atgaaaaatt caatctgatt gagattgttg accaaaatct tctcaagaag
     3421 ttccgtatcc atcttttctt tttggagaag ctggtggtca atggcaatcc gcctcaacca
     3481 gaaatcatca tccaaactcc attccaagat tgtctgcttg gcctctggat tgtccaaaac
     3541 aatcttaccg actaatttat ccaagccgtc gatactatcc caccaagact tactttgagc
     3601 cagttttttc aaacggggta aatcagctag aaccaagtct tttttcttgg taaccagata
     3661 atcaatggcg atatattgaa actcacggta gggcttagac cagcaagcct ctacaaattc
     3721 ccagtcaatt ccctgagcct tagattcttt gaaaaattgc ctagcaactt ttcggcggtc
     3781 aggagttcgc acacctagaa agtcaaagtg attcttcata taggctttca tgggttgagc
     3841 ttgactggca tccgccacag ccaacaaacg tttttctaat tcgttaatct tcattttgct
     3901 tacctacaag agatttttga agccagacaa tatcatgcca ggtatcaaat ttataaccaa
     3961 catttttgaa atgtgctacc tgttcatacc ctctcttctt atgaagagaa agcgaggctg
     4021 gattaggaag agcaatacag gctaaaaagt tcttaaagcc acgcgctttc aaatcttcct
     4081 ctatggcatc atataaaaga cttccgattc cttttccacg tgcttcttgc ttgatatata
     4141 cggataattc caccgtccaa tcgtaagccg cacgggcata gtaggttgaa gcgtaggcat
     4201 aacccacaac tttaccttct tctactgaga ccagataagg aaacttctcc aaggtctttt
     4261 caattcgact tgcaaaggct tcgacagtcg gtacttctgt ttcaaaggta atagcggtct
     4321 tttctacata aggagcataa attgccacaa ggtctgccgc atcctctatc cttgcggaac
     4381 gaatgtctat catttagttc tccaataatt ctctatcata aattgtataa tcacaacggt
     4441 agataatcaa ccgcttgcca tcaattaaga gttcagaagt cttaggtgca tcagatgcat
     4501 aaagagacac tttatcacct acatatgtag caagcggggt tccgttttgc gaacgaatca
     4561 aaaccacctt tgccttacct gtaaaatcat ttttgagact agataccata cgattaacaa
     4621 gcggaattgc acgatcgtaa tttgcaatat ctactgtggt ctgatatttc gcatacaagt
     4681 cttccaaacc ttcttctgct gcaatcaaag atgagcctac gtggtcaatt tcgtaattcc
     4741 caagtgtcac cttcaagaca gacgaatccg cattggaata gccttcggca tccaccgagt
     4801 caaattcctg attccgcgaa atcgaaaggg atctaccaga catttcatct atcaattgag
     4861 aattttcatc gtatgtcctt accgtcatct ccaaaccaat ccattcttcc ttggtatttt
     4921 tccaccaatt cgcaatggac tgacaagcag acaaactaac aaggctcaaa ccaagaacaa
     4981 cagataacat gattttcttt tgcaatttca tataatagtc ctccaacata ttttacttat
     5041 tatatcacgg aatacaaaaa ccgagaaaaa atagatagac ctgagataat aaaaagatgg
     5101 caatgataaa ctatagcacg gtagggataa ctgacatcta ctaaattaat ataaaggaag
     5161 tcatttttat gaaacaattc tgtgctaaac acaaaatgaa gctacttatt tttctagtcg
     5221 tttggagttt tttctctggc tgtaaagttc ttcttacctt ttttgggaaa ccagatggaa
     5281 tggacggaaa gtatcctctc ttctttctaa ctatttcctt agttgcactt tatgtcttcc
     5341 ctatgattct tatcattcgt tatatagcga aacgttttga tatttctaaa aaagttatcc
     5401 atctcagttg gattttgggt ataacagcta gtttctactt ctctggtctt ggtcaaacgc
     5461 ttttaggagc attttggtta ttcatcgtca aaccacctca aacatttatc caaaactggg
     5521 gagcagctgt caccgctccc ttccatgaag agtttggtaa gggcctagtt gtactactcg
     5581 tactcctatt gctaaaaaaa cttactctaa aaaatgccgt agtcagcggt atggttgtag
     5641 gtttgagttt ccaaattatt gaagacggtc tctatgtctt ccaacaaatc tttaaatcta
     5701 aagcagatgg cttcgccact cttatcgaac gtatgcttca tgctggtgga actcactggg
     5761 ccttctccct tgcattcgca gtcggtttgg tcgctctcgt ctcaaaaaat agtggcatgt
     5821 cgaaaaaaca aggacttttc tggatgctga tggctgtctt agcacacttt ttcttgaata
     5881 ctccattcaa cgaaggacta acatctaact ctggtgaaat aaccgttctc atgctctgct
     5941 ttagtttctg cgtggcactt gctgcatttt tcaaagtaga ccaaattgaa acgaatcagc
     6001 acaccagtaa cacatactga aacaagcctg cattgacagg cttttgttat gttacaaacc
     6061 aagtattcga aatggttgtt gattcatcaa tatctattat cagtctttat cccattccat
     6121 cggtccaaga aatcgcagat aattataaaa aaccgcccat tacggacggt catagtcagt
     6181 caatgaaata aggtcgctcg atgatcttga cactagtcta ggaaacgcag ccacaactaa
     6241 tacaagaagc ttatctccct agagtattaa aaattaaaga cgtcattcaa attctcagcc
     6301 atagtcgggt gagtgaaaat ctgtgttttg aagtagctat aaggaatctt attgtcaatc
     6361 gccatagcaa tcaagttaat cagctcctct gaattgcgac caaagagagt cgcccccaga
     6421 acaagcttac tttccttatc cacgataacc ttgaaaatgc ctttgaggtc attattgaca
     6481 tgggcacgtg gcatattggc gacaggcaat tcgttggcaa tgtagtcgta acctgcttcc
     6541 ttggcttcct tctcggtcag accgacacga gacagcacag gcgtaataaa gacgctggtt
     6601 ggaatagact tacgttggct caagctataa gttccagtcc cagtcaactt gccgaaaacg
     6661 atacggaagt cgtctaggga tgtgtaagtg aattgtggtc cgccattgac atctcccacc
     6721 gcatagacac ctggaacagt cgtttcgcag tagtcatcca ccttaacagc accattttcc
     6781 aaaagctcaa tcgccgtatt ttccaagccc aagtcagcag tgttcggcac ccgacctgtc
     6841 gcatagagga cggcgtcgaa gattgccgtt tcgccattaa ccgtcacagc aacttgctcg
     6901 cccgcagctg ctacttgctc gagtttcgca cccaagacaa aggtcacgcc agcttcttcc
     6961 atatactcct tggctaactt agcaaccact tcctcttctc tcggtaaaat agcagagctg
     7021 acttcatata ccgtcacctg gctgccgagt ttgctgtaaa gcccagcaaa ttccaatcca
     7081 atgttgcctc caccgataat tgccaacgtg ttaggacgag tttccaagtt ctgaatgcct
     7141 gtgctgtcat aaacgtgagc tgtatccaac aagcctggaa ttggaagcac gcgcgactta
     7201 gcacctgtat tgatgacgat ggtttccgca gttagttgaa tggattcctc accagccgat
     7261 acttccacaa ccttgtctgc aacaaaacgt gcgtgtcctt gatacaaact agcaccactg
     7321 cctttcaaga ctgcctcgtt tttgtttcgc agacgagtgg tgactgtttc tttttgatcc
     7381 attacctgct caaaagttca gtttttatct gctgctacca agagggtctt ggttggaata
     7441 cagccaatat tgatacaagt cccgccaaac atagcagtat tttcctcaac caaggcaacc
     7501 tttttcccag ctactggcag cttacctgct aaggtcttta catccaaaac caatgaccaa
     7561 taaatcatat tgttccataa catactcctt ttatttttta aaccttagtc tagcaaaaaa
     7621 gaaaatttga gacacaattc cgttacggct ctattatgga gtcctagttg caggaaaaaa
     7681 cattacaata accgataagg tttagataaa aaataggcga attatagaca aaatgtactc
     7741 atttttgata caatgtatcc atacaccagt ttattaatac agaaaggtca aactatgaaa
     7801 gaaaaatttc aaatgtgtaa aacctatctt ccagtcgtct tagcaacgat tggatttgtc
     7861 aacggttgca aaattatctt tggagaatta ggtaagccaa atggcatgga agctaaatat
     7921 cctctctttc tcctaaccat ttccttggtc gctatttaca tcctccccct actagtactg
     7981 acatattcct tagctaaacg ctataacatc tccaaacaag ttataggtct tagctggctt
     8041 ttaggcttaa caagtagcgt ctctttttct gaactgggac atacagctat cggatatttc
     8101 ctgctcgaaa ttgttaaagc tagtaatgat ttcctaaatg actggggtgc agctatttca
     8161 ggtccattgg cagaagaaat cggtaaaggg ctaactgttc tacttgtact gcttatttgt
     8221 agaaaaatga cactaaaaaa tgcattggta agcggagtga ttgttggatt aggtttccaa
     8281 atcatggaag acatcacctt tgtttttaga gacatgttca tgaataaatt agacggcttt
     8341 gaaaccattc ttgaacgcgt tggtcaagct ggttgggccc attgggtttt cacgcttctc
     8401 tttgccattg gcttagtagc ccttctcacg aaaaatacag gtatgtcaaa agcacaggga
     8461 gtattttgga tcggtgcaag ctttggaatc cattttctct ttaattcacc attcaacact
     8521 ggtatcttcc agacggtgtt tcctatcttg agtatttccc ttggcttact tgcctatcaa
     8581 acggttgaga aactatctga atagacaaaa aatccgcctt tgagcggatt ttttatattc
     8641 attcttgtta gaacaagccg atagctgtcc cgtctgctgc cacatccatg ttgagagctg
     8701 cgggagcttt tggcaaacca ggcatggtca tgacatctcc agtcaaggca acgataaagc
     8761 ctgcacccaa ttttggcaca aattcacgga ctgtaatgtc aaagtctgtc ggcgctccaa
     8821 gggcaaactg atcatctgag aagctgtact gggtcttggc catgcagact ggcaacttgt
     8881 cccaaccatt cttagcaaat tcagtcagct ggttacgagc tttcttctcg aaaaccacac
     8941 ctgagccacc ataaatctgc gtaacaatct tggtcacttt ctcttccaag ctatcctcag
     9001 ccttgtaaag acgttggtag tttgctgctt gattttcaat agtagcaaca actgtctcag
     9061 ccaattcaac accaccatca gcgccatttg cccaaacact tgccaactcg acaggtacgc
     9121 caatttcagc gcaaagttcc ttcaacgcgg caatttcgtc agctgtgtca gatacaaatt
     9181 cattgatagc aacgactgct ggaataccgt atttctgaat attttcaacg tgacgcttca
     9241 agttagaaaa accagctttc actgcctcta cattttcagc agaaagttct gtcttggcta
     9301 caccaccgtg cattttaagc gcacggtggg tagcaacaat gactactgca tccggagcct
     9361 taggcaagtt cggcaccttg atatctagga atttctctgc tccaaggtct gcaccaaaac
     9421 ctgcttctgt tacggtatag tcggccaaac gcagagctgt tgtagttgca agaactgagt
     9481 tacagccatg agcaatatta gcaaatggac ctccgtggac aaaggctggc gtgccataga
     9541 tggtttggac aaggtttggt ttaatagcgt ccttcaaaat aagagtcaaa gcaccttcca
     9601 ccgccaaatc acgcacgtag acaggactgc gatcgaaacg ataaccgata acaatatttg
     9661 ccaaacgttc tttcaagtca tcgatgtctg tcgccaagca taaaatcgcc atgatttcag
     9721 aagctacggt aatatcgaat ccatcttcac gcggaatacc attgagcgga ccacccaatc
     9781 caacagttac tttacgcaaa gcacggtcat taaggtccac gacccgtttc cagatgatcc
     9841 tgcgttggtc aatgcctaca gcatttcctt gatggatatg gttgtcaatc aaggctgaaa
     9901 gagcgttgtt agctgtggta atggcatgca tatcgcctgt aaaatgcagg ttgatatctt
     9961 ccatgggcaa gacttgtgca aaaccaccac cagcagctcc gcccttgatt cccataacag
    10021 gccccaaaga aggctcacgg agggcaatca tagttttctt tccaatttta gacaaagcat
    10081 ccgccagacc aatggttact gttgacttac cttcaccagc aggcgttggg ttgatagctg
    10141 tcaccaagat caatttccct ggcgcattgt ctttcaccgc attgatttta tcaaaggaca
    10201 attttgcctt gtattttcca tagagttcaa tatcgtcaaa gctgatacca actttttcaa
    10261 cgatttctgt gattggtttc aaggttacac tttgtgcaat ttcaatatct gttttcatga
    10321 gagctcctaa tttccttatt ttttatggac attataccat accacagctt attttcgcat
    10381 attttttact atttatgcac taacgtacgg aattttcaga aaaactacag gtttctttac
    10441 ttaaacagat acaaattttt aaaacctgca ttctcatcat agctctacac ttccatctaa
    10501 gactgggttt agttagtttt ttcaaaagag agtcagtatt cttttaaaaa ttgccttgat
    10561 gagcaagcaa acgaccgctt gcataaagca cttcacttat atagtctttt agtttacttt
    10621 tagtacgagg taatgagccg caggcataac tgaagttagg caaggcgagt taacgaagta
    10681 ataaaagaaa aactaaatga cgataataca ggcccttata tttttttgta ttagcagcat
    10741 cattttccag tgtcacagat tgattagcaa atcgattagt gcaaacttta cgaaaaatag
    10801 aaattctagt aaaatatgaa catgaaacta ttgattacat caggcggaac aagtgaagct
    10861 atcgatcaag tccgcgctat tacaaaccat gcttctggaa atcttggaaa aatcattgcc
    10921 gaacaagcct tgaaacgcgg tcatgaagtt actcttgtga ctacaagaca ggctgttaaa
    10981 ccagattctc agaagaattt aaccattatc gaaatcacaa atgttgatag tttgaaagaa
    11041 acattagaac ctttagtcaa atcacatcag gccctgattc atagcatggc tgtatccgat
    11101 tatacacctg tttatatgac tggtttagat gaactaagag caactgaaga cagttctagt
    11161 ctactaaaaa aacagaatac agaaagtaag atttcatcca aggatgacta ccaagttctt
    11221 ttcttgaaaa aaactcccaa ggtcatttcc tatgtaaaaa aatggaatcc agctatcaca
    11281 ctctttggct tcaaactatt ggttaatgtt cctaaggagg aattatttgc cgtagctcgc
    11341 caaagcattg aacgaaatgg cgctgattat atacttgcta atgacttgaa ggatattgga
    11401 gaaaatcaac acattgccta tttggttgat aaaacaaggg agattcaagc acagacaaaa
    11461 gatgaaattg ctcagctcat tttaaatacc ctagaaaaag gagaacaaaa tggctaatat
    11521 cacacttgcc gttactggct ctatttcagc ctacaaagca gctgacctaa ctagtcaatt
    11581 gaccaagctc ggtcatcagg ttacagtcct catgagtcgc tctgctatgg acttcatcac
    11641 acctttgact ctccaatcct tgtcaaaaaa tctggttcac acagatgtca tgattgaaga
    11701 aaatcctact cttatcaagc atatcgatat tgccaaagaa acagacgtat ttttagttgc
    11761 ccctgcttct gccaatacga tagctaaatt agctaatggt atggctgaca acattattac
    11821 ggcaacagct ctggctctgc caattggtac taaaaaactg ctggcacctg ctatgaatac
    11881 taacatgtac ctcaatcctg ctacccagaa aaatatcaaa accctcatag agtatggttt
    11941 tgaggaaatc aaaccgcgtg aagccctgct tgcttgtgga gattttggaa ctggggcctt
    12001 ggctgaggta actgatattt tagagaaagt gagtaacacc cttgatgaaa aaaaataaat
    12061 caactcaaat tgctacaatt gcaatcttct ttgctgtcat gattgtcatc aatatattga
    12121 gttcaattat ttttaatata ttgcctgttc caataaaacc caccatcgta catatcccag
    12181 tcattattgc ttctattata tacgggccac gtgttggggc ttgtttgggg gctctcatgg
    12241 gcttaatgag tatgacccat aatactatta tcttactacc aaccagctat cttttctccc
    12301 catttgttga aaatggtaat gtttattcaa tcatcattgc agttgtccca cgtatattag
    12361 tcgggattac accttatttc gtctacaaat ggataaaaaa tcgtaaagga ttatttattg
    12421 ctggtgcaat tggttcaatg accaatacta tttttgtact tggaggtatt ttcttcctct
    12481 tctcctctgt ctttgatggt aatattcaag caatgttagc aaccgtttta ggtaccaatt
    12541 caattgcaga aatgatcatt tcttctgtat taactgcagc gcttgttcca actttacaga
    12601 aaattcaaaa ataaatatca gaaaatcatg tgaaagcgtg atttttcttt tcaaacatgg
    12661 tataatgaaa gcgtttaata gaaaaatttt taaggagata tgcgatgact tatcaagaaa
    12721 cctatcaaac atggctcgac tttgcggacc ttccagatta cttgcgtgaa gaattagtcg
    12781 caatggatga aaaaacaaaa gaagatgcct tctatacaaa tcttgaattt ggtacagctg
    12841 gtatgcgtgg ctatattggc gctggtacca accgcatcaa cgtattcgta gtccgtcaag
    12901 ctactgaagg tttggcaaaa ttggtagaat caaaaggtga agaagctaaa aaacgtggtg
    12961 ttgccatcgc ttacgattca cgtcacttct cgccagaatt tgcttttgaa tcagctcaag
    13021 ttttggcagc gcatggtatc aaatcttatg tatttgaaag ccttcgtcca actcctgagt
    13081 tgtcattcgc tgtgcgccat tacaatgcca tcgcagggat tatggtgact gcaagccaca
    13141 acccgaaaga attcaacggc tacaaggttt acggtgaaga cggcggacaa atgccacctg
    13201 ctgatgcaga tgcattgacc aactttatcc gtgccatcga caatccattt gcagttgaat
    13261 tggctgacct tgaagccagc aaggaaaatg gtttgattac ggttcttggc gaagaaactg
    13321 acgttaaata tcttgaagaa ctcaatgacc tcaacatcaa ccctgaattg attgctgaat
    13381 acggtaaaga catgaagatt gtctatacac cgcttcatgg tacgggtgaa atgttggcgc
    13441 gtcgtgccct tgcacaggct ggttttgaat ctgtccaagt tgttgaagca caagcaactg
    13501 ctgatcctga cttctctact gtcgcttcac caaacccaga gagccaagca gcctttgccc
    13561 ttgcagaaga actcggccgt gaagtcgggg cagatgtcct tcttgcaact gaccctgacg
    13621 ctgaccgtgt tggtgttgaa gttcgtcaag ctgacggttc ttactggaac ctgtctggta
    13681 accaaatcgg cgctatcatt gctaagtaca tccttgaagc ccacaagcaa gcaggtacac
    13741 ttcctgcaaa cgctgccctt gctaagtcaa tcgtatctac tgagttggta actaagattg
    13801 ctgaaagcta tggcgctacc atgttcaacg tcttgactgg tttcaaattc atcgctgaga
    13861 aaatccaaga gttcgaagaa aaacacaacc atacttacat gtttggtttc gaagaaagct
    13921 ttggctattt gattaaacca tttgtacgtg acaaggatgc tatccaggct gtgcttatgg
    13981 ttgctgaaat tgctgcctac taccgttcac gtggcatgac cttagctgac ggtatcgatg
    14041 aaatcttcaa agaatacggc tactttgctg agaaaacaat ctctgttacc ctttctggta
    14101 aggatggtgc agaacaaatc aaggcaatca tggctaagtt ccgtgataac tcaccagcac
    14161 aattcaacgc tactgacatc gcggtctttg aagactttgc ccttcaaact aagacagaca
    14221 aagatggaaa cattgaaaaa ctcactactc ctccatcaga tgtcttgaaa tacaccttgg
    14281 cagatgattc ttggttcgct gttcgtcctt cgggaacaga accaaaaatc aaattctaca
    14341 tcgcaaccgt tggtgaaaca cttgctgaag cagaagaaaa aatctccaac atcgaaaaag
    14401 aaatcaacga atttgttggg taagagtcac ataaattaga aagtacaaac cacccgtgaa
    14461 aacgggtagt ttgtacactg gctataagcc cacatcacca gccagcgcct aaagacgctg
    14521 gctttcactt tgttcaagcc tcactgcttt tgactcgtca ctagcctctt aaagaggcat
    14581 tcgtactact tgccattatc cctaaaggga tcttcatact cttttacact caatttatca
    14641 agtgctatat catgtttaac tcgctttcta gatttaagct cgtgaagaaa tcatccactg
    14701 gataatttct ttgttcttgt atatatttct taattgtggc ttcattaagt ccaacttaca
    14761 ctcttcaaca attccccaaa ttgttgaagc cctctgtcca gaaatgacga ttgccaaatt
    14821 tatatttgag attagcgtgt ttatcaaaca tcataagagc actcttacct tttaaatagc
    14881 ccataaagct tgatacactt aatcgcggag gaatactgac taacatatga acatgatccg
    14941 gcatcagatg tccttcgata atttctacac ctttataact acacaaacgt cgaaatatct
    15001 ctccaagact acttcgatat tgattataga tgatttttcg tctatactta ggtgtgaaga
    15061 caatgtggta gaacacaacc actttatatg tgataaacta tgtgcctttt gtgccatact
    15121 tttctcctct cgctttacaa ttggcttgaa cacctttatt gtatcgcgtt tggagttttt
    15181 ttggtataac cttcgtcgcg caccttacgg agcgcgacgg actaaaagtc acataacaaa
    15241 accggcaaca gccttgtact gttaccggtt tttcttatcc aaaatggagt tgttggaata
    15301 gatcttccaa gtcggcaatg atttcctgat gaaaatccaa ataataggtt ttctgttcag
    15361 aatctggcag tttttcaata tctgctaaaa tgtcctgata gcgtcgaaga agcttctcac
    15421 cagctacttt atctatctca tccatttcac cgatacctgc tgcaatttca tctaaactgt
    15481 catattttgc gattatcccc tctgtccact ggcgcttgtc cttgttgagg acgacaatct
    15541 tttctgtcaa ccagtcttca aacctttcct cttctgtcaa agattggcta ttagcagcct
    15601 taatcacttg gtcagttcta cttgaacgaa ggtaattccg ataaaaccaa acggaaaaaa
    15661 gaatggctag aatgatattg acggggaaga caaagctaag ggtgaacagt acggtcagcc
    15721 acaagatcaa cttgacattt ctggtcgttt tctctgtacg actgtttttc tcctcccact
    15781 catccaatat agcctgggct tgagcagtat agttttcaac caattttgcc agtcgcacct
    15841 ttttctctgg gtcgtcttcc agttccattg ccagataggc ttggttggcc ctagccttgg
    15901 ctgcttcata ggtctgaact gtccccaaat aggccgcatt gctattcatc tgattcagca
    15961 aacggtccgt ctgccctttg ataaaagacc gatctcttga tgttaattta cggtaaggca
    16021 tagcaacccc ttcctgtata tcctagatat attactattt tacgataggt tgtgtgaatt
    16081 tgcaatgatt aaagacatta tttccgtcta ccaaataaga gactgaaaat aatgaatcca
    16141 cctgccaagg caacaataat cactggtgaa acccagagta aaatcaaaaa ccaaataaca
    16201 agtttgtcca tctgtccgtc cttctacttc ctgcgaaaga atgtgaaagc tatggctatg
    16261 gcgataatgc ctagcagata acaaatcgca aatgtgaaaa tagaaacgaa caatatattt
    16321 ctccactggt ttagctgata agatgtcaga gatttagttg cttaaaaagc tcataaattt
    16381 tttgttcaat ctgttctaat tctaccacaa accactcagt aacagaaact tttttgccag
    16441 ttggtgaggc agtctccagt tgaacccgtt tatcactaaa tgcatcatgt agaaatttct
    16501 ctaacttact tgcggaaaca ttctggatct cataactggc taccagatgg actggagcat
    16561 acaggtaagt cgcttcgttt tggctatttg aaagccgttt tttgatgtct gtagctaccc
    16621 ctatcttgta gaggttatcc aattctaaaa atcgattatc tgttccagcg tatttgacaa
    16681 cataaatgta gcctgttgtg tgatattttg cggcttcctc acctagcata gaaattgtgt
    16741 ccaagggttc tgttaccaat ctacccgaac gttttttatc atatagtgat gaaatcaaac
    16801 tcaagagcca aacatgattc tccgttccat tttcatatac gatatgcacc ttgtacttac
    16861 gctctgtaga ctggccaact tccacattgg tctgagggtc ataaattttg acaacataca
    16921 gcataacacc attatcaaca tagaaatttc cctgtttaat tggatttttc cgactgatac
    16981 ggtttccttg actggtttcc tcgatggatt ttatctgacg tttaccacaa gcaatatcag
    17041 catgaacctg tgtaaacaat ttccgatagg tgtcaaagcc agtttgaaca cgtgtacgac
    17101 tactagattt actcctagct ctaactgttc cctgatagcg tttagtgcta aataaagttc
    17161 tatctgtttc gaaattttca aacaaggaat catcatttaa aatatcatct aagtcatcca
    17221 tcatgtctat ctcctaacaa atccaattca tcataggcgc taacttgagc tcttctcccc
    17281 tcatctgcac gaatagcctt cagtcgactg aataattgtc gttctttgat ttgagtactt
    17341 ctttgcggct ctcgtccatg ttccttaacc cattgacaga tttccataaa tttctctatt
    17401 tctgggtcta atctttcaac tttgacaaca cgaatcccag caaccaagct ttcaaaggct
    17461 tcatccgcaa aaatatcttc taaggttgca aaattttcta tcattgatta tccctttcac
    17521 gacggagttc atcaagctta gctaaggcaa agtaaagtcg ttcttcctct aaatctgagg
    17581 aatttcgtct tggtgaatgt ccatgctcag cctgaaaagc cttaatctgg ggataaagca
    17641 tgcgcaattc gtcggttgta aactctactt ttttggcatc aaatacccgt gcaatcaggc
    17701 ttaaggtacg actatctaca tccctggaca gaacctcata agcaccttga aatggattga
    17761 cactatcaat caaatctata cttaagtcgt caatattgat aaatttatcc gccattttta
    17821 acagcttatt attttcgctg tctttcttag cattggataa ggtcaaatca gcgactaaat
    17881 gctgacgaac ctcttcaacc tctgtctgtg tcaaatctgg ataacgagtc gcaatcactc
    17941 ttggaatcaa ctgtttggtg atgaccttgc tctctgcacc tgcagaaata gctgcctgta
    18001 ccacatcatc ttgcaagaca gaggctttta agtcagttat atcttgctca atgatttttt
    18061 gagtacgttc cgttgatggc tttgctagac ccttgataaa aatctcagct ccgttagctt
    18121 tctggccgtc attttcctta cgcttaaact tgaatgaagg agataaaaca ttttccatca
    18181 acaagctagc agtaatagct ttcatgatgt tatttacaga gaattggacc tcctcatctt
    18241 cggcatctgg ttctgcaatt agattggtga attgggcttt ggtcttgttt gagctgtccc
    18301 tcgtgacccg tccgataatc tggacaatct ctgtcagcga agagcggtag ccgatagtca
    18361 gggcatattc tgcaaatggc cagtcgaaac cttccttagc catcccaaga gcaataatca
    18421 tatccagctc gtcttcacta tccattttcg ccagataatt gctgactaaa tcacgaccgt
    18481 cctcagtcac caagtccgca atcttcaaga tcttacctgt cttagaacga agatgaataa
    18541 tcccatgctc atctttatgc aggtaatctc ctagacaatc taaaatctca tccacctctc
    18601 catacttatc ctttgaagac tcacctgaat taacattggg aatatgaata atggttttct
    18661 tgtcgataaa ctcaggcaat aagtctggaa aggcatccgt gtagcgacca cggtaaaact
    18721 tgtagttcat gacgaaggac ttgaggtatt gatagccttc caactgttca tagtaagtat
    18781 aggtcacacg ctcaaaacgt gcctcatctg catgatccaa gataggtaca ctatctccac
    18841 ggaaatagga accggtcatg gcaaagatat gggccgttga tttttgcaag acttcccgca
    18901 gggcattgcc caatacagaa ttatcatcct gggagacatg atgaaactcg tcaatggcaa
    18961 gcaaacaagc atcaaaatca ctggcatcca gttgctccga agcaaagcgg agcgtggcat
    19021 gggttacgat caagacatca tctgtctcat cagtagattg gataaagttg ataaattttt
    19081 ctaccttgct tttggcggta cctgatgtgg ttaagttgtt ctcatctttg actatccaat
    19141 cccagtaata gccaaagtcc gaaagtttgg tcgaacgaaa agacttgccg atggactttt
    19201 ctggtacggc tacgatagct tttttcaagc cctggcgttt caacttatcc aaggctacaa
    19261 acatcagtgc cctagactta ccggatgctg gcggggcctt gaccaagagg taggggctgt
    19321 ttctcttttc acagacacgc gcctgcatct cacgcatgcc caattcattg gtattgacgc
    19381 tggttttctt ctgaccatag gtcacttcaa aaagctggct catgattgct ccttcatttt
    19441 cttatagcgt ttcaagaggt ggctgaggcg ttcttcatcg ctggtaaatg gtgtttcctt
    19501 ataggcgcgt tcaacaatgc tatctagtac ctgatgggct tctcggagtg cttgtggcat
    19561 ggtatcacga tgataaagct ctgctaaggt accacctaat tcttcccgta agtcaaggat
    19621 ttcaaaaact tgttcttcaa tctgattttt tcgcttagtg gataactctg gtacagggaa
    19681 ggtgttgtaa accagaccag cagagtatcg ataacgagtt tctaacttcc caccgatagc
    19741 ccgcaaccag gtcatgtgaa gccgtgacat gagaagacca agcagataga caggggcgtt
    19801 gtagacagcc atggcagaat cagaaatgat ggtatcttct ccaacaaatc ccataggaac
    19861 atagtcacga ctttcagatg atactcgagg aaccaagagc tgaacaaact catctgcacc
    19921 tgattgtttg aaggtcgagt aagcttcgtc ccactcacct ttttgaacaa atgcataagg
    19981 tgtttttgct gcatcttgag tggactttgc agggctactc atcctagcct gcgctacacc
    20041 cttaatcctt aaacctaaca atgagttatt ctgaacagtt tgatattctt ctttattcag
    20101 ccaaaacaca tatcgtggtt tatctttgat atactcttca ctgccgatat atctcttaat
    20161 aaacggctca agatcagggt ttccttctac tatcttattc ctttcattta agtcagaaat
    20221 aatcagatga ccgccatctc taggcatact accgaacacc attttaggca atctattaat
    20281 cggactatta ctactctcaa ccagtatatc ttccccgcta gctaagtagg gattgatatg
    20341 agtaaccgtt tgacgtacca aggtttcatc aaaaatatat ttttggtaac ttgcattgtt
    20401 cttatttgcc aatccaacta tagcaacagt taccccagca ttatttgcag cgctgttccc
    20461 ccacttgaat gatgtataag caaactctat ctccatttta tataatacag gttgccaaat
    20521 aaaagcaact tgttcccctt gaaaaataga gttggttgtt acaaaagcaa gtttgctatt
    20581 cgaagtattt atgaaatcaa tccctttgta gaaccagcct gcaatgtagt ccattttctt
    20641 atacttgaat tgttcaccaa tcgtcaggcg taaatcttct ttctgttctt tattttgcaa
    20701 tttttgcccc aatataaggc ggatttccta tcaggtatat ttcatcggtt ggggtatgag
    20761 ggacaacttt atcccagtca agtctcaggg catttccttg agtgatgttt ccagcatcac
    20821 gcagagggag aaaggcagat tgtaaattga tttcatttag tgcttctaaa ttcatttgat
    20881 attcagcaat ccacagagat aatctagcta cttcatgtgc aaaatcatct aattcaattc
    20941 cataaaactg attcaagcta atcttcgacg tttcgaagaa atcaattttt ccatcttgtc
    21001 gtagttctcg tagtgctttt aatagtctta tttccaaacg acgaagctct ttataggtga
    21061 tgataaggaa attccctgag ccacaggctg ggtcaaagaa ttttatcttg gataggtgtt
    21121 cgtgaagtgt ggacaattct ttttcgtaag ccgccctatt ctctcgcctg gttcgctcgg
    21181 ttatgtcctt gtcttcgttc tcattgattt tatctacaat agtctggaaa tttgcagtca
    21241 gttcatcaag aaagagcggt ttaatcactt tcatgatgtt ttcaaccgaa gtatagtgca
    21301 taccagagct tgaacggtgc tcactgctag ccactgtctg aatcatggcc cctaaaatat
    21361 ccggattgat ttcattccag ttgaggagct cgcctgcttc caagattaat tttcttgttt
    21421 tcttatcaaa aactagatcg gttgatgcat ctgtaaaaag ttttccattg acatatggaa
    21481 attcctgtaa ccagttttct tggctaagac gattagcaat atccagactg gcaaatatct
    21541 gattaaaaag agtattcaga tcactgccgt cctctcgcga gcgagttttg atggcattgg
    21601 taaaaatacc ttttttcatg atttctgtat cttcggcaaa taaaagaaaa agtaagcgaa
    21661 tcaaaaacag gttaaactct ttttcctctt gactacggtc atcaaagtga ttgattttct
    21721 ttaattcatc atacaaacgt gtgaaccgtt ccgcagcctt aatatccgca ggattttcct
    21781 tggcataatc taccttctct acaccattcc aagccaagaa gaaatcggca taggtcggca
    21841 aatccttaaa agggatctgt aaggtatcga aagtcttggt atccttggct gcaaagtcca
    21901 caaagttggt cacaatgata taacggggtt gtgatttttt atccttgatt gcttcttcaa
    21961 tctcagcaac acctgccaca accgacttat cagtaggaac cgtcttgtaa tacaatttgt
    22021 tcttgatttc accactttgt tctttacgga ttcgggtgat agctgttttt gaaatatcat
    22081 aaaaagacaa gaattcatag ataaaagaag cttggtcaaa accatcctcc aataactgct
    22141 gaatcttgtc ttcgattgta ataatatcaa actttgccat aagatctcct agcttttcct
    22201 aacacatttg tattagtaca aaaggagcgt ttgatgaaaa aataacccac gttattatac
    22261 taaacacacc attcagtttc ctaatcttat tttgtgatag gactgttgtt gttagggttt
    22321 gactgaaagt caaacccatg gtatacgtag tatatcatgg gcatttttat attagttttt
    22381 ttaaaaatcc atcctcctca ccatctcctc catatcatag tcaaaaagca ggtcatgaca
    22441 gtccgtaaaa agctgacgtc gtttgtcatc atctaccacc gcgatatcca ctcccctatc
    22501 gtcatagcaa tgatacagaa ccttgtcctt gctagataga aagataactg aagcagaaag
    22561 gtagtgaaaa cctccatggt cggctttgac aatctccctc aacaaagcct tcaggtcctg
    22621 gtcggtaact ggaaagagat agcactcaaa gactagcaca tctcccgtcc aatcctccac
    22681 ttctatcaac tcaggctcag ggaccttgtc gaagttaaat ttcgatgaaa aatgccagag
    22741 gtaggtgtct atatccctat cttcctgtcg aaggagcgaa aggagcacat agtcgtattc
    22801 tgaactagcc tgctcaaaaa gttctactgc acgtgcatag gctctttcaa gatagattgc
    22861 tcttgataat tcttctgctt cgtctgcagg cccgatttca aatctaagag caagaagtgt
    22921 gccataaaag aatgggccca ctagtcgatt atctttcagt tgaaaggact tcagccattg
    22981 attcaattcc atatactatt ctccttatat agctttgtac tattctacca tatactagga
    23041 aattaggtct agtcttcttt ggaaaaatat gatagaatag tgaactagta aagtatctag
    23101 taaaaggagg tcctatgaaa accgaattta tcaatgagaa ggctcaatca gctttcaatc
    23161 tcaacatcat cacctctcct attgtagcta ttattttgtt gctagtagga gattcactag
    23221 gagcagttat tttcactcca ataagctttt tactcccatt tagcgagacc ctttctgtaa
    23281 gttttgaact attcgctttc gcttttatca gcctgatggt cattttatgg gcacggtata
    23341 ttgaaaaaag cccgtgggta ggacttggtt ttactaaaaa aggtgcggga aaagattttc
    23401 tactcgggtg gggaatcgga gctgcaatgc tgaccacttg tacactcatc atgtggggat
    23461 taggcgcgat tgaatttaca agcatccagt tctctacaaa attagtcgga gaatttatta
    23521 tccttatcct tgcctggtct atccaaggta cgacagaaga aattctaaca agaggatgga
    23581 tgttctcatc actttcagct aaacacaata ttcctatcgg tatcattgtt tcttccctct
    23641 tcttcacatt tctccacctc ggaaacaatg ctatttcact cattccaatt ttggatttaa
    23701 ccctatttgc cgttcttgcc tgcctcatta tgctcaaaac aggcaacatt tgggtgattg
    23761 gtggattaca cgctgcgtgg aactgtttcc aagggaatgt ttttgctttc ccagtcagcg
    23821 gcaccgatgc tggtcaggcc tttgttcaaa tcggtatcac tggtcctgag tggctatctg
    23881 gtggaaagtt tggcgtagag ggctccatca tcagcttgct tgtccaagga ataatgattt
    23941 cctggttggt ttacgatctc tatttcaagc ccaaaacaag ctccctataa ccataaaaga
    24001 ctgttagcta ctctacagct gacagtcttt gttttttcaa tttttcgtag aaaatggtcc
    24061 tggataaatg atagtaagga gtcacaaaaa aagataacag atgaagggta aatacttccc
    24121 cgataaacca tcctaaaaag gataaatcta accaaatacg ttgccaatag ttccctttca
    24181 tcaagaaacg actttcccta aatacagcca ggggaccagt ataactagca ttatccaaat
    24241 ggtcatacaa gacaaaaaca acctgtgagt aggtatagta gacaaaaagg taaataatca
    24301 ttcctagcag gaaaatcgga gcccctatct tcattaattg actaggctga tgaactgcca
    24361 gctctgccat tgctcccgga gataagacca tcaaactact cattgcgaag cctttaataa
    24421 ataaagcagc ccctagatta gctggaagtc cagccataaa aagaagcaaa taataataaa
    24481 gaaaagtggt acagataggc ttcttccatt ctgtctttag aaaggaaaaa ctatctttta
    24541 aagaaaactc ctgtctatcc cctctcaaca attccaataa atgaaaacag gacagaccga
    24601 tgaacagctg taacagcaga gcgaagccta actcacctaa ataagtcaac ctgattgaag
    24661 aaaccaatcg cccttccaca acagcactgt tttgacggag ctctagaaat gttagtaggc
    24721 gaaaaattgt tactggtaca gcataaacaa tagatagcaa aatggagggc aaaaatagta
    24781 gtgttgtacc tcttgtccgc tcgcgagtca tcatcgctct tgatactata tcactcgtca
    24841 tcatcacaat tcccctccaa ttttctctcc ttatttatga agtcacgtcg caaatattcc
    24901 atcaacatcg gactacttta ccatcctact attagtataa aactttatcc attaattttc
    24961 cagaacatct cttaaaaaca ggaaaatccc tccaaaatac gaagggattc ctaataattt
    25021 gttatctcca tactccctag gctactgtgg aagagataac tgatttatat gttgaaaaca
    25081 tcaatggatt cttggccttc aaatcttcat aaaatatagt ctgacaagtc caatagtatg
    25141 gatatacgta aagtcccaaa aacccaaacg tcaaaagaac tccaataaac cagccgataa
    25201 aagataaatc caagaggaaa cggcgccatt tattcccctt catcaactgc cagctctgac
    25261 tgaagagttt aaatggtgaa gaataggtat tgttttccaa ataatcatag agaagaaaag
    25321 agacctgact gagcccatat cctacgaata aactacaagt aatccctaca cataccagta
    25381 ttaatcctgt ttgaaaataa gacgaactga aaaaagctga aaaatcaggc tgataatcaa
    25441 taggagcacc aaagatgaca atagtactat agaaagctaa acctatcaat atcattccaa
    25501 gagcagcagg gaaacttgct aaatacaaga ccagcatttt caataatgtc gtactaaaaa
    25561 ctggtaaaaa attcttccca tctagtaaag tgaaacactc cgtaaagtta accttggttc
    25621 tttgctttct caccagtctt attagttgaa aacaagctgt cacaattaaa cattgtgcaa
    25681 taaaatcaaa tatgattgac gggcccacag aaataaaatt atgcgtccaa gtaacaccat
    25741 ctttcacaat agttgtcccc caggagaaag taccagtttg cccccagatt tgtttcaagc
    25801 caaattctgc cactcctgac agaaaagaaa tcaaaacagg tgccaaaaac aaggttatca
    25861 tcccatctgt ttgttcacga ataaccgttg ctttagctcg aatatcgcta ttagacattg
    25921 cgtttctcct ttcat
//
LOCUS       DAZQCJ010000020        24466 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000020 DAZQCJ010000000
VERSION     DAZQCJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24466
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.020"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(82..1251)
                     /locus_tag="ACHU66_000758"
     CDS             complement(82..1251)
                     /locus_tag="ACHU66_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFR3389057.1"
                     /translation="MFRLAKEELEKLGAMITATEIYQQPGLWKEAHQLYFDQLEKIES
                     FLKSIKEKHDFVHVVFTGAGTSDFVGQSIANYLNQVNDLKHIRFSAIGTVELVSRPHD
                     YLQADLPTILVSFARSGNSPESVAAVEVAKQLVDKLYQVTITCAPEGKLALAAEGDVE
                     NLLLLQPAGSNDKGFAMTGSYSCMALTALLVFSPASQEEKAAWVEIIARLGQDVLDRE
                     DYVQDLINLDFERVIYLGSGGFYGLAHEAQLKILELTAGKIATMYESPLGFRHGPKSF
                     INEKTLIFLFASNDAYTRQYDVDLLNEVHGDGIAERIVALSADKLVGTEAANFVLAEG
                     GADLPDVFLTFPYILFAQTVSIMAAIKCNNLPDTPSPTGTVNRVVQGVIIHPLEK"
     gene            1530..1829
                     /locus_tag="ACHU66_000759"
     CDS             1530..1829
                     /locus_tag="ACHU66_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR3389058.1"
                     /translation="MMNMQNMMRQAQKLQKQMEKSQAELAAAQFTGSSVQDLVTATFT
                     GDKKLVSIDFKADVVDADDIETLQEMTVQAVNAALTKVDEATQKKLGAFAGKLPF"
     gene            complement(1869..3629)
                     /locus_tag="ACHU66_000760"
     CDS             complement(1869..3629)
                     /locus_tag="ACHU66_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR3389059.1"
                     /translation="MKKIQREFQKIYYNLDKILLLFFTLFTFMEFVWIPLNSWISEGL
                     LAMTGHAYLSPTNLLSVFAENLLVTGLFILLFFVNIAIAYLELALLFTGVWQLLDEKV
                     KHLPDYLRDVRDSMVAIIRHSSLPKVLFLLFYSVILLPFLRRVLNIYYFNKIVVPQFI
                     VDYLSNTVWLGILILIFLLLFFWLAARFMYALPNIYFEHRGVKESIAYSWEKTRGRKQ
                     IGSLFRLIWLITFPVLLFTGVGGLFYLVQLLAEHFVPSASYWLAVVCYALLKLAYYGV
                     VALFMMGFVSLLTGKQLPICRRKRLRHHLRLAILLVSGLVFGTQGALALYFPFDTLPV
                     TISHRGVDDGNAVQNSIEALEKTSQLKPDYIEMDVQETKDGQFIVMHDTDLLALTGNP
                     GGTHDYTLAELTGMTASENGMVAPVPSFDEYLEKADELGQKLLVEIKVTKADSPKLTQ
                     NFLKKYGQRLIAKGHQMQSLDYRTIIEIKQYSEKLVSFFILPFNSIYPTTVADGYTME
                     YTSLDQNFMTKSWIRGKSVYAWTPNEEESMTKMLLLQVDGIITDNMTDLQSLMEEMKE
                     NRRYPDLFFQQFQSLIYNFE"
     gene            complement(3820..4746)
                     /locus_tag="ACHU66_000761"
     CDS             complement(3820..4746)
                     /locus_tag="ACHU66_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HFR3389060.1"
                     /translation="MRQTKEMRLVYSDFLAQVGQEDTTIAAIEADLSSSMATNKLSSV
                     LGGRYVNVGIMEQEMVGVAAGLSLLGYKPYIHTFGPFASRRVYDQVFISLAYAQLNAT
                     VIGSDAGVSAEMNGGTHMPFEELGLMRLIPKARVYEVSDDVQFQAVLKETIKVDGLKY
                     IRTIRKATTAIYQGGEDFSKGYCVLRQGEDMTLLASGIMVEQALKAADQLEAQGVSVQ
                     VIDLFRIKPIHEDIPALLSGRPVLTVENHNRIGGLGSSICELMATDLTTPVHRIGIEE
                     SFGQVGQQAYLMDVYGLTAEYIVKQAQLMLQQ"
     gene            complement(4743..5591)
                     /locus_tag="ACHU66_000762"
     CDS             complement(4743..5591)
                     /locus_tag="ACHU66_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR3389061.1"
                     /translation="MTSGTADLKRFAKEIRYHTLATLNQLGFGHYGGSLSIVEVLAAL
                     YGEILDIRVQNFSSKERDHFILSKGHAGPALYSTLYLKGFFDRDFLLSLNQNGTHLPS
                     HPDRNLTPGVDMTTGSLGQGMSVATGVAYGKKIEQSPYYTYTLVGDGELNEGQCWEAA
                     QFAAHHELSKMILFVDDNKKQLDGRTHDICQTFDFVEKFQAFGWESVRVNGADIDAII
                     NAILTMKSSKSPQPKCIVLDTTKGQGVTFLEELESNHHLRLSGQLREDLEQVTLVLAR
                     ELEVME"
     gene            complement(5593..6939)
                     /locus_tag="ACHU66_000763"
     CDS             complement(5593..6939)
                     /locus_tag="ACHU66_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFR3389062.1"
                     /translation="MVLDFLIDIAKTPAILVALIAILGLALQKKPAADLIKGGLKTFV
                     GFIVVGGGANIIVGSLEPFGQMFEQAFNLKGVVPNNEAIVAMALDKYGTATSLIMLLG
                     MVFNIAIARFTRFKYIFLTGHHTLYMACMIAVIMSVAGFTSVGLIVMGGLALGLIMTL
                     SPAFVQKYMIQLTGNDHVALGHFSALGYWLSGVVGGLVGDKSKSTEDINFPKGLAFLR
                     DSTVSIAISMSLIYMIVALFAGGDYIKENLSGGTNSMIYALTLGGTFAAGVFVILSGV
                     RLILAEIVPAFKGISEKLVPNSKPALDCPVVYPYAPNAVLIGFISSFAGGIVSMIILA
                     LTGGVVILPGVVPHFFCGATAGVMGNASGGVRGAVCGAFMQGVLISFLPVFLLPVLGD
                     LGFAGSTFSDADFGLSGIFLGVLAKNGGILTVSVGIFAVLALMLGLSLVKKEQKEG"
     gene            complement(6993..7277)
                     /locus_tag="ACHU66_000764"
     CDS             complement(6993..7277)
                     /locus_tag="ACHU66_000764"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR3389063.1"
                     /translation="MLRIGTACGSGLGSSFMVQMNIESILRDLGVSDVHVEHYDLGGA
                     NPSEADVWIVGRDLADSATHLGDVRILNSIIDMNELRELVTAICQEKGLV"
     gene            complement(7279..9321)
                     /locus_tag="ACHU66_000765"
     CDS             complement(7279..9321)
                     /locus_tag="ACHU66_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFR3389064.1"
                     /translation="MVDNRTMAILMELFATRKESLYELSIQTGIEKEQLHSNLELVNQ
                     LLQEHSFPQIQYKDSEFVISTELYNQKESVFSLFRNRQIYLSQEERQLLIYLYTFIRK
                     EFVSNVHYQELLSVSRNTTLTDIKNVKELCLDFQVRLEYTRAKGYHLIGREEDKHRLA
                     LYALSNCLQSSIGVWALDYILKSWGEENPIEELKIVSQQACNYYKVSALEERLDEYLY
                     FLIFLFIRQARVGNRINWNFEGKGGFVKDFLQRLWKLLRLKSTSTLELNAATQEYLSH
                     LLQGCLEGESGEKDDLFYHLTVEIVEEMERLSLIAFEHRSEMIEGLQRHLIPAYYRLT
                     SRLVNVNSYTETIKEEHADLFYLVKKALRPLEEHLGFTIPDSEVSYFVIHFGGYIEAG
                     QQRSYRYRALVVCPNGVSSSLIVKENLRQLFPNIYFADTHSLQDWKMLDMTDYDMVFG
                     TIKLNIELPFFLVSQLMTSNHKKELFHLVNQHFPNAAYFPIEIEQLLSLVGKHATIHQ
                     EQALKYELVQFLNQRSHEQRGRSPMLEELITKETFQWSEEVLDWQKAVALVARPLVDK
                     GAIETRYIDAMIAKVEEFGPFIDLGKGIAIPHARPEDGVNEVGMSMLVLDKPVYLLDD
                     PSHEIRLFICIAAVDNQTHLRALSHLTKILRAEENIQQLVGAKEFADVQHLLKEEQ"
     gene            complement(9642..10136)
                     /locus_tag="ACHU66_000766"
     CDS             complement(9642..10136)
                     /locus_tag="ACHU66_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019783378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFR3389065.1"
                     /translation="MGIEKTVSELAEILGVSRQAMNNRVKSLPEEFVDKNDKGVTVVN
                     RAGLIKLEEIYKTTIFEDEPISDEVKHRELMEILVDEKNAEIIRLYSQLKAKDKQLAE
                     KDEQLKVKDVQIAEKDKQLDQQQQLTLKAMADKEVLKLELDEVKAQAEEVQAKGFFAR
                     LFGK"
     gene            complement(10306..11706)
                     /locus_tag="ACHU66_000767"
     CDS             complement(10306..11706)
                     /locus_tag="ACHU66_000767"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFR3389066.1"
                     /translation="MVTEQREYRFPEGFLWGSSTSGPQSEGSVAGDGKGWNNWDYWYS
                     QEPELFHQQIGLEQTSTFYEHFQSDLDLLVETGHSIFRTSIQWSRLIPEGIGEVNPQA
                     VEFYRAVFAGIRERGIKLLVNLYHFDLPYVLQEKGGWENKEIVWAYEAYARQCFSLFG
                     DLVDHWITFNEPIVPVECGYLGSYHYPCKVDAKAAVVVAYHTQLASSLAVRACHQLHP
                     YHKISIVLNLTPAYPRSQSPEDLQAARIAELFQTRSFLDPSVLGQYPRELVDILEKHG
                     LRPETTEEELTIIKENRVDFLGVNYYQPLRVQAPSEDGWNGEATFLSQYFQPYDKPDK
                     KINPHRGWEIYEEGLYDIAKNIQENYGNIEWLVTENGMGVEGEDVFEENGMIQDDYQI
                     HFMEDHLIQLHRGIDEGANCKGYMVWTFIDCWSWLNTYKNRYGLVSLDLQTQKRTVKK
                     SGYWFKQVSAKNGFIR"
     gene            11944..13119
                     /locus_tag="ACHU66_000768"
     CDS             11944..13119
                     /locus_tag="ACHU66_000768"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR3389067.1"
                     /translation="MNTVDTIIIGAGPAGMMAAISSSFYGKKTLLLEKNKRLGKKLSG
                     TGGGRCNVTNNGTLEDLLAGIPGNGRFLYSVFSQFDNHDIMNFFQENGVKLKVEDHGR
                     VFPTTDRSQTIIKCLEMKMLENGVNIRTGTEVVSVRKIDDLFHVKTSEETFTAPQLIV
                     TTGGKTYPSTGSTGFGHDIARHFKLEVTEIEAAESPVLTDFPHKKLQGISLDDVTLTY
                     GKHVITHDLLFTHFGLSGPAALRLSSFVKGGETAFLDALPTHSDQDLFEHLEANREKS
                     VKNALRELMPDRLADFFAENYDCKVKQVSQKDLTDLVSLLKALPIKITGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVPGLHFAGEVLDINAHTGGFNITYCLATGWVAGSSYSS"
     gene            13360..14052
                     /locus_tag="ACHU66_000769"
     CDS             13360..14052
                     /locus_tag="ACHU66_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFR3389068.1"
                     /translation="MPINSFGQKIGMPLPHYSSGERPSLETIQGHTVRLEKLSLHHAD
                     DLYQFYGPSAKKTDWTYLPLEPFEDRATFQDYFQTLLNSTDPYYFAILDQATNQAVGT
                     FALMRIDTNHRTIEVGWVIFSPQLQRTRQATEAHYLLMTYVFEELGYRRYEWKCDSLN
                     QASRVAANRLGFTYEGNFRQALVYKERNRDTAWFSLLDHEWPDRKRALENWLKDSNFD
                     GQGQQKQPLSSF"
     gene            14133..14831
                     /locus_tag="ACHU66_000770"
     CDS             14133..14831
                     /locus_tag="ACHU66_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR3389069.1"
                     /translation="MQLKLSSVSKKFDHKIILEDTSFTFEQGKIYGLLGRNGAGKTTL
                     FNCIARNLTLDGGSIQFVKDEIAFDYDNTDIGFTQTYPQLPAFMTAYEFVRFYMDIHK
                     DKLKSPRSPEEWLNLVGIGEEDRHRLLKDFSHGMQNKVQLLLSLIVQPPVLLLDEPLT
                     SFDPVAAHEFKQLIREAKKDSVIIFSTHILQLAQDLCDEIVLLHHQELQAVPSDKLHD
                     PDFEQEVVTLLTAD"
     gene            14841..16466
                     /locus_tag="ACHU66_000771"
     CDS             14841..16466
                     /locus_tag="ACHU66_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389070.1"
                     /translation="MKTILRYFWYQEKYNLYRTVNGFFYYLRKLPLVGKSIPESIFKS
                     YSFKSGLFLILHVLSIPSRFLVKGIWLALNFYLASFWINLLSGGDLTFWNILPGTWLL
                     GFSLWMVFVGYTYRFGKGFEPFIVKSEREFMQNFGLSQSSFLQSQLFVEPIITSLFYL
                     PALLIFSGLSGNWLYLPLGLLTIPTGSFTGQALNRWFFNRRILSRRNSWQSWVILGTG
                     LAAIACLILFRNHLSPIFLLPVLVCQVLLIWFGYGYLKQQTNHLDYLYYCMDQSLQMD
                     KKIFEMTKGNEYTRQGLQMQAKLSMETGKDLTHLSGMTYLNALLFDRYRSVLTKKLHF
                     RLGCLLAIVLFIEGIRWFSKDDIQISNTNYIEGLPFAFMVMYAASMGKAVAQMVFVNC
                     DISMLHYPFYREAKTIIAGFHYRFLQTVKLNAAFSGSLLLALIIFTRAQLPIETYLLT
                     ALLLLSLTALFSFHDLFIYYILQPFTKDMEVVNPVYKFLSGALYWVAYLNIKLDLGSH
                     MYILLISIAMITYVSIGYWILLKKAPQTFRLKS"
     gene            complement(16766..17788)
                     /locus_tag="ACHU66_000772"
     CDS             complement(16766..17788)
                     /locus_tag="ACHU66_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR3389071.1"
                     /translation="MVKENAKGVGLCFLLALVGQWLGHLFPLVGGPVFALLIGMSLYP
                     YFSSKNAFQSGLTFISKKILQYAVICLGFGLNLSAVLAVGRQSLPIIFSTISFALILA
                     FLMWKILPISSHLATLIGVGTSICGGSAIAATAPIIQADDEDVAQAISVIFLFNVLAA
                     LVFPTLATWLGFSTDSGQVFGMFAGTAVNDTSSVTAAASTWDSLYGLGSQTLDTAVTV
                     KLTRTLAIIPITTVLAIWQTRGKGVQADKKSLLAGFPVFILYFILASLITTIAGGFGI
                     GADWFAPLKSLSKVFICMAMVAIGLRTNVFALVKNGRAALLVGLVCWFGVTALTLVWQ
                     AILGIW"
     gene            complement(17798..18121)
                     /locus_tag="ACHU66_000773"
     CDS             complement(17798..18121)
                     /locus_tag="ACHU66_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFR3389072.1"
                     /translation="MIKTNILLIILMAALVTWVPRVLPFVLTQNKSLPPKLVKFLSFL
                     PITIIFALTLSSIMDEKVGSLPSFLPVESLAILPTFFVVLKTKNILLAVLVGVVTTAL
                     LRLIF"
     gene            complement(18108..18809)
                     /locus_tag="ACHU66_000774"
     CDS             complement(18108..18809)
                     /locus_tag="ACHU66_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFR3389073.1"
                     /translation="MKKYAFREGMRDAIPTVLGYASIGLACGVVSVNSGISTVEMGLM
                     SLLIYAGSAQFVMCAMIFAGAPLLSIAVTVFFVNLRNFLMCLHATTIFQGNRLGSNIL
                     IGSFVTDESYAVLLRKQIEHHTITPEWMYGNNFASYASWVTFTTLGNLIGGLIPNPEQ
                     FGLDFALVAMFVGIFAGQLEAMVRTIPLKKIGLILLAVGLTYIGLSMMVSSYVAVLLA
                     TLLGCFVGVMIDDKN"
     gene            complement(19084..21285)
                     /locus_tag="ACHU66_000775"
     CDS             complement(19084..21285)
                     /locus_tag="ACHU66_000775"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR3389074.1"
                     /translation="MGIIFHEKTKEFHLYNEFISYVLTVLPNGHIGSLYYGKRVTETA
                     SYQYVREDEYRALTSFVEDDDRFFSLQYANPEFACYGTTDYFSPTFELVQKDGSSLSH
                     FVYQGHVIYAGRSALKGLPHLYLDEEGQADSLDIYLLDEESQTRLVLSYTIFRDYPAV
                     TRSARFEQLGEQSVCLNRALSMTLCLPDMDYDWLHLDGAWGRERHLQLSPLHQGCQSI
                     YSLKGASSAEHNPFMALKRPTADEQQGEVLGFSLVYSGNFLAQVDVSSFQKTRVSMGI
                     HPERFSWTLDKGEEFQTPEVVMVYSEHGLNGMSQTYHRLFQKHLVRGYWRDRERPVLL
                     NNWEAMSFDFDEETILSLAKEAAGLGVELFVMDDGWFGKRNHDRAGLGDWTVNREKLP
                     SGLTGIIDQVHAMGMKFGLWIEPEMVNKDSDLYRAHPDWIFHHPQHSQSHGRHQYTLD
                     LSREDVYQNIHDQLHRLLAEHDIDYIKWDMNRYMTEVFTCTREAERQGETFHRYILNL
                     YRLYDSLIAAFPTVLFESCSSGGARFDPGMLYYAPQTWVSDDTDAMERLKIQYGTSMV
                     YPLNSTGCHVSASPNQQLGRATPLSTRANVAFFGSFGYELDLFECSQEELEEMKEQIA
                     FYKAHRKLFQQGIFTRLKSPFDGEITAWQVQSLDGQQVIVGYYRRLTTANLSYQRLYL
                     QGLEEEAVYHLDGEVYTGSQLMHTGLSIRHGDHVGDNKDFTSYLMVLQKQQ"
     gene            complement(21295..22125)
                     /locus_tag="ACHU66_000776"
     CDS             complement(21295..22125)
                     /locus_tag="ACHU66_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR3389075.1"
                     /translation="MKQSAFKSTIWMHAFLILVAMGMLIPFVWMVLTSFKTVTESTQM
                     NPFHFLPSNWMVSNYTEAIRTNNFPILYLNTILMMLWRIFSSVMFSAMAAYAFARLEF
                     PGRNFLFGLVLFQMMVPPQLFVIPQYLMIDQLGMRNTIFALVFPGIVSAFGTFLLRQF
                     FMGLPKELEESAKLDGCNIGQTFFKVMLPLAKSGLIALAIFTALFAFKDLLWPLIINS
                     EADKATLSSALSKIQGAYAVNYPQLMAASVLAIWPMLVLYVVFQKQFIQGIATSGGKL
                     "
     gene            complement(22136..23029)
                     /locus_tag="ACHU66_000777"
     CDS             complement(22136..23029)
                     /locus_tag="ACHU66_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR3389076.1"
                     /translation="MFRKKGSLNEAIWGWGMVAPTIIGLIVLNIIPIFQTMKMSFHKS
                     GDFGKGDIFVGLANYQRMLGDAQVWQATWNTLKYTILVVPATVALAMLLAVLLNSKIK
                     GKHIYRTIFFLPMVAAPAAVTMVWKWLYNTDFGLINYILRRLGLGAVNWIEDPKIALY
                     SIALIGIWSTVGYSMILILAGLQEIPTDFYEAARIDGASPAKQFFSITLPLVSPTLFF
                     VVVTSVIQSMQVFDVIYMMEDVRSPAYDKTVSLVYLFYNNSFKYSDKGYGSTIVMLLL
                     LIILVITFVQMKVQKKWVHYR"
     gene            complement(23098..24372)
                     /locus_tag="ACHU66_000778"
     CDS             complement(23098..24372)
                     /locus_tag="ACHU66_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFR3389077.1"
                     /translation="MKKIGRYVTLLAAAGLLAACSTSTESSKTTEGSSSGKTEISYAI
                     WDSGQEPGLRKIADEFEKKNPDIKINIQVSDWDSYWTMLEAGATGGSLPDTFWMHSNE
                     IYRYGSNEMLLPLDEYLAKSEDAKLANFPDGLNEIYNINGKQYAIPKDFDTIGLWYNK
                     KLFDEAGIPYPDDTWDWNKLKEVAKKLTKPDGSQYGFGAGLSNQEGYYNFIYQNGGKV
                     ITDDLKSGYADPKTIEALDYYFSFVKEKISPAITVDKERAEAFQNGQVAMSVFGSWNL
                     SGFTANDYIRENADVAVLPKGPDGTRATIFNGLGHAIAATTKHPDAAWKWVEYLSSKE
                     AQEMQATLGVAISAYKGAADTWVDSNKNFTIKNYVDMVDYAQIRPYSQTTIKWEDKAY
                     ELLKPAYLGEKATEEAAKETADMMNAELATEK"
BASE COUNT         7271 a         5835 c         4608 g         6752 t
ORIGIN      
        1 atattcgagt gactagatgt gttttttatg tgtttaaatt ctactgaaag actctgtttc
       61 ttcatcgttg aatagatgtg actatttttc caatggatgt ataatcacac cctgcaccac
      121 acggttgacg gtgcctgttg gtgatggtgt atctggcaag ttgttgcact taatggctgc
      181 catgatggat accgtttggg caaagaggat gtaagggaag gttaggaaga catctggcag
      241 atctgctccg ccttctgcca agacaaagtt ggctgcttct gtaccgacta acttatcagc
      301 cgacaatgcc actatccgct ctgctatgcc atcaccatga acttcattaa gcaagtccac
      361 atcatactga cgagtgtagg catcgtttga ggcaaagagg aaaatcaagg tcttttcatt
      421 gatgaaggat tttggtccat gacggaaacc gagtggtgac tcgtacatgg tcgcaatctt
      481 acctgctgtc aattccagaa ttttcaactg agcttcgtgg gcaagaccat agaaaccacc
      541 tgaaccgagg taaatcactc gctcaaaatc aagattaatc aagtcttgca cgtagtcttc
      601 acggtccaaa acatcttgac ccagacgggc aatgatctca acccaagcag ctttttcttc
      661 ttgactggct ggcgagaaga cgaggagggc tgtcagagcc atacagctgt aagaacctgt
      721 catggcaaag cccttatcgt ttgaaccagc tggttgcaag agtaggagat tttcaacatc
      781 tccttcagcc gctagtgcca gcttgccttc tggtgcacag gtaatggtca cttgatacaa
      841 tttatctacc aattgcttgg cgacttcaac tgctgccaca ctttctggtg aattgcctga
      901 acgggcaaag gacaccagga tggttggaag atcagcttgt agatagtcat gtggacgact
      961 aaccagctcc actgtaccaa tggcagaaaa acgaatgtgt ttcaagtcat tgacttggtt
     1021 gaggtagttg gcaatacttt gcccaacaaa gtcagaagtc cctgcacctg taaaaacaac
     1081 gtgaacaaag tcatgttttt ccttgattga ttttagaaaa ctctcgattt tttccaattg
     1141 gtcaaaatag agttgatgcg cttccttcca taagcctggc tgttggtaaa tttcggtcgc
     1201 cgtaatcata gctcccagct tttcaagttc ttcttttgct aaacggaaca tagctcttcc
     1261 tccgtgattt ctggttataa ccagtttttt cttatattat agcgaattta cccctcaaag
     1321 tcaatttaaa atagattaat atgtcaaatt tatatccgat attgccgact agaacttatt
     1381 ttgagaaggt agcattatct acataaaaca tgttataata tttactacac ctctgccttt
     1441 gaaaattttt aacaataagg caaaataagg caaggaaaaa ttacaaacaa ggtctaaacc
     1501 cttgatacga aaggaatctt aaaaatctta tgatgaatat gcaaaacatg atgcgccaag
     1561 ctcaaaaact tcaaaaacaa atggagaaga gccaagctga gttggctgct gctcaattta
     1621 ccggtagctc tgtacaggac ttggttactg ctacttttac tggcgacaag aaattggttt
     1681 ctattgactt caaggctgac gttgtagatg cggatgatat tgaaactctg caggaaatga
     1741 ccgtccaagc cgtcaacgct gcacttacaa aagtcgacga ggcgactcag aaaaaactag
     1801 gtgcctttgc aggtaaattg cctttttaaa aagaaaacgc gattggatac tctcctctcg
     1861 cgattttttt attcaaaatt ataaatcaaa gactgaaatt gctggaaaaa gaggtctgga
     1921 tagcgtcgat tctctttcat ttcctccatc agactttgga ggtcggtcat gttgtcagtg
     1981 atgataccgt ccacttgtag cagcaacatc ttggtcatac tctcttcttc attcggtgtc
     2041 caagcataaa ccgacttccc tcgaatccaa gacttggtca taaaattttg atccagcgaa
     2101 gtgtattcca ttgtataacc atcagcaacc gtcgtcggat aaattgaatt gaacggaaga
     2161 ataaagaagg atactaattt ctcgctatac tgctttattt cgataatagt acggtaatct
     2221 aatgattgca tctggtgtcc tttggcaatc aaacgttgac cgtatttttt taagaaattc
     2281 tgggtgagtt ttggcgaatc tgccttggtc actttaattt ctaccaataa tttctgtccc
     2341 agctcatccg ctttttcaag atattcatca aaagaaggaa ctggagctac catgccgttt
     2401 tctgatgcag tcatccctgt caactctgca agagtatagt catgtgtacc tccgggattg
     2461 cccgtcaaag ccaataaatc cgtatcatgc atgacaataa actgaccatc cttcgtctcc
     2521 tgaacatcca tctcaatata atctggcttg agctgagagg ttttctctag ggcctcgata
     2581 gagttctgga cagcattgcc gtcatcgaca cctcggtgag aaatggtaac cggcagagtg
     2641 tcaaagggaa agtagagggc aagcgctcct tgggtaccga acaccagacc agatacgagc
     2701 aaaatggcta gtcgaaggtg atgacggagg cgtttacggc ggcagattgg tagttgtttt
     2761 cctgttagga ggctgacaaa tcccatcatg aagagtgcaa ctactccgta gtaggctaat
     2821 tttagaagtg cgtaacagac aactgccagc cagtaagaag cacttggtac aaaatgctct
     2881 gctaacagtt gcacaagata gaatagtcct cctactcctg taaataggag aactggaaaa
     2941 gtaatcaacc aaattagacg gaacaatgaa ccgatctgct ttctgcctct ggtcttctcc
     3001 caagaataag cgatagactc cttcacaccc ctgtgttcaa aataaatgtt tggcagagcg
     3061 tacatgaagc gtgccgcaag ccagaagaac aagaggagga aaatcaaaat caatattcct
     3121 agccagactg tgttggatag gtaatctacg ataaattggg gcactacgat tttattaaaa
     3181 taatagatat ttaatactcg gcgtaaaaat gggagaagaa taactgagta aaagagcaga
     3241 aatagaacct ttggtaggct gctgtgacgg ataatcgcca ccatactatc tcgtacatcc
     3301 cgtaaatagt cggggaggtg cttgactttt tcatccaaca gttgccaaac ccctgtgaac
     3361 aatagagcta attctagata ggcaattgca atgttgacaa aaaagaggag aataaacaag
     3421 cctgtcacga gtagattttc agcaaacacc gacaacaaat tcgtcggtga aaggtaggca
     3481 tgccctgtca tagctaagag tccctcagaa atccatgaat tgagcggtat ccaaacaaat
     3541 tccataaagg taaacagggt aaagaagagc aagagaatct tgtctaggtt gtaatatatt
     3601 ttttgaaatt cacgttgtat ctttttcatg attctagtgt cccatattct tgcaagaatt
     3661 gcaagccttc atcaaggatg gaagattgtg aaagcaatga atgaagtata gaaaaaatca
     3721 agttcctaag tggagcttga tttcttacat ttgatttgct ttcctatttc caagccaaag
     3781 taaatcgaaa ggctacataa aatacgaatc actatctcac tattgttgta acattaattg
     3841 tgcttgttta acaatatatt ctgctgtcaa accatataca tccattaagt aagcttgttg
     3901 cccaacttga ccaaaagatt cctcaatacc aattcgatgt actggggtcg ttaaatctgt
     3961 agccattaat tcacaaatgc tactacctag gccaccaatt cgattatgat tttctacagt
     4021 taatactggc cttcctgata atagagctgg tatgtcttca tgaattggtt tgatacgaaa
     4081 aagatcaatg acttgaacag atactccttg agcctctaac tgatccgccg cctttaatgc
     4141 ctgctctacc ataataccac tcgcaagcaa ggtcatatcc tccccttgcc taaggacaca
     4201 gtagcctttg ctaaaatctt ctccaccttg ataaatagcc gttgtcgctt tccgaatggt
     4261 acgaatgtac ttcaacccgt ctaccttgat ggtttcttta agaacagctt gaaactggac
     4321 atcgtcactg acctcataaa cacgcgcttt tggaatgagc cgcattaagc ccagctcctc
     4381 aaacggcatg tgagtcccac cattcatctc cgctgatacc cccgcatcgg aaccaatgac
     4441 cgtggcatta agttgggcat aagctagaga gataaacacc tgatcataga ctctacgact
     4501 agcaaatggt ccgaaggtat gaatgtatgg cttgtatcct agaagagata atccagctgc
     4561 tacccctacc atttcctgct ccatgatacc tacattgaca tagcgtccac ccagaacaga
     4621 ggacaatttg ttggtagcca ttgaactgga taaatctgct tcaatagctg ctatggttgt
     4681 gtcttcctgc cctacctgag ctagaaaatc actataaacc agtcgcatct cctttgtctg
     4741 tctcattcca tcacctccaa ctctctcgct aaaacaagag ttacttgctc taaatcttcc
     4801 cgtaattgac ccgacaaacg caaatggtga ttgctttcta attcttctaa gaaggtaact
     4861 ccctgcccct tagtagtatc aagaacgata cattttggtt gaggagactt acttgacttc
     4921 attgttagaa tagcatttat aatagcatct atatcggcac cattcacacg cactgattcc
     4981 caaccaaaag cctgaaattt ttcaacaaaa tcaaaggttt ggcaaatatc atgggtacga
     5041 ccgtctagtt gttttttatt gtcatccaca aacagaatca ttttcgatag ttcgtggtga
     5101 gctgcaaact gagctgcttc ccagcattga ccttcgttaa gctccccatc ccctaccaga
     5161 gtataggtat agtacggtga ctgttctatc ttcttaccat aagcaacacc tgtcgcgaca
     5221 ctcatccctt gccccaacga acccgttgtc atatccaccc ctggcgttaa gtttcggtca
     5281 gggtgtgaag gcaaatgtgt tccattttga tttagcgaca agagaaaatc tctatcaaag
     5341 aagcctttca aatacaacgt actataaagt gctggacctg catgtccctt agacagaata
     5401 aagtgatccc gctccttaga agagaaattc tggacacgaa tgtccagaat ttctccatat
     5461 agagcagcca acacttctac gattgataag ctaccaccat aatgaccgaa tcctaattga
     5521 ttcaaagtcg ccaaggtatg gtagcgaatt tctttggcaa atcgtttcaa atcagctgta
     5581 ccactagtca tattatcctt ctttctgttc ttttttcacc aatgacaatc caagcatcaa
     5641 agccaagaca gcaaagatac caaccgaaac tgtgaggata ccgccattct tagctagcac
     5701 tcccaggaaa ataccagaaa gaccaaagtc tgcatctgag aaagttgaac cagcaaaacc
     5761 aaggtctccc aaaacaggaa gtaagaatac aggtaagaaa ctaatgagaa ccccttgcat
     5821 aaaagcacca caaactgcgc cacgaacacc acctgacgca tttcccatga cccctgcagt
     5881 tgctccacag aagaagtgtg gtacgacacc tggaaggata acgacaccgc cagtcaaggc
     5941 aagaataatc atgctgacaa taccacctgc aaaactagag ataaagccaa tcaacacggc
     6001 gtttggtgca taaggataaa caactggaca gtctagggct ggtttggaat tcggaaccaa
     6061 cttctctgaa attcctttga aggctggaac aatttccgct aaaattaagc gtacaccaga
     6121 taagataaca aaaacacctg ctgcaaaagt tccacctagg gtcaaggcgt aaatcatgct
     6181 gttagtacca ccactaaggt tttccttaat ataatcaccg cctgcaaaca aagccacaat
     6241 catgtaaatc aaggacatag aaatggcaat gctgacagtt gaatcacgca agaatgccaa
     6301 acctttaggg aaattaatat cctcagtaga cttagactta tcaccgacca aaccgcctac
     6361 tacaccactc aaccagtaac caagtgcact gaagtgacct agggcgacat gatcatttcc
     6421 tgtcaattga atcatgtatt tttgaacgaa ggctggggag agtgtcataa tcagacctaa
     6481 agccaaccca cccatcacaa ttaggccgac agaagtgaat cctgcaacac tcatgataac
     6541 tgcaatcata caggccatat atagtgtgtg gtgtcctgtc aagaaaatat atttaaagcg
     6601 tgtaaagcga gcaatagcaa tattgaagac cattccaagc aacataatca aggatgtagc
     6661 agttccatac ttatccaaag ccatcgctac aatagcttca ttatttggaa caaccccttt
     6721 caagttgaaa gcttgttcaa acatttgacc aaatggctca agcgagccaa caataatatt
     6781 cgctccgcca ccaacaacaa taaaaccaac aaaagttttc aagcccccct taattaaatc
     6841 tgctgcgggc tttttctgta aggctaaacc tagaatagca atgagagcaa ccagaattgc
     6901 cggagtttta gcaatatcaa tgagaaaatc caataccatt ttctgtctcc tttataattt
     6961 tatctttgcg ggtcatcccc tgtgtatacg atttaaacca aacctttttc ttgacagata
     7021 gccgtgacaa gttccctcaa ctcattcata tcaataatac tattcaatat tcgaacatct
     7081 cctaaatgag ttgcagaatc agccaggtca cgtccgacaa tccacacatc tgcttcactc
     7141 ggattggcac ctcctagatc ataatgctcc acatgaacat ctgatacacc taaatctcgc
     7201 aaaatggatt caatattcat ttgaaccata aagctagagc ctaaacctga accacaggct
     7261 gttccaattc gtaacatatt actgttcctc ctttaataaa tgttgtacgt cggcaaattc
     7321 tttggcgccg acaagttgtt gaatattttc ttctgctcgc aaaatttttg tcaaatgcga
     7381 gagtgctctg agatgggttt gattatccac ggctgcaata caaatgaaca gccgaatttc
     7441 atgactaggg tcatccaata gataaactgg cttatccaat accaacattg acatccctac
     7501 ctcattaacc ccatcttctg gtcgtgcatg tggaatcgca attcctttac ctaggtcaat
     7561 aaaaggacca aactcttcta ccttcgctat catagcatca atatatcgag tttctatagc
     7621 tcctttatct accagaggtc gtgcaaccag ggcaactgct ttttgccagt ctaaaacttc
     7681 ctcagaccat tgaaaggtct cttttgtaat caattcttct aacataggac tccttcccct
     7741 ttgctcatgg gagcgttgat tgagaaactg caccaattca tattttaaag cctgctcctg
     7801 atgaatggtt gcatgtttcc caacaagtga taataactgc tcaatctcaa tcggaaaata
     7861 agccgcattt gggaaatgct gattaaccaa gtgaaataat tcttttttat gattagaggt
     7921 catcagctgg gataccaaga aaaatggtag ttctatattc agcttgatag ttccaaatac
     7981 catgtcatag tctgtcatgt ctagcatctt ccaatcctgt aaggaatggg tatcggcaaa
     8041 atagatatta ggaaatagtt gccgtagatt ttcttttaca atcagcgaag agctcactcc
     8101 gtttgggcaa acaaccaagg cccgataacg atatgaacgt tgttgacctg cttcgatgta
     8161 gccaccaaag tggatgacaa aataagatac ctcactatct ggtatagtaa aacctagatg
     8221 ctcttccaaa ggtcgcaaag ctttttttac aagatagaac aagtcggcat gctcttcttt
     8281 gatggtttca gtatatgaat tgacatttac caaacgagat gttaaacggt agtaagcagg
     8341 aattaggtga cgttgcaaac cttcaatcat ttccgagcga tgttcaaaag caattaagga
     8401 caaacgttcc atctcttcca ctatttctac agtcaagtgg taaaatagat catccttttc
     8461 tccgctctca ccttctaagc atccttgcag taaatgtgac agatactctt gtgtcgcagc
     8521 atttaattct aatgtgctag tactttttaa acgtaataat ttccaaaggc gttgcagaaa
     8581 atcttttaca aaccctccct ttccttcaaa attccagtta atacggttgc caacacgcgc
     8641 ctgacggata aataaaaata tcaaaaaata caaatattca tccaatctct cttctaaagc
     8701 tgagacttta taataattgc aagcttgttg actcactatt ttcaattctt caataggatt
     8761 ttcctcaccc caagatttaa gaatataatc caaagcccac acaccaatcg aagactgcaa
     8821 acaattactg agtgcataaa gcgccaagcg atgtttatct tcttctcgac caattagatg
     8881 atagcctttc gccctcgtat attccaatcg tacttgaaag tccaagcaaa gttcctttac
     8941 attcttaata tccgtcaatg ttgtgttacg gctcacactc aataattctt ggtagtgaac
     9001 gtttgataca aattcttttc ggataaaggt ataaagatag ataagaagct gtctttcttc
     9061 ttgcgaaaga tagatctggc gattgcgaaa gagcgaaaat acactttctt tttgattgta
     9121 aagctccgta gaaataacaa actcgctatc tttgtattgt atctgcggga aggaatgttc
     9181 ttgcaacaat tgattgacta gttccaaatt agaatgtagc tgctcctttt caattcctgt
     9241 ttgaatacta agttcgtaca aactttcttt ccgagtcgca aacaattcca tcagaatcgc
     9301 catggtacga ttatcaacca tattttcctc cttgtcttaa ttgtatccct tttcatttct
     9361 gatttctaga gtctttgagc ccaattaata taataaaaaa tacaaattca attgtgctca
     9421 ctacttaaac taaaaaagat tggttcccac caatcttttt atatcacatg ccatcttcca
     9481 ttttttagtg cgggctgaaa cagtctatcc ctagactgta cctcgctttc ttatttccaa
     9541 gctcgggctg aaacagtcta tccccagact gtttcactcc cccggacctt tattcgctct
     9601 cttgctttca agctcatgat aaaaaggtcc cccggacctt tttatttccc aaacaaacgt
     9661 gcaaagaagc ctttagcttg cacttcttct gcctgtgctt taacttcgtc caattccaac
     9721 ttgagaactt ccttatcggc catagctttt agtgtcaatt gctgttgctg atccaactgt
     9781 ttatccttct cagcaatctg cacatcttta accttgagtt gttcatcttt ttcggcaagt
     9841 tgcttatctt tagcttttaa ttgactatat aagcggatga tttcagcatt tttctcgtca
     9901 actaaaattt ccatcaattc acgatgcttg acttcatcac taatcggctc atcttcaaaa
     9961 atggttgttt tgtagatttc ctccaactta atcaagccag cacgatttac aacggtcaca
    10021 cccttgtcat ttttatctac aaattcttca ggaagtgatt tgacacgatt attcatagcc
    10081 tgccggctca ctcctaaaat ttcagctaac tcactgactg ttttttcgat tcccataata
    10141 tcctcggtac ttacatttct catagtttgt atcattaaaa tattaccaca gaaccctatc
    10201 cctgtcaaat ctaggggaat tttcttacca agcattagtg aataatcaca aacctcaaaa
    10261 gcatcttatc tcaatgcaat gttatgcaga aacgaaatat acattctatc taataaaacc
    10321 atttttagca ctcacttgct tgaaccaata tccagatttt ttgacagtac gcttctgagt
    10381 ttgaaggtca agcgagacca aaccgtatcg gttcttatag gtatttaacc aagaccagca
    10441 gtcaataaaa gtccagacca tataaccctt acagttcgct ccttcatcaa tacctcgatg
    10501 gagctgaatc aaatgatctt ccatgaagtg aatctgatag tcatcctgaa tcatgccatt
    10561 ttcttcaaaa acatcctccc cttcaactcc catgccgttc tcagttacca accattcgat
    10621 attcccatag ttttcctgga tatttttagc aatgtcgtac agcccttctt cataaatttc
    10681 ccagcctcta tggggattga ttttcttgtc aggcttatca taaggctgga aatattggga
    10741 taggaaagta gcttctccgt tccatccatc ttctgaagga gcctgcacgc gcaggggctg
    10801 gtaataattc acccccagga agtcaaccct attttctttg ataatggtca attcctcctc
    10861 tgtggtttct ggacgtaaac cgtgtttttc aagaatgtca accagttccc tcgggtattg
    10921 tcccaaaaca gatggatcca aaaagcttct cgtttgaaat agctcagcaa tccgcgctgc
    10981 ctgtaaatcc tctggactct gacttcttgg ataggcagga gtaagattga gaacaatgga
    11041 aattttgtgg tatgggtgta gttggtgaca ggcccgaaca gccaaagaac tagccagttg
    11101 agtatgatac gctacgacca cagctgcctt tgcatccacc ttgcaaggat aatgatagct
    11161 tcctagatag ccacattcta ctggaacaat gggttcatta aaggtaatcc aatgatccac
    11221 cagatctcca aagagactga aacactggcg agcataagcc tcataggccc agacaatctc
    11281 cttattttcc cagccccctt tttcctgtaa gacataaggc aaatcgaaat gataaagatt
    11341 gaccaaaagc tttatcccac gctctcgaat acctgcaaat acagccctat aaaactccac
    11401 agcttgcgga ttgacctcgc ctatcccttc cggaatcaga cgtgaccact gtatagaggt
    11461 gcgaaaaata ctatgacctg tttccaccaa caaatccaaa tctgactgga aatgttcata
    11521 aaaagtcgat gtttgttcca gtcctatttg ctggtgaaag agctcaggtt cctgactgta
    11581 ccagtaatcc cagttattcc acccctttcc atcaccagct acgcttcctt cactttgagg
    11641 acctgaagtg gaggagcccc acaaaaaacc ttctggaaaa cgatactctc tttgctcagt
    11701 taccattctt ctacctactt tctttcatgc tacagacgga gttgttagaa cctggattca
    11761 caatgcagct tccacaaaat tactttgctt gtacatacag gctcctgata aaaactaatt
    11821 tcccttagtc taagtatact cccctaaccc gtcaatggtc ttccgcaatc tcaaggaaaa
    11881 actccacaaa tcatagcaaa gactcaaact actttcttga taaaaatatg atacaataaa
    11941 ggcatgaata cagtagatac cattattata ggagcgggcc ctgctggcat gatggccgcc
    12001 atttcttcca gcttttacgg caagaaaacc ctgcttcttg aaaaaaacaa gcgtctgggc
    12061 aagaaactgt ctggcacagg cggcggacgt tgcaatgtga ccaacaacgg cacactagaa
    12121 gacctgctgg ctggcattcc tggcaacggc cgctttcttt acagcgtctt ttcccagttt
    12181 gacaaccatg acatcatgaa tttctttcag gaaaatgggg tcaagctcaa ggtggaggac
    12241 cacggccgtg tctttccgac caccgaccgc tcccagacca ttatcaagtg cttggagatg
    12301 aaaatgttgg aaaacggcgt caatatccgc acagggactg aggtggtttc cgtccgtaag
    12361 attgatgacc tcttccatgt caagaccagc gaggaaacct ttacggcacc tcagctcatt
    12421 gtcaccaccg gtggcaagac ctatccctcc accggctcga ctggctttgg tcacgacatt
    12481 gcccgccact tcaagctgga agtcacggag attgaagccg cagaaagccc cgttctgacc
    12541 gacttccccc acaagaagct ccaaggcatt tccctggacg acgtcaccct gacctacggc
    12601 aagcacgtca tcacccacga cctgctcttt acccactttg gcctgtcagg gcccgctgct
    12661 cttcgcttgt ccagttttgt caaaggcggc gaaactgcat ttctcgatgc tctgccgacc
    12721 cattctgacc aagatctgtt tgagcattta gaagccaaca gggaaaaatc cgtcaagaat
    12781 gccctacgag aactcatgcc agaccgccta gccgactttt tcgcggagaa ctacgactgc
    12841 aaggtcaaac aggtctccca aaaagacctg actgatctgg tctcccttct caaagcccta
    12901 ccgatcaaga ttaccggcaa gatgtccttg gccaagtcct ttgtgaccaa gggaggcgtt
    12961 gacctcaagg aaatcaatcc aaaaacatta gagagcaaga aggtcccagg cctccacttt
    13021 gccggtgaag tcttggacat caacgcccat accggcggct ttaacatcac ctactgtctt
    13081 gcaactggtt gggtggctgg aagttcctac tcttcgtaaa tcaaactcag acgttgttga
    13141 cttgatttga tgagtgtaaa gctccagtgg agctttacag cctgctaccc gaaaataaaa
    13201 ggtagcagcg ttctatctac atctaatggc acgaactttg ttcgccttat ttccaacctc
    13261 caaaggttcc ccgaacctgt ggaggtagtc tgagttcgat tttctttgag tattgcactt
    13321 ttataaatcg ctcttcaagc tcaacctaaa ggagaaacca tgcctatcaa ttcattcggc
    13381 caaaagattg gtatgccctt accgcattat agtagcggtg agcgaccaag tctggagact
    13441 atacaaggac acacagtccg cttagaaaaa ctgagcctgc accatgcaga tgatctctat
    13501 cagttttatg gaccaagtgc caagaaaact gactggacct acttgcccct tgaacccttt
    13561 gaggacagag cgacatttca ggactatttc cagactcttt tgaactcgac cgacccctat
    13621 tattttgcca tccttgacca agcaaccaat caggcagtcg gaacctttgc actcatgcga
    13681 atagatacca accatcgtac cattgaagtt ggctgggtca tcttttctcc gcaactgcaa
    13741 cggacccgac aggcgacaga agctcactat cttctcatga cctatgtatt tgaagaactt
    13801 ggctatcgac gctacgaatg gaaatgcgac tcgctcaacc aagccagtcg agtcgctgcc
    13861 aaccgtctgg gctttaccta cgaaggcaac ttccgtcaag ccctagtcta taaggaacgc
    13921 aatcgggata ctgcctggtt ttctctatta gaccacgaat ggcctgaccg taagcgtgcc
    13981 cttgaaaact ggctaaagga tagtaacttc gatggccagg gtcagcaaaa acaaccccta
    14041 tcctctttct aaattcaagc aaaatatgct tgaatttttt tgttttttgt tgtatgataa
    14101 aaccataaca atataacaaa aggagattgg ctatgcaact gaaattatct tctgtttcca
    14161 agaaattcga ccataaaatc attttggaag atacttcctt taccttcgaa caaggcaaga
    14221 tttacggttt gctagggcgg aatggggctg gtaagacgac cttgttcaac tgcattgccc
    14281 gtaatctgac cttggatggt ggctcgattc agtttgtgaa ggatgaaatt gcctttgact
    14341 acgataatac ggacatcggt tttacccaga cctatccgca actacctgcc tttatgacgg
    14401 cttacgagtt tgtccgtttc tatatggata tccacaagga taaactcaaa tctcctcgta
    14461 gtccagaaga atggctaaac ttggtcggta tcggagaaga agaccggcac cgtctactca
    14521 aggacttttc ccacgggatg cagaacaagg tccagttgct tctgtctttg attgtccagc
    14581 cacctgtcct tctcttagat gagccgctga cttcctttga ccctgtggct gcccacgagt
    14641 tcaaacagct gattcgggag gctaaaaagg attctgttat catcttttcc acccatattc
    14701 tacaactggc tcaggatctc tgtgacgaga ttgttttact acatcaccaa gaactacagg
    14761 ctgttccgag tgacaagctc cacgaccctg attttgaaca ggaagtggtt accctcttga
    14821 cagcagacta ggagggactt atgaaaacca ttctacgtta tttttggtac caggaaaagt
    14881 ataacctcta tcggaccgtc aacggatttt tctactatct gcgaaaactg ccacttgtgg
    14941 gcaagagcat tccagagagt attttcaagt cttacagttt taagtctggt ctctttttga
    15001 tcctgcacgt cctctccatc cccagtcgct ttctggtcaa gggaatttgg ttagccctga
    15061 atttctactt ggcatccttt tggatcaatc tcctctctgg cggggatttg actttttgga
    15121 atattctacc agggacttgg cttttgggct tttctctctg gatggtcttt gttggctaca
    15181 cctatcggtt tggcaagggt tttgagccct ttatcgtcaa gtccgagcgg gaattcatgc
    15241 aaaattttgg actttcccag tctagttttc tccagagtca actatttgta gaacctatta
    15301 tcaccagtct gttctacctg cctgccctgc tgattttttc tggcctatca ggaaattggc
    15361 tctatctgcc tctgggatta ctgaccattc cgacaggaag ttttaccgga caggctctca
    15421 atcgttggtt ttttaatcga cggattctat cccgccgtaa ttcttggcaa tcctgggtta
    15481 tcttgggtac tggtctagca gcaattgcct gccttatcct tttccgcaat catctgtcgc
    15541 ccatcttttt actgcctgtg cttgtctgtc aggtcttgct catttggttt ggctatggtt
    15601 atttgaagca acaaaccaac catctagact atctctatta ttgcatggac cagtccttgc
    15661 agatggataa gaaaattttt gagatgacca aagggaacga gtacacccga caaggtttac
    15721 agatgcaggc gaaactgagt atggaaacag gaaaggacct gactcatctg tcaggcatga
    15781 cctacttaaa cgctctgcta tttgaccgct atcgttctgt ccttacgaaa aaactgcact
    15841 ttcgcttggg ttgtcttttg gctattgtcc tttttattga aggaattcgt tggttttcaa
    15901 aagatgacat tcaaataagc aataccaact atatcgaggg actgcctttt gctttcatgg
    15961 tcatgtatgc cgcctccatg ggtaaggctg tcgcacagat ggtctttgtt aattgcgata
    16021 tttccatgct ccactatcca ttttaccgcg aagcaaaaac cattattgca ggctttcatt
    16081 accgttttct gcaaactgta aaattaaatg ctgccttctc tggatctcta ctattggccc
    16141 tcatcatctt tactagagcg cagctaccta ttgaaaccta tctcctgact gctctgctcc
    16201 tgctgagtct aacagcccta ttttccttcc acgacctctt tatctactat attctccaac
    16261 cctttaccaa ggacatggag gttgtcaacc cagtctataa atttctcagt ggggctctct
    16321 actgggtggc ctatctcaat atcaagctgg atttaggcag tcacatgtac atcctactca
    16381 tttccatagc tatgattacc tatgtcagca tcggctactg gattttgttg aaaaaagctc
    16441 cgcagacttt ccgcttgaaa tcataaaaaa tagcctctcg caagagaagg ctgttttctc
    16501 gtcatttagt ttctctttta ttacttcgac gaatgaggaa actttgtttc cttatttcca
    16561 acttcaaaca ctccactgga gtgtttgaac tcgccttgcc tgattgaaac agtctggggg
    16621 actgtttcaa gtcaatgcct agaaataaga aagcattgag ttatgtctgc tgcttttgat
    16681 gtgaacatcg ttcactttat ccccagtctc caactatctc ccagatagtt ggagcgagta
    16741 ctaaaagtaa actaaaagac tatatttacc agatccccaa aattgcttgc caaaccagtg
    16801 tcaacgctgt taccccaaac cagcaaacta gtccgactag gagagccgct ctgccatttt
    16861 tcaccaaggc aaacacattt gtccgcaaac caatcgccac catagccata caaatgaaaa
    16921 ctttagagag ggacttcagc ggagcaaacc aatctgcccc tataccaaat ccacccgcta
    16981 tcgttgtgat tagactagct aagataaaat acaagataaa gaccggaaaa cctgccaaga
    17041 gggacttctt gtctgcttga actcccttcc ctcgggtttg ccaaattgct aagaccgttg
    17101 taatcgggat aatagctaaa gttcgtgtta acttaaccgt cactgctgta tctaaggtct
    17161 gactacctaa accatacaag ctatcccagg tcgaagccgc tgctgtcaca gaggaggtat
    17221 cattgaccgc cgttccagcg aacatcccaa agacctgacc cgaatcagtc gaaaacccaa
    17281 gccaagttgc caaagtagga aagaccaagg ctgccaagac attaaagaga aagatgaccg
    17341 agattgcttg tgccacatcc tcatcatctg cctgaataat gggagcagtc gccgcaatgg
    17401 cagagcctcc acagatagag gtacctacgc caatcaaggt tgccagatga gatgaaatgg
    17461 gcaagatttt ccacatcagg aatgccaaaa tcaaagcaaa actaatggtc gaaaaaataa
    17521 ttggaagaga ttgacgccca actgccaaga ccgctgataa gtttaatcca aatcccaaac
    17581 aaatcacagc atactgtaag attttcttag aaataaaggt taagcctgac tgaaatgcat
    17641 ttttacttga aaaataggga tacaggctca tcccaattag aagggcaaaa acaggcccac
    17701 ctacaagtgg aaataaatga cctaaccact gacccaccaa agccaaaagg aaacaaagtc
    17761 cgacaccttt ggcattttct tttaccataa aaacctccta aaatatcaaa cgtaacaacg
    17821 cggttgtaac aacgccaacc aagactgcta aaaggatatt ttttgttttc aaaaccacaa
    17881 aaaatgtcgg aagaatagct aagctttcca cagggagaaa acttggcaag gagccaactt
    17941 tctcatccat aatactggag agggtcaatg caaaaatgat agtgattggc aaaaagctga
    18001 ggaatttcac caactttgga gggagagatt tattttgcgt caggacaaaa ggcaggacgc
    18061 gtggcaccca ggtcaccagg gccgccatta gaataatcag taaaatgtta gtttttatca
    18121 tcaatcatca cccccacaaa gcaacctaat agtgtcgcca agagcaccgc cacataggac
    18181 gacaccatca tagacagacc gatataggtc agaccgactg ccagcagaat taagccaatt
    18241 tttttcagcg gaatggtccg aaccatagcc tctaactgac ctgcaaaaat accgacaaac
    18301 atggcaacca aggcaaagtc caatccaaac tgctcaggat ttggaatgag accaccaatc
    18361 agattgccga gagtcgtgaa tgtaacccaa gaggcgtagc ttgcaaaatt atttccgtac
    18421 atccattctg gagtaatggt atgatgctca atctgcttac gcaaaagaac tgcataggat
    18481 tcatccgtca caaaggatcc tatcaagata ttcgacccta aacgatttcc ttgaaaaata
    18541 gttgtcgcat gcaagcacat cagaaaattt cgcaggttga caaagaaaac ggttactgca
    18601 atggataaca aaggcgcacc tgcaaaaatc atagcacaca taacaaattg agcactcccg
    18661 gcatagatta ataggctcat cagccccatc tccacagtag aaatgcccga attaaccgat
    18721 acgactccgc aggctagacc aatactggca tagcccaata cagtgggaat ggcatcgcgc
    18781 atcccttcac gaaaagcata tttcttcatt cattatctcc cttcctctca ctaattgatt
    18841 gaagaattat tctctcattt tctcacattg caacccgttt ttcaagaatt cttatatcta
    18901 atactcaatg aaaatcaaaa gtagcctagg aaacgaagcc gaagatagaa ctggagttca
    18961 tcaaggcaag ttgacaacgg ataattttga ttttcgaaga gtataagtag aggtcttgcc
    19021 ctcattgatg tactttctgc ataaaataga cccagccgat agctgggcct agggataggg
    19081 atgctactgt tgtttctgaa ggaccatcaa ataagaagta aagtccttat tgtctccaac
    19141 atggtctcca tgtcgaatcg acaaacctgt atgcattaat tggctaccag tataaacttc
    19201 tccatcaagg tgatagaccg cttcctcttc caatccctgc aaatacaagc gttgatagga
    19261 gagattagcc gttgttaacc gacggtaata acccacgatg acttgctgac catccaagga
    19321 ttggacctgc caagccgtta tttctccatc gaatggactt ttcagccgag taaaaatacc
    19381 ctgttggaaa agcttacggt gagccttgta aaaggcaatc tgctccttca tctcttccaa
    19441 ctcttcttgg ctacactcaa acaaatccaa ctcatagcca aaacttccaa agaatgcaac
    19501 attggcacgt gtgcttagtg gagtagcacg ccccaactgc tgattcggac tagctgacac
    19561 atgacaaccc gtgctattta gaggatatac catgctcgtt ccatattgga ttttcaagcg
    19621 ttccatggcg tccgtatcat cactgaccca ggtttgcggt gcatagtaga gcattcccgg
    19681 gtcaaatcta gctccaccac ttgaacaaga ttcaaacaga accgtcggaa aagccgctat
    19741 caggctatca tataaacggt agagattgag aatatagcga tggaaggtct ccccttgccg
    19801 ctccgcctcc cttgtgcagg taaatacctc tgtcatatag cggttcatgt cccacttgat
    19861 ataatcaata tcatgctcag ccaacagccg atggagttgg tcatgaatat tttgataaac
    19921 atcctccctt gacaaatcca acgtatattg gtggcgtccg tgagattgac tatgctgagg
    19981 atgatggaaa atccagtctg gatgcgctcg gtataaatcg ctgtctttat taaccatttc
    20041 aggttcaatc caaagtccaa atttcattcc catagcatga acttggtcaa taatccctgt
    20101 caagccactt ggtaatttct ctcggttgac cgtccaatct cccaagcctg ctcggtcatg
    20161 gtttcgcttg ccaaaccaac catcatccat gacaaagagt tcaacaccaa gacctgcagc
    20221 ctctttggcc aaggaaagaa ttgtttcctc atcaaaatca aaactcatgg cttcccagtt
    20281 attcaagaga acaggtcgct ctctatcacg ccaatagcca cgtaccagat gtttttggaa
    20341 gaggcggtga taggtttggc tcatgccatt caaaccatgc tcgctataaa ccataacaac
    20401 ctcaggcgtc tggaactctt ctcccttatc taatgtccaa gaaaaacgct caggatggat
    20461 tcccatactc actcttgttt tttggaaaga actcacatcc acctgcgcca aaaagtttcc
    20521 gctatagacg agcgagaagc ctagtacctc tccctgttgc tcatccgcag ttgggcgttt
    20581 caaagccata aagggattgt gctccgcact actagcccct ttgagactgt aaatagactg
    20641 acaaccttga tgaagaggcg atagttgcag atgccgctca cgcccccaag ccccatccaa
    20701 atgcaaccaa tcatagtcca tatcaggcag acaaagtgtc atactcaagg cacggttcaa
    20761 acagacagat tgttctccga gttgttcaaa acgagcagag cgagtcaccg ctggataatc
    20821 tcgaaaaatc gtataagaca agaccaggcg ggtctgcgac tcttcatcca acaaataaat
    20881 atccaaacta tctgcctgtc cctcctcatc cagatagaga tgaggaagac ccttcaaagc
    20941 cgatcgccca gcataaatca catgaccttg atacacaaag tgcgacaggc tactaccgtc
    21001 cttctgcacc aactcaaaag tcggagaaaa atagtccgtt gtaccataac aagcaaattc
    21061 aggattggca tattggagcg aaaagaaacg gtcatcgtct tctacaaagg aagtcaaagc
    21121 acgatactca tcttcacgca catactgata ggaagctgtt tctgttacac gctttccata
    21181 atacaaacta ccaatatgac cattaggcaa gaccgtcaac acatagctaa taaactcatt
    21241 gtaaagatgg aattcctttg tcttttcatg aaagataatt cccattgtag cctcctataa
    21301 ttttccacca gatgtcgcaa tcccttggat gaactgcttc tggaatacca catacagtac
    21361 caacatcggc caaatagcca aaacacttgc agccatgagc tgaggatagt taacagcgta
    21421 agctccctga atcttagaaa gtgcgctaga cagggtagcc ttgtccgctt ctgagttgat
    21481 aatcaatggc cacagcaaat ccttgaaagc aaacaaagcc gtaaaaatcg ctaaggcaat
    21541 caagccagac ttggccaaag gcaacatgac cttaaagaag gtctgcccga tattacaacc
    21601 atccaatttt gcagattcct ccaattcttt tggaagaccc atgaaaaatt gacggagcaa
    21661 gaatgtccca aaggcactga caattcctgg gaataccaga gcaaaaatcg tattgcgcat
    21721 accaagttgg tcaatcatca aatactgagg aatgacgaat aactgaggtg gcaccatcat
    21781 ttgaaagagg accagaccaa acaagaaatt ccgcccagga aactccagtc gagcaaaggc
    21841 ataagctgcc atggctgaga acatgaccga gctaaaaata cgccataaca tcatcaaaat
    21901 ggtgttcaga taaagtatcg ggaaattatt agtccgaata gcctccgtat aattgctcac
    21961 catccagtta cttggtaaaa agtggaaagg attcatctga gtggattcgg ttaccgtttt
    22021 aaaagacgtc aacaccatcc aaacaaatgg gatcagcatg cccattgcca ctaaaatcaa
    22081 gaaggcatgc atccagattg ttgatttaaa agctgattgt ttcacatgaa cctccttaac
    22141 ggtaatgcac ccattttttc tgtaccttca tctgaacgaa agtaatgaca agaataatta
    22201 ataacaacaa catgacgatg gtggagccat agcccttatc cgaatattta aaggaattat
    22261 tgtaaaacag ataaaccaga gatactgtct tatcataggc aggactacga acatcctcca
    22321 tcatatagat aacatcaaag acttgcatgg attgaatgac actggttacc acaacaaaga
    22381 acagggttgg tgaaaccaag ggcagggtaa tggagaaaaa ttgcttggcc ggactagctc
    22441 catcaatgcg agctgcctca taaaaatccg ttggaatttc ttgcaaacca gctaaaatta
    22501 aaatcatact ataaccaacc gtactccaaa ttccaatcaa agcgattgag tatagagcaa
    22561 tctttggatc ttcaatccag ttcacagcac ccagtcccaa acgacgcaag atatagttaa
    22621 tcaagccgaa gtccgtattg tagagccatt tccaaaccat ggtcactgcg gctggtgctg
    22681 ccaccatagg caagaagaaa atggttcgat aaatatgctt acctttaatc ttagaattaa
    22741 gcaaaactgc aagcaacatg gccaaggcaa cggtagctgg aacaactaga atcgtgtact
    22801 tcaaggtatt ccatgtcgcc tgccacactt gcgcatcccc taacattcgt tggtaatttg
    22861 ccaagccgac aaaaatatcg cctttaccga aatctccact cttatgaaaa ctcatcttca
    22921 tggtttggaa aataggaata atattgagca cgattaatcc aataatggtc ggtgctacca
    22981 ttccccatcc ccaaattgcc tcatttaaac ttcctttttt tcggaacatc ttatccccat
    23041 ccttcttcat tttaataaga aggtccgggg aagaatcaac ttcccaagac ctcctaccta
    23101 tttttcagtt gctaattcag cattcatcat atcagctgtt tctttggcag cttcttcagt
    23161 tgctttttct ccaagataag caggtttcaa tagctcataa gccttatctt cccacttgat
    23221 ggttgtttgt gaatatggac gaatctgtgc ataatccacc atgtcaacat aattcttgat
    23281 agtaaagttt ttgtttgaat ctacccaagt atctgccgcg cctttataag ccgagattgc
    23341 cacaccaagt gttgcctgca tttcttgtgc ctcttttgag ctgaggtatt caacccattt
    23401 ccaagcagca tctggatgct tagtggtagc agcaatggca tgacccaatc cattaaagat
    23461 ggttgcacga gttccatctg gaccttttgg caagacagct acatcagcat tttcacgaat
    23521 gtagtcatta gcagtgaaac cagataaatt ccaagaaccg aagacgctca tagccacttg
    23581 accgttttgg aatgcttcgg cccgctcttt atctactgta atagccggtg aaattttttc
    23641 tttgacaaag ctaaagtagt aatccaaagc ctcaatggtc ttaggatcag catagcctga
    23701 tttcaaatca tcagtaataa ccttaccacc attttgatag ataaagttgt agtagccctc
    23761 ttggttactc aaaccagcac cgaaaccata ttgactacca tctggcttgg tcaatttctt
    23821 cgctacttct ttcaacttat tccaatccca agtatcatct ggatatggaa tacctgcttc
    23881 gtcaaataat tttttattgt accaaagacc gattgtatcg aaatcttttg gaatagcgta
    23941 ctgcttacca tttatgttgt agatttcatt caaaccatct gggaaattag ctagtttagc
    24001 atcttcactc ttggctaagt attcatccaa tggcaatagc atttcattgg aaccatagcg
    24061 ataaatctca tttgaatgca tccagaaggt atcaggcaat gaaccacctg tcgcaccagc
    24121 ttctaacatg gtccagtagg aatcccagtc cgatacttga atattgattt taatatctgg
    24181 attctttttc tcaaactcat ccgcaatctt tctcaaacca ggctcttgac cagaatccca
    24241 aatagcatag gatatttctg tctttcctga actacttcct tccgtagttt tgctagactc
    24301 agtcgaagta gaacacgctg ccagcaggcc tgcagccgca agcaaggtaa catagcgacc
    24361 aattttcttc atgataatac tccttttctt ttgtcgaata tgtcgacgaa ctttgttatt
    24421 tctactataa ctataacatg taagcgcttt ataatcaaat aggaat
//
LOCUS       DAZQCJ010000021        24230 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000021 DAZQCJ010000000
VERSION     DAZQCJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24230
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.021"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            14..325
                     /locus_tag="ACHU66_000779"
     CDS             14..325
                     /locus_tag="ACHU66_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR3389078.1"
                     /translation="MKRNKHLPLTETTYYILLALLEPAHGYHIMQKVEDMSDGDVKIA
                     AGTMYGAIENLLKLKWICSVPSSDKRRKVYQVTATGQEIIKLETQRIQKLHHIAKELG
                     F"
     gene            336..962
                     /locus_tag="ACHU66_000780"
     CDS             336..962
                     /locus_tag="ACHU66_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HFR3389079.1"
                     /translation="MIRHKLFTSLQEEEHWINSIQSEGYQLVKVTPWTAAYHFEKCSR
                     TPHPVRLDFHEHIAKGEYSNYLSLFEDCGWQPIQGSRRCGIHYFQQTIEASSTEIFSD
                     TESKKAFYSRYQNYAYSYFGLFLTLFFIHYQVGLQNGYTLWNPKSWYLTPELWERTGT
                     SFWFGFLFETPFALFRSGIIPLFFLAWSIYFLHIAEKGKKEAKKLEKY"
     gene            complement(1034..3202)
                     /locus_tag="ACHU66_000781"
     CDS             complement(1034..3202)
                     /locus_tag="ACHU66_000781"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFR3389080.1"
                     /translation="MKKFLSFEFWQKFGKCLMVVIAVMPAAGLMVSIGNSIPLISPES
                     ELLIRIGNIIAQIGWGIIGNLHLLFALAIGGSWAKEKAGGAFSAGLAFILINLITGHF
                     FGVTTDMLADAAATVTTVFGTEIPVSGYFVNILGQPALNMGVFVGIIAGFVGATTYNK
                     YYNYRKLPDVLTFFNGKRFVPFVVIYRSVLVALGLAIFWPVVQTGINSFGKWIASSQD
                     TAPILAPFVYGTLERLLLPFGLHHMLTIPMNYTSLGGTYDILTGAQAGAQVFGQDPLW
                     LAWITDLINLKDAGDMAQYNDLLANITPARFKVGQMIGSSGILMGLTYAMYRNVDEDK
                     KHKYKGMFISSALAVFLTGVTEPIEFMFMFAAMPLYVVYAFVQGAAFAMADVVNLRMH
                     SFGNIEFLTRTPMAIKAGIGMDVINFIWVTALFAVGMYFIANFMIKKFNLATAGRNGN
                     YDTETTDVVSNSNVDTADANSQVVQIINLLGGRDNIADVDACMTRLRVSVKDVAQVGD
                     ENAWKQAGAMGLIIKDSGVQAVYGPKADVLKSDIQDLLESGVAIPRTEIVATEEVAPE
                     TQFKGVTETVYSVAEGQAIAITEVKDPVFSQKMMGDGYAVEPSSGNVYAPVSGIVTSV
                     FPTKHAVGILSDKGVEVLVHVGLDTVALNGAPFSAKVTDGQRVEAGDLLLVADLDAIR
                     SAGRETTIVVAFTNTAEIKSVSLGNLGQVSQDSQVATVEL"
     gene            complement(3236..4027)
                     /locus_tag="ACHU66_000782"
     CDS             complement(3236..4027)
                     /locus_tag="ACHU66_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR3389081.1"
                     /translation="MKLLTINVHAWLEERQDEKLDVLAKTIAEKAYDVIALQEVNQLI
                     TADLVTKDLKADNYGLILLEKLRALGQFDYSYYWSNSHIGYDKYEEGIAFLTKLPVYE
                     VDPFYCSQNKSVDSILSRKIMGLTVFYQDQLIDLYSCHINLPGSEEEDQLDNVRTIVE
                     RTGNRRFKILMGDFNTDAFSDREAYEAITELGLYDSYNLAMEKDQGITVEKAIDGWAG
                     HSQEKRLDYIFLNQKKEVLSSLVIFNGENRPIISDHFGVEVEINV"
     gene            4226..4933
                     /locus_tag="ACHU66_000783"
     CDS             4226..4933
                     /locus_tag="ACHU66_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HFR3389082.1"
                     /translation="MSKYKKVYQDIKKKIEEQVWSTGQALPTENELMEVYSYSKDTIR
                     KALSLLEMDGYIQKKQGKSSIVIEHGLMKDQYLSEIKTAGELNKRSQHQIQTQLTSLY
                     IIQGQEDLMSTFEVDDKVDFYRVSRVRTIDGERLEYDISYFDRRVVPYLSKEIAESSI
                     YRYLEEELHLTISHSRREISFRFANEEEKQLMDLGDYDMVVVVTSITYLSNGQAFQYG
                     TISYRPDKVSFVSMAKR"
     gene            complement(5160..7424)
                     /gene="glgP"
                     /locus_tag="ACHU66_000784"
     CDS             complement(5160..7424)
                     /gene="glgP"
                     /locus_tag="ACHU66_000784"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HFR3389083.1"
                     /translation="MTKFTTFAETNTSKKLADLTNEEIYLQLLNYVKDAAASKPKNTG
                     KRKVYYISAEFLIGKLLSNNLINLGVYKDVQAELAAAGKSLAQVEDVEPEPSLGNGGL
                     GRLASCFVDSMSTLAINGEGVGLNYHCGLFRQVFKKNEQEAEPNFWIENDSWLIPTTI
                     SYDVPFKNFTLKSKLDRLDILGYKKETKNYLNLFDIESVNYDLITDGISFDKTDIKEN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLLIDEAVERGSNLHDLADYAYVQINDT
                     HPSMVIPELIRLLTEKHGIEFAEAVAIVKNMTGYTNHTILAEALEKWPLEFLEEVVPH
                     LVDIIKELDALIRAEIKDPAVQIIDESGRVHMAHMDIHFSNSVNGVAALHTEILKNSE
                     LKAFYELYPEKFNNKTNGITFRRWLEFANQDLADYIKELIGDEYLTDATKLEKLLAFA
                     DDKEVHAKLAEIKFNNKLALKRYLKDNKGIELDENSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKNGNLPKRKITVIFGGKAAPAYIIAQDIIHLILCLSELINNDPEVSKYLNV
                     HLVENYNVTVAEKLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGMDNIYTFGKDSDTIIDLYDKAGYVSADYYNGDANIKRAVDFIVSDEVKALGNEE
                     RLGRLHHELISKDWFMTLIDLAEYIEVKEQVFADYEDQDSWNKKVVHNIAKAGFFSSD
                     RTIEQYNQDIWHSN"
     gene            complement(7417..8925)
                     /gene="malQ"
                     /locus_tag="ACHU66_000785"
     CDS             complement(7417..8925)
                     /gene="malQ"
                     /locus_tag="ACHU66_000785"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR3389084.1"
                     /translation="MTNRTSGILMHITSLPGKFGIGTFGQSAYDFVDFLVETKQTYWQ
                     ILPLTTTSYGDSPYQSFSAIAGNTHLIDFDLLVEDGLLATEDFQDVNFGDNPEKVDYA
                     LIYQVRRPILEKAVQAFLQDDKKKAAFLEFEKANSSWLTDYAEFMAIKEYFGNKALQE
                     WEDKKVVARNEEALDKYRLELANQIDYYKVTQYFFFSQWKQLKEYANKHHIKIIGDMP
                     IYVSADSVEVWTKPQLFKLDSERKPLYVAGVPADNFSADGQLWGNPIYDWPEHEKTGY
                     NWWIYRIHESFKLYDVLRIDHFKGFSDFWQVDGKAEVAKVGTWEPGPGYNLFKAVKET
                     LGDLPIIAEDLGNIDAKARKLLADCGYPGMKILEFGFFDVTGKSIDAPHRCIPNSVAY
                     TGTHDNEVVNGWYNNLEPEQQEYVDAYSNRKPIEKVSQAMLRMLFATVSDTAIATMQD
                     VLDLGEESRMNMPSTIGGNWEWRMKAEDLTQERKDFLTKMTLLYQRGNENHD"
     gene            complement(9107..11206)
                     /locus_tag="ACHU66_000786"
     CDS             complement(9107..11206)
                     /locus_tag="ACHU66_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR3389085.1"
                     /translation="MNNNFNNFNNMDDIFNQLMGNMGGYSTERRRYSINGREVTPEEF
                     AMYRQTGRLPQTEEVAQTQAKGQIKSDGILAKLGRNLTQDAREGKLDPVIGRNKEIQE
                     TAEILARRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGLEA
                     GTQYRGAFEENVQNLVDEVKKAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKPAL
                     SRGELTVIGATTQDEYRNTIHKNAALARRFNEVKVNAPSAEDTYQILKGIKPLYEAHH
                     NIELPDEVLRAAVDYSVQYIPQRSLPDKAIDLIDVTAAHLAAQHPVTDIQTLEAEMAE
                     AKQLQLEAAEKEDYEKALNEKVRIDKLQKQIDNHTEQQKVVATVNDVAQAVERMTGIP
                     VSQMGASDIERLKELKNRLAANVIGQDDAVEAVSRAIRRNRAGFDDGNRPIGSFLFVG
                     PTGVGKTELAKQLALDLFGNKDAIIRLDMSEYSDRTAVSKLIGTTAGYIGYDDNSHTL
                     TERVRRNPYSIVLLDEIEKADPQVITLLLQVLDDGHLTDGQGNQVNFKNTIIIATSNA
                     GFGYGMAEGEENQDIMDRIAPFFRPEFLNRFNAVIEFKHLDKDDLKAIVELLLAQVNN
                     TLAKKGIQLTVTEAAKGFLMEEGYDKAMGARPLRRVIENQIRDKVTDYYLDHVDVKYL
                     EADVVDGSIQIKEQSFA"
     gene            complement(11621..12031)
                     /locus_tag="ACHU66_000787"
     CDS             complement(11621..12031)
                     /locus_tag="ACHU66_000787"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR3389086.1"
                     /translation="MIQPIMKDIFFLQQKSEPATQLDVQVGQDLQDTLSANAHACVGM
                     AANMIGIKKRIIIVNMGFTNLVMYNPVLISKAKPYQTEEGCLSLEGTRPTTRYQEIEV
                     EFLDASWKKISLKLTDFQAQIVQHELDHLEGIII"
     gene            complement(12051..13430)
                     /locus_tag="ACHU66_000788"
     CDS             complement(12051..13430)
                     /locus_tag="ACHU66_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HFR3389087.1"
                     /translation="MKEKICKKLKELAKKPVRRIDWSGERWFVRRYKQGLALAWIVYF
                     LLREELVKEILKPVSNYLMNFSCIQWFYVNKLTVLLVFTALLFLAKSIFSILRWCEVI
                     RYFPLGKKKYPEYDFPFQEELVSFLNRKEERGRNIFWLDGSWGSGKTHFIRTFFENQL
                     YKPREIYYISCFGIRTREQAEKALIEEIEQHSTFGSLDHIPVVSSLVKWSYKILGLDL
                     MKKYSLIIFDDLERVAYSEKQKDKPEDFNDLLGFIDHLANHRNQKVLVLMNKEDMGNT
                     YQKIVEEKFKPVVTNIPSQKDIIKNMTEEYDDMSREFIRNIYLFQHEFGFINLRAIPF
                     MYSVFESVRQNGVYSVEWFVTTLNNQMREYMTSIMREVRGEVGEEVIYIDEYLNDNAY
                     WKGYLDELNKEDYLNMENKLDIYGEILKQLDKEFNSEKNLIIIKYRDSVYEFAIDNDT
                     LRLIESTYS"
     gene            complement(13490..13870)
                     /locus_tag="ACHU66_000789"
     CDS             complement(13490..13870)
                     /locus_tag="ACHU66_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HFR3389088.1"
                     /translation="MKKEMSQLEAYKAEAKERWGQTATYAEFEVRYDASKDHLFAQEM
                     QAIFEAFGKMQSLGAVHPDVQAQVANLQAYITENFYTCTKEILQNIGLMYVEDERFSA
                     NIDRAGGLGTAAFVSQAIAIYCQE"
     gene            complement(13872..14960)
                     /locus_tag="ACHU66_000790"
     CDS             complement(13872..14960)
                     /locus_tag="ACHU66_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFR3389089.1"
                     /translation="MISGIIAEYNPFHTGHKYLLEQAEGLKIVVMSGNFMQRGEPAIV
                     DKWTRAQMALEHGADLVVEMPFLVSVQSADHFAKGAISILHKLGVEKLVFGTEEMLDY
                     QKIADIYVDKSEEMENFVKNLPDNFSYPQKTQAMWQEFAGLTFTGDTPNHILALAYAK
                     AVAGTGIQLSPVQRQGAGFHSEEVETSYASATAIRKGADQLDLVRGFLPSVSLFEEAT
                     KVSWRNYFPLLRYQIATHPDLSQVFQVNEELASRIRSAIGSVATVEELVDAVATKRYT
                     KARVRRVLTYILINAVETPLPEAVHVLGFSTRGQAYLKQVKERVDLVTRIGKEPWDSL
                     TQQADAVYQLGADAMAEQTYGRVPVRVE"
     gene            complement(15004..16773)
                     /locus_tag="ACHU66_000791"
     CDS             complement(15004..16773)
                     /locus_tag="ACHU66_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389090.1"
                     /translation="MNVAIIILVAIFVILLLLLLVYVALFQIWLKSSRQIHEYDEDII
                     RDYLCKLAESFEYSSTQIPYGRARWFILGSKDFVCTVNDVENLEIFGYSPIRSKIDEE
                     FLEYGLLLTQDGFYFREQIENSNNDADVKYKISKKFFPLKGLWKITCKGKKILLFYPN
                     KIERLNFQFDDAQQKNIVANLNSIIKTGYTADLHENYLEKVIDRLQKNDFHTENFQNG
                     AKIGSIIGLNMMLGFHLKDIQFSAIAKAPQGHGHAAEYANNVIDKIKYPFQKVEQVGR
                     DNVKNGADRIVGNQKIQTKYYAKASGTINAAFDKANGGEYRYKGMQLEVPKDQYNESI
                     QLMKKKIKAGKVPGHSEPDDAYLIIRKGNVTYNEAKLVAKGGNIVSFSQQIGKILTKK
                     IADQTVKNVAANTVARQAAGVISFGITVGPDVFDLLQGRISSQQLLKNSLVAGTGLLG
                     GVAGGAVAGAFLGPVGSFVGAVAGGTLAAVGSKVVLDNYIEDDRIEMFAILKEEFIDI
                     VMAMALSEEEFKEIQNNIFDKKLGERLKDMFQKKQSVKNRMFTRESIIEREVERVISK
                     RTVLAEEDILEAVQIAEKEFAVI"
     gene            complement(16786..17532)
                     /locus_tag="ACHU66_000792"
     CDS             complement(16786..17532)
                     /locus_tag="ACHU66_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR3389091.1"
                     /translation="MATYETFAAVYDEIMDDSLYDKWTDFSLRNLPQDTKTILELACG
                     TGIQAVRFAQKGFEVTGLDLSYEMLELAQKKAEVAGVMMELAQADMMALEGVGDFDAV
                     TCYSDSLCYMADQEAVLRVFEGVHSVLNQGGTFLFDVHSIYQMEEVFAGYSYHENYED
                     FAFVWDTYEGEYAHSIVHELTFFVKDEDSQEERFIRRDEVHEERTYPIETYVELLKEA
                     GFASVEVFADFENQAPTETSQRWFFKCKKK"
     gene            complement(17989..18348)
                     /gene="rsfS"
                     /locus_tag="ACHU66_000793"
     CDS             complement(17989..18348)
                     /gene="rsfS"
                     /locus_tag="ACHU66_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR3389092.1"
                     /translation="MKELDLVKVVVQAADDKRAEDLVVIDVQGVTSLTDYFVIASSMN
                     SRQLEAIAENIREKVAEVGIKSSRVEGDSNGGWVLLDLGGLVVHIFSEEMRDLYNLEK
                     LWHEGSFLEVADFLEEE"
     gene            complement(18350..18859)
                     /locus_tag="ACHU66_000794"
     CDS             complement(18350..18859)
                     /locus_tag="ACHU66_000794"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFR3389093.1"
                     /translation="MKTALIVIDIQSMPVEEQPYAIEERLALWQDSLAQARQAGLEVI
                     HVRHHDQELVKGTADWEIHSIVAPLASEKIFDKTFNSAFKETRLHAYLQEKEIERLII
                     MGMATNFCIDTTIKVAFELGYKVAVIQDGTTTGYSGKLDAEDLIDHYQNIWSWNFAQV
                     DKLENIIRG"
     gene            complement(18869..19311)
                     /locus_tag="ACHU66_000795"
                     /pseudo
     CDS             complement(18869..19311)
                     /locus_tag="ACHU66_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735474.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(19314..19901)
                     /gene="yqeK"
                     /locus_tag="ACHU66_000796"
     CDS             complement(19314..19901)
                     /gene="yqeK"
                     /locus_tag="ACHU66_000796"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFR3389094.1"
                     /translation="MIAADLTFDRQALLEKIRVAMKPARFQHVLGVEEAALALADRYG
                     CDPKKASLAALLHDYAKEVEDQVFLDLIAKYDLDKALLNWDNNIWHGVVGAYKIAEDF
                     GLEDQEIFQAIQRHTIGAGQMTLLDKVLYVADYIEPNRDFPGVDEARRIAKESLDKAV
                     AYETAQTISYLAKKGIPIYPQTLETYNGYVVWLKE"
     gene            complement(19898..20530)
                     /locus_tag="ACHU66_000797"
     CDS             complement(19898..20530)
                     /locus_tag="ACHU66_000797"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR3389095.1"
                     /translation="MAIELLTPFTKVELEVEKKETNRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLKLDEVLLMPEFIPPHVDKKETIDEYHRYSMLKMAIAGIEGLGIETIELERR
                     GVSYTYDTMKLLKEKNPNTDYYFIIGADMVDYLPKWHRIDELVQLVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRSFIKQGRVPNFMVPHEVLDYIEEKGLYQ"
     gene            complement(20543..20854)
                     /gene="yhbY"
                     /locus_tag="ACHU66_000798"
     CDS             complement(20543..20854)
                     /gene="yhbY"
                     /locus_tag="ACHU66_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR3389096.1"
                     /translation="MMLTSKQRAFLNSQAHSLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKQVKE
                     I"
     gene            complement(21173..22279)
                     /gene="yqeH"
                     /locus_tag="ACHU66_000799"
     CDS             complement(21173..22279)
                     /gene="yqeH"
                     /locus_tag="ACHU66_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFR3389097.1"
                     /translation="MEELFCIGCGAQIQTLDKAVAGFTPQSALEKGLETGQLYCQRCF
                     RLRHYNEISDVNISDDDFLKLLHSVGESDALVVNVIDIFDFNGSVIPGLPRFISGNDV
                     LLVGNKQDILPKSVKTGKVTQWLTERAHEIGMRPVDVVLTSAQNKQAIKDLIEKIEQH
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDKDVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYISPRKEIKPKTYQLNPEQTLFLAGLGRFDFVAGERQ
                     GFTAFFDNELQLHRTKLQGASEFYQKHAGSLLVPPTSKELKEFPELVRHEFTINEKTD
                     VVFSGLGWIRVNEKAKIAAWAPKGVDVVIRKAII"
     gene            complement(22280..22807)
                     /locus_tag="ACHU66_000800"
     CDS             complement(22280..22807)
                     /locus_tag="ACHU66_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFR3389098.1"
                     /translation="MSLENYMPDFALEKAYDVTVESLKKHGIKVVFVDLDNTLIAWNN
                     PDGTPEMRQWLHDLQDAGIPVVVVSNNKYERVKRAVEKFGIEFEAFALKPFTFGINRA
                     LKRFDVQPYEVIMIGDQLMTDIRAAKRAGLKSVLVKPLIKTDSINTQINRWRERRTMK
                     KIIAKYGAIDYKREM"
     gene            complement(22991..23365)
                     /locus_tag="ACHU66_000801"
     CDS             complement(22991..23365)
                     /locus_tag="ACHU66_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389099.1"
                     /translation="MNPLRKELRTVAVEVSDLALDYAVRLAQSLNSTLRYHNYDSLIA
                     IAKTKGVEPKGKDCQSFSEYRQRYSLYDAKKLIYRALAWRLFDDSHADYGHALTILGL
                     DEDESGVEQIGFAFSKFTLDID"
     gene            complement(23572..24201)
                     /locus_tag="ACHU66_000802"
     CDS             complement(23572..24201)
                     /locus_tag="ACHU66_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR3389100.1"
                     /translation="MIKEFCSENHVAVVKALSLGAQRVELCDNLAVGGTTPSYGVIKH
                     VCQLAHENEATVMTMIRPRGGDFCYDEAEVEMMVEDCKAAIELGADGLVYGLLTEENW
                     LDEPALERLFAVSQDCQIVFHMAFDQIPRERQFEALDWLVEHGVTRILTRGSLTGSAL
                     NNVEWLQEIVAYAQGKIEILIGGGLTVDNVPTLLEALPINQVHGTRLFW"
BASE COUNT         6891 a         5591 c         4461 g         7287 t
ORIGIN      
        1 taggagaagc caaatgaaaa ggaacaagca tctcccctta acggagacaa cctactatat
       61 tctattagct ctcttggaac ctgcccatgg ctaccatatc atgcaaaaag tagaggatat
      121 gagtgatggt gatgtgaaaa tcgctgcagg aaccatgtat ggagcgattg agaatctttt
      181 gaaactaaaa tggatttgtt ccgttccaag ttccgataaa agaagaaaag tctatcaagt
      241 aacagcaacg ggccaagaga ttattaaact ggaaacacag cgtattcaaa aactacatca
      301 tattgccaaa gaattaggtt tttaaggagg aagccatgat aagacacaaa ttattcacca
      361 gtttacaaga ggaagaacat tggattaatt cgattcaatc cgaaggttat cagcttgtca
      421 aagtaactcc gtggacagca gcctatcatt ttgaaaaatg tagtcgaacc cctcatcccg
      481 tacgccttga ttttcatgaa catattgcaa aaggagaata ttcaaattac ctctctctat
      541 ttgaagactg tggttggcaa cctatacaag gtagtcgtag atgtggcatc cattattttc
      601 agcaaacaat cgaagcttca agcactgaaa ttttttctga taccgaatcg aaaaaagcat
      661 tctattcacg ctaccaaaat tatgcctact cctatttcgg attatttctg actttatttt
      721 ttatccatta ccaagttgga ctccaaaatg gatacacact ttggaatcct aaatcctggt
      781 acctaactcc agaactatgg gaacgaactg gtaccagttt ttggtttggt tttctctttg
      841 aaaccccatt cgcattgttt agaagtggca ttattccctt atttttcctt gcttggtcta
      901 tttatttcct ccatatcgct gaaaaaggta aaaaagaagc gaagaaatta gaaaaatact
      961 gatggcatac tatccaaaag aaaaagattg aaaagagttg actacaatca tcttttcaat
     1021 ctttttttac tttttacaac tcaactgtcg caacttgact atcttgacta acctgtccaa
     1081 gatttccaag gctgacagat ttgatttcag cagtatttgt gaaggcaaca acgatggttg
     1141 tttcacgtcc tgctgagcga atggcatcca gatctgcgac aaggagcaag tctcctgctt
     1201 caacgcgttg cccatctgtt accttagctg agaatggggc accgtttagt gcaaccgtat
     1261 ctaggccgac gtgcaccaag acttctacac ccttgtcaga caaaatcccg acagcgtgtt
     1321 tggttgggaa gacactggtt acgatacctg aaactggtgc gtaaacattg ccggagctag
     1381 gctcaactgc ataaccgtcg cccatcattt tttgtgagaa gaccggatct ttcacttctg
     1441 tgatggcaat ggcttgccct tcagcaacag agtaaactgt ctccgtcaca cctttgaact
     1501 gagtctcagg agcaacttct tctgtcgcaa caatttccgt acgagggatg gctacgcctg
     1561 attctagtaa gtcttgaatg tctgatttga gaacatctgc ttttggtccg tagactgctt
     1621 gaacacctga gtctttgata atcaagccca tagcaccagc ctgtttccaa gcattttcat
     1681 ctccaacctg tgccacatct ttaacactaa cgcgaagacg agtcatacaa gcatccacat
     1741 ctgcgatatt atcacgacca ccaagcaagt tgatgatttg aaccacttgt gaattggcat
     1801 ccgcagtatc tacgtttgag tttgaaacca catccgttgt ttctgtatca tagttaccat
     1861 tgcgtccagc tgttgctagg ttgaatttct taatcataaa gttggcaatg aagtacatac
     1921 caaccgcaaa gagggctgtt acccagataa agttaataac atccataccg ataccagctt
     1981 tgatcgccat cggtgtacgt gtaaggaatt cgatattacc aaatgagtgc atacgcaagt
     2041 tgacaacgtc agccatggca aaagccgcac cctgtacaaa agcataaacg acatagagtg
     2101 gcattgctgc aaacatgaac ataaactcaa ttggttctgt tacacctgtc aagaatacgg
     2161 caagtgctga agaaataaac atacctttgt acttatgttt tttatcttca tccacattgc
     2221 ggtacatagc gtaagtcaat cccatcaaaa tccctgatga accaatcatt tgaccaactt
     2281 tgaaacgggc tggagtaatg tttgcaagaa ggtcattgta ctgagccata tcaccagcgt
     2341 ctttgaggtt gatcaagtct gtaatccaag ccaaccagag cggatcttgt ccaaatactt
     2401 gtgcacctgc ttgtgcacct gtcaagatgt cataggtacc accaagtgat gtatagttca
     2461 ttggaatggt caacatgtgg tgcaaaccaa atggaagaag caaacgttcc aaggtaccat
     2521 aaacaaaagg tgcaagaatt ggtgctgtat cctgtgagct tgcaatccac ttaccaaagc
     2581 tgttgattcc tgtttgaaca actggccaaa agatagctaa tcctagcgct acaagaactg
     2641 agcgatagat aaccacaaat ggtacgaagc gtttaccgtt aaagaaggtc aaaacatcag
     2701 gtaatttgcg gtaattgtag tatttgttat aggttgttgc ccctacaaaa ccagcgatga
     2761 taccaacgaa gacacccatg ttcagagcag gctgacccaa gatgttgacg aagtagcctg
     2821 aaaccggaat ctctgttcca aaaacagtgg ttacggttgc cgctgcatct gcaagcatgt
     2881 cagtcgtcac tccaaagaag tgacctgtaa tcaagttaat aaggatgaag gcaagacctg
     2941 ctgagaaggc accaccagcc ttttccttag cccagctacc accgatagcc aaggcaaaga
     3001 gcaagtgaag gtttccgata atcccccaac cgatttgggc gataatattc ccgatacgaa
     3061 tcaagagttc tgattctggg ctgattaacg gaatagagtt tccgatagaa accatcagac
     3121 ctgctgcagg cataactgca atcacgacca ttaaacactt accgaatttt tgccaaaatt
     3181 cgaaactaag aaattttttc attttttctc ctttattttt tggctctgtg ccattttata
     3241 cattaatttc aacttccaca ccaaaatggt ctgaaataat cggacgattc tctccattga
     3301 aaatcactaa gctggataaa acttcctttt tctgatttaa gaaaatgtaa tccaatcgtt
     3361 tttcctgact gtgaccagcc cagccatcga tagccttttc aaccgtaatc ccctggtctt
     3421 tttccatcgc tagattgtat gaatcataaa gcccgagctc tgtaatcgct tcgtaggcct
     3481 cgcgatctga gaaggcatcg gtattaaaat ctcccatgag aatcttaaaa cgtctattgc
     3541 cagtccgttc cacaatcgta cgaacgttat caagctggtc ttcctcttca ctccctggaa
     3601 gattgatgtg acaagaatac aggtcgatca actggtcttg atagaagacc gtcaagccca
     3661 taatttttcg tgataggatg gaatcaacag atttattctg actacaataa aatggatcca
     3721 cttcataaac tggcaacttg gtaagaaagg caatcccttc ttcgtactta tcgtagccga
     3781 tatgtgagtt gctccaataa tatgaataat caaattgtcc caaggctctt aatttttcta
     3841 ataaaatcaa gccataatta tccgccttca agtctttcgt taccaaatca gcagttatga
     3901 gctgattgac ctcttgaaga gcgatgacat cataagcctt ttcagctatt gttttggcta
     3961 aaacatctag tttttcatcc tgcctttctt ctaaccaggc atggacatta attgttagta
     4021 atttcattgc tccccctctc tctgaaaatg cgtaaccgtt ttcttttata gaaatagtat
     4081 aacgtaaacg tttgcattag tcaatagctt ttccgtaata tttcaaactt ttacgtaaaa
     4141 gttgtgtatt ctactaaata aaatagatgt attgcccctt tctggctctt ttatgataca
     4201 ctaatgggta gaaagacagg aaactatgag taagtataaa aaagtttatc aagatattaa
     4261 gaaaaaaatc gaagagcagg tttggtcaac tggacaggcc ctaccgacag aaaatgaatt
     4321 aatggaagtg tattcctact ccaaggacac cattcgcaaa gcactatctc ttcttgaaat
     4381 ggatggttat attcaaaaga aacaggggaa atcttctatt gttatcgaac atggtctaat
     4441 gaaggaccaa tatctttctg aaattaaaac agcgggcgaa ttgaataaaa gatcacagca
     4501 ccaaattcag acccagctta cttcgcttta tattattcaa ggccaagaag atttaatgtc
     4561 aactttcgaa gttgatgata aggtagattt ctatcgagtg agtcgggtac ggacaattga
     4621 cggtgagcgg ttggaatatg acatttccta tttcgaccgt agggtcgttc catatctcag
     4681 taaagaaatt gccgaaagct ctatttaccg ctacttagaa gaagaactcc atttaacaat
     4741 ttctcattcc agacgtgaaa tctccttccg ttttgcaaac gaagaagaaa aacaattgat
     4801 ggacttagga gactatgaca tggtcgtcgt ggtcactagt atcacctatc tttcaaatgg
     4861 tcaagctttc cagtatggaa caattagtta ccgtccagac aaagttagct ttgtatcaat
     4921 ggcgaaacgg taaaaggaaa ctttcgattt ttttgaccaa gtgtacaaaa cttctagaat
     4981 tttatagtta taaaaagaat gtctccgctt ttgcagagac attctttgca tttatatact
     5041 aaggagagca ccaataatgg taaaaactgg tagtttttat tcttcaacac ataggaagaa
     5101 ttgatctgat aagttttgga gtggttcaat caactttcct actaattgtt ggaattcaat
     5161 tagttactgt gccagatgtc ttgattgtac tgttcaatgg tacggtcaga tgagaagaat
     5221 ccagctttgg caatgttatg aacaactttc ttgttccatg aatcttgatc ttcgtaatct
     5281 gcaaagactt gttcttttac ttcaatgtat tcagccaagt caatcaaggt catgaaccag
     5341 tctttcgaaa tcaattcatg gtgcagacga ccaagacgtt cttcgttacc aagtgctttt
     5401 acctcatcgc taacgataaa gtcaactgca cgtttgatat tggcatcgcc attgtagtag
     5461 tccgcagata catatccagc cttatcgtac aagtcgatga tggtatctga atctttacca
     5521 aatgtataga tgttgtccat accagccaac tcagcaatct caacgttggc accatccatg
     5581 gtaccaagtg tcaacgcacc gttgagcatg aatttcatgt taccagtacc tgacgcttct
     5641 ttagaagcca atgagatttg ttctgaaata tctgttgcag ggatgagttt ctcagcaact
     5701 gttacgttat agttttccac caaatgaacg ttgaggtact tgctcacttc tgggtcattg
     5761 ttaatcaact ctgacaagca aagaatcaag tgaatgatgt cttgtgcaat gatgtaggca
     5821 ggtgccgctt taccaccgaa gataacagtg attttacgtt tcggaaggtt accattcttg
     5881 atttcaaggt atttatggat aacatacaag gcattcattt gttggcgttt gtactcgtgg
     5941 aaacgtttga tttgtgtatc gatgatagag ttttcatcca gctcgatacc tttattgtct
     6001 ttaaggtaac gtttgagagc caacttattg ttgaacttga tttctgccaa cttagcatgc
     6061 acttccttgt catcagcaaa ggcaagcaat ttttcaagtt tagtcgcatc tgtcaagtat
     6121 tcatcgccaa tcaattcttt gatgtagtca gcgaggtctt ggttggcaaa ttccaaccaa
     6181 cggcggaaag tgataccgtt tgttttattg ttgaattttt ctgggtaaag ctcgtagaaa
     6241 gctttcaatt cagagttttt gaggatttct gtgtgaagcg cagctacacc gttcactgag
     6301 ttagagaagt ggatatccat gtgagccatg tgtacacgac ctgattcatc aatgatttgg
     6361 actgctgggt ctttaatttc tgcacgaata agggcatcca attctttgat gatatcaacc
     6421 aagtgtggca ccacttcttc aaggaactca agtggccatt tttcaagtgc ttccgccaag
     6481 atagtgtggt ttgtgtaacc agtcatattc ttaacgatgg caactgcttc cgcaaattca
     6541 ataccatgct tttcagtcaa aagacggatc aactcaggaa tcaccattga tgggtgagta
     6601 tcgttgattt gcacataagc gtagtcagcc aagtcatgca agtttgaacc gcgctcaact
     6661 gcttcatcaa tcaaaagttg agcagcattt gataccatga aatattgttg gtagatacgg
     6721 agcaattcac cattcttatc agaatcatct gggtacaaga agagagtcaa gttttcttta
     6781 atatcagtct tgtcaaatga aataccgtct gtaatcaagt catagtttac tgactcaata
     6841 tcaaacaaat tgaggtagtt cttagtctct tttttatagc caagaatatc aagacgatcc
     6901 aattttgatt tcaaagtgaa gtttttgaat ggaacatcat agctgatagt tgttggaatc
     6961 aaccaagaat cattttcaat ccagaagttt ggctctgctt cttgctcatt tttcttaaat
     7021 acttggcgga aaagaccaca gtggtagttc agaccaacac cttcaccgtt gatagcaagt
     7081 gttgacatag aatcaacgaa acatgaagcc agacgtccca aaccaccgtt accaagtgat
     7141 ggttctggtt caacatcttc aacttgcgcc aatgatttac cagctgctgc caattctgct
     7201 tgaacgtctt tgtacacgcc caagttaatc aagttgtttg acaagagttt accgataagg
     7261 aactcagctg agatatagta aactttacgc tttcctgtgt tttttggttt tgaagctgct
     7321 gcatctttta cgtagttcaa cagttgaaga tagatttctt catttgtcaa atcagctaat
     7381 ttcttcgaag tatttgtttc tgcaaatgtt gtaaatttag tcatggtttt catttcctcg
     7441 ttggtatagt aatgtcattt ttgtcaagaa gtctttgcgt tcttgggtca agtcttcagc
     7501 tttcatgcgc cattcccagt ttccaccgat agttgacggc atattcattc gactttcttc
     7561 acccaagtct aggacgtctt gcatggttgc aatagctgta tcactcactg ttgcaaacag
     7621 catacggagc atagcttggc taactttttc aatcggctta cggttgctgt aggcgtccac
     7681 gtattcttgt tgttctggct caagattgtt gtaccagcca ttgacaacct cattatcgtg
     7741 agttccagtg taggcaactg aatttggaat gcaacggtgt ggagcatcga tactcttacc
     7801 tgttacatca aagaaaccaa attctaagat tttcatgcct ggatagccac aatccgcaag
     7861 caatttgcga gctttagcgt caatgtttcc aaggtcttct gcaatgattg gaaggtctcc
     7921 aagtgtttct ttgacagcct tgaagagatt gtagccagga cctggttccc aagtacctac
     7981 ttttgccacc tcagccttac catcgacttg ccagaagtct gagaaacctt tgaagtggtc
     8041 aatccgaagg acatcataaa gtttgaaact ttcatggata cgataaatcc accagttata
     8101 gcctgttttt tcatgttctg gccaatcgta aataggattt ccccataatt gaccatctgc
     8161 cgagaagtta tcagctggta caccagctac ataaagcggc ttgcgttcac tgtccaattt
     8221 gaatagttgg ggttttgtcc acacttcaac gctatcagca gaaacataga ttggcatgtc
     8281 tccgatgatt ttgatgtggt gtttgttggc atattctttc aattgtttcc attggctgaa
     8341 gaagaaatac tgtgtgactt tgtagtagtc gatttgattt gccaattcta agcggtattt
     8401 atcaagagct tcctcgttgc gagctacaac ttttttatct tcccattctt ggagagcttt
     8461 attaccaaag tattccttga tagccatgaa ctccgcataa tctgtcaacc aagatgagtt
     8521 agccttttca aactctagaa aagcagcttt cttcttatca tcttgtaaaa atgcttgaac
     8581 tgccttctcc aaaattggtc ggcgaacttg atagataaga gcatagtcta ctttttcagg
     8641 attatctcca aaatttacat cttggaaatc ttctgttgcc aataagccat cttcaaccaa
     8701 gaggtcaaag tctatcaagt gtgtatttcc tgcaatagca gagaaagact ggtaaggaga
     8761 atctccatag cttgtagttg ttagtggtaa aatctgccag taggtttgct tggtttccac
     8821 caagaaatct acaaaatcat aggcagactg tccaaaagta ccaataccaa acttacctgg
     8881 aagtgaggta atatgcatca aaattccact ggtacgattt gtcataaaaa cgttttcctt
     8941 tctgtcggtt acgaatatag tttaacgcaa acgtttgcac ttgtcaagga ttttataaaa
     9001 aaagttttaa tgtactctag aaagcctatt ttatagcatt tgttagcgaa taacaaaaaa
     9061 gaaaaatcag atatttctat ccgatttttc aaattcttca ctatttttaa gcaaagcttt
     9121 gctcttttat ttggatgctt ccatcaacca catccgcttc cagatatttg acatcaacat
     9181 gatccaaata gtaatctgtt accttgtctc gaatctgatt ttcaatcaca cgacgcagag
     9241 gacgggctcc catggccttg tcatagcctt cttccatcaa gaatcctttg gcggcttccg
     9301 ttaccgttaa ttggatgcct ttcttcgcca gggtattatt gacctgggcc aagagtaatt
     9361 ctacaatggc tttgagatca tctttatcca agtgcttgaa ttcgataact gcgttaaaac
     9421 gattaaggaa ctccggacgg aagaatggtg cgatacggtc catgatgtct tgattttctt
     9481 caccttctgc cattccataa ccaaaacctg cattagaagt cgcgataata atggtatttt
     9541 taaagttaac ttggttgccc tgtccatcag tcaaatgccc gtcatctagc acttgcaaga
     9601 gaagggtaat cacttgtgga tcagcttttt caatctcatc cagaagaacg attgagtacg
     9661 gattgcggcg gacacgctcg gtaagagtat gggaattgtc atcgtagcca atgtagccag
     9721 ctgtggtacc gattaatttg gaaacggctg tccgatcgct gtattcagac atatccaaac
     9781 gaataatggc atccttgtta ccaaacaagt ctagtgctag ttgcttagcc agctctgtct
     9841 tacccacacc tgtcggaccg acaaagagga aggaaccaat cggacggttg ccatcatcaa
     9901 aacctgcacg attccgacga atggcacgag atacagcttc caccgcatcg tcttgaccga
     9961 taacgttagc tgccagacga tttttcaatt ccttgagtcg ttcaatgtca gaagctccca
    10021 tttgagagac tggaattcct gtcatccgct caaccgcctg agccacatca ttaacagtcg
    10081 ccaccacttt ctgttgctcc gtatgattat caatctgttt ttgtagctta tcaatacgga
    10141 ctttttcatt caaggctttt tcataatcct ctttctcagc cgcttccaac tgcaactgtt
    10201 ttgcctcagc catttctgct tccaaggtct gaatatctgt cacaggatgt tgtgcagcca
    10261 agtgtgcagc ggtcacatcg atcaggtcaa tagccttatc aggcaagcta cgttgtggaa
    10321 tatattggac agaataatca accgcagctc tcaaaacctc atctggcaat tcaatattgt
    10381 ggtgggcttc ataaagcggc ttaatccctt tcaaaatctg gtaggtgtct tctgcggacg
    10441 gagcattaac cttgacttca ttgaaacgac gagccagtgc ggcgttctta tgaatggtat
    10501 tgcggtattc gtcctgtgta gtggcaccga taaccgtcaa ttccccacgg gataaagctg
    10561 gtttgagaat atccgctaaa cccttggaac ctgagtctcc gccagtgcta ccagctccca
    10621 aaatctgatg aatttcatca aagaagagaa tgatatttcc agcttttttg acttcatcca
    10681 caagattttg cacattttcc tcaaaagctc cacggtactg ggtccctgcc tccaagccag
    10741 aaatgtcaat ggagatgatt tccttgttct taatggctgc tggcacatct ccgttgacaa
    10801 tggcttgagc caatccttct actaccgcag ttttaccaac accagcatca ccgaccaaaa
    10861 caggattatt tttcgtacgg cgagccaaaa tttccgctgt ttcttggatt tccttgtttc
    10921 gtccaatgac tggatccaac ttaccttcac gcgcatcttg ggtcaaattg cgccccagtt
    10981 ttgcaagaat cccatctgac tttatctgac ctttagcctg tgtttgagcc acttcttcgg
    11041 tctgtggcag gcgaccagtc tgacggtaca tagcgaattc ctccggtgtc acttcacgcc
    11101 cattgataga atagcgacgt cgttctgtac tgtatccacc catgttcccc ataagttgat
    11161 tgaaaatatc gtccatattg ttaaaattat tgaaattgtt gttcatagta gttacctcgt
    11221 ttaagtttca gttatttttg acttttactg acctttgtca gaaaataaaa tctaataggg
    11281 tataatcctg tctttcatag caacctcctc agaagtttgt cttttactga ccaataggtt
    11341 caagagtaag tcaaaccgtt ctcgtttgac ctaaactgac cttttgttca aagaatttgt
    11401 aaggcccttt accttacgaa tcttatttta catcattggt cagtaaaagt caatgatttt
    11461 gtttgacctt ttttgacttt ttatagtcga atgaattagc tttcagacaa ggaactgagg
    11521 cgcaggctat actaaagtat ggcaaggcaa aagtgacgat gtatcaaagt taattcaaat
    11581 gactataaaa aaatctacct gacggtagat ttggtagcta ctagataata atcccttcca
    11641 gatgatctag ttcatgctga acaatctggg cttggaagtc tgtcagcttt aggctgatct
    11701 ttttccaaga agcatccaaa aactccactt caatctcctg atagcgtgtg gtcgggcgag
    11761 taccttccag cgacaggcag ccctcttctg tctgataggg cttggccttg ctgatgagga
    11821 cgggattgta catgactagg ttggtaaaac ccatattgac gatgataatc cgctttttga
    11881 tgccaatcat gttggctgcc atgcccacgc aggcatgagc gttggctgac aaagtatcct
    11941 gcaaatcctg accgacctgc acatctagct gagtcgcagg ctcagacttc tgctgcaaga
    12001 agaaaatatc tttcataata ggttgaatca tggggtagct ccttgctttg ttatgaatat
    12061 gtactttcta ttaatcggag agtatcgtta tcaatagcga attcatagac agaatctcta
    12121 tattttataa taataagatt tttctcacta ttaaattctt tatccaactg tttaagaatc
    12181 tccccataaa tatctaattt attttccata ttaagataat cctctttgtt taattcgtcc
    12241 aaatacccct tccagtatgc attatcgttc aaatattcat caatataaat aacttcttct
    12301 cctacttctc ctctcacctc tctcatgata ctagtcatat attctctcat ttgattattt
    12361 agtgttgtaa caaaccactc tactgagtac actccgtttt gccgaacact ttcaaataca
    12421 ctatacataa aaggaatggc tcttaaattt ataaaaccaa attcatgttg aaagagatag
    12481 atatttctta taaattcccg actcatatcg tcatactcct ctgtcatatt ttttatgatg
    12541 tctttctgac ttggaatatt tgtcacaacc ggcttaaatt tctcttcaac aatcttttga
    12601 taagtattcc ccatatcctc cttattcatc aagacaagta ctttttgatt acggtggttg
    12661 gcaaggtggt ctatgaaacc aagaagatcg ttaaagtctt caggtttatc tttttgcttt
    12721 tcactataag ctacacgttc caaatcatca aagataataa gagaatattt tttcatcaag
    12781 tctaagccca aaatcttata agaccatttg actaaactac taacaacggg aatatggtct
    12841 aaacttccaa aggtcgaatg ttgctcaatc tcttcaatca atgccttctc cgcctgttcc
    12901 ctggtccgaa tgccaaaaca ggagatataa taaatctcac gcggtttgta aagttgattt
    12961 tcaaagaatg tgcgaataaa gtgggttttc ccactccccc acgaaccatc taaccaaaag
    13021 atattcctac ctcgttcttc ctttcgattc aagaaagaaa ccagttcctc ttgaaaaggg
    13081 aagtcatatt cagggtattt tttcttaccc agtggaaaat agcggataac ctcgcaccat
    13141 cgcaagatgg aaaagatact cttcgcaagg aaaagcagag cggtaaatac taacaaaaca
    13201 gtcagcttat ttacataaaa ccactgtata caggaaaaat tcattaaata gttactaaca
    13261 ggtttaagaa tttccttcac caactcttct cgcaaaagaa aatagacaat ccacgccaaa
    13321 gctaaacctt gcttgtatct tcggacaaac catctctctc cgctccaatc tattcgtcgt
    13381 acaggctttt tggcgagttc ttttaacttc ttacaaattt tttctttcat aacttcctcc
    13441 tcagtttagt ataccaaaaa aagcctagtt ctcaccagac ttctttcgtt tattcttggc
    13501 aataaatcgc aatagcttga ctgacaaagg ctgctgttcc tagtccacct gctcggtcaa
    13561 tgttggctga aaatcgctcg tcctcaacat acataagacc tatattttgc aagatttcct
    13621 ttgtgcaggt gtagaaattt tccgtgatat aggcctgcaa gttcgctacc tgagcctgca
    13681 catctggatg aacggctccc aagctctgca tttttccaaa agcttcaaaa atagcctgca
    13741 tctcttgggc aaacaagtgg tccttgctag catcatagcg tacttcaaac tcggcatagg
    13801 tcgcggtctg tccccagcgt tcctttgctt ccgccttgta ggcttctagt tgactcatct
    13861 ctttcttcat ctcactccac cctcaccggc acgcgcccat aggtctgctc tgccattgcg
    13921 tcagcgccca gttgatagac cgcatccgcc tgctgggtca ggctgtccca gggctctttg
    13981 ccgatccgcg tcaccaggtc cacccgctcc ttgacctgct tgagataggc ctggcctcga
    14041 gttgaaaagc ctaggacatg gaccgcttct ggcaagggag tttccaccgc attgatcagg
    14101 atgtaggtca gcacccgccg cacccgcgcc ttggtgtagc gcttggtcgc aacggcatcc
    14161 actaactcct ccacggtcgc tacgctccca atggcagagc ggatacgact ggctagttct
    14221 tcgttgacct gaaagacctg actgagatct gggtgggtcg caatctggta gcggagcagc
    14281 ggaaaatagt ttctccagct taccttggtc gcctcttcga aaagactgac agacggtaaa
    14341 aagccgcgaa ccaagtccag ctggtcagca cccttgcgga tagctgtcgc cgaggcgtag
    14401 gacgtttcca cctcttccga atgaaaacca gctccctgcc gttgaacagg gctgagctga
    14461 atgcctgttc cagccactgc cttagcgtaa gctagagcca gaatatggtt gggcgtgtca
    14521 cctgtgaaag tcagcccagc aaactcctgc cacatagcct gggtcttctg tggataagag
    14581 aaattatctg gcaagttttt cacaaaattt tccatctctt ccgacttatc cacatagata
    14641 tccgcaattt tctggtaatc cagcatttct tccgtaccga aaaccagttt ttccacacct
    14701 agcttgtgta aaatgctgat tgctcctttg gcaaaatggt cagccgactg gaccgacacc
    14761 aaaaagggca tctcgacaac aagatctgcc ccgtgttcca aagccatctg agcccgtgtc
    14821 cacttatcca caatggctgg ctcaccccgt tgcatgaagt tgcctgacat aaccacaatt
    14881 ttcaacccct cggcctgttc cagcaggtat ttatgccccg tgtgaaaggg attgtattct
    14941 gctattatcc ctgaaatcat gccctacctc ctagatttta ttcaaccatc tacaacctaa
    15001 tacctaaatt acagcaaact ctttttctgc aatctgtact gcttcaagta tatcctcttc
    15061 tgctaaaacc gtgcgttttg agataactct ttcaacctct cgttcaatta ttgactctct
    15121 agtaaacatc ctatttttaa cagattgctt tttctggaac atgtctttaa gtcgttctcc
    15181 taatttctta tcgaagatat tattttgaat ttctttaaac tcttcttctg aaagcgccat
    15241 agccataaca atatcaataa attcttcttt tagaattgca aacatctcaa ttctgtcatc
    15301 ttctatatag ttgtctaaaa caaccttaga accaacagca gccaatgttc caccagctac
    15361 tgcacctaca aatgatccaa ctggacctaa aaatgctcca gcaaccgcac cacctgcaac
    15421 tccacctagt aagcctgttc ctgctactag gctatttttt aataattgtt ggctagatat
    15481 tctaccttgc agtagatcaa atacgtccgg acctacagta attccgaatg aaataactcc
    15541 tgctgcttgt ctagcaacag tattggctgc gacattctta actgtttgat cagcgatttt
    15601 tttagtaaga atttttccaa tttgttgaga aaatgaaaca atatttccgc ccttggcaac
    15661 cagttttgcc tcattataag taacattccc ttttctaata atcagatagg catcatcagg
    15721 ctcactatga cccggaactt ttccagcttt tatttttttc ttcatgagtt gaattgattc
    15781 attatactgg tctttaggaa cttctaactg catcccctta taacggtatt ctcctccatt
    15841 agccttatca aatgctgcat ttatcgttcc actagctttt gcatagtatt ttgtttgaat
    15901 tttttgattt ccaacaattc tatctgcccc atttttaaca ttatctctcc ctacttgctc
    15961 tactttttgg aaaggatact ttatcttgtc tattacatta ttagcatatt cagctgcatg
    16021 cccatgtcct tgaggagctt ttgcaatagc actaaattga atatctttca aatgaaaacc
    16081 tagcatcata tttaaaccta taattgatcc tattttagct ccattttgaa aattctctgt
    16141 atgaaaatca tttttttgca aacgatcaat aactttttct aaatagtttt catgtaaatc
    16201 tgccgtataa cctgttttga taattgagtt taaatttgca actatatttt tttgttgagc
    16261 gtcatcaaat tgaaaattta gtctctcaat cttattagga taaaaaagta gaattttttt
    16321 acccttacaa gtaatcttcc ataaaccttt taaagggaaa aactttttgc taattttata
    16381 cttaacatca gcatcattat tgctattttc gatttgttct ctaaaataaa atccatcctg
    16441 ggttaacaac aaaccatatt ccaaaaactc ctcgtcaatt ttgctcctaa taggactata
    16501 gccaaaaatc tccaaattct ctacgtcatt aactgtacaa acaaaatctt tgctccccaa
    16561 tataaaccat ctagcccgac cgtagggaat ctgcgtacta gaatattcaa aagactcggc
    16621 taatttgcat aaataatcac gaataatatc ttcatcgtat tcatgtattt gacgacttga
    16681 ttttaaccat atttgaaaca aagctacata aactaataat aacaataaaa gaataacaaa
    16741 tattgcaacc aagataatga tagctacatt catatccaat accacctatt tcttcttaca
    16801 cttgaaaaac caccgctggc tagtttctgt cggagcttga ttttcaaagt ccgcaaagac
    16861 ttccacagag gcaaagccag cttctttcaa taattccaca taggtttcaa tcggataggt
    16921 ccgctcttca tgaacttcat cccgacgaat aaaccgctcc tcttgactgt cttcatcttt
    16981 gacaaagaag gtcaattcgt gaacaatcga atgtgcatac tccccctcat aggtgtccca
    17041 gacaaaggca aaatcctcgt agttttcatg gtagctatag cctgcaaaga cttcctccat
    17101 ttgatagatg gagtgaacgt caaaaaggaa agtaccacct tgattgagaa cagagtgaac
    17161 tccttcaaag acccgcaaaa ctgcttcttg gtccgccatg tagcaaaggc tgtccgagta
    17221 acaggtcacc gcatcgaaat ctccaacacc ttccagagcc atcatatctg cctgggccaa
    17281 ttccatcatg acaccagcaa cttctgcttt tttctgagcc aattccaaca tctcatagga
    17341 taggtcaagc cctgtcactt caaagccctt ttgagcaaaa cgcacagcct gaatccctgt
    17401 cccacaggcc aattctaaaa ttgtcttggt gtcttgcggc aagttccgca agctaaaatc
    17461 tgtccattta tcatacaagc tgtcatccat gatttcatca tagaccgccg caaaggtttc
    17521 gtaagttgcc atatttccca ttttcccttt cataaaaagc agccaatcga ctgcttcatt
    17581 aactcacact gtgttcaaga agttcatcac ttctaattac ataatagtga actctctttg
    17641 gtcactttcg aattttatgg tgaccagctg aaacagttcc ccaaactgtt tcactcccac
    17701 ccccgcacag ctccaacagt cagagtagtg actgttggag gttggaaatg aagcgaactc
    17761 gttcgcatca gccgtatagg tcagatttgg agtgggaagc acgaacaaag ctgccaatca
    17821 accactgcgc tgagatgttg actcaagttt cgaatagtgc acccaaatca aaatctatct
    17881 tcatattttt caagaagttc atcacttcta atcacataat agtgaactct ctttggtcac
    17941 tttcgagttt tatggtgacc agctgaaaca attccccaaa ctgtttcact attcctcttc
    18001 taaaaagtct gcgacttcca ggaagctgcc ttcatgccat aatttttcaa ggttgtagag
    18061 gtcacgcatt tcttctgaga agatatgaac gactaggcct ccaaggtcca aaaggaccca
    18121 accaccgtta ctatcgcctt ctacacggct actcttgatt ccaacttccg ctaccttttc
    18181 acggatgttt tccgcaatag cctccaactg gcgactgttc atggaactgg caatcacaaa
    18241 gtagtctgtc aggctggtca cgccttgaac atcaattaca acaaggtcct cagctcgctt
    18301 gtcatcagca gcctgaacca caacttttac taaatctagt tctttcatct tatcctctaa
    18361 ttatattttc taatttatcc acctgggcaa aattccatga ccagatgttt tggtagtggt
    18421 caatcaagtc ttcggcatcc aatttgcctg aataacctgt cgtcgttccg tcctggatca
    18481 cagctacctt gtagcctagt tcaaaggcta ctttaatcgt cgtatcaatg caaaaattgg
    18541 tcgccatgcc catgatgatg agtcgctcaa tttccttttc ctgcaaataa gcatgaagcc
    18601 tcgtttcctt gaaggcactg ttaaaggtct tatcgaaaat cttctcacta gctaagggtg
    18661 caacaattga gtgaatctcc cagtcagcag tccccttgac caattcctga tcatgatgtc
    18721 ggacatggat gacttctagc cccgcttgac gggcttgagc aagactatct tgccaaagag
    18781 ccagtcgttc ttcaattgca tagggttgct cctccacagg catactctga atgtcaatga
    18841 ctatcaaggc tgttttcata tccactccct aaggcgtttc caaacggaag accaattccc
    18901 tttcttcttc actatccaag acaaaatcat ttttctccaa gagatgagcc atcttctgat
    18961 tgcccttaac ataagctgca atcaaatggt cgcagacagg atgtgattga gccattgcta
    19021 ttacaacctc caaagcagct tgaccatagc cccttccttg aaactcttgg caatcataaa
    19081 ccgccagagc atatactgct ggatgtcctc atcaatgtca atcaaggcaa aaccgaccac
    19141 ctgttgaccc gcccaaacag cgtaagggaa gacatccccg ttttctctgt aaagccaagc
    19201 gtcagcgagt gaacgcacat ttggagccac gaagcccttg tcctcctcgg ctactgtcaa
    19261 gtccaagact gcttgatagg agctttcatc cacaggtttt aatgcaatca tccctactcc
    19321 ttcaaccaaa caacatagcc attataggtt tccaaggttt gtggataaat gggaattccc
    19381 ttcttggcta agtaagaaat ggtttgggca gtttcatagg ctactgcctt gtccaaggac
    19441 tcttttgcga tacgacgcgc ctcgtccacc ccaggaaaat cccgattggg ttcgatatag
    19501 tccgccacat agagaacctt gtccagcaag gtcatctgcc cagcaccaat ggtgtgtcgt
    19561 tgaatggctt ggaagatttc ttggtcttcc aaaccaaaat cttctgcgat tttataggcc
    19621 ccaaccacac cgtgccagat gttattatcc cagttgagca gggccttgtc caaatcatac
    19681 ttggcaatca agtccaaaaa aacttggtct tccacttcct tggcatagtc atgcaagagt
    19741 gctgccagac tggctttttt cggatcacag ccgtatcgat ctgccaaggc aagcgccgcc
    19801 tcttccacac ccaagacatg ttggaagcgc gctggcttca tggctacacg gattttttcc
    19861 aaaagagctt gacggtcaaa ggtcaaatct gctgcaatca ttggtaaagt cctttttctt
    19921 cgatatagtc cagaacttca tgaggcacca taaaattcgg cacccgtcct tgcttgataa
    19981 agctccgtac catgctggag gaaatgtcca tcaagggcac atctacccag ataaccggat
    20041 aggacgtccc tgccttgtaa cgaggacgct ggacaccaac aaactgcacc aactggacca
    20101 attcatcaat cctgtgccat tttggaaggt agtctaccat atctgcaccg atgatgaaat
    20161 aataatccgt attaggattt ttttccttca gcaatttcat tgtgtcgtag gtatagctga
    20221 caccacggcg ttctagttca atggtttcaa tccccaagcc ctcaatacct gcaatagcca
    20281 tcttgagcat ggaatagcga tggtattcgt caatggtttc ttttttatcc acatgaggcg
    20341 gaatgaattc tggcatgagc aagacttcat ccagcttcaa ctgttgacgg acctggtctg
    20401 ccacaatcaa atgagcatta tggacagggt tgaaattccc tcccagaatc cccacttgtt
    20461 tgcgattggt ttctttcttt tcaacctcta attcaacctt ggtaaagggt gttaagagtt
    20521 caatagccat aggctctcct tcttagattt ctttgacctg tttagaaatt ttacggttct
    20581 ctttcttgct agattgtttg aataaaatca agatgcgacc gattttttgg accgtatcca
    20641 caccgatttc ttcttccaag atttctgcta cttcgtgaat gttttcatcc gtattttgta
    20701 gcaaggtcac cttaatcaat tcacgcgcat ccaacgcctg acgaacactg gttttaatct
    20761 ggtcatttag accatttttc ccaatctgaa taatgggttt gagactatgt gcctgactgt
    20821 tcaaaaaggc acgttgtttt gaagttagca taatttatta tacaaagggc gttgtaacag
    20881 actgggagac tgttacaatc gggaaataaa gaaaacaaag tttttcctta ttgcgccgat
    20941 atacttagcc cgtgttcaaa tcagaagact tgaacttcct ttctctttaa ttgtaaaaac
    21001 tgaaatcaaa tacctcttat actcaatgaa aattaaaatc agactaggcg acgcagatgc
    21061 agatagaacc ctactacttt cacgctttca ggtagcaggc tgtaaagctc cactggagct
    21121 ttacactcat caaatcaagt caacaacgtc tgcttttaat tttcgaagag tattagataa
    21181 tcgccttgcg aatgaccaca tccacaccct taggagccca ggcagcaatc ttggcttttt
    21241 cattgacacg aatccaacca agtcctgaga agaccacatc cgtcttctcg ttgatggtaa
    21301 attcatgacg gaccagttca ggaaattctt tcaattcctt gctggttggt ggcactaaaa
    21361 gtgagcccgc atgtttctga taaaactcgc tagctccctg caacttggtg cggtggagtt
    21421 ggagctcatt atcaaagaag gccgtaaagc cttggcgttc accagccaca aagtcaaatc
    21481 gtcccagacc agccaagaac aaggtttgtt ctggattgag ctggtaggtc tttggcttga
    21541 tttccttgcg agggctgatg tacttgaggt ttttagccgt caaataatgg gccatctggt
    21601 gacggtggat aattcctggc gtgtcataga tgtatgaacc atcatccaga gggatttcaa
    21661 tcttatccag cgtcgttcca gggaaacgtg aagtcgtaat gacatccttg tcccctgtga
    21721 tttcttgaat aatagcgtta atcaaggttg acttgccgac attggtcacg ccgaccacat
    21781 agacatcccg tcccttgcgg tgttgttcaa tcttttcaat caaatccttg atagcctgct
    21841 tattttgggc tgaagtcaag accacatcaa ccggtcgcat gccgatttca tgtgcccgct
    21901 cggtcaacca ctgagtaacc ttgcctgtct tgacagactt gggtaaaatg tcctgcttgt
    21961 tgccgaccaa aagcacgtca tttccagaaa taaagcgagg taaacctgga atgactgacc
    22021 cattgaaatc aaaaatatca atgacattaa ccaccaaggc atcgctttct cctacgctat
    22081 ggaggagttt caagaaatca tcgtctgaga tattgacatc tgaaatctca ttgtaatggc
    22141 gcaaacggaa acaacgttgg cagtagagtt gccctgtttc caggcctttt tctagggctg
    22201 attgaggtgt aaaaccagcg acagccttgt ctagcgtctg aatctgggca ccacagccga
    22261 tacaaaataa ttcttccact tacatctccc tcttgtagtc tatcgctcca tatttagcga
    22321 tgattttttt catagtccgt cgctcacgcc aacggttaat ctgcgtattg atagagtccg
    22381 tctttatcaa gggtttgacc aagacagact tgagaccagc tcgcttagca gcccgaatat
    22441 ccgtcatcag ctgatcgcca atcataatta cctcatacgg ctggacatca aagcgtttca
    22501 aagcacggtt aatcccaaag gtgaaaggct tgagagcgaa ggcttcaaat tcaatcccaa
    22561 atttttcaac cgcccgtttg acacgttcgt atttattgtt agacaccacc acaacaggaa
    22621 tacctgcgtc ctgcaaatca tgtaaccact ggcgcatctc tggcgtacca tcgggattat
    22681 tccaagcaat caaggtatta tccaagtcaa caaacactac ttttatgcca tgttttttca
    22741 agctttcgac ggtcacatca taagcctttt ccaaggcaaa atccggcatg taattttcta
    22801 aactcactgt cgtctccaaa gttttttatc agataattat accatttttc taagaaaaat
    22861 gcgaatcgat agcagaaaac actcggatca accgagtgtt ttctagaggt aatgtaatgg
    22921 tagttgtgaa ttaaatctga ccttcttcca aaatccaatc tttcggaatg aaaatcatat
    22981 gggttagcaa tcaatcaata tcaagagtaa attttgaaaa agcaaaaccg atttgctcta
    23041 ctcctgactc atcctcatcc aaccccaaaa tggtaagagc atgtccataa tctgcatgac
    23101 tatcatcgaa tagacgccac gcgagagcgc gataaattaa cttcttagca tcatagagac
    23161 tgtaacgctg tctgtactct gaaaatgatt gacaatcctt acctttcggt tctactcctt
    23221 tggttttagc aatggcaatc aagctatcat aattatgata acgcaaagta ctattcaagg
    23281 attgggctaa acgaacagca tagtccaaag ctaaatcact cacctcaaca gccacagttc
    23341 tcaattcttt acgtagggga ttcaatagag acgcagcagt ttctagtctg tataccggac
    23401 tttaacaaac aaaaatgaaa acagccgaac atgtcgactg cttccgtgga gattatatga
    23461 aaaagaataa atatattgta tcttccaatg cttaaacaaa ccttaacttc taaacatttc
    23521 tgaataaact tcgattatta ctctgtaatt tatttcactt tcaaagctac cttaccaaaa
    23581 taatctcgtt ccatgtactt gattgattgg taatgcttct aataacgttg gcacattgtc
    23641 cacagtcaat cctccaccaa tcaaaatctc aattttacct tgtgcatagg cgacaatttc
    23701 ctgtaaccat tccacattgt tcaaggcaga ccctgtcaga ctcccacgcg tcaaaatccg
    23761 tgtcactcca tgttctacca gccaatcaag agcctcgaat tggcgttcgc gaggaatttg
    23821 gtcaaaagcc atgtgaaaga caatctgaca gtcctgcgaa acagcaaaca gtctttccaa
    23881 ggctggttcg tccaaccaat tttcctcggt taacaaacca taaaccagac catctgcccc
    23941 cagttcaatc gctgctttac aatcttcaac catcatttcc acctctgctt catcgtagca
    24001 aaaatctcca ccgcgagggc gaatcatggt catgactgtg gcttcattct catgagccag
    24061 ttgacaaaca tgtttgatca ctccataact cggcgtcgtt ccgccaaccg ctaaattatc
    24121 acacagttca actctttgag cacccagaga aagagctttc accacagcta catgattttc
    24181 ggaacaaaat tctttaatca tctttattcc tttcaagtct tttttcttta
//
LOCUS       DAZQCJ010000022        23808 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000022 DAZQCJ010000000
VERSION     DAZQCJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23808
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.022"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            111..1367
                     /gene="pcsB"
                     /locus_tag="ACHU66_000803"
     CDS             111..1367
                     /gene="pcsB"
                     /locus_tag="ACHU66_000803"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan hydrolase PcsB"
                     /protein_id="HFR3389101.1"
                     /translation="MKKKILATIMLSTVVLSNANYVAVISANDVDSQIAAKNQQISEL
                     TAQQAEAQQQVDAIQGQVDAIVSEQAKLTEENTRLEAESQTLAADIERLSADIVSRDG
                     ALKEQARSAQVDGSASSYINTILDSKSIIDAVSRVNAMREIVSANNRMLEQQKADKEA
                     IVEKQKANQEAITTLAANRQKLEDDAQVLQVRQAELEAAKLNLAVQKATAEDEKNSLL
                     VQKAAAEEAARQAAARQAEYQAQQAALAQQQVASVSAPVVSTPVETTVTETFAAPTQT
                     VSQSTPTVSTPTTSTSSGSGSSAAANNARYDASSYPVGECTWGVKSQLSWVGPYWGDA
                     KQWLASARAEGFSTGSTPQVGAIAVWTGGYYGHVAVVTAVQSSTSIQVVESNYMGRRY
                     IGNHRGGYFNPTTTSEGAVYYIYPPY"
     gene            1470..2435
                     /locus_tag="ACHU66_000804"
     CDS             1470..2435
                     /locus_tag="ACHU66_000804"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR3389102.1"
                     /translation="MAFTDLKLFALSSNQKLAEQVSKKIGIPLGKSSVRQFSDGEIQV
                     NIEESIRGTHVYILQSTSSPVNDNLMEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLQTAGVNRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGDGYVVVSPDHGGVTRARKLAQFLKTPIAIIDKRRSVDKMNTSEVMNIIGDIKDK
                     TCILIDDMIDTAGTICHAADALADAGATAVYASCTHPVLSGPAMDNISKSAIKKLVVL
                     DTIEIAEDRLIDKIEHISTAELLAEAIIRIHEKRPLSPLFEAKSC"
     gene            2526..3704
                     /locus_tag="ACHU66_000805"
     CDS             2526..3704
                     /locus_tag="ACHU66_000805"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFR3389103.1"
                     /translation="MDLLNRFNKNLNRIEVSMIRQFDQSISDVPGILKLTLGEPDFTT
                     PDHVKEAAKAAIDANQSYYTGMAGLLELRQAAAEFVAEKYNLHYNPENEILSTIGATE
                     ALSASLVAILEAGDTVLLPAPAYPGYEPIVNMVGADIVEIDTTANDFVLTPEMLEEAI
                     IEQGDKLKAVILNYPANPTGVTYSREQIQAFADVLRNYPVFVLSDEVYAELTYTGQPH
                     TSIAEFLPEQTILIQGLSKSHAMTGWRIGLIMSQAPIIAQIIKSHQYLVTAASTAMQY
                     GAVEALKNGKDDALPMRAEYVKRRDYIIEKMTDLGFKIIKPDGAFYIFAKIPAGYNQD
                     SFSFLQDFARKKAVAFIPGAAFGQYGEGYVRISYAASMEKIQTAMACLKEYLEENGTN
                     "
     gene            3691..4473
                     /gene="recO"
                     /locus_tag="ACHU66_000806"
     CDS             3691..4473
                     /gene="recO"
                     /locus_tag="ACHU66_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR3389104.1"
                     /translation="MERIETRGLVLYNRNFREDDKLVKIFTEKAGKRMFFVKHASKSK
                     LVASIQPLTYADFIVKINDDGLSYIEDFHQVQPFKNINGDIFKLSYATYILALADAAL
                     QDKVYDPALFAFLVKTLDLMESGLDYEVLTNIFEIQLLGRFGISLNFHECAFCHRVGL
                     PFDYSYKYSGVLCPQHYQQDERRAYLDPNVPYLLDQFQAISFDELETISIKPEMKRKL
                     RFFIDQLYEEYVGIHLKSKKFIDDLSSWGQIMKPRTENEETE"
     gene            4470..5477
                     /gene="plsX"
                     /locus_tag="ACHU66_000807"
     CDS             4470..5477
                     /gene="plsX"
                     /locus_tag="ACHU66_000807"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR3389105.1"
                     /translation="MKRIAVDAMGGDHAPQAMVEGVNQALAAFPDIEIQLYGDEAKIK
                     QYLTATERVSIVHTTEKINSDDEPVKAIRRKKDASMVLATKAVKDGQADAVLSAGNTG
                     ALLAAGVFVVGRIKNIDRPGLMSTLPTMDGKGFDMMDLGANAENTAHHLYQYGILGSF
                     YAEHVRGVKQPRVGLLNNGTEDTKGTPVHQETYKLLAEDKSINFIGNVEARELLNSVA
                     DVVVTDGFTGNAVLKTIEGTAKSIVGQLTGSIKNGGLRAKLGGLLVKPTLKKALGAMD
                     YKTAGGAVLLGLKAPVIKAHGSSDAQSIFYTIKQTRSILEAGIVEKSVAKFSVVEESH
                     D"
     gene            5470..5718
                     /locus_tag="ACHU66_000808"
     CDS             5470..5718
                     /locus_tag="ACHU66_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFR3389106.1"
                     /translation="MTREQVYQRVVELIQDEKGEDFQVQPESTLADNIAADSVEIMEF
                     VLNLEDEFHVDVPDAAIEHFEVLSDIVDFIYEEAKKRS"
     gene            5836..6543
                     /gene="purC"
                     /locus_tag="ACHU66_000809"
     CDS             5836..6543
                     /gene="purC"
                     /locus_tag="ACHU66_000809"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR3389107.1"
                     /translation="MKTDLLYSGKAKDIYATADSDQIVAVYKDQATAFNGGKKEQIVG
                     KGRLNNLISSLIFEKLNEAGVPTHFIKRLSDSEQLNKKVEIIPLEVVLRNVTAGSFSK
                     RFGVEEGITLPTPIVEFYYKKDELDDPFINDEHIAFLELASQDQIAYIKEETRRINEF
                     LKDLFAQIGLTLVDFKLEFGVDSSGQILLADEFSPDNCRLWDADGNHLDKDVFRRGLG
                     ELTEVYEVVLAKLQEVK"
     gene            6556..10275
                     /locus_tag="ACHU66_000810"
     CDS             6556..10275
                     /locus_tag="ACHU66_000810"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR3389108.1"
                     /translation="MAKRIFVEKKADFQIKAEALRKELTHNLQLTSLSSLRLVQVYDV
                     FNLEEDLLEQAIKHIFTEQVTDKVLSDEELGLEGAVYFAIEALPGQFDQRAASSQEAL
                     LLLGSRQEVRVNTGQLYILNGDVQEEELAAIKNYLLNPVDSRFKDMDAPLVTQEFSVS
                     DTTIPSLDFFDSYGVEEFASYKREAGLAMEVEDLLFIQDYFKSIGRVPTETELKVLDT
                     YWSDHCRHTTFETELRSIDFSASKFHKQLQATYDKYLAMRTELGRTDKPQTLMDMATI
                     FGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPFGG
                     AATCIGGAIRDPLSGRSYVYQAMRISGAGDITQPLTATRSGKLPQQIISKTAAHGYSS
                     YGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPVAGDVVILLGGKTG
                     RDGIGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGDVTRLIKKSNDFGA
                     GGVCVAIGELADGLEIDLDKVPLKYAGLNGTEIAISESQERMSVVVRPQDVDTFIAAC
                     RQENIHAVVVAKVTEKPNLVMTWNGQTIVDLERSFLDTNGVRVVVDAKVVDSSVKLPE
                     TRRTSAETLQEDLKDLLSDLNHASQKGLQTIFDSSVGRSTVNHPLGGRHQLTPTESSV
                     QKLPVQQGVTTTASVMAQGYHPYLADWSPYHGAAYAVIEATARLVATGANWSKARFSY
                     QEYFQRMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEDLTVPPTLVAF
                     GVTTADSCKVLSPEFKATGEHIYYLPGQALSEDIDFALIKSNFKAFEKWQSDYVITAA
                     SAVKYGGVLESLALMSFGNQVGARVELADLETSLTGQLGGFVFTSQEDIPDAVKIGQT
                     TTDFTLVVNGVNLSGRDLQVAFEGKLEEVYPTEFEQATELQDVPAVTSSAVIKAKETV
                     EVPVVYIPVFPGTNSEYDSAKAFEQAGAKVNLVPFVTLDAESIEQSVDTMVDNIAKAN
                     ILFFAGGFSAADEPDGSAKFIVTILRNAKVRSAIDQFIEKRGLIIGICNGFQALVKSG
                     LLPYGNFEKAGETSPTLFYNDANQHIAKMVETRIANVNSPWLAGVQVGDIHAIPVSHG
                     EGKFVVTDEEFATLRDNGQIFSQYVDFTGQPSMDSKYNPNGSSHAIEGITSRNGQIIG
                     KMGHSERYEDGLFQNVPGKKDQGLFVSAVRYFTGK"
     gene            10278..11732
                     /gene="purF"
                     /locus_tag="ACHU66_000811"
     CDS             10278..11732
                     /gene="purF"
                     /locus_tag="ACHU66_000811"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009908857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR3389109.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     LANDGGQLRRHRGTGLIAEVFKNPADLEALTGTAAIGHVRYATSGSASINNIQPFLFD
                     FADMQVGLAHNGNLTNAVSLKAELEKNGSIFSSSSDTEILMHLIRRSHNPDFMGKIKE
                     ALNTVKGGFAYLILLEDKLVAALDPNGFRPLSIGKMKNGAWVVASETCAFEVVGADWV
                     RDVEPGEIVVIDDSGIQYDSYTRDTQLAVCSMEYVYFARPDSVIHGVNVHTARKNMGR
                     RLAQEFQHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSSVVKGKRVVMIDDSIVRGTTSRRIVQLLRDAGAKEVHVAIGSPELKY
                     PCFYGIDIQTRRELISANHTVDEVCEIIGADSLTYLSLEGMIEAIGIETDAPKGGLCV
                     AYFDGEFPTPLYDYEEEYLRSLEEKTSFYIENVK"
     gene            11788..12810
                     /gene="purM"
                     /locus_tag="ACHU66_000812"
     CDS             11788..12810
                     /gene="purM"
                     /locus_tag="ACHU66_000812"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR3389110.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERLGVMGALGGFG
                     GMFDLTKLDVKEPVLVSGTDGVGTKLMLAIQYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYIATGKNEPAKLEQVVAGVAEGCVQAGCGLIGGETAEMPGMYGEDDYDLAGFAV
                     GIAEKYQIIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADVSGDALLPELNGRALK
                     DVLLEPTRIYVQQVLPLVKAGLVNGIAHITGGGFIENVPRMFADNLAAEIEEDKIPVL
                     PIFTALEKYGKIKHEEMFEIFNMGIGLVLAVSPDKVDSVCQLVDEEVYTIGRIIAKED
                     KSVVIK"
     gene            12807..13358
                     /gene="purN"
                     /locus_tag="ACHU66_000813"
     CDS             12807..13358
                     /gene="purN"
                     /locus_tag="ACHU66_000813"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR3389111.1"
                     /translation="MKRIAVFASGNGSNFQVIAEQFKVAFVFSDRRNAYVLERAEKLG
                     VPTFAFELKEFSDKQTYEEAIIQLLDQHQIDLVVLAGYMKIVGPTLLAQYEGRIINIH
                     PAYLPEFPGAHGIEDAWNAGVAESGVTVHWVDSGVDTGQIIKQVRVPRLADDTLETFE
                     ARIHEAEYQLYPAVLEELGAVKK"
     gene            13368..14915
                     /gene="purH"
                     /locus_tag="ACHU66_000814"
     CDS             13368..14915
                     /gene="purH"
                     /locus_tag="ACHU66_000814"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR3389112.1"
                     /translation="MTKRALISVSDKNGIVEFAQELTKFGWEIISTGGTKVALDQAGV
                     TTIAIDDVTGFPEMMDGRVKTLHPKIHGGLLARRDLDSHLQAANDHEIGLIDLVVVNL
                     YPFKETILRPDVTYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYPTVLGEIAEQ
                     GETSYATRQRLAAKVFRHTAAYDALIADYFTKQVGEDKPEKLTITYDLNQPMRYGENP
                     QQNADFYQNALPTAYSIAAAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHM
                     NPCGIGQAETIEQAWDYAYEADPVSIFGGIVVLNREVDAATAEKMHPIFLEIIIAPGY
                     SAEALAILTNKKKNLRILELAFDAQDASEVEKEFTGVVGGLLVQDQDVVVESPVDWQV
                     VTERQPSEQEWAAMEFAWKSSKYVKSNGIIITNDKMTLGVGPGQTNRVASVRIAIEQA
                     KDRLEGAVLASDAFFPFADNVEEIAAAGIKAIIQPGASVRDQDSIDMANKYGLTMVFT
                     GVRHFRH"
     gene            14980..15810
                     /locus_tag="ACHU66_000815"
     CDS             14980..15810
                     /locus_tag="ACHU66_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFR3389113.1"
                     /translation="MTLKPFTKWTGGKRKLLTQLHEHLPFEYNRYFEPFVGGGALFFD
                     LAPENAVINDFNEELINTYLQIRDNPEALLDLLHIHQENNSKEYYLDVRSVDRDGRIE
                     TMSDVERAARILYMLRVDFNGLYRVNSKNQFNVPYGRYKNPKIVDSELILSVSRYLND
                     NNILIMQGDFVTAVEEADAGDFVYFDPPYVPITATSSFTSYTHEGFSDQDQRRLRDTF
                     IDLDRRGVNVMLSNSSSPVVEELYAGFNISRVDVSRTNGASNSSRGKIQEIIVRNYGE
                     "
     gene            15800..16615
                     /locus_tag="ACHU66_000816"
     CDS             15800..16615
                     /locus_tag="ACHU66_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR3389114.1"
                     /translation="MANNEYKYGGVLMNKPYYHKNKAILVHADTFEFLNKMKPESMDM
                     IFADPPYFLSNGGISNSGGQVVSVDKGDWDKVDSLEEKHEFNRKWIRLAKNVLKPNGT
                     IWISGSFHNIYSVGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKDD
                     KKARHYYNYELMKELNDGKQMKDVWVGGLTKKSEKWAGKHPTQKPEYLLERIILASTQ
                     EGDYILDPFVGSGTTGVVAKRLGRKFIGIDAERDYLKIARTRLDKEDDKKNFS"
     gene            16593..17519
                     /locus_tag="ACHU66_000817"
     CDS             16593..17519
                     /locus_tag="ACHU66_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HFR3389115.1"
                     /translation="MTKRTFHSWFETFTDTVADWNYYVDFTKVFNNMNDLYLRTNLNI
                     LNTLVGSKQIREDFIAICDKYPDVLTVIPILLAIRLESKGRGKNRKPIALPIREYGDD
                     AIISYDFDFYSPNYAIEDYADLLENTGIFELLQSHLVKNLQDYVYGVEVGLDSNGRKN
                     RMGKIMEALVERVLQNAGLEEKTDYYKQIRTGEIESLFHIDLSSITNNGKNHKKFDFA
                     IHVNGQLYLTEVNFYSKSGSKPNETTRSYENLAEKLTEISNVHFVWITDGEGWQRSKS
                     NLEEAFERIPRLYNLHDIRNKNIKELLEMEMD"
     gene            17519..18424
                     /locus_tag="ACHU66_000818"
     CDS             17519..18424
                     /locus_tag="ACHU66_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HFR3389116.1"
                     /translation="MNLQAYINLSSEERLKIFLSTLSVTNRTPDYYVNWSKVERETKK
                     YELELNTLNYLIGKDDIYNEALQLFNKQPNLLKAIPSLIASRDKTLDILSIDADDNMG
                     FDNLDFAKIDEDRIVDYTNFVEATGLLDFLQKKANRSLVDYVYGVEAGLDSNARKNRS
                     GTTMEGILERTVARVCKEYGLEFKAQATAPFIKDNWGIEVPVDKSERRFDVAVFSKDK
                     HKLWLIETNYYGGGGSKLKAVAGEFTELSQFITKSTDDVNFVWVTDGQGWKTAHLPLA
                     EAFGHITHVFNLDMLKQGYLHDLFR"
     gene            18545..19807
                     /gene="purD"
                     /locus_tag="ACHU66_000819"
     CDS             18545..19807
                     /gene="purD"
                     /locus_tag="ACHU66_000819"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR3389117.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESEQVEQVFVAPGNDGMTLDGIEL
                     INIGISEHSALINFAKENDIAWTFVGPDDALAAGIVDDFEQAGLKAFGPSRLAAELEW
                     SKDFAKQIMVKYGIPTAAFGTFSNFEEAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAREMLLDNKFGDSGARVVIEEFLAGEEFSLFALVNGDQFYILPTAQDHKRAF
                     DGDQGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPILEGMITEGRSYLGVLYAGLILT
                     DQGPKVIEFNARFGDPETQIILPRLTSDFAQNIDDILHKRPTQLTWLNSGVTLGVVVA
                     SNGYPLDYEKGVTLPAKTEGDITTYYAGARFAENSRALLSNGGRVYMLVTTADTVQDA
                     QEKIYSELKNQDTTGLFYRTDIGSKAVK"
     gene            19833..20321
                     /gene="purE"
                     /locus_tag="ACHU66_000820"
     CDS             19833..20321
                     /gene="purE"
                     /locus_tag="ACHU66_000820"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009853194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR3389118.1"
                     /translation="MNIPISIIMGSSSDWKTMKKAADVLDKFGVAYEKKVVSAHRTPD
                     LVFRHAEEARGRGIKVIIAGAGGAAHLPGMVAAKTTLPVIGVPVQSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALTALRILSIEDSGIADQLASFAIEQGKIAEAMSDE
                     LN"
     gene            20308..21393
                     /gene="purK"
                     /locus_tag="ACHU66_000821"
     CDS             20308..21393
                     /gene="purK"
                     /locus_tag="ACHU66_000821"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR3389119.1"
                     /translation="MNSTKTIGIIGGGQLGQMMAISAIYMGHKVITLDPAADCPASKV
                     SEVIVAPYHDVAALKQLAERCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNC
                     IFEKDFLAKKAGVQVAPYKVVTSSLDLEGLDLSKNYVLKTATGGYDGHGQKVIREAAD
                     LEAASQLANSTECVLEEFVNFDLEISVLVSGNGSDYTVFPVQENIHRNNILYKTIVPA
                     RISDELAEKAKTMALQIADKLHLAGTLCVEMFVAGQEILVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHIRGILGEPLPPIRLLSPAVMINVLGQDMEAVQTFLQENPTAHAHFYGKLE
                     AKHNRKMGHVTVLTEDVEVGFRQDEIR"
     gene            21380..22303
                     /locus_tag="ACHU66_000822"
     CDS             21380..22303
                     /locus_tag="ACHU66_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HFR3389120.1"
                     /translation="MKLDRLKEVDVREIWKHEQYDFSEWLSRTENIELLSDEIGLTLT
                     EIDKEVFVGSYRCDLVAIDETTGIKVIIENQLELTDHDHLGKIITYASGLDAEVVIWI
                     VKKAREEHRSAIEWLNNNTTKNISFFLIEIHAYQIGSSMPAPKFVLVEKPNDFTKLAK
                     GNNHGGDLSKAQAERLYFWTQFNNVVATKNKPFNVRKATTDHWYDVAIGTSEAHISIT
                     LVNKSKSVGIEIYINNNKELFDKLSSHADEIHEELGFEMEWKRLDNRKVSRIIYYIKG
                     LDFENHDNYNDLIDEVINKVIILRDVVKKYI"
     gene            22338..23630
                     /gene="purB"
                     /locus_tag="ACHU66_000823"
     CDS             22338..23630
                     /gene="purB"
                     /locus_tag="ACHU66_000823"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR3389121.1"
                     /translation="MIERYTRPEMGNIWSEENKYKAWLEVEILADEAWAELGEIPKED
                     VALIREKATFDIDRILEIEEETRHDVVAFTRAVSESLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAREHKYTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNMERFDVAAKGVEAGKISGAVGNFANIPPFVEEYVCGKLGIRPQEISTQVLPRDL
                     HAEYFSALALIATSIERMATEIRGLQKSEQREVEEYFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVVRGHLVTAFENVALWHERDISHSSAERIITPDTTILINYMLNRFGNIVKNL
                     TVFPENMKRNMESTFGLIYSQRVMLSLIEKGMTREEAYDLVQPKTAQSWDNQVDFKPL
                     LEADERVTAKLSQEEIDELFNPDYYAKRVDDIFERLGL"
BASE COUNT         6810 a         4586 c         5888 g         6524 t
ORIGIN      
        1 tatttggaat aacaaacaag acatagaaat gtaataattg cgtaatatac ttacgcgatt
       61 tttttgataa aatgagagag tcagaaagta tagaggagtt atgttcggat atgaagaaaa
      121 aaatcttggc tacaattatg ttaagtacag tcgttctatc taatgctaat tatgtagctg
      181 tgattagtgc gaatgatgta gatagtcaga ttgcagcaaa aaatcaacag attagtgagt
      241 tgacagcaca acaagctgaa gctcaacaac aagttgatgc tattcaagga caagttgatg
      301 caattgttag tgaacaggcg aaattaacag aagaaaatac tcgtttggaa gcagaatcgc
      361 agacattggc ggcagatatt gagcgtttgt cagctgatat tgtgtcacgt gatggtgctt
      421 taaaggagca ggcgcgtagt gctcaagttg atggttctgc ttcaagttac attaatacaa
      481 ttttggattc aaaatcaatc attgatgctg tttctcgtgt taatgcaatg cgtgagattg
      541 tttcagctaa taaccgtatg ttagaacaac aaaaggctga taaggaagca attgttgaaa
      601 aacaaaaggc aaatcaagag gcaatcacta ctttggcagc taatcgccaa aaattggaag
      661 atgacgccca agtattgcaa gtgcgtcagg ctgaattgga agctgctaag ttaaatttgg
      721 ctgtgcagaa agctactgct gaagatgaga aaaattcatt gttggtgcaa aaggcagctg
      781 cagaggaagc ggctcgtcaa gcggcagctc gtcaagcgga gtatcaagca caacaggcag
      841 ctctagcaca gcaacaagta gcctcagtat cagcaccagt cgtgtcaacg ccggtagaaa
      901 ctacagtgac tgaaacgttt gcagccccta ctcagacagt atcacaatct acaccaacgg
      961 taagcacacc tacaacgtct acatcatcag gttcaggtag ttcagcggca gcaaataatg
     1021 cgcgttatga cgcttcatct tacccagttg gtgagtgtac ttggggagtt aaatctcaac
     1081 tttcatgggt tggtccttac tggggagatg ctaaacagtg gttagcatca gcacgtgctg
     1141 aaggttttag tacaggttct actccacaag taggtgcgat tgctgtttgg acaggtggtt
     1201 attatgggca cgtagctgtt gttacggctg ttcaatcttc aacaagtatc caagttgttg
     1261 aatcaaacta catgggtcgt cgttatattg gtaaccatcg tggtggttat ttcaatccaa
     1321 caacgacttc tgagggagca gtttactaca tttaccctcc atattaagaa attttcaaat
     1381 agagcgtgtg gatttgcgct ctatttttgt ttgaaaaatg aaggaaaaag ggttaaaata
     1441 gtaaaggtag aaatatatag gaggctgtca tggcgtttac tgacttaaaa ttgttcgctc
     1501 tttcgtcaaa tcagaagtta gctgaacagg tgtcgaaaaa aataggaatt cctctgggaa
     1561 aatcaagtgt tcgtcaattt tcagatggtg agattcaggt aaatattgag gaatccattc
     1621 gtggaactca tgtgtatatt cttcaatcca ctagctcacc agttaatgat aatttaatgg
     1681 aaattttgat tatggtcgat gctcttaaac gtgcatctgc tgaatcagta aacgtagtaa
     1741 tgccttatta tggttatgca cgccaagacc ggaaggctcg tgcacgggaa ccaattacat
     1801 caaaattggt ggctaatatg ttgcagacag cgggggtcaa tcgtttgttg acaattgatt
     1861 tgcatgctgc gcagattcag ggattcttcg atattcctgt tgatcatttg atgggtgctc
     1921 cattgattgc ggattatttt gagcgtcgtg gtatggtagg tgatggctat gttgttgtat
     1981 cccctgacca tggtggtgtg acacgtgccc gtaagttagc acagttcttg aagacaccga
     2041 ttgctattat tgataaacgc cgtagtgttg ataagatgaa tacatcagaa gtgatgaaca
     2101 ttattggcga tattaaggat aaaacttgta tcttgattga tgatatgatt gatactgcgg
     2161 gtaccatctg tcatgcagcg gatgctttgg cagatgcagg ggcaacagct gtttatgctt
     2221 catgtacgca cccagtattg tcagggcctg ctatggataa cattagtaag tcagcaatta
     2281 agaaattggt tgttcttgat acaattgaaa ttgcagaaga tcgcttaatc gataaaatcg
     2341 aacatatttc aacggcagaa ttattagcgg aagcgattat tcgtattcat gaaaaacgtc
     2401 ctttgtcacc tttgtttgag gcaaagtcgt gttaaatagg tatgagaaga gtcaaatgac
     2461 tcttcttttt tacatttatt ttcaaaatgt attataatag ttttatatgt ttttgaggtg
     2521 gcagaatgga tttattgaat cgttttaata agaatttgaa tcggattgag gtttcgatga
     2581 ttcgtcagtt tgatcagtct atttcggatg ttcccgggat tttgaaactg accttgggag
     2641 agcctgattt tacaacgcct gatcatgtaa aagaagcagc taaggctgct atcgatgcta
     2701 accagagtta ttatacaggt atggctggtc ttttggagtt gcgccaagct gcagctgagt
     2761 ttgtggctga aaaatataat ttacactaca atccagaaaa tgaaatcctc tctacaatcg
     2821 gtgcgacaga agctctgtca gcgagtttag ttgctatttt ggaggctggg gatacagttc
     2881 ttctgcctgc ccctgcctat cctggttatg agccgattgt caatatggtg ggagcagaca
     2941 tcgttgaaat cgatacgacg gcaaatgatt ttgtattgac acctgagatg ttggaagagg
     3001 cgattattga gcagggtgat aagctaaaag ctgtcattct caactatcct gccaatccaa
     3061 caggtgtgac ctactcacgt gagcagattc aggcttttgc ggatgtcttg cgtaactatc
     3121 ctgtctttgt cctatcagat gaggtctatg cggagttgac ttacactggt cagcctcata
     3181 cttcgattgc ggagtttttg ccagaacaga ccattttgat tcagggattg tccaagtccc
     3241 atgctatgac aggttggcgg attggcttga ttatgagtca ggctcccatc atcgctcaaa
     3301 ttatcaagag ccatcagtat cttgtgactg cagcgtcaac tgctatgcag tatggtgcgg
     3361 ttgaggcctt gaaaaatggt aaggatgacg ctcttccgat gcgggcggag tacgtcaagc
     3421 gtagggatta catcatcgag aagatgacgg acttgggctt taaaattatc aagcctgatg
     3481 gagcatttta catctttgca aaaattcctg cgggttacaa tcaagattcc tttagcttct
     3541 tgcaagactt tgcgagaaag aaagcggtgg cctttattcc aggcgcagcc ttcggtcagt
     3601 atggagaagg ctatgtgcga atttcctacg cagcaagtat ggagaagatt caaacagcca
     3661 tggcttgttt gaaggaatat ctagaagaaa atggaacgaa ttgaaaccag gggattagtc
     3721 ctatataatc ggaattttcg agaagatgac aagctggtca agatttttac agagaaggct
     3781 ggcaagcgaa tgtttttcgt gaaacatgcc tctaaatcca agctggtagc ttctatccag
     3841 cctttgacct atgcggattt tatcgttaaa atcaatgatg atgggctgtc ttatatcgaa
     3901 gattttcatc aggtacagcc ctttaagaat attaacggtg atattttcaa gcttagctat
     3961 gctacctata tcttggcctt ggccgatgcg gccttgcagg acaaggtcta tgacccagct
     4021 ctctttgctt ttttggtcaa gaccttggac ttgatggagt caggtttgga ctacgaagtt
     4081 ctgaccaata tctttgaaat tcagctcttg ggtcgatttg ggatcagtct gaattttcac
     4141 gagtgtgctt tttgtcatcg ggttggcttg ccttttgact attcctacaa gtacagcggt
     4201 gtcttgtgtc cgcaacacta tcaacaagat gagcgacggg cttatctgga tccaaatgtt
     4261 ccctatctac ttgatcaatt tcaggctatt tcctttgatg agctggaaac catttccatc
     4321 aagcctgaga tgaagcgaaa attacggttt tttattgacc agctgtacga ggaatatgtg
     4381 gggattcact tgaaatccaa gaaatttata gatgatttgt cttcttgggg gcagattatg
     4441 aaaccaagaa cagaaaatga ggaaacagaa tgaaacgtat tgcagtagat gctatgggtg
     4501 gagaccacgc ccctcaggca atggtagaag gtgtcaatca agccttggct gcctttccag
     4561 acattgaaat tcaactttat ggtgatgagg ctaaaatcaa gcagtatttg acagcgacag
     4621 agcgtgtcag catcgtccat acgacggaga aaatcaattc agatgatgag cctgtcaagg
     4681 cgattcgtcg taagaaagat gcttcgatgg tcctagcgac caaggctgtc aaggatggtc
     4741 aggcagatgc ggtcttgtca gctggaaata caggtgctct tttggcggca ggcgtctttg
     4801 tcgtcggtcg catcaagaac atcgaccgtc ctggtcttat gtctaccttg ccgactatgg
     4861 acggtaaggg atttgatatg atggatttgg gggcaaatgc tgaaaataca gctcatcacc
     4921 tctatcagta tggtattctt ggctcatttt acgcggagca cgtgcgtggt gtcaaacaac
     4981 ctcgtgtggg acttttgaac aacggtacgg aagataccaa gggcacgcca gttcaccaag
     5041 aaacctataa actcttggcg gaagacaagt caatcaactt tatcggtaat gtggaggcgc
     5101 gtgagttgct caacagtgtg gcagatgtgg tcgtgacgga tggtttcacg ggaaacgctg
     5161 tgctgaaaac cattgaggga actgcaaaat ccatcgttgg tcagttgact ggctcaatca
     5221 agaatggcgg tctgcgtgct aaattgggag gcttgttggt caaaccaacc ttgaaaaagg
     5281 ccttgggggc tatggactat aaaactgcag gtggtgctgt cttgcttggt ctgaaagcac
     5341 ctgtcatcaa ggcgcacgga tccagcgatg cccagtcgat tttctatacg attaagcaga
     5401 cgcgttcgat tttggaggct ggcattgttg aaaaatcagt ggccaaattt tcagtagtgg
     5461 aggagagtca tgactagaga gcaggtctat cagcgggttg ttgaattaat tcaggatgaa
     5521 aagggagaag attttcaagt tcaacctgaa tctactttgg cagataatat tgcagcagat
     5581 tcagtagaaa tcatggaatt tgttcttaat ttggaggatg aatttcatgt agatgttcca
     5641 gatgctgcta ttgagcattt tgaggtccta tctgatattg ttgattttat ttatgaagaa
     5701 gcaaagaaac gttcgtagtt tacgggcgtt tttctttgtt ttttcctaaa aaaccgtaaa
     5761 tttgttgtga atgcgaatat tgtgtgttag aataaacgaa aagactagaa tattttacga
     5821 aaggcgaaca ttctcatgaa aacagacctt ctctattcag gaaaagccaa agacatctac
     5881 gctacggctg atagcgacca gattgttgcg gtttataagg atcaggcaac ggcttttaat
     5941 ggtggtaaga aagaacagat tgtgggcaag ggccggctca ataatctgat ttcatctttg
     6001 atttttgaaa aattgaatga agctggtgtc ccaacgcatt ttatcaagcg tttgtcggat
     6061 tcggagcagt tgaataagaa ggtggagatt attcctcttg aggttgtttt gcgaaatgtg
     6121 acagctgggt ctttctcaaa acgcttcggt gtggaagaag ggataacctt acctactcct
     6181 atcgtggaat tttactataa aaaagatgag ttggacgatc cttttatcaa cgatgaacac
     6241 attgcctttc tagaactggc tagtcaggac caaatcgcct atatcaagga agaaacaaga
     6301 cgaatcaatg aattcttgaa ggacttgttt gctcagattg ggctgacttt ggtggacttc
     6361 aagctagaat ttggggttga ctcgtctggt cagattttat tggcggatga gttttcgcct
     6421 gataattgtc gtttgtggga tgcggatggc aaccatctgg acaaggatgt tttccgtcgg
     6481 gggctcgggg agttgaccga ggtttacgag gtcgtattgg caaagttaca agaagtgaaa
     6541 taaggattgg aaaatatggc aaagcggatt tttgttgaga agaaggcgga ttttcagatt
     6601 aaggcggagg ctcttcgcaa ggagttgact cataatttac agttgacaag cctgtcaagt
     6661 ttgcggttag tgcaggttta cgatgtcttc aatcttgagg aggacttgtt agagcaggcc
     6721 atcaagcata tctttacaga gcaggtgacg gacaaggttt tgtcggatga agaattaggt
     6781 ctggaaggtg cagtttattt tgctattgag gctttgcctg ggcagtttga ccagcgggct
     6841 gccagcagtc aggaggccct tctcttattg ggcagtcgtc aggaggtgcg tgtcaataca
     6901 ggtcagctct atattttgaa cggtgatgtg caggaagaag aattagctgc catcaagaat
     6961 tatttgctca accctgtgga ttcgcgattc aaggatatgg acgctccttt ggtgactcag
     7021 gagttttcag tttcggatac tactattcca agcttggact tttttgatag ctacggggta
     7081 gaggaatttg cgtcttacaa gcgtgaggct ggtttggcta tggaagtgga agacctgctc
     7141 ttcattcagg attacttcaa gtcaattggt cgagtgccaa ctgagacaga actcaaggtc
     7201 ttggatactt actggagtga ccactgccgc catacgacat ttgagacaga acttaggtct
     7261 attgactttt cagcttcaaa attccacaag caattgcagg cgacttatga caagtatctg
     7321 gctatgcgaa cagagttagg tcggacagac aaaccgcaga cgcttatgga tatggcgacg
     7381 atttttggtc gctatgagcg agcaaatggt cgtctggatg acatggaagt gtcagatgaa
     7441 atcaatgctt gctcggtgga aatcgaagta gatgtggatg gcgtgaaaga gccatggctc
     7501 ctcatgttca agaatgaaac ccataatcac ccaacagaaa tcgagccttt tggtggtgcc
     7561 gcaacctgta tcggtggagc cattcgtgat cctttgtcag gtcgttctta tgtttatcaa
     7621 gccatgcgga tttcaggtgc aggcgatatt acccaacctc tgacagctac tcgctcagga
     7681 aaattgccac agcaaatcat ttcaaaaaca gcggcacatg gttattcttc atacggaaac
     7741 caaatcggtt tggcgacaac ttatgtacgt gaatatttcc acccaggctt tgtcgcaaaa
     7801 cgtatggaat taggtgccgt tgtcggtgct gctcctaagg aaaatgtggt tcgtgaaaaa
     7861 ccagtcgcag gcgatgtggt tatcttgtta ggaggcaaaa caggtcgaga tggtatcggt
     7921 ggggcaacag gctcgtccaa ggtacagaca gttgagtctg tggaaacagc tggtgcggaa
     7981 gtccaaaaag gaaatgccat tgaagaacgc aaaatccaac gcttgttccg aaatggtgac
     8041 gttacacgcc tgataaaaaa atccaatgac ttcggtgcag gcggtgtctg cgtagctatt
     8101 ggggaattgg cggacggtct tgaaatcgat ttggataagg ttcctctaaa atatgcaggc
     8161 ttgaatggaa cggaaatcgc tatttctgaa tcacaggagc ggatgagtgt cgttgttcgt
     8221 cctcaagatg tcgatacctt catcgcagcc tgccgtcagg aaaatatcca tgcggttgtt
     8281 gtagccaaag tgacggaaaa accaaatctg gtcatgactt ggaatggtca aaccattgtc
     8341 gacttggaac gttccttcct tgataccaac ggtgtgcgcg tggtggtgga tgccaaagtg
     8401 gttgacagtt ctgtcaagct accagaaaca cgtagaactt ccgctgaaac gctacaggag
     8461 gacttgaaag accttctatc agacctcaac catgctagcc agaaaggttt gcagacgatt
     8521 ttcgactcat ctgttggtcg ttcaaccgtc aaccacccac tcggaggtcg tcaccagctg
     8581 actccgacag aaagttcggt gcaaaagttg cctgtccaac agggtgtgac gacgacagca
     8641 tcagtcatgg ctcagggtta ccatccttac ctagcagact ggtcacctta tcacggagcg
     8701 gcttatgcag tcatcgaagc gacagctcgc ttggtggcaa caggtgctaa ctggtccaag
     8761 gctcgcttct cctatcagga gtatttccag cggatggaca agcaggctga gcgttttggt
     8821 cagccagtag cggctttgct tggttctatc gaggctcaga ttcagcttgg cttgccgtct
     8881 attggtggca aggactccat gtctggtacc tttgaggact tgacagttcc gccgaccttg
     8941 gttgcctttg gcgtgaccac ggcagacagc tgcaaggttc tatcacctga gttcaaggcg
     9001 actggcgagc atatctatta cctaccaggc caagccttat cagaagatat tgattttgcc
     9061 ttgatcaagt ctaattttaa ggcttttgaa aaatggcaga gcgattatgt gattacggct
     9121 gctagtgcag tcaagtatgg aggagttcta gaaagtctag cccttatgtc ctttggtaac
     9181 caagtcggag caagagttga gcttgccgac cttgaaacta gcttgacagg tcaacttggt
     9241 ggctttgtct tcacatcgca agaagacatt ccagatgctg tgaaaatcgg tcaaaccact
     9301 acagacttta cactggttgt caatggtgtc aacctttctg gacgggactt gcaagtagct
     9361 tttgagggca aactagaaga agtttaccca acagagtttg aacaggcgac ggagttgcag
     9421 gatgttccag cagtcacaag ttcagcggtt ataaaagcaa aagaaaccgt tgaagtgcct
     9481 gtagtttaca ttccagtttt cccaggtacc aactcagaat atgattctgc caaggccttt
     9541 gaacaggctg gtgcaaaagt caatcttgtt ccatttgtga ccttggatgc agagagcatt
     9601 gaacagtctg tggacactat ggttgacaat attgctaagg cgaatattct attctttgca
     9661 ggtggattct cggctgcgga tgaaccagat gggtctgcta agtttatcgt gactatctta
     9721 cgaaacgcca aggtccgctc tgctattgac caatttatcg aaaaaagagg cctcatcatc
     9781 ggtatctgta atggtttcca ggcccttgtc aaatcgggct tgttgccgta tggaaacttt
     9841 gagaaggcgg gtgaaaccag tccaaccctc ttctacaacg atgccaacca acacattgct
     9901 aaaatggtgg aaacgcggat tgccaatgtc aactcaccgt ggttggcagg tgtccaagtc
     9961 ggcgacattc acgctatccc agtttcccac ggtgaaggaa aatttgtagt gacggacgag
    10021 gaatttgcta ctttgcgtga taatgggcaa atcttcagcc aatacgttga ctttaccggt
    10081 cagccaagca tggattctaa gtacaatcca aatggatcca gccatgccat tgagggcatt
    10141 accagtcgca acggtcagat tatagggaaa atggggcatt ccgagcgtta cgaggacggt
    10201 cttttccaaa atgttccagg taagaaagat caagggctct ttgtttcagc agttcgctat
    10261 tttacaggaa aataaatatg acatacgaag ttaaatcact caacgaagaa tgcggtgttt
    10321 tcggcatttg gggacatccg caggctgctc aggtgaccta ttttggtctc catagtttgc
    10381 agcaccgtgg acaagaagga gcagggattt tagccaatga tggcggacaa ttgcgtcgcc
    10441 atcgcggaac aggcctgatt gcagaagtct tcaaaaatcc agcagattta gaagccttga
    10501 caggaacggc tgctattggt catgtccgct atgcaacttc tggctctgct tctatcaata
    10561 atatccagcc cttcctattt gattttgcag atatgcaggt gggtttggca cacaatggga
    10621 atttgaccaa cgcggtcagt ttgaaggctg aacttgaaaa aaatggttcc attttctctt
    10681 cttcttctga cactgagatt ctcatgcact tgattcgccg cagtcacaat ccagacttta
    10741 tgggaaaaat caaggaagcc ctcaatactg ttaaaggggg ctttgcctac ctgatcttgt
    10801 tggaagacaa attggttgct gccttggacc ctaacggttt ccgtccactt tcaataggca
    10861 aaatgaaaaa tggtgcctgg gtagttgcca gcgaaacttg tgcttttgaa gtagtggggg
    10921 ctgactgggt gcgagatgtg gagcctggtg aaattgtcgt cattgatgat tcaggaattc
    10981 aatatgatag ctatacaaga gatacgcaac tggctgtttg ctctatggag tatgtctatt
    11041 ttgctcgccc agattcggta attcacgggg tcaatgtcca cacggctcgt aaaaatatgg
    11101 gtcgtcgttt ggcccaggaa tttcaacatg aagcagatat cgtggttggt gtgccaaact
    11161 cctccctatc tgcggctatg ggatttgcag aggaatctgg tttgccaaat gaaatggggc
    11221 tgattaaaaa ccaatacacc cagcggacct ttattcaacc aacccaggaa ttgcgggagc
    11281 agggtgtgcg catgaagttg tcagccgttt caagtgttgt caaaggcaag cgtgtggtca
    11341 tgattgacga ctctattgtt cgcggtacaa ctagccgccg cattgtccag cttcttcgtg
    11401 atgcaggggc aaaagaagtc catgtggcta tcggcagccc agaactcaag tacccgtgtt
    11461 tctatggtat tgatattcag acccgtaggg agctgatttc agccaaccat accgttgatg
    11521 aagtctgtga gattatcggg gcagacagtc tgacctacct ttctcttgag gggatgattg
    11581 aagccatcgg catcgaaacc gatgcgccaa aaggcgggct ctgtgtagcc tactttgacg
    11641 gagaatttcc aacgcctctc tatgactatg aggaagaata ccttcgtagc ctagaagaga
    11701 aaacgagttt ctacattgaa aatgtcaagt aaagacagag gtgtcaacgt tacttgacag
    11761 acctgtaaac tgttgaaagg aatcaaaatg acaaataaaa atgcctacgc ccaatcgggc
    11821 gttgacgtcg aagcgggata tgaagttgtc gaacgcatca agaagcatgt agctcggaca
    11881 gaacgcttgg gtgtgatggg agccctcggt ggttttggcg ggatgtttga tctaaccaaa
    11941 ctggatgtca aagagccagt cttggtatct gggacagacg gtgtgggcac caagctcatg
    12001 ctggctatcc agtacgacaa gcatgacacc attggtcaag attgcgtggc tatgtgtgtc
    12061 aacgacatta tcgcagcagg tgcagagccg ctttacttcc ttgactacat tgcgactgga
    12121 aaaaatgagc cagccaagct ggagcaagta gtagccggtg ttgcagaagg ctgtgtccaa
    12181 gctggttgcg gattgattgg cggtgaaacg gctgaaatgc ctggtatgta cggcgaggat
    12241 gactatgacc tggctggctt tgctgtcggt attgcggaga agtatcagat tatcgacggt
    12301 agcaaggtca aggagggtga cattctcctc ggcctagctt ccagcggtat ccactccaac
    12361 ggatattccc tagtccgtcg tgtttttgca gatgtttctg gggacgctct gttgccagag
    12421 ctcaatggca gagctcttaa ggatgtccta ttagagccga cacgtatcta tgtccagcag
    12481 gtgttgcctc tggtaaaagc aggtctggtc aacggcattg cccatatcac aggcggtgga
    12541 tttatcgaaa atgtgcctcg tatgtttgcg gacaaccttg cagctgaaat tgaagaagat
    12601 aagattccag tcctcccaat ctttacggcc cttgaaaaat acggaaagat caagcatgaa
    12661 gaaatgtttg aaatcttcaa tatgggtatc ggattggtcc tggcagtcag tccagacaag
    12721 gttgacagtg tctgtcaact agttgacgag gaagtttaca ccattggtcg catcatcgcc
    12781 aaggaagata agagtgtggt catcaaatga aacgaatagc agtgtttgca tcaggcaacg
    12841 gctccaattt ccaagtcatc gcagaacagt ttaaagtagc ttttgtcttt tcagaccgca
    12901 gaaatgccta tgtcttggaa cgagctgaaa aactaggtgt accaaccttt gcttttgaac
    12961 taaaagagtt ttcagataag cagacctacg aagaagccat tatccaacta ttagaccagc
    13021 accagattga cttggtggtc ttggcaggct atatgaaaat tgtgggtccg accttgttag
    13081 ctcaatatga aggtcgtatc atcaatatcc acccggctta tttgcctgaa tttccaggag
    13141 ctcacgggat tgaagacgct tggaatgcag gtgtggcaga aagtggtgtg acagttcact
    13201 gggttgacag cggtgttgac acaggacaaa tcatcaaaca agtccgagtg ccccgactgg
    13261 ccgatgatac cctggaaacc tttgaagcca gaatacatga agcggagtac caactctatc
    13321 cagcggtttt ggaggagttg ggggcagtga aaaaataagg agaaatcatg acaaaacgtg
    13381 cgttaattag cgtatcagat aaaaatggta ttgtagaatt tgcccaagaa ttgaccaagt
    13441 ttggttggga aattatctcg accggtggta ccaaggttgc cttggatcag gctggtgtaa
    13501 ctaccatcgc cattgacgat gtgactggtt ttcctgagat gatggacggc cgtgttaaga
    13561 cgcttcatcc gaaaatacac ggtggtttgt tagcacgtcg ggatttggac agccacctgc
    13621 aagcagctaa cgaccatgaa ataggcttga ttgacttggt agtggttaac ctttacccct
    13681 tcaaagagac tattttacgt ccagatgtga cctatgattt ggcggtggag aacatcgaca
    13741 tcggcggacc gtccatgctt cgttctgcag ccaaaaacca cgccagcgtg accgttgtgg
    13801 tggatccggc agattatccg acggttttag gggaaattgc agagcagggg gaaacgagct
    13861 atgcaacacg tcagcgattg gctgcaaagg tattccgtca taccgcagct tatgatgccc
    13921 taatagcaga ttattttacc aagcaagtag gcgaagataa gcctgaaaaa ttaaccatta
    13981 cttatgacct caatcagcct atgcgttacg gtgaaaatcc ccagcaaaat gcggatttct
    14041 accaaaacgc ccttccgaca gcctattcta ttgcagctgc taagcagtta aatggtaagg
    14101 agttgtcttt caacaacatt cgtgatgcgg atgcggccat tcggattatc cgtgatttta
    14161 aggaccgtcc aactgttgtg gctctcaaac acatgaaccc ttgtggtatc gggcaggcag
    14221 aaaccattga acaggcttgg gattatgctt atgaggctga cccagtatcg attttcggag
    14281 gcatcgtcgt gctgaacagg gaagtggatg ctgcgacggc tgaaaagatg cacccgattt
    14341 tcttagaaat catcatcgca ccgggctact cggcagaagc gctagctatt ttgaccaata
    14401 aaaagaaaaa tcttcggatt ttggagttgg cctttgacgc acaggatgca agcgaagtgg
    14461 aaaaagagtt cacaggcgtt gtcggcggac tcttggtgca ggatcaggac gtggtggtgg
    14521 aaagcccagt ggactggcag gtggtgaccg agcgtcaacc gtccgagcaa gagtgggcag
    14581 ccatggagtt cgcttggaag tcctctaaat atgtcaagtc caacggcatc atcatcacca
    14641 atgacaagat gactttgggc gtggggccgg ggcaaaccaa ccgtgtagcg tccgtccgta
    14701 tcgctatcga gcaagccaag gaccgtttgg agggagccgt tttggcgtcg gacgccttct
    14761 tcccatttgc tgataacgtg gaagaaattg cagctgcagg tatcaaggct atcatccagc
    14821 caggtgcttc tgtccgtgac caagactcca ttgacatggc caacaagtac ggcttgacca
    14881 tggtctttac aggagtcaga cattttagac attgagaatt ttggataagt atagcacccc
    14941 agctggagaa gttggggtgg attttggtat aatagttcca tgactttaaa accttttacg
    15001 aaatggacag gtgggaagcg gaagctatta actcagctac atgaacatct accatttgaa
    15061 tacaatagat attttgaacc gtttgttgga ggaggggcat tattctttga tttagctccc
    15121 gaaaatgccg ttataaatga ttttaatgag gaattgataa atacttattt acagatacgg
    15181 gataatccag aagcattgct tgatttatta catatccatc aagagaacaa ttccaaggaa
    15241 tattatttgg atgttcgttc ggttgacaga gatggtagaa tagaaactat gtccgatgtg
    15301 gagcgagcag cacgcatact atatatgcta cgagtagatt ttaatggtct atatagggtg
    15361 aactctaaaa atcagtttaa tgtcccttac ggcaggtata aaaatccaaa gatagtagac
    15421 tcggaactta ttctaagtgt atcaagatac ttaaatgata acaatatttt aattatgcaa
    15481 ggtgattttg ttacggcagt tgaagaagct gatgcgggag attttgtata ctttgatcct
    15541 ccgtatgttc ctataacggc tactagctct tttacttctt atacgcatga aggtttttct
    15601 gatcaggatc agcgtcgatt aagagatact ttcatagatt tagaccgaag aggagttaat
    15661 gtaatgcttt ctaattcttc aagcccagta gttgaagaat tgtacgctgg ttttaatatt
    15721 tcccgagtag atgtatcgag aacgaatgga gcaagtaatt ccagtcgtgg aaaaattcaa
    15781 gaaattattg taagaaatta tggcgaataa cgaatataag tatggagggg tcttaatgaa
    15841 caaaccatac tatcataaaa acaaggctat tttggtacac gcagatacat ttgaattttt
    15901 gaataaaatg aaaccagaga gcatggatat gatttttgca gatcctccat attttttaag
    15961 taatggagga atttctaact ctggaggtca agttgtttct gtagataagg gggattggga
    16021 taaagttgat tctttagaag aaaaacatga atttaatagg aaatggattc gcttagccaa
    16081 aaatgtgttg aaaccaaatg ggaccatttg gatttcaggg agctttcaca atatttattc
    16141 agtgggtatg gccttagaac aagaaggttt taaaatacta aataatatta cttggcagaa
    16201 gacaaatcct gcaccaaatc tttcctgtcg ttactttact cattcaacgg aaactatttt
    16261 atgggctcga aaagatgata aaaaagctcg tcattattat aattacgaac ttatgaaaga
    16321 actcaatgat ggcaaacaaa tgaaagatgt ttgggttgga ggactaacaa aaaaatctga
    16381 aaaatgggca ggtaagcatc cgactcagaa accagaatat ctcttagaac gtattatttt
    16441 agcttcaact caagaaggag attacatact tgatccattt gtaggaagcg ggacgacagg
    16501 agtggttgcc aaacgtttgg gaagaaaatt tattgggatt gatgctgaac gggattattt
    16561 aaaaatcgcg agaactagat tggataagga agatgacaaa aagaactttt catagttggt
    16621 ttgaaacttt tacagatacg gttgctgatt ggaactacta tgttgacttt acaaaagttt
    16681 ttaataatat gaatgacctg tatttgagaa ctaatttgaa tatcttgaat acgttggtag
    16741 ggagtaaaca aattcgagaa gattttatcg caatatgtga caagtatcca gatgtgttaa
    16801 ctgtcatacc aattcttttg gctatacgtt tggaatctaa aggccgagga aaaaatcgta
    16861 aaccaattgc tttacctatt cgcgagtatg gggacgatgc tataataagc tacgattttg
    16921 atttttattc tcctaactat gctatagagg attatgccga tttactagag aatacaggta
    16981 tttttgaact actgcaatca catctagtta aaaacttaca ggactatgtt tacggtgtag
    17041 aagtcgggtt agattcaaat ggtcgtaaaa atagaatggg taagataatg gaagctttgg
    17101 ttgaaagagt acttcagaat gcaggactag aagaaaaaac ggactactat aaacaaatac
    17161 gaactgggga aattgaatca ctatttcata ttgatctaag ttctataacg aataatggta
    17221 aaaatcataa gaaattcgat tttgcaattc acgtaaatgg tcaattgtat ttaacggaag
    17281 taaatttcta ttctaaatca gggtcaaaac caaatgaaac cacacgttca tatgaaaatt
    17341 tagcagaaaa acttactgaa atttcgaatg ttcattttgt ttggataaca gatggtgaag
    17401 gttggcaaag gtctaaatct aatttagagg aggcatttga aagaatacca agattgtaca
    17461 atctccatga tattcggaat aaaaatatta aagagttatt agaaatggag atggactaat
    17521 gaatttgcaa gcttatataa atttatcttc tgaagaacgc ttaaaaatat ttttaagtac
    17581 tttatcagtt actaatcgaa cgccagatta ctatgtcaat tggtctaagg ttgaacgaga
    17641 aacaaaaaaa tatgagttag aattaaatac tttgaattat ttaattggta aagatgatat
    17701 atataacgaa gcgttgcaac tttttaataa acaaccaaat ttattaaaag ctataccaag
    17761 tttgattgca agtcgtgata aaacactgga tattttatca attgatgccg atgataatat
    17821 gggttttgat aacttagatt ttgcaaaaat tgatgaggat agaattgtag attatactaa
    17881 ctttgtggaa gcaacaggat tattagattt tttgcaaaag aaagccaatc gttctttagt
    17941 ggactacgtt tatggagtag aggcgggact tgacagtaat gcacgtaaaa acagaagtgg
    18001 tactaccatg gaggggattt tagagcgaac agtagcacgt gtctgtaaag aatatggatt
    18061 ggagtttaag gcacaagcaa cagctccatt tataaaggat aattggggga ttgaggtccc
    18121 tgttgataag tcggaacgcc gatttgatgt tgctgtattt tcgaaagata aacataaatt
    18181 atggttaatt gaaacgaatt attacggtgg aggcggtagc aagttaaagg ctgtagctgg
    18241 agaatttaca gaattaagtc aattcatcac aaaatctaca gatgatgtta attttgtgtg
    18301 ggtaactgat ggtcagggat ggaagactgc ccatcttcct ttagcagaag catttggtca
    18361 tataacccat gtcttcaatc ttgacatgct aaaacaaggt tacttgcatg atttgtttag
    18421 ataatcaagt ttgctcacct cgtgtgagct ttttccattc ccgaacattc tcctgttttt
    18481 tctattaaaa tccgtctgtt tttgctatac tgtttataga aaatccgttt ttgaggtgta
    18541 agaaatgaaa cttttggttg tcgggtctgg tggtcgtgaa cacgctattg caaagaagtt
    18601 gttagagtct gagcaggtag aacaggtctt tgttgctcct ggaaatgatg gaatgacttt
    18661 agacggtatt gaattgatca atatcggtat ttccgaacat tctgccctca tcaactttgc
    18721 aaaggaaaat gatattgcat ggacttttgt tggtccagat gatgctctgg cagcaggtat
    18781 cgttgatgat tttgaacagg ctgggttaaa ggcatttggt ccgagtcgtc ttgccgcgga
    18841 gctagagtgg tcaaaagact ttgctaagca aatcatggtc aaatacggca ttccaacagc
    18901 agcctttggc acattttcca acttcgaaga agccaaagcc tacatcgaag agcagggtgc
    18961 accaatcgtg gtcaaggcgg acggattggc actgggcaag ggtgtggtcg tggcggaaac
    19021 cgtcgagcag gcggtcgaag cggcacggga gatgctcttg gacaacaagt tcggtgactc
    19081 aggtgcccgc gtggtcatcg aggagttctt ggcgggcgag gagttttccc tctttgccct
    19141 ggtcaacggc gaccagtttt atatcctgcc gacagcccag gaccacaagc gtgcctttga
    19201 cggagaccag gggcccaaca ctggcggtat gggggcttac gctcctgttc cccacctgcc
    19261 tcaaagcgta gtggacacag cggttgacac tattgtcaag cctattcttg agggcatgat
    19321 tacggaaggg cggtcttatc tgggcgtgct ctatgctggc ttgattttga ccgaccaagg
    19381 tcccaaggtc atcgagttta acgcccgttt tggcgaccca gaaacccaga ttattctgcc
    19441 tcgtctgacc tctgactttg ctcagaacat cgacgacatc ctccacaaac gtccgacaca
    19501 gctgacctgg ctgaatagcg gcgtgacgct gggtgttgtc gtggcctcaa atggctatcc
    19561 tttggattac gaaaaaggcg tgaccttgcc agcgaagacc gagggtgaca tcacgaccta
    19621 ttatgcaggg gctcgttttg cggaaaatag cagagcactg ctttcaaacg gcggtcgagt
    19681 ttacatgcta gtcaccacag cagacaccgt ccaagacgcc caggaaaaaa tttactcgga
    19741 gctgaaaaat caggatacta caggcctctt ttatcgaaca gatattggaa gcaaggccgt
    19801 taaataagga acaaagaaaa ggaaagacaa gtatgaatat tccaatttcc atcatcatgg
    19861 gctctagttc cgactggaaa accatgaaaa aagcagccga tgtgctggac aaatttggcg
    19921 tagcctatga aaagaaagtg gtctctgccc accgcacccc agacctcgtg ttccgtcatg
    19981 ccgaagaggc gcgtggtcgt ggtattaagg ttattatcgc gggagcgggt ggtgcggctc
    20041 atttgccagg tatggtagca gccaagacca ccctgcctgt tatcggtgtt cctgtccaat
    20101 cccgtgccct cagcggtgtg gattcgctct attctatcgt gcaaatgcca ggcggcgtac
    20161 ctgtggcaac gatggcaatt ggagaagcgg gagcaaccaa tgcggctttg actgccctgc
    20221 gtatcctgtc catcgaagat agcggtatag cagatcaact ggccagcttt gctattgaac
    20281 aaggaaaaat cgcggaggct atgagcgatg aactcaacta aaacaatcgg gattattggt
    20341 ggcggtcaac tgggtcagat gatggccatt tctgctattt acatggggca caaggtcatc
    20401 acgctggatc cagcagcgga ctgcccagct tccaaggtta gcgaggttat cgtcgctccc
    20461 tatcacgatg tggcggccct caaacagctg gcggagcggt gcgatgtcct gacctacgag
    20521 tttgaaaatg tcgatgccga cggactggac gcggtcatca aggacggcca gctccctcag
    20581 gggacagacc tcctccgtat ttcccaaaac tgcatttttg agaaggattt tttggcaaaa
    20641 aaagctggcg tgcaagtcgc cccctacaaa gtcgtcacat ctagcctaga cctagaaggt
    20701 ctggacctca gcaaaaacta cgttttgaag acagctactg ggggctatga tggacatggt
    20761 cagaaggtca ttcgagaagc cgcggacttg gaggcggcta gtcagttagc caactctacc
    20821 gagtgtgttt tggaagagtt tgtgaatttc gacctggaaa tctccgtcct tgtgtctggc
    20881 aatggctccg actacaccgt ctttcctgtg caggaaaata tccaccgcaa taatattctt
    20941 tacaagacca ttgtgcctgc tcgtatttct gatgaacttg ctgaaaaagc caaaactatg
    21001 gccctgcaaa tcgcagacaa actccatttg gcaggcaccc tctgtgttga gatgtttgtg
    21061 gcagggcaag aaattcttgt gaatgaaatc gctcctcgcc cgcacaattc aggacattat
    21121 tcgattgaag cctgtgattt ttcacagttt gacacccata tccgaggcat tttaggggag
    21181 cccctgcccc ctatccgcct gctttctcca gctgttatga tcaatgtcct cggacaagac
    21241 atggaagcag tccaaacctt tcttcaagaa aaccctaccg ctcatgccca cttttatggt
    21301 aaactagaag caaagcacaa tcgcaaaatg ggacacgtga cggtgttgac ggaagacgtt
    21361 gaggtgggat ttagacaaga tgaaattaga taggctaaag gaagtagatg ttagagaaat
    21421 ctggaaacat gagcagtatg atttctcgga gtggctgtct aggacagaga atatcgaatt
    21481 actgagcgat gagataggac tgactcttac cgagattgac aaggaagttt tcgttggctc
    21541 atatagatgc gatttagtgg ctattgatga aacaacggga ataaaagtga ttatagaaaa
    21601 ccaattagaa ttaacggatc atgatcatct tggtaaaatc ataacctacg catcgggctt
    21661 agatgctgag gttgtcattt ggattgtaaa aaaagctaga gaagagcata ggagtgctat
    21721 cgagtggctt aacaataata caacgaaaaa tatatctttt ttcctaattg aaatccatgc
    21781 ttatcaaata ggtagttcaa tgcctgctcc taaatttgtt cttgttgaga aaccgaatga
    21841 ttttacaaag ctagctaaag gaaataatca cggtggtgat ttgagtaaag cacaggcgga
    21901 acgtttgtat ttttggactc aatttaataa tgtagtggca actaaaaata aaccgttcaa
    21961 cgttagaaaa gctactacag accactggta tgatgttgct attggaacaa gtgaggctca
    22021 tatttctatt accctagtca ataaatcaaa aagtgtaggg attgaaatct atataaacaa
    22081 taataaggaa ttatttgata agctatcatc acatgctgat gaaatccacg aagaacttgg
    22141 atttgaaatg gaatggaaga ggttagataa tagaaaggtt tccaggatta tatattatat
    22201 aaagggtctg gattttgaga accatgacaa ttacaacgat ttaattgacg aagtaattaa
    22261 caaagttatc attttgagag atgtagttaa aaagtatatc taaaaaaata aatagatagg
    22321 gaaaaaggaa acacaacatg atagaacgct acacacgccc tgaaatgggc aatatttgga
    22381 gtgaagagaa caagtacaag gcttggttgg aggtggaaat cctcgctgat gaggcttggg
    22441 ctgagttggg tgagattccc aaagaagatg tggccttgat tcgtgagaag gcgacttttg
    22501 acatcgaccg cattttagag attgaagagg aaacccgtca cgatgtggtg gcttttacgc
    22561 gtgcggtttc ggaaagtctt ggtgaggaac gcaagtgggt ccactacggt ctgacttcga
    22621 ccgacgtggt ggatacggct tacggctacc tctataagca ggccaacgac atcatccgtc
    22681 gtgaccttga aaactttacc aacattatcg ccgacaaggc gcgtgagcac aagtacacca
    22741 ttatgatggg gcggacccac ggtgtccatg cggagccaac gaccttcggt ctgaagttgg
    22801 cgacttggta tagcgaaatg aaacgcaata tggagcgttt tgatgtggcg gctaagggcg
    22861 ttgaggctgg taaaatttca ggagcggttg gaaactttgc caacattcct ccatttgtgg
    22921 aagagtatgt ttgtggtaaa ttgggcattc gtccgcagga aatttctacc caggtcctac
    22981 ctcgtgacct ccacgcagaa tatttctcag ccctagcctt gattgcaacg tctattgagc
    23041 gtatggcgac agaaatccgt ggactacaaa aatctgaaca acgtgaagtc gaagagtatt
    23101 tcgccaaagg ccaaaaaggc agctctgcta tgccccataa acgcaacccg atcggctctg
    23161 aaaatatgac cggtctggct cgtgtggtgc gtggtcactt ggtgacggct tttgagaatg
    23221 tggctctctg gcacgaacgt gatatttccc actcgtcagc ggagcggatt atcacgccgg
    23281 atacgactat cctcatcaat tatatgctca accgttttgg caatatcgtt aagaacttga
    23341 cggtcttccc tgaaaatatg aaacgcaata tggagtcaac ctttggcttg atttacagcc
    23401 agcgtgtcat gctcagtttg attgaaaaag gcatgacccg tgaggaagcc tatgatttgg
    23461 tgcaaccaaa aactgcgcag tcatgggata atcaagtgga cttcaagccc cttctcgaag
    23521 cggatgagcg tgtgactgcc aaacttagcc aggaagaaat cgatgaactc ttcaacccag
    23581 actactatgc taagcgggtt gatgacatct ttgaacgact tggattataa aaaatcaaaa
    23641 ttctttggat tttccagaga atttttttat ttacttgcga ataaatagtt gaaggggaaa
    23701 acaagctttc tccgctatac aatcattcgt aagtaaaaat aaattatttt ttgcaaaagt
    23761 ttacgaatag ataagtgtag gggaagccag ctcacaactt attcaaaa
//
LOCUS       DAZQCJ010000023        23093 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000023 DAZQCJ010000000
VERSION     DAZQCJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23093
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.023"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..348)
                     /locus_tag="ACHU66_000824"
     CDS             complement(<1..348)
                     /locus_tag="ACHU66_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PlySs2 family phage lysin"
                     /protein_id="HFR3389122.1"
                     /translation="MTTVNEALNNVRAQVGSGVSVGNGECYALASWYERMISPDATVG
                     LGAGVGWVSGATGDTISAKNIGSSYNWQANGWTVSTSGPFQAGQIVTLGATSGNPYGH
                     VVIVEAVDGDRLTI"
     gene            complement(332..682)
                     /locus_tag="ACHU66_000825"
     CDS             complement(332..682)
                     /locus_tag="ACHU66_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR3389123.1"
                     /translation="MNQLTEIIIGSATGILAIVAGMIVHEVKKYLIAKGGKRAVEITE
                     ILARNAVNAVEQIAKLDQDKHVDKLDMAKRRVTGQLAKYNIYMTDTQLETFIESAVKQ
                     MNDAWKGEAYDNSK"
     gene            complement(685..984)
                     /locus_tag="ACHU66_000826"
     CDS             complement(685..984)
                     /locus_tag="ACHU66_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389124.1"
                     /translation="MPIEHAERIAQSQVAWAILFILLFGFVIRYLIKTSDKREAKLMD
                     FHEQAKEESNKREDRLMNHLEKTTAEMGAMAREIGGLKGEVSLMSDRIEKIEKGE"
     gene            complement(988..1332)
                     /locus_tag="ACHU66_000827"
     CDS             complement(988..1332)
                     /locus_tag="ACHU66_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389125.1"
                     /translation="MTETTNNTLLNLEETTQPFDLATALQYMKENGEFIRCKNATNDF
                     YMYRDVQKRPAIVNGRRKFVDVETIWAFNQWGGTAATINIADMLNEEYWIMKFDENGN
                     PDWTDPTAGAEA"
     gene            complement(1335..1553)
                     /locus_tag="ACHU66_000828"
     CDS             complement(1335..1553)
                     /locus_tag="ACHU66_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389126.1"
                     /translation="MNQEQITQALRLTNNDLVTKLSEEMTTKNLLAVQLTEAQQTIAN
                     LRAEITDLTQQLDEATKPEEIIDQEEGE"
     gene            complement(1562..6832)
                     /locus_tag="ACHU66_000829"
     CDS             complement(1562..6832)
                     /locus_tag="ACHU66_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp58-like family protein"
                     /protein_id="HFR3389127.1"
                     /translation="MVHTVTFNQAMLAKDRVFAIRAGAYTSSDIKEASFNYGYISGDT
                     LKPGGTVAGSAKLTFTSIITSFNKLDKVYPEIGLKVGDSFEWVAMGEYFVNDINIDRN
                     RNTTELDLMDGMFKLNQPYISDLTYPAQIRDVIREICVKTGVELETDDLGFRAIQHHI
                     QSKADKKDITFREVLSQAIQLLGFSAFFNRKGKLEIRGLTESNITITADNYFLHGLTK
                     SELMYQIAGITCKKDKETLTVGLRTGRSLELENSFMIQNILDDLYYDLKEIKYYPFSL
                     DWQGHLKLDVGQWITLKTNKNETFKVPVLSQSFNFKGGLKSKISADSKAGNDTQYSYK
                     GFLGKRIEQMSTEIEAEVQQQLEYKDKEFDEKINKAKSEINDGIEQAQAEAERYADAI
                     KQEIDTEIAQVNQSMQSQEQEHDREVANILSKTQSVESLANQAKADAANAIARANQVK
                     TEAIADARAQVATVNQALNTAKTDLQNQVNAIDAKAVQAQRDITQAKYDLQSQASQLI
                     AQATKQVELTKLTTETKKLADGTLTSLNELTKTVDKTTGDLTSVTNRTKVVEDSLAGV
                     KTNYTQLNQTVNAQTGQIDSINRKTADLQSGIDGVTERFKSLKIGSRNYFKNSKSRKY
                     YITSLTTQDVRTYIDEEFWQNDTRFVKDYVRVSFDIAFNPALQSDFTTTVHFSATPWY
                     GAGITFKGGTTALQHFDLKFNLSDATKAYKTDNVFIRLTNTIPLNTAVSLENFNLYLS
                     AVIEDYSQSNADFESKIAEYKRTADQNYAGLQSTVSTLDGKVTQNKTEANQTATQLSN
                     RLTSLETYKDGESTRAQSYFEASKTETAKQLTAERTAIATNYVAKSTYTEDVRGTTQK
                     LNEIKSTADTAKQNLATYQNTVDRKLEELTSSTQTLDGKINTASAKVDTVAGQIRTEI
                     GTVEAKIPTEAGGRNYILKSQAEISSTGRWVSKPFNLSSDLLSNLSKIKTVTISCDVE
                     GINVSALNSRKRYGLACSVEINGVVNYWEVWQTQDTTKKRISQTFTVPEGKVITKFHS
                     PTLWIQAAGDIKVSNPKIEFGRVPTDHTLAPEDFANELSSVKTTITQTASGVEQLSTS
                     LATTDNKVTTAEAKIRQLISDVSSKVSQTDYNTLTGRVDDAETAITQNATEISKRLTK
                     TQVDKAITDKGFQTASQVDTAIAGKGYQTKSDVDNNITGRGYITSSALQPYALSTTVQ
                     NLVKETAGSFERQITETRGLIPSNAGTRNLLKGTKDLSGNDAKSFNTSDKYLDFNIAR
                     SRPTTGYSDTFSAYTTIPVTANDYIISFYAKSDVDGATLYCHFYNPNTTTKAESSTGY
                     KSGSSDGLARVQVTTEWQRYWVKWSQSKTDTVKKVIIGRNNSADGIKIIEVAGVALYE
                     GALNKGHFDAPEDLATVTALHNVNDTVDSHTRTIGAVGTTGSILDNVSKVTQTAAGLV
                     QEVSGTNGLKTQVSTLAGSYAIKSLTKAGDVLGQINLNRDGSIKLDGSLVQITGKTYI
                     QDGVISAAKIGDLDAGKIKTGTLDAARIKANSIDGSKLVFDQAFVNKMTANEALFKQL
                     FAQSAFITSVQAVAVSAKQIVGGIAKALNGGMDVNFDESKINFYTNVAAIRRIYTGHP
                     TQFIKFETEGNYSRTIIGSNRNGGEVFNSATFAGIVVENTNNINTEDNVRIYGDNTLL
                     RHAQGDVGWNINSVTQRIVPANMNAESEIWSKHFVAPDKNSKPVRLDTAVAALWDIWN
                     HIIYNNFEFNAALRTHIKARRDNWKFELNL"
     gene            complement(6834..7184)
                     /locus_tag="ACHU66_000830"
     CDS             complement(6834..7184)
                     /locus_tag="ACHU66_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6711 family protein"
                     /protein_id="HFR3389128.1"
                     /translation="MSRLIINGVTVVPPKSFQVAINDVDGETGRNANGDMVRDRITTK
                     RKLECDWGMLTQAEMAQIQNAVQPVFFEVSYPDPILGQTSKTFYVGDRTAPAYSFDEK
                     LKPWSGLKFSLIER"
     gene            complement(7194..10331)
                     /locus_tag="ACHU66_000831"
     CDS             complement(7194..10331)
                     /locus_tag="ACHU66_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389129.1"
                     /translation="MATTLEELRVIVEGEIAPFQKKMKQLESQMKQTQNKIENKTKGL
                     RERVGQQAGGMAAALGKLAKITALAYLGKKMLDLGMYSTQMALEVSASVNQIKRQMGE
                     SSQAFLKWIDNNANAMNMSVGEATKYGAVYSNLFSNFIKDSDKLSAYTGKMLQTSAVI
                     AQGSGRTMTDVMERIRSGLLGNTEAIEDLGINVNVAMIESTNAFKRFANGQSWQQLDY
                     NTQQQIRLMAILEQATAKYGNTLQQSVNGRISLFKSLLSDAALNIGNAMLPIINAMMP
                     VLNSFAMVLKNVTAKLAEFIGLMFNKKANVKNSAIGNLAQGAQNANDAVGGLGDAMDG
                     VDDASGGTADNLDDTAKSAKKAAKELLGLAGFDEITTLNLNKDDSDGAGSGSGGGKGG
                     KNGKGGSSGNGTDILPEIELTDMDNQFKSIFDGWDKTLQPLFDYLSKLKDLFKDGFNM
                     SFRADSLDRFKNALVGIWQSLKDIFADGTVLQAAARFGEKLSYALGQGTGAVANVVMG
                     IAVFIAESLDKSSKETKLDIKNWLIRQFEIKGDTITSIGNLAQAIGQIFYDTITSVAA
                     TDIGSAIISSLIYIRMGIDDITGKIERDFWAFWERLAVDNQAGITTAFIGLLSAVEPI
                     FASIKDLFKNTFISLNATYDEHLKPFFDSFIEGFSSIFGTLIDSWNNDVQPVLDSIGQ
                     LFSDMFDNHIQPFVDNFLYAFGQVVDLLKVVWEEGFLPLFDWIAANILPVLVPIFQTL
                     AEWFVQAWNVVFDVLGAVLKILGGIIEFLVGVFTGDWEKAWDGIVQVAKGMWDLLSSI
                     FMFVWNVILSFLKGVWNTIVAILQAGWDAIVRIFQGLGKWFGDRWKDVENIFSNVGRW
                     FGQKFSEAWNGITNAFSNVVGFFRGIYDNIVSWFSNIGGAVATAVSGAFRYAMNGVFA
                     TIENAVNGFIGMINGVIGLINNIPGVSLGSIGYVNLPRLARGGIVDSPTVAMIGEAGK
                     EVVMPLENTGFLQTMGRVVGGAVVNALGGGLPQSSGLPSGDIVINIGSREFGRFAIDE
                     INKAQAQAGELLLNI"
     gene            complement(10356..10715)
                     /locus_tag="ACHU66_000832"
     CDS             complement(10356..10715)
                     /locus_tag="ACHU66_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389130.1"
                     /translation="MINGFYPTALDAGVDPFSFWEYTLLELKELVESYNRQQFQRQKE
                     IASHNFVQSQMIARFVSMMFQEKGEAPDIWDFYPTLFEEDRRQIEQARIERDLIIHRE
                     RMRAFAERMRGRFKTSE"
     gene            complement(10742..11116)
                     /locus_tag="ACHU66_000833"
     CDS             complement(10742..11116)
                     /locus_tag="ACHU66_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6096 family protein"
                     /protein_id="HFR3389131.1"
                     /translation="MSTRKPYITWTVKGTDYKLRLSTRQACEVEEKLGVNLLKIFMPQ
                     PGEQFNLPPLKVMLLVVQGALQKFHHGIKLDDVYDLFDDYIDEGYGQTELMSDIIVPL
                     FEVSGFIPRNKEKEEPTLTVVE"
     gene            complement(11127..11543)
                     /locus_tag="ACHU66_000834"
     CDS             complement(11127..11543)
                     /locus_tag="ACHU66_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR3389132.1"
                     /translation="MLANGIKLKMSETKGSSYTVIEGLKEVPELGIDPEKVENTTLAD
                     TIKQYEFGIGDAGELEYKFKYENSKTTSSYRTLRKLADAKAVRHFEQEYPDGTKVHFS
                     AQIAVKLGGGGVNSAIEFTLKLALQSDLEFTDPVAL"
     gene            complement(11547..11915)
                     /locus_tag="ACHU66_000835"
     CDS             complement(11547..11915)
                     /locus_tag="ACHU66_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389133.1"
                     /translation="MINIKPIIYKKLKEVADNVTDTYPQDWENFPVIIYLEEENKPYE
                     ITDDTEQMSYLRYKVDIFHNDSTSELAVAIDAIFASLGLKRTSSVDTPDPTHLRHKVM
                     RFEGILDLNSRIVYQYRMEG"
     gene            complement(11912..12424)
                     /locus_tag="ACHU66_000836"
     CDS             complement(11912..12424)
                     /locus_tag="ACHU66_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFR3389134.1"
                     /translation="MSRLIGADRLISKLKRLSSQRQTEIIAKAVHNAAKNVVQADAKL
                     RAPVNDGDLRAGIKVRMSKPGNPRAEVVSTSDHGGFVEFGTGPKGATNHAGISPEVSV
                     SYRSTPWYVHESQIDAGPYRFPKIGEFYKMFGQVAQPYLYPALKDNEERVTKNINRYV
                     KRKLIEEVSK"
     gene            complement(12692..13021)
                     /locus_tag="ACHU66_000837"
     CDS             complement(12692..13021)
                     /locus_tag="ACHU66_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFR3389135.1"
                     /translation="MLEDLKTLTGESDDKILSSLLLRAKNIILTETNRSQLTPALEGL
                     QLEVALELFNRQGSEGETSRSEGGVSVSYKDGLSDTILNGIRSHRLARVAGRAFEAKP
                     TEAVSDS"
     gene            complement(13031..13219)
                     /locus_tag="ACHU66_000838"
     CDS             complement(13031..13219)
                     /locus_tag="ACHU66_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389136.1"
                     /translation="MRFKVLKEFTDDELGFVHRVNDIIELTKERHKQMKKNAKLQDVN
                     LADYIEEIKTKGAEAPAK"
     gene            complement(13230..14246)
                     /locus_tag="ACHU66_000839"
     CDS             complement(13230..14246)
                     /locus_tag="ACHU66_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HFR3389137.1"
                     /translation="MPFENINTATSREKFLGIIEKVVAKKSYSAPLLLSNDAVEMNGR
                     SFTVTKSDTTELKDYKRNADNEFDHAQTEERTYTLDQEKYWGRFVDRLDERDSNGEVN
                     VNYVVARQAAEVVAPYLDHLRFDALLGNVSDNVVPANTKGANNSYQAVLDVSEKLDEL
                     DVVENRLLFVTPAFYKAIKSEIVNLPQGDTNQTVLYKGYVGQLDTFTVYKVPSKYLKG
                     VQAVATIGGVVVSPIQVDETKYNNNIPGRFGELVEQLLYTGAFVFDFDQKYIISIATS
                     KPEAKPSAQGELNIRAEQWVSGATYEAGARVQNEGKLFEATKKVNSSSTAPGSDSANW
                     KEIV"
     gene            complement(14264..14785)
                     /locus_tag="ACHU66_000840"
     CDS             complement(14264..14785)
                     /locus_tag="ACHU66_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFR3389138.1"
                     /translation="MNEETQTVEAVEDDKQVAAEPEQVTTDPKDEKKYTDADVDAIID
                     KEFAKWKAEQEKAESEAKKLAKMNAEDKQKYQLDKREQDLADREAEITRRELTAEAKT
                     ILSERGLPIELVDVVNLADADSVRDSIDAIQKTWEAAVLKGVADKTKGSAPMKKAPQE
                     QPTVEKWERDFLK"
     gene            complement(14949..15239)
                     /locus_tag="ACHU66_000841"
     CDS             complement(14949..15239)
                     /locus_tag="ACHU66_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389139.1"
                     /translation="MRYRKKPVEIEAVQWNGRNFEDVYSLCGRSQLNYDEETNTLYIL
                     TLEGVMRADEGCYIIKGIHGEIYPCKEDIFNDTYEAIGPQNIVGFRSVLDSM"
     gene            complement(15251..15487)
                     /locus_tag="ACHU66_000842"
     CDS             complement(15251..15487)
                     /locus_tag="ACHU66_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389140.1"
                     /translation="MNKRIKKKYRLIQALEQWLLAISTTIDIIVNGQNRLYNRVAELE
                     NIIERNAQATNSRFDYLEKKVANKLSKKSWFSRK"
     gene            complement(15701..15901)
                     /locus_tag="ACHU66_000843"
     CDS             complement(15701..15901)
                     /locus_tag="ACHU66_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HFR3389141.1"
                     /translation="MKTTNEIFLIDGEEYIRCPVCGRNVMLFDVCECNWENTGETNID
                     GGPNKMTLEEAKKAYAEGRPII"
     gene            complement(15870..17381)
                     /locus_tag="ACHU66_000844"
     CDS             complement(15870..17381)
                     /locus_tag="ACHU66_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFR3389142.1"
                     /translation="MDNQKYWEKRKAQRMVQEMDKAEQTAKQLDEIHKLASRHITSKI
                     DQIFESYRRDHGLTEDEARRVLDNVKNLSDIRELKLALQNTTDSEEIRQLLILLDLAP
                     YASRIERYEALQREVDNLPTRLYKAENEASRAFYDEFIPDAYYHSVFDLQQQSGVAFA
                     FNRIDPEEIRAIQQTPWLGANYSERIWGNTQALASELQKQLAVSLLTGRSAHETAEVI
                     EAQFGKGSQNARRLIRTETSYFHAEMEAKAYEEADVEYYRFLATLDLRTSSICREHDG
                     KIYKVSERITGKNYPPMHPWCRSDTIASDDSEWLAKATRSARDPVTGKTIQVPANMTY
                     KDWYEKYVKPKYKADNLDIWKIERANNQYEKYKSILGDKAPKSLEDYIDLKYNDKEGY
                     GQLQDQARWIKAKFPSEKSFNGHFEKHGHEFPSLTKEEYRKLASELIASPTDENILGY
                     ETEGGRRVRYDKAENIIVIGRRNKNNQARLNTMLKPDEGEKYYYENYKRDFPD"
     gene            complement(17290..18738)
                     /locus_tag="ACHU66_000845"
     CDS             complement(17290..18738)
                     /locus_tag="ACHU66_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR3389143.1"
                     /translation="MEEVLVYSRSLYNEQNLDKDIIYKLIVKHDQTSSKLKKLKDYYL
                     GKHAIEKHTRRSNLPNFKTVANHAKDIADTATGYFMGNAIRYPKTDDMDIEDLLKTFD
                     SADVDSTDLDNALNMAIYGRAYEYIYVKEDENELVTRSLEPENTFIVYDDSIEQKPLF
                     AVYYYQTKDDVTEETYYRAQVLTENLQYSMSLREQKKESEEGVPHNLGALPIIEYRNN
                     RYMVGDYEQQISLIDAYNSLMGNRVNDKEQAIDSILVLYGAALADTPEEAKEAMEILR
                     EEGLLELPKDASAEFLKNVLDEATVEVLRKALKEDIYTFSHVPNLSDENFAGNTSGVA
                     MEFKLLGLEMITKTKERYYIKSLHKRIQIFANYYNWSQIYENAKAIIPQFSRGLPKNL
                     LELSQIISNLKDKVSLRQLISLLPFVEDPDAEIEALEKEKEAAQEEPAFSQNLPYEES
                     VTDGQSEVLGEAESSANGSRNGQGRANRKTTR"
     gene            complement(18751..20172)
                     /gene="terL"
                     /locus_tag="ACHU66_000846"
     CDS             complement(18751..20172)
                     /gene="terL"
                     /locus_tag="ACHU66_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFR3389144.1"
                     /translation="MMDRAAIRQQARFELARRDFFYYCHLMASDFYKPSRRYLVELCN
                     DLQGFLSDNEHNVLVINEPPRHGKSRTAGMFVQWLLGNDNDKKIMTGSYNETLSTVFS
                     KNVRNAIQETKADEDVVVFNDIFPDTHIKYGDAAMNLWSLEGGYNNYLATSPTGTATG
                     FGADIIIVDDLIKNAEEANNATVLEKHWEWFTNTMLSRLEEGGKIIIIMTRWHSQDLA
                     GKALIELPKSDYKVKHISMKAYDEATDTMLCDEVLSKQAYLQKTKTMGADIASANYQQ
                     EPIDIKGRLYSGFKTYVDKPTFKRISAYTDTADTGKDYLASYIYGVTMDNEAYILDVV
                     FTKEPMEVTEPLLAQKLAEWQVNTCDIESNNGGRGFARNVERLTQDSYQNRYTVFNWF
                     HQSQNKQARILTNTTWAIEHIYFPENWRHRWSELYQNLMSYQREGKNAHDDAADALTG
                     VVEAINDKIRTKAKVKRKSLYGL"
     gene            complement(20162..20557)
                     /locus_tag="ACHU66_000847"
     CDS             complement(20162..20557)
                     /locus_tag="ACHU66_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389145.1"
                     /translation="MAKTKRGRPTKMTQGTLRKLEELFVRGLSDEEACLLADIGTTTL
                     YDYCKENPEFSERKELLKQRVKTRAKLNISKAIEDGDIDLSKWYLERRDNDFKTKQAV
                     THDGEVNINQTNPFADLTTDELRKLIDDG"
     gene            complement(20620..21024)
                     /locus_tag="ACHU66_000848"
                     /pseudo
     CDS             complement(20620..21024)
                     /locus_tag="ACHU66_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693341.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21017..22282)
                     /locus_tag="ACHU66_000849"
     CDS             complement(21017..22282)
                     /locus_tag="ACHU66_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR3389146.1"
                     /translation="MSYKGMSYLRKKLAMYQPGVKKRYRFYAMTDRDNTRSFIIPDSV
                     KSMYESVIGWTAHSVDALADRIIFREFTNDDFEATEIFAANNPDIFFDTAIQSALIAS
                     CCFIYLVPNQNGLPKMQVIEASKATGVIDTTTFLLTEGYAILEVDENDNPLLEAYFTK
                     DVTWYYPKGANPYSISNPTGQPLLVPIIHRPDAVRPFGRSRITRAGMYNQLAAKRTLE
                     RAEVTAEFYSFPQKYVLGTSQDADPMEKWKTTVSSLLEITKDDDGDSPNVGQFNTASM
                     APFIDQLRMYASLFAGGSGLTMDDLGFPSDNPSSVEAIKAAHENLRAAGRKAQRSIAS
                     GLLNVAYVAVCLRDRYQYTREQFLNTKIKWEPLFEADANMLTLIGDGAIKLNQALPGY
                     INAETIRDLTGIEGDMSATPKVAEVLANE"
     gene            complement(22279..22641)
                     /locus_tag="ACHU66_000850"
     CDS             complement(22279..22641)
                     /locus_tag="ACHU66_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389147.1"
                     /translation="MGKIKCEICGKSFVRKTSRSKYCSDKCRSDGNREKKRLQMKRLR
                     AEAKKEKDDKSNRNIKLTKKRKKKKNLLKFYQDFKTKILANEAEFGFTSRTVIEGVEI
                     HEPDFEEQVINKIKEQSK"
     gene            complement(22720..23067)
                     /locus_tag="ACHU66_000851"
     CDS             complement(22720..23067)
                     /locus_tag="ACHU66_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR3389148.1"
                     /translation="MRADKSGTHRVAFEKNKKRILKTATHCGICGQLVNKKLKYPNPM
                     CAVIDHVVPLAKGGHPSLIENLQLAHMSCNRIKSDKLFADNSKAEPKTVGNRNLPQSQ
                     DWSKFICAKSEGG"
BASE COUNT         5962 a         5421 c         4270 g         7440 t
ORIGIN      
        1 aatggtcaat cggtcaccat ccaccgcttc tacaatcacc acatgcccat aggggttgcc
       61 tgatgttgca cctaatgtca caatctgacc tgcttgaaat ggcccagatg tggatacggt
      121 ccagccgtta gcttgccagt tatatgatga gccaatattc ttggctgaaa tcgtatcacc
      181 agtggcacca ctgacccaac caacaccagc cccaaggccg actgttgcat ccgggctaat
      241 catgcgctca taccaactgg ccaaagcata gcattcacca ttgccaacag acacaccgga
      301 cccaacctga gctcttacat tatttaatgc ttcatttact gttgtcatag gcttctcctt
      361 tccacgcatc attcatctgc ttgactgcgc tctcgataaa ggtctctaac tgtgtatcag
      421 tcatatagat gttgtattta gcaagctgac ctgttacgcg acgtttagcc atgtctagct
      481 tgtctacatg cttgtcctgg tctagcttgg cgatttgttc aactgcatta acagcgttcc
      541 gtgccagaat ctctgtaatt tcgaccgctc gcttaccacc cttggcaatc agatactttt
      601 tgacctcatg gacaatcata ccagcaacga tagctaatat accagtagca gaccctataa
      661 taatttctgt aagttgattc atacttattc tcctttttcg attttttcaa tacggtcact
      721 cattaatgac acttcacctt ttaagccacc gatttcacga gccatggctc ccatttctgc
      781 ggtagtcttt tcaagatgat tcatcaagcg gtcctcccgc ttgttacttt cttccttggc
      841 ctgctcatgg aaatccatga gcttagcttc tcgcttgtct gacgtcttaa tcagataacg
      901 gatgacgaat ccgaataata aaataaacaa aatagcccaa gcaacttgac tttgggctat
      961 tctttctgcg tgttcgattg gcataggcta cgcctccgct cctgctgttg ggtctgtcca
     1021 atctggattt ccgttttcat caaatttcat gatccaatac tcttcgttga gcatgtcagc
     1081 aatattaatt gtcgcagcgg taccgcccca ctgattaaag gcccagatag tttccacatc
     1141 cacaaatttg cgacgaccat tcacaattgc aggacgtttt tgcacatcac ggtacatata
     1201 aaaatcattt gtcgcattct tgcagcgaat gaattccccg ttttccttca tatactgcaa
     1261 tgcagtcgca aggtcaaatg gttgtgttgt ttcttccaaa ttaagcaatg tgttgttagt
     1321 agtttctgtc atgattattc tccttcttct tggtcaatga tttcctctgg tttagtagct
     1381 tcatccagtt gctgagttaa gtcagtaatt tctgcccgca gattagcgat ggtctgctgt
     1441 gcctcagtta gttgcacagc gagcaagttt ttcgtcgtca tctcctctga cagttttgtc
     1501 acgaggtcgt tattagtcaa gcgtagcgct tgagtgattt gttcttgatt catgattcct
     1561 cctataaatt taattcaaat ttccagttgt cccgtctagc ttttatgtgt gtgcgaagcg
     1621 ctgcgttgaa ctcaaaattg ttgtagatga tgtgattcca aatgtcccat aatgctgcca
     1681 ctgctgtatc caatcggaca ggctttgaat ttttatccgg agccacaaag tgcttagacc
     1741 aaatttcgga ctctgcgttc atgttagctg ggactatacg ttgagtgaca gaattgatat
     1801 tccaaccgac atcgccttgt gcatgtctta atagcgtgtt atctccataa atccgcacat
     1861 tatcttctgt gtttatgttg tttgtgtttt ctacaacaat ccctgcaaat gttgccgaat
     1921 tgaaaacttc tcctccgttt cggttgctcc cgatgattgt tcgtgagtaa ttcccttcag
     1981 tttcgaattt tataaattga gtagggtgtc cagtatagat acgtcttatt gcagctacgt
     2041 ttgtgtaaaa gttgatttta ctttcgtcga aattgacatc cataccaccg ttgagagcct
     2101 tagcaatacc accaacaatc tgcttagctg acacagccac tgcctggacg cttgtgataa
     2161 atgcactttg agcaaacaac tgcttaaaca aggcttcgtt tgctgtcatc ttgttgacaa
     2221 aagcttggtc gaatacaagc ttactaccgt cgatggaatt agctttgatc cgtgcagcat
     2281 ccagcgtacc agtcttgatt ttaccagcat ccaaatcgcc aattttagca gcgctgatta
     2341 caccatcttg gatatatgtc ttgccggtaa tctgtacgag actgccgtca agcttaatag
     2401 agccatctct gtttagatta atctggccca acacatcgcc agcctttgtc aggcttttga
     2461 tcgcataaga ccctgcaagt gtgctgacct gggtcttaag cccgttagta ccagacacct
     2521 cctggaccaa gcctgctgca gtctgcgtaa ccttgctgac attatccaaa atacttcctg
     2581 tcgtaccgac agcgccgatg gtacgagtgt gactgtcgac tgtgtcgtta acgttgtgaa
     2641 gagctgtgac ggtggcgagg tcttctggcg catcaaaatg gcctttattt aatgcacctt
     2701 cgtacaacgc gacaccagca acttctatga ttttgatacc gtctgcgcta ttgtttcgac
     2761 caataatcac tttttttact gtgtcagttt tgctttgaga ccacttgacc cagtaacgtt
     2821 gccactcagt tgttacctgt acccgcgcta atccgtccga actaccactt ttataacctg
     2881 tgcttgattc ggcttttgta gtcgtgtttg gattgtaaaa atggcaataa agcgttgcgc
     2941 catcaacatc agatttagcg taaaagctaa tgatataatc atttgccgtt acgggtattg
     3001 tcgtatacgc gctaaacgtg tcagaatatc cagttgtagg tctcgagcgt gcgatgttaa
     3061 aatcaaggta tttatctgat gtgttaaagc ttttagcatc attaccgctt aaatcttttg
     3121 tcccttttag tagatttcgg gttccagcgt tgctaggtat cagacctctt gtttctgtaa
     3181 tctgacgctc aaagctacca gctgtctctt tcacaagatt ttgtacggtc gtagatagcg
     3241 cataaggttg cagagcacta ctagtaatgt atccacgacc tgtgatgttg ttgtcaacat
     3301 cagacttggt ctggtaacct ttaccagcga ttgcagtgtc tacttgcgat gcagtctgaa
     3361 accctttatc tgtgattgct ttatcaactt gcgtctttgt caatcgcttg ctaatctcgg
     3421 tcgcattttg agtaatagct gtttcagcat catccacacg gccggtcagc gtattgtagt
     3481 ccgtttgcga tactttgctt gacacatcgc taattaactg tcggatttta gcctcagcag
     3541 tcgtgacttt gttatcagtc gtagctaagc tagttgataa ctgctctaca ccggatgcag
     3601 tctgagtgat tgtggttttg actgatgata attcattggc aaaatcttca ggcgccaatg
     3661 tgtgatctgt aggtaccctt ccaaactcga ttttgggatt ggaaactttt atatctccgg
     3721 ctgcctgtat ccataatgtc ggcgagtgaa atttggtaat tactttccct tcaggaacag
     3781 taaacgtctg gctaattcgc ttcttcgtgg tatcttgcgt ttgccaaact tcccaataat
     3841 tcactacacc gtttatctca actgaacaag ctaaaccata cctttttcgt gaatttaaag
     3901 cggaaacatt gataccctcg acatcacaag atattgtgac agttttgatt ttcgacaaat
     3961 tagatagtaa gtcactcgac aaattaaatg gtttgcttac ccacctacct gtgctactga
     4021 tttcggcttg agatttcaaa atgtaatttc ttccgcctgc ctcagtcgga atcttcgcct
     4081 caacagtgcc aatctcagtc cgtatctgac cggctacagt atcaaccttt gcgctcgctg
     4141 tattgatttt accgtcaagc gtttgagtac ttgatgtcag ttcttccaac ttacggtcaa
     4201 ccgtgttttg atacgttgct aaattttgct ttgcagtgtc agcggttgac ttaatctcgt
     4261 taagtttttg ggtcgttcca cggacatcct ctgtgtatgt tgacttagcc acataattag
     4321 tagcaattgc agtacgctcg gctgttaact gtttggccgt ctctgtcttt gatgcctcaa
     4381 aatacgactg agcgcgagtt gattcgccgt ccttgtatgt ttcaaggctt gttaatctgt
     4441 tagataactg cgtagctgtc tgattggctt cggtcttatt ctgagtgacc ttaccatcta
     4501 acgtgctaac ggttgattgt aagccagcgt agttttggtc ggcagtccgc ttgtactcgg
     4561 cgattttgga ctcaaaatca gcgttgcttt gagagtaatc ctcgattact gcggataaat
     4621 atagattaaa attttcgaga cttacagcgg tattgagggg gattgtattt gtgagacgga
     4681 tgaatacatt gtctgtctta taagcttttg ttgcgtcgct caaattaaac tttaagtcaa
     4741 aatgttgtaa cgcagttgtg ccacctttaa acgtgatacc tgctccatac cacggagttg
     4801 ccgaaaaatg gacagttgtt gtaaagtctg attgtaaggc tggattaaac gctatatcaa
     4861 aagacaccct tacataatct ttgacaaatc gtgtatcatt ttgccaaaac tcctcatcaa
     4921 tataagtcct cacatcttga gttgttaaac tagtaatgta gtatttacga gattttgagt
     4981 ttttaaaata gttacgacta ccaattttta aactcttaaa ccgctctgta acgccatcaa
     5041 taccactttg taaatcagca gtcttacgat tgatactatc aatttgacca gtctgggcat
     5101 ttaccgtctg attgagttga gtgtaattag ttttaacacc agctagacta tcctcaacga
     5161 ctttagtacg attggtcacg ctcgtaaggt cgccagtcgt tttgtcgaca gttttagtca
     5221 gctcgtttaa gctcgttagc gtgccatctg caagcttttt cgtttcggtg gtaagcttgg
     5281 tcaactcaac ttgtttagtc gcttgtgcga taagctgtga ggcctgactt tgtaaatcat
     5341 acttagcttg tgtgatatct cgttgtgctt gcacagcctt agcatcaatc gcgttaactt
     5401 gattttgcaa atcagtctta gcagtattta acgcttgatt aacggtcgca acctgcgctc
     5461 ttgcatctgc gatagcttcg gtcttgacct ggttagctct agcgatggcg tttgccgcat
     5521 ctgccttggc ctggttggca agcgactcga cagactgggt tttagacagg atgttcgcaa
     5581 cctctctgtc gtgttcctgt tcctgggatt gcatggattg gttgacttgg gcgatttcag
     5641 tatcaatttc ctgtttaata gcgtcagcat accgctcagc ctcagcttgg gcttgctcga
     5701 taccgtcatt gatttcggat ttggctttgt taattttttc atcgaattcc ttatccttat
     5761 attccagttg ctgttgaacc tctgcttcga tctcagtaga catttgctcg atgcgcttgc
     5821 ctaaaaatcc cttataagaa tactgagtat cattaccagc tttgctgtct gcactaattt
     5881 tggattttag accgcccttg aaattaaaag attgactcag tacagggact ttaaaagtct
     5941 cgtttttgtt tgtttttaac gtaatccatt gcccgacatc cagttttagg tgtccttgcc
     6001 aatcaagaga aaatggataa tacttgattt ctttcaaatc ataatacaaa tcgtctaaga
     6061 tattctgtat catgaagcta ttttctagct ctaaagagcg accagttcgc aatccaactg
     6121 ttaacgtctc tttgtctttc ttgcaagtga tacctgcaat ctggtacata agttcgcttt
     6181 tagtcaggcc gtgcaaaaaa taattatcag cagtaattgt gatatttgat tcagtcaacc
     6241 cacgaatttc caatttgcct tttctgttaa aaaaagcaga aaagccaagc aattgaatcg
     6301 cttgacttag tacttctcta aaagtaatgt cctttttatc cgcttttgat tggatatgat
     6361 gctgaatcgc tcggaaacct aaatcatctg tttctaactc tactcctgtc tttacacaaa
     6421 tttcgcgaat gacatctcta atctgtgccg ggtaagtcag gtcagaaata taaggttgat
     6481 tgagcttgaa catcccatcc atcagatcta attctgtggt attcctgttg cggtcgatgt
     6541 taatatcgtt gacaaaatac tcacccattg caacccactc gaaggaatcg ccaactttta
     6601 gtcctatctc tggataaact ttatccaatt tgttaaagct agtgatgata gatgtaaagg
     6661 tcaatttagc cgaaccagca actgttccgc caggttttaa agtatcgccg ctgatatatc
     6721 cataattgaa actagcttct ttgatgtcgc tagaagtata ggcgcctgca cgaatagcaa
     6781 acaccctatc tttagctaac atagcttggt taaatgttac cgtgtgaacc accttacctc
     6841 tctattaaac taaattttaa accgctccat ggtttgagtt tttcatcaaa tgaatatgct
     6901 ggtgctgttc tgtcaccaac atagaatgtt ttagaggtct gccctaagat agggtccgga
     6961 taggatactt caaagaaaac aggctgaaca gcattctgta tctgagccat ctcagcctgt
     7021 gtcaacatcc cccaatcaca ttccaatttg cgcttggttg taatcctatc cctgaccatg
     7081 tctccgttag catttcgacc tgtctcgcca tctacatcat tgatagcgac ttgaaaagat
     7141 ttaggaggta ctactgtaac tccattgata atcaatcgac tcatgtttcc ctcctaaatg
     7201 tttaataaca gttcgccagc ttgcgcctgt gctttgttaa tctcatcaat cgcaaagcgt
     7261 ccgaattctc ggctgccgat attgatgacg atgtcaccac ttggcaatcc agacgattgc
     7321 ggtagacctc cgcccaaagc attaacaact gcaccgccga caactcgtcc cattgtttgc
     7381 aggaagcctg tattttccaa cggcataact acctctttac cagcctcccc aatcatggca
     7441 acggtagggc tgtccacaat accaccacgg gcaagacgag gaaggttgac gtagccaatg
     7501 ctacctaggc taacgcctgg aatgttattg attaaaccaa taacaccgtt aatcataccg
     7561 ataaagccat ttacagcgtt ctcaatagtg gcaaacacac cattcatcgc ataacgaaat
     7621 gcaccagaaa cagcagttgc tacagcgcca ccaatgttgc taaaccaact gacgatgtta
     7681 tcataaatgc cacggaaaaa tcctacaaca ttgctgaaag catttgtaat accattccat
     7741 gcctcagaaa acttctgtcc aaaccatcta cctacgttgg aaaatatatt ctcaacatct
     7801 ttccagcggt caccaaacca tttacctaaa ccttggaaga tgcggacaat agcatcccat
     7861 ccagcctgta atattgcaac aatggtattc cagacacctt ttaagaatga tagaatgacg
     7921 ttccaaacga acatgaaaat agacgacagt aaatcccaca ttccttttgc tacttgaaca
     7981 atgccgtccc aagccttttc ccagtcgcct gtaaatacac cgaccagaaa ctcgatgata
     8041 ccgcctagga tttttaaaac agctcctaaa acatcgaaaa caacattcca cgcttgcacg
     8101 aaccattctg caagtgtttg gaatattgga acaagtactg gtagtatgtt cgctgcaatc
     8161 caatcaaaga gtggcaaaaa cccctcttcc catacaactt tcaataaatc tactacttga
     8221 ccgaatgcgt ataggaaatt atcaacaaaa ggctgaatat ggttatcaaa catatcagag
     8281 aacaattgtc cgatgctatc taatactggt tgaacatcat tgttccaact gtctatcaga
     8341 gtgccaaaga tagaactgaa gccctcaatg aacgaatcaa agaacggctt taagtgttca
     8401 tcataggttg catttaagct gataaaggta ttcttgaaca agtccttgat agacgcgaag
     8461 ataggttcta cagctgataa taagccgata aatgccgttg taatacctgc ttgattatct
     8521 acggctagac gctcccaaaa cgcccaaaaa tctcgttcaa tctttccggt tatatcatct
     8581 atccccatcc ggatatagat tagtgaggag ataatggcag aaccgatatc ggtcgctgct
     8641 acgcttgtga ttgtatcgta aaaaatttga ccaatagcct gagctaaatt tccgatacta
     8701 gtgatagtgt cgccttttat ctcaaattgt ctaattagcc aattctttat atcgagctta
     8761 gtttcttttg aggatttatc tagactttcg gcaataaaga ctgcaatgcc catcacgaca
     8821 tttgcgacag ctcccgttcc ttgtcccaga gcgtaggata atttctcgcc aaaccttgct
     8881 gctgcttgca agacagttcc gtctgcaaag atgtctttta gagattgcca gataccaacc
     8941 aaagcgtttt taaaacggtc caagctgtca gctctaaacg acatattaaa gccgtcttta
     9001 aacaggtctt ttaattttga gaggtaatca aaaagaggtt gtagggtttt atcccatccg
     9061 tcaaatatgg acttaaactg gttgtccata tcagtcaatt ctatttcagg taagatgtca
     9121 gttccgttcc cagaactccc tcccttgcca tttttgccgc ccttccctcc gccggaaccg
     9181 ctaccagctc cgtctgagtc gtctttgttc aaatttaggg tagtgatttc atcgaatcct
     9241 gctaagccca gaagttcttt agcagctttc ttagctgatt tggcagtatc atctagattg
     9301 tctgcagtcc caccggacgc gtcatctaca ccgtccatgg catcgcctag accgcctact
     9361 gcgtcatttg cgttttgtgc accctgagca agatttccga ttgcgctatt cttcacgttg
     9421 gcttttttgt taaacatcaa accgataaac tcagcaagtt tagcagtgac atttttgagt
     9481 accatcgcaa aagaatttag cacaggcatc atggcattaa taatcggtaa catagcatta
     9541 ccaatattta gcgccgcatc gctcaaaagc gatttgaaca agctaatacg cccgtttaca
     9601 gactgttgca aggtattgcc gtacttggct gtcgcttgct ccaaaatagc catcaagcga
     9661 atttgttgct gggtattgta gtctaactgt tgccaagatt gcccgttcgc aaatcgctta
     9721 aaggcatttg tggactcaat catggcgaca ttgacgttga ttccaaggtc ttcaatcgct
     9781 tcggtgttcc ctagcaagcc tgatcggata cgttccataa catctgtcat ggtccgtccg
     9841 ctaccttgcg caataacagc agatgtctgt aacatcttgc ctgtataagc gctcagtttg
     9901 tcggaatctt tgataaagtt ggaaaataga ttggaataga cagctccgta cttggtagct
     9961 tctccaacac tcatattcat tgcgttggca ttgttatcaa tccactttaa aaacgcttgg
    10021 gaactttcgc ccatctgccg tttgatttga ttgactgaag cactcacttc aagagccatc
    10081 tgcgtagaat acatgccgag gtctaacatt ttcttgccta ggtaagctaa cgcagtaatc
    10141 ttagcaagtt tgcccaaagc agccgccata ccaccagctt gttgacctac acgctctcta
    10201 agaccttttg tcttgttttc aattttgttt tgagtttgct tcatctgaga ttccaattgc
    10261 ttcatctttt tttgaaatgg agcaatctcg ccttcgacaa taactctcaa ctcttctaag
    10321 gttgtagcca tagttcccct cctttccatg ttatcttatt cggaagtttt gaatcttcct
    10381 ctcattcttt cagcaaaagc cctcatccgt tcgcgatgaa ttatcaaatc tctctcaatt
    10441 cgagcttgtt ctatctgtcg cctatcctct tcgaacaaag tagggtagaa gtcccaaatg
    10501 tccggtgctt cacctttttc ctggaacatc atggaaacaa aacgagctat catttgcgat
    10561 tgaacaaagt tatgcgaagc aatttctttc tgtctctgga attgttgcct gttgtaactc
    10621 tcgaccaact ctttcaattc caaaagagtg tattcccaaa aagaaaacgg gtctacccct
    10681 gcatctaatg ctgttggata aaacccgtta atcaattctg cgaccgaaca aggaccagaa
    10741 cctactcgac tactgtcaac gtcggttctt ccttctcctt gttccgagga ataaaacccg
    10801 atacttcaaa caatggtacg atgatatcgg acatcaattc agtttgtccg tagccttcat
    10861 caatgtaatc atcgaacaag tcatagacat catccaattt gataccatga tggaacttct
    10921 gcaaagcacc ttgaacaacc aacaacatga ccttcaaagg cggtagattg aactgttcgc
    10981 ctggttgtgg cataaagatt ttgagcaaat taacgcccaa tttttcttca acttcacagg
    11041 cttggcgagt gctaagacgc aatttatagt ctgttccctt gacggtccaa gtgatatatg
    11101 gtttacgtgt tgacatttat acctccttaa agtgctactg gatcagtgaa ttctaaatct
    11161 gactgtagag ccaatttcaa tgtaaattcg attgcagaat tcacgcctcc accgcccaat
    11221 ttgactgcga tttgagccga gaaatgcacc ttcgtaccat ctgggtattc ttgttcaaag
    11281 tgacgaacag ccttggcatc tgccagttta cgcaaagtac gataactaga agttgttttg
    11341 gaattctcat acttgaattt atattccaat tcaccagcgt caccaatacc aaattcatac
    11401 tgcttaatgg tatccgcaag ggtcgtattc tcaactttct cagggtcaat accaagttct
    11461 ggaacttctt tcaagccctc gataactgta tagctagacc ctttggtctc gctcattttc
    11521 aatttaattc cattcgctaa catgttttat ccttccattc tgtattggta aacgattcgg
    11581 gagtttagat ctaaaatccc ctcaaatcgc atgactttgt gtcgtaagtg cgttgggtcg
    11641 ggtgtatcca cgctggatgt acgttttagc ccgagagatg caaaaatcgc atcaatcgct
    11701 actgctaatt ctgacgtact atcattgtgg aaaatatcga ccttgtagcg caaataggac
    11761 atctgttctg tatcatctgt aatctcgtaa ggcttgtttt cctcttctaa gtagatgata
    11821 accgggaaat tctcccaatc ctgcggataa gtatctgtca cattgtccgc aacctctttc
    11881 aatttcttgt aaatgatggg cttaatattt atcatttgct gacctcttca atcaattttc
    11941 gcttaacata tctgttgatg ttcttagtga cccgttcctc attatcctta agtgctggat
    12001 aaagatacgg ttgggcaact tgaccaaaca tcttgtaaaa ctcgccaatc ttggggaaac
    12061 gataaggacc ggcatcaatc tgagactcgt ggacatacca aggtgtgctt cgataggaca
    12121 cgctgacttc tggcgaaatg cctgcatggt ttgttgcgcc ttttggacct gttccaaatt
    12181 cgacaaatcc accatggtcc gacgtactta cgacttcagc tcttggattc ccaggcttgg
    12241 acatgcggac cttgataccc gctctcaaat caccatcgtt gacaggagct cgcaactttg
    12301 catcagcttg tacaacattc ttagcagcgt tgtgtacagc tttcgctatg atttcggtct
    12361 gtcgttgact agacaatcgt ttgagcttcg agattaacct atcagcacct atcaatcgcg
    12421 acattgttcc aactccaaga cttgatgatt tgtatatcgc tttatggata tgactttatg
    12481 ggttacttta tcgctgttga tacaaaagcc atcgccttca tctataagag tctcacggtc
    12541 taccaagcaa ttcaaaatat atgccaatct ctgaccgtat atctcagctt gtaaacgacc
    12601 actagcaggc catatctcgg cccgtatctc agtagaaaca tcgctgtaag tagctttctt
    12661 gataccctca tcactcgtca ctatgacagc tttacgaatc agatacggct tcagtcggtt
    12721 tcgcttcaaa cgcacgacct gccacccttg cgagtctatg actccgaata ccattcaaaa
    12781 tagtatctga cagcccgtct ttataagaca cagacacgcc cccttcactc cgtgatgttt
    12841 cgccctcgct tccttggcga ttgaacaact cgagtgccac ttccagttgc aaaccttcca
    12901 gcgctggcgt aagctgactt cgatttgtct cagtcaaaat gatatttttt gccctcaaaa
    12961 gcaaagacga gaggatttta tcatcactct cgcctgttaa agtttttaaa tcttctagca
    13021 tatccgcccc ctattttgca ggagcttctg ctcctttggt cttgatttct tcaatgtaat
    13081 cagccaaatt cacgtcttgc aatttagcgt ttttcttcat ctgcttatga cgttccttgg
    13141 tcaattcgat gatatcgtta acacgatgaa caaaaccaag ttcgtcatca gtaaactctt
    13201 ttaatacctt aaagcgcatt tagggcctcc tagacaatct ctttccagtt ggctgaatcg
    13261 ctaccaggag ctgttgagga tgaatttact ttcttagtgg cttcaaacaa cttaccttcg
    13321 ttctgtacac gagcaccagc ttcataagtt gcaccagata cccactgctc tgcacggatg
    13381 tttaattcgc cttgtgcgct tggcttcgct tctggtttag aggtcgcaat agaaatgatg
    13441 tacttctggt caaaatcaaa gacaaatgct ccagtataca gcaattgttc caccaactca
    13501 ccaaaacgac ctgggatgtt gttgttatac ttcgtttcat ctacttggat aggcgaaacg
    13561 actaccccgc cgattgtcgc aacagcttgt acgcctttaa ggtacttaga tggtactttg
    13621 taaaccgtga atgtatccaa ctgaccgaca taacctttgt aaagcacagt ttgattggtg
    13681 tctccttgtg gaaggttgac aatttctgac ttaatcgctt tataaaatgc tggcgtcaca
    13741 aacaacaagc ggttttccac tacgtccaat tcatccaatt tctcagaaac atccaagaca
    13801 gcttggtaag aattattagc acctttagta ttagctggta cgacattatc gctgacatta
    13861 ccaagaagag cgtcaaaacg aagatggtct aagtacggcg ccacaacttc ggcagcttga
    13921 cgagcgacaa cataattgac attgacttca ccgtttgaat cacgctcatc tagacgatcc
    13981 acaaaacgcc cccagtattt ttcttggtcc agagtgtatg tgcgttcttc tgtttgtgca
    14041 tggtcgaact cattgtcagc attacgcttg tagtctttga gttctgttgt gtcagatttt
    14101 gtaacagtaa atgaacgccc gttcatttcc actgcatcat tcgatagcaa aagcggagct
    14161 gagtaagatt ttttagcaac aactttctcg ataatcccta agaatttttc acgggatgtt
    14221 gcggtgttaa tattttcaaa tggcatattt tatttttcct ttcttatttc aaaaaatcac
    14281 gttcccactt ttcaacggtt ggttgttctt gtggcgcttt cttcatcggt gcacttcctt
    14341 tggtcttatc agcaacacct ttcaagactg ctgcttccca agtcttttga atagcatcaa
    14401 tggaatcacg tacactatca gcgtcagcaa gattaaccac gtctactaac tcgattggta
    14461 agccacgttc gcttaaaatc gtcttagctt cagcggttag ctctcggcgt gtgatttctg
    14521 cttcacggtc agcaaggtcc tgttcacgct tatcaagctg gtacttctgc ttgtcttcag
    14581 cgttcatctt ggctaatttc ttagcttctg attcagcttt ttcctgctct gctttccatt
    14641 tagcaaattc cttgtcaatg atagcatcaa catcggcatc ggtgtacttc ttttcgtctt
    14701 tcgggtctgt tgtgacttgt tcaggttcag ctgctacctg tttgtcatct tcgaccgctt
    14761 cgactgtttg tgtttcttcg ttcattgcga acctcctatt tttaaagtcg tccccgactg
    14821 tttttccata gcttttaccg tcttcaatgc ttggaccaaa aagaaaactg gtcaatttcg
    14881 accagtttaa agtaatttag agtagtttca agcagtcttt cctgctgtca agatgagtga
    14941 ccacctcctc acatagaatc caaaacactg cgaaaaccaa caatattttg gggtccaatc
    15001 gcctcataag tatcattgaa aatatcttcc ttacatggat atatttctcc gtggatacct
    15061 ttaataatat aacagccttc gtcagctcgc attacacctt ccaaagtcaa aatgtacaac
    15121 gtgtttgttt cttcgtcata gttaagttga cttctaccac aaagactgta aacatcttca
    15181 aaatttctac cgttccactg aactgcttca atttcaacag gtttcttacg ataacgcatt
    15241 ttttactcct ttatttacga ctaaaccaag acttcttgga cagcttgtta gctactttct
    15301 tttcaagata atcaaatctc gaattcgtag cctgggcgtt gcgttcgata atattctcta
    15361 gctcagccac acgattatat agccggtttt gaccgttgac gataatgtct atcgttgtag
    15421 aaatagccag tagccattgt tccaaagctt gtattagacg atatttcttt ttaatccgct
    15481 tattcatggc gttctccttt ccaaaaatta taatctttta gtgaccttac cacacttctt
    15541 gcaataataa aaatcaatct tataatgccc taaatctttg aaccaaaaat catgaacaca
    15601 taagaaagtc ttgatgaacc atttttttat tttacttagt tggtacatac tccctcctat
    15661 tttctgcaac aaaaaagcac tcgactactt gagtgcttat ttaaataatt ggtctgcctt
    15721 ctgcatatgc tttttttgct tcttctaacg tcatcttatt tggaccgccg tcaatatttg
    15781 tttcaccagt gttttcccaa ttacattcgc acacatcaaa taacatgaca ttccgtccac
    15841 aaacagggca gcgaatgtac tcttctccat caatcaggaa aatctcgttt gtagttttca
    15901 taatagtatt tctcaccttc atctggtttt aacattgtgt tcaatcgcgc ctgattattt
    15961 ttgtttcgcc gacctattac gataatgttc tcagccttat catagcgaac tctacgtcca
    16021 ccctcggtct cataacccaa aatattttca tcggtaggac tagcaatcag ttccgatgca
    16081 agttttcgat actcttcttt agtcaaactt ggaaactcat gaccgtgttt ctcaaaatga
    16141 ccattaaaag acttctcaga cgggaatttc gcttttatcc atctagcttg gtcctgtaac
    16201 tgtccatatc cctctttatc attatacttc aaatcaatat agtcttcaag cgatttaggg
    16261 gctttatctc caagaattga cttatatttc tcgtactggt tattggcacg ttcaatcttc
    16321 caaatgtcca agttatccgc cttgtatttt ggtttgacat acttctcata ccaatcttta
    16381 tacgtcatat tggcagggac ttgaatagtt ttgcctgtca ctgggtctct ggcgcttctg
    16441 gttgctttgg ctaaccattc tgaatcgtct gatgctattg tatccgaccg acaccaagga
    16501 tgcataggag gatagttctt gccagttatc cgttcactaa ctttgtagat tttaccatca
    16561 tgctctcgac agatacttga cgttctcaaa tctaatgttg cgagaaaacg ataatattcc
    16621 acatctgctt cttcataagc cttcgcttcc atttctgcat ggaaataact cgtttctgtt
    16681 cgaatcaacc gtcgagcatt ttgactccct tttccgaatt gagcctcaat gacttctgcg
    16741 gtctcgtgag ctgaccgacc cgttaacaga ctgactgcca attgcttttg taattcactt
    16801 gctaaggctt gagtattacc ccaaatcctt tcagagtaat tcgctcccag ccatggcgtt
    16861 tgctggatag ctctgatttc ctctgggtca atcctgttaa aagcaaatgc cacaccagac
    16921 tgctgctgca aatcaaaaac agagtgataa taagcatctg gaataaactc atcatagaag
    16981 gctctagaag cctcattttc ggctttatac aatcgggtgg gtaaattatc cacctcacgt
    17041 tgcaaagcct cgtatcgctc aattctggaa gcgtagggag ccaaatctaa caagataagc
    17101 aactgccgta tctcttcact gtccgttgta ttctgtaaag ctaattttaa ctcccgaata
    17161 tccgatagat tcttgacatt atctaacact cttcttgctt catcttccgt cagtccgtgg
    17221 tctctgcgat aactctcaaa aatctggtct atcttagaag tgatatgcct acttgctagc
    17281 ttgtgaattt catcgagttg ttttgcggtt tgctctgcct tgtccatttc ttgaaccatt
    17341 cgctgagctt tccgcttctc ccaatacttc tgattgtcca tctgtcacgc tctcttcata
    17401 aggcaaattc tggctaaatg caggttcttc ttgcgcagcc tctttttctt tttcaagcgc
    17461 ctcaatctct gcatctgggt cttccacaaa cggcaagagc gaaataagtt ggcgtagact
    17521 aaccttgtct ttgagattgc tgataatttg ggaaagttcc aacaaattct ttggcaaacc
    17581 acggctaaac tgcggaataa tcgccttagc attttcgtaa atctgagacc agttgtaata
    17641 attcgcaaaa atctgtatgc gtttgtgtaa agatttgata tagtatcgct ctttggtctt
    17701 ggtaatcatt tcaaggccca aaagcttaaa ttccatagct acccctgatg tattccctgc
    17761 gaaattctca tctgagagat tggggacgtg gctaaaagtg taaatatctt ctttcagcgc
    17821 cttacgaagt acttcgaccg tagcctcatc caagacattc ttcaagaact cagcacttgc
    17881 gtctttaggg agttctaaca atccttcttc tcgcagtatc tccatcgctt cttttgcttc
    17941 ttccggtgta tccgccagcg ctgcgccata taagaccaaa atagaatcaa tagcttgttc
    18001 cttgtcattc acacggttgc ccatcagtga gttatacgca tctatcaagc taatctgttg
    18061 ctcataatct ccgaccatat agcgattgtt tcgatattca ataataggca gggctccgag
    18121 attatgcgga acaccctctt ctgactcttt cttttgctca cgtaaagaca tgctatattg
    18181 caggttctct gtcaatacct gagctcgata atacgtctct tccgtcacat cgtccttcgt
    18241 ttgataataa tagaccgcaa acaaaggttt ctgttcaatc gaatcatcat aaacaataaa
    18301 agtgttctct ggttctagac tgcgagttac cagctcattt tcatcctctt tgacatagat
    18361 gtactcataa gccctgccgt agattgccat gttcaaagcg ttgtctaagt ctgtcgaatc
    18421 aacatctgca ctatcgaaag tttttagcaa atcctcgatg tccatatcgt cggtcttagg
    18481 atagcggata gcattgccca taaagtaacc tgtggcggta tccgcaatat ccttagcatg
    18541 attagcaacc gtcttaaaat ttggcagatt acttctgcgt gtgtgcttct caatagcatg
    18601 ttttccaagg tagtaatctt ttagcttctt gagtttacta ctggtctggt catgctttac
    18661 tatcaatttg taaatgatat ccttatctaa attctgctcg ttatacaacg agcgactata
    18721 gaccaatact tcttccattt ttgctccttt ctacaagccg taaagcgatt tgcgtttgac
    18781 tttggcttta gttctaattt tgtcattaat tgcttcgact acgcctgtca gtgcatctgc
    18841 agcatcatca tgagcattct taccttctct ttgatagctc ataagatttt gatataattc
    18901 tgaccaacga tgtctccaat tttcagggaa atagatatgc tctattgccc aggtcgtatt
    18961 ggtcaaaatt cttgcttgtt tattttgaga ctgatgaaac caattaaaaa ctgtatatcg
    19021 gttttggtag ctatcttgcg tcaaacgttc aacgtttcta gcgaatccgc gaccgccatt
    19081 attactttcg atgtcacatg tattgacttg ccactctgct agtttttgag ctagcaaagg
    19141 ctctgttact tccatcggtt cctttgtgaa aacaacgtcc aagatatacg cttcattgtc
    19201 catagtgacg ccgtagatat aactagctag atagtccttg cctgtatctg ccgtatcagt
    19261 gtaagcacta atacgcttaa atgtcggctt gtcaacatag gttttaaatc cactgtacaa
    19321 cctgcccttg atgtcaatag gttcttgctg gtagttcgca gacgcaatat cagctcccat
    19381 agtcttagtc ttttgaagat aagcttgttt actcaatact tcgtcacaaa gcatcgtatc
    19441 cgtagcttcg tcgtaagctt tcatgctaat gtgcttgact ttgtaatcag acttaggaag
    19501 ttcaatcagc gccttacctg ccaaatcttg cgaatgccaa cgtgtcatga taataatgat
    19561 ttttccgccc tcttcaagac gtgaaagcat cgtgttcgtg aaccactccc aatgtttttc
    19621 taaaacggtt gcgttgttag cttcttccgc attcttgata agatcgtcaa cgataataat
    19681 gtcagcaccg aaaccagtcg ctgtacctgt tggacttgta gccaagtagt tattatagcc
    19741 gccttccaaa ctccacaaat tcatagctgc atcgccatat tttatatgcg tatctggaaa
    19801 aatgtcgtta aacacaacaa cgtcttcatc tgccttcgtt tcctgaatag catttctgac
    19861 atttttcgaa aatacagttg ataacgtttc gttgtacgaa ccagtcatta tctttttatc
    19921 gttgtcattt ccaagcaacc actgaacgaa catgcctgcc gtccttgatt ttccgtgtct
    19981 gggtggttca ttgataacca acacattatg ctcgttgtca cttaaaaagc cttgtaagtc
    20041 attacaaagc tcgactaagt atctacgaga tggtttgtag aagtcgcttg ccatcaaatg
    20101 acaatagtaa aagaaatcgc gacgagctaa ctcaaaacgt gcttgttgcc tgattgccgc
    20161 tctatccatc atcaatcaac ttccttaact catccgttgt caagtcggca aatgggttgg
    20221 tttggttgat attgacttca ccatcgtgtg tcactgcttg ttttgtttta aaatcgttgt
    20281 cacgacgttc taagtaccat tttgacaagt ctatatcccc atcttcaatc gctttcgata
    20341 tattaagttt tgcccgtgtt ttcacacgtt gcttaagcaa ctcttttcgc tccgaaaatt
    20401 caggattttc cttacagtaa tcataaagag ttgtggttcc tatatcagct agaagacaag
    20461 cttcttcatc gctcaatcct ctcacaaaca actcttctaa tttccgtagt gttccttgcg
    20521 tcatcttggt tggtctacca cgctttgttt tcgccaatat catcacctcc aatcaaaatt
    20581 aaaaaagcca cacttcgttg tgtgactaat gcatattagg tcttggtccg atatgcgatt
    20641 gacaagaccg tcaatcctgt cctgattgat ctcaggtacc tgcacctgta gaccaatctc
    20701 agcagactta ttcaaattct tttgaacttg accagcataa ccactaacaa gctcgaaatt
    20761 ccgccctagc gtgtcagcca agagtctatt tgctatgttg tagtacattt taccgtctgg
    20821 caacttatcg ccagtcacag ataaactgag agcctccgcc aaaatgtctc caacttcaag
    20881 agcaaaatca tttgctgtgg cataggttgc ctttttattt ttaagtttga caaaagcaga
    20941 gctgatgatg tcactttttc cataagcaac ctcaaactta tcctgaacct ctttcagcaa
    21001 gctaggtagg atatcatcat tcatttgcta gcacctccgc tacctttgga gtggctgaca
    21061 tatctccttc aatcccggtc aaatcacgaa tggtttcggc gttgatatag ccaggtaagg
    21121 cttgattaag tttgatagca ccatcgccaa ttaaggtcaa catattggca tccgcctcaa
    21181 agagtggttc ccacttgatt tttgtattga gaaactgctc tctagtgtac tggtatctat
    21241 cccgtaaaca aacggccaca taggctacat ttaacagacc agatgcgatt gagcgttggg
    21301 cttttcgacc tgcagctctc aaattctcat gcgctgcctt tatagcttcg accgatgatg
    21361 ggttatctga tggaaaacca aggtcatcca tggttagacc gcttccaccg gcaaataatg
    21421 atgcatacat tcgcaactga tcaataaatg gtgccatgct agcagtgtta aactgaccga
    21481 cattaggact atctccgtcg tcgtcctttg taatttcaag gagactcgac actgtcgttt
    21541 tccacttctc catcgggtca gcatcttgac tagttcccaa aacatatttt tgagggaacg
    21601 agtaaaactc tgctgtcact tctgcacgct ctagggttcg cttagctgcc aactgattgt
    21661 acatgcctgc cctagtaatt cgactgcgac caaagggacg gaccgcatca ggtctgtgaa
    21721 taattggcac taataaaggt tgaccagtcg gattggagat actgtatgga tttgcacctt
    21781 tagggtaata ccatgtcaca tctttggtaa aatatgcctc taatagcggg ttatcatttt
    21841 cgtcaacttc taggatagca tatccttcgg ttagcaaaaa tgttgttgtg tcaataacac
    21901 cagtagcttt actagcttca atgacttgca ttttgggcaa gccgttttga ttaggtacca
    21961 ggtaaataaa acagcaagaa gcaatcagag ccgattggat ggcggtatcg aaaaagatgt
    22021 ctgggttatt agctgcgaag atctcagtcg cttcaaaatc atcatttgta aattcacgaa
    22081 atataattct gtctgctaag gcgtccacac tgtgtgctgt ccatccgata acagactcat
    22141 acatgctttt gacgctatcc gggatgataa aacttcttgt attatctcta tctgtcatag
    22201 catagaaccg atagcgtttc ttaactcctg gctgatacat agctaacttc tttcggagat
    22261 aactcattcc tttgtaactc attttgattg ctccttaatt ttatttatca cttgctcttc
    22321 aaagtctggt tcatgaattt ccacaccttc tataacggtc ctgctagtaa atccaaactc
    22381 tgcctcatta gctaaaattt tggttttgaa atcttggtaa aatttcagca agtttttctt
    22441 tttcttacgt ttttttgtaa gtttgatgtt acgatttgac ttgtcatctt tttccttttt
    22501 tgcttcagcg cgtagtcttt tcatctggag tcgttttttc tcacgattcc catcactacg
    22561 gcatttatca ctgcaatatt ttgagcgact tgtttttctg acaaatgatt taccacagat
    22621 ttcacattta atttttccca ctttctccct cccaaatcgt aacgcgaaaa aaaatgtaca
    22681 gtgacggcgt gaagtccggc cgaacgaatt ggtggggttc tacccccctt cagattttgc
    22741 acagataaac tttgaccaat cttgactctg aggaagattt ctatttccca ctgtttttgg
    22801 ttctgcttta gaattatctg cgaataactt atcagattta attctattgc aagacatgtg
    22861 agccaactgc agattttcaa tcaatgatgg atgaccgcct tttgccaacg gaaccacatg
    22921 atcgattact gcacacattg gatttggata ttttaatttc ttattaacta actgaccaca
    22981 tattccacaa tgtgttgcag tcttaagtat tctcttctta ttcttttcaa aagctactct
    23041 atgcgttccg ctcttgtctg ctctcatcgt tacctcaaac aaaagaggcg ccg
//
LOCUS       DAZQCJ010000024        20584 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000024 DAZQCJ010000000
VERSION     DAZQCJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20584
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.024"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(79..396)
                     /locus_tag="ACHU66_000852"
     CDS             complement(79..396)
                     /locus_tag="ACHU66_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFR3389149.1"
                     /translation="MNIDEKDLLPIMQLIMYSGDAKGHGMRAIQVAKEGCFDEADDLL
                     RQAHDSLIQAHHVQTDMLTREAAGDTLSIGLLMIHSQDHLMNAITFLDLAREMIDLYK
                     KLG"
     gene            complement(406..723)
                     /locus_tag="ACHU66_000853"
     CDS             complement(406..723)
                     /locus_tag="ACHU66_000853"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR3389150.1"
                     /translation="MKRIVLACSAGMSTSLLVTKMQQAAEAQGFEATIYAVSVSELVL
                     EVEKNPADIILLGPQVSYMEEEFKKDPHLANIPIAVINMLDYGTMNGAKVIEYAKSLL
                     DLS"
     gene            complement(851..1084)
                     /locus_tag="ACHU66_000854"
     CDS             complement(851..1084)
                     /locus_tag="ACHU66_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR3389151.1"
                     /translation="MLTSIIAGAIIGLIAGALTSSDDRRGCLGNIILGLAGSWIGQLL
                     FGDWGPQFAGMALVPSVFGAVLLVAIFSRSRRD"
     gene            complement(1143..2384)
                     /locus_tag="ACHU66_000855"
     CDS             complement(1143..2384)
                     /locus_tag="ACHU66_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR3389152.1"
                     /translation="MVLPNFKENLAKYAKLLVSTGINVQPGHTVQLTIGVEQAELARL
                     IVKEAYAHGAKEVLVNWLDDVIARERLVNVDVELLEQVHPQRITEMNYLLERKASRLV
                     VLSEDPGAYDGVDPEKLSRNTRALSQALNPMRQASQANKISWTLGAASGLEWAKKVFP
                     NAASDEEAVDLLWDQIFKTCRIYEEDPIKAWEEHEARLVAKAKVLNDEQFVKLHYTAP
                     GTDLVLGMPKNHLWEAAGSVNAQGEHFIANMPTEEVFTAPDYRVADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGEIVDVTAEKGDEVMKKLVFDNAGARGLGEVALVPDKSPISQSGV
                     TFFNTLFDENASNHLAIGQAYAFSIEGGTEMIQEELKEAGLNRSDVHVDFMIGSNKMN
                     IDGIREDGTRVPIFRDGEWAI"
     gene            complement(2881..3483)
                     /locus_tag="ACHU66_000856"
     CDS             complement(2881..3483)
                     /locus_tag="ACHU66_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFR3389153.1"
                     /translation="MSNFAKLQEIRRTIYALGKDLPVSNEEVVALVEKAMKESPSAFN
                     SQSSRAVVLFGAESEAFWNEIAYSELEKVTPAEAFEATKGRLAGFAAGAGTILFFEDQ
                     DVVKGLQESFPLYAENFPIWSEQAHGINLYAVWLAFAEKNIGMNVQHYNPLVDAQVAE
                     KYGIPANWKLRAQAPFGGIAAPAADKDYMADSDRVKVFGN"
     gene            complement(3718..4389)
                     /locus_tag="ACHU66_000857"
     CDS             complement(3718..4389)
                     /locus_tag="ACHU66_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFR3389154.1"
                     /translation="MKKRNILVELLKGIGLVLLSTILWTLILSFATPESSSGIYEPFP
                     GFTVIAGILTSFILHIIMKYNQLRQAEQAAKASKSHIVVIEERNQALMKKANRLVEKH
                     QNLEKETILDINKSVSKIRTVEEKTVMKSTKIESSQEFGQFLRQMPELMANKHIERLL
                     NEIFETENNLAQWKISYNQQVEYFNTLLHQFPISVIAKIIRLKDLEFYKEHSLTDFTD
                     EMLGL"
     gene            complement(4401..8792)
                     /locus_tag="ACHU66_000858"
     CDS             complement(4401..8792)
                     /locus_tag="ACHU66_000858"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFR3389155.1"
                     /translation="MSDKFQLLLQQIGMPLDARQSGAFSTATIEKVVLHKVSKLWEFT
                     FRFETPLPLMDYQLFKARLATEFEKVGNKIQFSIVSDAEAFEAGLVEAYYPEAFTEDL
                     CQSAGFKALFQPLEVAYRDGVLWIKGPETIDTDHFRKNHLPNLVEQYKRFGFGNLAVD
                     IQVCQEMTQQQAEIFHAQNEEIYQQANEENLAALEQLAQMAPPPEAAQPFVPEYKKNR
                     PAKVNIEKAEITPMIEVDSEENRIVFEGLVFEVEQKTTKTGRVIINFKMTDYTSSFTL
                     QKWAKNEEEAQKFDMVKKGNWLRVRGNVETNNFTRDLTMNVQEVQEVKKEIRKDLMPE
                     GEKRVEFHAHTNMSTMDALPAVEDLVARAAAWGHKAVAITDHGNVQSFPHGYHAARKA
                     GIKPLFGMEANIVEDSVPIAYNEADVVLSDATYVVFDVETTGLSAVNNALIQIAASKM
                     HKGNIIAEFDEFIDPGHPLSQFTTDLTGITDEHVRGSKPLEQVLREFQDFCQDSVMVA
                     HNATFDVGFMNVNYERAGLPIISQPVIDTLEFARNLYPDFKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFLKDALEKHNLTNLNQLNTELIAEDSYKKARVKHATLYVIN
                     QVGLKNMFKLVSLSNTKYFEGVPRIPRTVLNAHREGLILGTACQEGEVFDDLLSKGID
                     EAVKTAAYYDFIEVMPPALYAPMIAKEQFKDKAEIEETIKQLIEVGRRAGLPVLATGN
                     VHYIDPEEEIYREIIVRALGQGAPINWTIGNGENAQPAPLPKAHFRTTSEMLDEFAFL
                     GESLAREIVITNPNAMLDRFEDVQVVKTDLYTPYIEKAEETVAELTYQKAFEIYGNPL
                     PDIIDLRIEKELTSILGNGFAVIYLASQMLVHRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPMPPHYVCPNCQHSEFITDGSYGSGFDLPDKDCEKCGTKYKKDGQDIPFETFLG
                     FDGDKVPDIDLNFSGDDQPSAHLDVRDIFGEENAFRAGTVGTVAAKTAYGFVRGYERD
                     YGKFYRDVEVERLAAGAAGVKRTTGQHPGGIIVFPDYMDVYDFTPVQYPADDVTASWQ
                     TTHFNFHDIDENVLKLDILGHDDPTMVRKLQDLSGIDPQTIPADDKGVMALFSGTEIL
                     GVIPEQIGIPTGMLGIPEFGTNFVRGMVEETKPTTFSELLQLSGLSHGTDVWLGNAQD
                     LIKAGIANLSTVIGCRDDIMVYLMHAGLPPKMAFNIMERVRKGLWLKISEEERNGYIQ
                     AMKDNKVPDWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPLYYYCAYFSIRAK
                     AFDLATMSGGLERVKAKMEEIALKKKNNEASNVEQDLYTTLELVNEMLERGFKFGKLD
                     LYKSHATDFLIEDNTLIPPFVAMDGLGENVAKQVVAARAEGEFLSKTELRKRGGLSGT
                     LVEKMDEMGILGKMPEDNQLSLFDDLF"
     gene            complement(8916..9905)
                     /gene="dhaQ"
                     /locus_tag="ACHU66_000859"
     CDS             complement(8916..9905)
                     /gene="dhaQ"
                     /locus_tag="ACHU66_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HFR3389156.1"
                     /translation="MVFIRNRQATIIEDYLDGFCRTNPKVKKVKGLPIVLQKVQDDHL
                     VPIISGGGSGHEPAHIGFVGKGMLTAAVYGEIFTPPTAEEVLAAIRAVDKGKGVFLIV
                     KNFEADLVSFRKAIELARQEGIAVKYIVSHDDISVDTKNNFRMRHRGLAGTILLHKIL
                     GAAALTGYSLDELEQLGLSLATEIATIGFATKSAYLPNAALPLFDLEEGQISYGIGIH
                     GEEGYRTVRFESSEQLANEIVNKLKLKFRWKEGEDYILLVNNLGSMSEVEQGIFLNDI
                     CQFLELEGLQLSYIKTGKFMTSLDMGGISVTLCRVKDRKWLDFLEAPTEAAAW"
     gene            complement(9916..10461)
                     /gene="dhaS"
                     /locus_tag="ACHU66_000860"
     CDS             complement(9916..10461)
                     /gene="dhaS"
                     /locus_tag="ACHU66_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HFR3389157.1"
                     /translation="MPTSLITKKRIAKAFKKQLSQKSFDKISVVDIMQEAGIRRQTFY
                     NHFLDKYELLDWIFETELQEQVTDNLTYISGIKLLDELLYYIESNQSFYRQLFEIKGQ
                     NDFVSYLEGYFMVLMEKIINEYQLQEGKMISQQDKHFLILYHANAWIGLIKEGLTQSP
                     CQIHSYWKQMVALVRESFVIL"
     gene            10609..11598
                     /gene="dhaK"
                     /locus_tag="ACHU66_000861"
     CDS             10609..11598
                     /gene="dhaK"
                     /locus_tag="ACHU66_000861"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFR3389158.1"
                     /translation="MKKIINQPEHVVDEMLKGLVFMHQDLVDRIDGFDVIIRKAEKTG
                     KVGIISGGGSGHEPSHAGFVGKGMLSAAVCGAVFTSPTPDQILEAIKAADEGAGVFMV
                     IKNYSGDIMNFEMAQELAEMEGIEVDSVVVDDDIAVENSLYTQGRRGVAGTILVHKIL
                     GHAAETGKSLAEIKELADRLVPQIKTIGLALSGATVPEVGKPGFVLEEDEFEYGVGIH
                     GEPGYKKEKLQPSAKLAEELVEKLAEGFDIQDGEHYGVLINGLGATPLMEQYVFANDV
                     AQLLAKKGVVVDYKKIGNYMTSIDMAGLSLTLIKLEDPSWIDALQAPVEVVAW"
     gene            11609..12184
                     /gene="dhaL"
                     /locus_tag="ACHU66_000862"
     CDS             11609..12184
                     /gene="dhaL"
                     /locus_tag="ACHU66_000862"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFR3389159.1"
                     /translation="MDAKQALLWMETFNKKIQEDKDYLSELDSPIGDGDHGGNMARGM
                     AAVMQELSEKDYETADQVFKVVAMQLLSKVGGASGPLYGSAFMGITKASQAGADLADT
                     LQAGLEMIQKRGKAELGEKTMVDVWIPVIAALRESQLTTEIIHKAVQGTKDIAATKGR
                     ASYVGERSIGHIDPGSFSSGLLFEAMLEAGL"
     gene            12181..12555
                     /gene="dhaM"
                     /locus_tag="ACHU66_000863"
     CDS             12181..12555
                     /gene="dhaM"
                     /locus_tag="ACHU66_000863"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFR3389160.1"
                     /translation="MTKVGILLVSHSKNLAQGIIDLVSEVAKDIPITYCGGLEDGSIG
                     TSFEIVQERVEANPADTLLAFFDLGSARMNMELAADFTDKQILIQTVPVVEGCYTAAA
                     LLQAGADLETILEQLKELTINK"
     gene            12565..13275
                     /locus_tag="ACHU66_000864"
     CDS             12565..13275
                     /locus_tag="ACHU66_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFR3389161.1"
                     /translation="MNLIGELLGTFILVLLGNGVVASCILSKTKAENSGWLTIVLGWG
                     IAVTIAAYVSGVFGPAHLNPAVTIAMASIGSLPWAEVPGFLLAQFAGAMLASIVLYLH
                     FYPHWQETKDSGTILATFSTGPALRHTASNLLGEALGTAVLVMGILAIGPNNVSAGLG
                     PIIVGLIVFAIGFSLGSTTGYAVNPARDLGPRIMHAILPIPNKGDSDWSYSWIPVAGP
                     ILGGVAGAILYNLLLNML"
     gene            complement(13613..15475)
                     /locus_tag="ACHU66_000865"
     CDS             complement(13613..15475)
                     /locus_tag="ACHU66_000865"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR3389162.1"
                     /translation="MKQSKMIIPTLREMPSDASVISHALMLRAGYVRQISAGIYSYLP
                     LANRVIEKAKNIMREEFDKIDAIEFLAPALLSADIWRESGRYETYGDDLYKLKNREGS
                     DFILGPTHEETVTLLARDAVQSYKQLPLNIYQIQSKYRDEKRPRNGLLRGREFIMKDG
                     YSFHASYESLDQTYDDYKAAYEAIFTRAGLEFKAIIGDGGAMGGKDSQEFMAITPDRT
                     DLDRWVVLDKSVASFEEIPEDVLEAIKEELLAWSVSGEDTIAYSSESGYAANLEMATS
                     EYKSSTAVVVEEELVKVATPDAKTIDEVAAFLNVAEEQTIKTMLFMADGEPVVALLVG
                     NDQVNDVKLKNHLGANFFDVAGPADAEKIFGAGFGSLGPVGLPENIKIIADRKVQDVK
                     NAVVGANEDGFHYTGANAGRDFQVTEYVDIREVKEGEPSPDGHGVLNFARGIEIGHIF
                     KLGTRYSDSMNANILDENGRSMPIIMGCYGIGVSRLLSAVLEQHARLFVNKTPKGEYR
                     YAWGINFPKELAPFDVHLIPVNVKDEAAMELTQSIEASLVGAGYEVLTDDRNERVGVK
                     FSDSDLIGLPIRVTVGKKAADGIVEVKIKGTGDTVEVHVDQLLETLQILTKENE"
     gene            complement(15759..17018)
                     /gene="rseP"
                     /locus_tag="ACHU66_000866"
     CDS             complement(15759..17018)
                     /gene="rseP"
                     /locus_tag="ACHU66_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFR3389163.1"
                     /translation="MKGILAFIFIFGVIVVVHEFGHFYFAKKAGILVREFAIGMGPKI
                     FAHTGKDGTLYTIRILPLGGYVRMAGWGEDKTEIKTGSPASLSLNEAGVVTRINLSGK
                     QLDSLSLPMNVTSFDFEEKLEITGLVLEESKTYKVDHDATIVEEDGTEVRIAPLDVQY
                     QNATVWGRLMTNFAGPMNNFILGILVFILLFFMQGGVANPSSNAVSITEGGALQAAGV
                     VTGDKILSVNGNTTDSYTEVATIISKAATDATTAPSFDLVVEHDGKNRHVSVTAEQVD
                     GAYRIGISPILKTGFVDKIIGGFQEAGATALRVVTALKNLIANFDVKQLGGPVAIYKV
                     SSQAAEFGLVSVLGLMAALSINLGIFNLIPIPALDGGKIVMNILEAIRRKPLKPETES
                     YITLAGVAVMVVLMIVVTWNDIIRVFF"
     gene            complement(17028..17834)
                     /locus_tag="ACHU66_000867"
     CDS             complement(17028..17834)
                     /locus_tag="ACHU66_000867"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR3389164.1"
                     /translation="MTNDLQKRVIFGGVALAIFIPFLLAGGMAFQFFVGLLAIIATAE
                     LIKMHRLAPNSIEGVLAMLASLVLALPLQNYLTFLPTDGNYTAYAIVVFLLLGSTVFN
                     IGHYNYSDVVFPIASSFYVGIGFHSLILARMDGLNKVFFALCLVWATDIGAYLIGRQI
                     GRRKLLPKVSPNKTVEGFAGGILSAVVVAIIFLIVDKSLLAGYSFGMMLLLVVIFSIF
                     SQFGDLVESAIKRHFGVKDSGKIIPGHGGILDRFDGMIFVFPIMHFFGLF"
     gene            complement(17844..18599)
                     /locus_tag="ACHU66_000868"
     CDS             complement(17844..18599)
                     /locus_tag="ACHU66_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFR3389165.1"
                     /translation="MFKFNFKKKESIETAIEVPKHIAVIMDGNGRWAKKRMQPRIMGH
                     KAGMDALQKVTKTASDLGVKVLTVYAFSTENWSRPEKEVKFIMNLPVEFYDKYVPELH
                     ANNVKIQMIGDHSKLPVPTLNALYKAEQKTKSNTGLILNFALNYGGRDEVTRAVKAIA
                     QDVLDAKFNPGDIDEKLIADYLYTGTLSPTLRDPDLVIRTSGELRLSNFLPWQTAYSE
                     LYFTDIAWPDFDGEALKLAIKEYNRRHRRFGGV"
     gene            complement(18713..19036)
                     /gene="yajC"
                     /locus_tag="ACHU66_000869"
     CDS             complement(18713..19036)
                     /gene="yajC"
                     /locus_tag="ACHU66_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR3389166.1"
                     /translation="MEGLFLPLVMVAMIGFMFYSQRKQQKQRQEALNQIKKGDEIVTI
                     GGLFGIVDEIDDKKVVLDVDGVYLTFERGAIRNRVASSGTTSAVVEEVAVETTEATPE
                     AAIEE"
     gene            complement(19146..20438)
                     /locus_tag="ACHU66_000870"
     CDS             complement(19146..20438)
                     /locus_tag="ACHU66_000870"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR3389167.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGTKYKLHLIPSGIFFPEKISVIGNGVVVNPKSLVKEINYLHDSGVTTDNLRISDRA
                     HVILPYHIKLDQLQEESKGENKIGTTNKGIGPAYMDKAARVGIRIADLLDKEIFAERL
                     RTNLAEKNRLFEKMYESTPIEFDEIFEEYYAYGQEIKKYVTDTSVILNDALDQGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELHDEIGDRIREIGKEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLETLKICVAYDLDGERIDHYPASLEQLKRCKPIYEEMPGWSEDITGVRSLDELPEA
                     ARNYVRRISELVGVRISTFSVGPGREQTNILESVWSAK"
BASE COUNT         5805 a         4945 c         4106 g         5728 t
ORIGIN      
        1 ccgcagattg cttgaatcta cggcttttta tttaatattc tcttttttaa accgacaact
       61 aattgaaaca attctcattc accccaattt tttatataaa tcaatcatct ctcgtgccaa
      121 atctaaaaaa gtaattgcat tcattaaatg gtcttgacta tgaatcatta gtaaaccaat
      181 ggaaagggtg tcacccgctg cttctcttgt caacatatcc gtctggacat gatgtgcttg
      241 aattaacgaa tcgtgagctt gtctcaataa atcatctgct tcatcaaagc aaccttcctt
      301 agctacttga atagctctca tcccatgacc cttagcatct ccactataca tgatgagttg
      361 cataataggc aataagtctt tctcgtctat attcatcact atctcctatg ataaatctag
      421 tagagatttg gcatattcaa ttaccttagc cccgttcatc gtaccataat caagcatatt
      481 gataactgcg ataggaatat tagctaaatg tggatctttc ttaaactcct cctccatata
      541 actaacttgt ggtcccaaca aaataatatc tgctggattc ttttcaactt ctaataccag
      601 ctcactcacc gaaacagcat agattgtagc ttcaaaccct tgagcctcag cagcttgttg
      661 catcttagtc accaataagc tagtactcat accagcagag cacgcaagta caattcgttt
      721 catttttgat tctcctataa ataattatct attgatatgg cgataggatc gtacgttgtt
      781 ataacaacta taacattttt aaaaactttt tgcaagcgct ctcttagaca atttttcaat
      841 cagtgcagtt ctaatccctc cgactgcggc taaaaatagc caccagcaat actgctccaa
      901 agactgacgg taccagggcc ataccagcaa actgtgggcc ccaatcacca aagagcaact
      961 gtccaatcca tgaacctgca agaccaagga taatatttcc tagacagcca cgacggtcat
     1021 ctgatgaggt caaggctccg gcaatcaagc caataatagc tccagcgata atgcttgtta
     1081 acattctgta ttcctcctta tttacaaaat aagactggtt gcccagtctt ataagatgac
     1141 tattagatag cccactcacc gtcgcggaag atagggacgc gagttccatc ttcacgaatg
     1201 ccatcaatgt tcattttgtt tgaaccaatc atgaagtcaa cgtggacatc agaacggttg
     1261 agacctgctt ctttcaactc ttcttggatc atttctgtac caccttcaat tgagaaggca
     1321 taggcttgcc cgattgccaa gtggtttgag gcattttcat caaagagagt gttgaagaaa
     1381 gttacacctg attgagagat tgggctctta tctggtacaa gggcaacttc acctagaccg
     1441 cgtgcaccag cattgtcaaa gaccagtttc ttcatgactt cgtcaccttt ttcagcagtc
     1501 acatccacga tttcaccatc tttgaaggtt actttgatgc cttcgatgat gttaccattg
     1561 taactgagtg gttttgttga cgttacatag ccgtctgcca cacggtagtc aggagctgtg
     1621 aagacctctt ctgttggcat gttggcaata aagtgttcac cttgggcatt aaccgaacca
     1681 gccgcttccc acaagtgatt cttcggcata cctagaacga ggtcagttcc tggtgctgtg
     1741 tagtgcaatt tgacaaactg ctcgtcgtta agcaccttag ccttagccac caagcgtgct
     1801 tcgtgctctt cccaagcctt gattggatct tcctcgtaga tacggcaagt cttgaagatt
     1861 tggtcccaaa ggaggtccac tgcctcttcg tcagacgctg catttgggaa gactttttta
     1921 gcccattcca aaccagaggc tgcaccgagt gtccagctga ttttgttagc ttgggatgct
     1981 tgccgcattg ggttcaaagc ctggctgagt gcacgagtgt tgcgagacaa tttctctgga
     2041 tcaacgccat cgtaagcacc tggatcttct gacaaaacaa ccaaacggct agccttacgc
     2101 tccaagaggt agttcatttc agtgatgcgt tgtggatgaa cttgttccaa gagttccaca
     2161 tcaacattga ccaaacgttc acgcgcaatc acatcgtcca accagttgac caagacttcc
     2221 ttagcaccgt gagcgtaagc ttctttgaca atcaagcgtg ccaactcagc ctgctccaca
     2281 ccaattgtca attgaaccgt gtgaccaggt tggacattga tacctgttga aaccaagagt
     2341 ttagcatatt tagcgagatt ttctttaaaa tttggtaata ccatcttttt ctcctttgta
     2401 ctttgaaata ccctattatt gtatcatatt ttaagctaga cagataaaaa aacttcctgc
     2461 tgatacagga agttgaaaag gttagctctg ggagatcgtt gaagcttgga gatgaaacga
     2521 atggagttcg tgtcagcaag ccgcaggtat aactcaacac cctcttattt ctaggcattg
     2581 acttgaaata gtcccccaga ttgcttacac agttaatgga ctgtgcaagg tgggagataa
     2641 aacttgcgga gcaagtcgct tttgcagaat caggcaaggc gagttcaaac aatccagtgg
     2701 attgtttgaa gttggaaata aggaaacgaa gtttcctcaa ctgccgacgt aataaagcaa
     2761 actgaaataa attatatata ctcaatgaaa attggaatca gactaggcga cgtagatgta
     2821 gatagaactt aagttcatca catcaagtca acaacgtctg acttcaattt tcgcagagta
     2881 ttagttacca aaaaccttta cacggtcact atccgccata tagtctttat ccgctgctgg
     2941 agctgcaatg ccaccgaatg gtgcttgggc acggagtttc cagttagctg ggataccgta
     3001 tttttcagca acttgtgcat caaccaatgg gttgtagtgt tgtacgttca taccgatatt
     3061 tttctcagca aaggccaacc atactgcgta caagttgata ccatgagctt gttcagacca
     3121 gattgggaag ttttcagcgt aaagtgggaa gctttcttgt aagcctttca ccacgtcttg
     3181 gtcttcgaag aacaagatag taccggcacc agccgcaaaa ccagctaagc gaccttttgt
     3241 agcttcaaaa gcttctgcag gcgttacttt ttccaattcg ctgtaggcaa tttcgttcca
     3301 gaaagcttct gactccgcac caaaaagtac cactgcacgg ctagattgtg agttgaaagc
     3361 tgatggagat tctttcatgg ctttctcaac aagtgctact acttcttcat ttgatactgg
     3421 caagtcttta ccaagtgcgt agattgtacg gcgaatttct tgaagttttg caaagtttga
     3481 catatatttt cctcttttct tattgttact acatagtagt ttttatcact atagggagat
     3541 tgtatcatta ttactaagta gtgtcaaatt cctagtctaa cttttttata ctccgtgaaa
     3601 attaagctag ttacagcaga tagtctgatt ttcataaagt agtgacagag aatttacaaa
     3661 aagatggcaa aaaagcctat caaatactta agatacattg atagaccctt ctttatctta
     3721 tagccccaac atctcgtctg taaaatcagt cagcgaatgt tccttataaa attccaagtc
     3781 cttcaggcga atgattttgg caatgacgct aatcggaaac tgatgcaaca gagtattgaa
     3841 atactcaacc tgctgattgt atgaaatctt ccattgtgct aaattatttt ctgtttcaaa
     3901 aatttcattc agtaaccttt caatatgttt attggccatc aattccggca tttgacgcaa
     3961 aaattgacca aattcttgtg aactttcaat cttggtggat ttcataacag ttttttcttc
     4021 aaccgttcga attttggaaa cggatttatt gatgtccaaa atggtttctt tttcaagatt
     4081 ctggtgtttt tcaaccaagc ggtttgcttt cttcatcaag gcctgattgc gctcttcaat
     4141 aacaacgata tgacttttac tagctttcgc ggcttgttca gcctgacgaa gctggttata
     4201 cttcataatg atatggagaa tgaaactcgt tagtatacca gcaatcacag taaaacctgg
     4261 aaaaggttcg taaatacctg atgatgattc cggtgtggca aaagataaga tcaatgtcca
     4321 caagatagta gaaagcagta ctagaccaat tccttttaat aactcaacta aaatatttct
     4381 ttttttcatc atttcctttt ttaaaacaaa tcatcaaaga gactcaactg gttatcctct
     4441 ggcattttac cgaggatgcc catttcatcc attttttcaa caagcgtacc cgacaatcca
     4501 ccacgtttac ggagttctgt cttggagaga aattctcctt cggcacgcgc cgccacaacc
     4561 tgcttggcaa cgttctcccc aaggccatcc atggcaacaa atggtgggat gagggtatta
     4621 tcttcaatca agaaatcggt tgcatgggac ttgtaaaggt ccaatttccc gaacttgaag
     4681 ccacgttcca gcatttcatt taccaattca agtgtggtat agagatcctg ctcaacgttc
     4741 gaagcttcat tgtttttctt cttgagggcg atttcttcca tcttagcctt gactcgttcc
     4801 aagccaccgg acatggttgc caaatcaaat gccttggccc gaatagagaa ataagcgcaa
     4861 tagtagtaaa gtgggtgatg aaccttgaaa taggcaaccc gcaaggccat cataacgtag
     4921 gcagccgcgt gggctttagg gaacatatac ttgatttttc cacaggattc gatgtaccag
     4981 tctggcacct tgttatcctt catggcttga atgtagccat ttcgctcttc ctcagaaatc
     5041 ttgagccaca agcccttccg cacccgctcc atgatattaa aggccatctt aggtggcaaa
     5101 cctgcgtgca tgaggtaaac catgatgtca tcccgacaac cgataacagt agatagatta
     5161 gcaattcctg ccttaatcaa gtcctgggca ttgcccaacc aaacgtctgt accgtgcgat
     5221 agaccagata actgcaagag ttccgaaaaa gtggtcggtt tggtttcctc caccatcccc
     5281 cgtacaaagt tggttccaaa ttcaggaatg cccagcatac ctgtcggtat cccaatctgc
     5341 tctgggataa cacccagaat ttccgtacca gaaaaaagag ccataacccc cttatcatca
     5401 gcaggaatag tttgcggatc gatgcctgac aagtcctgca acttccgaac catggttgga
     5461 tcatcgtgcc ccagaatatc gagtttcaag acgttttcat caatatcgtg gaagttaaag
     5521 tgggtggtct gccagctggc tgtcacatca tcggcaggat actgaacagg ggtaaagtca
     5581 tagacatcca tgtagtcagg gaatacgata attcctcctg ggtgctgacc agtcgtccgt
     5641 ttgacaccag cagcaccagc agccaggcgt tctacttcca catctctata gaatttgcca
     5701 taatcccgct catagcctcg gacaaaacca taggccgtct tggccgccac cgtaccaacc
     5761 gttcctgcac ggaaggcatt ttcttcaccg aaaatatccc gaacatccaa atgggcagac
     5821 ggttggtcat cccctgagaa gttcaagtca atatcgggaa ccttgtctcc atcgaaacca
     5881 aggaaggtct cgaaaggaat gtcctgtcca tcttttttat acttggtccc acacttttca
     5941 caatccttgt ctggcaggtc aaaacctgaa ccataggaac catctgtgat aaattcactg
     6001 tgttgacaat ttggacagac atagtgagga ggcatagggt ttacttcggt aatcccaatc
     6061 atggtcgcaa cgaaactgga gccgacagac ccccgtgaac ccaccaggta gccccgctcg
     6121 ttggaacggt gcaccaacat ctgcgatgcc aggtaaatca cggcgaatcc attacccaga
     6181 atggacgtca attccttctc aatccgcagg tcgataatat ctggaagagg attgccataa
     6241 atttcaaagg ctttctgata ggtcaattcc gcaaccgttt cctcggcctt ctcaatataa
     6301 ggcgtataaa ggtcggtctt aaccacctga acatcttcaa accggtccag catggcattg
     6361 ggattggtaa tgacaatctc acgagccaga ctttctccca agaatgcaaa ctcgtccaac
     6421 atctcgctgg tcgttctaaa gtgggctttt ggcagtggtg ctggctgagc gttctcacca
     6481 tttccgatgg tccagttaat cggtgcccct tgacctagag cacgaacaat gatttcccga
     6541 tagatttcct cttcggggtc aatgtagtga acatttcctg tcgccagaac aggcagacct
     6601 gccctacgtc ctacctcaat caactgctta atagtctctt caatctctgc cttgtcctta
     6661 aactgctcct tggcaatcat gggagcatag agggccggtg gcatgacttc gataaaatca
     6721 taataggctg ccgttttaac cgcttcatct atccctttgg agagcaagtc atcgaagacc
     6781 tcaccctctt gacaggctgt tcccaatatt aaaccttcac gatgggcatt gagaacggtc
     6841 cgtggaatac gtggaactcc ttcaaaatac ttggtattgg aaagagagac cagtttgaac
     6901 atatttttca aaccaacctg attaatcaca tagagcgtgg catgcttgac acgagccttc
     6961 ttataggaat cctccgcaat cagctccgta tttagctggt tgagattggt caaattgtgc
     7021 ttctccaagg catcttttag gaagatgaag agcaggcgcc ctgtcgcctc cgcatcgtag
     7081 ttagccatgt ggtggtgttc aagagcaaca ccaaaccgct tggtgagagg acctaaacca
     7141 tgacgcttaa agtcgggata aaggttacga gcaaattcca aggtatcaat gacaggctgg
     7201 ctaataatag gtaagcctgc tcgttcatag ttgacattca taaagccaac gtcaaaggtc
     7261 gcgttgtggg caaccatgac ggaatcttga cagaaatctt gaaactcacg cagaacttgt
     7321 tctagaggtt tagaaccacg cacatgctca tctgtgattc ctgtcagatc tgttgtaaat
     7381 tgactgagcg gatgacccgg atcgataaac tcatcaaatt ctgcaatgat attgcccttg
     7441 tgcatcttag acgccgcaat ctgaatcagg gcattgttaa ctgcagagag acctgttgtt
     7501 tccacgtcaa agaccacata ggtggcatca gacaggacta catctgcttc gttgtaagca
     7561 ataggcacag agtcctcgac aatattagcc tccatcccga agagcggctt aatcccagct
     7621 ttacgggcag catgatagcc atgggggaag gattgaacat tgccgtggtc ggtaatcgcc
     7681 accgccttgt gtccccaggc tgctgcacgc gccaccaagt cctcaacggc aggcagggca
     7741 tccatagtag acatgttggt atgggcatga aactcaaccc gcttctcacc ctcaggcata
     7801 aggtccttac gaatttcctt cttgacctcc tggacttcct gtacgttcat agtcaaatca
     7861 cgagtgaaat tattggtctc cacattgcct cgcacccgca accagttgcc ctttttgacc
     7921 atatcaaact tctgggcctc ttcctcattc ttagcccact tctgcaaggt aaaggacgaa
     7981 gtatagtcgg tcatcttaaa gttgataatg actcgccccg tcttggtcgt cttctgctcg
     8041 acctcaaaga ccagtccctc aaagacaatc cgattctcct cgctatccac ctcaatcata
     8101 ggcgtaatct cagccttttc gatattgacc ttggcaggac gatttttctt atattctggg
     8161 acaaatggct gagctgcctc tggtggtggc gccatttgag ccagttgttc aagggccgcc
     8221 aagttttctt cattagcctg ctggtaaatt tcttcatttt gcgcatggaa aatctccgct
     8281 tgttgctgcg tcatttcctg acagacttgg atgtccaccg caagattacc aaaaccaaat
     8341 cgcttgtatt gctccacaag atttggcaga tgattcttcc gaaaatgatc tgtgtcaatg
     8401 gtttcaggac cttttatcca caaaacacca tctctataag ccacctctaa tggctgaaaa
     8461 agagccttga agcctgcact ctggcacaaa tcttccgtaa aagcttctgg ataatatgct
     8521 tctaccaaac cagcttcaaa agcctccgca tcgctgacaa tagaaaattg aatcttattg
     8581 cccacctttt caaactccgt cgccaaacga gccttgaaga gttgatagtc catgagcggc
     8641 aagggggttt caaaacggaa ggtaaattcc caaagtttac tgaccttgtg caagactact
     8701 ttctcaatcg ttgcagtcga aaaagcccct gattgtcgtg catcaagagg cattccgatt
     8761 tgttgtagca agagttgaaa cttatctgac atacttattt cctttgtagt tcgttagaaa
     8821 cattttacca taaatgaggt attttttcct gagtcaggaa gaaaaaactc tcaggtcatt
     8881 tcctgagaga tctgatttct atagaaatta gtagcctacc aagccgcagc ttctgtaggc
     8941 gcctctaaaa aatccagcca ttttcgatcc ttaacacgac agagggtgac agaaattcct
     9001 cccatatcaa gactggtcat aaatttacct gtttttatat aggaaagctg taaaccttct
     9061 agctctagaa attggcaaat gtcattcaaa aagattcctt gctcaacctc agacatagat
     9121 ccaagattat tgaccagaag aatatagtct tccccctcct tccagcggaa ttttaatttt
     9181 aatttgttca caatttcgtt ggctaactgc tcagaggatt caaaacgaac ggtccgatag
     9241 ccttcttctc catggatacc gatgccgtaa gatatttgtc cctcctccaa atcaaacaaa
     9301 ggcagggcag cattgggcaa ataggccgac ttggttgcaa aaccgatggt cgcaatctct
     9361 gttgccagag acagtcccag ctgttccaat tcatctaggc tatagccagt aagggcagca
     9421 gctcctaata tcttatgaag tagaatggtc cctgcaagcc ccctatgccg catacggaaa
     9481 ttattcttag tatcgaccga aatatcgtca tgggatacga tgtacttgac tgcaattccc
     9541 tcctgccgag ccaattcgat ggcttttcta aaagagacaa ggtctgcctc aaagtttttt
     9601 acaattagaa aaactccctt acccttatct actgcacgaa tggctgccaa tacttcttca
     9661 gcggttgggg gcgtaaatat ttcaccatat actgctgcag tcagcatacc ctttccgaca
     9721 aatccaatat gtgccggttc atgccccgat ccaccacctg aaataattgg aactaaatgg
     9781 tcatcctgca ctttctgaag cacaatcgga agtcccttca ctttcttcac tttaggattg
     9841 gttcgacaga aaccatccaa ataatcttca ataattgttg cctgtctatt tctaataaac
     9901 accatatatt aactcctaca aaatcacaaa cgactcgcga acaagagcca ccatttgctt
     9961 ccaataactg tgaatctgac aaggtgactg tgtcagtcct tcctttatca gtccaatcca
    10021 agcattagca tgatagagaa tcaaaaaatg cttatcctgt tgggaaatca ttttaccctc
    10081 ctggagctgg tactcgttga taattttctc catcaaaacc ataaagtagc cttctaaata
    10141 gctgacaaaa tcattttgcc ccttaatctc aaagagttgc ctatagaaag attggttact
    10201 ctcgatgtag tacaaaagct catccagaag cttgattcca ctaatatagg tcagattgtc
    10261 agttacctgc tcctgcaatt ctgtctcaaa tatccagtcc agcaactcat acttgtctaa
    10321 aaaatggttg taaaaggttt gacgacgaat ccctgcttcc tgcatgatgt cgacaactga
    10381 gattttatca aaggattttt gggatagctg ttttttaaaa gcctttgcaa tccttttctt
    10441 tgtaatcaac gaggttggca tatgtcactt cctttcgctt cctctttatt ataccctatc
    10501 atgcttttct ttggaacaaa agggacacat tggacaatct gtcccttcct tttttctttt
    10561 ggacaatgta gaatgaaagc gcaaacaaaa aaacaaagga gttttctcat gaaaaaaatt
    10621 atcaatcaac ctgaacatgt cgttgatgaa atgttaaaag gacttgtctt tatgcaccaa
    10681 gatcttgtcg atcgtatcga tggatttgat gtgattatcc gcaaggctga aaaaactggt
    10741 aaagtcggca tcatctcagg aggtggctct ggtcacgaac cttctcatgc aggctttgtc
    10801 ggcaagggca tgttgtccgc cgctgtatgt ggcgcagtat ttacctcacc aacaccagac
    10861 caaatcttgg aagccatcaa ggctgctgac gagggagctg gcgtctttat ggtcatcaaa
    10921 aactactctg gcgacatcat gaactttgag atggcgcaag aattggcgga aatggagggc
    10981 attgaagtgg acagtgtcgt tgtggacgat gacatcgctg tggaaaacag cctctatacc
    11041 caaggacgtc gtggtgtagc aggtaccatc cttgtccaca agattctcgg tcacgctgca
    11101 gaaacgggca aatcgctggc tgagattaag gaactggctg accgtctcgt cccacaaatc
    11161 aagactatcg gcttagccct atcaggtgct acggtaccag aagtgggcaa accaggcttc
    11221 gtccttgaag aggatgaatt tgaatacggt gtcggtatcc atggtgaacc tggctataag
    11281 aaagaaaaac tccaaccatc tgctaaattg gctgaagaat tggttgagaa attggcagaa
    11341 ggctttgata ttcaagatgg cgagcactat ggtgtcctta tcaacggtct cggcgcaacc
    11401 cctctcatgg aacaatatgt ctttgccaac gacgttgctc aattgctggc taaaaaaggt
    11461 gtggtcgttg actacaagaa aatcggtaac tacatgacat cgattgatat ggctggactg
    11521 tcattgacct taatcaaact agaagaccca agctggattg acgctttgca ggcccctgtt
    11581 gaagtggtag cttggtagga gaaagactat ggatgcaaaa caagcactgc tctggatgga
    11641 gacattcaac aaaaaaattc aagaagacaa agattatttg agtgagctag attctccaat
    11701 tggagacgga gatcacgggg gcaacatggc gcgaggaatg gctgccgtca tgcaggagct
    11761 atctgagaaa gactatgaga ctgctgacca agtctttaag gtggttgcca tgcaacttct
    11821 cagcaaggtt ggcggtgctt ccggtcctct ctacggctct gcctttatgg gtattactaa
    11881 agccagtcaa gctggcgctg atttggcaga tacgcttcaa gcaggtttgg agatgatcca
    11941 aaaacgcggc aaggctgaat tgggagagaa aaccatggta gacgtgtgga ttcccgtcat
    12001 tgctgcccta agagaaagcc agctgacaac ggaaattatc cataaagccg ttcaaggtac
    12061 aaaggatatt gctgcgacca agggacgcgc gtcctatgtt ggggaaaggt ctattggaca
    12121 cattgatcca ggctctttct catctggact tctctttgaa gccatgttgg aggcaggact
    12181 atgacaaagg ttggtatctt actcgtatcc cattcaaaaa atttggccca gggaatcatc
    12241 gacctagtat ctgaagtcgc aaaagatatt cccatcacct actgtggcgg actagaagat
    12301 ggtagcatcg gtaccagctt tgaaatcgta caagagcgag ttgaagcaaa ccctgctgat
    12361 accctccttg cctttttcga cctaggtagc gcccgaatga acatggaact tgctgccgat
    12421 tttacagaca aacaaattct tatccaaacc gtaccagtcg tcgaaggttg ctatactgcc
    12481 gcagctctct tacaggctgg agctgacttg gagactattc ttgaacaatt aaaagaactg
    12541 acaatcaata aataggaggt cattatgaat ctgattggag aattacttgg aactttcata
    12601 ctagtattac ttggaaacgg tgtggttgca tcctgcatcc tctctaaaac caaggctgaa
    12661 aatagcggct ggctgaccat cgtactagga tggggcatcg ccgtaacaat cgctgcctac
    12721 gtatcaggag tttttggacc tgcccacctg aatccagctg tcactatcgc aatggcatct
    12781 atcggctccc taccttgggc tgaagtccca ggcttcctac tcgctcaatt tgcaggagca
    12841 atgttggcat ccatcgtcct ctatctccat ttctacccac attggcagga aacaaaagat
    12901 agcggaacaa tccttgctac cttctctact ggaccagctc ttcgtcacac agcttcaaac
    12961 ttgctcggcg aagctcttgg tacagctgtc ttagtcatgg gaatccttgc aattggtcct
    13021 aacaatgtca gcgctggcct cggcccaatc atcgtgggac ttatcgtatt tgcaatcggc
    13081 ttctcactag gatcaacgac aggctacgca gtcaatccag ctcgtgactt aggccctcgt
    13141 atcatgcacg ctatccttcc aattcctaac aagggagatt ctgactggtc ttatagctgg
    13201 atcccagttg ctggcccaat tcttggaggt gtcgcaggag caatcctcta taacctccta
    13261 ctaaatatgt tataaaaaaa taggcttttg gaaaaagacc agcgcaactt atcagagttc
    13321 gtatcaacat ctcagcgcag tggttgattg gcagatttat tcgtgtttca cactccaaat
    13381 ctgacctata cgactgaagc gaacagagtt cgcttcatct ccaacctcaa actatctccc
    13441 agacagtttg agctgtgcgg gggtgggagt gaaacagtct ggggatagac tgtttcagct
    13501 caacaactta aaacgaaaaa ttgttgacga actctgtttg acctagtcga gttctttccc
    13561 actcccattt tatcaacaaa gacttggcta tcatcaacca agtctttttc tgttattcat
    13621 tttcttttgt aaggatttgc aaggtttcaa ggagttggtc cacatggact tctacagtat
    13681 cacctgtgcc cttgattttc acttcgacga tgccatcagc tgctttctta ccaacagtca
    13741 cacggattgg caagccaatc aagtcactgt cagaaaactt aacgccgaca cgctcattac
    13801 ggtcgtcagt taatacttca tagcctgcac caaccaaact tgcttcgata gactgagtca
    13861 attccatcgc cgcttcatct ttgacattaa ctggaatcaa atgcacatca aacggtgcca
    13921 attctttagg gaagttgatg ccccaagcgt aacggtattc gccttttggt gtcttgttaa
    13981 caaagaggcg agcgtgttgt tcaagaacgg ctgacaagag acggctaaca ccgattccgt
    14041 agcatcccat gataatcggc atagaacgac cattctcatc caagatgttg gcattcatac
    14101 tgtctgaata gcgtgtaccg agtttgaaga tgtgaccaat ctcgatacca cgggcaaagt
    14161 tgagaacccc gtgtccatct ggagaaggct cgccttcctt gacttcgcga atatccacat
    14221 actcagtcac ttggaaatca cgccctgcat tggcacctgt gtagtggaaa ccgtcttcgt
    14281 tggcaccgac aacagcattt ttcacatctt gaaccttgcg gtctgcaata attttgatgt
    14341 tttctggcaa gccgactggt ccgagggaac caaaacctgc cccgaagatt ttttccgcat
    14401 ccgctgggcc tgctacatca aagaaatttg ctccaaggtg gtttttcaac ttgacatcgt
    14461 taacctgatc attgccaaca aggagggcaa caactggctc accgtctgcc ataaagagca
    14521 tagttttgat agtttgctct tcagcgacat tcaagaaggc cgcaacttcg tcgatggttt
    14581 tggcatctgg cgtcgctact ttaaccaatt cttcttcgac gacaactgct gtacttgatt
    14641 tgtactcgct agttgccatt tctaggttgg cagcataacc agattcgcta gagtaggcaa
    14701 tggtgtcttc accagacaca gaccatgcca agagttcttc cttgattgct tcaagaacat
    14761 cctctgggat ttcttcaaaa gaagcaacag acttgtccaa gacaacccaa cggtccaggt
    14821 cagtacggtc tggtgtaata gccataaact cctggctatc cttaccaccc atggcaccac
    14881 cgtcaccgat aatggcctta aactccaagc ctgcacgagt gaagattgcc tcgtaagccg
    14941 ccttgtagtc atcgtaggtc tggtccagac tctcatagct agcgtggaag ctgtagccat
    15001 ctttcatgat gaactcgcga ccacggagaa gaccgttacg agggcgtttt tcatcacgat
    15061 acttagattg gatttggtag atgttaagcg gcaactgctt gtaagactgg accgcatcac
    15121 gcgccaaaag agtcaccgtt tcttcatgag ttggtcccaa gataaagtca gaaccctcgc
    15181 gatttttgag cttataaagg tcatcaccgt aggtttcata acgacctgac tcacgccaaa
    15241 tatccgcgga caagagggct ggtgccaaaa attcaatagc atcaatcttg tcaaactcct
    15301 cacgcatgat attttttgct ttttcaatca cacggttagc caaaggcaag tagctgtaaa
    15361 taccagcaga aatctgacgg acatagcccg cacgtaacat caaagcgtgg ctaatcaccg
    15421 acgcatccga aggcatctcc cgcaaagtag ggataatcat tttagactgt ttcatagtta
    15481 agatataagt gcgttaagaa aacgaaacga aaataggaaa cctaacgcag tgcttttggc
    15541 actaggcagg tttatctttt tcgtaaagtt tttagcacgc attcaattcc tttcttatat
    15601 tcatcatcag gaaatccaga ggcgtagctc tcaatctatt ttccgagttt ttagaccgag
    15661 ttcaattacg aacctcctta attcctttca tcttttgttt atcaggaatg cacaggtctc
    15721 agcccgtgtt caattccttt cataaatttc aaattatctt agaagaagac tcttataata
    15781 tcattccaag tgactactat cataagaacc accataacgg ctacacctgc tagagtgata
    15841 taagattccg tctctggttt gagtggtttt ctgcgaatgg cttccagaat attcataaca
    15901 atcttaccac catccaaggc tggaattgga atcaaattga agattccaag gttaatggag
    15961 agcgcagcca ttagccccaa tacagaaacc aaaccaaatt cagctgcttg agaacttact
    16021 ttataaatag caaccggtcc ccccaactgc ttaacatcaa agtttgcaat tagattcttc
    16081 aaagctgtta caaccctaag agctgtcgct cccgcttctt ggaaaccacc gataatttta
    16141 tcaacgaaac cagttttcaa aatcggtgaa ataccaattc gataggcccc atctacttgt
    16201 tcagcagtaa cggagacatg acggtttttc ccatcatgct caacaaccaa atcaaagctt
    16261 ggggctgttg ttgcatctgt cgctgctttg ctaataatcg ttgcaacttc tgtgtagctg
    16321 tctgtcgtgt tcccattgac cgaaagaatc ttgtcccctg tgacaacacc tgcagcctgc
    16381 aaagcaccac cttcagtaat acttactgca ttgcttgatg ggttggcaac cccgccttgc
    16441 atgaagaaca aaagaataaa tactagaatc cctaaaataa agttattcat gggacccgca
    16501 aagttggtca tcaaccgtcc ccagactgtc gcattttgat actgaacatc aagtggagca
    16561 atccgaactt ctgttccatc ttcctctaca atcgtggcat cgtgatcaac cttataggtc
    16621 ttgctttctt ccaagaccaa gcctgtgatt tccaattttt cctcgaagtc aaaactggtt
    16681 acattcattg gcaaactgag actatccagt tgcttaccag acaaattaat ccgcgtcaca
    16741 acaccagctt cattcaggct gaggctggca ggactacctg tcttaatctc tgtcttatct
    16801 tcaccccatc cagccattcg aacatagcca cctaggggaa ggatgcggat ggtgtaaagt
    16861 gtcccatcct tacctgtatg agcgaaaatt ttgggaccca tcccaatcgc aaattccctc
    16921 acaaggatac cagctttctt ggcaaagtag aaatgaccaa attcatggac aaccacaatc
    16981 acaccaaata taaatataaa tgctaaaatt cccttcataa tacctcctta aaataatcca
    17041 aagaagtgca tgattggaaa gacaaaaatc ataccgtcaa aacgatccag gataccgccg
    17101 tgtcctggaa taatttttcc agaatccttc acaccaaaat ggcgtttaat agcactttct
    17161 actaaatctc cgaattgtga gaatatcgag aaaatcacaa ctaacaacaa catcatgcca
    17221 aatgaatagc ccgccaacaa ggacttatcg acaatcaaga aaatgatagc aactaccacc
    17281 gcagaaagaa taccgcctgc aaagccttca accgttttat tcggtgaaac cttcggcaat
    17341 aattttctcc gaccaatctg acgaccgatt aagtaggcac caatgtctgt tgcccagact
    17401 aaacagaggg caaagaagac tttattgagc ccatccatac gagccagtat caaactatga
    17461 aagccgatac caacatagaa acttgatgca attggaaaga ctacatctga atagttgtaa
    17521 tgaccgatat taaaaaccgt cgaaccaagc agtaaaaata ctacaatagc gtaggctgtg
    17581 taatttccat ccgtcggtaa aaatgtcaga taattctgga gcggaagagc caagactaaa
    17641 ctggccaaca tagctaagac accctcgata gaatttggtg ctaggcgatg cattttaatc
    17701 aattcagccg tagcaatgat ggctaacagg cctacaaaaa attgaaatgc catcccacca
    17761 gccaacaaaa agggaatgaa aatagctaga gcaacgccac caaatatcac tcgtttctgt
    17821 aaatcatttg tcataccttc tccttataca ccgccaaaac gtctgtggcg acggttgtat
    17881 tctttaatcg ctaatttaag tgcttctcca tcaaaatcag gccaagcaat atctgtaaag
    17941 tacagctcgc tgtaggctgt ttgccacggc aggaagttgc taagacgaag ttcaccactt
    18001 gtacggataa ctagatcggg atcgcgtaac gttggtgata aggttcctgt atagaggtaa
    18061 tccgctatta atttctcatc aatatcccca ggattgaact tggcatccaa gacatcttgc
    18121 gcaatagcct tgactgcgcg agtcacctca tcacgaccgc catagttaag agcgaaattg
    18181 agaatcaaac cagtgttact ctttgtcttt tgttcggctt tataaagcgc attgagagtc
    18241 gggacaggaa gttttgaatg gtcaccaatc atctgaattt tgacattatt ggcatggagt
    18301 tcaggaacat acttatcata aaactccaca ggtagattca tgataaactt cacttctttt
    18361 tccggtcgag accagttttc tgtagaaaag gcatagacag ttaacacctt tactcccaaa
    18421 tcagaagccg tcttggtaac cttttgaagt gcatccatac cagccttatg ccccataata
    18481 cgtggctgca tccgcttttt cgcccaacgg ccattgccat ccataatcac agcgatatgc
    18541 ttagggactt caattgcagt ttcaatgctt tctttctttt taaaattaaa tttaaacatg
    18601 gtattttcct tattgtataa tcgtttcatt ataccatatt tttacctagg atagcaaaaa
    18661 tcaggcaggg ctaaaacaag agaaaaccaa ggtttccctc ggtttttcat ccttattctt
    18721 caattgcagc ttctggagtt gcttctgtcg tttcaactgc cacttcttca acaactgcag
    18781 atgtagtacc tgaacttgct acacgattac gaatagcacc acgttcaaat gtcaagtaaa
    18841 caccgtcaac atcgagaaca acctttttat catcgatttc atcgacaata ccaaatagcc
    18901 caccaatggt cacaatttca tcaccttttt tgatttgatt tagtgcttct tgacgttgtt
    18961 tttgttgttt acgttgcgaa tagaacataa aaccaatcat ggcaaccatg acaagcggca
    19021 aaaataaacc ttccatttgt ttacctcttt tattgtttga taagcatgat tatatcaaat
    19081 tctggacaag aaagcaaagt tcgaccaaac agaaaaagac cgaactttcg ttcagtcttc
    19141 gcacactatt ttgcgctcca tacactctca aggatgttgg tctgttcacg accaggtcct
    19201 actgagaagg ttgagatacg aacgcctacc aattcgctga tacgacgaac atagttgcga
    19261 gccgcttctg gcaattcatc caggctacgt acacctgtga tgtcttcaga ccaacccggc
    19321 atttcttcgt agattggttt gcaacgtttg agttgctcca aacttgctgg gtagtggtca
    19381 atacgctcac catccaagtc ataagccacg cagattttca aggtctcaag acctgacaag
    19441 acgtcaatcg agttgaggga caagttggta atacctgaca cacggcgact atggcgcatc
    19501 accactgagt caaaccaacc gacacggcgt ggacgcccag ttgtcgtacc gtattcctta
    19561 ccgatttcgc ggatacggtc tccgatttca tcgtgcaatt cagtcgggaa tggtccgtca
    19621 ccaacacggc tagtgtaggc cttacataca ccaacaacct tatcaatctt gcttgggccg
    19681 acaccgctac cgatcgtcac accaccagca actgggtttg aagaagtaac gaatggatag
    19741 gtaccttggt caatatccaa cataacccct tgcgcacctt caaataagac acgcttgcct
    19801 tgatcgaggg catcgttcaa gataacagag gtatctgtta catacttttt gatttcttga
    19861 ccataagcgt agtattcttc aaagatttcg tcaaactcaa tcggagttga ctcatacatt
    19921 ttctcaaaca aacggttttt ctcagccaag tttgttctca aacgctccgc aaaaatctcc
    19981 ttgtccaaga ggtctgcaat acggataccg acacgcgcag ccttgtccat gtaggctgga
    20041 ccgataccct tgttggttgt accaatctta ttctcacctt tagactcttc ttgcaactgg
    20101 tccaatttga tgtgataagg caagatgaca tgtgcgcggt ctgaaatccg caaattatct
    20161 gttgtcacac ctgaatcatg gagatagttg atttccttca ccaaagattt tggattgaca
    20221 accacaccat taccgattac agaaatcttt tctgggaaga agattcctga cggaatcaag
    20281 tgcaatttat acttcgtgcc atcgataacg atagtgtgtc cagcgttatc accaccttga
    20341 taacgtgcga ttacttcagc attagcagac aagaagtctg taattttacc cttaccttcg
    20401 tcgccccact gggttcctac aacaactact gatgtcatat tcactctttc tctgaactgg
    20461 ctaaccagcc cagcctttct tctacttctg gcaggaatct cacctgcaag tttcgtttta
    20521 cacactattc tatcaaaaaa cgaacatttt ttcaagaaat tccaataatt tcatatatat
    20581 cgaa
//
LOCUS       DAZQCJ010000025        20509 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000025 DAZQCJ010000000
VERSION     DAZQCJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20509
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.025"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..709
                     /locus_tag="ACHU66_000871"
     CDS             <1..709
                     /locus_tag="ACHU66_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389168.1"
                     /translation="NSLFWEWRCLTTSSQFYIYLILSGMYYLLLALLNFFEVGFGEYL
                     LKGPGLDGIETISLLSYLLFQAQNIFLLQQLFSGLSTTNFNLTILRIGSIKDWFLVKL
                     VSLIFLSLLLGLFQTVPLFFIGNMDYLFFLHFFVVNSTNFILFSSLLFLLFHFDFSDA
                     VSFCITYSLLVFIIFSGEILGENTNYFVTILARVAEYEKNSNLSLFIAIFFEIILSAA
                     ILSFIKIRYSLGGKIHE"
     gene            702..1343
                     /locus_tag="ACHU66_000872"
     CDS             702..1343
                     /locus_tag="ACHU66_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3389169.1"
                     /translation="MNNSIIKIENLSKKFKNCDLFDNLHFECSEGEIVGITGINGSGK
                     SVLFKLIAGLMQPNSGTIKVYGEDIVGKIPSGLGALIEEPGFIPNYSGFENLEFLSTI
                     QNIIGKEEILATLHRVGLFEDKDKKVRNYSLGMKKKLGIAQAIMEEPRILLLDEPTNA
                     LDSQSVQRVYDLLKELSKEKGVTVLLASHNDSDIRHLCDKVYLLENKQCKLLK"
     gene            1598..1867
                     /gene="rpsO"
                     /locus_tag="ACHU66_000873"
     CDS             1598..1867
                     /gene="rpsO"
                     /locus_tag="ACHU66_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR3389170.1"
                     /translation="MAISKEKKNEIMAQYARHEGDTGSVEVQVAVLTWEINHLNDHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRRTDVNRYRELIHSLGLRR"
     gene            1882..2007
                     /locus_tag="ACHU66_000874"
     CDS             1882..2007
                     /locus_tag="ACHU66_000874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389171.1"
                     /translation="MSSDIGSFSYVPLWKCSARKKLGVLRLIEEYVIRSVVYIAG"
     gene            2170..4389
                     /gene="pnp"
                     /locus_tag="ACHU66_000875"
     CDS             2170..4389
                     /gene="pnp"
                     /locus_tag="ACHU66_000875"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR3389172.1"
                     /translation="MSKQVFETVFAGKKLVVETGQVAKQANGAVVVRYGDSTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGWMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDPDASAPMAAMFGSSLALAISDIPFNGPIAGVQVGYVNGELI
                     INPDKAQMEESLLDLTVAGNKDAINMVESGAKELSEDIMLEALLKGHEAIQELLDFQN
                     QIVAAVGKEKADVELLQVDPELQAEIVAAYNDDLKKAVQVEEKLAREDATNAVRETVI
                     AAYEEKYAEHEEFDRIMRDVHEILELMEHAEVRRLITEDKVRPDGRRVDEIRPLDAEV
                     DFLPNVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLGDEYKKRFLHHYNFPQYSVGS
                     TGRYGAPGRREIGHGALGERALEQVLPSLEDFPYAIRLVAEVLESNGSSSQASITAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIDGITPQILEEALAQAKKARFEILDVIEATIPEVRPDLAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEDGLVAIFSPDRDAIERTKEIIAGLVREAKV
                     DEVFQAKVVRLEKFGAFVNLFDKTDALVHVSEMAWTRVNKPEDLVEIGDIVDVKVIKI
                     DDKGRIDASMKALLPKPEGYVEPEKRERSDRPRRHKDNKEKKDNNFGEFKFHKVEKK"
     gene            4443..5060
                     /gene="cysE"
                     /locus_tag="ACHU66_000876"
     CDS             4443..5060
                     /gene="cysE"
                     /locus_tag="ACHU66_000876"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR3389173.1"
                     /translation="MGWWKDTIEIVKEKDPAARTTLEVLLTYPGVKALAAHRLSHWMW
                     KKGFKLLARMHSQFWRFWTNIEIHPGADIASGVFIDHGAGLVIGETAIIESGVMLYHG
                     VTLGGTGKDTGKRHPTVRKGALVSAHAQVIGPIEIGENAKVGAAAVVLADVPADVTVV
                     GMPAKIVRVHGQKDEKVIHDMEDGREHYTTKLAELREASHRSSHL"
     gene            5075..5848
                     /locus_tag="ACHU66_000877"
     CDS             5075..5848
                     /locus_tag="ACHU66_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HFR3389174.1"
                     /translation="MLLEEFENTSAVIEPTDMAIRGGGEVCETMIFSFNGEIVDRVRQ
                     LPESREGGYLESINGRHPWYILERDGVRVAVMLAVIGAPMAVGHLEELKACGFENFIV
                     LGSCGVLDKSLVADKIILPSSALRDEGTSYHYAPASDEISYDPALLLTMEKALDQAGI
                     EHVRAKTWTTDAFYRETSAKVKRRLAAGAMVVDMEASAIMAWANFRQAKVYQFFYTAD
                     YVDYHKNEWDARREERTADSMTFFEVAMAIARKLESTSC"
     gene            5842..6027
                     /locus_tag="ACHU66_000878"
     CDS             5842..6027
                     /locus_tag="ACHU66_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389175.1"
                     /translation="MLKKLVRQNWPYVLTAIAGTIMFILKFSQGSWQVGMIWLAATAY
                     WLVKLYQKYQVLKNTQK"
     gene            6038..7381
                     /gene="cysS"
                     /locus_tag="ACHU66_000879"
     CDS             6038..7381
                     /gene="cysS"
                     /locus_tag="ACHU66_000879"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR3389176.1"
                     /translation="MIKIYDTMTRSLRDFVPLEEGKVKMYVCGPTVYNYIHIGNARSV
                     VAFDTIRRYFEYRGYDVTYISNFTDVDDKIIKAANEAGMTTKELSDKFIAAFKEDVAK
                     LGVKPATKNPRVIDYMGEIIDFVQVLIDKGYAYEASGDVYFRVEKAENYARLANKTLS
                     DLEAGASGRVEGEGQLKEHPFDFALWKTAKPGEVSWESPWGAGRPGWHIECSVMATEI
                     LGATIDIHGGGADLEFPHHTNEIAQSECKTGQTFANYWMHNAMLNINDEKMSKSLGNF
                     LTAHDMLEQIDGQVLRFFLSTQHYRRPLNYTEKAISDAEINLKYLKNTYTQPVQNSVD
                     KSALELHLAAFEAAMDDDFNAANGITAIFDFAKWINSGNYDETVKEAFGKMLEVFGIV
                     FEEEVLDSEIEALIAERQAARANRDFATADRIRDELAAQGIKLLDTKDGVRWTRD"
     gene            7374..7772
                     /locus_tag="ACHU66_000880"
     CDS             7374..7772
                     /locus_tag="ACHU66_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFR3389177.1"
                     /translation="MTKAVDVNLINGIALAFEGDAVYSMYVRRHLIFKGLTKPNKLHG
                     EANKYVSAKAQAGLISALLEAQLLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGF
                     EAVLGYLHMTEQTERLDELVGWCIDNIERG"
     gene            7774..8628
                     /locus_tag="ACHU66_000881"
     CDS             7774..8628
                     /locus_tag="ACHU66_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR3389178.1"
                     /translation="MNEKELAGWFPQAKLSQEADAQSLALGDGRYLNLADADLTERER
                     YLLSLLSHPQEQKPSSPWQSYLHGQSSLPQAVEAIQFIHVHLWSQKDAQLDAWQTMMT
                     DLLPSQLAQVQLSSQDYLFIIEQSRWTDYQTILADTLSALEFDFGLRLTFLVGQVWSR
                     QLADRWSQLYQAEAALFAHWRAHYHQSTVLSFSQLFLWGQGRAGLEFGMIKASMLQLI
                     DQQDMQDIILALWTEGAVVTKTAQILYIHRNTLQYRLDKWQDLTGLQLKDLTDLSFCY
                     QVLLDRLF"
     gene            8738..9868
                     /locus_tag="ACHU66_000882"
     CDS             8738..9868
                     /locus_tag="ACHU66_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3389179.1"
                     /translation="MVQLNLKNIYKKYPNSEHYSVENFNLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGEAYIDGVLMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYSKDEIKKRVEEAAQILGLTEFLQRKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRTEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNEAGT
                     GTIGRIEQIGSPEELYNKPVNKFVASFIGSPAMNFFTVTLQEGVLVGDGFKVDLPEGR
                     RKHLEEKGYNGKSFTLGIRPEDIKASQLELDTYPSSIVEAEVVVSELLGAETMLYSKV
                     GNTEFVSRVDARDYHNPGDKVRLTFNLNKAHFFDDETDQAIV"
     gene            complement(9908..10876)
                     /locus_tag="ACHU66_000883"
     CDS             complement(9908..10876)
                     /locus_tag="ACHU66_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFR3389180.1"
                     /translation="MSLSLRTIKLIFATVLAIYLATVLGLSYTTAAGIIAILSVLDTR
                     KSSFKMARNRLFSTLLALTIAVLTFALFGFGIWTLGIYLALYVPLAYRFNWEAGIAPS
                     TVLVTHLLLEQDISLIFLGNELALFLIGAGLALLFNLYMPSQEKKIQAYHDQVEDLLK
                     QILLRFEAFLLNGDGRNEAELITQLDQTLEQALKVVYLDRHNQLFQQTNYQVHYFEMR
                     AAQNKILRTMAGNINKCLLEGRENVILSSLFERAAQQLSRENSAKELLLDIELFHATF
                     RERPLPQTREEFETRATLFQLLHDMEAFIRLKVDFYEVYKDEKDPS"
     gene            complement(10951..11342)
                     /locus_tag="ACHU66_000884"
                     /pseudo
     CDS             complement(10951..11342)
                     /locus_tag="ACHU66_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730574.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
     gene            11480..12208
                     /gene="rlmB"
                     /locus_tag="ACHU66_000885"
     CDS             11480..12208
                     /gene="rlmB"
                     /locus_tag="ACHU66_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR3389181.1"
                     /translation="MEHNDIVYGVHAVVESLEANTGNKLYIQDDLRGKNVEKIKALAA
                     EKKVSISWTPKKTLSDMTEGAVHQGFVLRVSEFAYADLSVILEKAEQEDNPLILILDG
                     LTDPHNFGSILRTADATQVAGIIIPKHRAVGVTPVVAKTSTGAVAHVPIARVTNLSQT
                     LDKLKEAGFWVFGTDMNGTPSHKWNTAGKLALIIGNEGKGISSNIKKQVDEMITIPMK
                     GHVQSLNASVAAAVLMYEVFRKKI"
     gene            12209..12730
                     /locus_tag="ACHU66_000886"
     CDS             12209..12730
                     /locus_tag="ACHU66_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFR3389182.1"
                     /translation="MKEKVLIVDGYNMIAFWQATKQDFKKGDLDAARTTLLRTLSHYA
                     AFEQIEVICVFDAHHVPGLRQQYDEFKVSVIFTEEEETADSYIERLSAQLNSDRRKQV
                     SVATSDLNEQWVVFSQGALRVSARELEERTRIIKKDLDKFVDQVELYTPRLNPWSDGN
                     FQALKEMMEEMKE"
     gene            12727..13590
                     /locus_tag="ACHU66_000887"
     CDS             12727..13590
                     /locus_tag="ACHU66_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFR3389183.1"
                     /translation="MTFTIVTDSTSDLPISWVQENDVTVLGLTINLDGVTYETVGENR
                     LTSADLLEKMASGSQPTTSQVNVGQFEEVFEEAAKEGHEVLYLAFSAALSGTYQSAVI
                     AREMVVDQYPDAVITIVDTKAATIGEGYLVMKAVEARAAGKTLAETKAIVEDLVPRLR
                     TYLLVDDLNHLVRGGRLSKAAALIGGLVNIKPLLALNAEGKLEAIAKIRGRKKGIKEM
                     LNLTLDNLDHSTVMVAYTGDIEAAEGVKSSLLEDDRISDVLLTELGPVIATHTGTGVL
                     AILSIGTAMRA"
     gene            14054..14500
                     /gene="rplM"
                     /locus_tag="ACHU66_000888"
     CDS             14054..14500
                     /gene="rplM"
                     /locus_tag="ACHU66_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR3389184.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKSISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            14520..14912
                     /gene="rpsI"
                     /locus_tag="ACHU66_000889"
     CDS             14520..14912
                     /gene="rpsI"
                     /locus_tag="ACHU66_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR3389185.1"
                     /translation="MAQAQYTGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTQGSYDVFVNVNGGGYGGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(15021..16241)
                     /gene="xerC"
                     /locus_tag="ACHU66_000890"
     CDS             complement(15021..16241)
                     /gene="xerC"
                     /locus_tag="ACHU66_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR3389186.1"
                     /translation="MTITKTKNGTYRLKVYIPTETRMPLGIVNNNYFDKRFKTRKEAR
                     QAEIDLLTKINQIENNEFTGIGKGDILFKDFYESIWWEAYKAGQTTSTTKPPSKSTVA
                     NTKTCFRKHILPMLGNYTVQFLNQNKQVILNLMAAKASEYANFKTLRSYVISIFDWAE
                     ELEYIESNKIAKTLRRIKSTKKIQLAEARRDEDLYLTQEQLQEWFSAFQDDLASGKIS
                     LKDYVLFYLTFFLGDRKSESYALQWKHIDFEKSQIQLLQALDRYGDVKSTKGNKKTIF
                     AISTDLLQLLQGWKSQQKQELAKFGIITNPEQFVFTYIDTKGNINKPLHADYLNNKMK
                     TIKKRHPKLTHATPHKLRHTGATLAKKAGMSLEAISEALTHSDTLTTKTYVNTSNIVP
                     LSAGHVAYQHLKNE"
     gene            complement(16253..16570)
                     /locus_tag="ACHU66_000891"
     CDS             complement(16253..16570)
                     /locus_tag="ACHU66_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR3389187.1"
                     /translation="MQVILPDDQIKQIQILISSLITSEIEKQIHHRNIDNSYLNKKQA
                     CDYLGISNNTLDEWIRKGLPSIKIGKTIRFSKQAIDSWLLSQNYKYPHFCFIMVMIFS
                     GST"
     gene            17149..17859
                     /locus_tag="ACHU66_000892"
     CDS             17149..17859
                     /locus_tag="ACHU66_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5986 family protein"
                     /protein_id="HFR3389188.1"
                     /translation="MKYSYDIEREVIIKCLEAMSFTTRHLEQDYKVAISETSQNGIYQ
                     ATWARRSNMLKDLFDNSVVWKVLHIKRGIWQFDPILNIETGELIAFFSKNNFRTIRNR
                     YFKKGTSTHYSLSLLLKNERLYPEQEVEQLSLFDADSSEVKRREADLVRMLGEYCEHV
                     TKIRFLIVDYVGQEAVSASLEDYTPQFLSVSSVDISHLLPSSAGIIRTEAIRDEQVND
                     LVDEPQLVTLKPIVEGIL"
     gene            17859..19016
                     /locus_tag="ACHU66_000893"
     CDS             17859..19016
                     /locus_tag="ACHU66_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR3389189.1"
                     /translation="MAQEFNGDRLRESRYFRHYSISQLAENVGVSKQMISKYEKNLSK
                     PSSEVLQKIIFELKFPLSFYQTKDKFITTDLGTFYRSKLTSSQTEKKSSEMLKKALAL
                     LANFFEDYVDFPKLAEFESHENASPEEVANQIRKNWGLGDSPISSVLRLLETNGFHIA
                     IINSKSEKVDAFGSFVELKMETDSERYYCILIDQDNNNFFRQQFSLAHELGHWALHAR
                     TVYPQELSNIEYRQMEQEANRFASSFLLPASAFSEDLKGHEEDLAYYLKLKSKWNVSI
                     ASMIYRAKDLGLLNSEQYTRLQKRMSARGWRREEPEDASIPVSRPILAKQAYKLLTDA
                     GLFEKQSIVQQFNLKYGYPLPIEIFSELMDIPKETLLQERNNIVHLKSRTL"
     gene            19061..20494
                     /locus_tag="ACHU66_000894"
     CDS             19061..20494
                     /locus_tag="ACHU66_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR3389190.1"
                     /translation="MNQNLSHYQFSPESQYLDRKSARKKPSELLKHLIAFANADGGQL
                     VIGIEDEKQENIITGFKDGRAYPIDDFKKIDREMRETPLDLSFEEIPVINHKGEEDFI
                     LVISVELSSNRVIAAPNDEVYLRQGDETVKLSYEQRTQLSYDKGQRFFEDEVVPDASL
                     EDIDDSLVQDFKNRFDISERSVEEILKARRFLINGKLTKAAILLFGKYPSAFFPQARV
                     RFQRFDGTDMGTGSSFNVIKEVTFDDALPILIIKVRDFIRTQLREFQYLDDSGQFKIL
                     PEYPEFAWFEGVVNAVTHRDYSVYGDHIRVLMFDDRLEIHSPGKLPNIVTVDNIKHER
                     FSRNPRIARTLTEFGWVREMNEGVKRIYSEMESAFLHEPKFSEPGNKVVLTLENNIVS
                     RHLRTRDSLEKQFTDFGDLNADEQLLVHYMYNSGEKMTTAKAIELTGRSRSFVVKMLH
                     HLRDLEIITWFGSSKNDRNQYYLLIDK"
BASE COUNT         5961 a         3937 c         4754 g         5857 t
ORIGIN      
        1 aaattcttta ttctgggaat ggcgttgttt gactactagc tctcagtttt atatttatct
       61 aattttatca gggatgtatt acttattgtt agcgctattg aatttttttg aagtcggctt
      121 cggtgagtat cttctaaaag gtcctgggtt ggatggcata gaaaccatta gtctactttc
      181 ttacctattg tttcaagcac agaatatttt tttgttgcaa cagctatttt ctggtttatc
      241 aacaacaaac ttcaacttaa cgatactaag aattgggagt ataaaagatt ggtttcttgt
      301 caagcttgtg tccttgatat ttctatcact tttattgggg ttgtttcaaa cagttcctct
      361 gttttttata ggtaatatgg actatttgtt tttcttgcat tttttcgtag ttaatagcac
      421 taattttatt ttgttcagtt cattgctctt tttattgttc cattttgatt tttcggatgc
      481 tgtctcgttt tgcattactt attctttact cgtttttatc attttttcag gagaaatttt
      541 aggagaaaat acaaattatt tcgttacgat attggcacgg gtagctgagt atgagaaaaa
      601 ttcaaatttg tcacttttca tcgctatatt ctttgagatt atactaagtg cggcgatact
      661 ttcttttata aagatcagat attctttggg aggtaaaata catgaataat agtattataa
      721 aaattgaaaa ccttagcaaa aaatttaaga attgtgattt atttgataat ttacactttg
      781 agtgttcgga aggggaaata gtagggataa ctggtataaa tggttcgggg aagagtgttc
      841 tgttcaaatt gatagcgggc cttatgcaac cgaattctgg gactatcaag gtgtatggag
      901 aagatattgt cggaaagatt ccatctggtt taggagcatt aattgaagaa ccaggcttta
      961 ttcccaatta tagtggattt gaaaatttag aatttttatc aactatccaa aatatcattg
     1021 gtaaggaaga aatacttgca accttacatc gtgtaggact ctttgaagat aaggataaaa
     1081 aggttaggaa ttactctctc ggtatgaaga aaaaattagg aattgcacag gctattatgg
     1141 aagagcctag aatccttcta ttggatgagc cgacaaatgc tttggatagt cagagcgttc
     1201 agagggtgta tgacttgcta aaagaacttt caaaagaaaa gggtgttacg gttttattgg
     1261 ccagtcataa tgatagtgat attagacatc tatgtgataa agtttatctt ctcgaaaata
     1321 agcaatgtaa gttattgaaa tagaaataat ttatgaattg tgcattcagt tttgtacaaa
     1381 attcgtgact attttgacgg tacggctaat ttgtaaaggt gaatttgaaa aagaatagag
     1441 aaagtctact cggtttattg gaagataata tcagttgaca aaagagttat tccttgatag
     1501 aataaatgag gtccaaaaag gacaaatcta gctttttctt ggttacaggt cgccaacctg
     1561 tcactcggat taggctcaaa acaaaggaga acatatcatg gcaatctcaa aagagaaaaa
     1621 aaatgaaatc atggcacaat atgctcgtca tgaaggcgac acaggttcag ttgaagtaca
     1681 agtagcagta cttacttggg aaatcaacca ccttaacgac cacatcaaac aacacaaaaa
     1741 agaccacgca acttaccgtg gtttgatgaa aaaaatcggt cgccgtcgta acttgttggc
     1801 atacctacgc cgcacagacg taaaccgtta ccgtgaattg attcactcac tcggactccg
     1861 tcgttaatat aggaaactgc catgtcttcg gacattggca gtttttctta tgttccatta
     1921 tggaagtgta gtgctcggaa aaagctgggt gttcttcgtt tgattgaaga gtatgtgatt
     1981 cgttcggttg tttatatagc aggttagatt ttccctcaaa ttccttcctc aattccccac
     2041 attttccgtc aaatatgata aaataagaag tatgcgtggt tttgtgcaaa agagagtgaa
     2101 acaagcttgt ttgattgtct tttgtagaaa atacgcagga aaaatttcag aaaaaatagg
     2161 agacaattca tgtcaaaaca agtatttgaa acggtttttg ctggcaagaa attggtcgtt
     2221 gaaacaggcc aggttgccaa acaagccaat ggtgcggttg ttgttcgcta tggcgactca
     2281 acagtcctta cagcggcagt tatgtccaag aaaatggcaa cgggtgattt cttcccgctc
     2341 caagtcaact atgaagagaa aatgtacgct gcagggaaat tcccaggtgg ctggatgaag
     2401 cgtgaaggcc gtccgtcaac cgatgcgacc ttgacagctc gtttgattga ccgtccgatc
     2461 cgtccaatgt ttgcggaagg cttccgtaac gaagtccaag tcatcaacac agtcctttct
     2521 tacgatccag atgcctcagc tccaatggca gccatgtttg gttcttcatt ggcattggca
     2581 atctcagaca tcccattcaa cggcccaatc gcaggtgtcc aggttggtta tgtcaatggt
     2641 gaactcatca tcaaccctga caaggctcaa atggaagagt ctctccttga cttgacagta
     2701 gctggtaaca aggatgctat caacatggta gagtctggtg ccaaagaatt gtcagaagac
     2761 atcatgttgg aggcactcct caaaggtcat gaggctattc aagaactcct tgacttccaa
     2821 aatcaaatcg tagcagcagt cggcaaggaa aaagcagatg tggaacttct tcaagtggac
     2881 ccagaattgc aggctgagat tgtcgcagct tacaatgacg atttgaaaaa agcggtgcag
     2941 gtagaagaaa aattggcccg tgaagatgcg accaatgccg tgcgtgagac cgtcatcgca
     3001 gcctacgaag aaaaatatgc tgaacacgaa gaatttgacc gtatcatgcg tgatgtccat
     3061 gaaatcttgg aactcatgga gcatgcagag gttcgtcgtt tgattacaga agacaaggtt
     3121 cgtccagacg gtcgtcgtgt ggatgaaatt cgtccgctgg acgcagaagt ggacttcttg
     3181 ccaaacgttc atggctcagg tctctttacc cgtggtcaaa ctcaggcact ttcagtcttg
     3241 accttggccc caatgggtga aactcaaatc atcgacggat tgggcgacga gtacaagaaa
     3301 cgcttcttgc accattacaa tttcccacaa tattcagttg gttcaactgg tcgctatggt
     3361 gcaccaggtc gtcgtgaaat tggtcacggt gctctcggtg agcgtgctct tgagcaagtc
     3421 ttgccaagct tggaagactt cccttacgct atccgcttgg tagcagaagt tttggaatca
     3481 aacggttctt catcacaggc ttctatcaca gctggtaccc ttgcccttat ggcaggtggt
     3541 gtgccaatca aggcaccagt tgcagggatt gccatgggtc tgatttcaga cggtaccaac
     3601 tacaccgtct tgaccgatat tcaaggtctt gaggaccact tcggcgacat ggacttcaag
     3661 gtggctggta ctcgtgacgg tatcacagcc cttcaaatgg acatcaagat tgacggtatc
     3721 acaccgcaaa tcttggaaga agccttggca caggctaaga aggcacgttt tgaaatcctc
     3781 gatgtcatcg aagcgacgat cccagaagtt cgtcctgact tggcaccaac tgcaccgaag
     3841 attgacacta tcaagattga tgtagataag atcaagattg ttatcggtaa gggtggcgaa
     3901 accatcgata agattattgc agaaactggc gtgaaaatcg atattgacga agacggtctt
     3961 gtggcgatct tctcaccaga tcgcgatgcg attgagcgga ccaaggaaat catcgcaggt
     4021 cttgttcgtg aggcaaaagt ggacgaagtc tttcaggcaa aagttgttcg tttggagaaa
     4081 ttcggtgcct ttgttaacct ctttgacaag acagatgccc ttgttcacgt ttctgaaatg
     4141 gcttggactc gtgtcaataa accagaagac ctagttgaaa tcggcgatat tgtcgatgtt
     4201 aaagtcatca agattgatga taaaggacgt attgacgctt ctatgaaggc tcttcttcca
     4261 aaaccagaag gttatgtgga gccagaaaaa cgcgagcgtt ctgacagacc acgccgtcat
     4321 aaagacaaca aagagaaaaa agataacaac tttggtgaat tcaaattcca caaggttgaa
     4381 aagaaataag ctgagtagac aagttcggta gcgggcttgt cttctcgcta gaaggagtga
     4441 ctatgggttg gtggaaggac accattgaga ttgtaaaaga aaaagatccg gcagcacgga
     4501 cgaccttgga agttcttttg acctacccag gtgtcaaggc cttggctgcc caccgtctgt
     4561 cccattggat gtggaaaaaa ggctttaaat tactagctag gatgcacagc caattttggc
     4621 gtttttggac taatattgaa attcatccag gggcggatat tgcttcaggt gtcttcatcg
     4681 accatggagc tggtttggtt atcggagaaa ccgccattat tgaatcaggt gtcatgcttt
     4741 accacggagt tacgcttggt ggaacgggca aggatacagg caagcgccac ccgactgtgc
     4801 gaaaaggggc tctggtttct gcccacgctc aagtcattgg tcccattgag attggggaaa
     4861 atgccaaagt tggtgcagct gccgttgtcc tagccgatgt tccagcagat gtgacagtgg
     4921 tcggtatgcc agccaaaatc gtccgtgtac acggtcaaaa agatgagaag gtcattcatg
     4981 acatggaaga tggtcgtgaa cactacacga ctaaattagc agagctgaga gaagctagtc
     5041 accgttcatc gcacttgtag gaaagaaggt tgacgtgcta ctagaagaat ttgaaaatac
     5101 ctctgcagtc attgaaccga cggatatggc cattcgtggt ggcggagagg tttgtgagac
     5161 catgattttc tccttcaatg gggaaattgt ggaccgtgtc cgtcagttac ctgagagcag
     5221 agaaggtggc tatttagaaa gcattaatgg tcgtcatcct tggtatattt tggaaagaga
     5281 tggggtgcga gttgcggtca tgttggcagt aatcggtgct cccatggcag ttggtcactt
     5341 ggaggaactg aaagcctgtg gctttgagaa ttttatcgtt cttggctctt gtggggtgct
     5401 ggataagtcg cttgttgcgg ataagattat ccttcctagc tctgccctgc gggacgaggg
     5461 aactagttac cactacgctc ctgccagtga tgaaatcagc tatgaccctg ctctgctatt
     5521 aaccatggaa aaggccttgg atcaagcggg aatcgagcac gtgcgggcca agacctggac
     5581 gacggacgct ttctatcggg aaacgtctgc caaggtcaaa cgtcgcctag ccgctggtgc
     5641 tatggtggtc gatatggaag cttctgctat catggcctgg gcaaactttc gtcaggccaa
     5701 ggtctatcaa tttttctaca cggcagacta tgtggactac cacaagaatg aatgggatgc
     5761 ccgtcgagag gaacggacgg ctgacagtat gaccttcttt gaagtggcca tggcgattgc
     5821 aagaaaatta gaaagtacat catgttaaaa aaattagtaa gacaaaactg gccttatgtg
     5881 ttgaccgcca ttgctgggac aatcatgttt atccttaaat tctcccaagg aagctggcag
     5941 gtagggatga tttggttggc agcgacagct tattggctag taaaattgta tcaaaagtat
     6001 caagtcctaa agaatactca gaagtaatgg agaaactatg attaaaattt acgataccat
     6061 gactcgcagt ctgcgtgact ttgtgccctt ggaagagggt aaggtcaaga tgtatgtctg
     6121 cggtccgacg gtttacaact atatccatat cggtaatgcc cgtagtgtcg tggcttttga
     6181 taccatccgc cgctattttg aataccgtgg ctatgatgtg acctacattt caaactttac
     6241 ggatgtggat gataagatta tcaaggctgc taatgaagct ggcatgacaa caaaggaact
     6301 ttctgataaa tttattgcag ctttcaagga agatgtggca aaactgggtg tcaagcctgc
     6361 taccaaaaat cctcgtgtca tcgactacat gggtgaaatc atcgattttg tgcaggtctt
     6421 gattgacaag ggctacgcct atgaagcttc tggagatgtc tatttccgtg tggaaaaggc
     6481 ggagaattat gctcgtttgg ccaacaaaac cctgtctgac ttggaggctg gtgcttctgg
     6541 tcgagttgag ggagaaggtc aactgaaaga acatcctttc gactttgccc tgtggaaaac
     6601 tgccaagcca ggtgaagtgt cttgggaaag tccctggggg gcaggtcgtc caggttggca
     6661 tatcgagtgt tcggttatgg cgacagagat tttgggagct accattgaca ttcacggtgg
     6721 gggagcggac ttggaattcc ctcaccatac caatgaaatc gcccagtctg agtgcaaaac
     6781 aggtcaaacc tttgccaact actggatgca caatgccatg ctcaatatca acgatgagaa
     6841 gatgtccaag tccttgggca atttcctgac agcccatgac atgttggaac aaattgacgg
     6901 tcaggtcttg cgcttcttcc tatctaccca gcactaccgc cgtccactca actacactga
     6961 gaaggccatt tcagatgcgg aaatcaatct aaaatatctg aaaaatactt atacacaacc
     7021 tgtgcaaaac tctgtggata agtcagctct tgaattacat ctggcggcct ttgaagcggc
     7081 aatggatgat gatttcaatg cggcaaatgg gattacggct atctttgact ttgccaaatg
     7141 gatcaactca ggcaattacg atgaaactgt aaaagaagcc tttggcaaaa tgctagaagt
     7201 gttcggtatt gtctttgaag aagaagtatt ggacagcgaa attgaagcct tgatagcaga
     7261 acgtcaagca gcgcgtgcca atcgagactt tgcgacggct gaccgtattc gtgatgaatt
     7321 ggcagcccag ggcatcaagc tcttggatac caaggatggt gtgaggtgga cacgtgacta
     7381 aggcagtgga tgtcaatctc atcaacggaa ttgccctggc ttttgaaggg gatgctgtct
     7441 attccatgta tgtcagacgg cacttgattt tcaaggggct gaccaagcct aacaaattgc
     7501 atggcgaggc taacaaatac gtctcggcca aggctcaggc aggcttgatt tctgccctct
     7561 tagaagctca gctattgacc gaaaaagagg aagagatcta caagcggggg cgcaatacca
     7621 atagccacac caaggccaag aatgcggacg tggtcaccta tcggatgtcc acaggctttg
     7681 aggcggtctt gggatacctg cacatgacag agcagacgga gcgcctggat gaactggtag
     7741 gttggtgtat tgacaatata gaaagaggat aagatgaacg agaaggaact tgcaggttgg
     7801 tttccgcagg ctaagttatc gcaggaagcg gacgcacagt cgcttgcttt aggcgacggt
     7861 cgctatctga atctggcgga tgcggatttg acagagcgag aacgctatct cttgtccttg
     7921 ctgtcccatc ctcaagagca gaaaccgtca agcccttggc agtcctatct acatggacag
     7981 agctctctgc cccaggcggt tgaagctatt caatttatcc atgtccacct ttggtcgcag
     8041 aaggacgccc agttggatgc atggcagacc atgatgaccg acctcctacc cagtcaattg
     8101 gctcaggtac aactgtcgag tcaggattac ctttttatca tcgaacaaag tcgttggacg
     8161 gactatcaaa ccattctggc agacacccta tcagccttgg agtttgactt tggcttgcgg
     8221 ttgacattcc tagtcggtca ggtttggtct cgtcaacttg cagatcgttg gtctcaactc
     8281 tatcaagcag aagcagccct ctttgctcac tggcgagcgc actaccatca atcaaccgtc
     8341 ctatccttta gtcaactctt tctttgggga caaggcagag caggtttgga gtttggtatg
     8401 ataaaagcta gtatgctaca gttgattgac cagcaagata tgcaggacat cattctagcc
     8461 ctgtggacag aaggagccgt ggtcaccaag accgctcaga tcctctatat ccatcgtaat
     8521 accctccaat accgattgga caagtggcag gatttgaccg ggctccagct caaagacctg
     8581 actgatttaa gtttttgtta tcaagtatta ctagataggc ttttttaatc ttttgtgcaa
     8641 cttgtacaaa agattgtgtt ctttttgttc accttgacct agaaaaataa aacgctttca
     8701 tttataataa aactatcaaa gaaagtgagg cttgaacatg gttcaactta atttaaaaaa
     8761 tatttataaa aaatatccaa atagcgagca ttattccgtt gaaaacttta atctagatat
     8821 taaagataaa gagtttattg ttttcgtggg cccatcagga tgcggtaaat caactactct
     8881 tcgtatgatt gctggattgg aagatattac agaaggtgaa gcttacatcg acggtgtgtt
     8941 gatgaacgat gtagcaccaa aagaccgtga cattgccatg gtattccaga actatgccct
     9001 ttacccacac atgactgtat ttgacaacat ggcttttggt ctgaaattgc gtaaatacag
     9061 caaggatgaa atcaaaaaac gtgtagaaga agcagcacaa attctaggct tgactgaatt
     9121 cttgcaacgt aaacctgctg acctttctgg tggtcaacgc caacgtgttg ccatgggccg
     9181 tgccattgtc cgcgatgcaa aagtattctt gatggatgag cctttgtcaa acttggatgc
     9241 caaactgcgt gtatccatgc gtacagagat tgctaaaatc caccgtcgta ttggtgcaac
     9301 aacgatctac gtaacgcacg accaaacaga agccatgacc ttggctgacc gtattgttat
     9361 catgtctgcg actaagaatg aagcaggtac aggtacaatc ggtcgtatcg aacaaattgg
     9421 tagcccagaa gagctttaca ataaacctgt aaacaaattt gttgccagct tcatcggtag
     9481 ccctgcaatg aacttcttta cagtgactct tcaagagggc gtgcttgttg gagatggttt
     9541 caaagtagat cttccagaag gtcgtcgcaa gcatttggaa gaaaaaggct acaacggcaa
     9601 atcatttaca ttgggtatcc gtccggaaga catcaaagct tcacaattgg agttggacac
     9661 ttatccatct tctatcgtgg aagcagaagt tgtcgtttca gagcttcttg gtgcggaaac
     9721 catgctctat tctaaagtag ggaatacaga atttgtatct cgtgtagatg cgcgtgatta
     9781 ccacaatcca ggagacaagg ttcgcttgac cttcaacctc aacaaagctc atttctttga
     9841 tgatgaaaca gatcaagcca ttgtttaatc ttttataatt tgttcataaa acaccaaaga
     9901 cccgctatta tgacgggtct ttttcatctt tatagacttc ataaaaatca accttgaggc
     9961 gaataaaggc ttccatatca tgcaggagtt ggaagagggt ggcacgggtt tcaaattctt
    10021 cccttgtttg tggcagaggt cgctcgcgga aggtggcatg gaagagctcg atgtctagga
    10081 gcagttcctt ggcggagttt tctcgactca gttgctgggc tgctcgctcg aagaggctgg
    10141 acaggatgac attttctctg ccttccagta aacatttgtt gatatttcct gccatggtcc
    10201 gcaggatttt attttgtgcg gcccgcattt caaagtaatg gacctggtaa ttggtttgct
    10261 ggaagagttg gttgtggcgg tccagataga cgactttcag agcttgctcc aaggtctgat
    10321 ccaactgcgt aatcaattca gcctcgtttc gtccgtctcc gttaagtaga aaggcttcaa
    10381 agcgaagcag aatctgcttg agcaggtctt cgacttggtc gtggtaggcc tggattttct
    10441 tttcttgcga gggcatatag aggttaaaca ggagtgccag tcctgcccca atcagaaaaa
    10501 gcgccagctc atttcccaaa aaaatcagcg aaatatcttg ctccagcaag aggtgggtaa
    10561 cgaggacggt cgagggggcg atgccagcct cccagttgaa acggtaggcc agggggacgt
    10621 agagggctag gtaaatgccc agggtccaga tgccaaagcc aaagagggcg aaggtcagga
    10681 cggctatggt cagggccagg agggtggaaa agaggcggtt gcgagccatt ttgaagctgg
    10741 acttgcgggt gtcgaggacg ctgagaatgg cgatgatgcc tgccgctgtc gtataagaca
    10801 ggcccagtac ggtcgctagg taaatagcca gaacggtggc aaatatcagt ttgatggtgc
    10861 ggagggagag ggacatgagg gctcctttgg ttttagtcta tttctattgt atcataaaat
    10921 aaaaacctcc taaggatagg agggaattac ttatgcaaat agagccagaa taccgactac
    10981 taggtagcag actagtttgt agctttcgtt gatgaggata aggttgatag ggcgttgctc
    11041 aaagacgtag tttttcaatg aagcgaggac cgcaattacg cgcccatgcc accagctgca
    11101 tgaagtggtg aaacaccgag ggtcttgatg aagaaaatag ccacaacagc agtcagtact
    11161 tcaatgacaa gggaaatcac catttccttc tgtccatttt tgccagcctg acgatctgct
    11221 tctaccttag ccatgtcaat gcctgaagcc ttgatccaag catcgcggaa aatcaaacca
    11281 taccacaaac caccaatagc aaaggcaatc agccccgcca caattccaaa aataagtgtc
    11341 ataaaatctc ctttttcgat tccgtttgta cggacaaatt gatagaacta aggataacat
    11401 agattgtata ggcggtcaag tttttcagct ggaacacagt gagcgcctat tttcagtcaa
    11461 cctatgctat aataaaacca tggaacataa tgatatcgta tatggcgtac atgctgtagt
    11521 ggagagtttg gaggccaaca ccggcaacaa actttacatt caggatgatt tgcgcggaaa
    11581 aaatgtagaa aaaatcaagg cgctggcggc agagaagaag gtgtcgattt cttggacgcc
    11641 caagaagacc ctgtctgaca tgacagaagg tgccgttcac caaggctttg tcctgcgggt
    11701 ctctgagttc gcctatgcgg acctgtcggt gattttggaa aaggcagagc aggaagacaa
    11761 tcccctcatt ctcatcttgg acggtctgac agacccccac aatttcggct cgattttgcg
    11821 gaccgcagat gccactcagg tagctggcat catcattccc aagcaccgag cagttggtgt
    11881 gacgcccgtt gtggcaaaga cctcaacagg tgcggtggcc catgtcccaa ttgcccgagt
    11941 gaccaatctt agccaaaccc tcgacaaact caaggaggct ggtttctggg tatttggtac
    12001 cgatatgaac ggaaccccaa gccacaagtg gaatacggct ggcaagctag ctctcattat
    12061 cggcaacgaa ggcaagggca tctccagcaa tatcaaaaag caggtggatg agatgattac
    12121 cattcctatg aagggccatg tccaatctct caatgctagc gtagcagcag ctgtactgat
    12181 gtacgaagta tttcggaaga aaatctaaat gaaagaaaaa gttctcattg tagatggcta
    12241 caacatgatt gccttttggc aggccaccaa gcaagatttt aaaaagggag acttggatgc
    12301 ggccagaacg accttgcttc gcaccttgtc tcactacgca gcctttgaac agattgaggt
    12361 aatttgtgtc tttgatgccc accatgtgcc aggtcttcgc cagcagtatg acgaattcaa
    12421 ggtatcggtc atttttacag aggaggaaga gacggcagat agttatatcg agcggctcag
    12481 cgcccagctc aatagtgacc gccgcaagca ggtttccgtc gcaaccagcg acctcaacga
    12541 acaatgggtg gtcttttcac aaggggcctt acgggtttcg gcgcgtgaat tagaggagag
    12601 aaccaggatt atcaagaagg acttggacaa gtttgtggac caggtagaac tctacacccc
    12661 acgactcaat ccctggtcag atgggaattt tcaagcctta aaagaaatga tggaggaaat
    12721 gaaagagtga catttacaat cgtaaccgat tcgacatcgg atttgccaat cagctgggtc
    12781 caagaaaacg atgtgaccgt gcttggtttg accattaacc tagacggcgt gacttatgaa
    12841 acagtcggtg aaaaccgctt gacctcagca gatttactgg agaaaatggc ttctggcagc
    12901 caacctacga ccagtcaggt caatgttggt cagtttgaag aagtttttga agaggcagcc
    12961 aaggaaggtc atgaggtact ttatctcgct ttctcagcag ctctttcagg gacctaccag
    13021 agtgcagtca ttgcacgaga gatggtagtg gaccaatatc cagatgcagt catcaccatc
    13081 gttgatacaa aggcagcgac cattggagaa ggctacttag tcatgaaagc cgttgaggca
    13141 cgtgctgcag gtaagactct ggcagaaaca aaggcaatcg tagaagattt ggtgcctcgc
    13201 ttacggactt acctcttggt cgacgacctt aatcatttgg tgcgaggtgg acgcctgtct
    13261 aaagcggcag ccctcatcgg tggattggtc aatatcaagc ctctccttgc actcaatgcg
    13321 gaagggaagt tagaagccat tgccaaaatc cgtggtcgta aaaaaggaat taaggaaatg
    13381 ctgaatctga ccttggataa cttggatcat tctacggtca tggtggccta tacgggtgat
    13441 attgaggcgg cagagggcgt caaatctagt ttattggaag acgaccgtat ttctgatgtt
    13501 ctattgacag aattaggacc agttattgcc actcatacag ggactggagt cttagctatt
    13561 ttatcaattg ggacagcaat gagagcctag attctaaggg atatttcttg taaagatgtg
    13621 tctcttttgt acgatgacga cttatgtagc atagggtaat caaaaggaga tacgataatg
    13681 ctcattgtca agttgttccg accagaagcc ttggatggct gaaactggtg ttttacaggt
    13741 cttctggtct atacggtgga tgaattggtc ttggaagaat ttgataaaac ctttccttat
    13801 aaggagaagc tggtcttgcc tggacaacta gggcagtagg gaaagtaaac ctgataaggc
    13861 tttcattctc agcagctctg ctatctttca aaaaataaaa aaagtaaagt aaaatagctt
    13921 gacggagact agtagatttg ataaaatggt agacggtatt gtttacccca tttgaaaggc
    13981 cccggaacct tccaaatacc cgcggaccgg aacatccgcc cagtaaacaa aaacgattcg
    14041 tataggagaa atcatgaaca aaacaacatt tatggctaaa ccaggccaag ttgaacgtaa
    14101 atggtatgta gtagacgcta ctgatgtacc tcttggacgc ctttcagcag tagttgctag
    14161 cgttcttcgc ggtaaaaaca aaccaacttt cacacctcac actgatactg gtgacttcgt
    14221 tatcgttatc aacgctgaga aagtgaaatt gactggtaaa aaagcaactg ataagattta
    14281 ttacactcac tcaaaccacc caggcggatt gaaatcaatc tcagctggtg aattgcgttc
    14341 taaaaacgct gttcgtttga ttgaaaaatc agttaaaggt atgcttccac acaacacact
    14401 tggtcgtgca caaggcatga aattgaaagt gtttgtgggt ggcgagcaca ctcacgctgc
    14461 acaacaacca gaagtacttg atatttcagg tcttatctaa gggaaaggac gtaatctaaa
    14521 tggcacaagc acaatacaca ggtactggtc gtcgtaaaaa cgcggttgca cgcgtacgtt
    14581 tggtcccagg tactggtaaa atcacagtaa acaaaaaaga tgtagaagag tacatcccac
    14641 acgctgacct tcgtttggtt atcaaccaac cattcgcagt aacttcaact caaggttcat
    14701 acgacgtttt cgttaacgtg aacggtggtg gttacggtgg tcaatcaggt gcgatccgtc
    14761 acggtatcgc gcgtgcattg cttcaagtag acccagactt ccgcgattca ttgaaacgcg
    14821 caggccttct tacacgtgac gctcgtatgg ttgaacgtaa gaaaccaggt cttaagaaag
    14881 cacgtaaagc tagccagttc tctaaacgtt aattatcagc gatatatcaa cgtttcaaag
    14941 cactcgcagg gttttcccta cgggtgcttt tttctgtgct ttttacaaaa gtttgcccta
    15001 aaatttgccc taaatttgca ctactcattt ttaagatgtt gataggcgac atgaccagca
    15061 gatagaggga caatattaga agtgttgaca taggtcttag ttgtgagagt atcactatga
    15121 gttaatgctt ctgagatagc ttctaagctc attccagctt tcttagcaag tgttgctcca
    15181 gtgtgtcgga gtttatgagg tgtggcatgt gttagcttag gatgtcgctt cttaatggtc
    15241 ttcattttat tattgagata gtcagcatgc aaaggcttat tgatgtttcc tttggtatca
    15301 atataagtga agacgaactg ttcaggattc gtaataattc caaactttgc aagttcctgt
    15361 ttttgttgtg atttccaacc ttggagaagt tgaagcaagt cagttgaaat agcaaagatt
    15421 gttttcttgt tccctttggt tgatttcaca tctccatacc tatctagtgc ttggagtaat
    15481 tgaatttggg atttttcaaa atcaatgtgt ttccactgca aagcataaga ttcagatttt
    15541 cggtcgccta aaaagaaggt aagataaaat agaacatagt ccttaagtga aatttttccg
    15601 ctagccaaat catcttggaa ggctgagaac cattcctgta attgctcttg tgttaggtag
    15661 aggtcttcat ctcgcctagc ttctgccaac tggatttttt tagttgattt gatacgacgt
    15721 aaggttttag cgattttatt tgattctatg tactctaatt cttctgccca atcaaagatg
    15781 gatatgacat agctacgtaa agttttgaag ttcgcatatt cacttgcttt tgctgccatt
    15841 aaatttagaa taacctgctt gttctgattg agaaattgaa cagtataatt acctaacata
    15901 ggtaaaatat gttttctgaa acaagttttt gtattagcta ccgttgattt gctaggtggt
    15961 ttagttgttg aagtagtctg tcctgctttg taggcttccc accaaataga ttcgtagaaa
    16021 tctttgaata gaatgtctcc cttacctatc cctgtaaact cgttgttctc aatctgattg
    16081 atttttgtta aaaggtctat ctctgcttgc cgtgcctcct ttctggtctt gaagcgctta
    16141 tcaaaatagt tattattgac gataccaagt ggcatccgtg tttccgttgg aatatacact
    16201 tttaagcgat atgttccatt ttttgtctta gtgatagtca tgatattctc ctttatgtcg
    16261 aaccagaaaa tatcatgacc attataaaac aaaaatgtgg atatttatag ttttgtgaaa
    16321 gtagccatga gtcgatggct tgtttgctga aacgaatcgt ttttccgatc ttaatagatg
    16381 gaagtccttt gcgaatccat tcatccaacg tgttatttga aattccaagg tagtcacatg
    16441 cttgtttttt gtttaagtat gaattgtcta tatttcgatg atgaatttgt ttttcaattt
    16501 ctgatgtaat taaacttgaa attaatatct gaatttgttt gatttgatcg tctggtaaga
    16561 taacttgcat tttaacctct tttctcgtaa aatttgcaat tgttttttga cattagttat
    16621 tatgccatag tgtggactaa aatcagttga ttttttaaaa tacagaataa tatttcttgt
    16681 atgtcttccg tttgggaaga caatttttaa aagaaacgct gttgtatcaa catttttgat
    16741 attttggaaa gttgtaaaat catattaaaa accgttaaat aattctttta aattcaacaa
    16801 tcgatttaaa attaattcat ataaatcaat tttataatga tgttttctac gtaacttatt
    16861 gttatcttaa tttagaggtg gagtaatctc ggattgaaca actattggta atatttatta
    16921 ggatagcaaa tagcttgctg tcctaatttt tttattatag catttagtaa ctagctattt
    16981 aatctactta gatgtgagta tgtgtaagga gaaagatatt agcatcgctg aggagatggg
    17041 attatttaat tctacacact ttggttgact tttttggtag tttttcttgt ttttgtctcc
    17101 ttggtctgtt ataatgaagt taaataacaa acgaattgag gtgaataaat gaaatatagt
    17161 tatgatattg aaagagaagt aatcatcaag tgtttggaag cgatgagttt tacaactagg
    17221 catctggagc aggattataa agttgctatc tcagagacta gtcaaaatgg aatttatcag
    17281 gctacttggg cacgaagatc gaatatgctt aaagacttat ttgataatag tgttgtttgg
    17341 aaagttttgc atattaagag ggggatttgg caatttgacc ctatactgaa tattgagaca
    17401 ggtgaactaa tagccttctt ctccaaaaat aattttagga caattcgtaa tcgatatttt
    17461 aaaaaaggaa cctctactca ttattcctta agtttactct tgaaaaatga gagactttac
    17521 cctgaacaag aagtagaaca gctttctctt tttgatgcgg atagctctga agtgaaaaga
    17581 agagaagctg atttggttag gatgcttggt gagtattgtg aacatgtgac taagattaga
    17641 tttttgattg ttgattatgt aggtcaagaa gctgtgagtg cttcgttaga agactacact
    17701 ccacaatttt tatcagttag ttcagtggat atttcgcact tacttccaag ttcagctggt
    17761 ataattagaa ctgaggccat acgtgatgaa caggtgaatg atttagtaga tgagcctcaa
    17821 ctcgttacat taaaaccaat agtagaagga atattataat ggctcaagaa tttaatggag
    17881 atagacttcg agaatcgaga tattttagac attattccat tagccaattg gcggaaaacg
    17941 ttggagtttc taaacagatg atttctaaat acgaaaagaa tttatcaaag ccgagttctg
    18001 aggtattgca aaaaataatt tttgaactga aatttccgct gtccttctat caaacaaaag
    18061 ataaatttat aactactgat ttaggtactt tttatagaag taagttaaca tcctcacaaa
    18121 ctgagaagaa gtctagtgag atgctaaaaa aagcactggc tctcttggcg aatttttttg
    18181 aagattatgt tgatttcccg aaattagcag aatttgaaag tcatgaaaat gcttcaccag
    18241 aggaagttgc aaatcagata agaaagaatt ggggattagg tgatagccca atttccagtg
    18301 ttcttaggtt actagaaacg aatggattcc atattgctat aattaattca aagtctgaga
    18361 aagtggatgc ttttggaagt tttgttgaat tgaaaatgga aacagattct gagagatatt
    18421 attgtatttt aattgaccaa gataataata attttttcag gcaacaattt agcttggctc
    18481 atgagttagg gcattgggct ttgcatgcaa gaacagtata tccacaggag ctttcaaata
    18541 ttgaatatag acaaatggag caagaagcta atagatttgc ttcgagtttt ttattaccag
    18601 cctctgcttt ttctgaagat ttaaaaggac atgaagaaga tttagcatat tatttgaaat
    18661 tgaagtctaa gtggaatgta tctatagcca gtatgattta tcgtgcaaag gatttgggtt
    18721 tgttaaactc ggaacaatat actcgtctac aaaaaagaat gagtgccaga ggatggcgaa
    18781 gagaagagcc agaggatgca agtattccag tatcacgacc gatccttgct aaacaggcat
    18841 ataaattact gacggatgcg ggcttatttg aaaaacaatc catagtccag caatttaatc
    18901 tcaagtacgg ctatcctctt ccaatagaga tattttcaga gcttatggac attccaaagg
    18961 agacgttgtt acaagagaga aataatattg tacatttaaa atctaggact ttgtaaatca
    19021 tgaattgtta agtatttttt atatatttaa ggagaactat atgaatcaaa acttatctca
    19081 ctatcaattt tctccagaaa gccaatacct agatcgaaaa tcagccagaa agaaaccgtc
    19141 tgaacttctc aaacacttga ttgcttttgc caatgcagat ggcggtcaac tagttattgg
    19201 tattgaggac gagaaacagg aaaatattat cactggtttc aaggacggca gggcttatcc
    19261 catagacgat ttcaaaaaga ttgatcgtga gatgcgtgag actcctttgg atttatcttt
    19321 tgaggaaatc cctgtaatca atcataaggg tgaagaagat ttcattttag ttatctcagt
    19381 tgaattgtcg tcaaaccgag tgattgccgc gccaaatgat gaggtttatc ttcgtcaggg
    19441 tgatgaaaca gtcaagttga gctacgagca acgcactcag ctcagctatg acaagggaca
    19501 acgtttcttt gaagatgaag tggtgccaga cgccagtctg gaagatattg atgatagctt
    19561 ggttcaggat tttaaaaatc gttttgatat atcagagcgt tctgtggaag aaatccttaa
    19621 agcacgtcga tttctaatca atgggaaatt aaccaaggca gcaattttac tctttggcaa
    19681 atatccgtca gcattcttcc ctcaagcgcg tgtccgattc cagcgatttg atggtacaga
    19741 tatgggaact ggaagtagtt ttaatgttat caaggaagtg acctttgatg atgcgctacc
    19801 gatcttaatt atcaaggtta gagattttat tcgcacccag cttcgggaat ttcagtattt
    19861 ggatgacagt gggcaattca aaattttacc tgagtatcct gaatttgctt ggtttgaagg
    19921 tgttgtcaat gctgtaacgc accgtgatta ttccgtctat ggcgatcaca ttcgtgtgct
    19981 tatgtttgat gatcgactag agattcatag cccagggaaa ttacccaata tcgtcaccgt
    20041 tgacaatatc aagcatgaac gtttttcacg aaaccctcgc attgccagaa ccttgacaga
    20101 gttcggttgg gtacgtgaga tgaatgaagg agtaaagcgt atctattctg agatggaatc
    20161 tgcttttctc catgaaccta aattctcaga acctgggaat aaggttgtcc tgacgcttga
    20221 aaataatatt gtcagccgac acctacggac tcgagatagt ttggagaaac agtttactga
    20281 ttttggagac ctcaatgctg atgaacaatt gctggttcac tatatgtaca attctggtga
    20341 gaagatgacc accgcaaaag ccattgagct gacgggaaga agtcgctcgt ttgttgttaa
    20401 aatgttgcat catcttcgtg atttagaaat catcacttgg tttggatcaa gtaaaaatga
    20461 ccgcaatcaa tattatcttt taattgataa atgattacag tcagggtac
//
LOCUS       DAZQCJ010000026        19938 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000026 DAZQCJ010000000
VERSION     DAZQCJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19938
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.026"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(33..1055)
                     /locus_tag="ACHU66_000895"
     CDS             complement(33..1055)
                     /locus_tag="ACHU66_000895"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFR3389191.1"
                     /translation="MTVNIDWEKLGFSYMKLPYRFIAHFKNGQWSEGQLTEDAELHIS
                     ESSPALHYGQQAFEGLKAYRTKEGKLQLFRPDQNAERLQRTADRLLMEPVPTDLFIKA
                     CKEVVKANADYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTVFAMPVGNYFKGG
                     LAPTNFLIQDKYDRAAPNGTGAAKVGGNYAASLLPGQYAKKQGFSDVIYLDPATHTKI
                     EEVGSANFFGITADNEFVTPISPSILPSITKYSLLYLAEHRLGMKAVEREVLVEELDQ
                     FVEAGACGTAAVISPIGGIQIADKLHVLHSETEVGPVTRKLYEELTGIQFGDIEAPEG
                     WIVEVD"
     gene            complement(1166..1783)
                     /locus_tag="ACHU66_000896"
     CDS             complement(1166..1783)
                     /locus_tag="ACHU66_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HFR3389192.1"
                     /translation="MKIIKLFFSIKVYLWLVLSFLLLLCGVWGYGYISGEKSNQQEVS
                     IHTAIESVEQVNELVFLNTAVQKIVTAKNYTVLFGQEMPFSEKSALLIIKYKAKFGIK
                     SPVSIEHLSENRYKITLPAFKVIGLELDAEEPYTLYDKSGEILSFATEEIDTAQLITD
                     EFQSAEQETFLADYQDDIKESAKTYYQNLFSAISPEIQLEFVFKE"
     gene            complement(1955..4408)
                     /gene="parC"
                     /locus_tag="ACHU66_000897"
     CDS             complement(1955..4408)
                     /gene="parC"
                     /locus_tag="ACHU66_000897"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR3389193.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFDKGYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQDWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEMAGYLLADIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGATGISAGYATDIPPHNLAEVIDAVVYMIDHPTAKLEKLMEFLPGPDFPTGAI
                     IQGADEIKKAYETGKGRVVVRSRCEIEQLKAGKKQIVITEIPYEVNKAVLVKKIDDVR
                     VNNKVPGIAEVRDESDRTGLRIAIELKKDSDEQTILNYLYKYTDLQINYNFNMVAIDN
                     FTPRQVGLQKILSSYIAHRREIIIARSKFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFSEEQAEAIVTLQLYRLTNTDIVTLENEEAALREQIQTLA
                     AIIGDERTMFNLMKKELREVKKQFGNPRLSELQDQAETIEIDTASLIVEEETFVSVTK
                     AGYIKRTSPRSFNASTLEEMGKRDDDQLIFLQNAKTTQHLLLFTNLGNVIYRPVHELT
                     DIRWKDIGEHLSQTLMNFDTSEEIIFAELVENFEEGTYFAVTQYGQIKRVERKEFTPW
                     RTYKSKSTKYAKLKDAEDVVITVSPVVLDDIMLMTEKGYALRFNIEEVPIIGAKAAGV
                     KAVNLKDGDVVVAAFISNTSSVYLLTQRGSFKRMATEEIPVTSRAKRGLQVLRELKAK
                     PHRVFAAGPVLTDQGDFDLFSTANEDETTSQILHVQSKTGKLYEVDVTQLSLSERTSN
                     GSFISDTISDEEVFRAWID"
     gene            complement(4609..5061)
                     /locus_tag="ACHU66_000898"
     CDS             complement(4609..5061)
                     /locus_tag="ACHU66_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389194.1"
                     /translation="MIKFLRKKLTIEQLKKVPYASQYTEVLRSIWRADVPKYGISSTL
                     QGELLRQLEKLRWEAQANGNVNWCEEHSNYCRFIKETLYKGKVLSSQQKQELVLIMDY
                     LKSCGEYAQAYQENLIDDEELEIEKLAYVDDNLYDRVGDMIAFFYQRT"
     gene            complement(5064..5558)
                     /locus_tag="ACHU66_000899"
     CDS             complement(5064..5558)
                     /locus_tag="ACHU66_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HFR3389195.1"
                     /translation="MPKLTDRFQKRLSEWARKRKPFKNKQELEEKFDQALKIWQSSKT
                     RQHAENLLSEQGKLEHFLHSTERKLSRMPFGGDKFAAIPGLISMVRSYIRRDYSKLPK
                     GTILAIIGALIYFFSPIDALPDFILGAGLLDDAFVLNACLKLIKTDVDDFRSWQASQW
                     KVDE"
     gene            complement(5591..6193)
                     /gene="fic"
                     /locus_tag="ACHU66_000900"
     CDS             complement(5591..6193)
                     /gene="fic"
                     /locus_tag="ACHU66_000900"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFR3389196.1"
                     /translation="MVLENKLGIENSAELARLEEQISKKKAAQLFETGQLFQIEVGTF
                     AGLAHIHQTLFEEIYDFSGKIRDVNIAKDNFQFAPRIFLEQSLAYIDKLPHETFDEIV
                     EKYADMNIAHPFREGNGRSMRIWLDCMLRDSLGKVVDWNSIDKDEYFNAMVRSHVSTG
                     ELKYLLLQALTEDLGQATYFKGIDHSYYYEGYNLYRTEDL"
     gene            complement(6217..8166)
                     /gene="parE"
                     /locus_tag="ACHU66_000901"
     CDS             complement(6217..8166)
                     /gene="parE"
                     /locus_tag="ACHU66_000901"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR3389197.1"
                     /translation="MAKKEIDINNYNDDAIQVLEGLDAVRKRPGMYIGSTDGNGLHHM
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLSVSDRGRGMPVGMHATGKPTVEVIFTVL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFEQGGKPVTTLE
                     KIGTAPKSKTGTKVTFMPDDTIFSTTDFKFNTIAERLKESAFLLKQVTMTLTDERTGE
                     QEEYHYENGVQDFVSYLNEDKETLTPVLYFEGEDAGFQVQVAMQYNDGYSDNILSFVN
                     NVRTKDGGTHETGLKLAITKAMNDYARKTNLLKEKDKNLEGSDYREGLSAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVSDKLTFFLLENGELASNLVRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGSDFTLEDVNYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGRVYIALPPLYKMSKGKGKTEKIAYAWSDGELED
                     LRKDFGKGFILQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVSVLMGD
                     KVEPRRKWIEDNVKFTLEEATAFTK"
     gene            8309..8953
                     /gene="plsY"
                     /locus_tag="ACHU66_000902"
     CDS             8309..8953
                     /gene="plsY"
                     /locus_tag="ACHU66_000902"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR3389198.1"
                     /translation="MLVNLILLFIAYLLGSIPSGLWIGQTFFNINIREHGSGNTGTTN
                     TFRILGPKAGTIVFVIDFLKGTLAALLPLIFHAQGISPIVFGLMAVLGHTFPIFAQFK
                     GGKAVATSAGMLPGVAPAFCLYLVIIFVTSLYLTSMVSFSSVLAAALAILGALLFPAI
                     GIILPSYDWLFTVIIVFLGSFVIIRHKENIQRILKKEENLVPFGLNLTKQKKRA"
     gene            complement(8963..12616)
                     /gene="addA"
                     /locus_tag="ACHU66_000903"
     CDS             complement(8963..12616)
                     /gene="addA"
                     /locus_tag="ACHU66_000903"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFR3389199.1"
                     /translation="MAFEQFLSAEEIKAVQLAEANSDKQQKRTPEQIEAIYTHGQNVL
                     VSASAGSGKTFVMVQRILDKLKRGVGIDQLFISTFTVKAAGELKERIEKNLNETIAET
                     MDMDLRRHLSAQLADLTKADIGTMDSFTQKLVTTYGYSLGISPQFRILQDETEKASLK
                     KEVFDQLFADYLEQDENGAFRKLVRNFSGNRKDNGGFRQVVYQVHDFSQSTSSPTKWL
                     KEQAVQADLYSQERIELILEQGFKEKVLDKLYQAADFFRYHVEWGRNDFGSAKYFANV
                     EEVLDLLTGLDSLDQKDLMERVEKVLLINDQSRGKGLTNANRPKDEHLIAFKEEYNAG
                     KSQIISGLRDLGQEVYELTLLKDYQIQALPMLILLRDFVLDFSQAYLDVKIKEAAFEF
                     GDIGHFAIRILEENVDIRQFFQEKYHEVMVDEYQDNNHSQERMLDLLSNGHNRFMVGD
                     IKQSIYRFRQADPMIFQEKFELYQADSQAGKLILLKENFRSQIEVLEATNAIFTRLMD
                     RQVGEIKYDETHSLVAGSPGQKIAQPKHEMEYLIYDQQDSANSSTDAEEETPLTAGEI
                     EVVAKEIIRLHNEEGADFKDITLLVQKRTHNDLIISIFEKHGIPIVADGGAASYLQSL
                     EVMIMLDTLRVINNPLNDYALVALLKSPMFRFDEDELTRISLQAGTGFFYQKMEIAQQ
                     TNGQHPELMSGELKKKIKDFLSILENWRAFAKMHSIYDLIWKIFNEKFYYDYVGALPN
                     GSKRQANLYALGLRANQFEKTGYKGLSRFIVMIDRALANDKDLADVQEFLPQNAVQLM
                     TIHKSKGLEFKYVFLMNIDKRFNLEDHYQSVIISRKNGLGIQYLADMKDKVNSPLPQV
                     RVLMNTLPYQDNLQELKIANLSEQMRLLYVALTRAEKKLYLVGKGNADKLAKKYDGKK
                     ADGVLAQSTRESVATFQDWILAIDEAFSGEDLHFKKVFVTDEDLTEEKIGKLTLKSKL
                     EDASLKDIRQSEDIAQALDQLSSVQELNERYKVAIELPSLRTPSQIKKLYEPILEQEG
                     MEVMEKYQPKRTFNLPDFSKKPKITGAQVGSAVHELMQRLDLSWLVTEDTVREALEAV
                     HAEQAIKDKINVQMILDFFDTDLGREILANTDKLHREAPFASLQTDSVSQENFVLRGI
                     IDGYLLYDDHIVLFDYKTDKYDQPSQLSQRYQAQMQLYAEALKKAYKIDRVDCHLILL
                     GGERIEVVEVKVN"
     gene            complement(12616..15882)
                     /gene="rexB"
                     /locus_tag="ACHU66_000904"
     CDS             complement(12616..15882)
                     /gene="rexB"
                     /locus_tag="ACHU66_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HFR3389200.1"
                     /translation="MKLVYTDIRNPLTQYLTDTSAEFAKEAKRVFYIAPNSLSFEMER
                     KVLEYLPEQATFDIIVTRFGQLARYLMIDRKEAGQPLDDVGLAMIFFRVLSQFEDGDL
                     KVYGRLQRDFGFINQLVALYKELQRANMSILDLEAMDSPDKQADLVKIFLAVTDILSK
                     ESFEHQSKLAQLTGLVETGQLDEQLKNIVLVVDGFSRFSAEEEALVSALNERVSEILI
                     GVYASKKAVQVIYAEGNVYQANVDFLRQLSAQFQTKATYIGQEPVLDSIGKFSKNMEA
                     YYDYSGTMIDLTPADQEKIQLWEVVNQKEEVEQVATAIRQHIHQGARYKDILLLLGDV
                     DSYKLQIGKIFDKYDIPYYFGKAEEMSHHPLVHFVESLERLRRYRFRAEDLLNLLKSG
                     LYASISQKELDLFESYILFADMKGQAAFSRAFSVNGRADYDAEVVKEKRLVYDLAVLE
                     PLRAKIMEPLNQLFKAGPQSGATLLEKFMAFLEAIEFPKNMEKMSRNLSEVEQEKEEQ
                     VWKSFTHLLENFHQIFGKEKLKMDDFLAILQAGMQASHYRTVPATVDVVNVKSYDLIE
                     PHTAKYVYAIGMGQSNFPKVAKNTSLLTEEEMEKVNLVSASSSRFDLVSRENIKKNHA
                     AMMSLLNAATEQLVISTPQIYNEGEDSLSPYVKILKEMGLSSEERGRIKSLSPQDIGH
                     YKSLLSRLIESERPSLDNEEWEGQRAFWTVLVRHLKKKLESQGIEIPTITGDITSKQL
                     AADTLAALYPEDKPLNLSASSLTNFYNNQYLYFVRNVLRLREQESIHPTAFQHGLFLH
                     RIFERVVMDQSELDFDQKVDKAILRTRDEAEFAMFYNQDADARYTEEVLDKIARSSAT
                     ILRDNDLVEIDGQEKSFRQDKALVFDLQNGKSVHVNGTIDRLDTLQINQAVGVVDYKS
                     SDQSFSVGDFYNGLKPQLVTYLAALQELDETKDKPVFGAMYLHLQDPIIKLKDTKNLE
                     QLEGAANTSLVYKGLFLKEESLGLNHFYQTRNQLYTEDEFAVLLNHNQELYKQAAMDI
                     LAGRFAINPYTKDGRSVAGEQLKAITGFEADRHMGMARRLVKEAKRQDWMERMKGGQD
                     "
     gene            complement(16173..>19938)
                     /locus_tag="ACHU66_000905"
     CDS             complement(16173..>19938)
                     /locus_tag="ACHU66_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFR3389201.1"
                     /translation="KQQFSIRKYHFGAASVLLGVSLVLGAGTQVVKAEEAVTSSEPTI
                     ASSVAPASTEAVAEEVDKTNAEATLVPAENTSAVATTSTEVEKAKAVLEQVTSESPLL
                     AGLGQKELAKTEDATLAKAIEDAQTKLAAAKALLADSEATVEQVEAQVAAVKAANEAL
                     GNELQKYTVDGLLTAALDTVAPDTTASTLKVGDGEGTLLDRTTTATPSMSEPNGAAIA
                     PHTLRDKVEATKESGWYTFESYDLNSYNRNQNGSIVGGTAVGAYIRYSLDSDPATSTV
                     LAELVSTKDGEVLESHKLEAGNSVTFSYPKTINAKNSNITLTYDTSTATADIPGSLKF
                     YDDRDAVYSTVVVPAYQVNTTRYVTEDGTVLATYSLQTIAGQTVTSSKVRTFTGYDYV
                     KTTQNAIQGAYPKGTLMLAGVGADKNGNKYYKAIREVVEDNQSVMTLYLLDPTYTGTV
                     DWTGTDTTGFIPLLKTSPTVYTIDRKVYDYNINATILSPYTVDNGFMVFKESATNAQG
                     SKYRVVAQWSGTDETDPTKPEYTGGKYGRIYIGTQVWTTAKELGITENQWRGTADKFD
                     NNGFVPFGTQQYLRNASVATAVETTHVYKVNPKFGDVIVEYYDTDGKQIVNSVVDTPK
                     SALGTEYNTDVDRRPASLVAADGTVYFYKEVKSDSAKTTGTVVAGTTTVKYVYEKAGS
                     VNVNFVDINGKVIKAPVSDEKDAKPGYNYDTDLDQKLASITFEGKEYKLVPAGDYPVG
                     KVGKGNNLIEVGNNTAKGIDPTTGKIEAGVNKEVTYVYKAVTGSVVVNYKDTEGNVIK
                     DPETDVSDAPVGDAYTTTDKKPNEIITKDGSRYVLVPSKTDGEENGKVIEGTITVTYV
                     YQKVANWIPEIPNVPETNRPKVPYPFDPTEPDEPIDPTTPGTNGEVPNIPYVPGYTPV
                     DPKDNTPLKPIDPNDPGKGYVPPTPENPGVDTPIPYVPVKKVVTNHVDEEGNPIAPQE
                     EGTKPNKSIPGYEFTGKTVTDEDGNTTHIYKKTPEVKNGTVVVNYVTEDGTVIKEPVT
                     DTPTSPEGTPYDTTDNKPKTITFKGEEYELVRVDGTENGKVVEGETVVTYVYRKVETP
                     AKKVVTNHVDEEGNPVAPQEEGTKPNKSIPGYEFTGKTVTDEDGNTTHIYKKTPAKKV
                     VTNHVDEEGNPIAPQEDGTTPKRQISGYEYVRTVVDEEGNTTHIYRKLSNKPTTPEKE
                     TPAKPQAGKTASGKAQLPNTGEASSVAGALGTAMLVATLAFARKRRRNED"
BASE COUNT         5294 a         4600 c         3988 g         6056 t
ORIGIN      
        1 gagaagagct tgcaagtgca agccttctga aattaatcta cttcgacaat ccagccttca
       61 ggagcttcaa tatcaccaaa ctggatacct gttaattctt catacaattt acgagtgaca
      121 ggtcctactt ctgtttcact atggagaaca tggagtttgt ctgcaatctg gataccacca
      181 attggtgaga tgactgcagc tgttccgcaa gcccctgctt cgacaaactg gtctaattct
      241 tcgacaagga cttcacgctc cacagccttc atacccagac gatgctctgc caaatacaag
      301 agagaatact tggtaatgga cggcaagatt gatggtgaaa tcggtgtgac aaactcgtta
      361 tcagctgtga taccaaagaa attagcagac cctacttctt caatcttggt gtgggtagct
      421 gggtccaggt agatgacatc tgagaagccc tgctttttgg catattggcc cggtaagagc
      481 gatgccgcgt agttaccacc aaccttagct gcccctgttc catttggtgc cgcacggtcg
      541 tacttgtcct gaatgaggaa gttggtcgga gccaaaccac ccttgaagta gttgccaact
      601 ggcatggcaa aaactgtgaa aatgtactct tctgctggtt ttacgccgat gatgtcgccg
      661 acgccaatca ggagcggacg gagataaagg gtgccacctg ttccataggg aggaacgtag
      721 tctgcgttgg ctttgaccac ctctttacaa gccttgatga aaaggtcagt cggtacaggc
      781 tccatgagca agcggtcagc tgtccgttgt aggcgctcag cgttttggtc tggacggaaa
      841 agttggagct ttccctcctt ggtgcgatag gctttaaggc cctcgaaggc ctgctggcca
      901 tagtgaaggg ctggggagct ttctgaaatg tgaagttctg catcttcggt cagctgcccc
      961 tcagaccact ggccgttttt gaaatgagcg atgaagcgat aaggtagttt catataagaa
     1021 aaaccaagtt tttcccagtc aatgttcact gtcatatagt ttctccaaat tcatttttta
     1081 ttattttact acttttgaaa gtgattttca attaaaaatt cagaaaattt tgtgaatata
     1141 agaaaaagcc tggtaaacca gactcttact ctttaaaaac aaattctagt tgaatttcag
     1201 gtgagatagc actaaataaa ttttgatagt aggttttagc tgactctttt atgtcatctt
     1261 gataatctgc taaaaacgtt tcttgttctg ctgactgaaa ttcatctgtg atgagctggg
     1321 cagtatcgat ttcttctgtc gcaaaactca gtatctcacc actcttatca tataaggtat
     1381 aaggttcttc tgcatctaat tccaatccaa tgactttaaa ggctggaagg gtaattttgt
     1441 accgattttc agaaagatgt tcaatggata caggactttt gatgccaaat tttgccttgt
     1501 acttaatgat taagagggct gatttttctg agaagggcat ttcttgacca aataagaccg
     1561 tgtagttttt agcggtcaca attttctgga cagctgtatt tagaaatacc aattcattga
     1621 cttgctcgac agattcaata gccgtatgga tactcacttc ttgctgatta ctcttttcgc
     1681 cagaaatgta gccgtatccc catacaccac acaataacag aagaaaggac agaacaagcc
     1741 atagatagac ttttatactg aaaaataatt tgataatttt catagcattt acctcctcta
     1801 cagtatagca aaaagactgc tttacaggcc tcgattagta ccaaccagtc agctggtgaa
     1861 gccagtcctc aaaggtagga aagactttca tggcaaggac tatcagtact aagatagtga
     1921 acaaaataag gaatttcttt ttcattctaa atacctaatc aatccaggct ctgaagactt
     1981 cttcatcgga aatggtatcc gaaatgaagc tgccgttgct agtgcgttca gacaggctaa
     2041 gctgggtcac atcaacctca tagagtttgc ctgttttgga ctgcacgtga aggatttgac
     2101 ttgtcgtttc atcttcattt gctgtgctaa agaggtcaaa atccccctgg tccgtcaaga
     2161 ctggacccgc cgcaaagaca cggtgaggct tggccttgag ttcgcgaagc acctgcaaac
     2221 cacgcttggc acggctggtc acagggattt cctccgtcgc catccgctta aagctaccac
     2281 gctgggtcag aagatagaca gagcttgtgt tgctgataaa ggccgcaacc accacatctc
     2341 catccttgag gttgaccgcc ttgacaccag ccgccttggc accgatgatt ggcacttctt
     2401 cgatgttaaa tcgcagagca tagccctttt ctgtcatgag catgatgtca tccaagacta
     2461 caggcgatac agtaatgacc acatcctctg catctttgag tttagcatac ttggttgact
     2521 tggacttgta agtccgccaa ggagtaaact ccttccgctc cacacgcttg atttgaccgt
     2581 attgggtcac cgcgaaataa gttccctctt caaaattctc caccaattca gcgaagataa
     2641 tttcttcact ggtatcaaag ttcatgagag tctggctcag gtgttcacca atatccttcc
     2701 aacgaatatc tgtcaactca tggacaggtc ggtagatgac atttccaaga ttggtaaaga
     2761 gcaataggtg ctgggttgtc ttggcattct gcaagaaaat cagctggtcg tcgtcccgct
     2821 tgcccatttc ttccaacgtt gaggcattga aagaacgcgg acttgtccgt ttgatataac
     2881 ctgccttggt cacgctgaca aaagtttctt cttcgacaat caagctggcg gtgtcaatct
     2941 caatcgtttc tgcctgatct tgtaactcac tcaaacgagg attgccaaat tgtttcttga
     3001 cctcacgaag ttctttcttc ataagattaa acatggtccg ctcatcgccg ataatggctg
     3061 ccaaggtctg aatctgctcg cgtagggctg cttcttcgtt ttccaaggta acaatgtcgg
     3121 tattggtcaa gcggtagagt tgcaaggtca cgattgcttc tgcctgttcc tcactaaaat
     3181 catagctgac tttcaagttt tccttagcgt cagccttgtt ttcagaagca cggataagag
     3241 caataacctc gtccaagatg gaaatgacac gaatcaaacc ttctacgata tggaggcgtt
     3301 tttctgcctt ttccttgtca aacttagacc gagcaatgat aatctcacga cggtgggcga
     3361 tatagctaga caaaatcttc tgcaagccaa cctgacgcgg tgtgaaatta tcaatagcca
     3421 ccatgttgaa gttgtaattg atttgcaagt cggtgtattt gtagaggtag ttgagaatgg
     3481 tctgctcatc gctgtctttt ttcagctcaa tggcaatccg aagaccagta cggtctgact
     3541 catcacgcac ctcggcaata ccaggcacct tgttgttgac acggacatca tcaatcttct
     3601 tgaccaaaac agccttgttg acctcgtaag gaatctcagt aatgacaatc tgtttcttgc
     3661 cagccttaag ttgttcaatt tcacaacggc tacgaacaac gacacggccc ttaccagttt
     3721 cataggcttt cttgatttcg tcagcccctt ggataatggc acctgttggg aagtctggtc
     3781 caggcaagaa ctccatcaac ttttctaact tagctgttgg atgatcaatc atatagacaa
     3841 cggcatcgat gacctccgct agattatgcg gcgggatgtc agtcgcgtac cctgctgaaa
     3901 ttcctgtcgc tccattaacc aagagattag ggaaggcagc tggtagcaca gtgggttctt
     3961 tttccgtatc gtcaaagttc caggcaaatg gcacagtctt tttctcgatg tcggccagca
     4021 gataaccagc catttcagac aggcgagcct cggtgtaacg catggctgct ggcggatcgc
     4081 cgtccatgga accgttgttt ccgtgcatct ccaccaaaat ctcgcggttc ttccagtcct
     4141 gactcatgcg gaccatggca tcataaatag aggaatcacc gtgcgggtgg aaattcccca
     4201 tgatatttcc gactgacttg gcggacttgc ggtaaccctt gtcaaaagtg ttgccgtcct
     4261 tattcatgga ataaagaatc cgacgttgca cgggcttgag accgtcacga atgtccggca
     4321 gggcccgctc ctgaataatg tatttggaat agcgaccaaa acgctctccc atgatgtcct
     4381 caagggacat gttttgaatg tttgacataa attaaaatac aaagggacga ttcagtcccg
     4441 tgttcagtta gacagactag ctgaacattc ctttctttgt ttcttggtgg aaagttaaat
     4501 tttctttctt ttttactttt ttgataatca ccttaagtag tgggaacgga agagacctta
     4561 tatagcaatt tctgttgcta tatccttaac taaacattag ttctttacct aagttctctg
     4621 ataaaagaaa gcaatcatat cccctactct atcataaaga ttatcatcaa catatgctaa
     4681 tttctcaatt tctaattcct catcatcaat caaattttct tgataagctt gagcatattc
     4741 accacacgat tttagataat ccattattaa aactaactct tgcttctgtt gagaagaaag
     4801 gacttttcct ttatagagag tctcctttat gaatcgacag tagtttgagt gctcttcaca
     4861 ccaattgaca ttgccatttg cttgagcttc ccagcgaagt ttttctaatt gtcgaagtaa
     4921 ttcaccttgt aaagtagaag aaatcccgta tttaggcaca tctgctcgcc aaattgaacg
     4981 tagaacctct gtatactgag aagcataagg aactttctta agttgctcga tagttagctt
     5041 ttttcttaaa aactttatca tagctactca tccactttcc actggcttgc ttgccagctt
     5101 ctaaagtcat cgacatctgt tttgattaat ttcaaacagg cattaaggac aaaggcatca
     5161 tcaagtagac ctgctccaag gatgaaatct ggtaaggcat caatagggct gaaaaaataa
     5221 atcaaagcac ctataattgc gaggattgtc cctttgggta acttgctgta atctctacgg
     5281 atatagcttc gcaccatgga aatcaagcct ggaatggccg caaacttatc tccaccaaaa
     5341 ggcatacgag acagtttccg ttccgtgcta tgtaaaaaat gttctaattt tccttgctca
     5401 gatagcaagt tttctgcatg ttgccgagtt ttggaagatt gccaaatctt caatgcttgg
     5461 tcaaatttct cttccaactc ctgtttgttt ttaaatggct tgcgctttcg agcccattca
     5521 gacaaccgtt tttgaaaacg gtcagttaat tttggcataa aacacctcac atagtttgct
     5581 tgtgctagag ttacaaatcc tctgtgcgat agagattgta tccttcatag taataagaat
     5641 ggtcgatgcc tttgaaataa gtagcctgac caagatcttc cgttaaggct tgcaataaca
     5701 gatatttaag ctctcctgtt gacacatggc ttctaaccat tgcattgaaa tattcatctt
     5761 tatcaatgct attccagtca acaaccttac ccaaactatc ccgtagcata caatccaacc
     5821 agatacgcat actgcgtcca tttccttctc ggaatggatg tgcaatgttc atatctgcat
     5881 atttttcaac gatttcgtca aaggtttcat gaggtagctt gtcgatatag gccaaagact
     5941 gctccagaaa gattcgtgga gcaaattgaa aattatcctt ggcaatatta acgtcgcgaa
     6001 tcttacctga aaagtcgtaa atctcttcaa acaaggtctg atgaatgtgt gccaaacctg
     6061 caaaggttcc aacttctatc tggaataact gaccagtttc gaacagttga gctgcttttt
     6121 tcttgctgat ttgttcttca agacgagcta attcagccga attttcaatc cccaatttat
     6181 tttctaaaac catatggcac ctcaacttcc ctgagattat ttagtaaaag ctgtcgcttc
     6241 ctccaaggta aacttcacat tgtcctcaat ccacttgcgg cgtggctcga ccttgtcgcc
     6301 catgaggaca gatacacggc gttcagcacg ggccaggtct tcaatggtga cacggatgag
     6361 ggtacgggtt tcagggttca tggttgtttc ccagagttga tcggcattca tctcaccaag
     6421 acccttgtag cgttggagga taaagccctt gccaaaatcc ttgcggagat cttctaattc
     6481 tccgtcagac caagcataag caatcttttc tgtcttgccc ttgccttttg acatcttata
     6541 aagtggcgga agggcgatat aaacccgtcc tgcttctact aacggtcgca tatagcgata
     6601 gaagaaagtc agcaagagag tctgaatgtg ggcaccatcc gtatccgcat cggtcatgat
     6661 tataatcttg tcatagttga catcttccag cgtaaagtct gaaccaacac ctgcaccaat
     6721 ggtgtaaatc atggtattga tttcttcgtt tttgaggatg tccgccattt ttgccttggc
     6781 ggtattgata accttaccac gcaaaggtag aatggcttga aacttgcggt cacggccttg
     6841 tttggcagaa cctccggccg aatctccctc gaccagatag agttcatttt tggctggatt
     6901 tttggactgg gctggagtga gtttccctga caaaaggccc ttatccttct tgtttttctt
     6961 accgtttcgg gattcgtcac gggccttacg agcggcctcg cgggcgtcac gcgccttaat
     7021 ggctttacga actagatttg aagctaattc cccattttcc aaaaggaaga atgttaactt
     7081 gtccgataca atgccgtcca ccaccggacg agcaagtggg cttcccaact tgtccttggt
     7141 ctgcccttca aactgcaaat gctcttctgg cacaaggata gaaaggaccg cagacaaacc
     7201 ttcacggtag tcagagcctt ccaagttctt gtccttttcc ttgagcaagt tggtcttgcg
     7261 ggcgtagtcg ttcatagcct tggtaatggc tagtttgaga cctgtttcgt gggtaccgcc
     7321 gtccttggtc cgaacgttgt taacaaagga caggatgttg tctgaataac cgtcgttgta
     7381 ctgcatggcc acttgaactt ggaatcctgc atcttctcct tcaaaataga ggactggtgt
     7441 cagagtttcc ttgtcttcgt tgaggtagga aacaaagtcc tgaacgccat tttcgtagtg
     7501 gtactcctcc tgctcacctg ttcgctcatc tgtcagagtc atggtgactt gtttgagcaa
     7561 gaaggctgat tctttcaggc gttcggcaat ggtattgaac ttgaagtccg tcgttgaaaa
     7621 gatggtatcg tcaggcataa aggtaacttt tgtacctgtt tttgattttg gagcggtgcc
     7681 aatcttctct agggtcgtga ctggctttcc gccctgctca aaccgttgct tgtaaacagc
     7741 tccgtcacgg gtaatctcaa cttccaacca gctagacagg gcattgacca ccgaagaacc
     7801 aaccccgtgg agaccacctg aagtcttgta gccaccttga ccgaacttac ctcctgcgtg
     7861 gagaacggtg aagataactt cgactgttgg tttgcctgtt gcgtgcatac caacaggcat
     7921 cccacgtcca cggtcggaaa cagacaggct accatccttg ttaatagtca cgtcaatccg
     7981 atcaccgaaa ccagacaagg cttcatcgac agcgttatcg acaatctccc agaccatgtg
     8041 gtgaagacca ttcccgtctg ttgagccaat gtacattcca gggcgtttgc gaacggcatc
     8101 taggccttcc aatacctgaa tggcatcatc attgtaatta tttatatcaa tctctttctt
     8161 agccaaaatt gacctccata cttatcatcc tttctatatt acaagttttt tagcattttt
     8221 gcaaaatatt tctgttacct atcggctgag acaactcaca aaataagatt ttggactaaa
     8281 ttagatgaaa atatgctata atgaaaacat gttagtcaat cttattttac tatttattgc
     8341 atatttgttg ggatctatcc cgtcaggact ttggataggt caaacttttt tcaatatcaa
     8401 cattcgtgaa catggttcgg gaaatactgg aacaaccaac acattccgta ttttaggtcc
     8461 aaaagctggt acgattgtat ttgtaattga ttttctcaag ggaacgttgg cagccttgct
     8521 tccgctgatc tttcatgccc aaggaatttc tccgattgta tttgggttaa tggctgtact
     8581 ggggcacact ttccctatct ttgcccaatt caaaggtgga aaggcggttg caacatctgc
     8641 tggtatgctt ccgggcgtcg cacctgcttt ctgtctctat ctagtcatca tttttgtgac
     8701 ttctctttac ctcacttcga tggtttcctt ctctagcgtc ttagcagcag ctttagcaat
     8761 cttaggagct ctgctatttc cagcaatagg aatcatcctg ccaagctacg actggctctt
     8821 tacagtgatt atcgtctttc ttggcagttt tgtcatcatc cgtcacaagg aaaatatcca
     8881 gcggattttg aaaaaagaag agaaccttgt cccattcgga ctcaatctaa ccaaacagaa
     8941 aaagcgagct tagtactcgc ttttagttta ccttgacttc gaccacttct attctctccc
     9001 caccaagcaa aatcaaatgg cagtctacac gatctatctt gtaggctttt ttcaatgcct
     9061 cggcatagag ttgcatttga gcttggtaac gttggctgag ttggcttggt tggtcatatt
     9121 tgtctgtctt gtaatcaaag aggacaatat gatcatcata tagtaggtag ccatctataa
     9181 tcccacgaag gacaaagttt tcctgagata cagaatctgt ctgcaaactt gcaaatggtg
     9241 cttctcggtg gagtttgtcc gtattggcta gaatttctct tcctaagtct gtatcaaaga
     9301 aatccaaaat catctgaaca ttgattttat ctttaatcgc ttgctcagca tgaacagctt
     9361 ctagggcttc tcttaccgta tcttctgtca ccaaccaaga cagatccaag cgttgcatga
     9421 gttcatgcac tgctgaaccg acttgggctc cagtgatttt aggtttcttg gagaaatctg
     9481 gcaagttgaa ggtccgtttt ggctggtatt tttccataac ttccatgcct tcttgttcca
     9541 aaataggctc atagagtttc ttgatttgac ttggagtccg taaacttggc aattcaatgg
     9601 caaccttata gcgttcgttc aactcttgaa cagaagacaa ctggtccaag gcctgagcaa
     9661 tgtcttctga ttgacgaata tctttaaggc tggcatcttc tagcttactc ttcaatgtaa
     9721 gttttccaat cttctcctct gtcaaatcct catccgtcac aaagactttc ttgaagtgca
     9781 agtcttctcc tgagaaggcc tcatcaatgg ctagaatcca gtcttggaag gtcgccacgc
     9841 tttcacgagt tgattgggct agtacgccat ctgccttttt gccatcgtat tttttagcta
     9901 acttatcagc attgcccttt ccaaccagat acagcttctt ctcggcacgc gttagtgcaa
     9961 cataaagcaa ccgcatttgt tctgaaagat tggcaatttt tagctcttgg agattatctt
    10021 gataaggaag ggtgttcatt aagactcgca cctgaggtaa tgggctattc accttatctt
    10081 tcatatccgc taaatattgg atacctagac catttttccg gctgatgatg actgactggt
    10141 aatggtcttc caagttgaat cgtttatcga tgttcatgag gaagacatat ttgaactcta
    10201 gaccttttga cttatgaatg gtcatcagct gaacagcatt ttgcggtaga aactcttgta
    10261 catctgccaa gtccttgtca tttgctaagg cacggtcaat catgacgatg aaacgagaca
    10321 agcccttgta gcctgttttt tcaaactggt tggcacgcaa acctagggca tatagatttg
    10381 cctgacgctt gctgccattt ggaagagcac cgacatagtc atagtagaat ttttcattga
    10441 aaatcttcca aatcaggtca taaatagaat gcattttggc aaaagcacgc cagttttcca
    10501 aaatagataa gaaatccttg attttcttct tcaactctcc tgacataagt tcaggatgtt
    10561 gcccatttgt ttgctgggct atttccattt tctggtaaaa gaaacctgtc ccagcctgca
    10621 aggaaatccg tgtcagctca tcctcatcga atcggaacat aggcgatttg agaagagcaa
    10681 ccaaggcata gtcattgagt ggattattga taacccgcaa ggtatccagc ataatcatga
    10741 cttctaaaga ttgcagatag gaggctgctc cgccatctgc cacaattgga atcccatgtt
    10801 tttcaaaaat ggatataatg aggtcattgt gcgtccgttt ttgcaccaag agagtgatgt
    10861 ccttgaagtc tgctccttct tcattgtgaa gacgaataat ctcttttgca acaacctcga
    10921 tttcaccagc agtcagaggt gtttcttcct cagcatccgt tgaagagttt gcgctatcct
    10981 gctggtcgta aatcaagtat tccatctcat gcttgggctg ggcaattttt tgaccaggac
    11041 taccagccac tagactgtgg gtttcatcat acttgatttc ccctacttga cggtccatca
    11101 ggcgcgtgaa aatagcattt gtcgcctcca agacctcaat ctgactacgg aaattttcct
    11161 taagcaaaat caattttcca gcctgagaat ctgcctggta aagttcaaat ttctcttgga
    11221 aaatcatcgg atccgcctga cggaatcgat agatggactg cttaatatcg cccaccataa
    11281 agcggttgtg accgttagac agcaggtcca gcattcgctc ttggctatgg ttgttatcct
    11341 ggtactcgtc caccatgact tcgtggtatt tttcttggaa aaactggcga atgtccacat
    11401 tttcttctaa aatacgaata gcgaaatgcc caatatcgcc aaattcaaaa gctgcttcct
    11461 tgattttgac atccagataa gcctgcgaaa aatccaagac aaagtcacgt aataatatca
    11521 gcattggcaa agcttgaatc tgatagtctt tcagcagggt taattcataa acttcctgcc
    11581 ccaaatctcg tagtccagag ataatctggc tcttgccagc attgtattct tctttaaagg
    11641 ctatcaaatg ctcatctttt ggtcgattgg cattggtcag acccttgcct ctggactggt
    11701 cgttgataag aaggactttt tcaacccgct ccatcaagtc cttctggtcc agactatcta
    11761 aacctgtcag caaatctaaa acttcttcca catttgcaaa atacttggcg gaaccaaagt
    11821 catttctgcc ccattccaca tggtagcgga agaaatctgc agcttgatag agcttgtcta
    11881 acactttttc cttaaagcct tgctctaaga tcagctctat acgctcttga ctgtacaaat
    11941 cagcttgaac agcctgctct ttcagccatt tagttggact gcttgtcgat tggctaaaat
    12001 catgcacctg ataaacaacc tgacggaaac caccattatc cttccggttg cctgaaaaat
    12061 tgcgtaccaa ttttcggaat gcaccatttt catcctgttc aagataatct gcaaagagct
    12121 ggtcaaaaac ctcttttttc agactcgctt tttctgtctc atcctgtaaa atccgaaact
    12181 gtggagaaat gccaaggcta taaccgtaag tcgttaccaa tttctgcgta aaggagtcca
    12241 ttgtcccaat atccgccttg gtcaaatccg ccaactgggc tgacaaatgc cgacgcaagt
    12301 ccatatccat cgtctccgca atggtttcat tgagattttt ctcgatccgc tccttcaact
    12361 caccagccgc tttgactgta aaggtcgaga taaagagctg atcaatccct acgccacgct
    12421 tcaacttatc caaaatccgt tgaaccatga cgaaggtctt tcctgaccca gccgatgcag
    12481 agaccagaac attctgaccg tgcgtgtaga tggcttcaat ctgctcaggt gtgcgttttt
    12541 gttgcttatc agaattggct tccgccagct gcacagcctt gatttcttct gcgcttaaaa
    12601 attgttcaaa agccattagt cctgacctcc tttcattcgt tccatccaat cttgacgctt
    12661 ggcctccttg accaaccgcc gtgccatgcc catgtgccga tccgcctcaa aaccagtgat
    12721 tgccttcagc tgctcacctg ccaccgaacg cccatctttg gtataagggt taatagcaaa
    12781 gcgaccagcc agaatgtcca tggcagcttg tttgtagagt tcttggttgt ggttcaataa
    12841 aaccgcaaac tcatcttccg tgtagagttg gttacgagtc tgataaaaat gattgagccc
    12901 cagactttcc tccttcaaga aaagcccctt ataaaccaag ctagtattgg cagctccttc
    12961 tagctgttct aaattcttcg tgtcttttaa tttgataatc ggatcctgca aatgcaaata
    13021 catagctcca aaaacaggtt tatccttggt ttcatccagc tcttgtaagg ctgccaagta
    13081 ggttaccaac tgaggtttta gaccattgta aaaatcccca actgaaaaag attggtcgct
    13141 ggacttgtaa tccacaacac caacagcctg attgatttgt aaggtatcca agcggtcaat
    13201 ggtaccattt acatgcacac tcttgccatt ttgaaggtca aaaactagcg ccttatcctg
    13261 acggaaactc ttttcctgac catcaatttc aaccaaatca ttatcccgca aaatagtcgc
    13321 actggaacga gcaatcttat ccagcacctc ctccgtataa cgagcatccg catcttggtt
    13381 ataaaacata gcaaattctg cctcgtcacg cgtacgcagg atggccttat ccactttttg
    13441 atcaaaatct agctcagatt ggtccatgac cactcgttca aaaatccgat gcaagaagag
    13501 cccgtgttga aaggccgtcg gatggatgga ttcctgttcc cgcaagcgaa gaacattacg
    13561 gacaaagtaa agatattggt tattgtagaa attggtcaag ctggatgcag aaagattgag
    13621 cggcttatcc tctggatata aagcagccaa ggtatcagct gcaagctgtt tagaagtaat
    13681 atcaccagta atggtcggaa tttcaatgcc ttgcgactcc aatttcttct tcaaatggcg
    13741 aaccaagact gtccagaagg ccctctgccc ttcccattct tcattatcaa gagatggtct
    13801 ttctgactca atcaaacggg ataagagact cttgtagtga ccgatgtctt gcggactaag
    13861 acttttaatc cgaccacgtt cttctgacga aagccccatt tcttttagaa tttttacata
    13921 aggagaaaga ctatcttcgc cttcattata aatctgagga gtggaaataa ctaactgttc
    13981 cgtcgcagca tttagcagag acatcatggc agcatggttc ttcttaatat tctctcggct
    14041 gaccaaatcg aaacgtgaag aacttgccga aacaagattg actttttcca tctcttcttc
    14101 cgtcaacaga ctggtattct tggcaacttt tggaaaattg gactggccca ttccgatagc
    14161 ataaacatac tttgcagtgt gtggctcaat caaatcatag gatttgacat tgaccacatc
    14221 aaccgttgca ggaacggtgc gatagtgact ggcctgcatg ccagcctgta aaatagccag
    14281 aaaatcatcc attttcaatt tttcttttcc gaaaatctga tgaaaattct caagaagatg
    14341 ggtaaaactt ttccagacct gctcttcttt ttcttgctct acttcactca aatttcgaga
    14401 cattttttcc atatttttag gaaactcaat tgcctccaaa aatgccataa atttctcaag
    14461 caaggtagct ccagattgtg gtccagcctt aaacagctga ttcaaaggct ccataatctt
    14521 agcacgtaga ggttctaaga cagccaaatc atagacaagc cttttctcct taacgacttc
    14581 agcgtcatag tccgctctgc cattgaccga aaaagccctc gaaaaagcag cctgcccctt
    14641 catatccgca aagaggatgt aggactcaaa caagtccagc tccttttggg aaatgctggc
    14701 gtacaaacca gatttgagga gattgagcag gtcttccgca cggaaacgat agcgacgcaa
    14761 gcgctccaag gactccacaa agtggactag gggatgatgg ctcatctcct ctgccttacc
    14821 aaaatagtag ggaatatcat atttgtcaaa aattttgcca atctgcaact tgtagctatc
    14881 cacatcaccc aagagcaaca agatgtcttt atagcgagct ccttggtgta tgtgttggcg
    14941 aattgccgtt gctacctgct ccacctcttc tttttggtta acgacttccc atagctggat
    15001 tttttcttga tctgctggag tcaagtctat catggtcccg ctgtagtcat aataggcttc
    15061 catattcttt gaaaatttac caatactgtc caagactggc tcttgaccaa tataagtagc
    15121 cttagtctgg aactgcgctg atagctgacg taaaaagtca acgttggctt gatagacatt
    15181 gccttccgca tagatgactt gcacagcttt cttgctggca tatacaccaa ttaggatttc
    15241 cgacacccgt tcatttaaag cacttactaa agcttcttct tctgccgaaa aacgagaaaa
    15301 tccgtctacc accagcacaa tgttcttcaa ctgctcatcc aactgaccag tttctaccaa
    15361 gccagtcaac tgtgctaact tagactgatg ctcaaagctt tctttggaca aaatgtctgt
    15421 tacagccaga aaaatcttta ccaaatcagc ctgcttatct ggtgagtcca tggcttccaa
    15481 gtccaaaata gacatatttg cccgttgcaa ttctttgtat aaagcaacta gctggttaat
    15541 gaaaccaaaa tctctctgca aacgaccata gactttcaag tcgccatctt caaattgcga
    15601 tagaacacga aagaaaatca ttgccagacc aacatcatcc agcggttgac ctgcttcttt
    15661 cctatcaatc atcaaatagc gggccaattg cccaaaacgg gtcacgataa tatcaaaagt
    15721 tgcctgttct ggcaggtatt ccaagacctt gcgttccatc tcaaaggata gagagtttgg
    15781 agcaatataa aaaactcgct tagcttcctt agcaaattct gccgaggtat ccgttaaata
    15841 ctgcgtcaga ggattgcgaa tatctgtata gactaatttc atatcaaatt ctcatgcttt
    15901 cgttgaatat actatatcta gtatatcaaa ataagacaaa aaaatgggag cgagaaagaa
    15961 ctcgaccgaa taaaaaaaga gctcgtctcc cacccccaca cagctccaac agtcagagta
    16021 gtgactgttg gaggttggaa atggagcgaa ctctaagatg taactctgga ctttttgcac
    16081 agcctcattt tttcggaaag gcaatcctgc atgttacgta caattcttat actttctata
    16141 tgggggaata aagtctgtat aaagaatttt gactaatctt cgttacgacg acgttttctt
    16201 gcaaacgcaa gtgttgcgac aagcattgct gtaccaagcg cacctgccac agatgaagcc
    16261 tcaccagtat ttggcaattg agctttacct gaagcggttt tacctgcttg aggttttgca
    16321 ggagtttcct tctcaggtgt tgttggttta ttagaaagtt tgcgataaat atgtgtcgtg
    16381 ttaccttctt catcaactac agtacgcaca tactcgtaac ctgaaatttg acgttttggt
    16441 gttgtcccat cctcttgtgg agcaataggg ttaccttctt catcaacgtg gttagtcaca
    16501 actttcttag caggtgtttt cttgtagatg tgagttgtgt tgccatcttc gtcagtaaca
    16561 gttttacctg taaattcgta acctgggatt gatttgtttg gttttgttcc ctcttcttgc
    16621 ggcgcaacag ggttaccctc ttcatcaacg tggttagtta caactttctt agcaggtgtt
    16681 tcgactttac ggtaaacgta agtcacaact gtttcacctt ctacaacttt accattttct
    16741 gtaccgtcaa cacgaaccaa ttcatactct tcacctttga aagtgattgt cttaggtttg
    16801 ttgtctgtag tgtcgtatgg tgtgccttct ggagaagttg gtgtatctgt tacaggttcc
    16861 ttgataactg tgccatcttc tgttacatag ttaacaacaa ctgtaccatt cttaacttct
    16921 ggtgttttct tgtagatgtg agttgtgttg ccatcttcgt cagtaacagt tttacctgtg
    16981 aactcgtaac ctgggattga tttgtttggt tttgttccct cttcttgcgg tgcaatagga
    17041 ttaccttctt catcaacgtg gttagttacg acttttttaa ctggaacata aggaattggt
    17101 gtatcaacac ctggattttc tggtgttggt ggtacataac ccttacctgg atcatttgga
    17161 tcaattggtt tcaacggcgt gttatcctta ggatcaaccg gtgtatatcc tggaacgtaa
    17221 ggaatatttg gaacctcgcc atttgttcct ggtgtcgttg gatcgattgg ctcgtctggc
    17281 tctgttgggt caaatgggta aggtactttt ggacggtttg tttctggtac atttggaatc
    17341 tctggaatcc agtttgcaac tttctgataa acataagtta ctgtgattgt tccttcgata
    17401 actttaccat tttcctcacc atctgtctta gatggaacaa ggacatagcg tgatccatct
    17461 tttgtgatga tttcgtttgg tttcttgtca gttgtagtat aagcatctcc aaccggtgca
    17521 tcagacacat ccgtttctgg atctttaatc acattacctt ctgtatcttt gtaatttaca
    17581 actacagaac ctgtcactgc tttatagaca taggtaactt ctttgttaac accagcctca
    17641 atcttgcctg ttgttgggtc aatacctttc gcagtattat taccaacttc aatcaagtta
    17701 tttcccttgc caactttacc aaccggataa tcaccagcag gaacaagttt gtattccttg
    17761 ccttcaaaag tgatggaagc taatttctga tccaagtcgg tatcataatt gtaaccaggt
    17821 ttcgcatctt tttcatctga aacaggagct ttgattactt taccattgat gtcaacgaag
    17881 ttaacattaa cgctaccagc tttttcgtaa acatacttaa cagttgtcgt acctgcaact
    17941 actgtaccgg ttgtcttagc tgaatcagac ttaacttctt tgtagaagta gactgtccca
    18001 tcagcagcaa ccaagctggc tggtctacgg tccacatctg tattatactc tgtgccaaga
    18061 gctgacttag gagtatctac aactgaattt acaatttgtt ttccgtcagt atcgtagtac
    18121 tcaacaatga catcaccgaa tttaggattt actttgtaga catgggttgt ctctacagct
    18181 gtagccactg aagcgtttcg gagatattgt tgggtaccaa atggaacaaa accattatta
    18241 tcaaacttat ccgctgtacc tctccattgg ttttcggtaa ttcccaattc ttttgcagtt
    18301 gtccaaacct gagtaccaat atagatacgt ccatactttc ctccggtata ctctggttta
    18361 gttgggtcag tttcatctgt gcctgaccac tgtgcaacga cacgatattt actaccttga
    18421 gcattggtag cactttcctt gaagaccatg aaaccattat caactgtgta aggagataaa
    18481 atagttgcgt tgatattgta atcatatact tttcgatcaa tagtataaac tgttggagat
    18541 gtctttagca gcggtatgaa tcccgtcgta tcagtcccag tccaatctac ggtacccgta
    18601 taagttggat ccaataagta caatgtcata acggattgat tatcttcaac aacctcacga
    18661 atagctttat aatatttatt accatttttg tcagcaccga caccagctag cataagtgtg
    18721 cctttaggat aagcaccttg aatagcattt tgcgttgttt tcacatagtc ataaccagta
    18781 aaagtacgta ctttagatga agtaaccgtc tgacctgcaa tagtttgaag actgtaagtc
    18841 gccaaaactg tgccatcttc agtaacatag cgagtcgtat taacttgata tgcaggcact
    18901 acaactgtag aatatactgc atctctatca tcataaaatt tcaacgaacc tggaatatca
    18961 gcggtagctg ttgaagtatc ataagtcaaa gtaatgttgc tattcttcgc attaatcgtc
    19021 ttcggataac taaaagtcac actattccca gcctcaagtt tatggctttc taagacttct
    19081 ccatctttag tactcactag ctccgctagt acagttgatg tagctggatc cgagtccagg
    19141 gaataacgta tgtaagcgcc gacagcagtt ccaccaacaa ttgaaccatt ctggttccga
    19201 ttgtaggaat ttaaatcata tgattcaaaa gtataccaac cagattcttt tgtagcctca
    19261 actttatctc gaagtgtatg aggagcaatt gctgcaccat ttggttcaga cattgaaggt
    19321 gttgctgttg tcgttctatc tagaagggta ccttcgccat caccaacttt caatgttgat
    19381 gcagttgtat caggtgctac tgtatcaagt gctgctgtta agagaccatc tacagtgtat
    19441 ttttgcaatt cattccctag cgcctcgttg gctgctttaa ccgctgcgac ttgcgcttca
    19501 acttgctcaa cagttgcttc tgagtcagcc aaaagtgcct tagctgctgc aagttttgtt
    19561 tgagcatcct ctatagcttt tgcaagagtt gcatcttcag ttttagccaa ctctttttga
    19621 ccaagaccag ccaaaagtgg tgattctgat gttacctgtt caagaacagc tttcgctttt
    19681 tcaacttctg ttgaagtcgt agctactgca ctcgtatttt cagctggtac tagcgtagct
    19741 tcagcatttg ttttatctac ttcttctgca acagcttctg ttgaagcagg cgctacacta
    19801 ctggcaatag ttggttctga tgaagtaaca gcttcctcag ctttaacaac ctgtgtaccg
    19861 gcacctaaaa ctaacgacac accaagcaaa acacttgctg caccaaaatg atacttacga
    19921 atcgaaaatt gttgtttc
//
LOCUS       DAZQCJ010000027        19813 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000027 DAZQCJ010000000
VERSION     DAZQCJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19813
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.027"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            47..355
                     /locus_tag="ACHU66_000906"
     CDS             47..355
                     /locus_tag="ACHU66_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR3389202.1"
                     /translation="MEHEMININAHLVKDVIFSSFETEDGIVPVVNFSLVKKYGKGKE
                     YTNCSAYGDKVEIAKEFEKGDLIHVFGYFKENRKGDKVYKNFIVKSMNKIEIEQMEEE
                     "
     gene            355..570
                     /locus_tag="ACHU66_000907"
     CDS             355..570
                     /locus_tag="ACHU66_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFR3389203.1"
                     /translation="MDFFTQGVDVLKTLVIAIGGGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGLKLIPLLANLIQ"
     gene            579..1442
                     /locus_tag="ACHU66_000908"
     CDS             579..1442
                     /locus_tag="ACHU66_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFR3389204.1"
                     /translation="MFDIFGKLEEMIQEILLDMIKGTLTSMFTDLNHQLDFVSGQVGQ
                     SPSQFNGEVFSFIKHINDSVVLPIAGLVITAVLCLELIQVVMQKNNMAEVDTFEFFKY
                     VIKMWVAVYLVSHAFEFAMAAFDVGQHMIGQATGVIHSETSISSEQITAMVDSLKEKN
                     IGELVGIVFELQVVKLTIQIISLIILIITYGRMFEIYAYSSVAALPFATLGNRDWGQL
                     GHNYIRGLFALALQGLFLVICFGIYAVLLRGVKMTDIHMSVFSILGYTVLLGFMMLKT
                     GSLARSIMNAH"
     gene            1455..1823
                     /locus_tag="ACHU66_000909"
     CDS             1455..1823
                     /locus_tag="ACHU66_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFR3389205.1"
                     /translation="MAYVQIPKDLSKVKTKIAFNLTKRQLIGFSVAGLASLPVYLSIR
                     ESVGNDLAMLVFIVLAFPFLFLTFYEKDGLTSEIYLKYIFLQQHYQPCVRYKRTLYKK
                     MHKEEVHETRKKKKTGTRVS"
     gene            1780..4209
                     /locus_tag="ACHU66_000910"
     CDS             1780..4209
                     /locus_tag="ACHU66_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFR3389206.1"
                     /translation="MRPERKRKQAQEFLEAKEVQLKKIQKETLKQDNNTLLGSIKSFF
                     ELGDKCLTAEDTIPIEKVYENGIFQLTDGYYTKMIAFEDINYQLALEEQRDFIFNQFA
                     AFLNSFDNSTRLQLCFTNQLGRLDELADAITIPSKDDGLEAIRCEFRVMLQNQLEKGN
                     NGLRQTKYIVFGVKEDTYRQAKVVLSRLEIDLQSHLKGMGIRSIVLNGFERLIVLHHI
                     LNPTEQVSELPEDLGNSDCRSSIVPDKFHFQKKYFSMDNVLCQTNHVQILASELSDRF
                     LTELLSLEENMVLSLHIQALDQVEAIKLVKRKFTDVQRMTMEEQKKAYRSGYDLDIIP
                     SDLMTYGTDLKHLLNDLQSRDEKMFLVSFILLNQGLTKAKLDNAVSQLATICSRHNCM
                     LKCLDYQQEQGLISALPLGMNCIKIKRQLTTSSTAVFIPFTTEELFMTSSTSIYYGLN
                     AISHNLIMADRKKLKNPNGLILGTPGSGKSFSAKREISNAILVTDDDILICDPEGEYG
                     ALVEAFGGEVIHVSAKSKDFLNPLDINFNYGDGDAPLKDKANFIMSMLELVVGGSGLT
                     AAEKSVIDRCLPKIYEAYFENPIPENMPILQDLYDLLLKQEESVGRKLATEMEIYVTG
                     SLNVFNHRSNVDLNKSLLCFDIKELGTQLKKIGMLVIQDQVWNKVSQNRQSQKSTRYY
                     IDEFHLLLKEEQTAQYSVEIWKRFRKWGGIPTGLTQNVKDLLASKEIENVFDNTDFIM
                     MLNQASGDREILARKLKISPYQLNYVTNSNAGEGLLFFGNTIVPFLDQFPKNTLLYQK
                     MTTKPEEVRKG"
     gene            4217..7438
                     /locus_tag="ACHU66_000911"
     CDS             4217..7438
                     /locus_tag="ACHU66_000911"
                     /inference="COORDINATES: protein motif:HMM:NF045974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFR3389207.1"
                     /translation="MGKSKRMVRETNKKHYTKSKPDISQRKEEQDFSGRTVDESVKFQ
                     HQIIHKESKLESRKNQVESSGKRRKNINIVDDKSTQTPLPIHDKNESTIQITDLQDEI
                     VNKNYLHQPSPPEAPLVESKPISHKKQVQLQQRKAFENQQNVESRNDIEFTEQNGHNS
                     EIHVSSDGDLAQTGNEQEVQQIQFEIVHHNPVNSSELNHSKELNFGPTSSQTQAQNNS
                     QPTESIDRKIHDTPFRKFDTNQSRIIDTETKFTHRRNEGRAKLIHNQNQGTPSQFKNK
                     KGKKHVHGDSVHESQNILSELPISQFDSSTDSVPIKTYPRVSDESNSIIDQADNESVF
                     DVSLLQSQLRKDNKKFQPSNSKLSKKSGVKIIEAKNSQSQQIEKTVTLTDGGKQSSNQ
                     FQSNLNENSIMIEQISYPNIDWSKKLHLPQIKLFSRDILHSNAVSGKIQNDESKPLTR
                     KGKRLSNRLSNKATKSNIARSLYPDTTSDKNRKTVSKSSKTKLSITPIQVDGRPKYYF
                     ETTTKKQFYKGRPSSTNNIKANKFRRQRLLKDQLQIHEKLNSLVIEPIEFVKDKKNRR
                     LLKKIEQAKLGRIKSSPLKKVVSGLASSEFLAASYLQAGADDNVAVDATSKVLNINAA
                     ILMRLAKKSNRNQTIKKLTKKAKVRQSQLEFRHKYRLMKEDKIFQRQSFYRKSMYRQR
                     MKQQIRNKHFPRIRDRIKEELKKLLNQMVQFITTKWKSILVLALALFSLLTVVYSNSQ
                     FMFGALSSVSYQLTTTSYLSSEEMLVALNHIFSTYELNLSNQLARLKESKPGYDEYIV
                     KGREQIGHDPHVLLAYLTARFGEIKDQTSVEPAMKQLFVQIYQVSYREEREIRYRKVK
                     EEVLDTSGKKTIVEKEVPYTYRKLIATVSKNDMDVVIQSIFAGSPSNLEHYRILRETK
                     GNLETAFPSGTTISLPGNVSSVYDVNLTGGNFPPPNPNHVAALNGGYPGQCTWYVYNR
                     FSQLGKPIQHSPMGNGGEWAFYAAKYGYPVSREARAGTAVCMPPTVPYADPTYGHIAF
                     VERVNPDGSIVISEMNVKGEFVISTGYLPREMAAQCYYINFGL"
     gene            7449..7664
                     /locus_tag="ACHU66_000912"
     CDS             7449..7664
                     /locus_tag="ACHU66_000912"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389208.1"
                     /translation="MRKDIQMLQKKKEKLLEKKQKIDQDLAQVLQEEQDLIYEEVIVV
                     FEKSNLSLEEFTKKVLFEKELSHETII"
     gene            7648..8244
                     /locus_tag="ACHU66_000913"
     CDS             7648..8244
                     /locus_tag="ACHU66_000913"
                     /inference="COORDINATES: protein motif:HMM:NF025641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFR3389209.1"
                     /translation="MKRLFSFVRVASLVMLFSGCQTIYASSPSSEPNSQPVAISVHPQ
                     ASEQSNAKGQVTENVDANNQDYEITDSVATKRQFVTFTSKSGKVFHLIIDHDKGGQNV
                     QLLTEVSEQDLLNLVESTDTVAVKPQKVEEVVDDKPVKNEEPKQKSSAGSYIIIGLFL
                     LGVLCVGYYIKVIKPKKEHHFEEFEEDDDYISDGEEEV"
     gene            8287..8925
                     /locus_tag="ACHU66_000914"
     CDS             8287..8925
                     /locus_tag="ACHU66_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HFR3389210.1"
                     /translation="MDLSSKQIKNLSHNKWYHATLKRHLSSLLEGIKVDYNLGHELDF
                     GAGFYITSDFQQAQKYINRLVEFLNTNKAGAEFLGNMDEEGIILEFELTDFPSIFTTS
                     NYKCHYFKSHNISDDVIDFAEFVFQNRLRSKERIHDFDFIYGVQTDDNPTGLLQKYKA
                     GLVSKADVLNEFRKPFSFKQLSIHNQEFCDIMKVTKIYLSKDGKELDQWQKQ"
     gene            8910..9140
                     /locus_tag="ACHU66_000915"
     CDS             8910..9140
                     /locus_tag="ACHU66_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389211.1"
                     /translation="MAKTMISPVELYSNELAQALLETSKYRLEASVAHQIARQYASQV
                     DFEDPILMHVGVNSIASTLIDKIKPEYFQTTS"
     gene            9217..10401
                     /gene="dcm"
                     /locus_tag="ACHU66_000916"
     CDS             9217..10401
                     /gene="dcm"
                     /locus_tag="ACHU66_000916"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFR3389212.1"
                     /translation="MYFFDLFAGIGGFRLGLEQAGHICVGFCEIDRFARNSYQAIFDT
                     EGEVEYHDIRKISNHAFRKLRGKVDLICGGFPCQAFSLAGRRLGFEDTRGTLFFEIAR
                     AAQQIQPQFLLLENVKGLLNHDQGRTFRIILSTLNELGYDAEWQVLDSKYFGVPQHRE
                     RVYIIGHSRRRSRQFLFPIGRKSKVSPLSGPKCMGNINPSGYGLNGKVYSSSAISPTL
                     TTNKGEGVKIAYPILTPERIEKRQKGRRVKQESGPMFTLTSQDRHGVVLEKRLPPSRG
                     VYVRSNTNKGFEAAFEGDSVNFAYPSSKVRRGRVGKQVAQTIVTGNSLGVVTNQLQIR
                     KLTPRECFRLQGFPDWAFNRASRVTSNSQLYKQAGNAVTVPVAFEIGKRLAAIEKEGE
                     SE"
     gene            10398..11357
                     /locus_tag="ACHU66_000917"
     CDS             10398..11357
                     /locus_tag="ACHU66_000917"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFR3389213.1"
                     /translation="MKTLTIVELFGGIGALRKALLNQEIPHQLIDYVEIDRNCVKSYN
                     VLYNENFIPQSVKGYSLPNMQIDLLMHGSPCQDFSRSGLKRGGKRGSYTRSSLLFETI
                     LILKNAVQKPKWVVWENVVGVLDKKMKPTFEAYLTELEYLGYTSCYWILDSFDFGIPQ
                     KRKRIFVVSYLGRENQFDFSKLERTQAPAIEEFLECKVAEKYTVTQPSILKYIEKACQ
                     SIFTARVHIIQEYCYTISTKQTRVPNAGFIRLGTGKYRYLTERECFRLMGFTDKDFNS
                     LRTIFPEKISKKSSILYKQAGNSIVVPVLEAIVKQIYQKELNS"
     gene            11354..11806
                     /locus_tag="ACHU66_000918"
     CDS             11354..11806
                     /locus_tag="ACHU66_000918"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389214.1"
                     /translation="MKLLHQKSIYESNYDEALQHDIEIDNIMSKLFSLPDYNSEFQLR
                     FEDDYHKEMNVPLDYESYLHNIFDFIAEQDIKNGVDVHLTDEGNLCFMAYGQSYTIRS
                     TGVSDVVRTSVTVISKDEAGNQVDFSQHFTIPVQEKEQMNKIKSEQVL"
     gene            12687..13031
                     /locus_tag="ACHU66_000919"
     CDS             12687..13031
                     /locus_tag="ACHU66_000919"
                     /inference="COORDINATES: protein motif:HMM:NF025561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HFR3389215.1"
                     /translation="MIYQQTLHTLQTNPQIQREIGTAPYAKERLEYLSKEESNVEMLL
                     ELYQTAQLIPHLMEVEKEAQTMGVILEQQLIQEWNLTEELKSKDMLKWTAQMNNLRSS
                     IKEQVRYNLIYI"
     gene            14123..19096
                     /locus_tag="ACHU66_000920"
                     /pseudo
     CDS             14123..19096
                     /locus_tag="ACHU66_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
     gene            19093..19731
                     /locus_tag="ACHU66_000921"
     CDS             19093..19731
                     /locus_tag="ACHU66_000921"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR3389216.1"
                     /translation="MNFEEMKSTLENMLNENYADFTKAIIAIEKDISDPDLLQQVYQA
                     YMDDDSISGLLHSNIDAIIETLESDKSQLSTDNVELVGNVTKDVEVKVFPGEDGKEFK
                     VANFSIARNDEQGNSHFTNVTAYNGRADFADQLKKGDFVKLTGQMKYEEYEGKLYKNL
                     VVKTIKILKTKEKQLEEAGKGEKPSTLGALKEYSQHTSSEPSKPKQDKGMEL"
BASE COUNT         6806 a         3152 c         4146 g         5709 t
ORIGIN      
        1 caatattcta tatatttatt tagaatatca tagaaagagg aaaattatgg aacatgaaat
       61 gattaatatt aatgctcatc ttgtcaaaga tgtcattttt tctagctttg aaactgagga
      121 cggtatcgta ccagttgtaa atttctcact agtaaaaaaa tacggaaagg gtaaggaata
      181 cactaattgt tctgcctatg gcgacaaggt agaaattgca aaagaatttg aaaaaggtga
      241 cctcattcat gtgtttggtt actttaagga aaatcgaaaa ggtgataaag tctacaagaa
      301 ttttatagta aaatcgatga ataaaattga aatagaacaa atggaggaag agtaatggat
      361 ttttttacac aaggcgttga tgtcttaaaa acgttggtaa ttgctattgg tggtggttta
      421 ggtgcctggg gtgttatcaa cctcatggaa ggttatggta acgataaccc tggagcaaag
      481 tcacaaggta ttaagcaact aatggctggt ggaggtattg tgttaattgg tttaaaacta
      541 atcccgctac ttgctaactt aatccagtag gaggggctat gtttgatatt tttggaaaac
      601 tagaagaaat gattcaagaa atccttttgg atatgataaa aggaacattg acaagtatgt
      661 ttactgactt gaatcatcaa ttagattttg tatctggcca agtaggtcaa tcgcctagcc
      721 agttcaatgg agaagtgttt tcctttatca aacacattaa tgatagtgtc gtactaccaa
      781 tcgctggatt ggttattaca gctgttttat gtttagagtt aatccaagta gtgatgcaaa
      841 agaacaatat ggcagaagtt gatacgtttg aatttttcaa atatgtcatc aagatgtggg
      901 ttgcagtgta tttagtctcc catgcttttg aatttgcgat ggcagccttt gatgtaggtc
      961 aacacatgat aggacaagca acaggagtca ttcatagtga gacatctatt tcatctgagc
     1021 agattactgc catggttgac agtttgaaag agaagaacat tggggaatta gtaggaatag
     1081 tctttgaact tcaagtagta aaactaacta ttcaaataat ttcattgatt attctcatta
     1141 tcacatatgg aaggatgttt gagatatatg cctatagttc agttgccgct ttgccatttg
     1201 ctacacttgg caatagagat tggggtcaat tgggacataa ctacattcgt gggctgttcg
     1261 cattggcttt acaaggattg ttcctggtca tctgttttgg aatttatgct gtattactaa
     1321 gaggtgtaaa aatgacggat attcatatgt ccgtcttttc aattttaggt tatacagtat
     1381 tacttggatt catgatgttg aaaactggtt cccttgcaag gtcaatcatg aatgcccatt
     1441 agagaggaga taatatggca tatgttcaaa ttccaaagga tttatccaag gtaaaaacaa
     1501 aaatagcttt taatttaacc aagcgtcagt taattggttt ttcggtggca ggcttagcaa
     1561 gtctgcctgt ttatttatct attagggaaa gtgttggtaa cgatttggct atgctcgtct
     1621 ttattgtact cgctttccct tttttattct taacgtttta tgaaaaggat ggattaacta
     1681 gtgaaatcta tcttaagtat attttcttac aacaacacta tcagccatgt gtgagatata
     1741 agagaacact ctacaaaaag atgcacaagg aggaggtaca tgagaccaga aagaaaaaga
     1801 aaacaggcac aagagtttct tgaagcaaag gaagtccaat taaaaaagat tcaaaaggaa
     1861 actctaaaac aggataataa cacgttactt ggttctatta aatcattttt tgaattagga
     1921 gataaatgtt tgactgctga agatacaata ccaattgaaa aagtatatga aaatggaatt
     1981 tttcaattaa ctgatggtta ctatacaaaa atgattgcct ttgaagatat taactatcaa
     2041 ttagcattgg aagagcaacg agatttcatt ttcaatcagt ttgcggcatt tttaaatagt
     2101 tttgataatt cgaccagatt gcaactttgt tttactaacc aacttggaag attggatgaa
     2161 ttagcagatg caattacaat cccatctaaa gatgatggat tagaggctat tcgttgcgaa
     2221 tttagagtta tgcttcaaaa tcaattggag aaggggaata acggcttaag gcaaacaaaa
     2281 tatattgttt ttggtgtaaa agaagatact tatcgtcaag caaaggttgt tttatccagg
     2341 ttggaaattg atctacaatc tcatttgaaa gggatgggga ttcgttctat tgtcctaaat
     2401 ggttttgaac gattgattgt acttcatcat attttgaacc cgacagagca agtttctgaa
     2461 ttgccagagg atttgggcaa tagtgactgt cgctcttcga ttgtgccaga taaatttcac
     2521 tttcagaaga agtatttttc aatggataat gttttgtgtc agaccaatca tgtgcagatt
     2581 ttagcatcag aattatcgga taggttttta actgaattac tttctcttga ggagaatatg
     2641 gttctatcgc tacatataca agcattagat caagttgaag cgatcaagct agttaaacgt
     2701 aagtttaccg atgtgcaacg gatgacgatg gaggagcaga aaaaggccta tcgttctggt
     2761 tatgatttag atattattcc ttccgatttg atgacctatg gtactgattt gaaacaccta
     2821 ttgaatgatt tgcagagtcg tgatgaaaag atgtttctcg tatcgtttat ccttcttaat
     2881 caaggattaa caaaagcaaa actggataat gcagtctctc aattagcaac tatttgtagc
     2941 cgacataatt gtatgctgaa atgtctggat taccaacagg agcaaggatt aatatcagct
     3001 ttaccgttag gaatgaattg tatcaaaatt aagcggcaat taacgacttc aagtacggcc
     3061 gtcttcatac cctttacgac tgaagagctt tttatgactt caagtacatc tatctattat
     3121 ggattaaatg ctataagtca taacttgatt atggcagata ggaagaaact aaagaacccc
     3181 aatggcttaa tcttagggac accaggttct ggaaaatctt tctcagccaa gcgtgaaatt
     3241 tccaatgcca tactagtaac agacgatgat attcttatct gtgatccaga aggagagtat
     3301 ggtgctcttg tagaagcatt tggtggagag gttatccatg tttccgctaa atctaaggat
     3361 tttttgaatc cattagatat aaacttcaac tatggagatg gcgatgcacc tctgaaagat
     3421 aaggcgaatt ttatcatgtc aatgctcgaa cttgtagtag gcggttctgg gttaacagcc
     3481 gctgaaaaat ctgtcattga ccgatgtcta ccgaaaatct acgaagcata cttcgaaaac
     3541 ccaattccag agaatatgcc aatcttacag gatttatatg atttgttgtt aaaacaggag
     3601 gaaagcgttg gtcggaaatt agcgactgaa atggagatat atgtaacagg ttctctaaat
     3661 gtatttaatc atcgttcaaa tgttgacttg aataaatcac ttctatgctt cgatataaaa
     3721 gagttgggga cacaattaaa aaagattggt atgttagtta ttcaagacca ggtatggaac
     3781 aaggtttcac aaaataggca aagtcaaaaa tctacacggt attatattga tgaatttcac
     3841 ttactgctaa aggaggaaca aacagctcaa tattcggttg aaatttggaa gaggtttcgt
     3901 aagtggggag ggattccgac aggtttaact caaaacgtaa aagacctttt agcgagtaag
     3961 gaaatagaaa atgtctttga taatacggac tttatcatga tgttgaacca ggcttcaggg
     4021 gacagagaaa ttctagctag aaaactaaaa atatctccct atcagctcaa ttatgtaacc
     4081 aactctaatg ctggagaggg tctactattc tttggaaata ccattgttcc cttcttagac
     4141 caattcccta agaatacact cttgtatcaa aagatgacaa ccaaaccaga agaggtcagg
     4201 aagggctagg tgaattatgg gaaagtcaaa acgaatggtc cgagagacca ataagaagca
     4261 ttatacaaaa tcaaaacctg acattagtca acggaaggaa gagcaagatt tttctggaag
     4321 aacagttgat gagagtgtta aatttcaaca ccagattatt cataaagaaa gtaaattaga
     4381 gagcaggaaa aatcaagttg aatcttctgg aaagaggcgg aaaaatatta atattgtgga
     4441 tgacaagtct acacaaactc ctttacctat tcatgataaa aatgaatcaa ctattcaaat
     4501 tacagatcta caagatgaaa tagttaataa aaattattta catcagccct ctccaccaga
     4561 agcaccccta gttgaatcaa agccaataag tcataagaaa caagtccaat tacaacaacg
     4621 gaaagccttt gaaaatcagc aaaatgttga atcaaggaac gatattgagt tcaccgagca
     4681 gaatggccat aattctgaaa tacacgttag ttctgatggg gatcttgcac aaactggtaa
     4741 tgaacaagaa gttcaacaaa tacaatttga aattgtgcac cataatccag taaattccag
     4801 cgaactaaat cactcaaagg aattgaattt cggaccaact tcaagtcaaa ctcaggcaca
     4861 aaataattct caaccgactg aatctattga tagaaaaatt catgatactc cgtttcgtaa
     4921 gtttgataca aatcaaagta gaatcattga tactgaaaca aagtttaccc atcggagaaa
     4981 tgaggggaga gcaaaattaa ttcacaatca gaatcaagga acgccgtccc agtttaagaa
     5041 taaaaagggg aagaaacatg ttcatggcga ttcagttcat gaaagtcaaa acattctttc
     5101 ggaattacct atttctcaat ttgattcttc tacagattca gttccaataa aaacttatcc
     5161 aagagtgagt gatgagtcta attctattat tgatcaagca gataatgaga gtgtctttga
     5221 tgtttcactt ctacaaagtc aattgagaaa agataataag aaatttcaac cgagtaattc
     5281 taaactttct aaaaaatcag gtgtcaaaat aatagaggca aaaaatagcc aaagtcaaca
     5341 aattgaaaaa actgtaactc ttacagatgg gggtaaacaa agttcaaacc aatttcagtc
     5401 aaatcttaat gaaaattcga taatgattga acagataagt tatcccaaca tcgactggtc
     5461 taaaaaactt catctacctc aaattaaact attctctaga gatattttgc actctaatgc
     5521 tgtaagcggt aagattcaga atgatgagag taaaccattg accagaaaag gtaagagatt
     5581 atcaaataga ctgagcaata aagcaacaaa atcgaatatt gctcgtagtt tatatcctga
     5641 tacaactagt gacaaaaata gaaaaactgt atctaaatca tccaaaacga aattgagtat
     5701 cactccaatt caagttgatg gaagaccaaa atattatttt gaaactacaa caaaaaaaca
     5761 attttataaa gggcgacctt cttcaaccaa caacattaag gcgaataaat ttagacgtca
     5821 aagattatta aaagatcagt tacaaattca tgaaaagttg aattccttag taattgagcc
     5881 aatagagttt gtaaaggata agaagaatcg tcgtttgctt aaaaaaattg aacaagcaaa
     5941 acttggcaga ataaaatcaa gcccacttaa aaaagtagtg tcaggattgg ccagcagtga
     6001 gtttctagca gcaagttatc ttcaagcagg agctgatgat aatgtggcag ttgatgcaac
     6061 ttcgaaagta ttgaacatca atgctgctat actaatgaga cttgcaaaaa aatcgaatcg
     6121 taatcagacg ataaagaaat tgactaagaa ggctaaggtt agacaatcgc aattggaatt
     6181 tcggcataag tatcgattga tgaaggaaga taagattttt caacggcaat cattctatcg
     6241 aaagtcaatg tatcgacagc ggatgaagca acaaattcgt aacaagcact tccctagaat
     6301 tagagacagg ataaaggaag aactaaaaaa actgttaaat caaatggtac agttcattac
     6361 cacaaagtgg aagagtattt tggtattggc tttggcttta ttctctctat tgacagttgt
     6421 atattcaaat agtcaattta tgtttggagc tttatcatca gtttcttatc agcttaccac
     6481 tacatcctat ctttcttcag aggaaatgct tgttgcattg aatcacatat tctctaccta
     6541 tgaattgaat ttatccaatc aattggcaag attgaaggaa tcgaaacctg gatacgatga
     6601 atacatcgtt aaagggaggg aacaaattgg tcatgatcca catgtcttat tagcctattt
     6661 gacagctaga tttggggaaa taaaagacca aacaagtgtt gaaccagcga tgaaacaact
     6721 atttgttcag atatatcagg tttcctatcg tgaagagagg gaaatccgtt atcgaaaggt
     6781 caaggaagaa gttcttgaca ctagcggtaa gaaaacgatt gttgagaaag aagttcccta
     6841 tacctatcga aaattgatag caacagtttc aaaaaatgac atggatgtag ttattcagtc
     6901 aatatttgcc ggcagtccta gcaatttaga acattatagg attcttaggg aaacaaaagg
     6961 gaatttagaa actgcctttc cttcgggaac gacgatcagt cttcctggaa atgtatcatc
     7021 ggtatatgat gtcaatttaa caggcggaaa ttttccacca ccaaacccta atcatgttgc
     7081 tgctctaaat ggcggttacc ctgggcaatg tacttggtat gtttataatc gattctcaca
     7141 acttggaaag ccaatccaac attcaccaat gggaaatggt ggcgagtggg cgttctatgc
     7201 tgctaaatac ggttatccag tcagtagaga ggcacgagct ggtacagcag tctgtatgcc
     7261 tccaactgta ccatatgcag atccgactta cggtcacatt gcgtttgtag agcgcgttaa
     7321 ccctgatggt agtattgtta tctcagaaat gaatgtaaaa ggggaatttg tgatttcaac
     7381 aggctatcta ccacgtgaga tggcggcaca atgttattac attaattttg gattataagg
     7441 agaaatatat gcgtaaagat attcaaatgt tacaaaaaaa gaaagaaaaa ttacttgaaa
     7501 aaaagcaaaa aattgaccaa gatttggctc aagttttaca agaagaacaa gatttgattt
     7561 atgaagaagt catagttgtt tttgagaaat ctaatctatc cctcgaagaa tttactaaaa
     7621 aagttttatt tgaaaaggag ttgtcgcatg aaacgattat ttagttttgt gagagtagct
     7681 agtttggtta tgttatttag cgggtgccaa actatttatg caagttcacc tagttcagag
     7741 ccaaatagtc agccagtagc aatttctgta catccacaag cctcagagca aagcaacgcc
     7801 aagggccaag tcacagaaaa tgtagatgcc aataatcaag attatgaaat tactgattca
     7861 gttgctacta agcggcagtt tgtcactttt acaagtaagt caggaaaggt gtttcacctg
     7921 attatagatc atgacaaagg tggacaaaat gtacaattat tgacagaagt atctgaacag
     7981 gatttactta accttgttga atcaactgat actgttgcag tcaaaccaca aaaggttgaa
     8041 gaagtcgttg atgataaacc tgtaaaaaat gaagaaccaa aacaaaaatc ttctgctgga
     8101 agttacatta ttatcggtct atttctcctt ggtgttttgt gtgtaggata ttatataaaa
     8161 gtcattaaac caaagaaaga acaccacttt gaagaatttg aagaagatga tgactatata
     8221 agtgatggtg aagaagaggt ttgacattgt tttccttttt tgtataatga aattagaggt
     8281 aatgaaatgg atttatcaag taagcaaatt aagaatttat cacataataa atggtaccat
     8341 gctacattaa aacgtcatct ctcttcctta ttagaaggga taaaagtaga ctacaattta
     8401 ggacatgaac tggattttgg tgctggtttt tatattacat cagattttca acaggcacag
     8461 aagtatataa accgtttggt agaattttta aataccaata aagctggtgc tgaatttttg
     8521 gggaatatgg atgaggaagg gattattcta gagtttgagt tgacagattt tccttcaatt
     8581 tttacaacat caaattataa gtgtcattat ttcaaaagcc acaatatttc tgatgatgtg
     8641 atagatttcg ctgagttcgt ttttcagaat cggcttcgct caaaggaacg cattcatgat
     8701 ttcgatttta tttatggagt tcagacggat gacaatccaa ctggtttact ccaaaaatat
     8761 aaggctggat tggtttcaaa agctgatgta ttgaacgaat ttcgtaagcc gttctctttt
     8821 aaacagttat ccattcacaa tcaagagttt tgtgatataa tgaaggtaac aaagatttat
     8881 ttatcaaaag atggaaagga gttagaccaa tggcaaaaac aatgattagt cctgttgaat
     8941 tgtatagcaa tgagttagca caggctttgt tagaaacatc aaaatataga cttgaggcta
     9001 gtgttgcgca tcagatagca agacaatatg caagtcaggt agattttgaa gatccaattt
     9061 tgatgcacgt tggagttaac tccattgcaa gcaccttgat tgacaaaatt aaacctgaat
     9121 attttcaaac aacttcataa agtttttaaa cttgaatgat gattttagcg attagagaaa
     9181 tctagtcgct tttttctttg tcaaaaagga ggataaatgt atttttttga tttatttgct
     9241 gggataggag gttttcgatt aggtcttgaa caagctggac atatttgtgt tggcttttgt
     9301 gagatagaca gattcgctcg taactcctat caggctattt ttgatacaga aggagaagtc
     9361 gaatatcatg acatacgaaa aatatccaat catgctttcc gaaagctccg aggaaaagtt
     9421 gatctcatct gtggtggatt cccttgccag gcattttcgc ttgcaggaag aagattggga
     9481 tttgaggata ctagaggaac tttgttcttc gagattgctc gagcggccca acaaatccaa
     9541 ccacagtttt tattgcttga aaacgtcaaa ggcttactca atcatgacca agggaggacg
     9601 ttcagaatta tcctttccac gcttaatgaa ttggggtatg atgctgaatg gcaggtactt
     9661 gacagcaaat attttggtgt ccctcaacac agggaacgag tctatattat tggacattct
     9721 agaagacgaa gtagacagtt cctttttcct atcggacgaa aaagtaaagt ctctcctcta
     9781 tcaggtccaa aatgcatggg aaacataaat ccatcgggat atggattgaa tggaaaggtg
     9841 tattcttcta gtgctatttc tcccactctt acaacgaata aaggcgaggg cgttaagatt
     9901 gcttatccaa ttctaactcc tgaaagaatt gagaaaagac agaaaggtag gagggtaaaa
     9961 caagaatcag gacccatgtt tacacttact tctcaggaca ggcacggtgt tgttttagaa
    10021 aaacgtttac ctccgagtag gggagtttat gtacgctcaa ataccaataa gggatttgaa
    10081 gctgcatttg aaggagattc tgtcaatttc gcttatccgt catctaaagt tagaagaggt
    10141 cgagttggta agcaggttgc tcaaacaata gttactggaa actctcttgg ggttgtaacg
    10201 aatcaattac agattcgtaa gctaactcca agggagtgtt ttcgtttaca gggatttcct
    10261 gattgggcat ttaatcgagc aagtagagta acttcaaatt cacaattgta taagcaagca
    10321 ggaaatgcag tgactgtacc tgttgcattt gaaattggca aacgtttagc agcaattgag
    10381 aaggaggggg agagtgaatg aagactctga caattgttga attattcgga ggaataggag
    10441 ccctccgaaa agcacttctt aatcaggaaa ttcctcatca attgattgac tatgtagaaa
    10501 tagataggaa ttgtgtaaag agctacaacg tactctataa tgagaacttt attccacagt
    10561 ctgttaaagg gtattctcta cccaatatgc agattgactt gttaatgcat ggcagtcctt
    10621 gtcaagattt ttcacgctca ggacttaaaa gaggtgggaa gagaggctcc tatactagaa
    10681 gttctctctt gtttgaaaca atcttgatat taaaaaatgc ggttcaaaaa cctaaatggg
    10741 ttgtttggga gaatgttgtt ggcgtgttag ataagaaaat gaaaccaaca tttgaagcct
    10801 atctaacgga attggaatat ttaggctata catcgtgtta ctggatactg gatagctttg
    10861 actttgggat acctcaaaaa aggaaacgga tttttgtggt cagttatcta ggtagggaaa
    10921 atcaatttga tttttctaaa ctagagcgaa cacaagcacc agctattgaa gaatttttag
    10981 aatgcaaggt agctgaaaaa tatactgtca ctcaaccgtc cattcttaag tacatagaaa
    11041 aggcatgtca gtcaattttc acagcacgag ttcacattat tcaagaatat tgttatacga
    11101 tttcgactaa gcaaacacga gtacctaatg ctggatttat taggctcggt actggaaaat
    11161 atcgttactt aacagaaaga gaatgtttcc gattgatggg atttactgac aaggatttta
    11221 attcattacg aacaattttt ccagagaaaa ttagtaagaa atccagtatc ttgtacaagc
    11281 aagcaggcaa ttctattgta gtaccggtac ttgaagcaat tgtaaaacaa atttatcaga
    11341 aggagttaaa ctcatgaaac tattacatca aaaatcaatt tacgaaagta attatgatga
    11401 agccttacaa catgacatag aaattgataa tattatgtca aaactgtttt cacttccaga
    11461 ttataattct gagtttcaac tgaggtttga ggatgattat cataaggaaa tgaacgttcc
    11521 acttgattat gaatcatatc tccataacat cttcgatttt atagcagaac aagatattaa
    11581 gaatggggta gacgttcatt tgactgatga aggaaatcta tgttttatgg cttacggtca
    11641 atcttacacg attcgctcta ctggagtgtc agatgtggta cgaacttctg tcacagtcat
    11701 ctctaaagat gaagcgggaa accaggtgga ctttagtcag cactttacta ttccagtaca
    11761 ggaaaaagag cagatgaaca aaatcaagtc agaacaggtt ttatagcgaa agaaaggaga
    11821 agtcatgcga gttgaagaat atgccaacta tgtcggagac ttatccactc attatctttc
    11881 agatgtagat gcttatcaaa ggatgctagg gcggctaggt tatgtcgttg gttttgactt
    11941 tcaaaaccaa ttgagtatta ttcatcagaa accgacagct agcttatgct cagattttca
    12001 atcttggaag tccgttgact atattgttag acgtggtcaa aaaggggtac cgatattatt
    12061 atctaatcaa tcaacgacta gcgtagggta tgtctttgat attagccaga cagtcctagt
    12121 gaataaggat agacgaccaa ttgaacaatg gagatatgaa tcaggaaaag atttatccat
    12181 ccttacagat attattgatg agagattgtc acttagaccg gaaaatgaag aggcagcgat
    12241 tcgttatttg gcaggattag ctaccaaagg cattggtcca aaagttctag caggaatgaa
    12301 tttagatgtg gaaagtgaaa actatttact tcgttttatt gaacaaagtt ttcaagcagt
    12361 atttgctggt agacttgggg ttgagatggt cctaaacaat gatttacttg agaatgggtt
    12421 aggtcaattt agtcctagac agtttctgct ttttggtgat tacttatcaa gaatgtccca
    12481 aaaaattttt cgtattattc accaaaaaaa gattgagaaa attgcattaa aagagcagac
    12541 aaaagagatt attgagcggt ataatgatgg tgtagaagat agaggaggtc aaccaaatga
    12601 atcaagcatt agttatcgag aagatgaata cgaccatgac agccaacgag ttcagtcatt
    12661 gtcagacaac agaggagata gtagagatga tttatcaaca aactcttcat acacttcaaa
    12721 ccaatccaca gattcagaga gagattggga cagctcccta cgccaaggaa cgattggaat
    12781 atctgagcaa ggaggagagc aatgtggaaa tgttgctaga attgtatcag acagcccagt
    12841 tgattcctca cttgatggaa gtagagaaag aggctcagac gatgggggta atactcgaac
    12901 agcaattgat acaagagtgg aatttgaccg aggaattgaa gtccaaggac atgctgaagt
    12961 ggacagctca gatgaacaac ctacgctcct cgatcaagga acaggtaagg tacaatctga
    13021 tttacattta gtaaaaataa ctaaagaaga attgcatgat gttcttcgta aaggaacagg
    13081 aacacaaggt gggcgactaa gagttgctca cttttttgct agtcaagaag atataaaatt
    13141 tcaagcccag tttttacaaa aggaatacgg ctggggtgga gcaagtggtc caagttttcc
    13201 aatttcctat caatatgatt caaagggatt acatttaaca cgtaaatccg atggacatag
    13261 ggaatcattc agttggcgac aagttgcggg tgaaatagat gctctattgg aagaagaaac
    13321 ctatctttct gatgatgaaa ataaacttta tcgttttaat gaccatattt tttctgactt
    13381 tgagtcctca ccattatttg aatttgataa acaagaaaat ggacaaatca tcgtctacat
    13441 ggaagatgaa atggttgctc gcattgatac ggttggtcag attaactatg tactggaatt
    13501 agaagaacga ttcagatacg aaattgatga gtatgcacga aacttagaaa acagtctatt
    13561 gcttgaaaaa atgaaggagc atgagcaatt agacctcttt gattttgatg ccttaggaga
    13621 ggaaacatct gatgaaactg cagaagtaga acacgagaat tctgaaaaat acagaattgc
    13681 agagtccacc acagttaaga ctgaagaaag accattgaat aattttgtac ttgaagatct
    13741 accaggttca ttacctccgt cacaacgctt agaaaataat cttgccgcaa ttcaaatcat
    13801 aaagaatatt gagacagaag gcaggagagc caaccgacaa gaacaagaaa ctctttcatt
    13861 atatgttggt tggggaggtc ttgcagatgt cttcaatgag gagaagacag gtcaatgggc
    13921 tgatgctcga agtaaattac aagaattatt atcaccagaa gaatatcaag cagccaagga
    13981 atcaactttg actgcttttt atactccacc actcgtgatt gattctattt ataaagtctt
    14041 gcagaatctt ggttttgaga aaggcaatat tctggaaccc tcttgtggtg tagggaattt
    14101 tatcggtcgt ctaccaaaag atatgaatcg ttctaaggtg tatggagttg agctggataa
    14161 gttgagtggt aggatagccc aggaacttta tcaaaaatca aatgtgcaga taaagggatt
    14221 tgaagaaaca agattttcaa ataatttctt tgatgtagcg gttgggaatg tgccgtttgg
    14281 agatttcaaa ctaactgatc gagattatga ccgagaaaac tttcttatcc atgactactt
    14341 ttttgccaag tcaatcgaca aaattcgtag tggaggtatt attgcatttg taacttcttc
    14401 tggaacaatg gataaacaag acagtcgtgt tcgacaatat ttggccaatc gatgtgactt
    14461 gttgggtgct attcgattgc caaatgatac ctttaagggg ttagcaggta ctgaggttac
    14521 aagtgatatt ctagtcttac aaaaaaggga tgttgtgaga gagcaggatg tggactggat
    14581 acatttagct agagatgaga atggccttac ttacaataag tattttgtag agcatccaga
    14641 gatggtcttg gggcaaatgg cagaagtctc aggtcgattt ggtcctagtt tagcctgtct
    14701 ttcagatggg agtgacctac aggagcaact tagcgcagct attcaatcca ttcagggaga
    14761 aatcccacgt gccgaacaag ttcacaattt agatgatgga caggacattc ttccagcaat
    14821 ggatcatgtt aagaatttct cttatacttt ggttgatgga gttccatatt acagagaaga
    14881 ctctgtcatg attcgtcaaa agttatcact taaagaacta aatagattaa gagcttacat
    14941 ctacgtagtg gatagtttga aaaaagttat tcaactccaa aaagaaaatg ccagcgatgt
    15001 tttgattcaa gaaagtcaag agcaactgaa caaggcatat gattctttta aagcgaaata
    15061 tgattttatc aactctacac ttaatcgcag gaacttacgg gaggatagtc attatccctt
    15121 agtatcttct attgagaagc tagacaaagg taagtttgta gggaagggcg atattttctt
    15181 taagcggaca attgcaaaag caaaggttgt cgagcattgt gatactagtc atgaggcctt
    15241 gattctttca atttctgaaa agggaagagt tgacttcgac tatatgacac agttgactga
    15301 caagcccagg gaggaattga taaaagaatt aaaaggggaa atctatttgg atattccttt
    15361 tgcctatgag gagagtatgc ttcatccatt ttctgagcaa gggatggaag aaatgggatt
    15421 atcttatgtt cctgctgacc aattcttaag cggtaacatc cgtgagaaga tttctataat
    15481 ggatttttac attgataggt atcaatcaat cgacaatcca gataaggaag ttcttgagaa
    15541 tattaacgaa ctaaaatttc aacgtgatag acttgaagaa gttatgccca aggaattgac
    15601 agcaagtgaa atttcagtcc gactaggggc tacctggatt ccaacttcag atattgagca
    15661 gtttatcttt gatacgcttc aacctagcta ctatgcgaaa aaagcaatta aagtacagtt
    15721 ctctccctat acatcggaat ggaagattga tgggaaaaca caggatagtc gaaatgacct
    15781 tgccaatctg acttttggta caagtcgagt caatgcctat cggttaatcg aggattcact
    15841 aaatttacga gatacacgag tctacgatac agtttatgat gatgcaggaa ataaaaaaca
    15901 agtcctgaac aagaaagaaa ccatgttggc cggtgaaaag caggaacttc tcaaagaatc
    15961 tttcaaagac tggattttca aagaccaaag tcgtaggcat aggcttgaaa aactctacaa
    16021 tgaaaaattt aactcaatcc gaaaccgaga atatgatggc caacacctct ctcttgaggg
    16081 gatgacatca gatattagcc tacgtccaca ccaaaagaat gccatagcac gaacgcttta
    16141 tggaggaaat acccttttag ctcatgtagt cggagcaggt aaaacgtttg agatggtagc
    16201 aagtactatg gaaagtaagc gacttgggat gagtaataaa gccctgtttg ttgttccaaa
    16261 ccatttgaca gaacagatgg gaagggaatt tatggagttg tatccagcag ccaatattat
    16321 ggtagccact aaaaaagatt ttgaacctgc taatcgaaaa cggtttgtag gtaggatagc
    16381 gactggcgaa tacgacgcgg tcattattgg tcacactcaa ttcgagaaga ttccaatgag
    16441 tagagaatac caggaacaac atctgaaaca tcaaattcag gatatagtcg agttcattga
    16501 agaatataag tatgagagag accaaaactt tactgtaaaa cagcttgagg ggacgaagaa
    16561 gaaactagag gcaaagctaa aaaaactaaa tgacgatttt cgtaaggatg atgttgtgac
    16621 tttcgaggaa ttgggtgttg ataaactcta cattgatgaa gcccataact ataagaatct
    16681 ttatctttac accaaaatga gaaatgtggc tgggattggt caatctgaag ccatgaaatc
    16741 ttcggatatg tttatgaagt gtcgctattt ggatgaatta acaggaggaa agggtgtcgt
    16801 ttttgcgaca ggaacacctg tgtcgaattc tatgagcgaa ctttacacca tgcaacgata
    16861 tctacaattt gatgaactgg aacgaaaagg gctacatcat tttgattcct aggcttcgac
    16921 ctttggcgaa accgtaactg ccatagaact ttctcctgaa ggagactcct atcgtagtaa
    16981 gacacgcttc tcaaagtttt acaatcttcc tgaactgatg tctcttgtaa aagagatggc
    17041 agatattaag actgcagatc agttgaattt accaactcca caggctcatt atgaaaccat
    17101 acttaccaag ccaacaggtc atcagaaaga aattttaaaa agcttttccg agcgtgctga
    17161 taaggtgaga aataggaaag tcgagcccca ggaagataat atgttgcgga ttacgaatga
    17221 tggtaagaag attgctttag accagcgttt ggttaattcc tttttaccag atgatccaga
    17281 gagcaaggta aatgtctgta ccaagaacat tttcgcaatc tgggaacaat caagtgagcg
    17341 acgttccgct cagctcgtat tttgtgacat gtctacaccg aagggggatg gaagttttaa
    17401 cctctacgat gatatgaaaa ccaagttagt taatcttgga attccagaaa atgaaatcgc
    17461 ctttattcat gatgcgaata atgaaaagca gaaagctgag ttgtttgcca aggtgagaag
    17521 tggcgatatt cgtattcttt ttggttccac atcgaagatg ggggcaggta caaacgtgca
    17581 aagcaaactc attgccctcc acgacttgga tgtaccctgg agaccagcag atttggagca
    17641 acggtctggt cggatcgttc gtcagggaaa tgagaataag gacgtctata tctatcgtta
    17701 tgtgacggag aatacattcg acgcatatct ttggcagacc attgagaata aacagaagtt
    17761 tatctctcaa attatgacct cgaaaacgcc tgtgcgtgtg gctgatgatg tggatgagaa
    17821 taccttaaat tatgcggaaa tcaaggcact tgcgactggt aatccactta tcaaagagaa
    17881 gatggattta gatgttgaag tatctaaatt acggatgctg gaatcaaact acaagtccaa
    17941 tttatatcag ttagaagata agatagcaaa acactatccg ttcgaaatcg ctcgtctaac
    18001 agaacaaatt gcatttgcca agcaagatat ggagagaaag gtttcaaaag tacaaggtga
    18061 cggtcaattt aatggtatgg aaattttagg aaaacacttt gtagataaga aatcagcagc
    18121 agaagaatta atgaaaattt taccactagt tcctgcatca ggtacaatgt caattggtca
    18181 ctatcgtgga tttgaagttt ccgcattctt taaccaaatt gctcaagcaa tggattttgt
    18241 gattgagggt aatttatcct acgttggtca tttcggtgat agccctgagg gcaatattca
    18301 acgtatagac aatgttttgg ataggataga tgaagaagta tccaagttgg aaaatagact
    18361 gtcagaggtc caatacaagc tccaggttgc gaaagaagag gtaacgaaac cttttgaaaa
    18421 ggcggactta ctaaaagaaa agactttgcg tttagcagag gtcaatcgca tcctggatat
    18481 gggggatgtg gaagagttag agaatccgaa tcctttactt gaagatgtta aggcagcaat
    18541 tgttgacttc ttagccagag aatatggaga tacagaactt tctgaagaaa gtagaacgga
    18601 acaatttaaa gacatctttt cgactggaga tgtggtgtat ctagcaaagg gaaaaagcga
    18661 ggttggagac tatgatattg agtatagtgt tgaactgtcc aatttttcct ggaaattatc
    18721 ctttgcggat tcagatataa tgtcaggcca gtttgaagga agtacaccag aggagcaagc
    18781 tcaacacttt aaactatttt tggagcattc caccaatgag gacttactct caattgatga
    18841 aaaggaggct tgtcaaacgc taggtttacc ttatactaga gatttgcttg acaaggattt
    18901 ggataatgat ggtatcgtag accgatatga ccatgatttt aaagatagtg atgcttttga
    18961 atcaaactat gatgtcgacg agaaagaaaa acggccatct actttagcac aaattcaagc
    19021 ctataaggca gaagttgaag aacgtgacta caaaagccaa acaaaagaag aaatagaaga
    19081 ggagcaagta atatgaattt tgaagaaatg aaatctactt tagagaatat gttaaatgag
    19141 aattatgcgg actttactaa ggccattatt gcgattgaga aagatatttc agatccagac
    19201 ttgttgcagc aagtctatca agcatacatg gatgatgatt ctatatctgg tttacttcat
    19261 tccaatattg atgcaataat tgaaactttg gagtccgata aaagtcaatt atcaacagat
    19321 aatgtagaat tggttggcaa tgtaactaaa gatgtcgagg ttaaagtctt ccctggagaa
    19381 gatggaaagg aatttaaagt tgccaatttt tcaattgctc gaaatgatga acaagggaat
    19441 agtcacttta caaatgtgac agcttataac ggacgtgccg actttgcaga ccaactcaag
    19501 aaaggggatt ttgtcaagct aactggccag atgaagtatg aagagtatga agggaaactc
    19561 tacaaaaatc ttgttgttaa aactattaaa atccttaaaa caaaagaaaa acaactagag
    19621 gaggcaggaa aaggagaaaa gccctcaact ctcggagctt tgaaggaata ctcccaacat
    19681 acaagttcag aaccaagtaa accaaaacag gataaaggga tggagttgtg acaaaaagct
    19741 agaccaccat tttcggaggt ctagcttgtt tttttattta ctctaaactt gttgtcgttt
    19801 tctgatatat gaa
//
LOCUS       DAZQCJ010000028        19731 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000028 DAZQCJ010000000
VERSION     DAZQCJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19731
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.028"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..570
                     /locus_tag="ACHU66_000922"
     CDS             <1..570
                     /locus_tag="ACHU66_000922"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR3389217.1"
                     /translation="NLFCVNFYYKNRKEYYMIIWLNGPFGIGKTTLANILHKRIENSY
                     LYDPELLGDFLQHQLPKTVCPEDFQDYSVWRRSTYKIVFDLATKTDKIIIIPMTIYKK
                     EYYQEITQQLIQDKIPLEHYILLADKTTILERLDNRVNEDNIWAKRHLDVCLKAFESH
                     IPGQRLNTDSLKPEEVAEEILMLSEFTKK"
     gene            737..1807
                     /gene="xerS"
                     /locus_tag="ACHU66_000923"
     CDS             737..1807
                     /gene="xerS"
                     /locus_tag="ACHU66_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFR3389218.1"
                     /translation="MRRELLLEKIDQLKEIMPWFVLEYYQSKLAVPYSFTTLYEYLKE
                     YRRFFEWLQDSDLVSVERIADIPLDILEHLTKKDMEAFILYLRERPLLNANTTQNGVS
                     QTTINRTLSALSSLFKYLTEEVENEQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETMEFLDYVDKEYQVNLSKRALSSFQKNKERDLAILALLLASGVRLSEAVNLD
                     LRDVNLNMMIIEVTRKGGKRDSVNVAGFAKPYLEAYMSIRQQRYKAEKTDTAFFLSEY
                     RGLPNRIDASSIEKMVAKYSADFKIRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAN
                     TQVTDLYTHIVNDEQKNALDQL"
     gene            complement(1877..3385)
                     /locus_tag="ACHU66_000924"
     CDS             complement(1877..3385)
                     /locus_tag="ACHU66_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFR3389219.1"
                     /translation="MSHHHVLQNGSDIRGIAIATDEYAVNLTPQATQEVVRGLIHWLT
                     QKPELAQTYQKGQLTIGIGRDSRLSGPDLVAAFTEEAVRLGVQLIDFGMATTPALFMS
                     TQYPQFKCHAGVMITASHLPHYFNGIKIFSENGGAEHEDIDFILSSSEDLPASDLGSV
                     TKADLITPYAQDLVGKIRTACGGQEKPLTGLNIIVDAGNGAGGFFAEKVLAELGADTT
                     GSQFLDPDGTFPNHVPNPDNKEAMESIRQAVLKQGADLGIIFDTDVDRAALVTKSGEI
                     LNRNNLIAVLSQIVLAEHPGTSIVTNSPTTEHLKVFIESLGGKQIRYISGYRNVINRA
                     ILANQEGVDCQLAIETSGHAAFKENYFLDDGTYVAAKILMLLPKLQAEGKSLDDLIAQ
                     LKQPLETQEVRFKLEAAGYRALGEQVIADFRNTSLEGFAFNPENEEGVRFDLTELYGD
                     GWLLLRMSLHEPLLVLQVENDQTGHIPAVLRTLSAFLDQYPAVNQEKLKALI"
     gene            complement(3461..4402)
                     /locus_tag="ACHU66_000925"
     CDS             complement(3461..4402)
                     /locus_tag="ACHU66_000925"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR3389220.1"
                     /translation="MIAKFIREYRSFYFSYIVLATSFILLFFLYQLPTEFLQNALLLS
                     LTLLILLTVGLFWKFRNRMRALEDYVHEEALPLLNKPVDLAYLNLIEAEKEATREQLL
                     VYKSREEDLQAMVKMWSHQMKVPLAALSLMSQTDNLNQQDVNHQLLRLDNYMANLLNY
                     LKLTNHASDFRFEQVEVREVVVDLVKKYRNQFLQKDISVTIDGNWLLKSDRKWLSFAL
                     SQIIDNALKYNKTGRKVAIELNEGISISDTGIGILAEDIPRLFDEGFTGFNGREHQKA
                     TGFGLYLTKRVLNQLELAISVESQLEVGTSVTITKVQ"
     gene            complement(4399..5064)
                     /locus_tag="ACHU66_000926"
     CDS             complement(4399..5064)
                     /locus_tag="ACHU66_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFR3389221.1"
                     /translation="MKKEKIYIVEDDEMIVQLLKQHLGKSYQVESVQNFRAVSQEVAE
                     IKPDLVLMDISLPYYNGFYWTTEIRKTMTMPIIFISSSDDEMNAVMAMNMGGDDFVSK
                     PFSLGILDAKIGAFLRRVNQFSKSTDLSIDQFQLSLDGRFSNELEQVQLSPTETKILS
                     ALLERQGQIVSKEELLEKLWENEEFIDQNTLNVNMTRLRKKVSEVGFERIHTVRGVGY
                     LVK"
     gene            complement(5103..5360)
                     /locus_tag="ACHU66_000927"
     CDS             complement(5103..5360)
                     /locus_tag="ACHU66_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR3389222.1"
                     /translation="MSYKVILSAKAQKQIRKMDKTAASLILRYLYKHIDGCDNPRQYG
                     KALTANRSGQWRYRIGNYRVIVNIEDDKLIVTSIKMRNGSI"
     gene            complement(5350..5589)
                     /gene="relB"
                     /locus_tag="ACHU66_000928"
     CDS             complement(5350..5589)
                     /gene="relB"
                     /locus_tag="ACHU66_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFR3389223.1"
                     /translation="MAAITLKVSEQDKLFMQAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADVAWAEYQADLANRIEPMTLEQMAEELGIEL"
     gene            complement(5681..7651)
                     /locus_tag="ACHU66_000929"
     CDS             complement(5681..7651)
                     /locus_tag="ACHU66_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFR3389224.1"
                     /translation="MFYLKLAGQNIRKSIGVFAPFVLASLVLFTLICSMFLLMLSPVM
                     KTMGTGAVSIGLGVVVLTIFSVIMEIYSFNFLMKQRAREFGLYNMLGMNKKKVALIAS
                     IELMMIFLLVVVLGSVLAGVFANVLYLIFVNITHYSDLHFGIEPLAFVMTAFLFAGFF
                     FLLELLAIRTIGKTSPLILFRASEKGEREPKGNVLLAALGVLSLGMGYYLSLSSKAEG
                     LAVIYRFFIAVLFVIAGTYLFYISFMTWYLKARRKNKRYFYTPEHFITTSQMIFRMKQ
                     HATGLANITLLAVMAFVTIATTTSLYTGMANMTSGLYPKETSITYKVSYRSQGVAAYQ
                     QSVLSHFPEKANDNLSYLTYQAGLVYDGGSDIVINPETIANPDFSKMAFTYFITQDDF
                     RKLGNDLPELAANQVAFMRPSEMPSLKKLVLGDQTFENVANLDQAIFPDITNTLNAAV
                     MVVSDDTVLDAVREYYESNNPQGYTVSLDYRVFTDLTDEEVASIGQQTGDSYNINDAN
                     GEFLGYAMQKSDFNNLLMGFTGSFLFTGFLLGISFLLGAALIIYYKQYSEGHEDKKSY
                     KILQEVGMSQQAVKKTINSQTLLVFFMPLAMATLHFIVALVMLKQMLMVFGVVSSSMI
                     YTVSGITLLAVVLIYFVIYKWTSRTYYRIIER"
     gene            complement(7652..8404)
                     /locus_tag="ACHU66_000930"
     CDS             complement(7652..8404)
                     /locus_tag="ACHU66_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3389225.1"
                     /translation="MLLEINHLEKVYRTRFSKEETRALQDVDFKVEEGEFIAIMGESG
                     SGKTTLLNILATLDKPTRGSVILNNQDITKIKESKLADFRLRNLGFVFQDFNLLDTLS
                     IQDNIFLPLVLARESYEEMEKRLKVLAPKLRIQDLLKKRPFELSGGQKQRVAIARSLI
                     TQPQIVLADEPTAALDYRNSEDLLNLFEDINLDGQTILMVTHSANAASHAKRVLFIKD
                     GRIFHQLYKAGKSNQDFAKEISLNMSALLGGE"
     gene            complement(8421..8804)
                     /locus_tag="ACHU66_000931"
     CDS             complement(8421..8804)
                     /locus_tag="ACHU66_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NisI/SpaI family lantibiotic immunity
                     lipoprotein"
                     /protein_id="HFR3389226.1"
                     /translation="MKKKVFGLLALGSIILTACSFSSAGLSFDEGYQTFHYKGKDYAV
                     SKQEVAENNIQEVEVKFMEWPVVKEEGAVKKTVSLNNLYITTSKEWAIGVQDGYYKVD
                     LEDNIAESDRIDYQAVLDQVDLDSE"
     gene            complement(8817..8957)
                     /locus_tag="ACHU66_000932"
     CDS             complement(8817..8957)
                     /locus_tag="ACHU66_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389227.1"
                     /translation="MSFKFLQVKRMEEKAFTEKEKTELLEQAEFLEELALDLQTMRFM
                     AY"
     gene            complement(9056..9790)
                     /locus_tag="ACHU66_000933"
     CDS             complement(9056..9790)
                     /locus_tag="ACHU66_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HFR3389228.1"
                     /translation="MKSYNAYQLKLAMAGLMVLDHLKIIPDFIPDQLALVFHLATRCV
                     AVFFAYMLVEGFLHTRNVKAYLLRLYGAAGLMATGNTLLNSLYQSKGIYISNNIFLTL
                     AVGLTLLICLQNFSEHTRAKQIIQVLLAGLLTIIGALFTEGGTVVLPFILITYLGRES
                     ATKRTIAYGFLALVLLAMSYQPYERLEMTIQMMLYNADWLFILVLPILSLYNGQAGPR
                     TVFSRYFFYIFYPLHLWILATIGYFL"
     gene            complement(9803..9940)
                     /locus_tag="ACHU66_000934"
     CDS             complement(9803..9940)
                     /locus_tag="ACHU66_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389229.1"
                     /translation="MSFKFLQAKQIFGKSQESKRLEEQDKQAEFLFELEHELFQRNWM
                     Y"
     gene            complement(10079..11245)
                     /locus_tag="ACHU66_000935"
     CDS             complement(10079..11245)
                     /locus_tag="ACHU66_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit family protein"
                     /protein_id="HFR3389230.1"
                     /translation="MNYLVISPFYPENFQPFTIELAKKEGVTVLGIGQEPYDQLPEEL
                     RNALTEYFRVEDLENIDEVKRAAAFLIYKHGPIDRVESHNEYWLENDAALREQFNIFG
                     AKPKHLKKTKFKSEMKKYFKKAGVPVVPGVVVKSERDFGRALKNVGFPMIAKPDNGVG
                     ASGTFKIENKEDFDRFVETWDGATIYFLEKFVNSSIITTYDGLLDHEGNVVFETGLTY
                     VHTPLELMLSRKDNAYFIEKELDPQLVKYGRAIIKAFGMRERFFHIEFFKDGDDYIAI
                     EYNNRMAGGFTVEAYNYAHSIDLFRDYANVATGGTVEERRFEPQYCLVATRRDTTEYV
                     HSADDIHQKFAGKIKTVKRMPDAFAELQGNDAYLLVTESKKELDEMIAYIGLTK"
     gene            complement(11264..12010)
                     /locus_tag="ACHU66_000936"
     CDS             complement(11264..12010)
                     /locus_tag="ACHU66_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002909732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFR3389231.1"
                     /translation="MHVEFLSHWSGNLGREMNLNRYGHAGIPVVVFASSGGSYNEYAD
                     FGMIEACRGSIEAGQVQFFTLTSYDSESWLADWKSIHDKAEAHRAYERYVIEEAIPFI
                     KHKTGWFDGMMTTGCSMGAYHALNFFLQHPDVFTKVIALSGVYDARFFNNNHDYGHDD
                     AVYQNSPSDYIWNQNDGWFIDKYRQADIIVCTGLGDWEQDGLDSFYNLKRAFEEKNIP
                     AWFDTWGTDVAHDWVWWRKQMPFFLHSIGL"
     gene            complement(12041..12868)
                     /locus_tag="ACHU66_000937"
     CDS             complement(12041..12868)
                     /locus_tag="ACHU66_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR3389232.1"
                     /translation="MNKSYFYLDMRTHELDVPFTGRKRRVRVLLPKGYAENTEETYPV
                     VYFHDGQNVLYSKEAFSGHSWKVIPTIKRNPDIAKMIVVAIDNDGADRMHEYAAWKFS
                     EMGIPGVQFGGKGTLYAEFVMDVVKPFIDKEYRTKSDKVHTAMIGSSLGGNITQFMGL
                     AYQNQIGCLGVFSSANWLHQDAFDRYIERQQLDSEQRVYIYVGTEEADDTDKTLMAGN
                     IKQAYINSSLTYYRQLIAGGVDLDNIALEVVSGAVHNEEAWALYLPACLRFLSEHWS"
     gene            complement(12982..13986)
                     /locus_tag="ACHU66_000938"
     CDS             complement(12982..13986)
                     /locus_tag="ACHU66_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HFR3389233.1"
                     /translation="MHHNTWKGIILQKQGLLKPQSVQQICQNLNGLQAQFQPYVHIGF
                     RNRMTNEAFQNQDWQASLTRQWSLRRTVHAYLKSEIPLYIHEGRMASVDYLETVSWDG
                     ALPQVKKKFYQVILEALETGPMTREELKILCRAENIGVEEEKQVFNAWGGLLRYMVER
                     GEIYQEYGVKRFHRLRDFQPLPKKEAELEIARRYFTGFGPVSLADARYYFKENKSTVL
                     KWMEQLDLKTVEVAGEERFYLGELEEAELPDCLFVAGFDQLLLGYEKKANPFFNPKYI
                     RNIYTLTGIVKPVVFYKGRFVATWKRDKGTILLDIFEDITQQEEKELEHYRQMFEKIL
                     "
     gene            complement(14051..14887)
                     /locus_tag="ACHU66_000939"
     CDS             complement(14051..14887)
                     /locus_tag="ACHU66_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR3389234.1"
                     /translation="MIKEFNATVRYLESVLESEIDEREFLRISGYSVPMFSRLFSILT
                     DMSLSEYVRLRKLTRAAMDIREGQAKIIDIAFKYGYESSDSFAFAFKQFHQISPSQVR
                     KGLPFKVLLPLQLSLTIQGGKEMDVRIEKKAGFTVAGLLRTAISNEVCPQVWEDLFKN
                     HTYEELAALGSGQSFGVCTLAQADETINYMAAYDVTDSEKATKLGLELLDVHEADYAI
                     FTLIGPVPQSIHAGWRYALETFFPEHGYRHSGAPDFEYYFEGDMSSPDYQMELWIPIV
                     KA"
     gene            complement(14964..16298)
                     /gene="trmFO"
                     /locus_tag="ACHU66_000940"
     CDS             complement(14964..16298)
                     /gene="trmFO"
                     /locus_tag="ACHU66_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HFR3389235.1"
                     /translation="MSQTHINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKPTPQHK
                     TDKFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRAGEAHRVPAGGAMAMDRENF
                     SQAVTDEIHNHPLIEVIRGEITDIPDDAITVIATGPLTSDALAEKIHALNGGDGFYFY
                     DAAAPIVDSSTINMDLVYLKSRYDKGEAAYLNAPMNKEQFTAFYEALISAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPEDYKGPRDGEYKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     FATKKNPNLFFAGQMTGVEGYVESAASGLVAGINAVRRFRGEEPVIFPQTTAIGALPF
                     YITHTESKHFQPMNVNFGIIKELDGPRIRDKKERYEAIAERSLKDLEEFLTV"
     gene            complement(16494..17243)
                     /locus_tag="ACHU66_000941"
     CDS             complement(16494..17243)
                     /locus_tag="ACHU66_000941"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFR3389236.1"
                     /translation="MKSYKNYIFDFYGTLVDIRTDENKLEVWNQLTQIYNAFGCSYRP
                     RQLKNAYHRFVEEAERFLAETVSYQYVEIDLETLFIRLLTDAPNENQSTNKPTDLETF
                     GQVVATIFRVLSREKLEAYENTLTSLQTLKDREARLFILSNAQRIFTQAEIEQTGCAD
                     LMEKIYISSDFKMKKPEPAFLQLVLEENNLDVKETVMVGNDLTSDIAIAHELGMDSIL
                     LNTFPYSQEEIDSYRRLGWNFEVVGDIAELL"
     gene            complement(17430..18638)
                     /locus_tag="ACHU66_000942"
     CDS             complement(17430..18638)
                     /locus_tag="ACHU66_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR3389237.1"
                     /translation="MTKNYKGVNRDKKGRIYIQTEFKANPITGKRQRYKSYLNRWGKP
                     FDTEKEAYNELCRVRAEYHDKFEYADYDISYKAFMNSVFLPYYKQTVQGLTYRTAMVH
                     FELFIEEFGDTKLRNITPRMCEQFRVKIIANYSPNYAKQLWCRFKQSLGYAERLEYIK
                     SFPCKSLDNPKGKRPETKFWTYEEFLKVINQFDLTDYEDHLRFVTVWLYYMTGVRVSE
                     GFSLIWSDFNAKKKTLHVQSTLEAIGKGQYLRKLQTKTEAGKRYISLDDETVRILSEW
                     RKIQVANSKDNYILSRFGEPMVKSTLSRMLKRYALKAGVPVITGKGLRHSHDSFMINV
                     LHLDVVALSHRSGRIDKATTLNTYSHYYQVDNTIGGQIQEVLEQSGVAKTKSDKTVGY
                     PTIHPTTIEG"
     gene            complement(18631..18879)
                     /locus_tag="ACHU66_000943"
     CDS             complement(18631..18879)
                     /locus_tag="ACHU66_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFR3389238.1"
                     /translation="MKTITHKELMKLGFAKTTARKIIRQAKNIAIQRFEVADKNSVNA
                     VKLSKSPFDNRRLDLAPLSIVEELLGFSLVEYEEKNHD"
     gene            complement(19215..19631)
                     /locus_tag="ACHU66_000944"
     CDS             complement(19215..19631)
                     /locus_tag="ACHU66_000944"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFR3389239.1"
                     /translation="MEYYLIVRGRKIFVDKKVYSGYWSLVNHQNYLRRREILFSVLPF
                     SSFEKENFRLEDVLPDLTVDIEKIVETKIMLELLSESLSRLNKSEMLLIESIYFQEKT
                     LRQVAEELKTSPCRISRLRDKVLNKLKNMLENSHKM"
BASE COUNT         5675 a         4329 c         3788 g         5939 t
ORIGIN      
        1 aatttatttt gtgtaaactt ttattataaa aatagaaagg aatactatat gattatttgg
       61 ttaaatggcc cattcggtat cggaaagact actttagcaa atatattaca caaacgaata
      121 gaaaactcat atctctatga tcccgagtta ctaggtgatt tcttacaaca tcaattacct
      181 aagactgtat gtcctgaaga ttttcaagat tattccgttt ggcgacggag tacttataaa
      241 atagtatttg atttagcgac taaaactgat aagattatta ttattcccat gacaatctat
      301 aagaaagaat attatcagga gattactcag caattgatac aggataagat tcctttggaa
      361 cattatattt tgttagctga caaaacaacc attttggaac gtttagataa tcgagtcaat
      421 gaagacaaca tctgggccaa gagacatctt gatgtttgtt taaaagcttt tgaaagtcat
      481 attccggggc aaaggttaaa cacagacagt ttgaagccag aagaggttgc ggaggaaatt
      541 ctaatgctaa gtgagtttac caagaaataa tgtagacttt tgggattttg ggtgtgaaaa
      601 aaaatttcca gttatttatg ttttttggtt atccctctga tatccccaca aacatctttc
      661 cgaaaaacta taattttctt gaaacttatg taagattatg ctatactact catatagcta
      721 aaatcaggag aaaatgatga gacgcgagtt attactagaa aaaattgatc aactaaaaga
      781 aattatgcct tggtttgttc tggaatatta tcagtcaaaa ttggctgtgc cttacagttt
      841 tactacctta tatgaatact tgaaagaata ccgtcgattc tttgagtggt tacaggattc
      901 ggatttggta tctgtggaac ggattgctga cattcctttg gatattctgg aacatttgac
      961 aaaaaaagat atggaagcct ttattcttta tctgcgggag cgtcccttgc tgaacgccaa
     1021 taccacgcag aatggtgtgt cgcagaccac cattaaccgt accctgtcgg ctctttctag
     1081 tctcttcaag tatttaaccg aagaagtgga aaatgagcag ggcgagccct acttctaccg
     1141 caatgtcatg aaaaaggtat ctaccaagaa gaagaaggaa accttggcag cgcgggctga
     1201 gaacatcaag caaaagctct ttttgggcga tgaaaccatg gagttcttgg actatgtgga
     1261 caaggaatac caagtcaatc tctctaaacg tgccctctcc tctttccaga aaaataagga
     1321 gcgagatttg gcgattctgg cacttctctt ggcttctggc gtccgtctgt cagaagcggt
     1381 gaatttggat cttcgagatg taaatctcaa tatgatgatt atcgaagtaa cccgtaaggg
     1441 tggtaagcgg gactcggtca atgtggctgg gtttgctaag ccctatctgg aagcctatat
     1501 gagcatccgt cagcaacgct acaaggctga aaaaacggat acggccttct tcctgtccga
     1561 ataccgtggt ctacccaatc gtatcgatgc ttcttctatt gaaaaaatgg ttgccaagta
     1621 ctctgcggac ttcaagatac gcgtaacccc ccacaaactc cgtcatacct tggcaactcg
     1681 cctatacgac gccacaaagt cgcaagttct ggtcagtcat cagctgggcc acgccaatac
     1741 tcaggtcact gacctctaca cccatatagt caatgatgag caaaaaaatg cactggatca
     1801 attatagtgt tacgtaatat taattatgta aataaattaa ggagtaagcg aaaacttacc
     1861 cctttttctc ttaaatctaa atcaatgctt tcaatttttc ctgattgaca gctgggtact
     1921 ggtccaaaaa ggctgagaga gttcgtaaaa cggcaggtat gtgtccagtc tggtcattct
     1981 ccacctgcaa gaccaagagt ggctcgtgga gactcatacg gagcaagagc cagccgtcac
     2041 cataaagctc agtcaggtca aaccgcactc cttcttcgtt ttctgggtta aaagcaaagc
     2101 cttcgaggct ggtgtttctg aaatcagcaa taacctgttc accaagtgct cgatagcctg
     2161 ccgcttctag tttgaaccgt acttcttggg tttcaagtgg ttgcttgagc tgggcaatca
     2221 agtcgtccaa ggacttgcct tccgcttgca gttttggtaa gagcataagg atcttagccg
     2281 ctacataggt cccgtcatcg aggaagtaat tttccttaaa ggcagcatgt ccagatgttt
     2341 cgatagccaa ctggcaatca actccctctt gattggctaa aatagcacgg ttgatgacat
     2401 tacggtagcc tgaaatatag cgaatttgtt tgcctcccag gctttctata aagaccttaa
     2461 ggtgttcagt cgttggggaa ttggtcacaa tgctggttcc tggatgttca gctaggacaa
     2521 tctggctgag aacggcaatc aggttgttac gattgagaat ttctcctgac ttggtaacca
     2581 gggcggcacg gtcaacgtcg gtatcaaaga taatgcctag gtctgcacct tgcttcaaga
     2641 cagcctgacg aatgctttcc atagcttcct tgttatctgg gtttggaaca tggtttggga
     2701 aggtgccgtc tgggtctagg aattgtgaac cagtggtatc tgctcccaat tctgccaaaa
     2761 ctttctcagc aaaaaatccg cctgcaccgt ttccagcatc gacgatgata ttaagacctg
     2821 tcagtggttt ttcttgtcca ccacaggcgg tacgaatttt gccaactaag tcttgggcat
     2881 aaggcgtaat caaatctgct ttggtcacac ttccaaggtc agaagccggc aagtcttcac
     2941 tactggagag gatgaagtca atatcttcat gttctgctcc accattttcc gaaaaaatct
     3001 taatgccgtt gaagtaatgc ggcagatggc tggcagtgat catgacacca gcatggcact
     3061 tgaactgagg atactgggtg ctcatgaaga gagctggcgt ggtagccata ccaaagtcaa
     3121 tcagctgaac gcccagacga acggcttctt ctgtaaaggc tgcgaccaaa tctggaccgc
     3181 tgaggcggct gtctcgaccg ataccgatgg tcaattggcc tttttgatag gtctgcgcca
     3241 gttccggctt ctgagttagc caatgaataa gtccgcgaac tacttcctgg gtggcttgcg
     3301 gagtgagatt gacagcatat tcgtctgtag cgatagcaat tccacgaata tctgatccat
     3361 tttgcaagac gtgatgatgg gacatagtaa cctcctaatt caaactaaat ttagtatatc
     3421 atattttcaa agcggtttca aaatgaaaaa agcacagctt ctactgtact tttgtgatgg
     3481 ttacgctggt cccaacttcc agttgacttt ccacggaaat agctagttcc aactgattca
     3541 ggacccgctt ggtcagatac aatccaaatc ctgtcgcttt ttggtgttct cggccattaa
     3601 aacctgtaaa tccctcgtca aacaggcgtg gaatatcttc tgccagaatc ccaattcccg
     3661 tatcggaaat ggaaattccc tcattcagtt caatggctac ctttctacca gtcttattgt
     3721 acttgagggc gttgtcgatt atctgtgaca aggcaaagct gagccatttt ctgtcggact
     3781 tcagtagcca atttccatcg atggtcactg aaatgtcttt ttgtaagaac tgattgcggt
     3841 attttttgac caaatccacc accacttctc ttacttccac ctgctcaaat cgaaaatcgc
     3901 tggcatggtt ggtcagcttg aggtagttga ggagatttgc catataattg tccaagcgta
     3961 agagctggtg attgacatcc tgctggttga gattgtcagt ctggctcatc aaagacaggg
     4021 cagccagagg cactttcatc tggtgggacc acattttgac catggcttgc aggtcctctt
     4081 ctcgtgactt atagaccaaa agctgctctc gtgtcgcctc cttctccgct tcaatcaagt
     4141 tgagataggc aaggtccaca ggcttattga gcaagggcaa ggcttcctca tgaacatagt
     4201 cttccaaggc tctcatgcgg ttgcgaaact tccagaaaag ccctacagtc agtaggatta
     4261 aaagggttag ggacaatagc agagcgtttt gcaggaattc ggtgggcagt tgatagagaa
     4321 agaaaagcaa gataaaacta gtagccagaa caatgtagga gaagtaaaaa ctccggtatt
     4381 cacggataaa ttttgctatc atttgaccaa gtaccccacg ccacgaaccg tatgaattcg
     4441 ttcaaacccg acttcgctca ccttcttacg cagtctggtc atgttgacat tgagcgtgtt
     4501 ctggtcaata aattcttcat tttcccagag tttttccagg agttcttcct tgctgacaat
     4561 ctgcccttgt ctttctagta aggcggacaa aatcttggtt tccgtaggag acaattgaac
     4621 ctgttccaat tcgtttgaaa atcgcccgtc taaactaagt tggaactggt caattgacaa
     4681 atcagttgat ttgctgaatt gattaacccg acgcaagaag gcgccgattt tggcatccaa
     4741 aatccccaga gaaaacggct tagacacaaa atcatcgcca cccatgttca tggccatgac
     4801 cgcattcatc tcatcatcgc tagaggagat aaagataatg ggcatggtca tggttttacg
     4861 gatttctgtc gtccagtaaa aaccgttgta ataaggcagg ctaatgtcca tcagcaccaa
     4921 gtctggtttg atttcagcga cttcctgact gacagctcgg aaattctgca cactttccac
     4981 ctgatacgat tttcccaaat gctgcttcaa gagctggaca atcatctcat catcttcaac
     5041 aatataaatc ttctcttttt tcatcttctt cattctgcaa aagccaggac acaggtcttg
     5101 gcttatatac ttccatttct catttttatg cttgtcacaa tcaatttatc atcttcaata
     5161 ttaacgataa ccctgtaatt gccgatacga tagcgccatt ggccagaacg attagcagtc
     5221 aaggcttttc cgtattgtct agggttgtcg caaccgtcaa tatgcttgta gagatagcgc
     5281 aaaatcaaac ttgcagcggt tttatccatc tttcgaattt gcttttgggc cttagcagac
     5341 aaaataacct tataactcaa tgcccaactc ctcagccatt tgttctaatg tcatcggctc
     5401 aattctattc gccaagtctg cttggtattc tgcccaagca acgtctgcca cacgcgcatc
     5461 gtactcgtct tcaagggctt caagggtttt agtccgaatg agttctgaca aactgacacc
     5521 ttcaaacttt gccatagcct gcataaagag cttgtcctgc tcagaaactt tcaatgtaat
     5581 agctgccatt ttcttacctc tttgagtatt cctttggtat taccattgta acaccatttc
     5641 cctgaaaaag caagaaatct cgcacaaggc gagacttctg ctatcgttca ataatacgat
     5701 aataggttcg gctggtccac ttgtaaatga caaagtagat gaggacaaca gcaagcaaag
     5761 taataccact aaccgtataa atcatgctgg aagaaactac accgaacacc atcaacattt
     5821 gcttgagcat gactagtgcg acgataaagt gcagggtcgc catagccagt ggcataaaga
     5881 agaccaagag tgtttgagag ttgatggttt tcttaacagc ctgctggctc atacccactt
     5941 cctgcaaaat cttgtaggat ttcttgtcct catgtccttc cgagtactgt ttgtagtaga
     6001 taatcaaggc tgcaccaagt aggaaactaa tgcccagcaa gaaacctgta aagaggaaac
     6061 tgcctgtaaa gcccatcagt aaattgttaa aatctgattt ttgcatagca tatcctagga
     6121 attctccgtt tgcatcgttg atattataag agtcgccagt ttgttgacca atcgaagcaa
     6181 cctcttcatc agtcaagtcg gtaaagacac ggtagtccag actgactgta tacccttgcg
     6241 gattattgga ctcgtaatac tcacgaacag catccaacac agtgtcatca ctgactacca
     6301 taacggcggc atttagcgta ttggtgatgt ctgggaaaat agcctggtca agattggcta
     6361 cattctcaaa ggtttgatcg cccaaaacaa gcttcttaag cgacggcatt tcactcggac
     6421 gcataaaggc aacttgattg gcagccagct ctggcaggtc attccccaat ttcctgaaat
     6481 catcttgggt gatgaagtag gtaaaggcca ttttgctaaa gtctggattg gcaatggttt
     6541 ctggattgat gacaatatcg ctcccaccat catagaccaa acctgcttgg taggtcagat
     6601 aggatagatt gtcattggct ttttctggaa aatggctgag caccgattgc tgataggcag
     6661 ctacaccttg acttctatac gaaactttat aggtaataga tgtttccttg ggatagagtc
     6721 cacttgtcat atttgccata cctgtataga gggatgtcgt tgtcgcaatg gtcacaaagg
     6781 ccatgaccgc aagtagcgta atattagcca aacccgttgc gtgctgcttc atccggaaaa
     6841 tcatctgcga agtcgtaatg aaatgctccg gcgtatagaa ataccgcttg tttttccgac
     6901 gagccttcaa ataccaagtc ataaaactga tgtagaacag ataggtcccc gcaatgacaa
     6961 atagcacagc gataaagaac cgatagataa cagctagacc ttctgctttc gatgaaagcg
     7021 ataggtagta gcccatccca agactaagga caccaagagc agctagaaga acatttcctt
     7081 tcggctctct ctctcctttt tcactagctc ggaacaaaat cagcggactg gtcttaccaa
     7141 tggttcgaat ggccagcaat tccaagagga aaaagaaacc agcaaagaga aaggcagtca
     7201 tgacaaaggc cagaggctca attccaaaat gtaaatcact ataatgtgtt atattgacaa
     7261 aaatcaagta gagaacattt gcaaacacac cagctaggac agaaccaaga accacaacca
     7321 gtaagaatat catcatcaat tcgatgctgg ctattaaggc tacttttttc ttgttcatcc
     7381 ccagcatgtt atagagacca aattccctcg cccgttgttt catcaggaaa ttaaaactat
     7441 aaatctccat aatgactgag aaaatggtca gaacaacgac acccagcccg atagacacgg
     7501 cacctgttcc catggttttc atgactggac taagcatgag caagaacata gaacaaatca
     7561 aggtaaagag gaccagacta gctaaaacga agggagcaaa aacacctatg gattttcgga
     7621 tattttgtcc agcaagtttg aggtaaaaca tctactcacc ccctaaaagt gcagacatat
     7681 tgagcgaaat ctctttggca aagtcttgat tgctcttacc tgccttgtac aattggtgga
     7741 aaatccgacc atccttgatg aagaggacgc gtttggcatg gctggctgcg ttggctgagt
     7801 gagttaccat gagaattgtt tgtccatcta ggttgatgtc ttcaaacaaa ttcagcaagt
     7861 cttccgaatt gcggtaatct agggcagccg tcggttcatc cgctagaaca atctgcggct
     7921 gggtaatcaa actacgggca atggccacgc gctgcttctg tccaccagac agctcaaaag
     7981 gacgtttttt cagcaaatct tgaatccgca gttttggcgc caaaactttc aaccgtttct
     8041 ccatttcctc atagctctca cgcgccaata ccagaggtaa aaagatattg tcttggatag
     8101 acagggtgtc caagaggtta aaatcttgga agacaaagcc tagattacgc aaacggaaat
     8161 ctgccagctt gctttccttg attttggtga tgtcttgatt atttaagata acagagcccc
     8221 tcgtcggctt gtccagggtt gctagaatgt tgagcaaagt cgtcttacct gaaccacttt
     8281 cacccatgat ggcgataaac tcaccctctt ctaccttaaa atccacatct tgcaaagcac
     8341 gagtttcctc ttttgaaaag cgcgtgcggt aaactttttc taaatgattg atttctaata
     8401 acatagttac tcctaattcc ttattcactg tctaaatcaa cttggtcaag aactgcttga
     8461 tagtcgattc ggtctgattc agcaatatta tcttctaaat ccaccttata ataaccatcc
     8521 tggacaccga tggcccattc cttgctagtg gttatataga gattattcaa ggatactgtc
     8581 tttttcactg ccccctcttc tttgacaaca ggccattcca taaacttgac ttctacttct
     8641 tgaatattat tctcggcaac ttcctgtttg ctaacggcat agtcttttcc cttgtaatgg
     8701 aaagtttgat agccctcatc aaagctcagg cctgctgaag agaaagaaca agcggttagg
     8761 attatgctgc ccaaagccag caagccaaat actttctttt tcatagtttc ctccctctag
     8821 tatgccataa atcgcattgt ttgcaaatca agtgccagct cctctaaaaa ttctgcctgt
     8881 tccaagagtt cagtcttttc tttttctgtg aatgcctttt cttccattct ctttacttgt
     8941 aaaaatttaa atgacatatg atttccttct ttctgtttct tatgagtcta ttatagaaaa
     9001 tatgtcgctc atgttctctt acagttctga ttaaaaaact tacaagagtg taagtttaga
     9061 gaaaataacc aatcgtggcc agtatccaca aatgaagcgg atagaagatg taaaagaaat
     9121 agcgtgaaaa tactgtgcgg ggccctgctt gtccattata aagactaaga attggaagga
     9181 ctaaaataaa tagccagtct gcattgtaga gcatcatctg aatggtcatc tcaagccttt
     9241 cgtagggctg gtaggacatg gctaataaaa ctagagccag aaaaccatag gcaatggttc
     9301 gcttagtagc agactcacga cccaaatagg tgatgaggat gaatggcaag accaccgtgc
     9361 ccccttctgt gaacaaggca cctatgattg tcaataaccc tgccagcagg acctgaatga
     9421 tttgcttcgc tctcgtgtgc tcactaaaat tttgaagaca aatcagcaga gttaggccga
     9481 ctgctaaggt caaaaagata ttattgctaa tgtaaatccc tttagactgg tagagactgt
     9541 ttagcaaagt atttccagtg gccattagtc ctgctgcacc atacagtcgt aacagatagg
     9601 ctttgacatt tctcgtatga agaaaccctt ccaccaacat gtaggcaaag aaaaccgcga
     9661 cgcaccgtgt tgctaaatga aacactaaag cgagttgatc agggataaaa tcaggtatga
     9721 ttttcagatg atccaaaacc atcaagcctg ccatagcgag tttcagttga taagcattat
     9781 atgatttcac cagtcaaacc tcctagtaca tccagttgcg ttggaacagt tcgtgttcta
     9841 gctcgaatag aaattcagct tgtttgtctt gttcttctag tcgtttgctt tcttgtgatt
     9901 taccaaaaat ttgttttgct tgtagaaatt tgaatgacat ctgatttcct tctttctatt
     9961 tcttatgagt ctattataga aaatatgtcg ctcatgttct cttacaattc tgaactaaaa
    10021 acttacaggt ttgtaagaaa agaaaaaaga caccatttta ggcatctttc aattcattct
    10081 attttgttaa accaatgtag gcaatcattt catccaactc tttcttgctt tccgttacaa
    10141 gtagataggc atcatttccc tgaagttccg caaaggcatc tggcatgcgt ttgacggttt
    10201 taatcttacc agcgaatttc tgatgaatgt catctgctga gtgaacatat tcggtcgtat
    10261 cacggcgagt tgcgaccaag cagtattgtg gttcaaatct acgttcctca actgtaccac
    10321 ctgttgcgac attggcataa tcacggaaaa gatcaatgga atgggcgtag ttataggctt
    10381 caacagtaaa gccacccgcc atgcggttgt tgtattcaat ggcaatgtag tcgtccccat
    10441 ccttgaaaaa ttcaatatgg aaaaaacgct ctctcatacc aaaggccttg ataatcgcgc
    10501 gaccatattt tactaattga ggatccaact ccttctcaat gaagtaggca ttgtcttttc
    10561 tagacaacat cagttccagc ggagtatgaa cataagtcaa acctgtttca aagacaacat
    10621 tgccttcgtg gtccaacaaa ccatcatagg ttgtgataat gcttgagttg acaaattttt
    10681 cgaggaaata aatagttgct ccatcccatg tctccacaaa ccgatcaaaa tcttctttat
    10741 tttcgatttt aaaggtaccg gatgccccaa caccgttgtc tggtttagca atcattggga
    10801 aaccaacatt cttcaaagct ctgccaaaat cacgttccga tttaacaacc acacctggaa
    10861 cgactggaac acctgctttt ttgaaatatt ttttcatttc agacttaaat ttagtctttt
    10921 tcaggtgttt gggtttggct ccaaaaatgt tgaattgttc acggagggct gcatcgtttt
    10981 caagccaata ttcattgtgg gattctacac ggtcaattgg accatgcttg taaataagaa
    11041 aggccgctgc acgcttgact tcatcgatat tttccaaatc ttccacacga aagtattctg
    11101 taagagcgtt acgaagttct tcaggcaatt gatcgtaagg ttcttgccca atacctagaa
    11161 ccgtaacgcc ttctttttta gccaattcaa tggtgaacgg ttgaaaattt tctggataaa
    11221 aaggtgaaat aacgagataa ttcataggta gtcctttcta atcttaaagt ccgatagaat
    11281 gaaggaagaa tggcatttgc tttctccacc acacccagtc gtgggccaca tctgttcccc
    11341 aagtatcaaa ccaagctggg atattcttct cttcaaaggc acgttttagg ttgtagaatg
    11401 aatccagacc gtcttgctcc caatcgccca aacctgtgca gacgataata tctgcttgac
    11461 ggtatttgtc aataaaccaa ccatcattct ggttccagat gtaatcagac ggtgagtttt
    11521 gatagaccgc atcatcatga ccataatcat ggttgttgtt gaagaatcga gcatcataga
    11581 cacctgaaag tgcaataact ttcgtgaaga catctggatg ctggaggaag aaattgagcg
    11641 cgtggtaggc ccccattgag caaccagttg tcatcatacc atcgaaccag ccagttttat
    11701 gtttgataaa gggaatcgct tcttcaatca cataccgttc ataggcgcgg tgagcctctg
    11761 ccttatcatg aatggatttc cagtctgcca accagctttc actatcgtag ctagttagag
    11821 taaaaaattg gacctgacca gcttcaatag atccacgaca agcttcaatc ataccaaaat
    11881 cagcatattc attgtaagaa ccacctgatg aagcaaatac gacaacagga atccctgcgt
    11941 gaccataacg atttagattc atttctcggc caagatttcc agaccaatga cttaaaaatt
    12001 caacgtgcat agtaaagcct cctatttgta tatttttgtt ttatgaccaa tgttcgctta
    12061 ggaatcgtag gcaagctggc aaatagagcg cccaagcctc ttcattatgg accgcacccg
    12121 aaacgacttc caaggcgata ttgtccaaat ccactccgcc agcgatcaat tgacgatagt
    12181 aagtcaatga agaattgata taggcctgct tgatatttcc tgccatcaat gttttatctg
    12241 tatcatctgc ttcttctgtc ccaacgtaga tgtagactcg ttgctctgag tctaattgtt
    12301 gacgttcaat ataacggtcg aaagcatctt gatgaagcca attggccgat gaaaagacac
    12361 ccagacagcc aatctggttc tgataagcca agcccataaa ttgtgtgata ttgcctccca
    12421 aggatgagcc aatcattgca gtgtgaactt tatcagattt tgtccggtat tctttatcga
    12481 taaagggttt gacgacatcc atcacaaatt cagcatacaa ggttccctta ccaccgaact
    12541 gcaccccagg aatccccatc tcactaaact tccaagcagc gtattcatgc atgcgatcag
    12601 caccatcgtt atcaatagct actacgatca tttttgcaat gtctggatta cgcttaatag
    12661 ttgggatgac tttccatgaa tgacctgaaa aagcttcctt actgtagagt acattttgcc
    12721 catcgtggaa ataaacaacg ggataggtct cttctgtatt ttcagcatat cctttcggca
    12781 ataaaacacg gactcgacgt tttcttcctg taaatggtac atctaattca tgagttctca
    12841 tgtccaaata aaagtaagac ttattcatat ctatctcttc cttatgtatt ttaagtatta
    12901 tatcatagtt tttctggaat attcagaaaa tttagttatt tatttgcaat tttcggattt
    12961 tccgaaaata gttagttaat gtcacagaat tttctcaaac atttggcggt aatgctccaa
    13021 ttctttttct tcttgttgtg tgatatcttc aaaaatatct agcaaaatag tacctttatc
    13081 tcgtttccag gttgccacaa atcgcccttt ataaaataca acaggtttga caatacccgt
    13141 caaagtatag atattacgga tgtatttagg attaaaaaag ggattagctt tcttttcata
    13201 gcctagcaaa agttggtcaa atcctgcaac gaagaggcag tcagggagtt ctgcttcttc
    13261 taattctccc agataaaagc gttcttctcc tgctacctca acggttttta aatctaattg
    13321 ctccatccat tttaggaccg tagatttatt ttccttgaaa taataacgag catccgctaa
    13381 actgactggc ccaaaacctg taaaataccg tcgagcgatt tccagctctg cttctttctt
    13441 tggtagtggt tgaaagtcac gtaaccgatg aaaccttttt accccatact cttggtaaat
    13501 ttctcctcgt tcaaccatat aacgaaggag accaccccat gcattgaaaa cttgtttctc
    13561 ttcttctact ccaatgttct cagctcggca aagaattttc aactcttcac gagtcatagg
    13621 gccagtttct aaggcttcca aaataacttg ataaaatttt ttcttaacct gtggtagagc
    13681 accatcccaa cttactgttt caagataatc aacgcttgcc attctccctt catgaatata
    13741 gagaggaatt tccgatttga ggtaggcatg aactgtccgt cgaagtgacc attggcgtgt
    13801 cagtgaggct tgccagtcct gattttgaaa tgcttcattg gtcattcgat tacgaaaacc
    13861 gatatgtaca tagggctgaa attgtgcctg caaaccattg agattttggc agatttgctg
    13921 aacagactga ggtttcaaca gaccttgttt ctgcagaata attcccttcc aagtattgtg
    13981 gtgcatcgta tttctcctcc gctttctaat cccagtataa cacaaaagac acccgaaggt
    14041 gtcctgtaca ttatgctttc acaatgggaa tccagagttc catttggtag tctggggaac
    14101 tcatgtctcc ttcaaaatag tactcaaaat ctggtgcacc tgaatggcga tagccgtgtt
    14161 caggaaaaaa agtttctagc gcataacgcc atcctgcatg aatagattga ggaacagggc
    14221 ctattagggt aaaaatagca tagtcagctt cgtgaacatc aagaagttct aagcctaact
    14281 tagtcgcttt ttcactatct gtgacatcat aagcagccat ataattaatc gtctcgtccg
    14341 cttgagctaa tgtacaaact ccaaaggact gcccacttcc tagagcagct aactcttcat
    14401 aagtatggtt cttgaacaag tcttcccata cctgcggaca cacttcgtta ctgattgctg
    14461 tgcgaagaag tccagcaact gtaaaaccag ccttcttttc aatacgaaca tccatttctt
    14521 tacctccttg gatggttaat gatagttgca agggtaagag gaccttaaaa ggcaacccct
    14581 tacgcacttg actaggagaa atctgatgaa attgtttgaa ggcaaaggca aaagaatccg
    14641 aagattcata cccatattta aaggctatat ctataatctt tgcctgtcct tcacgaatgt
    14701 ccatagcagc tcgcgtcaac tttcgtaacc gaacgtattc tgacaaggac atatctgtca
    14761 gaatcgaaaa gagacgacta aacatgggaa cggaatatcc tgatatgcgc aaaaattccc
    14821 gttcatcgat ttcgctttct aaaacggact ctaggtaacg tacggttgca ttgaactctt
    14881 ttatcatcaa cttccccttt ccctatcagt ttaccgtcta ccatccaagt tttcccgata
    14941 cttttcgtcg gaaaactatc ggcttaaacc gtcaaaaact cttccaagtc cttgagggaa
    15001 cgctctgcta ttgcttcata acgctccttc ttatcgcgga tacgtggtcc atccagctcc
    15061 ttgatgatgc caaagttgac attcataggc tggaagtgct tgctttctgt gtgggtgatg
    15121 tagaatggca gagcaccgat ggctgtggtc tgtgggaaga tgaccggctc ttcgcctcgg
    15181 aagcgacgaa cagcattgat accggccact aagccagaag cggcagactc aacatagcct
    15241 tctactcctg tcatttgacc tgcaaagaaa agatttggat ttttcttggt cgcaaaggtc
    15301 tgttccaata ggtttggcga atccatgtag gaattgcggt gcatgacacc ataacggaca
    15361 aattcagcat tttcaaggcc tggaatcatc tggaagaccc gcttctgctc gccccactta
    15421 aggtgagtct ggaagccaac aatattgtaa aggctacctg ctgcgttgtc ctgacggagt
    15481 tggacaacag catagggtgt cttgtattca ccgtcacgag gtcccttgta gtcttctggg
    15541 tattccagac cgactggctt catcggacca tacaacatgg ttttgatccc acgtttggcc
    15601 atgacttcaa tgggcataca gccttcaaaa tatttttctt tttcaaagga attgagcggt
    15661 gcttcctcgg ctgaaatcaa ggcctcatag aaagcggtaa actgctcctt gttcatcgga
    15721 gcattgagat aggcagcctc tcccttatca taacgggact tgagataaac caagtccatg
    15781 ttaatggtcg aactatcgac aatcggtgct gccgcatcgt agaagtaaaa accgtcgcca
    15841 ccattgagtg cgtgaatctt ctccgccaag gcgtcagatg tcaaaggccc cgtcgcgata
    15901 actgtaatag catcgtcagg aatgtccgta atctcaccgc gaatgacctc aatcagtggg
    15961 tgattgtgaa tctcatctgt gaccgcttgg gagaaattct cacggtccat ggccatagca
    16021 ccgccagcag gcacacggtg agcctcacca gctcgcatga taatggaatc caagcgacgc
    16081 atttcttcct tgagaagacc gacagcgttg gtcaaactat caccacggaa tgaattggaa
    16141 caaaccaatt ccgcaaactt gtccgttttg tgctgaggag tcggcttgac accacgcatc
    16201 tcgtaaagtt tgacaggaat gccacgtttg gcaatctgat aagcagcttc tgagccagcc
    16261 aagccagctc cgataacgtt aatgtgggtt tgagacatga aactaatacc tctagtcaga
    16321 atatctgact actgtttggg aataccccaa aacagaacct ttctaaattt tatacctatc
    16381 tagtatacca gaaaatggct agaaaagaca aaactacctt cattgaaata aaagtagttt
    16441 caccttttta cataatatat tttatgtata ttctttactg ttctatcgct tatttacaat
    16501 agctccgcta tatctccaac aacctcaaaa ttccaaccaa gtctcctata agagtcgatt
    16561 tcttcctgac tatacgggaa agtattcagc aaaatggaat ccattcccaa ttcatgagca
    16621 atagctatat cggatgtcaa atcattacca accataaccg tttccttaac atcaagatta
    16681 ttttcctcta aaaccagttg aagaaatgct ggttcaggct tcttcatctt aaagtcagag
    16741 gagatataaa ttttttccat caaatcagca caacctgtct gttcaatttc tgcctgagta
    16801 aaaattcgtt gagcattgga gagaataaat aatcgcgctt ctctatcctt taatgtctgt
    16861 agactagtta gggtattttc ataagcctct aacttttcac gtgaaagcac gcggaaaatc
    16921 gtggctacta cttgcccaaa agtctctaaa tcagtcggct tattggtact ttggttttca
    16981 tttggtgcat ccgttaatag gcgaatgaag agtgtttcta aatcaatttc gacatactga
    17041 taactcactg tttccgcgag aaatctctct gcctcttcta caaaacgatg ataggcattt
    17101 ttcaactggc gcggtcgata ggaacaacca aaagcattgt aaatctgtgt tagctgattc
    17161 cagacctcta gtttattctc atctgtacga atatcaacca atgtaccata aaaatcgaag
    17221 atatagtttt tgtaactttt catgtctttc tcctaatctt tagacatcat tttttatatt
    17281 ataccccaca acgaaaacgt ttgcaacaat agaacgaaaa aacctaccac attaagtgat
    17341 aggtttttat tttgattgga aactctatta tttaagagta acagttgcat tcattcaatt
    17401 tttccctgta ttatcaatac ttttcaaaac taaccttcta tcgtggtggg gtgtatggtg
    17461 gggtatccta cagttttatc tgattttgtt tttgccacgc cactttgttc taacacttcc
    17521 tgaatttgtc caccaattgt gttgtctact tgataataat gtgaataggt gtttaatgta
    17581 gttgctttat caattcttcc tgatcgatgt gataatgcta caacatctaa atgtaagaca
    17641 tttatcatga acgaatcatg tgagtgtctt aatccttttc ctgtaatcac tggaacacct
    17701 gctttaaggg catatctttt taacattctg gataaggtac tttttaccat tggttcacca
    17761 aaacgtgaaa gaatataatt atcctttgaa ttcgcaactt gaatctttct ccattcagac
    17821 aatatccgaa cagtttcatc atccaatgaa atataacgct tccctgcctc tgttttagtc
    17881 tgtaattttc ttagatattg tccttttcca atcgcttcta gtgttgattg gacatgaagt
    17941 gtttttttct tagcattgaa atcggaccaa atcaatgaga atccttcact tactcgaact
    18001 ccggtcatat agtataacca aactgttacg aatcttaagt ggtcttcata atcagttagg
    18061 tcaaactggt ttatgacttt taaaaattct tcgtatgtcc agaattttgt ttctggtctt
    18121 ttcccttttg ggttatccaa tgatttacat ggaaacgatt ttatatactc taaccgctca
    18181 gcataaccta atgattgttt aaacctacac cacagctgtt ttgcgtagtt aggtgaataa
    18241 ttggcaataa tcttaactcg aaactgctca cacattcttg gtgttatgtt tctcaatttg
    18301 gtatcaccaa attcctcaat aaataattca aaatgaacca ttgcagttcg ataagtcagc
    18361 ccttgaactg tttgtttata gtatggtaag aatacactgt tcatgaatgc cttataagaa
    18421 atatcataat ctgcatattc aaatttatca tgatactctg cacgaactct gcacaattca
    18481 ttatacgctt ctttctcagt atcaaacggt ttaccccacc tgtttaaata acttttatat
    18541 ctttgtcttt tgccagttat aggatttgct ttaaattctg tttgtatgta tattctacct
    18601 ttcttatctc tatttacacc tttataattt ttagtcatgg ttcttctcct cgtattccac
    18661 gagcgagaaa cctagtaatt cttcaacaat agataacggt gctaagtcta atcgcctatt
    18721 gtcgaatggg gatttactta attttaccgc atttacacta tttttgtctg ctacttcaaa
    18781 tctctgaatt gcaatatttt ttgcttgtcg tattattttt ctagctgtag tttttgcaaa
    18841 ccccaatttc attaattctt tgtgagttat tgtcttcata gaatacctct atctatccga
    18901 aacgattcga atgcatgtta ctccgaacta agtttcacga cattggtggg ttgatttgga
    18961 aatcaacata ggaatctcac ctgctccatt tttcgactag agcctgaacg ttcagaagta
    19021 tcattaacat tatttgtgtc atggcaaatt ggtttccagt caatttttga actagatagt
    19081 ttctcgctca tatcatcgcg tatcttattc agctgctcga caaataaata aagtcccacc
    19141 ttggtctatt caattttcaa aggacataaa gaaaactctt tcactatata aagtgaaaaa
    19201 gttctcggtc tgtttcacat tttatgcgaa ttttcaagca tgttttttaa cttattaagg
    19261 actttatctc ttaatctaga gattctgcat gggctagttt ttaactcttc agcaacttga
    19321 cgtagtgttt tttcctgaaa gtatatagac tcaattaaaa gcatttcact tttatttagt
    19381 ctcgaaagtg attctgatag tagctccaac ataatttttg tttctacaat tttctcaata
    19441 tctacagtca aatcaggtaa aacatcttct aatctaaagt tttctttttc aaacgatgaa
    19501 aacggaagta cggaaaataa tatttcacgt cttcgtaaat agttttgatg atttaccaga
    19561 ctccagtaac cactatatac ttttttatct acaaaaatct tccgtcctcg tacaattaaa
    19621 taatattcca tagtattcct ccaattgcta aaaacttatt aagtacaatt ggaggactcc
    19681 gtgtcctact catacttgaa actctcttta tttttttcct ccctacctag t
//
LOCUS       DAZQCJ010000029        19246 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000029 DAZQCJ010000000
VERSION     DAZQCJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19246
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.029"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            18..416
                     /gene="spx"
                     /locus_tag="ACHU66_000945"
     CDS             18..416
                     /gene="spx"
                     /locus_tag="ACHU66_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HFR3389240.1"
                     /translation="MIKIYTVSSCTSCKKAKNWLNAHQLSYNEHNLAKEAITKEEILN
                     ILTKTENGIASIVSSKNRYAKSLDFDIEELSVNEVIDLITSNPRILKSPILIDEKRLQ
                     VGYKEDDIRAFLPRAVRNVENAQARMRAAL"
     gene            516..782
                     /locus_tag="ACHU66_000946"
     CDS             516..782
                     /locus_tag="ACHU66_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFR3389241.1"
                     /translation="MGFTEETVRFRLDDTDKQEISKTLTSVYRSLEEKGYNPINQIIG
                     YVLSGDPAYIPRYNDARNQIRKHERDEIIEELVRYYLKGNGIDL"
     gene            782..1201
                     /gene="ruvX"
                     /locus_tag="ACHU66_000947"
     CDS             782..1201
                     /gene="ruvX"
                     /locus_tag="ACHU66_000947"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR3389242.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIPIDEEKGEFGLER
                     LTELVEQYKVDKFVVGLPKNMNNTSGPRVEASQAYGDLLTERYKLPVDYQDERLTTVA
                     AERMLIEQADISRGKRKKVIDKLAAQLILQNYLDRTF"
     gene            1213..1533
                     /locus_tag="ACHU66_000948"
     CDS             1213..1533
                     /locus_tag="ACHU66_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFR3389243.1"
                     /translation="MAHHHDHEHDHNHDERELITLVDDQGNETLFEILLTIDGQEEFG
                     KNYVLLIPASAEEDENGEVEIQAYSYIENENGTEGDLQPIPEDATAEWDMIEEVFNSF
                     MEEE"
     gene            1791..2369
                     /locus_tag="ACHU66_000949"
     CDS             1791..2369
                     /locus_tag="ACHU66_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HFR3389244.1"
                     /translation="MKDSFTERSKRLSEELERCLLADKNILVILDIMDRLNLSDCWLC
                     AGTIRNFIWNQYSFDEETDVDLVFFDENISYEETMEIESDLYQNYPAYRWELKNQVYM
                     HIHSPNTKPNQSSRDAIEKFPERCTAIGICRTERNKLEIFAPFGLDDIYAYQVRPTPH
                     FLADPNRMELYRQRILKKQWKKRWLSLTVKET"
     gene            2442..2708
                     /locus_tag="ACHU66_000950"
     CDS             2442..2708
                     /locus_tag="ACHU66_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR3389245.1"
                     /translation="MEFETVCLKVRNLGRLYCYFKVKFRNHIEAIIRKQESQKCKFDR
                     LFHEDIHSLIQSPSLGNDELLMLWGLLRDYRKNLINDRLENMKN"
     gene            3030..3338
                     /gene="rpsJ"
                     /locus_tag="ACHU66_000951"
     CDS             3030..3338
                     /gene="rpsJ"
                     /locus_tag="ACHU66_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR3389246.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVETATRTGAQVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIINPTQKTVDALMKLALPSGVNVEIKL
                     "
     gene            3551..4177
                     /gene="rplC"
                     /locus_tag="ACHU66_000952"
     CDS             3551..4177
                     /gene="rplC"
                     /locus_tag="ACHU66_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR3389247.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYAAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGQEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGNRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            4202..4825
                     /gene="rplD"
                     /locus_tag="ACHU66_000953"
     CDS             4202..4825
                     /gene="rplD"
                     /locus_tag="ACHU66_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR3389248.1"
                     /translation="MANVTLFDQTGKQAGEVVLNDAIFGIEPNQAVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYAYKLP
                     QKVRRLALKSVYSEKVAENKFVAVNSLEFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNIPGVKVATATTASVLDIANADKLLVTQAAISKIEEVLA"
     gene            4825..5124
                     /locus_tag="ACHU66_000954"
     CDS             4825..5124
                     /locus_tag="ACHU66_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR3389249.1"
                     /translation="MNLYDVIKKPVITESSMGQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKTKRVGRYVGRTNKVKKAIITLAADSKAIELFATADAE"
     gene            5142..5975
                     /gene="rplB"
                     /locus_tag="ACHU66_000955"
     CDS             5142..5975
                     /gene="rplB"
                     /locus_tag="ACHU66_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR3389250.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDGVEAIVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTVVHNIELKPGRGGELVRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATVGTVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNQ
                     K"
     gene            6077..6358
                     /gene="rpsS"
                     /locus_tag="ACHU66_000956"
     CDS             6077..6358
                     /gene="rpsS"
                     /locus_tag="ACHU66_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR3389251.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            6376..6720
                     /gene="rplV"
                     /locus_tag="ACHU66_000957"
     CDS             6376..6720
                     /gene="rplV"
                     /locus_tag="ACHU66_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR3389252.1"
                     /translation="MAEITSAKATARTVRVSPRKSRLVLDNIRGKSVADAIAILKFTP
                     NKAAGIIEGVLNSAIANAENNFGLEKANLVVSEAFANEGPTLKRFRPRAKGSASPINK
                     RTAHITVVVAEK"
     gene            6733..7386
                     /gene="rpsC"
                     /locus_tag="ACHU66_000958"
     CDS             6733..7386
                     /gene="rpsC"
                     /locus_tag="ACHU66_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR3389253.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRNFIKK
                     ELADASTSTIEIERAVNKVIVSIHTAKPGMVIGKAGSNVDALRAQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGESIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEALTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            7390..7803
                     /gene="rplP"
                     /locus_tag="ACHU66_000959"
     CDS             7390..7803
                     /gene="rplP"
                     /locus_tag="ACHU66_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR3389254.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKQVDFGQYGLQATTSSWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEVAGVSEEIAREAFRLAGHKLPVKVKFVKREAE"
     gene            7813..8019
                     /gene="rpmC"
                     /locus_tag="ACHU66_000960"
     CDS             7813..8019
                     /gene="rpmC"
                     /locus_tag="ACHU66_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR3389255.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFELRFQAAAGQL
                     EQTARLNEVKKQIARIKTVQSETK"
     gene            8043..8303
                     /gene="rpsQ"
                     /locus_tag="ACHU66_000961"
     CDS             8043..8303
                     /gene="rpsQ"
                     /locus_tag="ACHU66_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR3389256.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            8328..8696
                     /gene="rplN"
                     /locus_tag="ACHU66_000962"
     CDS             8328..8696
                     /gene="rplN"
                     /locus_tag="ACHU66_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR3389257.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDIIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRADGSYIKFDENAAVIIREDKNPRGTRIFGPV
                     ARELRDGGFMKIVSLAPEVL"
     gene            8835..9140
                     /gene="rplX"
                     /locus_tag="ACHU66_000963"
     CDS             8835..9140
                     /gene="rplX"
                     /locus_tag="ACHU66_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR3389258.1"
                     /translation="MFVKKGDKVRVIAGKDKGVEAVVVTALPKVNKVIVEGVNIVKKH
                     QKPNSENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            9164..9706
                     /gene="rplE"
                     /locus_tag="ACHU66_000964"
     CDS             9164..9706
                     /gene="rplE"
                     /locus_tag="ACHU66_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR3389259.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGDA
                     VSNAKNLEKAAQELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVTVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGMDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            9721..9906
                     /locus_tag="ACHU66_000965"
     CDS             9721..9906
                     /locus_tag="ACHU66_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFR3389260.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCEKCGRPHSVYRKFKLCRVCF
                     RDLAYLGQIPGVTKASW"
     gene            10231..10629
                     /gene="rpsH"
                     /locus_tag="ACHU66_000966"
     CDS             10231..10629
                     /gene="rpsH"
                     /locus_tag="ACHU66_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR3389261.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIATILKNEG
                     FVKNVEFIEDDKQGIIRVFLKYGPNGEKVITNLKRVSKPGLRVYSKREDIPKVLNGLG
                     IAIISTSEGLLTDKQARQKNVGGEVIAYVW"
     gene            11094..11630
                     /gene="rplF"
                     /locus_tag="ACHU66_000967"
     CDS             11094..11630
                     /gene="rplF"
                     /locus_tag="ACHU66_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR3389262.1"
                     /translation="MSRIGNKVITLPAGVELAQNNGVVTVKGPKGELTREFPTAIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTSRANLNNMVVGVSEGFKKELEMRGVGYRAQLAGNK
                     LTLAVGKSHPDEVVAPEGITFEVPTPTQIVVSGINKEVVGQTAAYIRSLRAPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            11719..12075
                     /gene="rplR"
                     /locus_tag="ACHU66_000968"
     CDS             11719..12075
                     /gene="rplR"
                     /locus_tag="ACHU66_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR3389263.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKISGTAARPRLNIFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALAESARENGLKF"
     gene            12094..12588
                     /gene="rpsE"
                     /locus_tag="ACHU66_000969"
     CDS             12094..12588
                     /gene="rpsE"
                     /locus_tag="ACHU66_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR3389264.1"
                     /translation="MAFKDNAVEIEERVVAINRVTKVVKGGRRLRFAALVVVGDRNGR
                     VGFGTGKAQEVPEAIRKAVESAKKNMIEVPMVGTTIPHEVRSEFGGARVLLKPASEGS
                     GVAAGGATRAVIELAGIADVTSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            12603..12785
                     /gene="rpmD"
                     /locus_tag="ACHU66_000970"
     CDS             12603..12785
                     /gene="rpmD"
                     /locus_tag="ACHU66_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR3389265.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNPAIL
                     GMVNAISHLVTVEEVK"
     gene            12921..13361
                     /gene="rplO"
                     /locus_tag="ACHU66_000971"
     CDS             12921..13361
                     /gene="rplO"
                     /locus_tag="ACHU66_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR3389266.1"
                     /translation="MKLHELQPATGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRPGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNAFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITSKGGSVEVI"
     gene            13375..14685
                     /gene="secY"
                     /locus_tag="ACHU66_000972"
     CDS             13375..14685
                     /gene="secY"
                     /locus_tag="ACHU66_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR3389267.1"
                     /translation="MFFKLLKDALKVKLVRSKILFTIFILFVFRVGTHITVPGVNAKS
                     LEALSNLPFLNMLSLVSGNAMRNFSVFALGVSPYITASIIVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLVLAFVQSIGITAGFNALSGAKLTNMPLNWQTYLLIGAILTT
                     GSIIVTWLGEQISEKGYGNGTSMIIFAGIISSLPGTFHEIYIDRFVNIESSRLGESAI
                     FVAALVVLIFFVVYFTTFVQQAEYKLPIQYTKRAQGAPSSSYLPLKLNPAGVIPVIFA
                     GSITAVPTSLIQYFASQNKSAGWLLTVQEYFDYSTAKGMIVYAGLIIAFTFFYTFVQV
                     NPEKTAESLQKSAAYIHGVRPGNGTEQFLSKLLTRLAVIGALFLSFVALLPILAQNLF
                     GLSSSIAFLGTSLIIVISTSIEGIKQLEGYLLKRKYVGFLEITE"
     gene            14779..15420
                     /locus_tag="ACHU66_000973"
     CDS             14779..15420
                     /locus_tag="ACHU66_000973"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR3389268.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEKFNVAHISTGDMFRAAMANQTE
                     MGILAKSYIDKGDLVPDEVTNGIVKERLVQDDIKEKGFLLDGYPRTIEQAHALDENLA
                     DLGIELQGVINIEIDPSKLVERLSGRIIHKETGETFHKVFNPPVGDYKEEDFYQREDD
                     KPESVKRRLEVNIAQGQPIIDHYRAKGLVHDIEGDQDIDLVFQAIDTVLSKLQ"
     gene            15542..15760
                     /gene="infA"
                     /locus_tag="ACHU66_000974"
     CDS             15542..15760
                     /gene="infA"
                     /locus_tag="ACHU66_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR3389269.1"
                     /translation="MAKEDVIEIEGKVVDTMPNAMFTVELENGHQVLATVSGKIRKNY
                     IRILVGDRVTVELSPYDLTRGRITYRFK"
     gene            15785..15901
                     /gene="rpmJ"
                     /locus_tag="ACHU66_000975"
     CDS             15785..15901
                     /gene="rpmJ"
                     /locus_tag="ACHU66_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR3389270.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            15921..16286
                     /gene="rpsM"
                     /locus_tag="ACHU66_000976"
     CDS             15921..16286
                     /gene="rpsM"
                     /locus_tag="ACHU66_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR3389271.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VKDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            16304..16687
                     /gene="rpsK"
                     /locus_tag="ACHU66_000977"
     CDS             16304..16687
                     /gene="rpsK"
                     /locus_tag="ACHU66_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR3389272.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            16732..17670
                     /locus_tag="ACHU66_000978"
     CDS             16732..17670
                     /locus_tag="ACHU66_000978"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR3389273.1"
                     /translation="MIEFEKPTITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSIKIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKKIELDVVGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIADGATFKAVLTVNSGRGYVPAEDNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTLEINTNGTIVPEDALGLSARILMEH
                     LGLFTDLTEVAKSAEVMKEAEVASDDRMLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKTEPEMMKVRNLGRKSLEEVKVKLADLGLGLKKDK"
     gene            17685..18071
                     /gene="rplQ"
                     /locus_tag="ACHU66_000979"
     CDS             17685..18071
                     /gene="rplQ"
                     /locus_tag="ACHU66_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR3389274.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            18382..19237
                     /locus_tag="ACHU66_000980"
                     /pseudo
     CDS             18382..19237
                     /locus_tag="ACHU66_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
BASE COUNT         6175 a         3634 c         4147 g         5290 t
ORIGIN      
        1 agcaaaggag tgactacatg attaaaattt atactgtatc tagttgtact tcttgtaaga
       61 aagcgaaaaa ttggttaaat gctcaccaat tatcttataa tgagcataat cttgcgaagg
      121 aagccattac taaggaagaa atcctaaaca tcttaacaaa aacagagaat ggtattgcaa
      181 gtatcgtatc gtcaaaaaac cgttatgcaa agagtctgga ctttgatatt gaagagttga
      241 gtgtgaacga ggtcattgat ttaattacct ctaacccacg tattttgaag agtccaatct
      301 tgattgatga gaaacgactt caagtgggct ataaagaaga tgatatacgc gctttcttgc
      361 cacgtgcagt gcgcaatgtt gaaaatgctc aagctcgtat gagagcagct ctataagatg
      421 tacaaaagga ctcttttggg gttctttttg cgcatttttt gagaataatt aaaatttgtg
      481 atataatgta aaaaataggg aaagaaggtg taagtatggg atttaccgaa gaaaccgttc
      541 gttttcgcct agatgataca gataaacaag aaatcagcaa gacattgact agtgtttatc
      601 gttcgctaga ggaaaagggc tataatccga ttaaccaaat cattggctat gtattaagtg
      661 gggaccctgc ttatattcct cgctataatg atgcccgcaa tcagattcgt aagcatgaac
      721 gagatgaaat cattgaagaa ttggtgcgct actatttgaa agggaatggg attgacctct
      781 aatgagaata atgggattag acgtcggttc caagacagtt ggtgtagcca tttcagatcc
      841 gttaggtttc acggcccaag ggttggaaat catcccaatc gatgaagaaa agggcgaatt
      901 cggtctggag cgtttgaccg aacttgtaga acagtacaag gttgataaat ttgttgtagg
      961 cttgccgaag aatatgaata atactagtgg tccacgtgtg gaagctagtc aagcttacgg
     1021 tgatttattg actgagcgtt acaaacttcc agttgattat caggatgaac gtttaacaac
     1081 tgttgcagca gaacggatgc tgattgagca agcggatatc agccgaggaa aacgcaagaa
     1141 agttatcgat aaattagcag ctcagctgat tttacaaaat tatttagata gaacatttta
     1201 aaggagattc taatggcaca tcatcatgac catgaacacg accacaacca cgacgaacgt
     1261 gaattgatta ctttggtaga cgaccaaggt aacgaaacac tttttgaaat cttattgacg
     1321 attgacggtc aagaagagtt tggtaagaac tatgttctct tgattcctgc aagtgcagaa
     1381 gaagatgaga acggagaagt tgaaatccaa gcctactcat acattgagaa tgagaatggc
     1441 acagaaggcg atttgcaacc tatcccagaa gacgcaacag cagaatggga tatgattgaa
     1501 gaagtcttca acagctttat ggaagaggaa taaaaaggtc cagtggacct ttttatcatg
     1561 agcatgaaaa caagaaagcg aattaaggtc cggtggacct ttttatcatg agcctgaaaa
     1621 caagaaagtg aattaaggtc cagtggacct ttttgtcatg agcctgaaaa caagaaagcg
     1681 aattaaggtc cggtggagtg aagcagtctg ggggagactg tttcagcccg agttaagaaa
     1741 cagcaaggcg aggaatggat tatgagccag aaaatcaaaa gggaggccag atgaaagata
     1801 gttttacaga aagaagtaag aggctgagtg aggaattaga gaggtgtcta ctggctgata
     1861 aaaatatttt ggtcatatta gacataatgg atagactgaa tttatcagat tgctggctct
     1921 gtgcgggaac tatccgtaat ttcatttgga atcaatacag ttttgatgaa gaaacagatg
     1981 ttgatcttgt gttttttgat gaaaatattt cttatgaaga gacaatggaa atagaaagcg
     2041 atttatatca aaactatcca gcctatcgat gggaattaaa aaatcaagtg tatatgcata
     2101 tccatagtcc gaatactaaa ccgaatcaat catcaaggga tgcgattgaa aaatttccag
     2161 aaagatgcac agcgataggt atttgtcgaa cagaaagaaa taaattagaa atatttgctc
     2221 cttttggatt ggatgacatt tatgcttacc aagtacggcc aacaccacat ttcttagcgg
     2281 atcctaatcg catggaattg tatcgacaaa gaattcttaa gaaacagtgg aaaaagaggt
     2341 ggctatcgct aacagtaaaa gagacttaac tcccaccaag taagtaggcc aaaaacagta
     2401 ttttttataa actaaaacaa tcaactacgg gagattatgt tatggaattt gaaacggttt
     2461 gtttgaaggt tagaaactta ggtaggctat attgctactt caaagttaaa tttagaaacc
     2521 atatcgaggc tataattcgt aaacaggaaa gtcaaaaatg taaatttgat cgtctcttcc
     2581 atgaagatat tcatagtttg attcaatcac cgagcttggg taatgatgag ttgctgatgt
     2641 tatggggtct cctgagagat tatcgaaaaa atttaatcaa tgaccgactt gaaaatatga
     2701 aaaattgata agcggacaga gttttactgg cagtcaaaaa aataattcga gacttacaag
     2761 gatacttgaa aaaaacgttg atagttaact caaataaaaa ataagtaatg aagaatcctg
     2821 aaataaaagt tttgggattt tttgaatttt tgagtaaaaa ctcttgcaat ggcaagtgga
     2881 atctggtaga atgttctagt gccgtaaaaa atacggctgt agctttgatg caagaggttg
     2941 cgacacgctc ggttgcattg ccacgcaaca cgtgttggtt ttcttgtgga gctagcctat
     3001 tatgttaaat agacgaaaag gagaaaaaga tggcaaacaa aaaaattcgc atccgcttga
     3061 aagcgtacga acaccgtaca cttgatacag cagctgcaaa aatcgttgaa actgcaacac
     3121 gtacaggtgc tcaagtagca ggtccagttc cgcttccaac agaacgtagc ctctacacaa
     3181 tcatccgtgc gactcacaag tataaagact ctcgtgagca gttcgaaatg cgtacacaca
     3241 aacgtttgat cgacatcatt aacccaactc aaaaaacagt tgatgctttg atgaaattgg
     3301 ctcttccaag tggtgtaaac gtagaaatca aattgtaatc aagtagcaag gtaaactaaa
     3361 agcaagtgtt cataattgaa cacgggctaa actccgtgtg aaaaagataa actttcctag
     3421 aaacagaagt ttcttcgtca agtttcctat tttcactttg agtttgacgc cctttgtatc
     3481 ttggtttgag catgaaaaac gctcgttaaa aactttttaa ctataaaata agaaaaggaa
     3541 tatttttctc atgacaaaag gaatcttagg gaaaaaagtg ggaatgactc aaatcttcac
     3601 tgaatctggt gaatttatcc ctgttactgt catcgaagca actccaaacg ttgttcttca
     3661 agtgaaaaca gttgaaactg acggttatgc agcagttcaa gttggttttg atgacaaacg
     3721 tgaagtattg agcaacaaac ctgccaaagg ccatgtagct aaagctaaca cagctcctaa
     3781 gcgcttcatt cgtgaattca aaaacattga aggcttggaa gttggacaag aaattacagt
     3841 tgaaactttc gcagctggtg atgttgttga tgtaactggt acatctaaag gtaaaggttt
     3901 ccaaggcgtt atcaaacgcc atggtcaatc acgtggtcct atggctcacg gttctcgtta
     3961 ccaccgtcgt ccaggttcta tgggtcctgt tgcacctaac cgtgtattca aaggtaaaaa
     4021 ccttgcaggt cgcatgggcg gcaaccgtgt aacaattcaa aaccttgaag ttgtacaagt
     4081 tgttccagaa aagaacgtta tccttatcaa aggtaacgta ccaggtgcta agaaatctct
     4141 tatcactatc aagtcagcag ttaaagctgg taaataataa ggaaagggga aatcagtcga
     4201 aatggcaaac gtaacattat ttgaccaaac tggtaaacaa gctggtgaag tagttcttaa
     4261 cgatgcgatc tttggtatcg agccaaacca agcagttgta tttgatgtga tcatcagcca
     4321 acgtgctagc cttcgtcaag gtactcacgc agttaaaaac cgttcagcag tctcaggtgg
     4381 cggacgcaaa ccatggcgtc aaaaaggaac tggacgtgct cgtcaaggtt ctatccgttc
     4441 accacaatgg cgtggcggtg gcgtagtctt cggaccaact ccacgttcat acgcgtacaa
     4501 acttccacaa aaagttcgtc gcttggcact taaatctgtt tactcagaaa aagttgctga
     4561 aaacaaattt gtagctgtta actcgcttga attcacagct ccaaaaactg ctgaatttgc
     4621 aaaagtactt gcagcattga gcattgattc taaagtcctt gttattcttg aagaaggcaa
     4681 cgaattcgca gctctttctg ctcgtaacat cccaggagtt aaagttgcaa ctgcaacaac
     4741 tgcaagcgta cttgacatcg caaatgcaga caaacttctt gtaactcaag cagctatctc
     4801 taaaattgag gaggttcttg cataatgaat ttgtatgatg ttatcaaaaa acctgtcatc
     4861 acagaaagct caatgggcca actcgaagca ggcaagtatg tatttgaagt tgacactcgt
     4921 gcgcacaaac tcttgatcaa gcaagctgtt gaagctgcat tcgagggtgt taaagttgca
     4981 aatgttaaca caatcaacgt gaaacctaaa acaaaacgcg taggtcgtta tgtaggtcgc
     5041 acaaacaaag tgaaaaaagc aatcatcaca ttggctgctg attcaaaagc gatcgaattg
     5101 ttcgctacag ctgacgctga ataatcaaag gaggaattaa cgtgggtatt aaagtttata
     5161 aaccaacgac aaatggccgt cgtaacatga cttctttgga cttcgctgaa atcacaacaa
     5221 gcactccaga aaaaagcttg cttgttgctt tgaagagcaa agctggtcgt aacaataacg
     5281 gtcgcatcac tgttcgtcac caaggtggcg gtcacaaacg tttctaccgt ttggtagact
     5341 tcaaacgtaa caaagatggc gttgaagcaa tcgttaaaac tatcgaatac gatccaaacc
     5401 gttcagcaaa catcgctctt gtacactaca cagacggtgt taaagcttac atcattgctc
     5461 ctaaaggtct tgaagttggt caacgcatcg tttcaggtcc agaagcagat atcaaagttg
     5521 gtaacgcact tccacttgca aacatcccag tcggtactgt tgttcacaac atcgagttga
     5581 aaccaggtcg cggtggtgag ttggttcgtg ctgctggtgc atctgcacag gttcttggtc
     5641 aagaaggtaa atacgttctt gttcgccttc aatcaggtga agttcgtatg atccttggta
     5701 cttgccgtgc tactgttggt actgtaggta acgaacaaca tggccttgtt aaccttggta
     5761 aagcaggtcg tagccgttgg aaaggtatcc gtccaacagt tcgcggttct gtaatgaacc
     5821 ctaacgatca cccacacggt ggtggtgaag gtaaagcacc agttggtcgt aaagcaccat
     5881 ctacaccatg gggtaaacca gctcttggtt tgaaaactcg taacaagaaa gctaaatctg
     5941 acaaacttat cgttcgtcgt cgcaaccaaa aataatctat tcttggttgc ttagcattct
     6001 atatcatccg ccaactcggt agcccgcaaa gcgggcaagc cgctgtggta catattttaa
     6061 aggagaaaac actaaaatgg gacgtagtct taaaaaagga cctttcgtcg atgagcattt
     6121 gatgaaaaaa gttgaagctc aagcaaacga cgaaaagaaa aaagtaatta aaacttggtc
     6181 acgtcgttca acgattttcc caagtttcat cggttataca atcgcagttt acgatggacg
     6241 taaacacgta cctgtataca ttcaagaaga catggtaggt cacaaacttg gtgaatttgc
     6301 accaactcgt acttacaaag gtcatgctgc tgacgacaag aaaactcgtc gtaaataatt
     6361 taggaggaaa acacaatggc agaaattact tcagctaaag caactgctcg cacagtacgt
     6421 gtttcacctc gtaaatcacg tcttgtcttg gataacatcc gtggcaaaag cgtagcagac
     6481 gcaatcgcaa tcttgaaatt cacaccaaac aaagctgcag gcattatcga gggagtttta
     6541 aactcagcaa tcgctaacgc tgaaaacaac tttggtttgg aaaaagctaa cttggtagtc
     6601 agcgaagcat tcgcaaacga aggaccaacg ttgaaacgtt tccgtccacg tgcgaaaggt
     6661 tcagcttcac caattaacaa acgcacagct cacatcactg tagttgtggc agagaaataa
     6721 ggaggtaaaa acgtgggtca aaaagtacat ccaattggta tgcgtgttgg catcatccgt
     6781 gattgggatg ctaaatggta tgctgaaaaa gaatacgcgg attaccttca tgaagatctt
     6841 gcaatccgca actttatcaa aaaagaattg gctgatgcgt caacatcaac aatcgaaatc
     6901 gaacgtgctg taaataaagt aatcgtttct atccacactg ctaagccagg tatggttatc
     6961 ggtaaagctg gtagcaacgt tgatgcactt cgtgctcaat tgaacaaatt gactggtaag
     7021 caagtacaca tcaacatcat cgaaatcaaa caacctgatt tggatgcaca ccttgttggt
     7081 gagtcaatcg ctcgtcaatt ggagcaacgt gtggcattcc gccgtgctca aaaacaggct
     7141 atccaacgtg caatgcgcgc tggtgcaaaa ggtatcaaaa cacaagtttc tggtcgtttg
     7201 aacggtgctg atattgcccg tgcagaaggc tactcagaag gaactgttcc tcttcataca
     7261 cttcgtgcgg atatcgacta cgcttgggaa gaagctttga caacttacgg taaacttggt
     7321 gttaaagtat ggatctaccg tggtgaagtt cttccagctc gtaaaaacac taaaggaggt
     7381 aaataacaaa tgttagtacc taaacgtgta aaacaccgtc gtgaattccg tggaaaaatg
     7441 cgcggtgaag ctaaaggtgg aaaacaagta gactttggtc aatacggtct tcaagcaact
     7501 actagctcat ggattacaaa ccgccaaatc gaagctgccc gtatcgctat gacgcgttac
     7561 atgaaacgtg gtggtaaagt ttggatcaag atcttcccac acaaatcata cactgctaaa
     7621 gctatcggtg tacgtatggg ttctggtaaa ggtgctcctg aaggttgggt agctccagtt
     7681 aaacgcggta aggttatgtt tgaagtagct ggcgtttctg aagaaatcgc tcgcgaagca
     7741 ttccgccttg ctggtcacaa attgccagtt aaagttaaat tcgtaaaacg tgaagcagaa
     7801 taaggagaag acatgaaact tcaagaaatt aaagattttg taaaagaact tcgtggcctt
     7861 tctcaagaag aacttgctaa gaaagaaaac gaattgaaga aagaactctt cgaacttcgt
     7921 ttccaagctg ctgctggtca acttgagcaa actgctcgtt tgaacgaagt gaagaaacaa
     7981 attgcacgta tcaaaactgt gcaatctgaa actaaataat agattgggaa aggagaattt
     8041 caatggaacg caataatcgt aaagttcttg ttggacgcgt agtatctgac aaaatggaca
     8101 aaacaatcac agttgtagtt gaaactaaac gtaaccaccc agtctatggt aaacgtatta
     8161 actactctaa aaagtacaaa gctcatgatg aaaacaatgt tgctaaagaa ggcgatatcg
     8221 ttcgtatcat ggaaactcgc ccactttcag ctacaaaacg tttccgtctt gtagaagttg
     8281 tggaagaggc agttatcatt taatcaacct gaaaggagaa aattgacatg attcaaacag
     8341 aaactcgttt gaaagttgct gacaacagtg gcgcacgtga aatcttgaca atcaaagttc
     8401 ttggtggttc aggacgtaaa ttcgcgaaca tcggcgacat catcgttgct tcagtaaaac
     8461 aagctactcc tggtggtgcg gttaaaaaag gtgacgttgt taaagccgtt atcgttcgta
     8521 ctaagacagg tgctcgtcgt gctgatggtt catacatcaa attcgatgag aatgctgcag
     8581 ttatcatccg tgaagacaaa aaccctcgcg gaactcgtat ctttggccca gtggcacgcg
     8641 aattgcgtga tggcggtttc atgaaaatcg tttcattggc accagaagta ctttaatcaa
     8701 atagttttag gaaatttgtt acaaactaac gaagtctgtg acgggtttag taaactagaa
     8761 aacaaactaa gtcccctggt taaatcacta accagggtgc ccattagggc gtaagaaatt
     8821 ataggagaaa tcaaatgttt gtaaaaaaag gcgataaagt tcgcgtaatc gctggtaaag
     8881 acaaaggcgt tgaagcagtt gtcgtaacag cacttccaaa agtaaacaaa gttattgttg
     8941 aaggtgttaa catcgttaag aaacaccaaa aaccaaacag cgaaaaccct caaggcgcta
     9001 tcgttgaaaa agaagctcca atccatgtgt caaacgttca agttcttgac aaaaatggtg
     9061 ttgcaggacg cgttggttac aagtttgtag acggcaaaaa agttcgctac aacaaaaaat
     9121 caggcgaagt acttgattaa tcacgaagga aaggagaagt ataatggcaa atcgtttaaa
     9181 agaaaaatat cttaatgaag tagttcctgc tttgactgaa caatttaact attcttcagt
     9241 tatggctgtg ccaaaagttg ataagatcgt tttgaacatg ggtgttggtg acgctgtttc
     9301 taacgctaaa aaccttgaga aagctgctca agaattggct ttgatctcag gtcaaaaacc
     9361 acttatcact aaagctaaga aatcaatcgc cggcttccgt cttcgtgagg gtgttgcgat
     9421 cggtgcgaaa gtaactcttc gtggcgaacg tatgtatgaa ttcttggaca aattggttac
     9481 agtttcactt ccacgtgtac gtgacttcca cggtgtacca actaagtcat ttgacggacg
     9541 tggtaactac acacttggtg tgaaagagca attgatcttc ccagaaatca acttcgacga
     9601 tgttgataaa actcgcggta tggatatcgt tatcgttaca actgctaaca ctgacgaaga
     9661 atcacgtgca ttgcttactg gccttggtat gccgtttgca aaataagagg gagagaataa
     9721 atggctaaaa aatcaatgat cgctaagaac aaacgcccag ctaagttctc tacacaagct
     9781 tacacacgct gtgaaaaatg tggacgtcca cactcagttt accgcaaatt caaattgtgc
     9841 cgtgtatgct tccgcgactt ggcttacctt ggacaaattc caggcgtaac aaaagcttct
     9901 tggtaagata agatgtgaga gcgtttagca accaacgcaa agataggaga ttttacaacg
     9961 aagtttactt ctaggatttg ctaagcttgt agctcgaact caattaaaac atgatactaa
    10021 gggcgtgacg gacaaagtga aaataggaac tctagcgaag aacgcttgcg ttctagatag
    10081 agttatcttt ttcacacagg ccgtagcccg agttcaaatg ttaggatgca atagcatcat
    10141 actagcaaat tcaaactttg cattgcctat caggcacaca ttaactagta agtgatctaa
    10201 tcaaactgct agtaagagga gaaacaacaa atggttatga ctgacccaat tgcagatttt
    10261 ttgacacgta ttcgtaacgc taaccaagca aaacacgaag tgcttgaagt acctgcatca
    10321 aacatcaaaa aaggtattgc tacaatcctt aaaaacgaag gttttgtaaa aaacgttgaa
    10381 ttcatcgaag atgacaaaca aggcatcatc cgtgtattct tgaaatacgg accaaacggt
    10441 gaaaaagtta tcacaaactt gaaacgcgtt tcaaaaccag gtcttcgtgt ttactcaaaa
    10501 cgtgaagata ttcctaaagt tcttaacgga cttggtatcg caatcatctc aacatcagaa
    10561 ggtcttttga ctgacaaaca agctcgtcaa aagaacgttg gtggtgaggt tatcgcatac
    10621 gtttggtaat cttttagctt ctaatttcgt catgtgcctg tgttagagcg tcttgacata
    10681 ctcaagtatg gtctgcgtcg ctctgccttg tcacatttag aaattgaaac ctaaaagcat
    10741 gatacaaatg cgagaggacc agttcttcgt gcgtgttcaa atctgactta ctgattattt
    10801 ggtatcatgc aaccaaacta gaacaagatt cactaattga acacgcctac aactctttga
    10861 aaaaagtgaa aagtgtttca gaaatgctca ccttgttcgt cgtaaacgtc tgaactttct
    10921 ctggccttcg gtcaggaaag tcctagtcgt tctgacgagg gtgcgtctgc gaaatcaaag
    10981 acttcggact taccgcttat ttttcttcga gttgcttaac ggctttgtat cttgttcacc
    11041 ccgtgaaaac tagtcgctcg acggcttgat aatttaacag gagaattaaa aatatgtcac
    11101 gtattggtaa taaagtaatt acattgcctg ctggcgttga gcttgctcaa aacaacggcg
    11161 tggtaactgt aaaaggacct aaaggggaat tgactcgtga attcccaact gctattgaaa
    11221 tccgtgtgga aggtacagaa gttactcttc accgtccaaa cgattcaaaa gaaatgaaga
    11281 ctattcacgg tactagccgt gctaacctca acaacatggt tgttggtgtt tctgaaggct
    11341 tcaaaaaaga acttgaaatg cgtggtgtcg gttaccgtgc tcaattggct ggtaacaaat
    11401 tgacacttgc tgttggtaaa tcacatccag atgaagtggt tgcaccagaa ggtattacat
    11461 ttgaagttcc aacaccaaca caaatcgtcg tgtctggtat caacaaagaa gttgttggtc
    11521 aaacagcagc ttacatccgt agccttcgcg ctcctgagcc atacaaaggt aaaggtatcc
    11581 gctacgttgg tgaattcgtt cgccgtaaag aaggtaaaac aggtaaataa tagcttgctg
    11641 aggtggctta gccacctggc tgactatttc tcaaattgtt tcgcaagaaa cagaatcata
    11701 cattaagagg tgaatattgt gatttcaaaa ccagataaaa acaaaatccg ccaaaaacgc
    11761 caccgtcgcg ttcgcggtaa aatctctgga actgctgctc gcccacgttt gaacattttc
    11821 cgttctaata caggcatcta cgctcaagtg attgatgacg tagcgggtgt aacgctcgca
    11881 agcgcttcta ctcttgataa agaagtttca aaaggtacta agacagaaca agctgttgtt
    11941 gtaggtaaac tcgttgctga acgcgcggta gctaaaggta tttctgaagt ggtctttgac
    12001 cgcggtggat atctctatca cggacgtgtg aaagctttgg ctgaatcagc tcgtgaaaac
    12061 ggattgaaat tctaataggg aggacactaa gaaatggcat tcaaagataa cgcagttgaa
    12121 attgaagaac gcgtagtagc catcaaccgt gttacaaaag ttgttaaagg tggacgtcgt
    12181 cttcgttttg cagctcttgt ggttgttggt gaccgtaacg gtcgcgtagg tttcggtact
    12241 ggtaaagctc aagaagtacc agaagctatc cgtaaagcag ttgaatctgc taagaaaaac
    12301 atgattgaag taccaatggt tggtacaaca atccctcacg aagttcgctc agaatttggc
    12361 ggcgctcgtg tattgttgaa acctgcttca gaaggttctg gggttgctgc cggtggtgca
    12421 actcgtgccg taatcgaatt ggcaggtatc gcagatgtga cttctaagtc acttggttca
    12481 aacacaccaa tcaacatcgt tcgcgcaaca gttgaaggtt tgaaacaatt gaaacgtgct
    12541 gaagaagtgg ctgcacttcg tggcatctca gtttctgatt tagcataaga aaggagatac
    12601 tcatggctca aattaaaatc actttgacta agtctccaat cggtcgcaaa ccagaacaac
    12661 gtaaaacagt tgttgcactt ggacttggta aattgaactc ttcagttgtt aaagaagata
    12721 acccagctat tcttggaatg gtgaacgcta tctctcactt ggtaactgta gaagaagtta
    12781 aataatctaa caaatagact aagtcgcata gagaaatctt tgcgacttct agtccatttt
    12841 acatatttac atacagcgag tttgtggagg aagactcttg tacttcacag gcgctagcat
    12901 ataaaagagg agaaataata atgaaacttc atgaattaca acctgctaca ggttctcgta
    12961 aagtccgcaa ccgtgtaggt cgtggtacat catcaggtaa cggtaaaaca tctggccgtg
    13021 gtcaaaaagg tcaaaaagct cgtagcggtg gcggtgttcg cccaggtttt gaaggtggac
    13081 aaactccatt gttccgtcgt cttccaaaac gtggatttac aaatatcaac gctaaagagt
    13141 acgcaatcgt taaccttgat caattgaacg cttttgaaga tggtgcagaa gtaacaccag
    13201 ttgtgcttat cgaagctgga atcgttaaag ctgaaaaatc aggaattaaa attcttggta
    13261 acggtgaatt gacgaagaaa ttgacagtta aagctgctaa attctctaaa tcagctgaag
    13321 aagcaatcac ttctaaaggt ggctcagtag aagtcatcta agaggtggca gcctatgttt
    13381 tttaaacttt taaaagatgc cttaaaggta aaattggtac gtagtaaaat tctatttact
    13441 atctttatcc tttttgtctt ccgcgtagga acccacatta cagtaccagg agtaaacgca
    13501 aagagtcttg aggccttgtc aaaccttccg ttcttgaaca tgttgagttt ggtttcagga
    13561 aatgctatgc gtaatttctc agttttcgca ctaggtgtta gtccttatat tacagcttcc
    13621 atcattgttc aacttttaca aatggatatt ttacctaaat ttgttgaatg gggtaagcaa
    13681 ggtgaagttg gtcgtcgtaa actaaatcaa gcaacacgct acatttcttt ggtactggca
    13741 tttgttcaat caattggtat tacagcaggc tttaatgctt tgtccggtgc aaaattgacc
    13801 aatatgccat taaattggca aacatatttg ttgattggag caattttgac aacaggttcg
    13861 attattgtaa cctggctggg ggaacagatt tctgaaaagg gctatggtaa tggtacatca
    13921 atgatcatct ttgcagggat tatttcatcg ttaccaggaa cttttcacga gatttacatt
    13981 gatcgctttg ttaatattga atcaagtcgt ttgggggaat cagcaatctt tgttgctgca
    14041 ctcgtcgtat taatattctt tgttgtgtat tttacaactt ttgtacaaca agcagaatat
    14101 aaattaccaa ttcaatatac aaaacgtgca caaggagcac cttctagctc atatttaccg
    14161 ctgaaattga atccagcagg agttattccc gtaatctttg caggctcaat cactgcagtt
    14221 ccaacttctt tgattcaata tttcgcaagt caaaataaga gtgccgggtg gttattgacg
    14281 gttcaggaat actttgatta ttcaactgct aaaggtatga ttgtatatgc aggtttaatt
    14341 attgccttta cattcttcta cacctttgtt caagtaaatc ctgagaagac tgcagaaagc
    14401 cttcagaaaa gtgcagccta tatccatgga gttcgtcctg gtaatggcac tgaacagttt
    14461 ttgtcaaaat tattgacaag attggcagta atcggtgcac tcttcttgag ttttgtagct
    14521 ttgctgccta tccttgcgca aaatctcttt gggctttctt caagcattgc gttccttggt
    14581 acaagtttga ttatcgtaat ctctacaagt atcgaaggca tcaagcaatt agaaggctac
    14641 cttcttaaga gaaaatatgt aggtttctta gaaattacag aatagaatat aggttgacct
    14701 agtcaacctt ctattttgtt tttagataag tttgtcaata atggcaaatt tatgtgaaaa
    14761 caaaataagg agtttgtcat gaatctttta attatgggtt taccaggtgc tggtaaaggg
    14821 acacaagcgg ccaagattgt tgagaaattc aatgttgctc atatttcaac aggagatatg
    14881 ttccgtgcag caatggccaa tcagactgaa atgggtattc ttgccaagtc gtacattgat
    14941 aagggtgacc tcgttcccga tgaagttact aatggcattg tcaaagaacg tttggttcag
    15001 gatgacatca aggaaaaagg ctttttgctt gatggctatc ctcgtacgat tgaacaagcc
    15061 catgccttgg atgaaaattt ggctgactta ggaattgagt tgcaaggtgt tatcaatatt
    15121 gagattgatc cttcaaaatt ggtagagcgt ctcagcggtc gtattattca taaagaaact
    15181 ggagagactt tccacaaagt attcaatcca cctgttggag actacaaaga ggaagacttc
    15241 tatcaacgtg aagatgacaa gccagaatct gtcaaacgtc gtttagaggt caatatcgca
    15301 caaggacagc caatcattga tcattatcgt gcaaaagggc tggtccatga tattgaaggt
    15361 gaccaagaca ttgaccttgt tttccaagca attgatacag tactatcaaa attgcaataa
    15421 actaatagat ttggcttgca ttttttatac aaagatgata aaatagccta gtctgactta
    15481 taattgttac ctctgtgttc agaggacatc aaatcgatat ttagagaggg gttacttttg
    15541 catggcaaaa gaagatgtga ttgaaattga aggcaaagta gtcgatacaa tgccaaatgc
    15601 tatgtttact gttgagttgg agaatggaca ccaagtcctt gcaactgttt caggtaaaat
    15661 ccgtaaaaac tacattcgta ttttggtcgg tgaccgcgta actgttgagc ttagtccata
    15721 cgacttgaca cgtggacgta tcacataccg ctttaaatag tcgaaatact tggagggatt
    15781 aaacatgaaa gtaagaccat cggtcaaacc aatttgcgaa tactgcaaag ttattcgtcg
    15841 taatggtcgt gttatggtga tttgcccagc aaaccctaaa cacaagcaac gtcaaggtta
    15901 agaaatagaa aggagaaaac atggctcgta ttgctggagt tgacattcca aatgacaaac
    15961 gtgtagttat ttcattaact tatgtttatg gtatcggtct tgcaacatct aaaaaaattc
    16021 ttgcagctgc aggtatttca gaagatgtac gcgtgaaaga tttgacatca gatcaagaag
    16081 atgctattcg tcgtgaagtt gatgcaatca aagttgaagg tgacctccgt cgtgaagtta
    16141 acttgaacat caaacgtttg atggaaatcg gttcataccg tggtatccgt caccgtcgcg
    16201 gacttcctgt ccgtggacaa aacactaaaa acaatgcccg cactcgtaaa ggtaaagctg
    16261 ttgcgattgc aggtaagaaa aaataatata ggaggtagaa aaattggcta aaccaacacg
    16321 taaacgtcgt gtgaaaaaga atatcgaatc tggtattgct catattcacg ctacatttaa
    16381 taacactatt gttatgatta ctgatgtgca tggtaacgct attgcttggt catcagctgg
    16441 tgctcttggt ttcaaaggtt ctcgtaaatc tacaccattc gcggctcaaa tggcttcaga
    16501 agctgctgct aaatctgcac aagaacacgg tcttaaaaca gttgaagtta ccgttaaagg
    16561 cccaggttca ggtcgtgagt ctgctatccg cgctcttgct gccgctggtc ttgaagtaac
    16621 agcaattcgt gatgtgactc ctgtaccaca caatggtgct cgtcctccaa aacgtcgccg
    16681 tgtataatca tacattccat acacagcttt tcgtttaaga gggagtaaga aatgattgag
    16741 tttgaaaaac caacaataac aaaaattgat gaaaataaag attacggcag atttgtaatc
    16801 gaaccattag aacgtggtta cggtacaact cttggtaact ctcttcgtcg tgtacttctt
    16861 gcctcacttc caggtgctgc agtgacatcg attaaaattg atggtgtact ccacgaattc
    16921 gacacagttc caggtgttcg tgaagatgtt atgcaaatta ttcttaacat caaaggcatt
    16981 gctgtaaaat cttatgtcga agacgaaaag aaaattgaac ttgatgtagt aggtccagct
    17041 gaagtaacag caggagacat tcttacagat agtgacattg aaattgtaaa ccctgaccat
    17101 tatctcttta ccattgctga tggtgccact tttaaagctg ttttgactgt caattcaggt
    17161 cgtggttatg taccagctga ggataataag aaagatgatg caccagtggg aacacttgct
    17221 gtagattcta tctatacgcc agtgaagaaa gtcaattatc aagttgaacc agctcgtgtt
    17281 ggtagcaacg atggttttga caaattaaca cttgaaatca acacaaatgg caccattgtt
    17341 ccagaagatg ctttaggtct ttctgcacgt attttgatgg agcacttagg tctcttcact
    17401 gacttgactg aagttgcaaa atctgcagaa gtgatgaaag aagctgaagt ggcttcagat
    17461 gatcgtatgc ttgatcgtac gattgaagaa ttggatctat ctgttcgctc atataactgt
    17521 ctgaaacgtg caggcattaa cactgtattc gacttgacag agaaaactga gccagaaatg
    17581 atgaaagtgc gcaatcttgg tcgcaagagt cttgaagaag ttaaagttaa attggctgat
    17641 cttggtctag gattgaaaaa agataaataa tataggagga aatcatggca taccgtaaac
    17701 taggacgcac tagctcacaa cgtaaagcaa tgttgcgcga tttgacaact gaccttttga
    17761 tcaacgaatc aatcgttaca actgaagctc gtgctaaaga aatccgtaaa acagttgaaa
    17821 aaatgatcac acttggtaaa cgtggtgatt tgcacgcacg tcgtcaagca gcagcatttg
    17881 ttcgtaacga aattgcatct gaaaactatg atgaagcaac tgataagtac acttctacta
    17941 cagcacttca aaaattgttc tctgaaattg cacctcgtta tgcagaacgt aatggtggat
    18001 atactcgtat cctcaaaact gaaccacgtc gtggtgatgc tgcaccaatg gcaattatcg
    18061 aacttgtata agatcatcaa ttttgttgag tgttatgatg atggaatcct attctgattc
    18121 ttagtctagc tctggtctac cgctgagctt atctcagcgg taacactcat catgttgatt
    18181 ggacgcttgt ttacgaaatt actctaagat agaataattt cgtaagcagg cgttttttaa
    18241 tagatgatac atatcagaaa cagagcattt acaagaagaa cgaatcaata gttactcgtg
    18301 gtgctagttc atttcaaaat acaataaaaa gcccaaatgg actatactgt aagtgacgaa
    18361 acatacagga ggttagtcca aatgagccac ttacagtata ccgctaaatc tcatcatttg
    18421 caatggaatg tacgccaatt atcacaaatt tgtcatcact tctacaacaa ctattgcccg
    18481 gattccttca aatatcggcg aaatgttggc ttagttaaag tttcagatga atcaatcctt
    18541 gtcttacttt tgttacaagc tgaactaggt atgacatcac agcgccattt ctacaccatc
    18601 tgccacctct tcccttgcgg tcagttacta gaaagaagcc gtttcaatcg tcggtcaaaa
    18661 caattgattt ggctcgtcca atcaatccga aaagctatga gtgaaatgct tccatctggt
    18721 aaactagcca ttatagatag ttttcctttg ccaacctgtt cgcaatcaca gagcaaccat
    18781 ttttaaagga ttagccgata ttggctacaa cgcctctaaa catctttggt tctacggctt
    18841 caaagttcat atgctagtga ctttatctgg ttatattttg aactatgttg tcacgccagc
    18901 ctctgtccat gatatcaagg tggtttatga gctattagag gggtgtaagc aatcggttat
    18961 cttaggtgat ctaggctacc taagcagtga gctcaaaaag gacttagagc aagaaggtta
    19021 ccatctatgg acgccgtttc gtaaaaacat gactgggtcc gaagaacata acaattggaa
    19081 agtaatggcc atgagacgaa ctgttgaaac acgtttttct gaactgtgtc gtctctttga
    19141 catcgaacat acattagcta gaagcctagc tggactacag ttacgaattg aacaaattat
    19201 tctagctcat aacttacgct attttgaaat gaactagcac cacggg
//
LOCUS       DAZQCJ010000030        18782 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000030 DAZQCJ010000000
VERSION     DAZQCJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18782
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.030"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(130..2463)
                     /locus_tag="ACHU66_000981"
     CDS             complement(130..2463)
                     /locus_tag="ACHU66_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFR3389275.1"
                     /translation="MNNKIIETLEFHKVRQKIEPYLLTEQGFEELRQLEPMVEVQRIQ
                     QAFDELTDIAQIFVENPYFSLSATSDIGPAMRRLELDTDLNIAELLAVKKVLEVSKSL
                     LDFYGNLENVSLSQLDKLFEKIELFPHLQGSLQSINDAGFVEDFASEKLARIRRKIRE
                     AEDQVRQVMQDILKTKGDMLSDSILASRNGRNVLPVKNTYRNKIAGVVHDISASGSTV
                     YIEPRAVVTLNEEISHLRAEERHELNRILQELSDMLRPHSGVIRNNAWLIGHIDFVRA
                     KHLFAREHQAVVPKLSEKQDIALLNVRHPLIAKPVPNDLYFGSQLTAIVITGPNTGGK
                     TIMLKTLGLTHLMAQSGLPILADKGSRVAIFKEIFADIGDEQSIEQSLSTFSSHMTHT
                     VQILAEADQDSLILFDELGAGTDPQEGASLAMAILDDLRLRGIKTMATTHYPELKAYG
                     IETSGIENASMEFDTNSLRPTYKFMQGVPGRSNAFEIARRLGLSDIIIQSAQSWTDTD
                     SDVNRIIEKLESQTVESRQRLDKIRDVEQENYKMNRALRKLYDELNRERENELNKARL
                     EAKEIVDMALAESEDILKNLHAAASLKPHQIIEAKAELKKLAPEVVDLSKNKVLKKAK
                     IQREAKVGDDIIVTAYGQRGTLTNQLKDGRWEAQVGLIKMTLAKDEFELVKAEKAEQP
                     KKRQVHTVKRANVRGPKARLDLRGKRYEEAMMELDEFIDQALLNNLAQVDIVHGIGTG
                     VIREGVTKYLRRNKQVKEFGYAPQNAGGSGCTIVTFK"
     gene            complement(3014..3562)
                     /locus_tag="ACHU66_000982"
     CDS             complement(3014..3562)
                     /locus_tag="ACHU66_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFR3389276.1"
                     /translation="MISLAIILILAWSFYIGYSRGLVLQAFYSMGSIIALVVATATYK
                     KLASFLYLWVPFANATQGSSNYYFDEKYLFDLDMVFYAGLAFLLIYVLVYAIVRFIGI
                     FVHLLEAFNPDTKTTNLISGALAVLVTFISLQIVMVLLSTIPLAMIQDKLHSSFLANI
                     MIQYTPFTSSFLKSLWLSNIAG"
     gene            complement(3559..3870)
                     /locus_tag="ACHU66_000983"
     CDS             complement(3559..3870)
                     /locus_tag="ACHU66_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389277.1"
                     /translation="MANLNRYKFTFGEKQLTLTTEHDNLFMEEIERIAQEKYQAIKEK
                     MPTADPETLALLLAINALSVQLTREMAFEQKEQELASVKEKVLKKNVTLIDLDELEDK
                     V"
     gene            complement(3928..4641)
                     /gene="treR"
                     /locus_tag="ACHU66_000984"
     CDS             complement(3928..4641)
                     /gene="treR"
                     /locus_tag="ACHU66_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFR3389278.1"
                     /translation="MKKYQEIYNDLKEKIRTNVYPAESSLPTEQQLQEIYGVSRDTVR
                     KALAILTEGGLIQKVQGRGSMVLKQEILNFPVSGLTSYQELTNVLQLSTKTDVVSLDM
                     ITVNSSLSHLTGFEPYSKVWKVVRTRSIDGKVSVVDTDYLAVDVVPELTTAIAEKSIY
                     EYLENKLGLDIAYAQKEITVEPTNREERELMQSKDDYLVLIKSRVYLGDTQQFQYTES
                     KHKIDKFRFVDFARRKHSL"
     gene            4874..6868
                     /gene="treP"
                     /locus_tag="ACHU66_000985"
     CDS             4874..6868
                     /gene="treP"
                     /locus_tag="ACHU66_000985"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFR3389279.1"
                     /translation="MGKFEKEAKQLLEAIGGKENVNAVTHCATRMRFVLADEKKANVK
                     VIEDIPAVKGTFTNAGQFQVIIGNDIPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNAI
                     QRVMTMLAEIFTPIIPALIVGGLILGFRNVLEGVQMEWLGQAMKDGQLVFDADGNPVW
                     NTIVQVSPFWNGVNHFLWLPGEAIFHFLPVGITWSVSRKMGTSQILGIVLGICLVSPQ
                     LLNAYAVPGTDAATIASDWSWNFGAFSIARIGYQAQVIPALLAGLALSYLEIFWRKVV
                     PEVVSMIFVPFLSLLPAIILAHTVLGPIGWTIGQWLSTVVLAGLTGPLKWLFGAIFGA
                     LYAPFVITGLHHMTNAIDTQLIADAGGTGLWPMIALSNIAQGSAVFAYFLMNRHNEKE
                     AQVSLPATISAYLGVTEPALFGVNVKYVYPFVAAMIGSSIAGLLSVTFNIQAASIGIG
                     GLPGILSIKAEYWGAFFMTMIVAIVVPMILTAVFKRTGAFSKKEETVEEVVAPTEAVV
                     ETGAAIELISPLTGQTKELSQATDPVFASGVMGQGVLIDPTEGLLVAPVDGEISVLFP
                     TNHAVGITATNGVEFLMHIGMDTVGLEGKGFTAHVKQGDKVKAGDKLISFDIDVIKAA
                     GLVAETPVIVTNQTDFDTQVIGNLPRAISQGEAILTVTKN"
     gene            6949..8574
                     /gene="treC"
                     /locus_tag="ACHU66_000986"
     CDS             6949..8574
                     /gene="treC"
                     /locus_tag="ACHU66_000986"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR3389280.1"
                     /translation="MTIDKRKVVYQIYPKSYKDTTGNGVGDLRGIIEKLPYLKELGID
                     MIWLNPFYPSPQRDNGYDISDYTAVNPDFGTMADFEEMVTVGKELGIEFMLDMVLNHC
                     STDHEWFQKALAGDKYYQDFFILRDQPTDWVSKFGGNAWAPFGDTGKYYLHLFDVTQA
                     DLNWRNPHVREELFKVVNFWKDKGVKGFRFDVINLIGKDEVLEDCPINDGKPAYTDRP
                     ITHDYLKMMNNATFGAEKGFMTVGEMSATTIENCILYTAPEREELSMAFNFHHLKVDY
                     KDGQKWTIMDFDFEELKQLFHTWGEEMSAGNGWNALFYNNHDQPRALNRFVDVENFRK
                     EGATMLAASIHLSRGTPYVYMGEEIGMIDPDYDSMDDYVDVESINAYQMLLDQGHAPE
                     QAFKIIQAKSRDNSRTPMQWDASDNAGFSTGTPWLKAGKSYPTINVEAEKTGPIFTFY
                     QELIRLRKELPLISEGDYKAAYKDSQKVYAFERLLNGEKLLVLNNFFAEEVELDLADD
                     YAHGQVLISNYPDNKLGKKITLKPYQALAIQVK"
     gene            complement(8987..10162)
                     /locus_tag="ACHU66_000987"
     CDS             complement(8987..10162)
                     /locus_tag="ACHU66_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HFR3389281.1"
                     /translation="MKRKRTNKPQHMRRKRKTPIMKNNKKMLYTSSLALSLFSTGMIS
                     TNVLAIEWAPRTVSEISPEIVQEEGKMTYTVQYGDTLSAIALAMNIDMDLLAKINQIA
                     DVNLIFPDTVLTTTVDQNNQVTQVEIEAPVQGNTNETVQAIVDLTTNQVTVEDTVVPL
                     DQISSVTDSAPVEEVVEQPVAEASVEEVVEQPVVEAPVEEVVEQPVAEASVAEASVEE
                     VVEQPVVEAPVEEVVEQPVVEAPVEEVVEQPVVEAPVEQPVVETPQVTALSTTTTSTS
                     AYDVGLQPQVAAFRAEVANAFGITSFSGYRPGDSGDHGKGLAIDFMVPESSALGDQVA
                     AYAVANLASKNINYIIWKQRFYAPYDSIYGPAYTWNLMPDRGSVTENHYDHVHVSFN"
     gene            complement(10511..11494)
                     /locus_tag="ACHU66_000988"
     CDS             complement(10511..11494)
                     /locus_tag="ACHU66_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR3389282.1"
                     /translation="MEVQMKYKWATLGTGVIANELVQALQAMVGNLYSVANRTYDKGV
                     EFAQKYGIEKVYREIDEVFEDPEVDIIYISTPHNTHINYLRKALKAGKHVLCEKSITL
                     NSEELAEAIQLAEENQVILVEAMTIFHMPIYRQLSEVVASGKLGDLKMIQMNFGSYKE
                     YDMTNRFFNKNLAGGALLDIGVYALSFVRWFMTEKPNQVLSQVKLAPTGVDEQVGILL
                     SNDAGEMATIALTLHAKQPKRGTIAYDKGYIELYEYPRGQKAVITYTEDGSQEVIEAG
                     DTAKALSYEVADMEKAVAGIENTMHLAYTQDVMEIMTQLRKEWGLVYPEEM"
     gene            11579..12469
                     /gene="rnhC"
                     /locus_tag="ACHU66_000989"
     CDS             11579..12469
                     /gene="rnhC"
                     /locus_tag="ACHU66_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFR3389283.1"
                     /translation="MNTVVLKASAQQKQAMMAHYDRYRQTSKNPYIEAFFKLTGASLS
                     IYTSGKVVFQGEMAEQEAGLWGYEPESSGQTTVPGQNLPMIGTDEVGNGSYFGGLAVV
                     ASFVRPEDHAFLKSLGVDDSKKMTDQKICQVAPLLKEKIPHQALLLSPKKYNEVIEQG
                     YNAVSVKVALHNQAIFLLLQKGVQPEKIVIDAFTSSQNYQKYLKKEANQFANPVTLEE
                     KAEGKYLAVAVSSIIARAMFLENLVQLGQLVGMNLPSGAGSKSDQVAASILKQYGMAG
                     LNQTAKLHFANTQKAQKLLK"
     gene            12479..13108
                     /gene="lepB"
                     /locus_tag="ACHU66_000990"
     CDS             12479..13108
                     /gene="lepB"
                     /locus_tag="ACHU66_000990"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR3389284.1"
                     /translation="MGAIQKTKRSPLVAFLAEWGIFLLFMAAFFASRYFIWNPVSVDG
                     HSMDPTLQHQEKLIMLKTTSIDRFDIVVASETDSDGKEKLIVKRVIGMPGDTIRYEND
                     VLYVNDQKVDEPYLDEYLAAFQKDKLQEVYSYNKQFQAVAQSAEAFTQDANGYVDFTV
                     TVPEGQYYLMGDDRLVSLDSRSVGTFSRENIKGEVVFRMWPLNRIGTVD"
     gene            13172..15661
                     /locus_tag="ACHU66_000991"
     CDS             13172..15661
                     /locus_tag="ACHU66_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFR3389285.1"
                     /translation="MNEVYFTGTIDRIIFENPSNFYKILLLEIEETDADYDDYEIIVT
                     GTIADVIEGEDYRFYGNLVTHPKYGQQLQISRYERSKPTSAGLVKYFSSEHFKGIGRK
                     TAEKIVELYGEDTIDKILAEPEKLTQITGLSSKNMQAFVEKLRLNYGTELILAKLAEY
                     GIPNKLAFQIQDQYKEKTLQIIEENPYQLVEDVQGLGFTIADRIAENLGIASDSPQRF
                     RAGMLFSLIHRSMETGDTYVEARDLLEATLELLEKSRHTELDPAAVAKELTGLIADDK
                     VQQEGTKIFDNSLYFAEHGIHKNLTRLMGKNGFKPFPRTDVEAAIAELETMSSLTYDD
                     IQKEAIVQAITNPLFILTGGPGTGKTTVINGIIAVYAILHKIDLTRNREECPVLLAAP
                     TGRAARRMNELTGLPSATIHRHLGLVEGQEESYRDDYLDADFIIVDEFSMVDTWLANQ
                     LFQNISSQTQVLIVGDAEQLPSVSPGQVLADLLKIDKLPSITLERIYRQSDDSTIVTL
                     ASQIRQGALPSDFREKKADRSYFEAQNEQIPALIERIVGAAIKSGIPANEVQILAPMY
                     RGAAGIDQLNTMTQALLNPLEEGELEFLHNEQAFRQGDRVIHLVNDAEANVFNGDLGY
                     ITDLLPAKYTDSKQDEITINFDGSEVTYPRNEWYKITLAYAMSIHKSQGSEFQVVILP
                     ITRTSHRMLQRNLVYTAITRSKSKLILLGEISAFDYAVKNAGTVRKTYLVPRFQGEMA
                     EQESKESLIKKDESKTATTTQPQPPTQPDRKEQVKVVKENNQQLSLLDQDQPKKTSPQ
                     PTEYILTVDNLLTIDPMIGIEQADIEEFFKA"
     gene            complement(15700..17761)
                     /locus_tag="ACHU66_000992"
                     /pseudo
     CDS             complement(15700..17761)
                     /locus_tag="ACHU66_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636049.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            complement(17934..18743)
                     /locus_tag="ACHU66_000993"
     CDS             complement(17934..18743)
                     /locus_tag="ACHU66_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HFR3389286.1"
                     /translation="MIEYYNFNKSELEKHSRIPLIVKKNNEEIFISLALEMVELIKEN
                     NEKNEKTVIICPVGPVGQYPHFVRLVNQFKIDLSNVWFINMDEYLLEDLHWIDVVHPL
                     SFRGFMQKNVYSQIHPTLIMSEEQRIFPDPLDIDSISNKIKELGKIDLCIGGIGLNGH
                     IAFNEPDSTLTVQEFLKLGSRVLPISVETKVMNGLTVLKGAVEEVPNYCVTIGMSEIF
                     QAKKIRLAVFRDWHHAVIRKTVCSKPDTGFPSTLMQIHTNCSIYLNESLAN"
BASE COUNT         5322 a         4395 c         3797 g         5268 t
ORIGIN      
        1 tatttcttac ctcataagta tagtatagca taacctagta agtacttgaa ctgtattttg
       61 acctatccta ttccctgtat aacagaaata taaaagcaag aacagactga atctatcctt
      121 gctaattcct tatttaaacg tcacaatagt acagcctgat ccacctgcat tttgtggggc
      181 atagccgaac tccttgacct gcttgtttcg gcgtaggtac ttggtcaccc cttctcggat
      241 gacacctgta cctataccgt ggacaatatc gacttgggct aggttattga gaagggcctg
      301 atcgataaat tcatccagct ccatcatagc ctcttcgtag cgtttgccac ggagatctaa
      361 gcgggctttt ggtcctcgta cattggcacg tttgactgtg tgaacctggc gtttcttagg
      421 ctgctctgcc ttctccgcct tgactaactc aaactcatct ttggccaagg tcatcttaat
      481 caagccaacc tgagcttccc aacgcccgtc cttgagctga ttggtcaagg tcccgcgttg
      541 tccataagct gtaacgataa tgtcatcgcc caccttggct tccctctgaa ttttagcttt
      601 cttcagaacc ttgtttttag acagatccac cacttcaggc gccaactttt tcagctctgc
      661 cttggcttcg ataatctggt ggggcttaag actagctgca gcatggagat ttttgagaat
      721 atcctcgctt tctgccaatg ccatatctac aatttccttg gcttccaagc gtgccttgtt
      781 gagctcattt tccctctcac gattgagctc gtcatagagc ttgcggaggg ctcggttcat
      841 cttgtaattt tcttgctcca catcacgaat cttatccaga cgctgacgac tttcaaccgt
      901 ctgactttcc aatttctcga taatgcggtt cacatcgcta tctgtatccg tccaagattg
      961 ggctgactgg ataataatat cagatagacc aagacggcga gcaatttcaa aggcattgga
     1021 gcgaccagga acaccctgca taaacttata ggtcggacgc aggctattgg tatcaaactc
     1081 catgctggca ttttcaatac cagaggtttc tatcccgtag gccttaagct cagggtagtg
     1141 ggtggtcgcc atggtcttga tcccccgcag acgaaggtca tccaaaatag ccattgccaa
     1201 ggacgcaccc tcctgcggat ctgtcccagc tcccaactca tcaaatagaa tgagggaatc
     1261 ttgatccgcc tccgccaaaa tctgtaccgt gtgggtcatg tgactggaga aggtcgataa
     1321 actctgctcg atggactgtt cgtctccaat gtccgcaaaa atttctttga agatagctac
     1381 ccgactgccc ttgtcagcca agataggcaa accagactgg gccatcagat gggtcaatcc
     1441 caaggtctta agcatgatgg tcttaccacc tgtgttggga cctgttatca cgatagccgt
     1501 caactgacta ccgaaataaa ggtcatttgg tacaggctta gcaatgagcg gatgtcgaac
     1561 gttgagaaga gcaatatcct gcttctcaga tagtttgggt acaactgctt gatgttcacg
     1621 tgcaaagaga tgcttagcac ggacgaaatc aatatgcccg ataagccagg cattgttacg
     1681 gatcacacca ctatgcggac ggagcatatc cgataattcc tgcaggatac ggttgagctc
     1741 atggcgttct tctgcacgta ggtgactgat ttcttcgttc aaggtcacca ctgcccgcgg
     1801 ctcaatataa accgtcgagc cagaagcgga gatatcatgg actacacccg caatcttatt
     1861 gcgataggta tttttaacag gaaggacatt gcgtccatta cggcttgcca aaatactatc
     1921 ggacagcatg tcgcccttgg tcttcaaaat atcctgcatg acctgacgaa cttgatcttc
     1981 agcttcacgg atttttcttc taatacgagc cagcttttct gaggcaaaat cctctacaaa
     2041 accagcatca ttgatggact ggagggagcc ctgtaaatgt ggaaaaagct caatcttctc
     2101 aaagagttta tccagctggc taagactgac attttccaga tttccataaa aatccaagag
     2161 agatttagaa acttccaaga cctttttaac agctaataat tcagcgatat tgaggtctgt
     2221 atccaattcc aaacgacgca tggctggacc aatgtcacta gtagctgaca gactgaaata
     2281 gggattttcc acaaaaatct gcgctatgtc tgtcaactcg tcgaaggcct gttggatacg
     2341 ttggacttcc accatgggct ccaactgtcg taattcttca aagccctgtt ccgtcaagag
     2401 atagggctca attttttgtc ttaccttgtg aaattcaagg gtttcaataa ttttattatt
     2461 catatgttcc tacttagtct tttccttagc tttatctcat tatcaaaaca ataaaagata
     2521 aaaatatagt ctttcccaat ctcttacatt aaagttataa cagtcaacta atactcaatg
     2581 aaaatcaaaa tcagacttgc tccaagggtt cgtgggaacc tttagaggtt ggaaataggc
     2641 gaacgtagtt cgtttcgctc tgatgcagat agaactctgt tccttttata tgtcaaggga
     2701 acaggctgag gctgggcaga aagtccatcc tcgcttctca gaattcgtgt caacatctca
     2761 gcgcagtggt tgattggcag atttgttcgt gttccacact ccaaatccga cctaatcaac
     2821 tgtgcggggg tgggagtgaa acagtctggg gatagactgt ttcagctcaa caacttaaaa
     2881 cgaaaaattg ttgacgaact cttttagact gctcgagttc tttcccactc ccactcatca
     2941 aatcaagtca acaatgtctg attttgattt tcgaagagta tcataaatga cgataaaaag
     3001 gataaggtct cccttaccct gctatattac tcaaccagag agattttaaa aagctactag
     3061 tgaatggtgt gtactgaatc atgatgttgg caagaaaact actgtgcaat ttatcttgaa
     3121 tcatcgctaa cggaatagtt gatagcaaga ccatgacaat ctgaagagaa ataaaagtca
     3181 ccaagacagc caaggcgccg cttatgagat tggtcgtttt tgtatcagga ttaaaagcct
     3241 ctaatagatg gacaaatatt cctatgaaac gtacaatagc ataaaccaag acgtatatca
     3301 aaagaaaggc caatcccgca taaaatacca tatctaggtc aaagagatat ttctcatcaa
     3361 aataatagtt ggagctcccc tgagtagcat tggcaaatgg cacccagaga taaaggaagg
     3421 atgccaattt tttataagta gccgttgcaa ccaccagagc aataatactt cccatgctat
     3481 aaaacgcctg tagaactaga ccacggctgt agccaatgta gaaactccat gccaaaatga
     3541 gaataattgc taatgaaatc ataccttgtc ctcaagctca tctaagtcaa tcaaggtcac
     3601 attctttttc aagacctttt ctttgacgct tgcaagttct tgttcctttt gttcaaatgc
     3661 catctcgcgc gttagttgaa cggatagagc attgattgcc aacaaaagcg cgagggtttc
     3721 agggtccgcc gtcggcatct tttctttgat ggcttggtat ttttcctgtg caatacgctc
     3781 aatctcttcc atgaataaat tatcatgctc agttgtcaaa gttaattgct tctcgccaaa
     3841 tgtaaattta tatcgattta agtttgccat atcttacctc ttcttctatc tattatagcg
     3901 taaaaaactt gatttggcta atctctccta taaagaatgc tttctacgag caaaatctac
     3961 aaagcggaat ttatcaattt tatgcttgct ttctgtatat tggaattgtt gggtatcacc
     4021 gagatagaca cgagatttaa tcaagaccaa ataatcatcc ttcgattgca ttagctcacg
     4081 ctcttctcga ttggtcggct ctaccgtaat ctctttttgt gcataagcaa tatcaaggcc
     4141 taacttattt tctaggtatt cgtagatgga tttttcagca atagcagttg tcaactctgg
     4201 cacgacatcg acagcaagat aatctgtatc cacaacggag accttaccgt caattgaacg
     4261 tgtacggaca actttccaca ccttgctata cggctcaaag cctgtcaagt gcgaaaggct
     4321 actattaacg gtaatcatat ctaagctgac aacatctgtc ttggtagaaa gctggagaac
     4381 atttgttaat tcctgatagg aagttaaacc tgaaactggg aaattgagaa tttcttgctt
     4441 aaggaccatt gaaccacgcc cttgcacttt ttgaatcaaa cctccctcag tcaaaatcgc
     4501 caacgcctta cgaaccgtat cacgactaac accatagatt tcctgaagct gctgttctgt
     4561 cggtagggag ctttctgccg gataaacatt tgtccgtatt ttttctttta agtcattata
     4621 aatttcttgg tattttttca tcatttcctc gcaaattcat ctgtcaaata acatacaact
     4681 atcaacatta taacaaaatt catttcaaaa ttaaaaaaac taagcaaaaa cgcttgcatt
     4741 attttttgaa atcggataca atgataacac aaaagaaatt tgtccgtaca aatttcaaaa
     4801 aagacatact tttgggagat gtctaaaaaa tatagggtga cggattccat aagccgtcta
     4861 gaaggaaaaa tatatgggaa aatttgaaaa agaagccaaa cagctcctag aagctattgg
     4921 cggaaaagag aatgtcaatg cagtaacaca ctgtgcaaca cgcatgcgct ttgttcttgc
     4981 agatgagaaa aaagcaaacg taaaagtcat tgaagacatt ccagcagtaa aaggaacatt
     5041 tacaaacgca ggtcaattcc aagtcatcat cggtaacgat ataccaatct tctacaatga
     5101 ctttacagct gtgtctggta tcgaaggcgt atcaaaagaa gctgcaaagt cagccgctaa
     5161 aagtaaccaa aatgccatcc aacgtgtcat gaccatgctg gcggaaatct tcacaccgat
     5221 cattcctgcc cttatcgtcg gcggtttgat tctcggtttc cgtaacgtct tggaaggtgt
     5281 tcagatggaa tggcttggac aagctatgaa agatgggcag ttggtcttcg atgctgatgg
     5341 taaccctgta tggaacacca tcgttcaggt ttctcctttc tggaatggtg tcaatcactt
     5401 cctctggttg ccaggtgaag ccatcttcca cttcttgcca gtaggtatca cttggtccgt
     5461 atctcgtaaa atgggaactt cacaaatcct tggtatcgtc cttggtatct gtttggtttc
     5521 tccacagttg ctcaacgctt atgctgtacc tggtacagat gcagcaacca ttgccagcga
     5581 ctggtcttgg aacttcggtg catttagtat tgcccgtatc ggttatcaag cacaagttat
     5641 ccctgccctc cttgctggtt tggcactgtc ttacctagaa atcttctggc gtaaggttgt
     5701 tccagaagtg gtctcaatga ttttcgtacc attcctatca cttcttccag caattatctt
     5761 ggctcacact gtccttggtc ctatcggttg gacaatcggg caatggttgt caacagttgt
     5821 attagctggt ttgactggtc cattgaaatg gctcttcggt gcgatattcg gtgccctcta
     5881 tgcaccattc gttatcactg gtcttcacca catgacaaat gccatcgata cacaattgat
     5941 tgcggatgct ggtggaactg gtctctggcc aatgattgcc ctttctaaca ttgctcaagg
     6001 ttcagcagta tttgcctact tcctcatgaa ccgtcacaat gaaaaagaag cgcaagtttc
     6061 tcttcctgca actatttctg cttatcttgg tgttactgaa ccagccctct ttggtgtcaa
     6121 cgtgaaatac gtttacccat tcgtagctgc tatgattggt tcaagtatcg caggcttact
     6181 ctctgtaacc ttcaacatcc aagcagcttc tatcggtatc ggtggtcttc caggtatctt
     6241 gtctatcaaa gctgaatact ggggtgcatt ctttatgaca atgattgttg cgattgttgt
     6301 cccaatgatt ttgacagctg ttttcaaacg tacaggtgca ttttcaaaaa aagaagaaac
     6361 tgttgaagaa gttgtagctc caactgaagc agttgttgaa actggcgctg ctatcgaatt
     6421 gattagccca cttactggtc aaaccaaaga attgtcacaa gctactgacc ctgtatttgc
     6481 atcaggtgtc atgggccaag gtgtccttat tgacccaacg gaaggtcttc tggtagcacc
     6541 tgttgacgga gaaatttctg tcctctttcc aactaaccat gctgttggta tcactgctac
     6601 aaacggagtt gagttcctta tgcacattgg tatggatact gttggattgg aaggtaaagg
     6661 ctttacagcc catgtcaaac aaggtgacaa ggttaaagca ggtgacaaat tgattagctt
     6721 tgacatcgat gtcatcaagg ctgctggtct cgtcgcggaa acaccagtta tcgtgaccaa
     6781 ccaaactgac tttgacactc aagttatcgg aaatcttcct cgtgccatct cgcaaggcga
     6841 agctattctg acagttacaa agaattaatg ttctaggagg gcgcaaggta acttgcacct
     6901 cctatcattg cttatgcaag aaggaaaaat caagaaagag agagcaaaat gacaatagac
     6961 aaacgaaagg ttgtttatca aatctaccca aaatcttaca aggatacaac tggaaatggt
     7021 gtaggggatt tacgaggaat tattgaaaaa ctcccatatt taaaagagct aggtattgat
     7081 atgatttggc tcaacccttt ctacccaagt cctcaacgag acaacggtta tgacatttca
     7141 gattatacag ccgtcaatcc tgattttggc accatggctg actttgaaga aatggtgact
     7201 gtcggcaaag aactaggcat cgaattcatg ctagacatgg tcctcaatca ctgctcaacc
     7261 gaccacgaat ggttccaaaa agcccttgcc ggcgacaaat attaccaaga tttctttatc
     7321 ctgcgtgacc agccaactga ctgggtgtcc aagtttggtg gcaatgcctg ggctcctttt
     7381 ggagatactg ggaaatacta cctccaccta tttgatgtga cccaagctga cctcaactgg
     7441 cgaaaccctc atgtccgtga agaactcttc aaggtggtca acttctggaa agacaagggt
     7501 gtcaaaggct tccgcttcga tgtcattaac ctgattggta aggacgaagt cctcgaagac
     7561 tgcccaatca atgatggcaa accagcttat accgaccgcc caattaccca cgactacctc
     7621 aagatgatga acaatgccac ttttggggca gaaaaaggct tcatgaccgt cggggaaatg
     7681 tctgccacca ccattgaaaa ctgcattctc tacacagctc ctgagcggga agaattgtcc
     7741 atggccttca acttccacca cttgaaagta gactacaagg acggtcaaaa atggaccatt
     7801 atggacttcg actttgaaga actcaaacaa ctcttccaca cttggggcga agagatgtcc
     7861 gccggaaatg gctggaacgc ccttttctac aacaaccacg accaacctcg tgccctcaac
     7921 cgctttgttg atgtggagaa tttccgcaag gaaggagcaa ccatgctggc agcctctatc
     7981 cacctgtcac gcggtactcc ttacgtctac atgggcgaag aaatcggcat gattgacccc
     8041 gattacgaca gtatggacga ctatgtggac gtggaatcta tcaatgccta tcagatgctc
     8101 ttggaccaag gtcacgcacc tgagcaggcc ttcaagatta tccaggccaa gtcccgcgac
     8161 aattcccgca ctccgatgca atgggacgct tctgacaatg caggcttctc aacaggcacc
     8221 ccttggctaa aggctggcaa atcctatcca accattaacg ttgaagccga aaaaacaggc
     8281 ccaatcttta ccttctatca agaactcatc cgcctgcgga aagaactccc tctgatttca
     8341 gaaggggact acaaggcagc ctacaaggat agccagaaag tctacgcctt tgaacgcttg
     8401 ctaaatggcg aaaaactgtt ggtcctcaac aatttctttg cagaagaggt tgaactagat
     8461 ttggcagacg actatgccca cggccaagtc ctcatcagca actatcctga caacaaactc
     8521 ggtaagaaaa ttaccctcaa gccttatcaa gcactggcaa ttcaagttaa ataaacgata
     8581 aaacatccga aaataacact ctacgtgtca attttaattt tcctaaaatt agaacccgtc
     8641 ttcacagctc agtaggttac ttgtgaaggc aagttcttgg tttaaggttt cttttaggaa
     8701 tatattttat acttagtttg cgcacgaaac agctatctgg ggggagatag ttgtttcttt
     8761 ttttatagtc atttgaatta actttgatac atcgtcactt tcggcttgcc atactctacg
     8821 aaagtgactt gtttcgcaag tcttattttc aacctagaac agcctctagg ctgttctagc
     8881 tacctgcacc tcagttcctt gtctgaaagt tcattcattc gactatatta ctacatataa
     8941 agcaataacc gtttcagata aggctgaaac ggttattttt gtaagtctaa ttaaaagata
     9001 catgcacatg atcgtagtgg ttttctgtaa cgctaccacg gtctggcatc agattccatg
     9061 tataggctgg accatagata ctgtcatacg gcgcatagaa gcgctgtttc caaatgatat
     9121 agttgatatt tttagaagct aagtttgcaa ctgcataagc cgccacttga tctcctagag
     9181 ctgagctctc aggcaccata aagtcaattg ccaatccctt accatggtcg ccagaatcac
     9241 caggacggta acctgagaaa gaagtaatac cgaaggcatt tgccacttct gcgcggaagg
     9301 ctgctacctg aggttgcaaa ccgacatcat aagcactagt acttgttgta gtggttgata
     9361 gggctgtcac ttgtggagtt tcaactacag gctgttccac aggtgcctca accacaggtt
     9421 gctctacaac ttcctctaca ggcgcttcta ctacaggctg ctctacaact tcctccacag
     9481 gtgcttctac tacaggttgc tctacaactt cctctacaga cgcttctgct acagacgctt
     9541 ctgctacagg ctgttctaca acttcctcta cgggtgcttc tactacaggc tgttccacaa
     9601 cttcctctac agacgcttct gctacaggtt gctccacaac ttcctctacg ggcgctgagt
     9661 cggtaactga tgaaatttga tccaagggaa caaccgtatc ctcaaccgtt acttgattgg
     9721 ttgttaggtc aacaattgcc tgaacggtct catttgtgtt tccctgaaca ggagcttcaa
     9781 tctcaacctg agtcacttga ttgttttggt caacagtcgt cgtcagtacc gtatcaggga
     9841 aaatcaagtt gacatctgca atttgattta ttttcgccag caagtccata tcaatattca
     9901 tagctaaggc gatggcagat aaggtatctc catactgaac agtataggtc atctttcctt
     9961 cttcttgtac aatttctggg ctaatttcag aaacagtacg tggagcccat tcgatggcta
    10021 aaacatttgt cgaaatcatc cctgtactaa agagggaaag agccaatgaa gatgtgtata
    10081 acatcttttt attgtttttc atgatgggtg tttttctctt gcgacgcata tgttgtggtt
    10141 tatttgttct cttacgtttc ataatcttat tccttcttta tttttgagat ggttctatct
    10201 tacctaaatt aaatctgaaa aacaagcctt caaccgttct tttaaataaa ctgtctccac
    10261 tatgtaatct taggactttt ctgtcacaca atcaatataa atgtcatctg actgtcatat
    10321 tattagataa tactcaatga aaatcaaaaa cagactcgga gacgcagatg cagatagaac
    10381 aaaagttcat caaatcaagt caacaacgtc tggattttcg aagactataa gtaaaaaata
    10441 agagtaaggc agtaatccaa caattggcta ctaactcact ctcatttcta ttttcggatt
    10501 agtcaaaaac ctacatttct tcaggataaa ccaagcccca ttctttacgg agttgggtca
    10561 tgatctccat gacatcttgg gtgtaggcta ggtgcatggt gttttcgatg cctgcgacag
    10621 ctttttccat atccgccact tcataagaaa gcgccttggc tgtatcgccc gcttcgatta
    10681 cctcttggct accgtcttca gtataggtaa tgactgcctt ctgaccacga ggatactcgt
    10741 aaagttcgat gtagcccttg tcgtaggcaa tagtaccccg ttttggctgt ttggcatgga
    10801 gggtcagggc gatggtcgcc atttcccccg catcattgct gagcaaaatc cctacctgct
    10861 cgtccacacc tgtcggagct aatttaacct gagataggac ttgatttggc ttttcagtca
    10921 taaaccaacg gacaaatgac agagcgtaga caccaatatc caagagggca ccgcctgcca
    10981 agtttttatt gaaaaagcgg ttggtcatgt catattcctt gtagctgcca aagttcatct
    11041 gaatcatctt caaatctcca agttttccac tggcaacaac ttcgctcaac tgacgataga
    11101 ttggcatgtg gaaaatggtc atagcttcta ccaggataac ctgattttct tctgccagtt
    11161 gaatggcttc tgctaattct tctgaattaa gcgtaataga cttttcacaa aggacatgct
    11221 ttccagcctt caaggccttt ctcaaataat tgatatgggt attgtgaggc gtagaaatat
    11281 agataatgtc tacttcagga tcctcaaaca cttcatcgat ttctcgataa actttctcga
    11341 tgccatattt ttgcgcaaat tccacaccct tgtcataggt acggttggct accgaataaa
    11401 gatttcctac catagcctgc aaggcctgaa ccaattcatt ggcaatgaca cctgttccca
    11461 gggtcgccca tttatacttc atttgcactt ccatagtgta cctcactttc ttctaccatt
    11521 atagtaaatt ccaccctttt ctgcacactc taactagcat ttgtgttaca ataagaacat
    11581 gaataccgtt gtactaaagg catctgccca gcaaaaacaa gccatgatgg ctcactatga
    11641 ccgctatcgg cagaccagca aaaatcctta catagaagca tttttcaaac tgaccggtgc
    11701 cagcctgtcc atctacactt ctggaaaagt cgtttttcag ggggaaatgg cagagcagga
    11761 agcagggctc tggggctatg agccagaaag ctctggacag acgactgtac ctggtcaaaa
    11821 cctgcccatg attggtaccg atgaggttgg aaacgggtct tattttggag gtttggcagt
    11881 cgttgctagt tttgtccgcc cagaagacca tgcctttctc aagtcgcttg gtgtggatga
    11941 ttccaagaaa atgacagacc aaaaaatctg ccaagtcgcc cctctcttaa aagagaaaat
    12001 cccccaccaa gctctgctat tgtcaccgaa aaaatacaac gaagtcatcg agcaaggcta
    12061 caatgcggtg tctgtcaagg tcgccctgca caatcaggct attttcctac tcttgcaaaa
    12121 gggtgtccag cctgagaaga ttgtcattga cgcttttact tccagtcaaa attatcaaaa
    12181 gtacttgaag aaggaagcca accaatttgc caaccccgtc actctggaag aaaaggctga
    12241 gggcaagtac ctggctgtgg cagtatcttc tattattgct agagctatgt ttttagaaaa
    12301 tcttgtccag cttggtcaac tagttgggat gaatctgccg tctggtgcgg gcagtaaatc
    12361 cgaccaagtc gcagcaagca tcttaaaaca atatggtatg gctggtctca accaaactgc
    12421 caaactccac tttgccaata cacaaaaagc acaaaaatta ttgaaatgag gaaaaaatat
    12481 gggagcaatc caaaaaacaa aacgctcacc actagtcgcc ttcttagctg aatggggtat
    12541 ctttttacta tttatggcag ctttttttgc cagtcgttac tttatctgga accctgtatc
    12601 tgtcgatggc cattctatgg acccaacgct tcaacaccaa gaaaaactca tcatgctaaa
    12661 aacaacctct atcgaccgct ttgatattgt tgtagccagt gagacagaca gcgacggcaa
    12721 ggaaaaactg attgtcaaac gtgtgattgg tatgccaggt gacaccattc gctacgaaaa
    12781 cgatgtcctc tatgttaacg accaaaaagt ggacgagcct tacttagatg aatatctagc
    12841 tgcattccag aaagataaac ttcaagaagt ctattcttac aacaaacaat tccaagctgt
    12901 ggcccaatct gctgaagctt tcacacaaga tgccaatggt tacgtagact ttaccgtgac
    12961 agtcccagaa ggacaatact acctgatggg agatgaccgc cttgtctcac tagatagtcg
    13021 tagtgtagga acattttccc gtgaaaacat caaaggagaa gttgtctttc gcatgtggcc
    13081 tctcaaccgt attgggactg ttgattaata caaacaagtt tggagcaatc cgttccaaac
    13141 ttctttttca ttattattgg aactaaactc tatgaacgaa gtgtatttta ccggtactat
    13201 tgaccggatt atttttgaaa atcctagtaa tttttataaa atccttctcc ttgaaatcga
    13261 ggaaactgac gctgactatg acgactacga aattattgtg accggaacga ttgccgatgt
    13321 cattgaagga gaagactatc gtttttatgg caatctggtc acccatccca agtatggtca
    13381 gcagctgcaa atttcgcgtt acgaacgcag caagcctacc tcagctggtc tggttaagta
    13441 tttctccagc gagcatttca agggaattgg acgcaaaaca gctgaaaaaa tcgttgagct
    13501 ctatggcgaa gataccattg ataagatttt agctgaacct gaaaaactaa cccaaatcac
    13561 aggcctatcc agcaagaaca tgcaggcatt tgtcgaaaaa ctccgcctca attatggaac
    13621 cgagttgatc ttggcaaaac tagccgagta tggcattccc aataaattgg cctttcaaat
    13681 ccaagaccag tataaggaaa aaacacttca gattattgaa gaaaatccct atcaactggt
    13741 cgaagatgtc caggggctcg gctttaccat tgccgatagg attgcggaaa acctgggaat
    13801 tgccagtgat tcaccccaac gttttcgggc tggcatgctc tttagtctca ttcatcgctc
    13861 catggaaaca ggcgatacct atgtggaggc cagagacttg ctggaagcaa ctcttgaact
    13921 gctggaaaaa tcccgccata cagagctgga ccccgctgct gttgccaaag aattgacggg
    13981 actgattgca gacgacaagg tacagcaaga aggcacaaag atttttgaca acagcctcta
    14041 ctttgccgaa catggtatcc ataaaaactt gacccgcctg atgggaaaaa atggtttcaa
    14101 acccttccca cgcacagacg ttgaggctgc tatagcagag ctggaaacta tgtcttccct
    14161 gacctacgat gacattcaaa aagaagccat cgtccaagcc attaccaatc cgcttttcat
    14221 tctgacaggc ggaccaggga ctggaaagac aaccgttatc aacggtatta tcgcggtcta
    14281 tgccatcttg cataagattg acctgactcg caaccgagaa gaatgcccag tcctcctagc
    14341 cgctccaact ggacgagcag ccagacggat gaatgaattg acaggtttgc cttccgccac
    14401 catccaccgc catctcggtc tggtagaagg acaggaagaa tcctaccgtg atgattattt
    14461 ggatgccgac tttatcatcg tggatgagtt ttctatggta gatacttggt tggcaaacca
    14521 gctcttccag aatatctcct cccagaccca agttctgatt gtcggcgatg ccgagcaatt
    14581 gccttctgtc agccccggtc aagtacttgc tgacctcttg aaaattgaca agctgcccag
    14641 cattactcta gaacgtatct atcgtcaatc cgatgattcg accattgtta ccctagccag
    14701 ccagattcga cagggtgctc tgcctagcga ttttcgtgag aaaaaggctg accgttccta
    14761 ttttgaagcc cagaatgaac aaattccagc cctgattgag cgcattgtcg gcgcagccat
    14821 caagtcaggt attcctgcaa acgaggtaca gatcctcgct cccatgtacc gtggggccgc
    14881 aggcatcgac caactcaaca ctatgaccca ggccctgctc aatccactgg aagagggaga
    14941 gttagaattc ctgcataacg agcaagcctt ccgccaaggc gaccgggtca ttcatttagt
    15001 caacgatgcc gaggccaatg tcttcaatgg tgatttaggc tacatcaccg acctccttcc
    15061 tgccaagtac accgactcca agcaggacga aattaccatc aacttcgacg gtagcgaggt
    15121 cacctatccc cgaaacgaat ggtacaagat taccctggcc tatgccatgt ccatccacaa
    15181 gtcccaaggc agcgagtttc aggtagtcat cctccccatc acccgcacca gccaccgtat
    15241 gctccagcgc aatctggtct acaccgccat cacccgctcc aagagcaagc tcatcctcct
    15301 gggcgaaatc tccgccttcg actacgccgt caaaaacgcc ggcaccgtcc gaaaaaccta
    15361 tctggtccca cgtttccaag gggaaatggc agagcaggaa agcaaggaaa gcctgattaa
    15421 aaaagacgaa agcaaaacag ctacaaccac gcaaccccag ccccctactc agccagacag
    15481 aaaagaacag gttaaagtag tcaaagaaaa caaccaacaa ctctcccttc ttgatcaaga
    15541 ccagccaaaa aaaacaagtc cccaacccac agaatacatt ttgactgtag acaacctact
    15601 aaccatcgac cccatgattg gcatagaaca ggcagatatt gaagaatttt ttaaagcata
    15661 aaaaccagca agtcagtaga tgaacaccgt tatattcttt tatttgacag cctctaaagc
    15721 tgctttataa tctgggtgtt cgttgacatt gtctaggtat tctacgtaga cgatctcatt
    15781 gtcggcatta aggacaaaga ccgcacgcgc cagcaaattc cactcccgca tgagcacacc
    15841 ataggatttt ccaaaggcat ggtcatagtc ccctaccatg gcgactgtct tgccctgttc
    15901 ttgtaagaac ttgacttggt tggccttgtc atctggcagg acctgactga ccacctgctc
    15961 aatgccgact tccttggcaa tagcctgggc agttttttca ttatctcctg tcagcatgac
    16021 aacctctagt tccatggttt gcagagcctg gacagcctga cggctggtct cttttacttt
    16081 atcagcaatg gcaatgactg ctagtacttc ttgctggctg gctaggaaaa caggcgtctt
    16141 agcttgatgg gcaaatgttt ccgcaactgc acgtcctttg gaaacatcaa ttccctgttc
    16201 ccgcatcaac cgttcatttc ccagatagat ggtctgctct gctattgtta ccgaaagacc
    16261 acgaccagaa agtgcctgaa aatcttggct ggcaagtaag ggaattgatt cctcttctgc
    16321 tgaacgtaag agggcttgag ccaatggatg ctcggagtat tgttcactgc ttgctgccag
    16381 ttggagaact tgctcacgat ttttagtaga caaaaggtgg atgtctgtca cttgtggctt
    16441 gccctcagta atggtcccag tcttgtcgaa gacaatggtg ttcacacctt gtagggtttt
    16501 aatagtttga ccagacttga acaaaagacc attttctgcc ccttttccag tcccaaccat
    16561 gatagcggtt ggtgtggcaa ggcccaaggc acacgggcag gcaatgacaa ggacggagat
    16621 ggtaatggaa agtgagaaca tctagggctc ttgtcccaag aagtaccaag caaggccaga
    16681 aaggacggct aatcccatta caattggaac aaagactgct gaaacgcggt cagccaactg
    16741 cgcaatcgga gcttttgacc cctgtgcttc ttccaccaaa cgaataattt gggacaatgt
    16801 ggtatctgct ccaacttttg tcgcttccat ttggaaagca ccatgttgat tgagagtcgc
    16861 accaacgact gtatccccga tgttttttta acaggcaaac tttcccctgt cagcatggat
    16921 tcatcaattg tcgattggcc ttctactatt atcccatcaa ctggaatctg ttcaccaggg
    16981 cggacttgaa ggacatctcc tactttgact tggtctaagg ggagggtcac ttcttgacct
    17041 tcacagagaa cgcgtgcagt tttaggggcg agattcatca gtttactgat agcttctgaa
    17101 gtacgctctt ttgataatgc ctccatgtat tttccaagag tgataagggt taaaataaca
    17161 gctgccgatt caaaatagag ctcaggatgt ccatgttggg tggctacttg gttggtagca
    17221 atcaaaatcg tcagtcataa tgatcccttg aaacaaggca gcacttgtcc caacggcaat
    17281 caaactatcc atattgggat gaccggcaaa cagggtttta aacccttttt gataaaatgc
    17341 acgaccaaca tataggactg gtaatacaag aatgagctga actagggcaa aaactagcgg
    17401 ctggtgtaag atagctggca atggcaatcc cccacctggt aacataggtc ccattgcaat
    17461 gtagagtagt ggaagcgtga aaatggttga ccagataaat ctgtgccata aatcattttt
    17521 gtgttgttct tttccgcttg gttgagcttc tgactcttgt gtttttggca gtaccgcttt
    17581 ataacctgct ttttcaacag ctgtcgatat ctgataagct cagatgggct ggatcatact
    17641 gaatgtttgt tttttccgtt gctagattca cactggcgtt ttgtattcca tctagcttat
    17701 tcaaggcttt ctcgactgtc atagcacagg aagcgcaggt cacgccttga atgggatagc
    17761 ttgcttgttt catagattac tccttttctg actacatttg taaacaaatt atacaacatc
    17821 cgactacatt tgtaaacaat tttgattaaa aaaatgagag aatcttctct cattaggtat
    17881 ctgatcaaaa gggtaataaa cttatatata ttctgtatgt gtttgaacat aggttaattt
    17941 gctaacgatt catttagata gatagaacaa tttgtatgga tttgcattag agttgatgga
    18001 aatccagtat ctggcttaga acaaactgtt ttacgtatga ccgcatgatg ccaatcacga
    18061 aaaactgcaa gtcgaatttt tttcgcttga aaaatctcgc tcattcctat tgttacacaa
    18121 taattaggca cttcttctac agctcctttt aataccgtca aaccattcat aacctttgtc
    18181 tcgacagaaa taggtaacac tcgcgaccct aattttaaaa attcttgaac tgttaatgtt
    18241 gaatccggtt cattgaatgc aatgtgccca tttaggccaa ttccaccaat acataagtcg
    18301 attttaccta attctttgat tttatttgaa atcgagtcta tatcaagagg atctggaaaa
    18361 attctttgtt cttctgacat aataagagtt ggatgtattt gactgtagac atttttctgc
    18421 ataaatcctc gaaaactaag agggtgtact acgtctatcc aatgcaaatc ttccaacaaa
    18481 tattcatcca tgttaataaa ccaaacatta gacaaatcaa tcttaaattg attcactaat
    18541 ctaacaaaat gcggatactg accaactggt ccaacaggac aaattatgac agttttctca
    18601 tttttttcgt tattttcctt tattaattct accatttcta gagcaagtga aataaaaatt
    18661 tcctcattat tcttttttac aatcaatgga attctactat gcttttctaa ttcactttta
    18721 ttaaaattgt aatattctat catgcattac ctcatgtttt cttgaaaact atgaatgatt
    18781 at
//
LOCUS       DAZQCJ010000031        18284 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000031 DAZQCJ010000000
VERSION     DAZQCJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18284
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.031"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..1220)
                     /locus_tag="ACHU66_000994"
     CDS             complement(<1..1220)
                     /locus_tag="ACHU66_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HFR3389287.1"
                     /translation="MEKQTFITLEDIDTSIAAENFNRLLDENKTYFLNGSWGSGKTTF
                     LESTESQSKKKFVTIDLWRNTDKRSLVEVAYASLHPVLYWGALLFSFLFVIISILMTN
                     TVNLGLSKFLHPIWMLPVGFISLVVAAVQLVAIKTEIVYSWLLANLCLKNKVLVIDDF
                     DRLTLSQQEEAYRLFSLLRGRLPIVFVGDIENIYSVKDNFLSKIIDRRVELPFVLQSE
                     QIWDDYFIKLEQRFATEISDSFKKRVKVEKRSLRDREHFNDYVNQEFFSRGKLGHVQV
                     EEQLLVIYSYLFYIEYYRKLLNNETIIENKSNSSDFSEIFTKGEEIPNLLSRLQRDNT
                     NKYPVSFKMNREGYFLYESPSNRKKAELDRILNGSEEQLSRELAYSNSGTDFYQYLSS
                     EYKYLKVDIKEKLLS"
     gene            complement(1363..2100)
                     /locus_tag="ACHU66_000995"
     CDS             complement(1363..2100)
                     /locus_tag="ACHU66_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389288.1"
                     /translation="MNETRFLELFNDLDKVLRKVCGVADGEYADVGSMLTKAKELTRH
                     NPVEANWDKLYVARQLRNLMVHEKRTGLKEVAQPSQELITVLEKVIAQYQEPITIADY
                     LQKTQKIKPVIFEGKDKLIKALEIVEQEHFSKFPIFSDKVYQGLVSDKGLTNWLAKAS
                     KETGSIREKLVEASLEDVLACEEDSIQVIVLPITAHLYQVINHFEGRKRTTILVSRQA
                     SGKLHSPEDLVGIVTAQDLAEIYGMVD"
     gene            complement(2102..3136)
                     /locus_tag="ACHU66_000996"
     CDS             complement(2102..3136)
                     /locus_tag="ACHU66_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFR3389289.1"
                     /translation="MKTLDYIVTLTNTPSPTGFTTDIMNYIKAEVESFGYAASKTAKG
                     GVLVTVPGENDQEHRMVTAHLDTLGAMVRAVKPDGRLKMDLVGGFGYPSIEGENCLIH
                     CAKNGKTFTGTILMHQTSVHVYRDASTAERNQTNMEIRLDEKVTNADETRTLGIEVGD
                     FISFDPRTVVTETGFIKSRHLDDKVSAAILLHLLKVYKEEGVTLPYTTHFYFSNNEEI
                     GYGANSSIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDGSGPYHYELRQHLVALAE
                     RDGIPYKLDIYPYYGSDASAAMRAGADVKHALLGAGIESSHSYERTHLDSVVATERMV
                     DAYLKSAMVD"
     gene            3201..4157
                     /gene="birA"
                     /locus_tag="ACHU66_000997"
     CDS             3201..4157
                     /gene="birA"
                     /locus_tag="ACHU66_000997"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR3389290.1"
                     /translation="MKTYQKIYLLLKEREDYVSGEDLAQELGISRTSIWKAIRQLETH
                     GLTIEAARNRGYRLAEGDLLLPELISQELQLPVHLNMDSDSTQLDAKQGIESGHTSPA
                     LYLAPYQNKAKGRFGRPFYASKSGGIYMSLRLSPNVPFLEFKPYTILAAAAVVKAIQS
                     LCDLDVQIKWVNDIYLGHKKVAGILTEAISSMENQRVTDVIIGVGINVRIDDFPKELQ
                     QSAGNLFEEQPPFTRNQLITAIWKAFLETDEKELIALYKEKSLVVGQQVSFVENQVEF
                     KGTAIAVTDTGNLVIQLDNGKAKIISSGEISLTSWSASPPNE"
     gene            complement(4123..4311)
                     /locus_tag="ACHU66_000998"
     CDS             complement(4123..4311)
                     /locus_tag="ACHU66_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HFR3389291.1"
                     /translation="MKLPQFLFLAITTILAVYFMNASILTGDFLIAGIYAFIAYRNLH
                     FAYKVTKFIRLVEKQTKK"
     gene            complement(4506..6173)
                     /gene="dnaX"
                     /locus_tag="ACHU66_000999"
     CDS             complement(4506..6173)
                     /gene="dnaX"
                     /locus_tag="ACHU66_000999"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR3389292.1"
                     /translation="MYQALYRKYRSQTFGEMVGQEVVATTLKQAIEQGKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVGGEPCNDCYICQAITEGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSLATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKSIKVTDIQEHLAAILTKEGLIFDEQALTIIARRAEGGMRDALS
                     ILDQALSLSQDQQVTLEIAEEITGSISLRALDDYVASLRQGDSVTALQHLQTLFDQGK
                     SMSRFATDLLHYLRDILIVQTGGEDTHLTTGFSENLALEQARIFTMIEMVTRGLADIK
                     SSPQPKIYAEMMTIRLAEDSSPSGAMAELPADLLGQLASLQQQVADLQKQLGQLSSQP
                     SAVKPVSRKPQAPKKYRLDTSKVHAILQEAMENPTLARENLTRLQNAWGEIIESLSGA
                     DRALLVGSQPVAANENHAILAFDSPLNAEQTMKRDNLNTMFGNILSKAAGFSPQILAI
                     AQEDWVSIRAEFSAKAKGQKTQEIEKEASPVPEEFGFLADTVEIRND"
     gene            complement(6173..6670)
                     /locus_tag="ACHU66_001000"
     CDS             complement(6173..6670)
                     /locus_tag="ACHU66_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFR3389293.1"
                     /translation="MTKQEKISNYQLLLAQLEALLEGETNALANLSNASALLNQALPN
                     SVFTGFYLYDGSELILGPFQGGVSCVHIALGKGVCGEAAAKRQTILVDDVRLHDNYIS
                     CDATALSEIVVPMVKNDQLLGVLDLDSRLVADYDTIDQDYLEKFVALLVEKTDWNFAM
                     FGEKR"
     gene            complement(6735..7004)
                     /locus_tag="ACHU66_001001"
     CDS             complement(6735..7004)
                     /locus_tag="ACHU66_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFR3389294.1"
                     /translation="MSIKQMVSMALVVWNLVVFITYGMDKGKARKNAYRISEKTLLLM
                     SYFGGGLGAWAGGTHFRHKTQKKYFQLAWAIGVLIDAVIMYWMWK"
     gene            complement(7092..7630)
                     /locus_tag="ACHU66_001002"
                     /pseudo
     CDS             complement(7092..7630)
                     /locus_tag="ACHU66_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
     gene            complement(7632..8261)
                     /gene="udk"
                     /locus_tag="ACHU66_001003"
     CDS             complement(7632..8261)
                     /gene="udk"
                     /locus_tag="ACHU66_001003"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR3389295.1"
                     /translation="MTQKPIIIGVTGGSGGGKTSVSRAILDNFPNARISMIEHDSYYK
                     NQSHLTFEERILTNYDHPLAFDTDLMIYHISELLAGRSVDIPIYDYTQHTRSEKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDIRIIRRIKRDMEERGRSLDSIIEQ
                     YTSVVKPMYHQFIEPTKRYADIVIPEGVSNVVAIDLINTKVESILRERG"
     gene            8344..9330
                     /locus_tag="ACHU66_001004"
     CDS             8344..9330
                     /locus_tag="ACHU66_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR3389296.1"
                     /translation="MLNFGIVGTSDISHRFIEAAHLSGHYQLQAVFSRKLETAAAFSE
                     QYEGVDLYTEWVKFLSAPIDVVYIASPNALHFEQAKAVLAAGKHAIVEKPMVSTPQEL
                     EQLRRVAQENNVFLFEAARNYHEQAQAIIREFLTDKTILGGSFGYAKYSSKMPELLAG
                     QMPNIFSTDFSGGALMDLGVYTLYAAIGILGTPRTARYVAQQLPSSIDLNGSGQLIYD
                     NYIVHIQAGKNITSNLASEIYTNDGTLTLNACQYISSAIFTKHDGEQIVLPIQAAQHP
                     MLEEALEIAQAIKQQNRKLVDQWLAVAESVHQTLYTMRQDAGIIFKADNNEI"
     gene            9320..10402
                     /locus_tag="ACHU66_001005"
     CDS             9320..10402
                     /locus_tag="ACHU66_001005"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR3389297.1"
                     /translation="MKSKFPSSWTDQLTLLGFEDFTPIQVQAFEPIANGKSLLAISPT
                     GTGKTLAYLWPSLLALTPKKAQQLLILAPNTELAGQIFDVCKTWSETIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNINTIVLDEFDQLFSDSQYQFV
                     EKIINYVPRDHQLIYMSATAKFDRQKIAEDIESIDLSEQKLDNIQHCYMMVDKRERLE
                     TLRKFANIPDFRALAFFNSLSDLGASEDKLLYNGVNAVSLASDVNVKFRKVIIERFKN
                     HELNLLLATDMVARGIDIDNLECVLNFEVPFDQEAYTHRSGRTGRMGKEGLVITLVSS
                     PSELKQLKKYASVQEVILKNQELYKI"
     gene            complement(10469..11866)
                     /locus_tag="ACHU66_001006"
     CDS             complement(10469..11866)
                     /locus_tag="ACHU66_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR3389298.1"
                     /translation="MKKVSFWLGINIALLGIMVSLAVWLFAGLQERQVSQFIEEKQQT
                     ILAKGKGKIQEGNIDTTHVVAALPTDDAGHVLGPVESRMISYVQRRFGHKKPAGKIQN
                     LVFVSSIEGKTNFKNVTAREIQAEQYKVDNLQIKKQDKLPSERVLLTQDNKLFTLEDL
                     LPNLSSTASIIVGHLREALLAQGMKETDVEAIVKKFETLDLNAISFSYGDSQLTLQLP
                     DGYGINQLVLPISDLYPVVKSDYLVDADKVGYDEYMAAQVVDKKHLRQVALTFDDGPN
                     PNTTPVVLDLLKKYNAKATFFVVGKAVAGNEAILRRMVAEGHVIANHTWNHPNLVTIS
                     GEQVQREIQDTQAAITEATGIVPTMVRPPYGSVNQAVVNQIGLPSIYWSVDSKDWKSR
                     NPQAILKEIKEQTCPGSIILMHDIHQSTVDSLESVLQYLTGEGYNMVTVTDLLASPLN
                     PQLIYYSQELSGPAQ"
     gene            complement(12156..12539)
                     /locus_tag="ACHU66_001007"
     CDS             complement(12156..12539)
                     /locus_tag="ACHU66_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR3389299.1"
                     /translation="MASKKMLHACLRVENLEASQKFYEEAFGFKETRRKDYPEHKFTL
                     VYLALEGDGFEIELTYNYDHGPYIVGDGFSHLALSSDDLEGDNAKHKELGYPTTDIKS
                     LPGSPGRYYFVTAPDGYRVEVIRAD"
     gene            complement(12645..13337)
                     /locus_tag="ACHU66_001008"
     CDS             complement(12645..13337)
                     /locus_tag="ACHU66_001008"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HFR3389300.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDLAFTSVLKRAIKTTNLALEAADQLWVPVEKSWRLNERHYGGLTGLNKAEAA
                     AEFGDEQVHIWRRSYDTLPPEMAKDHEHSAHTDRRYAHLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKQLSDDEIMDVEIPNFPPLVFELDE
                     NLNIVKEYYLEA"
     gene            13563..14747
                     /locus_tag="ACHU66_001009"
     CDS             13563..14747
                     /locus_tag="ACHU66_001009"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR3389301.1"
                     /translation="MKKVAIVSAYRSAIGSFGGSLKDIEIADLGAQVLETALANKNIP
                     ADSVDEVIFGNVLSAGQGQNIARQIAIRAGIPQTASAYAVNKVCGSGLKSVLLAAQSI
                     MLGDNDVVVAGGIEIMSQAPYLSKTSRFGSKFGHISLEDSMLTDGLTDAFNDYHMGIT
                     AENVAERYHVSRAEQDAFTYSSQEKAAKAIAEGRFVDEIVPIRLKNRKGETIFATDEY
                     PRLTPIEKLATLRPSFKKEGTVTAANASGINDGCAVLVLMSDEKASELNIQPLAYIEA
                     YATSGLDPALMGLGPITASQKALQKLNKTVEDIDLFELNEAFAAQSIPVVKQLGIDPA
                     KVNVNGGAIALGHPIGASGSRILVTLIHELIKQEKELGLCSLCIGGGQGISLIVSNAQ
                     TN"
     gene            14776..15930
                     /locus_tag="ACHU66_001010"
     CDS             14776..15930
                     /locus_tag="ACHU66_001010"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFR3389302.1"
                     /translation="MNIGIDKIGFAAPDYVLDLADLAQARNVDPNKFKIGLLQSEMAV
                     APVTQDIISLGAKAAEAILTEEDKQTIDMVIVGTESSVDQSKAAAVTIHGLLGIQPFA
                     RSIEMKEACYGATAGLSLAKSHIAQFPESKVLVIASDIAKYGVASGGEPTQGAGAVAM
                     LVTANPQILVLNNDNVCQTRDIYDFWRPNYDKYPRVDGKFSTEQYTDCLTTTFDYYQQ
                     KTGKTLNDFAAMCLHIPFSKQGLKGLQAIAQDEETLSRLTERFQEAIVYNKVVGNIYT
                     GSIFLSLLSLLENSRALETGDQILFYSYGSGAVCEIFSGQLVEDYRDHLQENRLEQLN
                     QRTKLSVKEYEQVFFEEITLDETGSSLNLPEDQSPFALIKVDNHKRIYRK"
     gene            16191..17450
                     /locus_tag="ACHU66_001011"
     CDS             16191..17450
                     /locus_tag="ACHU66_001011"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFR3389303.1"
                     /translation="MSTFSGFYKKSRQERIDILRQNRSLSEDSLDILYKDENLPEAIA
                     GKMAENHLGTFSLPFSVLPELLVDGQTYSVPMVTEEPSVVAAASFGAKIIAKSGGFTT
                     TIHNRIMIGQVALYDISDHSRATQAILDHKESILETANQAHPSIVKRGGGARELTVES
                     KDEFLIVYLQVDVQEAMGANILNNMLEAIKDDLEELSKGQALMGILSNYATESLVTAQ
                     CHIAISSLATSSAIAQETAQKIALASKLAQVDPYRAATHNKGIFNGIDAVVIATGNDW
                     RAVEAGAHAYASRDGQYKGLSTWSIDGEHLVGSITLPLPIASVGGSIGLNPKVAVAFD
                     LLQQPKARQLASIIASVGLCQNFAALRALVTSGIQAGHMKLHAKSLALLAGAEEHEVD
                     QLAQLLRKEKHSNLETAQNLLAKMREH"
     gene            complement(17472..17939)
                     /locus_tag="ACHU66_001012"
     CDS             complement(17472..17939)
                     /locus_tag="ACHU66_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFR3389304.1"
                     /translation="MTIRKARQSDISVLNELLQDILQVHHQVRPDIFKSAGQKFSEAE
                     LVALLENPDKPIFVFELEGQVVGHLFCELSTTTGDVLEPVKTLFIDDLCVANSARGQK
                     IGEQLYEFALSYAKEQGCHNLTLDVWADNAGAVRFYERQGMKPQKFRMEQIIN"
     gene            complement(18059..>18284)
                     /locus_tag="ACHU66_001013"
     CDS             complement(18059..>18284)
                     /locus_tag="ACHU66_001013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389305.1"
                     /translation="FFLFKGQIRGKTLYSMKIKSSLGNEAEDRTGVHQGKLTTDNFDF
                     RRVLVVFYTTHSRFKTTKNQPWPADFLGDL"
BASE COUNT         5360 a         4159 c         3423 g         5342 t
ORIGIN      
        1 gatagtaact tctcttttat atcaactttt agatacttgt attcagatga aagatactga
       61 taaaaatctg taccactatt tgagtaagct aattccctag acaattgttc ttctgatcca
      121 tttaatatcc tatctagttc tgcctttttc ctatttgaag gcgattcata aagaaaatat
      181 ccctctctat tcatcttaaa acttactgga tatttgtttg tgttatcacg ttgtaggcga
      241 gacagaaggt tgggaatttc ctctccttta gtaaatatct cactaaaatc tgacgaattg
      301 cttttatttt caatgatcgt ttcattattg agtaacttcc tatagtattc aatatagaaa
      361 agataggaat aaatgactaa taattgttcc tctacctgaa catgcccaag ttttcctctg
      421 gaaaaaaatt cttggttaac atagtcattg aaatgctctc tatcccttag acttctcttt
      481 tctaccttta ctcttttttt gaaactatca gaaatttcag tggcaaatct ttgttctaac
      541 tttataaaat aatcatccca aatctgttca gattgaagga caaacggcaa ctctaccctc
      601 ctatcaataa tttttgatag aaaattatct tttacactat aaatattctc gatatcacca
      661 acaaagacta taggcaacct ccctcttaac aaggaaaata atctatatgc ttcctcctgt
      721 tgtgagagcg ttaaacggtc aaagtcgtca atcactagaa ctttattttt taagcacaaa
      781 ttagccaata accaagaata aactatttct gtctttatcg ctactaattg caccgccgca
      841 actactaggg aaataaaccc aacagggagc atccatatgg ggtgtaaaaa tttacttaat
      901 cctagattta ctgtattggt cattaaaata gagatgatta caaaaagaaa actgaatagt
      961 aatgctcccc agtataaaac aggatgtaaa ctggcataag caacttcaac aagacttctt
     1021 ttgtcagtat ttctccataa atcaatagta acgaacttct tttttgattg cgactctgtt
     1081 gattctaaaa acgttgtttt tcctgatccc cacgaaccat tcaaaaagta tgttttattt
     1141 tcatccaaaa gtctattaaa attttctgct gcaatagagg tatcaatatc ttctaaagtg
     1201 ataaaagttt gtttttccat attttttcct aaaaaatatt tatattgtga caatgtagaa
     1261 ttgggactcc taaatatctt ttatttacga gtttcacaac atattcaagt ctttttcaac
     1321 acagtaatac accttgataa tggattaaca agaactattg cttcaatcca ccatcccata
     1381 aatctctgcc aagtcctggg ctgtgacaat accaacaaga tcctctggcg aatgaagctt
     1441 cccactcgcc tgacgactga ctaggatagt tgtgcgctta cgcccctcaa aatgattaat
     1501 tacctgataa agatgggctg ttattggtag aacaattacc tgaatactat cttcctcaca
     1561 agctagtacg tcttcaagac tagcctccac caatttttca cgtatagagc ccgtttcctt
     1621 gcttgccttg gccaaccaat ttgtcaatcc cttgtcagaa accaggcctt gataaacctt
     1681 gtcagaaaaa ataggaaatt tagaaaaatg ctcttgctcc acaatttcta aagcctttat
     1741 caatttgtcc ttgccctcaa aaataaccgg cttaattttc tgggtctttt gcaaatagtc
     1801 ggcaatagta atcggttctt ggtactgggc tatcactttt tccaaaacag tgatcaattc
     1861 ctgcgaaggt tgggctactt ccttcaaacc tgttcgtttc tcatgaacca tgagattacg
     1921 caactgacga gccacataga gtttatccca gttcgcctca acgggattat gtcgcgtcaa
     1981 ttcctttgcc ttggtcaaca tggaaccaac gtccgcatat tccccatccg caacaccgca
     2041 caccttacgc aagaccttat ccaagtcatt gaataactct aaaaaacggg tttcattcat
     2101 cttaatctac cattgctgat ttcaaatagg catctaccat ccgctcagtc gcaactacag
     2161 aatccaaatg ggtccgctcg taggaatggc tagactcaat gccagcacca agcagtgcat
     2221 gcttgacatc tgcacctgct cgcatagctg ccgaagcatc ggaaccatag taaggataaa
     2281 tatccagctt ataaggaatt ccatctcttt cagccaaggc taccaagtgc tgacgaagtt
     2341 cataatggta gggacctgag ccgtccttga cacagataga aactgtatat tcgtcagttt
     2401 gctggtcatc tcccattgcc cccatatcga ctgccaaata ttcaaccacc tgagcaggaa
     2461 tgctggagtt ggcaccgtag ccaatctctt cgttgtttga aaaatagaaa tgtgtcgtat
     2521 aaggcagggt cactccttct tccttataga ccttcaaaag atgaagcaag atagctgcgg
     2581 ataccttatc atccaagtga cgactcttga taaagccagt ttctgtcacg acggtacgag
     2641 gatcaaagga gataaaatct ccgacttcaa tacccaaggt ccgcgtctcg tccgcattgg
     2701 tcactttttc gtccaaacga atttccatgt tggtctgatt gcgctcagcg gtactggcat
     2761 cccgatagac atggacagag gtctggtgca tgagaatagt acctgtgaaa gtcttgccat
     2821 ttttagcgca atgaattaag cagttttctc cctcaataga agggtatcca aatcctccca
     2881 ccaaatccat tttaagtcgg ccatctggtt tcactgcacg aaccatggcc cccagtgtgt
     2941 ctaggtgagc agtcaccatg cggtgttcct ggtcattttc ccccggaact gtcaccaaaa
     3001 caccaccttt agcggtctta ctagcagcat agccgaaact ttcaacctct gccttgatgt
     3061 aattcataat atccgtcgta aaaccagttg gcgacggagt gttggtcaat gtaacaatgt
     3121 aatcaagtgt cttcatgaaa acctccttat ttgaaactat tatatcacgt tttaggtgaa
     3181 ttttttgcta taatgaaggt atgaaaacct atcagaaaat atatctcctt ttaaaagaga
     3241 gagaggatta tgtcagcgga gaagatttgg cacaggaatt gggcatttca cggacatcca
     3301 tttggaaggc cattcgccag ttagaaacgc atggtttaac tattgaggct gcccgtaatc
     3361 gtggctacag attagccgaa ggcgatttac tcctacccga attaatctct caggagcttc
     3421 aactacctgt ccatctgaat atggatagcg attccaccca actagatgcc aaacaaggta
     3481 ttgaatcagg tcatactagt cctgccctat atttagctcc ctatcagaat aaagccaagg
     3541 ggcgatttgg gagacctttt tatgcatcca agtcaggtgg catttatatg tcgcttcgtc
     3601 tctctcccaa tgttcccttt ctagaattta agccctacac cattttagct gcagctgctg
     3661 ttgtgaaggc cattcaatct ctttgcgact tggacgtcca aatcaagtgg gtcaatgaca
     3721 tctatctcgg acacaaaaag gtcgctggca tcctcactga ggctatttct tctatggaaa
     3781 accaacgggt aaccgatgtc attatcggtg tcggcatcaa tgtccgcata gacgatttcc
     3841 ctaaggaatt acaacaatct gctggaaacc ttttcgagga acaaccacca tttacccgca
     3901 atcaactcat tactgccatc tggaaagcct tcttagaaac cgatgagaag gaactaattg
     3961 ccctttacaa agaaaaatca cttgtcgttg gccaacaagt gagctttgta gaaaatcaag
     4021 ttgaatttaa ggggactgcc atcgctgtta ctgatacagg aaacctggtt atccagttag
     4081 ataacggcaa agcaaaaatt atctccagcg gagaaatcag ccttacttct tggtctgctt
     4141 ctccaccaaa cgaataaatt tagtcacctt gtaagcaaaa tgaagattcc gataggcgat
     4201 aaaggcgtag atacctgcaa tcaagaaatc tcctgtcaaa atcgatgcat tcataaaata
     4261 aacagctaaa atagttgtaa tggctaaaaa aagaaattgt ggaagtttca taaaatagat
     4321 tataccaaaa attcagtcaa ctggctgaat ttttatatgc tttcttgata ggtttcgatt
     4381 cttttctaaa caagaaaaca ccaatccaat tggtgctttc ttaaggagat atgaaaaata
     4441 tttaggattg aatatagtat aaaatatcta tcaaaactat tcatcagtct taggactgat
     4501 attttctaat catttctaat ctcaactgta tctgcgagaa agccaaattc ttctgggact
     4561 ggactggctt ctttttcgat ttcctgtgtc ttttgccctt ttgctttcgc cgaaaattca
     4621 gcacgaatac ttacccaatc ttcctgagca atagctaaaa tctgtggcga aaaacctgcg
     4681 gccttgctga gaatatttcc aaacatggta ttgagattgt cacgcttcat cgtctgttca
     4741 gcattaagcg gagaatcaaa ggctagaatc gcatgatttt cattggcagc aactggttga
     4801 gaaccaacaa gcaaggcacg atctgctcct gacagactct cgataatttc tccccaagca
     4861 ttctgcaagc gagtcaaatt ttcacgcgcc aaggttggat tttccatagc ttcctgcaaa
     4921 attgcatgaa ccttgctggt atccaatcgg tacttcttcg gagcctgtgg cttgcgtgaa
     4981 actggcttaa cagcacttgg ttgactagat aattgaccta gttgtttctg taaatccgca
     5041 acttgttgtt gcagactggc tagttgcccc agtaaatcag ctggcagctc tgccatggcc
     5101 cccgaaggag aactatcttc agccaaacga atggtcatca tttcagcgta aatcttcggt
     5161 tgaggactgg acttgatgtc cgccaaaccc ctggtcacca tctctatcat ggtaaaaatc
     5221 cgtgcctgct caagagctag attttctgaa aaacctgttg ttaaatgcgt atcctctcca
     5281 cccgtctgca cgataaggat atctctcaaa tagtgcaaaa gatctgtcgc aaaacggctc
     5341 atactctttc cttggtcaaa caaggtttgt agatgctgga gagctgtgac actatcccct
     5401 tgacgcagac ttgccacata gtcatccaag gcacgcaagc tgatagaacc cgtaatttcc
     5461 tcagcaattt ccaaggtcac ttgctggtcc tgactaaggc tgagagcctg atccaaaatg
     5521 gatagggcat cccgcatccc cccttctgcc cggcgcgcaa tgattgtcag agcctgctcg
     5581 tcaaagatta gcccttcctt ggtcaaaatt gcagccaagt gctcctgaat gtccgtcacc
     5641 ttgatggact taaattcaaa tcgttgcacc cttgacaaaa tagtagcagg aatcttgtgc
     5701 aattccgtcg ttgctagaat aaagaccaca ttttcagtcg gctcttccaa ggtcttcaag
     5761 agggcattga aggctccagt agacagcatg tggacctcgt cgatgatata gaccttatag
     5821 gtcgcaagac taggtgcata ggtcgattta tcacgaatat cacgaatctc atccacacca
     5881 ttgttggaag ccgcatcaat ctcaatgaca tcttccaggc taccttccgt aatagcctga
     5941 cagatataac aatcgttaca aggctcgcct ccaacctgat tgggacagtt catggccttg
     6001 gcaaaaatct tagctgccga tgtcttaccc gtaccacgag gacctgaaaa gagataggca
     6061 tgactgattt ttccctgttc aatggcctgt ttgagcgtgg tcgcaaccac ctcctgcccc
     6121 accatctccc caaaggtctg gcttcggtac ttcctatata aagcttggta cattagcgtt
     6181 tttctccaaa catagcaaaa ttccagtccg tcttctcaac caagagagca acgaactttt
     6241 ctagatagtc ttggtcaatc gtatcataat cagctaccaa tctcgaatcc aagtccaaaa
     6301 cgcccagcaa ctggtcgttt ttcaccattg gcacaacgat ttctgatagg gctgtcgcat
     6361 cgcaggaaat atagttatca tgtagacgaa catcgtctac gagaatagtc tggcgcttag
     6421 ctgcagcctc accgcagaca ccctttccaa gtgcaatatg aacacaggaa acgccaccct
     6481 gaaaaggccc caaaatcaat tcactaccat catacaagta aaatcctgta aagacagagt
     6541 ttggtagagc ttgattgagc aaagcagaag catttgacag attggctaaa gcattggttt
     6601 cgccctctaa aagcgcctcc aattgggcca ataatagttg atagtttgaa attttttctt
     6661 gttttgtcat aggaacatta tagcatagtt aagccaactt ttatagtccg agcgataaag
     6721 aaaaaggctt acgcctattt ccacatccaa tacataatca ctgcatcaat caatacccct
     6781 atagcccagg ctagttgaaa atactttttc tgtgtcttat gccgaaaatg ggttccacct
     6841 gcccaggcac ccagcccgcc accaaaatag gacatgagta ataaggtctt ttctgaaatc
     6901 cgataggcgt tctttcttgc cttcccttta tccataccat aggtaatgaa gacaactaaa
     6961 ttccaaacta ctagcgccat actaaccatc tgtttgattg acatacattc ctccaattaa
     7021 gtcacatttc ttcttactct aacataatcc aaaacaggac acaaggaaaa acgattcgga
     7081 aatccgaatc gttacatttc cgccagtgta tcctctaacc atttgagctg ggcacggttg
     7141 cgttcaatag cccgtgtcaa aatcaaatag tgtccataat tgtcttggat actttccttg
     7201 gttgagaaca gctcaacttt acgactttct aggtgtttta agtgcttggt gactagttca
     7261 atttgactct gcaacaaacc aggaatccgt gggtcatcct tttcacggat aaaaaacatc
     7321 ttgattgaaa acaagtctct ttgctgaggc gtttcttcat ttggaatgga tagccattcg
     7381 tctaaaatct gacgaccagt agccgtcatc gcatactgct tttccttttc atttccaggt
     7441 actgcatgac aagtaatcaa ttcttccttg gtcatacgtt ttaattctgg atagacttgg
     7501 ctatggcaac ctgccaaaat tcgcctaaat cacgttgaac atagtcggta atctgcttgc
     7561 cagttaccat ttgaccgcta gcacccataa tccctaatat gatatgaggt aatattcttt
     7621 gcttcggcat cttaaccacg ctctcttaat atactctcca ccttggtgtt aatcaagtcg
     7681 atagctacaa cgttgctaac gccttcagga atgacgatat ctgcatagcg cttggttggc
     7741 tcgataaact ggtgatacat tggtttcacc accgaggtgt actgctcaat gatactatct
     7801 aagctacggc cacgctcttc catgtctctc ttaatgcggc gaatgatacg aatatcgtca
     7861 tcagtatcga cgaatatctt aatatccatt aaatcccgca accgtttgtc ttccaatact
     7921 aaaatacctt ccacaataaa tacatcttgt ggttcctgac gataggtctt ctctgaacgt
     7981 gtgtgctgag tgtaatcata aatcggaata tccactgaac gtcctgctaa tagctcactg
     8041 atatgataaa tcatcaaatc cgtatcaaaa gccagtggat ggtcataatt ggttagtatc
     8101 cgttcttcaa aggtcaggtg ggactgattc ttataataag aatcgtgttc aatcatagat
     8161 atacgagcat ttgggaaatt atccagaatg gcacgtgaca cacttgtctt gccaccccca
     8221 gatccgccgg ttacgccaat aataatgggt ttctgagtca tcatcatctc cttctatttt
     8281 caatactatt ttaccatgaa acatggtata atgaaaagga taacacaaaa gaaatgagaa
     8341 tttatgctta attttggtat tgttggcacc tcagacatct ctcatcgttt tatagaggcg
     8401 gcccatttaa gtggacatta tcagctacag gctgttttct cccgtaaact agaaactgcc
     8461 gctgcctttt cagagcagta tgaaggagtc gatttataca cagaatgggt aaaatttctt
     8521 tctgccccca ttgatgttgt ctatattgcc agtccaaatg ccctgcactt tgaacaggca
     8581 aaagctgttc tagctgctgg taaacatgct attgttgaga agcccatggt ttctacacct
     8641 caggaattag aacaattgcg tcgcgttgcc caagaaaata atgttttcct ctttgaggct
     8701 gctcgaaact atcacgaaca agctcaagcc attatccgtg aatttttgac ggataaaacg
     8761 attttaggtg gttcatttgg gtatgccaaa tattcttcta agatgcctga attgttagct
     8821 gggcaaatgc caaatatctt ttcaactgat ttttccggtg gtgctctcat ggatttaggt
     8881 gtctatacgc tctacgccgc tatcggcatt ttgggaactc ccagaacggc ccgctatgta
     8941 gcacaacagc taccttctag tattgactta aatggttcgg gacaactcat ttatgacaac
     9001 tatatcgtcc acattcaagc aggaaaaaat atcacgagca atctagcgag cgaaatctat
     9061 acaaatgatg gaacactaac actcaacgca tgccagtata tcagctctgc tattttcaca
     9121 aaacatgacg gagagcaaat tgttttacca atccaagcgg cccagcatcc tatgctcgaa
     9181 gaagcgctag aaattgcaca ggcaatcaag caacaaaacc gcaagcttgt ggaccagtgg
     9241 ttggctgtgg cagaaagcgt ccatcaaaca ctttacacca tgcgtcaaga cgcaggaata
     9301 atctttaagg cggataacaa tgaaatctaa atttccctct agctggacgg accagcttac
     9361 cctacttggc tttgaggact ttactcctat ccaagtccag gccttcgagc ctattgcaaa
     9421 cggcaagtcc cttctagcta tcagcccaac aggcacagga aaaacccttg cctacctctg
     9481 gcctagccta ttagccctaa ctcctaaaaa agcccaacaa ttgctcatcc tcgcacccaa
     9541 tacagagctg gccggccaaa tatttgatgt ttgtaagact tggtcggaga caattgggtt
     9601 gactgctcaa ctattcttgt ctggttccag tcaaaaacgc cagattgagc gactaaaaaa
     9661 agggccagaa atcctgattg gaacacctgg tcgtattttc gagttgatta aattgaaaaa
     9721 aatcaagatg atgaatatca acaccatcgt cctagatgag tttgatcagc tattctccga
     9781 ttctcaatat cagtttgttg aaaagattat caactatgta cctcgtgacc accagttaat
     9841 ctacatgagt gcaacagcta aatttgaccg tcaaaaaatt gctgaagaca ttgaaagcat
     9901 tgatttatct gagcagaaat tggataatat ccagcattgc tatatgatgg tagataagcg
     9961 tgaacgtttg gaaactctac gtaagtttgc caatatccct gattttcgtg ctcttgcatt
    10021 ttttaacagc ctatcagacc ttggtgcaag cgaagataaa ctactctata atggtgtaaa
    10081 tgcagtttca ttggcttctg atgtcaacgt aaaattccgc aaggtcatca tcgaacgttt
    10141 caagaatcac gaactcaatc ttctacttgc tacagatatg gtcgcacgcg gtatcgatat
    10201 tgataactta gaatgtgtcc tcaactttga agttcctttt gaccaagaag cctacactca
    10261 tcgttctgga cgtactggac gcatgggcaa ggaaggtctt gtcatcaccc tagtatctag
    10321 tcctagcgaa ttaaaacaat tgaaaaagta tgcctccgtc caagaagtca ttctcaagaa
    10381 tcaagagctc tacaaaatat aatgaacaat atacaaaagg cttagaatca tgttagtaga
    10441 aacactggtt ctaagccttt tatcattctt actgggctgg gccagataat tcttgtgaat
    10501 aatagattaa ttgaggattg agaggactgg cgagtaagtc tgttacagta accatattgt
    10561 agccctcacc tgttaaatat tgcaatacag actcgaggct atcaactgtt gactgatgaa
    10621 tatcatgcat taggataata cttcctggac aagtctgttc ttttatttct ttcaaaatgg
    10681 cttggggatt gcgactcttc caatctttag aatcgaccga ccaataaatc gatggaagtc
    10741 ctatctgatt gacaacagcc tggttaaccg aaccataagg agggcgaacc attgttggta
    10801 caatacctgt agcttcagta atagctgcct gtgtatcttg aatttctctc tgtacttgct
    10861 ccccagaaat agtgactaga ttgggatgat tccacgtatg gttggcgatg acgtgccctt
    10921 ctgctaccat tctacggaga atagcttcat tcccagctac agccttacca acaacaaaga
    10981 aagttgcttt ggcattatat tttttcagta aatctaaaac taccggtgtt gtgttcggat
    11041 ttggtccatc atcaaaggtt agagcgacct gtctcaaatg tttcttatcc acaacttgtg
    11101 cagccatata ctcatcatag ccaaccttat cagcatctac tagataatca ctcttcacta
    11161 ctgggtacaa atctgaaatt ggtagaacca actggttgat gccgtaacca tctggtaatt
    11221 gtaaggttaa ttggctgtct ccataactaa aggaaatagc atttaagtct agtgtttcaa
    11281 attttttgac aatcgcttct acatcagtct ctttcattcc ttgagcaagt agggcttctc
    11341 ttagatgacc aacaataata cttgctgtag aggataaatt aggtaataaa tcttccaggg
    11401 taaacaattt attgtcttgc gtcaacaaca cacgctcaga cgggagttta tcctgctttt
    11461 taatttgcaa gttgtctact ttatactgct ccgcttgaat ttcacgagct gttacatttt
    11521 taaaatttgt ttttccctct attgatgaaa caaagaccag gttttgaatt tttcctgctg
    11581 gttttttatg accaaagcgt ctttgaacat aggaaatcat tcgactttct acaggtccta
    11641 agacatgacc tgcatcatct gtaggtaagg cagccacaac atgggtcgta tcaatatttc
    11701 cctcttgaat tttgcccttg cctttagcga ggatggtttg ttgtttttcc tcgataaact
    11761 gactaacctg tctttcttga agtcctgcaa ataaccatac agccaaactt accattatgc
    11821 cgagcaaggc gatgttgatt cccaaccaaa aactaacttt tttcatatcc agcctcccat
    11881 tcaaaacata tataaactat gctaaatcat ttaaaggctc ttgtcaagac agttctataa
    11941 aaactcataa aaagattagg taatactctt cgaaaatcaa aattatccgt tgtcaacttg
    12001 ccttgatgaa ctccagttct atcttcggct tcgttttcta ggctactttt gattttcatt
    12061 aagtataaat aattgatttg cttcctaatc cctcccttct gccattttct gatttttttg
    12121 aaaagaaaaa gactgcctag gcaatctttt ctaaattaat ctgcacgaat gacttccaca
    12181 cggtagccat ctggagctgt cacaaaataa taacgacctg gacttcctgg caagctcttg
    12241 atgtctgtcg ttggatagcc cagctcctta tgcttagcat tgtctccttc taaatcatct
    12301 gaagaaagag ctagatgcga gaaaccgtcg cctacgatat agggaccatg atcataattg
    12361 taggtcaatt caatctcaaa gccatctcct tcaagtgcta agtaaaccaa ggtaaacttg
    12421 tgctctggat agtccttacg acgggtttcc ttaaatccaa atgcttcttc ataaaatttt
    12481 tgagatgctt ctaagttctc aacacgcaaa caagcgtgca acattttttt acttgccata
    12541 ataaattcct cctaccattt aataccacca gtataccata ctctctatga aaaatccgat
    12601 attcaaaact aaaaaagcct gagcgaactc aggccttaaa tgaattatgc ttccaagtag
    12661 tactctttca caatgttcaa gttttcatcc aattcgaata caagtggtgg gaagtttgga
    12721 atttccacat ccatgatttc gtcatcagac aattgtttga tgtgttttac aagggcacgg
    12781 attgagttac cgtgtgcacc cacgaatacg tttttaccgt ctttcaatgc tggagcaatt
    12841 ttatcttccc agaatggaag ggcacgctca agcgttactt tcaagttttc tgcatctgga
    12901 ataactgaat catcaaggtg tgcatagcga cggtcagtgt gtgctgaatg ctcatgatct
    12961 ttagccattt ctggtggcaa agtatcataa gaacgacgcc agatgtgaac ttgttcatca
    13021 ccaaattcag cagctgcttc agctttgttc aagccagtca aaccaccgta gtgacgttcg
    13081 ttcaaacgcc atgatttttc aactggtacc cacaattgat ctgcagcttc aagagccaag
    13141 tttgtagtct tgatagcacg tttcaataca gatgtgaaag caaggtcaaa ttcgatacct
    13201 gcttctttga tcaatttacc tgcatcgatt gcttgttgtg tacctttttc agacaaatca
    13261 acatcagccc aaccagtgaa aaggttagct ttgttccatt cagactcacc atggcgagca
    13321 aaaaccaatt ttaccattta tggattctcc tttaaatttt gaggttcccc tcttatactt
    13381 ctctatttta cacgaaaagg taaaaaaatg ccagcctttc cacaatctgt gtacgctttc
    13441 attactcttt catgtaaatt cagtctctag acctatcttt cttcttagaa aaaatacaaa
    13501 ataatggtat aatagtaagg ctgaacacga cagttcaaaa cgataccact ataggagtta
    13561 tcatgaaaaa agtagctatc gtgtctgcct atcgttctgc catcggcagt ttcggaggta
    13621 gtctgaaaga tattgaaatc gctgatttag gggcgcaagt cctcgaaaca gccttagcta
    13681 ataaaaacat ccctgcagac agcgttgatg aagttatctt cggaaatgtt ctttcagcag
    13741 gacaaggaca aaatattgca cgtcaaattg ctattcgtgc aggcattcct cagacagcct
    13801 ctgcctatgc agtcaataaa gtctgtggtt ccggtctcaa atcagtcctc ctagctgccc
    13861 agtcaatcat gctaggtgac aatgatgtag ttgtggctgg aggcattgaa attatgagcc
    13921 aggcacctta tctatccaaa actagccggt ttggtagtaa atttggtcat attagtctgg
    13981 aagattccat gttaacagat ggtttgacag atgccttcaa cgactatcac atgggcatta
    14041 ctgcagaaaa tgttgctgaa cgctatcatg tcagtcgcgc agagcaagat gcttttacat
    14101 acagtagcca agagaaggct gcaaaagcta ttgctgaggg aagatttgtt gacgaaattg
    14161 ttcctattag attaaaaaat cggaaaggcg aaactatttt tgccacagat gagtaccctc
    14221 gtctgacgcc aattgaaaaa ttggcaacac ttcggccatc ttttaaaaag gaaggaaccg
    14281 tcaccgcagc caatgcttct ggcatcaacg acggctgtgc tgtcttggta ctcatgtctg
    14341 atgaaaaagc cagtgagcta aatatccagc cactcgccta tattgaggcc tatgcaacaa
    14401 gcggtttaga tcctgcattg atggggcttg gacctattac tgctagccaa aaagcgctac
    14461 aaaaactgaa taaaacagtc gaggatatcg atctattcga actcaatgaa gcattcgcag
    14521 cccaatcaat tccagttgtc aaacaactcg gcattgatcc tgcaaaagtc aatgtcaatg
    14581 gtggtgctat agcccttgga catccaatcg gagctagtgg tagtcgaatc ctagtcactc
    14641 tgattcacga attaatcaag caagaaaaag agcttggtct ttgctcactt tgtatcggag
    14701 gcggtcaagg catctcccta atcgtttcca atgcgcaaac aaattaacct atcatttgta
    14761 cacaaaggat atattatgaa catcggtatt gataaaatcg gatttgcggc acccgattat
    14821 gttttagatt tggctgattt agcccaagcc cgcaatgtgg acccaaataa atttaagatt
    14881 ggtcttcttc agtctgaaat ggctgtagct cctgtcactc aagatattat cagtcttggg
    14941 gcaaaggctg ccgaagccat tctgacagaa gaagataaac aaaccattga catggttatt
    15001 gtcggcacag aatctagcgt agatcaaagt aaggctgccg ccgtcaccat tcatggatta
    15061 ctcggtattc agcccttcgc tcgctccatc gagatgaagg aggcttgtta cggtgcaacg
    15121 gctggactaa gtcttgccaa aagccatatc gctcaattcc cagaatcaaa agtcctagtc
    15181 attgctagtg atattgcaaa gtatggagtc gcttctggcg gtgaacctac ccaaggtgct
    15241 ggggcagttg ccatgttggt aaccgcaaac ccacaaattt tggtcctcaa caatgataat
    15301 gtctgccaga cacgtgacat ctacgacttc tggcgtccta actacgacaa atatccacgt
    15361 gttgatggta aattttcaac tgaacagtac accgattgct tgacgacaac tttcgactac
    15421 tatcaacaaa aaacaggcaa aaccctcaac gattttgctg ccatgtgctt acatattcct
    15481 ttctccaaac aaggtcttaa aggattgcaa gctattgctc aggatgaaga aactcttagt
    15541 cgcttgacag aacgtttcca ggaagctatc gtctataata aagtcgtcgg caacatctac
    15601 actggctcta tcttcctctc tcttctttcc cttttggaaa atagcagagc gttagagaca
    15661 ggggatcaaa tcctctttta tagctatggt agcggcgctg tctgtgaaat tttcagcggt
    15721 caactcgttg aagattatcg cgaccactta caagaaaatc gtttagaaca actcaatcag
    15781 cgtacaaaac tcagcgttaa ggaatatgaa caagtcttct ttgaagaaat tacccttgat
    15841 gaaaccggtt catcccttaa cctaccagaa gaccaaagtc cctttgccct tatcaaagtg
    15901 gacaatcaca aacgtatcta tcgtaaataa gcattttacc tatcattcat tggaataatg
    15961 aatgataggc tgatcgaatt tccagcacac ttttcacagt tagcgtcaac atctcagcgc
    16021 agtggttgat tggcagattt attcgtgtta ccacactcta aatctgacct aatcaactgt
    16081 gcgggggcgg gaagacgaac tcttttctat cagtcaagtt ctttcccgct ccccaaaacg
    16141 gcgccctttg gctctcacca aggggcgctt tcctatagaa agaggaacct atgtcaactt
    16201 tttccggatt ttataaaaaa tcacgccaag agcgcattga tattctccgt cagaatcgtt
    16261 ccctatccga agatagctta gacattctat acaaagacga aaacctgcca gaagcaattg
    16321 cgggaaagat ggcagaaaat catctaggaa cgttttcctt acctttttct gtacttccag
    16381 aacttctagt ggatggacaa acttactctg taccgatggt tactgaagaa ccttctgtgg
    16441 ttgcagctgc atctttcgga gccaaaatca tcgctaaatc tggcggtttt acaacgacta
    16501 ttcacaaccg tatcatgatt ggacaagttg ctctctacga tatttcggac catagcagag
    16561 cgacacaggc gattcttgac cataaggaga gtattttaga aactgccaat caagcacacc
    16621 cttccattgt caaacgaggg ggcggtgcaa gagaattgac tgttgagagt aaggatgagt
    16681 ttttgattgt ctatcttcag gttgatgtac aagaagccat gggagccaat atcctcaata
    16741 acatgttaga agctattaag gatgacttag aagagctcag caagggacag gctttgatgg
    16801 gaatcctctc caactatgca acggagtcac tggttaccgc ccaatgccat attgccatct
    16861 ctagtctggc taccagctct gccattgctc aagaaactgc tcaaaaaata gccctggcaa
    16921 gcaaactagc acaagtggac ccttatcgtg ctgcgacaca taacaagggg atttttaatg
    16981 gtatagatgc tgtcgttatc gctacaggaa atgactggcg ggcagtcgaa gccggggccc
    17041 atgcttatgc tagccgtgat gggcaataca aggggctttc tacttggtcc attgatggcg
    17101 agcacttggt cggctccatc accctacctc ttccaattgc ctccgtcggt ggctctatcg
    17161 ggctcaatcc aaaggtagca gttgcttttg accttctcca acaaccaaag gcacgccaat
    17221 tagcttctat catcgcttct gtcggtctct gtcagaactt tgctgccctg cgtgctcttg
    17281 ttacttcagg gattcaggct ggtcacatga agctccacgc aaaatcccta gcgctacttg
    17341 ctggcgccga ggagcatgaa gtagatcaac tagcccaact cctgcggaaa gaaaagcaca
    17401 gcaacctaga aactgctcaa aacttattag caaaaatgag agaacattaa aaaagaggga
    17461 ctccctcttt tttaatttat tatttgctcc atacggaact tttgtggttt cattccttgg
    17521 cgttcataaa aacggacagc tcctgcattg tcagcccata catccaaggt aagattgtgg
    17581 caaccctgct ccttagcata ggaaagagca aattcataca gttgctcacc aattttttga
    17641 ccacgcgccg aattggctac acacaaatca tcaataaaca aggttttaac tggttctaaa
    17701 acatcgcctg ttgtggtaga taattcacaa aaaagatgcc caaccacttg tccctctaac
    17761 tcaaagacaa aaataggctt atctggattt tccaaaagtg ctactaattc agcctcagaa
    17821 aatttttgac ctgcactttt aaaaatatct ggtcgtacct ggtgatgcac ctgtaaaata
    17881 tcttgaagca attcatttaa taccgaaata tcagactgtc tagctttacg gattgtcata
    17941 atactctcct tttgagtttt tattattata gcacatgttc attttttaac aaaataggtc
    18001 tacagaccga tgaagaattt gaaaattcga gtatactata ggtgtatcct aaaatttttc
    18061 ataaatctcc taaaaaatca gctggccaag gctggttttt tgttgtctta aaacgagaat
    18121 gtgtagtgta gaaaactact aatactcttc gaaaatcaaa attatccgtt gtcaacttgc
    18181 cttgatgaac tccagttcta tcttcggctt cgtttcctag gctacttttg attttcattg
    18241 agtataaagt tttgcccctt atttgcccct taaacaagaa aaaa
//
LOCUS       DAZQCJ010000032        18157 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000032 DAZQCJ010000000
VERSION     DAZQCJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18157
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.032"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(70..1221)
                     /gene="recA"
                     /locus_tag="ACHU66_001014"
     CDS             complement(70..1221)
                     /gene="recA"
                     /locus_tag="ACHU66_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR3389306.1"
                     /translation="MAKKPGKKLEDITKKFGDERKKALDDALKSIEKDFGKGAVMRLG
                     ERAEQKVQVMSSGSLSIDIALGAGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKDGG
                     IAAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSV
                     AALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTVAIFINQLREKVGVMFG
                     NPETTPGGRALKFYASVRMDVRGNTQIKGTGDKKDQNVGKETKVKIVKNKVAPPFKEA
                     VVEIMYGEGISRTGELIEIGSNLGIIQKAGAWYSYNGEKIGQGSENAKKFLADNPAIF
                     DEIDRKIRVHYGLIEADGVEEVATEEAPVVAEEIQDVILDLDGGIELED"
     gene            complement(1273..2454)
                     /locus_tag="ACHU66_001015"
     CDS             complement(1273..2454)
                     /locus_tag="ACHU66_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFR3389307.1"
                     /translation="MKAELIAVGTEILTGQIVNTNAQFLSEKCAELGIDVYFHTAVGD
                     NEQRLLSVLEVARQRSDLVILCGGLGPTEDDLTKQILATFLGRNLVFDELAMAKLDRF
                     FASRPGRVRTPNNERQAQIVEGSQALQNPAGLAVGGMIEQDGVTYIVLPGPPSELKAM
                     FSESLLPLLSQSQQQLYSRILRFFGIGESQLVTVLADLIDKQTDPTLAPYAKVGEVTL
                     RLSTKATSQEEANLRLNQLEEEILQHDKLADYFYAYGEDNSLVKTVATRLAEKRQTIA
                     IVEQGTGGLLQAELSLALADQPYFSGGKVIGQLGTESGRLSEEADCIRQELQADLGLA
                     VSVLIKPESTEDNVLAKVYLTLATTSGISQKEIDLGGYSWQYLRQLACLQAWDFVRNT
                     L"
     gene            complement(2491..3060)
                     /locus_tag="ACHU66_001016"
     CDS             complement(2491..3060)
                     /locus_tag="ACHU66_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR3389308.1"
                     /translation="MSRCAWVNLNNPLYIAYHDEEWGKSLHDEQSLFELLCLESYQAG
                     LSWEIVLNKRQAFRSAFFNYDIQKVAAMTDSELDSLLTNPDIIRHKAKLYATRANAQA
                     FLRVQEEFGTFDTYLWSWVDFTPLDNPIKSFRELPTKSELSERLSKDLKKRGFKFVGP
                     VCIYSYLQAAGLLNEHEETCEIGKKLRTN"
     gene            complement(3314..3904)
                     /gene="ruvA"
                     /locus_tag="ACHU66_001017"
     CDS             complement(3314..3904)
                     /gene="ruvA"
                     /locus_tag="ACHU66_001017"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR3389309.1"
                     /translation="MYDYIKGILTKITAKYIVVETQGVGYILQVANPYAYSGQVQQEV
                     TVYTHQVIREDAHLLYGFATENEKSVFLSLISVSGIGPTTALAIIAVDDNDGLVRAIE
                     QKNITYLTKFPKIGKKTAQQMILDLEGKFVMSEEAGPVQQVAPSSENIALDEAMEAME
                     ALGYRPAELKKIKKFFEGTNDTAENYIKSALKMLMK"
     gene            complement(3943..5880)
                     /gene="mutL"
                     /locus_tag="ACHU66_001018"
     CDS             complement(3943..5880)
                     /gene="mutL"
                     /locus_tag="ACHU66_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR3389310.1"
                     /translation="MSHIIELPEILANQIAAGEVIERPASVVKELVENSIDAGASQIE
                     ISVEEAGLKMIQITDNGEGIAPDEVALALRRHATSKIKNQSDLFRIRTLGFRGEALPS
                     IASVSHMIIETATADSAHGLHLEAKGGVIEKEEPVSRPVGTQITVSDLFYNTPARLKY
                     VRSQQAELSHIVDVVNRLSLAHPEMAFALVNEGRELIRTAGTGKLRQAISGIYGIASA
                     KKMVEIEAEDLDFQISGYVSLPELTRANRNYISIFINGRYIKNFLLNRAILEGYGSKL
                     MVGRFPLAVISIEIDPYLADVNVHPTKQEVRISKEKELMTLIREAISQALKEQDLIPD
                     ALENLAQSSTRPKVKAEQGTLPLREPKIYYDTIKQDFFLKPDVVAEDVKPLEEDRQEI
                     VESPAENKPTSVQFAERQSVESEDQEHPNLSAKELAKLADKLDREATSTFPELEYFGQ
                     MHGTYLFAQGKTGLYIIDQHAAQERVKYEYYREKIGQVDNSAQQLLVPYIFEFPQNDA
                     LDLVHKMDALRQVGVNLEEYGSNQFILREHPIWMKEEEIESGIYEMCDMLLLTDQVSI
                     KQYRAELAIMMSCKRSIKANHALDDYSARDLLRQLSYCQNPYNCPHGRPVLVHFSKSD
                     MEKMFRRIQENHTSLRELGKY"
     gene            complement(5998..7521)
                     /locus_tag="ACHU66_001019"
     CDS             complement(5998..7521)
                     /locus_tag="ACHU66_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol oxidase"
                     /protein_id="HFR3389311.1"
                     /translation="MTELDVVSDVVTHPEETYRRSRRASRRQQVQLNGEFHERVLKTK
                     YWPALVWSLVLSVFSVANPLLMPFATNMQTQNLYAGMAMANGQIPYGDFFGTSGLLFY
                     LLAFLGHLGGTFIIFGILQFIALLIAGVYFYKVVAYFSQSEHLATSSSHWFYVFIFAL
                     GFGGMYAEMFALPFLLTSVWFLVRYFENAVRDEAFILYGIDAALVFLIYPKSLILWLV
                     AGLVLFIFNIQHRQVTRGIYQLLATIFGFLLILYAVGYYAFEAQILGTAIQQTFLYNL
                     QLDFHHSYLYLALAIVSVFLLLSGFFKSFIQMVFSFKQGRHTYIKVLLLLTFLVQCVF
                     IIGNANFQWSQLILLLPYGFAMSVVYLRDEDVEDYRGYLRRQFFLPLAICLGIIAQPA
                     YLYLVQGDLRTDREQVANYIGEQTKDSDKIYVWDNSASIYLSSQRLSAATITTAEPYL
                     NTDDNKNSLMYNINKNEAKFVVVNKNLPILDEIKTNLESQYQSVQTTDYFTIYQKNE"
     gene            complement(7690..8931)
                     /locus_tag="ACHU66_001020"
     CDS             complement(7690..8931)
                     /locus_tag="ACHU66_001020"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFR3389312.1"
                     /translation="MLENWLNTKQGQVFHYKMEKIEYALELLGNPQFAVPVIHVAGTN
                     GKGSTIAFMRQLFQTHGLRVGSFVSPHMVSVHDRICIDSQPISDHDFQHYLQKVYDLE
                     QEVATRYEPFRYFEVMVLIMFLYFEAQQPDVALVEVGIGGLLDTTNVVAPALSVITSI
                     GMDHQDLLGSTLGEIAEQKAGIIKENVPVVLGPLCPETTAICRHIAQDKQASVYQFGQ
                     EFTYKAGQFSNADLELSELVLGLAGHHQEENAAVALQTFLLYMTNIQKDIQPQLIQQA
                     LAQTSWPGRLELVAQEPKIYLDGAHNVPAIERLIEFIQVQEEPVTILFSALRRKDFQE
                     MLELLDEKLPHTALVLTSFAYDGALSEENRQGRDYVENYRQFIEAWQSSKQGILIVTG
                     SLYFISEVRRIFKNEYLFANK"
     gene            complement(9196..10113)
                     /locus_tag="ACHU66_001021"
     CDS             complement(9196..10113)
                     /locus_tag="ACHU66_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR3389313.1"
                     /translation="MNDKEFGQRVRQLRESASMTREQFCDDELELSVRQLTRIEAGAS
                     KPTFSKIQYIATRLGMGLYELMPDYVSLPERYSKLKFDVLRTPTYGNEDLAEKRDAMM
                     TEIYDDYYDELPEEEKIVVDTLCSLFDVLDTDSQEYGKEILDDYLHQSYHRSKLSIND
                     LMILRLFIEHCQLEDLSVGTSNYALFTDLIEKLPQSIYDVHSESLFIVRDLFLAIVRI
                     LFSKELYNHIPVYIEKIENIMELSQDFQKKPILNLVKWKYELKVQHNHEIAERYYNEA
                     ITFASLLNQFHLKEKLQMEWEKDTQSLKR"
     gene            complement(10360..10998)
                     /locus_tag="ACHU66_001022"
     CDS             complement(10360..10998)
                     /locus_tag="ACHU66_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFR3389314.1"
                     /translation="MTTPTFIWDLDGTLLDSYEAILAGIQETYEQFDIPFDREEVRNF
                     ILRYSVKDLLVRDADKYGLDSDELNHVRATSLKEKNTQIPLMTGAVEILNWTAEQGIQ
                     NFVYTHKSDNAFQVLEDLGIRHHFTEILTSDSGFARKPSPEALLFLIEKYGLDKESTY
                     YIGDRLLDVETAINAGIRSINLQINGVEQNWKIVSLLDIKQILTDKSSHNNL"
     gene            complement(11000..11746)
                     /locus_tag="ACHU66_001023"
     CDS             complement(11000..11746)
                     /locus_tag="ACHU66_001023"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR3389315.1"
                     /translation="MAEQMRRVARLFGDWPETIIWTCLEGTMGDIYVDDSQSPQSALA
                     LYGRQSFFGFLAGQPHRDLLKICEGKNIILVPQNQAWSDLIEEVYGDGVRFFTRYATK
                     KDTEFDLGHLQKLVDDLPESFDMKLIDRNLYETCLVEEWSRDLVGNYIDVEQFLDLGL
                     GCVILHKGQVVSGASSYASYSAGIEIEVDTREDYRGLGLAKACAAQLILACLDRGLYP
                     SWDAHTLTSLKLAEKLGYELDKAYQAYEWR"
     gene            complement(11746..12747)
                     /gene="ruvB"
                     /locus_tag="ACHU66_001024"
     CDS             complement(11746..12747)
                     /gene="ruvB"
                     /locus_tag="ACHU66_001024"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR3389316.1"
                     /translation="MTNRILDMEQMQDEEYVERTLRPQKLNEYIGQDKVKDQLKIFIE
                     AAKLRDEALDHTLLFGPPGLGKTTMAFVIANELGVNIKQTSGPVIEKAGDLVALLNDL
                     EPGDVLFIDEIHRMPMAVEEILYSAMEDFYIDIMIGAGEASRSVHLELPPFTLIGATT
                     RAGMLSNPLRARFGITGHMEYYELADLTEIVERTADIFDMEITHEAAIELARRSRGTP
                     RIANRLLKRVRDFAQIMGDGLIDDNITDKALTMLDVDREGLDYVDQKILRTMIEMYGG
                     GPVGLNTLSVNIAEERETVEDMYEPYLIQQGFLMRTRTGRVATAKAYEHLGYPYTEK"
     gene            complement(13042..13215)
                     /locus_tag="ACHU66_001025"
     CDS             complement(13042..13215)
                     /locus_tag="ACHU66_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389317.1"
                     /translation="MKQTTYTLSLGGNDSGDSKVSKMEKKFEKYMEGIAERLRRSGKR
                     SIDKWFIRVRIEE"
     gene            complement(13317..15029)
                     /locus_tag="ACHU66_001026"
     CDS             complement(13317..15029)
                     /locus_tag="ACHU66_001026"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3389318.1"
                     /translation="MNRRLVGVLLKSLNKTMFLFGIALSALGVGMGLFLPQFIGQLLD
                     QTYLSNLLTRPELLAGFILFFVSVYTVQALSNYFIGRSGGNALKQLQQYIYESLLTTY
                     VKDLDQYQSGDLASRLTNDMSVVLNFITVILPNFLMNGLMVIGSIYFLWTISPWLTGL
                     SLLLLPMLSMVMIPMNQRLEGYYSAYQEGLGQVSSRISHKFTTIRLMKAFQGEKHEQR
                     EMGKSFQKLSQTFERMIRLSAVQHTLVSSLMTGFIILMLLVAGIEVTKGVMTMATLTT
                     FVLYMMQLIDPVTDIASSLNELTEFQAVSKRLVELLELTKEEQADTELVDEASIQLEN
                     VRFSYHTDPVLNGVSVRIPEGAHVAIVGPSGAGKSTIFALLMKYYQDYQGEIRIGQQC
                     LSDISTREMRRLISFIPQDNTLFHGTIRENLLYGKNESVSEERIAHILKELGLSHLVA
                     ELEDGLDTRISENGTGLSEGQKQRFSIARALLLEHPIYLLDEATASLDTVTERVISKA
                     IDRLTAGKTRLTIAHRLHTVREADAILVLDKNGQVADYGPHQQLVERNHLYQDFLRGL
                     PQAS"
     gene            complement(15032..15802)
                     /locus_tag="ACHU66_001027"
     CDS             complement(15032..15802)
                     /locus_tag="ACHU66_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389319.1"
                     /translation="MKQIFPAIFKTIWKRKETQIYLLFTLFPFIYLVTSFIEGSNFMQ
                     IHASEGSVSLVAFTNMMMSSVDSFILPSLTLYFLAISVFRKEVDEHTMFLYRDLGRKQ
                     IFWSKYLGLLATIVIFYGLFFATSAFVHYVRVIHLPFGSPSVFDTSLAESLSNVFCIV
                     AYLLKDILSVSLATALCLYLKNITTMIIGFLVTITMMILAVVGGPVAMIFPTSYMPLA
                     SEGLTGVGLAYLGAIGVTLVYALVFTKIAAKKFKNLEF"
     gene            complement(15808..16680)
                     /locus_tag="ACHU66_001028"
     CDS             complement(15808..16680)
                     /locus_tag="ACHU66_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3389320.1"
                     /translation="MLKINHLSKQFAGNEFYSLKDVSLEINKGEIVGLIGKNGAGKST
                     LMKLIAKSLKPSSGTITYKGTDIFSQDNLLADFGIMIDPVFYPEMTVMDNLKFYLDLH
                     GKKDLYPNIEKTLKLVELWEARNRKPKGFSFGMKQRTALAIALVAEPDFLILDEPFVG
                     LDPIGVQKLIDILKQWSSERQISMLISSHQLGELEALCSRYVYIESGELAEAFEGKSH
                     PSVLVQLNTTKNLDSVEALLNDHVVLEGEVLEISTATPTSELNQIFGVLARQELIVKL
                     EVKENHLKEIFTKG"
     gene            complement(16673..17101)
                     /locus_tag="ACHU66_001029"
     CDS             complement(16673..17101)
                     /locus_tag="ACHU66_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msa family membrane protein"
                     /protein_id="HFR3389321.1"
                     /translation="MIFILLLSLLYTCLTVVSSQLLLSLSVPILLLLTYLLPPIVNSI
                     CLGLLKDNSWKAWSAGIFPTMSMIFYAAFAYITSSNGQWAQFSQLNTVSDDVMSVEVA
                     SNLLAPSQILFVAVVYYGVAIATYFIQQTSRQKEKGVTYA"
     gene            complement(17116..17694)
                     /locus_tag="ACHU66_001030"
     CDS             complement(17116..17694)
                     /locus_tag="ACHU66_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFR3389322.1"
                     /translation="MVIRYIRPVALSIALIILASISYLLATSLVLFSPSQFLQVAYLF
                     SIMVGMPVGFILFPSMLTKRYQLCQELSEVKFSWKSFVLLAIAIFLINFWCIQSDEYV
                     NQFIIATCEEFLFRYLIYRILKSEYPTWLAMLVTSLLFGVLLHMNYPLLDNLIIRTPL
                     GLLFSVLATRFGLQYAIGGHWIYNLLVSRFPF"
     gene            complement(17704..>18157)
                     /locus_tag="ACHU66_001031"
     CDS             complement(17704..>18157)
                     /locus_tag="ACHU66_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HFR3389323.1"
                     /translation="NRTRLFNAKIISLVTMYASYVLLTMLTSAIAYFGFLNASGKVVS
                     DDWNNVQSTFLSIFATISINVIGILLVAMVSIKAKSLQAVLAGVFWSLFTTTAPFLIG
                     VRYVVPNGYAQMSFNQPLLAWSLVAAITTLYIVATYLKGRSNFEKLEF"
BASE COUNT         5438 a         4203 c         3344 g         5172 t
ORIGIN      
        1 aaacaacttc gtacacacct ataacagata tgcacaaata gttcaatatt aaatctattt
       61 tgttaggaat taatcttcta attcaatacc gccatcaaga tctaggataa catcttggat
      121 ttcttccgca acaacaggag cttcttcggt cgcaacttct tcaactccat ctgcttcaat
      181 caagccataa tgaacacgta tcttgcggtc aatttcatca aagattgctg gattatctgc
      241 taagaatttc ttagcatttt cagaaccttg gccaattttt tctccattat atgaatacca
      301 agcacctgct ttttggatga tgccgaggtt actaccaatc tcaatcaatt cacctgtgcg
      361 agaaattcct tcaccgtaca tgatttcaac aacagcttct ttaaatggtg gcgccacctt
      421 gttcttcaca atcttcacct tggtttcctt accaacgttt tgatctttct tgtcgcctgt
      481 ccctttgatt tgtgtgtttc cacgaacatc catacggaca gatgcataga atttaagcgc
      541 acgtccacca ggtgttgttt cagggttacc aaacatgaca ccgacttttt cacgcaactg
      601 gttaatgaaa atggctaccg tcttagtttt gttgatggat gctccgagtt tgcgcatagc
      661 ctgactcatc atacgagcct gcagaccaac atgactatca ccaatatcgc catcgatttc
      721 tgcacgtggt acaagggctg caaccgagtc aacaacaacc aagtcaaccg caccagagtc
      781 gatcagcttg cctgcaattt caagaccttg ttcccctgag tctggctgtg acaatagtaa
      841 ctcatcaata ttgacaccaa gagctgctgc ataggctgga tccaaggcgt gttccgcatc
      901 gataaaggca gcaataccac catctttctg ggcttgagct actgcatgaa gggcaacagt
      961 tgtcttacct gaactctctg gaccataaat ctcaatgata cgacctttcg gatagccacc
     1021 cgctccaagc gcaatgtcga ttgacaaact acctgagctc atgacttgaa ctttttgttc
     1081 agcacgctca ccaagacgca taacagcacc cttaccaaaa tctttttcaa ttgattttag
     1141 ggcatcatcc agcgcttttt tacgctcatc gccaaatttc tttgtaatat cttctaattt
     1201 ttttcctggt ttcttagcca atgattttct cctcttttct tagtcaaact attatagcaa
     1261 atatctgcta tttcacaaag tgtttcgtac aaaatcccag gcctgcagac aagcaagctg
     1321 gcgaaggtat tgccacgaat aaccacctaa atctatctct ttttgggaaa tacccgaggt
     1381 cgtagccaaa gtgagatata cttttgcaag tacgttgtcc tctgttgatt ccggtttgat
     1441 aagcacagac acagccaaac ccaaatcagc ttgcagctcc tgccgaatgc agtcagcttc
     1501 ctctgatagc ctgcccgatt ctgtccctag ctgaccgatg acttttcctc cgctaaaata
     1561 cggctgatca gccaaagcca ggcttaattc agcttgcaag agaccacctg tcccctgttc
     1621 gacgatagcg atggtttgtc ttttctccgc caaacgagtc gctaccgttt tgaccaaact
     1681 attgtcctct ccgtaggcat agaaatagtc tgccagtttg tcatgttgta agatttcttc
     1741 ttccaactga ttcaaacgta gatttgcctc ttcttggctg gtcgctttgg tggacaagcg
     1801 taaagtcacc tctccaactt ttgcataagg agcaagagtt gggtctgtct gcttgtcgat
     1861 caagtccgcc aaaacagtca ccaactggct ttcaccaatg ccaaaaaaac gtaggatacg
     1921 cgaataaagt tgctgctgag attgggacag taaaggtaag agactctcag aaaacatggc
     1981 cttgagctca cttggtgggc caggcaaaac aatataggtt acaccatctt gctcaatcat
     2041 accaccaaca gctaaaccag ctggattctg tagcgcctga cttccctcca caatctgtgc
     2101 ctgacgttca ttatttggtg tacggacacg acctgggcga ctggcaaaga aacggtctaa
     2161 ttttgccata gccaactcat caaacacaag atttctacct aagaaggtcg ccaggatctg
     2221 cttggtcaag tcatcctcag ttggacccaa tcctccacag agaatgacca ggtcgctccg
     2281 ctgacgggct acttccagta cagacagaag cctctgttca ttatctccaa cagctgtgtg
     2341 gaaatagaca tcaattccca gctctgcaca tttctccgaa aggaactgag cattggtatt
     2401 aacgatttga cctgtcaaaa tctccgtccc aacggcgatt agttctgctt tcatattcct
     2461 cctctgtcta gggtgcgatt gctcgctcat ctaattagtt cgtaatttct ttccaatctc
     2521 gcaagtttct tcatgttcat tcaacagacc tgctgcctgc aaataagaat aaatacagac
     2581 aggacctaca aacttgaaac ctcgtttctt caaatctttg gacaatcttt ctgatagttc
     2641 acttttggtc ggtagctctc gaaaggactt aatagggttg tcaagaggtg taaagtcaac
     2701 ccaagaccaa agatacgtat caaacgtgcc gaattcctcc tgtactcgaa gaaaggcctg
     2761 agcattggcg cgggtagcgt atagctttgc cttatgacga atgatgtctg gattggtcag
     2821 aaggctatct aactcgctat ctgtcatagc cgcaaccttt tgaatatcat agttgaaaaa
     2881 agcagagcga aaagcctggc gtttattgag aacaatttcc caggaaagtc ctgcttggta
     2941 ggattccaag caaagcaact caaacaaaga ctgctcatcg tgaagtgact tgccccattc
     3001 ttcatcatgg taagcaatat aaagtggatt atttagatta acccaagcac aacgggacat
     3061 agaaactcct tttcgtgaat aactaaagtg cgaatcagga taaactgact gaaacacggc
     3121 tacgatactt tgtaaaattg tcaaactgaa tcctaaatat caccgaataa cgcagttgac
     3181 atctggcaaa aggcttcgtc aaaactcgcc ttttacctag ttgtcattgc ggaggtctca
     3241 caacgaaatt tctagcaaaa tttctgttcg gccgtctatt ttgctttgta tcgcttaacg
     3301 ccttagtatc ctattacttc atcagcattt taagggctga cttgatgtag ttttcagcgg
     3361 tgtcattggt gccttcaaag aatttcttga ttttcttgag ttcggctggg cggtaaccaa
     3421 gggcttccat ggcttccata gcttcatcaa gagcgatatt ttcactggat ggtgctactt
     3481 gttgaacagg acccgcttct tcgctcatga caaacttgcc ttccaggtcc aaaatcatct
     3541 gctgggctgt tttcttgcca atcttcggaa acttggtcag gtaggtaatg tttttctgct
     3601 caatagcacg aacaagtcca tcattatcat caacagcaat aatagccaga gccgttgttg
     3661 gaccaatacc tgatactgaa atcagactca gaaagacgga tttttcattt tctgtagcaa
     3721 atccgtagag caaatgagca tcttctcgaa tcacttgatg agtatagacc gtcacttctt
     3781 gctggacttg tccagagtat gcgtagggat tagcaacctg caagatatag cctactcctt
     3841 gtgtttctac cacaatatat tttgcagtta tttttgttaa aattccttta atatagtcgt
     3901 acatatactt cctttactaa tgcgtcaact ttttcaacta tgttaatact tgcccaattc
     3961 tcgcaaactc gtgtgatttt cctgaatgcg acggaacatt ttttccatat ccgatttgct
     4021 aaaatgtact aagactgggc gaccgtgcgg gcagttatag ggattttggc agtaagacaa
     4081 ttgtctcaat aaatcacgcg ccgaataatc atccaaagca tggttggcct tgattgaccg
     4141 tttacaagac atcatgatag ccagttctgc ccgatactgc ttgattgaca cctgatccgt
     4201 caagagcaac atgtcgcaca tctcataaat gccagactca atctcctctt ccttcatcca
     4261 aataggatgt tcacgcagaa taaattgatt agatccatat tcttccaagt tgacaccaac
     4321 ttgacgaagg gcatccattt tgtggactag gtcaagggca tcattctgcg gaaattcaaa
     4381 aatatagggc accaataact gttgagccga attgtcaacc tgtccaattt tctcacggta
     4441 gtattcgtat ttgaccctct cttgtgcggc atgctggtca atgatataaa gtcctgtctt
     4501 accttgcgca aataaatacg tcccatgcat ttgaccaaaa tattctaact ctggaaatgt
     4561 cgaggtcgct tcccggtcta atttatctgc cagttttgcc aattcttttg cacttagatt
     4621 aggatgctcc tgatcttctg attccaccga ctgacgttct gcaaactgaa cagatgtagg
     4681 tttattttcg gcaggcgact caacaatctc ttgcctatcc tcttcgagag gtttgacatc
     4741 ctcagcgacc acgtctggtt ttaagaagaa gtcttgctta atcgtatcat aatagatttt
     4801 tggctctctg agtggtaagg tgccttgctc tgcttttact tttggtcgag tacttgactg
     4861 agctaaattc tcaagagcat ctggtatcaa gtcctgctct ttaagcgctt gactaatcgc
     4921 ctctcgaatc aaggtcatga gttctttttc cttggagatg cgaacttcct gcttggtcgg
     4981 atgcacattg acatcagcaa gataggggtc aatttctata gaaatcactg ccaacggaaa
     5041 gcgtccaacc atcaacttac tgccgtaacc ttccaaaatc gctcgattca gcaaaaaatt
     5101 cttgatatag cgaccattga tgaaaatgga aatgtagttg cggttggcac gagtcaactc
     5161 tggcaaggaa acataacctg aaatctgaaa atccagatct tctgcctcaa tttcaaccat
     5221 ttttttggca gaagcaatcc cataaatccc tgaaattgcc tgacgaagtt tccctgtccc
     5281 tgcagttcta attaattctc gtccttcgtt tactaaggca aaagccattt ccggatgagc
     5341 tagactcaat cgatttacaa catcaacaat atgtgataat tcagcctgtt gactgcgaac
     5401 atatttgaga cgagcagggg tattgtaaaa taaatccgaa acagtaatct gcgtaccaac
     5461 tggacgacta actggctcct ctttctcgat gacaccacct ttggcctcca aatgaagtcc
     5521 gtgtgcagag tctgccgtag ctgtctcaat gatcatatga ctgacagaag caatggaagg
     5581 cagagcttct ccacgaaaac cgagggtgcg aatacgaaac aaatccgatt gattttttat
     5641 cttactggtg gcgtgacggc ggagggcaag cgctacttca tcaggggcaa tcccctcacc
     5701 gttatccgta atttgaatca ttttaaggcc agcctcttca acactaattt caatctgact
     5761 agcccctgcg tcaatggaat tttccaccaa ctccttaacc acactggctg gtcgctcaat
     5821 gacctcacca gccgcaatct ggttggctag tatttctggt agttcaataa tatgtgacat
     5881 catttctcct acatttcttt aataattttt cccgaatgct tatttttaca ttataccaca
     5941 tatccacttt ccctcctttt cccagatagg ctaaagaaaa aatgactgcc tgagcaatca
     6001 ttcattcttt tgataaatcg taaagtaatc cgttgtctga acagactggt actgtgattc
     6061 taaatttgtc ttgatttcat ctagaattgg caaattctta ttgacgacta caaattttgc
     6121 ctcattttta ttgatgttat acatcaagct atttttattg tcgtcagtat tcagataggg
     6181 ttctgctgta gtaatcgttg cggcagataa acgttgactt gaaagataga tgcttgcgct
     6241 attatcccaa acataaatct tatccgaatc tttcgtttgt tcaccaatgt aattggcaac
     6301 ttgttcacgg tcagttctca aatctccctg taccaaataa agataagcag gttgagcgat
     6361 aatacccaag caaatagcta acggaagaaa gaattgacga cgcaaatacc cacgataatc
     6421 ttctacatcc tcatcacgca ggtaaacgac tgacatcgca aaaccatacg gtaaaagaag
     6481 aatgagttgg ctccattgaa aattagcatt cccaataata aagacacatt gaactaagaa
     6541 agtcaataat aggagaactt tgatataggt atgtcttcct tgtttgaaag aaaacaccat
     6601 ttggatgaaa cttttaaaga atccagataa gagtaaaaat acagacacaa tcgccaaggc
     6661 taaatataga taggaatggt gaaagtctaa ctgtaagtta taaaggaaag tttgttgaat
     6721 agctgtcccc agaatctgtg cctcaaaagc ataataacct accgcatata gaatcagaag
     6781 aaaaccaaaa atggtcgcta ataattgata gattcctcga gtaacttggc ggtgttggat
     6841 attaaaaata aataaaacta gacctgcaac taaccaaagg attagacttt ttgggtaaat
     6901 taaaaatacc aatgccgcat cgattccgta gaggataaat gcctcatcac gcactgcatt
     6961 ttcaaaatag cggaccagaa accacacact agtcaaaaga aatggaagag caaacatttc
     7021 tgcatacatc cctccaaaac caagtgcaaa aataaataca tagaaccaat gactagaact
     7081 tgtcgctagg tgttctgact gactaaaata agctacaacc ttatagaaat agactcccgc
     7141 tatcagtaat gctataaact ggaggattcc aaagataatg aaagttcctc ccagatgacc
     7201 taagaaagca agtaaataga ataataaccc gcttgtacca aaaaaatcac cataagggat
     7261 ttgtccattt gccattgcca tccctgcata taaattttga gtttgcatat tggttgcaaa
     7321 tggcattagc agtggattgg ctacgctaaa aacacttaaa actaagctcc aaacaagagc
     7381 tggccaatac tttgtcttta atactctttc atgaaattca ccattcagtt gcacttgctg
     7441 tctgcgtgat gctctacgac tacgccgata agtttcctca ggatgagtaa ctacgtccga
     7501 gactacatct aattctgtca ttttcttctc cttggatttg ctttccttat tatatcaaaa
     7561 agcactcccg atgtcatttc ctatcgggag ttctggaaat ttttgcaaac tgttctaatt
     7621 ggcgaaaggt atggcgtttg ttctcttgaa attcttttaa aaaggctact ttttctgcat
     7681 gtgtcatacc tatttattcg caaaaagata ttcattttta aaaatccgac gaacttctga
     7741 gataaagtag agagagcctg tgacaatcaa aatcccttgc ttgctagatt gccaagcttc
     7801 tataaactgc cggtaatttt caacataatc tcgtccttgt cggttttcct ccgacaaggc
     7861 accatcataa gcaaagctgg ttaatacaag ggcagtatgt ggtaattttt cgtccaataa
     7921 ttccagcatt tcttgaaagt ccttgcgccg aagggccgaa aagagaatgg tgacaggctc
     7981 ttcttgaacc tgaataaatt caatcaagcg ttcgatagcg gggacattgt gggctccatc
     8041 caaatagatt tttggctctt gagcaaccaa ttccaaacga ccaggccagc tagtttgggc
     8101 aagggcttgt tgaatcaact gaggttgaat gtctttttga atattggtca tatagagtag
     8161 aaaagtttgt agggctacgg ccgcattctc ttcttgatga tggcctgcca gacccagaac
     8221 taattctgac agttccaagt ccgcattgct gaactgtcct gctttatagg tgaattcctg
     8281 accaaattgg tagacagagg cttgtttgtc ttgggcaatg tggcgacaga tggctgtggt
     8341 ttctggacaa agtggtccta agacgacagg cacgttttcc ttgataatcc ctgctttctg
     8401 ctctgctatt tcccctaaag tcgagcctag taaatcctga tggtccatgc cgatggaggt
     8461 gatgacgctg agagccggtg ccacgacgtt ggttgtatcc aaaagccctc cgatacccac
     8521 ctctactagt gccacatcgg gttgttgagc ttcgaaatag aggaacataa tgagcaccat
     8581 gacctcaaaa taacggaagg gctcatagcg tgtagcgact tcctgctcca agtcgtagac
     8641 tttctgtaaa tagtgctgaa aatcatggtc tgaaatgggc tggctgtcaa tacaaatcct
     8701 gtcgtgtaca ctcaccatgt gaggagatac aaaactaccg acacgcaagc cgtgagtctg
     8761 aaagagctga cgcataaagg caatggtcga tcccttgcca ttggttccag caacatgaat
     8821 gaccggaact gcaaactggg gattccctag cagttctagg gcatactcaa tcttttccat
     8881 cttgtaatga aaaacctgac cttgtttggt gtttaaccaa ttttctaaca tagccacctc
     8941 ctacatactg tctagtataa caaaaaagcc taacggatgt tggctttttg aaaatatcgt
     9001 actccacgac ttaaatcccc ataaaataaa cgctctatgc tgagtattca attatttatt
     9061 ttacccacca gttctcatca ccaagttgac caaaaccacc aaaaaattta aaaaattgaa
     9121 acataacata taatctcctt ttgttttatg aatctagtat aaaataaact ctagattctc
     9181 aacaggacaa aaatgtcatc tttttagact ctgcgtatct ttttcccatt ccatttgcag
     9241 tttttctttc aagtgaaatt gatttagtag gctggcaaat gtaatcgctt cattatagta
     9301 cctctcagca atttcatgat tatgttgtac ctttaattca tatttccatt tcacaagatt
     9361 tagaattggc ttcttctgaa aatcttgtga taattccatg atgttttcta ttttttctat
     9421 atagactgga atatgattat acaactcctt cgaaaataaa atacgaacaa tagctagaaa
     9481 taaatcgcgg acaatgaata aggattccga atgtacatca tagattgatt gaggcaattt
     9541 ctctatcaaa tctgtaaata aagcataatt ggaagttcca acagataaat cctccaactg
     9601 acaatgttca ataaataatc gcaaaatcat taaatcgttt atagatagtt tggaacggtg
     9661 atagctttga tgtaaataat catcaagaat ttctttaccg tattcctggc tatctgtatc
     9721 caatacatca aatagggaac atagggtgtc tacaactatt ttctcctcct caggcaattc
     9781 atcataataa tcgtcataga tttctgtcat catggcatct cgcttttccg ccaaatcttc
     9841 attaccataa gttggggtgc gaagcacatc aaacttcagc ttggaatatc tttcgggtaa
     9901 agatacataa tctggcataa gctcgtaaag tcccatacct aaacgagttg caatatactg
     9961 aatctttgaa aaagtcggct tggaagcacc tgcttcaata cgagttaatt ggcgcacaga
    10021 gagttccagt tcatcgtcac aaaactgttc acgtgtcata ctagcagatt ctcgtaattg
    10081 acgcacacgc tgtccaaact ccttatcgtt cataaaccac acctccaaat atacttttta
    10141 aattatatca aatatattac acaaattcca tatcatgctc ttcttaaaac agacggctgg
    10201 attcgttttt attatagctt gaagccataa caagataaaa gcccgatgtt acgggcattc
    10261 gtcaagtaat catagcacta acctgaacga agttcagcgg acgtctttag acatcgctgg
    10321 agagaaacag cttatagctg gtgtacaagc cacccgtttt cacaggttgt tatgacttga
    10381 tttatctgtt aatatttgtt ttatgtccaa taaagaaaca atcttccaat tctgctccac
    10441 gccattaatt tgcagattga tactacgaat acccgcatta atagccgttt ccacatcgag
    10501 cagacggtcg ccaatatagt aggtgctttc cttatccagc ccgtattttt cgatgagaaa
    10561 tagcagagct tctggacttg gtttgcgggc aaagccagaa tcgctggtca agatttctgt
    10621 gaaatggtgg cgaatgccca agtcctccaa gacctgaaag gcattgtcac tcttgtgggt
    10681 atagacaaaa ttttgaatgc cttgttccgc agtccagttt aagatttcca ccgcacctgt
    10741 catcaaggga atctgcgtat tcttctcctt caaggaagtt gcacgcacat gattgagctc
    10801 atcgctatcc agaccgtact tgtccgcatc acgcaccagc aaatccttga cagaatagcg
    10861 gaggatgaaa tttcttactt cctctcgatc aaaagggatg tcaaattgtt cataggtttc
    10921 ttggatacca gccaaaatgg cctcgtaaga atctaaaagt gttccatcca aatcccaaat
    10981 aaaggtcggt gttgtcattc tatctccatt cgtatgcttg ataggcttta tctagttcat
    11041 agcccagttt ttccgcaagt ttcaaggaag tcagtgtatg ggcgtcccag ctagggtaga
    11101 gcccgcggtc taaacaagct aaaattaact gagccgcaca ggcttttgcc aaacccagac
    11161 ctcgatagtc ttccctagta tccacttcta tctcaatccc agctgaatag ctggcatagg
    11221 atgaagcccc tgagaccacc tgtcccttat gcaagatgac acagcccaga cccaagtcca
    11281 aaaattgttc cacatctata taatttccca ccaaatcccg tgaccattcc tctaccagac
    11341 aagtctcgta cagattacgg tcaatcagtt tcatatcaaa gctttcaggc aagtcatcaa
    11401 ccagtttctg taaatgccca aggtcaaact cagtgtcctt tttcgtagcg tatcgagtaa
    11461 aaaaacgaac tccgtctccg taaacttctt ctatcaagtc tgaccaggct tggttttggg
    11521 ggactagaat aatattcttt ccctcacaga tttttagcaa gtctcgatgt ggctggccag
    11581 ctaaaaaacc aaagaagctc tgccgtccat agagagctag agctgactgg ggtgactggc
    11641 tgtcatcaac ataaatgtct cccatagtac cttctagaca ggtccagatt atcgtttcag
    11701 gccagtctcc aaataaacga gctactcgtc tcatctgctc tgccattatt tttccgtata
    11761 gggatagccc aggtgttcgt aagccttggc tgtcgcaacc cgccccgtcc gtgtccgcat
    11821 gaggaagccc tgctgaatca ggtaaggttc gtacatgtcc tccaccgtct cacgctcctc
    11881 ggcaatattg accgacagag tgttgaggcc gacgggaccg ccgccgtaca tctcaatcat
    11941 ggtgcggaga atcttctggt ccacgtagtc cagcccctcg cggtccacat ccagcatggt
    12001 cagggccttg tccgtaatgt tatcatcaat caggccgtct cccataatct gtgcgaaatc
    12061 ccgcacccgc ttgagcaagc ggttggcgat acggggagtt ccacgagacc gacgagccag
    12121 ctcaatagca gcttcatgag taatctccat gtcaaagatg tccgctgttc gctcgacaat
    12181 ctccgtcaaa tcagccagtt cataatactc catatgaccg gtaataccaa aacgggcccg
    12241 cagaggattg gatagcatac ccgcacgagt ggttgctcca atcagggtaa acggtggcaa
    12301 ctccaaatgc acggagcgac tggcctcccc tgctccaatc ataatgtcga tgtagaaatc
    12361 ctccatagca gagtagagaa tttcctcgac cgccatgggc atacggtgga tttcatcgat
    12421 aaagaggacg tcgccaggct ccaagtcgtt gagaagggcc accaagtcac ccgctttttc
    12481 aataacagga ccactggtct gcttgatatt gacgcccaat tcgttggcaa tgacaaaggc
    12541 catggtggtc ttccccagac ccggtgggcc aaaaagaagg gtatggtcca aggcttcatc
    12601 acggagcttg gccgcctcga taaagatttt gagctggtcc ttgaccttat cctgaccgat
    12661 gtattcattt aatttctgcg gacgcagggt acgctcaaca tactcctcgt cctgcatttg
    12721 ttccatatct aaaattcgat ttgtcatgaa accattatag cataaaatga aaataccaga
    12781 ctcagtgagt ccagtattct caggagatta tataatccct agtccttgtc gcttgcgact
    12841 tagagatacg gattgaaaag atatgccagc cgtttgagct ttagctctta cggatgacga
    12901 tattgaaact atttcagtaa aagtctcgct atttctagct tgaataaagt ataactcgcc
    12961 aaacttaagg gaaactaaaa gagaattcat tctatttttc actatttata aaaacgtctg
    13021 tgaattttgg tcatagctac tttactcctc aatccgcaca cggataaacc atttatcaat
    13081 actcctcttc ccagaacgcc gtaaccgctc tgcgatcccc tccatatatt tttcaaactt
    13141 tttttccatc tttgaaactt tcgaatcccc gctatcattt cctcccaaag ataatgtgta
    13201 agttgtttgt ttcattctct tctcctatct atttattgtg caagtaatca tcagtattcc
    13261 attttattat taggtaaccc tagacttaga ctagtcggtc catcgactcc cattcactaa
    13321 ctggcttgcg gtaagcctct taagaaatct tgatagagat gatttctctc cactaactgc
    13381 tggtgaggac cataatcggc tacttgccca tttttatcta agaccagaat cgcatccgcc
    13441 tcacgcactg tgtgcaaacg gtgagcaatg gttagccttg ttttcccagc tgttaaacgg
    13501 tcaatcgctt tgctaatgac acgttctgtg acagtatcta aacttgctgt cgcctcatcc
    13561 aacaaataga taggatgctc taacaacaag gctctcgcaa tgctaaatcg ttgtttttgg
    13621 ccttccgaca aacctgtccc attttctgaa atccgagtat ctaaaccatc ttccaactca
    13681 gctactaaat gagacaatcc caactccttg agaatatgag caatacgctc ttcagacact
    13741 gactcgttct ttccatacaa tagattttct ctaatcgtcc catggaagag ggtattatct
    13801 tgaggtataa aggaaatcaa tcttctcatc tccctggttg aaatatcact taagcattgt
    13861 tgaccaatcc gaatctctcc ttggtagtct tgatagtatt tcatcagcaa ggcaaaaata
    13921 gttgattttc ctgctccact tggaccaaca atcgccacat gagctccttc aggaattcta
    13981 accgatactc cattcaaaac cggatctgta tgataggaaa accgaacatt ttccaactgg
    14041 atactagctt catcaaccaa ctctgtatct gcctgttctt ccttagtcag ctccagcaac
    14101 tctaccaaac gtttagacac cgcctgaaac tccgttagtt catttaagga agaggcaata
    14161 tctgttactg gatcaatcag ttgcatcatg tacaatacaa aggttgtcaa tgtagccatg
    14221 gtcatcactc ccttggtcac ttctatacct gctaccagca acatcagaat gataaaaccc
    14281 gtcatcaagc tactaaccaa agtatgttgc accgctgaca gtctaatcat tctctcaaag
    14341 gtttgcgaca gtttctgaaa agacttgccc atctctcgtt gttcatgctt ttctccctga
    14401 aaggccttca tcaaacgaat cgttgtaaat ttatgactaa ttctactaga aacctgccct
    14461 aatccctctt gataagcaga gtagtaacct tccaaccgct gattcattgg aatcataacc
    14521 attgacaaca taggaagcaa caagagacta agacctgtga gccacggact gatggtccac
    14581 agaaaataaa ttgacccgat aaccatcaaa ccattcatca agaaatttgg aagaataacg
    14641 gtgatgaagt tcagaacaac cgacatatca ttggtcaaac gactggcaag atcaccggat
    14701 tggtactgat ccaagtcctt tacatatgtg gtcaacaagg attcataaat atattgttgc
    14761 aactgtttga gtgcgttacc accactacgc ccgatgaaat aattggatag ggcttgtacc
    14821 gtataaaccg atacaaagaa aaggatgaag ccagccagca attctggtcg tgttaataga
    14881 ttgcttaaat aggtttgatc tagcaactga ccaataaact ggggaaggaa taagcccatt
    14941 ccaaccccaa gagcactcag cgctattcca aacaagaaca tcgttttatt gagcgatttc
    15001 aacaatactc ctacgagtct tctattcata cctagaactc caaattcttg aattttttcg
    15061 cagcaatttt agtaaatacg agtgcgtaaa caagcgtcac accaatagct cctaagtaag
    15121 caagtcccac tcctgttaga ccttcgctcg ctaaaggcat ataactagtt ggaaaaatca
    15181 tcgcaactgg accgccaaca actgctaaaa tcatcatcgt aatcgtcacc aagaatccta
    15241 tgatcattgt ggtaatattt ttcagataca aacaaagagc agttgccaaa ctaaccgata
    15301 aaatatcttt tagtaaataa gcgacaatac aaaacacatt actcaaactc tctgccaaac
    15361 tagtatcaaa aacggacgga ctgccaaaag gcaggtgaat tactcgaaca taatgtacaa
    15421 atgcagatgt cgcaaagaag agaccataga aaataacaat tgttgctaag agacccaaat
    15481 atttagacca gaaaatctgt ttccgcccaa gatctctgta taaaaacatg gtatgttcat
    15541 ctacttcctt cctaaagaca gagatagcca agaaatacag ggtcaaacta ggaagaataa
    15601 aactatccac agagctcatc atcatattcg taaaggctac aaggctaacc gacccctcac
    15661 tagcatgaat ttgcataaaa ttcgaccctt caataaatga agtgaccaga taaataaaag
    15721 gaaacaaagt gaacaggaga taaatctgcg tctcctttcg cttccaaatc gttttaaaga
    15781 tagctggaaa aatttgtttc atactcacta ccctttcgta aagatttctt tcaagtgatt
    15841 ctctttcact tctagtttca caatcaattc ttgacgagcc agcacaccaa aaatctgatt
    15901 tagctcactg gtaggtgtag cagtggaaat ttctaacact tccccctcta gcaccacatg
    15961 gtcattgagc aaagcctcta cagagtctag gtttttagtc gtattcaact gcacgagcac
    16021 acttggatga gatttgcctt caaaagcctc tgccaattct cccgattcga tatagacata
    16081 gcggctgcac aaggcctcca gctcccccag ttggtgactc gaaatcagca tggaaatttg
    16141 tcgctcactc gaccactgct tcaaaatatc aatcaatttc tgaacaccaa tcgggtccaa
    16201 gcccacaaac ggctcatcta agataaggaa atctggctcc gcaaccaagg caattgccaa
    16261 agcagtccgt tgtttcatac caaacgagaa tcccttgggt ttccgattgc gagcttccca
    16321 caattctact aatttcaaag tcttctcaat atttggatag aggtcttttt tcccatgcag
    16381 atccaaataa aacttaagat tgtccataac tgtcatttct ggatagaaga cgggatcaat
    16441 cataatccca aaatctgcca acaaattatc ctgactaaaa atatccgtcc ccttataagt
    16501 aatggttcct gaacttggtt ttagggactt ggctattaat ttcatgagcg tggattttcc
    16561 agctccgttt ttcccaatca gcccaacaat ctcgccctta ttgatctcta aactcacatc
    16621 ttttagtgag taaaattcgt ttcctgcaaa ctgttttgat aaatgattga ttttaagcat
    16681 aagtaactcc tttttctttc tgacgacttg tttgttgaat aaagtatgta gcaatagcta
    16741 caccataata cactacagca acaaatagta tttgtgatgg agctaacaga ttactagcta
    16801 cctcgacact catgacatcg tctgaaactg tattcagttg agaaaactga gcccactgac
    16861 cattactact cgtaatgtag gcaaaagcag cataaaatat cattgacatt gttggaaaaa
    16921 tgcctgctga ccatgctttc caagaattat cctttagcaa tcctaaacag attgaattga
    16981 caatgggtgg tagcaaatag gtcaacaata acaaaatagg aaccgaaagg cttaacagca
    17041 gttgcgaact tacaactgtc aaacaagtat acaataaact caatagtaga ataaatatca
    17101 tcataaccct cacaattaaa atggaaatct cgaaaccaac agattgtaga tccaatgtcc
    17161 tccgatagca tactgcaatc caaaacgtgt agctaacaca gagaacaata aacctagtgg
    17221 agttcgaatg attagattat ccaacaatgg ataattcata tgtagcaaaa ctccaaacaa
    17281 gagagaagtg accaacatag ctagccaagt agggtattca cttttcagaa tacggtagat
    17341 aagataacgg aacaagaatt cttcacaagt cgcaataata aattgattga catactcatc
    17401 actttgaata caccagaaat tgatcaggaa aattgcaata gccagcaaaa caaaactttt
    17461 ccaactgaac tttacttccg atagctcctg gcaaagttga taacgcttgg ttaacataga
    17521 tggaaataga ataaaaccta ctggcattcc gaccataatg gaaaataggt aagccacctg
    17581 taaaaattga ctgggactaa acaaaaccaa agatgttgcc agaagatatg aaattgacgc
    17641 taaaataatc aaagcaattg ataaggcaac tggtctaata tagcgtatta ccataaaaca
    17701 cctctaaaat tctagctttt caaagttcga acggcctttg agataggtag caacaatata
    17761 taatgttgtt atagctgcta ccaaggacca tgcaagcagt ggctgattaa agctcatctg
    17821 agcatagcca ttcggtacga cataacgaac tccaatgaga aatggtgcag ttgttgtaaa
    17881 cagcgaccaa aaaactcctg ctaaaactgc ctgtaaggat ttagctttga tagaaaccat
    17941 ggcaaccaat agaataccga taacattgat tgaaattgtt gcaaaaatag ataggaaggt
    18001 ggactgtaca ttattccaat catccgatac tacttttcca gaagcattca agaagccaaa
    18061 ataagcaatc gcacttgtca gcattgttag aaggacataa gaggcataca tcgtcactaa
    18121 actaatgatt tttgcattaa aaagtcgagt tctattc
//
LOCUS       DAZQCJ010000033        17529 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000033 DAZQCJ010000000
VERSION     DAZQCJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17529
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.033"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..1422
                     /locus_tag="ACHU66_001032"
     CDS             <1..1422
                     /locus_tag="ACHU66_001032"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR3389324.1"
                     /translation="YADWTPEYIEKIKKNTENSPWRTNFHIETPHGLLNDPNGFSYFN
                     GKWTLFYQYFPFGAAHGLKSWVHTESTDLVHFEKTGTVMYPDTPLDSHGAYSGSAMEF
                     GDKLFLFYTGNVRDENWVRHPYQIGALMDKDGKIEKIDKVLIEQPEDTTDHFRDPQIF
                     NYKGQYYAIVGGQNLDKKGIVKLYKAENNDYLNWSYVGDLDFANDMTAYMMECPNIAF
                     VGEQPILLYCPQGLDKEVLDYSNIYPNMYKIGQSFDPETASIVEPSELINLDYGFECY
                     ATQAFNAPDGRTLSVSWLALPDVEYPTDAYDYQGCLSLVKELTIKDGKLYQYPVDAIT
                     SLRKEAQPFSAQKETNNVYELELDFSAGTKHEIVLFANEAEKGLVLSVDTEQGQITLD
                     RGNCGQPFALDFGTSRSCEISPGALTANIFIDKSVFEIFINKGEKVFSGRVFPDADQS
                     GIVITTGNPTGTYYHLDYGRKTN"
     gene            1403..2368
                     /locus_tag="ACHU66_001033"
     CDS             1403..2368
                     /locus_tag="ACHU66_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR3389325.1"
                     /translation="MVAKLTDVAQLAGVSPTTVSRVINKKGYLSEKTIRNVEEAMREL
                     GYKPNNLARSLQGKSAKLVGLIFPTINNIFYSELISHLEKELFDRGYKTIICNSQHES
                     DKEREYLEMLAANQVDGIISGSHNLGIQDYDRVVAPIIAFDRNLSPSIPIVSSDNFAG
                     GVLAAQTLQKAGCHNPLMITGNDDSNSPTGLRQVGFTSILNKAEVFHISSDFSPVRKE
                     MEIRAILEKQKPDGIFVSGDATAMLVWNVIRSMKLSIPEDIKLIGYDGTSFIENYYPQ
                     LTTIKQPLTEIARLLVELLVDKIEGKKLTKTDYILPISLLAGASI"
     gene            complement(2447..2866)
                     /gene="nusB"
                     /locus_tag="ACHU66_001034"
     CDS             complement(2447..2866)
                     /gene="nusB"
                     /locus_tag="ACHU66_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR3389326.1"
                     /translation="MKNNRHALRKCALQAIVSLEFGQEPVQAAQFSYLYDREEEQEGV
                     EIPLFLLNLVNGVADYRDDLDKELSSRLKAGWTLDRLTLIDKNIMRLGLFEILHFEET
                     PDRVAVNEAIELAKEFSDESSAKFVNGVLSQFIKEEA"
     gene            complement(2856..3248)
                     /locus_tag="ACHU66_001035"
     CDS             complement(2856..3248)
                     /locus_tag="ACHU66_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFR3389327.1"
                     /translation="MTTEYQGEIVIAPRVLEVITGIAAAKVDGVHSLQNKRVADSLSK
                     TSLNKGVYLETDEEGRVTADIYVYLEYGVNVPAVSMDIQRAVKTAVYNYAEVQVAAVN
                     IHVSGIVPDKTPKPALKDLFGEDFLDEE"
     gene            complement(3276..3833)
                     /gene="efp"
                     /locus_tag="ACHU66_001036"
     CDS             complement(3276..3833)
                     /gene="efp"
                     /locus_tag="ACHU66_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR3389328.1"
                     /translation="MIEASKLRAGMTFEAEGKLIKVLDASHHKPGKGNTIMRMKLRDV
                     RTGSTFDTTYRPDEKFEQAIIETVPAQYLYQMDDTAYFMNTETYDQYEIPVATIKEEL
                     LYILENSDVKIQFYGTEVIGVTVPTTVELVVAETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(3897..4958)
                     /locus_tag="ACHU66_001037"
     CDS             complement(3897..4958)
                     /locus_tag="ACHU66_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFR3389329.1"
                     /translation="MQARVEKFEAKLAQSDVDGILVTGQNNIYYLTGFWGTEATVFIS
                     GKRRLFVTDSRYTLIAKASVKGFDIIESRFALEEIAKVIKEDGLEKIGFDSEVTYGFY
                     QSLTSIFEGYQLVAMSNFIEDLRMIKDEKEIATIRRACQISDQAFIDVLDFIKPGQTT
                     EMDVNHFLDHRMRQLGAEGASFEFIVASGYRSAMPHGRASEKVIQAGETLTLDFGCYY
                     QHYVSDMTRTIHIGHVTDQEREIYDVVLRANKALIEQAKEGVTYREFDAIPREIINAA
                     GYGANFTHGIGHGIGLDIHEYPYFGKSDETIKAGMVLTDEPGIYLDDKYGVRIEDDLL
                     ITENGCEVLTLAPKELIVI"
     gene            complement(5057..7882)
                     /gene="uvrA"
                     /locus_tag="ACHU66_001038"
     CDS             complement(5057..7882)
                     /gene="uvrA"
                     /locus_tag="ACHU66_001038"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR3389330.1"
                     /translation="MQENIVIHGARAHNLKNIDVTIPREKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMDKPDVDSIEGLSPAISIDQKTTSRNPRSTVGTA
                     TEINDYLRLLYARVGVPYCINGHGAIAASSVEQIVDEVLELPERQRLQILAPIVRKKK
                     GQHKTIFEKVQKDGYVRVRVNGDVYDVSEVPELSKSKAHNIEVVVDRIVIKEGIRSRL
                     FDSIEAALRIADGYVIIDTMDDKELLFSEYYACPVCGFTVPELEPRLFSFNAPFGSCS
                     DCDGLGMKLEVDTDLIVPDASKTLRDGALAPWNPISSNYYPQMLEQAMNHFGVDMDKP
                     FEELTEEEKNLIFNGSDGKEFHFHYENEFGGVRDIDIPFEGLITNINRRYRETNSDYT
                     RTVMKTYMNELTCGTCHGYRLNDQALSVKVGGEQGLHIGQLSDLSVADHLEVIEHLTL
                     SENEATIATPIVKEIKDRLSFLNNVGLNYLTLSRAAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADWLIDIGPGA
                     GVFGGEIVASGTPAQVAKNKKSITGQYLSGKREIPVPLERRVGNGRFLEVTGAKENNL
                     QDVTVRFPLGKFVAVTGVSGSGKSTLVNSILKKAIAQKLNRNSDKPGKFKSISGIEHL
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVFVPCEVCHGHRYNSETLEVHYKEKNIAQVLDMTVNDAVE
                     FFKHIPKIERKLRTIQDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHTEDIAQLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDMGPEGGVGGGT
                     VVATGTPEEVASNPASFTGQYLKMKLK"
     gene            8721..9665
                     /locus_tag="ACHU66_001039"
     CDS             8721..9665
                     /locus_tag="ACHU66_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFR3389331.1"
                     /translation="MKQIFLSTMTDLEEIQTFEPGAWINLVNPSQSESMEVAEHYKID
                     IADLRAPLDAEESSRITVEDDYTLILVDVPILEERNNKTYYITIPLGIILTDDAIITT
                     CLEPLPLFEQFIHRRLRNFFTFMKSRFVFQILYRNAQLYLSALRTIDRKSEEIEKQLH
                     EATRNEELIVLMELEKTIVYFKASLKTNERLVKKLASSSRLLRKYEEDEDLLEDTLVE
                     TQQAIEMADIYGSILNSMTHAFASIISNNQNTIMKTLALVTIVLSIPTMVFSAYGMNF
                     KDNIIPFNDIPYAFWWIILFAFAISLSLTFYFIRKRWF"
     gene            9681..10337
                     /locus_tag="ACHU66_001040"
     CDS             9681..10337
                     /locus_tag="ACHU66_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFR3389332.1"
                     /translation="MSFTEINGLTKKNQEFIHIATNQLIKDGKSDSEIKELLEEILPT
                     IIEKQKTGVTARNLYGAPSEWAASKTVSEQEKKDQVEYNENPWLMWLDSSLFMLAIIA
                     GINGLMNLFGQGAQYGLLTLFVIGFGVGAGMYLMYHFVYREQIKTGQRPKLLKAIAFL
                     GLATLAWSVVFILAALIPAAFNPVLPPLVTILIGAAAFGARYLLKKKYNIRNAMSPVQ
                     "
     gene            complement(10780..11019)
                     /gene="rpsR"
                     /locus_tag="ACHU66_001041"
     CDS             complement(10780..11019)
                     /gene="rpsR"
                     /locus_tag="ACHU66_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR3389333.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFISERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALLPFVNED"
     gene            complement(11052..11546)
                     /locus_tag="ACHU66_001042"
     CDS             complement(11052..11546)
                     /locus_tag="ACHU66_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR3389334.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFTLAVNRNFKNQNGER
                     EADFINVVIWRQQAENLANWAKKGALIGVTGRIQTRSYDNQQGQRVYVTEVVAESFQL
                     LESRTAREGQGGGYSAGNSFAGGNDYNSPYQAPAQSTPNFAREESPFGASNPMDISDD
                     DLPF"
     gene            complement(11558..11848)
                     /gene="rpsF"
                     /locus_tag="ACHU66_001043"
     CDS             complement(11558..11848)
                     /gene="rpsF"
                     /locus_tag="ACHU66_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR3389335.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATIVESKAWE
                     KRRLAYEIKDFREGLYHIVNVEANNDEALKEFDRLSKINGDILRHMIVKLDA"
     gene            complement(12020..13009)
                     /locus_tag="ACHU66_001044"
     CDS             complement(12020..13009)
                     /locus_tag="ACHU66_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFR3389336.1"
                     /translation="MKYIVNNSNDPAYNIALEAYAFKELTDIDEIFILWINEPAIIIG
                     KHQNAIEEINKEFTDEKGIHVVRRLSGGGAVYHDLNNLNYTIISNKADEGAFDFKTFS
                     KPVIDTLATLGVEANFTGRNDLEIDGKKICGNAQAYAKGRMMHHGCLLFDVDMSVLAS
                     ALKVSKDKIESKGVKSVRARVTNINNELPEKMTVLEFKDAILNQMKQEYPDMDEYVLS
                     EGDLVRIQEIRDTQFATWDWTYGQTPEYTVERSVRYPAGKITTYIKAEKSVIESIKIY
                     GDFFGIGDVSDIEDLLVGTRYEYADVLAKLQEIDTTHYFSRMTTEEVAKAIVA"
     gene            complement(13093..14532)
                     /locus_tag="ACHU66_001045"
     CDS             complement(13093..14532)
                     /locus_tag="ACHU66_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR3389337.1"
                     /translation="MTVTKVFPKGFLWGGATAANQCEGAYDVDGRGLANVDVVPIGQD
                     RAAIITGRKKMFDFEDGYFYPAKESIDMYHHFKEDIALFGEMGFKTYRLSIAWSRIFP
                     KGDELEPNEKGLQFYEDLFKECHKYGIEPLVTITHFDCPMHLIENYGGWRNRKMLEFY
                     ERLCRTLFTRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENVEQVKYQAAHHELV
                     ASAIATKLAHEIDPENKVGCMLAAGQYYPHTCHPRDVWEALQEDRENYFFIDVQARGY
                     YPNYAKKKWERAGIEIEMTNQDLALLKEHTVDFISFSYYSSRVASGDPVEKEKTAGNI
                     FASIKNPYLDASEWGWQIDPLGFRITINSIWDRYQKPLFVVENGLGAVDTPDENGYVE
                     DDYRIDYLRQHVLAMRDAIVEDGVELMGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDN
                     EGNGTLKRSKKKSFEWYKKVIATNGADVG"
     gene            complement(14529..15146)
                     /locus_tag="ACHU66_001046"
     CDS             complement(14529..15146)
                     /locus_tag="ACHU66_001046"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFR3389338.1"
                     /translation="MNYLYLMRHGQTRFNQQGRIQGACDSPLTELGREQALAAHQYFQ
                     EQGIEFDKIYSSTQERACDTAELVTGRTDYIRLKGLKEQDFGAFEGQQEYLNPPLQGD
                     IGYGDYFVTFGGESYQDVRQRMVETIGGIMEEADNQSVLVVSHGAAIAQFFRQVLTDY
                     PQVRMRNCAILTFTYEDGHYDLQSIVDPVNKENLYQKEGKEKEKK"
     gene            complement(15158..15760)
                     /locus_tag="ACHU66_001047"
     CDS             complement(15158..15760)
                     /locus_tag="ACHU66_001047"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFR3389339.1"
                     /translation="MTKTIYLMRHGETLFNTQKRVQGWSDSPLTERGIAQAQAVGQYF
                     KEQGIVFTSAYSSTQERATDTLKLVTDAPYQQLKGLKEMNFGIFEAREEELLPKHRPG
                     ARSFEDLLVPFGGEDIRAVGSRVKEAILSVAESDPADPILMVSHGAAIWGLVQVLEVT
                     FPPGVFLPNCAICQLTVENEQIRFEKLIDSAHDFKVYDFK"
     gene            complement(15769..17235)
                     /locus_tag="ACHU66_001048"
     CDS             complement(15769..17235)
                     /locus_tag="ACHU66_001048"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFR3389340.1"
                     /translation="MTKQFPKGFLWGGATAANQYEGGWNLGGRGPATSDTYIAVDPDK
                     RKDMSHFGKPVSRADVEFALADQEGLYPKRWGSDFYHRYKEDIALFAEMGFKTFRLSI
                     AWSRIFPKGDELEPNEEGLAFYDAVFDELNKYGIEPLVTLSHYEFPLHLALEYGGWKN
                     RKVIEFFVRYAETVFKRYQGKVKYWLTFNEINILGMVGYLSGGLLFEDGKNDLEAMYQ
                     AVHHQFIASSLATKAAHEIDPENKVGCMLARMENYAATCNPDDVLAALKKDQENLFYT
                     DVQVRGAYPSYMKRFFKENNIQVVFEPGDEAILKQYPVDFMSFSYYMTSITRALPDKD
                     KATAGNLILGEANPYLEASDWGWQIDPVGLRITLNKLYDRYQVPLFIVENGLGALDKV
                     EADGSIEDGYRIAYLEKHIQQMYEAIEDGVELMGYTPWGCIDLVSASTSEMSKRYGFI
                     YVDADDQGKGSFDRSRKASFYWYKDVIASNGANVLTEK"
BASE COUNT         4913 a         4105 c         3548 g         4963 t
ORIGIN      
        1 tatgctgact ggacacccga atatattgaa aaaataaaga agaatacaga gaattctcct
       61 tggaggacaa atttccatat tgaaacacca catggactgt taaatgatcc aaatggattt
      121 tcttatttta atggaaagtg gactcttttt taccaatact tcccattcgg tgctgcgcac
      181 ggattaaaat cttgggtcca tacagaatca acggatttgg tccattttga aaaaactggt
      241 acggttatgt acccagatac accacttgat agccacggtg cctactctgg atctgccatg
      301 gaatttggcg acaagctctt cctcttctat actggaaatg tccgcgatga aaattgggtt
      361 cgccatccat accaaatcgg tgccttgatg gataaagatg gtaagattga aaagattgat
      421 aaagtcttga ttgagcaacc agaagatacc acggaccatt tccgtgatcc acaaattttc
      481 aactataaag ggcaatatta cgctattgtc ggtggacaaa atctggataa aaagggaatt
      541 gtcaaacttt ataaagctga aaataacgac tacctcaact ggtcctatgt tggtgacctt
      601 gattttgcca atgatatgac cgcttatatg atggaatgcc caaatattgc gttcgtcggt
      661 gagcaaccca tccttctcta ctgtccacaa ggcttggaca aagaggtatt ggactatagc
      721 aatatctatc caaatatgta caagattggt cagagtttcg atcctgaaac agcaagcatt
      781 gtcgaaccga gcgaattgat caatctggac tatggttttg agtgctatgc aacccaggcc
      841 tttaatgcac cagacggtcg aactctatct gttagctggt tggcacttcc agatgtggaa
      901 tacccaaccg atgcttatga ctaccaaggc tgtctgtcat tggttaaaga attaaccatc
      961 aaggacggta agctctacca gtatccagtt gatgcgatca ccagtcttcg taaagaagct
     1021 cagccatttt cagcccaaaa agaaactaat aatgtttacg aattggaact tgatttttca
     1081 gcgggcacga agcacgaaat cgtattgttc gcaaatgaag cagaaaaagg cttggtcttg
     1141 tctgtggaca ctgaacaagg ccagattaca ttggaccgcg gcaactgtgg tcagccattc
     1201 gcactcgatt tcggtacaag tcgctcttgc gagatttctc caggagctct cacagccaat
     1261 atcttcatcg acaaatctgt ctttgaaatc tttatcaata aaggagagaa agtattttct
     1321 ggtcgtgtct tccctgatgc ggatcagtct ggtatcgtca ttacgactgg taatccgacc
     1381 ggaacctact accatttaga ttatggtcgc aaaactaact gatgtcgcac agttagctgg
     1441 ggtcagccca acaactgtct cgcgcgttat caataaaaaa ggatacctat ctgaaaaaac
     1501 cattcgcaat gtcgaagagg ctatgcgtga actgggctac aaacctaata atttagctcg
     1561 tagtttgcaa ggtaaatctg caaaattagt tggtctcatc ttcccaacaa taaacaatat
     1621 tttttattct gaattgatta gccatttaga aaaagagctt tttgatcgag gctataaaac
     1681 cattatttgc aatagccagc acgagtccga taaggaacgt gagtatctag aaatgttagc
     1741 agctaaccaa gtagatggga ttatctcggg cagtcacaat ctcggtatcc aagactacga
     1801 ccgtgtcgta gctccaatta tcgcttttga ccgcaacctc tctccttcca tccccattgt
     1861 ctcttccgat aattttgctg gcggggtatt agctgcccaa accttacaaa aagcgggatg
     1921 tcacaatcca ttaatgatta cggggaacga tgattccaat tcacctaccg gccttcgcca
     1981 agtaggtttc acatctattt tgaacaaagc cgaagttttt catatctcca gtgatttctc
     2041 acctgtgcgc aaggagatgg aaatccgagc cattcttgaa aagcaaaaac cagatggtat
     2101 ctttgtatct ggcgatgcaa cggctatgct ggtatggaac gtcattcgtt caatgaaact
     2161 gtctattcca gaagatatta aattgattgg ttatgatgga accagtttca tcgaaaacta
     2221 ctatcctcaa ctgacaacca tcaagcaacc attaaccgaa atagctcgcc tgctggttga
     2281 actattagtt gataaaatcg aaggaaaaaa actgacaaaa acagactata tcctaccgat
     2341 tagcctacta gcaggagcta gtatataaaa aaactgtacc taataaaaat taggtgcagt
     2401 ttttttgtga tacattctct tgtttctcaa gtaaaaaaga agctcatcag gcttcttctt
     2461 taataaactg gctcaaaact ccattgacaa acttagctga agattcatca gaaaattctt
     2521 tagccaattc aatagcctcg ttaacagcaa cgcggtctgg cgtttcttcg aaatgtaaga
     2581 tttcaaatag gcctagtcgc atgatattct tgtcaatcaa ggttaaacgg tctaaggtcc
     2641 atcctgcttt taatcggcta gataactcct tatccaagtc atcacgatag tctgctacac
     2701 cattgacaag gttaaggaga aagagcggaa tttcaactcc ttcttgctct tcctctctat
     2761 cataaagata agaaaactga gctgcctgaa caggttcttg accaaattca agtgatacga
     2821 ttgcctgcaa agcacactta cgcagggcat gtctattatt cttcatcaag gaaatcctct
     2881 ccgaataaat ctttcaatgc tggttttggt gttttatcag gaacaatgcc gctgacatgg
     2941 atattaaccg cagccacttg aacttctgca tagttataaa ctgctgtctt gactgcacgt
     3001 tggatgtcca ttgatacggc aggaacattt acaccatatt ctagatagac gtaaatatcc
     3061 gcagtcactc gaccttcctc gtcagtttca agataaacac ctttatttaa cgatgttttt
     3121 gacaaactgt ctgctacacg cttattttga agggaatgca ctccatcaac tttcgctgca
     3181 gcaatacctg taattacttc aaggacgcga ggtgcgataa caatctcgcc ttgatattct
     3241 gttgtcatag gctctccttt cttaagagga aacgattaag cacgtgatac gtaagttcct
     3301 tcagctgtgt tgataacgag tttttgacct gcttcgataa agtctggtac gttgactaca
     3361 agacctgttt ccatcgtagc tggtttacct gaacctgtaa cagtcgcacc tttgatagat
     3421 ggttgtgttt ctgcaacgac caattcaaca gttgttggta cagtaacacc gatcacttct
     3481 gttccgtaga actggatctt cacatcggaa ttctccaaga tatagagcaa ttcttctttg
     3541 atagtcgcta ctggaatttc atactgatcg taagtctcag tattcatgaa ataagcagtg
     3601 tcatccattt ggtaaaggta ctgtgctgga actgtttcga taatagcttg ttcaaatttt
     3661 tcatctggac gataagttgt atcgaaggtt gaacctgtac gtacatcacg caatttcata
     3721 cgcatgatgg tgtttccttt acctggtttg tggtggcttg catccaaaac tttaatcaat
     3781 ttaccttcag cttcgaaggt catacctgct ctaagtttac ttgcttcaat cattgtattg
     3841 aatacctctt caaattttat ttattcgctt tattctacca caaactgtca aatttctcaa
     3901 atgacaatca attccttggg agctaatgtc aagacttcac aaccgttttc cgtaatcaat
     3961 aggtcatctt cgatacggac accatatttg tcatccaaat agataccagg ctcatctgtc
     4021 aagaccatac ctgccttgat agtctcgtca gattttccaa aatacggata ttcatgaata
     4081 tccaagccga taccgtgacc gatgccgtgt gtaaagttgg ctccgtaacc cgccgcattg
     4141 ataatctcac gaggaatagc gtcaaactcg cggtaggtga caccttcctt ggcctgctca
     4201 atcaaagcct tattggcacg caagaccaca tcgtaaatct cacgctcttg atctgtcaca
     4261 tgaccgatat ggatggtccg tgtcatatcg ctgacataat gctggtaata gcaaccaaaa
     4321 tccagcgtca aggtctcacc agcttggata accttttcag acgctcttcc atgaggcata
     4381 gcagagcggt agccagatgc cacgatgaac tcaaatgatg ccccttctgc accgagttga
     4441 cgcatgcggt ggtcaaggaa atgattgacg tccatctcag tcgtctgacc tggtttgata
     4501 aagtcaagaa catcaataaa agcctgatcc gaaatttgac atgcacggcg aatggtcgca
     4561 atttctttct catctttaat catgcgcaag tcttcgataa agtttgacat agcaaccagt
     4621 tgataaccct caaaaatgct ggtcagactt tgatagaaac cataggtcac ctcgctatca
     4681 aaaccaattt tttcaagccc atcttcctta atcacctttg caatctcttc aagggcaaaa
     4741 cggctctcga taatatcaaa accttttact gatgccttgg ctatcaaggt ataacgcgaa
     4801 tcagtcacaa acaagcgacg cttgccactg atgaagacag tcgcctctgt cccccaaaaa
     4861 cctgtcaggt agtaaatatt gttttgacca gtcaccaaga ttccatccac atcagattgg
     4921 gctaattttg cctcaaattt ttccacacgc gcttgcataa gctacctcca aagattttgt
     4981 aactattata gcagaaatac agacaaatac caattcttcc tagcagatag agaaaaaaag
     5041 ccgcagaggc ggctttttat ttcaacttca ttttcagata ctggcctgta aagctggctg
     5101 ggttgctagc tacttcttct ggcgttcctg ttgcaacaac ggtaccacca ccgacaccgc
     5161 cttctggtcc catgtcgata atgtggtcgg ctgtcttgat gacatccaga ttgtgctcga
     5221 taacaaggac agtattgccg tcatcaacaa agcgggccaa gaccttgagc aattgggcaa
     5281 tatcctcagt atgtagacct gtcgttggct catccaagat atagagggac ttaccagtcg
     5341 accgcttgtg aagttccgat gccaatttca tccgctgggc ctccccacct gatagggttg
     5401 tcgcaggttg tccaagggta acatagccca agcctacatc ttgaatggtg cgaagtttcc
     5461 gctcaatttt cggaatatgt ttgaagaact cgaccgcatc attgaccgtc atatcaagga
     5521 cctgggcgat atttttctcc ttgtaatgta cttccaaggt ttcagaattg tagcggtgtc
     5581 catgacagac ttcacaaggt acaaagacat ctggcaggaa gtgcatttca atcttgatga
     5641 taccatcacc agaacaagcc tcacaacgac cacctttgac gttaaaggag aaccgtccct
     5701 tcttgtagcc acggatttta gcctcattag tctgagcaaa gaggtcccga atatcgtcaa
     5761 aaacacctgt ataagtagct gggttggaac gcggtgttcg tccaatcggg ctttgatcaa
     5821 tgtcaatcag gcggtccaag tgttcgatgc ctgaaatgga cttgaacttg cctggcttgt
     5881 ccgaattgcg attgagtttc tgagcaatag cttttttcaa aatggaattg accagggttg
     5941 atttgcctga gccagacacc cctgtcaccg ctacaaattt tccgagcggg aaacgaacag
     6001 tcacatcttg caggttattt tccttggcac ctgttacctc caggaaacga ccattgccaa
     6061 cacgacgttc tagaggaact ggaatttcac gtttgccaga caggtactgg cctgtaatgg
     6121 attttttatt tttagctact tgggcaggcg ttccagaagc cacgatttct ccaccaaaga
     6181 cacccgcacc tggaccgatg tcaatcaacc agtccgcctc acgcatggtg tcctcatcat
     6241 gctccaccac aatcaaggta ttgcctaaat cacgcatctt tttcaagctg gcaatgagac
     6301 gatcattgtc ccgctggtgc agaccaatcg acggttcatc cagaatatag aggacaccag
     6361 acaggttaga gccaatctga gtcgccagac gaatccgctg gctctcacca ccagaaaggg
     6421 tacctgctgc tcgtgaaaga gtcagatagt tgagaccaac gttatttagg aaggaaaggc
     6481 ggtccttgat ttccttgaca atgggtgttg caatggtggc ttcattttca gacaaggtca
     6541 gatgttcaat gacttccaga tgatccgcta cggacaaatc agacaactgc ccgatatgaa
     6601 gtccctgctc gccaccaact ttgacagaca gggcttggtc attgaggcga tagccgtggc
     6661 aggttccaca ggtcaattca ttcatgtaag tcttcatgac tgtccgtgtg taatcactat
     6721 tggtttcacg atagcgacgg ttgatattgg taatcaaacc ttcaaaggga atatcgatat
     6781 cacgcacccc accgaactca ttttcataat ggaaatgaaa ttctttcccg tcagaaccgt
     6841 tgaaaatcag gttcttctcc tcttccgtca attcctcaaa aggcttgtcc atgtccacac
     6901 caaaatggtt catggcctgc tccaacatct gagggtagta gttagaagaa ataggattcc
     6961 acggagccaa agccccgtca cgcaaggtct tgctggcatc tgggacaatc aaatccgtat
     7021 ccacttccag cttcatgccc aaaccgtcac agtcgctaca agacccaaaa ggagcattga
     7081 aagagaagag gcgaggctcc aactcaggca ctgtaaagcc gcaaactgga caggcatagt
     7141 actcagagaa aagcagctcc ttgtcatcca tggtatcaat gatgacataa ccgtccgcaa
     7201 tccgcagggc cgcttcgata gaatcaaaaa gtcgcgagcg aatgccctct ttgatcacaa
     7261 tccgatcgac cacgacttca atattatgag ccttgctctt ggacaattct ggtacttccg
     7321 acacatcata gacatcacca ttaacacgaa cccgaacata gccgtccttc tgcaccttct
     7381 caaaaatggt cttgtgttgc ccctttttct tacgaacaat cggagctaga atctgcaaac
     7441 gctggcgttc tggcaattcc aagacctcat cgacgatctg ctccaccgaa gaagccgcaa
     7501 tcgccccgtg cccattgata cagtacggca ccccgacccg agcatagagc aggcggagat
     7561 agtcattgat ttccgtcgct gtaccgactg tcgaacgcgg attccgcgac gtcgtttttt
     7621 ggtcaatgga aatagcaggg ctgagcccct ctatggaatc cacatctggc ttgtccatgt
     7681 tgcccaagaa ctgacgagca taggcagaca aagactccac atagcgacgt tggccttcgg
     7741 catacagagt atcaaaagcc aaactcgact taccagaccc cgacaaacca gtcaccacca
     7801 ccagcttctc acgcggaatg gtcacatcaa tatttttcaa attatgggca cgcgccccat
     7861 gaatcacaat attttcttgc ataatttaag atacagagcc cgtaaaacgc aaggtgaaaa
     7921 taggaaattt gacgaagagc gttaacgctc taggagaatt tgtctttttc acacagcgtt
     7981 tagggcgtgt tcaattcctt tctagatata agtgcgttaa gaaagcgaga agaaaatagg
     8041 aaaatgtgac gtcacatcag tgacaaaaca ttttgtcttt ttctcgacgc ttttagcacg
     8101 cattcgaatc ctttctaaga tacaaagggc gtcaaatgca tagtcaaaat aggcggtaag
     8161 tccgaaatct gtgatttcga agacgcaccc ccgtcaagac aactagtgct ttcacaggtc
     8221 gaaaaccgaa gaaagcacag atgtctacga cgcagaagct aattccccat actcatacat
     8281 gaagaaacca gcaatttttg acactgcatt tagcccgtgt tcatttcctt tctaaagata
     8341 caaagaccgt aaaacgcacc gtcaaaatag gcggtaagtc cgaaatctgt gattccgaag
     8401 acgcaccccc gtcaagacaa ctagtgcttt cacaggttga taaccgaaga aagcacagat
     8461 gtctacgacg caaaagccag tttccatgct gaaacatgaa gaaaccagca atttttgaca
     8521 ccgcatttag cccgtgttca attcctttct taagaggcta taagctatgg tatacggaac
     8581 aaggaaaagt ccgtacaact tattcgctta aatgtctaca tattatatca catttctata
     8641 taaaaccttt ccgaaactgc acattttttg gtataataga ggttgcgtta actcaacaaa
     8701 gataaaagga gggcaaagat atgaaacaaa tttttctatc aaccatgact gatttagaag
     8761 aaatccaaac ctttgaacca ggggcttgga ttaatttggt caacccctcc cagagcgagt
     8821 ctatggaagt ggctgagcac tataaaattg acatcgctga cctgcgagca ccgctcgatg
     8881 cggaagaatc ctctcgtatc accgtcgaag atgactatac gctcatcctg gtcgacgtcc
     8941 caattcttga agaacgaaac aacaagacat attatattac tattcctttg ggaattattc
     9001 taacagatga tgcgattatc acaacttgtt tggaaccatt gccactgttt gagcagttta
     9061 tccatcgtcg tctacggaat ttcttcactt tcatgaagtc tcgtttcgtt ttccaaattc
     9121 tctaccgcaa tgctcaactc tacttgtcag ccctacggac cattgacaga aagagcgaag
     9181 agattgagaa gcaactccat gaggccactc gaaatgaaga attaattgta ctcatggaat
     9241 tggaaaaaac catcgtctac ttcaaggctt ccctcaaaac caatgagcgt ttggtcaaaa
     9301 aacttgctag ttcctcacgt ctactccgta agtatgaaga ggatgaggat ttgcttgaag
     9361 ataccttggt cgaaacccaa caggccattg agatggcaga catctatggt tccatcttga
     9421 actctatgac ccatgccttt gcttctatca tttctaacaa ccagaatacc atcatgaaaa
     9481 ccttggcatt agtgacaatc gttctctcta tcccaaccat ggtcttctct gcatacggta
     9541 tgaactttaa ggacaacatc attcctttta acgatattcc ctatgctttt tggtggatta
     9601 tcctcttcgc atttgcaatc agcctatcac tcaccttcta cttcatccgt aagagatggt
     9661 tttaacctaa ggagattaac atgtcattta cagaaatcaa cggattaaca aaaaagaacc
     9721 aagaatttat ccatattgcg accaaccaac tgattaaaga tggaaagtct gatagcgaaa
     9781 tcaaagaact cttggaagaa attttaccaa ctatcattga aaagcaaaaa actggcgtga
     9841 ctgcccgcaa tctctatgga gcaccaagtg agtgggctgc tagtaagaca gtttctgaac
     9901 aggaaaagaa agaccaagtg gaatacaatg aaaatccatg gttgatgtgg ctagattcta
     9961 gtcttttcat gcttgcaatt attgctggta tcaatggttt gatgaatctt tttggtcaag
    10021 gtgcacagta tgggctatta accttatttg tcatcggttt cggtgttggt gcagggatgt
    10081 acttgatgta ccatttcgtt tacagagaac aaatcaaaac tgggcaacgt cctaaattgc
    10141 tgaaagctat cgcttttctt ggacttgcta cgctggcttg gtcagtcgtt ttcattctgg
    10201 cagcactgat tccggccgct tttaacccag ttctccctcc gcttgttact attcttatcg
    10261 gagctgctgc tttcggtgca cgctacctct tgaaaaagaa atataacatt cgcaatgcca
    10321 tgtcaccagt tcaataataa aacaaagcga ttggaatatt ccagtcgctt ttgtttatct
    10381 atacagtcca gctatcaaat cgcccgagaa ctttatccgc tgtcacttgt ctatgtaatc
    10441 tgctgaaaca gtctatcccc agactgtgcc tcgctttcta acttcttggc tcgggttgaa
    10501 acagtccatt caagggctta attcgctttc ttaatttcga gttcatgaaa aaatggtcca
    10561 ctctcgccgt tgcgcctttc cagtttcttg ggcgcaagct aaaaagaccc gtcggggtct
    10621 caattcgctt tcttattttc atgctcatga aaaaacagtc cattcaagga cttaattcgc
    10681 tttcttaatt tcgagctcat gaaaaaacag tccattcaag gacttaattc gctttcttaa
    10741 tttcgagctc atgaaaaaat ggtccactgg accatttttt taatcttcgt ttacgaatgg
    10801 taaaagagcc ataacgcgag cacgtttgat agctgttgtt actttacgtt ggtttttagc
    10861 tgaagttcca gttacacggc gtggaaggat ttttccacgt tcagaaatga aacggctaag
    10921 aagctcagta tctttgtaat caacatattc aattttgtta gctgcgatat agtcaacttt
    10981 tttacggcgt ttgaaaccgc cacgacgttg ttgagccatg tgtgttctcc tttatattat
    11041 taatttttga cctagaatgg taggtcatcg tctgatatat ccattggatt acttgctcca
    11101 aatggacttt cttctcgagc gaagtttggt gtagattgtg caggcgcttg ataaggtgag
    11161 ttgtaatcat ttcctccagc aaacgagttg ccagctgaat agcctccacc ttgaccttca
    11221 cgggcagtac ggctttccaa gagttggaaa ctttctgcaa ctacctcagt aacgtagaca
    11281 cgttgccctt gctgattgtc atagctacgt gtctggatac gacctgtaac accaatcaga
    11341 gcacctttct tagcccaatt cgccaaattc tcagcttgtt gacgccaaat gactacgttg
    11401 ataaagtccg cttcacgctc accgttttga tttttaaaat tgcggttaac cgccaaagta
    11461 aaagtcgcaa cagcttgatt agacggagta taacgaagtt ctgcatcacg ggtcatacga
    11521 ccaaccaata ctacattatt aatcatacaa caccttctta cgcgtcaagt ttgacaatca
    11581 tgtgacgaag aatgtcgccg ttgatttttg acaaacggtc aaactcttta agagcttcat
    11641 cgttgttagc ttcaacgtta acgatgtggt acaaaccttc gcggaaatct ttgatttcgt
    11701 atgcaaggcg acgtttttcc catgcttttg attcaacgat agttgcaccg ttgtcagtca
    11761 agatagagtc aaagcgtgct accaaagcgt ttttagcttc ttcttcaatg tttggacgaa
    11821 taatataaag aatttcgtat ttagccattg atatgttcct ccttttggtc taatgactca
    11881 tggtctagcc acgagtaagt gagggatact cacagaaaat aattatacca taatatagaa
    11941 aatgtgcaag tgaaaacagt ttgccgaaaa cattttcaca aaaaataaag actgcccaaa
    12001 caaagggcag ccctatggat taagctacaa tcgcttttgc tacttcttct gttgtcatgc
    12061 gtgagaaata gtgcgtggtg tcgatttctt gcaatttggc aagaacatcc gcatactcgt
    12121 agcgagtacc gaccaataag tcttcgatgt ccgatacatc accaattccg aagaaatctc
    12181 cgtaaatctt gatagattcg atgactgatt tttcagcctt gatgtaggtt gtgattttac
    12241 cagctgggta acgcacgcta cgctccacag tgtattctgg tgtttgaccg taggtccaat
    12301 cccatgtcgc aaactgggta tcacggattt cttggatacg aaccaagtca ccttctgaca
    12361 agacatactc gtccatgtct gggtattctt gcttcatctg gttgaggata gcatccttga
    12421 actccagaac ggtcatcttc tcaggcaatt cgttgttgat attggtcaca cgggcacgga
    12481 ctgacttgac acccttggat tcaatcttgt ccttgctgac cttgagggca cttgccaata
    12541 cagacatatc cacatcaaag agcaagcaac cgtggtgcat catgcgacct ttggcatagg
    12601 cttgagcgtt accacagatt ttctttccgt cgatttcaag gtcattacga ccggtgaaat
    12661 tggcctctac acccagtgta gcaagggtat caatgacagg cttggagaag gttttgaagt
    12721 caaaggctcc ctcgtctgcc ttatttgaga taatggtgta gttgaggtta ttgaggtcat
    12781 gatagacagc gccaccacct gacagacggc gaaccacatg gatgcccttt tcgtctgtga
    12841 attccttgtt gatttcttca atagcattct gatgcttacc gataataatg gcaggctcat
    12901 tgatccagag aataaaaatt tcgtcaatat ctgttaattc cttgaaggca taggcttcga
    12961 gggcgatgtt ataagctggg tcattgctgt tgtttacgat gtatttcatg caaaactcct
    13021 ttattgttaa ctaattttta ttatagtgga aacgataaca aaagacaagt ccgaagacct
    13081 gtctctcgtg ttttatccca catccgctcc gtttgtagcg atgacttttt tataccattc
    13141 aaatgatttt ttcttagagc gtttgagtgt gccatttccc tcattgtcac ggtcaacgta
    13201 gatgaagccg tagcgttttt tcatttcgcc cgttccggca gacactaggt caatacagcc
    13261 ccaagtggta tagcccatga gttcaacacc gtcttcgaca atagcatctc tcattgccaa
    13321 gacatgttgg cggagatagt caatacggta gtcgtcttcc acatagccgt tttcatctgg
    13381 cgtgtcgact gctcccagac cattttcaac cacaaagagt ggtttttgat agcgatccca
    13441 tatggaattg atggtaatgc ggaaaccgag cgggtcaatc tgccagcccc attctgatgc
    13501 gtctaggtaa ggatttttaa tggaagcaaa gatattgcct gctgtttttt ccttctctac
    13561 aggatctcca gaagccacac ggctagagta gtaagagaag gaaataaagt ccaccgtatg
    13621 ctctttcagg agtgctaggt cttggttggt catttcaatc tcgatacctg cacgctccca
    13681 cttcttcttg gcatagtttg gataatagcc acgtgcctga acatcgataa agaaatagtt
    13741 ctcacggtct tcttgcagag cttcccatac atcacgtggg tggcaggtgt gtgggtagta
    13801 ttgacctgca gccaacatac aaccgacctt gttttccgga tcgatttcat gggctaattt
    13861 agtagcaatg gcagaggcaa caagctcgtg gtgggcagcc tggtatttaa cctgttccac
    13921 attttcgcct tcttcaaaat acagacctgc ccccataaat ggtgcgtgga gaatcatgtt
    13981 gatttcgttg aaggtcagcc agtatttgac caagcccttg tagcgtgtga agagggtacg
    14041 gcaaaggcgt tcgtaaaatt caagcatttt acgatttcgc cagccaccgt agttctcaat
    14101 caagtgcatc gggcagtcaa agtgggtgat ggtcaccaat ggctcaatac catacttgtg
    14161 acattccttg aacaagtcct cgtaaaattg caggcctttt tcattaggct ccaattcgtc
    14221 tcccttaggg aaaatacggg accaggcgat tgaaagacga taggtcttaa agcccatttc
    14281 accaaagaga gcaatatcct ccttgaagtg atgatacata tcaatagact ccttggctgg
    14341 atagaaataa ccgtcctcaa agtcaaacat cttcttacga ccagtgataa tggcagctcg
    14401 gtcttggcca attggaacta cgtcaacatt ggcaagtcca cgtccgtcta catcgtaagc
    14461 tccttcacat tggttggctg ctgttgcacc accccataaa aatccttttg gaaatacttt
    14521 tgttactgtc attttttctc cttttccttt ccttcttttt gatagagatt ttctttatta
    14581 acgggatcca caatgctttg caaatcgtag tgaccgtctt cataagtaaa agtcagtatg
    14641 gcacaattac gcatccgaac ttgcggataa tctgtcaata cctgcctgaa aaattgggct
    14701 atggcagctc catggctaac tactaaaaca gattgattgt ctgcttcttc catgatgccc
    14761 ccaatggttt cgaccattcg ttgtcggaca tcttgatagg attcgcctcc aaaggtgaca
    14821 aaataatcac cgtagccaat gtctccctgt aagggaggat tgaggtattc ctgttgaccc
    14881 tcgaaggctc cgaaatcctg ttctttcaac cctttcaagc ggatataatc cgtcctccct
    14941 gtgaccaatt ctgccgtatc acaggcacgt tcttgggttg aagaatagat tttatcgaac
    15001 tcaatccctt gctcttgaaa atactggtga gcagctagtg cttgctctct tcccagttct
    15061 gtcaggggag aatcacaggc cccttgaatc cgaccttgtt gattaaaccg agtttgtcca
    15121 tggcgcatca agtaaagata gttcatacta aacctcctta cttaaaatca taaaccttaa
    15181 aatcatgtgc ggaatcaatc agtttttcaa atcgtatttg ctcattttca acagtcagct
    15241 ggcaaatggc acagttaggc aggaagacgc ctggtgggaa ggtgacctcc aaaacctgca
    15301 ccaatcccca aatggctgca ccatgactga ccatgaggat tggatcagct ggatcgcttt
    15361 ccgcgacaga gagaatcgct tctttgacac gacttccaac tgctcgaata tcctcgcctc
    15421 caaatggaac caagaggtct tcgaaagagc gagcacctgg acggtgcttg ggaagcaatt
    15481 cttcctctct ggcttcaaaa atcccgaagt tcatctcctt gagccccttg agctgttggt
    15541 agggagcatc cgtaactaat tttagtgtat cagtagccct ctcttgcgtg gaagaatagg
    15601 cactggtaaa gacgattccc tgctccttaa aatactgacc aacggcttgg gcctgagcga
    15661 taccacgttc tgtcaaggga gaatccgacc aaccctgcac tcgtttttgc gtattaaaga
    15721 gggtttcacc atgacgcatg agataaatcg tttttgtcat aagacctcct atttttctgt
    15781 caatacattg gctccattgc tggcaatgac atctttatac cagtagaatg atgccttccg
    15841 tgatcggtca aaactgccct ttccttgatc gtccgcatcc acatagataa agccgtagcg
    15901 tttggacatc tcactggttg aagcagaaac cagatcgata cagccccaag gggtatagcc
    15961 cataagttcc acaccgtctt caatagcttc atacatttgc tggatgtgtt tttctaggta
    16021 ggcaatacgg tagccgtcct caatactgcc gtctgcctcc accttatcca aggctcccag
    16081 accattttcc acgatgaaga ggggaacttg gtaacggtca taaagcttgt tgagggtgat
    16141 acgaagtccg acagggtcta tctgccagcc ccagtccgac gcttccagat aaggattggc
    16201 ctctcctagt atcaaattac ctgccgttgc cttgtccttg tctggaagag cacgggtgat
    16261 ggatgtcata tagtaagaaa aactcatgaa atcaacagga tattgtttca aaatcgcttc
    16321 gtctcccggc tcgaacacga cctggatatt gttttctttg aagaagcgtt tcatataact
    16381 tgggtaggct ccacgcacct gcacatctgt atagaagaga ttttcctggt cttttttcaa
    16441 ggccgccaag acatcgtctg gattgcaggt ggctgcatag ttctccatcc gtgccagcat
    16501 gcaaccaacc ttgttttctg ggtcaatttc atgggcagct tttgttgcca aactagaagc
    16561 gataaactga tggtgaactg cttggtacat ggcttctagg tcattctttc cgtcttcgaa
    16621 gagaagaccg cctgatagat aaccaaccat gcccaaaata ttgatctcat taaaagtcag
    16681 ccaatactta actttaccct gatagcgttt gaatactgtc tcggcatagc gaacaaagaa
    16741 ctctatgacc ttacggtttt tccagccacc gtattctaga gcaagatgaa gaggaaattc
    16801 atagtgagat agggtcacca gtggctcgat accatattta ttcagctcat cgaagaccgc
    16861 atcataaaag gccagtcctt cttcattcgg ttctaactcg tctccttttg gaaaaatacg
    16921 agaccaggca atcgacaagc ggaaggtctt gaagcccatt tctgcaaaga gggcgatgtc
    16981 ttccttgtag cgatgataga aatcagaacc ccaacgtttt gggtagagtc cttcttgatc
    17041 tgccaaggca aattccacat cagcccgcga gacaggctta ccaaagtgac tcatatcctt
    17101 ccgcttatct ggatcgaccg ctatataggt atcggaagtg gctggaccac gacctcccaa
    17161 attccaacca ccttcatatt ggttggccgc tgttgcaccg ccccaaagaa atccttttgg
    17221 aaattgtttt gtcatagcac aatactcctt ctgactttat tagctatata atagcaaaag
    17281 aacccccttc attcctacgt gataatatgc caaactgata ctattctatg ctatttccaa
    17341 ttatcactat tcttaaaaca ttagataata tcccaattaa aacttggaac aagggagagg
    17401 ttttctctcg atcagtatta gcaaaagtag tagagatatt tttaagcctc catatgtatt
    17461 gatagtcgct tcatcaagtc agctataaaa aatcttcttc tcttgcccta cctcagctcc
    17521 tctgattta
//
LOCUS       DAZQCJ010000034        16651 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000034 DAZQCJ010000000
VERSION     DAZQCJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16651
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.034"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            121..780
                     /gene="liaF"
                     /locus_tag="ACHU66_001049"
     CDS             121..780
                     /gene="liaF"
                     /locus_tag="ACHU66_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFR3389341.1"
                     /translation="MIFTMAAFDVVANEASRTLLLFSALLLVIWYFLGRKVNSVLLVS
                     SLSLLFSVFVLNPFFIIGIMLLVVYMLINFFSRYEKRNQYTHIVFSDYSLQVQKEKNR
                     WFGNHDHSQDRFGFEDINIVRLFGNDVVDLDKTVLVGRDNIVVIRKTFGRTKIIVPID
                     VEVALSATSIYGRVSFLGESYWDLRNESIAVNSSDYQESHKRVKVVTNNILGDVEVVR
                     V"
     gene            777..1793
                     /locus_tag="ACHU66_001050"
     CDS             777..1793
                     /locus_tag="ACHU66_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR3389342.1"
                     /translation="MRKRTYLLLAWFACLIVFVVIASTLPLLNYSLLDVTLWISTEQL
                     VFTLILLVIVLTLFLAVMVQTVSLASTQVMRTKLQAILKNKSIRTDTESDQLLLQLSD
                     KVRNLTRQVQLVDNQDLVKKEEIVEGERKRIARDLHDTVSQELFATSMILSGLSSNLS
                     TLPQETLQEQLMLVKDMIESAQRDLRILLLHLRPSELESKTLVEGFELILREVSDKSN
                     IIVHFQHEVEELPKLIEEHLFRIAQEIISNTLRHAKAKHLDVYLLQKETELQLKMTDD
                     GIGFQQGADDELSYGLQNIRERVEDMAGTIKIRTAPNKGVAIDIRIPLLKGKEDEYDS
                     SDAG"
     gene            1765..2406
                     /locus_tag="ACHU66_001051"
     CDS             1765..2406
                     /locus_tag="ACHU66_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFR3389343.1"
                     /translation="MNTIRVMLVDDHEMVRLGLKSYLNLQPDVEVVAEASDGEEGLAK
                     ALEVKPDVVVMDLVMPKMTGVEATLALLKEWPQAQIVILTSYLDNEKIYPVLEAGARG
                     YMLKTSSADEILAAIRKVALGEYAIETEVEKKVEHHKRHPDLHDDLTAREREILTLLA
                     KGYDNQRIADESFISLKTVKTHVSNILSKLAVSDRTQAVVYAFQHGLVAQEDQ"
     gene            2456..3856
                     /locus_tag="ACHU66_001052"
     CDS             2456..3856
                     /locus_tag="ACHU66_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HFR3389344.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRVKNTGYIPESIQQVFNSLKE
                     KGILTGIASGRTPYGLVPEIKALKPDFFAMINGSYVEDAKGQVVYHQPMPQNIVESVL
                     NWAKEIGIEYGMLGSQKGTLSARTDRISQVIDLIYEGLEINPTFYKENDIYQLLTFEK
                     DGHEVELPEELQAELRSVRWDAISSDIVLKGSSKATGVAKVVEKLGLKPENVLVFGDG
                     LNDIELFDYAGISIAMGHSHPELQKHADYITKKVEEDGIFDALEKLGMVEKEKYFPQL
                     DLENVTGPVAHIKTNHGKLTVKLFPEIAPKTVANFVALSKDGYYDGIIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGTAFEDEFSMEAFNLRGALSMANAGPNTNGSQFFIVQNQNFP
                     YNAKELERGGWPKEVAEAYVKNGGTPHLDQRHTVFGHLVDEESFVVLDAIAAVATDSA
                     DRPHEDVVIETIEIED"
     gene            3858..4229
                     /locus_tag="ACHU66_001053"
     CDS             3858..4229
                     /locus_tag="ACHU66_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HFR3389345.1"
                     /translation="MKIGDKLKGTVTGIQPYGAFVQLENGTTGLIHISEIKTGFVDNI
                     YDHLKLGQEVLVQVVDFDEYTEKASLSIRTLEEEQQKIPRHHRFTNERNKIGFSPLAK
                     QLPKWIEEAKGYLKEHKEKKV"
     gene            complement(4256..5182)
                     /gene="cysK"
                     /locus_tag="ACHU66_001054"
     CDS             complement(4256..5182)
                     /gene="cysK"
                     /locus_tag="ACHU66_001054"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR3389346.1"
                     /translation="MAIYQNITQLVGKTPVIKLNNIVPEGAAEVYVKLEAFNPGSSVK
                     DRIALAMIEDAEKAGTIKPGDTIVEPTSGNTGIGLAWVGAAKGYKVIIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAKEIAEEKNGWVPFQFANPSNPKVHEDTTGQE
                     ILEDFGTTGLDAFVSGVGTGGTVSGVSHVLKTANPDIAIYAVEADESAVLSGEAPGPH
                     KIQGISAGFIPDTLDTSAYDGIIRVKSDDALATGRAIGGKEGFLVGISSGAAIHAAIE
                     VAKELGTGKKVLAILPDNGERYLSTALYEFND"
     gene            complement(5412..6569)
                     /locus_tag="ACHU66_001055"
     CDS             complement(5412..6569)
                     /locus_tag="ACHU66_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR3389347.1"
                     /translation="MKYTKTKYPNIFTYETQKGLRYYVRRGYFVNGDKKEFSKSGLRS
                     LKDAQRILRDIEERIYHDEMDVNLELTLNEYWEIYSAKKEKTGQWNDTSIYTNAGIYR
                     TMIKEKWGNLPLKKINRNDYEEHLAEMLGQYRRNSVLTSNRLLNSILNDAVKNGNLRQ
                     NKLSGIYLGESELEPLNKELKIDDFQTWIKTAEELLSTTHFAFVYLTIFGLRRGEVCG
                     IRFMDVTFDSNNRAVLNIRDSRSNRTKDGAGRTKTESSVRYVVLNDRGSELLLQIMKT
                     AKQVKKKADVIVKQEKDYLSIRIKNNKYYLERPAFLNKIFKRVSEECGIYITPHIMRH
                     FFTTQSLIAGARPEDVMHFLGHASLQTTKQYTHIKEERAHNVTDLFDKKVL"
     gene            complement(6766..7497)
                     /locus_tag="ACHU66_001056"
     CDS             complement(6766..7497)
                     /locus_tag="ACHU66_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389348.1"
                     /translation="MKKNNFVKIILIIFAIALVVAIAAMLMPVAFVGGLGAVWYFTKK
                     KPDSNKRNISIAFAVVGLLGSIFITPAMLNNQKNTSTTVATAPSISSTSSTETSTSTS
                     STTEASSSTEESTEPTTKNDGPEYTEESNAAFATAFMNALNQSLADNGVDINVRVQYY
                     GKNLIHVIVPQDFKYEPNTSIQKVADSVFQAKENYFTEWAIDNGYDLGYTYAPKLYIN
                     AEDDTTLAEEEGILERKMELKIDNN"
     gene            complement(7545..7928)
                     /locus_tag="ACHU66_001057"
     CDS             complement(7545..7928)
                     /locus_tag="ACHU66_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFR3389349.1"
                     /translation="MMTPESVCAERGIDLVYFDGRDSDKKGIYNKRANMIAVDAYLDE
                     IQHKKVIYHELGHENHDPAQYDRRREQYELQADRNMIHYLVKEELSLMDDVKEFNYVH
                     FMEKYDLKTIVNETMVIEEFNNLVN"
     gene            complement(7935..8483)
                     /locus_tag="ACHU66_001058"
     CDS             complement(7935..8483)
                     /locus_tag="ACHU66_001058"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR3389350.1"
                     /translation="MILSHFLDLDYMIHDYKSSIKRFFDFFSSFFGICTKSLDIKSLF
                     AYNIPMNREVKTMADAKIKYPEVGQRIRELREMRGFEQLDIANQLGYKSQSTISKWES
                     GVNLPTGKKLILLAEMLDTSTDYILHGKISDTSTKDTTPTIDFKEMAAESMSYDGMPL
                     NDEDIDLIASILETRMKNRDKE"
     gene            8500..8700
                     /locus_tag="ACHU66_001059"
     CDS             8500..8700
                     /locus_tag="ACHU66_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR3389351.1"
                     /translation="MAKPKITIAELRAKNNKMSQKELASKIGVAYQTIGAWEDDITVI
                     KGDNLLKLCEFFGVSSSDLLGR"
     gene            8770..9096
                     /locus_tag="ACHU66_001060"
     CDS             8770..9096
                     /locus_tag="ACHU66_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389352.1"
                     /translation="MVENLLGIPCGHNPVVLGSVVDGNTKIFGEIVTVFSGDIIFGNL
                     KKLDVYTEGEILKLSDLYDKYKDYISPNDSLYVVVTNGLSGTIYDCGHTRRGIWHIFA
                     KTAGYA"
     gene            9170..9424
                     /locus_tag="ACHU66_001061"
     CDS             9170..9424
                     /locus_tag="ACHU66_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389353.1"
                     /translation="MDKIFVELSDWIKSIIRDVVNEILLEKDNDDGFPEMMNRKDCIR
                     FLKVDGTVFDKYRKLPNFPKEQEGTKWKKRAIKIWLSEKD"
     gene            9465..9713
                     /locus_tag="ACHU66_001062"
     CDS             9465..9713
                     /locus_tag="ACHU66_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389354.1"
                     /translation="MTEAIMTLSLFAVPLLAVGIVEHRKAEKRRMHKEFEEIRRRDYL
                     YGFKAGMGYQSTCDTEKARNGLKRDAQQVDKEMARYAG"
     gene            9703..9855
                     /locus_tag="ACHU66_001063"
     CDS             9703..9855
                     /locus_tag="ACHU66_001063"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389355.1"
                     /translation="MQDNHTTIEISVEEYIQLRNKVNDLQTENRFLRTIVDSVAVVMK
                     NSGMVR"
     gene            9860..10201
                     /locus_tag="ACHU66_001064"
     CDS             9860..10201
                     /locus_tag="ACHU66_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389356.1"
                     /translation="MTQAERIREYYKQHPAASYDEVAEALKTSNSNVRANVSKDIKAG
                     RCVRLEDKSLDYSMHYIKNEALADLINWKNDTRREWVDMLTRAAEKETDNNTMRLLIK
                     EANKLMKEVTE"
     gene            10201..10680
                     /locus_tag="ACHU66_001065"
     CDS             10201..10680
                     /locus_tag="ACHU66_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFR3389357.1"
                     /translation="MASIYELTGIFKQIAEMEGIDEETKLDTLESIDWTEQFEEKVEN
                     TVKVIKNKEADVDQLKEEIDRLTKRKKSIENDITRLKTGLQGAFEITGHDKIKTLLFT
                     VSLANNQPSVVVDEDLLPKKYFIQTLKPDKTAIKELLKAGKKVKGAVLQESRSLRIR"
     gene            10677..10922
                     /locus_tag="ACHU66_001066"
     CDS             10677..10922
                     /locus_tag="ACHU66_001066"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389358.1"
                     /translation="MMGTLFEQRPRFEQLSTPGYRIALMANELTDLFGISFKEALKCI
                     ELDHKINDYDVKDGQLAGFGELLRDYLEVKQNENPSN"
     gene            10903..11367
                     /locus_tag="ACHU66_001067"
     CDS             10903..11367
                     /locus_tag="ACHU66_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389359.1"
                     /translation="MRILAIDPSSNRIETSTTGIVLLDNAGLVSYWVVPFGARNFSRW
                     FREVGRDLEYDVVIVEEYQVRDNDYSRDNSVAETVEAVQACFPNVELVRNAGYVSDIP
                     DQLLRKLGLWTFDKSHHQDVRAAARLALFWAQRKDIEEVIQDIGNRITQMAS"
     gene            11336..12508
                     /locus_tag="ACHU66_001068"
     CDS             11336..12508
                     /locus_tag="ACHU66_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFR3389360.1"
                     /translation="MEIELRKWQAEAVKRSDRDCPGIFLEGLGGRGKTICAFEIAKHK
                     GAKKVLVLNNRLSILNGWIDTHNKLYSQDFRLEAMTDKRMQNIVANGEVLEFDVLIVD
                     EWQNMSSEANYKAYKKIRRDYSIGLSATPIRKKGQNFYPLEKTIFGQAEPNNKFDWQK
                     QHGKMKYDRFSYSKEKWEDFRDYESYVSGLPNFFRWEEIEEIEGAEENNGFEVIFEPM
                     WCLPANPEELDQLRRVNIVGKNGKYAMAKQSFGRKTFERYLTQTGFEVDFPKLKAVNA
                     DTPMLLQLDILLAKQSEMLIVSKSKQIVEVIYERHPEIGIWTGDKKEGHDRTNMVATS
                     QVLGVGVDGLQHRFKTIVVLDPSQPSDGDYDDYRQLLWRITGSRQQHDVRVVEFYF"
     gene            12582..12848
                     /locus_tag="ACHU66_001069"
     CDS             12582..12848
                     /locus_tag="ACHU66_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389361.1"
                     /translation="MSEIKNNPHSLATKAGLVEEIRGALSLPYEYYLKQESEIKALAK
                     IHDCEIILVDAEYTLTYPNGEEVSKIIPKESNLFDDLMEAFKQL"
     gene            12858..13556
                     /locus_tag="ACHU66_001070"
     CDS             12858..13556
                     /locus_tag="ACHU66_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFR3389362.1"
                     /translation="MTVKESPIFATLCSIQTELVAPKGQYNSFGKYNYRSAEDILEAL
                     KPLLKKHDASLVLNDDIEQIGDRYYVRATVTLFACGSSISAQASAREEDTKKGMDGSQ
                     ITGTASSYARKYALNGLFAIDDNKDPDTDEYANQTGKQAQAQKAAQSKSTSNPKQNQN
                     ASGSVKYITGAQAKKLREDIKNIAEASGGPVNTVGVWFIGQLGVDKIESIPADRLKEA
                     QDLIAKTKKAKGME"
     gene            13556..13852
                     /locus_tag="ACHU66_001071"
     CDS             13556..13852
                     /locus_tag="ACHU66_001071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389363.1"
                     /translation="MGFTELGRKRPIKIQGDTYDEILIYKYKGEVWGVCYKGREIDCV
                     YNYTKEDFFYSRIWGMTLKEIVKKIIQPLKRTEPGIFAMIDYELVRKALKVEKL"
     gene            13849..14247
                     /gene="ssb"
                     /locus_tag="ACHU66_001072"
     CDS             13849..14247
                     /gene="ssb"
                     /locus_tag="ACHU66_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019778239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR3389364.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFTLAVNRNFKNQDGER
                     EADFINVVIWRQQAENLANWAKKGALIGVTGRIQTRSYDNQQGQRVYVTEVVAESFQL
                     LESRGQQSNSQDGSFGNSSPMDIQDEDLPF"
     gene            14258..15085
                     /locus_tag="ACHU66_001073"
     CDS             14258..15085
                     /locus_tag="ACHU66_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFR3389365.1"
                     /translation="MGMKKAALAYQKKGFSVIPISPSNKQPMIKFADKPAMTAQEIED
                     FWSQYPDSNIAVRTDKFFVIDIDLHGKHNGYESLANWEHLNLITPTLQAKTASGGKHI
                     FYFKHPDVSMTQMIGFLPGVDVKAHPNNYVLVAPSKTPKGEYAWDLEKSKEGGTMVTA
                     SRALVMAIKQEYLKKNNRSELDDIYYQIRNGAGKRNRTTEVFEMIVKGFGEEGSRNDT
                     AAKFAGTLLARSVDPNCVLELARIANNNSADPLSDRELSRTVDSMVQKHMRGGGSDW"
     gene            15069..16448
                     /locus_tag="ACHU66_001074"
     CDS             15069..16448
                     /locus_tag="ACHU66_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFR3389366.1"
                     /translation="MAVIGDVVNISVKQFTRTKKKVLDENGEKVEIDAIVSDSPRNVL
                     LAMKSDNKLNDFLRHNEFTGEHEIVADVKLDAISMRKGQLPSAFESYLSVYLENHFKV
                     VFKTRALQDGIEAFFAEKTYNPVQEYMEHAYDSWDHKERLNQVFQTWLGAEDSIYVQK
                     IAEMFFVGAVSKVFNPWVKFDYTLDLVGGQGAGKTTFLQKIAVDWYTDSAKDFMDKDN
                     YEIMLKSLIVNDDEMVASRKTTFDELKAFVTKTDLTFRRSYGRRAEKFPKNFVIARTS
                     NKVEYLGDKTGERRFLPILVDAAKQFVKPFDMTDNDVLQLWGEAVAIYKKGFTLTFDE
                     DFEDELAVYKERFTYRDEAENQIYDYLDMLVPEEWESMSVVQQHQYTWAYFNNGVYRN
                     DAGFIYEGVKPQESVSSKQILKNVFDIDVAKGDKLARKIKLIMDNNQDWEYKVKKVNG
                     KAIRGYFRK"
BASE COUNT         5177 a         3033 c         3709 g         4732 t
ORIGIN      
        1 atatcagtca gtcctatatt tcctactttg gatggagttc ttttacttat tttttgttac
       61 aatagtatag aaaggatgga gaagatgcgg aaagttcaat tttttctact aatagaaagt
      121 atgattttta cgatggcagc ctttgacgtg gtagccaatg aggccagtcg gaccctgcta
      181 cttttctctg ccttattact cgtcatttgg tacttcctag gaagaaaagt caacagtgtt
      241 ttactggtca gctccttatc tctattattt tcggtctttg ttttaaatcc cttcttcatt
      301 attgggatta tgctactagt cgtgtacatg ctgattaatt tcttttcacg ttatgaaaag
      361 agaaaccaat atacccacat tgtttttagt gattattctc tacaggttca aaaagagaaa
      421 aatagatggt ttggcaacca tgaccactca caagataggt ttggttttga agatattaac
      481 attgtacgct tgtttggcaa tgatgtagtt gatttggata agactgtatt agttggcaga
      541 gataatattg ttgtaattcg taaaacgttt ggacggacaa aaattattgt tccaattgat
      601 gttgaggtcg cactttcagc aactagtatc tatggacgag taagtttcct gggtgaatca
      661 tactgggatt tgcggaatga aagtatagcg gtcaatagtt ccgattatca agaatcacac
      721 aaacgtgtaa aagtcgttac taataatata ctgggagatg tggaggtggt tcgcgtatga
      781 gaaaacgtac ctacctactc ctcgcctggt ttgcctgtct tattgttttt gtggtgattg
      841 cttccaccct cccactttta aattattcat tgttagatgt tacgctctgg atttcaacgg
      901 agcagctcgt gtttactctc attctactag ttattgtttt gacccttttt ctagctgtaa
      961 tggtacaaac tgtcagccta gcctccactc aggttatgcg gacgaagtta caagctatct
     1021 tgaagaataa aagtattcga acagataccg aaagtgatca gctcttgctc cagttgtctg
     1081 ataaggtcag aaacctcacg cgtcaggtgc agctggttga caatcaagat ttagtcaaga
     1141 aagaagagat tgttgaggga gaacggaaac ggattgccag ggatttgcat gatacggtta
     1201 gtcaagagtt atttgcaacg agcatgattt tgtcaggatt atcgtccaat ctatccactc
     1261 ttccgcagga aactttgcag gaacagctga tgctcgtgaa agacatgatt gagtctgccc
     1321 aaagagacct acgcatccta ttattacatc tacgcccaag tgaacttgaa agtaaaacct
     1381 tggttgaagg atttgagctg attttgcgtg aagttagcga caaaagtaat attatcgttc
     1441 atttccaaca tgaagtagag gagttaccga aactaattga agaacatctc ttccgcattg
     1501 cgcaagaaat tatcagtaat accttgcgac atgccaaagc caagcatttg gatgtttacc
     1561 tcttgcagaa ggaaacagaa cttcagttga aaatgacaga cgatggtatc ggcttccaac
     1621 aaggggcaga tgatgaatta agttacggtc ttcaaaacat acgtgagcgg gttgaagaca
     1681 tggctggaac gataaaaatt cgcactgctc caaataaggg agtggcgatt gatattcgta
     1741 tcccactatt gaaaggaaaa gaagatgaat acgattcgag tgatgctggt tgacgaccat
     1801 gagatggttc gtctaggatt gaaaagttac ttaaatttac agccagatgt tgaagtagtt
     1861 gcagaagcta gtgatggcga ggaaggtttg gctaaagcat tagaggttaa acccgatgtt
     1921 gtagtgatgg acttggttat gcctaagatg actggcgtag aagcaaccct ggccttgctg
     1981 aaggaatggc cgcaagcgca gattgtcatc ctgacctcct acctagataa tgagaaaatc
     2041 tatcctgtgc tggaagcagg agctcgtggc tatatgttga aaacgtcaag tgctgacgaa
     2101 atcttagccg ctatccgcaa ggtagctctt ggagaatatg ctattgagac agaagtagag
     2161 aaaaaagtag agcaccataa acgccaccca gatttacatg atgacttgac agctcgtgaa
     2221 cgcgaaatct tgacgctgtt agccaaggga tatgataatc aacgaattgc agatgagtct
     2281 ttcatttccc taaaaactgt taagacccat gtttccaata tcttatccaa actagctgtt
     2341 agcgatcgta ctcaagcagt tgtttatgct ttccagcatg gtttggtggc gcaagaggac
     2401 caatagtggt ataatgaaaa ttagttaatt ttcaaccaaa ggatagtgga attatatgga
     2461 tgcaaaactc agatataagg caaaaaaaat aaaaatagtc ttttttgaca ttgacgacac
     2521 gctacgtgta aagaatacag gctacattcc tgaatccatt cagcaggttt tcaactcact
     2581 gaaagaaaag ggaattttga caggaattgc gtcaggacga acgccttatg gtttggtgcc
     2641 agaaatcaag gccttaaagc cagacttttt cgctatgatt aacggctcct acgtagaaga
     2701 tgccaaaggt caggtagtgt accatcagcc aatgccccaa aatatagtag aatctgtatt
     2761 aaattgggct aaagaaattg gaatcgaata tggtatgctt ggtagtcaaa aagggacact
     2821 ttctgcacga actgaccgca ttagtcaagt aattgacttg atttatgaag gtttggaaat
     2881 aaacccaact ttttataaag aaaatgacat ctatcaattg ttaactttcg agaaagatgg
     2941 tcacgaagta gaacttccag aagagttgca agcggaattg cgtagtgttc gttgggatgc
     3001 tatttcatcg gacattgtgt taaaaggctc ttccaaagca actggtgttg ctaaggttgt
     3061 tgaaaaacta ggcctgaaac ctgaaaatgt cctagtgttc ggagatggac tcaacgatat
     3121 tgagttattc gattatgcag gaattagtat cgctatgggg cattcgcatc cagaactgca
     3181 aaagcatgca gattatatca caaaaaaagt agaagaagat ggcatttttg atgccttgga
     3241 gaaattaggt atggtagaaa aagaaaaata tttcccgcaa ttagatttgg aaaatgttac
     3301 aggaccagtt gcgcatatca agactaacca tggtaagtta acagtaaaac tctttcctga
     3361 aattgcaccc aaaactgtag caaatttcgt agctctttct aaagatggct attatgacgg
     3421 gattatcttt catcgtatta tcaaagactt tatgattcaa ggtggcgatc caaccggaac
     3481 aggtatgggt ggtgaatcta tttatggtac tgcgtttgaa gatgaatttt caatggaagc
     3541 ttttaatctc agaggagcct tgtcaatggc caatgcagga ccaaatacaa atggaagcca
     3601 attctttatc gttcaaaacc aaaatttccc atacaatgcg aaagaattag aacgaggcgg
     3661 ttggcctaaa gaagttgcag aagcctatgt caaaaatggc gggacaccac atctggatca
     3721 acgccacaca gtgtttggtc atctagttga tgaagaatcc ttcgtcgtct tggatgctat
     3781 cgcagcggtt gcaacagatt cagcagatcg tccacatgaa gatgttgtaa ttgagaccat
     3841 cgaaatagag gattaagatg aaaatcggag ataaactcaa ggggacggta acgggtattc
     3901 aaccgtatgg agcctttgta caattagaga atgggacgac gggcctaatc catatctcgg
     3961 aaatcaaaac aggttttgtt gataatattt atgatcattt aaaattgggg caagaggttt
     4021 tggtacaggt agttgacttt gatgaatata ctgaaaaggc tagtttatca atccggacct
     4081 tggaagaaga acaacaaaaa attccgcgtc atcatcgttt tacaaatgaa cggaataaaa
     4141 ttggtttttc ccctttagca aaacaacttc cgaaatggat tgaggaagct aaaggctatc
     4201 tcaaagaaca caaagaaaag aaggtatagg atgcattcct aaccttcttt cttttttaat
     4261 cattgaactc ataaagagct gttgacaagt aacgttctcc gttatcaggt aagattgcca
     4321 acactttttt tccagttccc aactccttag cgacttcgat agcagcgtga attgctgcac
     4381 ctgaagaaat accaactaag aatccttctt ttccaccgat agcacgacct gtagctaagg
     4441 catcatcgga tttgacacga ataatgccat cataggcgct agtgtccaaa gtatctggaa
     4501 taaaacctgc cgaaatacct tggatcttgt gcggaccagg tgcttcacca gataagacag
     4561 ctgattcatc agcttcgaca gcatagatag caatatctgg atttgctgtc ttcaaaacat
     4621 gcgaaacacc tgaaacggta ccaccagtac caacgccaga tacaaaggca tctaaaccag
     4681 ttgttccaaa gtcttccaaa atttcctgtc ctgttgtatc ttcgtgaact tttggattgg
     4741 atggattagc aaactggaaa ggaacccagc catttttttc ttcagcaatc tcttttgctt
     4801 tggcgatagc ccccttcatc ccttcgctac caggagtcaa gacaagctca gctccataag
     4861 cttggataat cttacgacgc tcaacactca tagtttcagg catgacgata atcaccttat
     4921 aaccttttgc tgctccaacc caagcaagac cgataccagt gttaccactt gttggctcaa
     4981 caatagtatc tccaggtttg attgtaccag ccttctcagc atcttcaatc atagccaagg
     5041 cgatccggtc ttttacagaa gatcctgggt taaaggcctc taatttcaca tagacttccg
     5101 ctgccccctc tggaacgata ttattgagtt taataacagg tgtttttcca actaattgag
     5161 taatgttttg ataaatagcc atatggcacc tccaaataat gttattatac attatatagt
     5221 agaaagtcaa aaaaggataa tataaaaaaa ctatctcgcc gttaagaaaa agctataccc
     5281 agccaatcat ggtatttatg taacaacttg aaaacgttga tactactagc tttttgaaaa
     5341 aatgtttatt gttgatattg tcataacttg gtgtagttgt cggggaattg tcgggaagaa
     5401 gtcggggaaa attatagaac tttcttgtca aatagatcgg taacattatg tgctcgttct
     5461 tctttgatgt gagtatattg tttggttgtt tgaaggcttg catgacccag gaagtgcatg
     5521 acatcttctg gtctggctcc agcaataagg gattgagtgg taaagaagtg acgcataatg
     5581 tgtggagtga tatagatacc acattcctca cttactcgtt taaaaatttt gtttaaaaaa
     5641 gcaggtcttt ccaaatagta tttattattc tttattcgga tgctcaggta atccttttcc
     5701 tgcttaacaa tgacgtctgc tttctttttg acttgttttg ctgttttcat gatctgtaac
     5761 aacagctcac tgcctctgtc atttaaaaca acgtaacgca cagaactttc tgtttttgtt
     5821 cttccagcac catcttttgt cctgttggaa cgagagtctc ttatatttaa gactgctcga
     5881 ttattcgaat cgaaagtaac gtccatgaac ctgataccac agacttcccc gcgtctaagt
     5941 ccaaaaatag tcaggtagac aaatgcaaaa tgtgtggtcg atagtaattc ttctgcggtt
     6001 tttatccatg tttgaaaatc atctattttt aactctttat taagaggttc taactctgat
     6061 tccccaagat aaataccaga caatttattt tgtctaagat ttccattttt gacagcatcg
     6121 tttaaaatgg aattcaaaag tcgattactg gtaagcacac tatttctgcg gtactggcca
     6181 agcatttctg ccagatgttc ttcataatcg ttgcgattta ttttttttag cgggaggttt
     6241 ccccactttt cttttatcat ggtacgataa atgcctgcgt tagtgtagat ggatgtatca
     6301 ttccattgac ctgttttttc tttctttgct gagtagattt cccagtattc atttaaagtc
     6361 agttctaggt tgacatccat ttcatcatga taaattcttt cttcaatatc ccttagaatt
     6421 ctctgggcgt ccttcaagct tcgtagtccg ctcttgctaa attctttctt gtcgccatta
     6481 acaaagtatc ctctgcggac atagtaacgc aatccttttt gggtttcgta tgtaaaaata
     6541 tttgggtatt ttgtttttgt atatttcatt ctcttttcct ttctgtaaga ccaatctgga
     6601 caaggttttt ttgaaaggat attggcatca cctccttaaa gtgatataat tagagtacgc
     6661 aaaaagtcct gctgaaaagc tggtctttgc ttattggact accctacact tcggctttgg
     6721 tcggttggaa gtgtggggat ttttttattt atcgacgttt aaatattagt tgttgtcgat
     6781 tttcaattcc atttttcttt ctaggatacc ttcttcttca gccagcgtag tatcgtcttc
     6841 ggcgtttatg tatagtttag gagcgtatgt gtatccaagg tcatagccgt tgtcaatagc
     6901 ccattctgta aagtaatttt ctttggcttg gaagacgcta tctgctactt tttggatact
     6961 agtatttggt tcatatttaa aatcctgagg cacaattaca tgaatgagat tttttccgta
     7021 atactgcact ctaacattaa tatctactcc attgtctgca agggattgat tgagagcgtt
     7081 cataaatgct gttgcaaaag ctgcgttaga ttcttcggta tattctggac cgtcattttt
     7141 tgttgtcggc tctgtcgatt cctcagttga cgaagaagct tctgtggtac tacttgttga
     7201 agtagaagtc tctgtgctac ttgtagagct gattgaaggc gcagttgcta ctgttgttga
     7261 ggtgttcttt tggttgttta gcattgcagg tgtaataaat atgcttccta acaatccaac
     7321 tacagcaaaa gcgatagaga tgttgcgttt attactatca ggtttctttt tagtaaaata
     7381 ccaaactgct ccaagaccac ctacaaatgc tacaggcatt aacattgcag caattgctac
     7441 taccagcgct atcgcaaaga taattaaaat tatttttacg aagttattct tcttcataaa
     7501 acaacctcct gcccgttttt cgtgcggtgg gcgtgcacgt atttttaatt aactaagtta
     7561 ttaaactcct caatcaccat agtctcatta actatggttt ttaagtcgta tttttccata
     7621 aagtgaacat aattaaattc tttgacatcg tccatcaaag acaattcttc tttgaccaga
     7681 taatggatca tattcctatc cgcttgtaat tcatactgtt cacgtcttcg gtcgtattgt
     7741 gccgggtcgt gattttcatg gcccaattcg tggtagatga ctttcttgtg ttggatttcg
     7801 tccaaatagg cgtcaaccgc aatcatgttc gcacgcttgt tgtatatgcc tttcttgtcc
     7861 gaatctctac cgtcaaaata gaccaaatca attccacgct ctgcgcagac tgattctggt
     7921 gtcatcatag gcaattattc cttatctctg tttttcatgc gagtttcgag tattgaggca
     7981 atgaggtcga tatcttcatc attgagaggc atgccgtcat aagacatgga ctctgccgcc
     8041 atttccttga aatcgattgt gggagtggtg tcttttgtgg aagtgtcact aatttttcca
     8101 tgaagaatgt agtctgtgga agtatctagc atttcagcca ataaaattag ttttttgcca
     8161 gttggcaaat tcactccgct ctcccatttt gaaattgtgc tttgagattt gtaaccaagt
     8221 tgattagcta tgtctaattg ctcgaaccct ctcatttctc tcagctctct aattctttgc
     8281 cctacttcag ggtattttat ctttgcatca gccatggttt ttacctctct attcataggt
     8341 atattataag caaaaagtga ttttatatca agtgattttg tgcaaatacc aaaaaaagat
     8401 gaaaaaaaat caaaaaaacg cttgatagat gatttataat catgtatcat atagtcaagg
     8461 tcaaggaaat gacttaaaat catgtgaaag gagaactgaa tggcaaaacc aaaaatcaca
     8521 attgcagaac ttcgtgcaaa gaataataag atgagccaga aagaacttgc ttcaaaaatc
     8581 ggtgttgctt atcaaacaat tggagcatgg gaagatgaca ttacagtcat taaaggcgat
     8641 aatctattaa aattgtgcga attctttgga gtaagttcgt ctgatttact tggacgttaa
     8701 ttttttaaaa aatacatgat tttttatcat gtagaaacta cccaactaac aaactagaaa
     8761 ggagaaggga tggttgaaaa cttgctaggg attccatgcg gacacaatcc agttgtttta
     8821 ggtagtgtgg tcgacggaaa taccaagatt ttcggtgaaa tcgtgactgt gtttagtggc
     8881 gatattatct tcggaaattt gaaaaaattg gatgtataca cggaaggtga aattttaaag
     8941 ttatctgatt tgtacgataa gtataaagac tatatctcgc cgaacgatag tttatatgtc
     9001 gttgtcacta atggcttatc tggaactatt tacgattgcg gacataccag acgtgggata
     9061 tggcatatat ttgcaaaaac cgcaggttat gcctgacacg caaaaaaacc actgcgggaa
     9121 cagtggctta caaaattaac ttactcaaat tataacacag gaggctacta tggacaagat
     9181 atttgttgag ttgtccgact ggataaaatc gataataaga gacgttgtaa acgagatttt
     9241 actggaaaaa gacaacgacg atggtttccc ggaaatgatg aaccgaaagg attgcatcag
     9301 atttctaaag gtagacggga ccgtctttga taaatataga aaattaccga atttccccaa
     9361 agaacaagag ggaacaaaat ggaagaaaag agctattaaa atttggctta gcgaaaaaga
     9421 ctaaaagacc aatctggaca aggtctgaaa cgaggaaaaa atttatgaca gaagcaatta
     9481 tgacattaag tttattcgca gtgcctttgc tggcagtagg cattgtggaa catcggaagg
     9541 cagagaaaag acggatgcac aaagagtttg aagaaattcg tcgcagagac tacctgtacg
     9601 gctttaaggc gggcatgggg tatcagagta cctgtgacac cgaaaaagct cgtaacgggc
     9661 taaagagaga cgctcagcaa gtagataagg agatggcacg gtatgcagga taatcataca
     9721 acaattgaaa tcagcgttga agaatatatt caacttcgga acaaggtaaa cgacttgcag
     9781 acagaaaatc gttttttgag aacgattgtt gattctgtgg cggttgtgat gaaaaatagt
     9841 ggaatggtga ggtaggaaga tgacacaagc ggaacgcatt agggaatatt ataaacaaca
     9901 tccagctgct agctatgatg aagtggctga agcactcaaa acatcaaata gtaatgtacg
     9961 ggcgaacgtg tccaaagata tcaaggctgg acggtgtgtc cgcttggaag ataagtcatt
    10021 ggactactcg atgcactaca tcaagaatga agcattggca gacctaatca actggaagaa
    10081 tgacaccagg agggagtggg ttgatatgct gacaagagca gcagaaaaag aaactgataa
    10141 caataccatg cgattgctta tcaaggaagc taataaacta atgaaagagg taacggaata
    10201 atggctagta tttatgaact aactggaatc tttaagcaga ttgcagaaat ggaaggtatt
    10261 gatgaagaaa ccaagctgga tacgcttgaa tctattgact ggacggaaca atttgaagaa
    10321 aaggttgaaa acaccgtcaa ggtcatcaag aataaagaag cagatgtgga tcaactcaaa
    10381 gaagaaatcg accgcttaac caaacgaaaa aaatctattg aaaatgacat cacacgactc
    10441 aaaacagggc tgcaaggtgc atttgaaatc acagggcatg acaaaatcaa aacattgctg
    10501 tttactgtca gcttggccaa taatcaacca tctgtggttg tagatgaaga cctgctgcct
    10561 aagaaatatt ttattcaaac tttgaaacca gacaaaacag ctatcaaaga gttgctgaaa
    10621 gctggtaaga aggtcaaggg tgcagtgttg caagagagta gaagtttgag gattagatga
    10681 tgggaacatt atttgaacaa aggccaagat ttgaacaatt gtcaacacca gggtatagga
    10741 ttgctttaat ggctaatgaa ttaactgacc tgttcgggat tagctttaag gaagccttaa
    10801 aatgtattga actcgatcac aaaataaatg actatgacgt aaaagatgga caattagctg
    10861 gatttgggga gttgttgaga gactacctag aggtcaaaca aaatgagaat cctagcaatt
    10921 gatccgtcaa gcaacagaat tgaaacatcc actacaggaa ttgtgctgtt ggacaatgcc
    10981 ggactggtca gctattgggt ggtcccattt ggtgccagaa atttcagtcg gtggtttcgt
    11041 gaagttggtc gtgacctgga atacgatgtt gtcattgtag aagaatacca ggtacgtgac
    11101 aatgattatt ctcgagataa ctcagttgct gaaacagtgg aagctgttca agcctgcttt
    11161 ccaaacgtgg aactcgttcg caacgctggc tatgtgtcag atattcctga tcagttactt
    11221 aggaagcttg gtctttggac ttttgacaaa tcacatcatc aggatgtgag ggctgctgct
    11281 cgcttggcac tattctgggc gcagagaaaa gacatcgagg aggttattca agacattgga
    11341 aatcgaatta cgcaaatggc aagctgaggc cgtgaaacga agcgaccgcg attgtcctgg
    11401 tatttttctg gaaggtcttg gcggtcgtgg gaaaactatc tgtgcctttg agattgcaaa
    11461 gcacaaggga gccaagaagg tcttggttct gaacaatcgg ctgtcaatcc tgaatggctg
    11521 gattgacact cacaacaagc tctattcaca agattttcga ctagaagcca tgacagacaa
    11581 gcgaatgcag aatatcgtgg caaacggtga ggtcctggag tttgatgtct taatcgttga
    11641 cgagtggcag aatatgtcca gtgaagctaa ctataaagct tacaagaaaa tccgacgtga
    11701 ctattcaatc ggcttgtctg caactccaat ccgaaagaaa ggtcaaaact tctatccgct
    11761 agaaaaaaca atctttggcc aggcggagcc aaacaataaa tttgattggc agaaacagca
    11821 cggcaagatg aagtatgacc gattcagtta ttccaaagaa aagtgggaag acttccgtga
    11881 ctatgaaagt tatgtgtcag ggttgccaaa tttcttccgt tgggaagaga ttgaggaaat
    11941 tgagggagct gaggaaaaca atggatttga agtcatcttt gagccgatgt ggtgcctgcc
    12001 agcaaatcca gaagaacttg atcaacttcg acgggtgaac atcgtcggaa agaatggtaa
    12061 gtatgcaatg gccaagcagt catttggccg caagactttc gagaggtatc tgactcagac
    12121 aggatttgaa gttgattttc cgaagcttaa agcggttaat gcagatacac caatgttgct
    12181 tcaactagac attctcttgg ccaagcaatc ggaaatgtta attgtcagca agtccaagca
    12241 gatcgttgag gttatctatg aacggcaccc tgaaatcggt atttggaccg gtgataagaa
    12301 agaagggcat gacaggacca atatggtcgc cactagccaa gtattaggag ttggggtcga
    12361 tgggctccag caccgtttta aaacaattgt agttctggat ccgtcccagc ctagtgacgg
    12421 agattacgat gactatcggc aacttctttg gcggattact ggtagtcgtc aacaacacga
    12481 cgttagggtc gtagaatttt atttttaaaa aacaaaagga gcaaaaaaat gaaaatttca
    12541 aacaagttaa tcgtattgcg tgagaaaaaa atgggtattt tatgtcagaa atcaaaaata
    12601 atccccattc acttgcaacg aaagcaggtc ttgtagaaga aatccgtggt gccctatcac
    12661 ttccatacga gtattatttg aaacaagaat ccgaaattaa agctcttgcg aaaattcatg
    12721 attgtgaaat catcttggta gatgctgagt atacacttac ttatcctaat ggagaagagg
    12781 tttctaaaat tattcccaaa gaatcaaatt tatttgatga tttgatggaa gcattcaaac
    12841 aactttaagg agaatacatg acagtaaaag aatcgccaat atttgcaacc ctgtgcagta
    12901 ttcagacaga gctggttgca ccgaaaggac agtacaattc tttcggaaaa tacaactatc
    12961 gtagtgcaga ggatattttg gaagcactaa agcctttact caaaaagcat gatgcatccc
    13021 tggtattaaa tgacgatatc gaacagattg gggaccgcta ctatgttcgt gctacagtaa
    13081 cattatttgc ctgcggcagt tcaatcagtg cccaagcgtc agcaagggaa gaagatacaa
    13141 agaaaggaat ggatggcagc cagatcaccg gaactgcatc cagttacgct cgaaaatatg
    13201 ctcttaacgg cttgtttgcc attgatgata acaaggaccc tgatacggac gaatatgcga
    13261 accaaactgg aaagcaagca caagctcaga aggcagcaca aagtaagtct acatcaaatc
    13321 cgaaacagaa ccagaacgca tctggcagtg ttaagtacat cacgggagcc caggcgaaga
    13381 agctacgtga agatatcaag aatattgcgg aggcttcagg cggtccggtt aataccgttg
    13441 gagtgtggtt cattggccag ctgggcgtgg ataaaatcga aagtattcca gcagaccgct
    13501 tgaaagaagc acaggatctg attgcaaaaa ctaagaaagc gaagggaatg gaataatggg
    13561 atttacggaa cttgggcgaa aacgtccaat caaaattcaa ggtgatacct atgacgaaat
    13621 cttgatttac aaatataaag gcgaagtttg gggcgtatgt tataaaggca gagagattga
    13681 ttgcgtttat aattacacga aagaagactt cttttactca agaatttggg gtatgacttt
    13741 gaaagaaatt gttaagaaaa tcattcagcc attaaaaaga actgagccag gaatttttgc
    13801 aatgattgat tacgaattgg tgagaaaggc cttgaaggtg gaaaaattat gattaacaat
    13861 gtagtactag tgggacgcat gactcgtgat gcagaacttc gttacactcc gtcaaaccag
    13921 gcggttgcga cttttacctt ggctgtcaat cgaaacttca aaaatcaaga tggggagcgt
    13981 gaagcggact ttatcaacgt agtcatttgg cgtcaacaag ctgagaattt ggcgaattgg
    14041 gctaagaaag gtgctctgat tggtgttaca ggtcgcattc agactcgtag ctatgacaat
    14101 caacaagggc aacgtgtcta cgttactgag gtagttgcag aaagtttcca actcttggaa
    14161 agtcgtggac aacagtcgaa ttctcaagac ggatcatttg gaaattcaag tcctatggat
    14221 atccaagacg aagatttgcc gttctaggag gtgctagatg ggtatgaaga aagcggcttt
    14281 ggcttatcag aaaaagggct tttcggtcat tcctataagc ccttctaata agcaaccgat
    14341 gataaaattt gctgataaac cagcaatgac cgcacaggaa attgaagatt tttggagtca
    14401 gtatccggat agtaatattg ctgtccggac tgacaaattc ttcgtaatcg acatcgacct
    14461 gcacggaaaa cataatggat atgaaagtct ggccaactgg gaacatctca acttgattac
    14521 cccgactttg caagcaaaaa ctgccagcgg tggcaagcat atcttttact ttaagcatcc
    14581 agatgtttcc atgacgcaga tgattggatt cctacctggt gtcgatgtca aagcacatcc
    14641 aaataattat gttctggtcg ctccatcaaa aacacccaaa ggggagtatg cctgggactt
    14701 ggaaaagtcg aaagaaggcg ggaccatggt cactgctagt cgagcactag tcatggccat
    14761 taagcaggag tatctaaaaa agaacaaccg aagcgaactt gatgacattt attatcaaat
    14821 tcggaatggt gcaggcaagc gaaatcggac cacggaggtc tttgaaatga ttgtcaaagg
    14881 ctttggtgag gaaggtagtc gaaacgatac ggcagccaag tttgctggaa cattattggc
    14941 cagaagtgtt gatccaaact gtgttttgga attggcacgg attgcgaata ataactctgc
    15001 tgatccgctg agtgacagag agcttagtcg gacagtagat agtatggtac agaaacacat
    15061 gaggggaggt ggcagtgatt ggtgatgttg taaatatctc tgtcaagcag tttactagga
    15121 cgaagaaaaa ggtcttggat gaaaacggag aaaaggttga gattgatgcc attgtgtcag
    15181 acagtcctcg aaacgtgctg ttggccatga agagtgataa caagctcaat gacttcctcc
    15241 ggcacaatga atttactgga gaacatgaga ttgtggccga tgtcaaattg gatgctatca
    15301 gcatgaggaa aggtcagctg ccctccgcat ttgaatctta tctcagtgtt tacctggaaa
    15361 atcatttcaa ggttgtgttc aagaccaggg ctttgcaaga tggtattgag gcattctttg
    15421 cagaaaaaac ctataatcct gttcaggaat acatggaaca tgcctatgac agttgggacc
    15481 acaaggaacg tctgaatcaa gtcttccaga cctggctagg tgctgaggac agcatctatg
    15541 tccagaaaat agcggagatg tttttcgtcg gggctgtgtc caaggtcttc aatccatggg
    15601 taaagtttga ctacactcta gacctggtcg gtgggcaggg tgctggtaag acaacattcc
    15661 tgcaaaagat cgcagttgac tggtacacgg attctgctaa ggatttcatg gacaaggaca
    15721 actacgagat catgctaaaa tccctgattg tcaatgacga cgagatggtg gctagtagga
    15781 agacgacgtt tgatgagctc aaggcttttg tgaccaagac cgatttgaca ttccgtaggt
    15841 cctatggtcg tcgtgctgaa aagtttccca aaaacttcgt gattgctcgg accagcaaca
    15901 aggtcgagta tcttggtgat aaaactggtg agcgtcgttt cctaccgatc ttggtggatg
    15961 cagctaaaca attcgtcaag ccattcgaca tgacggacaa tgatgtcctg cagttgtggg
    16021 gtgaggcagt tgcaatttac aaaaaaggct tcacgttgac ctttgacgaa gacttcgagg
    16081 atgagctggc agtctataaa gaacgcttca cttatcgcga tgaggcagaa aaccaaatct
    16141 atgattattt ggatatgctg gttccggaag aatgggaaag catgtccgta gttcaacagc
    16201 atcagtacac atgggcttat ttcaacaatg gtgtgtatcg gaatgatgcc ggttttattt
    16261 acgaaggtgt caaaccgcaa gaaagtgtgt catcgaagca gatcctgaag aatgtttttg
    16321 acatcgatgt tgccaaagga gataaattag ctcggaagat taagctgatc atggacaaca
    16381 accaggattg ggagtacaaa gttaagaagg taaatggcaa agcaattcga ggatatttcc
    16441 ggaaataata tgcagaaaaa gtgatgtaac cgaaaacgtg ttttgatgta acttttagtc
    16501 aaaaggttac atcaagttac atcaaggtta caccaatgat gtaaccgttg aaaaactcaa
    16561 tcgtatcaag ggtttaaagg tgttttttta gaaaaaaata aaaaagtggt gtaaccgccc
    16621 taaacccttg gtaatattgg gtttataagt c
//
LOCUS       DAZQCJ010000035        16588 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000035 DAZQCJ010000000
VERSION     DAZQCJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16588
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.035"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..1136)
                     /locus_tag="ACHU66_001075"
     CDS             complement(<1..1136)
                     /locus_tag="ACHU66_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFR3389367.1"
                     /translation="MAVTKIHPIKSTLHFAIDYILDRTKTDSQTLLSTNMCFPTTAHL
                     QFLQTRKEHQVNGSVLARHLIQSFLPGEVDQETAHKIGLELCQKILKDEYEYVLTTHI
                     DKGHIHNHIIFNNVNFKNGKCYQSNKRTYHQIRSTSDRLCKKYKLSVIDEHYQTFRKK
                     YKTRGKSHYEWEQSQNGKGWKPYLQFLIDLNISRSNSWEDFLDRMKKSKCDIKFGKHI
                     SFKGKNASRFTRSKTIGPDYTEERLKERIASKPPHELIDTSTFKDQKGLEFWARKHNL
                     KSMAQGLVELRNLGIYTKDDLEMKIESLADKQISIQDEVKTIDEQMADLEDKMESLHI
                     VQQYQLIYQHYQNNKDNQQYIREYQKEIKAYKMAKNQLLAYDKP"
     gene            complement(1138..1491)
                     /gene="mobC"
                     /locus_tag="ACHU66_001076"
     CDS             complement(1138..1491)
                     /gene="mobC"
                     /locus_tag="ACHU66_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFR3389368.1"
                     /translation="MEKRTRPNQLKIRLSDKELAQVREKFGQSRSKSMRHFVLKSILE
                     TSIYEIDMQPFREIQHLLSKTSTNINQIAKKVNTYSVVYKEDIVTIQNEVQHLSKELH
                     KLQNTLYNRTNQGEI"
     gene            complement(1665..2435)
                     /locus_tag="ACHU66_001077"
     CDS             complement(1665..2435)
                     /locus_tag="ACHU66_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR3389369.1"
                     /translation="MSRDKRLKEITNILHSRKRIDVKQLEKLTFSSTSTLRRDLIYLE
                     EQGLIKRKHGEVVLNAFNTVELAHSIRETENRSEKKIIAELAKDFVGPGMCIYLDSST
                     TVYELCPFLSTIDNLIIFTNGLNTAQLLSELSNPTMKIFVTGGEVKHHSASVVNHSME
                     NSLLEHFSIDLAFCSARGIDEEYVYEASFSQAIAKKSIIDKAKETILLIDSSKFRMSG
                     FFKINNLKQYKTIISDKLPDESLLTSAEKYEVEWIANN"
     gene            2594..4021
                     /locus_tag="ACHU66_001078"
     CDS             2594..4021
                     /locus_tag="ACHU66_001078"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HFR3389370.1"
                     /translation="MKTVLAIDLGATSGRGILYSIENGKLISREVHRFNNSIQKVKGR
                     LCWDIESLVKSVKKCIRISQEQSELSSVGIDTWGVDFALLDVNGEMIAQPVSYRDRRT
                     ESILDEIARYSSLNDLYFKTGNQLMEINTLFQLIACKSQTPEEYFKADKLLMISDYLN
                     FVLTGEVAIERSIASTMQMINPLTQDWNHEILKTFEISELLLPKLVSEGNILGSISDE
                     ILNNEITVINVCQHDTASAVAAIPHEEENLLYVSCGTWSLIGTELKGPILTDSALKYN
                     FTNEAGYDGTTRFLKNCTGLWIIEELKKSFLKLGKDFSYDEITEMVNNVESCVPTFDT
                     DASTFASPGNMIEKIVCYFSDKGIKVSTDPAILFKIVYQSLAEKYKEVIIQLEEITGN
                     IYKRIDLIGGGSKSEYFSQLVADVTGKTVVTGLYEATSIGNALVQFKALGYVEDMKEA
                     KKLVKESITFNHFYPKKEEKNDTLS"
     gene            4005..5792
                     /locus_tag="ACHU66_001079"
     CDS             4005..5792
                     /locus_tag="ACHU66_001079"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR3389371.1"
                     /translation="MTLYPKIGIRPTIDGRQGGVRESLEEKTMQMAMAAKDLIESNLR
                     YADGTPVQCLLASRTIGGQGDAGIVYDEFARDNVVATLSVTPSWCYGTETMDMDPHTI
                     KAIWGFNGTERPGAVYLAAAMSGYAQKGYPAFKIYGHDVQDLDDNSIPADVAEKILLF
                     AKGAIAAGQMKGKSYVNIGASSMGIAGSQVTTSFFEDYLGMLVEFVDMTEILRRMELE
                     IYDKEEYEKALAWIKEYCKEGVDINAGKEFPEVITKSKVIPADKDWEFIAKQTLIIRD
                     ILFGNPRLAEIGWEEEARGRNAISGGFQGQRQWTDWLPNGDFAEAILASTFDWNGSRP
                     VTAFATENDTLNATAMLFGTLLTNKAPIFSDVRTCWSPEAVKRVTGQELTGRAKDGIL
                     HLINSGASALDGSAAARDEAGQPTMKEFWNMTEEDIKACLEATDWCRANYEYFRGGGY
                     SSHFKTQGELPVTLIRVNLVKGVGPSLQIAEGYTCPLEDEVHRILDERTDKTWPTTWF
                     APNLGEPGFETVYDVMNHWGSNHGAFVHGHIGAEVITLASMLRIPVALHNIPRERIFR
                     PSIFDGVGTKDLEAADFKMCEVLGPLYSK"
     gene            5813..6451
                     /locus_tag="ACHU66_001080"
     CDS             5813..6451
                     /locus_tag="ACHU66_001080"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR3389372.1"
                     /translation="MEKERKELIKYGKKLVTEGLTKGTGGNLSVFNREKGLMAITPSG
                     IDFFEIKEEDIVIMDLEGQVVEGEKLPSSEWYMHLIQYQQREDLDAVIHAHTTYGTVL
                     AVLREPLPASHYMIAVAGKDVRVAEYATYGTKELAENAAEAMKDRRAVFLANHGILAG
                     AQDLLNAFNIIEEVEYCSKIYCVAKSMGEPVILPDEEMELMAEKFKTYGQRK"
     gene            6468..7766
                     /locus_tag="ACHU66_001081"
     CDS             6468..7766
                     /locus_tag="ACHU66_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR3389373.1"
                     /translation="MKKGLLKCGALCLFASVILAACGSSSGTSSSSEKAASGDDTVLE
                     FYHGYHHSEEEWPAAKVMRDLYDEFAKKHATGDVEFKPTPVNGSLTDIMNNKVASGEF
                     PDMIDLAGNDVSLAAIEQSLVLDLKPYIDDNGLEKNVGLNYTQNDVNGKIYTVHDQLL
                     TLGLWYNADIFSKAGAKTPDQWTKWSDFTEAMALVRKQGVYAFGAGEPAIRLFNTVLG
                     SSEAGRKLLEGPLTSEGIDSKEFAEALKLVMTEVQANGSANAGGDANVYSTDFTENKS
                     AVFFNGVWAAGGLKDNAALKPGLYAGNVAISSAGGGITISSKLSEEEEKLALEFVKYM
                     TSDEVQTTIFEKVGANPASQAIDTAQIASGSDDATVKLLGEATSMANNADVTVPTVVS
                     VWGGDVRTAIINALTESAADGVNIDQKVKETQDILKALIG"
     gene            7821..8741
                     /locus_tag="ACHU66_001082"
     CDS             7821..8741
                     /locus_tag="ACHU66_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR3389374.1"
                     /translation="MAKKTMTLQNRKAQKQRFIFLFLLPTLVCFFLFYFYSVVTIFAT
                     SFTKWDYTNLTSPEFLGFGNLFANYKYIFSEYPFFLEALKNSITWAIIGVVVQVPLAL
                     SVAIALSKKLAGWKLSRNLYIIPSIISSAAMGLIFLQIYNPNYGVVNQVIRFFNPEFS
                     DAILLTPGLNIIAMTCAYIFFAGTSTIMILGQIFAIPQEIHEAAILDNITGWRREWYI
                     TIPMIKETLKTVSIMAATSGFLLYNEVFFLTNGAAGTKSISFIIRELAVSSSRTQYAR
                     ANTIGVIQILGGMLIILCINLIFKEGKRRK"
     gene            8752..9591
                     /locus_tag="ACHU66_001083"
     CDS             8752..9591
                     /locus_tag="ACHU66_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR3389375.1"
                     /translation="MDNRLNGLSKSGKLIIYIYMAITCLISVFPILWIFLSSLKADPM
                     KNPGISLPTDLSFDGYIKVFTELHVLDYFWNSFKVVSISVIISIIMISMSSYVIARMD
                     FRGKKLITAMLYSTLFIPATAMTFPVYRLVNVLGIYNTPVALIFVYSCSGIAMSFFII
                     KNYFAIIPKELEEAAEIDGASYAQTFWKVMLPIARPGILTAAILAFINNWNEYYWASM
                     LIINKDELTVPALLGQFTTSFNTNYNGLFSAIVVIILPPILLFAFASKYFIEALGGGA
                     VKG"
     gene            9588..10922
                     /locus_tag="ACHU66_001084"
     CDS             9588..10922
                     /locus_tag="ACHU66_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HFR3389376.1"
                     /translation="MTKNSLQEINKRTEWFQKDRFGMFIHWGLYSIPGKGEWIRSHQQ
                     LSIDDYQPYFEAFNPVKYNPREWAKAAKAAGMKYMVLTAKHHDGFCLFDSAYTDYKST
                     NTQIRKDLVAEFVESVREEGLKVGLYFSLIDWYHPDYPKYGDMIHPMRENEAFKDEKI
                     DFDNYLTFLHNQVEEIVTKYGKLDILWFDYSYGEMFGEKWRATDLIKLVRKHQPDVVV
                     DNRLETSGEGFGSIVTDNINLYSGDFVSPEQIVPHEGIRNIHGEPIPWELCLTMNNNW
                     AYNPTDYLYKSSRTLIRKLVECVSKNGNMILNVGPDALGIINQESLEILNDFGKWLDK
                     NGESIYGCGVSSLPKPEWGYYTQKGNTVYAHVFEQPIGPLALIGIDESKVKRMSFLHD
                     GSEVKISKSWTTNAYEGICFAQYGDIPHFTYPLPDSVDSVIKIELVGDDSED"
     gene            10912..12789
                     /locus_tag="ACHU66_001085"
     CDS             10912..12789
                     /locus_tag="ACHU66_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylgalactosaminidase"
                     /protein_id="HFR3389377.1"
                     /translation="MKIENKQIARYFKLEDGKFYTSHILNKKLDEPIGNEKNTEFLVS
                     FCDGSQLSSEDFTAHVVEQTNESLLVSFYHSEIQLTIFYSKREDVLAKEVTILSSSKT
                     INYIDVEQFGFANLEQVYIPKQQEDIKEMAGFSGYYVELGQPIYAKSFFFGMEFPMGE
                     NRLVDQTYFSRYYVGHKIKEPKKIWPVVIGAASGFEKASIQQEFFTYISGIAQPSYFR
                     KQYNSWYDHMTAIDEGIIVKSFEEISHGFEDNGVKLDAYVVDDGWCDYQSVWEFNEKF
                     PNGLTKVKALVNNLGASLGLWIGPRGGYNGTEVIMSDWLEAHPEFGSKNALSNDVNIG
                     DFNYLEGMKQKMLDYQKKYDISYWKIDGWLLKPDKPDPSGEFAMHTMTPVYEFLIDLL
                     KDLRAERGDRDCWLNLTSYVNPSPWFLQWVNSLWIQISQDVGFTENAGNDIQRMITYR
                     DCQYEEFLAKRDIQLPLWSLYNHEPVYASTAHTWYMDHQMYASVEEFEDYLLFISTRG
                     NAFWEFHYSYTMFDQERWKANARAVKWIENNYSTLKHSQMIGGKPSEFEIYGYRCIDE
                     DVGKEILSLRNPASHSATIQLDNINLSDYQLVRGDYKALTETEYELAPYTILIAEKLG
                     G"
     gene            12791..13684
                     /locus_tag="ACHU66_001086"
     CDS             12791..13684
                     /locus_tag="ACHU66_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFR3389378.1"
                     /translation="MAVKYALGVDIGGTKVAVGLVDGTGHVAYSLKVPSNKESSETLF
                     QCVCTTIRELLNSQQLNIEDVAGIGVGLPGKVDVENGVAVFQNNIPWENFPVVKRLQK
                     EFGNIPIKIDNDVKVAAYAEYRMLSLEADDMFGYITISTGIAATNIINNTILRGSGFA
                     GEIGFMPVRSFGRTSGLEISCSGPAIERQGKQMYGEDLSTKAVFDNWRKGDITASAII
                     ANARDGMVQAIHNMICLLDPKIIVLGGSVAQNNPDFIEDIKTVLGLSLHHEQKHILNN
                     IIISKIDNGNNGIIGSAFLVQ"
     gene            13827..>16588
                     /locus_tag="ACHU66_001087"
     CDS             13827..>16588
                     /locus_tag="ACHU66_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 98 domain-containing
                     protein"
                     /protein_id="HFR3389379.1"
                     /translation="MKNNKRKFTLLTIATLASAFLFQNHVFASEVSLSDLNWTTATHG
                     DATSSKTVQKNKPFTAGNENRENKISLKMEDGSIREFEKGIGTVAANPSTISYDISNL
                     GATKFKSFVGIDRTAVPTDNRYSKISKFEVLVDGAVIYTSIDNYPDGIKYDTSAIWLE
                     LDIPSNAQIIEFKSYSGEHTWGDEVVLGNPTFEIPDSSEIQDDAVDVVDQTSPNELPV
                     RENSVYLSDLDWRSASHGDATASKTIQKNKPFTLGNNGSNQKISLKMSDGSIKEFEKG
                     LGTIAAGPSTIQYDISGAGVSRFTTFVGLDRSAGHADSRYANIEKFEIEVDGTVIYST
                     LNEYPDGFNYDTPAIKVDVEIPEGARSIRLNSYSGQQTWGDELVLAGAYFVASGQFRN
                     PNDFEPAPKRREISNTSPLLMMPLYANGSEYSKGNYSFWGEDTLVGKWENIDPELKPY
                     TVIQVHPDDLPHRAGVAQDFYEKILEQAQNYVNPTTGQNEPIPVILTVYTAGNQSHYT
                     AAHWITMEWIDRMYEKYSCLQGIFSTENYWVWAGNVESNAAEYLKLSARHGGYFIWSE
                     QNNGASIEKALGTQGRPIFKETVEKYWQNFIFMFKNTPAHEGNDAPTVSYMTGLWLAD
                     YAYQWGGLMDTWKWYETGKWKLFESSGIGKTQGNRQWLTQPEAMLGAEAINIYLNGGS
                     VYNFEHPAYTYGVRNEESPLYSTVIENFFKYVINNPAPSKEDVLAATKILINGNFSSK
                     RDGHFYVDVNTATHQSPLYTTGRYGVIPAVPSSISSEVLNSKLPDHIQVVSLNDGSMS
                     SAANRRNLLNGLYPEEYTGNIFGDEIDNRAFIYNYEYNRDNDQTGSFELSGKEFDVTL
                     KSHSFTIVENIATGLNIKQNNFRINKDSLWEGASNSTQARALPQLSKSDAINWVFNTY
                     IHNTPSS"
BASE COUNT         5223 a         2733 c         3499 g         5133 t
ORIGIN      
        1 aaaggtttat cgtaagctaa taattgattt tttgccattt tatatgcctt tatttctttc
       61 tgatattctc ttatatattg ttggttatct ttgttatttt ggtaatgttg gtagatgagt
      121 tggtactgtt gtactatgtg tagactttcc attttgtctt ccaaatcagc catttgttca
      181 tcaatagtct ttacttcatc ttggattgaa atttgtttat cagctaatga ttcaatcttc
      241 atctctaagt catcttttgt gtaaattcct aaatttctta gttcgaccaa accttgtgcc
      301 attgatttta agttgtgttt tctagcccag aactctaatc ctttctggtc tttaaacgta
      361 gacgtatcaa tcagctcatg aggtggtttg gaagcaattc tctcttttaa tctttcttct
      421 gtatagtcag gaccaattgt tttggatcgt gtaaatcgtg aagcattctt gcctttgaaa
      481 ctaatatgtt taccaaattt gatgtcacat ttggattttt tcatacggtc taggaagtct
      541 tcccaagagt ttgaacggga aatatttagg tcaataagaa attgcaagta tggtttccaa
      601 cccttgccat tttgactttg ttcccactca taatgagatt ttcctctagt tttatatttc
      661 ttacggaagg tctgataatg ttcatcaatg acggatagct tgtatttctt gcaaagtcga
      721 tcactagttg accgaatctg gtgataggtt cgtttattgc tttggtaaca ctttccattc
      781 ttgaaattga cattattaaa aatgatatgg ttgtgaatat ggcccttgtc aatgtgtgta
      841 gttaagacat actcgtattc atcttttaag attttttgac atagttccaa gccaatctta
      901 tgtgccgtct cttggtcaac ttctcctggt aaaaaggatt gaataaggtg tcgagccagg
      961 acacttccat ttacttggtg ttcttttcga gtctgtaaaa actgtaaatg agcagtagtt
     1021 gggaagcaca tatttgtgga aagaagtgtt tggctatctg ttttcgtcct atctaggatg
     1081 tagtcaattg caaaatggag agtagactta attgggtgaa ttttagtaac agccatatca
     1141 aatctcccct tgatttgtcc tattgtagag agtgttttgt agtttatgca gttccttact
     1201 caagtgctgg acttcatttt gtattgtcac aatgtcttcc ttgtaaacaa ctgagtaggt
     1261 attgaccttc tttgcaattt ggttgatatt agttgatgtc ttggatagaa gatgttgtat
     1321 ctctctaaaa ggttgcatat caatttcata gattgaagtt tccaggatag acttgagaac
     1381 aaaatgccgc attgattttg accgagattg tccaaatttt tctcgcactt gagcaagttc
     1441 cttgtctgaa agtcgaattt ttaattggtt tggtcgggta cgtttttcca ttgtctattc
     1501 ctttctatcg gggtcttagg gttctcccta acaagtaaaa tataacgaaa aaagcagagc
     1561 caaaggcgtc tgcttcgtta attttatcgt aagtgggtac ccacttactg tgcttgctac
     1621 aacagtttta tattgtagag cggtaagcta agattctatt taatttaatt attggcaatc
     1681 cattctactt catacttttc ggcagaggtt aataaggatt cgtcaggtaa tttgtctgaa
     1741 atgatagttt tgtattgttt caggttgtta attttgaaaa atcctgacat ccgaaatttc
     1801 gaactatcaa tcagtaatat tgtctcttta gccttgtcaa taatagattt ttttgcaatg
     1861 gcttgactga aagaagcttc ataaacatat tcttcatcaa ttccccttgc agaacaaaaa
     1921 gcaaggtcta tactaaaatg ttcaagaaga ctattttcca tactatggtt tacgactgag
     1981 gcagaatggt gtttgacctc accgccagtg acaaatattt tcattgttgg attagaaagt
     2041 tcagaaagca actgagcagt attgagtcca ttcgtaaaga taattagatt gtcaattgtt
     2101 gaaaggaacg gacataattc ataaaccgtt gtacttgaat ctaagtaaat acacatccct
     2161 ggaccaacaa aatcttttgc aagctcagca atgattttct tttctgacct attttcagtc
     2221 tctctgatac tatgagcaag ttcgacggta ttaaatgcgt ttagcaccac ttccccatgt
     2281 tttcttttga tgagaccttg ttcttcaaga tagattaaat ctctacgaag agttgatgta
     2341 cttgagaaag ttaatttttc aagttgtttt acatctattc tctttctact gtgaagaata
     2401 tttgttattt cttttaatcg tttatctcta ctcatactaa cacctcttaa gtacataata
     2461 acatattctg aacaaaaatg gcgattttat gtcaatattt tgaataattt tcaatctact
     2521 gttcaagtat tgtaatagaa gtttgtatat attaaaataa aatcatgaaa gcggtaacag
     2581 aaaggaggtt caaatgaaaa ctgtattggc tattgacctt ggagctacgt cgggaagagg
     2641 aatcctatat tctattgaaa atggaaaact gatttcgaga gaagttcatc gattcaacaa
     2701 ttcaatacaa aaagtgaagg gaagactgtg ttgggatata gaaagcttgg taaaaagtgt
     2761 taaaaaatgt atcagaattt ctcaagaaca gtcagaatta tcttctgttg gaattgatac
     2821 atggggtgtt gattttgcat tattggatgt aaatggagaa atgattgctc aaccagtatc
     2881 ctatcgagat aggcgaactg aatcaatatt agatgagatt gcaagatatt cttctctaaa
     2941 tgatttgtat tttaaaacgg gtaatcagtt gatggagatt aacacactat ttcaattgat
     3001 tgcttgtaaa tctcaaaccc ccgaagaata tttcaaagct gataaactgt taatgatttc
     3061 agattactta aattttgtat tgacaggtga ggtcgcaatt gaaagaagca ttgcttccac
     3121 aatgcaaatg attaacccat tgacacaaga ctggaatcat gaaattttaa aaacatttga
     3181 aatttcagaa ttacttttac cgaaactagt atccgaagga aatatacttg gtagtatatc
     3241 ggacgaaata ttgaataatg aaataacagt aatcaatgtc tgtcagcatg atacagcaag
     3301 tgcggtagca gcgattccac atgaagagga aaacttactc tatgtctctt gtggaacttg
     3361 gtcacttatc gggacagaac tgaaaggccc aatacttacc gatagtgcac tgaagtataa
     3421 tttcacaaat gaagctggct atgatgggac tactagattt ttgaaaaatt gtactggact
     3481 ttggattatt gaagaattaa aaaaatcgtt cttaaaatta ggaaaagatt tttcatacga
     3541 cgaaattaca gagatggtga acaatgtgga aagttgtgtt ccaacatttg acacagatgc
     3601 ttcgacattc gcaagtccag gtaacatgat tgaaaaaatt gtctgttatt tttcagataa
     3661 ggggataaaa gtatcaacag acccagcgat tctatttaag atagtttatc aaagtttggc
     3721 tgaaaaatat aaagaagtca tcatacaatt ggaagagata acagggaaca tctataagag
     3781 aattgactta ataggcggtg gttccaagtc agaatatttc tcacaattag tagcagatgt
     3841 tacaggtaag acggtggtta ctggtctgta tgaagcaacc tcaatcggta atgccctcgt
     3901 tcaatttaag gcacttggct atgttgaaga tatgaaagaa gccaagaaac ttgtaaaaga
     3961 gtctataaca tttaaccatt tttatccaaa gaaggaggaa aaaaatgaca ctttatccta
     4021 aaattggaat ccgtccgact attgatggtc gacagggcgg tgtaagggaa tccttagaag
     4081 aaaaaaccat gcaaatggct atggctgcta aggatttgat tgagtccaat ttgcgttacg
     4141 cagacggaac ccctgttcaa tgtttgctgg ccagtcgaac cattggtggt caaggggatg
     4201 cagggattgt ctatgatgag tttgctcgtg acaatgtagt tgcaacctta tctgtcacac
     4261 caagttggtg ctatggaaca gaaaccatgg atatggatcc acatacgatt aaagctattt
     4321 ggggctttaa tgggacagag cggcctggag cagtttatct agccgcagcc atgagtggct
     4381 acgctcaaaa gggttaccca gcattcaaga tttatggtca tgatgttcag gatctggatg
     4441 acaatagcat tcctgctgat gtagctgaaa aaatcttgct atttgccaag ggagccattg
     4501 ctgctggtca gatgaaaggc aagtcctatg tcaacattgg agcttcttca atgggaattg
     4561 cgggttctca ggttaccact tccttctttg aagactatct gggaatgttg gtcgaatttg
     4621 tggacatgac tgagattctt cgccgtatgg agttggaaat ctacgataaa gaagaatatg
     4681 aaaaagctct tgcttggatt aaggaatatt gtaaagaggg tgttgacatc aatgcaggaa
     4741 aggaattccc agaagtgatt accaagtcca aggtgattcc agctgacaag gattgggaat
     4801 ttattgccaa gcaaaccttg attatccgcg atattctctt tggaaatcct cgcttggcag
     4861 agattggctg ggaagaggaa gctcgtggca gaaatgcaat ttcaggtggt ttccaaggcc
     4921 aacggcaatg gactgactgg ctaccaaatg gtgattttgc tgaagcgatt ctggcttcca
     4981 cttttgactg gaatggttca cgtcctgtga ctgcctttgc aacagaaaat gatactttga
     5041 atgcgaccgc catgttattt ggaaccctcc taaccaataa ggctccaatt ttctctgatg
     5101 ttcggacctg ttggagccca gaggcggtca aacgagtgac tggtcaagag ctaacaggta
     5161 gggctaagga tggcatcctc caccttatta actcaggagc ttcagccctt gatggaagtg
     5221 cagcagcacg agatgaagct ggtcagccaa ccatgaaaga gttctggaat atgacagagg
     5281 aggatataaa agcctgtctg gaagcgacag attggtgtcg agcaaactat gaatatttcc
     5341 gaggaggtgg ctattcttct cacttcaaga ctcaggggga acttcctgtg accttgattc
     5401 gtgtcaatct agtcaagggt gttggtccaa gtttacaaat tgcagaaggc tacacctgtc
     5461 cattggagga tgaagtgcac cggatcttgg atgaacggac agataaaacc tggccaacaa
     5521 cctggtttgc tccgaatctt ggagaacctg gttttgaaac cgtctatgat gtcatgaacc
     5581 attggggttc taaccacggt gcctttgttc atgggcacat tggtgcagaa gttatcactc
     5641 tggctagtat gttgcgaatt cctgtagccc tccacaatat tccaagggaa cgaattttcc
     5701 gtccaagcat ttttgatggt gtcggtacta aggatttgga agcggcagat ttcaagatgt
     5761 gtgaggtttt aggaccgcta tatagtaaat agaaggagaa cactttatgt tcatggaaaa
     5821 agaaagaaag gaacttataa agtacggcaa aaagttagtt actgaaggct taaccaaagg
     5881 tactggtggg aatctgagcg tatttaaccg tgaaaaaggt ctaatggcaa tcactccatc
     5941 tggaattgat ttctttgaaa tcaaggaaga agatatcgtc atcatggact tggaaggaca
     6001 agtagtcgaa ggtgaaaagc ttccttctag tgagtggtat atgcacctga ttcaatacca
     6061 gcaacgagaa gaccttgatg cagtcattca tgcccatacc acttatggaa ctgttttggc
     6121 agtactaaga gagccgttac cagctagcca ttatatgatt gcggtagctg gtaaagatgt
     6181 ccgagtagca gaatatgcca catatgggac taaggaattg gctgaaaatg ctgcggaggc
     6241 tatgaaagac agacgtgcag tctttcttgc caaccacggt atcttggctg gtgctcaaga
     6301 cttactaaat gccttcaata tcattgagga ggttgaatac tgttcaaaaa tttattgtgt
     6361 tgctaaaagc atgggtgaac cagtcatcct cccagatgaa gaaatggaat tgatggctga
     6421 aaaatttaaa acttacggac aaaggaaata aaacggagga acataaaatg aaaaaaggtt
     6481 tgttgaaatg cggagcattg tgtttatttg cttcagtgat tctagctgct tgcggatcgt
     6541 catcaggaac tagttcaagt tctgaaaaag cagcaagtgg tgatgataca gttttagagt
     6601 tctatcatgg ctatcaccat agcgaagaag aatggccagc tgctaaagtt atgcgtgatc
     6661 tttatgatga atttgcgaaa aaacatgcaa ccggtgatgt tgaatttaaa ccaactcctg
     6721 tgaacggaag cttgacagac attatgaata acaaggttgc aagtggcgag tttccagata
     6781 tgattgactt ggctggtaac gatgtttcgc tagcagctat tgaacaaagt ttggttctgg
     6841 atttgaaacc atatatcgat gataatggat tggaaaaaaa tgttggtcta aactatacac
     6901 aaaatgatgt gaacggaaaa atctatacag tccacgacca attattaacc ttaggtcttt
     6961 ggtacaatgc agatattttc tcaaaagctg gggctaaaac tcctgaccaa tggaccaagt
     7021 ggagtgattt tactgaagct atggccttgg ttcgtaaaca aggagtatat gcctttggtg
     7081 caggagagcc ggcaattcgt ctctttaaca ctgtacttgg ctcatctgaa gctggccgta
     7141 aactattaga aggtcctcta acttctgaag gaattgattc aaaagaattt gcagaagcac
     7201 ttaaattggt tatgacagaa gtgcaagcaa acggttcagc taatgcaggc ggcgatgcca
     7261 acgtttattc aactgatttt actgaaaata aatcagcagt cttcttcaac ggtgtatggg
     7321 ctgcaggtgg tttgaaagat aatgcagctt tgaaaccagg attgtacgct ggtaatgttg
     7381 cgattagttc agctggtggc ggtattacaa tttcaagtaa actatctgag gaagaagaaa
     7441 aacttgcatt ggaatttgta aaatacatga ccagtgacga ggttcaaact acaattttcg
     7501 aaaaagtggg agcgaaccca gctagccaag cgattgatac tgctcaaatt gcaagcggtt
     7561 ctgacgacgc tactgtaaaa ctcttaggtg aagcgacatc tatggcaaac aatgcagatg
     7621 taactgttcc tactgttgta tctgtttggg gtggtgacgt tcgtactgca attatcaacg
     7681 cattgaccga aagtgctgct gacggtgtca atatcgatca gaaagtaaaa gaaacacaag
     7741 acatcttgaa agcccttatc ggctaaaata acagggctaa ggatgtattc cttagccctt
     7801 ttttagaaag tgagtatttt atggcaaaga aaaccatgac cttacaaaat aggaaggcac
     7861 agaaacaacg atttattttt ctatttttac tcccgacatt agtttgtttc tttctcttct
     7921 acttttattc ggtggtaact atttttgcaa catcctttac taagtgggat tatacgaatt
     7981 taaccagccc agaatttcta ggttttggca atctttttgc caactataag tacattttta
     8041 gtgaatatcc tttctttttg gaagcactga aaaattcaat tacatgggca attataggtg
     8101 tagtagttca agtcccatta gctttatcag ttgctattgc cttatcaaaa aaattggctg
     8161 gttggaaact atctcgaaac ctttatatca tacctagtat tatttctagt gccgcaatgg
     8221 gtttgatttt tcttcaaatc tacaatccaa attatggagt agttaaccaa gttatccgct
     8281 tttttaaccc tgaattttcg gatgcgattt tattgacacc tggcttgaat attattgcga
     8341 tgacttgtgc ttatattttc tttgcaggga catcgacaat aatgatattg ggtcaaatct
     8401 tcgccattcc acaagagatc catgaagcag caatactaga taacattaca gggtggagaa
     8461 gagaatggta tatcaccatt ccaatgataa aagaaacatt aaagacagtt tcgattatgg
     8521 ccgcaacttc gggtttccta ttgtataacg aagtattctt cctaacaaat ggtgcagctg
     8581 gaacaaaaag tatcagtttt atcataaggg agttagcggt atctagttct cgtacacaat
     8641 atgccagagc taacactata ggtgtgattc agattttagg cggaatgcta atcattttgt
     8701 gtatcaatct gatattcaaa gaaggtaaac gtagaaagtg aggtagagca tatggacaat
     8761 cgtttgaatg gcctttctaa gtcagggaaa ttaattattt acatctatat ggctattact
     8821 tgtttaattt cagtgtttcc aattttatgg atatttctat cctcattaaa agcggatcct
     8881 atgaaaaatc caggcattag tttgccaact gatttaagtt ttgatggtta tatcaaggtt
     8941 tttactgagt tacatgtatt ggactatttt tggaacagtt ttaaagttgt ttccatatct
     9001 gtgattatca gtattatcat gatttccatg tcatcttatg ttattgctcg gatggatttc
     9061 cgtgggaaaa agctgataac agcaatgttg tactccactc tatttatccc tgcaacagct
     9121 atgactttcc ctgtctatcg tttggtaaat gttttgggga tatataatac gccagtagct
     9181 ctgatttttg tctactcttg tagcggtatt gccatgagtt tctttatcat aaagaattac
     9241 tttgcaataa tacctaagga gttggaagaa gctgctgaaa ttgatggagc gagctatgcc
     9301 cagacatttt ggaaagtaat gttacctatt gctagacctg gtattttaac agcagcaatt
     9361 ttagctttta taaataactg gaatgaatac tattgggcat caatgttgat catcaataaa
     9421 gatgagctga cggttccagc cttgcttggt caattcacaa ctagctttaa tactaactat
     9481 aatggtctct tttcagcaat tgttgtgatt atcttaccac caattttact atttgcattt
     9541 gcaagtaaat actttattga agcccttgga ggaggagcag taaaaggatg acaaaaaata
     9601 gtttacaaga aattaacaaa cgtacagagt ggttccaaaa agaccgcttt ggaatgttca
     9661 ttcactgggg gctctattca atccctggaa aaggggagtg gatccgcagt catcaacaat
     9721 tatccattga tgactatcag ccatattttg aagcatttaa tcctgtaaag tacaatccga
     9781 gagagtgggc gaaggcagca aaagcagcag gtatgaaata tatggtcttg accgccaagc
     9841 accatgatgg attttgcttg tttgactcag cctatactga ctacaaatct acaaatacac
     9901 aaatcaggaa agacttagta gctgaatttg ttgaatcagt acgagaagaa ggattaaaag
     9961 taggtctata cttcagtctg atagactggt atcatccaga ttatccgaaa tatggcgata
    10021 tgattcatcc tatgcgtgag aatgaagcct ttaaagatga gaagattgac tttgacaact
    10081 acttgacttt cttacacaat caagtggaag aaatcgtaac caagtatggc aaactggata
    10141 ttctatggtt tgactattct tacggagaaa tgttcggcga gaaatggcgt gcaacagatt
    10201 tgattaagct tgtacgtaag catcaaccag atgtagttgt tgacaatcgt ttagaaacat
    10261 caggagaagg atttggcagt attgtaacgg ataatatcaa tctttattct ggagactttg
    10321 tcagtccaga gcagattgtt ccgcatgagg gaattcgaaa tatacatgga gagccaattc
    10381 cttgggagct ttgcttaact atgaataata actgggccta caatcctacg gattatctct
    10441 acaaatcgtc caggacactt attcgtaagt tggttgagtg tgtaagtaaa aatgggaata
    10501 tgattctgaa tgtaggtcca gatgcactag gaattatcaa ccaagagagt ctggaaattc
    10561 taaatgattt tggaaaatgg ctggataaga atggggagtc catttatggt tgtggcgtta
    10621 gttcattacc taagccagaa tggggctact atactcaaaa agggaatacg gtttatgccc
    10681 atgtcttcga acagccaatt ggtccacttg ccttgattgg tatcgatgaa agcaaagtga
    10741 aaaggatgag tttccttcat gatggtagtg aagtgaagat ttctaagagc tggacaacta
    10801 atgcttacga agggatttgt tttgcccagt acggcgatat tcctcacttt acttatcctt
    10861 tgccagacag cgtagatagt gtgattaaga ttgaattagt aggtgatgat agtgaagatt
    10921 gaaaataaac agatagcacg ctattttaaa ttagaagatg ggaagtttta tacttcccat
    10981 atcttgaata aaaagcttga tgaacccatt ggaaatgaaa aaaatacgga attccttgtt
    11041 tctttctgtg atggaagcca gctcagctct gaggatttta cagctcatgt ggtcgaacaa
    11101 accaatgaaa gtcttttggt tagtttctat cattcagaaa tccagttaac cattttttat
    11161 tcaaaacgtg aagatgtact tgctaaagag gttactattc tgtcgtcttc taaaacaatt
    11221 aactacattg atgtggagca gtttggtttt gcaaacttag agcaggtata tattccgaaa
    11281 cagcaagaag atattaagga aatggctgga ttttctggct attatgtaga gttgggacaa
    11341 ccaatttatg ccaagtcctt cttctttggt atggagtttc caatgggaga aaatagactg
    11401 gtagatcaaa cctatttttc tcgctactat gtcggtcaca aaattaaaga gcctaaaaaa
    11461 atctggccag ttgttattgg tgcggcttct ggatttgaga aagcaagcat tcaacaggaa
    11521 ttctttactt acattagtgg aattgctcag ccaagttact tcagaaaaca atataattct
    11581 tggtatgacc atatgactgc cattgatgaa gggattattg taaagagttt tgaagagatt
    11641 tcacatggct ttgaagacaa tggtgtgaag ttggatgcct atgtcgttga tgatggctgg
    11701 tgtgattacc aaagtgtttg ggagtttaat gaaaagttcc ctaacggatt aacaaaggta
    11761 aaagctctcg taaataattt aggtgcaagt cttggtcttt ggattggacc acgtggaggc
    11821 tataacggta cagaagttat catgagtgac tggttggagg cacatccaga attcggtagc
    11881 aagaatgcct tgtcaaatga tgtcaatatt ggtgacttca attatttgga aggcatgaag
    11941 caaaaaatgt tggattacca gaaaaagtat gatatcagct attggaaaat tgatggttgg
    12001 ttgctgaagc cagacaaacc agaccctagt ggcgagtttg ccatgcacac tatgactcct
    12061 gtctatgaat tcttgattga cctcttaaaa gatttaaggg cagagcgagg agatagagat
    12121 tgctggttga acttgacttc ttatgtcaat ccaagcccat ggttcttaca atgggtcaac
    12181 agcctttgga tacaaatttc gcaggatgtt ggctttacag aaaatgctgg gaatgatatt
    12241 caacgaatga ttacctaccg tgattgccaa tatgaagaat ttttagctaa acgggatatt
    12301 caattgccac tttggagtct ttataaccat gaacctgtct atgcgagcac ggctcatacc
    12361 tggtacatgg accatcaaat gtatgcaagt gtagaagagt ttgaagatta tctcctattt
    12421 atttccactc gaggaaatgc tttttgggag tttcattatt cctatacaat gtttgatcag
    12481 gaacgttgga aggccaatgc tcgtgctgtc aaatggatag agaacaatta ctcaacttta
    12541 aaacatagtc aaatgattgg tggcaaaccg tcagagtttg aaatttatgg ttacagatgt
    12601 attgatgaag acgtaggaaa agaaattctt tcgcttcgga acccagctag tcattcagcg
    12661 actattcagt tagataatat caatctttcc gactatcaat tggttagagg agattataaa
    12721 gctctgacag aaacagagta tgaactagct ccatatacaa ttctgattgc agagaaacta
    12781 ggagggtaat atggcagtta aatatgcttt aggtgtagat attggtggta caaaagttgc
    12841 tgtgggactg gtagatggta caggtcatgt ggcttatagt ttaaaagtgc caagcaataa
    12901 agaatcatca gaaaccttat ttcaatgcgt ttgtacaact attcgagaac tactaaacag
    12961 tcagcaactg aatattgagg atgttgcagg gattggagtt ggcttgccgg gaaaagttga
    13021 tgttgaaaat ggagttgcag tttttcaaaa caacattcca tgggagaatt ttcctgttgt
    13081 gaagcgactt caaaaagaat ttggaaatat tccaataaaa attgacaatg atgtgaaagt
    13141 agcggcctat gcagagtatc gaatgctttc attagaagca gatgatatgt ttggctatat
    13201 cactatctct acaggtatcg ctgctaccaa tatcataaat aacacaattc taagaggctc
    13261 tggctttgcg ggcgaaatcg gttttatgcc agttcggagt ttcggaagaa caagtggtct
    13321 agaaatctcc tgctcaggac cagccattga acgccaaggg aagcaaatgt atggtgaaga
    13381 tttgtcaacc aaggcagtgt ttgataattg gcgtaaagga gatattacag catcggctat
    13441 tatcgctaat gcgagagatg gcatggtcca agcgattcat aatatgattt gtttactgga
    13501 tcctaaaata atcgttttgg gtggtagtgt tgcacaaaat aacccagatt ttattgaaga
    13561 tataaagacc gttttgggat tatctttaca tcatgaacaa aaacatattt tgaataatat
    13621 tatcatctca aaaatagata atggaaataa cggaataatt ggttctgcat tcctagtcca
    13681 ataatagaga agaaatatga tgaaaataga tagttcataa tactttcaat tttatagttt
    13741 agtttgtttt ccaatatcag aaaaaatgga ctaggaaatg ttgggaagca aaagttctct
    13801 tttatcaaga ataagaggta aaacaagtga aaaataacaa gagaaaattt acacttttaa
    13861 cgattgcaac attggcttca gcattcttgt ttcaaaatca tgtgtttgca tctgaggttt
    13921 cattgtctga tttaaattgg actaccgcaa ctcacggtga tgctacttca tccaaaactg
    13981 ttcaaaagaa taaaccattt actgcaggga atgaaaaccg tgagaataaa atttcattaa
    14041 aaatggaaga tggctcaatt agagaatttg agaaaggaat cggaactgtt gcggcaaatc
    14101 cttctacaat tagctatgac atttctaacc taggagcaac gaaatttaaa tcctttgtag
    14161 gaattgatag gacagctgtt cctacggata atagatattc caaaattagt aaatttgaag
    14221 tgcttgtaga tggggctgta atttatacat caattgacaa ttatccagat gggattaaat
    14281 acgatacatc agcgatttgg cttgaattag atattccatc taatgcacaa ataattgaat
    14341 ttaagagtta ctcaggagaa catacatggg gtgatgaagt tgtgttaggt aatccaactt
    14401 ttgaaattcc cgatagttcg gaaattcaag atgatgctgt agatgttgta gatcagactt
    14461 ctccaaatga actacctgtt agagagaatt ctgtttatct ttcagactta gattggcgaa
    14521 gtgctagtca cggtgatgcc acagcttcaa aaactattca aaaaaataaa ccatttacgc
    14581 tagggaataa tggaagtaat cagaaaattt ctttaaaaat gtctgatgga tctattaagg
    14641 agtttgaaaa aggactaggt acaatcgctg ctggaccatc gacaatacaa tatgatattt
    14701 ctggagcagg agtatcacgg tttaccacat ttgtaggatt agataggagt gctggtcatg
    14761 cagatagtag gtatgctaat atagaaaaat ttgaaattga agttgatggt actgtaattt
    14821 actcgacttt gaacgaatat ccagatggtt ttaattatga tactccagca attaaagttg
    14881 atgttgaaat tccagaaggt gctcgttcta ttcgtttaaa tagttattct ggtcaacaaa
    14941 cctggggcga tgagttagtt ttagcaggtg cttactttgt agcaagtgga caattccgta
    15001 atcccaatga ctttgagcct gcaccaaaac gtagagaaat ttctaacaca agtccattac
    15061 ttatgatgcc attatatgct aatggttcag aatacagtaa aggaaattat tcattctggg
    15121 gagaggatac tttagttggt aaatgggaaa atattgatcc agaattaaag ccttatacag
    15181 taattcaagt tcacccagat gatttgccac atagagcagg agtagctcag gatttttatg
    15241 agaaaattct cgaacaagct caaaactatg tgaatccaac caccggtcaa aatgaaccta
    15301 ttccagttat cttaactgta tacacagcag gcaaccaatc acattatact gctgcacatt
    15361 ggattactat ggaatggatt gatagaatgt atgaaaagta ttcttgtctt caaggaatct
    15421 tttcaacaga aaattattgg gtctgggctg gaaatgttga aagtaatgcg gctgagtatt
    15481 taaaattgtc agcaagacac ggaggatatt tcatttggtc tgagcaaaac aatggtgcct
    15541 ccattgaaaa agctttagga actcagggaa gaccgatctt taaagagaca gtcgaaaaat
    15601 attggcaaaa ctttattttc atgttcaaga atacccctgc acatgaaggt aatgacgctc
    15661 caactgtaag ttacatgaca ggcctatggc ttgctgacta tgcataccag tggggtggat
    15721 tgatggacac ttggaaatgg tatgaaacag gaaaatggaa gttgtttgaa agctctggaa
    15781 ttggaaaaac acaagggaat agacaatggt taactcaacc agaagccatg ttaggagcag
    15841 aggctattaa tatctattta aatggtggat cggtatataa ttttgagcat cctgcctata
    15901 cctatggtgt aagaaatgag gaatcaccat tatattcaac tgtaattgaa aacttcttca
    15961 aatatgtaat aaataatcct gcaccttcta aagaagatgt actcgctgct acaaaaattt
    16021 tgataaatgg aaatttctca tctaaacgag atggtcattt ctatgttgat gtcaatacag
    16081 caacacacca aagtccacta tatacaactg gtcgatatgg tgtaatccct gctgtaccat
    16141 ctagtatttc ttcagaagta ttgaattcaa aattacctga ccatattcag gttgtcagtc
    16201 taaatgacgg cagtatgtct tcggcggcta acagaagaaa tttattaaac ggcctatatc
    16261 cagaagagta tactggaaac atttttggtg atgagattga caatagagca tttatctata
    16321 attatgagta taatcgcgac aatgaccaga ctggttcatt cgaacttagt ggtaaagagt
    16381 ttgatgtaac tttgaaatct cactcgttca cgattgtaga gaatattgca acaggattaa
    16441 atatcaaaca aaataacttc cgaatcaata aagattcatt atgggaaggt gcttcaaatt
    16501 caacacaagc tagagcatta cctcaactat caaaatctga tgctataaat tgggtattta
    16561 atacttatat ccataatact ccaagctc
//
LOCUS       DAZQCJ010000036        16552 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000036 DAZQCJ010000000
VERSION     DAZQCJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16552
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.036"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(22..924)
                     /locus_tag="ACHU66_001088"
     CDS             complement(22..924)
                     /locus_tag="ACHU66_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR3389380.1"
                     /translation="MSKENKKQVQSASTPPGGFRVIAREFMKDRLALTSLIILVTVLL
                     AVFIGALLLNQEQVMKVNLLGRYLEPGVDGYILGTDEGGKDIFGQLIIGARNSIIIGF
                     TITIITSIVGISVGLISGYYGGRLDGFLMRIVDFIMILPVTMLIIVFVTVIKNYTIYH
                     FILIMSAFYWTAKARLFRTRTLSEASLDYVNASKTLGTSDFMIMFREILPNLSSLIIV
                     NLTLNFAGNIGIETSLTYLGFGLPSTVPSLGTLIANARNADILENKTWIWLPAAIFIL
                     VMMLCINYVGQAFQRAADAKQRLG"
     gene            complement(938..1894)
                     /gene="opp4B"
                     /locus_tag="ACHU66_001089"
     CDS             complement(938..1894)
                     /gene="opp4B"
                     /locus_tag="ACHU66_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFR3389381.1"
                     /translation="MWKTVLRRLLMMIPQIIILSVIAFFVAKMMPGDPFTGLIDPNID
                     PAIIEQKRIAAGYYDPIPIQYFRWVGNLLQGDFGQSVIFKQPVVDVIMQRLNNTIGLS
                     LLTMVLTYLIALPLGMIAGRYQNSLADKIIGVYNFLTFSTPTFVFAILMLWMFGFSLG
                     WFPTRGSISGGVEGFAATLSRLHHMILPAITMAILSTTVTIQYLRTGIIDAKSQDYVR
                     TARAKGVPERVVYNRHIFRNSILPIASFLGYELTGLIGGSIFIENIFTYPGIGQLFFN
                     SISSRDYSVILALLLVFGMGTLLGTLISDIIMSIVDPRIRVK"
     gene            complement(1896..2837)
                     /locus_tag="ACHU66_001090"
     CDS             complement(1896..2837)
                     /locus_tag="ACHU66_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR3389382.1"
                     /translation="MGFIEVKDLKVHYPIRSGFFNRVTDHVYAVDGVNIEFEEGKTYG
                     LVGESGSGKSTIGKAIIGLERATSGQIIYEGQNVTNKSRGKKGNFNRDVQMIFQDSLS
                     SFNPKKRILDIIAEPIRNFDRLSPDEEKKKVLQLLDTIGLNEEALIKYPHEFSGGQRQ
                     RIGVARALASNPRLIIADEPVSALDLSVQAQVLNYMKRIQDEFKLSYLFISHDLGVVQ
                     HMCDELFIMYRGRFVETGNKNDIYNNPQHIYTKRLLSAIPTIDPLNRLKNKEKRLAAE
                     KEYQEMQGQYYDENGRVYDLRSFSETHQVALPEGGQN"
     gene            complement(2840..3838)
                     /locus_tag="ACHU66_001091"
     CDS             complement(2840..3838)
                     /locus_tag="ACHU66_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3389383.1"
                     /translation="MATEKPLLDIKDLHVGFRIGDDYFDAVDGVDISLQKNEILAIVG
                     ESGCGKSTLATTIMGLHNPLNTKITGSIQYNDMELIGMNEAKYNTVRGNDIGMIFQDP
                     LASLNPLMTIGAQIDEALFYHTDLDANARTERVLELLAQVGIPNPKRTFKQYPHELSG
                     GMRQRIIIAMALSCKPPIIIADEPTTALDVTIQAQILDLLNDIQAETGSGIILITHDL
                     GVVAETADRVAVMYGGQFVEVAPVEELFTNPKHPYTRSLLKSNPQSGSEGGDLHVIEG
                     IVPPITKMVRKGCRFAPRIPWIGAEAHEENPSMHEVGPNHFVRCTCHETFYFEGEA"
     gene            complement(4215..6005)
                     /locus_tag="ACHU66_001092"
     CDS             complement(4215..6005)
                     /locus_tag="ACHU66_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR3389384.1"
                     /translation="MKKTTKLFALAGVTLLSASVLAACGSKQSGAAKQELSFPAEVKQ
                     DGTAVSDAQLKYAWVSPTTSSGLLIDELTENTTDSTFGGFVDISMFGYDGERKLDDSG
                     LAKAEFDVKGKKITISLTGKDYKWSDGEPFTINDYIFTIKSMASKDYTGIRFDDKFLN
                     IEGMQEFVDGKASDISGIKKVDDYTVELTVKEMSPSMMYAGGDVPAYIQPEHIYKDIP
                     VADWEKSEYSRTAKLVGMGPWKIKEIVNGESITYVPNEYFFKGTKPKTSSLKIDIVSP
                     DTIVSEMKAGNYDIAEMPVDQLDSYKDVSNLNIVGQLESSYEYISFNLGKYDEAANKN
                     VMDEKAKMNDVKLRQAIAYAIDTKTAGKSLYNGLYHPAKSLIISFFGDIHDSELEGYS
                     YNPEKAKKLLDEAGYKDVDGDGIREGKDGKAFKITFAARKRTEANEALVQQYIAWWKE
                     VGLNVELYTGRTVEGKSFYNSVQANDPAIDMYAGGWSTGYDPNPSGLWGSVAAFNMSR
                     FVSDENTKLLEAISSAESFDDKKNVENYKAWQKYAHEQAFAIPTFESESIVALNKRVK
                     NYDTNYGSASGKGIALENIELTADKGVVAE"
     gene            6218..7459
                     /gene="pbp3"
                     /locus_tag="ACHU66_001093"
     CDS             6218..7459
                     /gene="pbp3"
                     /locus_tag="ACHU66_001093"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase PBP3"
                     /protein_id="HFR3389385.1"
                     /translation="MRKFLLTLTTVIALFFTSTPILAEDFSVAAKHAIAVEVSSGKIL
                     YEQDAETKASIASISKTLSIYLVYEALAKGEITLDTMVDISDYPYSLTSNTELSNVNL
                     DARSYSVRDLLNASLITSSNSAIIALAEKIAGSEAAFVDRMKAKVQEWGITDTKIVNV
                     SGLDNTDLGENIYPGSSPEDVNMFSALDVAIIARRLILDYPQVLDITSLNAYDFGGYT
                     YYSTNQMLSDGTHARGGVDGLKTGTSNSAGSSFLATTQQAGMRIITVVLNANDGLTSP
                     ENRFVATNDLMNYVYSNFSLVTLVKKGEAFEDSKIKVFNGEKETSPVVAAADLTVVQR
                     KQTDNVPTATFTTDIKEIDAPLKAGTLVSKLQLKDQDLIGKGYISEQASVDMIIPSDM
                     KEASWPFSWWNQFVRYVNEKL"
     gene            complement(7733..9148)
                     /gene="sufB"
                     /locus_tag="ACHU66_001094"
     CDS             complement(7733..9148)
                     /gene="sufB"
                     /locus_tag="ACHU66_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR3389386.1"
                     /translation="MSEERIEPTPIDLGEYKFGFHDENVELVASTGKGLNEEVIREMS
                     RIKGEPEWMLEFRLKSYETFKKMPMQTWGADLSELDFDDIVYYQKPSDKPARSWDDVP
                     DKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEYDKHGIIFTDTDTALKEHP
                     ELFKKYFGKLVPPSDNKLAALNSAVWSGGTFIYVPKGVKLDIPLQTYFRINNEGTGQF
                     ERTLIIVDEGASVHYVEGCTAPTYSTASLHAAIVEIIAHEGAYMRYTTIQNWSDNVYN
                     LVTKRARAEKNATVEWIDGNLGAKTTMKYPAVYLEGEGARGTMLSIAFANKGQVQDTG
                     AKMIHNAPRTSSSIVSKSIARGGGEVNYRGQVTFAKNSAKSISHIECDTIIMDDISKS
                     DTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKEL
                     PMEYAVELNRLIAYEMEGSVG"
     gene            complement(9498..9938)
                     /gene="sufU"
                     /locus_tag="ACHU66_001095"
     CDS             complement(9498..9938)
                     /gene="sufU"
                     /locus_tag="ACHU66_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFR3389387.1"
                     /translation="MALSRLDSLYMAVVSEHSKLPRHRGSLDGVEKLELHNPTCGDVI
                     ELSVKIEKDVITDIAFDGVGCTISTASASMMTEAVLGKEISQIQELAEIFSQMVQGQE
                     DPRQKGLGDASLLAGVAKFPQRIKCATLPWNALKKALERSDSAE"
     gene            complement(9925..11151)
                     /locus_tag="ACHU66_001096"
     CDS             complement(9925..11151)
                     /locus_tag="ACHU66_001096"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR3389388.1"
                     /translation="MIDFESIRKDFSILDQVVNDEPLVYLDNAATTQKPQQVLDVLED
                     YYQTDNANVHRGVHTLSERATARYEAARQKVADFIQAKSSKEILFTRGTTTSLNWVAQ
                     FAKEILQPDQEVIISVQEHHSNIIPWQQACQQIGAKLRYVPLKDGELDVDSLKSILSE
                     KTKFVSLAHVSNVLGGVVPIGEIAELVHQVGAYLVVDGAQSVPHMAVNVQELDVDFFA
                     FSGHKMLGPTGIGVLYGKEELLNLMSPVEFGGEMIDFVYEQSATWKELPWKFEAGTPN
                     IAGAIGLGAAVDYLTEIGMDAIQAHEAELVDYVFPKLQAIPGLTIYGSQDLSKRTGVI
                     AFNLDDLHPHDVATALDYEGVAVRAGHHCAQPLLRYLQVPATVRASFYIYNTKADCDK
                     LVEAIIKTKEFFNGPI"
     gene            complement(11153..12415)
                     /gene="sufD"
                     /locus_tag="ACHU66_001097"
     CDS             complement(11153..12415)
                     /gene="sufD"
                     /locus_tag="ACHU66_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR3389389.1"
                     /translation="MTKEKIQEFSALQAEPTWLTDLRLKAFDKIAELDLPVIERVKFH
                     RWNLGDGSLATNDEIGAVPDFTAIGDNPMLVQVGSQTILEQLPVDLVEKGVVFTDFAS
                     AMDLIPDVIEKYLGVAVAYDEHKLAAYNTAYFNATAVLYVPDNVEIDQPVEALFYQDS
                     TSPVAFNKRVLIIAGKNAKLNYLERFESLGDGAATTSANIVVEVIALAGSQIKFAAID
                     RLGKHLDTYLNRRGYLGNDATIDWAIGLLNEGNVVADLDSELKGNGSHANLKVVGLSS
                     GRQVQGVDTRVTNYGHNSVGHILQHGVILESGTLTFNGIGHIVRGAKGADAQQESRVL
                     MLSDKARSDANPILLIDENEVTAGHAASIGQVDPEDMYYLMSRGLDQATAERLVVRGF
                     LGAVITEIPVKEVRDQLIAVIEEKLTKR"
     gene            complement(12432..13205)
                     /gene="sufC"
                     /locus_tag="ACHU66_001098"
     CDS             complement(12432..13205)
                     /gene="sufC"
                     /locus_tag="ACHU66_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR3389390.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPSYEVTQGEILFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDEDKISVRDFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGINAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAQRLEKEGYVQVAAELGIDYKEDDI"
     gene            complement(13685..14857)
                     /locus_tag="ACHU66_001099"
     CDS             complement(13685..14857)
                     /locus_tag="ACHU66_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR3389391.1"
                     /translation="MIPFALKYIMVIITTMVISAIATPLVRFMSLRVGAVDNPNARRI
                     NKVPMPSAGGLAIFVAFTISVLWFLPGIVTRVNYHGTYLHYIWPLVLASSIIVLTGFI
                     DDVKELKPAPKMFGILAAATIIWIFTEFRFDSFKIPFGGPFLQFPVWLSFLLTVLWIV
                     SITNAVNLIDGLDGLVSGVSIISLTTMGIVSYFFLHDSNIFLTLTIFVLVAAIVGFLP
                     YNYNPAIIYLGDTGALFIGFMIGVLSLQGLKNSTAVAVVTPMIILGVPITDTVVAIIR
                     RKLSGQKIYEADKMHLHHRLLSLGLTHRGTVLVIYTISFLFSLTSLLLNVSSRIGGIL
                     LLVTMGLGIEILCELIGIFGINRTPLLNLLRFIGNSSYRQEKIAKYKKCCEEKKRK"
     gene            complement(14854..15576)
                     /gene="mecA"
                     /locus_tag="ACHU66_001100"
     CDS             complement(14854..15576)
                     /gene="mecA"
                     /locus_tag="ACHU66_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFR3389392.1"
                     /translation="MKVKQISDSTLKITIKLDDLEERGMELSDFLIPQEKTEEFFYTV
                     LDELDLPMTFRESGMLSFRVTPKPDRVDIFVTKSDLDQNLNFDEFTDLSELGDVASMT
                     PDEFFKSLEQTVREKSAPDATAVRHLEEVEQEEEADEEEQERYIYYILEFPTIEDLFT
                     FVGTVDYPVEESELYKMDGHYYLTVLINVENRSKQYPGYILSRMLEFTNDTKLTRPAL
                     QEHGTLMLPLAAIEELRKVPTV"
     gene            complement(15704..16540)
                     /locus_tag="ACHU66_001101"
     CDS             complement(15704..16540)
                     /locus_tag="ACHU66_001101"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR3389393.1"
                     /translation="MLLELLKAIFLGIIEGVTEWLPVSSTGHLILVQEFVKLNQSKNF
                     LEMFNIVIQLGAILAVMTIYFKKLNPFQPGKTKRDIQLTWQLWAKVVIACIPSILIAV
                     PLDNWFEAHFNFMVPIAIALIVYGIAFIWIENRNSGVEPQVTDLAKMSYKTALLIGCF
                     QVLSIVPGTSRSGATILGAIILGTSRSVAADFTFFLGIPTMFGYSGLKAVKYFLDGNS
                     LNMEQVWILLVASMTAYLVSLVVIRFLTDFVKKHDFTVFGYYRIILGAILLVYAFITF
                     LF"
BASE COUNT         4865 a         3754 c         3204 g         4729 t
ORIGIN      
        1 ccaaaggaac ccttttcaca attaacctaa acgctgctta gcatctgctg cacgttggaa
       61 agcttgtccg acatagttga tacagagcat catcacaagg atgaagatgg ctgctggcaa
      121 ccaaatccat gtcttgtttt ccaaaatatc tgcattgcgg gcattggcaa tcaaggtacc
      181 caaacttggt actgtcgatg gcagaccgaa accaagataa gtcaacgaag tctcgatacc
      241 gatatttccc gcaaagttca aggtcaagtt aacgataatc aatgaactta agtttggtaa
      301 aatttcacgg aacataatca taaagtcgct tgttcccaat gtttttgatg catttacata
      361 gtccaaactt gcctctgaca aagtccttgt acggaataga cgagccttgg ccgtccaata
      421 aaaggcactc atgatgagaa tgaaatgata gatcgtatag ttcttgataa ctgtcacgaa
      481 aacaataatc aacattgtca ccggcaaaat catgatgaaa tcgacaatac gcatcaagaa
      541 tccgtccaaa cgaccgccat aataaccaga aatcagaccg acagaaatcc cgacaatact
      601 tgtaataatc gtaatggtaa aaccgataat gattgagtta cgagcgccga taatcagctg
      661 accaaagata tctttaccac cttcatctgt ccccaagata tagccgtcaa cacctggttc
      721 caagtaacgt cccaaaaggt tgactttcat aacttgttct tgattcaaca gaagtgcacc
      781 gataaagact gctaagagaa ccgtcactaa gataattaat gaggtcaatg caagtcgatc
      841 tttcataaac tcccgcgcga tgacacggaa gccacctgga ggagttgaag cagattgtac
      901 ttgtttttta ttttctttac tcaccttact cctccttcta tttcacacgg atacgtggat
      961 ccacgatact catgataata tctgaaatca atgtccccaa caaagtaccc ataccaaata
     1021 ccaacaataa agctaagata acactatagt cacgactaga aatagagtta aagaataact
     1081 gaccgatacc tgggtaagtg aaaatatttt caataaagat agaaccacca attaatcctg
     1141 tcaattcata gcccaagaaa gatgcaattg gcaaaataga attccggaag atatgacggt
     1201 tataaacaac tctctcagga acacctttgg cacgagctgt acgaacatag tcctgactct
     1261 tagcatcaat aatccccgta cggagatatt gaatagtaac tgttgttgac aaaatagcca
     1321 ttgtaatcgc tggcagaatc atatggtgta aacgactgag agtcgctgca aatccttcca
     1381 caccaccact aatcgaaccg cgcgtcggga accagcccaa actaaagcca aacatccaga
     1441 gcatgaggat ggcaaaaacg aaagttggag ttgaaaatgt caagaagtta tacacgccaa
     1501 taattttatc tgcaagtgag ttttgataac gacctgcaat catccccaat ggaagtgcaa
     1561 tcaaataagt caataccatc gtcaagaggg ataatccaat tgtattattc aagcgctgca
     1621 tgatgacatc aacaactggt tgtttaaaaa taacactctg accaaaatct ccttgcaaaa
     1681 gattgcctac ccagcggaaa tactgaatag ggattgggtc atagtaacct gccgcaatcc
     1741 gtttttgttc aatgattgct ggatcaatat tggggtcaat caatcctgta aagggatccc
     1801 ccggcatcat cttcgcaaca aagaacgcaa tgacactcaa aataataatc tgcggaatca
     1861 tcatgagtaa acgacgtaat actgttttcc acatcttagt tctgacctcc ttctggaagg
     1921 gctacttgat gagtttctga gaaggacctc aaatcgtaca cacgaccgtt ttcatcatag
     1981 tattgacctt gcatttcttg atattctttt tcagcagcca aacgcttttc tttattcttc
     2041 aatcgattga gtgggtcaat cgttggaata gctgacaaca aacgtttagt ataaatgtgt
     2101 tgtggattat tataaatatc attcttattt cctgtttcta cgaaacgtcc gcgatacatg
     2161 atgaacaatt catcacacat gtgttgcaca acaccgaggt catgggaaat aaagagatag
     2221 ctgagcttaa actcatcttg gatacgtttc atataattca agacctgtgc ctgcacagac
     2281 aaatccaagg cagaaaccgg ctcatctgca ataatcaaac gaggattact agccaaagca
     2341 cgagcaacac cgatacgttg gcgttgacca cctgagaatt cgtgaggata cttaatcaga
     2401 gcttcttcat ttagaccgat tgtatccaag agttgaagaa cttttttctt ttcttcatct
     2461 ggagataaac ggtcaaagtt acgaattggt tctgcaatga tgtccaaaat acgttttttc
     2521 gggttaaaac ttgaaagaga atcttggaaa atcatttgaa catcacgatt aaagtttcct
     2581 tttttaccac gtgatttgtt ggttacattt tgtccttcat agataatctg accagaagtg
     2641 gcacgctcta aaccgataat tgccttacca atggttgatt tcccagaacc tgactcacct
     2701 accaaaccat aggtttttcc ttcttcaaac tcaatattaa caccatccac agcatagaca
     2761 tggtccgtca cacggttaaa gaagccacta cgaattggat aatggacttt taaatctttt
     2821 acttcaataa atcccactat taggcctccc cttcaaaata gaatgtctca tggcaggtac
     2881 aacgaacaaa gtgatttgga cctacctcgt gcatgctcgg attctcttca tgagcctctg
     2941 ctccaatcca cggaatacgc ggagcaaaac ggcaaccttt acgaaccatt tttgtaatcg
     3001 gcggcacaat accttcaata acatgaagat cgcctccttc gctacctgat tgaggatttg
     3061 acttcaagag cgaacgtgta tatggatgtt ttggattggt aaagagttct tctactggcg
     3121 ctacttcgac aaactgacca ccgtacatga cagccacacg atcagcagtt tctgcaacta
     3181 ctcccaagtc atgggtaatc aaaataatac cagaacctgt ctccgcttga atatcattta
     3241 agagatccaa gatttgtgct tgaattgtaa catccaaggc tgttgtaggt tcgtccgcaa
     3301 taataattgg cggtttacaa gacaaggcca tcgcaataat gatacgctga cgcataccac
     3361 ctgaaagctc atgtggatac tgtttaaatg tccgcttagg atttggaata cctacctgag
     3421 ccaagagttc caaaactcgc tctgtacgag cattggcatc caaatcagtg tgatagaaca
     3481 gcgcctcatc aatctgtgca ccaatcgtca tcaatggatt tagagaagcc aagggatctt
     3541 ggaaaatcat accaatatca ttaccgcgta ctgtattgta cttagcttca ttcatgccaa
     3601 tcaattccat atcattgtac tggatgcttc ctgtgatttt ggtattcaaa gggttatgca
     3661 agcccatgat ggtcgttgcc aaagttgatt taccacagcc agattcccct acaattgcca
     3721 aaatttcatt tttttgcaat gagatgtcaa caccgtcaac agcgtcaaaa taatcatctc
     3781 caatacggaa accgacgtgt aaatctttaa tgtctaaaag cggtttttcg gtagccacgc
     3841 tttttcctcc tttatgtgcc agctaatcaa gctcaatcaa ttcaatagcc ttacgattat
     3901 caagttcata taacgtttga ttaactgact ttatataact actctagaat aacatatttc
     3961 tatcaaatta tcgtttgata gcgattaccc agaagtaaaa aatgtaccta tatccaatca
     4021 taaatcaaga ccatccctat tactgtctca attaggaatt gagtaggtta atgttcttca
     4081 tcttgagttt ttgttagtag ggactcattt ttgtgtactg agtctactat accacaatta
     4141 tctgaaaatt ccaatagcaa tctgaaaaaa gccaatcaaa ttggcttttt cagattctat
     4201 tgtcttctaa caaattattc tgccactaca cctttatcag cagtaagttc gatattctca
     4261 agagcgatac ctttacctga agctgaaccg tagttagtgt catagttttt cacacgtttg
     4321 ttaagtgcta caattgactc agattcaaat gttggaatag caaatgcttg ctcgtgagca
     4381 tatttttgcc aagccttata gttctcaacg tttttcttat catcaaacga ctctgctgag
     4441 ctgatagctt ccaataactt ggtgttttca tcggacacga aacgtgacat attgaaggct
     4501 gcgacagacc cccatagacc acttggattt ggatcgtaac ctgttgacca tccaccagca
     4561 tacatatcaa tagcaggatc attagcttga actgagttgt agaacgactt accttcaact
     4621 gtacgtcctg tgtaaagttc gacattcaag ccaacttctt tccaccaagc aatatattgt
     4681 tgtacaagcg cttcgttagc ttcagtacgt ttacgagcag caaaggtaat tttgaacgcc
     4741 ttaccgtctt taccttcacg gataccatcg ccatctacat ctttgtagcc agcttcatcc
     4801 aaaagctttt tcgctttttc aggattgtat gaatagcctt ccaactctga atcatgaata
     4861 tccccgaaga atgagataat caatgatttt gcagggtggt aaagaccgtt gtacaatgat
     4921 ttgccagctg tcttggtatc aattgcatag gcaattgctt gacgaagttt cacatcattc
     4981 atcttagcct tttcatccat aacgttctta ttagctgctt cgtcatattt accaagatta
     5041 aacgagatat actcataaga cgactctaac tgaccaacga tattcaagtt agaaacatcc
     5101 ttatatgaat ctaactgatc aaccggcatt tcagcaatat cataattccc cgctttcatt
     5161 tcagaaacaa tagtatcagg agatacgatg tcaatcttca atgagcttgt ttttggtttc
     5221 gtacccttga aaaagtattc gtttggtacg taagtgatag actcaccatt aacaatttct
     5281 ttaatcttcc acggtcccat accaacaagc ttcgcagtac gtgagtattc gctcttttcc
     5341 cagtctgcaa cagggatgtc tttatagatg tgttctggtt ggatataggc tgggacatca
     5401 ccacctgcat acatcattga aggtgacatt tcttttacag tcaattctac tgtatagtca
     5461 tcaactttct taataccaga gatgtctgat gcttttccat caacaaattc ttgcatgcct
     5521 tcaatgttca agaatttatc atcaaaacga atacctgtat agtctttgct tgccattgac
     5581 ttaattgtaa agatgtagtc gttgattgtg aatggctcac catctgacca cttgtaatcc
     5641 ttgcctgtca agctaattgt gattttttta cctttcacat caaattcagc cttggcaaga
     5701 cctgagtcgt ccaacttacg ttctccgtcg taaccgaaca ttgaaatatc aacaaaacca
     5761 ccaaatgttg agtcagtcgt attttcagtc aactcatcaa tcaagagacc tgatgaagtt
     5821 gttggtgata cccatgcgta tttcaactga gcgtcagaca ctgctgttcc gtcttgtttt
     5881 acttcagctg ggaatgacaa ttcctgtttt gctgcacctg attgttttga accacatgct
     5941 gcaagaacag atgctgaaag gagagtcaca cctgcaagtg caaaaagttt cgttgtcttt
     6001 ttcataaaac acctctgttt tctttttgtt tgacacttat tactatgtct tataaaaatc
     6061 attatatcat aaatttttct taaataaagt ctttttgtca gaaaattttg taaaaacata
     6121 ttttttcatc attttctcat tttttacatg ttttttccaa atttttctca catttcttgt
     6181 gatataatag aataaaataa cagccaaagg aatgatcatg cgtaaatttt tactaacact
     6241 cactacagtt attgctcttt ttttcacttc cacacctatt ttggcagaag atttttctgt
     6301 tgcagccaag catgctatcg cagtcgaagt aagtagcggc aaaatcttgt atgagcaaga
     6361 tgccgaaacc aaagcaagca tcgcttctat cagtaagaca ttgagcattt acctggtcta
     6421 cgaagcactg gcaaagggtg agatcacttt agatactatg gtagatattt ctgattatcc
     6481 ctatagcctc acttctaata cagaattaag taatgtaaac ctggatgcac ggtcctattc
     6541 agtaagagac ctattaaacg cttctttaat cacatcatcc aatagtgcta ttattgctct
     6601 ggcagaaaaa atagccggta gcgaagctgc attcgtggat cgaatgaagg caaaagtcca
     6661 agaatgggga attaccgata caaaaattgt caatgtatcc gggctagata ataccgactt
     6721 gggtgaaaat atttatccag gttccagtcc tgaagatgta aatatgtttt cggccctaga
     6781 tgttgctatc atcgcaagac gattaatact cgactatcct caggtactcg acattacctc
     6841 gctaaacgcc tacgattttg gcggctacac ttactatagt accaaccaaa tgctgagcga
     6901 cggcactcac gctcgtggag gggtggacgg cttaaaaaca gggacctcaa actcggctgg
     6961 ttcaagtttt cttgcaacaa ctcaacaagc tggtatgcgc attatcacag tagtattgaa
     7021 tgccaatgat gggctgacct ctccagaaaa tcgctttgtc gcgacaaatg atttgatgaa
     7081 ctatgtttat tctaactttt cgcttgttac actagtaaaa aaaggtgaag cctttgaaga
     7141 tagtaagata aaggttttca atggcgaaaa agaaacaagt cctgttgttg cagcagcaga
     7201 tttaaccgtt gttcaacgaa aacaaactga taatgtcccc accgcaactt ttacaacgga
     7261 tataaaagaa attgatgctc ccctcaaagc agggaccttg gtcagcaaac tacaactgaa
     7321 agatcaagat ctcattggaa aaggttatat ttccgagcaa gctagtgtcg atatgattat
     7381 ccctagcgat atgaaagaag catcctggcc attctcttgg tggaatcaat ttgtccgcta
     7441 tgtcaatgaa aaattataaa aaaatagtat gtcgaacgat tttcgacata ctattttttt
     7501 atcgtagagt gacaaccgct actccttatc tccagcctcg gatggtgaga gttcctgcag
     7561 aactctttat tacatatcac ttccttacag gaagcgatgt cagtagggta atctagtctc
     7621 ccagactatg ttatttataa agaagagcga ctgccgtcgc tcctcatctc ctacttcagt
     7681 ggggagtttg acgggcttga acagctttaa agctgttcaa gttcgcggca aactatccca
     7741 ctgaaccctc catctcatag gcaatcagtc tgttgagttc gactgcgtat tccattggga
     7801 gttctttggt gaatggttcg acaaagccca tgacaatcat ctctgtggct tcggattcgg
     7861 ataggccacg gctcatgagg tagtagagct gttcttctga gatttttgag actttggctt
     7921 cgtgttcgag ggccacttgt gagttgtgga tttcattaaa tggaatggtg tctgacttgg
     7981 aaatatcatc cataataata gtatcacact cgatgtggct aatcgacttg gctgagtttt
     8041 tagcaaaggt cacttgacca cggtagttga cttctccacc gccacgggca atggatttgg
     8101 acacgataga agatgaggta cgtggggcat tgtgaatcat cttagcaccg gtatcctgga
     8161 cttgaccttt attggcaaag gcaatggaca acatggttcc acgggctcct tcaccttcca
     8221 agtaaaccgc agggtatttc atggtcgtct tagcaccaag gtttccgtca atccactcaa
     8281 ctgtggcatt tttctcggca cgggcacgtt tggttaccaa gttatagacg ttgtcagacc
     8341 agttttgaat ggtggtataa cgcatatagg caccctcgtg ggcaatgatt tctacaatcg
     8401 ctgcatggag acttgctgtt gagtaggtcg gagctgtaca gccttctacg tagtgaacgc
     8461 ttgctccttc atctacgatg atgagggtgc gttcaaactg ccctgttcct tcattgttga
     8521 tacggaagta ggtttgaagc ggaatatcca gcttgacacc ttttggtacg tagataaagg
     8581 tgccccctga ccaaaccgct gagttgaggg cagccagttt gttatctgac ggcggaacga
     8641 gttttccaaa gtattttttg aaaagctctg ggtgttcttt aagagccgtg tcggtatccg
     8701 taaagataat gccgtgcttg tcgtactctt ctttcatatt gtggtagacc acttctgatt
     8761 cgtactgggc agaagcaccc gccaggtaag cacgctcagc ttctgggata ccgatgcgtt
     8821 caaaggtttc cttgattttg tctggtacat catcccagct gcgtgctggc ttatctgacg
     8881 gtttttgata gtaaacaatg tcatcaaaat ccagttcaga cagatcagca ccccaggtct
     8941 gcatcggcat tttcttgaag gtttcgtagg atttcaaacg gaattccaac atccattcag
     9001 gctcgccttt gatacgggac atttcacgaa tgacttcttc gttcaaaccc ttaccagttg
     9061 aagccactag ttccacgttt tcatcatgga aaccgaattt gtattcccca agatcaatcg
     9121 gggttggttc aattctttct tctgacataa tataagatac taagcccctt aagaaagcaa
     9181 atgaaaaaca ggaaatcgac ttcgtgcgca aacacacgcg gagatttatc ttttttcaaa
     9241 agcttttagg gcgagttcaa tttcatttcc aatcagaaac tgaacaatcc tttctgtatg
     9301 attttcattt ttgtttattc gatgatgctc agtaagcatc ttcacttcgt tcgattgctt
     9361 tcttcaagac actaaacttg gttggaacta aagttcctaa ccaagtaacg caaggttcta
     9421 atcaaccttg ctaagtggct tactaattgg ctgattttgg taatatcgac caaaatcgct
     9481 catgtcgcag ttacccttca ttcagcacta tcacttcgtt ccagtgcttt cttcagggca
     9541 ttccacggta atgtggcaca tttgatgcgt tgggggaatt tggcaacacc tgcgaggagg
     9601 gaggcgtctc ccagtccctt ctggcgtggg tcttcttgac cttggaccat ttgtgagaag
     9661 atttctgcta gttcttgaat ctgactgatt tctttgccaa gcactgcttc agtcatcata
     9721 gaagctgatg cggttgaaat ggtgcagcca acgccgtcaa aagcaatatc ggtaatcaca
     9781 tctttttcaa tcttgactga tagttcaatc acatcaccgc aggttggatt gtggagttct
     9841 aacttttcca ctccgtccaa acttccgcgg tgacgaggca acttggagtg ttcagacaca
     9901 actgccatat aaagagagtc taatctagat agggccattg aaaaactcct ttgtcttgat
     9961 gattgcctcg accaacttat cacagtcagc cttggtattg taaatgtaaa aacttgctcg
    10021 tacagtagca ggcacctgca aatatctgag aagcggttgg gcacaatggt ggcctgcccg
    10081 cacagcaaca ccttcatagt ccagagcagt tgccacatcg tgtggatgca agtcgtccaa
    10141 gttaaaggca ataactcccg tccgcttgga caggtcctga ctgccataaa tggtcaagcc
    10201 tggaatagcc tgcaattttg gaaagacata gtcaaccagt tctgcctcgt gggcctggat
    10261 agcgtccata ccaatttcgg tcaggtaatc aacggctgct cccagaccaa tcgcacctgc
    10321 aatattgggc gttcctgcct caaacttcca aggcaattcc ttccaagtag ctgactgctc
    10381 atagacaaag tcaatcattt caccgccgaa ttcaactggc gacatgagat tgagcaattc
    10441 ttccttgccg tagagaacgc cgattcctgt cggtcccaac attttatgac ctgaaaaggc
    10501 aaagaaatcc acgtctaatt cttgcacgtt gacagccatg tggggaactg actgggctcc
    10561 gtccaccacc aagtaggcac cgacttgatg caccagctct gctatttccc caataggaac
    10621 cacgccacct aggacattgg atacgtgggc cagggagacg aacttggtct tttcggagag
    10681 tatggacttg aggctatcca catccaactc gccgtctttt agtggaacat agcggagctt
    10741 ggcacctatt tgctgacagg cttgctgcca agggatgata ttggagtggt gctcttggac
    10801 cgagattatc acttcctggt ctggctggag aatttccttt gcaaactgag ctacccagtt
    10861 gagactggtg gtcgttcctc tggtaaagag aatttccttg ctagacttgg cctggataaa
    10921 atcagccacc ttctgccgag ccgcctcgta gcgagccgtt gcccgttctg acagagtatg
    10981 aactccacgg tggacattgg cattgtctgt ctggtagtaa tcttccagta catccagtac
    11041 ctgttgcggt ttttgggtgg tcgccgcatt gtccaaatag accagcggct catcgttaac
    11101 aacttggtct aggattgaaa aatccttgcg aatgctttca aaatcaatca tcttatctct
    11161 ttgttagttt ttcttcaatc acggcaatca attggtcacg aacttccttg acaggaattt
    11221 cggtaatgac tgcgccaagg aaaccgcgaa caaccaagcg ttcagcagtt gcttggtcca
    11281 gaccacgact catgaggtag tacatgtctt ctggatccac ctgaccgatt gaggctgcgt
    11341 gtccagctgt gacctcattc tcatcgataa ggagaattgg gttagcatct gagcgagcct
    11401 tgtcagacag catgaggaca cggctttcct gctgggcatc cgcaccttta gcaccacgga
    11461 caatgtgtcc gatgccattg aaggtcaagg ttccgctttc taagataacc ccatgttgca
    11521 ggatgtgacc gaccgaatta tgtccatagt tggtcacgcg cgtatccaca ccttggacct
    11581 gacgacctga tgacagtccg acaactttga ggttggcatg gctaccgttt ccttttaatt
    11641 cactgtccaa atcggcaacc acatttcctt cgttgagcag accaatcgcc cagtcaatcg
    11701 ttgcatcgtt tccgagatag ccgcgacggt tgaggtaggt atccaaatgc ttgccaagac
    11761 ggtcaatagc cgcaaacttg atttggctac cagcaagggc gattacttcc acgacaatat
    11821 tggctgaagt cgttgctgcg ccgtcgccca aactttcaaa gcgttctaga taattaagct
    11881 tggcattttt tccagcgatg atgaggacac gtttgttaaa ggcaactgga ctggtgctgt
    11941 cttggtagaa aagggcctca actggctggt cgatttcaac attatctggc acgtaaagga
    12001 cagctgttgc gttaaaataa gctgtgttat aggcggctaa tttgtgttca tcataagcta
    12061 cagctactcc aagatatttc tcgataacat ctggaataag atccatagct gaggcaaagt
    12121 ccgtaaagac cacacctttt tctacaaggt caaccggcaa ctgttctaga atcgtttggc
    12181 tgccaacttg aaccaacata ggattgtcac cgatagctgt aaaatctgga actgccccga
    12241 tttcatcatt tgtagctagg cttccgtcac ctagattcca acggtgaaac ttgacccgct
    12301 cgataaccgg caagtccagc tctgctattt tgtcaaaagc cttaagacgc aggtctgtca
    12361 accaagttgg ttctgcctgc aaggctgaaa attcttgaat cttttctttg gtcatagctt
    12421 cctccaaaga cttaaatgtc atcttctttg tagtcgatgc caagctctgc tgcgacttgg
    12481 acataacctt ctttttccaa gcgttgtgcc aattctggtc cgcctgaaag cacgacacgg
    12541 ccttccatca tgacatggac aacatcagga gtgatgtagt tgagcaagcg ttggtagtga
    12601 gtaataatca tggcaccaaa gccttcacca cgcatagcat tgatcccctt tgacacgact
    12661 ttaagggcat caatatccaa tcctgagtca atctcatcaa gaagggcaaa agtcggttcc
    12721 aacatgagta attgcagaat ctcattgcgt tttttctcac cacctgagaa accttcgttg
    12781 aggtaacgct ctgccatttc ctctttcatg ttaaggagct ccatcttttc atccaactta
    12841 gtaatgaaat cacgaacaga aatcttgtct tcgtcttcct taccagcgtt cattgcggca
    12901 cgaagaaatt ccgcattagt aatccctgga atttctgacg ggtattgcat agccaagaaa
    12961 agacccatac gggcacgttc atcgacttct aattctaaaa tattgacacc gtcaaacaga
    13021 atctcacctt gggtaacttc gtagcttgga ttgcccatga tggctgctga aagggtggat
    13081 ttccctgttc cgttcggtcc catgatggct gcaatttcac ctgttttaag ggtcaggttg
    13141 acacctttta ggatttcctt accttcgatt tcaacatgaa ggtctttgat ttctaatact
    13201 gacatgctgt gctccttttc atttttgtcc tcttcaaaaa ccacattaac tgaccaagct
    13261 aactagatac accgataaga ctcaaccttg gcagtgggga aagaactcga ccatccatag
    13321 aagagttcgt tttcccaccc ccgcacagtt gattaggtca gatttggagt gtaaaacacg
    13381 aacaaatctg ccaatcaacc actgcgctga gatgttgaca ctaacgttga gaagcggtac
    13441 ggggcttgag cccagcctca ctccactgga ctgttaaacc caatcaacca ctactgctct
    13501 gagatgttga cgctaacttt aagaagcggt gcggggcttg aacccagcct cttttcttgt
    13561 taatgatgat tttcagagag tgttaatcta taaaatagtc ttttctgaca attttaatta
    13621 accatatcca ctatattata tcaaaaaaag agcctgacgg ctcttatctt gtttagaaag
    13681 attcttattt tctttttttc tcttcacagc actttttata ctttgcgatt ttttcttgac
    13741 ggtaactact attaccaata aaccgaagaa gattgagcaa tggagttcga ttaataccaa
    13801 agatgccaat caattcacag agaatctcaa ttcccagtcc catggtcacc aataggagaa
    13861 taccccctat tcgactcgaa acattgagca aaagagaggt cagggagaat aagaaagaga
    13921 tggtgtaaat caccaatact gtgccacgat gagtaagtcc cagagagagc aagcgatgat
    13981 gcagatgcat cttatctgct tcatagattt tttgcccaga caattttcga cgaatgatgg
    14041 ctactacagt atccgtaata ggcactccaa gaataatcat tggagtaacc actgcaacag
    14101 ctgtcgaatt tttcagtcct tgaagggaca agactccaat cataaacccg atgaaaaggg
    14161 ctcctgtatc gccaagataa atgatagcgg gattatagtt ataaggtaaa aatccaacaa
    14221 tcgctgcgac caaaacaaag atggttaagg tcaaaaaaat attgctatca tgcaaaaaga
    14281 aataagacac aattcccatc gttgtcaggg aaataatcga tactcccgaa actagaccat
    14341 ccagaccatc aatcaagttg actgcattcg taatagaaac aatccaaagg accgttaata
    14401 gaaaagagag ccagacagga aattgaagga aaggtcctcc aaaaggaatc ttaaagctat
    14461 caaaacgaaa ttccgtaaaa atccaaatga tcgtcgctgc agctagaata ccaaacattt
    14521 taggagcagg ctttagctcc ttgacatcat caatgaatcc cgtaagaacg ataatgctgg
    14581 atgctaggac caggggccag atataatgca agtaagtgcc atggtaattg acacgggtaa
    14641 caattcctgg taaaaaccat aggacagaaa tggtaaatgc cacaaagata gccagacctc
    14701 ccgctgaagg catgggcacc ttatttattc gccgtgcatt gggattatcg actgctccaa
    14761 ccctcaggga cataaaacga accaaggggg ttgcaatagc tgatatgacc atagttgtaa
    14821 taatgaccat tatgtatttt agagcaaaag gaatcatact gtcggaacct tcctaagctc
    14881 ctcgattgcc gcaagcggaa gcatcaaagt tccgtgttct tgtaaagcag gtctggtcaa
    14941 cttagtatca ttggtaaatt ccaacattct tgagagaatg tagcctggat actgcttgga
    15001 acgattttct acattgataa gaactgtcaa gtagtagtga ccatccattt tatataattc
    15061 cgattcttct actggataat caaccgtacc aacaaaagta aacagatcct ctatcgttgg
    15121 aaattccaaa atatagtaaa tatagcgctc ctgctcctct tcatctgctt cttcctcttg
    15181 ctctacctct tccaaatgac gaactgctgt tgcatccggt gcacttttct cacgaacggt
    15241 ttgctccaaa ctcttgaaaa actcatctgg agtcatactt gcgacatcac caagttccga
    15301 caaatctgta aattcatcaa aattcaagtt ctgatccaaa tcagatttgg taacaaagat
    15361 gtccacccga tctggcttcg gagtcacacg aaaactcaac ataccgcttt ctcggaaagt
    15421 cattggtaaa tccaattcat ccaaaacagt atagaagaac tcctctgtct tttcttgcgg
    15481 aatgagaaag tccgaaagtt ccattcctct ctcttccaaa tcatctaatt ttatagtgat
    15541 tttcaaggtt gaatcactaa tttgctttac tttcataact acctcattca ttgattcatc
    15601 aatttcttaa aattgattga tcattttcaa tactcataca tatcttccac cattatacta
    15661 aaaccaagct cgaaagacaa aagaaaagca ggtctccctg ctcttaaaat aaaaatgtaa
    15721 taaaggcata aaccaataaa atcgccccta aaatgatgcg gtagtaacca aaaaccgtga
    15781 aatcatgttt tttaacaaag tctgtcaaga aacgaataac aactaaggat actaagtaag
    15841 ccgtcatact agcgactagc aagatccata cctgctccat attcaggcta ttgccatcta
    15901 agaaatattt aacagccttc aagccactat aaccaaacat ggttggaatc ccaaggaaga
    15961 aggtaaaatc tgccgccaca gatcggctgg tccccaaaat aatcgctccc agaatggtcg
    16021 cacctgagcg actagtcccc ggaacgatgc ttaagacctg aaaacaccca atcaacaagg
    16081 cagttttata agacattttc gctaagtccg tcacctgtgg ctctacacca ctgttacggt
    16141 tctcaatcca aataaaggca ataccgtaga caatcaaagc aatcgcaatc ggtaccataa
    16201 aattgaagtg cgcttcaaac caattatcca acgggactgc aattaggatg gacggaatac
    16261 aggcaatgac aaccttggcc cataattgcc aagtcaattg aatatcacgc ttggtttttc
    16321 caggctgaaa tggattcaat tttttaaagt aaatagtcat aactgctaga attgctccta
    16381 gctgaatgac aatattaaac atttctagga agttcttgct ttggtttaat ttcacgaact
    16441 cttgaaccaa aattaaatgc cccgttgaag aaactggcaa ccattccgtc accccttcaa
    16501 tgattcctaa aaatatagcc ttcaacaatt ccaatagcat aaaaatctcc tt
//
LOCUS       DAZQCJ010000037        16390 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000037 DAZQCJ010000000
VERSION     DAZQCJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16390
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.037"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            180..434
                     /locus_tag="ACHU66_001102"
     CDS             180..434
                     /locus_tag="ACHU66_001102"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389394.1"
                     /translation="MNKRTKKKYKPFKELWDCMEWVMFRLNRHVTRLDSLENRLENMR
                     VIDSANIQTINRKFEEYEKRVETLEREVKQLKKPWYKRRK"
     gene            434..664
                     /locus_tag="ACHU66_001103"
     CDS             434..664
                     /locus_tag="ACHU66_001103"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389395.1"
                     /translation="MAEVVTMDELAKNKERGVGCRGILKAELETVTNIESIVIVSLNK
                     NNQINVSYSRNSSLEALGMLDIGKAILLDEMQ"
     gene            748..2163
                     /locus_tag="ACHU66_001104"
     CDS             748..2163
                     /locus_tag="ACHU66_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR3389396.1"
                     /translation="MVTKTKTRLGNQRPTQSVTLHYAESLAHEAIELYQKTGRSCYPW
                     QVNLLEHIMGIDTEGLWVHQKYGYAIPRRNGKTEDVYMLELWALHHGLKILHTAHRIS
                     TSHSSFEALKKLLEDMGYVDDVDFVSNKAKGQERIEFKSTGSVIQYRTRTANGGLGEG
                     FDLLIIDEAQEYTAEQESALKYTVTDSDNPMTVMCGTPPTMVSTGTVFTNYRTKVLAG
                     KSEYSGWSEWSVEDIKDVNDTDSWYLTNPSLGYHLTERKIKAEIGDDELDHNIQRLGY
                     WPSFNQKSAISENEWKRLEVEAVPELTGKLFVGIKYGQDGANVSMAIAARTDDKKIFT
                     EVIDCQSVRNGMQWIINFLQTADIEKVVVDGANGQTLLANEMKDFKLRKPIMPTVSEI
                     ITANSVWEQAIVQETLRHANEPSLTDVVTNCDKRNIGSNGGFGYKSLYDDRDISLMDS
                     ALLAHWACYMTKPKRKQRISY"
     gene            2244..2708
                     /locus_tag="ACHU66_001105"
     CDS             2244..2708
                     /locus_tag="ACHU66_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFR3389397.1"
                     /translation="MMSFKPIETQEELDRIIGERLGRQKEKYADYDQLKNRVSELEKE
                     NGVLKSAAESTKASTSDLEKQIAELQGQVKTYEGKDLRLRVAVANGLPIELADRLAGD
                     DEEAIKADAERLASFMKPTEPTPPMASTEPNVESGSAEDEALKGMLRKMRGE"
     gene            2712..3602
                     /locus_tag="ACHU66_001106"
     CDS             2712..3602
                     /locus_tag="ACHU66_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFR3389398.1"
                     /translation="MATLRSGDMFPEQTVKDIFSKVKGHSTLAKLSTQEPIPFTGTDT
                     FVFNLEGNAEIVGEGEPSNAGNATMKPKVIKPVLITYQARVSEEFVKCSEEKQLNYLK
                     SFIDGLAKKVAQAIDIASFHGLEPKSMTAASFKETNSFDGLITGNVVEYDANTIDECI
                     DAAVATVTANEGEVNGIALSPAAGAALGKIKVNGVVQYPEYRFGQNPEAFYGMKSDVN
                     KTLATVASGAKKDFAIVGDFENALKWGYAENIPLEIIEFGDPDGAGRDLKRYREVCLR
                     TEVYAGWGILDPESFARVEG"
     gene            3604..3822
                     /locus_tag="ACHU66_001107"
     CDS             3604..3822
                     /locus_tag="ACHU66_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HeH/LEM domain-containing protein"
                     /protein_id="HFR3389399.1"
                     /translation="MEYINKKTKATVETDAKLAGDWVPASEFEKAIENLTVPQLKGKL
                     DELGIEYNKKAQKAELLELLEAAKSEME"
     gene            3826..4221
                     /locus_tag="ACHU66_001108"
     CDS             3826..4221
                     /locus_tag="ACHU66_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015971737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp19/Gp15/Gp42 family protein"
                     /protein_id="HFR3389400.1"
                     /translation="MEPFATIEDLSTLWRGLKPAETKRAEALLKVVSDSLRVEAEKVG
                     KDLDKMVEAKSYFATVVKSVTIDVVARTLMTSTDQEPMTQYSESALGYSVSGSYLVPG
                     GGLFIKDSELKRLGLKKQRFGVIELYDKD"
     gene            4208..4546
                     /locus_tag="ACHU66_001109"
     CDS             4208..4546
                     /locus_tag="ACHU66_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389401.1"
                     /translation="MTRINGRPIVLIDKQVIGKDSFGHPKTADVEIEVENVLIAPATT
                     EDITNQINLTGKKVVYTLAIPKGDMHDWTNKEVRFFGQRWRTVGEPLEGLEHLIPLGW
                     NKKVQVERYG"
     gene            4539..4778
                     /locus_tag="ACHU66_001110"
     CDS             4539..4778
                     /locus_tag="ACHU66_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389402.1"
                     /translation="MARMKFKLNRAGVRDLLKSPEMQAVLTDKANAIRNRAGDGYESD
                     IYVGKTRANAMVYADSIKAKRDNKKHNTLLKAVKS"
     gene            4775..5110
                     /locus_tag="ACHU66_001111"
     CDS             4775..5110
                     /locus_tag="ACHU66_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389403.1"
                     /translation="MIEVITLNFLTEHLSVPVYTEHEEEMPDSFVVFEKTSGGKKNHL
                     NQATLAIQSYGQSLEEAAFLNEEVKQAVEKMVELPSISRVELNSDYNFTDTETKRYRY
                     QAVVDFIYF"
     gene            5126..5713
                     /locus_tag="ACHU66_001112"
     CDS             5126..5713
                     /locus_tag="ACHU66_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR3389404.1"
                     /translation="MADAKLVSSAKPDIAGAISSAPTGTSLPTNATTKLNTAFKNLGY
                     ISEDGLTNEDTRESEELKAWGGDVVDTPQTGKSDKFTYTLIEVLNVDVLKEVYGPENV
                     KGDLESGIAVEVNSKELPTHPLVVDMLLKNGAKKRIVIPNAKVLEVGEITYADSDLAG
                     YETTIQALPDSKGNTHYEYIKGASESTGTSSPSSS"
     gene            5724..6002
                     /locus_tag="ACHU66_001113"
     CDS             5724..6002
                     /locus_tag="ACHU66_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389405.1"
                     /translation="MFEVKTSTGLVLSIDQDRLENYELFEAIAAEESGDSGAMIRIVN
                     LLLGDEAKKLKDHVRTEKGLVPISALGAEIKDIFEQVKDLKNSQSSPE"
     gene            5999..6382
                     /locus_tag="ACHU66_001114"
     CDS             5999..6382
                     /locus_tag="ACHU66_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5361 domain-containing protein"
                     /protein_id="HFR3389406.1"
                     /translation="MIAVDEDALVCDLAETYGIYDYRQLPINRVAVFACGLSESSRIK
                     KVLSGQKEDLDTLLLAGIYDTVRLLFWAKTKDGQAGRNRPNSVAQALVGSKAEREERV
                     FSSDEEFERAMRALEIEIGGEEHGD"
     gene            6372..8954
                     /locus_tag="ACHU66_001115"
     CDS             6372..8954
                     /locus_tag="ACHU66_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR3389407.1"
                     /translation="MATDLGSAYVQIVPSAKGISGSISKLLGGEVDSAGRSAGSSLGA
                     SLVSALSGALAAAGIGKIIGSALGAGADLQQSFGGLDTIYDGAQESAKQFAKEAYKAG
                     ISANTYAEQAVSMGASLKQALGGDSTKAINMANVAIMDMTDNAAKMGTDIGIIQQTYQ
                     SLARGNYAMLDNLKLGFGGTKAEMERLLTTAEGLPSAMGRKFDISNYADVVEAIHLVQ
                     ESMGIAGVAAAEAQNTYSGSLAAMKASWENTLAGLSLGENIAPQLQALATTTSNFLFG
                     NFFPMVGNIFKGLPTMLGTILGDGLGRVFGGEVSGKVMGELNKLNEIILTFYDMTFGS
                     LSEKDNIDMLEKVGFSKETASKIVSLSSQIGSVITSFYDMIFGSLSEKDNIDFMAQMG
                     VDEGTATTIVNFANTIRTGFEGVWSTVQTLFGQVPGFFSSIIGAIGPIITTIMDGISK
                     LDFSGIQTLIESVLPAVQAGFQTFMSIVSPAIDSVVQSFVSMWNAAQPLITILSGALM
                     PVFQILGSFLGGVLKGVLSGVSFAFDALKVAIEFLTPVVDFLVQALNFVQPVLSTIAE
                     WIGVAIGMFGNLGTAGQGLSAFIKSAWTNIQSAIQTAGNIIRTVIDWIKLAFSGAGNA
                     VGVLKNVFSLAWMGIQDAIQVAKGVIDGVISGIKGAFTSFQSVVSSVGGAVNGVITNV
                     ISTIRGIANIDLSGAGSAIMNGFLGGLQSAWEGVKSFVGGIAGWIAENKGPISYDRVL
                     LKPAGLAIMQGLNTNLQVGFKDVMGTVSGMAGAIAKPFENQSLAYDMTSSASVDVRRN
                     LLSSTGDLAGNDGGNSLATRLANIERFLSALVDKELAVYLDGEKLAQNSYMHQGAIMA
                     REGI"
     gene            8954..9649
                     /locus_tag="ACHU66_001116"
     CDS             8954..9649
                     /locus_tag="ACHU66_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR3389408.1"
                     /translation="MNYMIINGLDTSTLADCHVLDFGKAQTSVERSEQVEVFGANGQL
                     HVSEGAYDGYNRTFIITLRHLADAMRLIEVFQSENNTVEFGYLRDSIFYCNLVSSSYV
                     PLGPHRWKVEITVSMHPFRYVKNPADVVLTSSGSVQNPGTVYSEPIIIIEGSGRVTLT
                     IGQQLMELELDTRATIDCRHKRQNIYDKNGAVKNTIRKRGPFFEIAVGRSGIATSGTV
                     SKITIKGNWRYKV"
     gene            9646..14952
                     /locus_tag="ACHU66_001117"
     CDS             9646..14952
                     /locus_tag="ACHU66_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFR3389409.1"
                     /translation="MIYLKDGNIPLNLAYDDDIVQEANSTYQLSFKFPLTDGKWNLLR
                     REVFLLADDLHGEQEFFIFEVKKAKGHVQVYAKQVATLLNYYSINSISVDRVPGQTVM
                     TALAGSVKRPCPFTFFSDILDRHTFNESNVSVMAALAKDKHSIVGQWGGDLVRDKYQV
                     KLLKNGGIENESLFMYKKNLSSYEESENINNLKTRLHLKKTIKGQSEGEADRVIAVTV
                     DSPLIGQYRQIYEADIEVNDQDVTDVASLTAYGKRYFSSTLCDLVENSINLDVKGKSD
                     VSVKMFDMVSVFHERFDVDLRLKISSYHFGPMSKRLKSIGFGKVSQSFGSTVASMVAG
                     SVDKATGRLSASFEQKLQKEIDNANRHFDAEFDKRVESINDGIEQAQAEAERYADAIK
                     QEIDTEIAQVNQSMQAQSEEHDRQVADILSKTQSVESLANQAKSDAASALARANQVKT
                     EAIADARAQVATVSQALNTAKSELQTAIASADQKARDSQASATALRNDLNLQASKILE
                     QARAQTALTNRVTTVETLADGTRSTVAELSKTVNKATGDITSVTARTKTVEDTLSQTR
                     TQYEALTQTVNTQTGQIESINRKTADLQSGIDGVTERFENLRVGGTNLFKNSDFSQGE
                     KNWHKLPEIHNEATGKYVRLPARLWTMAQFVEVEQGEDYVISLYAKKISESSSSPRLN
                     IKFDSLHNEDTDYVEITKTDWKRFIFKFRAKKSGKELVYFLNRNGIEVDIKNIKMEKG
                     LLATDYSPSYEDYRSEIATYKRTAEESSAELSRQIQLADGKAVEAKTYAQQTAEGFKT
                     RLESLESYKDAEGTRANQYLTASRTETAKQLSAERAAIATNYVAKSTYDENVRGTTLK
                     LNEIKTTADTAKQNLATYQNTVDRKLEELTTSTQTLDGKINTASAKVDTVAGQIRTEI
                     SEVEGKIPITAGTRNLLKGTKELTDIYWTSNVTSDYYQGFRIARTAPSAATYIDTYRA
                     STTIVPDATEYIISFYAKSSINGTPINNHFYSPNTTTRSESNTGYIGKGTDGLAIINL
                     TTTWQRYWIKWTQTPSNTKKNVIIGRNFSANNATVEIAGVALYEGSLNKDWSPAPEDF
                     ANELSSVKTTITQTASGVEQLSTSLTTTDSKVTTAEAKIRQLISDVSSKVSQTDYNTL
                     TGRVDSAETAITQNATEISKRLTKTQVDKAITDKGYQTKSDVDSNITGRGYITNSALQ
                     PYALATTVQNLVRETADSFSRTISETKALIPTSVGGRNLIRGSSEMIIGSGRWQDGTF
                     RKSGTGNIKTINISNPPVPNVTKGIEVEIASATECGIAQDTLFLPKGTYTFTVWVKGP
                     TGATGRIDTFNGSGSKSKVFKLTGGWDKVTVTNTSTVDETKNVGYVYLQNSPGYPAKM
                     HLTAPKLEAGTIPTDYTLAIEDLATVTALHDVKDTVSSHTRTIGAVGETGSILDNVSK
                     VTQTAAGLVQEVSGDNGLKTQVSQLAGSYAIKNLTSSGKVLNQLNLNKDGSVKIDGSL
                     VQITGRTYIQDGVITSAKIAGLDAGKVTTGYLASARIKANSIDGSKIAFDEAFFNRLV
                     ANQAYLKKLFAKDAFLTAVQAVSLSASQIIGGLMRATNGAMEIDFNNSAIDMFKDGAI
                     RFHSGSNAIYRQSTDGIHTAFVHFDTTSKGGIYASLGSTSSRDGINSKSSGRFAGIRV
                     ARTSERSDSHVASQDAVELYGDTIFFGHGFEGGGFSMHTTLTGGRTISLNKMHDALIN
                     LCRCWIHLRNEGFNLNSSAFRKSLDTEIHNAQQLYNTIERIT"
     gene            14952..15167
                     /locus_tag="ACHU66_001118"
     CDS             14952..15167
                     /locus_tag="ACHU66_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389410.1"
                     /translation="MNQELINQALRLTNNDLMTKLSEEMTTKNLLAVQLTEAQQTIAN
                     LRAEITELTKQLDEATKPEEIIEQKGE"
     gene            15170..15514
                     /locus_tag="ACHU66_001119"
     CDS             15170..15514
                     /locus_tag="ACHU66_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389411.1"
                     /translation="MTQTTNNTLLNLEETTQPFDLATALRYMKEHGEFIRCKSATQDF
                     YMYRDVQKRPAIVNGRRKFVDVETIWAFNQWGSTTTTINIADMLNEEYWIMKFDEHGN
                     PDWSDPTVGAEV"
     gene            15524..15946
                     /locus_tag="ACHU66_001120"
     CDS             15524..15946
                     /locus_tag="ACHU66_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR3389412.1"
                     /translation="MMLSKTPPHPAGMFEFLRELIATEDGLVLFLLGLIVTMEIVDFL
                     SGTFAAMINPDIEYKSKIGINGLIRKMMGIILLTVLIPMSVLLPEQTGVAFLYTIYVG
                     YLILTFKSLIENYGKAKGDTSIFENVTAAFEKLIGKGK"
     gene            16043..>16390
                     /locus_tag="ACHU66_001121"
     CDS             16043..>16390
                     /locus_tag="ACHU66_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PlySs2 family phage lysin"
                     /protein_id="HFR3389413.1"
                     /translation="MTTVNEALNNVRAQVGSGISVGNGECYALASWYERMISPDATVG
                     LGAGVGWVSGAIGDTISAKNIGSSYNWQANGWTVSTSGPFKAGQIVTLGATPGNPYGH
                     VVIVEAVDGDRLTI"
BASE COUNT         4923 a         3136 c         4036 g         4295 t
ORIGIN      
        1 ctaaaacagg tgtcttgcca gtttttgttg cgacgtctag cgtcaaaaga gttctcgact
       61 taacaagata cctctacttt atcataaaga aaaagtagtg agtactttct cccgtcggag
      121 atttcctcac tactaatctt agtatgtttt tgttgtagta aaacagaaag gaggtcgcta
      181 tgaataagcg taccaagaag aaatacaaac cattcaaaga attatgggat tgtatggaat
      241 gggttatgtt tagattgaat agacatgtta ctcgactgga tagcttggag aatcgtttgg
      301 aaaatatgag agttattgat tccgcaaata ttcagaccat caatcgtaag tttgaagagt
      361 acgaaaaacg ggttgaaaca ttagaacgtg aagtcaaaca gcttaagaaa ccatggtata
      421 aacggaggaa gtgatggcag aagtggtcac aatggatgaa ttagccaaaa acaaagaacg
      481 aggagttggt tgccgtggta ttctcaaggc agagctggag acagttacca acattgaatc
      541 aattgttatc gttagcttga acaaaaataa tcagattaat gtttcttata gtcgcaatag
      601 cagcttagag gcattaggta tgttggatat tggtaaagct attttgttag acgaaatgca
      661 ataggaaccg tgcggaatcc catacggttt tcgatttgcc ctgtcgcatg gcgttaaact
      721 aggcaactac gattgaaagg atatgctatg gttactaaga cgaaaacaag gcttggcaat
      781 cagcgtccaa ctcaatcggt aactttacat tatgctgaaa gtctggccca tgaggctata
      841 gaactttatc agaaaacagg tcgcagctgt tatccttggc aggtcaattt gcttgagcat
      901 atcatgggaa ttgataccga aggcctttgg gtacatcaga aatacggcta tgctattcca
      961 cggcgaaacg gtaagacaga agacgtctat atgcttgagt tgtgggcttt gcaccatgga
     1021 ttgaaaatct tgcacactgc acacagaatc agcacgtctc attcttcttt tgaagcattg
     1081 aaaaaacttc tggaggacat gggatacgtg gatgatgttg actttgtttc taacaaggca
     1141 aaggggcagg aacggattga gtttaaatct actgggtcag tgatacaata ccgaactcgg
     1201 acagccaatg gcggtcttgg tgaaggtttt gatttgctga ttatagatga agctcaggaa
     1261 tacacagccg aacaagaatc agctttgaaa tacacggtaa cggacagtga taaccctatg
     1321 acggttatgt gtggaacacc accaaccatg gtatcaacag gaactgtctt taccaattac
     1381 cgaacaaagg ttttagccgg taaaagcgaa tactcgggtt ggtctgagtg gtcagtcgaa
     1441 gacatcaagg atgtcaacga tactgactcc tggtatttga ccaatccgtc tcttggttat
     1501 cacttaaccg agcgtaaaat caaggctgaa attggagatg acgaacttga ccataatatt
     1561 cagcgtttag gttactggcc aagctttaac caaaaatctg ctatctctga aaatgagtgg
     1621 aagaggcttg aggttgaggc ggtgccagag ctaacaggaa aactattcgt tggtatcaag
     1681 tatggtcaag atggggcaaa tgtgtctatg gctattgcag cacgaacgga cgacaagaaa
     1741 atcttcactg aagtgattga ctgtcaatct gtccgaaatg gcatgcagtg gatcatcaac
     1801 tttttgcaaa ccgcagacat tgagaaggtt gtggttgatg gagcgaacgg gcaaacacta
     1861 ttagcaaacg aaatgaaaga tttcaaactt aggaagccaa tcatgccgac agtcagcgaa
     1921 atcatcacgg ccaactctgt ctgggaacaa gctattgttc aggaaactct aagacatgct
     1981 aacgagcctt ctttgactga tgttgtgacc aattgtgata aacggaatat aggctcgaac
     2041 gggggatttg gttacaagtc cctttatgat gaccgagaca tcagcttgat ggatagtgct
     2101 ttgctagcgc actgggcttg ctacatgacg aagcctaaaa gaaagcaaag aatcagttat
     2161 taaaagtagc atccagaaat tgggtgcttt tttaatgcac aaaaattacc gaacgcacgg
     2221 gaaaatgcgg agaaaggacg ttaattatgt catttaaacc aattgaaaca caggaagaac
     2281 ttgatcgcat tatcggtgaa cgcttagggc gtcaaaaaga aaaatacgct gattatgatc
     2341 agttgaaaaa tcgtgtttca gaacttgaaa aagaaaacgg tgtgttgaaa tctgcagctg
     2401 aatcaactaa agcaagtaca tctgatttag aaaaacaaat tgcagagctg caaggtcaag
     2461 tcaaaactta cgaaggcaaa gacttgcgct tgcgtgtagc agttgccaat ggcttaccga
     2521 ttgaacttgc tgaccgtctt gcaggagatg atgaggaagc catcaaggca gatgccgaac
     2581 gtttggctag ctttatgaag ccgacagaac caacaccgcc aatggcatca acggagccga
     2641 atgtagaaag tggctctgca gaagatgaag ctctgaaagg gatgcttcgt aaaatgagag
     2701 gagaataaca gatggcaaca ttgcgatcag gggatatgtt cccagaacaa acagtaaaag
     2761 atatttttag caaagtaaaa ggacactcta cactagctaa actttcgaca caagagccta
     2821 ttccattcac aggaacagat acatttgttt ttaacttgga aggcaatgct gagattgttg
     2881 gtgaaggcga gccatcaaat gcaggcaacg ctacaatgaa gcctaaagta attaaaccag
     2941 ttttaattac ttatcaggcg cgtgtatcag aagaatttgt taaatgctca gaagaaaaac
     3001 agcttaatta cctcaagtcg ttcattgatg ggcttgccaa gaaagttgct caggcaattg
     3061 atattgcctc attccatgga ttggaaccaa aatcaatgac ggcagcttcg tttaaagaaa
     3121 caaactcatt tgatggcttg attactggaa atgtcgttga atatgatgca aacacaattg
     3181 atgaatgtat tgacgcagcg gtagctacgg ttacagcgaa tgagggcgaa gtcaacggta
     3241 ttgcactttc cccagcggct ggtgcagcac ttggaaaaat caaagtcaat ggtgttgttc
     3301 aatatcctga ataccgtttt ggccaaaacc ctgaagcatt ctacggaatg aaatcagatg
     3361 tcaataaaac attagctaca gttgctagcg gagctaagaa agatttcgcc attgtcggtg
     3421 actttgaaaa tgcacttaaa tggggatatg ctgaaaacat tccgcttgaa attattgaat
     3481 ttggtgatcc agatggcgct ggtcgcgact tgaaacgcta ccgtgaagtt tgcttgcgta
     3541 ctgaagtata tgcaggttgg ggaattctag acccagaatc atttgcacgt gtggagggat
     3601 aacatggaat atatcaacaa aaaaacaaaa gcaactgttg aaacagatgc aaaactagct
     3661 ggagactggg tgcctgcttc cgaatttgaa aaagccattg aaaatctaac tgttccacag
     3721 cttaagggaa aacttgatga actcggcatt gagtacaaca agaaagccca gaaggcagaa
     3781 cttttagaat tgctggaagc tgctaaatca gaaatggagt agtccatgga accattcgct
     3841 acaatcgaag acttgtccac attgtggcgg ggactaaaac cggccgaaac caagagagca
     3901 gaggctcttt taaaagtcgt ctccgactct cttagggttg aggctgagaa agtaggtaag
     3961 gacctcgaca agatggtcga agcaaagtct tatttcgcca cagtggtcaa gtctgtgact
     4021 attgatgttg tcgcacgtac ccttatgaca tcgactgacc aggaacctat gacgcagtat
     4081 tccgagtcag cccttggcta ctctgtctct gggtcttatc tggtccctgg tggcggtctc
     4141 tttatcaaag actcggaact taaacgctta ggtctgaaaa agcaaagatt tggggtgatt
     4201 gaactttatg acaaggatta atggtagacc gattgtcttg attgacaaac aggtcattgg
     4261 caaggattcg tttggtcatc caaagactgc agatgtggag attgaggttg agaacgtctt
     4321 gattgcgcct gcaacaaccg aggatattac caatcaaatc aatcttacag gtaaaaaagt
     4381 tgtctatact ctcgctattc ctaaagggga tatgcacgac tggacgaaca aggaagttcg
     4441 attttttggc caacgatgga gaacggttgg cgagcctttg gaagggcttg agcatttgat
     4501 tccgctcggg tggaataaga aagtgcaggt ggaacggtat ggctagaatg aaatttaagt
     4561 taaaccgtgc tggtgtccgt gacttgttaa agtcgcctga gatgcaggca gttttgactg
     4621 acaaggcaaa tgctatccga aaccgtgcag gcgatgggta tgaatcggat atctatgttg
     4681 gtaagactcg tgctaatgct atggtttatg cggatagtat taaagctaag cgggacaaca
     4741 agaagcacaa taccttgtta aaggcggtaa aatcatgatt gaagttatca cgttgaactt
     4801 tttgaccgaa catctttctg tgcctgtcta tacggagcac gaggaagaga tgccggatag
     4861 ctttgtggtc tttgaaaaga ccagcggtgg gaaaaagaac catctgaacc aagcgacttt
     4921 agcgatacaa tcttatgggc agtctttgga agaggctgcc tttttgaacg aagaagtcaa
     4981 acaagctgtt gagaagatgg tggaattgcc gtctatcagc agggttgagt tgaactcgga
     5041 ctataatttc acagatacgg aaaccaaacg ctatcgctat caagcggtag tggattttat
     5101 ttatttttga aaaggagaaa attgaatggc agatgcaaaa cttgtgtcat cggcaaagcc
     5161 tgatattgct ggggcgattt cctcagctcc aacgggaacg agcttgccga ccaatgcgac
     5221 tactaagcta aacacagctt ttaaaaactt aggctacatt tcagaggatg ggttgaccaa
     5281 cgaggacact cgtgaatcgg aagaactgaa agcctggggc ggtgatgtgg ttgatactcc
     5341 tcaaacagga aaatcagaca aatttaccta cacactcatt gaggtgttaa atgtggatgt
     5401 cttaaaagag gtctatggtc ctgaaaatgt gaaaggcgac cttgaatcag ggattgctgt
     5461 ggaggtcaac tctaaggaat tgcctacgca tccgttggta gtggatatgc tgctgaaaaa
     5521 tggtgcgaag aagcggattg tcatcccaaa tgctaaggtg ctggaagttg gggagattac
     5581 ctacgccgac agtgacttgg ctggttatga aacaactatc caagctctgc cagatagcaa
     5641 aggcaataca cactatgagt acattaaggg agctagcgag tcaacaggaa caagctcgcc
     5701 atcgtcatcg taaggaggtt tgaatgtttg aagtaaaaac tagtacaggt cttgtgctta
     5761 gcattgacca agaccgtttg gaaaactatg agctttttga ggcaatcgct gctgaagaat
     5821 ctggagacag tggtgccatg attcggattg tcaatttatt actcggtgac gaagcgaaga
     5881 aactcaagga ccatgtccgt acagaaaaag ggctggtgcc aatttctgct cttggtgctg
     5941 aaattaagga cattttcgaa caagtcaaag acttaaaaaa ctcgcaatcc tcgccagaat
     6001 gattgcggta gatgaggatg ctcttgtctg tgatttggct gaaacctatg gcatatatga
     6061 ttatcgacag ctacctataa atcgggtagc tgtttttgct tgtggtttaa gtgagtcatc
     6121 acggattaag aaggtcttgt ctggtcagaa ggaagacttg gatactctgc tgcttgcagg
     6181 tatctatgat acggttcgct tactcttttg ggctaaaact aaggacggac aggccggacg
     6241 gaatcgtcca aactctgtcg cccaagcctt ggtaggttcg aaagcggaac gtgaagagag
     6301 ggtcttttca tctgatgagg agtttgaacg tgctatgcgt gcgctagaaa tagagattgg
     6361 aggtgaggag catggcgact gatttgggtt ctgcttatgt gcagatagtt ccgtctgcta
     6421 aaggcattag cgggtcgatt tcaaaattat tgggtggcga agttgatagc gctggtaggt
     6481 cagctgggtc aagtcttggc gcttcactcg tctctgcttt aagtggcgca cttgcggcag
     6541 caggaattgg gaaaattatc ggttcggctc ttggagctgg tgctgatttg caacaatctt
     6601 ttggtggtct ggatactatc tatgatggtg ctcaagagtc tgctaagcag tttgcaaaag
     6661 aggcctacaa ggctggaata tcggcaaaca cctacgcaga gcaagctgta tccatgggtg
     6721 ccagtctgaa acaagctctt ggtggtgatt cgaccaaggc aatcaacatg gcaaacgtgg
     6781 ctatcatgga catgacggac aatgcggcta aaatgggtac cgatatcggt atcatccaac
     6841 agacctatca aagtttagcc cgtggcaact atgccatgct agataatctt aaactcggct
     6901 ttggtggcac aaaggctgaa atggagcgat tgttgacgac tgctgagggc ttgccgtctg
     6961 ctatggggcg caagtttgat atcagcaact atgcggatgt tgtcgaagct atccaccttg
     7021 tgcaagaaag catggggata gctggagttg cagctgccga agctcagaat acttattcag
     7081 ggtccttagc tgctatgaaa gcgagttggg aaaatacact ggcaggcttg tccttgggcg
     7141 agaatatagc tccgcaatta caagccttgg caacgacgac ttctaatttc ttatttggaa
     7201 atttctttcc catggttggt aatatcttca agggtttgcc gacaatgctt gggactattc
     7261 ttggagacgg tttaggtaga gtctttggcg gagaagtgtc tgggaaggtc atgggcgagt
     7321 taaataagct aaacgaaatc atcttgacat tttacgatat gacttttggt tccttgagcg
     7381 agaaagacaa tatcgatatg ctcgaaaagg ttggctttag taaagaaaca gcttcaaaga
     7441 ttgtgtcttt atccagtcaa attgggtcgg tcattacgtc gttttatgac atgatttttg
     7501 ggtcgttgag tgagaaagac aatattgatt ttatggctca aatgggggtc gatgaaggta
     7561 ctgcaacaac gattgtcaat tttgcaaata caattcgaac tggctttgag ggtgtttggt
     7621 ctactgtaca gacattattt ggacaagttc ctggtttctt ctcttcgatt attggagcga
     7681 ttggaccaat cataacgact atcatggatg ggatttcaaa actggacttt tcaggtatcc
     7741 aaacgttgat agagtctgtt ttgccagcag ttcaagcagg tttccagacc ttcatgagca
     7801 ttgtcagtcc tgccattgat tcggtcgttc aatcgtttgt gtctatgtgg aatgcggcac
     7861 agcctttgat aaccattttg agcggtgctt tgatgcctgt ttttcaaatt ctggggtcat
     7921 tccttggagg agtcctcaaa ggtgtcttgt cgggtgtgtc gtttgcattt gatgctttga
     7981 aagttgctat tgagttcttg acacccgtag tcgatttttt ggtacaggct ttgaatttcg
     8041 ttcagcctgt gctgagcacg attgctgaat ggattggtgt ggctatcggt atgtttggta
     8101 atctgggtac agctgggcaa ggtttgagtg cctttatcaa gtcggcttgg accaatattc
     8161 aatcagctat tcagacggct gggaatatca tccgaacggt cattgactgg atcaagttgg
     8221 ctttttctgg tgctggaaat gctgttggtg tgcttaagaa tgtcttttcc ttggcttgga
     8281 tgggtattca agatgccatt caagttgcga aaggggtcat tgatggtgtt atctctggca
     8341 tcaagggtgc ttttacaagt tttcaaagcg ttgtatctag cgtcggcggt gctgtcaatg
     8401 gggttatcac taatgttatt tctaccatta gaggaattgc taatattgac ctttctgggg
     8461 caggttccgc catcatgaat ggcttcttgg gtggtttgca gtctgcttgg gagggtgtca
     8521 agagtttcgt aggcggtatt gctggctgga ttgcggaaaa caagggtcct atctcatacg
     8581 accgtgtact tttgaaacct gctggtcttg ctatcatgca aggtttaaac accaacttgc
     8641 aagtgggatt caaggacgtc atgggaacgg tatccggtat ggctggagca attgctaagc
     8701 cttttgaaaa tcaatctttg gcctatgata tgacgtcaag tgcatcggtg gatgtacgtc
     8761 gaaacttgct gtcttctact ggtgatttgg ctggaaatga tggcggaaat agtttagcta
     8821 ctcgtctggc gaacatcgag cgattcctat ctgctttggt ggataaggag ttggcagttt
     8881 atctggacgg tgagaaattg gctcagaata gttacatgca tcaaggagcg attatggcaa
     8941 gggagggtat ttaatgaact atatgattat caatggctta gatacttcga ccttggcaga
     9001 ttgccatgtt cttgactttg gtaaagcaca aacttcagtc gagcggtctg aacaagtcga
     9061 ggtcttcggt gccaatggtc aactacatgt cagcgagggt gcgtatgatg gctacaacag
     9121 gacatttatc atcacgctac gacatttggc agatgctatg cgcttgattg aggtttttca
     9181 gtcggaaaat aatacagtag aattcggcta tctgagggat agcatttttt actgtaattt
     9241 ggtatctagt agctacgtgc cacttggtcc acatcgttgg aaggtggaaa ttacggtgtc
     9301 catgcatccg tttcggtatg ttaaaaatcc agctgacgtc gttttgacct catcaggttc
     9361 tgtgcagaat ccaggtacgg tctattctga acctatcatc atcatagagg gttcgggacg
     9421 ggtgaccttg accattggtc agcaattgat ggagttagaa ctagatactc gtgcgaccat
     9481 cgattgtcgg cataagcgac aaaatatcta tgacaagaat ggtgctgtaa agaacaccat
     9541 tcgcaaacgt ggtccattct ttgagatagc tgttggaaga agcggtattg caacaagtgg
     9601 aactgtctca aaaataacaa tcaaagggaa ttggaggtac aaggtttgat ttatctaaaa
     9661 gacggtaata tcccgcttaa tcttgcttac gatgatgaca tcgtgcagga ggccaatagc
     9721 acctaccaac tttcctttaa atttccgctg actgatggga agtggaatct gcttagacgg
     9781 gaagtttttc tgctggctga tgatctacac ggtgaacagg aattttttat ttttgaagta
     9841 aaaaaagcca aaggtcatgt gcaggtctat gctaagcagg tcgcaacatt attgaattac
     9901 tactctatca actctatttc ggttgacagg gtaccagggc agacggttat gactgctttg
     9961 gcaggtagcg ttaaacgacc atgtccattt acgtttttta gtgatatatt agaccgtcat
    10021 acgtttaatg agtccaatgt atctgtaatg gctgctttgg ccaaggataa acactctatt
    10081 gtcggtcagt ggggtggtga cttggtgcgt gacaagtacc aggttaaatt gttgaaaaat
    10141 ggcggcattg agaatgagtc gctatttatg tataaaaaga atctcagtag ctatgaagag
    10201 tctgagaaca ttaacaactt aaagacacga ttgcatctca aaaagacgat caaaggacag
    10261 tctgagggtg aggcagaccg tgtaattgca gtgactgtgg atagtccgtt gattgggcaa
    10321 tatcgtcaaa tctacgaagc agatattgag gtcaatgacc aagatgtgac agatgtggct
    10381 agcttaactg cctacgggaa acgttatttc agttcaacac tttgcgattt ggttgaaaat
    10441 tctatcaatc tggatgtcaa aggcaagtct gatgtatctg ttaagatgtt cgacatggta
    10501 agtgtattcc acgagcgatt cgatgtggat ttgcgtttga aaatttctag ctaccatttc
    10561 ggaccgatgt ctaagcgatt gaagtcaatt ggttttggca aggtgtcgca gtcatttggc
    10621 tcgacagtag cgagcatggt cgctggcagt gttgataaag caactggaag attgtcagca
    10681 tcctttgaac agaaactgca gaaggaaatt gacaatgcaa accgccattt cgacgctgaa
    10741 ttcgacaagc gagttgaatc catcaatgac ggtatcgagc aagcccaagc tgaggctgag
    10801 cggtatgctg acgctattaa acaggaaatt gatactgaaa tcgcccaagt caaccaatcc
    10861 atgcaagcac aatccgagga acacgacaga caggtcgcag acatcttgtc caagactcaa
    10921 tctgtcgaat cacttgccaa ccaggcaaag tcggatgctg ctagtgcatt ggctagagct
    10981 aaccaggtaa agaccgaggc tatcgcagat gcgagagcgc aggtagcgac agtcagtcaa
    11041 gccttaaaca ctgccaagtc cgaactgcag acggctatcg ctagtgctga ccaaaaggcc
    11101 agagatagcc aagcgagtgc tacagctttg cgaaacgacc tcaacttgca agctagcaag
    11161 attttggagc aagcacgagc acagacagct ttgaccaatc gtgtgacgac cgttgaaacc
    11221 ttggcagatg gtacgaggtc aacagtcgca gaactctcta aaacggttaa taaagcgaca
    11281 ggggacatca ctagtgttac agctaggacc aagacagtag aagacactct gagccaaacg
    11341 aggacccaat acgaagctct aacacagacc gtcaatactc agacaggaca gattgagagt
    11401 atcaatcgaa agactgctga cttgcagagt ggcatagatg gcgtgacgga gcggtttgag
    11461 aatctgaggg ttggtgggac taacttattc aaaaatagtg atttcagtca aggggaaaaa
    11521 aattggcata aattgcctga aatccataat gaagctacag gtaagtatgt aagattgcca
    11581 gctcgtcttt ggacaatggc acaatttgtt gaagtcgagc aaggtgaaga ttatgttatt
    11641 agcctttatg caaagaaaat atcagaaagc agttctagtc cgagattaaa tataaaattt
    11701 gattcgttac ataacgaaga tactgattat gttgaaatta caaaaactga ctggaaacga
    11761 tttattttta aatttcgtgc aaaaaaatcg ggtaaagaat tagtatattt tttaaatcga
    11821 aatggtatcg aagtcgatat aaaaaatata aagatggaaa aagggctttt agcaacagac
    11881 tactctccat cttacgaaga ctatcgctct gaaatcgcaa catacaaacg taccgccgag
    11941 gaatccagtg cagagttgtc ccgtcaaatc caattggcag atggcaaggc tgtcgaggct
    12001 aagacctacg cccagcagac tgctgaaggc tttaagactc gtctggagag cctagaaagc
    12061 tacaaagatg cggaaggtac acgagccaac caatatttga ctgccagtcg aaccgaaact
    12121 gccaaacagt tatctgccga acgtgccgcg attgctacta actatgtggc taagtctacc
    12181 tacgacgaaa atgtcagagg aacaacgcta aagcttaacg agattaagac gaccgctgac
    12241 actgccaagc aaaatctagc gacttatcaa aatacagtcg ataggaagct agaagaattg
    12301 accacaagca ctcaaacact tgacgggaaa atcaacactg caagtgccaa ggtcgatacc
    12361 gtggcgggac agatacggac tgagattagc gaggttgagg ggaagatacc tatcacagcc
    12421 ggaacacgta acttattaaa aggcactaaa gagctgactg atatatattg gacatcgaat
    12481 gtaacaagtg attactatca aggatttagg atcgccagaa cagcaccatc ggcagcaaca
    12541 tatattgaca cttatagagc gtcaacaaca attgttcctg atgccacgga atacataatc
    12601 agtttttacg caaaatcctc gattaatggc acaccgatta acaaccactt ttatagtcca
    12661 aacacgacaa caagaagcga aagtaacaca gggtacatcg gtaaagggac agatggtctt
    12721 gccattatca atctgacaac aacttggcaa cgctactgga taaaatggac gcagacacca
    12781 tctaacacta agaaaaacgt gataatcgga cgtaatttta gcgcaaataa tgcaacagtt
    12841 gaaattgctg gggtggctct atacgagggt agcttaaata aggattggtc accagcgcct
    12901 gaagattttg cgaatgaact atcatctgtt aaaaccacaa tcactcaaac cgcatccggt
    12961 gtcgagcaat tatcgactag cctgactacg actgatagca aggtcacgac tgctgaggct
    13021 aaaatccgac agttaattag cgatgtgtca agcaaagtat ctcaaacgga ctacaatacg
    13081 ttgactggcc gagtcgatag cgctgaaaca gcgattactc aaaatgcgac agagattagc
    13141 aagcgattga caaagacgca ggttgataaa gctatcacag ataaaggcta tcagacaaag
    13201 tctgatgttg acagcaacat cacaggtcgt ggctatatta ctaatagtgc attgcaacct
    13261 tatgccttgg ctacaacagt ccaaaacctc gtcagagaga ctgcggatag ttttagccgg
    13321 acgataagtg agactaaagc gctgattccg acatcggtag gtggacgaaa tctgattaga
    13381 ggctcgtctg aaatgattat aggcagtggt cgttggcaag atggcacatt tcgaaaatct
    13441 ggtacaggta atattaagac aatcaacatc agtaatccgc ctgtcccaaa tgtgactaaa
    13501 ggaatagaag ttgaaattgc gtcagctacc gaatgtggga ttgcccaaga cacactattt
    13561 ttaccaaagg gcacctacac attcactgtt tgggtcaagg gcccaactgg agccactgga
    13621 cgaattgata cgtttaatgg tagcgggtca aaatcaaaag tgtttaaatt gacaggcggc
    13681 tgggataaag taacagtcac taatacatcg acagtagacg aaactaaaaa tgtcggttat
    13741 gtatatctac aaaattctcc cggttaccct gcgaaaatgc atttaacagc tccaaaactt
    13801 gaggcaggaa ccattccgac ggattataca ctagccattg aagacttggc cactgtcact
    13861 gccctacacg atgtcaaaga tacggtatca agccacaccc gtactatcgg cgctgtcggt
    13921 gagacaggca gtattttgga caacgtcagc aaagttactc agactgcagc gggtttggtg
    13981 caggaagtat ctggtgataa tggactcaag acacaagtca gtcaattggc tgggtcttat
    14041 gcgattaaaa atttgaccag ctcaggcaag gtacttaacc agctcaacct aaataaagac
    14101 ggttcggtca agattgacgg cagtctcgta cagattactg gtaggactta catacaagac
    14161 ggtgtgatta catccgctaa gattgcaggc ttagatgctg gtaaggttac gacgggttat
    14221 ttagcatctg cacggattaa agcgaatagt atcgatgggt caaaaattgc attcgatgaa
    14281 gctttcttca acaggttagt cgcgaaccaa gcctacctga agaagttatt tgccaaggat
    14341 gcatttttga cagcagtgca agctgtaagt ttgtcagcga gccaaatcat aggtggattg
    14401 atgagggcaa ccaatggagc tatggagatt gactttaata attcggctat tgatatgttt
    14461 aaagatggcg cgataagatt tcactcaggc tctaatgcta tctatcgtca aagtacagat
    14521 ggcatccata cagcgtttgt gcatttcgac acaacatcca agggggggat atatgcttct
    14581 cttggttcga catcatcaag ggatggtatt aacagtaagt cttcaggtcg ttttgccggt
    14641 atccgagtgg ctcggacctc tgagcgttca gattctcatg ttgcgtcgca ggatgctgtt
    14701 gaactgtacg gagatacaat tttcttcggt catggtttcg aaggcggagg tttctcgatg
    14761 catacaactc tgacgggcgg ccgaacgatt agtctaaaca agatgcatga cgcactcatt
    14821 aacctttgtc gctgctggat acacttgcga aacgaaggtt ttaatttgaa tagttcagca
    14881 ttcaggaagt cgttagatac agaaattcat aacgcacaac aactttataa tacaatcgaa
    14941 aggattacat aatgaaccaa gaactaatca accaagcgct acgcttgact aacaatgacc
    15001 tcatgactaa attgtcagag gaaatgacga ccaagaactt gctcgctgtg caactaaccg
    15061 aagcgcagca gacgattgcc aatttgcggg cagaaattac tgagttaaca aagcaactgg
    15121 acgaagctac taaaccagaa gaaatcattg aacaaaaagg agaataatca tgactcaaac
    15181 tactaacaac acattgctta atttggaaga aacaacacaa ccatttgacc ttgcgactgc
    15241 actccgctac atgaaagaac atggtgaatt tatccgctgc aagtcagcga cacaagactt
    15301 ttatatgtat cgcgacgtgc agaaacgtcc tgcaattgtg aatggtcgtc gcaagtttgt
    15361 tgacgtggaa acgatttggg catttaacca gtggggtagt actacgacga ctatcaacat
    15421 tgctgatatg ctcaacgaag agtattggat tatgaaattt gacgaacatg gcaacccaga
    15481 ctggtcagac ccgacagttg gggcggaggt gtaatacatg gttatgatgt tatctaaaac
    15541 gccaccacat cctgcaggaa tgtttgaatt tttacgcgag ctaatcgcaa cagaggacgg
    15601 tctagtcctc tttttgcttg ggcttattgt taccatggaa attgtcgatt ttttatcagg
    15661 tacattcgct gctatgatta acccagacat cgaatacaag tctaaaatcg gtattaatgg
    15721 cctgattcgt aaaatgatgg gaattatttt gcttaccgtg ttgataccaa tgtcggtctt
    15781 gttgcctgaa cagacgggtg tggcatttct ctacacaatc tacgttggct atctcatctt
    15841 gacctttaag agcttgatcg agaactacgg caaagccaag ggcgacacct caatatttga
    15901 aaacgtgaca gcagcttttg aaaagttgat tggtaaaggc aaatgaaacc aatcaaagca
    15961 atcttgatta ttattgtttt gggagctatg acgcctattt tgtgggtgct agctcctttc
    16021 tattttgaaa aggaagataa atatgacaac agtaaatgaa gccttaaata acgtccgtgc
    16081 ccaagtcggg tctggcatat cagtaggtaa cggcgaatgc tacgctttgg ctagttggta
    16141 cgagcgcatg attagtccgg atgcaactgt cggacttggc gctggtgtgg gctgggtcag
    16201 cggtgcaatc ggcgatacaa tctctgccaa aaacatcggc tcatcataca actggcaagc
    16261 taacggctgg acagtttcca catctggtcc atttaaagca ggtcagattg tgacgcttgg
    16321 ggcaacacca ggaaaccctt acggacatgt ggtaatcgtc gaagcagtgg acggcgatag
    16381 attgactatt
//
LOCUS       DAZQCJ010000038        16191 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000038 DAZQCJ010000000
VERSION     DAZQCJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16191
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.038"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(48..2699)
                     /locus_tag="ACHU66_001122"
     CDS             complement(48..2699)
                     /locus_tag="ACHU66_001122"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR3389414.1"
                     /translation="MSKELSPKYNPAEVEAGRYQTWLDQDVFKPSGDAEAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQG
                     VTRYDLGREKFLDKVWEWKDEYAATIREQWGKLGLSLDYQRDRFTLDEGLSKAVRKVF
                     VELYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALQVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIVNKPIPIVADEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAGEFAGMDRFEARKATVAKLQELGALV
                     EIENRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTADKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNESGEMYVGEEAPAGDGWVQDEDVLDTWFSS
                     ALWPFSTMGWPDENAADFQRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGSQPFKNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDAARENVAKVAAGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEAEKVMTRSVLLYTLDQI
                     LRLLHPIMPFVTEEIFAQYAEGSIVVAAYPVVNPAFENAEAHKGVESLKDLIRSVRNS
                     RAEVNVAPSKPITILIKTADSELETFFKANENYIRRFTNPEQLEISSSIAAPELAMSA
                     VITGAEIFLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFIANAKPEVVEK
                     EKEKQADYQAKYDATVGRIAEMKKLVK"
     gene            complement(2875..3306)
                     /locus_tag="ACHU66_001123"
     CDS             complement(2875..3306)
                     /locus_tag="ACHU66_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389415.1"
                     /translation="MKSLTQTFTKKQKLVVVIVVILSVAIGFWKWRDTFFNTYYPDDT
                     SIQGQLFPNVANTKIETVENVLPFSDVKYDKIIIRIGSLSSEYDDSNQSSKANNIINR
                     KTLDENFIQWLIEAFDEEEAKKISDHIEVYYRGEIISEISY"
     gene            complement(3303..4076)
                     /locus_tag="ACHU66_001124"
     CDS             complement(3303..4076)
                     /locus_tag="ACHU66_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389416.1"
                     /translation="MKMLNKFTENSKDLRNELTSWFVIISLAFLLFLSIPWLVQKTLY
                     FTLSKPFEAQRVAITKSNKTSDTASGVEGTYINNIPFNDFEKLMNFSNVSFKSVDIQI
                     PRIYITSPIFNTSEYGDQLQDIWIDSLNKDELEEEFGSYKVYYDGNLIIENGASYTSE
                     GATKNQIISMKETIVTELDTNKEFKKYTFKKYYIPDEGTDPTEIKIIEDGFAIETVFE
                     QKDLIPNIAFGPTLYTVRAEFIKENNKLVLRKGSMEVYK"
     gene            complement(4240..4815)
                     /locus_tag="ACHU66_001125"
     CDS             complement(4240..4815)
                     /locus_tag="ACHU66_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFR3389417.1"
                     /translation="MNLVIIGAQASGKMTIGQEVAKLTGMTLFHNHDSIDFSLRFIPE
                     FSEDMFDLNTRITFAVYDVFARSGRPLIGTALINFKNLIEVQFLTTVQTIFHYHGQEI
                     LFVELETALEERLRRNRTENRLTHKPLKRHIEVSEAEILSTADTCQYTSLGIPEGIQH
                     YLKINNTHLTANEVAQLIIKKMEELEQKQTK"
     gene            complement(4812..5201)
                     /locus_tag="ACHU66_001126"
     CDS             complement(4812..5201)
                     /locus_tag="ACHU66_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR3389418.1"
                     /translation="MLHHVEIYVSNLETSRAFYDFLLTKLGYSLYQEWEEGLSYKKAE
                     QYLVFVQTPQDFLKAGYHRCRTGLNHLAFHVGTPDDVDQWRKEFLTRRVKLLYNDRYP
                     YAGGPDHYALYLEDPDGIKIELVGEVT"
     gene            complement(5443..5829)
                     /locus_tag="ACHU66_001127"
     CDS             complement(5443..5829)
                     /locus_tag="ACHU66_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR3389419.1"
                     /translation="MAKKKVNRKKQLKKQLADLKRAGRVGLERAAEVVETVAHKAEAV
                     VEHAVEQVKEVVAEVTSSATSLEDFLALPELEGIAAARLETFYEAGIQSVADFANHTE
                     KELLALKGIGPATIKQLKEKGIELKA"
     gene            complement(5844..6098)
                     /locus_tag="ACHU66_001128"
     CDS             complement(5844..6098)
                     /locus_tag="ACHU66_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HFR3389420.1"
                     /translation="MTKEQEFLKEFEAWVNTQVMVNEMAVEESRRVLEEDKDERAADA
                     YIRYESKLDAYRFIQGKFANYHAGKGFHDLPDELFGQRHY"
     gene            complement(6206..7207)
                     /locus_tag="ACHU66_001129"
     CDS             complement(6206..7207)
                     /locus_tag="ACHU66_001129"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFR3389421.1"
                     /translation="MIEVKTFGGQAKLYCLENKNGMQVTLTDFGARVVEVLLPVEENG
                     GLRNVSLAAKSDDDYRKTDLYPGSSIVPVAGRISGAQAEIKGTSYQFTENEPGRTLHG
                     GIDTANEQYWDVALDHEKNQVTFGIVLKDGFNGFPGDVRVEAIYGLTDQNELTIDYRA
                     VSSKDTIFNPTNHIYFNLTGDFQQSVAEHEIRIAANRYAPLGEDNLPTGVLEDVTGTP
                     FDFRERARFAQGFDSQHPQNILVKGYDHPWLLEQVEVPIEVVSPDGKISLRVRTNQAS
                     VVIYTYNYPVEALAAYHGAFSLECQALPNACNLGGFGSILLEKDEEFLSQTVYQFTW"
     gene            complement(7423..7824)
                     /locus_tag="ACHU66_001130"
     CDS             complement(7423..7824)
                     /locus_tag="ACHU66_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR3389422.1"
                     /translation="MVRSLVLVSHGIFCEELKKSTEMIMGPQEDIYTVALLPEEGPED
                     FQKKFEETIAGLEDFVVFADLLGGTPANVVSRKLIEGGQFDLYAGMNMPMVIGFLNGV
                     LLGEAVDYVEFGTSNLVHVNSLLTSDEDDDE"
     gene            complement(7824..8777)
                     /locus_tag="ACHU66_001131"
     CDS             complement(7824..8777)
                     /locus_tag="ACHU66_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR3389423.1"
                     /translation="MAIIATRIDGRLIHGQVANLWTTKLNIGRIMVIDDAVAQNDIEK
                     QGLKLACPPGVKLSILPIEKAANNIKEGKYDSQRLLIVARRPENFLRLVEYGVELPEL
                     KEMMKLHTQFFNTSPFFHTIITGIDLALEESDGVASKDAVNGVKTGLMGPFAPIGDSI
                     FGSLVPAIMGTVAATMGKEGSPVGIFLWVAVAIAYDIFRWKQLEVAYKEGTKLITTMR
                     DRLTALVDAASVMGVFMMGALIATMINFEVTWAPAIGEKVIDIQDILNTIFPRLVPAI
                     FTGFVFWLLGRRGMTSTKAILIIIVLALGLSAIGHFLFGMV"
     gene            complement(8831..10603)
                     /locus_tag="ACHU66_001132"
     CDS             complement(8831..10603)
                     /locus_tag="ACHU66_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFR3389424.1"
                     /translation="MKEFYIGDQFYLDGEPFKILSGAIHYFRVHPDDWYHSLYNLKAL
                     GFNTVETYVPWNMHEPRKGEFCYEGILDIERFLKLAQELGLYAIVRPSPYICAEWEWG
                     GLPAWLMKEELRVRSSDSVYLQHLDEYYASLIPKLAKLQLAQGGNVLMFQVENEYGSY
                     GEEKEYLRSVAGLMRKHGLTAPLFTSDGSWRATLRAGTLIEDDVFVTGNFGSKARENF
                     ANMTAFFNEHQKNWPLMCMEFWDGWFNRWGDEIIRREPEEMVDSVMECIELGSLNLYM
                     FHGGTNFGFMNGCSARGQIDLPQVTSYDYDAILDEAGNPTKKFYILQQRLKEVYPELE
                     YAEPLVKEAKAFSDVSLHDKVSLFATLENVSGCVKGFYPKNMEELDQSTGYILYRTEL
                     ERDKTEAERFRVVDARDRIQIYADGKFVATQYQTEIGDDVELDFKDDKLTLDILVENM
                     GRVNYGHKLTAPTQSKGLGRGAMADLHFIGHWETYPLPLDSVEDLDFSKGWEEGQAAF
                     YRYQFELDELADTYLDMTGFGKGVVFVNNVNIGRFWEKGPILYLYIPKGYLKKGANEI
                     VVFETEGKYREKIHFSQRPVIKDL"
     gene            10810..11520
                     /locus_tag="ACHU66_001133"
     CDS             10810..11520
                     /locus_tag="ACHU66_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR3389425.1"
                     /translation="MKVPKYQLIQNDLRQQIVSGKFENGDKFYTESELTKLFNVSSIT
                     VIRAVNELVKDGYLVRQQGKGTFVSRSRKGRLVEFSDIEIFPMDKDKVTVLSCEKGNK
                     PDILEKLNLDKNEFYYKIVRVRAAEDTPYIFHNSYIPQRYIQNPDAPLEHYQSIYQRF
                     KLDYNIHMSEEPFVETNEIVSPCPKEVATHLKLKATEPAVLQNKTTTNSTSGEVMEYT
                     ETYKHWKYYKFEITANHR"
     gene            complement(11660..12391)
                     /locus_tag="ACHU66_001134"
     CDS             complement(11660..12391)
                     /locus_tag="ACHU66_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR3389426.1"
                     /translation="MVSQTVEKPLYLQLVDELEVAIRERMAPNDKLFSERELTQVYGV
                     SRITVRLALQELEKRGLVYKKHGKGTYVSEISDTAVDLSQAYSFTEQMKKIGKVPRTS
                     ILSFELVKASDYIAQHLQLSQGEEVFEVERLRLADEVPMMLERTYVPASLFPGLAAQR
                     MKSTPLYEIFSEDYHQVIRLAEEEFYASIALDNEAKILGIPSNSPVLHLVRKTYNDKN
                     RIIEFTFSIARADQFRYKITHQRGY"
     gene            complement(12491..12808)
                     /locus_tag="ACHU66_001135"
     CDS             complement(12491..12808)
                     /locus_tag="ACHU66_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFR3389427.1"
                     /translation="MAFEQTNGRYASFGVVTSLPGEVIDSFWYVIDHYLKGVIPLKSV
                     IRFSIKNRRGKITLVFSQEGYKNVLAVDLSSRFDPFYPSTILVMDKQGKETITLPDEV
                     TLL"
     gene            complement(12866..13399)
                     /locus_tag="ACHU66_001136"
     CDS             complement(12866..13399)
                     /locus_tag="ACHU66_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFR3389428.1"
                     /translation="MKLPKEGDFITIQSYKHDGEIHRTWRDTMVLKTTENAIIGVNNH
                     TLVTENDGRRWVTREPAIVYFHKKYWFNIIAMIRENGVSYYCNLASPYVLDNEALKYI
                     DYDLDVKVFADGEKRLLDVDEYERHRKAMKYSDDIDFILKENVKILVDWINNQRGPFS
                     PAYVNIWYKRYLELRSR"
     gene            complement(13475..14251)
                     /gene="recX"
                     /locus_tag="ACHU66_001137"
     CDS             complement(13475..14251)
                     /gene="recX"
                     /locus_tag="ACHU66_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR3389429.1"
                     /translation="MRITKIEKKKRLYLLEVDGQDSLYITEDTIVRFMLSKGKEITEQ
                     EFRELRDFAQFSYGKNLALYYLSFKQRTKKEVSDYLKKYEIEENNIVKIVMVLEEEKW
                     LDDGNYVDSYVRQNALNGDKGPAMIRQKLMQKGIPKPLIDKRLAEEDFSELAGKIGEK
                     LVGRYQRKLPLRALQDKVVQGLMGKGFSYETAKQSLSQLELVADEENEDDLIAKELDK
                     QYRKYSRKYEGYELKQRLIQALARKGYDFDRIQAVLRDYL"
     gene            14289..15641
                     /gene="rlmD"
                     /locus_tag="ACHU66_001138"
     CDS             14289..15641
                     /gene="rlmD"
                     /locus_tag="ACHU66_001138"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR3389430.1"
                     /translation="MNLQVNQRIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEEIFC
                     QITSVKRNFVEAKLLKINKESKFRVKPACDIYQACGGCQIMHLRYDKQLDFKQDLLQQ
                     ALKKFKPAGYENYTIYPTIGMDKPSHYRAKLQFQTRSFKDQVKAGLYAENSHRLIGIT
                     NCYVQDAETQEIINGIAKLLTKHRIPIYNERKQHGIRTVMVRKARKTGQVQVIFITSC
                     QVNLIKLIDELTSQFPSIITIALNWNRQKTSDIYGEKTEILWGEQAIDEAVLDYEFSL
                     SPRAFYQLNPQQTEILYGEAVKALDISGDEDLIDAYCGVGTIGFAFAKKVKSVRGMDI
                     IPEAIEDAKANAKRMGLSNTHYEAGKAEEIIPKWYQEGYRADALVVDPPRTGLDEKLL
                     KTILDYKPAKMVYVSCNVSTLARDLVELSKAYKVEYIQSVDMFPHTARTEAVVKLSKK
                     "
     gene            15937..16128
                     /locus_tag="ACHU66_001139"
                     /pseudo
     CDS             15937..16128
                     /locus_tag="ACHU66_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4813 a         3771 c         2836 g         4771 t
ORIGIN      
        1 accacaaaac ccagccattt taggttgggc tttttatcgt attctaccta cttaaccaat
       61 tttttcattt ctgcgatacg tcctactgtc gcatcatact tagcttggta gtcggcttgt
      121 ttttcttttt ctttttcaac gacttctggt ttggcgttgg cgatgaagcg ttcgttgctg
      181 agttttttgc cgaccatgtc tagttctttt tgccacttgg caagttcttt gtctagacga
      241 gccaactctt cttcgacgtt gaggaggtcg gcgagtggca agaagatttc agcaccggtg
      301 ataacggctg acatggctag ttctggtgcg gcaatgcttg agctgatttc caactgctct
      361 gggtttgtaa agcgtcggat gtagttttca tttgccttga agaaggtttc aagctcgcta
      421 tctgccgtct taatcaagat ggtaatcggc ttagaaggag caacgttgac ctctgctctg
      481 ctatttcgca ccgaacgaat cagatcctta aggctttcca ctcccttgtg agcttcagcg
      541 ttttcaaagg ctgggttgac aacagggtaa gctgccacca cgatagagcc ctctgcgtac
      601 tgagcgaaga tctcttccgt cacaaacggc atgatagggt ggaggaggcg aaggatttgg
      661 tccagcgtgt agagaaggac agagcgggtc atgactttct cagcctcgtt gtcgctgtaa
      721 agcacctcct tggtcagctc cacataccag ttggcgaact cttcccagat gaagttgtag
      781 aggatgtggc cagccacacc aaactcaaac ttgtcgaagt tttcagtgac cttagcgatg
      841 gtttcgttaa ggttgtggag aatccagcgg tccgtcacgt tgccagcctc accagctgcg
      901 actttggcaa cattctcacg cgccgcatcc aaagtcaagc cttcattgtt catgaggatg
      961 tagcgggaaa tgttccaaat cttgttgatg aaattccaag aagcgtccat cttctcataa
     1021 gagaaacgaa catcttgacc aggggcagag ccatttgaca agaaccaacg caaagcgtcc
     1081 gcaccgtatt tctcgatgac atccatcggg tcaatcccgt ttccgagtga tttagacatc
     1141 ttgcgacctt cttcgtcacg aatcaaacca tggatcagca cgttcttgaa tggctgactg
     1201 cctgtgaact caagcgattg gaaaatcata cgagacaccc agaagaagat gatgtcgtag
     1261 cccgttacca aggttgaagt tgggaagtaa cgctggaagt ccgccgcatt ttcatcaggc
     1321 cagcccatag ttgagaatgg ccaaagggca gagctgaacc aggtgtctag gacatcctca
     1381 tcctgtaccc aaccgtcacc tgctggggcc tcttctccga cgtacatttc gccagattcg
     1441 ttataccatg ctggaatctg atggccccac caaagctgac gagaaatcac ccagtcgtgt
     1501 acattttcca tccattgaag gaaagtatcg ttgaaacgtg gtgggtaaaa ttctacctta
     1561 tcagctgtgt cttggttggc aatggcattc ttagccaatt ggtccatttt aacaaaccat
     1621 tgagttgaca aacgtggctc gactaccaca cctgtacgct cagagtgtcc aacagaatgc
     1681 acacggtttt cgatttccac aagggctccc agttcttgca acttagcaac cgttgcctta
     1741 cgagcttcaa agcggtccat acctgcaaat tcgcctgcca actcgttcat agtaccatcg
     1801 tcgttcataa cattgacttg tggcagattg tggcgttgac ctacgaggaa gtcatttgga
     1861 tcgtgggcag gtgtgatttt cacaacccct gtaccaaatt caggatccgc gtgctcatcc
     1921 gcaacgatcg ggattggctt gttgacgatt ggtaggataa catttttgcc aatcaagtcc
     1981 ttgtaacgtg ggtcttctgg gttaaccgcc accgcaacgt caccgaacat ggtttcagga
     2041 cgagttgtcg caacttgaag ggcacgagaa ccgtcttcca acatgtaatt catgtggtaa
     2101 aaggcacctt ctacgtcctt gtgaataact tctatatctg aaagggctgt gcgagctgca
     2161 gggtcccagt tgatgataaa ttcgccacgg tagatccagc cttttttgta gagttcaaca
     2221 aagaccttgc gaacagcttt tgacaagcct tcgtccagcg taaagcggtc acgttggtag
     2281 tccaaagaca gacccaactt gccccattgc tcacgaatgg tcgctgcgta ttcatccttc
     2341 cattcccaga ctttgtcgag gaatttctca cggcccaagt cataacgagt cacaccttgc
     2401 tcacgcaagc gctcctcaac cttagcctga gtggcaatcc ccgcgtggtc catacctggc
     2461 agccagaggg tgtcaaagcc ctgcatacgt ttctggcgga taataatatc ttgaagggtg
     2521 gtatcccaag catgtcccaa gtgcaactta ccagttacgt ttggtggcgg aatgacgata
     2581 gaatacggct tagcctccgc atcgcctgac ggcttaaaga catcttggtc caaccaagtt
     2641 tggtaacgac cagcctcaac ctcagctgga ttatattttg gtgataattc ttttgacatt
     2701 aatttttctc ctttagtatt ctttaagaaa attggtttat actatcctca agctagaaat
     2761 agcaaaactt ttctttttca tataatctcc tagaaatagt cagtcctttg ggctggctat
     2821 ttcgtttttc attcttgaac cctgcatcta cgggcgagag aggtttgtgt aacgttaata
     2881 actaatttcg cttattattt cgccacgata gtacacttcg atatgatcag atattttttt
     2941 agcttcctct tcatcaaatg cttctatgag ccattgtatg aaattttcgt ctaatgtttt
     3001 tctgtttatg atattatttg ctttcgatga ttgattagaa tcatcgtatt ctgaagacag
     3061 gctaccaatt ctgataatta tcttatcata tttcacatct gaaaatggca agacattctc
     3121 aacggtttca atcttcgtgt ttgcaacatt gggaaacaac tgcccttgga ttgaagtatc
     3181 gtcaggataa tacgtattga aaaaagtatc acgccatttc caaaaaccta ttgctacgga
     3241 tagtatcact acaatcacca ctactaattt ttgtttcttg gtgaaggttt gtgtcaaact
     3301 tttcatttgt atacctccat gctccccttc ctcaatacca gtttattgtt ttcctttata
     3361 aactccgctc tcactgtgta cagagtagga ccaaaagcaa tattagggat caaatctttt
     3421 tgttcaaaca ccgtttcaat agcaaatcca tcttctataa tcttaatttc tgttggatct
     3481 gtaccctcgt ctgggatata atatttttta aatgtgtatt ttttgaattc cttgtttgta
     3541 tctaattcag tgacaatcgt ttctttcata gatattattt gatttttagt agcaccctcg
     3601 cttgtatagg atgcaccatt ttcaataatc aaattaccat catagtaaac tttgtaggaa
     3661 ccgaattcct cttcaagttc atccttattt aaagagtcta tccaaatatc ttgtaattgg
     3721 tccccatatt ctgaagtatt aaaaatagga ctcgtaatat aaattcttgg tatttgaata
     3781 tcaacacttt tgaaagacac attgctaaaa ttcatcaatt tttcaaaatc attaaaggga
     3841 atattattga tgtaggtccc ttcaactcca gaggcagtat ctgaagtttt gtttgatttt
     3901 gtgatggcta ctctttgtgc ttcaaatggt tttgataaag taaaatataa agtcttttgc
     3961 accaaccaag gaatgctcaa gaataataag aaggctagcg aaatgatgac aaaccagcta
     4021 gtcaactcat ttctcaaatc ttttgaattc tctgtgaact tgtttaacat tttcatagac
     4081 agtatactcc taattcatat tttaatacac tgcacgcaaa atagtatgct ccatgagata
     4141 agcatgatgt aatatagtag caggactgaa ctgatagtac taatagaaat agtttatgcc
     4201 tatctggatt atattctgtt tcgcccctca acggaaaggc tatttcgttt gtttttgttc
     4261 aagttcctcc atttttttga taatcagttg agcaacttca ttagcagtaa ggtgagtatt
     4321 attaattttg agataatgct gaatgccctc tggaatgcct aatgaggtat attgacatgt
     4381 atcagctgta gagagaatct cagcctctga aacttcaatg tgtcgtttca aaggtttatg
     4441 ggtcagtcga ttctctgtcc gattacgccg cagacgttct tccaaagcag tctccaactc
     4501 aacaaataaa atttcctgac catgataatg aaaaatcgtc tgaacagttg taaggaattg
     4561 aacctctatg agatttttaa aattaatcaa ggcagttcca attagtgggc ggcctgacct
     4621 agcaaacaca tcataaacag caaatgtaat acgtgtattg aggtcaaaca tatcctcaga
     4681 aaattcagga ataaaacgta ggctaaaatc aatcgaatcg tgattatgaa aaagggtcat
     4741 ccctgtcaat ttcgcaactt cctgcccaat ggtcatcttg ccagaagctt gtgctccaat
     4801 aattactaaa ttcatgttac ctcccccacc aactctatct taatcccgtc cgggtcttcc
     4861 aaatagaggg cgtagtggtc tggtcctccc gcgtagggat agcggtcatt gtaaagcaat
     4921 ttgactcgtc tggtcaaaaa ttccttccgc cattggtcaa catcatcagg tgttcccaca
     4981 tgaaaagcta gatgattgag acctgtgcga cagcggtgat aacctgcctt taagaagtcc
     5041 tgtggcgttt ggacaaagac caagtactgc tctgcttttt tataagatag cccttcctcc
     5101 cactcctgat agagcgagta tcccagcttg gtcaggagaa agtcgtaaaa tgcacgcgag
     5161 gtttccaaat tagaaacgta aatttcaaca tgatgtaaca tattttctcc tttccaaatg
     5221 aaaatccccg cctagacttg ctagacaggg acgaagttat tccgcggtac cacccttgtt
     5281 cagatacatt atctgcactc tgcttattcg attcacatca tcagcaacca gttttgacat
     5341 ttgtgcggat ttctcagtac caccgcttcc tgtgacaaat ggagatcaaa acacctctga
     5401 atggctttat tgtaacaaat tgcaccttga aaagcaaggg atttaagctt tcaactcaat
     5461 ccctttttct ttcaactgct tgatagttgc aggaccgatg cctttaagag ccaagagctc
     5521 tttttctgta tggtttgcaa aatcagccac agattgaata cccgcttcgt agaaggtttc
     5581 caaacgtgct gccgcaatgc cttccaactc aggaagtgcc aaaaagtctt ccaatgaagt
     5641 cgctgaagaa gtcacctctg ctacaacttc tttcacttgt tccacagcat gctctactac
     5701 agcctcagcc ttgtgagcaa ctgtctctac tacctctgca gctctttcaa gacctacacg
     5761 acctgcacgt ttcaagtctg ctaattgttt tttcaactgt ttttttcgat ttactttttt
     5821 ctttgccatg ttaactcctc tccttaataa tgcctttgac caaacaactc atctggcaaa
     5881 tcatgaaaac ctttgccagc atggtagttg gcaaatttcc cttgaatgaa tcgataggca
     5941 tccagcttgc tctcataacg aatataagca tccgctgcac gctcatcctt gtcttcttcc
     6001 aagacacgac gactttcttc aaccgccatc tcgtttacca tgacctgagt gttgacccaa
     6061 gcctcaaatt cttttaaaaa ttcttgttct tttgtcatta ttttcctctt tcgtctgctt
     6121 cgtttgaaac acaccggttt attataccat attttttaga aaacacaaga aaagcctaac
     6181 tgcaacacag ttagagctct ccttcttacc aagtaaattg ataaacagtc tgggacaaga
     6241 attcctcgtc tttttccagc aaaatagaac caaaaccgcc taggttgcaa gcatttggca
     6301 gagcctggca ttccaagcta aaggcaccat ggtaggcagc caaagcttca acaggataat
     6361 tgtaggtgta gatgacaact gatgcctgat tggttcgcac gcgcagactt attttcccat
     6421 ctgggctgac aacttcaatc ggaacctcta cctgttccaa aagccatgga tggtcgtaac
     6481 ctttcacaag gatattctga gggtgctggc tgtcaaagcc ctgagcaaat cttgctctct
     6541 ccctaaagtc aaacggtgtt cctgtcacat cctctaagac accggttggc agattatcct
     6601 caccaagcgg agcatagcga ttagccgcaa ttcgaatctc gtgctctgcc actgactgct
     6661 gaaaatcccc cgttaagttg aaatagatgt gattggtcgg attgaaaatc gtatctttgc
     6721 tagacaccgc ccgataatct atcgtcaact cattctggtc tgttagacca taaatagcct
     6781 caactctcac atctcctgga aaaccattga aaccatcttt taagacaatc ccaaaggtaa
     6841 cttgattttt ctcatgatct agagcaacat cccagtactg ttcatttgcc gtgtcaatcc
     6901 caccatgcaa ggtacgacct ggctcatttt ctgtgaactg ataactggtt ccctttatct
     6961 cagcctgagc acctgaaata cgccccgcta ctgggacaat gcttgaacca gggtacaaat
     7021 ctgtcttgcg gtaatcatca tccgacttgg cagccaaact gacattccgt agaccgccat
     7081 tttcttctac tggcaaaagc acttccacca ctctggcacc aaaatccgtc aaggttacct
     7141 gcatcccatt cttgttttcc aagcaataca gttttgcttg tccaccaaat gttttcactt
     7201 caatcatttc ttttcctcac aagccaaatg agggagtcac tattgcaact ccctcaggat
     7261 acttaaagcc tatcaactat agaacatttt ataattaacg aaaatcaaat ccgtctgtaa
     7321 caatcggctg agttagcctc actctcttat ttctaggctc gggctgtctt tggtcactct
     7381 ttcatttcaa ggtgaccagt tgaaacagtt ccccaaactg tttcactcat cgtcatcttc
     7441 atcacttgtt aatagactat tgacatgaac taggttgctt gtcccaaatt ctacatagtc
     7501 cacagcctct cccagcagga cgccgttcaa gaaaccgata accattggca tgttcatccc
     7561 tgcatagagg tcaaactgcc caccttcaat caattttcgt gatacaacgt tggctggtgt
     7621 accgcccaac aagtctgcga agacaacgaa atcttccaat cctgcaattg tttcttcaaa
     7681 ttttttctgg aagtcttctg gaccttcttc tggtaataaa gctactgtgt agatgtcctc
     7741 ttgaggaccc ataatcattt cagtagattt tttcaactcc tcgcagaaga tgccatgact
     7801 tactaatact aaacttctaa ccattatacc ataccaaata ggaagtgacc gattgctgac
     7861 aagccgagtg caagcacaat aataatcaag atagctttag tcgaagtcat accacgacga
     7921 ccaagaagcc agaagacaaa tcctgtaaag attgcaggta ctaggcgtgg gaagatggta
     7981 ttcaaaatgt cttgaatatc aatcactttt tctccaatag ctggagccca agtcacttca
     8041 aagttaatca tagttgcaat cagggctccc atcatgaaga cacccattac agatgctgca
     8101 tcaacgagag ctgtcaagcg atcgcgcatg gttgtaatca acttagtacc ttctttgtag
     8161 gcaacttcca actgtttcca acggaaaata tcataggcaa tggctactgc tacccaaaga
     8221 aagataccaa ctggagatcc ctctttaccc atagtagcag ccacagtccc cataatcgct
     8281 ggaacaagag aaccaaagat tgagtcaccg attggagcaa atggtcccat caaacctgtc
     8341 ttaacaccgt taaccgcatc tttagaagca acgccatcac tttcttcaag tgccaagtca
     8401 atacctgtga tgatggtatg gaagaatggt gaagtattga agaactgagt atgcaatttc
     8461 atcatttcct tcaactcagg gagctcaacc ccatactcta ccaagcgaag gaagttttct
     8521 ggacgacgag caacaatcaa gagacgttga ctatcatact tgccctcctt aatattatta
     8581 gcagcctttt caatcggtaa aatcgataat tttacgcctg gaggacatgc taatttcagt
     8641 ccttgttttt caatgtcatt ttgagcaaca gcatcatcga taaccataat acgaccgatg
     8701 ttcaatttcg tcgtccataa gttagcaacc tgaccgtgaa tcaaacgacc atcaatacgc
     8761 gtagcaataa ttgccataca gtgttctcct tttcttcata aatcttttga gcagaaaccg
     8821 attaaaattt ctacaagtct tttataacgg gtctttgtga aaaatgaatc ttctctctat
     8881 attttccttc tgtctcgaag acaactattt cattcgctcc tttctttaag tatccttttg
     8941 gaatatagag atagaggatt ggtccttttt cccaaaaacg tccaatattt acattattaa
     9001 cgaaaacaac tcccttacca aaacctgtca tatctaagta agtatccgca agttcatcca
     9061 gttcaaactg ataacgatag aaagcagctt gaccttcctc ccagccctta ctaaaatcca
     9121 ggtcctcaac tgaatcaaga ggcaaaggat aggtttccca atgaccaata aaatgtaaat
     9181 cagccatcgc acctcgacca agccctttag actgggtagg agccgttagt ttgtgcccat
     9241 aattgacacg tcccatattt tctaccaaaa tatctaatgt tagcttgtca tctttaaaat
     9301 ccaattcaac atcgtcacca atctctgtct gatactgagt tgcaacgaat tttccatctg
     9361 cataaatttg gatacggtca cgcgcatcaa ctactcggaa acgttccgct tctgtcttat
     9421 cccgctccaa ctctgttcta tataaaatat aacctgttga ctgatcaagt tcttccatat
     9481 tcttaggata aaaccctttt acgcaaccgc ttacattttc tagagtagca aataaactca
     9541 ccttatcatg aagcgacaca tctgaaaaag cttttgcctc ttttacaagc ggttctgcat
     9601 actctaattc aggatagact tctttcaaac gttgttgcaa aatatagaac ttcttagtag
     9661 gatttccagc ttcatccaat atagcatcat aatcataaga agttacctga ggcaaatcaa
     9721 tttgaccacg agcagagcaa ccattcatga aaccaaaatt tgttccacca tggaacatgt
     9781 acagattcaa ggagcccaat tcgatacatt ccataaccga atcaaccatt tcctccggct
     9841 ctctacgaat aatttcatcg ccccaacgat tgaaccaacc atcccaaaat tccatacaca
     9901 tgagtggcca attcttctga tgttcattaa agaatgccgt catatttgca aaattctctc
     9961 tagctttaga accaaagttt cctgttacaa atacatcgtc ctctatcagt gttcctgcac
    10021 gtaatgttgc tcgccaagaa ccgtctgagg taaacaaggg agcagttaga ccatgtttac
    10081 gcatcaagcc agccaccgat cttagatact ccttctcctc accataagaa ccatactcat
    10141 tttccacttg gaacataagt acatttccac cctgtgcgag ttggagcttg gccaatttag
    10201 gaataagaga agcgtaatat tcatctaagt gctgcaagta caccgaatca ctcgaacgta
    10261 ctctaagctc ttccttcatc aaccaagcag ggaggccacc ccattcccac tccgcacaaa
    10321 tataagggga aggacgtaca attgcataaa gccctaattc ttgagccaat ttcaaaaatc
    10381 gctcaatatc caatataccc tcgtaacaaa actcaccctt tcgaggttca tgcatattcc
    10441 aagggacata ggtttcaacc gtattaaaac caagggcctt taagttatac aaagaatggt
    10501 accaatcatc aggatggaca cgaaaataat gaatcgcgcc tgataaaatc ttaaagggtt
    10561 caccgtccaa ataaaattgg tctccaatat aaaattcttt catttcttcc tccgatgcaa
    10621 ccgcttacct ttctattaat atactaacac ttatttgtgg ttatgtccag tactttttaa
    10681 aaattttttt accatttctt atcaccttta taaagtgaaa aagtatattt ttgtgggtat
    10741 tttagctata ttttttgatt ttcatcaact aattgagtat aataagctgt ggaaaggaag
    10801 gaaataagta tgaaagtacc gaagtaccaa cttatacaaa acgacttacg ccaacagatt
    10861 gtttctggca aatttgaaaa tggcgacaaa ttttacacag aatctgaatt aacaaaacta
    10921 tttaatgtta gctccattac cgttatccgt gcagtaaacg aactagttaa agatggttac
    10981 ctcgttcgcc aacaaggtaa aggaaccttt gtatctcgtt cacgcaaagg acgtcttgta
    11041 gaattttctg atattgagat ttttcctatg gataaggata aggttactgt tctctcgtgc
    11101 gaaaaaggca ataaacccga tatccttgaa aaactaaatc ttgacaagaa tgagttttac
    11161 tataaaattg tgcgtgtccg cgcagcagaa gacactccat acatcttcca caattcatac
    11221 atccctcaac gatatatcca gaatcccgat gctccactcg agcactatca atcaatctat
    11281 caacgcttca aactggacta caatatccac atgtccgagg aaccatttgt agaaacgaat
    11341 gaaattgtca gcccatgtcc aaaagaggtt gccacccatt tgaaattaaa agcgactgaa
    11401 ccagctgtac tacaaaacaa aacaactact aacagtacta gcggtgaagt gatggaatat
    11461 actgaaactt acaaacactg gaagtattac aaatttgaaa ttacagccaa ccatcgctaa
    11521 aaaaacgcag actaacgtcc gcgttttttt catacccaat aacaatcaaa agtagcctag
    11581 gaaacgaagg cgaagataga actggatttc atcaaggctc ttgacaacga atagttttga
    11641 ttttcgaaga gtattgattc taatatcccc gttgatgggt aatcttgtaa cgaaactggt
    11701 ccgctctagc aatactaaac gtaaactcga taattctatt cttatcatta tacgtttttc
    11761 ttaccaaatg aagcactgga ctattgcttg gaattccgag tatcttagct tcattatcca
    11821 aagcaatact tgcatagaac tcttcttctg ccaaacgaat cacctgatga taatcctctg
    11881 aaaaaatttc atacaaggga gttgacttca ttcgctgagc agctaatcca ggaaatagcg
    11941 aggcaggaac atatgtcctt tccaacatca tcggaacctc atctgcaagt ctcaaacgct
    12001 caacttcaaa cacttcctca ccctgactaa gttgtagatg ctgcgcaata tagtccgacg
    12061 ccttaaccaa ttcaaaggat aaaatagatg tcctcggtac tttcccaatt tttttcattt
    12121 gctcagtaaa actataggcc tgagacaaat caactgcagt atctgaaatc tcagatacat
    12181 aggttccctt accatgcttc ttatagacta agccacgttt ttccaattct tgcaaagcca
    12241 aacgaactgt aatacgactg acaccataaa cctgtgtcaa ctcacgctct gaaaataact
    12301 tgtcattcgg agccatcctc tcacgaattg caacttccaa ttcatcaacc aactgcaaat
    12361 agagaggttt ttcaacagtt tgactaacca tagactcctc ctttctaacc cacataagct
    12421 tacaaatcta ttttacacca aatttcatat attaacaata tcttaggtga taaaatacca
    12481 gctcttcaag ctaaagcaag gtcacctcat ctggcaaagt aatagtctct tttccctgct
    12541 tgtccattac caagattgtt gagggataaa aaggatcaaa acgactactt agatctacag
    12601 ccagtacatt tttataacct tcctgcgaaa agaccagcgt aatcttacca cgacgatttt
    12661 tgatagaaaa acggattaca ctttttaatg gaataacccc ttttaagtag tgatcaataa
    12721 cataccagaa actatcaatc acttccccag gaagcgaggt cacaactcca aaacttgcat
    12781 agcgcccatt tgtttgttca aaggccataa gcccctcctt tacaataaga aaaaagcctg
    12841 taaaacagga ttttcttttc taactttatc tacttctcag ttctaaatag cgtttgtacc
    12901 agatattaac gtaagcagga gagaatggtc cgcgctggtt attaatccaa tcaaccagta
    12961 tcttgacatt ttccttcaaa ataaaatcaa tatcatccga atacttcatc gccttacgat
    13021 gacgctcata ttcatccaca tccagcagtc tcttttcacc atcagcaaag accttaacat
    13081 ctaggtcata atcaatgtat ttgagtgcct cattgtcgag aacatatgga ctagcaaggt
    13141 tacaataata ggaaactccg ttttcacgaa tcattgcaat gatattgaac cagtattttt
    13201 tatgaaaata aacgattgct ggttctcttg ttacccaacg cctaccatca ttttcagtca
    13261 ctaaggtatg attattgact ccaataatag cattttccgt tgtttttaat accatggtgt
    13321 cacgccaagt gcgatgaatt tcaccgtcat gcttataact ttgaattgta ataaagtcgc
    13381 cttcctttgg taatttcact ttgttaccaa ctttctacaa ttacaattta tcttgactat
    13441 tatatcatat ttttctaacc taggctaaat tttttcacag ataatcacga aggacagcct
    13501 gaatccgatc aaagtcataa ccctttcgag ctaaagcctg tatcaacctt tgcttcaatt
    13561 cgtagccttc atatttacga ctatattttc tatactgctt atccaattcc ttggcaatta
    13621 agtcatcttc attttcctca tcagccacaa gttccaactg actcaggctt tgtttggccg
    13681 tctcataaga aaaacccttc cccatcaatc cctgtacaac tttatcttgc aaagctcgaa
    13741 gaggcaattt tctttggtat ctacccacca atttctcccc aatcttccca gcaagctctg
    13801 aaaaatcttc ctctgccagc cttttatcaa tcagcggttt aggaattcct ttctgcatca
    13861 acttctgacg aatcatagct ggtcccttat ctccatttag agcattctga cggacatagc
    13921 tatcaacata attcccatcg tccagccatt tttcctcttc taataccatc acaatcttga
    13981 caatattgtt ttcttcaatt tcatattttt ttagataatc actaacttct tttttggtac
    14041 gttgcttgaa ggaaaggtaa taaagtgcta gatttttccc ataagaaaac tgagcaaaat
    14101 cgcgaagctc acgaaactcc tgctccgtga tttcctttcc cttactaagc atgaagcgaa
    14161 caattgtgtc ttccgtaata tataaactat cctgaccatc cacttcaagt aaataaagac
    14221 gtttcttttt ttcaattttt gtaattctca ttctttcatt atacaggatt tttcggtaaa
    14281 ataataatat gaatctacaa gtaaaccaac gaatcccctt aaaaatcaaa cgaatgggca
    14341 tcaacggaga aggcatcgga ttttatcaaa aaacgctagt ctttgtccct ggtgccctta
    14401 agggagaaga aattttttgt caaattacct cagtcaaacg aaatttcgtt gaagcaaaat
    14461 tgttaaaaat caacaaggaa tcaaaattcc gtgtcaagcc ggcctgtgac atctatcaag
    14521 cctgtggtgg ttgtcaaatc atgcacctcc gctatgataa acaattagac ttcaaacaag
    14581 atctcttgca gcaggcccta aaaaagttca aaccagctgg ctacgaaaac tacactattt
    14641 atccaacaat cggtatggat aagcctagcc actatcgtgc caaattacag ttccagactc
    14701 gatctttcaa agatcaagtc aaggccggtc tctatgcgga aaattcccat cgcttgattg
    14761 gaattacgaa ttgttacgtc caagatgccg aaacacaaga gattatcaac ggaatcgcaa
    14821 agctactcac caagcaccgt attcctatct ataatgagcg caagcaacac ggaatccgca
    14881 cggttatggt ccgcaaggct cgaaaaacag gtcaagttca agtgatcttt atcacctctt
    14941 gtcaggttaa tcttattaaa ttaattgatg aactcaccag tcaatttcca agtataatca
    15001 ctattgctct aaactggaat agacagaaaa cgagtgatat ttatggtgaa aaaacagaaa
    15061 ttttatgggg agaacaagct atcgatgaag ctgttttaga ttacgaattc tcactatctc
    15121 cacgggcatt ttaccaatta aatccccaac aaacagaaat tctctatggc gaggccgtca
    15181 aggctttgga tatttcagga gatgaagact tgattgatgc ctactgtggc gtgggcacaa
    15241 tcggcttcgc ctttgctaaa aaggtcaaat ctgttcgagg tatggacatt attccagaag
    15301 ccattgagga tgctaaagca aacgctaagc gaatgggact atccaatacc cactatgagg
    15361 caggtaaagc agaagaaatc attccaaagt ggtaccaaga aggctacagg gccgatgcct
    15421 tggtagtcga tcctccacga actggactcg atgaaaagct cctcaaaacc attttggact
    15481 acaaaccagc taaaatggtt tatgtatcct gcaacgtctc cactcttgca agggaccttg
    15541 ttgagctaag caaagcctac aaggtagaat acattcaatc tgtcgacatg tttcctcaca
    15601 cagcacggac cgaggcggtt gttaaactaa gcaaaaaata aaaggcctat aagactatga
    15661 cgggtaaacc cactatggtc atatagagcc tttttgagtg tagaaaaaag tcccctacga
    15721 cctacactga tacccattta aaatcaaaaa tagccaccta ccactcaaag tagatatgga
    15781 gcattttgat tttgttattt tttgagaaaa aaacagctac ttgaaatcca caatcctcaa
    15841 ctcaaactta taaattaagg ttttacatgt tataagtaaa ttagacttgc tatttttgat
    15901 ttttattgag tatgaaaaac aacaaaactc acaatttcca acaccaaatc agaaacaact
    15961 aacaatctca tcaaagtcat taaaagaaat gcctttggat tcaggaaaat gaaaactaga
    16021 aaaacggatt ttgatgcttt gaacagtgaa aagctgagga gagctctttt cagctcatct
    16081 cctaaaaaca agaaagagag aacgaatttc gtcctctcta ggtgttagct tttcatctac
    16141 ccactacagt tgacaaagag ccaaaaaaaa agaacattgc tactctctta c
//
LOCUS       DAZQCJ010000039        16048 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000039 DAZQCJ010000000
VERSION     DAZQCJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16048
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.039"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(228..491)
                     /locus_tag="ACHU66_001140"
                     /pseudo
     CDS             complement(228..491)
                     /locus_tag="ACHU66_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            800..1081
                     /locus_tag="ACHU66_001141"
                     /pseudo
     CDS             800..1081
                     /locus_tag="ACHU66_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1612..2250)
                     /locus_tag="ACHU66_001142"
     CDS             complement(1612..2250)
                     /locus_tag="ACHU66_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFR3389431.1"
                     /translation="MIIHKSVNPVAFQNTYYIENDTHLIIIDPGSDWTKIQVTIEKIG
                     KPISAILLTHTHYDHIMSLDILRETYNFPPVYVAESEASWLYTPEMNLSGLPRHDDMK
                     NVVCRPAEKIFQYQEEYQFDGFHFKVVPTPGHSIGGVSFIFPEDECVITGDALFRETV
                     GRTDLPTSNFDDLITGIREHIFTLPNHYTVHPGHGQNTSVGHEKNFNPFFRM"
     gene            2367..4826
                     /locus_tag="ACHU66_001143"
     CDS             2367..4826
                     /locus_tag="ACHU66_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HFR3389432.1"
                     /translation="MKDTRTSNRYAVVDLEATGTGTDAKIIQIGIVLVENGEIIDSYA
                     TDINPYELLDDHIKNLTGITDQQLSQAPDFGQVASTIYDMIGDAIFVAHNVKFDANLL
                     AEALFFEGYELLTPRVDTVELSQLFFPTFEKYSLGNLAEHLELGLDQAHTAISDAMAT
                     ARLLIKIQEKIKSLPRSIVEKILDLADNLLFESRMVIDEMVPVLSENLSYDLESIHDL
                     VLKKPEKIKSAYRLSEDFSTNIALLGLHERKKQTAFAQVVEKRLVDEEKVHFIQAQAG
                     LGKTYGYLLPLLAKSDQPLLVTVPTKLLQEQIMEKEGSKLKEIFRIPIVSLKSPKHFI
                     KLDNYWKTLQRQDDNRLVNQFKMQVLVWLCETTTGDLDELKQKQRYQAYFDEIAHDGK
                     LEPESLFWDWDFWQRLNQQALSSRLLITNHAYFLNHLKDQDPLMDKRLVVIDEAQKFL
                     LAAENLATASQDLTSMLQVLQSKKDRATTILDQRLYESCQFELNHFLSRFRQHGQREV
                     QKAELHQLGQNLEELGDVDLNDLHQLIDYYDTFWLEEKNLEEKRLAYLRGSKDSLLNA
                     TNYLPDTKIFCISATLTISKKVSLADLLGFEQVTLDLIPTQTVTNQQLLFPTHLPDIL
                     SWTKEEHAAYLATTLGEIAELGRPILVLFTSISLLLQVSELLEDNNIPHLAQHKHGQE
                     MPLKRKFERGECQILLGTGVFWEGVDFASQNQLIQVITRLPFDNPKDRFVQKINHHLR
                     EEGKNPFYDYSLPMMMIKLKQAIGRTNRHDKQDSLVLVLDPRVHTKRYGQQILSFFEK
                     DYSMLSVDAKEIEGAIQDFFE"
     gene            5363..6439
                     /gene="proB"
                     /locus_tag="ACHU66_001144"
     CDS             5363..6439
                     /gene="proB"
                     /locus_tag="ACHU66_001144"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR3389433.1"
                     /translation="MTEKTIVFKVGTSSLTQENGSLDRIKIARITNQLAQLHQKGYQI
                     VLVTSGSIAAGFRRLGFDKRPTKIAEKQASAAVGQGLLIEEYTQNLMKDGIVSAQILL
                     TQDDFADARRYQNASQALQVLLKQRAIPIINENDTIAIEEIKVGDNDTLSAQVASLLK
                     ADLLVLLTDVDGLYTANPNSDPTAQHLPEIKEITEDLFAMAAGAGSSNGTGGMTTKLQ
                     AAQIATKSGVPVFICSSKEDTALLQAVTQANRGTLFLADDHAMNQRKQWMAFYARTDA
                     AVEVDAGAVDAMLHQGRSLLATGVKALEGDFEVGQVVEVYSQADHRLIGKGRVKLSSK
                     DLQDQLANGRAEGVLIHRNDWVSL"
     gene            6449..7687
                     /locus_tag="ACHU66_001145"
     CDS             6449..7687
                     /locus_tag="ACHU66_001145"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR3389434.1"
                     /translation="MTTTQALLDSLLAHKTSINLATTEQKNQALSAMADQLVAQTEAI
                     LAGNTIDMENAQGKISQVMQDRLLLTAERVEAMAEGIRALIGLPDPVGLVLEESTRAD
                     GLNIRKKSIPFGLAGMIYESRPNVTSDAAALAIKSGNAVILRGGKEAFHSAQAIVTAL
                     KTGLEQAGLSPKVIELVQDTSRASATELMTAKGKIDLLVPRGGAGLIQAVVENATVPV
                     IETGTGICHVYVDKDADLDKAVQIVVNAKTSRPSVCNAAEVLLVHEAIAGQFLPRLEE
                     ALAGQVELRADSQAQAILNQATPAGDQDFDTEFLDYIMAVKVVSNVEEAISHIAQHST
                     GHSEAIVTENSQTAELFTLHVDSAAVYVNASTRFTDGGEFGLGCELGISTQKMHARGP
                     MGLREMTTYKYIITGDGHIR"
     gene            7697..8482
                     /gene="proC"
                     /locus_tag="ACHU66_001146"
     CDS             7697..8482
                     /gene="proC"
                     /locus_tag="ACHU66_001146"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR3389435.1"
                     /translation="MKIGFIGLGNMGAALAHAVSQQPDTQLLLSNHNPAKAQALQTQL
                     GGQLLSNGQIAEQAEVIFLGVKPHLIQTVLSGLQDQISQNPSTIWISMAAGVTLDSLV
                     TFVSADKIIRIMPNTPVAIGQGMTTYSLVNQELAPLLEQILEKSGKVQQVPESLIDAA
                     TAIAGCGPAFVYQMIEALTDAGVQNGLTAQDAKVLAAQTLAGTAQMVLTSDKHPAQLR
                     QEVTSPGGSTIAGVVALEKEGFRYAIIKAVTAALKKTRKLGKK"
     gene            complement(8505..8909)
                     /locus_tag="ACHU66_001147"
     CDS             complement(8505..8909)
                     /locus_tag="ACHU66_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR3389436.1"
                     /translation="MAGRKISPQSLKNLYQSNKEANQLTKESIETALLFLLEKKELKQ
                     ISVSELVRKAGVSRNAFYRNYKSKEEILEIYYERTSSNLKKKWHDLQDKVQKDGVKQS
                     FADFVHEQKRKAEQSKALSNVSQWIKEKTKRD"
     gene            9036..9887
                     /locus_tag="ACHU66_001148"
     CDS             9036..9887
                     /locus_tag="ACHU66_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFR3389437.1"
                     /translation="MSKWKIVADSGCDYRQLANLAPDTEFISVPLTIQVGEQAFVDDA
                     SLDIDHMMEVMEASKAAAGSACPSPQAYQAAFEGAENIIVVTITGGLSGSFNAARVAR
                     DMYIEEHPNVNIHLIDSLSASGEMDLLVDEINRLIGSGLDFPQVVEAITHYREHSKLL
                     FVLAKVDNLVKNGRLSKLVGTVVGLLNIRMVGEASAEGKLELLQKARGHKKSVTAAFE
                     EMKKAGYDGGRIVMAHRNNAKFFQQFSELVKASFPTAVIDEVATSGLCSFYAEEGGLL
                     MGYEVKA"
     gene            complement(9986..11245)
                     /locus_tag="ACHU66_001149"
     CDS             complement(9986..11245)
                     /locus_tag="ACHU66_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFR3389438.1"
                     /translation="MKKYQVIIQDILTGIEEHRFKRGEKLPSIRQLREQYHCSKDTVQ
                     KAMLELKYQNKIYAVEKSGYYILEDRDFQDHTVELNPADFQELPYEDFRICLNESLIG
                     RENYLFNYYHQQEGLAELISSVQSLLMDYHVYTKKDQLVITAGSQQALYILTQMETLA
                     GKTEILIENPTYSRMIELIRHQGIPYQTIERDLDGIDLEELESIFQTGKIKFFYTIPR
                     LHNPLGSTYDIATKTAIVKLAKQYDVYIIEDDYLADFDSSHSLPLHYLDTDNRVIYIK
                     SFTPTLFPALRIGAISLPNQLRDTFIKHKSLIDYDTNLIMQKALSLYIDNGMFARNTQ
                     HLHHIYHAQWNKIKDCLEKYALNIPYRISKGSVTFQLSKGILSPSIQHMFEKCYYFSG
                     QKADFLQIFFEQDFADKLEQFVRYLNE"
     gene            11367..12101
                     /gene="yaaA"
                     /locus_tag="ACHU66_001150"
     CDS             11367..12101
                     /gene="yaaA"
                     /locus_tag="ACHU66_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR3389439.1"
                     /translation="MKIIIPNAKEVNTNLENASFYLLSDRSKPVLDAISQFDVKKMAA
                     FYKLNEAKAELEADRWYRIRTGQAKTYPAWQLYDGLMYRYMDRRGIDSKEENYLRDHV
                     RVATALYGLIHPFEFISPHRLDFQGSLKIGNQSLKQYWRPYYDQEVGDDELILSLASS
                     EFEQVFSPQIQKRLVKILFMEEKAGQLKVHSTISKKGRGRLLSWLAKNNIQELSDIQD
                     FKVDGFEYCTSESTANQLTFIRSIKM"
     gene            12206..13651
                     /locus_tag="ACHU66_001151"
     CDS             12206..13651
                     /locus_tag="ACHU66_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFR3389440.1"
                     /translation="MKKRSGRSKSSKFKLVNFALLGLYSITLCLFLVTMYRYNILDFR
                     YLNYIVTLLLVGVAVLAGLLMWRKKARIFPALLLVFSLVITSVGIYGMQEVVKFSTRL
                     NSNSTFSEYEMSILVPANSDITDVRQLTSILAPAEYDQDNITALLDDISKMESTQLAT
                     SPATSYLTAYQSMLNGESQAMVFNGVFTNILENEDPGFSSKVKKIYSFKVTQTVETAT
                     KQVSGDSFNIYISGIDAYGPISTVSRSDVNIIMTVNRATHKILLTTTPRDSYVAIADG
                     GQNQYDKLTHAGIYGVNASVHTLENLYGIDISNYVRLNFISFLQLIDLVGGIDVYNDQ
                     EFTSLHGNYHFPVGQVHLNSDQALGFVRERYSLTGGDNDRGKNQEKVIAALIKKMSTP
                     ENLKNYQAILSGLEGSIQTDLSLETIMSLVNTQLESGTQFTVESQALTGTGRSDLSSY
                     AMPGSQLYMMEINQDSLEQSKAAIQSVLVEK"
     gene            13669..14358
                     /locus_tag="ACHU66_001152"
     CDS             13669..14358
                     /locus_tag="ACHU66_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFR3389441.1"
                     /translation="MNNQEVNAIEIDVLFLLKTIWRKKFLILLTAVLTAGLAFVYSSF
                     LVTPQYDSTTRIYVVSQNVEAGAGLTNQELQAGTYLAKDYREIILSQDVLTQVATELN
                     LKESLKEKISVSIPVDTRIVSISVRDADPNEAARIANSLRTFAVQKVVEVTKVSDVTT
                     LEEAVPAEEPTTPNTKRNILLGLLAGGILATGLVLVMEVLDDRVKRPQDIEEVMGLTL
                     LGIVPDSKKLK"
     gene            14368..15045
                     /locus_tag="ACHU66_001153"
     CDS             14368..15045
                     /locus_tag="ACHU66_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFR3389442.1"
                     /translation="MAMLEIARTKREGINKTEEYFNAIRTNIQLSGADIKVVGITSVK
                     SNEGKSTTAASLAIAYARSGYKTVLVDADIRNSVMPGFFKPITKITGLTDYLAGTTDL
                     SQGLCDTDIPNLTVIESGKVSPNPTALLQSKNFENLLATLRRYYDYVIVDCPPLGLVI
                     DAAIIAQKCDAMVAVVEAGNVKCSSLKKVKEQLEQTGTPFLGVILNKYDITTEKYSEY
                     GNYGKKA"
     gene            15084..15815
                     /gene="cps4B"
                     /locus_tag="ACHU66_001154"
     CDS             15084..15815
                     /gene="cps4B"
                     /locus_tag="ACHU66_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HFR3389443.1"
                     /translation="MIDIHSHIIFGVDDGPKTIEESLSLISEAYRQGVRYIVATSHRR
                     KGMFETPEKIIMINFLQLKEAVAEVYPEIRLCYGAELYYSKDILSKLEKKKVPTLNGS
                     RYILLEFSTNTPWKEIQEAVNEMTLLGLTPVLAHIERYDALAFQSERVEELIDKGCYT
                     QVNSNHVLKPALIGERAKEFKKRTRYFLEQDLVHCVASDMHNLYSRPPFMREAYQLVK
                     KEYGEDRAKALFKKNPLLILKNQVQ"
     gene            15840..>16048
                     /locus_tag="ACHU66_001155"
     CDS             15840..>16048
                     /locus_tag="ACHU66_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFR3389444.1"
                     /translation="MNIEIGYRQTKLALFDMIAVAISAILTSHIPNADLNRSGIFIIM
                     MVHYFAFFISRMPVEFEYRGNLIEF"
BASE COUNT         4764 a         3064 c         3616 g         4604 t
ORIGIN      
        1 gtgtcttgcc agtttttgat gcgacgtcta gcgtcaaaaa gccccacgac ttaacaagac
       61 acctctactt taacataaag aaaaagtagt aagtactctc tcccgtcgga gatttcctca
      121 ctactaatct tagtatgttt tcgtctggtc gagttcttat acgaaaactc aaaagaatgc
      181 aaaaattccc ttcctatgat ataatcaaag cgaaaatatt ttttatgtta ggctactagt
      241 tcgtccctag tagtcttttt ctgtgctaaa aattcaggat acgttatctg ctctttcaag
      301 aggatataag ctatttttaa taattgatgt gcaagggcaa ttgttgcttt ttgtgaacca
      361 cgccgacttt gaatctgata aaatcgttca gctaaaggac ttcctttttg tcttttgata
      421 gcgaaagcgg cttggcataa acatttcttc aaataggaat tcccgtttcg aatcttggta
      481 cttcgttttt cccactccct aggaactttt tattatatga cttttagtcc atcacaatcc
      541 gtaaagtatg tgacaaatga accgaggatg ggcaaaaaat agtcctataa tttctgtagc
      601 gggtaaaacc actatggagc cttttaaatg taaaagccat atgacctata atgaaaagtg
      661 acaaaactta cattagaaag aggtcatatg gaataaatca agagttaggg gagtgactct
      721 tgattcctga gcctagaaat agaaaagcga agatcttatc acaaattttc ttggaataga
      781 agacgaaaat atcgctatca tgactgaata tgatgtggaa actcacttag attgtgtgtc
      841 tccaaaatgt cctgcctgaa agggacaaat agtgaaatac gacttccaaa gagcatctaa
      901 aatcctttgc ttagaagcag ctgataatcc tttctcctta tccatcttag aaagcgtcgg
      961 ttcaagtgta aaggatgcgg gaaaatggtt gtcgctgaaa ttcacttatt aaaaaaacga
     1021 ctaaatctct gttgctatta accagaaaaa accccaatta ctactcgttg agaatcaagc
     1081 aaagagtaag caatattcac ctgatactat tgttgtcaag gatagctttt ctatcctatt
     1141 cggaactata aagtaagagt ttttgaaggg ggcagatatt ggttgcaatg tattgaaaaa
     1201 cttgtggttc catggttttt aaggttactc tatctggcta tattttgaac tatgttatta
     1261 caccagtttc tgattatgta gtgaattgct tgttggaagg atatcagcaa tctgtgattt
     1321 tagtagattt agtcaggagt ttaaagacaa cttggaacag aaggatagaa catggaagga
     1381 gctaattaat ataacaaata gagattatta gccatgaggc gaaccattga aactcgtttt
     1441 ctgaatcata cgctttgttt gatacagaac aaacctttgc cagaggactg ctcggattac
     1501 agctacggat tgcacaaatc ctactgactt ataatctaag ctattttgac tttaactagt
     1561 agcacgggta aaaaaactgc ctagttcaat cagaactggg cagtacttat tttacattct
     1621 aaaaaatgga ttgaaatttt tctcatgtcc aacgctggta ttttgaccat gacctggatg
     1681 gacagtatag tgatttggca gggtaaagat atgttctctg atgccagtaa tcaggtcatc
     1741 aaagttgctg gttggcaggt ctgtgcgtcc cacagtttca cggaaaagtg cgtctccagt
     1801 gatgacacat tcatcctctg ggaagataaa ggaaacacca ccaatagagt gcccaggtgt
     1861 tggaactacc ttgaaatgaa aaccgtcaaa ctggtattct tcttggtatt ggaagatttt
     1921 ttcagcaggt cggcagacta catttttcat atcatcatga cgaggtaggc ctgatagatt
     1981 catctctggt gtgtagagcc agctggcttc actttcagcc acatagacag gcgggaaatt
     2041 gtaggtctca cgcagaatgt ccaaactcat aatatggtca tagtgagtgt gagtcaggag
     2101 aatagccgaa attggttttc cgattttttc gatagttact tgaatttttg tccagtcact
     2161 cccagggtca atgataatca gatgggtatc attttcaatg tagtaggtat tttgaaaagc
     2221 gacaggattg acagatttgt gaataatcat tctttttcct tcttctaaca gttactgatg
     2281 ttactagtct aacaaaaaaa cacgccttct tcatggaatt tgtttggagc tttttccatg
     2341 agagggaata tgctataatg aacagtatga aagacacaag aacaagcaat cggtatgccg
     2401 ttgtagattt ggaagcaact ggtacaggta ccgatgcaaa aatcattcaa atcgggattg
     2461 ttctcgttga aaatggagaa atcattgaca gttacgcaac ggacatcaat ccctatgagc
     2521 tactggatga ccatatcaag aatctgacag gtatcacaga ccaacagcta agtcaggctc
     2581 cagattttgg tcaggtagca agtaccattt acgacatgat tggagatgct atttttgtcg
     2641 cccataatgt caagtttgat gctaatctct tggcagaagc actctttttt gaaggctatg
     2701 aactgttgac accgcgtgta gatacggtag aattatcaca gctatttttt ccgacttttg
     2761 aaaaatatag cttgggaaac cttgcagagc atttggaact tggtttggac caggcacaca
     2821 cggccatatc agatgccatg gctacggcgc gcttgctcat caaaattcaa gagaaaataa
     2881 agagtttacc caggtctatt gttgaaaaga ttcttgattt ggcggacaat ctgctctttg
     2941 aatcccggat ggtcattgat gagatggtgc ctgttctgtc agaaaatcta tcatacgatt
     3001 tagaatctat tcatgacttg gtcttgaaaa agccagaaaa aatcaagtca gcctaccgat
     3061 tatctgaaga tttttcgacc aatattgccc ttctcggctt gcatgaacga aaaaaacaaa
     3121 ccgcttttgc tcaagtagta gaaaaacgct tggttgacga agaaaaagtg cattttatcc
     3181 aagcgcaggc aggtttgggg aagacttacg gttatctctt gcctttactt gctaagtcag
     3241 accagccttt attggtcacc gtaccgacca agctccttca agagcagatt atggaaaagg
     3301 aagggagcaa gcttaaagag atatttcgta tccccatagt ctctctaaaa tcgcccaagc
     3361 attttatcaa gttagacaac tactggaaaa ccctgcagag gcaggatgat aatcgcctgg
     3421 tcaatcaatt caaaatgcag gtgcttgtct ggttatgcga aaccacgaca ggtgatttgg
     3481 atgagcttaa gcaaaaacaa cgctatcaag cttactttga tgagattgcc cacgatggga
     3541 aattggagcc tgaaagtctt ttttgggact gggatttttg gcaacgattg aaccaacaag
     3601 ctctctctag tagactgctc attaccaacc acgcttattt tctcaaccat cttaaggatc
     3661 aagaccctct catggataag cgtctggtgg tgattgatga agcacaaaag tttctcttgg
     3721 ctgctgaaaa tctagcgacg gcttcgcaag atttgacaag tatgttacaa gtattgcaaa
     3781 gtaaaaagga cagagcgacg actattttgg accaacgttt gtatgagtct tgccagtttg
     3841 aactcaacca ctttttgagt cgttttcgtc agcatggtca acgagaggtt caaaaggctg
     3901 aacttcatca acttggtcag aacctggagg agcttggaga tgttgatttg aacgacttgc
     3961 atcagctcat agactattac gatacctttt ggttggagga gaaaaattta gaagaaaagc
     4021 ggcttgccta tttgcggggc tccaaggaca gcctcttaaa tgcgaccaac tacctgccag
     4081 atactaagat tttctgcatc agcgccacct tgacaatcag caaaaaggtc agtctggcgg
     4141 atttgctggg ctttgagcag gtcaccttgg atttgatacc gactcaaact gtgaccaacc
     4201 agcagctgct ttttccgacc catctccccg acatcctctc ttggaccaag gaggagcatg
     4261 cagcttattt ggctactact ttaggagaaa tagcagagct gggacgaccg attttggtct
     4321 tgttcacatc gatttctctg ctcttgcagg tgtcggaatt gttagaagac aataacattc
     4381 cccacctagc ccagcacaag catggacagg aaatgcctct caaacgcaaa tttgaacgag
     4441 gggaatgcca aattttactc ggcacaggtg tattctggga gggcgttgac tttgccagtc
     4501 agaatcaatt gattcaggtt attacaaggc tcccatttga caatccaaag gaccggtttg
     4561 tacaaaaaat caatcaccat ctgcgtgaag aagggaaaaa tccattttat gattacagcc
     4621 tgcctatgat gatgattaag ctcaaacaag ccatcggtcg taccaatcgc catgacaaac
     4681 aagattcact agtcttggtt ttggacccgc gtgttcacac caaacgctat ggacagcaaa
     4741 ttctgtcctt ctttgagaaa gattattcaa tgttaagtgt ggatgcgaaa gaaatagaag
     4801 gagctattca agattttttt gaataagttg aaagggagtg agaaagaact cgactggtct
     4861 aaaaagagtt cgtcaccaag tctttatttc tagttgttga gctgaaacag tctatcccca
     4921 gactgtttca ctcccacccc cgcatagttc caactgtctg ggggacagtt ggaagttgga
     4981 aatagagcga acaaagtttg ctacaaaact gtctgggaga cagtttgagg ttggagatga
     5041 ggcgaactct gttcgcatca gtcgtatagg tcagatttgg agtgtaatac acgaattgct
     5101 gagatttgct tcgcaaatct tatctccaac ctttaacagt ccactggact gttaaaccca
     5161 atcaaccact gcgctgagat gttgacacga actctgagta gtagccgctg ggctttttgc
     5221 ccagcctctt tgtttccagc gttttttaat gctactcatg attaggaatc tattccctct
     5281 ctccttgccg aataacagtg attttcttta tgattaaaat atggtataat tttccaaata
     5341 ttagtttttg tgagaagaga ttatgacaga aaaaacaatt gtatttaagg tcgggacgag
     5401 ttctctgacg caagaaaatg ggagtttgga ccgtatcaaa atcgctcgga ttaccaatca
     5461 gctggctcag ttgcatcaga agggctacca gattgtgctg gtgacgtcgg gctcgatcgc
     5521 tgctggtttt cggagattgg gctttgacaa gcggccgact aagattgcgg agaagcaggc
     5581 ttctgcggct gttgggcaag gtttgctgat tgaggaatac acgcaaaatc taatgaaaga
     5641 cggcatagta tcggctcaga ttttgttgac ccaggatgat tttgcggatg ccagacgtta
     5701 tcagaatgct tctcaggcct tgcaggtctt gctcaagcaa cgagctattc ctattatcaa
     5761 cgaaaatgat accattgcta ttgaggagat taaggtgggg gataacgata ctttatctgc
     5821 ccaggtggct tctctcttga aagcagatct cttggtgctt ttgacggatg tggatggact
     5881 ttacacggcc aatcccaaca gcgatccgac tgctcagcac ttgcctgaaa tcaaggaaat
     5941 tacggaagat ttgtttgcca tggcagcagg agcaggctcg tctaacggaa caggtggcat
     6001 gaccaccaaa ttgcaggcgg cccagattgc gaccaagtca ggcgtacccg tctttatctg
     6061 ctcttcaaaa gaggatactg ctctcttgca ggcagtcacc caggccaatc gtggcaccct
     6121 ctttttagca gatgaccatg ccatgaacca acgcaagcag tggatggcct tctacgctcg
     6181 gacagatgcg gcggttgaag tggatgcagg agcagttgat gccatgttgc accagggacg
     6241 cagtttgtta gctactggtg tcaaagccct cgaaggtgac tttgaagtgg gtcaggtcgt
     6301 ggaagtttac agccaggcag accaccgttt gattggtaaa gggcgggtca aactgtcctc
     6361 caaagacttg caggaccagt tggcaaatgg tagagcagag ggtgtgctca ttcaccgcaa
     6421 cgattgggtg agcttatagg agaaaaatat gacaacaaca caagcattat tagatagttt
     6481 attggctcat aagacctcta tcaacttagc gacaaccgaa cagaaaaacc aggccttgtc
     6541 agctatggca gaccaattgg tcgctcaaac ggaggcaatt ttagcaggca atactattga
     6601 tatggagaat gcccaaggaa agattagcca ggtcatgcag gaccgtttgc tcttgacagc
     6661 tgagcgggtt gaagctatgg cagagggcat tcgtgccctg attggcttgc cagatcctgt
     6721 tgggctagtc ttggaagaat ccactcgggc agacggcttg aacattcgca agaaatccat
     6781 tccttttggc ttggcaggaa tgatttacga aagtcgtccc aatgtgactt cagacgccgc
     6841 tgctttggca atcaagagcg gaaatgcagt catcttgcgt ggtggcaagg aagcctttca
     6901 ttcggctcag gctattgtca cagccctcaa gactggtttg gagcaggctg gtctttctcc
     6961 aaaggtcatc gagttggtcc aagataccag ccgggcctct gcgacagagt tgatgactgc
     7021 caaaggcaag attgacctgt tggtgccacg tggcggtgca gggctaattc aagctgttgt
     7081 agaaaatgcg actgtgccag ttatcgaaac aggcacaggc atctgccatg tctatgtgga
     7141 caaggatgcg gatttggata aggctgttca gattgtggtc aacgccaaaa ccagtcgtcc
     7201 ctcagtctgt aatgcagcgg aagttttgct agttcacgaa gctattgctg gtcaattcct
     7261 gcctcgcttg gaagaagctc tggctggtca ggtggaatta cgtgccgaca gtcaggccca
     7321 ggctattcta aaccaagcaa caccagcagg cgaccaagat tttgacacgg aatttttaga
     7381 ctatatcatg gctgtcaaag tcgtttcaaa cgtagaagag gctatcagtc atatcgctca
     7441 acactcgaca gggcacagcg aggccatcgt gacggaaaac agtcaaacgg cagaactttt
     7501 cacactccat gtggactcgg ctgcggtcta tgtcaatgcc tcgacccgtt tcacagacgg
     7561 aggcgagttc ggtctgggct gcgagttggg catttccacc cagaagatgc acgctcgtgg
     7621 tcctatgggt ctgcgtgaga tgaccaccta caagtacatc atcacaggcg acggccacat
     7681 tcgttaggag gacaagatga agattggatt tatcggactg ggcaacatgg gagcggcttt
     7741 ggctcatgca gtcagccagc agccagatac ccagctcctc cttagcaacc ataacccagc
     7801 aaaagcccag gctctacaaa ctcagctggg cggtcagcta ttgtctaatg gacaaatagc
     7861 agagcaggcc gaggtgattt tccttggggt caagcctcac ctgattcaga cagtcttgtc
     7921 aggcttgcag gaccagatta gccagaatcc gtcaactatc tggatttcca tggcagcggg
     7981 tgtgaccctt gacagcctgg taacttttgt atcggcagac aagatcattc gtatcatgcc
     8041 caacacacca gtcgctatcg gccaaggcat gacaacctat agcttggtca atcaagagct
     8101 tgctccgcta ttggaacaaa tcctagaaaa atcaggcaag gtccagcaag tgccagagag
     8161 cttgatcgac gcagcaacgg ctatcgcagg ctgcggacca gcctttgtct atcagatgat
     8221 tgaggctttg accgatgctg gtgtgcaaaa cggactgaca gctcaagatg ccaaggtttt
     8281 ggcggcccaa accctagcag gaactgctca gatggtctta accagcgata aacacccagc
     8341 ccagctgaga caggaagtga cctcgccagg cggctcgact attgcaggcg tggtagctct
     8401 tgaaaaagaa ggcttccgtt acgccattat caaggcagtc accgccgccc tcaaaaagac
     8461 tagaaaatta ggcaaaaaat agaagacagc aaaaaacggg acttttaatc ccgttttgtt
     8521 ttttccttga tccattgact gacattggac agggccttgc tttgctctgc cttgcgtttt
     8581 tgttcgtgga caaaatctgc aaagctttgc ttgacgccat ccttttgaac cttgtcctgc
     8641 aaatcatgcc atttcttttt aaggttgctg gaagtccgtt cgtaataaat ctctaaaatt
     8701 tcttcttttg acttataatt gcgatagaaa gcgttacgag aaactcccgc tttacgaacc
     8761 aattcagata ccgaaatttg cttgagctcc tttttctcta ggagaaatag cagagcagtt
     8821 tctattgatt ccttagtcag ttgattcgct tctttatttg attgatagag gtttttcaaa
     8881 gattggggtg aaattttacg acctgccata gttttccttt tccgagtgtc acatctgaaa
     8941 gaaaatgctt tcagatgggg tagtttaatg atataattgt aagtaaatag agatttattg
     9001 tcaaataaaa aagtaaaatt ggaaatgaga atattatgtc aaaatggaaa attgttgcag
     9061 attcgggctg tgactatcgt caattagcta atctagctcc ggatacagaa tttattagtg
     9121 tgccacttac cattcaagtt ggagaacaag catttgttga tgatgcaagc ttggatattg
     9181 atcacatgat ggaggtgatg gaggcatcta aggctgcggc tgggtcggcg tgtccaagtc
     9241 cgcaggctta tcaggcagct tttgagggag ctgagaacat tatcgttgtg acgattacag
     9301 gtgggctatc gggtagtttt aatgcggcac gtgtagctag ggatatgtat atcgaagagc
     9361 atccgaatgt caatatccat ttgatagata gtttgtcagc cagtggggag atggatttac
     9421 ttgtagacga aatcaatcgc ttaattggtt caggattaga ttttccacaa gtagtagaag
     9481 cgataactca ctatcgggaa cacagtaagc ttctctttgt tttagcgaag gttgataatc
     9541 ttgttaagaa tggaaggctg agcaaattgg taggcactgt cgttggtctt ctcaatatcc
     9601 gtatggttgg tgaggcaagt gctgaaggaa aattagagtt gcttcaaaag gcgcgtggtc
     9661 ataagaaatc tgtgacagca gcctttgaag aaatgaaaaa agcaggttat gatggtggtc
     9721 gaattgtcat ggcccaccgc aacaatgcta agttcttcca acaattctca gagttggtaa
     9781 aagcaagttt tccaacggct gttattgacg aagttgcaac atcaggtcta tgcagttttt
     9841 atgctgaaga aggtggactt ttgatgggct atgaagtgaa agcgtgattc acagagtaac
     9901 aattttggga tgtaatttcc actatagaat aatccccctc gtcttctaag ttcgaggggg
     9961 attgtttgta tgagactatt ggatttcatt cattcaaata tcttacgaat tgctccagtt
    10021 tatctgcaaa atcttgttca aagaagatct gtaagaaatc agctttctgt ccgctgaaat
    10081 aataacactt ttcaaacatg tgttggatgc taggcgaaag aatccccttg cttagctgaa
    10141 aggtcacgct ccctttggaa attcgatacg ggatgttcaa agcgtatttc tctaaacaat
    10201 cttttatttt attccactga gcgtgataaa tgtgatgaag atgctgtgtg ttccgtgcaa
    10261 acataccgtt atcaatgtag agtgagagag ctttttgcat gataagattg gtatcgtagt
    10321 cgattagact cttatgtttg atgaaggtat cacgtagctg gttaggaagg ctgattgcac
    10381 cgattcggag ggcaggaaag agtgtcggtg taaaagattt tatatagatg acgcgattat
    10441 ctgtatcaag atagtgtaaa ggtaggctat gactagagtc gaaatccgcc aaatagtcat
    10501 cctcaatgat gtagacatcg tattgctttg ctaattttac gatggctgtt tttgttgcta
    10561 tatcataggt tgaaccgaga gggttgtgca agcgaggaat tgtgtagaaa aacttaattt
    10621 ttccagtttg gaagatactt tccaattctt ctaggtcaat tccatctaaa tcccgttcaa
    10681 ttgtttgata ggggattcct tgatgtcgaa tgagctctat cattcgtgaa taggtagggt
    10741 tctctatcaa gatttccgtt tttccagcca aggtttccat ttgtgtgaga atatatagag
    10801 cttgttgact accagctgtg ataaccagtt ggtctttttt tgtatagaca tgatagtcca
    10861 ttaacagact ttgaacggag gaaatcaatt ctgccaatcc ctcttgctgg tgatagtagt
    10921 tgaataggta attttcccgg ccaataagac tttcgtttag acaaatccga aaatcttcat
    10981 agggtaattc ttgaaagtct gcaggattga gctctacagt atggtcttgg aaatctctat
    11041 cctctaagat ataataaccg cttttttcga cagcgtagat cttattttgg tattttaatt
    11101 ccaacatagc cttttggaca gtgtctttgc tacaatgata ttgctcgcgg agttgacgga
    11161 tagaaggtaa tttctctcca cgtttgaatc gatgttcctc tattccagtc aaaatatctt
    11221 ggatgataac ttgatatttt ttcatctagg tccccttttt atagactatg ttactagcta
    11281 gtatatagaa aaaattgaag aaagacaata tatgaataat ggggttgagg ttcaggaatt
    11341 aagctactct atggtataat taagtgatga aaataattat acctaatgca aaagaagtaa
    11401 atacaaatct agagaatgcc tcgttttatc tcctgtctga tcgaagcaag ccggtgctgg
    11461 atgccataag tcaatttgat gtaaaaaaga tggctgcctt ttataaattg aatgaagcaa
    11521 aggctgagtt agaagctgac cgttggtatc gaatcaggac aggtcaagca aaaacctatc
    11581 cagcctggca gttatatgat ggtctcatgt atcgttatat ggataggcga ggtatagatt
    11641 cgaaagaaga aaattatcta cgtgaccacg ttcgtgtagc gacagcctta tacggattga
    11701 ttcatccttt tgaattcatt tcacctcacc gcttagattt tcaagggagc ttaaagatag
    11761 gcaatcagtc tttgaaacag tactggcgac cgtattatga ccaagaagtt ggtgatgatg
    11821 aactgattct ctcactggct tcgtcagaat ttgagcaggt gttttctcca cagattcaga
    11881 aaagattagt taaaattctt ttcatggaag aaaaagcagg tcagctaaaa gttcactcga
    11941 ctatatcaaa aaaaggcaga ggaagattgc tgtcctggtt ggctaagaac aatattcagg
    12001 aattatcgga cattcaagat tttaaggtgg atggctttga atattgtact tccgaatcaa
    12061 cggcaaacca acttaccttc atacgatcaa taaaaatgtg aaattatgaa aaagataacg
    12121 ttttccagcg ctaaaaaggg tagaaaaata ttaatttcta tgatataatg gatgcgttat
    12181 aggtaaaagt ctaggaaggt tgtttatgaa aaagagaagc ggacgaagta agtcgtccaa
    12241 gttcaaattg gtaaattttg cgcttttggg actttattcc attactctat gtttgttctt
    12301 agtgaccatg tatcgctata acatcctaga tttccggtat ttaaactata ttgtgacgct
    12361 tttgctagta ggagtggcag tattggctgg attattgatg tggcgtaaga aagcgcgcat
    12421 atttccagcg ctcttacttg ttttttcact ggtcatcacg tctgttggga tctatggaat
    12481 gcaagaagtt gtaaaatttt caacacgact aaattcaaat tcgacatttt cagaatatga
    12541 aatgagtatc cttgtcccag caaatagtga tattacggac gttcgtcagc ttactagtat
    12601 ccttgctcca gccgaatacg accaagataa catcaccgct ttattggatg acatatccaa
    12661 aatggaatct actcaactag caactagccc cgcgacctct tacctgacag catatcaatc
    12721 tatgttgaat ggcgagagtc aagcgatggt gttcaacgga gtttttacca atattttaga
    12781 aaatgaagat ccaggctttt cttcaaaagt gaaaaaaata tatagtttca aagtgactca
    12841 gactgttgaa acagctacta agcaggtgag tggagatagc tttaatatct atattagtgg
    12901 tattgatgct tatggaccga tttctacggt ctctcgttca gatgtcaata tcattatgac
    12961 tgtcaatcgt gcgacacata agattttatt gacaactact ccacgagatt catacgttgc
    13021 tatcgcagat ggcgggcaaa atcaatacga taaactaaca catgctggta tctacggtgt
    13081 caatgcttct gtgcacacct tagaaaatct ctatgggatt gacattagca attatgtgcg
    13141 gttgaacttc atttccttcc ttcaattaat cgacttggtg ggtggaattg atgtatataa
    13201 cgatcaagaa tttacaagtt tacatgggaa ttatcatttc cctgttggac aagttcattt
    13261 aaactcagac caagcattag gcttcgttcg agagcgctac tctttaacag gaggtgacaa
    13321 tgaccgtggt aaaaaccagg aaaaagtgat tgctgccttg attaaaaaga tgagtacgcc
    13381 agagaatcta aaaaattacc aggcaatcct atctggattg gaaggctcaa ttcaaacgga
    13441 tttgagctta gaaacgatta tgagtttagt gaatacccaa ctagaatcag gaacacaatt
    13501 tacagtagag tcacaagcat tgacaggaac aggacgctca gacttatctt cttatgcgat
    13561 gcctggatca caactttata tgatggaaat taaccaagat agtctggagc aatcaaaggc
    13621 agcgattcag tccgtacttg ttgaaaaata aagattttag gagaaaatat gaacaatcaa
    13681 gaagtaaatg caatcgaaat cgatgtttta ttcttactaa aaacaatttg gagaaagaaa
    13741 tttttaattc tcttaactgc agtgttgact gcggggttgg catttgtcta cagtagtttt
    13801 ttagtgacac ctcaatatga ctccactacc cgtatctatg tagtgagtca aaatgttgaa
    13861 gccggtgcgg gcttgactaa ccaagagtta caagcgggta cctatttggc aaaagactat
    13921 cgggaaatta tcctatcaca agatgtattg acacaagtag caacggaatt gaatctgaaa
    13981 gagagtttga aagaaaaaat atcagtttct attcctgttg atactcgtat cgtttctatt
    14041 tctgtgcgtg atgcggatcc aaatgaagcg gcacgtattg caaatagcct tcgcaccttt
    14101 gcagtgcaaa aggttgttga ggtcaccaag gtaagcgatg tgacgacact tgaagaagca
    14161 gtcccagcgg aagaaccaac cactccaaat acaaaacgaa atatcttgct tggtttatta
    14221 gctggaggta tcttggcaac aggtcttgta ctggttatgg aggttttgga tgaccgtgta
    14281 aaacgtcctc aggacatcga agaggtaatg ggattgacat tgctaggtat agtaccagat
    14341 tcgaagaaat taaaatagga gaacaatatg gcgatgttag aaattgcacg tacaaaaaga
    14401 gagggaataa ataaaaccga ggagtatttc aatgctatcc gtaccaatat tcagcttagc
    14461 ggagcagata ttaaggttgt tggtattacc tctgttaaat cgaatgaagg taagagtaca
    14521 actgcggcta gtctcgctat tgcctatgct cgttcaggtt ataagaccgt cttggtggat
    14581 gcagatatcc gaaattcagt catgcctggt ttcttcaagc caattacaaa gattacaggt
    14641 ttgacggatt acctagcagg gacaacagac ttgtctcaag gattatgcga tacagatatt
    14701 ccaaacttga ccgtaattga gtcaggaaag gtttctccca accctactgc ccttttacaa
    14761 agtaagaatt ttgaaaatct acttgcgact cttcgtcgct attatgatta tgttatcgtt
    14821 gactgtccac cattaggact ggtaattgat gcagctatca ttgcacaaaa atgtgatgcg
    14881 atggttgcag tagtagaagc aggcaatgtt aagtgctcat ctttgaaaaa agtaaaagag
    14941 cagttggaac aaacaggcac accgttctta ggcgttatct tgaacaaata tgatattacc
    15001 actgagaagt atagtgaata cggaaattac ggcaaaaaag cctaatttct cagataacat
    15061 aagtttgata agtaggtatt aatatgattg atatccattc gcatatcata tttggtgtgg
    15121 atgacggtcc caaaactatt gaagagagcc tgagtttgat aagcgaagct tatcgtcaag
    15181 gtgttcgcta tatcgtagcg acatctcata gacgaaaagg gatgtttgaa acaccagaaa
    15241 aaatcatcat gattaacttt cttcaactta aagaggcagt agcagaagtt tatcctgaaa
    15301 tacgattgtg ctatggtgct gaattgtatt atagtaaaga tatcttaagc aaacttgaaa
    15361 aaaagaaagt acccacactt aatggctcgc gctatattct cttggagttc agtacgaata
    15421 ctccttggaa agagattcaa gaagcagtga acgaaatgac gctacttggg ctaactcccg
    15481 tacttgccca tatagagcgt tatgatgctc tggcatttca gtcagagaga gtagaagagc
    15541 taattgacaa gggatgctac actcaggtaa atagtaacca tgtgttgaag cctgctttaa
    15601 ttggcgaacg agcaaaagaa tttaaaaaac gtactcgata ttttttagag caggatttag
    15661 tacattgtgt tgctagcgat atgcataatt tatatagtag acctccgttt atgagggagg
    15721 cgtatcagct tgtaaaaaaa gagtatggtg aggatagagc gaaggctttg ttcaagaaaa
    15781 atcctttgtt gatattgaaa aatcaagtac agtaacctca tagaaatagt ggaggagcta
    15841 tgaatattga aataggatat cgccaaacga aattggcatt gtttgatatg atagcagttg
    15901 cgatttctgc aatcttaaca agtcatatac caaatgctga tttaaatcgt tctggaattt
    15961 ttatcataat gatggttcat tattttgcat tttttatatc tcgtatgccg gttgaatttg
    16021 agtatagagg taatctgata gagtttga
//
LOCUS       DAZQCJ010000040        15685 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000040 DAZQCJ010000000
VERSION     DAZQCJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15685
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.041"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..452
                     /locus_tag="ACHU66_001156"
     CDS             <1..452
                     /locus_tag="ACHU66_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389445.1"
                     /translation="ILGEFLSIFIYSVVFMIALLERYQWFSKTSYNSLYGNVDFSNPL
                     ERYVELFVAHSRKFGWLVLTPVFLYRIFFSGGENLYRNDLIRTVSILSFPIIGLVGLY
                     FVIALGASQIKGYYISKYMEDYRILSGYSIEDWYGKKSRRYKESLGK"
     gene            541..1401
                     /locus_tag="ACHU66_001157"
     CDS             541..1401
                     /locus_tag="ACHU66_001157"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389446.1"
                     /translation="MKKTIWNASLEESMNLVTDKYIKTSMDDVNQYGHKKKILGFFTM
                     VLFIGLLSLIGSYIDKESGVLLFLKAELLFRIPLVLSLLFLFCYLMELRKVKEYRVLS
                     YYIFNRYMFLVMSCFILQFWLICAVGFAILWGSVLSIIVNVGIFSIVVSERLISFVKK
                     TEGVLYQGQPSNNILYKFLEKFVAFSKRYGGGLVVLLLVSRFVFKNSFQSASGEGIYH
                     NDFLRSLAMTFSVFFPLLPFYFSVAIATSCIEGYYLEKYSEDYRQLSGYSVEDWYGKK
                     SRRYKESLGK"
     gene            complement(1614..2483)
                     /locus_tag="ACHU66_001158"
     CDS             complement(1614..2483)
                     /locus_tag="ACHU66_001158"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFR3389447.1"
                     /translation="MKIDIRIPQAFPQLTVKEVLEDYFLIPRKIRHFLRTKKHVRVNG
                     ELINWQSPIAAGDLLELTFDQEDYPEKSIPLGQANLVEELYQDEHLIIVNKPEGMKTH
                     GNDPTEIALLNHVSAYVGQTCYVVHRLDMETSGAILFAKNPFILPILNRLLEDKVIYR
                     DYLALCQGQLRKTDWTITDKIGRDRHDRRKRVVDNRKGQVALTQVRLLKTLSKNSLVT
                     CRLQTGRTHQIRVHLAHHDHALIGDPLYSQVAAPRLMLHAQKLSLTHPLTLEEIRVEA
                     RSESFEKVLKNMK"
     gene            complement(2485..3453)
                     /locus_tag="ACHU66_001159"
     CDS             complement(2485..3453)
                     /locus_tag="ACHU66_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR3389448.1"
                     /translation="MTQVYDITIIGGGPVGLFAAFYAHLRQAKVKIIDSLPQLGGQPA
                     ILYPEKAILDIPAFPSLTGQELTDNLLAQLAPFDTTICLNETLTAIEPGEVISLTTNK
                     GSHQTKTLIIAMGGGAFKPRPLEIDGADSFDNVHYHVSNIQQYADKDIVVLGGGDSAV
                     DWSLAFEKIAKTTQIVHRRDNFRALEHSVEELKQSSVTIHTPFIPKGLSGENGRASAI
                     DFDKVKSEDKLTLSFDHLFVNYGFKSSVGTLKEWGLELNRHRIVVNSKQETSVPGIYA
                     IGDCCFYEGKVDLIATGLGEAPTAVNNAMNYLNPNEKVQPKHSTSL"
     gene            3606..5819
                     /gene="pbp2a"
                     /locus_tag="ACHU66_001160"
     CDS             3606..5819
                     /gene="pbp2a"
                     /locus_tag="ACHU66_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2A"
                     /protein_id="HFR3389449.1"
                     /translation="MDDLQHQPIEVPEDFQIDHEDRPSRRRRGNRGSVDKPKKKSRIP
                     EPIRKFWRRYQLTKILIILMVLTVLTVGGYLFFLAKTANVGDLQQALKATTIIYDKDG
                     AEAGTLSGQKGTYVELDAVSDNLENAVIATEDRSFYKNSGINYQRTILAALTLGRSGG
                     GSTITQQLAKNAFLTQDQTISRKAREFFLALEINKKYSKQEILTMYLNNAYFGNGVWG
                     VEDASQKYFGIPASDLTLEQAAVIAGMLKGPEIYNPYYSLENAVNRRDTVLQNMVNAG
                     YIDQASADAGFQVDLASQLSDTYTGKQSNYSYPSYFDAVIAEAIERYGLKESDIINNG
                     YRIYTEMDQNSQASMQVIFNDETMFPTSSFDGTHAQAASVALDPRTGGVRALVGRVNS
                     SEDAVFRTFNFATQAKRSPGSTIKPLVAYAPAVAAGWSIDMALDNHTETYGDYTLHNY
                     DYSTSDTIPMYQALALSYNLPVAYIVNTLGIDKAFEYGQKFGLDMENVEKVLGVSIGS
                     GVETNPLQMAQAYATFANKGVMKDAHLITRIETASGKVLAEHRETSTKVIDRSVADKM
                     TAMMLGTYTNGTGVTADTANYHIAGKTGTTETSFDVNLVNDQWHIAYTPDLVISQWVG
                     FEQTDENHYIDSSSYWKAPSVFQTVTNSILPYTAGTKFEVENAYAQNGIVEVAPDEAA
                     QTESSLGQEQVQEISEQAKNLIEQTKQNLIDAKLPERAKTLWDNITSWFGELSNP"
     gene            5853..6005
                     /gene="rpmG"
                     /locus_tag="ACHU66_001161"
     CDS             5853..6005
                     /gene="rpmG"
                     /locus_tag="ACHU66_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR3389450.1"
                     /translation="MALKKASLACTVCGSRNYSISLSSNPKPTRLEVNKFCKHCGQYT
                     VHKETR"
     gene            6015..6191
                     /gene="secE"
                     /locus_tag="ACHU66_001162"
     CDS             6015..6191
                     /gene="secE"
                     /locus_tag="ACHU66_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR3389451.1"
                     /translation="MKFIKDIIRILKDTSWPTRKQSWTDFVSVVEYTAFFVAVVYLFD
                     LILSRGIMSLINLF"
     gene            6301..6840
                     /gene="nusG"
                     /locus_tag="ACHU66_001163"
     CDS             6301..6840
                     /gene="nusG"
                     /locus_tag="ACHU66_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR3389452.1"
                     /translation="MYDSFDKGWFVLQTYSGYENKVKENLLQRAHTYNMLENILRVEI
                     PTQTVQVEKNGEVKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRQILVSMGQTVQEFDIDVKVGDTVRIIDGAFTDYTGKITEIDNNKVKMVI
                     SMFGNDTIAEVNLSQIAEL"
     gene            6984..12053
                     /locus_tag="ACHU66_001164"
     CDS             6984..12053
                     /locus_tag="ACHU66_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HFR3389453.1"
                     /translation="MKQKWSQIENKQRFSIKKLSVGVASVSIGFFITGVPMVQADTSG
                     EGLESTVAVATDMDSRQNSAVEKKEDGPLSDEPVKTEQVDEPVAEEGVVEEVVDTEAG
                     EESGLLTDQAATEIKTTAGKTTDESKEKEDISGKEASAPQTIPQESQLEPEEVTRGRY
                     ILQFSEENRNLVLDKLKKIDGVKIVHEYKEVLTGASVEVGKESLSDVKAITELTSLEE
                     SRRIRPTLHTAKQLVGALKASSKYQTDGRGMVIAVIDSGLDIKHKDMRLDDGVIPKIK
                     DITPSTTGTYTLKVPHGYNYVSGNDNLYDDTHEPHGMHIAGTLAGNATDEEVASKKGV
                     DGIAPNAQLLVYKIFSNDPKNYKAETEDAAYAAIEDAIKHGADVISLSVGYYDSGLPG
                     NAYYTIAKRAAEKGIIITAAIGNAGASSSDTSFDLHTNNALGAVDTATTVGVAATPAV
                     IAVGSARNTHLVQREFMLNGQSFGYYPIGYTTLTEGKYEFVDAGNGHWEEVQGLDLAG
                     KVAVIKKDKFDLKDAVRNLKFKDVAGIIVINTDQGWNKDYYRTHQLLVDDKTLLSYSS
                     IWGISLSGEDGRRLLEVANQSQGNTGLVLKPTIGMKKLIEVPTVSGFSSWGPTVNLEL
                     KPEIVAPGEDVYATLNDNRYGSMSGTSMASPIVAGASALLLPRIRQMTPPEGMTRMDL
                     LRIILMNTATPLVDVLDSSGHALENSPRQQGAGLLQIDRAFETDVILHHRLKGGVELK
                     EIGRETEFEVTLENLGNQQRSFAISAGKVLTSQDVPVDRIGRSGKVVKEIHATEIKGS
                     SIHLSEQSIQLGPKEKRTIRLKLDAGEAKDQFAEGYIYFKSLTDGQSDISIPYFGFVG
                     DWSKERIVDAPAWETSSKLKLTSVLSSYKHNKSGRYIELGREKIQDNQSPLNPDNIAI
                     QNQHSDSQIGNAFVRFALLRDITNYDLDIVKEATEDAPVLRRIDTGTMLSRVRYVDYF
                     ESLSEYFKLRTPIELHRWDGKVYDASKDENIPAPEGQYFFRLRVKNKENGAYQYTYLP
                     VKIDNQKPEIVAIDTNRLTSHRELVVTAKDNNKVWEVRANLNGEDLLVEKVVDDAGQL
                     HYHLKEVELPLDAKNHLRVEVMDIAGNVVAVEKDLVAPVIQFKNLEDLMAIRSKKTVE
                     IKANVSAQVSNVQANLDAQAVNYSLENGQLSLQIPEQSDGRHSFELILKDKDGKLIYT
                     KTLNYLVDNEKPTIDLDIEKDEEVIQIGKNGRFTLKGKVSDNVSLPKNIKLYYSNLDI
                     GKGELKIIDVKEDGSFEQDFFKSDFPRAIMLTAVDEKGNKLKDLRINTSPESLDEEEE
                     TEVPITVNNWLIDPIRFNKESLGRELDSGLVDFKKQEDGTYLFTFEIEAETDQAHSVR
                     INGGEKRYFEDGKLTYPVTLIEEGNVVDISVYNEADELTYTKKYQMLVDTENPVLQLE
                     NEVLPLERQVVDSEEDEDEENQYAGVLLADADGHLTLTGSAKDNGIYWSLKINEDFVA
                     RGGFWRQYGNNEKAFRYELHSLKDGDTVKLDLSDSFGNALVKKYKVRLNDKEVSEQVP
                     EKDLHVEQSDKDQAPSIPVPKSEAHVPMPKEENSLAPQTESTEIALLTGDTREDGVEH
                     LVRFTKHEEALGISDERIEVSVPHREFFERSGRGETGALAADTSGKLPQTGDSLGSVF
                     ISALLGLFGGAMVLGNLKRKE"
     gene            complement(12096..12166)
                     /locus_tag="ACHU66_001165"
     tRNA            complement(12096..12166)
                     /locus_tag="ACHU66_001165"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12132..12134),aa:Cys,seq:gca)
     gene            12707..13219
                     /locus_tag="ACHU66_001166"
     CDS             12707..13219
                     /locus_tag="ACHU66_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR3389454.1"
                     /translation="MIVLITGASHTGKTVLAQKLLEKYHYPYLSIDHLKMGLIRSGNT
                     KLSVEEDDKLTAYLWPIVREMIKTAIENEQNLIVEGIYIPFDWATDFAIAYLDHIRYY
                     CLVMSENYIQKHFGSIKKHARLIENRIDDEWCTMESVLEENTRFFRRAKKYDANILWI
                     DESYEIEIDL"
     gene            13566..14342
                     /gene="rpsB"
                     /locus_tag="ACHU66_001167"
     CDS             13566..14342
                     /gene="rpsB"
                     /locus_tag="ACHU66_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR3389455.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFIRDAAANDAVILFVGTKKQAAEAVKDEAIRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEINRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIADMPRIPDV
                     MFVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKMA
                     DAIIEGNQGEDSVAAVEAELAAEPASTESIEELVEVVEGK"
     gene            14595..15635
                     /gene="tsf"
                     /locus_tag="ACHU66_001168"
     CDS             14595..15635
                     /gene="tsf"
                     /locus_tag="ACHU66_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR3389456.1"
                     /translation="MAEITAALVKELREKSGAGVMDAKKALVETEGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVDGNVAAVVEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPADNEAALKLAMPSGETLEEAYVNATATIGEKISFRRFALVEKTDAQAFGAYQHNG
                     GRIGVISVVEGGDETLAKQISMHIAAMKPTVLSYTELDEQFVKDELAQINHKIEQDNE
                     SRAMVDKPALPLLKYGSKAQLTDEVIAAAEEAIKAELAAEGKPEKIWDKIIPGKMDRF
                     LLDNTQVDQAYTLLAQVYIMDDSKTVEAYLNSVNASVVEFARFEVGEGIEKASNDFEA
                     EVAATMAAALGK"
BASE COUNT         4864 a         2743 c         3711 g         4367 t
ORIGIN      
        1 tgatacttgg tgagttcttg tcaatattca tctattcggt ggttttcatg attgcccttc
       61 ttgaaagata tcaatggttt agtaagactt cgtacaatag tttgtatggt aatgtagatt
      121 tcagtaaccc actagaaaga tatgtggagc tttttgttgc tcattctaga aaatttggtt
      181 ggctcgttct tactccagtt tttctgtata ggatattttt ttccggcgga gaaaatttgt
      241 atagaaatga tttaataaga acggttagca tcctttcgtt tcctattatt ggattggttg
      301 gtttatattt tgttattgca cttggtgcaa gtcagataaa aggctattat atcagtaaat
      361 atatggaaga ttatcgtatt ctctcaggat atagcattga agactggtat ggtaaaaagt
      421 ctaggcgata taaggaaagt ttgggaaagt gataaataca agcaggaaat attaaaatta
      481 gtagcaaaaa gtgtatttaa ctaagagcta agactatttg ctattggtta aggagaaaac
      541 atgaaaaaaa caatatggaa tgcttcttta gaagagagta tgaatcttgt aacagataaa
      601 tacatcaaaa cttctatgga tgatgtgaat caatatggac ataagaagaa aattttaggt
      661 ttttttacga tggttctttt catcgggcta ctcagtttaa ttggaagcta tattgataag
      721 gaatctggag tattgttgtt tttaaaagct gaactattat ttcgaatccc attagtacta
      781 agtttgttgt ttttattctg ttatctgatg gaattgagaa aagtcaagga gtatagagtt
      841 ctatcctact atatttttaa taggtatatg tttttggtga tgagttgttt tattttacaa
      901 ttttggctga tatgtgctgt aggatttgcg attctatggg gttcagttct gtcaattatt
      961 gtcaatgtcg gtatttttag tatagtggta tcagaacgtc ttatttcgtt tgtaaagaaa
     1021 acagaaggag tattgtatca aggacaacca tcaaataaca tcttgtacaa attcctggaa
     1081 aaatttgttg ctttttcaaa aagatatggt ggtggtttag ttgttcttct ccttgttagt
     1141 cgttttgtat ttaaaaatag ctttcaaagt gcaagtggag aaggcattta ccataatgat
     1201 tttttacgct ccctggcgat gacatttagt gtttttttcc ccctcctccc gttttatttc
     1261 tctgtagcta ttgctactag ttgtatagag ggatattact tggagaaata ttccgaagat
     1321 taccgtcaac tatctggtta ttccgtagaa gactggtatg gtaaaaagtc taggcgatat
     1381 aaggaaagtt tgggaaagta ggttttgttt agatcatatt agtttacttt tggtttatgg
     1441 ctaagcaatg tattccaaaa tcccaagttg cataattttt tgatgcttga gctattaact
     1501 tgtaaaccca gacaaaatta ccttgtctgg attttttggt tagtggaagc ataaaaaaat
     1561 tcactggatt agtccagtga aatagggata tagcactcta agggtgcagc tatttatttc
     1621 atgtttttca agactttctc aaaactctcc gaccgtgctt ccacgcggat ttcttccaag
     1681 gtcagcgggt gggtcaggct gagtttttgg gcgtggagca taaggcgagg tgctgcgact
     1741 tgactgtaga gcggatcacc aatcagggcg tgatcgtggt gggctagatg gacccggatt
     1801 tgatgggttc ggcctgtctg aaggcggcag gttactagac tatttttgct aagagtcttc
     1861 aagaggcgga cctgggtcag tgccacctgt cccttgcggt tatcgaccac ccgctttctg
     1921 cggtcgtggc ggtcacggcc gattttatcg gtaatggtcc agtctgtctt tcttagttgg
     1981 ccttggcaga gagctagata gtcacgatag atgaccttgt cttccaagag ccgattgaga
     2041 atgggcagga taaagggatt ttttgcaaag agaatggctc cgctagtttc catatcaagc
     2101 ctgtggacta catagcaggt ctggccgaca taggcagaga catggttgag cagggcaatt
     2161 tccgtcggat cattgccgtg ggttttcatg ccttctggct tgttgacgat aatcaaatgc
     2221 tcatcctgat agagttcctc caccaagttc gcctgtccta gaggaatgct tttttcagga
     2281 taatcttcct gatcaaaggt caattctagc aggtctcccg cagcaatcgg gctctgccag
     2341 ttaatcagct caccgttgac acggacgtgc ttcttggtgc ggaggaagtg gcggattttt
     2401 ctgggaatga gaaaataatc ttccaagacc tctttgacag tcagttgtgg aaaggcttgg
     2461 ggaatacgaa tgtcaatttt catcttatag gctggtcgag tgctttggtt gcactttttc
     2521 gtttggattg aggtagttca tggcattgtt aacagcggtt ggggcttcgc ccagtccggt
     2581 cgcaatcagg tcaactttgc cttcatagaa acaacagtca ccgatagcat agatgccagg
     2641 gacagaggtt tcttgcttgc tgttgaccac gatgcggtgg cgattgagct ccaatcccca
     2701 ttctttcagc gtgccgacag atgatttgaa accgtagttg acaaagaggt ggtcaaaaga
     2761 cagggtcagc ttgtcctcgc tttttacctt atcgaaatca atggcagaag ctctgccatt
     2821 ttccccagaa agccctttag ggataaaagg tgtgtgaata gtaacgctgg actgcttgag
     2881 ctcctccacg ctgtgctcga gggctcggaa gttatcgcgt cggtggacaa tctgggtggt
     2941 cttggcgatt ttttcaaagg ccagggacca atcgaccgca gagtcaccac cacccaatac
     3001 gacgatgtcc ttgtcggcat actgctggat attagacacg tggtagtgga cgttgtcaaa
     3061 gctgtcagca ccatcaatct ccagcggacg tggcttgaag gccccgccac ccatggcgat
     3121 aatcagggtc ttggtttggt gactgccttt attggtcgtc aggctaatga cctccccagg
     3181 ctcaatggca gtcagggttt cattgaggca gatggtggtg tcaaatggag ccagctgggc
     3241 aaggaggttg tcggtcaatt cctgtccggt taggcttgga aaggctggaa tgtccaaaat
     3301 ggccttctca ggatagagaa tggcaggctg gccacccagt tggggaaggg agtcaatgat
     3361 tttgaccttg gcttgacgga gatgagcgta gaaggcggcg aagagaccga ctggtccgcc
     3421 accgatgata gtaatgtcat atacttgtgt catgggaatc ctcttctttg ttcagaatga
     3481 ccctatttta tcatattttt ggaaaaaatt ccctgtctgt tgtcacaagt tatcgcaggc
     3541 ttttttctgc tagtttttag gaaatggtgt ataatggtac aaaattcact agaaagaggt
     3601 aaagtatgga cgatttgcaa catcagccga ttgaagttcc agaagatttc caaattgatc
     3661 atgaagaccg acctagccgt cgccgcaggg gaaatcgtgg ctctgttgat aaaccaaaga
     3721 aaaaatcacg tattccggag ccgattcgta aattttggcg ccgttatcaa ctgactaaaa
     3781 ttttaatcat tttaatggtc ctgacagttc ttacagtagg tggctatcta tttttccttg
     3841 caaagacagc taatgttggt gatttacagc aggcgctaaa ggctacaacg attatttacg
     3901 ataaagatgg tgcggaagct gggacattgt ctggtcagaa ggggacctat gtagagctgg
     3961 atgctgtctc tgataattta gagaatgccg ttatcgctac agaagatcgg agcttctaca
     4021 aaaattcagg cattaattac cagcgtacaa ttttagccgc cttgacgtta ggtcggtctg
     4081 ggggtggttc aactattacc cagcaattgg caaaaaatgc ctttctaact caggatcaga
     4141 ccatcagtcg aaaggctcga gaattctttt tggctctgga aatcaataaa aagtacagca
     4201 agcaagaaat tttgaccatg tatctcaaca atgcctactt tggaaacggg gtctggggag
     4261 tggaagatgc aagccaaaag tacttcggta ttccagccag tgatcttact cttgagcaag
     4321 cggccgtcat tgcaggtatg cttaagggac ctgagattta taatccttat tattctctag
     4381 aaaatgcagt taatcgtcgg gatacagttc tgcaaaatat ggtcaatgct ggttatattg
     4441 atcaggcgtc tgcagatgca ggtttccaag tagacctagc cagtcagctt tcagatacct
     4501 atacagggaa acaaagcaat tattcctatc catcctattt tgatgctgtg attgctgagg
     4561 cgattgagcg atacggactg aaagaatcag acattattaa taacggctat aggatttaca
     4621 cagagatgga tcaaaattct caagccagca tgcaggtgat ttttaatgat gagactatgt
     4681 tcccaacatc aagttttgat ggtacccatg ctcaggctgc cagcgtagct cttgatccta
     4741 ggactggtgg agttcgcgcc ttggttggtc gagttaacag ttcagaggat gcggtctttc
     4801 ggaccttcaa ctttgcgaca caagccaagc gcagtcctgg ttcaacaatc aagcctcttg
     4861 ttgcctatgc tccagcagta gcggcgggct ggtcgattga tatggctctg gataaccata
     4921 cagaaaccta cggcgattac accttacata actatgatta ttcgacttct gataccattc
     4981 caatgtatca agctttggct ctctcttaca atttaccagt agcttatata gtgaacacct
     5041 tgggtattga caaagccttc gagtatggac aaaagtttgg acttgatatg gaaaatgtcg
     5101 agaaagtttt gggtgtttca attggttccg gagtggagac caatcctctg caaatggccc
     5161 aggcctacgc tacttttgcg aataagggtg tgatgaagga tgcccacctc atcactcgta
     5221 ttgaaacggc tagtggaaag gttttagcag aacatcgaga aacaagtacc aaggtgattg
     5281 accgctctgt cgcagataag atgacagcaa tgatgcttgg tacctatacc aatggtacgg
     5341 gagtgacagc agatacggcc aattatcata tcgctgggaa aacaggaaca accgaaacga
     5401 gttttgatgt caatctcgtc aatgaccagt ggcatattgc ctacacacct gatttggtca
     5461 tcagtcagtg ggttggtttt gagcagacag atgaaaatca ttatatagat agcagtagct
     5521 actggaaggc tccgtcggtc ttccagactg taaccaattc aatcttgcca tatactgcag
     5581 gaactaagtt cgaagtagaa aacgcctatg cacaaaatgg tattgtagaa gtagctccag
     5641 atgaagcagc gcaaactgaa tcaagccttg gtcaagaaca ggtacaagaa atttcagaac
     5701 aggcgaaaaa cttgattgag caaaccaaac aaaatctaat cgatgccaag ttgccagaac
     5761 gagcaaaaac cttgtgggat aacattacgt cttggtttgg agagttgtca aatccataaa
     5821 aacatggtag aatgaaaagg atggagggct ttatggcact aaaaaaagca agcctagcct
     5881 gtactgtttg tggttctcga aactattcaa tcagcctttc gagcaatccc aagccaacac
     5941 ggttagaagt aaataaattt tgtaaacact gtggacaata cactgttcac aaggaaacca
     6001 gataggagtc acgcatgaaa tttatcaaag acattatccg cattttaaaa gatacgtctt
     6061 ggccaactcg taagcagagc tggacggatt tcgtgtctgt agtggaatat actgcctttt
     6121 tcgtagcggt ggtttacctc tttgacttga ttttgtcacg aggaatcatg agcttgatca
     6181 atcttttcta gaaaatactt gccatttgta aaatggaagt gatataataa ggaaaagcga
     6241 aagccttcgg gcttttttta gcgtctaaaa attccgtgtt tccaaagaaa ggaagtaaca
     6301 atgtacgata gttttgataa aggctggttt gttttacaga cctactcagg atatgaaaat
     6361 aaagtgaagg aaaacttgct ccaacgtgct catacttata acatgttgga aaacatcctt
     6421 cgtgtagaaa ttccaactca gactgttcag gttgaaaaga acggtgaggt caaggaagtg
     6481 gaagaaaacc gtttccctgg ttatgttttg gttgagatgg ttatgaccga tgaagcttgg
     6541 tttgtggttc gtaatacgcc aaatgttaca ggtttcgtgg gttcacacgg taaccgttca
     6601 aaaccaactc cacttttgga agaagaaatt cgtcaaatct tggtgtctat gggacaaacc
     6661 gttcaagaat ttgatattga tgtcaaagtt ggcgacactg ttcgtatcat cgacggtgcc
     6721 ttcacagact acacaggtaa aattacagaa atcgacaaca acaaggttaa aatggttatc
     6781 tctatgtttg gtaatgatac cattgctgaa gttaatttga gccagattgc tgagttgtaa
     6841 taagttgtta aagcgcaggg aagcctgtgc tttttcttgt ttttgagggc taattcaaaa
     6901 aaaacttgcc aaagatttaa aaattttata gaattaagta tacctaactt ataaattaat
     6961 taatcttaaa aaacggaggt tttatgaaac agaagtggtc tcagattgag aataaacaac
     7021 gctttagtat caagaagtta agtgtcggtg tggcatcggt atcaatagga ttttttatca
     7081 cgggtgttcc gatggttcaa gcagatacta gtggagaagg attggagtct acagttgcgg
     7141 tcgcaacgga tatggatagt aggcaaaatt ctgcggtaga gaaaaaagaa gatggaccat
     7201 tatcagatga gccagttaaa acggagcagg tggatgaacc agttgctgaa gagggagtgg
     7261 tcgaagaagt tgtagataca gaggcgggtg aagaatcagg tcttctcaca gatcaagctg
     7321 cgaccgagat aaaaactaca gctggtaaga caacagatga gtcaaaggaa aaagaagaca
     7381 ttagcggaaa agaagctagt gctcctcaaa caatcccgca ggaatcacag cttgagccag
     7441 aagaggtgac aagagggcgc tatattttac aattttccga agagaaccga aatcttgtat
     7501 tggataaatt aaagaaaatt gatggcgtta aaattgttca tgagtataag gaggttttaa
     7561 cgggagcatc agtagaggta gggaaagaaa gtttgtctga tgtgaaggca attaccgaat
     7621 taacctcttt agaagagagt cgccgtatcc gaccgactct tcatactgct aaacagctgg
     7681 ttggtgcctt aaaagcaagt tctaaatatc agacagatgg tcgcggaatg gtgattgcag
     7741 tcattgactc cggtttggat attaagcaca aagacatgag gttggatgat ggtgtcattc
     7801 ctaaaatcaa ggacattact ccatctacga caggaacata cacactgaaa gttccccatg
     7861 gatataatta tgtatctggt aacgataatc tctacgatga tacccacgaa ccacatggta
     7921 tgcacatagc aggaactctg gctggaaatg caacagacga agaagttgcg tctaaaaagg
     7981 gagttgatgg gattgctccc aacgcacaac tgctagtcta caagattttt tcaaatgacc
     8041 ctaaaaatta taaagctgag acggaggatg ctgcttatgc tgctattgaa gatgctatca
     8101 aacacggggc agatgtcatc agcttgagtg ttggttatta tgatagcggg ttaccaggga
     8161 atgcttacta cacgattgct aagagggcag cagaaaaggg aattatcatt acagcggcta
     8221 ttggtaatgc tggtgcctcc tcttcagaca cctcctttga cctgcataca aataatgcct
     8281 taggagctgt ggatacggca acaacagttg gtgtggcagc gactcccgca gttatcgcgg
     8341 ttggttcggc gagaaatacc catcttgttc aacgagaatt tatgctgaat ggacagtctt
     8401 ttggttacta tcctattggc tatacaacgc ttacagaggg aaaatatgaa tttgtagatg
     8461 ctggaaatgg tcattgggaa gaagtgcaag ggcttgattt ggctggtaaa gtagcggtta
     8521 tcaaaaaaga taagtttgac ttgaaagatg ctgttcgtaa tttgaaattc aaggatgttg
     8581 ctggaattat tgtgataaat actgatcagg gatggaacaa ggattattat aggacccatc
     8641 agttgttggt agatgataag actttgcttt cctactcctc tatctgggga attagtctta
     8701 gcggggaaga tggaaggcga ttattagaag ttgccaacca atcgcaaggt aacactggtt
     8761 tggttcttaa acctacaatt ggaatgaaga agttgattga agtgccgact gtatcaggct
     8821 tttctagttg gggacctacg gtcaacttag aattgaagcc agagattgtg gcgcctggag
     8881 aggatgttta tgcaaccttg aatgacaatc gttatggctc catgtcaggg acttcgatgg
     8941 cttcgccaat tgtggcgggt gccagcgcac ttttattgcc acggattcgc caaatgacac
     9001 caccagaagg catgactagg atggatttgc tgagaatcat tttgatgaat actgctaccc
     9061 ctttggttga tgttctagat tcatctgggc atgctttgga aaattctccg agacaacaag
     9121 gtgctggtct gttgcagatt gatagagcct ttgaaacaga tgtgattctt caccaccgtc
     9181 taaaaggagg ggtggaatta aaagaaatag ggcgtgagac agaatttgag gtaaccttgg
     9241 aaaatctggg aaaccaacaa agaagctttg ctatttcagc tgggaaagtg ttgactagtc
     9301 aagatgttcc tgttgataga ataggacgtt ctggaaaagt agttaaagag attcatgcga
     9361 cagaaatcaa ggggtcgagt attcatcttt ctgagcaatc cattcaacta ggtccaaaag
     9421 agaagagaac cattcgcctg aaattggatg caggagaagc gaaagaccag tttgcagaag
     9481 ggtatattta cttcaaatca ttgacggatg ggcagtctga catcagcata ccttattttg
     9541 gctttgttgg tgactggtca aaagaaagga ttgttgatgc accggcttgg gaaaccagtt
     9601 ctaaattgaa actgacctca gttttatcta gctataaaca caataagtct gggcgctata
     9661 ttgaacttgg gcgtgaaaag attcaagata atcaatcgcc tctaaatcca gacaatatcg
     9721 caattcaaaa ccagcattcg gatagtcaga ttgggaatgc ctttgttcga tttgcactat
     9781 tgagggatat taccaactat gatttggata ttgtaaaaga agctacagaa gacgctcctg
     9841 ttttaaggcg aattgataca ggaactatgc tgtctcgtgt tcgctatgta gattattttg
     9901 aaagtctttc ggagtatttc aagcttcgta ctccgataga attgcaccgt tgggatggga
     9961 aagtgtacga tgcaagcaag gatgaaaaca taccagcacc ggaaggacaa tatttcttta
    10021 gattgcgggt aaaaaataaa gaaaatgggg cctatcagta tacctatctg ccagtcaaaa
    10081 ttgataatca aaagccagaa attgtcgcga ttgataccaa tcgtctgacc agccatagag
    10141 aactagtcgt cacagccaag gataataata aagtttggga ggttcgagct aatctaaatg
    10201 gagaagatct tcttgttgaa aaggttgtag atgatgcagg tcaactacat taccatctca
    10261 aagaagtgga actgccactc gatgctaaaa atcatctccg tgttgaggtg atggatatag
    10321 cgggtaatgt ggttgctgtt gaaaaagact tggtggcgcc tgtaatccag tttaaaaatt
    10381 tggaagattt gatggcaatt cgcagtaaga aaactgtaga aattaaagcg aacgtctcag
    10441 cacaagtttc caatgtacag gctaacttgg atgctcaggc tgtgaattac tctctggaga
    10501 atggtcaact atctctccag attccagagc aatcagatgg acgtcatagt tttgaattaa
    10561 tcttgaaaga taaggatggc aagcttattt acactaaaac cttgaattat ttggtagata
    10621 atgaaaagcc gacgattgat cttgatattg aaaaagatga agaggttatc cagattggca
    10681 aaaacgggcg ttttacactg aaggggaaag tgagtgataa tgttagtctt cctaagaaca
    10741 tcaaacttta ctattccaat ctagacattg gtaagggaga gctgaagatc atagatgtaa
    10801 aagaagatgg tagttttgag caagacttct ttaaatctga ttttcctaga gctatcatgt
    10861 taactgcggt agatgagaag ggaaataaat taaaggacct tcgtatcaat acaagtccag
    10921 agagtctgga tgaggaggag gaaacagaag ttccgattac tgttaataac tggttgattg
    10981 acccgattcg tttcaataaa gaaagtcttg gtcgtgagct agatagcggg ttggtcgact
    11041 tcaaaaagca agaagatggg acttatctat ttacatttga aatagaagca gaaacggatc
    11101 aagctcattc tgtgcgcatc aatggtggtg aaaaacgcta ttttgaggat ggcaagttga
    11161 cctatccagt taccctgatt gaagagggca atgttgtgga tataagtgtg tacaatgagg
    11221 cggatgagtt gacttacacg aagaaatatc agatgttggt tgatacagaa aaccctgttt
    11281 tacagctgga aaatgaggtg ctaccattgg aaagacaggt agtggatagt gaagaagatg
    11341 aagacgagga aaatcagtat gcaggagttc tcctggcaga tgctgatggg cacttgacac
    11401 ttaccggaag cgccaaggat aatggaattt attggtcctt gaagatcaac gaagattttg
    11461 tagctcgagg aggtttctgg aggcagtatg gaaataatga aaaagccttt cgctatgagc
    11521 tacatagtct gaaagacggg gatacagtca aacttgattt gagtgacagt tttggaaatg
    11581 ctcttgtgaa gaaatataag gtacggttaa atgacaagga agtatctgag caggttcctg
    11641 aaaaagacct tcatgtggag caatcagata aggatcaagc ccctagcatt cctgttccga
    11701 aatcggaagc gcatgtacca atgccaaaag aagagaattc gcttgctccg caaacagaat
    11761 ccactgaaat cgcgttgtta actggcgata caagagaaga tggtgtggag catttggtga
    11821 gatttacaaa acatgaggag gcactaggaa tttctgatga gagaatagaa gtgtccgtac
    11881 ctcacagaga attctttgaa agaagcggaa gaggagagac aggtgcacta gcggcggata
    11941 ctagtgggaa actgcctcaa actggagata gccttggaag tgtctttata agtgctttgt
    12001 taggtctatt tggtggagct atggtcctcg gaaatctgaa gcgaaaagaa tagcaaaaaa
    12061 cctaggaaga ttcctaggtt ttttagaata ttaaaaggcg gtagacggat ttgaaccgac
    12121 gatcaagctt ttgcagagcc gtgccttacc acttggctat accgccacaa caaagaatat
    12181 tctaccgaaa aaaaagctga aagtcaagaa aaatctagtg actgtttgtg actgataaca
    12241 aatctataaa acagttgcga aaacatagga gaatacgacg aaaaagatag gagtactttt
    12301 tgagtattga tggctagtac cactctcttt gtttgataga aaaagagtgg agcaaaaaga
    12361 attaagaaac actttgggta agtgaattca tttttactgc aaggaatgtt aagggaacag
    12421 aattacgata atagtgcacc tctttaagaa agatttggag gtgatagagt ttatttttgc
    12481 taaataaaga ttaaaccaag aagaatattc catctaaaac ggttgttttc atttacagta
    12541 tattgcaaag ataactcagg cacttgatta gattaactga aaaaatgtgg gaacatgata
    12601 taataaattg agtaagagat tgaaaaatag ataggatgac aagcagtgtt tcttacaaat
    12661 tttcatataa aacatagatg ctttgtcgct tagataggag aaaatcatga ttgttttaat
    12721 aacgggagca tctcacactg ggaagactgt gctggctcaa aaattacttg aaaaatatca
    12781 ctatccttat ttatcaatag accatttgaa aatggggtta attcgtagcg gcaatactaa
    12841 actttcagtc gaagaagatg ataaacttac agcatatcta tggcctattg ttcgtgaaat
    12901 gattaaaact gccatagaaa atgagcaaaa ccttattgtt gaagggatct atatcccttt
    12961 tgattgggca accgactttg caatagccta ccttgaccat atcagatatt actgtcttgt
    13021 gatgagtgaa aactatattc agaaacactt tggaagcata aagaaacacg cacgccttat
    13081 agaaaatcgt atagatgatg aatggtgtac gatggaaagt gttttagaag aaaatactcg
    13141 attttttaga agggcgaaaa aatatgatgc aaacatccta tggattgatg agagttacga
    13201 gatagagatt gatttatgat agagagacat tgtatcacag tttatctaag ggatacaact
    13261 aagaggaagt aacatcagat aggcgttact tcctttttat ttctaagaaa tataaagtaa
    13321 aatggatgaa gttaaaaatc ataaaaaagt agtaacataa agagagacat atgccaatct
    13381 atgagcttgg cataatatag agatttgtgt actgaaatgc ttgattttca cctaaaactt
    13441 tggtatactt actaagttgt ctaaacaaca aatactcatc tcggaccaat tgtggtcttg
    13501 ttgccgaaag gttgggctgc aagtcgaagt tcgggagagg agaaaaaaca aaaggagaaa
    13561 tactcatggc agtaatttca atgaaacaac ttcttgaggc tggtgtacac ttcggtcacc
    13621 aaactcgtcg ctggaatcct aagatggcta aatacatctt cacagagcgt aacggtatcc
    13681 acgttatcga cttgcaacaa actgtaaaat tggctgacca agcttacgaa ttcatccgtg
    13741 acgctgcagc aaacgacgca gttatcttgt tcgtaggtac taaaaaacag gctgctgaag
    13801 ctgttaaaga cgaagctatc cgcgctggtc aatacttcat caaccaccgt tggttgggtg
    13861 gaactcttac aaactggggt acaatccaaa aacgtatcgc tcgtttgaaa gaaatcaacc
    13921 gtatggaaga agatggaact ttcgaagtgc ttcctaagaa agaagtagca ttgttgaaca
    13981 aacaacgtgc tcgtcttgaa aaattcttgg gcggtatcgc tgacatgcca cgcattccag
    14041 acgtaatgtt cgtagttgac ccacataaag agcaaatcgc tgttaaagaa gctaaaaaat
    14101 tgggtatccc agttgtagcg atggttgaca caaacactga tccagatgat attgatgtta
    14161 tcatcccagc taacgatgac gctatccgcg ctgttaaatt gatcacagct aaaatggctg
    14221 acgctatcat cgaaggcaac caaggtgaag acagcgtagc agcagttgaa gctgaattgg
    14281 cagctgagcc agcatctaca gaatcaatcg aagaattggt tgaagttgta gaaggaaaat
    14341 aagtaacgaa ggcgtttgga aatctgtatg aaaataggaa actgacgcag tatgcttgca
    14401 tacaaggaag tttatctttt tcactaagat tttagcctga gtacaattga ataagtaatg
    14461 aaggcgaatt aaaatcaata cggttttagc tcgcgtataa ttctacatca ttatttaaca
    14521 acaaaaaaac aatcctagag gggcagggct gagcccgctc ctctatttta taaatacaaa
    14581 caaggagaat agacatggca gaaattacag cagctctcgt taaagaattg cgtgaaaaat
    14641 caggtgctgg cgttatggac gcaaaaaaag cattggttga aactgaaggt gatatcgaaa
    14701 aagcgattga attgcttcgt gaaaaaggta tggccaaggc agctaagaaa gctgaccgtg
    14761 tagcagctga aggtttgaca ggtgtttacg ttgatggtaa cgtagcagca gttgttgaag
    14821 tgaacgctga aacagacttc gttgcgaaaa acgctcaatt cgttgagttg gtaaacacta
    14881 ctgctaaagt aattgcagaa ggcaaaccag ctgacaacga agcagctctt aaattggcta
    14941 tgccttcagg tgaaaccctt gaagaagcat acgtaaacgc aactgcaact atcggtgaga
    15001 aaatctcatt ccgtcgtttt gctttggttg aaaaaacaga tgctcaagca tttggtgcat
    15061 accaacacaa cggcggccgt atcggtgtta tctcagttgt tgaaggtggc gacgaaactc
    15121 ttgcaaaaca aatctcaatg cacatcgctg cgatgaaacc aactgttctt tcttacactg
    15181 aattggatga gcaattcgtt aaagatgagt tggcacaaat caaccacaaa atcgaacaag
    15241 acaacgaaag ccgtgcaatg gttgacaaac cagcattgcc attgttgaaa tacggttcaa
    15301 aagctcaatt gactgacgaa gtgattgcag cagctgaaga agctatcaaa gcagaattgg
    15361 cagcagaagg caaaccagaa aaaatctggg acaaaatcat cccaggtaaa atggatcgct
    15421 tcttgcttga caacacccaa gttgaccaag cttacacact tcttgcacaa gtttacatca
    15481 tggacgacag caaaacagtt gaagcttact tgaactcagt aaatgcttca gttgtagaat
    15541 ttgctcgttt cgaagtaggt gaaggtatcg agaaagcttc aaacgacttt gaagcagaag
    15601 ttgcagcgac aatggcagca gctcttggta aatagtagat agttaaaaaa gctaccttag
    15661 aaggtagctt tttctgtggg cttat
//
LOCUS       DAZQCJ010000041        15513 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000041 DAZQCJ010000000
VERSION     DAZQCJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15513
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.042"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(297..1256)
                     /locus_tag="ACHU66_001169"
     CDS             complement(297..1256)
                     /locus_tag="ACHU66_001169"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFR3389457.1"
                     /translation="MSKKIIGIDLGGTSVKLAILTTEGEIQEKWSIKTNILDDGSHIV
                     PDIIDSIQHRFETHGLTKDNFLGIGMGSPGVVDSEAGTVIGAYNLNWKTLQLVKDQFE
                     SALGLPFFIDNDANVAALGEQWVGAGNNNPNVVFMTLGTGVGGGVIAAGNLIRGVKGA
                     GGELGHITVDFDEPFACTCGKKGCLETVASATGIVNLSRRYADQYAGDAKLKQMIDDG
                     QDVTAKDVFDLAKEGDDLALIVYRHFSEYLGVACANIAAVLNPAYIVLGGGVSAAGEL
                     LLDGVRKVFAENSFPQIKESTQIVLATRGNDAGVLGAASLVLK"
     gene            complement(1352..1687)
                     /locus_tag="ACHU66_001170"
     CDS             complement(1352..1687)
                     /locus_tag="ACHU66_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389458.1"
                     /translation="MKVLVMSYMVIYLLVTLGAALFSYLKTKKMNTLRLILTILSMIL
                     LTSTLYFYSQSYHDLQMVGFALGFTFISTLFLYNGTKEGSNFTTVMLFSIGRFILHIQ
                     FLILLYLFR"
     gene            complement(1688..3556)
                     /locus_tag="ACHU66_001171"
     CDS             complement(1688..3556)
                     /locus_tag="ACHU66_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFR3389459.1"
                     /translation="MSDFIVEHLTKSVGDKTVFADLSFIIHQGDRIGIIGVNGTGKTT
                     LLDVLSGRIGFDGDVSPFRTKNAYKIAYLTQEPDFDESKTVLDTVLSSDLRETQLIRE
                     YELLLSHYDEASQARLEKVMAEMDSLNAWEIESQVKTVLSKLGLTDLNKTVVELSGGL
                     RRRVQLAQVLLGNADLLLLDEPTNHLDIDTIEWLTTFLKNTKKSVLFITHDRYFLDNV
                     ATRIFELDRASLTEYQGNYQDYVRLKAEQDERDAALRHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKGDLANKIDDSELEINFETSRIGKKVINFENVSFSYPDKPILAD
                     FNLLIQNKDRIGIVGDNGKGKSTLLNLIAGDLQADSGKVDIGETIRIGYFSQTIKGLD
                     ESKRVINFLQEVAEEAKTTSGMVSIAELLEQFLFPRNTHGTLIEKLSGGEKKRLYLLK
                     ILLQRPNVLLLDEPTNDLDIATLTVLEHFLQSFTGPVITVSHDRYFLDKVANKILAFE
                     DSGIETFFGNYTDYLDEKAFLASSSAISLEKPKEKTEKIKENKKRMSYFEKQEWATIE
                     EDIAGLEERIAAIEAEMLTCGSDFTKLSDLQKELDEKNDLLLEKYERYEYLSELEG"
     gene            complement(3549..4760)
                     /locus_tag="ACHU66_001172"
     CDS             complement(3549..4760)
                     /locus_tag="ACHU66_001172"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFR3389460.1"
                     /translation="MKLNNLPSEFQEALPILEKIKAAGYEAYFVGGSVRDAILGRPIH
                     DVDIATSSYPQETKQIFSRTIDVGIEHGTVLVLEGKKEYEITTFRTEEEYVDFRRPSQ
                     VSFVRSLEEDLKRRDFTVNAFALDEEAQIIDLFEGMTDLENRTLRAVGIPAERFNEDA
                     LRIMRGFRFAATLDFEIEPTTFEAMVKTAPLLEKISVERSFIEFDKLLMADFWRKGLR
                     AMIDSKAYNFLPDLAGKSDELETMLTSLTEEFRFSTSEQAWAMLFVCLGIDSIKSFLK
                     KWKTSNDFQRSVVKLVEIYQLRQAGPVTKQICFKYGKEFLYLVEELRQAQGLVTDFVA
                     IDKIDQALTIHDKHEIVVNGGHLMKAFDLKPGPVLGELLKEVEFQIVEGQLENEEQAI
                     MTFVKGILENE"
     gene            complement(4757..5524)
                     /gene="dapB"
                     /locus_tag="ACHU66_001173"
     CDS             complement(4757..5524)
                     /gene="dapB"
                     /locus_tag="ACHU66_001173"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR3389461.1"
                     /translation="MTIKVIIAGFKGKMGSTAVEMVKGDAALSLAALVDPFATETEVD
                     GVPVFKTKEEVASLEADVWVDFTTPKFAYENTRFALENGFAPVVGTTGFTPEEIEELT
                     ALSAEKGLGGLIAPNFAIGAILLMQFAAQAAKYFPNLEIIELHHDKKKDAPSGTAVKT
                     AELISQVRQSQTQGAADEEELIAGARGAAFDGFRIHSVRLPGLVAHQEVIFGAQGEGL
                     TIRHDSYDRISFMGGVNLGIKEVVKRSQLVYGLEHLL"
     gene            complement(5533..6384)
                     /locus_tag="ACHU66_001174"
     CDS             complement(5533..6384)
                     /locus_tag="ACHU66_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFR3389462.1"
                     /translation="MKLAVITDSSAILNVQSIERDDLFVLSIPVSIDGENYIEGQNLT
                     VEEFYQKMASSKELPKTSQPSLMELEEILAKLTEKGYTHALGLFLSSGISGFYQNIQY
                     LADEFEGLTVAFPDTKITSAPLGMMVENVLKWADAGLSFEEITGKLDQEIGKTTAFIM
                     VDDLNHLVKGGRLSNGAALLGNLLSIKPILYFTGEGKIEVYEKVRTEKKAIKRLLEIL
                     QEQTTEGQYQIAIIHANAPQKAENFKQQLEEAGVGSDLPIVSFGSVIGTHLGEGAVAF
                     GISPIIE"
     gene            complement(6386..6760)
                     /locus_tag="ACHU66_001175"
     CDS             complement(6386..6760)
                     /locus_tag="ACHU66_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFR3389463.1"
                     /translation="MEIIRDKEFVNQYHFDARNHAWEKENGIPETKLKVDFQLIEQNR
                     AENRTSMITILRFMIVLDHFVISGAMSQAVHLPNRLVEEPTEFTDEEKRVLVEPLLDI
                     LKRMTYEVTEIAFDAPGVNLEF"
     gene            complement(6856..8811)
                     /locus_tag="ACHU66_001176"
     CDS             complement(6856..8811)
                     /locus_tag="ACHU66_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFR3389464.1"
                     /translation="MKIQDVLRKDVMLLDLQATSKEAVIDEMIASLVDKGYVTDFDVF
                     KTGIMNREAQTTTGLGDGIAMPHAKNAAVKEATVLFAKSNNGVDYASLDGQPTDLFFM
                     IAAPEGANDTHLAALAELSKYLMKAGFADRLRAATNPEEVIAVFDTAEAADKAVEEVV
                     AAPSGDRPFIVAVTACTTGIAHTYMAEEALKKQAAEMGVGIKVETNGASGVGNKLTAE
                     DIKNAAGVIIAADKAVDMPRFNGKPLVSRPVAAGIKQPEELINIILNGKAAAYTASEG
                     AATVESSEKLSLGKAFYKHLMSGVSQMLPFVIGGGILIALAFLFDGALGVPQDSLGSL
                     GSYHEIAAMFMKIGGAAFGFMLPLLAGYIAYSIAEKPGLVAGFVAGAIASSGLAFGKI
                     PYAAGGEETLALAGVSSGFLGALVGGFLAGGVVLVLRNALRNLPKSLQGLNAILLLPL
                     LGTAITGFLMFFVNIPMAAINTGMNNFLAGLEGSSAILLGLVLGGMMAVDMGGPVNKA
                     AYVFGTGTLAATVADGGSVAMAAVMAGGMVPPLAVFVATLLFKDKFTQEERNSGLTNI
                     VMGLSFITEGAIPFGAADPARAIPSFIAGSALAGALVGLSGIQLMAPHGGIFVIALTS
                     NPLLYIAYVLIGAVVSGVLYGALRKAK"
     gene            complement(8808..9719)
                     /gene="pfkB"
                     /locus_tag="ACHU66_001177"
     CDS             complement(8808..9719)
                     /gene="pfkB"
                     /locus_tag="ACHU66_001177"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR3389465.1"
                     /translation="MIYTVTLNPAIDYIVRLEHVETGSVNRMESDDKYAGGKGINVSR
                     VLKRLGYPNTATGFLGGFTGQFIKDGLIAEGIDTDFVQVDQDTRINVKIKADQETEIN
                     GLGPIVTDTQLAELEMVLAGLTKEDTVVFAGSAPASLGNEVYNRLIPVAKKAGAQVVC
                     DFEGQTLLDSLAHQPLLVKPNNHELEAIFNVELNDIADIETYAKKILEMGAQNVIISM
                     AGDGALLVTPAATYFAKPIKGTVKNSVGAGDSMVAGFTGEYVRSQDPIEALKWGVACG
                     TATAFSDDLASIEFIKETYEKVEVETL"
     gene            complement(9716..10465)
                     /locus_tag="ACHU66_001178"
     CDS             complement(9716..10465)
                     /locus_tag="ACHU66_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR3389466.1"
                     /translation="MNILKSERKQIILERIHSQQFVRLEELIEVLDTSESTVRRDLDE
                     LENEGKLRRVHGGAEAPRNLQLEESILEKSVKNVQEKREIAERAAQQIVNGDVIFLDA
                     GTTTGVLIDYLQQENLTVVTNSIHHAVRLVERKIKTIIIGGFVKQSTDASVGAVALEQ
                     IRQLNFDKAFLGMNGIDKNYFTTPDIEEATIKRTIIDNAKESYVLADASKIGQFSFVK
                     VAAIEKSNIICQSSESSLLDIIKEKTRVIEV"
     gene            complement(10569..11624)
                     /locus_tag="ACHU66_001179"
     CDS             complement(10569..11624)
                     /locus_tag="ACHU66_001179"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR3389467.1"
                     /translation="MKRKVFEHYQPYKIKQKARFDLGNNENRIIDWSFLPQETLENLD
                     VNQLSFYGDNTYSELIEKYAAYLGVNPKQVTVGVGSDHLIHMIVSTFMEKDDTFLTVN
                     PDFFMYETYNHMHGSRFETIDLEAKNDTLVLPVEKLLARAEEVKAKIIMLSNPNNPSS
                     VAYSLEDLEKLAISFKGLLVVDEAYVEFANVESFISRLQQFDNVLVLRTLSKAFGLAG
                     LRVGFAVGSEELIYELDKVIPPFSLSNLTAKMAAVALNYTDKVLETVGVIQNLREEMI
                     LFLQGLDNCQVLPSQASFVTFKAPWAEEFYQQALAQDWNFKYYPTGKLEGHIRLSIGR
                     PEEMDMMKKMIEKLVEA"
     gene            complement(11655..12380)
                     /gene="trmD"
                     /locus_tag="ACHU66_001180"
     CDS             complement(11655..12380)
                     /gene="trmD"
                     /locus_tag="ACHU66_001180"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR3389468.1"
                     /translation="MRIDILTLFPEMFAPLEHSIVGKARDKGLLEINYHNFREKAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDTMDSIEQAKPRVILLDPAGRTFNQAYAEELAQEEQ
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKEVSH
                     TDDSFSSGLLEYPQYTRPYEYRGMVVPDVLMSGHHENIRKWRLEESLRKTYQRRPDLL
                     ENYNFTAEELTIFEKIKAEDTVD"
     gene            complement(12370..12888)
                     /gene="rimM"
                     /locus_tag="ACHU66_001181"
     CDS             complement(12370..12888)
                     /gene="rimM"
                     /locus_tag="ACHU66_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR3389469.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAKLALFDDK
                     DQFVMEVEIANHRKAKNFDIIKFKGMYHINDIEKYKGFSLKIAEENLTDLEDGEFYYH
                     EIIGLDVYENDILIGQIKEILQPGANDVWVVKRKGKKDLLLPYIPPVVLNIDIPNNRV
                     DVELLEGLDDEN"
     gene            complement(12986..14335)
                     /locus_tag="ACHU66_001182"
     CDS             complement(12986..14335)
                     /locus_tag="ACHU66_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFR3389470.1"
                     /translation="MYQTRNNKEKLWLFIKIFLPILIYQFANYSASFIDTMMTGQYST
                     MDLAGVSMATSLWNPLFSFLTGIVSALVPIIAQYLGKGERDKIREEFHQFVYLAIGLT
                     ILLLCSVLFIAVPALAQFGLDESIFQVGRQYLYFISIGILPLLLFSVCRSFFDALGLT
                     QLSMYLMLLLVPFNSLFNYLLIYGKMGLPALGGAGAGLGTALAYWAVLLVIVIVMCKN
                     KTIASYQIWQWSPIDLSLLKEGLKIGLPIGLQVFAEVAIFAVVGLYMAKFSAQIIAAH
                     QSAMNFATLLYAFPASVSSALAIVIAYEVGAKRPQDVKAYSRLGRLVALGFAVLTLTF
                     LYFFRSKVAYLYGNDAEFIRLTSHFLSFALMFQLADAFTAPIQGILRGYKDTTVPFIL
                     GLVAYWSMTFPVAFLLERFFQMGPEAYWIGLISGIFVCGIALNLRLIIIAHSHLSVK"
     gene            complement(14405..15388)
                     /gene="guaC"
                     /locus_tag="ACHU66_001183"
     CDS             complement(14405..15388)
                     /gene="guaC"
                     /locus_tag="ACHU66_001183"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR3389471.1"
                     /translation="MFNETPVFDYEDIQLIPNKCIINSRSEADTTVTLGKYSFKLPVV
                     PANMQTIIDEDVAEMLAKDGYFYIMHRFDEAGRIPFIKRMHEQGLIASISVGVKEYEY
                     EFVTSLKADAPEFITIDIAHGHAESVIKMIQHIKKELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHIESPGKTIEVDGEKFKEYYGSASEYQKGAYKNVEGKKIL
                     LPAKGHLKDTLVEMEQDLQSSISYAGGRDITSLKHVDYVIVKNSIWNGDSI"
BASE COUNT         4530 a         3591 c         2947 g         4445 t
ORIGIN      
        1 agaattagag tccccagtag agggagaatc aacatggttg ctacgtttcc ccagctagac
       61 gggaaaaacc agtcacgaat ccagttatca aggaaaaaag cagataacaa aatcaataaa
      121 ccaagaatgg tcagatagtt gattatttta gacatgttca cctgtttcat aaggtgacac
      181 tcctttctct ttacactttc attgtacgct taaatactac atttttcaat atatgtatat
      241 aaagaaaatg tgacagcgga attgaaaaaa agcagagttt cccctgcttt taacgattat
      301 ttcaagacaa gtgatgctgc gcccaataca cctgcatcat taccacgtgt tgccaagaca
      361 atttgcgtgc tttccttgat ttgtgggaag ctattttcag caaagacttt acgaacacca
      421 tccaataaga gctctcctgc agctgataca ccgccaccca gaacgatgta ggcaggattg
      481 aggactgcag caatgtttgc acaagcaaca cctaggtatt ctgagaagtg acggtagaca
      541 atcaaagcca aatcatcgcc ttcttttgcc aaatcaaaga catctttagc cgttacatct
      601 tgcccatcat caatcatttg tttcagtttt gcatcgcctg cgtattggtc cgcataacga
      661 cggctaaggt tgacaatacc tgtcgctgaa gccacggttt ccaagcaacc tttcttacca
      721 caagtacatg caaatggctc atcaaagtca actgtgatat ggcccaattc accaccagcg
      781 cctttgaccc cacgaatcaa gtttccagca gcgatgacac cgccaccgac acctgtacca
      841 agcgtcataa agacaacgtt tggattgttg tttccagcac caacccactg ctcaccgaga
      901 gccgctacgt ttgcatcatt gtcgatgaaa aatggtaaac cgagcgcaga ttcaaactga
      961 tctttaacca attgcaaggt cttccagttg aggttatagg ctccgataac agtgccagct
     1021 tcactatcca ctacaccggg tgagcccata ccaattccta agaaattgtc ctttgtcaaa
     1081 ccatgggttt caaaacgatg ttgaatgctg tcaataatat ctggaacaat atggcttcca
     1141 tcgtctaata tatttgtctt gatagaccat ttttcttgaa tttctccctc agtagtcaaa
     1201 atggccaatt taacagaagt tcccccaagg tcaataccga taattttttt agacataatc
     1261 atcacctcaa atgtattttc tttattataa cacaatgcaa aggtttgcgt aaagcctttg
     1321 caaaaatttg tacggataaa aattaattgc tctatcgaaa gagatagagc aaaattaaaa
     1381 attgaatatg taagataaac cttccaatgg aaaagagcat aactgtcgta aaattactac
     1441 cttctttcgt tccattatag agaaaaagag tagaaataaa ggtaaatcct agagcaaacc
     1501 ccaccatctg caagtcgtga taagactgac tataaaaata caaggtgctt gtcagcaata
     1561 tcatggatag gatagttaaa atcaagcgca atgtattcat ttttttggtc tttagatagc
     1621 taaacaaagc cgctcccaag gtcaccaaga gatagatgac catatatgac atgacaagaa
     1681 ctttcatcta gccctccagt tcgctcagat actcataccg ctcatatttt tccaagagca
     1741 gatcgttttt ctcatccaat tccttctgca aatcggataa ttttgtaaaa tcactaccac
     1801 aggtcagcat ttccgcctcg attgccgcaa tccgctcctc caagccagca atatcctctt
     1861 cgatggttgc ccattcctgc ttctcaaagt aggacatccg cttcttgttc tctttgattt
     1921 tctcggtttt ctcctttggt ttttccaaag aaatggcaga gctggaagcc agaaaggcct
     1981 tctcatccag atagtctgtg tagttaccaa agaaggtctc tataccgcta tcttcgaaag
     2041 ccaagatttt attggctacc ttgtctagga aataacggtc gtgactaacg gtaatgactg
     2101 gaccagtaaa gctttgcaag aaatgttcca agactgtcag agtcgctatg tccaagtcgt
     2161 tggtcggctc atctagtaag agaacattgg gccgttgcaa gagaatcttc agcaaataaa
     2221 gtcgcttctt ctccccacca gacaatttct caatcagggt accatgggta ttacgtggga
     2281 agaggaattg ctctaagagt tcggcaattg aaaccatacc agaagtcgtc ttagcttctt
     2341 ctgccacttc ctgcaagaaa ttgatgaccc gcttgctttc gtccaagccc ttgatggtct
     2401 gagaaaaata accgatacgg atggtttcac caatatctac cttaccgctg tctgcctgca
     2461 aatcacctgc gataagattg agtaaggtag attttccttt tccattatca ccgacaattc
     2521 ctatgcggtc cttgttttga atgagcaggt taaagtctgc taaaatgggc ttatcaggat
     2581 aggaaaagct gacattttca aagttaatga cttttttacc gatacgagag gtttcaaaat
     2641 tgatttccaa ttcgctatcg tctatcttgt tggctaaatc gcctttaagg tcatggaaac
     2701 gattgatacg ggcctgttgc ttggtagcac gagcttgagg ttgtctgcgc atccaagcca
     2761 actcttgttt gtagagctgc tctttcttat ggcgaagggc ggcatctcgc tcgtcctgtt
     2821 ctgcctttaa gcgaacatag tcctggtaat ttccctgata ttcggttaag cttgcgcgat
     2881 ccaattcaaa aatccgtgtc gccacattgt ccaagaaata gcgatcgtgg gtaataaaga
     2941 gaacagactt tttagtattt ttcaagaaag tcgtcaacca ctcaatggta tcaatgtcca
     3001 ggtggttagt cggttcatcc agcaagagca agtcagcatt gccaaggagg acttgggcca
     3061 attgcactcg tctacgtaga ccacctgata actcaacaac ggttttgttg aggtctgtca
     3121 gaccgagttt tgacagaaca gtcttgacct gactttcaat ctcccaagca ttgagagaat
     3181 ccatctctgc catgactttc tccagtcttg cttgacttgc ttcatcgtaa tgagataaga
     3241 gcaactcgta ttcacgaatc agctgggttt cgcgtaggtc agatgaaaga acggtgtcta
     3301 aaactgtctt actttcatca aaatcaggtt cctgggtcag ataggcgatt ttataggcat
     3361 tttttgtacg aaaaggtgat acatctccgt caaaaccgat acgacctgac aggacatcca
     3421 acaaagtcgt ctttcctgta ccattgaccc caataatccc gattctgtcg ccttgatgga
     3481 tgatgaaaga cagatcagca aagaccgttt tatctccgac agatttagtc aggtgttcaa
     3541 cgataaaatc actcattctc taagattcct ttcacaaatg tcataatcgc ttgctcttcg
     3601 ttttccagct gtccttccac aatctggaat tccacctctt tgagtagttc tcccaatact
     3661 ggacctggtt tgaggtcaaa tgccttcatc agatgtcctc cattgacaac aatttcatgc
     3721 ttgtcgtgga tagtcaaggc ttggtcaatc ttatcgattg ctacaaaatc tgtgacaaga
     3781 ccttgtgcct gacgaagttc ctctaccaag tataagaatt ctttgccata cttgaaacag
     3841 atttgtttag tgacaggtcc tgcttggcgg agctgataaa tctctactaa tttgaccacg
     3901 ctacgttgga aatcattgct ggttttccat tttttcagga aggatttgat gctatcaata
     3961 cctaggcaga caaagagcat ggcccaagct tgttcagatg tcgaaaagcg aaactcttct
     4021 gtcagacttg ttagcatggt ctctaattca tcactttttc cagctaaatc aggcaagaaa
     4081 ttgtaagcct tggaatcaat catagcgcgc aagccttttc gccaaaaatc cgccatcaag
     4141 agtttatcaa actcgataaa gctacgttcc accgagattt tttctaaaag cggtgcagtt
     4201 ttaaccatgg cttcaaaggt agtcggctca atttcaaagt ccaaggtcgc tgcaaagcga
     4261 aatccacgca tgatacggag agcatcttcg ttgaaacgct cagccgggat gcctacagcc
     4321 cgtagggtgc ggttttccaa gtcagtcatc ccttcaaaaa ggtcaatgat ttgagcctct
     4381 tcatcaaggg caaaggcatt aactgtgaag tcgcgacgtt tgagatcctc ttctaaggaa
     4441 cgcacaaaag aaacctgact cggcctacgg aagtcgacat actcctcttc tgtccgaaag
     4501 gtggtaattt catactcctt tttcccctct aataccaaca ccgtaccgtg ttcaattccg
     4561 acatcaatag tccgtgaaaa gatttgtttc gtttcctgag ggtagctgga tgtcgcaata
     4621 tcgacatcat gaataggcct accgagaatg gcatcacgaa cggaaccgcc aacgaaataa
     4681 gcctcgtaac cagctgcttt aattttctct aaaatcggca aagcctcctg aaactcagaa
     4741 ggcagattgt ttaatttcat aataagtgtt ccaaaccata aacgagttgt gagcgtttga
     4801 ctacctcttt aatgccgaga ttgacaccgc ccataaagga aatacgatcg taagagtcat
     4861 gacgaatggt caagccttct ccctgtgccc caaaaatcac ttcctgatgg gcaacaaggc
     4921 ctggcaagcg tactgagtgg atacggaagc catcaaatgc tgcaccgcga gcgccagcaa
     4981 tcagttcttc ttcatccgct gcaccttgag tctgtgactg acggacttgt gaaatgagtt
     5041 cggcagtttt gacagccgta ccactcggtg catccttctt cttatcgtgg tgtaattcaa
     5101 tgatttcaag atttgggaaa tacttggctg cctgtgctgc aaattgcatg agcaagatag
     5161 cgccaatagc aaagttagga gcaatcaagc cgcccaaacc cttctcagca gataaggcgg
     5221 tcaattcctc gatttcttca ggggtaaatc ctgtcgttcc aaccacaggt gcgaaaccat
     5281 tttccaaggc aaaacgggtg ttttcatagg caaattttgg cgttgtaaaa tccacccaga
     5341 catcagcttc taggctggca acttcttctt tggtcttgaa aacaggaaca ccatctactt
     5401 ctgtttctgt cgcaaaggga tccaccaagg cagccaaact aagtgctgcg tcacctttga
     5461 ccatctcaac agccgttgag cccatttttc ctttgaatcc tgcgataatt accttaattg
     5521 tcatgcaaac tcctattcaa taatgggaga aataccaaag gccactgccc cttctcccaa
     5581 gtgcgtaccg atgaccgaac caaatgacac gattggtaaa tcgctaccga cacctgcttc
     5641 ttccaactgc tgtttaaagt tttcagcctt ctgaggtgcg ttggcgtgta tgatagcaat
     5701 ctgatactgt ccttcggtcg tttgttcttg gaggatttcc aagaggcgtt taatggcctt
     5761 tttctccgtc cgaacttttt catacacttc aatcttaccc tcgcctgtaa aatacagaat
     5821 cggtttgatg ctcaaaagat tgccaagcaa ggctgcacca ttggacaagc gaccaccttt
     5881 taccaaatga ttgaggtcat caaccatgat gaaggctgta gtcttcccaa tttcttggtc
     5941 caatttccca gtaatctctt caaaactaag tcctgcatca gcccatttga gaacattttc
     6001 gaccatcatg cctaaaggag ccgatgtaat tttggtatct ggaaaggcaa cggttaatcc
     6061 ttcaaattca tcagccaaat attgaatatt ttgataaaaa cctgaaatac ctgatgacaa
     6121 gaataagcca agtgcgtgtg tatagccttt ctctgttaat ttagccagaa tttcttctag
     6181 ttccatcaaa ctcggttgac ttgttttagg taattctttc gatgaagcca ttttttgata
     6241 aaactcttca accgtcaaat tttgcccttc tatataattt tccccatcga tgctgactgg
     6301 aatacttagg acaaacaggt catcccgctc aatactttga acattcaaaa tagccgatga
     6361 gtcagttata actgctaact tcatattaaa actccaaatt cactcctggt gcatcaaagg
     6421 cgatttcagt aacctcatag gtcattcgtt ttaaaatgtc caaaagaggc tctaccaaaa
     6481 cacgtttttc ctcatcagta aattcagttg gctcttctac caatcgattt ggtaagtgaa
     6541 cggcctgact catggcacct gaaatcacaa aatgatctag tacaatcata aagcgcaaaa
     6601 tggtaatcat cgatgttcga ttttccgcac gattttgctc aatcaattga aagtcaactt
     6661 tcaattttgt ttccggaatt ccgttctctt tttcccacgc gtgattgcgg gcatcaaaat
     6721 gatactgatt gacaaattct ttatcgcgaa tgatttccat ttcaagactc ctctaataac
     6781 aataatactc acattatacc ataatcctcg tgaaatagct aaaaaacctt gccagaagca
     6841 aggtttcaat caaaattatt tagctttacg aagtgctccg taaaggacac ctgatacaac
     6901 cgcaccgatc aaaacatagg cgatgtaaag caatggatta gatgtcaaag cgataacgaa
     6961 gataccaccg tgaggagcca tcaattggat gcctgacaaa ccaaccaagg cacctgcaag
     7021 ggcagaacca gcgataaagc ttgggattgc acgagctggg tcagcagcac caaatggaat
     7081 cgcaccttca gtgatgaatg aaagacccat aacgatgttt gtcaaacctg agttacgctc
     7141 ttcctgtgtg aatttgtctt tgaaaagaag ggttgcaacg aatactgcca acggtggaac
     7201 cataccacct gccattaccg ctgccatggc aacagaacca ccatctgcaa cagttgctgc
     7261 aagtgtacct gtaccgaaga cataagctgc cttgttgact ggaccaccca tatcgactgc
     7321 catcattcca ccgaggacaa gtccaaggag aatagcagag ctaccttcaa gacctgcaag
     7381 gaagttattc atgcctgtgt tgatagctgc cattgggatg ttaacgaaga acatcaagaa
     7441 accagtgatt gctgttccca aaagtggcaa gagcaagata gcgttcaaac cttgaagtga
     7501 ttttggaagg ttgcgcaaag cgttgcgaag aacgagcact acaccaccag caaggaaacc
     7561 accaacaagg gcacccaaga aaccagatga tacacctgca agagcaagcg tttcttcacc
     7621 accagctgcg taaggaattt taccgaacgc aagaccgcta ctagcaattg caccagctac
     7681 gaaacctgca accaaacctg gtttttcagc gattgagtaa gcaatgtaac ctgctagaag
     7741 tggcaacatg aagccgaagg ctgctccacc gatcttcatg aacatagctg cgatttcatg
     7801 gtatgaacca agactaccaa ggctgtcttg tggtacacct aaagctccat cgaataggaa
     7861 ggcaagggca atcaagatac cgccaccgat aacgaatggc aacatttgag aaacaccact
     7921 catcaagtgt ttatagaacg ctttgccaag actgagtttt tctgaagatt ccacagtagc
     7981 tgctccttct gacgctgtgt aggctgctgc tttaccgttt aagatgatgt taatcaattc
     8041 ttctggttgt ttgataccag ctgcaactgg acgagatacc aatggtttgc cattgaaacg
     8101 aggcatgtca acagccttat ctgctgcgat gataacaccc gccgcattct tgatgtcttc
     8161 agcagtcagt ttgttaccaa ccccagacgc accgtttgtt tcaaccttga tgccaacacc
     8221 catctcagca gcctgcttct taagagcttc ttctgccata taagtgtgag caataccagt
     8281 tgtacatgct gtaactgcaa cgataaatgg acggtcgcca ctaggagctg caaccacttc
     8341 ttctactgcc ttgtcagctg cttcagctgt atcgaagact gcaatcactt cttcaggatt
     8401 tgttgccgca cgaagtctat cagcaaatcc agctttcatc aagtatttag acaattccgc
     8461 aagggctgcc aagtgcgtgt cgttggcacc ttctggagct gcaatcatga agaacaagtc
     8521 agttggttga ccatcaagac ttgcataatc aacaccattg tttgattttg caaagagaac
     8581 agtcgcttct tttacagccg cattcttagc gtgtggcatt gcgataccgt caccaagtcc
     8641 tgttgtcgtt tgtgcttcac ggttcatgat acctgttttg aaaacatcaa aatccgttac
     8701 ataaccttta tcaaccaaac tagcaatcat ttcatcaata actgcttcct tgcttgtcgc
     8761 ttgcaaatca agcaacatga catcctttct caaaacgtct tgaattttca tagtgtttct
     8821 acctcaactt tttcataagt ttccttaata aattcgatac ttgccaaatc atctgaaaag
     8881 gctgttgccg taccgcaagc aactccccat tttagggctt caattgggtc ttgtgagcga
     8941 acgtattcgc cagtgaatcc tgccaccatg gaatctccag ctccgacaga gtttttcact
     9001 gtacccttga ttggtttggc aaagtaagta gctgctggag tgaccaacaa agccccatcc
     9061 cctgccatgg aaatgataac attctgagca cccatttcta agattttctt agcgtaggtc
     9121 tcgatatctg caatgtcatt caactcgacg ttgaagattg cttcaagttc atggttgttt
     9181 ggcttgacca agagtggctg gtgtgctagt gagtctagaa gtgtttgacc ttcaaagtca
     9241 cagaccactt gcgctcctgc ttttttagcc acaggaatca gcctattata gacttcattt
     9301 ccaagactag ctggagcaga cccagcaaag acaacggtgt cttctttggt caatcctgct
     9361 aagaccattt ctaattcggc caactgtgta tctgtcacaa tcggacccaa accgttgatt
     9421 tctgtttctt ggtcagcttt gattttcacg ttaatacgtg tatcttgatc aacctgaaca
     9481 aaatcagtat caattccttc ggcaatcaat ccatctttga tgaactgacc tgtgaatccc
     9541 ccaaggaaac ccgttgctgt attaggatag cccaatcgct tcaaaacacg gctaacgtta
     9601 attcccttac cgccggcata cttatcgtcg ctctccatac gattgacact gcccgtctcg
     9661 acatgttcta aacgaacgat atagtcaatc gctggattga gcgtaactgt ataaatcata
     9721 cctcgattac cctcgttttc tcttttatga tgtccaacaa actgctttct gaagattggc
     9781 agataatgtt ggatttttcg attgccgcaa ctttaacaaa ggagaattga ccaattttag
     9841 aagcatctgc caaaacatag ctttctttag cattgtctat gatagtgcgt ttgatggtcg
     9901 cttcttcaat atctggcgtt gtaaaatagt ttttatctat accattcatc cccagaaatg
     9961 ccttatcaaa attgagctgt cgaatttgtt ccaatgccac tgctccaacg gaagcatctg
    10021 ttgattgctt aacaaaacca cctataatga ttgtttttat cttgcgttca accagcctca
    10081 ctgcgtggtg aatagaattg gtaacgactg tcagattttc ctgctgaaga taatcaatca
    10141 gtacaccggt tgttgtacct gcatctaaaa aaattacgtc tccgttaaca atttgttgag
    10201 cagctctttc cgcaatctct cgtttttctt gaacgttttt gacagatttt tctagaatag
    10261 actcttcgag ttgcaaattc cttggggctt cagcaccgcc gtgtacacga cgaagcttac
    10321 cttcgttttc caactcgtcc aaatccctac gtacagtaga ctcagatgta tcaagaactt
    10381 cgattaattc ctctagccga acaaactgtt gagagtgtat tcgctccaat ataatctgtt
    10441 ttcgttctga tttgagtatg ttcaacacct cctgcaatcg tttacaaaaa tcattataca
    10501 cgattttctg ccattgtcaa tcattttcta tcattttctt tcaaaaaaac aaaaaaacta
    10561 gcagaaatct acgcttccac tagtttctca atcatttttt tcatcatatc catttcttct
    10621 ggacgaccaa tagataggcg aatatgcccc tctaatttac ctgttggata atatttgaaa
    10681 ttccaatcct gtgccaaggc ttgctgataa aattcttctg cccatggtgc tttaaacgtt
    10741 acaaaactag cctgactagg tagaacttga cagttatcta acccttgcaa gaataaaatc
    10801 atttcttctc gcaaattctg aattactccc actgtttcta aaaccttgtc tgtataattc
    10861 aatgcaacgg ctgccatttt tgcagtcaag tttgacaagc tgaatggggg aatgaccttg
    10921 tccaattcat aaattaactc ttcgcttcct accgcaaagc caactcgtaa acctgcaagt
    10981 ccaaaagcct ttgataaggt ccgtaaaact aaaacattgt caaattgttg cagacgactg
    11041 atgaagcttt cgacatttgc aaattcaaca taagcctcat ctacaacgag tagcccttta
    11101 aaagaaatag ccaatttctc caaatcttct agtgaataag ctactgaaga aggattgtta
    11161 ggattggaaa gcatgattat ctttgccttc acctcctctg cacgcgccaa caatttctct
    11221 actggcagga cgagcgtatc gttttttgct tccaaatcaa tggtttcaaa acggctacca
    11281 tgcatgtgat tataggtttc atacatgaag aagtctggat tgacagtcaa aaaagtatca
    11341 tccttttcca taaaggtgga aacaatcatg tgaataaggt gatcagagcc cacacctact
    11401 gttacttgtt tgggattcac tccaagatag gccgcatatt tctcgatcag ttcggagtaa
    11461 gtgttatcac cataaaaact taattgattg acatccaaat tttccaaggt ctcttgtggt
    11521 aaaaaggacc aatcaataat gcgattttca ttatttccca agtcaaacct agccttttgt
    11581 ttgattttat aaggttggta atgttcaaat accttgcgtt tcatcatttc tccttctagc
    11641 aagaaaaaca cagtctaatc aactgtgtct tctgccttga ttttttcaaa aatagttaac
    11701 tcttcagctg taaagttgta gttttccaac aagtctggac gacgttgata ggttttgcgt
    11761 agactttctt ccaaacgcca tttacgaata ttttcatggt gaccgctcat taatacatct
    11821 ggaactacca ttccacgata ctcgtatggt cgggtgtatt gaggatattc cagtagacca
    11881 gatgaaaaac tatcatccgt atgactgact tctttgccaa taacctctgg aatcagacga
    11941 acggtagcat caatcatggt catagctgcc aactctccac ctgtcagaac atagtctccg
    12001 agagaaatct catctgttac caaggtctta atccgctcat catatccttc atagtgaccg
    12061 caaatgaaaa tcagctgctc ttcctgtgcc aattcttcgg cataggcctg attgaaagta
    12121 cgcccagcgg gatccaatag gataacacgt ggcttggctt gttcaatgct atccatggta
    12181 tcaaaaattg gctgggcacg gagcaacatg ccttgtcctc caccataggg ttcatcgtcc
    12241 acatggcgag ccttttcagc tttctctcgg aaattgtggt agttgatttc tagcaagccc
    12301 ttgtcacgcg ccttacccac aatagagtgt tctagaggtg caaacatctc agggaagagg
    12361 gtcaaaatat caattctcat cgtctaggcc ctcgagcaat tccacatcga cacggttatt
    12421 tggaatatca atgttcaaga caacaggtgg aatatatggc aagagcaggt ctttcttacc
    12481 ttttcgtttc accacccaga catcattggc acctggctgc aagatttcct tgatttgtcc
    12541 gatcagaata tcattctcat agacatccaa gccgataatt tcatggtaat aaaactcacc
    12601 atcttcaagg tcagtcaagt tttcctcggc aattttcaaa ctaaagccct tgtacttttc
    12661 aatatcgttg atatggtaca tgcccttaaa cttgataata tcaaagttct tagccttacg
    12721 gtgattagca atttccactt ccatgacaaa ctgatccttg tcatcaaaaa gtgcaagctt
    12781 tgcccctttt ttaaaacgtt cttctgcaaa atcggtcaca gacaaaacac gcatttcccc
    12841 ttgtaggcct tgcgtgttaa caatctttcc tacgttaaaa taattcataa tttctccatt
    12901 tgattttcta tccttttatt ttaacatgag caagatattt tcacaagaga gaaaaagccg
    12961 gatgtctccg actttcatag ttaatttatt tcacggacaa atgagaatga gctattatga
    13021 tcaagcgcaa attaagagct attccgcaga caaaaatccc tgaaataaga ccgatccaat
    13081 aggcttctgg acccatttgg aaaaatcttt caagcaagaa tgcgactggg aaggtcatgg
    13141 accaataagc aaccaaacca agtataaatg gaacagtcgt atccttgtac ccacgtaaaa
    13201 tcccctgaat aggtgctgta aaggcatctg ctaactggaa catgagggca aagctcagga
    13261 aatgcgaggt caaacggata aattctgcgt cattaccata aaggtaggca actttggagc
    13321 ggaaaaaata taggaaagtg agtgttagga ctgcaaaacc aagtgcaacc aagcgtccta
    13381 gacggctgta ggctttcaca tcctgtggtc gtttagcccc tacttcataa gcaatgacaa
    13441 tagctagggc tgaagaaact gaggcaggaa aggcatataa taatgtcgca aagttcatag
    13501 ctgactggtg ggctgctata atctgtgctg aaaattttgc catgtagagc cctactactg
    13561 caaaaattgc tacttctgca aagacttgta aaccgatggg aagaccgatt ttcaaacctt
    13621 ccttcaataa agaaaggtca atcggactcc actgccaaat ctgataagat gcaatagtct
    13681 tatttttgca catgacaatg acgattacta gcaataccgc ccagtaagct aaagcagtac
    13741 ccaatccagc tccagctccg cccagggccg gtaggcccat tttcccataa attaagagat
    13801 agttaaatag actgttaaat gggacaagga gcaacatgag gtacatggat agctgagtca
    13861 aaccgagagc gtcgaaaaaa gagcgacaga cactaaagag caagagaggc aggatgccaa
    13921 tggaaataaa atagaggtac tgccgaccaa cttgaaaaat agattcatca agtccaaact
    13981 gagccaaggc gggaacagcg ataaatagga ccgagcacaa gagtaaaatg gttaatccga
    14041 tggctagata gacaaactga tgaaattcct cacgaatctt atctctttca ccttttccta
    14101 aatattgagc gatgattggg accaacgcag atacaatccc tgttagaaat gaaaaaagcg
    14161 gattccaaag acttgttgcc atggaaacac ctgccaaatc catggtacta tactgaccag
    14221 tcatcatagt atcaatgaag gaggctgaat aattagcaaa ttgataaatt aaaataggca
    14281 aaaatatttt gataaagagc cagagcttct ctttgttatt tcttgtttgg tacatactta
    14341 ttctctttca attttatata aatattgtaa caagaaaagg acctgcattg caagtcctct
    14401 ttgactagat agagtcacca ttccagatag agtttttcac gataacatag tccacatgct
    14461 tcaagctggt aatgtcacgt cctcctgcat aagaaataga tgactgcaaa tcttgctcca
    14521 tttcaaccag agtatctttc aaatgtcctt tggcagggag taagattttc ttaccttcta
    14581 cgtttttata ggcacctttt tggtattcag atgcagaacc gtagtactct ttgaatttct
    14641 caccgtcaac ttcaattgtt ttacctgggc tttcaatgtg accggcaaag agcgaaccaa
    14701 tcatgaccat gcttgcaccg aagcggatgg atttggcaat atctccgtgg gtacggatac
    14761 ctccgtccgc aataattggt ttacgggcag cttttgcaca ccaacgaagg gctgccaact
    14821 gccaaccacc tgtaccaaaa ccagtcttaa cctttgtaat acatacttta cctggaccaa
    14881 taccaacttt tgtcgcatct gcaccagcat tttctagttc acgaactgct tctggagtac
    14941 cgacattacc tgcaatgacg aacgtctcag gcagttcttt cttgatatgt tggatcatct
    15001 tgataacact ttctgcatgg ccatgagcaa tatcaatggt gataaactca ggggcatcag
    15061 ctttcaagct agtcacaaac tcatattcat attcttttac gccaacagag atagaagcaa
    15121 tcaaaccttg ctcgtgcatg cgtttgataa atggaatacg accagcttca tcgaaacgat
    15181 gcatgatgta gaaataacca tctttggcaa gcatttctgc cacatcttca tcaataatag
    15241 tctgcatatt agctggaaca actggtaatt taaagctata tttcccaaga gtcactgttg
    15301 tatctgcctc tgaacggcta ttgatgatac atttatttgg aatgagctga atatcttcgt
    15361 agtcaaaaac tggagtttcg ttgaacatgc ttctacctct ttttcttaac gttggaacag
    15421 aaaaacggtt tgtttttcgt attttttcct tattacaaga tatagtatag cttattgttt
    15481 gtgttttgtc aataaaaaag aaacctatct aga
//
LOCUS       DAZQCJ010000042        14979 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000042 DAZQCJ010000000
VERSION     DAZQCJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14979
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.043"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(30..971)
                     /locus_tag="ACHU66_001184"
     CDS             complement(30..971)
                     /locus_tag="ACHU66_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFR3389472.1"
                     /translation="MLVALDEDGQVFNVLENPAPQGRYSCPGCGGLVRYKSGKVLRSH
                     FAHVTLRDCHYFSENESAQHLSLKSCLYSWLINAEQVELEKCLPSIGQVADLFVNNSL
                     ALEVQCSSLPISRLQLRTQAYHEAGLQVLWLLGKGLWIKERLSKLHKQFLSFSMNMGF
                     HLWELDDEKKELRLRYLIHEDLRGKVHCLTKVFPFGEGNLLDILRLPFAKQALSHLTF
                     PLDRDLPRYIAQQLYYRSPNWLTLQAESYSRGENLLTKTAEEWYPHIRLPRSAIGFAQ
                     IQKDLTLVYQDFDQYYGNIEDKQKQVLYPPIIYRKPM"
     gene            complement(1035..3092)
                     /gene="metG"
                     /locus_tag="ACHU66_001185"
     CDS             complement(1035..3092)
                     /gene="metG"
                     /locus_tag="ACHU66_001185"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR3389473.1"
                     /translation="MGCYQEQGFICPELKGKSMTKTPFYITTPIYYPSGKLHIGSAYT
                     TIACDVLARYKRLMGHEVFYLTGLDEHGQKIEEKAKEAGLTPQAYVDGMAVGVKELWN
                     LLDISYDKFIRTTDDYHEEVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLEE
                     VFRDENGKVTGGIAPSGHEVAWVSEESYFLRLSKYQDKLVEFFNAHPDFIQPDGRMNE
                     IMKNFIEPGLEDLAVSRTSFTWGVPVPSNPKHVVYVWIDALLNYATALGYGQEETINY
                     DKFWNGTVYHMVGKDILRFHSIYWPIMLMMLDMKLPDLLVAHGWFVMKDGKMSKSKGN
                     VVYPEMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVA
                     MINKYFSGQVPTFVANVTDFDADLAAVVADNLASYHKYMEAVDYPRALEAVWNIISRT
                     NKYIDETAPWVLAKDEADRDKLAAVMAHLTAGLRVVAHLIQPFMMTTSNAIMEQLGLG
                     TAFDLENLDFAGLPAGLTVVAKGTPIFPRLDMEEEIAYIQAQMGGSSAISQEEEKEWN
                     PADVELKNEKAAIKFEDFDKVEIRVAEVKEVAKVEGSDKLLKFRLDAGDGEDRQILSG
                     IAKFYPNEQELVGKKVQIVANLKPRKMMGLLSQGMILSAEHGDNLILLTVDPSVPNGS
                     QIG"
     gene            3192..3785
                     /locus_tag="ACHU66_001186"
     CDS             3192..3785
                     /locus_tag="ACHU66_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HFR3389474.1"
                     /translation="MFTKKNILTHLFFLIIAVGLKRYGVTAPDLTWNMFLALVALDFA
                     ILTNHTKSHILKVIAGLLWLFFYPNTFYMVTDIVHMHFANTVLWQRESMILFMLYVPS
                     IFFGVMAGVESVRLIFSAFSIKSYWLKLFMISGLSLLSSFAIHIGRYARLNSWDIFTR
                     PTVVIDEMLAVISWDALPFILGFTFIQIMVLVFSINE"
     gene            4086..5123
                     /locus_tag="ACHU66_001187"
     CDS             4086..5123
                     /locus_tag="ACHU66_001187"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFR3389475.1"
                     /translation="MKEGDLLFTPISEKIDINQVREHSKLSPETLAKKQARDRELEAI
                     LKGEDNRLLLVIGPCSSDNEEAVLDYAHRLAKLQEEVKDKIFMVMRVYTAKPRTNGDG
                     YKGLMHQPDTDAAPSLINGIKAVRNLHYRVITETGLTTADEMLYPENLPLVDDLVSYI
                     AIGARSVENQQHRFVASGIDVPTGLKNPTSGNLKVMFNGLFAAQKKQSFLFNNTEVET
                     SGNPLTHVILRGAVNENGKYLPNYYYDNLLETIDLYDQFGLKNPFIIIDTNHDNSGKN
                     YLEQIRIVRQTLINRDWNESIRNYVRGFMIESYIEDGRQDNPDVYGKSITDPCLGWEK
                     TQELIREIYNR"
     gene            5133..6158
                     /locus_tag="ACHU66_001188"
     CDS             5133..6158
                     /locus_tag="ACHU66_001188"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFR3389476.1"
                     /translation="MGIHKRSTSLDIDKVKELSKLEGEFLAAKNRRDEELKRIIRGED
                     DRLLLVIGPCSSDNEEAVLEYARRLSKLQEEVKDKIFMVMRVYTAKPRTNGEGYKGLV
                     HQPDTSKLPDLINGIAAVRNLHYRVITETGLTTADEMLYSANYPLVEDLVSYHAIGAR
                     SVEDQEHRFVASGVDMPTGMKNPTSGNLTVMFNGIYAAQNKQNFIYRDAEVDTDGNPL
                     AHAILRGANTEKGGYEPNYYYDILLKTIKQYEQFGLKNPFIVIDTNHDNSGKTYLEQI
                     RIVRQTLINRAWNESIRKYVRGFMIESYLEDGRQDSPEVFGKSITDPCLGWKKTEALI
                     REIHQTV"
     gene            6204..7073
                     /locus_tag="ACHU66_001189"
     CDS             6204..7073
                     /locus_tag="ACHU66_001189"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR3389477.1"
                     /translation="MNIDGYTRLAAVVAKPIKHSISPFIHNLAFKETGVNGVYVAWEI
                     PEEDLAVTLENIKRYDMFGINLSMPYKQAVIPYLDDLTASARLIGAVNTVIHKDGKLI
                     GHNTDGIGFFKSLERLKGFQVKNKRLTILGGGGASTAIIAQATLDGAKEITIFCRQQS
                     LERTQASLTPIAQATGVPMQVLALDDSQLLQEHITNSDLLVNGTSVGMDGYSQPVPST
                     IRFPENLLVADVIYQPFETPLLKLAQSQGNPTINGLGMLLFQAAEAFQAWTGKEMPTD
                     LIWDQLVQKYDIK"
     gene            7086..8153
                     /gene="aroB"
                     /locus_tag="ACHU66_001190"
     CDS             7086..8153
                     /gene="aroB"
                     /locus_tag="ACHU66_001190"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR3389478.1"
                     /translation="MKLTVNLPNTPYDILIQRGSLAQTGTWIKELWKPQKIAIITDDH
                     VGSLYRETVQSSLEQAGFETIVFEFPEGEASKNLDTVNQAYEFLVKNGMTRSDGIIAL
                     GGGVVGDLAGFVASTYMRGIHFLQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFCQP
                     DGVLIDPDTLKTLGKRELIEGMGEVVKYGLIDDVELWETLDQLDGSVESILEHADYII
                     YHSCEVKRKVVVEDELDNGVRLYLNFGHTIGHAIEVTAGYGQVMHGEAVAIGMVQISR
                     AAEKKGLMPAGMTEKIIAMCEKFGLPTTHQPWNVDELYATLTHDKKARGKTIKLVIVP
                     QLGQAAIHQIPMEEMLEFLQV"
     gene            complement(8357..9157)
                     /locus_tag="ACHU66_001191"
     CDS             complement(8357..9157)
                     /locus_tag="ACHU66_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3389479.1"
                     /translation="MSTILSIQNIHKTFEAGTVNENHVLRGLNLDVKEGDFISIIGGN
                     GAGKSTFMNSLAGALTVDSGDILLEGKSIKHVSAAKRSKDISRVFQDPKMGTASRLTI
                     EENMAIAYRRGLSRGLGWGVKDSERAILKEALKELGLGLENRMKVDTQFLSGGQRQAL
                     TLMMASLVKPKVLLLDEHTAALDPKNSDMVMELTKKIVESHQLTALMITHNMENAIEY
                     GNRLVMLHRGQIVVDVEGEEKKNLTVQNLMDLFYKNSGEKLTDDEMIL"
     gene            complement(9167..10060)
                     /locus_tag="ACHU66_001192"
     CDS             complement(9167..10060)
                     /locus_tag="ACHU66_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR3389480.1"
                     /translation="MDIILSSISQGLLWSVMAIGVYLTFRILDIADMTAEGSYPLGAA
                     VCATGIVNGVNPLLATFMAMVAGMGAGLISGLLHTKLKIPALLTGIVTLTGLYSINLK
                     ILGKANVALLKQETLVTQLQDFGLTKTNAVLVIGLVFVLAVVGLLTLLLNTQIGLAIR
                     STGDNIPMSEANGINVDNMKIYGYMLSNGLIALCGALLTQNNGYADLNSGTGTIVIGL
                     ASVIIAEVILRNLRLGWRLLSVVLGAVVYRLIILAILEIPSMDADLVKLFSAILLATV
                     LYVPELQKKLNIRRPKLNGNA"
     gene            complement(10115..11089)
                     /locus_tag="ACHU66_001193"
     CDS             complement(10115..11089)
                     /locus_tag="ACHU66_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HFR3389481.1"
                     /translation="MYYKLVKKLATISVASMGLLTLAACSSSSEQASSDVVKVGVLQY
                     MEHESLTAAREGFVAELEANGYKEGEKLVLDYQNAQGDQANLQTISEQLIDGNDIVLA
                     IATPSAQSLATVSNETPIVFTAVTDPLSAGLVESIEKPGGLLTGTSDQAPIDKQVELL
                     GQAVPDAKTVGILYTTSERNSEVQVEQAKELLEKAGYKVVVKGITSSNEVQDATTSLM
                     KDVDALFIPTDNTVASTMTMIGELSVEHKVPVIGGSTDMVDEGGLLTYGTNYEALGRQ
                     TAKMAIKIIEGANVSETAVEYPETVSLHVNEEMAQKLGIDTSKLAVSE"
     gene            complement(11113..12072)
                     /locus_tag="ACHU66_001194"
     CDS             complement(11113..12072)
                     /locus_tag="ACHU66_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR3389482.1"
                     /translation="MGFKKIILSSVALLSAVTLAACSSNSSDSSSVNVGIIQYAEHEA
                     LSSAREGFVEVLEEAGYKEGENLTIDLQNAQGDQANLQTMVEKLAGKNDLNFAIATPA
                     AQAMLNADSETSSVFTAVTDPVEAGLVESLEKPGGTMTGSTDATDVEGQIEMLLKVVP
                     TAKTVGIFYNSSEVNSEVQAEQAKKALEAKGVKVEVTTVTTTNDVQQAITSLAGKVDA
                     IYLPTDNTVASTASTIGDILKEAKVPAMGSDAAVIDAALFTYGVDYHAIGVQAGELAV
                     KILKGEKPADLAVEKPNTAAITVNEEMAKAVGIDAATIEALEE"
     gene            complement(12813..13814)
                     /gene="lacD"
                     /locus_tag="ACHU66_001195"
     CDS             complement(12813..13814)
                     /gene="lacD"
                     /locus_tag="ACHU66_001195"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFR3389483.1"
                     /translation="MTKLQLSQAKFNHMTRLSNSDQVIAALAIDQRGALKRLLAAAAG
                     GGEFGDDILIDFKKVISSDLTPYASSILLDAEYGVPASELRHADCGFIAAYEKTGYDA
                     STPGRLPDLLPNWSAKRIKELGADAVKILLYYDVDDKPEINDIKHAWVERIGSECIAE
                     DIPYFVEILTYDASDMDVTSREYAALKPHKVNGAMREFSKSRYNADVLKVEVPVNMNF
                     VEGYTDEEPVYTVEEAKAFFKEQTDSTHLPFIYLSAGVSAKLFQETLVFAKEAGSSFN
                     GVLCGRATWKDAVAIFASEGEEAAKAWLADQGRRNVEELNEVLATTACPWTEKVEVK"
     gene            complement(13838..>14979)
                     /gene="nagA"
                     /locus_tag="ACHU66_001196"
     CDS             complement(13838..>14979)
                     /gene="nagA"
                     /locus_tag="ACHU66_001196"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR3389484.1"
                     /translation="VFIKAKSIILPESEVESAYLEITDKGQFGRIVTEKPEGEIVDYS
                     DFHLAPGLVDTHIHGYASHDVMDNDFEGIKVISEGLLSCGVTSWLPTTLTDTTENLDA
                     VCETIGTYAGQETGAKIQGIFLEGPFFTEKYKGAQNPKYMSDPSIEKLDNWNSLSKGL
                     VNKIAIAPEREGVKEFIEFANSKAIHTALAHSDATYTQAEAAVEAGANIFVHVYNGMS
                     GLHHREPGMVGAALNLKNVYAEMICDGHHVHPVAAEIVVKARGAEETVLITDCMRAGG
                     MGEGESRLGEFEVVVKDGTARLKESGSLAGSILELIEAVQNVVKWGLVSLPDALRMAS
                     LAPARSVNIDHICGRIAEGRAADFIVVDDAGRLQATYLDGVKRFG"
BASE COUNT         4344 a         3461 c         2883 g         4291 t
ORIGIN      
        1 atatatacac ctccatcagc tatctctatt tacattggtt ttctataaat tataggtgga
       61 tatagaacct gtttctgctt gtcttcaata ttgccgtaat actggtcaaa atcctgataa
      121 actaaggtta aatctttttg tatttgggca aagccaatag cagagcgggg tagacggata
      181 tgtgggtacc attcttctgc cgtctttgtc agcaaattct cccctcggct ataagactct
      241 gcctgcaggg ttaaccaatt gggggatctg taataaagct gttgagcaat gtagcgaggc
      301 aaatccctat ctaacgggaa agtaagatgc gataaagctt gcttggcaaa aggcagacgc
      361 aagatgtcca ataaatttcc ctcgccaaat ggaaaaacct ttgtcagaca gtggacctta
      421 cctcgtaagt cttcgtgaat aaggtaacga agacgcaatt cttttttctc gtcatccaat
      481 tcccagaggt gaaaacccat gttcatacta aaagagagaa attgcttgtg taatttactt
      541 aatctttctt ttatccatag gccttttccc aacagccaga gaacctgaag accagcttca
      601 tgataggctt gcgtcctcag ctgcaaacga gaaatgggca gactggaaca ttggacttcc
      661 aaagctagac tattattaac aaataaatca gcgacctgcc ctatactagg caggcatttt
      721 tcaagttcaa cttgttcggc attgattaac caactgtaca gacaagactt aagtgacaga
      781 tgctgggctg actcattctc ggaaaaatag tggcagtccc gcagggttac atgggcaaaa
      841 tgcgaacgca gaacttttcc ggatttgtat cggaccagcc ctccacaacc tggacaagaa
      901 tagcgacctt gcggcgcagg attttccaag acattaaaaa cttgtccatc ttcatcaagt
      961 gcaactaaca taaaacctcc tctctttatt agttcgcaaa aaaacaccca gctatgctga
     1021 gtgtttgaat tttcttaccc aatctgactg ccatttggca cagatggatc gactgttaag
     1081 agtattaaat tgtcaccatg ttcggctgag aggatcatgc cctgactgag caagcccatc
     1141 atcttacgtg gcttgaggtt ggcaacgatt tggactttct tgccgaccaa ctcctgctcg
     1201 ttcggataga acttggcgat accagacagg atttggcggt cttcgccatc acctgcgtcc
     1261 aggcggaatt tgagcagctt gtcagaaccc tccactttgg ctacttcctt gacttcggcc
     1321 acacggattt ctaccttgtc gaagtcctcg aacttgatcg cagctttttc gtttttgagt
     1381 tccacgtcag ctgggttcca ctctttttct tcctcttggg aaatagcaga gctgccaccc
     1441 atttgagctt ggatataggc gatttcctct tccatatcca gacgtgggaa gataggcgtt
     1501 ccttttgcga ccactgtcaa acctgctgga agacctgcaa agtcaagatt ttcaaggtca
     1561 aatgctgttc ccaaaccaag ttgttccata atagcattag acgtcgtcat cataaatggt
     1621 tgaatcaaat gtgccaccac acgaagacct gctgtcaagt gagccatcac cgctgccaac
     1681 ttgtcacggt ccgcttcgtc cttagccaag acccaaggtg cagtttcatc aatgtacttg
     1741 ttggtacggg agatgatatt ccaaacagct tccagagcgc gtggatagtc aacagcttcc
     1801 atatacttgt ggtagcttgc caagttgtca gctactactg ccgccaagtc cgcatcaaaa
     1861 tcagtcacgt tagcaacaaa ggttggtacc tgaccgctga aatacttatt aatcatagca
     1921 accgttcggt tgagcaagtt tccaaggtca ttggctagtt cgtagttgat acggccaacg
     1981 tagtcttctg gcgtgaaggt tccgtcagag ccaactggca gactacgcat gaggtaatag
     2041 cggagcggat ccaaaccaaa acgctcgacc agcatttctg gatagaccac attgccctta
     2101 gacttggaca tcttgccatc tttcatgaca aaccagccat gagcaaccaa gaggtctggc
     2161 aacttcatat ccaacatcat gagcatgatt ggccagtaaa tggagtggaa acgaagaatg
     2221 tccttgccaa ccatgtggta aactgttcca ttccagaact tgtcgtaatt aattgtttct
     2281 tcctgaccat aacccaaggc agttgcgtag ttgagcaggg catcaatcca gacatagaca
     2341 acgtgtttag gattggacgg aacaggcacg ccccaagtaa atgaggtacg ggacactgcc
     2401 aaatcttcca agcctggctc gatgaagttt ttcatgattt cattcatacg accatctggc
     2461 tggataaagt ctgggtgagc attgaagaac tcgaccaact tatcctgata cttgctgagg
     2521 cgaaggaagt aggattcttc tgaaacccag gcaacctcgt ggccgctcgg agcgataccg
     2581 cctgttacct tgccgttttc atctcggaag acctcttcta attggctctc ggtgaagaat
     2641 tcctcgtccg atacggagta ccaaccagaa tactctccca agtaaatgtc gtcctgcgcc
     2701 agcaattttt caaaaacagc cgccaccact tcctcgtggt aatcgtcggt tgtgcggatg
     2761 aacttgtcgt acgagatgtc gagcaagttc cagagctctt taacgccaac cgccatgccg
     2821 tcaacgtagg cttgaggtgt caaaccagct tcttttgcct tctcctcaat cttctgccca
     2881 tgctcatcaa gccctgtcag atagaagacc tcgtggccca tgagacgctt gtagcgagcc
     2941 aagacatcac aggcaatggt cgtgtaggct gaaccgatgt gaagttttcc agacggataa
     3001 taaatcggtg tggtgatata aaacggtgtt tttgtcatac tttttccttt caactcaggg
     3061 caaatgaaac cctgttcttg ataacacccc attttatcat attttcagct aaatcacaaa
     3121 atggttctaa ctacaattct tcctctatct atgctatact atgatacaac tcaaacagaa
     3181 agaggtcatc tatgtttaca aagaaaaata ttcttactca tctctttttc ctcatcatag
     3241 cagtcggact aaaacggtac ggtgtcacag cacctgactt gacatggaat atgttcttgg
     3301 cattggtagc acttgatttt gcaatcctta ccaatcatac aaagtcccat atcctcaaag
     3361 tgatagcagg tctcctctgg ctctttttct accccaatac tttttacatg gtgacagaca
     3421 tcgtccatat gcactttgcc aacacagtac tatggcaacg agaaagcatg attctattta
     3481 tgctttatgt tccaagtatt ttctttgggg tcatggctgg cgttgaaagt gtacgactta
     3541 ttttctctgc tttttctatc aaatcctact ggctaaaact gtttatgatt agtggtcttt
     3601 ccctactatc cagctttgcc attcatatcg gacgatatgc aagactgaat tcatgggaca
     3661 tctttaccag accaactgtc gttattgatg aaatgcttgc agttatttcg tgggatgctc
     3721 tcccctttat cctcggcttc acctttatcc aaattatggt tcttgtcttt tcaataaatg
     3781 aataagattt ttatctcata gccattatct tcaagcatct tcctgcactc aaggactatc
     3841 gttccttttg ggaagatttt tcaaggacat aaacaaaatt ttttgataaa tttatatctt
     3901 ctccgtttct ctccattaca tttttatcca aactgtatga ggcaaaaatc gttaacctat
     3961 tgtaagtaaa atttcttaaa tttccttata tataaaatat tcagaattta ctgtaaattc
     4021 tacataaaat catggatatt gattttaaat tcttaaaaat aatggtataa ttgaggaacc
     4081 tataaatgaa agagggagat ctattgttta caccaatcag cgaaaaaatt gacatcaatc
     4141 aagtacgaga acattcaaaa ctttctccag aaacacttgc taaaaaacaa gctcgtgacc
     4201 gtgaacttga agccatctta aaaggtgaag ataatcgtct tctcctagta attggaccat
     4261 gttcttctga caatgaagag gctgtcttag attatgctca ccgcttggcc aagctccaag
     4321 aagaggtcaa agataaaatt tttatggtca tgcgcgtcta tactgccaag ccacgtacca
     4381 atggtgatgg ttataaagga ctcatgcacc aaccagatac agatgctgca ccaagcctca
     4441 tcaacggaat caaagcagtt cgtaacctcc actatcgtgt cattacggaa acaggtttaa
     4501 ctactgctga cgagatgctc taccctgaaa acctgccttt ggttgatgac ttggtttcct
     4561 acatcgctat cggagctcgt tcggttgaaa atcagcagca ccgttttgta gcgtcaggca
     4621 tcgatgttcc aacaggcttg aaaaatccaa catctggtaa cctcaaagtc atgttcaacg
     4681 gtctttttgc tgctcagaag aagcaatcct tcctcttcaa caatactgaa gttgaaacat
     4741 ctggcaaccc acttactcac gttattcttc gtggcgcagt caatgaaaat ggtaaatacc
     4801 taccaaacta ttactatgac aatcttctag aaaccattga cctctatgat caatttggtt
     4861 tgaaaaatcc atttattatt atcgatacca accatgacaa ttctgggaaa aattatttgg
     4921 aacaaatccg tatcgtccgt caaaccttaa tcaaccgtga ctggaacgaa tccattcgta
     4981 actacgttcg cggattcatg attgaatcct acattgaaga cggtcgccaa gacaatccag
     5041 atgtctatgg aaaatccatc acagaccctt gcttaggctg ggaaaaaaca caagaactca
     5101 tccgagaaat ttacaacaga taggaagata ttatgggaat tcacaaacgt agcaccagtt
     5161 tagacatcga taaagtaaag gaactttcca agttagaagg agaatttcta gctgcaaaaa
     5221 atcggcgcga tgaagaactg aaaagaatta tccgaggcga agatgatcgt ctgctcttgg
     5281 ttatcggtcc ttgctcatca gataatgaag aggctgttct agaatatgca cgtcgtctct
     5341 ctaagcttca agaggaagtc aaagataaaa tcttcatggt catgcgggtt tacacagcta
     5401 agccacgtac caatggggaa ggctataaag gactggttca tcagccagat acttctaaat
     5461 taccagacct cattaacgga attgcagccg ttcgtaattt gcattaccgt gtcattactg
     5521 agaccggact cacaactgct gatgaaatgc tctactctgc caactatcca ctggtggaag
     5581 acttggtgtc ctaccatgct atcggagcac gttcggttga agaccaagaa catagatttg
     5641 ttgcatcagg tgtcgatatg ccaactggta tgaaaaaccc aacttctggt aatttgaccg
     5701 ttatgttcaa tggtatctac gccgcacaaa acaaacagaa ctttatttac cgtgatgcag
     5761 aagttgatac agatggcaac cctctagctc atgctatcct tcgtggtgcc aatactgaaa
     5821 agggcggtta tgagccaaac tactactatg atattctctt gaaaaccatc aaacagtatg
     5881 aacaatttgg tcttaaaaat ccatttatcg taattgatac caaccacgac aattctggta
     5941 aaacttacct ggaacaaatc cgtatcgtcc gtcaaaccct catcaaccga gcttggaacg
     6001 aatctattcg caaatatgtc cgtggtttca tgattgaatc ctacttagaa gacggccgtc
     6061 aagatagccc tgaggtattc ggcaaatcca ttaccgaccc ttgcttaggt tggaaaaaaa
     6121 cggaagctct gattcgtgaa attcatcaaa ccgtctaatt ctttccttaa ttttttatac
     6181 gataaataat ttggaggtaa gctatgaaca ttgatggtta cactcgtctt gctgccgttg
     6241 ttgcaaagcc aatcaaacac tccatctccc ccttcattca taatctggct tttaaggaaa
     6301 caggtgtaaa tggtgtctat gtcgcttggg aaattcccga ggaagatttg gctgtaacgc
     6361 ttgaaaatat caaacgatat gatatgtttg gtatcaacct ttccatgccc tataaacaag
     6421 ctgtcattcc ttatctagat gatttaacag cctctgcacg cctgattggc gcagtcaata
     6481 ccgtgattca taaagatgga aaactaatcg gtcacaacac cgatggaatt ggatttttca
     6541 agagtttgga aagactaaaa ggctttcaag ttaagaacaa gcgactaacc atcctaggtg
     6601 gtggtggtgc ttctactgct atcatagctc aggctacatt ggatggtgca aaggaaatta
     6661 ccattttctg ccgtcaacaa agcttggaaa gaacacaggc gagtcttaca cctattgctc
     6721 aagcaactgg agttcccatg caggtcctag cgcttgatga tagccaatta ctacaggaac
     6781 atatcaccaa ctccgactta ctagtcaacg gaaccagtgt aggcatggat gggtattctc
     6841 aacctgttcc ttctaccatt cgctttcccg aaaatctttt ggttgcggat gtgatttatc
     6901 aaccattcga aacaccttta ttaaaactag cccaatcaca aggcaaccct actattaacg
     6961 gtttgggcat gctccttttc caagcggctg aagcattcca agcctggact ggtaaggaaa
     7021 tgccgactga cttgatttgg gaccaattag tccagaaata cgacatcaaa tagagaggaa
     7081 atctgatgaa actgaccgtc aatcttccca acactcccta cgacatcctt atccaaagag
     7141 gaagtttagc acagacaggt acatggatca aagaactttg gaaacctcag aaaatcgcca
     7201 ttattacaga tgaccacgtt ggctcactct atcgcgaaac agttcagtca agcttagaac
     7261 aagctggttt tgaaaccatt gtttttgaat ttccagaagg tgaagcctca aaaaatcttg
     7321 ataccgtcaa ccaggcctac gaatttctcg taaaaaatgg tatgacacga agtgatggaa
     7381 tcattgctct cggcggggga gtcgttggcg atttggcagg ttttgtcgct tctacctata
     7441 tgcgaggtat ccatttccta caaatcccaa ccagcctaac tgcccaagtc gattcttcta
     7501 tcgggggaaa aactggcgtg aacaccccat ttgccaaaaa tatggtgggg accttctgcc
     7561 agccagacgg cgttctcatt gaccctgata ccctaaaaac actcggcaaa cgagaattga
     7621 ttgaaggaat gggcgaggtt gtcaaatatg gcttaataga tgatgtggag ctctgggaaa
     7681 ccctagacca attagacggc tctgtcgaga gcattctgga gcatgctgac tatatcatct
     7741 accactcctg tgaagtcaaa cgcaaggttg ttgtcgaaga tgaattagac aacggtgtca
     7801 gactctacct caacttcggt cacacgattg gacatgctat tgaagtgacc gctggctacg
     7861 gccaggtcat gcacggtgaa gctgttgcca ttggtatggt acaaatctca cgcgcagctg
     7921 agaaaaaagg actgatgcca gctggtatga cagaaaagat tatcgccatg tgtgaaaaat
     7981 tcggcttgcc gactacacac cagccttgga atgtagatga attatatgcc accctaactc
     8041 acgataaaaa agcacgtggc aagaccatca aacttgtcat cgttcctcaa ttgggacagg
     8101 ctgccatcca tcaaattcca atggaagaaa tgctagaatt tctacaagta tagtcattta
     8161 gttgactttt acgtcgaagt aataaaagtc aactaatact caatgaaaat caaaaacaga
     8221 ctaggcgacg cagatgcaga tagaactgaa gttcatcaaa tcaagtcaac aacgtctgat
     8281 tttgattttc gaagagtata aatgacgata taagaaaaaa gactactgac ttgcatgaaa
     8341 tcagtagtct tattttttat aaaatcatct catcgtcagt tagcttttca ccgctgtttt
     8401 tgtagaaaag gtccatcaag ttttgaacag tcaagttctt cttctcttct ccttctacat
     8461 cgacaacaat ctgaccacgg tgcaacatga ccaaacgatt accatattca atggcgtttt
     8521 ccatattatg cgtaatcatc agagctgtca actggtggct ctcgacaatc ttcttagtca
     8581 attccataac catatcgctg ttttttggat caagggcagc agtgtgttcg tccaggagca
     8641 gaacttttgg tttaaccagc gaagccatca tcaaggtcag agcctgacgt tgaccacctg
     8701 aaagaaattg cgtgtccacc ttcatacggt tttctagtcc caggccaagc tctttcaagg
     8761 cttccttaag aatagcacgt tcactatcct tcacacccca gccaagacca cgtgacaatc
     8821 cacgacgata ggcaatagcc atgttttctt caatggtcaa tcgagaagct gttcccatct
     8881 ttggatcttg aaatactcga ctaatgtcct tagagcgttt ggcagctgat acatgcttaa
     8941 tggatttacc ttcaagcaaa atatcgcccg aatcaaccgt caaggcacct gcaagtgagt
     9001 tcatgaaagt tgatttacca gcaccattac caccgatgat agagataaaa tcaccttctt
     9061 ttacatctaa gttcaaccca cgaaggacat ggttctcatt aactgttcca gcttcgaagg
     9121 ttttatgaat attttggatt gataaaattg tagacataac ttcctcctaa gcatttccat
     9181 tcaattttgg acgacgaata ttcaatttct tctgcaattc tggcacatag agaacggttg
     9241 ccagaaggat agctgagaag agtttaacaa ggtctgcatc catacttgga atttccaaaa
     9301 tcgctaagat aatcaaacgg taaacaactg caccgagaac aaccgatagg agtctccaac
     9361 ccaagcgtaa gttacgcaag ataacttctg caataatgac agatgctaga ccgataacaa
     9421 tggtaccagt acctgagttg agatctgcat agccattatt ctgagtaaga agagcaccac
     9481 aaagggcaat tagaccattc gacaacatat agccgtagat tttcatattg tctacattga
     9541 taccattggc ctcactcatt ggaatattat cccctgttga acgaatagct agaccaattt
     9601 gtgtattgag caagagcgtc aataagccta caactgctag tacaaagacc agaccaatca
     9661 ccaaaacagc atttgttttt gtcaaaccga aatcctgcaa ttgcgtcacc agtgtttctt
     9721 gtttgagcag ggctacgttg gctttgccta agattttcaa gttgatagag tagagacctg
     9781 tcaaggttac aataccggtc aaaagggctg gaatttttag ttttgtatga agtaaaccag
     9841 aaatcaagcc tgcacccata ccagctacca tagccataaa ggttgccagc aagggattca
     9901 ctccgttcac aatccctgtc gcacaaacag cagctccaag cggataagat ccctcagctg
     9961 tcatatcagc aatatctaaa atacgaaacg tcaagtaaac accaatcgcc ataactgacc
    10021 aaagcaagcc ttgcgagata cttgataaaa taatatccac gattttctcc tttttctaaa
    10081 ttcgtagtca atactaggtt ctatcttacc gatcttattc agatacagca agcttagaag
    10141 tatcgatacc taatttctga gccatttctt cattaacgtg aagactgacg gtctctgggt
    10201 attctactgc tgtttctgac acattggctc cttcgataat cttaatagcc atttttgcag
    10261 tttgacgacc taaagcttca tagttggtac cgtaagtcaa aagacctcct tcatccacca
    10321 tgtccgttga accaccgatt actggaacct tatgctccac agataattca ccgatcatgg
    10381 tcatggttga agcgacagta ttgtcggttg gaataaagag agcatctaca tccttcatca
    10441 agctagttgt cgcatcttgg acttcatttg aagatgtgat tccttttact actaccttat
    10501 aacctgcttt ttctagcaat tccttagctt gctctacctg aacttctgaa ttacgttcgc
    10561 tagtagtgta caaaatacca acggtcttag catctggaac agcttgacct agcaattcaa
    10621 cttgtttatc aattggagct tggtcagatg tacctgtcaa aagtccacct ggtttttcaa
    10681 tggattctac caagccagct gacaagggat ctgtaaccgc agtaaagaca atcggcgttt
    10741 cattagacac agtcgctaaa ctctgggcag acggcgtagc gattgccaag acaatatcat
    10801 ttccatcgat taattgttct gaaatggttt gaaggttagc ttgatcgcct tgggcatttt
    10861 gataatccaa aaccaatttt tcaccctctt tataaccatt tgcttctaat tccgccacaa
    10921 aaccctcacg ggcagctgtc aatgattcat gctccatata ttggagaact ccaactttga
    10981 ccacatctga ggaagcttgt tctgatgatg aggaacaagc tgcaagtgtt agcaaaccca
    11041 tacttgctac tgaaatagtt gccaattttt ttactagctt ataatacata aacgtttctt
    11101 ctcctaaata tattactctt ctaatgcttc tatagtcgct gcatcaatac caacagcttt
    11161 agccatttct tcattgacgg taatagctgc tgtatttggt ttttcaactg ctaagtcagc
    11221 aggcttttca cccttcaaaa tcttcacagc caattcacca gcttgaacac cgatagcatg
    11281 gtagtcgaca ccgtaggtaa atagagctgc gtcaataact gccgcgtcac ttcccatagc
    11341 tggaactttt gcttctttta aaatatcacc gattgttgaa gctgtagaag caactgtgtt
    11401 atcagttgga agatagatag catcgacttt tcctgcaagt gaagtaatag cttgctgaac
    11461 atcatttgtc gtcgtaactg ttgtcacttc tacttttaca ccttttgctt cgagtgcttt
    11521 tttagcctgc tcagcttgaa cttctgagtt tacctcgcta gaattgtaga aaataccgac
    11581 agtcttcgca gttggtacaa ctttgagcaa catctcgatt tgaccttcca catcagttgc
    11641 atcagtcgat cccgtcatgg tcccacctgg tttttccaat gattcaacca aacctgcttc
    11701 aactggatct gtgacagctg tgaatacact tgatgtttca ctatctgcat taagcatggc
    11761 ttgggctgcc ggtgttgcga tggcaaagtt aagatcattt tttcctgcca atttctcaac
    11821 cattgtctgt aagtttgctt gatcaccttg ggcattttgc aaatcaatcg tcagattttc
    11881 accttccttg tagcctgctt cttcgagcac ctctacaaag ccttcacgag ctgagctcaa
    11941 cgcttcatgt tcagcatatt gaataatacc aacattgact gaacttgaat ctgacgaatt
    12001 ggaactgcat gcagcaagcg ttactgctga caaaagtgct actgaactaa gtataatttt
    12061 tttaaatccc atgattattc tccttttata aatataatag caaacaagaa aggaccgctt
    12121 agactacact ctaaacggtc cttatatttc atctgtatag gaaaacagaa tgaattatcc
    12181 gtgccattta gatgtatcat ctatgaggca catgtcaaaa tagctacagc cacatagata
    12241 caaactttac gttcccgtgt ttgcatccat gtttaccatg tctacaaaca ctatctaaaa
    12301 tagccgtaga aataagtatg attagctgaa tgattttgac acatttgtaa catctctgtt
    12361 tccctttctc atttatttgt aactatcata actgttttca aattatttgt caataggaaa
    12421 ttgcgtaaat tttcagattt ttcaagtttt ttcgaatttt tctaaaaata gccccctata
    12481 ttattagcta taaaacgacc tgtcatcatg tttatgtagc cttttagttt acttttagta
    12541 cttgctccaa ctatttcaca aatagtcaga ggttggaaat aaggggaaca aggtttgcgt
    12601 caacaaccgc agctataact caatttctag cattgacttg aatcagtccc ctagattgct
    12661 tgcacagtaa atggactgtt caaggtgaga gatataagag cggattgcaa aatcaggtca
    12721 acaacgactg attttgattt tcgaagagta taaatgacta tatttgatgt aacaaaaaag
    12781 tctgaaccta agcccagacc ctactaaaac aattatttta cttcaacttt ttcagtccaa
    12841 ggacacgctg tagttgctag aacctcgttt agttcttcta cgttgcggcg gccttgatcc
    12901 gccaaccaag ctttggctgc ttcttcaccc tcgcttgcaa agatggctac tgcatccttc
    12961 caagtcgccc gaccacaaag aacaccatta aagcttgaac cagcttcttt ggcaaagaca
    13021 agggtttctt ggaaaagttt agcagatacg ccagcgctca aataaatgaa tggcaagtga
    13081 gtgctatctg tttgttcctt gaagaaggcc ttagcttcct caactgtata gactggctct
    13141 tcatctgtgt aaccttctac aaagttcata ttgacaggta cttcaacctt gagcacgtcg
    13201 gcattataac gtgacttgct aaattcacgc atggcaccat tgaccttgtg tggtttgagg
    13261 gctgcgtatt cacgagaagt cacatccata tcgctggcat cataagtcaa gatttcgacg
    13321 aagtaaggaa tatcttctgc gatacactca ctaccaatgc gttctaccca agcatgctta
    13381 atgtcgttga tttctggctt gtcatctaca tcatagtaaa gcaagatttt tacggcatcc
    13441 gcccccaatt ctttgatacg tttggctgac caatttggca aaaggtctgg caaacgacct
    13501 ggtgtggacg catcataacc tgttttttca taagctgcaa tgaaaccgca atctgcatgg
    13561 cgcaattctg aagctggaac accgtattcc gcatccaaga gaatggagct tgcatacgga
    13621 gtcaaatcgc ttgagatgac tttcttgaag tcaatcaaaa tgtcatctcc aaattcgcca
    13681 ccacctgctg cagcagcaag caggcgtttc aatgcaccac gttggtcaat cgccaaggcc
    13741 gcaatcacct gatcgctgtt tgacaagcgg gtcatgtgat tgaattttgc ctgagaaagt
    13801 tgtaattttg tcattagttt cctcctaagg aattttatta accaaatcgt ttcacgccat
    13861 ctagataggt cgcttgtaag cgtcctgcat catctacgac gataaagtct gcagctcggc
    13921 cttctgcaat ccgaccacaa atatggtcaa tattgacaga gcgagctggt gccagagagg
    13981 ccatacgaag ggcatcaggt agggatacca agccccattt cacaacattt tgcactgctt
    14041 cgatcaattc taagatagaa cctgccaaac taccactttc ttttaaacga gcagttccat
    14101 cttttacaac aacttcaaat tcaccgaggc gggactcccc ttcacccata ccgccagctc
    14161 gcatacagtc cgtaatcaaa acagtttcct ctgctccacg tgctttgacc acgatttctg
    14221 ccgcaactgg atgaacatgg tgtccatcac aaatcatttc cgcatagaca ttttttaaat
    14281 tcaaagcagc accgaccata cctggttcac ggtgatgaag cccactcatc ccattgtaaa
    14341 catggacgaa gatattggca ccagcttcta ctgccgcttc cgcttgcgta taggtcgcat
    14401 cgctgtgagc caaggcagta tgaattgcct tgctgttagc aaactcgatg aattctttga
    14461 cgccttcacg ttctggagca atagcaattt tattgactaa acctttagat aagctgttcc
    14521 agttgtccaa cttttcaatg gacggatcgc tcatgtactt aggattttgt gctcccttgt
    14581 atttttctgt aaagaaggga ccttctaaga aaataccttg aatcttggcg cctgtttcct
    14641 gccctgcata agtcccaatc gtttcacaaa ctgcatctaa attttcagtc gtatctgtta
    14701 aggtcgttgg aagccaagag gtcaccccac aagacaagag accttctgaa attaccttga
    14761 tcccttcaaa atcattgtcc ataacatcgt gagaagcgta gccgtggata tgtgtatcaa
    14821 ctagccctgg tgctaaatga aagtcagaat aatcaacaat ctccccttca ggcttttcag
    14881 tcacaatccg tccaaactga cccttgtcag taatttccaa ataggctgat tcgacctcac
    14941 tctctggtaa aataatagat tttgctttta taaatactg
//
LOCUS       DAZQCJ010000043        14370 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000043 DAZQCJ010000000
VERSION     DAZQCJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14370
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.044"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            87..452
                     /gene="mscL"
                     /locus_tag="ACHU66_001197"
     CDS             87..452
                     /gene="mscL"
                     /locus_tag="ACHU66_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFR3389485.1"
                     /translation="MLKDLKAFLFRGNIVDLAVAVVIGGAFGAIIKSFVNDIITPLIL
                     NPALKAANVQNIADLAWNGVKYGSFLSAVINFLIIGTSMFFVVKAAEKAMPKKVEEKA
                     PAGPTQEELLAEIRDLLKK"
     gene            complement(685..1698)
                     /gene="galE"
                     /locus_tag="ACHU66_001198"
     CDS             complement(685..1698)
                     /gene="galE"
                     /locus_tag="ACHU66_001198"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR3389486.1"
                     /translation="MSILVTGGAGYIGSHTVVELLKLGKEVVIVDNLSNSSILVLDRI
                     ETITGKRPTFYELDVADKEALRQVFENENIEAAIHFAGYKAVGESVAKPIMYYENNIM
                     STLALVEVMTEFSVKKIVFSSSATVYGLNNPSPLVETMPTSATNPYGYTKVMLEQILR
                     DVEVADKEWSIALLRYFNPIGAHESGLIGEDPAGIPNNLMPFISQVAVGKREELSVFG
                     NDYDTIDGTGVRDYIHVIDLALGHIKALEKISTTTGVHTYNLGSGQGTSVLELVKAFE
                     KVNGVPVHYKLVDRRPGDVATCYANADKAWKELNWKTEKTIEDMCRDTWNWQSKNPNG
                     YEG"
     gene            complement(1802..1978)
                     /gene="rpsU"
                     /locus_tag="ACHU66_001199"
     CDS             complement(1802..1978)
                     /gene="rpsU"
                     /locus_tag="ACHU66_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR3389487.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEAARKRKKF"
     gene            complement(2076..2705)
                     /locus_tag="ACHU66_001200"
     CDS             complement(2076..2705)
                     /locus_tag="ACHU66_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFR3389488.1"
                     /translation="MRTKLQIIGTILFVLSAAVLGTIYLAWLVYPLEISFLGLEKVVY
                     MKVADISYNFNILMNYLTNPFASVLDMPNFSSSADGLKHFADVKHLFHLTQGIFILTL
                     PAFVLFVKNILLKGYGDLVKKVIFWTMLTPMIIGLLGVLVGFDQFFALFHTVLFPGDS
                     TWLFDPAKDPVIYILPQEFFLHCFVLFFVLYEFFFWTILAWTGKKNRIG"
     gene            complement(2695..3468)
                     /locus_tag="ACHU66_001201"
     CDS             complement(2695..3468)
                     /locus_tag="ACHU66_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFR3389489.1"
                     /translation="MTYTGYLIDLDGTIYEGKKRIPAGERFIHRLQECQIPYLFVTNN
                     TTRRPEMVQAMLAENFNIETPLETIYTASLATVDYMNDLGKEKTVYVIGEDGLKSAIF
                     EAGYVEDTENPAYVVVGLDTQLTYEKLTIATLAIQKGATFIGTNPDLNIPTERGHLPG
                     AGSLIALLKAATRVEPTFIGKPEVIIMDKALEILGTERSQTVMVGDNYLTDIRAGIDN
                     GFPTLLVLTGFTKPEEVADLPLAPTHVLNSLDEWSFDEN"
     gene            complement(3478..4095)
                     /locus_tag="ACHU66_001202"
     CDS             complement(3478..4095)
                     /locus_tag="ACHU66_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HFR3389490.1"
                     /translation="MSQDKWLEWAVRLQALAQTGLAYGKDVYDMERFEEIRQIAAEML
                     VEPSGQPLEVVKDLFCNETGYQTPKLDTRAAIFQEDKILLVQENDGLWSLPGGWCDVD
                     QSVKDNVVKEVKEEAGLDVEAQRVVAILDKHKNNPAKSAHRVTKVFILCRLLGGEFQP
                     NSETVASGFFSLDDLPPLYLGKNTAEQLALCLEASRSEHWETRFD"
     gene            complement(4082..4822)
                     /locus_tag="ACHU66_001203"
     CDS             complement(4082..4822)
                     /locus_tag="ACHU66_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFR3389491.1"
                     /translation="MGLTYQEEFTIPFDMVDVKQEIKLPDFISYCLGVSGRQSEELGR
                     SDLYVFQEFGLIWVVTDYELTIQRLPKYNETITIKTEAVAYNKFFCHRMFYIYDAAGN
                     LLLDILCYFVLIDFETRKVAPVPEDLIAPYQSEQVKKLPRAPKYQLLENPSVQEFPVR
                     YFDLDMNGHVNNGKYLEWMYEALGYDFLLCHVPKKIQLKYLKEVEATSLVSSRMVAEA
                     GGSQHEIVVDGHIHAQAVIEWRERHVAG"
     gene            complement(5053..6180)
                     /gene="hemW"
                     /locus_tag="ACHU66_001204"
     CDS             complement(5053..6180)
                     /gene="hemW"
                     /locus_tag="ACHU66_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR3389492.1"
                     /translation="MKKRPTSAYIHIPFCTQICYYCDFSKVFIKNQPVDEYLAALMEE
                     VKFYDLPALRTLYIGGGTPSALSADQLDYLLTNLEDLLDLSQLEEFTIEANPGDLTAD
                     KIAVLKKSKCNRVSLGVQTFDDRMLKKIGRSHNQAQIYETIATLKEAGFYNISIDLIY
                     ALPGQTMEQVVDNVAKALELDIPHMSLYSLILENHTVFMNRERRGNLHLPNEDVESDM
                     FDYILQELEKNGFEHYEISNFTKPGFESRHNLMYWDNSEYYGLGAGASGYIDGMRYRN
                     RGPIQHYLKSIREKGHSRLHEEFLSQTEQMEEEMFLGLRKKTGVSIERFEEKFGISFE
                     DRYGQVVRDLKNEGLLQEEDRWLRMTKKGLFLGDTVAERFI"
     gene            6275..6853
                     /locus_tag="ACHU66_001205"
     CDS             6275..6853
                     /locus_tag="ACHU66_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFR3389493.1"
                     /translation="MKKEIIYDCINLILSFLLCRWLFMTFLFYQFSNIFMTIDFWPTL
                     AAILLMTGFCFTLFRLVYKPHISRLTLWFFYACYGLLLVYLLFFKSMGVRGVNWNLLS
                     TFSQDLFLNPTILVFNLLLFLPLGLLFSFSWKKLSLFVGAILLVEACQFFFSLGFFDL
                     GDILLNTSGFALGNFLGQSAIAQSFKNRIQKK"
     gene            complement(6988..9255)
                     /locus_tag="ACHU66_001206"
     CDS             complement(6988..9255)
                     /locus_tag="ACHU66_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HFR3389494.1"
                     /translation="MINLQNFVQSMYAKRIEDCTDQELYYALLAFTKQQSEAKYTNDQ
                     KKKVYYISAEFLIGKLLSNNLINLGLYDEVKSQLVAAGKDLIAIEDMELEPSLGNGGL
                     GRLAACFLDSIASLGLNGDGVGLNYHFGLFRQLFEYHQQSAVPNEWITPRSWLTESPI
                     SYQVPFANFTLTSKLYDIDVPGYKMERKNRLRLFDLGSVDDNIIYDGIEFDKEDIFRN
                     LTLFLYPDDSTDEGKVLRIFQQYFMVSNAAQLLIDEAVAKGSNLHDLPDYAVVQINDT
                     HPSLVIPELIRLLELRGISFDEAVEIVKKMTAYTNHTILSEALEKWPLDFLNYVVPHL
                     VPFIVELDRRAKEVKDDPAVNIIDEYGRVHMAHMDIHYGYSINGVAALHTEILKTSEL
                     KAFYELYPEKFNNKTNGITFRRWLMHANPSLASYLDGLLGHGYHHDASELEKLLDYKN
                     DKELKSWLEKTKQHNKRKLQRHIAKTQGVHVNPESIFDVQIKRMHEYKRQQMNVLYVI
                     HKYLDIKAGNIPTRPLTVFFGGKAAPAYTTAQDIIHLILVLSQVIKNDSEVAPHLQLV
                     MIENYNVTEASFIIPAADISEQISLASKEASGTGNMKFMLNGALTIGTDDGANVEIHE
                     LVGDENIYIFGQDSQTVIEHYANRTYHPYSFYEREVIRPLIDFITSDTIRHAGGIDER
                     LWRLQDNLKHNDHFMTLLDLEDYIVTKERMLADYEDRDSWLEKVIVNIAKAGFFSSDR
                     TIQQYNEDIWHLEAK"
     gene            complement(9432..10145)
                     /gene="deoD"
                     /locus_tag="ACHU66_001207"
     CDS             complement(9432..10145)
                     /gene="deoD"
                     /locus_tag="ACHU66_001207"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR3389495.1"
                     /translation="MSIHISAKPGEIADKILLPGDPLRAKFIAENFLEDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYAHELINEYGVKKLIRVGTAGSLNEDVHVRE
                     LVLAQAAATNSRMINIDWPEYDLPQIADFDLLDKAYHIAKELNMTTHVGNVLSSDSFY
                     SPKLFSRNLELGQLGVKAVEMEAAALYYLGAKFGVQTLAIMTISDSLVNPEENTTAEE
                     RQNTFTDMMKVGLETLIAE"
     gene            complement(10178..10987)
                     /locus_tag="ACHU66_001208"
     CDS             complement(10178..10987)
                     /locus_tag="ACHU66_001208"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR3389496.1"
                     /translation="MSLLAKINETKTFLEEKGLVKPEFGLILGSGLGELAQEVENAIV
                     IDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPMEVVTFPVRVMKA
                     LGCEGVIVTNAAGGIGYGPGTLMAITDHINMTGQNPLIGENLEEFGPRFPDMSNAYTK
                     EYREKAHAVAEKLGIKLDDGVYLGVTGPTYETPAEILAFKTMGAHAVGMSTVPEVIVA
                     AHSGMKVLGISAITNFAAGFQSELNHEEVVEVTEQIKGNFKGLVKAILAEL"
     gene            complement(10999..11355)
                     /locus_tag="ACHU66_001209"
     CDS             complement(10999..11355)
                     /locus_tag="ACHU66_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFR3389497.1"
                     /translation="MEKLTVYYNPNCSKCKKLRILLSSQDMEVKWVNYLETPLRGAEL
                     TALLEKMGSQPSAVTRLKEEERLALSEEEIFERLVEEPATLNRPIIEREQTAFLCRPL
                     EIIKEKMPEYDWSDYL"
     gene            complement(11355..12566)
                     /locus_tag="ACHU66_001210"
     CDS             complement(11355..12566)
                     /locus_tag="ACHU66_001210"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR3389498.1"
                     /translation="MPKFKRVHLVVMDSVGIGAAPDSDKFFNAGVADTESDTLGHISE
                     KAGLEVPNMTKIGLGNIPRDTALATVPAESHPTGYVTKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWDGFPEEILTKIEEFSGRKIIREANKPYSGTAVIDDFGPRQMETGELIV
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFSRT
                     ANRHDYAVSPFKATVLNKLADAGVPTYSVGKISDIFNGSGITNDMGHTKSNMHGVDVL
                     IDTLKLSDFEEGFSFTNLVDFDAVYGHRRNIEGYRDCLQEFDARIPEIIDNMREDDLL
                     LITADHGNDPSYAGTDHTREYVPLLAYSKAFKGSGVLPVGHFADISATVAENFGVDTA
                     MIGESFLSQLV"
     gene            complement(12895..13569)
                     /gene="rpiA"
                     /locus_tag="ACHU66_001211"
     CDS             complement(12895..13569)
                     /gene="rpiA"
                     /locus_tag="ACHU66_001211"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR3389499.1"
                     /translation="MTNLKEQVGIKAAEFVTDGMIVGLGTGSTAYYFVQEIGRRVAEE
                     GLQITGVTTSHATAEHAASLGIPLKNIDEVEYVDLTVDGADEVDGDFNGIKGGGAALL
                     MEKVVAVNSKDCIWIVDESKVVQTLGAFKLPVEVVQYGAENLFRLFEKKGYRPSFRMR
                     NGEKHITDMQNFIIDLDLRRIEDTYALAEELDRTVGVVEHGLFIGLISKVIVGTLEGP
                     NIIEKK"
     repeat_region   13671..14364
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=13737..13772
                     /rpt_unit_seq="gttttactgttacttaaatcttgagagtacaaaaac"
BASE COUNT         4125 a         3221 c         2766 g         4258 t
ORIGIN      
        1 aaatttctaa aacaaattgc ttgtaaaata ttttttatgg gtataataga taaagtaaaa
       61 tatttatgaa aggacatatt taggatatgt tgaaagattt aaaagcattt ttgttccgtg
      121 ggaacattgt tgaccttgct gtagcagtgg taattggtgg agcgttcggc gcaattatca
      181 aatcatttgt aaatgatatc atcactccat tgattttgaa cccagctttg aaagcggcaa
      241 atgtacaaaa tattgctgac cttgcatgga atggtgtgaa gtacggtagt ttcttgagcg
      301 ctgttattaa cttcctaatc attggcactt caatgttctt tgttgttaaa gcagctgaga
      361 aagcaatgcc taagaaagtt gaagagaaag ctcctgctgg tccaactcaa gaagagttgc
      421 ttgcagaaat ccgcgatttg ttgaaaaaat aatcaataat atattgggag tgggaaagaa
      481 ctcgaccagt ctaaaaagag ttcgtcttcc cacccccgca cagttgatta ggtcagattt
      541 ggagtatgaa acacgaacaa atctgccaat caaccactgc gctgagatgt tgacacgaac
      601 tctgagaagc gaggatggac tttctgccca gcctctcttc cataattaca aaaataagat
      661 gtgagcctcc actcacatct tttcttatcc ttcgtatcca tttggatttt tcgattgcca
      721 gttccatgtg tcgcggcaca tatcttcaat tgttttttct gttttccagt tcaactcttt
      781 ccaagccttg tctgcgttag cgtaacaggt tgccacatca cctggacgac ggtcaacaag
      841 cttataatgt actggaacgc catttacctt ttcaaaggct ttgaccaact ctaatacact
      901 tgttccttga cctgaaccaa gattgtaagt atgtacacct gtagtagttg aaattttttc
      961 aagcgccttg atatgaccga gtgccaaatc aatcacatgg atataatcac gcactccagt
     1021 accatcaatt gtatcataat cgttaccaaa aacgctgagc tcctcacgtt taccaactgc
     1081 tacctgtgaa ataaacggca tcaaattatt aggaatacca gctggatctt ccccaatcaa
     1141 gccagattca tgggcaccga ttggattgaa ataacggagc aatgcaatac tccactcctt
     1201 atctgctact tccacatcac gcaagatttg ttccaacata actttggtat agccgtacgg
     1261 attcgttgcg ctagttggca tagtttcaac caaaggcgat ggattattga ggccataaac
     1321 agtcgcactt gaggagaaga caattttctt aacactaaat tctgtcatga cttccaccaa
     1381 agctagggtt gacatgatat tgttttcata gtacataatt ggcttagcca ctgattctcc
     1441 tactgccttg tagccagcaa agtgaatggc tgcctcaatg ttttcatttt caaaaacttg
     1501 gcgcaaggct tccttatctg ccacgtccaa ctcatagaaa gttgggcgct tgccagtgat
     1561 ggtttcaatg cgatctaaaa ccaaaatact tgagtttgaa agattatcca cgataaccac
     1621 ttctttcccc aacttcaaca attcgacaac cgtatgacta ccaatataac ctgcaccacc
     1681 agtaactaag atactcatct ttttactcct tttcacctag acaaaattct atatccctat
     1741 tatatgttac ctgttgtaaa aagtaaacaa aaaacagcct ttcggccgtt ttcaaattca
     1801 attagaattt tttacgcttg cgagctgctt ctgatttacg tttacgtttt acagatggtt
     1861 tttcgtagaa ttcgcgtttg cgtgtttctt gaagagtacc agctttagta accgcacgtt
     1921 tgaaacgacg aagagcatca tcaagtgatt cgttcttacg tactactgtt tttgacattt
     1981 tttcactccc ctcaatttca aagtctttat cattttacta tgattcaact catttgtcaa
     2041 tatttttttc attctaaaat atgaatttta cgttcttatc ctatcctatt cttttttcct
     2101 gtccacgcca gtattgtcca aaagaagaat tcgtaaagga caaaaaataa tacaaagcaa
     2161 tgcaagaaaa actcttgggg caaaatatat atgacaggat cctttgcagg gtcaaatagc
     2221 catgtagaat ctcctggaaa tagcaccgta tgaaacaatg caaaaaactg atcaaagcca
     2281 acaagaactc ctaataaacc gattatcatt ggcgtaagca tggtccagaa aatgaccttt
     2341 ttaaccaagt ctccataacc ttttagcaga atgtttttta caaaaagcac aaaggcaggg
     2401 agggtgagga taaaaattcc ctgagttaga tggaataaat gtttcacatc cgcaaaatgt
     2461 ttcagaccat cagctgagga tgaaaaattt ggcatgtcta aaacacttgc aaaaggattt
     2521 gttagatagt tcatcaggat attgaaattg taggaaatat ctgccacctt catgtagacc
     2581 actttttcca accccaaaaa agaaatctca agcggataaa ctagccaagc taggtagatg
     2641 gttcctagaa ctgctgccga taagacaaat aaaatcgttc cgataatctg taacttagtt
     2701 ctcatcaaaa ctccattcat ctaggctgtt gaggacatgg gttggagcca ggggtaagtc
     2761 tgctacttct tctggcttgg taaatcctgt caggactaaa agagttggaa atccattgtc
     2821 aatccctgca cgaatatctg tcaggtagtt atctcctacc ataactgtct ggctgcgttc
     2881 tgtgcctaaa atctccagag ccttgtccat gataatgact tcaggcttac cgataaaggt
     2941 cggctctact cgggtagcag ctttcaaaag cgcaataagt gagcctgctc ctggcaaatg
     3001 acctctttca gtcggaatgt tcaaatcagg attggtaccg ataaaggtcg ctcccttttg
     3061 aatagctaag gtggcaatgg tcagtttttc ataggtcaac tgagtatcca aaccgacaac
     3121 aacataggct ggattttccg tgtcttccac ataacctgcc tcaaaaatgg cagacttgag
     3181 cccgtcttct ccaatgacat agactgtctt ttccttgccc aagtcattca tataatctac
     3241 tgtcgccaga ctggctgtat agattgtttc aagcggtgtc tcaatgttaa agttttcagc
     3301 caacatagcc tgcaccatct caggacggcg ggtggtattg ttggtcacaa ataaataggg
     3361 aatctgacat tcttgcaagc gatgaataaa acgttcacca gcaggaatcc gctttttccc
     3421 ttcataaatc gtaccatcca aatcaatcaa ataacctgta tatgtcattt ttcctcctta
     3481 atcaaatcgt gtttcccaat gctcagaccg actagcttcc aagcaaagtg ctaactgctc
     3541 agctgtattt ttcccaaggt agagcggtgg taagtcatct agactgaaaa agccactggc
     3601 aactgtttcc gaattgggct gaaactcccc gccaaggaga cgacatagaa tgaagacctt
     3661 ggtcacgcgg tgggctgact tggcaggatt gttcttgtgt ttgtccaaaa tcgccaccac
     3721 acgttgagcc tccacatcca gccccgcttc ttcctttact tccttgacta cattgtcctt
     3781 gacagactgg tccacatcgc accaaccacc tggtaaggac cagagcccat cgttttcctg
     3841 aaccagtagg attttatcct cttggaaaat agctgcgcgg gtatccagct tgggtgtctg
     3901 atagcctgtt tcattgcaaa acaagtcttt cactacttcc aagggctgtc cagatggctc
     3961 taccaacatt tccgccgcaa tctgtctgat ttcctcaaat cgctccatgt cataaacatc
     4021 ttttccatag gccaagcctg tctgagccaa ggcttgtaaa cgtacggccc attccaacca
     4081 cttatcctgc gacatgacgt tccctccatt ctatgacagc ttgcgcatgg atatgcccat
     4141 ctaccacaat ctcatgctga ctaccaccag cttctgccac catacgcgaa ctcaccaagc
     4201 tagttgcctc tacctcttta agatacttga gttgtatttt tttagggaca tggcagagca
     4261 gaaaatcata gcccagtgct tcatacatcc attccaaata cttgccatta ttgacatgcc
     4321 cattcatatc caagtcaaaa tagcgaacag gaaattcttg gacagatgga ttttccaaga
     4381 gctgatactt gggagcccga ggcaatttct tgacctgctc agactgataa ggagcaatca
     4441 aatcttctgg cacaggcgcc accttgcgag tttcaaaatc aatcaggaca aaatagcaga
     4501 gaatgtccaa caaaagattg cctgctgcat cgtagatata gaacatccga tgacagaaaa
     4561 acttgttgta ggcaaccgct tctgtcttaa tggtaatggt ttcattgtac ttaggtaaac
     4621 gttgaatggt cagttcataa tctgtcacca cccaaatcaa gccaaattcc tgaaaaacat
     4681 agaggtcact gcgacccagt tcttccgact gccgacctga tacaccaaga cagtaggaaa
     4741 taaagtcagg tagtttgatt tcttgtttga catccaccat atcaaaagga atggtgaact
     4801 cttcttggta tgttagtccc ataatgttga tctttctagt cttaaagtat agtctttcag
     4861 ttcacgtcaa cagctgcagg tagcttcaat agtccagtgg actattgaag gttggaaatc
     4921 gtacttacga tagtaagtaa cattcgtaga gtacggcaaa gtgagttcaa atactccagt
     4981 ggagtgtttg aagttggaaa taaggaaacg aagtttcttc gctcgtcgaa gtaataaaaa
     5041 caaactgaat gactatatga aacgctctgc aactgtatcg cccagaaaca accccttttt
     5101 agtcatccga agccaacggt cttcttcttg caaaagacct tcatttttca agtctctgac
     5161 cacctgaccg tagcggtctt caaaggaaat gccaaacttt tcctcgaatc gctcaatgga
     5221 cacgccagtt tttttccgta gccctaaaaa catctcttct tccatctgct cggtctggct
     5281 gaggaattct tcgtgtaagc gagaatggcc tttttcgcgg atggacttga ggtagtgctg
     5341 aatggggccg cggttgcgat agcgcatgcc gtcgatgtag ccagatgctc cagctcctag
     5401 accataatac tccgagttgt cccagtacat gagattgtgg cggctttcaa agccgggctt
     5461 ggtgaagttg gagatttcgt agtgctcaaa gccgtttttc tccaactcct gaagaatgta
     5521 gtcgaacata tcggactcca catcttcgtt gggtagatga agattgccac ggcgttcgcg
     5581 gttcatgaag accgtatggt tctccaaaat caagctatag aggctcatgt ggggaatgtc
     5641 cagctccaag gccttggcga cattatcgac cacctgctcc atggtctgac caggcagggc
     5701 ataaatcagg tcgatggaaa tattgtaaaa tcctgcttcc ttgagggtag caatggtttc
     5761 ataaatctgg gcctgattgt ggctgcgacc gatttttttc aacatgcggt cgtcaaaggt
     5821 ctgaacgccc agcgataccc gattgcactt ggattttttc agaactgcaa tcttgtctgc
     5881 cgtcaagtca cctggattgg cctctatagt gaattcttcc agctgtgaca agtccagcag
     5941 gtcctccaga ttggtcagga gataatctag ctggtctgcc gagagagctg acggtgttcc
     6001 gcctccaata taaagtgtgc ggagagctgg caggtcgtaa aacttgacct cttccataag
     6061 agctgccaaa tactcgtcaa caggctgatt cttaataaag accttggaaa aatcacagta
     6121 gtaacaaatc tgggtacaaa atggaatgtg gatataggca gatgtcggtc gttttttcat
     6181 accaactatt atagcacact gtctataaaa atattttcta cagccttgac ctcccctcaa
     6241 ataatgatat aatgaaataa aaaggagatc ctctatgaaa aaagagatta tctacgactg
     6301 tattaacctg atcctgtcat ttctgctttg ccgctggctc ttcatgacct ttctttttta
     6361 ccaattttca aacattttca tgacgattga tttttggccg acacttgccg caatcctgtt
     6421 aatgaccgga ttttgcttta ccctatttcg acttgtctat aaaccccaca tttcgcgact
     6481 gacactttgg tttttctacg cttgctacgg gcttctgcta gtctatcttc tctttttcaa
     6541 aagcatgggc gtcagaggtg tcaactggaa tctccttagc actttttctc aagacctatt
     6601 tctcaatcct actatattgg tttttaacct cttactcttt ttaccacttg ggttgctttt
     6661 ttcattttct tggaaaaaac tttctctctt tgtaggggcc atattgctag ttgaagcctg
     6721 tcaattcttc ttttcactgg gcttctttga ccttggtgac atcttgctta acacttctgg
     6781 ctttgccctt ggaaacttct tgggacaatc cgcaatcgct caatctttta aaaatagaat
     6841 acagaaaaaa tgaccccgca aggtcatttt tagtttattt atactcaatg aaaatcaaaa
     6901 gtagcctagg aaacgaagcc gacgatagaa ctgaagttca tctaggctct tgacaacgga
     6961 taattttgat tttcgaagag tattagctta cttagcttcc aaatgccaaa tatcttcatt
     7021 gtactgttga atcgtacggt ctgatgagaa gaaacctgct ttagcgatgt tgacgatgac
     7081 tttttccaac cagctgtcgc ggtcttcata gtcagccaac attctttcct tagtcacaat
     7141 atagtcttcc aaatctagca aggtcatgaa gtggtcattg tgcttgaggt tgtcctgcaa
     7201 acgccagaga cgctcatcga tcccaccagc atgacgaatg gtatctgatg taataaagtc
     7261 aatcaatgga cgaatgactt cacgttcgta gaaggaatat ggatggtagg tcctatttgc
     7321 atagtgttcg ataactgttt ggctatcttg accaaagata taaatatttt catcacctac
     7381 caactcatga atttccacat tggccccatc atctgtaccg atggtcaagg ctccattgag
     7441 catgaatttc atgttacctg taccagatgc ttccttagat gcaagtgaaa tttgctcaga
     7501 aatatccgct gctggaatga taaagctagc ttcggttacg ttgtagttct caatcataac
     7561 tagctgcaaa tgtggtgcca cttctgaatc atttttgata acctgtgata agaccagaat
     7621 caagtggatg atgtcttgag ctgttgtata ggctggagct gcttttcctc caaagaagac
     7681 tgttaacggg cgagtcggga tattgcccgc cttgatatcc aaatacttat ggataacata
     7741 gaggacattc atctgttgac gcttgtactc gtgcatgcgc ttgatttgta catcaaagat
     7801 ggattcagga ttgacatgta caccttgagt tttggcaata tgacgttgca acttccgctt
     7861 gttgtgttgc tttgtctttt ccaaccaaga tttcaattcc ttatcatttt tgtaatccaa
     7921 cagcttttct agttcacttg catcgtgatg gtagccatgt ccaagtaggc cgtccaggta
     7981 gcttgccaaa cttggattag catgcatgag ccagcgacgg aaggtaatcc catttgtctt
     8041 attattgaat ttttcaggat atagctcata gaaagctttc aactctgaag ttttcaaaat
     8101 ctctgtatga agtgccgcca ccccattgat agaataacca taatgaatat ccatgtgtgc
     8161 catgtgaaca cggccatatt catcgatgat attgacagca ggatcatcct tcacttcttt
     8221 ggcacgacga tccaattcta cgatgaacgg taccaagtgc ggaaccacat agttcagaaa
     8281 atcaagcggc catttttcca gcgcctctga caagatggtg tggttggtat aagcagtcat
     8341 tttcttaaca atttcaactg cctcatcaaa tgaaatacca cgaagctcca aaaggcgaat
     8401 caattctgga ataacaagtg atgggtgagt atcgttgatt tgaacaacag cataatcagg
     8461 caggtcatgt aagtttgacc ccttagcaac tgcttcgtca atcaatagtt gcgcagcgtt
     8521 agacaccatg aaatactgct ggaagatgcg gagaactttt ccttcatcgg tcgaatcatc
     8581 tggatagagg aagagggtca agtttcggaa aatatcttcc ttatcaaatt ctattccgtc
     8641 ataaatgata ttgtcatcaa ctgaaccaag gtcaaacaag cgaagacgat tcttacgctc
     8701 cattttataa ccaggtacat cgatgtcata caacttagat gtcaaggtaa agttagcaaa
     8761 tggcacctga taagaaatcg gtgactctgt taaccatgaa cgtggtgtaa tccactcgtt
     8821 tggcacagca gactgctgat ggtattcaaa taattgtcta aacagtccaa agtgataatt
     8881 caagccgacc ccatcaccgt taagacctag actagcaatt gaatcaagga agcaggcagc
     8941 caagcgcccc aaacctccat taccaagaga cggttcaagc tccatgtctt caatagcaat
     9001 caaatcctta ccagccgcca ccaactggct tttaacctca tcataaagac caaggttgat
     9061 taaattatta gacaagagct taccaattaa gaactctgct gaaatatagt agaccttttt
     9121 cttttggtca tttgtatact tagcttcact ctgctgtttt gtaaaagcaa gcaaggcata
     9181 atagagttct tggtctgtac aatcttcaat gcgtttggca tacatagact gaacaaaatt
     9241 ttgtaaatta atcatgagga ctctcctaga tatattaatg taatcattca gaaaggtttc
     9301 gctttgcctt tgtaaacgtt ttcttaatta taccctattt ataaaaaaaa agacaagcca
     9361 acttccgtac aaatagaaat tagttcgtct tttaaacgat tgcaggttac tacaatcaga
     9421 attattctta attattctgc aatcagagtt tccaaaccaa ctttcatcat atccgtgaaa
     9481 gtattttgac gctcttctgc tgtggtgttt tcctctggat taaccaagct atctgaaatc
     9541 gtcataatgg caagcgtttg aacgccaaac ttagcaccta ggtaataaag tgcagctgct
     9601 tccatttcta cagccttgac acctagttga cctaattcta agttgcgact aaacaatttt
     9661 ggagaataga aactatctga tgataaaaca ttgccaacat gcgttgtcat gttgagttct
     9721 ttggcgatat gataagcctt atctaaaaga tcaaaatcag cgatttgtgg caaatcgtac
     9781 tctggccagt caatattaat catgcgagag ttggtcgcgg ctgcttgcgc caaaaccaat
     9841 tcacggacat ggacatcctc attcaaagaa cctgctgttc ccacacgaat tagttttttc
     9901 acaccatatt cgttaatcaa ttcatgagca tagatagaga tggatggcat tcccatacct
     9961 gttcccatga cagaaacacg gtgacctttg taagtaccag tatagccaaa catattgcgg
    10021 acttcattga agcaaactgc gtcttcaaga aaattttcag cgataaattt agcacgaagc
    10081 ggatcaccag gaagcaaaat cttatcagcg atttcgcctg gttttgcgga aatatggata
    10141 gacatgtaat gtacctcttt gttctatttt cgaatgctta caactcagcc aagatagctt
    10201 tcaccaagcc tttgaagttg cccttgattt gctcggttac ttctacaact tcttcgtgat
    10261 tgagttcaga ttggaaacca gctgcaaagt ttgtgatagc agaaataccc aagactttca
    10321 tacctgagtg agctgccacg ataacttctg gtactgttga cataccgact gcgtgggcac
    10381 ccatagtctt gaaggccaag atttcagcag gtgtttcata ggtagggcct gtcacaccga
    10441 gatagacacc atcgtccaac ttgataccca atttttccgc aacggcatgg gctttttcac
    10501 ggtattcttt agtgtaggca ttggacatat ctgggaaacg tggaccaaat tcttccaagt
    10561 tttcgccaat caatggattt tggccagtca tgttgatgtg gtctgtaatg gccataaggg
    10621 tacctggacc gtagccaata ccacctgcgg cattagtaac aatcacgcct tcacaaccaa
    10681 gagctttcat gacacggact gggaaagtca cgacctccat aggattgcct tcgtagaagt
    10741 ggaatcgacc ttgaagagct aagaccttac gtcctgccaa atcaccgtac accaacttac
    10801 cagcatgacc aacaactgtt gacttacccc agtttggaat atctgcatag tcaatgacaa
    10861 tggcattttc aacttcttgg gccaattcac ccaagccaga acccaaaatc aaaccgaatt
    10921 caggttttac caaacctttt tcttccaaga acgtttttgt ttcattgatt tttgcaagta
    10981 aactcatatt ttctcctttt ataaataatc cgaccagtca tattctggca ttttttcttt
    11041 aatgatttca agcggacgac agaggaaggc tgtctgctct cgctcgataa tggggcgatt
    11101 gagagtggca ggctcttcca ctaaacgctc aaagatttct tcttctgaca aggctagtcg
    11161 ttcttcttcc ttcaatcgag taactgctga aggttgactc cccatttttt ccaagagagc
    11221 tgtcaattca gctcccctta atggcgtttc caaataatta acccacttga cttccatatc
    11281 ctgactggac agcaatattc ttaatttttt acacttgcta cagttaggat tgtaatagac
    11341 cgttagcttc tccattaaac caattgtgac aagaagcttt cgccaatcat ggctgtgtcc
    11401 actccgaagt tttccgcaac ggttgctgaa atgtccgcaa aatggcctac tggcaaaaca
    11461 ccgctacctt tgaaagcctt gctgtatgct aagagtggca catattcgcg tgtgtgatct
    11521 gttccagcat aggatggatc attgccgtgg tctgcagtaa tcaagagcag gtcatcttca
    11581 cgcatattgt caataatctc tggaatacgt gcatcaaact cttgcaaaca atcgcggtag
    11641 ccttcgatat tgcgacggtg accatacaca gcatcaaagt caactaagtt tgtgaaggag
    11701 aaaccttcct cgaagtctga taacttcaga gtatcaatca acacatcgac accgtgcatg
    11761 ttggacttgg tgtgacccat atcatttgta atacctgaac cattgaaaat atcactgatt
    11821 ttaccaactg agtaagttgg tacacccgca tctgccagtt tgttgagtac tgttgcctta
    11881 aaaggtgata ctgcatagtc gtgacggttg gcagttcgtg agaaattgcc tggctcacca
    11941 acgtaaggac gggcgatgat acggcctaga agagctgggc gttcaagcgt aatggaacga
    12001 gcgtattcac agatacggta caattcatcc agcggaataa tgtcttcgtg ggctgcaatc
    12061 tgaaggactg ggtctgcgga ggtatagaca attaactccc cagtttccat ttggcgaggg
    12121 ccaaagtcat caatgacagc tgtacctgaa tacggcttgt ttgcttcgcg gatgatttta
    12181 cgacctgaga attcttcgat ttttgtcaaa atctcttcag ggaaaccatc ccagaatgta
    12241 tcaaatggct cagtaatatt gagacccata atttcccagt gtccagtcat ggtatcctta
    12301 ccaagcgata cttcttccaa cttggtcacg taaccagttg gatggctttc agcaggaact
    12361 gtcgcaagcg ctgtatctcg agggatattt ccaagaccaa tcttggtcat attaggcacc
    12421 tcaagcccag ctttttcaga aatgtggccg agagtgtcag attctgtatc tgctacaccc
    12481 gcattgaaaa atttatctga gtctggtgct gcgccgattc cgacagagtc cataactacc
    12541 aagtgaacac gtttaaattt aggcataatt gtctccttta tttgatatag ttagattcaa
    12601 ccaaaatcag cgtccaacca agttgctaga ctggaaatta tcattaccac acctaaacat
    12661 tttaatgggg tgcaatactt atacagtcaa agctatcggc atcaaacttc ttaaccatca
    12721 aaactagata ctgttactcg cttcactgcc tgctgttcag ctatcgcttt ttcagtacct
    12781 attgtctgac tgctacgagg taaggggata gatttttgaa gagtctaaac gttcgtgttt
    12841 tcattatacc ataaaaaagc ctctcattgt accaaaatga gaagcgtttt caatttattt
    12901 cttttcgatg atgttcggtc cttctagtgt accaacaatt accttggaaa tcaatccaat
    12961 aaagagaccg tgttctacaa caccgacggt ccgatccagt tcttccgcca atgcatatgt
    13021 gtcttctatt ctgcgcaaat caaggtcaat gataaagttt tgcatatccg tgatatgttt
    13081 ttctccatta cgcatacgaa aacttggacg ataccctttt ttctcaaata gtcggaaaag
    13141 attttccgca ccgtactgta caacttccac cggcaacttg aaggctccta gggtttgaac
    13201 caccttagac tcatccacaa tccagatgca gtccttgcta ttgactgcta ccactttctc
    13261 catgagaagc gctgcgccac cgcctttaat cccattgaag tccccatcaa cctcatctgc
    13321 tccatccacc gtcaagtcca catattcgac ttcgtcgata ttttttaggg gaatcccaag
    13381 ggacgcagca tgttctgccg tagcatgaga ggtcgttacg cctgtgattt gtaacccttc
    13441 ttctgcaacc cggcggccaa tctcctgcac gaaatagtat gccgtcgaac cagtccccag
    13501 cccaacaatc ataccgtccg tcacaaactc cgccgcctta atcccaacct gctcttttag
    13561 gtttgtcatc tttgtctcct tttaaaaaac tgatacaact attatagcat gaagcaagca
    13621 agacaaaaca agaattttag aaaattgtgg gggagatgta ctatcgctta tgattacgat
    13681 aatataaatc ttgagagtac aaaaactaca aatgttatgg ggataactac cagaaggttt
    13741 tactgttact taaatcttga gagtacaaaa acttaactga aaattagtag cgtaaggaag
    13801 tggttttact gttacttaaa tcttgagagt acaaaaacga caaggtcgaa aagaaataca
    13861 ttgatgttgt tttactgtta cttaaatctt gagagtacaa aaaccctgca gatgttaacc
    13921 cactcaataa tccgttttac tgttacttaa atcttgagag tacaaaaact ggccacgatg
    13981 cggtccaaag agataattag ttttactgtt acttaaatct tgagagtaca aaaaccagcc
    14041 tcttcgtatt tggatagtaa agaatgtttt actgttactt aaatcttgag agtacaaaaa
    14101 cacatcattt gtttatcggg acccaaccaa tgttttactg ttacttaaat cttgagagta
    14161 caaaaaccag tattaattgg gatattattt atcttaggtt ttactgttac ttaaatcttg
    14221 agagtacaaa aaccaacatg gttttgatcc cacgtttggc cagttttact gttacttaaa
    14281 tcttgagagt acaaaaacgc cttttttgac ctttgacacg atatgctggt tttactgtta
    14341 cttaaatctt gagagtacaa aaacctcaaa
//
LOCUS       DAZQCJ010000044        14192 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000044 DAZQCJ010000000
VERSION     DAZQCJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14192
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.045"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            169..2400
                     /gene="gshAB"
                     /locus_tag="ACHU66_001212"
     CDS             169..2400
                     /gene="gshAB"
                     /locus_tag="ACHU66_001212"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFR3389500.1"
                     /translation="MLQKLSPNSPILQATFGIERESLRINSNHRVAQTPHPHKLGSRS
                     FHPYIQTDYSEPQLELITPIAQSTKEARRLLGAITDVAARSMDKQEYLWPLSMPPVIS
                     EEEIQIAQLDSDYEYQYRVGLGERYGKLVQSMSGIHYNFELGKDLTQQLFELSKETDF
                     IAFKNTLYLKLAQNFLNYRWLLTYLYGASSLAEKGFLTTEVGCVRSIRNSKYGYVNSD
                     DVHISFSSLEQYVADIEQAVQSGQLSAEKEFYSSVRLRGAKTSRDYLSKGISYLEFRS
                     FDLNPYDPLAISQETLDTVHLFILSLLWLDQLTDVDNTLAKADKLNNLIALSHPHTPL
                     PNDADATPILTAMKAIVLHFGLDDYYGQLIAHVEAALQDPRLTLSGKIAEQIEDGSLE
                     KFGQQQGRLFHDYAWTAPYALKGYENMELSTQMILFDAIQLGLNVEILDEEDQFLKLW
                     HGDHVEYIKNGNMTSKDNYVIPLAMANKVVTKKILDQAGFPVPAGAEFANKTDTLRYY
                     GQVASSAIVVKPKSTNFGLGISIFQEPASLADYEKALDIAFSEDSHVLVEEFVAGTEY
                     RFFVLDGKCEAVLLRVAANVVGDGSSTIRELVDQKNQDPLRGRDHRSPLEIINLGDIE
                     LLMLEQQGYTPDTVLPEGVQAFLRGNSNISTGGDSIDMTDQMDESYKQLAADMATAMG
                     AWACGVDLIIPDRTKPASKDKPNCTCIELNFNPAMYLHTYTYAGPGQSITPKILKKLF
                     PEL"
     gene            2957..6076
                     /locus_tag="ACHU66_001213"
     CDS             2957..6076
                     /locus_tag="ACHU66_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6359 domain-containing protein"
                     /protein_id="HFR3389501.1"
                     /translation="MKIRNRSLFYTVGSVAVTAGLLFSLATSPIPSVHATEVAIENYP
                     SLAITKSEVTIQGYLIAPLNSSGTAFDATNKTNLALGASMDTAAADTIPIQLKEPLRS
                     QFNLVDHPELVGKLVRITGTSDTYMKRAGIKPATAIEIVDSSSTNVQPPTSENTATKP
                     SDLVSTPIATVRSGAQGTEYTVSGKIISLVNGWGGNGFYLQGSDGAGIYIYPGAALGY
                     QPGDTVQLTGTLGEYKGELQLTTVSNHKAISENFNTPITETNIAQLATQAQATLVSLK
                     NLTVGEIQSDSYQNSTFTVTDSEGQTVDVRLDSRTGIKTADLLNRINKGDKINLTAIL
                     STYNGKIQLKPFDLSHFEVIEKATTEAGLGKTEAVTVGRIQGASHQSPLVNQSVMLKN
                     VVVTYVTSANNFYVQDVTPDGDIKTSDGINIFTDKLKTNVKVGDLVTIAGRVEEYQGR
                     GYAERDKTDLTITQIRATEVTVNGTAPVPSPIVLGLDRTIPADIIDNDGLAQFDPEQD
                     ALDFWESVEGMVVAVDDAKILGPLKNKEIYVTPATSQLPLNNVGGVNLRPEGNNTNII
                     PLLLKNGKQIVKSGDYFTGRIAGPVTYSYTNYKVYVDDSTLPTLHEGATKPETTTIIP
                     NDDKLTIASYNIENFSADSKSTSDAKVQRIAKSFVSDLHSPDVIGLIEVQDNNGATND
                     GTTDASKSAERLIAAIQAAGGPTYTYVDIAPENNKDGGQEGGNIRVGFLYNSKRVSLS
                     DKPIGTATQAVAWENGELNLSLGRIDPTNPAWAAVRKTLAAEFVFKGEKVVVLANHLN
                     SKRGDNGLYGKIQPVSFKSEEKRHSLAQIITDFTKAGLTQNPNANIVMLGDFNDYEFT
                     KTIEILETGGMANLVSRHDASDRFSYFYNGNNQSLDNMLVSTNLLDRYAFDMVHVNSA
                     FMEEHGRASDHDPLLVQLDVTKAQEPTQPEPSDKQTDDSGTVNNSDDNGTTNNNKPTN
                     LSTSNQTAVNADDRSAATDKGQITVTPTANNSQKKILPKTGGETSFVLITIGLVFLSA
                     CLVKKQKES"
     gene            6156..7022
                     /gene="hslO"
                     /locus_tag="ACHU66_001214"
     CDS             6156..7022
                     /gene="hslO"
                     /locus_tag="ACHU66_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR3389502.1"
                     /translation="MDKIIKTLSKSGHFRAFVLDSTETVKTAQEKHDTMASSTVALGR
                     TLIANQILAANEKGDTKITLKILASGAVGAIISVANTKGQVKGYIQNPDLDYKRTATG
                     EVIVGPLVGNGQFLVITDYGTGHPYNSMTPLISGEIGEDFAYFLTDSQQTPSAVGLNV
                     LLDEEDKVKVAGGFLLQVLPGATEAEIARFEKRIQEMPAISSLLASENHIEALLSAIY
                     GDDDFKRLSEEEIGFVCDCSKDRFLDALASLPKTDLQEMKEEDKGVDITCQFCQTHYH
                     FDENDLEELING"
     gene            7015..8019
                     /gene="dusB"
                     /locus_tag="ACHU66_001215"
     CDS             7015..8019
                     /gene="dusB"
                     /locus_tag="ACHU66_001215"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR3389503.1"
                     /translation="MANLNTPFMIGDVEIPNRCVLAPMAGVTNSAFRTIAKEMGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDDNEYPMSIQLFGGDAEGLKRAADFIQTNTKANIV
                     DINMGCPVNKVVKNEAGAKWLKDPDKIYHIIKEVTSVLDIPLTVKMRTGWNNTDLAVE
                     NALAAESAGVSALAMHGRTREQMYTGTVDLETLTKVAGSLTKIPFIANGDIRNVEDAR
                     QRIEEVGADAVMVGRTAMGNPYIFNQINHYLETGEVLPDLSFEDKLEVAFDHLTRLTN
                     LKGESIAVREFRGLAPHYLRGSAGAAKIRGAVARAETIEQVQELFNQAREAYQERIAK
                     "
     gene            complement(8120..8611)
                     /locus_tag="ACHU66_001216"
     CDS             complement(8120..8611)
                     /locus_tag="ACHU66_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFR3389504.1"
                     /translation="MNQTIAVVFGTFAPMHKGHLDLIERAKLACGQVCVVVSGYDRDR
                     GDRIGLDLTKRFQFAQEQFKEDDFVEVRALDETDLPPYPDGWDLWLDRLLGLLDLSEH
                     QLPVFYVSEEEYAQELHSRGYQAHFSPRKFGISATLIREQPQQYQDYIAPAFLAFFGK
                     ETM"
     gene            complement(8729..11182)
                     /locus_tag="ACHU66_001217"
     CDS             complement(8729..11182)
                     /locus_tag="ACHU66_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFR3389505.1"
                     /translation="MKISTGLQRVFEDAQLVAQRYACDYLETWHVLLSFVINHDTVAG
                     AVLAEYPISISDYEHATFVVTDKVYREELDSFRILPSSKRLDETASFAKKIAEVVKAK
                     ELGTEHLFMAMLLDKRSTASQILDKVGFCFEDSDDKFRFLDLRKNLEARAGFTKEDLK
                     AIRSVMKGGKAKPTNMGQMMGMPPAPQSGGLEDYTRDLTALAREGKIEPVIGRDAEIA
                     RMIQILSRKTKNNPVLVGDAGVGKTALALGLAQRVAAGDVPVSLAKMRVLELDLMNVI
                     AGTRFRGDFEERMNNIINDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALA
                     RGTLRTVGATTQTEYQKHIEKDAALVRRFAKVTIEEPTVEDSIAILTGLKGTFEKYHR
                     VRIGQAAVETAVTYAKRYLTSKNLPDSAIDLLDEASATVQNRVKGQGEETGLTSIDKA
                     LMDKKYKTVSKLLIETKEDAEASQNYDLEVTEEDVLETLSRLSGIPVAKLSQSDTKKY
                     LNLEAELHKRVIGQEEAISAVSRAIRRNQSGIRTGRRPIGSFMFLGPTGVGKTELAKA
                     LAEVLFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLL
                     FDEVEKAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGF
                     GALDLSKDHKEVEKRIFEELKKAYRPEFINRIDEKVVFHSLTETHMQDVVKVMIKPLL
                     AVTAEKGITLKLQPSALKLLAKQGYDPEMGARPLRRLLQTKLEDPLAEMLLRGDLTTS
                     STLKVGVKGEELKFDVVKG"
     gene            complement(11186..11638)
                     /locus_tag="ACHU66_001218"
     CDS             complement(11186..11638)
                     /locus_tag="ACHU66_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFR3389506.1"
                     /translation="MKNTSDYIEEHIKALLEQVSVAELKRSELASRFEVVPSQINYVI
                     KTRFTASRGYIVESKRGGGGYIRIGRITFSDKHELVHDLLNNMGEQLSATVYADILQL
                     LFDEGLMTEREGNLFLFTGSDEILGKDASTIRARMMRQILRRLDRKEE"
     gene            11806..12165
                     /locus_tag="ACHU66_001219"
     CDS             11806..12165
                     /locus_tag="ACHU66_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HFR3389507.1"
                     /translation="MFDTNHFFEDIKDFKAVSPKEASELLAAKDGAILFLGRDTCPYC
                     RRFAPKLAAVAAAQNWTVHFLHTQDPAYSAQEIAALREQYHVPTVSGLLVAKASGVDV
                     RCDSSLTEDEILAFVMA"
     gene            complement(12237..14105)
                     /locus_tag="ACHU66_001220"
     CDS             complement(12237..14105)
                     /locus_tag="ACHU66_001220"
                     /inference="COORDINATES: protein motif:HMM:NF024296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFR3389508.1"
                     /translation="MTKMKKILAFILLPVMILFSGYLGASSHVYGDETTNVITKMYFT
                     DKDGNELTPPNVEQWQQFRLNADFDIKNNAVKEGDVIEIELPSELIFNQSSDFQITDK
                     EGNVVANATITPEPEKKIKLTYTKYVEEHSDIKGSFYFYVRVDHRVIQQKKEIPLDIT
                     VQGKIIHAGKIQFQGIGTPDKRDIQKSGWSDSKNPQDAWFIIAINRSGQSMKDVKVVD
                     VLKSKQLEYIPDTLRVEKGNWSFKAGDWVLDNIETITDQVTLTFAPDGKSFSIELPDL
                     APTDGIQIIYKTKAPYVPVDGEEFSNEATLITTNGIDQTVTSGYTYLAAGGKAEGYVF
                     TIKVKKTDEAGAPLADVEFDVIRNRTGQSVGKITTDDQGEGSISNLLRDEYTLKEIKT
                     PDGYLPIEDMVINPAEFDGVTKSVLKEIVNKKKPAGPVKVVLKATKTLEGRALADQEF
                     EFTLLKKDDGSVVSTAKNDGTGVVTFDELTFDAPGTYKYTVKEVLGTDAGIDYDEVEY
                     DVIVTVTDNGGPTLVATVDKSEQEVNFINKVITTTTTTTTTTTTSEETTTTPETPAST
                     TTVETTVPEVPASTTTTGKILPKTGEKSGLWTSVLGVIFLVVGAGAVIFYRQSKEA"
BASE COUNT         4105 a         3474 c         2767 g         3846 t
ORIGIN      
        1 gaggtagaca tgtgtaaata tgcttttggc agagaaatcc gaagtctttg tttactttca
       61 gtgattttca gtacattttt caccagaaac gtagcagaaa tttgtatttg ctctccaatc
      121 tagataaaat tggaataaga ctttgacaga aatataaagg acttagatat gttacaaaaa
      181 ttatcaccaa acagccccat tcttcaggct acttttggaa ttgagcgcga atcacttcgc
      241 atcaattcaa accacagagt tgcacaaact ccccatcctc ataaactggg ttctcgtagc
      301 ttccacccct atatccagac agactacagc gagccacagt tagagttgat tacaccaatc
      361 gctcagtcga cgaaggaagc gcgtcgtcta ctgggagcca ttacagatgt tgccgcacgt
      421 tctatggata agcaagaata cctctggccc ctatccatgc cacctgtgat ttcagaagaa
      481 gaaatccaaa ttgctcaact agatagcgac tatgagtacc aatatcgtgt cggtttgggg
      541 gaacgctatg gcaagctcgt ccagtctatg tccggcatcc attataattt tgaattagga
      601 aaagacttaa cccaacaact ttttgaatta agcaaggaaa cagactttat cgcattcaaa
      661 aataccctct acctcaaatt agcacaaaac ttcctcaact atcgttggct cttgacctac
      721 ctatatggag caagtagcct agccgaaaaa ggattcctaa ccacggaagt cggttgcgtc
      781 cgttccattc gtaattccaa gtacggctat gtcaactccg atgatgttca tatttccttt
      841 tcttctctcg aacagtacgt ggccgacatc gaacaagctg tccaatctgg tcaactatcc
      901 gctgagaagg aattttactc ttccgtccga ctacgtggag caaagactag ccgtgactac
      961 ctcagcaagg ggatttctta tttagaattc cgttccttcg accttaaccc ctatgacccg
     1021 cttgctatta gtcaagaaac ccttgataca gtccacctct ttatcttatc tcttctttgg
     1081 ctagaccaac tgacagatgt tgataacacc ctagcaaaag cagataaact taacaatctc
     1141 atcgccctca gtcaccccca cacacctcta cctaacgatg ccgatgccac gccaatcttg
     1201 acggccatga aagccatagt tctacacttt ggactggatg actactatgg ccagctcatt
     1261 gcacatgtag aggcagcact tcaagaccct cgtttgaccc tttctggaaa aatagcagag
     1321 cagattgaag atgggtctct agaaaagttc ggccagcagc aaggccgact cttccatgat
     1381 tatgcttgga cagcccctta cgcccttaag ggctatgaaa acatggaact ctccacccag
     1441 atgattctct ttgacgccat tcagctgggc ttgaatgtgg agattttaga cgaggaagac
     1501 cagtttctca aactctggca tggtgaccat gtcgagtaca tcaaaaatgg taacatgacc
     1561 tccaaggaca actacgtcat tcctctcgcc atggccaaca aggtcgtgac caagaaaatc
     1621 ttggaccagg ctggttttcc agtacctgcg ggagctgaat ttgcaaacaa gacagacacc
     1681 ctgcggtatt acggacaggt agccagctct gctattgttg tcaaaccaaa gtccactaac
     1741 ttcggtctcg gcatctctat cttccaagaa ccagccagcc ttgcggacta tgaaaaagcc
     1801 cttgacatcg ccttttcaga agacagccat gtactagtgg aggaattcgt ggcaggaacc
     1861 gagtaccgtt tcttcgtcct ggacggcaag tgcgaggctg tcctgctccg cgtcgcagcc
     1921 aatgtcgtgg gggacggtag ctcaaccatc cgtgaattgg tggaccaaaa aaaccaagat
     1981 ccactgcgtg ggcgtgacca ccgttcaccg ctggaaatca ttaacttggg cgacatcgaa
     2041 ctgcttatgt tggagcaaca aggctacaca cccgatacag ttttgccaga gggcgttcaa
     2101 gcctttctgc gtggcaattc caatatctca acaggtggtg actccatcga tatgaccgac
     2161 cagatggacg agtcctacaa gcaactggcc gctgatatgg cgactgctat gggagcttgg
     2221 gcttgcggtg tggacttgat tatccccgac cgcaccaagc cagccagcaa agacaagccc
     2281 aactgcacct gtattgagct caacttcaac cctgccatgt acctgcacac ctatacttac
     2341 gcaggacctg gacagagcat tacgccgaag attttgaaaa aattgtttcc agagctatag
     2401 ttttccattt tccttacttt gttagtactt acttcaaaga ttcgtggagc ttttgaaggc
     2461 tggagatagg gcaaagcgag ttcgcctcaa tagcggcagg ttgctagaac attctaggtt
     2521 ggaaataaga cttgcctctc tataaagtac ggcaaagcga gttcaaacaa accagtggat
     2581 tgtttgaagt gggaaagcca gaaacggggt tcctcttgcg tctaagtaaa aaagtcaact
     2641 aaaggactat atatctgctt tgcccctttg ttccttcacg aaaactccca actaatgtta
     2701 aacaaaacga ataagacacc gagataatca tttatatctc ggtgcttttt tgtgaactaa
     2761 ttcccatcac tcaaccgttt tgttcgcctt ttattttgtt ttccaattta tttttattat
     2821 ttcatttgat tttttgaaaa tatccgtatt atatctaaaa aaagtattcc aaacgactca
     2881 ttttctgtta aactatatgt atataacaac tgcagacgtt tttatgacgt ccatatataa
     2941 caaaaaggag ttttatatga agattagaaa ccgttctctc ttctataccg taggttctgt
     3001 agcggtaaca gccggactct tgtttagtct tgctacttcg cccataccat ctgtgcacgc
     3061 tactgaagtg gcgatagaga attatccgtc acttgcaata accaagtcgg aagtcacgat
     3121 acaaggctat cttattgcac cacttaatag tagcggaact gcttttgacg ctactaacaa
     3181 aacaaatcta gcccttggtg caagcatgga tactgcggcc gccgacacta tccctattca
     3241 actaaaagaa cctcttcgta gccaattcaa cctcgttgat catcccgaac tagtcggaaa
     3301 attagttcgt atcacaggaa ctagcgatac atacatgaag cgagctggga tcaagccagc
     3361 tacagcgatt gaaattgtag attcatcttc tactaatgtt cagccaccaa caagtgaaaa
     3421 tacagctacc aaaccaagtg accttgtttc tacaccgatt gctactgttc gttccggagc
     3481 acaaggaaca gagtacacag tttctggtaa aattattagc ctagtaaacg gttggggagg
     3541 aaatggtttt tacctccagg gttctgacgg tgctggtatc tatatctacc caggggctgc
     3601 cttgggctat cagcctggtg atacagtcca attaacagga acattgggtg agtataaagg
     3661 cgaactccaa ctaaccacag ttagcaacca caaggctatc tctgagaatt tcaacactcc
     3721 tattacagaa acaaacatcg ctcagttagc aacgcaagca caggcaacac tagtttctct
     3781 aaaaaacttg actgtcggag agattcaaag cgatagctat caaaattcta cctttaccgt
     3841 aactgattcc gagggacaaa ctgtggatgt gcgtttagat agtcgaacag gcatcaaaac
     3901 ggcggatctt ttaaaccgga tcaacaaggg agataagatt aatctaaccg ctattttatc
     3961 cacctataat ggaaaaatac aattaaaacc atttgacctc tctcattttg aagtcattga
     4021 aaaggcaacg acggaagcag gactaggaaa gaccgaagct gtaacagtcg gtcgcattca
     4081 aggtgcaagt caccagtcac ctctggtgaa tcaatctgtt atgcttaaaa atgtcgtagt
     4141 aacctacgtt acctctgcca ataacttcta cgtgcaagat gtcacaccag atggagacat
     4201 caaaacatct gacggcatta acatctttac tgacaaatta aaaacaaatg tcaaggtagg
     4261 agatcttgtc actatcgcag gacgagtaga agaataccag ggcagagggt atgctgaacg
     4321 tgacaaaaca gacttaacca tcacacaaat tcgagctact gaagtaactg tgaatgggac
     4381 tgcacctgta ccatctccta tagtccttgg tcttgatcgg accatcccag cagatattat
     4441 tgacaacgac ggtttggctc aatttgatcc agaacaagat gcgctcgatt tctgggaatc
     4501 cgttgaaggc atggtggttg ccgtggatga tgcaaaaatc cttggcccac taaaaaacaa
     4561 ggaaatctat gtcactcctg ccactagtca acttccttta aataacgttg gaggcgtcaa
     4621 ccttcgccct gaagggaata ataccaatat cattccactc cttttgaaaa atggaaaaca
     4681 gatcgtcaaa tcaggagact atttcaccgg tcgtatcgct ggaccagtta cctactctta
     4741 caccaattat aaagtctatg tagatgatag cacccttcca accttgcacg aaggagctac
     4801 aaaaccagaa acaacaacca ttatcccaaa tgatgacaag ctgactatcg cttcttataa
     4861 cattgaaaac ttttctgctg acagtaagtc aacatcagac gctaaagtcc aacgcattgc
     4921 caaatccttt gtttcagacc tacattctcc agatgttatc ggattgattg aagtgcagga
     4981 caataacggt gctacaaatg acggcacaac tgatgctagt aagagtgctg agcgcctaat
     5041 tgcagccatt caagcagctg gcggaccaac ctatacctat gtggatattg ctcctgaaaa
     5101 taacaaagac ggcggacagg aaggtggcaa tattcgcgtc ggcttcctct acaactcaaa
     5161 acgtgtcagc ctatctgaca aaccaatcgg taccgcaaca caggcagttg catgggaaaa
     5221 cggcgaactc aatctcagcc tgggccgaat cgacccaacc aaccctgctt gggcagctgt
     5281 ccgcaaaaca cttgcagctg aattcgtctt caaaggcgag aaagtcgttg tccttgccaa
     5341 ccatctcaac tcaaaacgcg gtgacaatgg tctctacgga aaaatccaac ctgtcagctt
     5401 caagtccgaa gaaaaacgcc actcactagc acaaattatc actgacttta caaaagcagg
     5461 tttaactcaa aatccaaatg ctaatatcgt tatgctaggc gacttcaatg actatgaatt
     5521 tactaaaacc atagaaattt tagaaacggg aggaatggct aacctcgtta gtcgtcacga
     5581 tgcatctgat cgcttctctt acttctataa cggaaacaat cagtctcttg acaatatgct
     5641 agtatcaact aacttgttgg accgctatgc ctttgatatg gtccatgtca attcagcctt
     5701 tatggaagaa catggcagag cttctgacca cgatccgcta ttggtacaac tggatgtaac
     5761 aaaggcacaa gaaccaactc aaccagaacc aagcgataag cagacagatg actcaggaac
     5821 agtcaataat agtgatgaca atggtacgac caacaataac aagccaacaa atctttcaac
     5881 ttctaatcaa acagctgtaa atgctgatga ccgttctgcc gctacagaca agggacaaat
     5941 aaccgtgacc cctactgcca acaacagtca aaagaaaatc cttccaaaga caggaggaga
     6001 aacaagcttt gtacttatca caatcggtct cgtattcttg agcgcctgcc ttgtcaaaaa
     6061 acaaaaagaa tcctaatcat tttttgagct tgaaagcatg actttcaggc tcatttttct
     6121 actgctccac ctccacttgt gctataatta aagttatgga taaaattatt aaaacactat
     6181 caaagagcgg gcatttccgt gcctttgtgc tagatagcac agaaaccgtg aaaacagccc
     6241 aagaaaaaca cgacaccatg gcgtcgtcca ccgttgctct ggggcgcacc ctcattgcca
     6301 atcagatttt ggctgccaat gaaaaaggcg acaccaagat caccctgaaa atcttggcca
     6361 gcggtgctgt tggggctatc atctccgttg ccaataccaa aggtcaggtc aagggctaca
     6421 ttcaaaatcc agacttggat tacaaacgga cagcgactgg cgaagtcatc gttgggcctc
     6481 ttgtagggaa cggacaattc ctggttatca cagactacgg aacaggtcat ccttacaact
     6541 ccatgacacc cttgatttct ggtgaaatcg gtgaagactt tgcctacttc ctaacagaca
     6601 gccagcagac accgtctgcg gtggggctga acgtcttgtt ggacgaagaa gacaaggtca
     6661 aggtagctgg tggcttcttg ttgcaagttc tgccaggtgc aaccgaggct gaaattgccc
     6721 gtttcgagaa acgcatccaa gaaatgcctg ccatttcgag tttgctggct tctgaaaacc
     6781 atatagaagc tctgctatcc gctatttatg gcgacgacga cttcaaacga ctgtcagaag
     6841 aagaaatcgg ttttgtctgc gactgctcta aagaccgctt ccttgatgct ctagctagtc
     6901 tgccaaaaac cgacttgcaa gagatgaagg aagaagacaa gggcgtggac atcacctgtc
     6961 aattctgcca gacccattac cactttgacg aaaacgattt ggaggaactc atcaatggct
     7021 aatctcaaca cccctttcat gattggtgat gtggaaatcc ccaatcgctg cgtgctggcc
     7081 ccaatggccg gcgtgaccaa ctcggccttc cgcaccattg ccaaggaaat gggcgcaggc
     7141 ctggtcgtta tggaaatgat ttctgaaaaa ggccttctct acaacaacga gaagaccctc
     7201 catatgctcc atattgacga caacgaatac cctatgtcca tccagctttt tggcggcgat
     7261 gccgaaggac tgaaacgggc ggccgacttc atccaaacca acaccaaggc taatatcgta
     7321 gatattaaca tgggctgccc tgtcaacaag gtcgttaaaa acgaagctgg tgccaagtgg
     7381 ctcaaggacc ccgacaagat ttaccacatc atcaaggaag tgacctcagt cctcgatatt
     7441 cccttgactg ttaaaatgcg gacgggttgg aacaataccg acctagcggt cgaaaatgcc
     7501 ctggccgcag aaagtgccgg tgtgtctgcc ctggccatgc acggacggac ccgcgagcag
     7561 atgtacacag gaaccgttga cctggaaacc ctcaccaagg tggctggcag cctgaccaag
     7621 attcctttca tcgcaaacgg cgacatccgc aatgtcgaag atgctcgtca acgtatcgag
     7681 gaagtcggag ctgatgccgt catggtcgga cggactgcca tgggcaatcc ttatatcttc
     7741 aaccaaatca accactatct tgaaactgga gaagtcctac ccgacctctc ctttgaagat
     7801 aaactcgaag tcgccttcga ccacctgacc cgattgacca atctaaaagg cgaatccatc
     7861 gccgtacgtg aattccgtgg tcttgcccca cactacctcc gtggttcagc aggtgctgcc
     7921 aagatccgtg gcgccgttgc acgagcagag acgattgaac aggtccaaga actctttaac
     7981 caagctagag aagcttatca agaacggatt gctaaataag aaactctgag aagtttgata
     8041 cttgccaacc atcaaacata tataaaaacg cataaaacca aggttataac ctgattttat
     8101 gcgttttttc tatactgttt tacatagttt cctttccaaa aaaggcaaga aaggctggag
     8161 caatgtaatc ctgatattgt tgaggttgtt cacgaatgag ggtcgctgaa ataccaaatt
     8221 ttctaggact gaaatgcgct tgatagcctc gactgtgcag ttcctgagcg tattcttcct
     8281 cagacacata gaaaactggt agttgatgtt ctgacaagtc caataaacca agcaggcggt
     8341 ctagccaaag gtcccaacca tctggatagg gaggcaagtc cgtttcatcc agggcacgaa
     8401 cttcgacaaa atcatcttcc ttaaactgct cttgagcaaa ttgaaatcgt ttggtcaggt
     8461 ctaaacctat cctatcccca cgatccctat catagcctga aaccaccaca caaacctgac
     8521 cacaggccaa ctttgctcgc tcgatcaaat ccaaatgtcc cttgtgcatg ggggcaaaag
     8581 ttccgaaaac aacagctatg gtttgattca tagatttcct ttctacttgc taaagaggcg
     8641 agcaagctca cctctccaac tatttcatcc ttttttcaaa acggatgaac aattctgcat
     8701 ctggaaagga tagcaacaca agcactcctt atccctttac cacatcaaac ttgagctctt
     8761 cacccttgac cccaaccttc aaggtcgaac tagtagtcaa gtcaccacgg agtaacattt
     8821 ctgccaacgg atcttccaat ttggtttgta agagcctacg tagcggacga gcccccattt
     8881 ctgggtcata accttgttta gccaataact taagggctga cggttgcagt ttaagagtaa
     8941 ttcctttctc agctgttaca gccaacaaag gtttaatcat gaccttgaca acatcctgca
     9001 tatgagtttc cgttaggcta tggaagacaa ctttctcgtc aatacggttg ataaattctg
     9061 gacgataggc ttttttcaat tcctcaaaaa tgcgtttctc cacttccttg tggtccttag
     9121 ataaatcaag ggcaccaaaa ccaactgtct tatcatcacg aagagctgtt gctccaaggt
     9181 ttgacgtcat gataataata gtatttgaga aatcaacctt acgtccctta ctgtccgtca
     9241 gaacaccatc atccaagacc tgcaagagaa cattgaaaat atctggatga gccttttcaa
     9301 cctcatcaaa aagaagaaca gaataaggtt tgttacgaac tttttctgtc aattcgccac
     9361 cttcttcata gcccacgtaa cctggaggcg ctccattgag acggctggcc gcaaatttct
     9421 ccatatactc ggacatatca aaacggataa gagcagattc atcatcaaag agaacctctg
     9481 ccaaggcttt agcaagctct gtcttaccga caccagttgg tcccaagaac ataaaggaac
     9541 caatcggtct gcgacctgta cgaatccctg actgattacg gcgaatagct ctgctaactg
     9601 cagaaatagc ttcttcctgc ccaatcacac gcttgtgcaa ttctgcttcc agattcagat
     9661 acttcttagt atctgattgg ctaagcttgg ctactgggat acctgacaag cggctaagtg
     9721 tttctaaaac atcttcttct gttacttcaa ggtcatagtt ttgactagcc tcggcatctt
     9781 ctttcgtttc aatcaaaagc ttgctgacag tcttatattt tttatccatc aaggccttgt
     9841 ctatgctcgt cagtcctgtt tcctcaccct gccctttcac tcgattttgt accgttgcac
     9901 tggcttcatc cagcaagtca atggcagaat ctggcaggtt tttactggtc aaataccgct
     9961 tagcgtaggt cacagcagtc tctacagctg cttgtccaat acggaccctg tggtattttt
    10021 caaaagtacc ttttaaacca gtcaaaatgg caatgctatc ttccactgtc ggctcttcaa
    10081 tggtcacttt ggcaaaacga cgaaccaggg ctgcgtcttt ctcaatatgc ttttggtact
    10141 ctgtctgagt cgtcgctccg accgtacgga gggtgccccg agcaagagct ggtttgagga
    10201 tattggctgc atccaaggtc gaatcaatcc ctgagccaga acccataatg gtatgcaact
    10261 catcgatgaa aaggatgacc tgaccatctt cctcgatgtc gttgatgata ttattcatcc
    10321 gctcttcgaa atcaccacgg aaacgagttc cagcgataac gttcatcaag tcaagttcca
    10381 aaacccgcat cttagccaag ctgacaggga catcacctgc agcaacacgt tgagcaagac
    10441 ccaaagcaag cgcagttttt ccgacaccag catcaccaac tagaactggg ttattcttgg
    10501 ttttacgtga tagaatttgg atcatccgtg caatctcagc gtctcgaccg atgacaggtt
    10561 caatcttccc ttcacgcgcc aaagctgtca aatcacgagt gtagtcttct aatccaccac
    10621 tttgaggtgc tgggggcatt cccatcatct gtcccatatt agtaggtttg gctttgccac
    10681 ccttcatcac actgcggata gctttaaggt cctctttggt aaatcccgca cgtgcctcca
    10741 agtttttacg caaatccaag aaacgaaact tatcatctga gtcttcaaaa caaaaaccta
    10801 ccttgtccaa aatctgacta gctgttgacc gcttgtccaa caacatagcc ataaacaaat
    10861 gttccgttcc cagctcctta gccttgacga cttctgcaat tttcttggca aaactagccg
    10921 tttcatccaa acgtttagaa gatggtaaaa tacgaaaact atccaactct tcacgataga
    10981 ccttgtcggt cacaacaaaa gttgcatgtt catagtcaga aattgaaata ggatattctg
    11041 ccaaaacagc gccagcgact gtatcatggt tgataacgaa agacaagaga acatgccagg
    11101 tttccaagta atcacaggca taacgttgag ccactaactg ggcatcttca aaaacccgct
    11161 gtaaaccagt tgaaattttc atgaattatt cctctttcct atctaaacgt cgtagtattt
    11221 gacgcatcat gcgtgctcga atagtacttg catcttttcc taatatttca tcagatcctg
    11281 tgaacaaaaa gagattgcct tctcgttctg tcatcaatcc ttcatcaaaa aggagctgta
    11341 aaatatccgc ataaaccgta gcagacaact gctcacccat attgtttaac aaatcatgga
    11401 ccagttcgtg cttatccgaa aaggtaatcc gcccaattcg gatataacca ccaccaccac
    11461 gcttactctc aacaatatag ccacggctgg ctgtaaaacg ggttttaatg acgtagttaa
    11521 tctgactcgg taccacttca aatcggctcg caagttcgct ccgttttagc tccgctacac
    11581 tcacctgttc caagagagcc ttgatatgct cctcgatata atccgacgtg tttttcatac
    11641 acttcccttt ctaactagac tgactatttt tgactattgt cttattatac cgaaaaatca
    11701 cccatttgga aagcaaagac accgtttcta caacgtatca attttttgct ttctcccgtc
    11761 aaatagctta tactaaacct attacaatga aatggagaat accccatgtt tgataccaat
    11821 catttttttg aagatatcaa agatttcaaa gctgtctcac caaaggaagc aagcgagctt
    11881 ctcgctgcta aagacggtgc catcctattc ctaggtcgcg acacttgccc atactgccgt
    11941 cgcttcgcac caaaattagc tgccgtagct gctgctcaaa actggaccgt tcacttcttg
    12001 catacacaag atcccgccta ttctgcacaa gaaatcgctg cactacgtga acaataccat
    12061 gttccaactg tatcaggcct tctcgttgcg aaagcaagcg gcgttgacgt tcgctgtgat
    12121 agcagcctaa cagaagacga aattctcgcc tttgtaatgg cctaatcgtt taaaatagta
    12181 ccaaaaaaga gctgagtaat gactcggctc ttttcatgtt ggagatgata ggactattac
    12241 gcttctttcg attgacgata gaagataaca gctcctgcac cgactactag gaatataaca
    12301 ccaaggacag atgtccaaag tcctgacttc tcaccagtct taggaagaat ttttcctgtt
    12361 gtagtggttg aagctggtac ttctggaact gttgtttcaa ctgtagtagt tgaagccggt
    12421 gtttcaggag tagtcgttgt ttcttctgaa gttgtagttg tagtcgtcgt tgttgtagtt
    12481 gttgtgatca ctttattgat aaagttcact tcttgttctg atttatcaac tgtcgcaaca
    12541 agtgttggac caccgttatc tgtaacagtt acaataacgt catactctac ttcatcatag
    12601 tcgatacctg catctgtacc aagtacttct tttacagtgt acttgtatgt acctggtgca
    12661 tcaaatgtca attcatcgaa tgtaaccaca ccagtgccat cgtttttagc agttgaaaca
    12721 actgaaccgt cgtctttctt caagagagtg aattcaaact cttgatctgc aagtgcacgg
    12781 ccttccaatg ttttcgttgc tttcaaaaca actttaactg gaccagctgg ttttttcttg
    12841 ttgacaattt ctttaagaac actcttcgta acaccatcaa attccgctgg attgatgacc
    12901 atatcttcga ttggaaggta accatctggc gtcttgattt ctttaagagt gtattcatct
    12961 cttagaagat tgctaatgct accttcacct tgatcgtctg tagtaatttt accaacactt
    13021 tggcctgtac ggttacggat aacatcgaac tcaacatctg ccaatggagc acctgcttca
    13081 tcggtcttct taactttaat agtaaataca taaccttcgg ctttaccacc tgctgcaagg
    13141 taagtatatc ccgatgtaac ggtttgatca ataccattgg tagtaatcaa tgttgcttca
    13201 ttgctaaact cttctccatc aacagggaca tacggtgctt ttgttttata aataatttga
    13261 atcccgtcag tcggagccaa atctggcaac tcaatactaa aggatttgcc gtctggtgca
    13321 aatgttagtg tcacttgatc agtaatcgtt tctatattat ccagtaccca atcgccggct
    13381 ttaaaactcc agtttccctt ctcaactctc aatgtatctg gaatatactc taattgcttg
    13441 ctttttaaaa catcaaccac cttgacatct ttcatacttt ggccagaacg gttgattgca
    13501 ataatgaacc atgcatcttg tgggttcttt gaatcactcc aaccactttt ttgaatgtct
    13561 cgtttatctg gtgttccaat tccttggaat tgaattttac cagcatggat aatctttcct
    13621 tgtactgtaa tatcaagtgg aatttctttt ttctgctgaa taactctatg atctacacga
    13681 acataaaaat agaatgaccc ttttatatcc gaatgttctt caacatactt tgtataagtc
    13741 aatttgattt tcttttctgg ctctggagtg attgtcgcat tcgcaacaac gttaccctct
    13801 ttgtccgtaa tttggaaatc agaactttgg ttaaatatca gttcagaagg caattcaatt
    13861 tctatcacat ctccttcttt aaccgcattg tttttgatat caaaatcagc attcagacga
    13921 aattgctgcc actgttcgac atttggtggc gttaattcat tcccatcctt atctgtaaag
    13981 tacatttttg taataacatt tgtggtctcg tccccataca catgtgaact cgcccccaaa
    14041 taaccactaa acagaatcat gactggaagt aggatgaaag caagtatttt cttcatcttt
    14101 gtcataacaa actccttaat taaagtaatt tagttaactc ttttaatttc actatctgtg
    14161 ccattttatc actattgtaa cttttcgtca at
//
LOCUS       DAZQCJ010000045        14125 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000045 DAZQCJ010000000
VERSION     DAZQCJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14125
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.046"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            18..1298
                     /gene="aroA"
                     /locus_tag="ACHU66_001221"
     CDS             18..1298
                     /gene="aroA"
                     /locus_tag="ACHU66_001221"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR3389509.1"
                     /translation="MKLRRSVEKLQGVIRVPGDKSISHRSIIFGSLAKGVTRVHDILR
                     GEDVLSTMQVFRDLGVKIEDNGDIVEVHGVGFDGLQAPKNDLDMGNSGTSIRLISGVL
                     AGQDFEATMFGDDSLSKRPMDRVTIPLSQMGVEISGQTERDLPPLTIKGNKNLKPIRY
                     QLPVASAQVKSALIFAALQAEGESVIVEKELTRNHTEDMIVQFGGQLEVNGKEIRIQG
                     GQEFIAQEITVPGDISSAAFWLVAGLIVPGSKIVLENVGINETRTGILDVIKAMGGKM
                     TLSNIDELAKSATITVETSELKATEIAGELIPRLIDELPIITLLATQAHGTTIIRDAE
                     ELKVKETDRIQVVADALNSMGATIEPTEDGMIIHGPTALHGAEINTFGDHRIGMMTAI
                     AALLAKDGEVVLERAEAINTSYPAFFEHLNSLMD"
     gene            1307..1798
                     /locus_tag="ACHU66_001222"
     CDS             1307..1798
                     /locus_tag="ACHU66_001222"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFR3389510.1"
                     /translation="MPIVLLGFMGVGKTTTAHLLNLPVYDMDHIIEERIGMPIADYFS
                     LEGEASFRQLETEVLKELLDLPSNCIVSTGGGVIKSEVNRELLLANREDNVLLTASFE
                     VSYQRIRKDRQSQRPLFLQCSKEEFEALYRERMALYQGLADTVIDTDKLNPEQVARKI
                     LCK"
     gene            1789..2616
                     /gene="pheA"
                     /locus_tag="ACHU66_001223"
     CDS             1789..2616
                     /gene="pheA"
                     /locus_tag="ACHU66_001223"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFR3389511.1"
                     /translation="MQVAYLGPRGSFTHQVAQQAFPTADLQAFETITEVIKAYETGEV
                     TYSVIPVENSIEGSVHETIDYLFHQAEIHAVAEIVQPIAQQLLATSADKAVEVIYSHP
                     QAIAQGKKYIQEHFPQARIEITASTAYAARFVAEHPEQPFAAIAPQAAAVEYGLEVIA
                     QDIQEISENFTRFWILGDKAPSIALEQVAKKVSLALTLPDNLPGALYKAMSVFSWRGI
                     SLTKIESRPLKTALGEYFFILDLDNQSEELLAYSQKELTSLDITYKVLGNYLVYMTS"
     gene            2628..3950
                     /locus_tag="ACHU66_001224"
     CDS             2628..3950
                     /locus_tag="ACHU66_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFR3389512.1"
                     /translation="MNKNNNILTHHEQLRLDYLQKNIHYLNDKESRELDYLLYKKELR
                     ERHGSPRQSLRSFTPTAYEDDFEEDYFEEDDEQEELLPRYPERKSRKTRQAKPQRFQS
                     PRIEPKAKVPKPKKARKKGGFKRFIGFVLLAIALVIVAMGYSFFKGMNSVSEKPVAEV
                     FNGVDTSNGTNILILGTDGRVGESSGETRTDTIMVLNVNNTDNKVKLVSFMRDTLVDI
                     EGYEYKLNTAYTLGEQDNQQGAEEVRKALKNNFDIDIKYYAMVDFATFATTIDTLFPE
                     GVTIDAQFSTINGEVVSSVEVPNDLQLEENAPAYQTIQVGVQQMDGKTLLNYARYRSD
                     DEGDFGRTRRQQEVLSAVMTQAKNPMKLFSGSEAIGKVVAMTPTTVPQSFMWLQGPGL
                     LLDAANGIERVTIPENGDWIDDYDMYGGMALRVDFEKYQQRLAELGLR"
     gene            4017..5375
                     /gene="rlmD"
                     /locus_tag="ACHU66_001225"
     CDS             4017..5375
                     /gene="rlmD"
                     /locus_tag="ACHU66_001225"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR3389513.1"
                     /translation="MLKKNDIVEVEVVDLTHEGQGVAKIDGFVFFVDNALPGEKISMR
                     ILKLKKNIGFGKVEEWQSFSPDRNQDLDLTYLRTGIADLGHLTYPAQLAFKKKQVENN
                     LRKIAGLPEIAVADTLGMDNPLAYRNKAAVPVRRVNGQLETGFFRKNSHDLVPIEDFY
                     IQHKEIDALVLATRDLLRKLDLKPYDEKEQTGLVRNIVVRRGHYSGQLMLVLVTTRPK
                     IFRVETLVERLTSQFPNLVSIVQNINDQNTNAIFGQEFRLLYGSETITDTMLGNEFEI
                     SAPSFYQVNTEMAEKLYQTAIDFAELSADDVVIDAYSGIGTIGLSFAKQVKHVYGVEV
                     VEKAVLDSQKNATRNGIDNVTYVCDSAESAMQKWVAEGVKPTVIFVDPPRKGLTESFI
                     KASTSVKPEKIVYISCNVATMARDIKLYEELGYKLTKVQPTDLFPNTHHIEVVSLLEK
                     RN"
     gene            complement(5456..5709)
                     /locus_tag="ACHU66_001226"
                     /pseudo
     CDS             complement(5456..5709)
                     /locus_tag="ACHU66_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            5935..7047
                     /gene="glf"
                     /locus_tag="ACHU66_001227"
     CDS             5935..7047
                     /gene="glf"
                     /locus_tag="ACHU66_001227"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HFR3389514.1"
                     /translation="MKKYDYLVVGAGLFGAVFAHEAAKKGKKIKVIEKRDHIAGNIYT
                     KEVEGIQVHEYGAHIFHTSEKEIWDYVNQFAEFNSYTNTPVANYKGEIYNLPFNMNTF
                     NKLWGVVTPAEAEAKIAEQRAVLGGKTPENLEEQAISLVGTDIYEKLIKSYTEKQWEK
                     PCTELPAFIIRRLPVRLTYDNNYFNDTYQGIPIGGYTQIVEKMLEHENIDVETNVDFF
                     ANKEDYLATYPKIVFTGMIDEFFDYQLGELEYRSLRFETEVLDMENYQGNAVVNYTDS
                     ETPYTRIIEHKHFEFGTQPKTIITREYSKTWKRGDEPYYPVNNNRNNYLYTVYKKLSE
                     QQENVIFGGRLGHYRYYDMHQVIGAALQCVRNEVGE"
     gene            complement(7146..7691)
                     /locus_tag="ACHU66_001228"
     CDS             complement(7146..7691)
                     /locus_tag="ACHU66_001228"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR3389515.1"
                     /translation="MANVLFLVGSLRDGSFNHQMAKKAEALLTDKAQVSYIDLAKIPL
                     FNQDIETPILPEIAELRAQVDAADAIWIFSPVYNYAIPGIVKNALDWLSRSLDLSNPK
                     GPSILQDKITTVSAVANSGHDHLFKDFQHLLPFIRTQIAGEFTGSTINPEAWGTGVLE
                     LSDETLTKLEQQVADLLAAIQ"
     gene            complement(7754..8203)
                     /locus_tag="ACHU66_001229"
     CDS             complement(7754..8203)
                     /locus_tag="ACHU66_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFR3389516.1"
                     /translation="MRTVEQSLNENKHALHSLVVFRRAANTIAKSELETIKKYGLTVC
                     QFGVMEALYNKGNLRIQDLIDKLLSTSGNMTVVIKNMIRDGYIYKTIDTNDRRASLIA
                     LTPLGRETIEKILPEHYDHVGEIFSVLSSEEQDQLAEILKKFKNQQT"
     gene            8506..9003
                     /locus_tag="ACHU66_001230"
     CDS             8506..9003
                     /locus_tag="ACHU66_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFR3389517.1"
                     /translation="MEKKIFQLLEWMASKTGQLVLGSFILLSVVTFSIFTIWDIAAAP
                     FNNVRSHAVAVATEYADLQTVNDFSIYNGTETYFCVFGVTSQGEEVAVLIPEASSTVY
                     VYPLVQGISQEEAQAIAKKNGASQVERTILGLRDGKPIWEVKSGTAYYLVEFETGNFI
                     KREGL"
     gene            9000..10181
                     /locus_tag="ACHU66_001231"
     CDS             9000..10181
                     /locus_tag="ACHU66_001231"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFR3389518.1"
                     /translation="MSKLSRRVLEMEESVTLAAGARAKALKAEGRDILELTLGEPDFV
                     TPKNIQEAAIKSIEDGKASFYTVASGHPELKDAVNTYFENFYGYSIERNQVVLATGAK
                     FVLYAFFAAVINPGDEVIIPTPYWVSYADQIKMNDGVPVFVTAREEHNFKVTVDQLEA
                     ARTDKTKVFLLNTPSNPTGMIYSREELEAIGNWAVEHDILILADDIYGRLVYNGNGFT
                     PISSISESIRQQTIVVNGVAKAYAMTGWRVGFAVGNPEIIAAMSKIVGQTTSNLTTAA
                     QYAAIEAFTGPQDTVETMRQAFEERLNTIYPLLAEVPGFEVIKPEGAFYLFPNVKKAM
                     EMKGYTDVTEFTTAILEEVGVAMVTGAGFGAPENIRLSYATDMDTLKEAVARLHTFMK
                     K"
     gene            10196..11542
                     /gene="asnS"
                     /locus_tag="ACHU66_001232"
     CDS             10196..11542
                     /gene="asnS"
                     /locus_tag="ACHU66_001232"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR3389519.1"
                     /translation="MSRKLITINQVKDYVGQEVTIGAWVANKSGKGKLAFLQLRDGTA
                     FFQAVALKPNFIEKFGEEAGTEKFDVAKRLSQETSVYVTGIVKEDERSKFGYELDVTD
                     LEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAIQQIRNAIIYATYEFFEKNGF
                     IKFDSPILSGNAAEDSTELFETDYFGQPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVIDRAPQALETLERDVD
                     ALKRYIAEPFKRVAYDDAITLLQEHEADEDTDYEHIEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYEFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRLHP"
     gene            11814..12041
                     /gene="relB"
                     /locus_tag="ACHU66_001233"
     CDS             11814..12041
                     /gene="relB"
                     /locus_tag="ACHU66_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFR3389520.1"
                     /translation="MGIVSLRLNDAEEEIFRSYAIHTGKSLSELFKTALAEQIEDQLD
                     YEIGIKALKKFQENPVTHSIDDVLAELEDGL"
     gene            12031..12300
                     /locus_tag="ACHU66_001234"
     CDS             12031..12300
                     /locus_tag="ACHU66_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR3389521.1"
                     /translation="MAYKLVLSDDALKQLKKMDRHVGMMLAKDLKKRLDGLENPRQFG
                     KALVGDYKGLWRYRVGNYRVICDIIDNKMVILALEIGHRKEIYKK"
     gene            12706..14025
                     /locus_tag="ACHU66_001235"
     CDS             12706..14025
                     /locus_tag="ACHU66_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFR3389522.1"
                     /translation="MKQESLGKEIIRLSLPATVENIFQTLVGFVDTLLIAQLGLVAVT
                     AVGLANTILNVYLAVYMALGVGATALIARSIGAGDRESLTFHVRQALVLSVGVGVLFG
                     LLSLVFGRQMLVLMGADAESLAGAQAFFYWVGGLTIFQALMTILGTILRASGDTVSPM
                     KMSLLTNGFNVVLDYFLIFGIGSWSGLGIVGTALGTVLARLLGTVLLYRKVQQTDLAV
                     DLKQLFHLGSPKEMVDLTLPAAAERLVMRLGQVVYFSLIVGLGTTVYASHMIAGNIES
                     FTYMPAYGLATAAAVLIGQALVKGDILTVRRVAFLSSAYGVAIMFLLGTILFFGAPSF
                     ALLFTKDLEAVHQVVTALRIDAFNQPGLAVSLNMAGALQGLGDTKSPLYSTVIGMWGL
                     RVVGAIVLGQMFGLGIAGVWLSILIDLLLRAIFLTWRFHVKTRKLAE"
BASE COUNT         4075 a         2595 c         3323 g         4132 t
ORIGIN      
        1 tagtttgagg taaggatatg aaattaagaa gaagcgtaga aaaactacag ggtgtgattc
       61 gtgtcccagg tgataaatca atcagtcatc gttctattat ttttggttcc cttgcaaaag
      121 gtgttacccg tgtccatgat attttgcgtg gggaagatgt cttgtcaacc atgcaggtct
      181 ttcgtgattt gggtgtaaaa attgaagaca atggtgatat tgttgaagtc cacggtgttg
      241 gttttgatgg tcttcaagca ccaaaaaatg atttggatat gggaaattct ggaacctcga
      301 ttcgtttgat ttcaggcgtt ttggcaggtc aagactttga ggcgactatg tttggtgatg
      361 attccttgtc caaacgtccc atggaccgag tgactattcc actcagtcaa atgggagtag
      421 agatttctgg acaaactgaa cgtgacctac ctccgttgac catcaaaggg aataagaatt
      481 taaaaccaat tcgctaccaa ctccctgtag cctctgccca agtaaaatca gctctgattt
      541 ttgctgccct gcaggctgag ggagagtcgg tcatcgtaga aaaagagttg actcgaaacc
      601 atacagaaga tatgattgtc cagtttggtg gacagttgga agtcaatggc aaggaaatcc
      661 gcatccaagg tggtcaggag tttattgccc aagagattac agttccagga gatatttcaa
      721 gtgctgcctt ttggttggtt gctggcttaa tcgtaccagg ttcaaaaatt gtccttgaaa
      781 atgtgggaat caatgaaact cggactggta ttctagatgt cattaaagct atgggcggaa
      841 aaatgaccct ttctaacata gatgaacttg caaaatctgc taccattaca gttgaaacgt
      901 cggaattgaa ggctacggag attgcaggcg aattgattcc tcgtttgatt gatgagttgc
      961 cgattattac cttactagcg acccaagcac atggaacaac gattattcgt gatgctgagg
     1021 agttgaaagt caaagaaacg gatcgtattc aggttgttgc agatgctcta aatagtatgg
     1081 gagcaactat tgaaccaaca gaagatggta tgattattca tggaccaact gctttacatg
     1141 gtgctgaaat caatacattt ggtgaccacc gtatcggtat gatgactgcc attgccgcct
     1201 tgttggccaa agatggagaa gttgtattgg aaagagcaga agctatcaat actagctatc
     1261 ctgccttctt tgaacactta aatagtttga tggattagga gaacgtatgc caatcgtttt
     1321 acttggattt atgggagttg gaaaaacaac aacagcacat ttgttaaatc tccccgttta
     1381 cgatatggac catatcattg aagaacggat tggtatgcct attgctgact atttcagtct
     1441 tgaaggagag gcttcgttta gacaactgga gacagaggta ctgaaagaat tgctggattt
     1501 acctagtaat tgtatcgtgt caactggtgg tggggtgatt aaatctgaag taaacaggga
     1561 attgctgttg gcaaataggg aggacaatgt acttcttact gcctcctttg aagtttccta
     1621 tcagagaatt cgtaaggata gacaatcgca aagacctctc tttttacagt gtagtaagga
     1681 agagtttgaa gccctctatc gtgaacgaat ggcactttat caaggtttag ctgatactgt
     1741 aatcgataca gataaactga atccagaaca agtagcaagg aaaattttat gcaagtagcc
     1801 tatctaggac ctcgtggttc ttttacacat caagtggccc agcaggcctt tccgactgcg
     1861 gatttgcagg cttttgagac gattacagag gttatcaagg cctacgaaac gggagaagtt
     1921 acctattccg ttattccagt agagaactcg attgaaggta gtgttcatga aacgattgac
     1981 tatctctttc atcaggcgga gattcatgcg gttgcagaga ttgttcaacc gattgcccaa
     2041 caactgttag ccacgagtgc cgataaggct gtagaggtta tctattctca tccgcaggca
     2101 attgctcagg ggaagaaata tattcaggaa cattttcctc aggctcgtat tgaaataaca
     2161 gccagtacag catatgctgc tcgctttgtg gcagaacacc ctgaacaacc attcgcggca
     2221 attgctccgc aagcggcagc agttgaatat ggactggaag taattgcgca agacatccag
     2281 gaaatcagcg agaattttac gcgcttttgg attttggggg ataaggctcc gtctattgcc
     2341 ctggaacaag tagctaaaaa agtcagtctt gcacttacat tgccagataa cctgccagga
     2401 gccttgtata aggccatgtc tgttttctca tggagaggaa tcagcttaac aaaaatagaa
     2461 agccgacccc tgaaaacggc tttgggtgag tattttttca tactggatct tgacaatcag
     2521 tctgaggaat tattagccta ttctcaaaaa gaattgacta gtttagatat aacctataaa
     2581 gtactaggga attacctggt ctatatgacc agctaggatg aagacagatg aataaaaaca
     2641 ataatatcct aacccatcat gaacaattga ggttagatta tttgcaaaaa aatatccact
     2701 acttaaatga caaagaaagt cgcgagttgg attatctatt atataaaaaa gagttgagag
     2761 aacggcatgg aagtccaagg caatctctac gtagcttcac accaacggct tatgaagatg
     2821 acttcgagga agattatttt gaagaggatg atgagcaaga ggaactgttg cctcgctatc
     2881 ctgaacgtaa atctagaaaa acaagacaag ccaaacctca acggtttcag tctcctcgta
     2941 tcgagcccaa agcgaaagta cctaaaccta aaaaagctcg taaaaagggt ggtttcaagc
     3001 gctttattgg tttcgtattg cttgctatag cgctagtgat tgtagcgatg ggctatagtt
     3061 ttttcaaagg tatgaatagc gtgagtgaga agccagtagc tgaggtcttt aacggcgtag
     3121 atacatcgaa tgggacaaat attttaattt tgggaacaga cggtcgtgtt ggcgaaagct
     3181 caggtgaaac tcgaacggat accatcatgg ttctaaatgt gaataacacg gataacaaag
     3241 tcaaacttgt tagctttatg cgggatacct tggtggatat tgagggatat gaatataaac
     3301 tcaatactgc ctatactttg ggagaacagg ataatcagca aggggctgaa gaagttcgga
     3361 aggcgttgaa gaataatttt gacattgata tcaagtacta tgcgatggtg gattttgcaa
     3421 cctttgcgac aacgattgat acacttttcc ctgagggcgt aacgattgat gcacagtttt
     3481 caactattaa tggtgaagtc gttagctcag tagaagtgcc gaatgattta cagctggaag
     3541 aaaatgctcc tgcttatcaa acgattcagg tcggtgtaca gcagatggat ggtaagacct
     3601 tgcttaacta cgcgcgctat cgttcggatg atgaaggtga ttttggtcgt acgagacgtc
     3661 aacaggaagt gttatctgct gttatgacac aggctaaaaa tccaatgaaa ctattctctg
     3721 gctccgaagc tataggtaag gttgtggcca tgactccgac aacagtgcca caaagcttta
     3781 tgtggctaca gggaccaggc cttctattgg atgcggcaaa tggtatcgaa cgtgttacaa
     3841 tcccagaaaa tggagactgg attgacgact atgatatgta tggtggtatg gctcttcgtg
     3901 ttgattttga aaaataccag cagcgcttgg ctgagttagg cttgcgttaa gaaatatggt
     3961 atactagaag tgacttcggt cactttttta gtggtccaaa aatagaaagt agaattatgt
     4021 taaagaaaaa cgatattgtc gaagtagaag ttgtagattt gacacatgag gggcagggtg
     4081 tagcaaaaat tgatggtttt gtcttctttg tagacaatgc tcttccaggt gagaaaattt
     4141 ccatgcgcat cttaaaactt aagaaaaata ttggttttgg taaggtggaa gaatggcaga
     4201 gcttctcacc agaccgtaat caggacttgg atttgaccta tctaagaaca ggtattgctg
     4261 acttggggca tctcacttat ccagcccaac tagcttttaa gaaaaaacaa gttgagaata
     4321 atctacgtaa gattgcaggt ctgccagaaa tagcagtagc agatactttg ggaatggaca
     4381 atcctcttgc ttatcgcaat aaggcagctg ttcctgttcg tcgtgtgaat gggcagttag
     4441 aaacaggatt tttccgaaaa aattcacatg atttagttcc gattgaggat ttttacattc
     4501 aacataagga aattgatgcc ttggttctag cgacacgtga cctgttgaga aaattagact
     4561 tgaagcctta cgatgagaaa gaacagacag gacttgtgag aaatattgtt gttcgtcgtg
     4621 gtcattattc aggtcagttg atgctagttt tagttacgac tcgtccgaag attttccgtg
     4681 ttgaaaccct tgttgagcgt ttgacaagcc aatttccaaa tcttgtttct atcgttcaga
     4741 atattaatga ccagaatacc aatgctatat tcggtcaaga attccgtctt ttgtatggca
     4801 gtgaaactat tacagatacc atgttgggca atgaatttga aatttctgca ccgtctttct
     4861 atcaagtcaa cacagaaatg gctgaaaaac tctatcagac cgccattgac tttgcagaat
     4921 tgtcggcaga tgatgtcgtg attgatgcct attcaggtat tgggacaatt ggtctctcct
     4981 ttgcaaaaca agttaagcat gtttacggtg tagaagtcgt tgaaaaggct gtccttgata
     5041 gtcagaagaa tgctactcga aatgggattg ataatgttac ctatgtctgt gatagcgctg
     5101 aatccgctat gcaaaaatgg gtggcagagg gagtcaagcc aactgtgatt tttgtcgacc
     5161 caccacgaaa aggtttgaca gaaagtttta tcaaagcaag tacttctgta aaaccagaga
     5221 aaatcgtcta tatctcctgt aatgtagcaa ccatggcgcg tgatattaaa ctttatgagg
     5281 aattggggta caaattgacg aaggtacagc cgactgattt atttccaaat acgcatcaca
     5341 tagaagttgt gagtctatta gaaaaacgca attagattgt ccagcttgct ctcttgtttt
     5401 ctggcaagct gcttcaaagg tccgcaggac ctttgaactc ccaaatacgc atcatgttga
     5461 cagttgccac acctacacca aaatcaaatg ccaacaagcg ttgagtcggg taatagcgta
     5521 agtagcgcaa ggtcatgata agctgctctt ccatacttag acggcgtggg cgtcctcctt
     5581 ttcggtgttg ctcttgataa gcgtcagtga gacaatcaag catcagatga aacgtcgctt
     5641 ttttacacct atcaacaatt tgaaattctc tgagtttaat tttaagactt tttcgtatgt
     5701 tgtttccata cctttagtat accgcctttg agttaccgaa caagtctatt gctaaacttg
     5761 atgaaggttg tattgtctgt tataatattg gatattgcaa gctttaagtg aaaggtttta
     5821 gggaagagat tatcacaact cattctttat tagttatgag acgatggtat taatgatagt
     5881 ctagtaaaag atttggagaa atgacttcct tagaaagtta aatgtaaaaa caatatgaaa
     5941 aaatacgatt atctagttgt cggagcgggt ctgttcggag cggtctttgc tcatgaggca
     6001 gctaaaaagg gtaaaaaaat aaaggttatc gaaaaacgag accacattgc gggtaacatc
     6061 tatacaaaag aagtagaagg tattcaagtc catgaatatg gcgcacatat tttccatact
     6121 tctgaaaaag aaatttggga ttatgtgaat cagtttgcgg agttcaacag ctatacaaac
     6181 acaccagtcg ccaactacaa aggtgagatt tacaatcttc ccttcaacat gaatactttc
     6241 aataaactgt ggggcgtggt gacacctgct gaagcagaag cgaagattgc agagcaacga
     6301 gctgtgttgg gtggtaaaac gcctgaaaac ttggaagagc aagccatctc acttgttggt
     6361 acagacatct atgaaaaatt gatcaagtcc tacacagaaa aacaatggga aaaaccatgt
     6421 acggagttac cagccttcat catccgtcgt ttgcccgtgc gtttgactta tgataataac
     6481 tactttaacg atacttatca aggtattcca atcggcggtt acacgcagat tgtagaaaaa
     6541 atgttggaac atgaaaatat cgatgtggag acaaatgttg atttctttgc caataaggaa
     6601 gactatttag ctacctatcc aaagattgtc tttacaggaa tgattgacga gttctttgat
     6661 taccaacttg gtgaattgga gtaccgtagc cttcgatttg agacagaagt gttggatatg
     6721 gaaaactatc aagggaatgc ggttgtcaac tatacagata gcgagacacc atatactcga
     6781 atcatcgaac acaaacactt cgaatttggt acacaaccga aaaccattat tacacgtgag
     6841 tattcgaaga cctggaaacg aggcgatgag ccatactatc cagtcaacaa taatcgcaat
     6901 aactatctat acacagtata taagaaattg tcagaacaac aagagaatgt catattcggt
     6961 gggcgtcttg gtcattatcg ttactacgat atgcaccaag tcatcggagc tgccttacag
     7021 tgtgtgagaa atgaagttgg ggaatgattt tgagaagtca tcaaagtagt aattattctt
     7081 ggcagaagtc ggaatgctct taaaatgtta atcaaaaaaa ccagaaattc tggttttttt
     7141 gattattatt gaattgctgc caacaggtca gctacttgtt gttcgagttt tgttagagtt
     7201 tcgtcagata actccaatac acctgttccc catgcttctg gattgatggt tgaacctgta
     7261 aattcaccag caatctgagt gcgaataaat ggaagaaggt gttggaagtc tttgaatagg
     7321 tggtcgtgac cactgtttgc gactgcagag acagttgtga ttttatcttg taggatagat
     7381 ggtccttttg gattggataa atcgagcgaa cgactgagcc aatcgagagc attttttacg
     7441 atgcctggga ttgcatagtt atatactggt gagaaaatcc agatagcatc cgcagcgtct
     7501 acttgtgcac gtagctctgc aatttcagga aggataggtg tctcgatgtc ttggttgaat
     7561 aggggaattt ttgcaagatc aatgtaagat acttgagcct tatcagtcaa gagtgcttca
     7621 gctttttttg ccatttgatg gttaaaagaa ccgtcacgta gtgaaccaac taaaaacaat
     7681 acgtttgcca tgatggcctc cttttcataa aaacataaat ttagtatatc gtatttgtct
     7741 tttgttgaca atcctaggtc tgctgattct tgaatttttt aagtatctcg gctaattggt
     7801 cttgttcttc tgaactaagg acagaaaaaa tctctccaac gtgatcataa tgctctggaa
     7861 ggattttttc aatggtttcg cgtccaagtg gagtgagagc aatcaaggaa gcacgacggt
     7921 catttgtatc gatagtcttg tagatataac catctcgaat catgttcttt ataacgacag
     7981 tcatgttgcc agaagtgctt aataatttat caattaagtc ttgaatccgc agatttcctt
     8041 tgttgtagag tgcctccatc actccaaact gacaaactgt cagtccgtat tttttgattg
     8101 tttctaattc tgattttgcg atagtattag ctgctctgcg aaagacgact agactgtgaa
     8161 gtgcatgctt gttctcgttt aaagattgtt caacagttct catgagaata ttttagcaat
     8221 aaatcgttcg gttatcaagc aaaaaaagtt aaatcctata ttcataggta aagtactttt
     8281 atggaaaaga tagtttttag ttaatcaagt tcgttttttg aggtaatact aactgtactg
     8341 gaaaaaaacg agcaatgagc atttgagaaa tagtcttaga tggaagtgca aaactgtgaa
     8401 tgtaggtttt tatatccttt tctttgtgtg aaggaaaagg aaattttatt tacaatcacg
     8461 gaaagaatgg aaatatgctt taatttgtag tataatggga ctactatgga aaaaaagatt
     8521 tttcaactgt tggagtggat ggcttccaag actggtcagt tagtgttggg ctcttttatc
     8581 cttctcagtg ttgtgacttt ttcaattttt actatttggg atattgctgc tgcccctttc
     8641 aacaatgtgc gtagtcatgc agttgcagta gctacagaat atgctgattt gcaaactgtc
     8701 aatgattttt ctatctacaa tggtactgaa acctattttt gtgtatttgg tgtcaccagt
     8761 cagggagaag aggttgcagt tttgattcca gaggcttcta gcacagtata tgtctatcct
     8821 ttagttcagg gaatttcgca agaagaagcg caagctattg ccaagaaaaa cggtgcaagc
     8881 caagtagagc gaactattct aggtttaagg gatggcaaac ctatttggga agtcaagtca
     8941 ggtactgcct actatttagt tgaatttgaa acagggaact ttatcaaacg ggagggctta
     9001 tgagcaagtt atcaagacga gtcttagaga tggaagaaag tgtaactctg gcagcaggtg
     9061 cgcgtgcaaa agccttgaag gcggaaggac gtgatatttt agagctgacc ttgggtgagc
     9121 cagacttcgt cacaccgaaa aatattcaag aagctgctat taagtctatc gaagatggta
     9181 aggcgagttt ttatacggta gcttcagggc atcctgagtt gaaggacgca gtcaatacct
     9241 actttgagaa tttctatggc tactctatcg aacgcaatca agtcgttctt gcgacaggtg
     9301 ctaagtttgt tctttatgct ttctttgcgg cggtcatcaa tcctggtgat gaggtgatta
     9361 ttccgacacc ttactgggtt tcttatgcag atcaaatcaa gatgaatgat ggggtgcctg
     9421 tctttgtcac tgcaagggaa gagcataatt tcaaggtaac ggttgaccag ctggaagcag
     9481 ctcggacaga taagaccaaa gtattcctac tcaatactcc ttctaatccg actgggatga
     9541 tttacagtcg tgaggaattg gaagcaattg gaaactgggc tgtagagcac gatattctca
     9601 tcttggcaga cgatatttat ggtcgtttgg tctataatgg aaatggcttc acaccgattt
     9661 caagcatttc agaaagcatt cgtcagcaga ctattgttgt caatggggtg gcaaaggcct
     9721 atgctatgac aggttggcgg gttggttttg ccgttggtaa tcctgaaatc attgcggcca
     9781 tgagtaagat tgtcggacaa acgacttcga atttaacgac tgctgctcag tatgcggcca
     9841 ttgaagcctt tacaggtccg caagatacag tagaaaccat gcgtcaggcc tttgaagagc
     9901 gactcaatac catttatccc ttgctggcag aagtaccagg ttttgaagtg attaagccag
     9961 aaggggcctt ctatctcttc ccgaatgtta aaaaagctat ggaaatgaaa ggctacacag
    10021 atgtaactga atttaccaca gccattttgg aagaagtggg agttgcaatg gtgacaggtg
    10081 ctggttttgg agctcctgag aatatccgcc tcagctatgc gacagatatg gacacgctca
    10141 aagaagcagt ggctcgacta catacattta tgaaaaaata aaaagaggaa aaataatgtc
    10201 tagaaaattg attaccatta accaagtcaa agattatgtc ggtcaagaag tgactattgg
    10261 tgcctgggtt gccaacaaat caggcaaggg caagttagcc ttcctgcaat tgcgtgacgg
    10321 aacagccttc ttccaagcag ttgccttaaa accaaacttc atcgaaaaat tcggtgaaga
    10381 agctgggact gagaagttcg atgtagcaaa acgcctcagc caagaaacgt ctgtctatgt
    10441 gacaggaatt gtcaaggaag atgagcgttc taagtttggt tatgagttgg atgtgacaga
    10501 ccttgaagta atcggtgagt cacaagatta cccaatcaca ccaaaagaac acggtacgga
    10561 cttccttatg gacaaccgtc acctctggtt gcgttctcgt aaacaggtcg ctatccagca
    10621 aatccgtaac gccatcatct atgcgactta tgaattcttt gaaaagaatg gctttatcaa
    10681 gtttgatagc ccaatcttgt ctggaaacgc tgcagaagat tcgacagagt tgttcgaaac
    10741 tgactacttt ggtcaacctg cctacctcag ccaatctggt cagctgtacc tggaagctgg
    10801 tgccatggcc cttggtcgtg ttttcgactt cggacctgtt ttccgtgcgg aaaaatcaaa
    10861 aactcgccgt cacttgacgg agttctggat gatggatgcc gagtattcat tcctcagcca
    10921 tgaagagtca cttgatttgc aagaagctta tgtcaaagcc ttgattcaag gtgttatcga
    10981 ccgtgctcca caagccttgg aaactcttga gcgtgatgtg gatgctctca aacgctacat
    11041 tgcggagcca ttcaaacgtg tggcttatga tgatgccatt acccttcttc aagagcatga
    11101 agctgatgaa gatacagact acgaacacat cgagcatggt gatgactttg gatcgccaca
    11161 tgaaacttgg atttcaaact actttggtgt accgactttc gttgttaact atccagcaag
    11221 cttcaaggcc ttctacatga agccagttcc aggtaatcca gagcgcgtgc tttgtgcgga
    11281 cctcttggca ccagaaggct acggtgaaat catcggtggt tctatgcgtg aggacaatta
    11341 cgatgccttg gtagccaaga tggacgagct cggcatggat aagtcagaat acgaattcta
    11401 ccttgaccta cgtaagtacg gttcagtacc acacggtggt ttcggtatcg gtatcgagcg
    11461 tatggtgact tttgttgcag gtacaaaaca catccgtgaa gccattccgt tcccacgtat
    11521 gttgcaccgc ttgcatccgt aatacagtcg ttcagttttt cctttattac gtcgacgtat
    11581 gaggaaactc cgtttcctta tttccaagtt caaataatct catcattatt tgaactcgtt
    11641 ttgccgtacc ttagtacagt ctgcagctcg ttgcctagta ctaaaagtaa actgaaagac
    11701 tgcgaaataa atattcactg acagactaca aacgtggtct gtttttctgg tgtcaattga
    11761 cattttgaac gaattgtttt acaatgtatt acaagaagaa agtaggtgga atgatgggaa
    11821 ttgtatcctt gcgattaaac gatgcagaag aagaaatttt cagaagctat gctattcata
    11881 caggaaaaag cctgtctgag ctattcaaaa ctgcacttgc tgaacagata gaagaccagt
    11941 tggactatga aattggtatc aaggctttga agaaattcca agaaaatcct gttacacact
    12001 cgattgatga tgttcttgcg gagttagaag atggcttata agcttgttct cagcgatgat
    12061 gctttgaagc agttgaaaaa aatggacagg catgtgggga tgatgcttgc taaggatttg
    12121 aaaaaacgtt tggacggttt ggagaatcca agacaatttg ggaaggcctt ggtaggagac
    12181 tataaggggc tgtggcggta tagagtgggg aattaccgtg taatttgtga tattattgat
    12241 aacaaaatgg tcatcctggc ccttgaaatc gggcaccgga aagaaatcta taaaaaatga
    12301 agttctaggt aactaggctc ggtatcggtg tacttctcag agttcgtgtc aacatctcag
    12361 cgcagtggtt gattggcttt aacagtccag tggagtgaag cagttctggg aactgtttca
    12421 gcccgagcct tgaagcagaa aagctaggta aaaaggttgg agataaggtt tgcgaagcaa
    12481 atctcagcaa ttcgtgtttt ccactccaaa tctgacctat acgactgatg cgaactttgt
    12541 tcgccttatc tctaacctcc aaaggttccc tgaaactttg gagctatgcg ggggtggaga
    12601 gatgaactcc tttttgactt atcgagttct tccccactcc ctgttttctt tctttttcac
    12661 cgagttttat ttataattat tctaaataaa atcgtggaga ggaaaatgaa gcaagaaagt
    12721 ttaggaaaag aaattattcg tttgtccttg cctgctacgg tggaaaatat ctttcagacc
    12781 ttagttggtt ttgtggatac cctcttgatt gcacagctgg ggctggtagc ggtgacggca
    12841 gttggtcttg ccaatacgat tttgaacgtc tatttggcgg tctatatggc tttgggagtt
    12901 ggggcaacag ctttgattgc tcggtctatt ggcgcaggag atagggaatc tcttaccttt
    12961 catgtccgtc aagccttagt gctttcagta ggtgtaggtg tgctatttgg tttactatcc
    13021 cttgttttcg ggcggcagat gctggtctta atgggagcag atgcggagag tttggctggt
    13081 gcccaggcat ttttttactg ggttggtggt ttgacgattt ttcaggcctt gatgactatt
    13141 ttgggaacaa tcctgcgggc atctggtgat acagtatccc ctatgaaaat gagcttactg
    13201 accaatggtt ttaatgtggt cttggactat ttcttgattt ttggtattgg ctcttggtca
    13261 ggtttgggga ttgtagggac agccttggga accgtcttag ctcgtttgct tggtactgtc
    13321 ttgctttacc gaaaggtaca gcagactgac ttggcagttg atctcaaaca attgttccac
    13381 ttgggaagtc ctaaggaaat ggtggatttg accttacctg cagcagccga acgcttggtt
    13441 atgcggttgg gacaggtggt ctatttcagt ttgattgtgg ggctggggac gactgtctat
    13501 gcttctcaca tgattgcagg caatatcgag agttttacct atatgccagc ctatggtctt
    13561 gcgacagcgg cggcggtttt gatcggtcaa gccttggtaa agggggatat cctcacagtt
    13621 agacgtgtcg catttctcag ctctgcctat ggtgttgcca tcatgttcct gctcggtacc
    13681 atcttatttt tcggagctcc aagttttgct ctgctattta ccaaagacct agaagcagtc
    13741 catcaagtcg tcacagcctt acggatcgat gctttcaacc agccaggcct tgcggtttct
    13801 ttgaacatgg ctggcgctct gcagggcttg ggggatacca agtcgccact ctactccaca
    13861 gtgataggga tgtgggggct gcgcgtcgta ggtgctatcg tcttaggtca gatgttcggt
    13921 ttaggaattg ccggcgtctg gctgtcgatt ttgattgacc tgctcttgcg agcgattttt
    13981 ctgacttgga ggtttcatgt aaaaacaaga aaactggctg aataggctag tttttgggta
    14041 taagcaggta gttttagaag tagattatgg attgccttat ttcatgttgc tgatactatc
    14101 tcaattcctt ttttataaaa gaaaa
//
LOCUS       DAZQCJ010000046        14078 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000046 DAZQCJ010000000
VERSION     DAZQCJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14078
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.047"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            202..537
                     /locus_tag="ACHU66_001236"
     CDS             202..537
                     /locus_tag="ACHU66_001236"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFR3389523.1"
                     /translation="MIEGYQEKQFIVASRTEQTDYGNVRFVRDIIGEIMELRSREGGD
                     IWLFGGASLVQDCLEAGVIDHLIIGIIPTILGDGIPLFASLLEEKKLLLVESTVTDGI
                     AMLRYDIRR"
     gene            complement(580..1563)
                     /locus_tag="ACHU66_001237"
     CDS             complement(580..1563)
                     /locus_tag="ACHU66_001237"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFR3389524.1"
                     /translation="MTATKQHKKVILVGDGAVGSAYAYALVNQGIGQELGIIDINKDR
                     TQGDAEDLSHALAFTFPKKIYSAEYSDAHDADLVVLTAGLPQKPGETRLELVEKNLRI
                     NQQIVTEIVNSGFNGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQAL
                     AEKIGIDARSVHAYIMGEHGDSEFAVWSHANVAGVKLYDWLQDNRDIDEQGLVDLFVS
                     VRDAAYSIINKKGATYYGIGVALARITKAIFDDENAVLPLSVYQAGQYEGVEDVFIGQ
                     PAIIGAHGIVRPVNIPLSDAELQKMQASAKQLKDIIDDAFANPEIAAGVKN"
     gene            1752..4196
                     /gene="gyrA"
                     /locus_tag="ACHU66_001238"
     CDS             1752..4196
                     /gene="gyrA"
                     /locus_tag="ACHU66_001238"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFR3389525.1"
                     /translation="MQDKNLVTVNLTNEMKSSFIDYAMSVIVSRALPDVRDGLKPVHR
                     RILYGMNELGITPDKPHKKSARITGDVMGKYHPHGDSAIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVNFADNYDASEREPEVLPAR
                     FPNLLVNGTTGIAVGMATNIPPHNLGETIEAVKLVMDNPEVTTREIMEVLPGPDFPTG
                     ALVMGKSGIHRAYETGKGSIVLRSRTEIEEYGNGRERIVVTEFPYMVNKSKVQEHIVK
                     LVQEKRIEGITAVRDESNREGVRFVIEVRRDASANVILNNLFKQTQLQTNFSFNMLAI
                     QNGVPKILSVRQILESYIEHQKEVVTRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRNSETDAIAQAELMEKFDLSVRQSQAILDMRLRRLTGLERDKIQSEYDELVALIAD
                     LADILAKPERVVQIIKDELEEVKRKYADPRRTELMVGEVLSLEDEDLIEETDVLITLS
                     NQGYIKRLAQDEFQAQKRGGRGVQGTGVKDDDFVRELVSTSTHDRLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLEENEAIQTIINVTKDQEADSYLFFATRQGVVKRTS
                     VSEFANIRQSGLKALNLKEDDELINVFLTNGQADIIMGTKFGYSVRFTETDVRNMGRT
                     ATGVRGINLREGDQLVGATMISDDQEVLVLTEKGFGKRTPASEYPTKGRGGKGIKTLK
                     VADKNGSLAGLTTVSGDEDIMVITDTGVIIRTSVANISQTGRSTMGVKVMRLNDEAKI
                     MTFALVDAAEIKEEKE"
     gene            4203..4952
                     /locus_tag="ACHU66_001239"
     CDS             4203..4952
                     /locus_tag="ACHU66_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFR3389526.1"
                     /translation="MSKREKKKKRKGSFWRNFLTVVLILISLALIFNTSIRNFIIGWN
                     TNKYQISNVTTEDIEKNKQAETTFDFEQVQSISTEAILAAQWDAQRLPVIGGIAVPEL
                     GINLPIFKGVFNTSLMYGAGTMKENQEMGKGNYALASHHIFGVTGAADVLFSPLDRAK
                     NGMKIYITDKTNVYTYVIDSVEIVSPESVYVIDDVEGRTEVTLVTCTDYYATQRIVVK
                     GVLESTTPYNETAKDILDSFNKSYNQYDYGQ"
     gene            5066..5746
                     /gene="radC"
                     /locus_tag="ACHU66_001240"
     CDS             5066..5746
                     /gene="radC"
                     /locus_tag="ACHU66_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR3389527.1"
                     /translation="MYQIEFKEEAILPRERLVEVGAERLSNQELLAIFIRTGTKKEPV
                     SILSNKLLNRLESLAALRELSIEELQSLTGIGRVKAIEIKAMIELGKRINQSELLLNE
                     RILGSEKLGRKMIHEIGHKKQEHLVALYLNTQNQIISQKTIFIGSVNRSIAEPREILH
                     YAVKCMATSIIIVHNHPSGSVQPSRNDLLFTENLKESCEKLGLVLLDHLIVGNKDYYS
                     FREESELF"
     gene            complement(5743..6435)
                     /locus_tag="ACHU66_001241"
     CDS             complement(5743..6435)
                     /locus_tag="ACHU66_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFR3389528.1"
                     /translation="MKKPVIGISGNEYKTGDHTEPLLSYTQTCLVQAIEDAGGLPLIL
                     PVTEPDLAKYYIHLIDKLILTGGQNVQPSYYREERTIDSDNYLPKRDEFELAIIRAAQ
                     ENQKPIFGICRGLQLYNVAQGGSLHQSISEHWQDIDGQEVSQTIQLTQNSPLYDIYES
                     DPSVNSFHRQAIKDLAPDLEIIALSDNQQIIEAVHSAYPTKFLGVQWHPELLYGKREI
                     EKELFHYIVNKL"
     gene            complement(6439..7077)
                     /locus_tag="ACHU66_001242"
     CDS             complement(6439..7077)
                     /locus_tag="ACHU66_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFR3389529.1"
                     /translation="MKNDKKSDIPRATAKRLSLYYRIFKRFYAGNIEKASSKEIAEAI
                     GIDSATVRRDFSYFGELGRRGFGYNVKELMDFFADILNDTSITNVMLVGVGNMGRALL
                     HYRFHERNKMKIVMAFEADDNPAVGTTDENIPIHAISEIKERISEANSQTAILTVPSV
                     KAQEVTDILVEAGVKGILSFSPVNLSVPKDVVVQYVDLTSELQTLLYFMRKG"
     gene            complement(7212..7415)
                     /locus_tag="ACHU66_001243"
     CDS             complement(7212..7415)
                     /locus_tag="ACHU66_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HFR3389530.1"
                     /translation="MIEIHCLDAYKQERIQTFENKDATILAFSGCLTLPDYYPVTSIT
                     QDGQALDYKGTIGDLYRYLQTLD"
     gene            complement(7417..7761)
                     /locus_tag="ACHU66_001244"
     CDS             complement(7417..7761)
                     /locus_tag="ACHU66_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFR3389531.1"
                     /translation="MAFQQSVTLKDCNFTYHISPAIKKYTLRDNTFEQTKAGHYQLTR
                     LLEEIPNSNQGFLLKIIINQDLTGFKINITDKSGLHLVNIFKPESNKVIQEKFYFLMD
                     SLIDRDIFTKES"
     gene            complement(7763..8878)
                     /locus_tag="ACHU66_001245"
     CDS             complement(7763..8878)
                     /locus_tag="ACHU66_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFR3389532.1"
                     /translation="MIYLDNAATTALSPTALQRMMEVAQENYGNPSSIHQSGRKANQR
                     LRQSRQEIAQILSASPEQIIFTSGGSEADTLAIQGYALAHQSKGKHLITTAIEHHAVL
                     HTMQYLEERFGFEVTYIQPVDQVITAQQIQDALRPDTILVSVMFANNETGQLLPIKEI
                     GELLADHQAVFHVDAVQAIGKIAISPSNYGIDLLAASAHKFHGPKGTGFLYSKVHKFD
                     SLIHGGKQEQQHRAGTENLPAIAAMATALTEQYESLDTHHQHVSELRQHIIEGLSGLD
                     YYLNQAGPHLPHVLNIGFPGKLNEQLLMQLDLAGIAVSSGSACTAGVVQNSHVLEAMY
                     GKDSHRLKESIRISLSEINTKEEIDTLIIELKKILGG"
     gene            complement(8889..9866)
                     /locus_tag="ACHU66_001246"
     CDS             complement(8889..9866)
                     /locus_tag="ACHU66_001246"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR3389533.1"
                     /translation="MVQPLGEHNIKLFSLNSNRDIAEKIADSAGIPLGKLSSRQFSDG
                     EIQINIEESVRGVDVYIIQSTSYPVNNHLWELLIMIDACKRASANTITAVIPYFGYAR
                     QDRTASPREPITAKLVANMLVKAGVDRVLTLDLHASQIQGFFDIPVDNLLTEPLFAAY
                     YMEKGLCGEDVVIVSPKNSGIKRARNIAEFLNAPIAIIDYAQDDSERSEGYIIGDVAG
                     KKAILVDDILNTGRTFSQASKIVQEGGATEIYAVASHGLFAGTATQLLDEAPIKEILV
                     TDSVASKEQHPKNIVFLTASDLIADAIHRIQEHQPLSPLFKFTSPEKEN"
     gene            complement(10011..10577)
                     /locus_tag="ACHU66_001247"
     CDS             complement(10011..10577)
                     /locus_tag="ACHU66_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFR3389534.1"
                     /translation="MKNHLEIEYKTLLTKSEYKRLLTDFSDVSPILQTNHYIDTPDLD
                     MKNHRFSLRIRTFEDSAELTLKIPQEVGNQEYNQVLDIQTAHDLLENFQLPAGQISDI
                     ISATEIPLDKLAVWGILTTKRYEKQTSIGLMALDENDYVGQKDYELEVEVTDADAGKI
                     LFDEFLKKKSIQFKYASSKVARTAAHMK"
     gene            10664..11335
                     /locus_tag="ACHU66_001248"
     CDS             10664..11335
                     /locus_tag="ACHU66_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFR3389535.1"
                     /translation="MDFNWEEFLDPYIQTVGELKIKLRGIRKQYRKANRHSPIEFVTG
                     RVKPIESIKEKMALRHIKLENLAQDMQDIAGLRIMVQFVDDIEEVLAILRKRKDMQIV
                     HERDYINNMKASGYRSYHVVIEYPVDTINGNETVLAEIQIRTLSMNFWATIEHSLNYK
                     YKGNFPEEIKKRLEVTAKIAYELDEEMRKIRNDIQEAQALFDPAYRKLNDGVGNSDDT
                     DEEYR"
     gene            11319..12137
                     /locus_tag="ACHU66_001249"
     CDS             11319..12137
                     /locus_tag="ACHU66_001249"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFR3389536.1"
                     /translation="MKNTGRKKIALLASRNPKSEAVSKELWTKLKEANFILTPKNPDI
                     VISIGGDGMLLSAFHKYEKLIDRVRFVGIHTGHLGFYTDYRDFEVDKLIENLKLDTGA
                     RVSYPILNVKVKMTDGRIVEARALNEATVKRLSKTMVADIIINNVPFERFRGDGISVS
                     TPTGSTAYNKSLGGAVLHPTIEALQIAEVASLNNRVYRTLGSSVVVPKKDKIVIEPKH
                     SDRYSIAVDNKTFVYDSIESIEYQIDNSKIHFVATPSHTSFWNRVKDAFIGEVE"
     gene            12137..13030
                     /locus_tag="ACHU66_001250"
     CDS             12137..13030
                     /locus_tag="ACHU66_001250"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFR3389537.1"
                     /translation="MRFEFIADQHTKIKTFLKKHGVSKGLLAKIKYTGGNIWVNDIEQ
                     NATYLLDIGDRVTIDIPAEEDLTGSLKPIFFPLDIVYEDDHFLSINKPVGYASIPSAL
                     HSSTIANFVKGYLIEQNYENKQVHIVTRLDRDTSGVMLFAKHGYAHARLDKQLQAKLI
                     HKRYYALVKGNGQLEETGDIIAPIGRPEDSIITRCVTKNGKYAHTSYRVVQSWGDIHL
                     VDIQLHTGRTHQIRVHFSHIGFPLLGDDMYGGSLECGIERQALHCHNLAFDNPFSAER
                     IDLEAPLPEDFQAVINQLISK"
     gene            13048..14022
                     /gene="pta"
                     /locus_tag="ACHU66_001251"
     CDS             13048..14022
                     /gene="pta"
                     /locus_tag="ACHU66_001251"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR3389538.1"
                     /translation="MKIFEQLATKLEGKKVRIVLPEGEEPRILQATKRLVNESDVVPV
                     LLGNPDRIKIYLDIEGITEGFEIIDPAHYDRYDEMVAALVERRKGKVSEEQAHELLKD
                     VNYFGVMLVYMGVVEGMVSGAIHSTASTVRPALQIIKTEPGVSKTSGAFLMVKGEERY
                     IFGDCAINIDPDAQTLAEIAINSARTAKMFGIDPKVAMLSYSTKGSGAGPKVDKVVEA
                     TKIAQKLRPELDLDGELQFDAAFVPTTGKLKAPGSSVAGQATVFIFPGISAGNIGYKI
                     AERMGGFAAVGPVLQGLNQPVNDLSRGCNPDDVYNLTLITAVQALEHR"
BASE COUNT         4127 a         2490 c         3173 g         4288 t
ORIGIN      
        1 cttgccagtt tttgatgcga cgtctagcgt caaaagagtt ctcgacttaa caagacacct
       61 ctactttatc ataaagaaaa agtagtgagt actttctccc gtcggagatt tcctcactac
      121 taatcttagt atgtttttga ccagtcgagt tctttcccga tcccgatggg gagaaaatca
      181 ttagaagatt gtccgttgga aatgattgaa ggatatcagg aaaagcaatt tatcgttgcg
      241 agtcgtaccg agcagacgga ctatggtaat gtgcgtttcg taagggacat catcggggag
      301 attatggaac ttcgttctag agaaggtgga gatatttggt tgtttggtgg agcaagtctg
      361 gttcaggact gtttagaggc gggagtgatt gatcacctta ttattgggat tattcctact
      421 attttgggtg acggtattcc acttttcgcc tcacttttgg aagaaaagaa attgctcttg
      481 gtagagtcca ctgtcacaga tggaattgct atgttacgtt atgatattag aaggtaaaaa
      541 aggcgagcta ttgctcacct tttttactat tttatataat tagtttttaa caccagctgc
      601 aatttctggg tttgcgaaag catcatcgat gatgtctttt aattgtttag cagatgcttg
      661 cattttttgc aattcagcgt cgctcaatgg gatattgact ggacgaacga taccgtgggc
      721 accgatgata gcaggttgac cgatgaagac atcttcaaca ccttcgtatt ggccagcttg
      781 gtaaactgac aatggaagta ctgcattttc atcatcaaag attgctttag tgatacgtgc
      841 aagtgcaaca ccgataccgt agtaagttgc gcctttttta ttgatgattg agtaggctgc
      901 atcacgaaca gatacaaaca agtcaacaag accttgctca tcaatatcgc ggttatcttg
      961 caaccagtcg tacaatttca caccggcaac gttggcatgt gaccaaactg caaattctga
     1021 gtcaccgtgc tcacccatga tgtaagcgtg aactgaacgt gcgtcgatac cgattttctc
     1081 agccaatgct tgacggaaac gagctgagtc aagagaagta ccagaaccga taacacgctc
     1141 tttagggaaa ccagagaatt tccaagttga gtaagtcaat acgtcaacag ggttagcagc
     1201 aacaaggaag ataccgttga aaccagagtt aacgatttct gtaacgattt gttggttgat
     1261 acgaaggttt ttctctacca attcaaggcg agtttcacct ggtttttgtg gcaaaccagc
     1321 tgtcaataca accaagtctg cgtcatgagc atctgaatac tcagcagagt agattttttt
     1381 agggaatgtg aaggccaatg cgtggctcaa gtcttctgca tcaccttgag tacggtcttt
     1441 gttgatatcg atgataccca attcttgacc aatcccttgg ttaacaagag cataagcata
     1501 agcagaacct acggcaccgt caccgacaag gattactttt ttgtgttgtt tagttgcagt
     1561 cattgctaaa catctcctta gttttatttg gggatgactt catcccgacc ataatacatt
     1621 ctagcatgtt ttaagctttt tgtcacttgt gaaagcgcct ttgaaaatgt ttacatgcta
     1681 gtttccaaca aagaaattta ttcgccaata tggtataata gaagcacaat gactgttgaa
     1741 agaggcatat aatgcaagat aaaaatttag taacggttaa tctgaccaat gagatgaaat
     1801 catccttcat cgactatgcg atgagtgtta tcgtttcacg tgctctgcct gatgtacgtg
     1861 atggtctgaa gccggttcat cgccgtattt tgtatgggat gaatgaatta ggaattacac
     1921 cggataaacc acataaaaaa tcagcccgta tcacagggga tgttatgggt aaatatcacc
     1981 cgcatggaga tagtgcgatt tacgaagcaa tggttcgtat ggcacaatgg tggagctacc
     2041 gtcacatgtt agttgatggt cacggaaact ttggttccat ggatggcgac ggagcagccg
     2101 ctcagcgtta tacagaagca cgcatgagca agattgcgct tgaaatgctt cgtgatatta
     2161 acaagaacac ggtaaatttt gcagataact atgatgcgag tgaacgtgag cctgaggttt
     2221 tacctgctcg tttccctaac cttctggtta acggaacgac tggtatcgcc gttggtatgg
     2281 ctaccaacat tcctccacac aacttgggtg agactatcga ggcagttaag ctggttatgg
     2341 acaatcccga ggtaacaaca cgtgagatta tggaagtctt gcctggacca gattttccga
     2401 caggtgcctt ggttatgggg aaatctggaa ttcaccgtgc ttatgaaact ggtaaaggtt
     2461 caatagttct tcgttcccgc acagaaattg aagaatatgg aaatggtcgt gaacgcattg
     2521 ttgttacaga gtttccatac atggtcaata agtccaaggt tcaagaacat attgttaaat
     2581 tagtccaaga aaaacgtatt gaaggaatca ctgctgttcg agacgaatcc aaccgtgagg
     2641 gtgtacgatt tgttatcgaa gttcgtcgtg atgcttctgc taacgttatt ttgaataacc
     2701 tattcaagca aacacagctt caaaccaact ttagtttcaa catgttggcc atccaaaatg
     2761 gtgtaccgaa aatcctttct gtacgtcaga ttctagagtc ttatattgaa caccaaaaag
     2821 aagtagtcac tcgtcgtaca cgatttgata aagaaaaggc tgaggcgcgt gctcatatct
     2881 tagaaggctt gctgattgct cttgaccata ttgatgaagt cattcgtatt atccgtaatt
     2941 cagaaactga tgctattgct caagctgaat tgatggagaa atttgacctt tctgtacgtc
     3001 agagtcaggc tattcttgat atgcgtcttc gtcgtttgac aggtttggaa cgggacaaga
     3061 ttcaatcaga atatgatgag ttagtggcgt tgattgcaga tttggctgac attctagcta
     3121 agcctgagcg tgttgttcag attattaaag acgaattaga agaagtcaaa cgtaaatatg
     3181 ctgacccacg ccgtacagaa ttgatggttg gtgaagttct atcccttgaa gatgaggact
     3241 tgattgaaga aacggatgtt ttaatcacct tgtcaaacca aggctacatc aagcgtttgg
     3301 cacaggatga gtttcaagct cagaagcgtg gaggacgtgg ggttcaagga actggcgtca
     3361 aggatgatga ttttgtacga gaattagtct ctacaagcac tcatgatcgc ttgctcttct
     3421 tcactaataa gggacgtgtt taccgtctca aaggctatga aatcccagaa tatggtcgta
     3481 ctgcaaaggg acttccagtg gtcaatttgc ttaaattgga agaaaacgag gcaattcaaa
     3541 ccattatcaa tgtaactaag gaccaggagg cagatagcta tctcttcttt gcaacccgcc
     3601 aaggtgttgt aaaacggact agtgtgtcag aatttgccaa tattcgtcag agtggtctga
     3661 aagctttgaa tttgaaagaa gacgatgaac tcatcaatgt tttcttaaca aatggtcaag
     3721 cagacattat tatggggact aagttcggtt attctgtacg tttcactgaa acagatgttc
     3781 gtaatatggg ccgtactgca acaggtgttc gtggtatcaa tttacgcgag ggagaccagt
     3841 tagttggcgc tactatgatt tctgatgacc aagaagtact cgtattgacg gaaaaaggtt
     3901 ttggtaaacg tacaccagcc agtgagtatc caaccaaagg tcgtggtggt aagggtatca
     3961 agactcttaa agttgctgat aagaatggtt ctctggcagg tttgacaacg gtatctggtg
     4021 atgaagatat tatggttatt accgatactg gtgtgattat tcgtacaagt gttgccaata
     4081 tctctcaaac tggtcgttca accatgggtg taaaagtgat gcgcttgaat gatgaagcga
     4141 aaatcatgac ctttgccttg gtagatgctg ccgaaattaa agaagaaaag gaataaataa
     4201 tcatgtcaaa acgtgaaaaa aagaagaagc gtaaaggttc tttttggcgc aattttttaa
     4261 cagttgtatt gatactgatt tccttggcat tgatatttaa tacctctatc cgtaatttta
     4321 ttatcggctg gaatacgaat aaataccaga ttagcaatgt tacgacagag gatatcgaaa
     4381 agaataaaca ggctgagaca acatttgatt tcgaacaggt tcagtctatt tctacagaag
     4441 ctattttagc agctcagtgg gatgcacaac gcttacctgt aattggtggg attgcggttc
     4501 ccgagcttgg tatcaacctt cctattttta aaggggtctt caatacttcg ctcatgtatg
     4561 gagctggtac catgaaggaa aaccaagaga tggggaaagg aaattatgcg ttggctagcc
     4621 accatatttt tggtgtaact ggtgcggcag atgttctctt ttcaccgctt gatcgtgcta
     4681 aaaacggcat gaaaatctat attactgaca agaccaatgt ttacacctac gttattgata
     4741 gtgtggaaat tgtttcgcct gaaagtgtct atgtcattga tgatgtagaa ggacgtacag
     4801 aagttacgtt agtgacatgt acggactatt atgctacgca acgtattgtt gtaaaaggag
     4861 ttcttgaatc aaccactcca tataatgaaa cggcaaaaga catcttagat tccttcaata
     4921 agagttataa tcagtatgat tatggacaat aaaacatcta tatattaagg ataaagaagt
     4981 ggtataggac tgaggagata atctttggtt ttatatggct tctttttttg caagatttta
     5041 cgaatagata ggtgaggagg aaatgatgta tcaaattgaa ttcaaagaag aggccattct
     5101 acctagagaa cgtttggtag aagttggagc tgagcgtctc agtaatcagg aactactagc
     5161 aatttttatt agaactggga caaaaaaaga acctgtttct attctctcca ataaattatt
     5221 gaatcgtttg gagagtttag cggctctaag agaactatca attgaagaat tgcaaagttt
     5281 gactggaatt gggcgcgtca aggcgattga aattaaggct atgattgaac ttggaaaacg
     5341 tatcaatcaa tcagagctgc tcttaaatga gagaattttg ggaagtgaga agttgggacg
     5401 taagatgatt catgaaatcg ggcataagaa gcaggagcac ctagttgcac tttatctcaa
     5461 tacacagaac cagattatca gtcaaaaaac gatttttatt gggagtgtta atcgtagcat
     5521 tgcagaacca cgtgaaattt tgcattacgc agtaaaatgt atggcaacat cgattatcat
     5581 cgttcacaac cacccgtcgg gttcagtaca acctagtagg aatgatttgc tatttacgga
     5641 gaatttaaaa gagtcctgcg aaaagctagg actggtatta ttagatcatt taattgttgg
     5701 aaataaggat tactattctt ttagagaaga aagtgagtta ttttaaagtt tattgacaat
     5761 atagtggaag agttcttttt caatctcacg tttaccgtag agtagttcag gatgccattg
     5821 aacccctaaa aatttagttg ggtaggctga atgaactgct tcaataatct gttgattatc
     5881 tgatagtgca atgatttcta agtcaggggc taagtctttg atagcctgac ggtgaaatga
     5941 gttcacactc ggatcggact catatatgtc gtaaagtgga ctattttgag tcagttgaat
     6001 cgtttgacta acttcttgtc catctatgtc ttgccaatgt tcagaaattg attgatgaag
     6061 gctaccacct tgagctacat tgtagagctg tagacctcgg cagataccga agataggttt
     6121 ttgattttct tgggcagccc ttatgatggc caattcaaac tcgtcacgct ttggtagata
     6181 attatcgctg tcaatagttc tttcttcgcg atagtaactt ggctggacat tttgtcctcc
     6241 tgttaggatg agtttgtcaa taagatggat ataatatttt gccaaatctg gctctgtaac
     6301 aggaaggatg agtgggagcc cgccagcatc ttcaatagct tgtacgaggc aagtttgggt
     6361 ataagaaagc aggggttctg tatgatcccc tgttttatat tcatttccag aaataccaat
     6421 aactggtttt ttcattgctt atccttttcg cataaagtag agtagtgtct gtaattcact
     6481 agtcaaatca acatactgga caacgacatc ttttgggaca gacaagttaa ctggtgaaaa
     6541 gctcagaatt cccttgacac cagcttcaac aagaatatcg gtcacttctt gtgccttgac
     6601 gctgggtacc gttagaatgg ctgtctgaga gtttgcttcg ctgatacgtt ccttgatctc
     6661 tgagattgca tggattggga tattttcatc tgtcgttcca acagctggat tatcatctgc
     6721 ttcaaaagcc atgactattt tcattttatt tcgttcgtgg aagcgatagt gtagcaaggc
     6781 ccgccccata ttcccaacac caacaagcat aacattggtg atggaggtat catttagaat
     6841 atctgcaaag aaatccatca actctttgac attgtagcca aagccacgac gcccaagctc
     6901 tccaaaatag gaaaaatctc ggcggacggt agccgaatcg attccgatag cttcagcaat
     6961 ttcttttgaa ctagcttttt caatatttcc agcatagaaa cgtttaaaaa tgcgatagta
     7021 gagggataga cgtttggcag tagcgcgtgg aatatcagat tttttatcgt ttttcacttt
     7081 agactccttt atttcgatag aatacgcttt atcgattatt tatatattaa acagttcttt
     7141 attctattct agtccaagtt tgtgaaaaaa tcaacagatt gattttaaaa aaagattaaa
     7201 caggaaactg tctaatctag tgtttgtaaa tagcgataga gatctccgat tgtccctttg
     7261 taatccagag cttgtccgtc ttgtgtaatg ctggtaactg gataatagtc tgggagggtt
     7321 aggcaaccgg aaaaagccag aatggttgca tccttattct caaatgtttg gatacgttct
     7381 tgcttgtaag cgtcaagaca gtgaatttca atcatactaa ctctcttttg taaagatgtc
     7441 acggtcaatc aagctatcca tgaggaaata gaatttttct tgaataacct tgttactttc
     7501 tggcttaaaa atgtttacca gatgaagacc agacttgtcg gtaatgttaa ttttaaaacc
     7561 agttaggtct tgattgataa taattttcaa gaggaatcct tggtttgaat ttggaatctc
     7621 ttccagaaga cgtgtaagct gatagtgacc tgctttggtt tgttcaaagg tgttatcacg
     7681 gagtgtatat tttttgatag cagggctaat gtgataagta aaattacaat cttttaaggt
     7741 aactgattgt tggaatgcca ttttaacctc caagtatttt ctttagttcg atgataagag
     7801 tatctatctc ttcttttgta ttgatttcag ataggctgat acgaatggat tctttcagac
     7861 gatgggagtc ttttccatac atagcttcta aaacatggct attttggaca acacctgccg
     7921 tacatgcaga accactagaa acagcgatac ctgctaaatc tagttgcata aggagttgtt
     7981 cattaagttt accaggaaaa ccgatattaa ggacatgagg gagatgaggt ccagcctgat
     8041 tgagataata gtctaagcca gacaatccct cgataatatg ttgtcgcagt tcactgacat
     8101 gttgatgatg cgtatctaag gactcatatt gttctgttag ggcagtcgcc atagcagcaa
     8161 tagcagggag attctcagta cctgctcgat gttgctgttc ctgctttccg ccgtggatca
     8221 aggaatcaaa tttatgaact ttactataca ggaatcccgt tccctttgga ccatggaatt
     8281 tatgggcaga ggccgcgagc aagtcaattc cgtagttaga tggagatatt gcgatttttc
     8341 caattgcttg gacagcatct acatgaaaga cggcctgatg atcggctagg agttctccaa
     8401 tttccttgat gggaagtagc tgaccggtct cattgttagc aaacatgacg ctgactaaaa
     8461 tagtatctgg ccgaagggca tcctgaattt gttgagcggt gattacttgg tcgactggtt
     8521 ggatataggt tacctcgaac ccaaaccgtt cctctagata ctgcatggta tggaggacag
     8581 catggtgttc gatagccgta gtaatcaggt gttttccttt ggattgatgc gcaagtgcat
     8641 agccttgaat agctaaagta tcagcttcgg agcctccaga tgtgaaaata atttgttcgg
     8701 gagaggcgga gagaatctga gcaatctctt gacgactttg tctaagacgt tgattggctt
     8761 tccgaccgct ttgatgaata ctggaggggt ttccataatt ttcttgagcc acttccatca
     8821 tccgttggag tgcagtagga gatagagcag tggttgctgc attatctaaa taaatcaaag
     8881 agttactcct agttttcttt ttcaggacta gtaaatttga aaagtgggct gagtggctga
     8941 tgttcttgga tgcggtggat ggcatctgca atcaagtcac tagctgttag gaagacgata
     9001 tttttaggat gctgttcttt gcttgcaaca gaatcggtaa ctaatatttc tttgattggt
     9061 gcttcatcta agagttgggt tgcagttcca gcaaagagac cgtggctggc aactgcataa
     9121 atttcagtag caccaccttc ttggacaatt tttgaagctt gtgagaaagt gcgaccagta
     9181 ttgagaatat cgtccaccaa aatagctttt ttaccagcaa catcacctat aatatagcct
     9241 tcagaacgct cagaatcatc ttgagcgtag tcgataattg caatcggtgc atttagaaat
     9301 tcagcgatat tacgcgcgcg tttgatacca gaatttttag gactcacaat gacgacatct
     9361 tcaccgcaga gtcctttttc catgtagtag gctgcaaaaa gtggttctgt aaggagattg
     9421 tctactggaa tatcaaagaa accttgaatt tgtgaagcgt gcaagtcaag agtcaataca
     9481 cggtcgacac cagctttaac cagcatattt gcaactaatt tagctgtaat tggttcgcgt
     9541 ggagaagcgg tacggtcttg acgagcatag ccgaaataag ggatgacagc tgtaatggtg
     9601 ttggcactag cacgtttaca agcatcgatc ataatcaata attcccacaa gtgattattg
     9661 accggatagc ttgttgattg gataatgtag acgtctacgc cacgtacaga ttcttcaatg
     9721 ttaatttgaa tctcaccatc agaaaactga cgagaagaca gctttccaag agggattcca
     9781 gcagaatcag caattttttc agcaatatca cggttgctat tgagtgagaa taatttaatg
     9841 ttatgttcac cgagtggttg taccatttta aagtaactcc ttttacacac tgagctttta
     9901 tgatgacata aaagtatttt ttacgataga tagttctatt ctatcaaaaa aatgaaaaat
     9961 tttcagtagt gaaactaaaa aatcaacctt agttggctga ttttttatag ttatttcatg
    10021 tgggcagcag ttcgtgcgac cttactacta gcatatttga attgaattga tttcttctta
    10081 aggaattcat caaaaagaat tttccctgca tctgcatcgg tcacttcgac ttctaattcg
    10141 taatcttttt gtcccacata atcattttca tcgagggcca taaggcctat agatgtctgt
    10201 ttctcatagc gttttgtcgt gaggattccc cagacagcaa gtttatcaag tggaatttcg
    10261 gttgcagaaa taatatcaga aatttgtcct gctggtagtt gaaaattttc cagtaaatca
    10321 tgagcagttt ggatgtctag aacttggtta tattcttgat tgccgacttc ttgtggaatt
    10381 tttaaggtta gttctgctga gtcctcaaat gtacgaatac ggagtgaaaa gcggtgattc
    10441 ttcatatcta ggtctggggt atcaatgtag tggtttgttt gaagaattgg gctcacatct
    10501 gaaaagtcag taagcaggcg tttgtattcg gatttggtca gtaaggtttt atactcaatt
    10561 tcaaggtggt ttttcatgca gtaaaccctt tcaatgtgtt ataatagatt agtctttgtg
    10621 ttattctaat acaatttaaa agttttgaca agaaaggggc cgtatggact ttaactggga
    10681 agaattttta gacccctata ttcagacagt tggtgaattg aaaatcaaac tgcgggggat
    10741 tcgcaagcag tatcgcaagg ctaatcggca ttctccaatt gagtttgtca caggacgggt
    10801 aaaaccgatt gaatccatta aagaaaaaat ggctctgcga catattaaat tggaaaatct
    10861 tgctcaagat atgcaagata ttgccggtct tcggattatg gttcaatttg tggacgacat
    10921 tgaagaagta ttagctattt tgcggaaacg caaggatatg caaattgtgc atgaacggga
    10981 ttatatcaat aatatgaaag cctctggcta ccgttcatat catgtggtga ttgagtatcc
    11041 tgtggatact ataaatggca acgagacggt tctggcagaa atccagattc gaaccctttc
    11101 catgaatttc tgggctacaa ttgaacattc tctgaattat aaatataagg gaaattttcc
    11161 agaagaaatc aagaaacgct tggaagttac agcaaaaatt gcctatgaat tagatgagga
    11221 gatgcgaaaa atccgcaacg acattcaaga agcgcaggcc ttatttgatc ctgcctatcg
    11281 caaactgaat gacggtgtgg gcaacagtga tgatacagat gaagaataca ggtagaaaaa
    11341 aaatcgctct gctcgctagt cgaaatccta agagtgaagc ggtgtccaaa gaattatgga
    11401 caaagttaaa agaagcaaat tttattttga ctcctaaaaa tccagatatt gtcatttcta
    11461 tcggtggaga tgggatgctt ttatctgctt ttcacaaata tgaaaagttg attgatcgtg
    11521 tacgttttgt aggaattcac acgggtcatt tggggtttta tacagattat cgcgattttg
    11581 aagtggacaa gctgattgaa aatcttaagc ttgatacagg tgctagagta tcgtatccga
    11641 ttttaaatgt taaggtcaag atgacagatg gtcgcattgt cgaggcgcgt gccttaaatg
    11701 aggcgacagt aaaacgtctt tctaagacca tggtcgcaga tattattatt aacaatgtgc
    11761 ccttcgaacg cttcagaggg gatggaattt cggtttcaac gccgacaggc tcaacagcct
    11821 ataacaagtc attaggtggt gctgttttac atccaacaat cgaggcattg cagattgcag
    11881 aagtggcaag tttgaacaat cgtgtttacc gcaccttggg ttcatccgtt gttgtcccta
    11941 aaaaagacaa gattgtgatt gaaccaaagc atagtgaccg ttactcgatt gcggtagaca
    12001 ataagacttt tgtctatgat agtattgaaa gtatagagta tcagatagac aatagtaaga
    12061 ttcattttgt tgcaacaccg agtcatacta gtttttggaa tcgggttaag gacgccttta
    12121 ttggagaggt ggagtaatgc ggtttgaatt cattgctgac cagcatacga aaataaagac
    12181 tttccttaaa aaacatggtg tctctaaagg actattagcg aagattaagt ataccggtgg
    12241 caatatctgg gtcaacgata ttgagcaaaa tgcaacttac ttgcttgata ttggagatag
    12301 ggttacaatt gacatacctg ctgaagagga tttgactggt agtttgaagc cgattttctt
    12361 tccgttggat attgtctatg aagatgatca ttttctgtcg ataaataagc cggttggcta
    12421 cgcctccatt ccttctgcct tgcattcatc cacaatcgca aattttgtaa aaggttatct
    12481 tattgagcag aattatgaga ataagcaggt tcatattgtg actcgtctgg atagggatac
    12541 atcaggcgtc atgctgtttg ccaagcacgg ctatgcccat gcacgactcg ataagcagtt
    12601 acaggccaag cttattcata aacgttatta tgctttggtg aaggggaatg ggcagctaga
    12661 agagacaggg gatattattg cccctatcgg tcgtccagaa gatagcatca ttacccgttg
    12721 tgtgacaaag aatggaaaat atgcccatac ttcctatcgt gttgtgcaat cgtggggaga
    12781 tattcattta gtagatattc aactacatac tgggcggact catcagattc gtgttcattt
    12841 ttctcacatt ggatttccgt tgttaggaga tgatatgtat ggtggaagtt tggaatgtgg
    12901 gatagaacgt caagccttac attgtcataa tttggccttc gacaatcctt tttcagccga
    12961 aaggattgat ttggaagcac cgcttcctga ggattttcag gctgttatca atcagttaat
    13021 aagtaaataa atttaaggag tttgttcatg aaaatttttg agcaattagc tacaaagcta
    13081 gagggtaaaa aagtacgtat cgtattgcca gaaggtgaag aaccacgtat cttgcaggcg
    13141 acaaaacgtt tggtaaatga gtcggatgta gtacccgtct tgcttggaaa cccagatcgt
    13201 attaagattt accttgacat tgaagggatt acagaaggtt ttgaaattat tgacccagct
    13261 cattatgata gatatgatga gatggtagct gctttggtag agcgccgtaa aggtaaggta
    13321 tcagaagagc aggcacatga gttgttgaaa gatgttaact actttggtgt tatgttggta
    13381 tatatgggcg tagtagaagg tatggtatct ggtgcgattc attcaaccgc ttcaacagtt
    13441 cgtccagctc ttcaaatcat taaaacagag ccaggtgtat cgaaaacatc aggtgccttc
    13501 ttgatggtaa aaggtgaaga gcgttatatt tttggtgact gtgctatcaa cattgatcca
    13561 gatgctcaaa ccttggcaga aatcgctatc aactcagcac gtactgctaa aatgtttggt
    13621 attgatccca aagtagcgat gctcagctat tctacaaaag gctctggtgc tggtcctaaa
    13681 gtagataagg ttgttgaagc tacaaaaatt gctcaaaaat tgcgtcctga gttggatttg
    13741 gacggtgaat tgcaatttga tgctgccttt gttcctacca caggtaaatt aaaagcccca
    13801 ggttcgagtg ttgctggtca agcgactgta tttatcttcc caggtatctc agctggtaat
    13861 attggttata aaattgccga acgtatgggt ggatttgcag cggtaggtcc tgttttacaa
    13921 ggtttgaatc agcctgtaaa cgacttgtct cgtggttgta accctgatga tgtttacaac
    13981 ttgaccttga ttactgcggt tcaggctttg gagcaccgtt aatatgaaat ttttatcacg
    14041 atattttaag gactatatta aggaatccat attaggtc
//
LOCUS       DAZQCJ010000047        14039 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000047 DAZQCJ010000000
VERSION     DAZQCJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14039
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.048"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(55..510)
                     /locus_tag="ACHU66_001252"
     CDS             complement(55..510)
                     /locus_tag="ACHU66_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFR3389539.1"
                     /translation="MELHSHFNAENQTAFEHVIQHLKEKGVRITETRKAVVAYIIESD
                     DHPSAEMIYQDLLPNYPNMSLATVYNNLKLLLEEGFVTKLKRTNDNTTYYDFMGHEHL
                     NVICEVCGKITDFMDVEIPSLKKEAHTQTGYKITKEVLSIYGICPDCQG"
     gene            677..1426
                     /gene="truA"
                     /locus_tag="ACHU66_001253"
     CDS             677..1426
                     /gene="truA"
                     /locus_tag="ACHU66_001253"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR3389540.1"
                     /translation="MTRYKAIISYDGHDFSGFQRQTHARTVQEEIEKTLVRLNSGQPV
                     TVHGAGRTDAGVHAYGQVIHFDLAGSRDVEKLRFALDTQTPEDIDVVQVEQVADDFHC
                     RYAKHSKTYEFLVDIGRPKNPMMRHYATFYPYDLDLSLIEEAIQDLVGTHDFTGFTAS
                     GTSVEDKVRTIKAASMEYDQRRQFLIFTFSGNGFLYKQVRNMVGTLLKIGNGRMPVGQ
                     IKRILAEKDRGLAGPTAAGNGLYLKEIIYED"
     gene            1416..2177
                     /locus_tag="ACHU66_001254"
     CDS             1416..2177
                     /locus_tag="ACHU66_001254"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR3389541.1"
                     /translation="MKTKYILALSGNDIFSGGGLHADLTTYTVHGLHGFVAVTCLTAM
                     TEKGFEVIPTDAAVFAQQLASLKDVPFSAIKIGLLPTVDIAEQALAFVKAHQEIPVVL
                     DPVLVCKETHDTEVSQLRDELLKFLPYVSIITPNLAEAQLLLQKDIETVEEMKQAAVA
                     LYDLGAKAVVIKGGNRLGGSEAIDVFYDGQEVVVLQSPLLSENNVGAGCTFASSIASQ
                     LLLGREPFEAVRLSKDFVYAAIEKSDDYGVIQYEK"
     gene            2167..2652
                     /locus_tag="ACHU66_001255"
     CDS             2167..2652
                     /locus_tag="ACHU66_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR3389542.1"
                     /translation="MRNKKTQELVLLAILTALSLVLAFVYVPTATGFVTLLDVGVYFT
                     AFYLGKKEGAIVGGLAGLLIDFLLGYPQWAFFSLLFHGAQGYFAGWTGKKRIFGLVLA
                     TLSMVGGYYLASRIYFNDLKAIESVLSNTLQNTVGLVVGYILAQAIERIGAVNHVIKG
                     N"
     gene            2633..3199
                     /locus_tag="ACHU66_001256"
     CDS             2633..3199
                     /locus_tag="ACHU66_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFR3389543.1"
                     /translation="MSLREIKDQTQQVVQEVLELSNLQKGQIFVLGLSSSEVIGGHIG
                     KNSSLEVGEVVVETILEILDQKGIYLAVQGCEHLNRALLVERELAIQKDLEIVNVLPT
                     LHAGGSGQLAAFKYMKDPVEVEFITAQAGVDIGDTAIGMHIKHVQVPIRPSLREIGQA
                     HVTALASRPKLIGGVRAAYQEDKIRKGS"
     gene            3199..3813
                     /locus_tag="ACHU66_001257"
     CDS             3199..3813
                     /locus_tag="ACHU66_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HFR3389544.1"
                     /translation="MKEFTVKDLVWTRPPRQFTISDEEITITTHPQTDLWQKTYYHFV
                     NDNAPVLQMETEEDYFSFTVKTDFAGSHTRFDQCGIVLYQDSENWLKCSIEYENDDFQ
                     HLGSVVTNHGFSDWATTEISSQVKSIWFRLSRRGADYCFENSEDGIHFKQMRVCHFHQ
                     GTGKIAFGLYACSPENSSFTARFTNMELSDCKWLPHDGQEPDEI"
     gene            4086..4517
                     /locus_tag="ACHU66_001258"
     CDS             4086..4517
                     /locus_tag="ACHU66_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFR3389545.1"
                     /translation="MDTKFSVALHILTMISESKEILSSQALAESVGTNASYIRKVIAL
                     LKNAGLITSHQGRSGYQLSKSPKDISLLEIYLATQEVEHIRLFQIHQNANLTCPVGQH
                     IEGAMVPIFSSVEQDLENQLSRQSLDNVIDNLYQSAQETRI"
     gene            4597..5598
                     /locus_tag="ACHU66_001259"
     CDS             4597..5598
                     /locus_tag="ACHU66_001259"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFR3389546.1"
                     /translation="MKAAQHTSYNKNKITLNLTEIAKPQIKPNQVLVKVTAAGVNPLD
                     NMISRGDVKLIVPYKLPQTAGNELVGLIEEVGSSVTTFVAGDRVFGRLPLDSIGAFAE
                     YVAVDAQALAKVPAYLTDEEAAAVPLTALTIMQALDLMGAEAGKTIFISGGTGGVGGM
                     AIPIAKAKGLTVITNGDGASGERVLKLGADRFIDYKTEDYTKTLSNVDYVLDTLGGAE
                     TEKQMSIMEKGGQLVSLRAMPNGEFAKRMNLPKWKQILFGLAGRSFDKMAKKYGVHYH
                     FIFVESNGRQLQEVADIFSKLEIKPSIDTVYPFEEVNAALDKVANGRSRGKTVLSFN"
     gene            5624..6469
                     /locus_tag="ACHU66_001260"
     CDS             5624..6469
                     /locus_tag="ACHU66_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR3389547.1"
                     /translation="MSYITTKNQYITVSNNKIAYRELSKGKSDIPLVMLVHLAATLDN
                     WDPKLLDLLAEQHHVIVLDLPGVGASQGKVAPTIPGMAEQAVSIIKALGYEKINLLGL
                     SMGGFIAQEIVRLDSQLVNRLILAGTGPRGGFEVDKVTGKTFRYMLKAGLERVDPKRY
                     IFYNHDEAGRLEAEKVLGRMGQRSAAHADKDMNVPGFLTQLKAIKRWGREPQDDMTFI
                     TQPTLIVNGDKDMQVPTENSYTMHEKIKNSQLIIYPNAGHGSIFQNVEDFSKALLAFL
                     EETNA"
     gene            6462..7331
                     /locus_tag="ACHU66_001261"
     CDS             6462..7331
                     /locus_tag="ACHU66_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFR3389548.1"
                     /translation="MPKTILITGSTDGIGKHLAMKLASEGHEVILHGRNSEKFRVALS
                     DIQLKTGNKKIHGYLADFSKLADVYRFSQEIKRDFEQIDVLVNNAGAYFGDARVATAE
                     NIEMTFMLSVQVPYILTTELLPLLEKAVGRVIHTSSFMHHFAQTRGLDFGLEQSYSAA
                     MAYNNAKLYTIWLAIAQAEALEKQGSSVTVNAYHPGLIATNLGNDGVKRNLRSRILTS
                     LMKPFSKDLDQGIETGYYLSLSPEVAGQSGRYFAEKKVAKVSLKGFDVPKSQALLAYL
                     DKKIQVFKESYRD"
     gene            7324..8535
                     /locus_tag="ACHU66_001262"
     CDS             7324..8535
                     /locus_tag="ACHU66_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HFR3389549.1"
                     /translation="MTKILLENLQLPNGQVLDNRFFKSAMSETMADSQGAPSDDLIAL
                     YDFWAKQGMGVLVTGNIMVDGRYLGEPGNVVLDSDDHLAQFRKWAAVGKKNGVPIWLQ
                     LNHPGKQMYRSINQTPIAPSAIPISGGSASAFRPPREMTVFDIKEARDKFIAAGLRAK
                     EAGFTGVQLHAAHGYLINQFLSPADNQRTDRYGGSLDNRMRFLVEVYQGLRDKVGPDF
                     TIALKLNASDFKEEGFGFEDCKHVVKTMSDLGIDLIEISGGNYETPVFGSEYENGAGF
                     VTYALALADLTSVPIVSTGGFRKVTQMEEAIKDGVSMIGLARPFVLRPSLVNDYRQVG
                     DVQLTTPRLTTGLPKLDKALGPIIGVSYYEAQMKRLAMGKSVTVSQHAWPYLLQTIKA
                     HGLSALKPRRK"
     gene            complement(8802..9311)
                     /locus_tag="ACHU66_001263"
     CDS             complement(8802..9311)
                     /locus_tag="ACHU66_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR3389550.1"
                     /translation="MERVKRKESLFVESVVEALWELLEDKSFEKISVSELVERAGIGR
                     VTFYRNFSNKEEVLERSFNMELQAWLSERQIDLSDWSDAHLLLALRQFFDFWYEQQDK
                     IRLLTTNHLDYLLEEVLDRYFKERLGDLTDDFHLQFIVGGFFRVLKTWVARGCKESPD
                     DIMRLMGEQ"
     gene            9454..10572
                     /locus_tag="ACHU66_001264"
     CDS             9454..10572
                     /locus_tag="ACHU66_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HFR3389551.1"
                     /translation="MKKVWKIVAAVTVVLALLGFIAYKKTFGWSAPELVAQTPQVNEW
                     YRLSPEGVVDSQGNQAHGLIRTGKEKNKVMVYFFGGGVSINEETASGGTRYFATTTGH
                     QDYVATWGIGSPQEDNPFKDWTMIVLPYGTGDFHAGTQNFSYVDDNGKEQVVHHQGYS
                     NLMSILAAAKPHVGNPDTLLVTGFSAGGFATSLLADDVISHFPTAKNVTVAVDSSFLR
                     HDNWKSIAENVWKTPTSISDRLHSDNIVLDSLVALHEKRGDSVKILFDISYRDGILQQ
                     YQAYIDQGKLADATEESSDHFQKELKKMIQELQSKIDGVGIFIWNYGQDEKTTATQHT
                     TINFSTFFTPMSKDKSVAEWLDDAVNGQVNSYGLELLE"
     gene            10582..11601
                     /locus_tag="ACHU66_001265"
     CDS             10582..11601
                     /locus_tag="ACHU66_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR3389552.1"
                     /translation="MKKFKKIILWTLGIVASLGIIAYVSVTNHPQLVVGVIQSFMYKE
                     SSPNSYSPLYDPIDGLKENGQYLKSEIAYSKDYPNSFLDITYPDQNLEADRPTLFYFH
                     GGGFFGGSKNMGDPLAASQATYLIDDIVSKGYNVVNVDYALVPDYHFPTPVIQMNQAI
                     AYMKEHAEEYHLNMDNVVLMGSSAGAIMVAQYGSLLANNDYAQLLNIEPSIAKEHVKA
                     LVIDDAPLDYAKFSLSTKLLVGNYVSETIYLSEEEINAYNPISHVNKDYPASFLLGSE
                     YRTDMVSLNKALKQAGVENELVDPLAEEGLEKPHGFVANLRTDPVSAKAFERLITFID
                     DRTKE"
     gene            11823..13106
                     /gene="tig"
                     /locus_tag="ACHU66_001266"
     CDS             11823..13106
                     /gene="tig"
                     /locus_tag="ACHU66_001266"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR3389553.1"
                     /translation="MSVSFEAKETNRGVLTFTIGQDAIKPELDRVFNKVKKDINLPGF
                     RKGHLPRAVFNQKFGEEALYQDVVNALLPAAYEAAVAEAGLEVVAQPKIDVVSMEKGQ
                     DWTITAEVVTKPEVKLGDYKNLAVSVEATKEVTDEEVDAKIEAARNNLAELVIKEGPA
                     AEGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEAQLVGHAAGEEVNVEVT
                     FPEDYQAADLAGKPALFVTKIHEVKAKEVPALDDELAKDIDEEVETLDELKAKYRKEL
                     EAAKEVAFDDAVESAALELAVENAEIVELPEEMIHEEVHRAINEFLGGMQQQGISPDM
                     YFQITGTTREDLHKQYEADAEKRTKTNLVVEAVAKAEGFEATEEEINKEIEDLAATYN
                     MEVAQVRSLLSPEMLKHDIAVKKAVEVITSTATVK"
     gene            13154..14039
                     /locus_tag="ACHU66_001267"
                     /pseudo
     CDS             13154..14039
                     /locus_tag="ACHU66_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643060.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4062 a         2856 c         3264 g         3857 t
ORIGIN      
        1 ataattcaaa ggaaaataaa aaaagcgaca gaaagccgct ttgatattcg tcttttagcc
       61 ctgacaatca ggacaaatgc cgtagatgga tagcacctct tttgtaattt tgtaaccagt
      121 ttgggtatga gcttccttct tcaagctcgg gatttctaca tccatgaaat cagtgatttt
      181 tccacagact tcacaaataa cattgaggtg ctcgtgcccc ataaagtcgt aataagtagt
      241 attatcattg gtccgtttca atttggtcac gaacccttct tccaatagaa gtttgaggtt
      301 gttgtaaaca gtcgccagac tcatgttggg gtagttaggt agtagatctt gataaatcat
      361 ttcagcacta gggtggtcat cgctttcgat aatgtaggca acgactgctt ttctagtttc
      421 ggtaatgcgc acaccctttt cttttagatg ttggatgacg tgttcaaaag cagtttggtt
      481 ttctgcattg aagtgagaat ggagttccat agtttcctcc tttcgttatt attttataaa
      541 aattctaaga tacaatattc agtttcatta tagtgtattt gggaggttgt gtcaattttg
      601 agacaggaaa tctgtcatag gacagtttgg tgtctcatga cttgtttaca tacttttcct
      661 gtgctacaat caaaacatga caagatataa ggcaattatt tcctacgacg gacatgattt
      721 ttctggtttt cagcgccaga cccatgcccg tacagttcag gaagaaatcg aaaaaacgtt
      781 agtaagattg aatagtggtc agccagtaac cgttcatggt gcaggtcgta cagatgcagg
      841 ggtacacgcc tacggtcagg ttatacactt tgacttggct ggcagtcgag atgtggagaa
      901 gctccgcttt gccctggata cccaaacccc tgaggatatt gatgtggtcc aggtggagca
      961 ggtggcggat gattttcatt gccgctatgc caagcacagc aagacctatg agtttctggt
     1021 ggatatcggg cgacccaaga atcctatgat gcgccactat gcgacctttt atccttatga
     1081 tttggacttg agtttgatag aagaagccat tcaggacttg gtggggaccc atgactttac
     1141 aggctttacg gcttcgggga catcggttga ggacaaggtg cggaccatta aggcggcaag
     1201 catggaatat gaccagcggc ggcagttctt gatttttaca ttttcaggca atggtttttt
     1261 gtacaagcag gtgcggaata tggtcgggac cttactgaaa attggtaacg gtcgcatgcc
     1321 tgtgggacag attaagcgga ttttagcaga aaaggatcgt ggtttggcgg gaccgactgc
     1381 ggcaggcaat ggcctctatc taaaggagat tatctatgaa gactaagtat attttggcgc
     1441 tttcgggcaa cgatattttc agcggtggcg ggctccacgc agatctgaca acctacacag
     1501 tccatggtct gcatggcttt gtggcggtga cttgcttgac agctatgacg gaaaaaggtt
     1561 ttgaggtcat tccgacagat gcggcggtgt ttgcccagca actggctagt ttgaaggatg
     1621 tgcccttttc tgcgattaag atcggcttgc ttcctacggt ggacatagca gagcaggctt
     1681 tggcgtttgt caaggctcac caggagattc ctgtggtctt ggatccggtc ttggtctgca
     1741 aggaaacgca cgataccgag gtcagccagt tgagagatga actcttgaaa ttccttcctt
     1801 atgtcagcat tatcacacca aatctggcag aagctcagct attactccaa aaggatatcg
     1861 aaacggtgga agagatgaag caggcggcag tggccttgta tgacttggga gccaaagcgg
     1921 tggtcatcaa gggaggcaat cgtctgggtg gctcagaggc gatcgatgtt ttctatgacg
     1981 gtcaggaagt ggtggtgctc cagtcgcctc tgctgtcaga aaacaatgtg ggggcaggct
     2041 gtacctttgc atccagcatt gccagtcagc ttttgctggg aagagaacct ttcgaggcag
     2101 tcagactgtc caaggatttt gtctatgcgg cgattgaaaa atcagatgat tatggggtga
     2161 ttcaatatga gaaataaaaa aacacaggaa ttagtattat tagctatctt aacagctttg
     2221 agcttggtat tagcctttgt ttatgtgcca actgcaactg gttttgtcac actcttggat
     2281 gttggggtat attttacagc cttttacttg ggcaagaaag agggagcgat tgtcggcggt
     2341 ttggcagggc tattgattga cttcttgctg ggctatcccc agtgggcttt ctttagtttg
     2401 ctcttccacg gggctcaagg ttattttgcg ggttggacag gtaagaaacg gattttcggt
     2461 ttagtcttgg cgaccctctc tatggtgggg ggctattacc tagcatctcg gatttatttt
     2521 aatgacctca aagccattga aagtgttcta agcaatacct tgcaaaacac agttggtttg
     2581 gtagttggtt acatcttggc tcaggcaata gaaagaattg gtgcagtaaa tcatgtcatt
     2641 aagggaaatt aaagaccaaa ctcagcaagt tgtgcaagaa gtcctagaac tcagcaatct
     2701 tcaaaaggga cagatttttg tcctaggatt gtcctctagt gaggtcattg gtgggcatat
     2761 tggcaaaaat tccagtctgg aagttggtga ggtggttgtt gaaaccatct tggaaatcct
     2821 ggatcaaaaa ggtatctatc tagctgtcca aggctgtgaa cacctcaatc gtgccttgct
     2881 ggtggagcgg gaattggcaa tccagaagga tttggaaata gtcaatgtcc tgccaaccct
     2941 tcatgcaggt ggttctggtc agttggcggc cttcaagtat atgaaggacc cggtggaagt
     3001 cgagtttatc accgctcagg ctggtgtaga catcggcgac acagctatcg gcatgcacat
     3061 caagcatgtc caagttccga ttagacctag cctgcgtgaa attggacagg cccatgtcac
     3121 agctcttgcc agccgaccaa aactaatcgg cggagtccga gcagcctatc aggaagataa
     3181 gatacggaaa ggttcataat gaaagaattt acagtcaagg atttagtgtg gacccgtcca
     3241 ccgagacaat tcactatttc agatgaagaa attaccatta ccacccaccc ccagacggat
     3301 ttgtggcaga agacctatta ccattttgtg aatgacaatg ctcctgtctt gcaaatggaa
     3361 acggaggagg actatttttc ctttactgtg aagacggatt ttgcgggtag tcatactcga
     3421 tttgaccagt gtggaattgt cctttatcaa gattcggaga attggctcaa atgttcgata
     3481 gaatatgaaa atgatgactt ccaacacttg ggaagtgtgg tgaccaatca cggcttttct
     3541 gactgggcaa ctactgaaat cagttcccaa gtcaaatcca tttggtttag gctcagtcgc
     3601 aggggagctg attactgttt tgaaaattcc gaagacggta tccacttcaa acaaatgcgg
     3661 gtttgccatt ttcatcaagg gacggggaaa atagcttttg ggctatatgc ctgttcgcca
     3721 gaaaactcca gttttacagc tcgctttacc aatatggaat tgagcgactg caaatggttg
     3781 ccccatgacg gacaagagcc agatgaaata tagtgagagt cataggtaag tagtaacaga
     3841 aaaatagttg aaactcatat tcttcgaaaa tcaaaaagat ccgttgtcaa cttgccttga
     3901 agaactcctg ttcaatcttc gccttcgttt cctaggcgac ttttgatttt cattgagtat
     3961 caggaagtaa ctttcgacta gtaagagagt tacttttttt atcttttgat ccaaatcctt
     4021 gccaagaagt ctggcttgtg ataaacttgc tcctgtaagt gatacaagaa cagaaaggaa
     4081 accagatgga tacaaagttt tcagtagccc ttcatatact gaccatgatc agtgagagca
     4141 aggaaatact tagctcgcaa gccttagctg agagcgttgg caccaatgcc agctacatcc
     4201 gcaaggtgat tgcacttttg aaaaatgcag gcttaatcac ttcgcaccaa gggcgatcag
     4261 gctaccaatt gagcaagtcc cctaaggata ttagcttatt ggaaatctac cttgctacgc
     4321 aagaagtgga gcatattcga ctatttcaga tacaccagaa tgccaatctt acttgtccag
     4381 ttgggcagca tatagaaggt gcaatggttc ccatcttttc cagcgttgaa caagatctgg
     4441 aaaatcaact cagtcgtcaa tccttagata atgtgattga taatctctat caatccgcac
     4501 aagaaacccg aatctgacct cggtcagatt ttattaactc aaacatagac ctgtaattga
     4561 tacaggaaca aaccaaagaa tagaaaagag taaaccatga aagcagcaca acacacatct
     4621 tataataaaa acaagatcac ccttaacctt acagaaattg ctaaaccaca aatcaaacct
     4681 aaccaagtcc ttgtcaaagt gaccgcagca ggcgttaacc cattggacaa catgatttct
     4741 cgcggagatg tgaaattgat tgttccttat aaattgccac aaacggctgg aaatgaacta
     4801 gttggcttga ttgaagaagt aggttcaagc gtgacgacat ttgtagcagg tgatcgtgtc
     4861 ttcggccgtc ttccacttga tagtatcggt gcttttgctg agtatgtagc ggttgacgct
     4921 caggctcttg ctaaggttcc tgcctacttg acagatgaag aagcggctgc tgttcctttg
     4981 acagccttga ccatcatgca ggcccttgac ctcatgggtg ctgaggcagg caagaccatc
     5041 ttcatctcag gcggaactgg tggtgtcggc ggtatggcta ttccgattgc caaagccaag
     5101 ggcttgaccg ttatcacaaa tggtgacggt gcaagtggtg aacgtgtctt gaaactcggt
     5161 gcagaccggt ttatcgatta caagacagaa gattacacca aaacacttag caatgttgac
     5221 tatgtcttgg acacactcgg tggtgcagaa acggagaaac aaatgtccat catggaaaaa
     5281 ggtggacagc tggtttccct tcgtgccatg ccaaatggtg aatttgccaa acgtatgaat
     5341 ttgccaaaat ggaaacaaat cttgtttggc ctagctggtc gttcatttga caaaatggct
     5401 aagaaatatg gtgttcacta ccatttcatt ttcgtagaga gcaatggtcg ccagctgcaa
     5461 gaagtagcag acattttcag caagctcgaa attaaaccgt caatcgatac cgtttatcca
     5521 tttgaagaag tcaacgcagc actggataag gttgccaacg gtcgttcacg cggaaaaacc
     5581 gtcctcagtt tcaactaaaa gataaaatat acagggaaac atcatgtcat atattacaac
     5641 taaaaaccaa tacatcaccg tttcaaacaa taaaattgcc taccgtgagc tcagcaaggg
     5701 caagtctgat attcctttgg tcatgctggt tcacctagcc gcgaccttgg acaactggga
     5761 tccaaaatta ctggatttgc tggctgaaca gcaccatgta attgtcctag accttcctgg
     5821 cgtaggagct agtcaaggca aggtggctcc gactattcca ggaatggctg agcaggctgt
     5881 ttccattatc aaggctttgg gttatgagaa aatcaatttg ctgggtctgt ctatgggtgg
     5941 tttcattgct caagaaatcg ttcgtctgga tagtcagctg gtcaaccgtt tgattttagc
     6001 aggcacaggt cctcgtggtg gttttgaagt cgataaggtg actggaaaaa ccttccgcta
     6061 catgctgaag gctggtttgg agcgtgtgga tccaaaacgc tacatcttct acaatcatga
     6121 tgaggcaggt cgtttggagg cagaaaaagt cttgggtcgt atggggcaaa gaagtgctgc
     6181 tcatgcagac aaggacatga atgttccagg atttttgacc cagctgaaag ctatcaaacg
     6241 ctggggaaga gagccgcaag atgatatgac ctttatcacc caaccaactc tgatcgtcaa
     6301 cggtgataag gatatgcagg ttccaactga aaattcctat accatgcatg agaagatcaa
     6361 aaatagccaa ctgatcatct atccaaatgc aggacacggc tctatcttcc aaaatgtaga
     6421 ggatttttcg aaagccttac tagccttttt ggaggaaacc aatgcctaaa accattttaa
     6481 tcactggttc gacggatgga atcggcaaac acctagccat gaaactagct agtgaaggcc
     6541 atgaagtgat cttgcacgga cgaaatagcg aaaagttccg tgtagccctc agtgatattc
     6601 aactaaaaac aggaaataaa aagattcacg gctatcttgc agatttttcc aagttggcag
     6661 atgtctatcg tttcagccaa gaaattaaac gcgattttga gcagattgat gtcttggtaa
     6721 acaatgcagg agcttacttt ggtgatgctc gcgtggcaac ggcagaaaat atcgagatga
     6781 cctttatgct gtctgtccaa gttccctata tcttgacgac ggagttgctg cctttgctgg
     6841 aaaaagcagt aggtagggtc attcatacct cttccttcat gcaccatttt gcacaaacca
     6901 gaggcttgga ttttggttta gagcagagct attctgcagc tatggcttac aataatgcca
     6961 agctatacac catttggctg gccattgctc aggcagaggc cttagaaaag caaggttcat
     7021 ctgtgactgt caatgcctac catcctggct tgattgcgac aaatttgggc aatgatgggg
     7081 tcaaacgcaa cctgagaagt cggattctta ccagtctgat gaaaccattt tccaaggatt
     7141 tggaccaggg gattgaaaca ggttattact taagtttgtc accagaggta gctgggcagt
     7201 caggtcgcta ttttgcagag aaaaaagtgg caaaggtcag cctaaaaggc tttgatgtgc
     7261 caaagagcca agccttacta gcctatcttg ataagaaaat ccaagtattc aaggagagct
     7321 accgtgacta agattttgtt agaaaacctc caactgccca atggtcaggt tttagacaat
     7381 cgctttttca aatccgccat gagcgaaacc atggcagata gtcagggagc tccctcagat
     7441 gacctgattg ccttatatga tttttgggcc aagcagggaa tgggtgtcct agtgacaggg
     7501 aatatcatgg tggacggccg ctaccttggt gagccgggca atgtggtcct tgattcagac
     7561 gatcatctgg ctcaatttcg gaaatgggca gccgttggga aaaagaacgg tgtgcctatc
     7621 tggctccagc tcaatcaccc tggcaaacag atgtatcggt cgatcaatca aactccgatt
     7681 gcacccagtg ctattccaat ttctggaggt tcagcttcgg cctttcgccc accgagagaa
     7741 atgaccgtgt ttgacatcaa agaagctaga gataaattta ttgcagcagg ccttcgggca
     7801 aaagaagcag gatttacagg tgtacagtta catgctgcac atggttactt gatcaatcaa
     7861 ttcctctcac cagcagataa tcagcggacg gaccgctatg gtggcagtct ggacaatcgc
     7921 atgcgattct tggtggaagt ctatcaaggt ctgcgtgaca aagtgggacc agattttacc
     7981 atcgccttga aattgaatgc ttctgatttt aaggaagaag gctttggttt tgaagattgt
     8041 aagcatgtgg tgaaaaccat gtctgacttg ggtatcgacc tgattgaaat ttcaggtggc
     8101 aattatgaga cacctgtttt tggcagtgag tatgagaatg gtgctggatt tgtcacctac
     8161 gccctagcct tagcggattt gacttctgtt cctattgttt caacaggcgg tttccgaaaa
     8221 gtcactcaga tggaggaggc tatcaaggat ggtgtgtcca tgattggcct tgctagaccc
     8281 tttgtccttc gtccaagtct tgtcaatgat taccgtcaag tcggtgatgt gcaactgaca
     8341 acgcctcgcc tgacaacagg tcttccaaaa ctagataagg ctcttggtcc cattatcggt
     8401 gttagttatt acgaggccca gatgaaacgc ttggctatgg gaaaaagtgt caccgtcagt
     8461 caacatgcct ggccctatct tctccaaacc atcaaggcac acggtctgtc agccttgaaa
     8521 ccgaggagga agtagaatga tgctaatcat gacgatacta aattaatttt aaagctactt
     8581 ttacaatgcg tgtatgagta gctttatttt cattatagat attcgattga ggagaggttc
     8641 aagcctaacg ccgcttctca agatttgtat tacagtactt gaaatcatta ttaaaaatat
     8701 ccagagagat gatattatgc acaaaaaaga cttagagtga ctgaaaaaca gttgcctaag
     8761 tctttttgct tggtctggtc aagaacattg acaagcttgc tctattgctc acccatcaac
     8821 cgcatgatgt catcagggct ttccttacag cctctagcaa cccaggtttt aagcactcgg
     8881 aaaaatcctc ctacgataaa ttgcaggtga aaatcatcag tcaagtcccc cagcctttct
     8941 ttgaaatagc gatccaagac ctcctcgagc aagtagtcca aatggttggt agtcaataag
     9001 cgaatcttat cctgctgctc ataccaaaag tcaaaaaatt gccgtaaagc tagtaagaga
     9061 tgggcatctg accaatccga caagtcaatc tgccgctctg acaaccaggc ttgtaactcc
     9121 atgttgaagg accgttcgag cacctcttcc ttattggaaa agtttcggta aaaggtcacc
     9181 cgaccgatgc cagctcgctc taccaactca gatacactga tcttttcaaa agatttgtct
     9241 tccagcaact cccacaaagc ctccacgaca ctctccacaa ataacgattc tttccgcttc
     9301 actctttcca tgcgaaacac ttcctttcat tatgtttcct tttgggcaaa tagcagagct
     9361 gggactagtt tttccactgt ccatctgcta tactgataaa aaacgaaaca cttgtttcta
     9421 aaaaaatacc ataaaggaca gaaaaaagaa agtatgaaaa aagtttggaa aatagtagca
     9481 gctgtaacag ttgttctagc cttgcttggt tttattgcct acaaaaagac atttggctgg
     9541 tcagctcctg agcttgttgc tcagacccca caagtgaatg aatggtaccg cctcagtccc
     9601 gaaggggttg tagattctca gggcaatcag gcacacggac ttattcggac tggtaaagag
     9661 aagaataagg tgatggtcta tttcttcggt ggcggggtga gtattaatga agagactgct
     9721 agtggtggca cacgttattt tgcgaccaca actggccatc aagattatgt tgcgacttgg
     9781 ggaattggaa gtccgcagga agacaatcca tttaaggact ggactatgat tgtgcttccg
     9841 tatgggacag gagattttca tgcagggacc cagaactttt catatgtaga cgataacgga
     9901 aaagagcaag tagtccatca ccaaggttat tccaatctga tgagtatttt agcagcagcc
     9961 aaaccgcacg ttggcaatcc cgatacactt ctagtgactg gattttcagc tggcggattt
    10021 gcgacctcac tgctagcgga tgatgtcatt tctcattttc caacagcgaa aaatgtcaca
    10081 gttgcagtgg acagttcctt tttgagacat gataactgga aaagcattgc ggaaaatgtc
    10141 tggaaaacac cgacttccat tagtgatcgc ctgcattcag acaatattgt tttagatagc
    10201 ttagtagccc tccatgagaa acgcggggat agcgttaaga ttttatttga catttcctat
    10261 cgagatggaa ttcttcagca atatcaagcc tatattgacc aagggaaact agcagatgca
    10321 acagaagaga gtagtgacca ctttcaaaaa gaacttaaaa aaatgattca agaactgcaa
    10381 tctaagattg atggcgtggg tatttttatc tggaattatg ggcaagatga aaaaactacc
    10441 gctactcagc acaccaccat taacttctca acattcttta cccctatgtc taaagacaag
    10501 agtgtggcag aatggctgga tgatgctgtc aatggacagg taaacagtta tggattagaa
    10561 ctattagaat aggagaacaa catgaaaaaa tttaaaaaaa tcatcttatg gacccttggg
    10621 attgtagcta gtctcggtat cattgcttat gtgtctgtta ccaatcatcc gcagttggta
    10681 gtcggtgtca ttcaatcgtt tatgtacaag gagagttcgc cgaactctta tagtcctctt
    10741 tatgatccca ttgatggttt gaaagagaat ggtcagtatt taaaaagtga gattgcctat
    10801 agcaaggact accctaacag ctttttagat attacctatc cagaccaaaa tcttgaagct
    10861 gaccgtccaa ccctcttcta tttccacggt ggtggattct ttggaggcag caagaatatg
    10921 ggggaccctc tggctgccag ccaagcgacc tatctcattg acgatatcgt ttcaaaaggt
    10981 tacaacgttg tcaatgttga ctatgccctt gttccagact atcattttcc aacgcctgtg
    11041 attcagatga atcaggccat tgcctacatg aaggaacacg ctgaagaata ccatttgaac
    11101 atggataatg tcgtcttgat gggctcatca gcaggtgcca tcatggtagc acaatacggt
    11161 agcctcttag ccaacaatga ttacgctcag ctattgaaca ttgaaccaag tattgcaaaa
    11221 gaacatgtca aagctctggt tatcgacgac gctcccttag attacgccaa gtttagctta
    11281 agcacgaagc tcttggttgg aaattatgtt tcagaaacca tttatctaag tgaggaagaa
    11341 atcaatgcct ataacccgat ttcccatgtg aacaaggact acccagctag tttccttctg
    11401 ggaagcgagt atcggacaga tatggtgtcg ctcaataagg ccttgaagca agcgggagtt
    11461 gaaaatgaat tggtcgatcc tttagcagaa gaggggcttg aaaagcctca tggttttgtg
    11521 gcgaatttac gaactgaccc tgtgtctgcc aaagcatttg aacggttgat tacttttata
    11581 gacgatagaa ccaaggagta gtaggagagt ggataagctg gaaactgatg gggatttgtc
    11641 caatcaatct gccgctctga caaccatgtt tgtactcaga aagtctacgc cgaaaaattc
    11701 caaatgagaa caataagtcc cagctctgcc atttcccaaa agtacgttat aaaatcgttg
    11761 aaatccttgt caattgtgtt ataatgaaga gtatgcaata aaattttaag gagaatgaca
    11821 gaatgtctgt atcatttgaa gcaaaagaaa caaaccgcgg cgttttgact ttcacaatcg
    11881 gtcaagatgc tatcaaacca gaattggacc gcgttttcaa caaagttaag aaagatatca
    11941 atcttccagg tttccgtaaa ggtcacttgc cacgtgccgt tttcaaccaa aaatttggtg
    12001 aagaagcact ttatcaagat gttgtaaatg cattgttacc agcagcttat gaagcagcgg
    12061 ttgcagaagc tggtcttgaa gtagttgcac aaccaaaaat tgacgttgtg tctatggaaa
    12121 aaggtcaaga ctggacaatc actgcggaag ttgtgacaaa acctgaagta aaattgggtg
    12181 actacaaaaa cttggcagtt tcagtagaag ctactaaaga agtaacagat gaagaagttg
    12241 atgcaaaaat cgaagctgca cgcaacaact tggctgaatt ggtcatcaaa gaaggaccag
    12301 cagctgaagg cgatacagtt gttatcgact ttgtaggttc aatcgacggc gttgaatttg
    12361 acggcggtaa aggtgagaac ttctcacttg gacttggttc aggtcaattc atcccaggtt
    12421 tcgaagcgca attggtaggt cacgcagctg gtgaagaagt aaacgttgaa gtgactttcc
    12481 cagaagacta ccaagcagct gaccttgctg gtaaaccagc cctcttcgtg acaaaaatcc
    12541 acgaagtaaa agcaaaagaa gttccagctt tggatgacga attggcaaaa gacatcgacg
    12601 aagaagtaga aacacttgat gagttgaaag ctaaataccg caaagaattg gaagcagcaa
    12661 aagaagttgc ctttgacgat gcagttgaat cagcagctct tgaattggct gtagaaaacg
    12721 ctgaaatcgt tgaattgcca gaagaaatga ttcacgaaga agttcaccgt gcaatcaatg
    12781 aattcttggg tggtatgcaa caacaaggta tctcaccaga tatgtacttc caaatcactg
    12841 gtacaactcg tgaagacctt cataaacaat acgaagcaga tgctgaaaaa cgtactaaga
    12901 caaacttggt tgttgaagca gtggctaaag cagaaggttt cgaagcaact gaagaagaaa
    12961 tcaacaaaga aatcgaagac ttggcagcaa cttataatat ggaagtggca caagtacgta
    13021 gcttgctttc accagaaatg cttaaacacg acatcgctgt gaagaaagct gtagaagtaa
    13081 ttacaagcac tgcaactgta aaataattat ttggaaaact cgactctagt cgggtttttt
    13141 gtttaatcat ctttcgatgt tacaaagaaa ctctctgaaa aaccttgtcc gtcccatgca
    13201 gaaatgggag aatgtcatat ctgttggtgg agtcgtccaa gtttcacaag gggtacgaat
    13261 aactgacgga gaggtcagtc attaccagtt gccgtggtcg gtgctcctat gtttacaagc
    13321 tatcgaatat gactgttctt ttttagggag tagaatttta ttggactatt tgcaatccaa
    13381 tctcatcata tcaggtgcct ttgggttatt ttacaaggag tatgtcatag cagttggggg
    13441 ttatgatagg cagacactgg gagaagatat ggagctagtc atgaaattgc attatttctg
    13501 taaaaataac aatattcctt atcgaatctg ctatgaaacc agcgccattt gttggagtca
    13561 agcaccgagc tctatcggtg atttatgcaa acaacgccgt cgctggttct tgggactcta
    13621 tcaatgtcta aaaaaatacc gaaaaatgtt atcgcgagtt cgttttgggg cggtgggata
    13681 gttttcctat gcttactata tgctatttga atttttggct ccgtttatag agactttcgg
    13741 ttgtttcgtg attctactgt cgttgatcta tcaccaacta aacatctcat ttttcatatc
    13801 gctctttttc ctctatagtg ctttttgctc actaatcacc tttgtgtcat ttttgcaacg
    13861 cgtctactcg caagatttat ttattggacc tatggatttg tgtaaagcct tcctggcaac
    13921 cttgtttaga tattttttct tgcattgggt attgaatatt gtccgtctga caagttttat
    13981 tggatataaa aagagaaagc gtgaatgggg agaaatcaaa cgagtgaaac aattcgttt
//
LOCUS       DAZQCJ010000048        13944 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000048 DAZQCJ010000000
VERSION     DAZQCJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13944
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.049"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..229)
                     /locus_tag="ACHU66_001268"
     CDS             complement(<1..229)
                     /locus_tag="ACHU66_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389554.1"
                     /translation="MRRLVLNIAMSLDGFIARKDGSYDWIDGHGTDKYDTVSQFDNQK
                     FFRGWAKRPTSLLRVRVNISAQWLIGRFVRVL"
     gene            complement(373..1326)
                     /locus_tag="ACHU66_001269"
     CDS             complement(373..1326)
                     /locus_tag="ACHU66_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR3389555.1"
                     /translation="MNVSILALLISQMLIYSAPLIFTSLGGVFSERGGIVNVGLEGIM
                     VIGAFAGVVFNIEFAGTFGNATPLLAIIVGGLAGVVFAAIHAMATINFRADHVVSGTV
                     LNLLAPALGVFLVKVIYNKGQTDSITESFGKFSFPILADIPIIGEIFFKNTSLMGYVA
                     IATAFLAWFILYKTKFGLRLRSVGEHPQAADTLGINVYAMRYAGVLIAGFLGGVGGAV
                     SAQSVNINFSATTIVGSGFIALAAVIFGKWNPIGAMLASLFFGLSQSLAVVGGQLPGL
                     KDIPTVYLQIAPYLITVIALSAFFGKAVAPKADGINYIKSK"
     gene            complement(1328..2389)
                     /locus_tag="ACHU66_001270"
     CDS             complement(1328..2389)
                     /locus_tag="ACHU66_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR3389556.1"
                     /translation="MLNKYKNLALPLMAVVSGILLGAIVMLVFGYDPIWGYEELFYSA
                     FGNIKSIGEIFRAMAPLVFTALGFAVASRAGFFNVGLSGQAYVGWVFAGWFALANPDL
                     PRPVLILGTVLIAMLAGGIAGAIPGILRAYLGTSEVIVTIMMNYILLYSCNYIIRDVF
                     ADSLMKNTDSTVNVSANASYQTEWLRALTDNSRMNIGIFFAIIAVFVIWFLLTKTTLG
                     FEIRSVGLNPTASDYAGMSAKRTIILSMIISGALAGLGGAIQGLGTFQNVYIQSGNLD
                     IGFNGMSVALLASNSPLGIPLAAFLFGVLSVGAPGMVRAQIPPELINVVTASIIFFIG
                     VKFIFEQLLKSRNKAKGAK"
     gene            complement(2382..3917)
                     /locus_tag="ACHU66_001271"
     CDS             complement(2382..3917)
                     /locus_tag="ACHU66_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3389557.1"
                     /translation="MTKDYVIEMRNITKIFGEFVANDHINLNVRRGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIVINGQSVKIDSPSKAAQLGIGMVHQHFMLVDAFTVAENI
                     ILGSETTKGGVIDLKKAIAEIKELSAKYGLEVDPTAKVADISVGAQQRVEILKTLYRG
                     ADLLIFDEPTAVLTPAEIAELLKIMKALVEEGKSIILITHKLDEIRAVADSVTVIRRG
                     KSIETVAVEGATNQDLAEWMVGRSVSFKTEKVEASPKEVILSIKDLVVNENRGIPAVK
                     GLSLDVRAGEVVGIAGIDGNGQSELIQAITGLRKVKSGSITIKGEEVVGKTPRKITEM
                     QVSHVPEDRHRDGLVLPMTVAENFALQTYYKEPISKNGILNYNIINEKARALMEEFDV
                     RGASELVPIKALSGGNQQKAIIAREIDRNPDLFIVSQPTRGLDVGAIEYIRKRLIAER
                     DKGKAVLVVSFELDEILDVSDRIAVIHDGVIHGIVEPAKTNKQELGVLMAGGHIEKGE
                     EHA"
     gene            complement(4112..5179)
                     /locus_tag="ACHU66_001272"
     CDS             complement(4112..5179)
                     /locus_tag="ACHU66_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HFR3389558.1"
                     /translation="MNKKLVGLGLAAVAAVSLAACGNRASKSSSSEASSSADTSVKAA
                     IVTDIGGVDDRSFNQSAWEGLQEWGKAASLSKGNGYDYFQSASESDYITNLDSAVAGG
                     YNLVFGIGFALESAIAEVAPNNPNTHYVIVDSVVPDQDNVVSVGFADHEASYLAGVAA
                     AKSTKTNHVGFIGGMEGVIIDRFEAGFVAGAKSVNKDIKITVDYAGSFGDAAKGQTLA
                     AAQYAAGADVIFHASGGTGNGVFAAAKAENETRNEADKVWVIGVDRDQSAEGTYTSKD
                     GKSSNFVLASTLKQVGTSVKDIATKAAAGDFPGGQVLQFSLKDKGVELAETNLSEDAS
                     KAVADAKQAILDGKVEVPEKP"
     gene            complement(5260..5649)
                     /locus_tag="ACHU66_001273"
     CDS             complement(5260..5649)
                     /locus_tag="ACHU66_001273"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR3389559.1"
                     /translation="METIDLIELAVKNSQKAYVPYSKFPVSAVLVAETGEIFTGVNIE
                     NASFGLTNCAERTAIFKAISEGVKDFSEIVIYGKTEKPISPCGACRQVMAEFFDKDLK
                     VTLVAKDKSTVETTVGELLPYSFTDLT"
     gene            complement(5636..6298)
                     /gene="deoC"
                     /locus_tag="ACHU66_001274"
     CDS             complement(5636..6298)
                     /gene="deoC"
                     /locus_tag="ACHU66_001274"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR3389560.1"
                     /translation="MKLNKYIDHTILKPETTQEQVEKILAEAKEYDFASVCVNPTWVA
                     LAAESLKDSDVKVCTVIGFPLGANTPAVKAFETKDAISNGADEIDMVINIGALKTGNY
                     DLVLEDIKAVVAASGDKLVKVIIEACLLTDDEKVKACQLSQEAGADYVKTSTGFSTGG
                     ATVADVALMRKTVGPDMGVKASGGARSYEDAIAFIEAGASRIGASSGVAIMNGAQADG
                     DY"
     gene            complement(6314..7591)
                     /locus_tag="ACHU66_001275"
     CDS             complement(6314..7591)
                     /locus_tag="ACHU66_001275"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFR3389561.1"
                     /translation="MRAVDLIQKKRDGLELSSEEIKWLIDGYVAGTVPDYQMSALAMA
                     IYFKGMSTREISDLTMSMVVTGEQIDLSAIPGIKVDKHSTGGVGDKVTLILAPLVASF
                     DIPVAKMSGRGLGHTGGTLDKLESIKGYQIEVSQADFIKQVQDTGVAVIGQSDNLVKA
                     DKLLYALRDVTATVDIIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVEEARVLA
                     QTMVDLGKAVGRKTVAVLTDMSQPVGTSIGNRLEILEAIEILQGKGREDVTEFICELA
                     QIMLGLANVEKSIEEVRQHIDNGAALKKFEEMVRAQGGDLEDLYRPVQVTQQVPVLAE
                     EDGYIVGLPALEFGLFAMKLGAGRAVKTDNLDYETGIVFHKKVGEAVSKGEQIATIYA
                     NENISENMLTNFQKNVKIDKESVKTKEIIEIIS"
     gene            complement(7588..8178)
                     /locus_tag="ACHU66_001276"
     CDS             complement(7588..8178)
                     /locus_tag="ACHU66_001276"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR3389562.1"
                     /translation="MSNMYYEKNPTIAHDIHEIHVNLLDTPMSFMTDAGVFSKKMVDY
                     GSQVLLKTLHFEKGASVLDVGCGYGPIGLTLAKVFNTKTTLIDINSRALDLATKNAER
                     NGVIAKIYQSNIYENVDETFNHIVSNPPIRAGKSVVHEVIAGAFQRLEAHGTLSIVIQ
                     KKQGAPSAKAKMEEVFGNCQIIKKDKGYYILESVKE"
     gene            8287..9207
                     /gene="coaA"
                     /locus_tag="ACHU66_001277"
     CDS             8287..9207
                     /gene="coaA"
                     /locus_tag="ACHU66_001277"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009568566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR3389563.1"
                     /translation="MKNEFLNFEQIDRATWQQLHRKTTIPLSQSELNSIKSFNDRIQL
                     HEVSDIYLPLVNLIHIYRKARKDLNFTKSLFLQKTIKPQPFIIGVSGSVAVGKSTTSR
                     LLQILIARTFKHAKVELVTTDGFLHPNAVLEERQLLNKKGFPESYDMEKLIDFLDKIK
                     NGYDCQIPVYSHEIYDIVPNKTQEIKSPDFLIVEGINVFQNPQNQRLYVSDYFDLSIY
                     VDADVEHIETWYLERFQKLLTLAKNDPNNYYHRFTQMTYPEILSIAQNTWKNINLANL
                     EKFIEPTRNRADIILHKAENHEIDKIYLKK"
     gene            9266..9514
                     /gene="rpsT"
                     /locus_tag="ACHU66_001278"
     CDS             9266..9514
                     /gene="rpsT"
                     /locus_tag="ACHU66_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR3389564.1"
                     /translation="MEVKPLANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANP
                     SEELFRAASSAIDKAETKGLIHKNKASRDKARLASKLA"
     gene            complement(9776..11005)
                     /locus_tag="ACHU66_001279"
     CDS             complement(9776..11005)
                     /locus_tag="ACHU66_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFR3389565.1"
                     /translation="MRKVSRGVLTILLSATVLSACSGGGQELSERLEAAKIEFSKEKK
                     QLEAELTALKEEVQGNFYYQQLDTDKERRVYLQFVNGLSKRMQVIDIDSVNDEIYSRV
                     YFSVAHDYPEFYWLTDEAAMAGGIDILDLEEPVYPSDLSPTQNKIEDEVNKILAQTPK
                     GSDYEKVKYFYEVIIKQTDYDLEALQTKSVTWRSQGITSVLLDKKSVCAGYSRTFQYL
                     CKKAGIDCIYVTGIAKNGQNGEFGHAWNLVKINGQYYGVDTTWGDPVFDQAISGEAHT
                     DISYDYLCVPDEILERSRIADSDLLDYWGEEQYYEPRVLVYPKCTDNSLNYYVQKGVY
                     FTSFDEAAVLQSITDQRLQGTNKVVLQFGTAEAMQQMITLASTENNAIFQALGDVGEY
                     QYYYNDQTYTFELADWF"
     gene            complement(11329..12708)
                     /locus_tag="ACHU66_001280"
     CDS             complement(11329..12708)
                     /locus_tag="ACHU66_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFR3389566.1"
                     /translation="MPKRFKKLMYTDKFSFFIRYFAVFTLIFGLMTAIIFQLMRSTMY
                     QNSDNTLKRIKKEPALAVGFAIARTYEPNSVFILQDSPTGEETTSSSSDSTPVPKNQK
                     NTRAGDQLRLGANTHVLLYSKSGEMINPDTFTGLADLSLDKEKLGEIKETTVESSFGM
                     SEDYRYITIGLATDELGYYSSYDIKYATILVNVSQIKSSIESYESTVAIVMVSAWLIS
                     ILASIYLSNLSMRPILISYQKQKDFVENASHELRTPLAVLQNRLESLFRHPETTILES
                     SESIGSSLEEVRNMRLLTTNLLNLARRDDGLKVDMIDVQPNYFDEIFANYLMIAEENG
                     KTLTVNNLIHQPIRTDKVLIKQLLTILFDNAMKYTDDDGTIQMAANIKDKLVCFTVID
                     NGLGISDADKKKIFDRFYRVDKARTRQKGGFGLGLSLAQQIIKNLDGEISVRDNQPKG
                     TIFEVRLPK"
     gene            complement(12701..13372)
                     /locus_tag="ACHU66_001281"
     CDS             complement(12701..13372)
                     /locus_tag="ACHU66_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR3389567.1"
                     /translation="MIKILLVEDDLSLSNSVFDFLDDFADVMQVFDGEEGVYEAETGV
                     YDLILLDLMLPEKDGFQVLKELREKGVTTPVLITTAKESLEDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRAGKFNENTLTFGDVTVDLSTNMTMVNGEEVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRIWGFDSDTTISVVEVYVSKIRKKLKGTTFGENLQTLRSVGY
                     ILKNA"
     gene            complement(13566..>13944)
                     /locus_tag="ACHU66_001282"
     CDS             complement(13566..>13944)
                     /locus_tag="ACHU66_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389568.1"
                     /translation="SFINQFKSQSKTVLAAVAISAATLGFAGGMSISKSSTGVSTQSQ
                     ASLAQNSSAAMNGQGAPQGMPPGKGGPSQGMPPGQGTPPHDATSGATQSNSSTSSDEV
                     NALKKKNQEIKAQISGDSSSSNQ"
BASE COUNT         4323 a         3106 c         2338 g         4177 t
ORIGIN      
        1 gtaaaacacg aacaaatctg ccaatcaacc actgcgctga gatgttgaca cgaaccctga
       61 gaagcgaggt tggtcttttt gcccagcctc taaagaattt ttgattatca aactgtgaga
      121 ccgtatcgta tttatcggta ccatgtccgt caatccaatc gtagcttcca tccttacgtg
      181 caataaatcc atctaaactc attgcaatgt ttaacaccaa tcgtctcaca attagcctca
      241 tttcatacct tagtgacaag ctattctccc tattctcaac caaaagtccc tataactcct
      301 cttcctttga ataggacaat tattcatttc ctgtacagca aagaacatga taaaaaggtc
      361 cactggacct ttttatttag atttgatata gttgataccg tctgctttag gagcgactgc
      421 tttaccaaag aaagcagaca gagcaataac agtaatcaaa tatggtgcaa tttgaagata
      481 aaccgttgga atatctttca atcctggtaa ttgtccaccc actactgcca aactttgcga
      541 aagaccgaag aatagactgg caagcatagc gccaattgga ttccacttac caaaaattac
      601 agctgctaga gcgatgaaac ctgaacctac gattgttgtt gcagagaagt tgatattcac
      661 tgattgagca cttacagctc caccaacacc accaaggaaa ccagcaatca acacacctgc
      721 ataacgcata gcgtagacat tgattcccaa agtatccgct gcttgcggat gttcacctac
      781 tgaacgaagg cgcaaaccga acttggtttt ataaaggatg aaccaagcaa gaaatgctgt
      841 tgcaatagct acataaccca tcaagcttgt atttttgaag aaaatttcac caatgattgg
      901 aatatctgcc aagataggga atgaaaattt accaaatgat tcagtaatac tatcagtttg
      961 acccttatta tagataacct tgactaggaa aactcccaat gctggagcca aaaggttcaa
     1021 caccgtacct gatacaacgt ggtctgcacg gaaattaatc gtcgccatcg catgaatagc
     1081 tgcaaagaca acaccagcaa gacctcctac aataatagct aataatggag tagcattacc
     1141 aaatgtccct gcaaattcga tattgaaaac aacaccagca aaggcaccga taaccatgat
     1201 tccttctaga ccaacgttta caataccacc gcgttcagag aaaactcctc caagactggt
     1261 aaagattaag ggtgctgaat aaatcaacat ttgcgaaatg agtaaggcaa gaattgaaac
     1321 attcatctta tttcgctcct ttcgctttat ttctagattt taataattgt tcaaaaataa
     1381 atttcacacc gataaagaaa atgattgatg ccgtaacaac gttgatcaat tcaggtggaa
     1441 tctgtgcacg taccatacct ggggcaccga cagaaagaac accgaataga aaggcagcca
     1501 atggaatacc taacggtgaa ttagaagcta aaagcgctac tgacatacca ttaaaaccaa
     1561 tatccaagtt accgctttgg atataaacgt tttggaaagt acccaaacct tggatagctc
     1621 ccccaagacc cgcaagagca cctgaaataa tcatagatag aataatcgta cgtttcgcag
     1681 acatacccgc ataatccgat gctgttgggt taagccccac tgagcgaatc tcaaaaccaa
     1741 gagtcgtttt ggtcaatagg aaccaaatca caaaaactgc aataatggca aagaaaatac
     1801 caatattcat acgtgagtta tctgtcaatg cacggagcca ctctgtttgg taagaggcat
     1861 ttgctgacac gttaactgtc gagtctgtat tcttcatcaa actatcagca aatacatcac
     1921 gaatgatata gttacatgaa tagagtaaga tgtagttcat cataatcgta acgataactt
     1981 cacttgttcc caaataggca cgtagaatac ctggaatagc tccagcaata ccgcctgcca
     2041 acatagcaat caagactgtt cccaaaatca aaactggacg tggtaagtca ggattcgcca
     2101 aggcaaacca tccagcaaaa acccaaccaa cataggcctg accggacaaa ccaacgttga
     2161 agaaacctgc acgactagct actgcgaaac cgagagcagt gaaaacaagt ggtgccattg
     2221 cacggaaaat ttcaccaatt gatttgatat taccaaaagc tgaatagaat aactcttcat
     2281 agccccaaat tggatcgtag ccaaaaacaa gcatgacaat cgctccaagt aggataccac
     2341 tgaccacagc cattaatgga agggccaaat tcttatattt attaagcatg ttcttctccc
     2401 ttctcaatat gtccacctgc catcaagaca cctaactctt gtttattggt cttagcaggt
     2461 tcaacaatac catggatgac accatcatgg ataacagcaa ttctatctga tacatccaaa
     2521 atttcatcca attcgaagct gacaacaaga acagccttac ccttatcacg ttctgcaatg
     2581 agacgcttac gaatatactc aatagcacca acatccaaac cgcgtgttgg ttggctaaca
     2641 atgaaaaggt ctgggttgcg atcgatttca cgcgcaataa tagctttttg ctggttaccg
     2701 cctgaaagag ccttgattgg aacgagttca ctcgccccac gaacgtcaaa ttcttccatt
     2761 aacgcgcgtg ctttttcatt aatgatatta tagttcaaga taccattttt tgaaatcggt
     2821 tctttatagt aagtttgaag tgcaaagttc tcagcaacag tcatcggaag aacgagtcca
     2881 tcacgatgac gatcttcggg aacgtggctc acttgcattt ctgtaatttt acgtggagtt
     2941 ttaccaacta cttcctcacc tttgatagtg attgagccag attttacctt acgtaaacca
     3001 gtgattgcct gaatcaactc actttgaccg tttccgtcga taccggcaat accaacaacc
     3061 tcacctgctc gaacatcaag cgacaagccc ttaactgctg gaataccacg gttttcattg
     3121 acaaccaaat ccttaattga caaaatgact tctttaggac tcgcttcaac tttctctgtt
     3181 ttaaaggaaa cggaacgtcc aaccatccac tcagccaaat cttggtttgt tgctccttca
     3241 acggcaactg tttcgattga tttacctcga cgaataactg ttacactatc ggcaactgca
     3301 cgaatttcat ccaatttgtg cgtaattaaa ataatagatt taccttcttc aaccaaggct
     3361 ttcataattt tcaagagctc tgcaatttcc gcaggggtca atacagctgt cggctcatca
     3421 aaaattaaaa ggtcggctcc acggtaaagt gttttaagga tttctacacg ttgctgtgca
     3481 cccacagaaa tatctgcaac ctttgcagtt ggatcaactt caagaccata tttagcagaa
     3541 agttctttaa tttctgcgat agctttttta aggtcaatca cgccaccttt ggtggtttca
     3601 gaaccaagaa tgatgttctc agcaacagtg aaagcatcaa ccaacataaa gtgttggtgc
     3661 accataccga tacccaattg ggctgctttg gatggagagt caatcttcac ggattgaccg
     3721 ttgataacaa tttcaccgct tgtaggttcc aacaagccag caagcatgtt catcagagta
     3781 gatttacccg caccattctc accaagaagg gcatggattt caccacgtct gacattcaaa
     3841 ttgatgtggt catttgctac aaattcacca aatatcttag tgatattgcg catttcaatg
     3901 acataatcct tagtcataat ctattatcct ttctgactat agccttctga aaattcttca
     3961 gtactacttt gagtacttaa aaactcttca tcaactatac catttttgtc agtaaaacct
     4021 accatagctg ataggcttta ctgagaaaaa tctcagcccg aaaacggcgc cccgtcgggt
     4081 cgccgaatcg gaattgtcga aaagaaattc attaaggttt ttcaggaact tctactttgc
     4141 catccaaaat tgcttgttta gcatctgcaa ctgctttaga agcatcttct gaaagattag
     4201 tttctgccaa ttcaacacct ttatctttca atgagaattg aagtacttga ccgccaggga
     4261 aatcacctgc agctgcctta gttgcaatat ctttaaccga agtaccaact tgtttcaatg
     4321 ttgaagccaa tacaaagtta gaagacttac catctttaga agtgtatgta ccttctgctg
     4381 attggtcacg gtcaacaccg attacccaaa ctttatctgc ttcattacga gtttcgtttt
     4441 ctgctttagc agctgcaaat acaccgttac cagtaccacc agaagcgtgg aaaataacat
     4501 ctgcacctgc agcatattga gcagctgcaa gagtttgacc tttagcagca tcaccaaatg
     4561 aacctgcata gtcaactgta atcttgatgt ctttgtttac agacttagca ccagcaacga
     4621 aaccagcttc aaagcgatcg atgataacac cttccatacc accgatgaaa cctacgtgat
     4681 ttgttttagt tgatttagct gcagcaacac cagcaagata tgatgcttca tgatctgcga
     4741 aacctacgct aactacattg tcttgatctg gaacaacact atccacgatt acatagtgtg
     4801 tattaggatt atttggtgca acctcagcaa tcgcactctc caaggcaaaa ccaataccga
     4861 acacaagatt gtaaccacca gcaacagctg aatcaaggtt tgtgatataa tctgattcac
     4921 tagctgattg gaagtaatcg tagccattac ctttggaaag actagctgct ttaccccact
     4981 cttgaagacc ttcccaagca gattggttaa atgaacggtc atcaacacca ccaatgtcag
     5041 taacgatagc agctttcaca gatgtgtccg ctgatgaaga tgcttctgag ctactgcttt
     5101 ttgaagcacg gttaccacat gcagccaaag atacagccgc aacagctgct agaccaagtc
     5161 caacaagttt cttgttcatt tctgaaccct cctaaaaatc tttttgcaac taaattgttg
     5221 cagagtaata ttgctacagt caaatgactg tcatacagac tatgttaagt ctgtaaagga
     5281 atatggtagt aattccccga ctgtcgtctc gactgtcgat ttatctttag cgaccaaggt
     5341 cacttttaaa tccttatcaa aaaattctgc cataacttgg cgacaagctc cacaaggaga
     5401 tattggtttt tctgttttac cataaattac gatttcagaa aaatctttca caccttcaga
     5461 aatagccttg aaaatagctg tcctttcagc gcaattggtc aaaccaaaac ttgcattttc
     5521 aatgtttaca cctgtaaata tttctccagt ctcagctaca agaactgcac taactggaaa
     5581 ctttgagtac ggtacgtatg ctttttgact gttttttaca gctaattcaa ttaaatcaat
     5641 agtctccatc cgcctgtgct ccattcatga ttgccacgcc tgatgacgca ccaatacgag
     5701 acgctcctgc ttcgataaag gcaatggcat cttcatacga acgtgcacca cctgaagcct
     5761 tcactcccat gtcaggaccc actgtttttc tcattaaggc aacatccgct actgtggcac
     5821 cgcctgtaga aaatcctgta gatgttttaa catagtccgc accagcttct tgagataatt
     5881 gacaagcttt cactttctca tcatcagtaa gaaggcatgc ttcgatgata actttgacta
     5941 gtttatcacc acttgctgct acaacagctt taatatcctc caaaaccaaa tcgtaatttc
     6001 cagttttcag agcaccgata ttgataacca tgtcaatctc atctgctcca tttgaaattg
     6061 catcttttgt ttcgaacgct tttacagcag gtgtgtttgc tccgagaggg aaaccaataa
     6121 ccgtacaaac ttttacatca ctatctttca agctttcagc tgctaaagca acccaagttg
     6181 ggttaacaca gacacttgca aaatcatatt cttttgcttc tgctaaaatt ttttcaacct
     6241 gctcttgtgt tgtctcaggt ttcaagattg tgtggtcaat atatttattc aatttcatag
     6301 ctctctccgt acattaagaa ataatctcga tgatttcttt cgtttttaca ctttctttat
     6361 ctattttaac atttttttgg aaatttgtaa gcatgttttc cgaaatattt tcattggcat
     6421 aaatcgttgc gatttgttcg cctttagaaa cagcttcccc cactttctta tggaaaacaa
     6481 tacctgtttc ataatctaaa ttgtcagtct tgactgcacg acctgcacca agtttcattg
     6541 caaagagacc aaattccaaa gcaggaagtc ccacaatata gccatcctct tccgcaagaa
     6601 ctggaacttg ctgtgtaacc tgaactggac gatataaatc ttctaagtca ccaccttgag
     6661 cacggaccat ctcttcaaat ttcttcaatg ctgcaccatt gtcaatatgt tgacgtactt
     6721 cttcaatgct tttctcaaca ttagcaagcc ccaacataat ttgggctagt tcacagataa
     6781 attcggtaac atcttctcga cctttaccct gtaaaatttc aatagcctct aaaatttcca
     6841 aacgatttcc aatcgaagtt ccaactggtt gagacatgtc agttaaaaca gcaactgtct
     6901 tacgaccaac cgctttccca agatccacca tagtttgagc tagaacccgt gcttcttcaa
     6961 cagtcttcat gaaggcacct tctcccaccg tgacatctag aagaatcgca tccgcacctg
     7021 cggcaatttt cttagacatt actgagctgg caatcagcgg aataatgtca acggttgctg
     7081 tcacgtcacg aagggcatag agtagtttat cagctttgac aagattatct gactgaccaa
     7141 taactgcaac accagtatct tgaacttgct tgatgaaatc tgcttgacta acttcaatct
     7201 ggtatccttt gattgactct aacttatcca aggttccacc agtatgacca agcccacgac
     7261 cgctcatctt tgcaactgga atatcaaaac ttgctaccaa tggcgctaaa atgagtgtaa
     7321 ccttatctcc gacaccacct gtagaatgct tatctacctt gatgcctgga attgcagaca
     7381 aatcaatctg ttcacccgtt acaaccatgg acatcgtcaa atcagaaatc tcacgcgttg
     7441 acatcccttt aaaataaatc gccatcgcta aggctgacat ttgataatcc ggtactgtcc
     7501 cagctacata tccatcaatc aaccatttga tttcttcaga cgatagttct aagccatcac
     7561 gctttttttg aattaaatca actgctctca ttctttcaca ctttcaagga tgtagtaacc
     7621 cttatctttc tttataatct ggcagttgcc gaatacctct tccatcttag ctttagcact
     7681 cggtgcgcct tgtttctttt gaatgacaat ggacaaggtt ccgtgtgcct ctaagcgttg
     7741 aaaggcccca gcaattactt catgtacaac cgattttccg gcccgaatag gtggatttga
     7801 aacaatatga ttgaatgttt cgtccacgtt ttcgtagata ttggattgat aaattttagc
     7861 gataacacca ttcctctctg cattttttgt agctaaatcc aaagctctgc tattaatatc
     7921 aatcagtgtt gtcttggtgt taaacacctt agcaagtgtc aaaccaattg gtccataacc
     7981 acaaccaaca tccaacaccg atgccccttt ttcaaaatga agggttttta aaagcacttg
     8041 actaccataa tcaaccatct tcttgctaaa aacacctgca tctgtcatga aagacattgg
     8101 ggtgtctagt aagtttacgt ggatttcatg aatatcatgt gctattgtag gatttttttc
     8161 gtaatacata ttggacatgt acttattata acatttccta aaaacgtttt caagagtttt
     8221 tcttacatga aaaatatgat aaaatgaaat cgatattgaa acgcttacct ataggaagaa
     8281 ttatgtatga aaaatgaatt tttaaatttt gaacaaattg accgagccac ttggcaacaa
     8341 ctacatcgta agactaccat tccacttagt caaagcgaac taaattctat taaaagtttt
     8401 aacgaccgca tccaattaca tgaggtttct gacatctatc ttcctttggt taatcttatc
     8461 catatctatc ggaaagcacg caaagaccta aattttacga agagtttatt tctacagaag
     8521 actatcaagc cacaaccatt tatcattggc gtttctggaa gtgttgctgt cggcaaatca
     8581 acaactagta gattgctgca aattcttatc gctcggacat ttaaacacgc caaggttgaa
     8641 ttagtaacaa cagatggttt tctccatcca aatgcggtct tagaagaacg gcaactgtta
     8701 aataaaaaag gttttcctga atcctatgac atggagaaat tgattgattt ccttgataag
     8761 ataaaaaacg gctacgactg ccaaattcct gtctattcac atgaaatcta tgatattgtc
     8821 cccaacaaaa ctcaggaaat taaatctcca gatttcttga ttgttgaagg aatcaatgta
     8881 tttcaaaatc cacaaaacca gcgcctctat gtcagtgact attttgacct atctatatac
     8941 gttgacgctg atgtggagca tattgaaacc tggtacttgg agcgattcca aaaattattg
     9001 acattggcaa aaaatgaccc taacaactat taccatcgtt ttacccagat gacgtaccct
     9061 gagattctat ctattgccca gaatacttgg aaaaatatta atctagctaa cttagaaaaa
     9121 ttcattgaac caaccagaaa ccgtgctgat attattcttc ataaggccga aaaccatgag
     9181 attgataaaa tttatctaaa aaaataaatt tcacttgtca aaaatactct tttcagatat
     9241 aatggtatag ttagtacaag aaagggtgga ggtgaaacca ttggcaaaca ttaagtcagc
     9301 tatcaaacgc gctgaattga acgttaaaca aaacgagaaa aactcagcac aaaaatcagc
     9361 tatgcgtact gcaatcaaag catttgaagc taacccatct gaagagcttt tccgtgctgc
     9421 tagttcagca atcgataaag cagaaactaa aggtttgatt cacaaaaaca aagcaagccg
     9481 cgataaagca cgtcttgcat caaaacttgc ttaattaaaa aagaaacctt tgggtttcct
     9541 ttttttattt tttccatcat aaggaggcgg gacaaagtgg atcagttttc ccgcgatgac
     9601 taacatcatt aactcgtcaa ccacaaagcc ctcactatga ttatacacct agtgagggct
     9661 ttgtggttgt tttcattatt ctgtgggtga gggcggagcc accatggatg tctttcccgc
     9721 tccctatttt ttattattag ggaagaacac gctcctagag attcaaacta attccttaaa
     9781 accaatctgc cagttcaaat gtataggttt ggtcattata atagtactga tactctccta
     9841 catcgcccaa agcctgaaaa atagcattat tttcagtaga ggccaatgta atcatttgtt
     9901 gcattgcttc tgctgttccg aattgcaaga ctactttatt cgtaccttgt agccgctggt
     9961 cagtgatgga ctgtaaaact gcagcttcat caaagcttgt gaaataaact cccttttgaa
    10021 catagtaatt caatgaatta tctgtacact ttggatagac caatactcta ggttcgtaat
    10081 attgttcttc tccccagtag tcgagtaggt cgctatcagc aattctactt ctctctaata
    10141 tttcatcagg gacacataga taatcgtatg aaatatctgt atgcgcttct ccacttatag
    10201 cttgatcaaa aactggatct ccccaagtcg tatcaacacc atagtattgt ccatttatct
    10261 tgactagatt ccaagcatgc ccaaactcac cgttttgccc attttttgca atccctgtga
    10321 catagataca atcaatgcct gcttttttac atagatattg gaaggtgcga ctatagccag
    10381 cacaaacgga tttcttatct aatagcacac tggtaattcc ttgacttctc cacgtgacag
    10441 atttggtctg cagggcttca aggtcgtaat ccgtttgctt tataatcacc tcgtaaaaat
    10501 atttaacttt ctcatagtcc gacccttttg gtgtctgagc aagtatctta ttcacttcat
    10561 cttctatttt attttgtgtc ggagataaat cactcggata caccggctct tctaaatcca
    10621 atatatcaat tccaccagcc attgctgctt catctgttag ccagtaaaat tctggataat
    10681 catgggcgac agaaaaatat acgcgcgaat atatttcatc attaacagaa tcaatatcga
    10741 tcacttgcat ccgcttgctg agtccattaa caaattggag atagacacga cgttccttat
    10801 cagtatccaa ttgttggtag taaaaatttc cttgaacctc ttcttttaat gctgttaatt
    10861 ctgcttctag ttgctttttt tctttagaaa attcaatttt agctgcttcc aaacgttcac
    10921 tcagctcttg tcctcctcct gaacaagcag aaaggactgt cgccgataat aatatggtta
    10981 aaacgccacg agaaactttc ctcatattta cctccattct tgtttaagta taacataatt
    11041 aggaggcatc tgacaaacta cacaaaacaa aaagggagtg gaaagaattc aatagatcga
    11101 aaaagaattc ccctcacttc caaacattta ggttcgattt tctatggtca ctttcttatt
    11161 tttaggtgac cagctgaaac agttccccaa acctttggag gctggagata gagcgaacag
    11221 agttcgctac aaaactatct gggagacagt tggaagttgg aaatagagcg aactttgaga
    11281 agaaaggctg gattttttac ccagcctcta atcttgatga atggtcagtt atttgggtaa
    11341 acgtacctca aaaatagttc ctttcggttg gttatctctc acagaaatct caccatctaa
    11401 attcttaata atttgttgag ctaaagataa acctaaacca aatccacctt tttgacgtgt
    11461 tctagccttg tcaactcggt aaaagcgatc aaagattttc tttttatcgg catcgctgat
    11521 tcccaaacca ttgtcaataa ctgtaaaaca aactagttta tccttgatat tagctgccat
    11581 ttgaattgtc ccatcatcat cggtatactt catagcgttg tcaaacaaaa tggtgagcaa
    11641 ctgtttaatc aaaactttgt ctgttcgaat tggctgatga atcaaattat taactgttag
    11701 agtttttcca ttttcttcgg caatcataag gtaattagca aagatttcat caaaatagtt
    11761 gggttggaca tcgatcatat cgacttttaa gccatcatca cgacgagcca aattaagtaa
    11821 atttgttgtc aatagtcgca tatttcgaac ttcttctaaa ctagatccga tgctttcact
    11881 actttccaaa atagttgtct cgggatgacg aaatagactt tccaggcgat tttgaagaac
    11941 tgccaacggt gtgcgtaact catgactagc attttcaacg aagtcttttt gtttttgata
    12001 actaattagg attggacgca tgctaagatt cgataggtaa atacttgcta gtatagaaat
    12061 caaccaagct gataccataa caatagcaac tgtcgattca tagctctcaa tggaagattt
    12121 aatttggctg acattcacca aaattgtcgc atattttata tcataggacg aataataccc
    12181 tagttcatct gtagccagcc caatcgttat atagcgataa tcttcagaca taccaaaact
    12241 tgattcaacc gtagtttcct taatttcacc caatttttcc ttgtctaatg ataggtcggc
    12301 caaaccagta aaagtatctg gattaatcat ctctccactt ttactataga gtaaaacatg
    12361 agtattggca cctaacctca gctgatctcc agctctagta tttttttgat ttttaggaac
    12421 aggcgtgcta tctgaactag agctcgtagt ttcttcacca gttggactat cttgaaggat
    12481 aaatacagaa ttgggctcat aggttcttgc gattgcaaaa ccaacagcca gcgctggttc
    12541 tttcttaatg cgtttcaagg tgttatctga attttggtac atagttgaac gcattaattg
    12601 aaaaatgatg gctgtcatta aaccaaaaat cagagtaaat actgcaaaat aacggataaa
    12661 gaaagaaaat ttatccgtgt acattaattt tttaaatcgc ttaggcattt tttagaatgt
    12721 atccgacact acgcagggtt tgaagatttt ccccaaaggt cgttcctttc aactttttcc
    12781 taattttaga aacatatact tctactactg aaattgtagt atcactgtca aagccccaaa
    12841 tacggtcaaa aatttgtgtt tttggcaaaa taacattttg attttgcaag aaataaacta
    12901 gtagatcaaa ttcttttcct agcagttcaa cttcttcgcc atttaccatc gtcatatttg
    12961 tagataggtc aactgtcaca tctccaaagg ttagtgtgtt ttcattaaac ttacccgctc
    13021 gttttaatag agcttgaatt cgcatcttca attcttctaa ataaaacggt tttgtcaaat
    13081 agtcatcagc ccccaattca aaaccatggc ctttatcctc tagactttcc ttggctgttg
    13141 taatcagaac aggtgtcgta acaccttttt cacgtaattc tttcaagact tggaagccat
    13201 ctttttcagg caacatcaaa tccaaaagaa tcaaatcata aacgcctgtt tccgcctcat
    13261 aaacgccctc ttcaccatca aaaacctgca taacatccgc aaaatcatct aagaaatcga
    13321 aaacagaatt tgatagactt aaatcatctt ccactaacaa tattttaatc atcatacact
    13381 tccttacgtt tcggtttaat aaacaaacca aagacctggg acaggtcacg gtctttaaga
    13441 gaaatggtta gataaagtac tactatgacc atctgaaaaa tgaatgataa ggtagtcttt
    13501 atctaactat ttattgatac gagttcatta tatcatggaa taagtaaaaa tcgaaatctc
    13561 aatatttatt gattagaaga actactatct cctgaaattt gagctttaat ttcttgattc
    13621 ttctttttta aagcattcac ttcatcagaa gaagtactcg agtttgactg agttgctcca
    13681 cttgttgcat catgaggggg tgtaccttgt ccaggtggca taccttggct aggacctcct
    13741 ttacctggtg gcatgccttg gggagctcct tgtccattca ttgctgcaga actattttgc
    13801 gccaaacttg cctgactttg tgttgaaact cctgttgagc ttttcgaaat actcattccc
    13861 cctgcaaagc ccaaggtagc agctgaaata gcaacagcag ctagtactgt ttttgactgt
    13921 gacttaaatt gattaataaa tgac
//
LOCUS       DAZQCJ010000049        13866 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000049 DAZQCJ010000000
VERSION     DAZQCJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13866
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.050"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(9..632)
                     /gene="nth"
                     /locus_tag="ACHU66_001283"
     CDS             complement(9..632)
                     /gene="nth"
                     /locus_tag="ACHU66_001283"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR3389569.1"
                     /translation="MVLSKKRARKVIEEIIALYPDAKPSLDFRNHFELVCAVLLSAQT
                     TDAAVNKATPGLFEAFPTPQAMAAAQVKDIEPYISRLGLYRNKAKFLKECAQQLLDRH
                     DGIVPQTREELEALAGVGRKTANVVLSVGFGIPAFAVDTHVGRICKHHDIVKKSATPL
                     ETEKRVMEVLPPELWLPAHQAMIYFGREVCHPKNPECEKFPQLYEFD"
     gene            730..1581
                     /locus_tag="ACHU66_001284"
     CDS             730..1581
                     /locus_tag="ACHU66_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFR3389570.1"
                     /translation="MDLQELCRKVIQARPRNSHKGSYGRVLLIGGLYPYGGAIIMAAL
                     ATVNSGAGLVTVATEKDNIPALHSHLPEAMAFSFDDQALLISSLENADLVLIGPGFGE
                     NSRAEQLLDFVFEHLSDQQILVLDGSALTLVAKQNRKNFPCKQVILTPHQKEWERLSS
                     IPIADQTSQRNLEALQSFQENTILVAKSSATQILKTQPNQVTPIGAGGPYQATGGMGD
                     TLAGMIAGFAVQFPHVQLYERILVATYLHSYIADQLAQDYYLVKPTDVSRNIQKTMLQ
                     FQTQTTA"
     gene            complement(1763..6520)
                     /locus_tag="ACHU66_001285"
     CDS             complement(1763..6520)
                     /locus_tag="ACHU66_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HFR3389571.1"
                     /translation="MKKKETFSLRKYKIGTVSVLLGAVFLFAGAPSVAADELTSLVET
                     KVEATVPDVIVSESASESPVVEELVDTSVEATPTDVTTTDNVEETLGSEALENITNTE
                     VEATQPAVETPAISEKKVEEDEKLAVADETTAITNQEEAKPQNIDSNTIITVPKVWDS
                     GYKGEGTVVAIIDSGLDVDHDVLHISDLSTAKYKSEKEIEAAKEAAGITYGEWFNDKV
                     VFGYNYVDVNTVLKEEDKRSHGMHVTSIATGNPTQPVAEQLMYGVAPEAQVMFMRVFS
                     DLKATTGAALYVKAIEDAVKLGADSINLSLGGANGSVVNMNENVTAAIEAARRAGVSV
                     VIAAGNDGTFGSGHSNPSADYPDYGLVGTSSTARDAISVASYNNTTVGSKVINIIGLE
                     NNADLNYGKSSFDNPEKSSVPFEIGKEYEYVYAGIGQASDFDGLNLTGKLALIKRGTI
                     SFSEKIANATAAGAVGVVIFNSRPGEANVSMQLDDTAIAIPSIFIPLEFGEALAAKPY
                     KIAFNNETDIRPNPKAGLLSDFSSWGLSADGELKPDLAAPGGAIYAAINDNDYANMQG
                     TSMASPHVAGAAVLVKQYLQATYPTKSPQEIEALVKHLLMSTAKAHVNKETTAYTSSR
                     QQGAGIIDTAAAISTGLYLTGEDGYGSITLGNVEDTFSFTVKLHNITNEDKTLNYSTQ
                     LTTDTVQNGLITLAPRLLAEIPGGKVTVKANSSTTVTINVDASSFAEELTGLMKNGYY
                     LEGFVRFTDVADGGDIVSIPYVGFRGEFQNLAVLEEPIYNLIADGKGGFYFEPVTAQP
                     DTVDISHHYTGLVTGSTELIYSTDKRSDFAIKTLGTFKNEAGYFVLELDESGKPHLAI
                     SPNGDDNQDSLAFKGVFLRNYTDLVASVYAADDTERTNPLWESQPQSGNKNFYSGDPK
                     NPKSSIIYPTEWNGTDSEGNALADGKYQYVLTYSSEVPGAAVQTMIFDVIIDRESPVI
                     TTATYDETNFTFNPRPAIEKGESGLYREQVFYLVADASGMTTIPSLLENGDVTVSDNK
                     VFVAQNDDGSFTLPLDLADISKFYYTVEDYAGNISYEKVENLISIGNEKGLVTVNILD
                     KDTNSPVPILFSYSVTDETGKIVAELPRYAGDTSVLKLPFGTYTFDLFLYDTEWSSLA
                     GETKAVVTISEENSTAEVNFYVTLKDKANLLVDIDALLPSGSTIQLVTADGQTIQLPN
                     AKYSKTDYGKFVPVGTYTILPTLPEGYEFLEELDVAVLANQSNVKKLTLINKVALKEL
                     IAELAGLEETARYYNASPELQTAYDKALEDANAVYANKHNQAQVDSAVASLVAAREQL
                     NGQATDTEKLIAEVSNYTPTQANFIYYNAENTKQIAYDTAVRSAQLVLNQENVTQAVV
                     NQALADLLAAKANLDGQKTDISALRSAVSVSSVLKATDAKYLNASENVKQAYDQAVEA
                     AKAILADESASQASVDQALAVLTSAQAELDGVATSTNDAKEPANTATDKKDEGTVTPP
                     PIDSEKVDVQAPPVKDTGNSGHVPIGQKPNPQPTLPRPVTLQASLSSPNQEKQVTQLP
                     NTGDNDTRYYLVLGVIIGLGTLLVSKRRHKEEV"
     gene            6973..7305
                     /locus_tag="ACHU66_001286"
                     /pseudo
     CDS             6973..7305
                     /locus_tag="ACHU66_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935801.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7353..8891)
                     /locus_tag="ACHU66_001287"
     CDS             complement(7353..8891)
                     /locus_tag="ACHU66_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFR3389572.1"
                     /translation="MENHRKEVSFISQSILYSVLRGSLVGIVAGLVVVAFRLAIEKLF
                     SVFTHLYSLATDNAIYLLLIGGLYLVIALLVGKLIKDEPNAKGSGIPQVEAELKGIMD
                     LNWWSVLWRKFIGGVLSIASGLMLGREGPSIQLGAVTAKGVSVLLKSSEMERRSLIAS
                     GAAAGLAAAFNAPIAGLLFVVEEVYHQFSRLVWVSALAASITANFISLHVFGLMPVLH
                     MPKDLQLLSLDQYWIYILLGVFLGLAGYVYEIVILNIQDFYTLLSKIIPLPVPYYSVF
                     AFLFIIPIGYFFSNLLGGGHHLILELPYLEQGLLTLAILILIRFVWSMISYGSGLPGG
                     IFLPILTLGSLLGLFVARFFLDIGFISQEQMLLFIVLGMAGFFAAISKAPLTAIILVT
                     EMVGSLNQLMVIGLVALSSYVMMDLLGGAPVYEAMLEKILPEEVEQQEHMTLIEIVVN
                     ETLDQIFVSELRLPDSCLITSQIHHGKSRIVKGNSQLSMGDSLLVAVPISQIHTVRRI
                     FEGD"
     gene            complement(9005..10075)
                     /locus_tag="ACHU66_001288"
     CDS             complement(9005..10075)
                     /locus_tag="ACHU66_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR3389573.1"
                     /translation="MKRLYSFFVGIIAIVLVLWGISYKLEADSTQGTTDKLVIYNWGD
                     YIDPELLAEFTAETGIQVDYQTFDSNESMYTKIKQGGTTYDLTIPSEYMISKMTEENM
                     LIKLDKSKIEGIENIDPRFMGLSFDKNNDYSIPYFWGTLGIVYNETMVENPPQEWEDL
                     WSEEYRDNIMLIDGVREVMGFGLQSLGYSLNSNNPTEIEKAAEHLYNLTPNVKAIVAD
                     EIKGYMIQDAAAIAVSFSGEASEMLDGNENLRYVVPSKGSNLWFDNMVIPKTAKNIDG
                     AYAFMSFMLRPENALRNAEYVGYSTPIPAAKAMLDEETQNDESFYPSEETMKKMEVYD
                     NLGAEMLGMYNDLYLQFKMYRK"
     gene            complement(10075..10845)
                     /locus_tag="ACHU66_001289"
     CDS             complement(10075..10845)
                     /locus_tag="ACHU66_001289"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR3389574.1"
                     /translation="MKKFANIYLTVAFLLLYLPIFYLIFYAFNEGGDMNGFTGFTLEH
                     FSSMLGDSRLMLILAQTFLLAFLSSLIATIIGTFGAIYIYQAKPRFQNALLSVNNILM
                     VAPDVMIGASFLILFTMIGFQLGFVSVLLSHIAFSIPIVVLMVLPRLKEMNADMISAA
                     YDLGATQPQMLKEIMLPYLTPAIIAGYFMAFTYSLDDFAVTFFVTGNGYSNLSVEIYS
                     RARQGISLEINALSTLVFLFSILLVVGYYFISREKEAN"
     gene            complement(10845..11645)
                     /locus_tag="ACHU66_001290"
     CDS             complement(10845..11645)
                     /locus_tag="ACHU66_001290"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR3389575.1"
                     /translation="MKKTSRLFAIPYALWVFLFVLAPVALIIFKSFFDIHGNFTLANY
                     QTYFNSPNMTYLRMSFNSIFYAGIITLVTLLVSYPTAYFLTKLKHRQLWLMLIILPTW
                     VNLLLKAYAFIGIFGQHGSINQFLEFIGLGAQQILFTDFSFIAVAAYIEIPFMILPIF
                     NALDDLDKNLINASRDLGATPWQTFTKVIFPLSMNGVRSGVQAVFIPSLSLFMLTRLI
                     GGNRVITLGTAIEQHFLTTQNWGMGSTIGVVLILAMLLIMWMTKERKK"
     gene            complement(11629..12783)
                     /locus_tag="ACHU66_001291"
     CDS             complement(11629..12783)
                     /locus_tag="ACHU66_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3389576.1"
                     /translation="MKKPIIEFKNVSKVFEDSGTTVLKDISFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDASSGDIYLDGQRINDVPTNKRDVHTVFQSYALFPHMTVFENVAF
                     PLKLRKIDKAEIERRVTEALQMVRLSGYENRSIQKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGRMIEDYLVEFNGKRFEAVDGGMRPNEEVEV
                     VIRPEDLQITLPEEGKLQVKVDTQLFRGVHYEIIAYDDLGNEWMIHSTRKAIVGEVIG
                     LSFEPEDIHIMRLNETEEEFDARIEEYVEVEEVEDGLINAIEEERNEENL"
     gene            complement(12822..13730)
                     /gene="murB"
                     /locus_tag="ACHU66_001292"
     CDS             complement(12822..13730)
                     /gene="murB"
                     /locus_tag="ACHU66_001292"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR3389577.1"
                     /translation="MKDKIRLELEGIDIRFDEPLKEYTYTKVGGAVDYLAFPRNRYEI
                     VRIVEFAKREGIPWQVLGNSSNIIVRDGGIRGFVIRMDKLNSVTVSGYTIEAEAGANL
                     IETTKVALFHSLSGFEFACGIPGSIGGAVYMNAGAYGGEVAHILVSAQILTPAGYVET
                     LDNRELRFGYRSSILQENGAIVLSAKFALKPGNHTVIQQEMARLTHLRELKQPLEYPS
                     CGSVFKRPLGHFAGQLIMDAGLKGYRIGGVEVSEKHAGFMVNIENGTASDYENLIAHV
                     IEVVEKSSGITLEREVRIIGDPADTL"
BASE COUNT         4169 a         2966 c         2685 g         4046 t
ORIGIN      
        1 aagatttttt agtcaaattc atataattgt gggaattttt cgcattcagg gttcttagga
       61 tggcaaacct cacgaccaaa ataaatcata gcttgatgag cagggagcca gagttcagga
      121 ggtaagactt ccatgactcg tttttctgtt tccaggggtg tagccgattt tttgacaata
      181 tcgtggtgtt tacaaatccg tcccacgtgg gtatcaactg cgaaggcggg aattccgaaa
      241 ccgacactta aaacaacatt ggccgttttt cttcccactc ctgccaaggc ttctaattcc
      301 tcacgagtct gtgggactat gccatcatgt cggtccaaca actgttgggc acattctttt
      361 aaaaactttg ctttattacg atataagccc aagcgagata tatagggctc gatgtccttc
      421 acttgtgctg ctgccatggc ttgaggcgtt gggaaagcct cgaacaaacc tggggttgcc
      481 ttattaacgg cagcatccgt tgtctgagct gacaaaagta ctgcgcagac caattcaaaa
      541 tggttgcgga aatccagact aggcttagca tcaggataga gggcgatgat ttcttcaata
      601 acttttcttg cacgtttttt tgataatacc atgaattcat tatagcatga aaacttggat
      661 ttatctaaaa tctggattgt cataaacctc attcctcgtg gtataataag aagaaaaggg
      721 ggaatatgta tggacttaca agaactctgt agaaaggtca ttcaagcaag gccacgcaac
      781 agccataagg gaagctatgg tcgagtcttg ctgattggtg gactgtatcc ctatggtgga
      841 gcgattatca tggccgcact agcaaccgtc aacagtggag caggcttggt gactgttgct
      901 actgaaaaag ataacatacc agccttacat agtcatctgc ctgaagccat ggcattttca
      961 tttgacgatc aagccttgct gataagcagc ttagaaaatg ctgacttggt attgattggt
     1021 ccaggttttg gggaaaatag cagagctgaa cagttattgg actttgtttt tgaacattta
     1081 tctgaccagc agattttagt tcttgacggc tcagccctta ccttagtcgc aaagcaaaac
     1141 agaaagaact tcccgtgtaa acaagtcatc ctgacacccc accagaaaga atgggaacgg
     1201 ttatcatcca ttccgattgc tgaccaaaca agccaaagaa atcttgaagc cttgcaatct
     1261 tttcaagaaa atacaattct tgtagctaaa agttctgcta cacaaatcct aaaaacccag
     1321 ccaaaccaag tcaccccaat tggagctggt ggtccttacc aagcaacagg aggtatggga
     1381 gatactcttg caggtatgat tgcaggattt gctgtacaat ttccccatgt tcaactatat
     1441 gaaagaatcc ttgtagcaac ctacctccac tcctatattg ccgaccaact ggctcaagat
     1501 tattatcttg taaagccaac tgatgtctca agaaacatcc aaaagacaat gcttcaattt
     1561 caaacacaaa caaccgccta atcaatgatt aagcggtctt ttatataaca aactcgtgat
     1621 taaagcctga caaatggtag aaaatgatac tcaatgcaaa tcaaaagtag cctaggaaac
     1681 gaagccgaag atagaactgg agttcatcaa ggcaagttga caacggataa tttagatttt
     1741 cgaagagtat gactatattt tcttagactt cttctttatg acgtcgtttg cttaccaaca
     1801 gagtccctag cccaataatg acaccaagaa caagatagta tctcgtatca ttgtctccag
     1861 tatttggtag ttgagtcacc tgtttttctt gattagggct agatagacta gcttgcaaag
     1921 tgaccggacg aggtaaagtt ggttgagggt ttggcttctg acctatcggt acatgccctg
     1981 aattcccagt atcttttaca ggaggtgcct gtacatcaac tttttctgag tctataggtg
     2041 gaggcgttac agtgccttca tcttttttgt cagtggcagt atttgctggc tctttggcat
     2101 catttgttga agtagcaaca ccatccagtt ctgcctgagc gcttgtcaga acggctagag
     2161 cttgatcgac acttgcttgg cttgcagatt catcagctag aatcgctttc gctgcttcaa
     2221 ctgcctggtc ataagcttgt ttcacgtttt cagatgcatt gagatactta gcatctgtcg
     2281 cttttaatac ggaagaaacg gatactgcgc tacgaagggc tgaaatatca gtcttttgac
     2341 catctaagtt ggctttcgcc gctaacaagt ccgccaacgc ttggttgaca actgcctgtg
     2401 ttacattctc ttggttcaat acaagttgtg ctgaacgaac agctgtatca taggcaattt
     2461 gtttggtatt ttcagcattg taataaataa agtttgcctg agtcggtgtg tagtttgata
     2521 cttcagcaat tagtttttcc gtatcggtag cctgaccgtt tagctgttct ctcgccgcca
     2581 caagactggc aactgctgaa tctacttgtg cctgattgtg tttgttggca tatactgcat
     2641 tggcatcttc taatgcttta tcataggcag tttgaagttc tggactagca ttgtaataac
     2701 gcgctgtttc ttcaagtccc gcaagttcag caatcagttc tttcaaagca actttattaa
     2761 tcaaggttaa tttcttaaca tttgactggt ttgcaagtac tgctacgtct aattcttcca
     2821 aaaattcata gccttctggg agggttggaa ggatagtgta ggtaccaact ggtacaaatt
     2881 taccataatc ggtcttagaa tatttagcat ttggtagctg aatagtctgg ccatcagcag
     2941 ttaccagttg gatggttgaa ccagaaggta gtaatgcatc aatatctacc agcaagttgg
     3001 ccttatcttt caaagtcaca tagaaattca cctcggcagt gctattttct tccgaaatcg
     3061 tcacgactgc ttttgtttca cctgctaggc ttgaccattc tgtatcatat aagaataaat
     3121 caaaggtgta agtaccaaat ggtagcttaa gaacgctagt atcgccggca tatcgtggta
     3181 attctgcaac aatcttgcct gtttcatctg tgactgagta agagaaaagt attggtacag
     3241 gactatttgt atctttatca agaatattga cagttaccaa ccctttttca ttgccgatac
     3301 tgatcagatt ctctactttt tcatagctga tgttaccagc ataatcctct actgtgtagt
     3361 agaattttga aatatctgca aggtcaagag gcaatgtaaa ggagccatcg tcgttttgtg
     3421 ccacaaatac cttgttatca gaaacggtta catcaccatt ttctaataag gaaggaatag
     3481 ttgtcatacc gcttgcatct gctacaagat agaatacttg ctcgcgatat agcccggatt
     3541 ctcctttttc aatggctgga cgagggttaa atgtaaagtt tgtttcatca taggtagctg
     3601 tggtgataac tggtgattct ctatcgatga taacgtcgaa aatcatagtt tgtactgctg
     3661 cacctggaac ttcagatgag taggtcaaaa cgtattgata cttaccatct gctaaagcat
     3721 ttccctcgct gtctgtccca ttccattcag tagggtaaat aatgcttgat tttggatttt
     3781 taggatcacc actatagaag ttcttattgc ctgactgtgg ttgactttcc caaagtggat
     3841 ttgttcgttc ggtatcatct gcagcataga cgcttgcgac taaatccgta taatttctca
     3901 agaagacacc tttgaaagcg agcgaatctt ggttgtcatc cccatttggc gagatagcta
     3961 aatgaggctt accagactca tcaagctcta aaacaaaata tcctgcttca tttttaaatg
     4021 taccaagtgt cttgatcgca aagtcagatc gtttgtctgt agaatagatt aactccgtac
     4081 ttcctgtaac aagacctgtg tagtgatggc tgatgtcaac agtatctggt tgtgctgtaa
     4141 caggttcaaa gtagaagccc cccttaccat cggcaataag attgtaaatc ggctcttcta
     4201 gaacggctag attttggaat tccccacgga aaccaacgta tggaatgctg acaatatcac
     4261 cgccatcggc tacatctgta aatcgaacaa aaccttcaag atagtaaccg tttttcatta
     4321 aacctgtcaa ttcttctgca aagcttgatg catcgacatt aattgtaaca gttgtacttg
     4381 aattggcttt cacagtcacc ttaccgccag gaatctctgc taatagacgc ggagccaagg
     4441 tgatcaatcc gttttggaca gtatccgttg ttaattgcgt tgagtagttt aaagtcttat
     4501 cttcgtttgt aatgttatga agtttgaccg taaagctgaa tgtatcctca acatttccca
     4561 aggtaatgct gccataaccg tcttcgccag tcaaatataa acctgtagaa atagctgccg
     4621 cagtatcgat gatacctgca ccttgttgac gagaggaagt gtaggctgtt gtttctttgt
     4681 tcacatgcgc tttagcagta gacataagca agtgttttac taaggcttcg atttcttgag
     4741 gggacttagt agggtaagtt gcctgtaaat attgttttac tagtacggct gctcctgcta
     4801 cgtgtggtga agccatactt gttccctgca tgttggcata gtcattatca ttgatggctg
     4861 cataaatagc accacctgga gcagctaagt ctggttttag ctcgccatcc gctgatagac
     4921 cccaacttga aaaatctgaa agaagacctg ctttggggtt aggacgaatg tctgtttcgt
     4981 tattgaacgc aatcttatat ggtttagccg ctaaagcttc accgaattcc aatggaatga
     5041 agatagatgg aattgcgata gctgtatcat ccagttgcat gctcacattg gcttcacctg
     5101 ggcggctatt gaaaataacg acccctactg cccctgcagc tgttgcattg gctatttttt
     5161 ctgagaaact gatggttcct cgtttaataa gcgcaagttt tccagtcaaa tttaaaccat
     5221 caaaatccga agcttgaccg attcccgcat agacatattc atattctttc ccgatttcaa
     5281 atggtacgga acttttctct ggattatcaa acgaactttt accgtaattc aagtcagcat
     5341 tgttttctaa gccaatgata ttaattactt tactaccaac cgttgtgtta ttgtaagaag
     5401 cgacagaaat tgcatcgcga gctgttgaag atgtaccgac caagccataa tctggataat
     5461 cggctgacgg attagaatga ccggaaccaa atgttccatc attaccagct gcaataacaa
     5521 cagaaacccc tgcacgacga gcagcctcga ttgctgcagt cacattttca ttcatgttaa
     5581 caacagagcc attagctcct cccaggctga ggttgatgct atctgcacct aattttacag
     5641 catcttcaat cgcctttacg tacaatgctg cgcctgttgt agctttgagg tctgagaata
     5701 cacgcataaa catgacttgc gcttcaggag ctacaccata cattaattgt tcagcgactg
     5761 gttgtgtcgg atttcctgtg gcaatactcg ttacgtgcat accgtgtgag cgtttgtctt
     5821 cttctttcaa gacagtattc acatcaacat agttataacc aaatacaacc ttatcgttaa
     5881 accattcacc gtaggtaatt cctgctgctt ctttagctgc ttctatctct ttttctgatt
     5941 tatattttgc agttgagaga tctgaaatat gcaatacatc gtggtcaacg tcaagacctg
     6001 aatctatgat tgcaactact gttccttcgc ccttgtaacc actatcccaa actttaggta
     6061 ctgtaatgat cgtattgcta tcaatgtttt gtggttttgc ttcttcctga ttagtaatag
     6121 cagtagtctc atctgctacg gcaagcttct catcttcttc tacttttttc tctgaaatag
     6181 ctggagtttc tacagctggt tgagtcgctt ctacttctgt atttgtgatg ttttcaagag
     6241 cttctgagcc aagtgtttcc tctacattat ctgtagtggt tacatcagtt ggggtagcct
     6301 ccacagaagt gtcaactaac tcctcgacta cgggactttc tgaggctgat tcgctgacga
     6361 ttacgtcagg aacagttgct tccaccttag tctctacaag gcttgtcaat tcatctgcag
     6421 ctaccgatgg tgcacctgca aacaaaaaaa ctgcacccaa aagaacagac acagttccaa
     6481 ttttatactt ccgaagtgag aaagtttctt tctttttcat aacttttcct cctagttttt
     6541 ctccttcatt attggcaaaa ccaataagta tattaaaaca taaaactatc agcattgcaa
     6601 gtatattctg aaaattcagt tatttattca aactataact aggtatcaat aatatctata
     6661 accatagata cagataatac acactaaaaa gggagtgagc aagacatcca tgatggctcc
     6721 gtccttaccc acagaataat gaaaataagc ataaagccct cactaagatt atatacctag
     6781 tgagggcctt gcggttggta cattattgat gtgaattatg atgacaaaac tgctccagcc
     6841 tactgataag atccacaata tacactcaag aatatgacta aaattcagta gataggctct
     6901 ctaacttata aggaaggaat tgtcatagat aatcatgtgt agatcaagtc gatattgttg
     6961 tttgtattcc cctcaacttg tccaaagcgt tacttagcta catttgatac aacgactaaa
     7021 ggtctacgtg cttaccacga ttttctcagt caatttcatg tggaagctgt tggtatggaa
     7081 agcaccggtg tctattggcg acctgtctgg catgctctat gtgaggactt cgagctaata
     7141 cttgctcaac cagcccacat gaaggccatt acaggtcaga aaactgacaa gaaagacgct
     7201 tactggactg ccaaatgaat gcggattggt ctgcttcctc ggagtttcgt tcccgatgaa
     7261 atcattcctg aattgagaga gttgaccaga caacagaaac attataatat tgggactttt
     7321 ctcatctctt tgttaagaca atctaaaaaa tgctaatctc cctcaaatat cctacgaacg
     7381 gtatgaattt gagagattgg aactgcaacg agcaaggaat cccccataga aagttggcta
     7441 tttcctttca caatccttga cttaccgtga tgaatttgac tggtgatcaa gcatgaatcc
     7501 ggcaaacgta actcgctaac aaatatttga tccaatgttt cgttgacaac aatctctatt
     7561 aaggtcatgt gttcttgctg ttctacttct tctggcagaa ttttttccaa cattgcttca
     7621 tagactggcg cgccacctaa gaggtccatc atcacatagg aactcaaagc taccaacccg
     7681 atgaccatca attgattcaa acttccaacc atttcagtta ctaaaataat agcagttaga
     7741 ggtgctttgg aaatcgctgc gaaaaagcct gccattccca atacaataaa caatagcatc
     7801 tgctcctgac tgatgaaacc aatatcaagg aagaaccgtg cgacaaaaag cccaaggaga
     7861 cttcctaagg ttaatatggg caaaaagatg ccccctggta gaccagaacc ataggaaatc
     7921 atgctccata caaatcgtat taaaatcaag attgccaatg tcagtagtcc ttgttccaaa
     7981 tatggaagct ctaaaatcaa atgatgcccg ccacccaaca gattagagaa aaaataacca
     8041 atcggtataa taaacaaaaa ggcaaataca ctgtagtatg gaactgggag aggaatgatt
     8101 tttgagagga gggtatagaa atcttgtata ttgaggataa caatttcata cacatatcca
     8161 gcaagtccaa ggaaaactcc aagtaagata tagatccaat attggtcaag agatagtaac
     8221 tgtaagtcct tcggcatatg caaaacaggc attaatccaa aaacatgtaa ggaaataaag
     8281 ttcgcagtga tactggctgc cagggctgag acccatacca gacgtgaaaa ctgatgatag
     8341 acttcctcca caacaaataa aaggcctgct attggtgcat tgaaagctgc tgccaaacct
     8401 gccgcagcgc cactagcaat caaactcctt ctttccattt cactggattt aagaagaact
     8461 gatacacctt tagccgttac agctcctaat tgaatactgg gaccttcacg ccccagcatt
     8521 agaccagatg caatggataa aacaccgccg ataaattttc tccaaaggac tgaccaccag
     8581 tttaaatcca taattccttt tagttctgcc tccacctgag gtataccaga accttttgca
     8641 tttggctcgt ctttgatcaa cttgccaaca agtaaagcta tgaccagata aagccctcca
     8701 ataagaagta aatagattgc attgtctgtt gccagagaat agaggtgcgt aaagacagaa
     8761 aatagttttt caatggctaa gcgaaatgca acgaccacta aaccagcgac aatgccaact
     8821 aaactaccac gcagaaccga atagaggatt gattgagaaa taaaactaac ttctttccgg
     8881 tgattttcca ttgggatttc ccttcctttc tatagtatgt atgaccaaaa aataggagca
     8941 ataagtactt agtaaagaac ctcactccca ttcttttatt tatattgcta cctgtccaat
     9001 cagactattt ccgatacatc ttgaattgga gatagaggtc attatacatt ccaagcatct
     9061 ctgctccaag attatcataa acttccatct tcttcatcgt ttcttctgaa ggatagaagg
     9121 attcgtcgtt ctgagtctcc tcatccagca tagccttggc tgcaggaatt ggtgttgagt
     9181 agccaacata ttctgcattg cgaagcgcat tttcaggacg aagcataaaa ctcataaagg
     9241 cgtaagctcc atcaatattt tttgctgttt ttggaatgac catgttatca aaccacaggt
     9301 tagaaccttt tgatggtaca acatagcgca gattttcatt tccatctaac atttcactcg
     9361 cctcacctga gaacgaaaca gcaattgctg ccgcatcttg tatcatataa ccttttatct
     9421 catcagcaac aattgctttg acatttggag ttaaattata aagatgttcg gctgcctttt
     9481 caatctcggt aggattgtta gaattgagac tatagcccag tgattgtaga ccaaagccca
     9541 tgacttcacg cacaccatca attaacatga tattgtcacg gtattcctct gaccacaagt
     9601 cttcccattc ctgaggagga ttttctacca tggtttcatt gtaaacaatg cccaaggttc
     9661 cccagaaata tggaatagaa tagtcattat tcttatcaaa gctaaggccc ataaatcgtg
     9721 gatcgatatt ttctatccct tcaatcttag acttatctag cttgattaac atattttctt
     9781 ctgtcatctt agaaatcatg tactcagatg ggattgtcaa atcgtatgtt gttccaccct
     9841 gcttaatctt ggtgtacatc gattcattgg agtcaaaggt ctgataatct acctgaatac
     9901 ctgtttcagc agtgaattct gcgagtaatt ctgggtcaat ataatcaccc cagttataga
     9961 tgacaagttt atcagttgtc ccttgggttg aatcagcttc taacttataa ctaattcccc
    10021 ataaaactag gacaatagct ataattccta caaaaaatga atacaatcgt ttcattagtt
    10081 ggcctccttt tcacgtgaga tgaaataata acccactacc aataagatag agaaaaggaa
    10141 aacaagggtt gatagggcat tgatttccaa agaaatccct tgacgagccc gtgagtaaat
    10201 ctctacagat aggtttgagt aaccatttcc cgtaacaaag aatgtcacgg caaagtcatc
    10261 caaggaatag gtaaaggcca tgaagtagcc tgcaatgatg gctggtgtta ggtatggcaa
    10321 catgatttct ttcagcattt gtggctgagt tgcccccaaa tcataagctg ctgaaatcat
    10381 atcggcattc atttccttca agcgtggcaa aaccatcaat actacgattg gaatagaaaa
    10441 agcaatatga ctgagcaata ctgagacaaa gccaagctgg aaaccaatca tggtaaagag
    10501 aattaggaaa ctcgccccaa tcatgacgtc tggagctacc atcaagatat tattgacaga
    10561 caagagtgca ttttgaaaac gaggttttgc ctgatatata tagatggcac cgaaagttcc
    10621 gataatggtc gcaatcaaag aactcaagaa ggccaacaag aaggtttgag ccagaatcag
    10681 cattagtcgg ctatcgccaa gcatagatga aaaatgctct aaggtaaagc ctgtaaagcc
    10741 attcatatct ccgccttcat taaaggcata aaaaataaga tagaaaatcg gtaagtagag
    10801 aaggagaaag gcaactgtta agtagatatt tgcaaatttc ttcattattt cttcctctct
    10861 ttcgtcatcc acataatcaa gagcatggcc aaaatcaaga ccacacctat ggttgaaccc
    10921 attccccagt tttgtgttgt gagaaagtgt tgttctatag ctgtaccaag cgtgatcact
    10981 cggttaccgc caatcaaacg ggtcaacatg aagaggctca gacttggaat gaaaaccgcc
    11041 tgtacaccag aacggacacc attcatagaa agaggaaaaa taacttttgt gaaggtctgc
    11101 caaggagttg cccccaaatc acgactggca ttgattaggt ttttatccaa atcatccaag
    11161 gcgttaaaga tcggcaaaat cataaacgga atctcgatat aggctgcaac agcaataaat
    11221 gagaaatccg tgaaaagaat ttgctgagct cctagaccaa taaattctag gaactggttg
    11281 atagagccgt gttgtccaaa aataccgata aaggcatagg ctttcagtaa aagattgacc
    11341 caggttggca agataatcaa catgagccag agctgtctat gcttgagttt ggtcaagaaa
    11401 taggctgttg ggtaggaaac caaaagcgtg accagagtaa taatacctgc gtaaaaaatg
    11461 gagttaaaac tcattcgcag ataggtcata ttgggcgagt taaaataggt ctggtaattg
    11521 gccaaagtga aattgccatg aatatcaaag aaagatttga agataatcaa ggcaactgga
    11581 gcgagtacaa agagaaagac ccatagggca tagggaattg caaaaagcct agaggttttc
    11641 ttcattgcgt tcctcctcaa tggcattaat caagccatct tctacttctt ccacttccac
    11701 atattcttcg atacgggcat cgaattcttc ctctgtttca ttgagacgca tgatatgaat
    11761 atcttctggc tcaaaactta gtccaatcac ttctccgaca atagccttac gagttgagtg
    11821 aatcatccac tcattaccga ggtcgtcata ggcgatgatt tcatagtgaa ccccacggaa
    11881 gagttgagta tccactttta cctgcaattt gccttcctca ggtagggtaa tctgcaaatc
    11941 ttctggacgg ataacaactt caacttcttc attaggacgc ataccgccat cgacagcttc
    12001 aaagcgtttg ccgttaaact caaccagata gtcttctatc atacgacctg gaaggatatt
    12061 ggattcaccg atgaaggtag caacaaaatg gttgattggc tcgtcgtaaa tgtccactgg
    12121 cgtgccagac tgaacaattt caccttcatt catgacaaaa atccagtcac tcatggccag
    12181 agcttcttct tggtcatggg taacgaagac gaaggtaatt cctagacgtt gttgcaattc
    12241 acgcagttcg tactgcattt ctgttcttag tttcaagtcc agggcagaaa gtggttcatc
    12301 tagcaagaca acgcgaggct ggttgataat cgcacgtgca atagcaaccc gctggcgttg
    12361 accaccagac aatttctgaa tggaacgatt ttcataacca gaaagtcgga ccatttgtaa
    12421 ggcttctgtc acacggcgtt caatttcagc cttgtcaatc tttcgtaatt taagaggaaa
    12481 agccacattt tcaaagacag tcatatgtgg gaaaagggca taggactgga aaacagtatg
    12541 gacatcccgt ttattggtcg gaacatcatt aatccgttga ccgtccagat aaatatcacc
    12601 tgaactcgca tccaataaac cagcaataat gttcaaaatt gttgatttcc ctgaaccaga
    12661 agctcctaaa agggtataaa atttcccctc ttccaattca aaactaatat cctttaatac
    12721 agttgttcca ctgtcttcaa atacttttga aacattctta aattcaataa ttggcttttt
    12781 caattctcat aaattccttc ttttttgtaa gagcctgatg gctagagtgt atcagcagga
    12841 tcgccgataa tacgaacctc tctctccaga gtaattcctg aagatttttc aacaacttca
    12901 attacatggg caatcaaatt ttcgtaatca gatgctgtac cattttctat attgaccata
    12961 aagccagcgt gtttttcaga aacttcgact ccaccaattc gataaccttt taaacccgca
    13021 tccataatca actgtcctgc gaagtgacca agtggacgct tgaagactga accacaagat
    13081 ggatattcca aaggttgctt gagctcgcgt aaatgtgtca aacgagccat ttcttgttga
    13141 ataaccgtat gatttcctgg cttgagggca aattttgcag acaaaacaat cgcaccgttt
    13201 tcctgcaaaa tagaagaacg gtagccaaat cgtaactcac gattgtccaa tgtttcgaca
    13261 taacctgctg gagtcaaaat ctgcgctgaa actagaatgt gtgccacttc acctccgtaa
    13321 gcacctgcat tcatgtagac agcaccacca atgcttcccg gaataccaca agcaaattca
    13381 aatcctgata aagaatggaa aagtgcgact tttgttgttt caatcagatt ggcacctgct
    13441 tctgcttcaa tggtgtatcc tgatacagta acggagttaa gtttatccat acggataaca
    13501 aaaccacgaa taccgccatc tcggacaatg atattacttg aatttcctaa tacttgccat
    13561 ggaatacctt cgcgtttagc aaattctact atacgaacaa tctcgtaacg attccgcgga
    13621 aaagctagat aatcaacagc tcctcctact tttgtatagg tatattcttt taaaggctca
    13681 tcgaatcgaa tatcgatgcc ttctaattca agcctaattt tatctttcat aaccttctca
    13741 tttcaattta agatatacag gatttattat accaaaaatt acaggagaaa acgaataaaa
    13801 ttcaacaaaa atactttcac aaaccaaatt aatttgcccc aatattttga attggagaat
    13861 ggaatg
//
LOCUS       DAZQCJ010000050        13575 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000050 DAZQCJ010000000
VERSION     DAZQCJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13575
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.051"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(1..229)
                     /locus_tag="ACHU66_001293"
                     /pseudo
     CDS             complement(1..229)
                     /locus_tag="ACHU66_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(433..1775)
                     /locus_tag="ACHU66_001294"
                     /pseudo
     CDS             complement(433..1775)
                     /locus_tag="ACHU66_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(1729..1935)
                     /locus_tag="ACHU66_001295"
     CDS             complement(1729..1935)
                     /locus_tag="ACHU66_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR3389578.1"
                     /translation="MDELLAIIKQQTSTIEQLTNELTLLREQVDYLRQKLYGKSSEKV
                     VYQPGQLSLFGEESLLEEEADLPS"
     gene            complement(2052..2339)
                     /gene="tnpB"
                     /locus_tag="ACHU66_001296"
     CDS             complement(2052..2339)
                     /gene="tnpB"
                     /locus_tag="ACHU66_001296"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR3389579.1"
                     /translation="MTIRLSDLGQVFLVCGKTDMRQGIDSLAYLVKSQFNLDPFSGQV
                     YLFCGGRKDRFKALYWDGQGFWLLYKRFENGKLTWPNNEEEVKSLTSEQVD"
     gene            complement(2320..2505)
                     /locus_tag="ACHU66_001297"
     CDS             complement(2320..2505)
                     /locus_tag="ACHU66_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389580.1"
                     /translation="MSQPVVQLTIPQSRRFEKKSRNDILMKIRLGKTEVTFFQSINPE
                     VMETILDKVLHYDHPTQ"
     gene            complement(2741..3614)
                     /locus_tag="ACHU66_001298"
                     /pseudo
     CDS             complement(2741..3614)
                     /locus_tag="ACHU66_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681261.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3811..5043)
                     /locus_tag="ACHU66_001299"
     CDS             complement(3811..5043)
                     /locus_tag="ACHU66_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HFR3389581.1"
                     /translation="MEPICLIPARSGSKGLPNKNMLFLDGVPMIFHTIRAAIESGCFK
                     KENIYVSTDSEVYKEICETTGVQVLMRPADLATDFTTSFQLNEHFLQDFSDDQVFVLL
                     QVTSPLRSGKHVKEAMELYGKGQADHVVSFTKVDKSPTLFSTLDENGFAKDIAGLGGS
                     YRRQDEKTLYYPNGAIYISSKQAYLADKTYFSEKTAAYVMTKEDSIDVDDHFDFTGVI
                     GRIYFDYQRREQQNKPFYKRELKRLCEQRVHDSLVIGDSRLLALSLDGFDNISIGGMT
                     ASTALENQGLFLATPIKKVLLSLGVNDLITDHPLHMIEDTIRQLMESLVSKAEQVFVT
                     TIAYTLFRDSVSNEEIVQLNDVIVQSASELGISVIDLNEVVEKEAMLDYQYTNDGLHF
                     NQIGQERVNQLILTSLTR"
     gene            complement(5052..5702)
                     /locus_tag="ACHU66_001300"
     CDS             complement(5052..5702)
                     /locus_tag="ACHU66_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR3389582.1"
                     /translation="MIDRGESLMKKVAFLGAGTFSDGVLPWLDRSRYELIGYFEDKPI
                     SDYRGYPVFGPLQDVLTYLDDGKVDAVFVTIGDNVKRKEIFDLLAKDHYDALFNIISE
                     QANIFSPDSIKGRGVFIGFSSFVGADSYVYDNCIINTGAIVEHHTTVEAHCNITPGVT
                     INGLCRIGEGTYIGSGSTVIQCIEIAPYTTLGAGTVVLKSLTESGTYVGVPARKIK"
     gene            complement(5692..6825)
                     /gene="neuC"
                     /locus_tag="ACHU66_001301"
     CDS             complement(5692..6825)
                     /gene="neuC"
                     /locus_tag="ACHU66_001301"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFR3389583.1"
                     /translation="MKKICFVTGSRAEYGIMRRLLSYLQDDPEMELDLVVTAMHLEEK
                     YGMTVKDIEADKRRIVKRIPLHLTDTSKQTIVKSLATLTEQLTVLFEEVQYDLVLILG
                     DRYEMLPVANAALLYNIPICHIHGGEKTMGNFDESIRHAITKMSHLHLTSTDEFRNRV
                     IQLGENPNHVLNIGAMGVENVLKQDFLTREELAMELGIDFAEDYYVVLFHPVTLEDNT
                     AEEQTQALLDALKEDGSQCLIIGSNSDTHADKIMELMHEFVKQDSDSYIFTSLPTRYY
                     HSLVKHSQGLIGNSSSGLIEVPSLQVPTLNIGNRQFGRLSGPSVVHVGTSKEAIVGGL
                     GQLRDVIDFTNPFEQPDSALQGYRAIKEFLSVQASTMKEFYDR"
     gene            complement(6837..7853)
                     /gene="neuB"
                     /locus_tag="ACHU66_001302"
     CDS             complement(6837..7853)
                     /gene="neuB"
                     /locus_tag="ACHU66_001302"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFR3389584.1"
                     /translation="MVYIIAEIGCNHNGDVHLARKMVEVAVDCGVDAVKFQTFKADLL
                     ISKYAPKAEYQKITTGESDSQLEMTRRLELSFEEYLDLRDYCLEKGVDVFSTPFDEES
                     LDFLISTDMPVYKIPSGEITNLPYLEKIGRQAKKVILSTGMAVMDEIHQAVKILQENG
                     TTDISILHCTTEYPTPYPALNLNVLHTLKKEFPNLTIGYSDHSVGSEVPIAAAAMGAE
                     LIEKHFTLDNEMEGPDHKASATPDILAALVKGVRIVEQSLGKFEKEPEEVEVRNKIVA
                     RKSIVAKKAIAKGEVFTEENITVKRPGNGISPMEWYKVLGQVSEQDFEEDQNICHSAF
                     ENQM"
     gene            complement(8375..9781)
                     /locus_tag="ACHU66_001303"
     CDS             complement(8375..9781)
                     /locus_tag="ACHU66_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFR3389585.1"
                     /translation="MSKKSIVVSGLVYTIGTILVQGLAFITLPIYTRVISQEVYGQFS
                     LYNSWVGLVGLFIGLQLGGAFGPGWVHFREKFDDFVSTLMVSSIAFFLPIFGLSFLLS
                     QPLSLLFGLPDWVVPLIFLQSFMSVVQGFFTTYLVQRQQSMWTLLLSVLSAVINTALS
                     LFLIFSMENDFIARVMANSATTGVFACVSLLFFYKKIGLHFRKDYLRYGLSISIPLIF
                     HGLGHNVLNQFDRIMLGKMLTLSDVALYSFGYTLASILQIVFSSLNTVWCPWYFEKKR
                     GADKDLLSYVSYYLAIGLFVTFGFLTIYPELAMLLGGSEYRFSMGFIPMIIVGVFFVF
                     LYSFPANIQFYSGNTKFLPIGTFIAGVLNISVNFVLIPTIGIYGAALATTASYLLLLV
                     LHYFVAKKKYAYDEVAISTFVKVIALVVVYTGLMTVFVGSIWIRWSLGIAVLVVYAYI
                     FRKELTVALNTFREKRSK"
     gene            complement(9790..10761)
                     /locus_tag="ACHU66_001304"
     CDS             complement(9790..10761)
                     /locus_tag="ACHU66_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 52"
                     /protein_id="HFR3389586.1"
                     /translation="MRRIYICHTMYQILISLLKMDVERDSLMSVDIIGHFPDVREQLQ
                     QHVHLIEETERSFDLYSLIARSKTKERLSLLQSYDEVIIFQDHRQVGHFLNKHRIPYS
                     LLEDGYNFFKDKRVCDLESIQSSVWKRLFYQWYFKPTYLIGSSLYCQSIEVNDLSLVQ
                     FDEAYKPFVEVPRKQLFDQASPEKVQALLQIFGARAIVADEESSQKRLLLLTQPLSWD
                     YHVTEEELLEIYVAGLAPYREDYTIYIKPHPRDGVDYSFLGKAVVLLPQGIPFELFEM
                     AGSIRFDIGMTYSSSALDFLNCFEEKVYLKDTFPLLSKNDILREGIE"
     gene            complement(11198..11377)
                     /locus_tag="ACHU66_001305"
                     /pseudo
     CDS             complement(11198..11377)
                     /locus_tag="ACHU66_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
     gene            complement(12253..12564)
                     /locus_tag="ACHU66_001306"
     CDS             complement(12253..12564)
                     /locus_tag="ACHU66_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389587.1"
                     /translation="MNPTNSRIALFDTIKCIMVLCVIFTHLDWSVEQRQWFIFPYFVD
                     MAVPIFLLLSAYFRTNKWNTKQETLKLKFSSGIKESINMLCLYAIVMAVNVLLSYSRT
                     I"
     gene            complement(12604..>13575)
                     /locus_tag="ACHU66_001307"
     CDS             complement(12604..>13575)
                     /locus_tag="ACHU66_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR3389588.1"
                     /translation="NVEQYLSKCINSIVNQTYKHIEILLVNDGSTDNSEEICLAYAKK
                     DSRIRYFKKENGGLSDARNYGISRAKGDYLAFIDSDDFIHSEFIQRLHEAIERENALV
                     AVAGYDRVDASGHFLTAEPLPTNQAVLSGRNVCKKLLEADGHRFVVACNKLYKKELFE
                     DFRFEKGKIHEDEYFTYRLLYELEKVAIVKECLYYYVDRENSITTSSMTDHRFHCLLE
                     FQNERMDFYESRGDKELLLECYRSFLAFAVLFLGKYNHWLSKQQKKLLQTLFRIVYKQ
                     LKQNKRLALLMNAYYLVGCLHLNFSVFLKTGKDKIQERLRRSESSTR"
BASE COUNT         4182 a         3004 c         2382 g         4007 t
ORIGIN      
        1 tggttgaaat acccactttc attttcttct taaccatcat ttcaggagaa aatttttgct
       61 tagtatagtg taaaattttc tctttcaatt ctttcgttag gattatcttt tttaccgatc
      121 gttttcgatt agtttggtat accgtttgtg catagtcagc agaatagact tttttgtaca
      181 gcccttttcg gacttgttgt aaagttgtcc cacgtttgac ttcattatag aactttcata
      241 agtagcttag acttgctgtt tttgattttt attgatactc ttcgaaaatc aaaattatcc
      301 gttgtcaact tgcctttatg aactccaact ccagttctat cttcggcttc gtttcctagg
      361 ctactttgta accgcccaat aataaggtat attgaaaagg ctccaaagtc ctgttgactc
      421 tggaaccttt ttctatttac atgctctctt aattttcttc tcccatggca aataagcctc
      481 tagaacttct tttttagcga gtgtttcctc gttaggtaga ttgtctagaa gataggtcat
      541 atatttctct gtatcaagtt cgtgcctctt ggccgtttcc aaaagactca aaatgatagc
      601 tgtcgacttg gctccctcaa aactttggga gaacaaccaa ttttttctgc ccatcaccaa
      661 ggtcttcata gccctttcag ccacgttgtt agacaggacc aagtcaccat ccgagagaac
      721 ggttcggaag gtggtttcgt atttgaggct atactccaaa gcagtaccca atttggagcc
      781 tggcaaaaca gattgctcac gacaccagtc aaagaactcg tccatcaagg gagccagcgt
      841 ttatgcagtc gctcctcgct agacaagtca gcccagtcgc tctccaaggc aaacaggcgg
      901 tcgcaatagg ctaagccctt ggctcctaaa gaagtcttgt ctgtcttctt aggagtcgcc
      961 tcaaagaact ttcgtttgac gggagcccaa caaccaacca gctgagcctt gtctaattga
     1021 cgataagcac tccacatgtc acaatgtacg tagcccgcat agtccccaag aacctcctcc
     1081 acaaccaagc cactccgtcg tttgtcatga tgatagaggg taattccatt ttcctcatgt
     1141 ttcccagaca agaaggtcca gtagaaggtc aactgaccat cattttctaa gaccttgtag
     1201 gcggtctcat ccgcatgaag aataggctgc tccaacaatt tctcgtgcaa caggttgtaa
     1261 atcggctcga aataatactg actagacttg atatgccagt tggctatttc cttccgactg
     1321 atgggcaggc caagcttatg ccaatcctct tcctgacggt aatttggcac cttcaaattg
     1381 aacttctgat gaatggtgtg agcgatgata gaggctgaac ccaagctgtg tgccaaaggt
     1441 gccttaggaa cgggagcctt gataatctta tcgctcagat tcttctggct acatgcctga
     1501 tacttgtatg cgtgttgaac atggtcaatc cgctttaatt gtgcaggaat gaagaccaac
     1561 tcttgccgtt gaacagctga gccaatctct ttcaactgac catgacagtc tggacaagtg
     1621 cagtcttcgc cctgcaattc gtgatgcact atctctggag tgaactgact aaaaatagcc
     1681 tgacgaactc ccttagtttt cttgcgttta taagtgatga tctctgtttc aactgggtaa
     1741 gtcagcttct tcttcaagga ggctttcctc cccaaacaag ctcagctgac cgggttgata
     1801 cacaactttc tctgatgatt ttccgtagag tttctgtctc agatagtcaa cctgttctcg
     1861 aaggagggtt agctcattgg taagttgttc aatcgttgaa gtctgttgtt taataattgc
     1921 caacaactca tccatacgct cgctcctcct tctttttatc tcattataca caaagaaaag
     1981 ccatgatttc aatagaaatc acgacttttt gaaacattta tttttggaat gatagaaaat
     2041 cctttcatga gctaatctac ttgttcggaa gtgagggact taacctcctc ttcattgttt
     2101 ggccaggtca atttcccatt ttcaaaacgt ttatacaata accaaaatcc ctgtccatcc
     2161 cagtaaagag ctttgaatcg gtcttttcga cctccgcaga agagatagac ctgacctgaa
     2221 aagggatcca gattaaattg acttttgaca agataggcga gagagtcaat cccctgacgc
     2281 atatccgttt tcccacagac caagaagact tgacctaaat cactgagtcg gatggtcata
     2341 atgcagcacc ttatccaaga ttgtttccat tacttcaggg ttgatagatt ggaagaatgt
     2401 gacttccgtt tttccgagac gaattttcat cagaatatcg tttctgcttt tcttttcaaa
     2461 acggcgagat tggggaatag tcaattggac gacgggctgt gacataatga atcctccagt
     2521 ttgtttttta taacagtata atggaggaat gtgtgattgg atagatacct tgttattagg
     2581 cggttacgct ttaaacatca aaaatgagag aacgaatttc gtcctctcta ggtgttagct
     2641 tttcgtctac ccactacagt tgacaaagag ccgttttttt attgtgagaa actagttttt
     2701 gcccagcctc tttttgctta ttttcctatt tttgggcgca ctaagcccac gatggtaatc
     2761 ccagtcgttt gatttgctca aaacgacaga tattgtagag caggttggtt aaggtcacat
     2821 tggttttagc tcgtgccttc ccaattgctc gacagatgtt acctttcatg ttagtttcaa
     2881 tgaagccaaa accatgctca acgcgacaac ggactttggt aatatgtcga ttaatgacct
     2941 tatcagtctc agtcaacggt ttatttctaa aagcacgacg aatcgtgtgg tggcgacaac
     3001 cttctggtac tgattttcca acataagcac tgtcatcaaa aaccgcttca ttggcatcac
     3061 aaagaggagc taggacattg gagtcgtgta cactagctgt tgtgacctca taattttcga
     3121 taagttttga ttttctgatt gtcctcacgt gaattgcgtt gtttagggca ttcgacaaag
     3181 gtagcatcca caatctgacc tgaatgggca atcacccctt catctgtgag atgggcatag
     3241 aacaaatcga acaactcctt ttcacgacct gacttggtta atttctcacg atagagccag
     3301 atagttttcg catcgggaac agtgtcttca tgacaaccaa caaaacgacg aaaagatata
     3361 cgatccagca gttgatattc catggcatcg tcagataggt tatgaagacg ttgaagcaag
     3421 agcgctttga acatcatgag atagtctagg tgaggacgac cgccacgact gatgacttta
     3481 tctttacgac tgaataactc tgacaataat ggaagaaaga tattccaatc cataacggca
     3541 tccaaacgtt ctaagggatt gcctttctct gatagttttg acaagatttt ttcatcgtct
     3601 gtgaataaat gcatgacagc tctcctcatt actttttatt ctattatctc atttttggga
     3661 aatttaggga gtttttagag gtccccttat ataattaaac aactaaatgg ttgtcaattc
     3721 tcaaggctag catcctctac agatgttatt ccttggctct aaaacacata ccaacatagt
     3781 ctagccactg aaatagcttc tatcaccaaa ttatcttgtc aaacttgtca aaatcagctg
     3841 attcacacgc tcttgtccaa tctgattgaa atgcaatcca tcattggtat actgatagtc
     3901 aagcatcgcc tctttttcaa caacttcatt tagatcaatc actgaaatac ccagttcact
     3961 tgctgactga acaataacgt cattcagctg cacaatttct tcatttgaaa cgctatcacg
     4021 aaacagcgtg taggcaatcg tcgtcacaaa aacctgctct gctttggaaa caagactttc
     4081 catcagctgg cgaatagtat cctcaatcat atgcaaggga tggtcagtaa tcaaatcatt
     4141 cacaccaaga gaaagcaaaa ctttctttat cggagtagcc aaaaagagac cttggttttc
     4201 aagtgctgtc gaagctgtca tcccaccgat gctgatatta tcgaaaccat ccagtgacaa
     4261 ggctaacaga cgactatcgc caatcacaag actatcatgg actcgttgct cacataaacg
     4321 ctttaactct cttttataaa atggtttgtt ttgttgctca cgacgctggt aatcaaagta
     4381 aattcgacca ataacaccag taaaatcaaa gtgatcatct acatcaatcg aatcttcctt
     4441 cgtcatcaca taggccgctg ttttttcaga aaaataagtt ttatccgcta aataagcctg
     4501 cttagaagaa atataaatcg ctccattagg atagtagagt gttttctcat cttgacgacg
     4561 ataactgcca cctaatcctg caatatcctt agcgaatccg ttttcgtcta aagttgaaaa
     4621 caatgttgga gacttatcga ctttggtaaa gctaacaacg tggtcagctt gacctttccc
     4681 atataactcc atcgcctcct tgacatgttt tcccgatctt aatggggacg taacttgcag
     4741 gagaacaaat acttggtcat cagaaaaatc ttgtaaaaaa tgttcgttca gttgaaaaga
     4801 ggttgtaaaa tctgtcgcca agtcagctgg acgcatgagg acttgaaccc cagttgtttc
     4861 acaaatttcc ttgtaaacct ctgaatcagt actgacatat atattttctt tcttaaaaca
     4921 tccagactca atcgcagctc gaatggtatg gaaaatcatc ggtacaccat ctaaaaataa
     4981 catgttttta tttggtaaac cttttgatcc tgaccgagca ggaatcagac aaattggttc
     5041 catcaattca cctatttaat ctttctagca ggtacaccaa cataggtccc tgactccgtc
     5101 aacgatttca aaacaactgt ccctgccccc aatgttgtat aaggtgcaat ctcgatacat
     5161 tgaatcactg ttgaaccact tccaatataa gtgccttctc cgatacggca caagccattt
     5221 atggtcactc ctggagtaat gttacaatgg gcctccaccg tggtatgatg ttccacaatg
     5281 gcacccgtat tgatgataca attgtcatag acataggaat cggctcctac aaaacttgaa
     5341 aaacctatga aaacccctcg tcccttgata ctatctgggg aaaaaatatt ggcttgctcg
     5401 ctaatgatgt tgaacaaagc atcataatga tctttggcaa gcaagtcaaa gatttccttg
     5461 cgcttgacat tgtcacctat agtgacgaag acagcatcta cttttccatc atccaaatag
     5521 gttaggacat cttgcaaggg accaaataca ggatagccac gatagtcact gatcggttta
     5581 tcttcaaaat atccaatgag ttcatatcga cttctatcca accaaggaag gacaccatct
     5641 gaaaaggtac ccgctcctag aaaggctact tttttcatca aactttctcc cctatctatc
     5701 ataaaactct ttcatggttg aggcctgtac agataaaaat tccttgatag ctcgataacc
     5761 ttgtaaagca gaatcaggtt gttcaaatgg attggtaaaa tctatcacat cacgtaattg
     5821 ccccaaacca ccaacaatcg cttccttaga agttccaaca tgtaccacac tcggtcctga
     5881 caaacgtcca aattggcgat ttccaatatt taaggttgga acctgtaatg agggcacttc
     5941 aatcaaacct gacgaagaat tccctattaa accttgtgaa tgcttgacca aggaatggta
     6001 ataacgagtt ggaagcgaag taaagatgta agaatcagag tcttgtttta caaattcatg
     6061 catcaattcc attatcttat cggcatgtgt atccgaattg gatccaatta tcaaacactg
     6121 gctaccatct tcttttagag catctaataa ggcctgcgtt tgttcttcgg ctgtgttatc
     6181 ctccaaggta acagggtgaa agagtacaac atagtaatcc tcggcaaaat caattccaag
     6241 ttccatcgcc aactcttctc ttgtcaaaaa gtcttgtttt aaaacatttt caacacccat
     6301 agctccgatg ttcagtacat ggttgggatt ttctcctagt tgaatgacac gatttctaaa
     6361 ttcatccgtt gatgtcagat gaaggtgact catcttggta atggcatggc gaatcgactc
     6421 atcaaaattt cccatggttt tttcaccacc atgaatatgg caaataggaa tattataaag
     6481 caacgcagca ttggcaactg gtagcatctc atagcgatcc cccagaatca acaccaagtc
     6541 atactggact tcttcaaaaa gaaccgtgag ttgctctgtc aaggtcgcta aagatttgac
     6601 gattgtctgc ttagacgtat ccgtcaaatg caatggaatc cgcttgacaa tcctacgctt
     6661 gtccgcttcg atgtctttga ccgtcatccc atatttttct tctagatgca tggctgtcac
     6721 tacaagatcc agctccattt ctggatcatc ctgtagatag ctcaataagc gacgcataat
     6781 cccatattcg gcacgagagc ctgtcacaaa acaaattttt ttcatcctta ctccgcttac
     6841 atttgatttt caaaagcact atggcaaata ttttggtctt cctcaaaatc ctgctcactc
     6901 acctgcccca agactttgta ccattccatt ggcgaaattc catttcctgg tcttttgaca
     6961 gtgatgtttt cttctgtaaa gacttcgcct ttagcaattg cttttttggc aacaatagat
     7021 tttctagcta caattttatt tcgtacttca acttcttctg gctctttttc aaatttacca
     7081 agagattgtt ccactatcct cactcctttt accaaggctg ctaagatatc aggagtagca
     7141 ctcgctttat gatctggtcc ttccatttca ttgtccagag taaagtgctt ttcaatcaat
     7201 tcagctccca ttgctgcagc agcgatgggt acttctgaac caacactatg gtctgaatag
     7261 ccaattgtta agtttggaaa ttcttttttc aaggtatgca agacattcaa attcaaagca
     7321 gggtaagggg ttggatactc ggttgtacaa tgcaaaatcg aaatatcggt cgttccattt
     7381 tcctgcaaaa tcttcaccgc ttgatgaatt tcatccataa cagccatacc agttgaaaga
     7441 ataactttct tagcttgacg accaattttt tccaaatagg gaagattggt aatctcacca
     7501 gatggaatct tataaacggg catatctgtg ctaatcaaga agtccaatga ttcctcatca
     7561 aaaggtgtcg aaaacacatc aactcccttt tcaagacagt aatcacgcaa atcaagatac
     7621 tcttcaaagc tcaattccaa acgacgagtc atttcgagct gagaatctga ctctcctgtt
     7681 gtaatttttt ggtattctgc ctttggtgcg tattttgaaa tcaacaaatc tgccttaaat
     7741 gtctgaaatt taacggcatc cacaccacaa tcaacggcaa cttctaccat tttccgtgct
     7801 agatgaacat caccgttgtg attacaacca atttctgcaa taatataaac cattttattt
     7861 cctttctaaa aatgactgta aatcgctccc ataaagtctt gagggagcag cttggttttc
     7921 cttaagtagt aaaagaacac taataaaaat cgctacttca cttctgatag ccgattagaa
     7981 aaaatagccc tagatttctt tcgaatagtc tcagggcttg ttgcttctaa agcctgcttc
     8041 ttcacacgat cttcaataga ttggtggaag tttaatctgc ttttctaccc aatttttagg
     8101 gagatttacc tcatgacttc tggcataagc agaggcgcat acgtccttta agaaacattc
     8161 ccttctttat acttgattat agcacaattt tcatggtaga gaatacttga actttgggta
     8221 gatggcagtg atggtcaatt cttatgagca cctctcattt aggacgtttt tatatgggga
     8281 cctctaaaaa ctaattttct aaaatttaaa atgttgattt aacaaggctt tcgtaactcc
     8341 aaattcgcaa ttttgggagt ttatagaggt gcccttattt agaccgtttt tccctgaatg
     8401 tattgagggc aactgttaat tcctttctaa aaatgtaggc ataaacgact agaaccgcta
     8461 ttcctagtga ccaacgaatc cagattgaac cgacaaatac tgtcatcaag cctgtataga
     8521 cgacaacaag agcaattacc ttaacaaatg ttgaaatcgc aacttcatcg taagcatact
     8581 ttttcttagc aacaaaataa tgcaagacta gcaacaacag ataggaagca gtcgttgcca
     8641 aagcagcacc ataaattcct atcgtcggta tcaaaacaaa gttgactgaa atatttagta
     8701 cacctgctat aaaagtacca attggcaaaa actttgtatt tccactataa aactggatat
     8761 tggctggaaa actataaaga aatacaaaga acaccccgac aataatcatg ggaataaatc
     8821 ccatactgaa acgatactca gatccaccta acaacatcgc taattcaggg taaattgtta
     8881 gaaatccaaa agtcacaaac aggccaatcg ccagatagta actgacataa ctgagcaaat
     8941 ctttatctgc acctctcttt ttctcaaaat accacggaca ccataccgta ttcaagctcg
     9001 aaaacacaat ttgtaagata gacgcaagtg tgtagccgaa actgtatagg gctacatctg
     9061 acagtgttag catcttgccg agcatgattc tgtcaaattg attgagtaca ttatgaccta
     9121 atccatgaaa aataagagga atcgatatac ttaaaccata ccgaagatag tcctttcgaa
     9181 aatgaagccc aatcttctta tagaaaaaca acaaggacac acaagcaaaa acaccagtcg
     9241 ttgccgagtt tgccattaca cgagcgatga aatcattctc catcgaaaag atgagaaata
     9301 aagataaagc agtgttaata acagcgctca gtaccgatag gagtaaagtc cacatggact
     9361 gctgccgctg cactaaatag gtcgtaaaaa atccttgcac aacactcata aaactttgca
     9421 aaaagataag cggaacgacc caatcaggca aaccaaatag gagcgatagg ggctgactga
     9481 ggagaaaaga tagcccaaaa attggtaaaa agaaagcgat agaagagacc atcaaggtgg
     9541 atacgaaatc atcaaatttc tcgcggaagt gtacccatcc cgggccaaaa gccccaccta
     9601 actgtagacc gataaagaga ccaactagcc ccacccacga attatacaag ctaaactgcc
     9661 catatacttc ctgagaaatg acacgagtat agatggggag ggtaatgaag gctaatccct
     9721 gaacgaggat ggttccaatc gtatagacga gacctgagac aactattgat tttttagaca
     9781 tgaatcctcc tattctatcc cctcacgcaa aatatcattt tttgaaagaa gaggaaaagt
     9841 gtcctttaaa tacactttct cttcaaaaca atttaaaaaa tctaaagcag acgaactata
     9901 ggtcataccg atatcaaaac ggatactacc tgccatttcg aacaactcaa acggaatacc
     9961 ttgaggcaga agcaccacag ccttacccag aaatgaataa tcaaccccat ctcgtgggtg
    10021 cggttttatg tagattgtat agtcttctcg ataaggggca agacctgcta cataaatctc
    10081 caacaactcc tcttcggtca catgataatc ccaagacaag ggctgggtca ataatagcaa
    10141 tcgtttttga gaagactcct catccgctac tatcgccctt gctccaaata tctgcagcag
    10201 cgcttgcacc ttctctggcg atgcttgatc aaataattgc tttctcggaa cttctacaaa
    10261 gggtttataa gcctcgtcaa attgtacgag cgacagatca ttgacctcaa tggattgaca
    10321 atagagactt gaaccaatca aatatgttgg tttaaaatac cattgataaa agagtctttt
    10381 ccagacagat gattgaattg actccaaatc gcacactctt ttatccttga aaaaattata
    10441 accatcctcc aaaagagaat agggaatccg atgtttattt aaaaaatgac cgacttgacg
    10501 gtgatcttga aaaatgatca cctcgtcata gctctgtaac aaggaaaggc gttcttttgt
    10561 ttttgatcta gctatcaaag aatatagatc aaatgaacgc tccgtctcct cgattagatg
    10621 aacatgctgc tgcagttgct ccctgacatc tggaaaatgc ccgatgatat caacggacat
    10681 caaactatct ctctcaacgt ccatctttaa caaggaaatc aggatctgat acatcgtatg
    10741 gcaaatataa attcgtctca atttttctcc ttctacatac agactttaag gataagtaac
    10801 ccctttttat aacccctgta cagcttcgat aatgtactga ttcactcttt cttgcccttc
    10861 atcattgaaa tgcaaaacat tttctgagtc cccctgtaag tacatattaa tggcttcttt
    10921 tatctgttcg ccagtccgta acggtgaggt gacttgcaga agaacaaata tacaatcttc
    10981 ataaaatcca gattcgtaaa aaagtgcgta ctgacttcat tcgatggagt atgataagta
    11041 gatagttctt tgggtcttaa caatacattg atgcctcttg tctcgcaaat ccccttgggg
    11101 acctctaaaa actaattttc taaaatttaa gatgttgatt taacaaggct ttcgtaactc
    11161 caaattcgca attttgggaa tttatagagg tgcccccctt atacatttct gaatccgtac
    11221 tgacatagat gtcttctttc tcaaaacaac ctgattcaat tgccacatca atcgtgtgaa
    11281 aaatcatggg tttcccgtcc aaaaatagca tgtttttatt cgataagcct ttcgaaccag
    11341 atcgtgctgg gattaaacaa acagttctct taaaaattct ccttgttcag ttaataaata
    11401 tagctttgaa aataaagggg gatactgggg tttagaaaca gataccactc ctctctcgtc
    11461 aacctatttg aatttctttg tcggaatatt aacatgcgaa tctgctaata aacgaaatgg
    11521 catctgctaa tctgaaatga gtcattatcg ttttccacgt actctcataa acagatccac
    11581 tttgtagaaa atatagccac caaacaggca aattagaaag gtaaacaagc cgttccacaa
    11641 attcaacgaa gataccttaa attgcactgc taaaaatggt gcgactactg aaaaatacag
    11701 catctgggtc aagtagatat gataagaagc aactcccaat tttgacaaca gtgttgctgg
    11761 aatcttctgt ccatacttta tcataaaaaa tagcatagca tacgcaaatg ggacgcatag
    11821 aaaggaagta gacttccaac cataaaacca ggagaagggc tctatagaat gattcacaaa
    11881 aatcagaatt gctccaataa tcccaaatag ggtcgcaatg aaagtatcta ccttggaacg
    11941 cacatcctgc tttgaaagaa aaaaccaagc cctagaacaa aaagataacg aagtgatatt
    12001 agtctatata tatatgccgt gttcagccat gttagcaaat atggcatcca ctgaaaagtt
    12061 tactaaaaaa caagtaagca agcccaacca tttatttttc tcgaaaagaa catacaaaat
    12121 tggtaataaa aaaactacct gaatcaacaa cggaacatag taactcccaa ctccctggac
    12181 ccgattctcc agaaagtagc cacaggacaa atgaacggag cgatgaagaa cctgaaaaag
    12241 gctttactcc tatcagatgg ttctcgaata gctcaataaa acattaacag ccatcacgat
    12301 agcatagaga caaagcatgt ttatactttc ttttatacca ctgctgaact tgagctttag
    12361 cgtctcttgt tttgtattcc acttattcgt tcgaaaatag gcagaaagca acagaaaaat
    12421 tggaacagcc atgtcaacga aatacggaaa gataaaccat tgacgctgct caacagacca
    12481 atccagatgt gtaaaaataa cacaaagtac catgatacat ttaatcgtat caaagagtgc
    12541 tattctacta tttgttggat tcattttaat ccccttttct ttcaaccatt tattacaaca
    12601 ttcttaccga gtactacttt cacttcttct caatctttct tgaattttat ctttccccgt
    12661 tttcagaaag acactaaaat taagatgaag acaccctacc aaataataag cattcattag
    12721 taaagcaagt cgcttatttt gcttcaattg tttatataca attctaaata gcgtttggag
    12781 aagcttcttt tgctgtttgc tcaaccaatg attatatttg cctaaaaaca aaacagcaaa
    12841 ggctaaaaat gaacgataac actctagtaa gagctcttta tctcctctac tttcatagaa
    12901 gtccattcgt tcattttgaa attccagtag gcaatggaag cgatggtcag tcatgctaga
    12961 agttgtgata ctattttctc ggtcaacata atagtacaag cactccttaa ctattgcaac
    13021 tttttctaac tcatagagca agcgataagt gaagtattca tcttcatgaa tcttaccctt
    13081 ttcaaatcga aaatcttcaa atagttcttt tttatagagt ttattacagg ccaccacaaa
    13141 gcgatgacca tccgcctcta gcagcttttt acaaacattc ctgccgctca gaacagcctg
    13201 atttgtagga agcggctctg ctgttaagaa atgccccgaa gcatctaccc tatcataacc
    13261 agcaactgcc acaagggcat tctctctctc aattgcttcg tgtaaacgtt ggatgaactc
    13321 cgaatgaata aaatcatctg agtctataaa agctaagtag tcacccttgg cgcgacttat
    13381 gccataatta cgggcatctg atagcccgcc gttctctttt ttaaaataac gaatgcgact
    13441 atctttcttc gcatatgcta aacaaatttc ttccgaatta tccgtactac cgtcattcac
    13501 cagaagaatc tctatatgtt tgtaggtctg atttacaatg ctatttatac acttggatag
    13561 atattgttca acatt
//
LOCUS       DAZQCJ010000051        13214 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000051 DAZQCJ010000000
VERSION     DAZQCJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13214
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.052"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(116..454)
                     /locus_tag="ACHU66_001308"
     CDS             complement(116..454)
                     /locus_tag="ACHU66_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HFR3389589.1"
                     /translation="MSTNIYDIANELERAIRNLPEYKAVEAVKVSVEGNSEAKEILES
                     YISFQKEIQSKLQAGEIPTEADQKKMLDFNKKVQGNPLLTEYFSKQQQLGTYVADLER
                     IIFKPLNELL"
     gene            complement(464..1567)
                     /locus_tag="ACHU66_001309"
     CDS             complement(464..1567)
                     /locus_tag="ACHU66_001309"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFR3389590.1"
                     /translation="MKKTILIVGLGLIGSSLALCIRKDHPDYHLIGFDPNEQSCQIAL
                     GKGMVDEVSTELEGVAKQADVILLCVPIQVSLRLIEEFSALELKDTVLITDAGSTKSA
                     IVAAAEEHLAPRQINFIGGHPMAGSHKSGASAADLHLFENAYYIMTPCQATKQDAVPR
                     LTDLLSGTGARFVQIDAAEHDRVTSQISHFPHVLASSLMHQAGEYAQEHSFTNNFAAG
                     GFRDMTRIAESEPSMWTSILLTNADSILNRIEEFQERLSEISDVLRSGDQKAIWDFFD
                     KGRQTRRAMEIHKRAGVDSFYDLFLSVPDEEDTILKVLEVLRGISIVNIRINEENRED
                     IHGILQITFKNKGNLEDAAKRLREQTTYKIHLD"
     gene            complement(1575..2741)
                     /gene="aroC"
                     /locus_tag="ACHU66_001310"
     CDS             complement(1575..2741)
                     /gene="aroC"
                     /locus_tag="ACHU66_001310"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR3389591.1"
                     /translation="MRYLTAGESHGPRLTAIIEGVPAGLPLTAEDINEELKRRQGGYG
                     RGSRMQIETDRVEITAGVRHGKTTGAPITLNVTNKDHQKWLDIMAVEDIEEKLKSKRR
                     IKHPRPGHADLVGGMKYRFDDLRNSLERSSARETTMRVAVGAVAKRILAELDIEIANH
                     VVVFGGKEIDVPDGLSVAEIKERAAQSEVSIVNQEREEEIKAYIDQIKRDGDTIGGVI
                     ETVVGGVPAGLGSYVQWDKKLDAKLAQAVVSINAFKGVEFGVGFQAGYLKGSQVMDEI
                     LWSQEKGYTRRTNNLGGFEGGMTNGEALIIRGVMKPIPTLYKPLMSVDIDTHEPYKAT
                     VERSDPTALPAAGVVMESVVATTLATEILEKFSSDNMEELKAAVASHRDYVKNF"
     gene            complement(2745..3419)
                     /gene="aroD"
                     /locus_tag="ACHU66_001311"
     CDS             complement(2745..3419)
                     /gene="aroD"
                     /locus_tag="ACHU66_001311"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR3389592.1"
                     /translation="MKIVVPIMPRNLEEVETIDVERLAEADIVEWRADYLPKDDILRV
                     APAIFEKCSGKEVVFTIRTTREGGHLDLDDQEYVNVIKEVATLYQPDYIDFEYYSYKS
                     VFEQMLEFPNLVLSYHNFEETPSNYMEIMSELTSLSPAVVKMAVMAKTEQDVLDVMNY
                     TRGFKSLNTEQIFATIAMGELGKLTRIAGVITGSCWTFASLDETSAPGQMSLSNTRKF
                     LEILEN"
     gene            complement(3416..4579)
                     /locus_tag="ACHU66_001312"
     CDS             complement(3416..4579)
                     /locus_tag="ACHU66_001312"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFR3389593.1"
                     /translation="MKIKVNRIAEQKINRGIQLLDSADFSGVSFDEDRLAVLYSESGS
                     YLGQAYLSKQNKGIGWLFSRSNQDLTMDFFVKLFVDAKQKRQHFYQSEMTNAFRLFNQ
                     DGDSFGGMTVDLYDEYAVFSWYNTFIYSVKEMIVAAFQQVFPEVRGAYEKIRFKGLAF
                     ESAHLYGEEAPEYFTVLENGVHYQVFMNDGLMTGIFLDQHEVRGSLVDGLAAGKSLLN
                     MFSYTAAFSIAAAMGGAEETTSVDLAKRSRELSKAHFKVNSLDLDNHHFVVMDVFEYF
                     KYAKRKGLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNTGGTIIASTNAA
                     NVSVEKFKRQIEKGFDVRKHRYEATYRLPADFVINKNDESSNYLKVFTIQVDK"
     gene            4695..6869
                     /locus_tag="ACHU66_001313"
     CDS             4695..6869
                     /locus_tag="ACHU66_001313"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFR3389594.1"
                     /translation="MKKLIDYVKKSGQTRLGFVLILLIIYWLKTLWAYYVDFNLDTQG
                     FFQNFIAIMNPLPVGILLLGLSLYIKNTKVFYSVAMIIYIILSVWLFSNAVYYREFSD
                     YISISTIMATSSVSAGLGEAALKLFRLWDIVYFLDFIIFGILVYRKLLRWDKKTFHKK
                     SSFAITALSVMLFSINLFLAEIDRPELLTRGFSNTYIVRALGLPAFLGYNANQTYNAH
                     KNRSGASATDLEPVKQYITEHYAQPNDNYFGIAKGRNVIYLHLESLQQFVIDYKLNVN
                     GVEYEVTPFLNSLYHSSSTLAFSNFFNQVKAGKTSDAETMIETSLFGLNQGSFMVQYG
                     GSNTQQAAPYILSQNGGYTSAVFHGNAASFWNRNNTYKQWGYNYFFDQSYFSKATAEN
                     SFQYGLNDKIMFADSIQYLEHLQQPFYTKFITVSNHYPYTTSLIGDEIGFPLANTDDE
                     TINGYFATANYLDASIKAFFDYLKATGLYENSVIVLYGDHYGISNSRNPDLAPLIGKD
                     SQTWSDFDNAMMQRVPYMIVVPGMTNGGIFDTYGGEIDALPTLEHLLGIKSDNYLQVG
                     QDLLSSQHSQIVALRTAGSFITPKYTSYEGKIYYTETGLEITNPDETTQKEIDAIKEA
                     TAAQLATSDAIQTGDLIRFFDNGLPALDPSQYNYIDSLAAEIALENKLGKQSTSLYST
                     NGNKSTIELFKAPSYLELHGSTTATSTPNTGTSSTSTSSNSR"
     gene            complement(7166..9670)
                     /locus_tag="ACHU66_001314"
     CDS             complement(7166..9670)
                     /locus_tag="ACHU66_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HFR3389595.1"
                     /translation="MKQGKTLLYVAGVSLICAVALVTYQASNYREQSNHQKTTSIETS
                     QSKQSKEIPSDSKKEVPGIDKATDDGFLLTDESQIEEKTDLGIIVNHGDHKHFFFYSD
                     LKNTKWAYLIPENYQEKNHSSQPKRSQLSSGSKQIEDEYVFDPKDIVAEDANGYTVRH
                     GDHYHYILKSSLGGTTHRQLTSDSRQSPTLPIVHQQEGIPGIDFQTSDGFLFDGQNIS
                     GVTETGILVRHGKHLHLIHFETLKRSKWSYLVNQYKPNVESDKKAQPEVENTEYQTKL
                     DYLAKELNLEPSRFKKVIVDGQIGLEYPHGDHTHIVLLKEIDTTKPFESPEDRILKQK
                     DGETLEQRKERLIKQYMERFKVKREDITIDGNYMSVRHGDHAHVYKIDPSLPDDPERD
                     VKTETVNLAIEKQLVYGPFYTEGSTENLTRNGVHQKYHPEGLQDIKNFILVTFSTNSD
                     FGDFVVNGKKTKRVYYLVRKDLNWEDLNIAYPQTLQQKGRVFNGWNATLPKSGKMARE
                     HQSFYADFDNVYRKPTKNIYTPSDDVSNIDLSDYVPVKYSAIANGRLKLNGEIRAGFI
                     YFVKSDLTWKQAKEQGLVVPEPVPSKDYEFIEFRTVITGGEKDTDYVSATTSLAAFGT
                     TAPRIGPYIAENMDNPTDINDPSRHPNYYWHDPKNYVALAFRAGDGGQLQTHLGTSKT
                     VVYLVRKGLTLNQAGIFPPSLKSDTGYKRDYTKPVIPNVAWDTPILEDTVYDIHFDKV
                     ESDSKDGTVEPGDTWLPGNPLPNTPDVPNGEEDADSWLDDLLTPSPEATDIETVTEAE
                     TTTEDTKATESSTAAPIDIPKKSSTEEEPSEDFIFP"
     gene            complement(9996..10355)
                     /gene="rplT"
                     /locus_tag="ACHU66_001315"
     CDS             complement(9996..10355)
                     /gene="rplT"
                     /locus_tag="ACHU66_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR3389596.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHLLFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AAAFTALADAAKAKLAK"
     gene            complement(10399..10599)
                     /gene="rpmI"
                     /locus_tag="ACHU66_001316"
     CDS             complement(10399..10599)
                     /gene="rpmI"
                     /locus_tag="ACHU66_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR3389597.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKAGMVHAGDFKRIKSMLTGLK"
     gene            complement(10626..11147)
                     /gene="infC"
                     /locus_tag="ACHU66_001317"
     CDS             complement(10626..11147)
                     /gene="infC"
                     /locus_tag="ACHU66_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR3389598.1"
                     /translation="MAKQDLFINDEIRVREVRLIGLEGEQLGIKPLNEAQAIADSANV
                     DLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGDFET
                     KLRHARKFLEKGNKVKVSIRFKGRMITHKEVGAKVLAEFAEATQDIAIIEQRAKMDGR
                     QMFMQLAPASDKK"
     misc_feature    complement(11173..11301)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(11328..12005)
                     /gene="cmk"
                     /locus_tag="ACHU66_001318"
     CDS             complement(11328..12005)
                     /gene="cmk"
                     /locus_tag="ACHU66_001318"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR3389599.1"
                     /translation="MKSIQIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYL
                     ALTNGIEVTDQNRIVDLLAQYPIRFGRDENGQQLVFVGDEDVTLPIRDNQVTNNVSAV
                     AALPLVREELVRLQQDIAQAGGIVMDGRDIGTVVLPQAELKIFLVASVEERALRRFKE
                     NTERGIETDLESLKEEIAARDYKDSNREVSPLKAADDAITFDTTGVSIEGVVKFISEK
                     AKEILDR"
     gene            complement(12015..12491)
                     /locus_tag="ACHU66_001319"
     CDS             complement(12015..12491)
                     /locus_tag="ACHU66_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFR3389600.1"
                     /translation="MSQEPWNEEVYHTETTSRKERLSKGVADTTVFTVLAIIFFVIVL
                     IIGIMAVYLSIGGGSKTDSTKEFYTPSTTTVVAESSSSAAETQESTSTDTTETETSSS
                     PAEATDGSTLTVQAGEGVGQLAARGGISIAELERLNPEKMTTGSWIAHPGDVVRIR"
     gene            12542..12736
                     /locus_tag="ACHU66_001320"
     CDS             12542..12736
                     /locus_tag="ACHU66_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFR3389601.1"
                     /translation="MKVKLIPERCIACGLCQTYSSLFDYDDDGIVVFSDESQFEKTIE
                     PNPDILKAIKSCPTKAISAD"
     gene            complement(12725..13213)
                     /locus_tag="ACHU66_001321"
     CDS             complement(12725..13213)
                     /locus_tag="ACHU66_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFR3389602.1"
                     /translation="MIKIFGKIRYHWQPDLSWAIIYWSLTFTPIFIGMTLLLEKLQVS
                     RLFILLVSLFAVLVVLGLHRYFEIKDEHLRIASANPFAVQKIEIATIEKIEVSYLAIR
                     IFSQEFPNGQVYHMRKWTKKYFINHLAIHSCFKGKIELIDHLIKQDYFEEYYTKKAKS
                     IR"
BASE COUNT         3852 a         2939 c         2264 g         4159 t
ORIGIN      
        1 tactccccaa gtaccgtttc tgaatctgta cttggagata agaactctac gtaagcagcg
       61 tatactaggc gtcaatccat gacctaacac cctgctccta tcaaatataa atatattaca
      121 acaattcgtt gagaggctta aagatgatac gttccaaatc agctacataa gttcccaact
      181 gttgttgctt gctgaaatat tccgtcaaaa gtggatttcc ctgaactttc ttgttgaaat
      241 ccaacatttt cttttggtca gcttcagtag gaatttcacc agcctgcaat ttagattgga
      301 tttctttttg gaaagaaata taactttcca atatttcttt ggcttctgaa ttcccctcaa
      361 ctgagacctt aacggcttcc accgccttat attcaggcag gttacggatt gcacgttcca
      421 attcatttgc gatatcatag atatttgttg acatattttt ctcctaatct aaatggattt
      481 tgtaagtcgt ctgctctctt aaacgtttcg cagcgtcttc caaatttccc ttgtttttaa
      541 acgtgatttg taaaataccg tgtatatctt cacggttctc ttcattgata cgaatattga
      601 caatggaaat accccgtaag acttccaaaa ccttcaaaat cgtatcttcc tcatcaggaa
      661 cactaaggaa taaatcgtag aaagaatcaa cacctgcacg cttgtgaatt tccatggctc
      721 tacgagtttg acgtccctta tcaaaaaagt cccaaattgc tttttgatcg ccagatctga
      781 gcacatctga aatttctgaa agacgttctt gaaattcctc gattcgattt agaatagaat
      841 ctgcattggt tagcaaaatg ctagtccaca tgctcggctc actttccgca atccgtgtca
      901 tatctctaaa tcctccagct gcaaaattat tcgtaaagga atgttcttgg gcatactcac
      961 cagcttgatg catcaaactc gaagccaata cgtgtggaaa atgtgaaatt tgactggtca
     1021 cacggtcatg ttctgctgca tcaatctgaa caaaacgtgc ccccgttcca gataacaagt
     1081 ctgtcaatcg gggaaccgca tcctgcttgg tagcctgaca cggtgtcata atgtagtaag
     1141 cattctcaaa tagatggaga tctgctgcac tggcaccaga tttatgacta cctgccatag
     1201 ggtgtccacc gataaaatta atttgtcttg gcgccaaatg ttcctcagca gcagccacaa
     1261 ttgcagactt cgttgaacct gcatctgtaa tcaagaccgt atccttcaat tcaagggcac
     1321 taaattcttc aatcaaacgc aaagaaactt gaattggaac acaaagaaga atcacatcag
     1381 cctgctttgc tacaccctcc aattccgttg atacctcatc aaccatcccc ttgcccaagg
     1441 caatttggca tgattgttcg ttaggatcaa agccaatgag gtgatagtca ggatggtctt
     1501 tgcgaataca gagcgcaagt gagcttccaa tcaaccctag acctacaatt aaaatcgttt
     1561 ttttcattaa ttacctagaa atttttgaca tagtcgcgat gactggctac tgcagctttc
     1621 aattcctcca tattatcgga agagaacttc tctaggattt ctgtcgctag agttgtagct
     1681 acgactgatt ccatgactac accagctgct gggagagccg ttggatctga acgctccaca
     1741 gtagctttat aaggctcatg agtgtcaatg tcaaccgaca tcaatggctt atagagggtc
     1801 ggaattggtt tcataacacc acgaataatc agagcctcgc cattggtcat accaccttcg
     1861 aaaccaccta gattattggt tcggcgtgta tatccttttt cttgggacca gagaatttcg
     1921 tccataactt gactcccttt gagatagcca gcttggaagc ctactccaaa ttctactccc
     1981 ttaaaagcat tgattgaaac aacagcctgc gctaattttg catctaactt tttatcccat
     2041 tgcacatatg accccaagcc agctggaact cctccaacaa cagtctcaat cactccgcca
     2101 atagtatctc catctcgctt gatttgatca atataggctt tgatttcttc ttcacgctct
     2161 tggttgacaa tggatacttc tgactgggct gcacgctctt tgatttctgc tacgcttaaa
     2221 ccatctggga catcaatttc cttccctccg aaaacgacta catggttagc aatttcaata
     2281 tccaactcag ccaaaatccg tttggcaacc gcaccaacag ccacacgcat agttgtctca
     2341 cgcgcgctag aacgctccaa ggaattacgc aaatcatcaa agcgatattt cataccacca
     2401 actaggtcag catgtcctgg tcgtggatgt ttgatacgac gcttactttt gagtttctct
     2461 tcgatgtcct caactgccat aatatccaac catttttgat ggtctttgtt ggtcacattc
     2521 aatgtaatcg gggcaccggt tgtcttaccg tgacgaacac cagctgtaat ctctacgcga
     2581 tctgtttcaa tctgcatacg actaccacga ccgtatccac cttggcgccg ttttaactct
     2641 tcattaatat cctcggctgt taaaggaaga cccgctggaa ctccctcaat aatggctgtt
     2701 agacgtggtc catgagactc accagctgtt aaataacgca taacttaatt ctccaaaatt
     2761 tccagaaact tacgagtatt gcttaaagac atttgtcctg gtgcagatgt ttcatctaag
     2821 ctggcaaaag tccagcagga gcctgtaatc acacctgcaa tacgtgttaa cttccctagt
     2881 tcacccatgg cgattgtcgc aaatatctgc tcagtgttca gtgatttaaa accacgagta
     2941 taattcataa catctaggac atcctgctct gtcttagcca ttacagccat tttaacaaca
     3001 gctggtgata ggctagtcag ttcagacatg atttccatat aattagacgg tgtttcttca
     3061 aaattatgat aactcaacac aagattagga aactccaaca tctgctcaaa aaccgactta
     3121 taagagtagt attcaaaatc aatataatct ggctgataaa gcgttgctac ttctttgatt
     3181 acattgacat attcttgatc gtccaaatcc aagtgaccgc cttcacgtgt tgtccgaatg
     3241 gtgaaaacta cttccttacc actacatttt tcaaatatag caggtgctac tcttaaaata
     3301 tcatctttcg gaagatagtc tgctcgccat tcaacaatat ctgcctctgc tagacgttct
     3361 acatcaatgg tctctacctc ttccagattt ctaggcataa taggaactac tattttcatt
     3421 tatctacctg tatcgtaaag acctttaggt aattactact ttcatcattt ttattgatta
     3481 caaaatcggc tggcaagcga taagttgcct catagcgatg ttttctaaca tcaaaccctt
     3541 tttcaatctg ccgcttaaat ttttcaacag aaacatttgc ggcgttggtc gaagcgataa
     3601 tagttccacc tgtatttagt atttccaaag attgagaaat caacttgtga taatctttgg
     3661 ctactgaaaa ggtttgtttc ttatttcgtg caaagcttgg cggatccaag acaatcacat
     3721 cataagttaa gcctttacgt ttggcatatt tgaaatattc aaatacatcc attactacaa
     3781 agtggtgatt atctaagtct aaactattaa ccttaaagtg agctttagaa agttctcgcg
     3841 agcgtttggc taagtcaaca gaagtcgttt cctctgctcc gcccatggca gcagcaatag
     3901 aaaatgctgc cgtatacgag aacatgttca aaagtgactt gcctgccgcc aggccatcca
     3961 ccaaggaacc acgtacctca tgctgatcca aaaagattcc cgtcatcagt ccatcattca
     4021 taaagacctg atagtgaact ccattttcca acacagtgaa atactcaggg gcttcttctc
     4081 cataaagatg agcagactca aaggccaaac ctttaaaacg aatcttctca taagctcctc
     4141 ttacttctgg aaatacctgt tgaaatgcag caacaatcat ctctttgaca gaataaataa
     4201 aggtattata ccatgaaaaa actgcgtatt catcatacaa gtcaaccgtc attccaccaa
     4261 aactatctcc atcttgatta aataaacgaa aagcgttcgt catctcagat tgataaaaat
     4321 gttgacgttt ctgtttagca tccacgaata atttaacaaa aaaatccatg gtcaagtctt
     4381 gattgcttct actaaacagc cagcctatcc ccttattctg tttggacaaa taagcctgac
     4441 ccaaatagct tccagactct gaatacaaaa cagccagcct gtcttcatca aatgaaacac
     4501 ctgaaaaatc agcgctatcc aagagttgaa tccctctgtt tattttctgt tctgcaatac
     4561 ggtttacctt tattttcata gaattattat aacatgtttc ttcatatatg ccaaaaaaac
     4621 tctaaacttt gaggcttatt ttagaattat ggtataatga actaaacttt tagaaaggac
     4681 catgaggttt tattgtgaaa aaactaattg actatgtcaa gaagagcgga cagactagac
     4741 ttggtttcgt cttgattttg ctaatcattt actggctcaa aacactctgg gcctactatg
     4801 ttgatttcaa cttagataca caagggtttt tccaaaattt tattgctatt atgaatcctc
     4861 taccagtcgg gattttatta ctcggtctct cgctttatat aaagaataca aaagtcttct
     4921 atagcgtagc gatgatcatt tatattattc tttccgtctg gctcttttca aatgcagtct
     4981 attacagaga attttccgat tatatttcca tctctactat aatggctacc tctagtgtat
     5041 ctgctggatt aggagaagca gctcttaagt tatttaggct atgggatatt gtctattttc
     5101 ttgattttat catttttgga atcctagtct atcgtaaact actccggtgg gataaaaaga
     5161 catttcataa aaaatctagc tttgctatta cagcactatc agtcatgctt ttctccatca
     5221 acctcttttt agcagaaatt gatagaccag agctacttac gagaggattt tctaatactt
     5281 atatcgtcag agcccttggt ttacctgctt tccttggtta caatgctaat caaacctaca
     5341 atgcccacaa gaatcgttct ggagcatcag ctacagattt ggagcctgtg aaacaatata
     5401 ttacagaaca ttatgctcag ccgaatgaca actacttcgg tattgccaaa ggacgaaatg
     5461 ttatctatct ccacctagaa agcttacaac aatttgttat tgactataag ttgaacgtca
     5521 acggtgtcga atacgaagtc acacctttcc tcaattctct gtatcattct agctctactc
     5581 tcgccttttc taatttcttc aaccaagtga aggcaggtaa aacctctgat gccgaaacca
     5641 tgattgaaac ctcacttttt ggactcaatc aaggctcttt catggtccaa tatggcggaa
     5701 gcaataccca gcaagctgca ccatatattc tctctcaaaa tggtggctat acatctgctg
     5761 tattccacgg aaatgcagcc agtttctgga ataggaacaa cacctataaa caatggggtt
     5821 acaattactt ctttgatcaa tcctatttct ctaaagccac tgctgaaaat tcattccaat
     5881 acggtctgaa tgataaaatc atgttcgccg actctatcca atatctggaa catttacagc
     5941 aacccttcta taccaaattt atcacagttt ccaaccacta cccctacaca accagcttga
     6001 ttggggacga gattggcttt ccattagcca atacagatga tgaaaccatt aatggctatt
     6061 ttgctactgc aaactatctc gatgcgtcca taaaggcatt tttcgattac ttaaaagcga
     6121 ctggcttgta cgaaaactcc gttatcgtcc tctatggtga tcactatgga atttctaatt
     6181 cacgcaatcc tgacttggca ccgttgattg gaaaagattc tcagacttgg tcagattttg
     6241 acaatgccat gatgcaacgt gttccatata tgattgttgt acctggaatg accaacggcg
     6301 ggatttttga tacctacggt ggtgagattg atgcacttcc tactctcgaa catcttttag
     6361 gaatcaaatc cgataactat cttcaagttg gtcaagacct actttccagc caacacagcc
     6421 aaattgtggc tcttcgaacc gctggcagtt tcatcacacc aaaatacact agctacgaag
     6481 gtaaaatcta ctatacagaa actggtcttg aaattacaaa tccagatgag acaactcaga
     6541 aagaaattga tgcaattaaa gaagctaccg cagcacaact agcgactagc gatgccattc
     6601 aaactggtga tcttatccga ttcttcgaca atggattgcc agccttggat ccaagccaat
     6661 ataactacat tgactcattg gctgccgaga ttgcactaga gaataaactt ggcaaacaat
     6721 caacaagttt gtatagcaca aacggcaata agtcgacaat tgaactattt aaagcaccaa
     6781 gctacctaga acttcacggt agtacaactg ctactagcac accaaatacc ggcactagct
     6841 caacaagtac cagttcaaat tcacggtaat acactaaaag gcttagaatc atgtcagtta
     6901 ctacattgat tctaagcctt tttatactca atgaaaatca aaaacagact agctccaaag
     6961 gtttggggaa cctttggagg ttggagatag agcgaacgta gttcgtttct acttagcaga
     7021 tagaaccctg ttactatttt gtttcaaggt aacaggctga aaggctccac tggagccttt
     7081 cactcatcaa atcaagtcaa caacgtctga ttttgatttt cgaagagtat tagaccacta
     7141 tttcaaatca ctcatatcta tttcatcaag gaaatataaa atcctcacta ggctcttcct
     7201 ctgttgatga ttttttaggg atatctatgg gagcagcagt actactttca gttgcttttg
     7261 tatcttctgt tgtagtttca gcttctgtta cagtttctat atctgtcgct tctggactag
     7321 gtgtcaacaa atcatctaac cagctatctg cgtcctcttc tccattaggg acatctggag
     7381 tattcggcaa tggattccca ggcaaccaag tatcacctgg ctcaactgtc ccatctttgc
     7441 tatcactttc aactttatcg aaatgaatat cgtagacagt atcttccaaa ataggtgtat
     7501 cccatgctac atttggaata acaggttttg tataatctcg tttataacct gtatcactct
     7561 tcaaagatgg cggaaaaatt ccagcttggt tcagtgttaa tccttttcta acaaggtaaa
     7621 caactgtttt actagttccc aaatgtgtct gaagttgacc gccgtcacct gctcgaaatg
     7681 caagagctac ataattcttt ggatcatgcc aatagtaatt tggatgtcta cttggatcat
     7741 tgatgtccgt tggattatcc atattttcgg caatatatgg ccctatacga ggagctgttg
     7801 tcccaaatgc tgccaagctc gttgtggcac taacataatc tgtatctttt tctccaccgg
     7861 taataactgt cctaaactcg atgaattcat aatctttgct tggaaccggt tctggtacaa
     7921 caagcccctg ctccttagct tgtttccaag ttaagtcaga tttcacaaag tatatgaacc
     7981 ctgctcgaat ctcaccattt aattttaaac gaccattggc tatcgcacta tatttaacag
     8041 gtacatagtc tgataaatca atatttgaaa catcatcact tggagtgtaa atattttttg
     8101 tcggcttacg ataaacatta tcaaaatcag cataaaaact ctgatgttct cttgccatct
     8161 tgccagactt tggcaaagta gcattccaac cattaaagac gcgtcccttt tgttgaaggg
     8221 tctgcggata ggcgatgttc aaatcctccc aatttaaatc tttacgaacc aaataataaa
     8281 ctctcttcgt ttttttacca ttaacgacaa aatcaccaaa gtcactattt gtactgaatg
     8341 ttactagaat gaaattttta atatcctgaa gaccttccgg atgatacttt tgatggacac
     8401 cattacgtgt taaattttct gttgaacctt ctgtataaaa aggtccatat actaattgtt
     8461 tctcaatagc aagatttacc gtttcggtct taacatcacg ctcaggatca tctggcaaac
     8521 taggatcaat tttataaacg tgcgcatggt ctccatggcg tacactcata tagttcccat
     8581 caattgtaat gtcctcacgt tttactttaa aacgctccat gtactgttta atgagacgtt
     8641 cttttctctg ttcaagagtt tctccatcct tttgtttaag aatacgatct tccggacttt
     8701 caaacggctt cgttgtatca atttccttta ataaaacaat atgtgtgtga tctccatgcg
     8761 gatactctaa tccaatttga ccgtcaacga tgactttctt aaaacgtgat ggctctaaat
     8821 tcagttcttt tgctaagtag tccaatttgg tttgatactc agtattttct acctcaggtt
     8881 gtgccttctt atccgattca acatttggtt tatactgatt cacaagataa gaccatttac
     8941 tccttttcaa tgtttcaaaa tgaataagat gcaaatgttt cccatgcctt actagaatcc
     9001 cggtttccgt tacaccgctg atattttgcc catcaaacaa gaaaccgtca ctcgtttgaa
     9061 aatcaatccc cggaatccct tcctgttggt gaacgatggg aagagttggc gattgacgac
     9121 tatcagaggt caattgtctg tgtgtcgtgc cacctaagct tgattttaaa atatagtgat
     9181 aatgatcccc atgccgaaca gtatagccat tggcatcctc tgcgacaata tctttaggat
     9241 caaaaacgta ttcatcttcg atctgtttcg agcctgacga taattgactt ctcttaggtt
     9301 gagaagaatg atttttttct tgatagtttt caggaattaa ataagcccat tttgtattct
     9361 ttaaatctga ataaaagaaa aagtgcttat gatcgccatg attgacaata attcctaaat
     9421 cagttttttc ctcaatttgt gactcatcag tgagtaaaaa accatcatcc gtcgccttat
     9481 caatccctgg aacttccttt ttcgaatctg aaggaatctc ttttgattgc tttgattgcg
     9541 atgtttctat gcttgttgtt ttttgatggt tcgattgctc tctatagtta gacgcttgat
     9601 aagtaaccaa tgctacagca caaataagcg atactcctgc tacataaagc aaagtcttac
     9661 cttgtttcat atctatgttt ctctccttat ttaccattat tatattaccg acacgataaa
     9721 tgtaatcaac tattgtaaaa tagcatcacc acatccactt attatagtct tttagtttac
     9781 ttttagtacg aggcaatgag ccgcaggcat aactgaagtt aggcaaggcg agttgatgaa
     9841 gtaacaaaag aaaaactaaa tgacgataaa gttaaaaccg tcgatttatt tactagttaa
     9901 gtatatcact catatcgaaa tttagcaagt agtgagctac tttccaaaaa taaagaacct
     9961 ggttcatcac taccaagttc tttacatttt tatacttatt tagcaagttt tgctttagca
    10021 gcatctgcaa gagctgtgaa agctgctgca tcgttcactg ccaagtcagc aagcattttg
    10081 cggtttactt cgatttcggc caatttcaaa ccgtgcatca attgtgagta tgacaaaccg
    10141 ttcatacgag cagccgcatt gatacgagtg atccacaatt tacggaaatc acgttttttc
    10201 tgacggcggt cacggtatgc atagtagtaa gagttcatga cttgttcttt cgcagtgcgg
    10261 aacaagagat gtttagctcc gtagtaacct ttagctaatt ttaaaatacg tttacggcgt
    10321 ttacgagaaa caacgccacc tttaacacgt gccatttaat tttcctccaa atgataatct
    10381 aaattctagt aagtcggctt atttcaaacc tgtaagcatt gatttgatac gcttgaagtc
    10441 accagcatgt accatacctg ctttacgaag gtgacgacgt tgttttttag ttttaccgtg
    10501 gaaacggtgt gaagtataag cgcggaagcg tttcaatcca ccagaacctg tacgtttgaa
    10561 acgtttagct gatgcgcggt gagttttttg ttttggcatg ttaattctcc ttttgcttaa
    10621 aaagcttatt ttttatctga agctggggcc aattgcataa acatttgacg tccgtccatc
    10681 ttagcgcgtt gttcgataat ggcaatatct tgggttgctt cagcgaactc agctaatact
    10741 ttcgctccaa cttctttgtg ggtaatcata cgacccttaa aacggatcga aaccttaact
    10801 ttatttcctt tttcaagaaa tttacgggca tgacgaagtt ttgtctcgaa atccccttta
    10861 tcaataactg gacttagacg cacttctttt acagtgacaa cactttgttt tttgcgttgt
    10921 tctttctgtt tcttctgata ttcaaatttg aacttaccgt agtccataat tttcgctact
    10981 ggtggtttag cttggggttg aatcaatact aaatccacgt tggcgctatc agcaatggcc
    11041 tgagcctcat tgagtggttt gattcccaac tgttcaccct caagaccgat aagacgaact
    11101 tcacgcactc ggatttcatc attgatgaac aaatcttgct ttgctatggc ttttcacctc
    11161 attttatatt tttcgagaaa aacgaaacgg acttgctaaa caagtccgca ctacaccatt
    11221 attctttccg aagaaatctt aatccgtaga gccagacaac gttagtcgca aggcgagaag
    11281 ttctcacttc tgcttttctc aatacgacta ttctaacatg tctaaagcta cctgtcaaga
    11341 atttctttcg ctttttcgga aataaatttc actacacctt cgatagatac tcctgttgta
    11401 tcaaaggtaa tagcatcatc tgctgccttt aaaggagaaa cttctcgatt ggaatcttta
    11461 tagtcacgag ccgcaatctc ctctttcaag gattccaagt ctgtctcaat gccacgttcc
    11521 gtattttcct taaatcgacg aagggcacgc tcttcaacag aggcaacaag gaatattttg
    11581 agttctgctt gcggcaatac aaccgtacca atatcacgac catccataac aatcccacca
    11641 gcttgcgcaa tgtcctgttg caggcggacc aattcttccc gtactagagg aagcgctgct
    11701 accgctgata cattatttgt tacttgatta tctcgaatag gcaaagtaac atcctcatca
    11761 ccgacaaaaa caagttgctg accgttttca tcacgcccaa atcgaatggg atactgggca
    11821 agcaagtcca cgattctatt ttgatctgtg acctctatgc cgtttgtcaa ggctagatag
    11881 gtagccgagc gatacatagc tcctgtatct aagtaagtat atcccaaatt tttagcaata
    11941 attttagcaa ccgttgactt tccacttgaa gctggaccgt caattgcaat ttgaattgat
    12001 ttcataactc ctctttatct aatacgaact acatccccag gatgcgcaat ccaagaccct
    12061 gtagtcattt tctctggatt caaacgctct agctctgcaa ttgaaatccc accacgagca
    12121 gccagttgtc ctacaccttc acctgcttga acagtaaggg tagagccatc cgttgcttcc
    12181 gctgggctag aacttgtttc tgtttctgtc gtgtcagtcg aagtagattc ttgtgtctca
    12241 gcagccgatg aagagctctc tgctacgact gttgtagtgc ttggggtata aaattctttt
    12301 gttgaatctg ttttactacc gccaccgatt gagagataga cagccataat tccaataatc
    12361 agtacgataa caaagaaaat aattgccaaa actgtaaata ctgttgtatc cgctaccccc
    12421 ttactcagac gttctttacg actagttgtt tctgtgtggt aaacttcttc attccatggc
    12481 tcttgtgaca ttattgagaa aactccttgt atttttatta aaatatgatt acaatactaa
    12541 tatgaaagta aaacttattc ctgaacggtg cattgcttgc gggctctgcc aaacctactc
    12601 atctctattc gattatgatg acgacggtat cgttgttttt tcagacgaat cacaatttga
    12661 gaaaacgatt gaacccaatc cagatattct taaagccatc aaatcatgtc ctacaaaagc
    12721 catttcagcg gattgatttg gctttttttg tataatactc ttcaaaataa tcctgtttta
    12781 ttaaatggtc tattaattca attttaccct tgaagcaact atgaatagct aaatggttaa
    12841 taaagtattt ttttgtccat ttacgcatgt gatacacctg cccattggga aattcttgtg
    12901 aaaatatgcg aatggctaga taagaaactt caattttttc tatggtagca atttcaattt
    12961 tctgtactgc aaaaggatta gccgaagcaa tccgtaaatg ctcatctttt atctcgaaat
    13021 agcgatgtaa gccgagcact actaatacag caaagagtga gacaagtaga ataaataatc
    13081 ttgaaacttg caacttctcc aataaaagtg tcataccaat aaaaataggt gtgaaggtaa
    13141 gtgaccagta tatgatggcc catgataaat ccggttgcca atggtaacga attttaccaa
    13201 aaattttgat cata
//
LOCUS       DAZQCJ010000052        12473 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000052 DAZQCJ010000000
VERSION     DAZQCJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12473
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.053"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(26..754)
                     /locus_tag="ACHU66_001322"
     CDS             complement(26..754)
                     /locus_tag="ACHU66_001322"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFR3389603.1"
                     /translation="MRLDKFLVDCGVGSRTEVKKLLKNKQVTVNGQVETSPKQQIDEK
                     SDQIAVAGQVLSHETFVYYVLNKPKGVISATEDDRHRTVLDLLDNTARQKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVMLSPKKHVDKLYRAQVDGIMTQEDVERFAAGIELKDLTC
                     QPAQLTILSTDEAKQTSLVDITIREGKFHQVKRMVQACGKTVTDLQRLTMGPLRLDPK
                     LALGEYRRLTAAELKQLEVFGVEL"
     gene            complement(745..1371)
                     /locus_tag="ACHU66_001323"
     CDS             complement(745..1371)
                     /locus_tag="ACHU66_001323"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR3389604.1"
                     /translation="MLENNRLNELILRFPEDVQLIFKDMIPSYQLGYVDYVYQTDSYA
                     TQNRRIKNLAKNFRRMDYFYIMPLPQDLALEIANQWRYQPPLDAYTISANPETYAEMI
                     SPEARGDRFFAVIRNAALMGYFCIDQDGETVDIRLGMKPSLTGQGNGRAFYQAIEDYL
                     VEHVQPASIKLTVASSHQVAQKLFHALGFTEIEKQSEYIKMEKKLVCD"
     gene            complement(1408..2571)
                     /locus_tag="ACHU66_001324"
     CDS             complement(1408..2571)
                     /locus_tag="ACHU66_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR3389605.1"
                     /translation="MKRILEKASLLALSTMLVSTFAVSPAIPQMIEHFAQQGIAAAQV
                     ENLITVTSFAIMVTLLANGLVVRFLSERNIIIAGLLLMAIGGALPMFLTAYPLILLAR
                     ILLGLGIGLINARAINIIGNFFTGQERVQMMGLRGSAEVLGSAGLTLLVGWLTQFGWQ
                     PAFLVYLFALVILALFLLFVPKEELLARMEVKVEENAPKVKLDKTMWMMGIYLAFLAF
                     FVINVNTFLTIRIPQIVLAKGIGTAQQASLILSLMQVMGIVAGTVFSSLVGRLKGWLL
                     AVSYVVFGLAVVGIAFADNLWVLGLGGMVSGFFYSIILTIVFSQVTDRAPKALLNAVM
                     TIVLMGCNIGGATSAILPTYLEKLNPTPTGAFGIYAIGCALISAGLIYKQLKK"
     gene            2701..3315
                     /gene="def"
                     /locus_tag="ACHU66_001325"
     CDS             2701..3315
                     /gene="def"
                     /locus_tag="ACHU66_001325"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR3389606.1"
                     /translation="MSVIERLTKAAHLIDMDDIIREGHPTLRQVAEEVAFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMKLRGGVGLAAPQIDVSKRIIAVLVPNPEDEEGNPPAQ
                     AYSLQEVMYNPKIVAHSVQEAAMEGGEGCLSVDREVQGYVVRHARVTVDYMDKNGEKH
                     RIKLKGFNAIVVQHEIDHLNGIMFYDRIDPEHPFAVKEGMLVIE"
     gene            complement(3504..4289)
                     /locus_tag="ACHU66_001326"
     CDS             complement(3504..4289)
                     /locus_tag="ACHU66_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HFR3389607.1"
                     /translation="MTKQIHWDGALSQEGFDIIKGEGGVIVCPTKVGYIIMTADKAGL
                     ERKFDAKERKRNKPGVVLCGSMEELRELAQLTPEIDAFYQKHWDEDILLGCILPWKPE
                     AYAKLQAYGDGREELMTDVRGTSCFVIKFGVAGEQIAKEMWEKEGRMVYASSANPSGK
                     GNRGKVEGIGERIASKVDLIIEADDYVASIQPDKTIETRYEQGVMVSMVDKDGKLIPE
                     QGMDSRSISPCPVVIRKGLDIDKIMMHLSDHFNSWDYRQGEYY"
     gene            complement(4352..4945)
                     /gene="leuD"
                     /locus_tag="ACHU66_001327"
     CDS             complement(4352..4945)
                     /gene="leuD"
                     /locus_tag="ACHU66_001327"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018167039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR3389608.1"
                     /translation="MHQFTTWTGTTVPLMNDNIDTDQLLPKQFLKLIDKKGFGKYLLY
                     AWRYLDDNYTDNPDFILNQPDYQGASILISGDNFGAGSSREHAAWALADYGFKVIIAG
                     SFGDIHYNNDLNNGILPIIQPKEVRDQLAQLAPDQEITVDLADQLIRTPFGEFPFDIE
                     QDWKHKLLNGLDDIGITLQYQDLITEYEGNRPSYWQE"
     gene            complement(4945..6336)
                     /gene="leuC"
                     /locus_tag="ACHU66_001328"
     CDS             complement(4945..6336)
                     /gene="leuC"
                     /locus_tag="ACHU66_001328"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR3389609.1"
                     /translation="MSGKSIFDKLWDRHVITGQEGQPQLLYVDQHYIHEVTSPQAFDG
                     LREAGRQVRRPELTFATFDHNVPTVDIHNIRDVISKAQMDALAKNIEDFGIPHAAHGS
                     EHQGIVHMVGPETGRTQPGKFIVCGDSHTATHGAFGAIAFGIGTSEVEHVLATQTIWQ
                     VKPKRLLVEFVGQAQRGVYAKDFILALIARYGVDCGLGHVVEFAGPVIDRLSMEERMT
                     ICNMSIEFGSKMGIMAPDQTTFDYLRGRECAPADFDAAVADWKELYSDADTVYDKHLV
                     LDVSDLAPMVTWGTTPAMGVSFDEIFPAIRDHNDERAYAYMDLAPGQKAADIPVGYVF
                     LGSCTNARLSDLQLAARIVKGRKIAEQVTAIVVPGSRPVKRAAEKLGLDKIFMEAGFE
                     WRDPGCSMCLGMNPDKVPAGVHCASTSNRNFEDRQGFGAKTHLCSPAMAAAAALYGRF
                     VDTRQLDILKEGV"
     gene            complement(6333..7385)
                     /gene="leuB"
                     /locus_tag="ACHU66_001329"
     CDS             complement(6333..7385)
                     /gene="leuB"
                     /locus_tag="ACHU66_001329"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR3389610.1"
                     /translation="MTKKIVALAGDGIGPEIMEAGLAVLEAVAGQVGFDYEIEERAFG
                     GAGIDAAGHPLPNATLQACRQADAILLAAIGSPQYDDAAVRPEQGLLQLRKELGLFAN
                     IRPVKIFDSLKDYSPLKADRLDGVDLVMVRELIGGIYFGEHILETDQASDSNTYQAEE
                     IERVVRSAFDLAQKRQKKVTSIDKQNVLATSKLWRKVADEVAKDYPDVTLEHQLVDSA
                     AMLLITNPSRFDVLVTENLFGDILSDEASVLTGTLGVLPSASHAVAGPSLYEPIHGSA
                     PDIAGQGIANPVSMILSVAMMLEESFDLVQAGQTIRQAVEAVFAEGIFTKDLGGTAST
                     KEMTEAIIAQIEREEI"
     gene            complement(7394..8956)
                     /locus_tag="ACHU66_001330"
     CDS             complement(7394..8956)
                     /locus_tag="ACHU66_001330"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR3389611.1"
                     /translation="MRVIEFLDTTLRDGEQTPGVNFSIKEKVTIAKQLEKWGISAIEA
                     GFPAASPDSFEAVRQIAAAMTKTAVTGLARSVKSDIDACYEALKDAKYPQIHVFIATS
                     PIHREFKLQKTKEEILAQITEHVSYARERFEVVEFSPEDATRTELDYLLEVVQTAVDA
                     GATYINIPDTVGFTTPQHYGEIFRYLTTNVKSDREIIFSPHCHNDLGMAVANTLSAIK
                     NGAGRVEGTINGIGERAGNAALEEVAVALEIRKDFYDVTSPIVLKETLNTSELVSRFS
                     GIAIPRNKAVVGGNAFSHESGIHQDGVLKNPLTYEIITPELVGVKKNSLPLGKLSGRH
                     AFVEKLKELDLYFEEGDVASLFACFKKLADKKEKITDADIRALVAGTEISNRDGFQFK
                     DLRLDSQSDGSIQAQVTFINQDEEEVVVVESGKGSVEAVFNAIDQFFHQEISLERYHI
                     DAITDGIDAQARVLVAVENKATETIFNASGIDFDVVKASAIAYIHANVMVQKENSGQI
                     DKKLSEKDLPHI"
     gene            complement(9415..10668)
                     /locus_tag="ACHU66_001331"
     CDS             complement(9415..10668)
                     /locus_tag="ACHU66_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFR3389612.1"
                     /translation="MNVQSVQKFAAKISNQRHLIALRDGMATAMPLIIVGSVFMLIAD
                     FPIPAFSAWLSDIGISEFLWKATDATFGIAGLVVTFAVAYHLAKRYDVDRLSAGVLSI
                     ASFVLLTPVLSSDAGNGFPLKYLGTAGLFVGIIVALISTEIFSFFVKRNIVLKMPDTV
                     PPNVAKAFSAIIPGLFIISLWLMILILLKATGIDNIHALIADTVAKPLGLVTQTLPGI
                     LVVIFIQCFFWMFGVHGAQVTGPILEPLLLQNSEANRLAYQAGEELPHIIIYEFLYNF
                     VFSGGAGCVMALAILTFFLSRSQENKALGKLSIAPVSFQVAEPILFGFPTILNIKMAI
                     PFVLAPVVTTIVTYFAMDWGLVAKPIGAVVSWTTPPVIAGFLASGGRISGAVIQIVTI
                     LINMAIYYPFFRMDDKAKLEREQGK"
     gene            complement(10700..11623)
                     /locus_tag="ACHU66_001332"
     CDS             complement(10700..11623)
                     /locus_tag="ACHU66_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFR3389613.1"
                     /translation="MNKRLLDYSASDFLSASPMELKQAILASEGRTVMAENVPSRSSF
                     LSGVSNAEMERAAGADLIMFNALDLFHPLIAEIPEDVQENPIVWIKKALGRAIGVNLE
                     PVDLEANMVETRAEISIGRIVSPKTLKKATELGFDFICLTGNPGTGVSNQSIFSAISM
                     AKEYFSGLIIAGKMHGAGVDEPVMNLEIAKQFIHSGIDILLVPAPYTVPNFVAEELKE
                     IVDYVRSYNKDQTIENKVLVLTANGTSQDSSDTDTIKKIALTSKACGADIQHIGDSFN
                     GIALPENIFALGQAIRGKRHQLTMLARSIIR"
     gene            11780..>12473
                     /locus_tag="ACHU66_001333"
     CDS             11780..>12473
                     /locus_tag="ACHU66_001333"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR3389614.1"
                     /translation="MRDNSTKSPLYYQLFDSLVEYIQKDLQPHDQLPTEKEIGTDYSV
                     SRTTVRLAMQELEKHGYIYRIQGKGSFVSSLKQENINSFFSLELKNHYDGLETADFES
                     HLVSFTKENGSLALKHLFGLSKQTNLVRIQLEHLLHSELVATETIVAKASYMPELTQE
                     SLEFEGLDSLFQKTGAITKAVDEHYSLARFMPTSSEIDALKITKSIFNQDNELMLTST
                     RIINIQKLPYRNF"
BASE COUNT         3604 a         3037 c         2484 g         3348 t
ORIGIN      
        1 tcgggagcaa gtctcttcta tttatctata attccacacc aaagacttct aattgcttta
       61 attcggcagc agtcaacctg cggtattctc caagcgctaa ttttggatct aagcgaagag
      121 gccccatggt caatcgttgc aagtctgtca ccgtctttcc acaggcctgc accatgcgtt
      181 tgacctggtg aaatttccct tctcggatgg tgatgtcaac gagcgaggtc tgcttagctt
      241 catctgtaga gaggatggtc aactgggctg gttgacaggt taagtctttc agctcaattc
      301 ctgccgcaaa tcgttcgaca tcctcttggg tcatgatccc gtccacctgt gcacggtaga
      361 gtttatcaac atgttttttc ggagaaagca tgacatgagc cagctgaccg ttgttggtta
      421 agaggagcaa gccatgggta tcaatgtcca agcgtccaac tggaaaaact tccttctggc
      481 gagctgtatt atctagcagg tccaagacag tccgatgacg gtcatcctcg gttgctgaaa
      541 taactccctt gggtttgttc aatacataat aaacaaaggt ttcatgactc aagacctgac
      601 ctgctactgc aatctgatct gacttttcat caatctgctg ttttggggag gtttctactt
      661 ggccgttgac tgtgacttgc ttgtttttca ggagtttctt gacttctgtc cgactgccaa
      721 caccgcaatc cactaaaaat ttatctaatc gcatacgagt ttcttttcca ttttgatata
      781 ttctgattgc ttttctattt ctgtaaaacc aagagcatga aagagttttt gagcaacttg
      841 atgagagcta gcaactgtca gtttgatact agctggctga acatgctcta ctaaatagtc
      901 ttcaatagcc tgatagaaag ctctgccatt tccctgacct gtcaaagaag gtttcatacc
      961 caaacgaatg tcaacggtct cgccatcttg gtcaatacag aaatagccca tgagggcggc
     1021 attgcggatg actgcaaaaa agcggtcccc acgcgcctct ggtgagatca tctcagcata
     1081 agtctcgggg tttgcactaa tcgtataggc atccaaaggt ggttgatagc gccattggtt
     1141 agcaatttcc aatgccagat cctggggcag gggcatgatg taaaagtaat ccattcttcg
     1201 gaaatttttt gccaaatttt taatccgacg gttttgggtg gcgtaactat ctgtctgata
     1261 aacataatcc acatagccca actgatagga gggaatcatg tccttaaaaa tcaactgaac
     1321 atcctcggga aaacgtagta ttaattcatt taagcggtta ttttctaaca taagaatatt
     1381 ataccaaaaa ggagcggaaa tccgctccta cttcttcagt tgtttataaa tcaagcctgc
     1441 gcttatcaag gcacagccga tggcgtaaat accaaaggca cctgttgggg ttgggttgag
     1501 tttttccaga taagtcggta agatagcaga tgtcgcaccg ccgatattgc agcccataag
     1561 gacgattgtc atgaccgcat tgagcaaggc cttgggtgca cggtctgtta cctgactaaa
     1621 gacaatggtt agaatgatgc tgtagaaaaa tcctgacacc atgccaccga gtcccagcac
     1681 ccagagatta tccgcaaagg caatgccgac aaccgcgagt ccaaagacca cgtaggaaac
     1741 agctaaaagc caacccttca aacgaccaac caggctacta aagaccgtcc ctgccacaat
     1801 ccccatgacc tgcataagac tgagaatgag actggcttgc tgagcagtac cgatgccctt
     1861 ggctaaaaca atctgaggaa tacgaatggt cagaaaggta ttgacgttga taacaaagaa
     1921 ggctaagaat gccagataaa ttcccatcat ccacatggtc ttgtccagtt tgaccttggg
     1981 cgcgttctcc tcaaccttta cctccatgcg agccagcaat tcctccttgg gaacaaagag
     2041 aaggaagaga gccaagatga ccaaggcaaa aaggtagacc aagaaggcag gttgccaacc
     2101 aaactgggtc aaccaaccga caaggagggt gagccctgca cttcccaaaa cctctgctga
     2161 accacgcaag cccatcatct gtacccgctc ctgaccggtg aaaaaattac cgatgatgtt
     2221 aatggcacgc gcattgatta agccaatccc caaaccaagc aagatgcgag ccaataaaat
     2281 cagaggataa gcagttagaa acatgggcaa agctccaccg atggccataa gcaaaagccc
     2341 tgcaatgata atgtttcgtt cagacaagaa acggacaact agaccatttg ccaatagagt
     2401 aaccataata gcaaaggagg tcaccgtaat taagttttcc acctgggccg ctgcaattcc
     2461 ctgctgggca aaatgctcaa tcatctgtgg aatagctggc gagacagcaa atgtcgaaac
     2521 cagcatggtg gatagggcca acaaactggc tttttctaaa attcttttca tttttctccc
     2581 tttcaacgtt cgtcttttac tagtataaca gatttgaaag cgaatggata gaaaagtccg
     2641 acttttattc tctcccattt catgctatac ttatcttaac tatattcaaa ggagagaatc
     2701 atgtctgtta ttgaacgatt gacaaaagct gcccatttga ttgacatgga tgacatcatc
     2761 cgtgaagggc atccaaccct acgccaagtt gccgaagaag ttgcatttcc actatctgat
     2821 caggaaatta ttttaggcga aaaaatgatg cagttcctca aacattcgca ggatcctgtc
     2881 atggctgaaa aaatgaaact ccgtggcgga gttggtctag ctgcacctca gattgacgtt
     2941 tccaaacgca tcattgctgt cttagtccca aatccagaag atgaggaagg caatccacct
     3001 gcccaagcct actccctcca agaggtcatg tacaacccta aaatcgtggc acattctgtc
     3061 caagaagctg ctatggaagg gggcgagggc tgcctatccg ttgaccgcga agtccaaggt
     3121 tacgttgtcc gccatgcgcg cgtgactgtt gattacatgg acaaaaacgg agaaaagcac
     3181 cgtatcaaac tcaaaggctt taatgccatc gtggtccaac acgaaatcga ccacctcaac
     3241 ggtatcatgt tctacgaccg cattgaccca gaacacccat ttgccgttaa agaaggcatg
     3301 ttggtaattg aataaacata agaaactgac tgaaaaattt tcagccagtt ttttatgtga
     3361 gtacgaaaaa acgacccttt cggatcgatc tcaataatag tcttttagtt tacttttagt
     3421 actaggcaac gagccgcagg cataactgga gttaggcaag gtgagttaac gacgtaataa
     3481 aagataaact aaatgacgat atcttagtaa tattcccctt gtctgtagtc ccaagagttg
     3541 aaatggtcag acaagtgcat catgatttta tcaatgtcca gacccttacg gataacaact
     3601 gggcatgggc tgatagaacg gctatccatg ccttgttctg gaatgagttt accatctttg
     3661 tctaccatag acaccataac cccttgctca tagcgagttt caatggtctt gtctggctgg
     3721 atagatgcca catagtcatc cgcctcgata atcaaatcca ccttagaagc gatacgctca
     3781 ccgatacctt ctaccttacc acgatttccc ttacctgacg ggttagcaga tgacgcatag
     3841 accatacgac cttctttttc ccacatttcc ttggcaatct gctcgccagc tacaccaaac
     3901 ttgataacaa agcaagaagt cccacgaacg tcggtcatca gctcttcacg accatcgcca
     3961 taggcttgca acttagcata agcctctggt ttccaaggta ggatacaccc caacaaaatg
     4021 tcctcatccc aatgtttttg gtagaaggca tcaatctcag gagtcaactg agccaactca
     4081 cgaagctcct ccatgctacc acaaaggact acgcccggct tattacgctt acgctcttta
     4141 gcgtcaaact tacgctccaa accagccttg tcggcagtca tgataatata accaacctta
     4201 gtcgggcaga caatcacgcc tccctccccc ttgataatgt caaatccttc ttgtgaaagt
     4261 gcgccatccc agtgaatttg tttcgtcata atagtttcct cttttctttt gttccatagc
     4321 agagcgatct gcttgttttc taatctttat tttattcttg ccagtagctt gggcgattcc
     4381 cctcatactc agtaatcaaa tcctgatact gaagggtaat cccaatatca tctaagccat
     4441 tgagcaactt gtgtttccag tcttgttcga tgtcaaatgg gaattcccca aaaggcgtcc
     4501 gtatcaactg atctgccaag tcaaccgtga tttcttggtc aggagccagc tgagctagtt
     4561 ggtccctgac ttctttgggc tggataatgg gcagaatgcc attgttcaag tcattgttgt
     4621 aatgaatatc tccaaaggag cctgcaatga taaccttgaa gccataatct gccagagccc
     4681 aagcagcgtg ttccctagaa gaacctgctc cgaagttatc cccagatatg aggatactag
     4741 cgccttgata gtcaggctgg ttgagaatga aatcaggatt gtccgtgtag ttatcgtcca
     4801 agtaccgcca agcatatagc aaatacttgc caaagccctt tttgtcaatc agcttgagaa
     4861 actgcttggg caagagctgg tcggtatcaa tattatcatt catgagcgga acagtcgtcc
     4921 ctgtccatgt ggtaaattgg tgcattagac tccctccttg agaatgtcta gctgtctggt
     4981 gtcgacaaaa cgtccataaa gcgcagctgc ggcagccatc gcaggactac acaagtgggt
     5041 cttggcccca aagccctgtc ggtcttcaaa gtttcgatta cttgtagagg cgcagtggac
     5101 acccgcaggg accttgtctg gattcatgcc cagacacatg gagcaaccag gatcccgcca
     5161 ctcaaaacca gcctccataa aaatcttgtc tagaccaagc ttttctgcag ctcgcttgac
     5221 agggcgactg cctggtacga caatagccgt aacctgctct gctattttcc gccccttaac
     5281 aatcctagct gccagctgca aatcgctgag gcgagcattg gtacaggagc ctaggaagac
     5341 atagccaacg ggaatgtccg cagccttttg cccaggagct aagtccatgt aagcataggc
     5401 ccgctcgtcg ttgtggtcac ggatggctgg gaagatttcg tcaaagctaa cccccatagc
     5461 aggcgtcgtt ccccaagtga ccatgggtgc caagtctgac acatcaagga ccaggtgctt
     5521 gtcatagacg gtgtccgcat ccgagtagag ctccttccag tctgccaccg ccgcatcgaa
     5581 gtccgctggt gcacactccc gacctcgcag gtagtcaaag gtggtctggt ctggagccat
     5641 aatgcccatc ttagagccga actcaatgga catattgcag atagtcatcc gctcttccat
     5701 gctgagtcgg tcgatgacgg gtcctgcgaa ctcgaccaca tgacccaaac cacaatccac
     5761 gccgtagcgg gcaatcagag ccaggataaa gtccttggca tagacacctc tttgcgcctg
     5821 accgacaaac tctactaaga gtcgcttagg tttgacctgc caaatggtct gggtagcgag
     5881 aacgtgctcc acttcagaag taccaatccc aaaggcaata gctccaaatg ctccgtgagt
     5941 cgctgtatgg ctatctccgc agacaatgaa cttacctggc tgggtcctgc ctgtttctgg
     6001 tccaaccatg tggacaatcc cctgatgttc cgaaccgtgg gctgcgtggg gaatgccgaa
     6061 atcttctata ttcttagcca aggcatccat ctgagccttg gaaatgacat cacggatatt
     6121 gtgaatatca acggttggta cgttgtggtc aaaagtcgca aaggtcaatt ctggccgacg
     6181 gacctgacgt cctgcctctc gcagaccatc aaaagcctgg ggactggtca cctcatgaat
     6241 atagtgctgg tccacataga ggagctgggg ctgaccttcc tgccctgtaa tcacatggcg
     6301 gtcccagagc ttatcaaaaa tagatttgcc actcatatct cttccctctc aatctgggca
     6361 ataatggcct ccgtcatctc cttggtagaa gcggtcccac cgagatcctt ggtgaaaatg
     6421 ccctctgcaa agaccgcttc aacagcttga cgaatggtct gacctgcttg gaccaaatca
     6481 aaactttctt ccagcatcat ggcaacagat agaatcatac tgacaggatt ggcaatgccc
     6541 tgaccggcaa tatccggtgc cgaaccatgg ataggttcgt agagactggg acctgctacc
     6601 gcatgactag cagaaggcag aacccctagt gttcctgtta atacgctggc ctcatccgac
     6661 aaaatatctc cgaaaagatt ttccgtcacc aagacgtcaa atcgactagg attggtaatc
     6721 aagagcatgg ctgcgctatc caccaactgg tgctccaagg tcacatctgg gtagtccttc
     6781 gccacttcat ccgccacctt ccgccataat tttgacgtcg ccagtacatt ctgcttatca
     6841 atgctggtga cttttttttg tcttttttgg gctaggtcaa aggcagagcg gacaactcgt
     6901 tcaatctctt ctgcctgata ggtattgcta tcgctggctt ggtctgtctc cagaatgtgc
     6961 tctccaaaat aaatcccacc tatcaactcc cgtaccatga ccaaatccac accgtccagc
     7021 cggtcggcct tcagaggaga atagtctttc aggctatcaa aaattttcac cggacggata
     7081 ttggcaaaga gacccaattc cttccgaagt tgaagcagac cttgttctgg tcgaacagct
     7141 gcatcatcat actgagggct accgatagct gctaacaaaa tcgcatccgc ctgacggcag
     7201 gcttgtaagg tagcatttgg tagaggatgg ccagccgcat caatcccagc gccaccaaaa
     7261 gctctctcct caatctcata atcaaatcct acttgcccag caacagcctc gaggactgct
     7321 aggccagcct ccatgatttc aggaccgatt ccatcaccag ccaaggctac aatttttttc
     7381 gtcatattcc ctcctagatg tgtggcaagt ccttctctga tagtttctta tctatctgtc
     7441 cactattttc tttttgcacc atgacattag catggatata ggcaatggca gaagccttca
     7501 ccacgtcaaa gtcaatacca gaggcattaa agattgtttc cgttgccttg ttttctacag
     7561 cgacaagcac acgcgcctgg gcatcaatcc catcggtgat ggcgtcaata tggtagcgtt
     7621 ccaagctaat ctcttggtgg aagaattgat caatggcatt aaagacagcc tcaaccgaac
     7681 ccttgcctga ctccacaaca accacttctt cctcatcctg attgatgaag gtaacttgag
     7741 cctgaatact tccgtcagac tgactatcca aacgcaaatc tttaaattgg aaaccatcac
     7801 gattgctgat ttctgtccct gctaccaagg cacgaatatc cgcatccgta atcttttcct
     7861 tcttgtctgc cagttttttg aaacaagcaa acaagctagc aacgtctcct tcttcaaaat
     7921 acaggtctag ttctttcagc ttttctacaa aagcatggcg accagatagt ttacccagtg
     7981 gcagagaatt tttcttgacc cctaccaact caggtgtaat gatttcataa gtcaatggat
     8041 ttttcaagac accatcctga tggataccag attcatgtga gaaggcattg ccacctacca
     8101 ccgccttatt gcgtggaata gcaattccag agaagcgaga gaccaattct gaagtgttga
     8161 gggtttcctt tagaacaatc ggactagtca catcatagaa atctttccga atttcaaggg
     8221 caacagctac ttcttcaagg gccgcattcc ctgctcgctc accgataccg ttgatggtgc
     8281 cctcgacacg accggcaccg tttttaatgg ccgatagggt attggcaact gccataccta
     8341 ggtcattgtg gcaatgcgga ctgaaaataa tctcacggtc cgatttgacg tttgttgtca
     8401 agtaacggaa aatttctccg tagtgctgag gagtcgtaaa gccgactgta tctggaatgt
     8461 tgatgtaggt cgcaccagca tctaccgctg tctgcaccac ttcaagcaaa taatccaact
     8521 cagtccgcgt cgcatcctct ggcgagaact cgaccacctc aaaacgctca cgtgcatagc
     8581 tgacatgttc agtgatttgc gccaaaattt cttctttggt cttttgcagt ttaaattcac
     8641 ggtggatggg gctggtcgca atgaaaacat ggatctgcgg atacttggca tctttcagag
     8701 cctcataaca agcatcaata tctgatttta ctgaccgtgc caaacctgtc acagccgtct
     8761 tggtcatcgc tgccgcaatc tgacgaaccg cttcaaacga atcaggactg gccgctggga
     8821 aacccgcttc aatggcagaa atcccccatt tttccaactg cttggcgatg gtcacttttt
     8881 ctttaatcga aaaattcaca cccggcgtct gttcaccatc tcgtaaagtc gtatctaaaa
     8941 attcaatcac acgcataaga atacctttct aaataaccct aacgtcaata ttaggttctc
     9001 tttcttagtt ttcaaagtat actaagaaaa agaaaacacc tcgaggacta ttacctgcga
     9061 gatgttgtca acagtgcccg cttggtaagc caaacaggac taatgcattc tttcagcact
     9121 agcccgaccg tcgcaacaac aaagcaaaga attttgtagt gacaggtttc atttgacttt
     9181 ttcctttcag tattttaatt atttacaatg ataccctact tattttcact tgtcaacaga
     9241 aaaatggaaa ttttctgaaa aatccgaact ctctttaagc cttgcagtca acacttaatt
     9301 tgatttttca tatattccac atccttaata aaaaaagtcc ttcgcacctg aaccaaatag
     9361 tccttgcgaa gggcttttct ttctatcgct ttgttatgtc aggaagtaag caaactactt
     9421 tccttgttcg cgttccaatt tcgccttatc atccattcgg aagaatggat agtaaatggc
     9481 catgttaatc aaaatcgtaa cgatttgaat aaccgctcca ctaattctac cacctgaggc
     9541 taggaaaccc gcaattactg gtggcgttgt ccacgaaaca accgctccaa ttggttttgc
     9601 aaccagtccc caatccattg caaagtaagt gacaatggtt gttacaactg gtgctaagac
     9661 aaatgggata gccattttta tattcaaaat tgttggaaaa ccaaatagaa tcggttctgc
     9721 cacttggaaa ctaactggcg caattgataa ttttcctaaa gctttatttt cttgacttcg
     9781 agaaaggaaa aacgttaaaa tcgccaatgc catcacacag cctgctccac ctgaaaaaac
     9841 aaaattataa agaaattcat aaataatgat atgtggcaac tcttcacctg cttgataggc
     9901 tagacggtta gcttctgaat tttgtaataa aagtggttcc aaaatcgggc cagtgacttg
     9961 agccccgtga acaccaaaca tccaaaagaa acactgaata aagatcacaa ccaatattcc
    10021 cggcagtgtc tgtgtcacca gaccaagtgg tttcgcaaca gtatcagcga tcaaagcatg
    10081 aatattatca attcctgttg cttttagaag aatcagtatc atcaaccaaa gcgaaataat
    10141 aaacaaacct ggaataatgg ctgaaaatgc cttcgcaaca ttcggtggta cagtatcagg
    10201 cattttcaaa acaatgtttc ttttaacaaa gaaactgaat atctcagtgg atattaaggc
    10261 aacaatgatg cctacaaata agccagcagt tcctaaatat ttcaaaggaa aaccatttcc
    10321 tgcatcggat gataaaactg gggttaaaag cacaaatgac gctatagaaa gcactcctgc
    10381 agataatcta tcaacatcgt accgtttagc tagatgataa gcgacagcaa atgttactac
    10441 taagccagct atcccaaaag tagcgtcagt tgctttccat aaaaattctg aaatccctat
    10501 atcagaaagc caggctgaaa acgcaggaat tggaaaatct gcaattaaca taaaaactga
    10561 gccaacaata atgagaggca ttgcagtagc catcccgtca cgcaaggcaa ttaaatgtct
    10621 ttggttgcta atttttgcag caaacttttg aaccgattga acattcataa tagtctcctt
    10681 aatatattat tactatattt tatcgaataa tagaacgagc caacattgtt aactggtgac
    10741 gcttccctct aatcgcttga cctaaagcaa aaatattttc aggaagtgct attccattaa
    10801 aagaatcccc aatatgttga atatctgcac cacaagcttt agaggtcaat gcaattttct
    10861 taattgtatc ggtatccgac gagtcctgac tagtaccatt agctgtcaaa actaaaactt
    10921 tattctctat agtctggtcc ttattatatg acctaacata gtctactatt tcctttaact
    10981 cctccgcaac aaaattcggt actgtatagg gcgctggtac tagcagaata tcaattcccg
    11041 aatgtataaa ctgcttagca atctctaaat tcatcacagg ctcatcaact ccagctccat
    11101 gcattttccc tgcgataatc aaaccagaaa aatattcttt tgccatagat attgcagaaa
    11161 atatggactg gttactgacc cctgtacctg ggtttcctgt caagcaaata aagtcgaagc
    11221 caagttctgt agcttttttt aatgttttcg gcgaaacaat tctacctatt gaaatttcag
    11281 cacgtgtttc aaccatattt gcttccaaat caacaggttc aagattgaca cctatcgccc
    11341 gcccgagagc tttttttatc caaacaattg gattttcttg aacatcctca gggatttccg
    11401 ctataagcgg atgaaacaag tccaatgcat tgaacattat caaatctgct cctgctgctc
    11461 tttccatttc tgcattagaa acaccactca gaaatgacga tctgctcgga acattttcag
    11521 ccatcactgt tcgtccctct gacgccagta ttgcttgttt cagttccata ggggaggccg
    11581 acaaaaaatc agatgctgaa taatcaagta accttttatt cataatttat cctttcaact
    11641 tgttataaca acgtatcgat tcatcaacat tatatatcca tttgtaagcg ttgtcaactg
    11701 tttttgttga cttttttcaa aaaaaatcta taatataacc aaagatgtta taataatatc
    11761 aaagaaagga tttccgacga tgagagacaa ttcaacaaaa tcaccactat attatcagct
    11821 attcgatagt ttggtggaat atattcaaaa agatttacaa ccacatgatc aattacctac
    11881 agaaaaagaa attggaactg attattccgt cagcagaaca accgtacgat tggccatgca
    11941 agagttagag aaacatggat acatttatcg aattcaagga aaggggtcgt ttgtatctag
    12001 tcttaaacaa gaaaatatca actcattttt ttcacttgaa ttaaaaaatc actatgatgg
    12061 gttagaaacc gcagactttg aatcccactt agtcagtttc acaaaagaaa acggttctct
    12121 agcattaaaa catctctttg gtctgtccaa acaaacaaat ctcgtccgca tacaattaga
    12181 acacctgctt cattcagaac ttgttgctac tgaaacaatt gtagcaaaag ctagctatat
    12241 gcctgaacta acgcaggaaa gtttggaatt tgaaggattg gactcgttgt tccaaaaaac
    12301 tggtgctatc actaaagctg ttgatgaaca ctattctctt gctagattta tgccaactag
    12361 ttcagaaatt gatgcattaa aaattacaaa atctattttt aaccaagata acgaattgat
    12421 gttaacttcc actagaatta tcaatatcca aaaactaccg taccgaaatt tta
//
LOCUS       DAZQCJ010000053        12441 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000053 DAZQCJ010000000
VERSION     DAZQCJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12441
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.054"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..258
                     /locus_tag="ACHU66_001334"
     CDS             <1..258
                     /locus_tag="ACHU66_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HFR3389615.1"
                     /translation="DVKNLRKLKRIELYEIMLAQSEEIDCLRNQLEKIKTELADRRIM
                     ISQSGSIAEASMKLTNIFEEAQKAADLYLYNVKNKIGDVHE"
     gene            251..841
                     /gene="lepB"
                     /locus_tag="ACHU66_001335"
     CDS             251..841
                     /gene="lepB"
                     /locus_tag="ACHU66_001335"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR3389616.1"
                     /translation="MNSSNLRGKKLPKTSDLQAVYQQAKYKKTFWETIRSTVFMLVVV
                     AAFAVLIAVLFLPILRIYGNSMKGTLNSGDIVVSVKSNDFESSDVVAFYYNNNILVKH
                     VIAEAGDWVDMDKQGNVYVNNQRLDEPYLANRDYSHTDIEFPYQVPENRIFVMGDNRK
                     ESIDSRNNAIGTVSNEQIVGKLVFKIWPLPELGWIE"
     gene            873..1472
                     /locus_tag="ACHU66_001336"
     CDS             873..1472
                     /locus_tag="ACHU66_001336"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389617.1"
                     /translation="MRDWLNLEKIQGWRRKYKKLVNIIAFLVVFATTYALILPAITLD
                     QEKAEQTAGIEVQETPVTTEISKLTEPMVTTQQTPIVGSSADEVQSPATEAVPDPAEQ
                     VSTESASKPQTDQERITEPTTIVHKTNDYEVTATFDASAQFPKDVEFNVRELDSQSEE
                     YRTHYEKTKEKLGVKNLYMPDSLIFIFHHRAKRSSLLPL"
     gene            1718..5860
                     /locus_tag="ACHU66_001337"
     CDS             1718..5860
                     /locus_tag="ACHU66_001337"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HFR3389618.1"
                     /translation="MIDKTVGTSLGSLPEAPFKNGYVFSHWINKDTGDRIDENTVITG
                     DLTAVAVFNNINIYTIDVTYFYHNVSKNQDITIDKETVQLEASDAPYQLTPPASTEVS
                     NKEDTTLPADAIYYPEKPLLEFTADQLERLDVADGVDDNHISERVQYVPYNAEYDVIY
                     RLKNLTGSGYSDIQTVHVRGVLGSTVTPQILTYNYADFERADTAKIENTSGQKLYVYY
                     TRKTFTLSYNSNGGSNIPQQQGLYDSQIKISTTTPTKEGYTFEGWYDNPGLTGNKITG
                     SLTLKKDTTLYAKWTPNRVRYIIGYYKEVYDNNTGTTSYVYDSSAAGWGLVGTTVKGT
                     DSNIGPPPPTQTTIGSIGYEKDVTKSAASSIEIAADGTSVLKVYYKLKRYTFEFFLND
                     NNANITINGKTYSDRNRYMIRDVVLGQDVSKLWPASITNPKEVYDDKVYYDSYGRPIR
                     YFFDYWIFGNLLFKTDRFEVVEPMLPTSGTTSKFTAYWNTQGTQANVEYWLQQPDGSY
                     KKSDIYSQSFLKTAGLLPKEIFGYSYYTDASFTEAGIRYYGTQGNTYRFYYKRQAFKI
                     DYYYNSTGLDSKSNILFESDINTATYNYQPPRSTGVDADYTWGGWYTDSLLTEKYNFN
                     KMPANNLVLYAKWIAPKFKVTFDINGGNGTAPVDQSVEKYKYAKAPADPTREHYNFLG
                     WSTEDGKLYSWSDQVTKDVKLTAKWELKPLTYTVRYLEAGTNTQLAADKVITSPALVL
                     NQAITEKALGITGYRPDKSSQTINLNFRGNEITFYYTPKVKDVVYTIKYVLKDNPAIE
                     VAPTVTRKVDGSIIRAKEAAADVNKQHLQSQSGVTAEMLEMDYYPTVNTQSLILTSND
                     ANNVIVFEYVGFDTQTFTVNYLDMDGKKIDGQEPLVVYRKKPGSFVVDHKTINGYTYD
                     HSLDSENAADKTVYRVSKGGHITIDLYYKKNLTLTALDKSKVYDGTALSSSGLSDLNP
                     AYSVDLKAGDKLVAIGYDGSQTNAGSSATTPKLANISNALGADRTNFYHITYQAGNLR
                     VDQQPVVVVINGEKKSKIYDGKAEAVGYKVMEIRDTSGLYKETDIHFNGSSSEQSVKK
                     KDAGIYPLLLNNRFTNTNQNFQVDFLISDGELRIDRRQLTITSDSASKDYDGSELRAD
                     NITVSVPAGVDYTGFVDGEGVVPTFLGGPTEPGFMPNYFTYQPKDGTNLANYELETQF
                     GQLKVKEVIKIQKTDLAWQALSGGKFELTKWDGLNWAQVDGAQEFAITSKDGSNIPVG
                     LEPGRYRLQELAAPDGFIILDSYIYFTIKDNFNEDQTMSFYTVTLSDEMGNDATPERA
                     QLERTTGDASHRIKVANEQGRALPSTGGSGQKWFILSGLVLMVTSYLIHLFVQRNRKM
                     RS"
     gene            5958..7394
                     /locus_tag="ACHU66_001338"
     CDS             5958..7394
                     /locus_tag="ACHU66_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFR3389619.1"
                     /translation="MNKFKSLFMTVLAVLAMVLPTTVASAQEVDTNAGGTGTITVRNA
                     SQSQKYTIYKLFDATVTEDGSGISYKVPTGKILPADNPYFAVDSKGNITAKEGATVSS
                     EAFAAWAKNFGTEVTSATANDNSLVFTHLTFGYYFVTSSLGAVLTVDSTTPNATVIDK
                     NTTNPTIPDSNNGGGKKILSNGATTSETTAKIGDTINFQIKFNATNYVTKDRQTKQIV
                     SYAIEDSPTALAIDQNSVNVKVDGVDITAKISKTFDATGKMNLVLTWADAAASNKTIY
                     NSPAEVIITYSAVVTKDAKEGEATNSATIGYNTIDNPTTPPTPVDPDKPTETTKVTTH
                     RFTLKKTNNVGETLTGAEFKLYDAANNGTEIKVVKESDGVYRVAQADEQGVVIEAGEV
                     VIKGLKHSTTYYLEEMKAPNGYNILTERQSIEVKENNTAQANIVNKKGGVLPSTGAIG
                     ATLFYLVGSILLLVALVYTISKRRMNNI"
     gene            7508..8377
                     /locus_tag="ACHU66_001339"
     CDS             7508..8377
                     /locus_tag="ACHU66_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFR3389620.1"
                     /translation="MIKWLKKKGPSFIMPLIFILGIGLLAYPSVSDYWNSFHQSEAIM
                     SYSDTVSDMTADQYKEIIDSARQYNQEKPLNWNVTASDIEAYNQELNFNKDGIMGYIE
                     IPKIDVKLSIFHGTDETVLETSIGHLEGTSLPVGGLGTHSVLSGHRGLPSARLFSDLD
                     KLREGDIFTIHVLNETLTYKVDQIRVVEPSDLSALTIEPQKDLLTLVTCTPYGINTHR
                     LLVRGYRIENVNGSALVTSDAIQIKAIFIAPFIATPILFVILIYIFITTSKTFRSWNR
                     NQVLDDFLVPKKF"
     gene            complement(8491..8768)
                     /locus_tag="ACHU66_001340"
                     /pseudo
     CDS             complement(8491..8768)
                     /locus_tag="ACHU66_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018958.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            8939..10288
                     /gene="murD"
                     /locus_tag="ACHU66_001341"
     CDS             8939..10288
                     /gene="murD"
                     /locus_tag="ACHU66_001341"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR3389621.1"
                     /translation="MKMIDIVKNKKVLVLGLAKSGESAARLLDKLSAIVTVNDGKPFE
                     ENPTAQSLLEDGIRVICGSHPLELLDEDFALMVKNPGIRYDNPMVEKAIGKGIPVWTE
                     VELAYLVSDAPIVGITGSNGKTTTTTMIAEVLNAGNKPAKLCGNIGYPASSVAQTATA
                     EDTLVMELSSFQLMGTESFRPHIAVVTNLIASHIDYHGTFEDYVAAKWMIQGQMAAED
                     FVVLNFNQKEAKELAEQTKATVVPLSTLEVVDGAYLADGKLYFKGEYIMDADQIGVPG
                     SHNVENALATIVVAKLLGVDRQAIQESLSAFGGVKHRLQFVGEINGVSFYNDSKSTNI
                     LATQKALSGFDNSKVILIAGGLDRGNEFDELVPDLVGLKKMVILGQSAPRVQRAADKA
                     EVETIEALDIADATRKAFAIAEKGDIVLLSPANASWDMYANFEVRGDVFLQTFEELK"
     gene            10291..11355
                     /locus_tag="ACHU66_001342"
     CDS             10291..11355
                     /locus_tag="ACHU66_001342"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HFR3389622.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLIPRFLEEGWEVHYIGDGNGIEHE
                     QVVKSGLDVHFHSISTGKLRRYFSFQNMLDVFKVGFGVLQSVAIIAKIRPQALFSKGG
                     FVSVPPVIAANLLRVPVFIHESDLTMGLANKIAYKFATTMYSTFEQPASLTKVKHVGA
                     VTKVGKTNDKVTPIQLPEILSHFDKSLPTLLFVGGSGGAKVFNDLISQNSAALTERFN
                     IINLTGDSSLNQLDKNLYRVDYVTELYQPLMDLADVVITRGGSNTLFELIAMQQLHLI
                     VPLGRQASRGDQIENALYAEKKGYSKQIDESQLTFASLLVEVDELLKNKEFYIQNMTN
                     SNEIQSVDSFYNLLREDMGR"
     gene            11361..>12441
                     /locus_tag="ACHU66_001343"
     CDS             11361..>12441
                     /locus_tag="ACHU66_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFR3389623.1"
                     /translation="MTEKDSNVEESVLEVEQASQVELDSEQISPAEKESVLAEEKGLS
                     TDVSTDVDIPEMTAPDDEKSAFFEQWKARHQAYLAHKDAAEIQAVDEGQTEQENPEAK
                     KSKRVLFQGIERKTESLKSKKETGEKVQTLKVDIPSKVVWKAIPVLVTSLLLAALALY
                     FISPTSKKKQIEVVGNERLTAEQVENYSLISPDDYNVTVALHADAYAKNIKKNSSSVE
                     TATIKFQFPATFTIHIKEYAIIGYIQQQSQWYPVLSSGEIGGEPISQDSMPEGYTTIN
                     LSDKELIKELAIELGKIDAGIRSAIQTINLTPSKVTVDLLTFNMRDGNTVLVPLSEIS
                     QKVSYYTKIAAEVTVPTTIDMEVGIY"
BASE COUNT         3991 a         2252 c         2716 g         3482 t
ORIGIN      
        1 gatgtaaaaa atcttcggaa attaaagcga attgagttat atgagattat gttggctcaa
       61 agtgaagaga ttgattgctt gagaaatcaa cttgagaaga ttaaaacaga gttggctgat
      121 agaagaatta tgattagtca atctggctcc atagcggaag cttcaatgaa actgactaat
      181 atttttgaag aagcgcaaaa agctgcggat ctatatctct ataatgtgaa aaataaaatt
      241 ggtgatgttc atgaatagtt caaacctcag gggaaagaaa cttccgaaaa cttccgattt
      301 acaagcagtt taccaacaag ctaaatataa aaagaccttt tgggaaacca tccgtagtac
      361 tgtatttatg cttgtagttg tagctgcttt tgctgtgctg attgctgtcc tttttcttcc
      421 cattctaagg atttacggga actcaatgaa agggaccctg aacagtgggg acatcgttgt
      481 gtccgtaaaa agtaatgatt ttgaaagcag tgatgttgtt gctttttatt acaataacaa
      541 tattctggtc aagcacgtca tcgctgaagc tggggattgg gtggatatgg ataaacaagg
      601 gaacgtttat gtcaataatc aaagattgga cgagccatat ttggcaaata gggattatag
      661 tcatacggat attgagtttc cttatcaggt accggaaaat cgaatctttg tcatgggaga
      721 taaccggaaa gagtctattg attcgcgaaa caacgctatt ggtacagtct ctaatgaaca
      781 gattgttgga aagttggtat ttaagatttg gccactgcct gaattgggat ggattgaata
      841 aatgaaagtg aaaaaataga aaggaggatc ttgtgagaga ctggttaaat ttagaaaaaa
      901 tccaaggttg gcgacgaaag tacaagaagc tcgtgaatat cattgctttc ctcgtagtat
      961 ttgcaacgac ctatgctttg attctacccg ctatcacatt agaccaagaa aaggctgaac
     1021 aaactgctgg gattgaagtg caagaaacac cagtaacaac ggaaattagt aaattaacag
     1081 aaccgatggt gacaactcag caaactccga tagtgggtag ctctgctgat gaagttcaat
     1141 ctcctgcgac agaagcggtt cctgaccctg ctgagcaagt gtcaacagag tctgctagca
     1201 aaccccagac cgaccaagaa cggattactg agccaactac catagttcac aaaacgaatg
     1261 attatgaggt gactgcaaca tttgatgcga gtgctcaatt tcccaaagat gttgagttta
     1321 atgttagaga actagatagc caatcagaag aatatcggac acattatgaa aaaaccaagg
     1381 agaagcttgg agtaaaaaac ttgtatatgc ccgattcttt gatattcatt tttcatcaca
     1441 gggccaagag gtcgagcctg ctgcccctat aaagattacg attagaaaca aggaattagt
     1501 tcaacggcca gatgatagta aactgaaaat tgtccacttt gaagagaata gccaaataga
     1561 agttttagag gccgaaacga aggaaaatga acaacaaatc tccgagatca gttttgacgc
     1621 tagccatttt tctgtttatg gagatatttt agcaaactat tataccgtca aatttaccta
     1681 cactgacaca gcgggtcaag aacaagtcat tacgagtttg attgataaga ctgtggggac
     1741 ttcacttggt agtttgcctg aagctccttt taaaaacggt tatgtatttt ctcattggat
     1801 taataaagat actggtgatc ggattgatga aaatacggtg ataactggag acctgactgc
     1861 tgtagcagta tttaataata tcaatatcta caccattgat gtcacgtatt tctaccacaa
     1921 tgtttctaaa aaccaagata ttacgattga taaggaaacg gtgcaactgg aagcaagtga
     1981 tgctccatat caactgacac cacctgcttc tactgaggtg agcaacaaag aggatacaac
     2041 tttaccagca gatgctattt actaccctga aaaaccactt cttgagttta cagcagatca
     2101 actagagcga ctggacgtag cagatggagt ggatgataat catattagcg aaagagtaca
     2161 atatgttcct tacaatgcag agtatgatgt catttatagg ctaaagaatt tgacggggtc
     2221 aggttattct gatatccaga ctgttcatgt ccgtggggta ctgggttcaa ctgttacacc
     2281 gcaaattcta acctacaact atgctgactt tgaacgtgct gatacagcaa agattgagaa
     2341 tactagcggc caaaaattgt atgtttatta tacacggaaa acctttactc tttcttataa
     2401 ttccaatggc ggtagtaata ttccccaaca acagggtcta tacgatagcc agattaagat
     2461 ttctacaact actccaacaa aagaggggta tacatttgaa ggttggtatg ataatcctgg
     2521 actgactgga aacaagatta caggctctct caccttaaag aaagatacaa ctttgtatgc
     2581 gaagtggaca ccgaacaggg ttcgctacat cattgggtat tacaaggaag tctatgacaa
     2641 caacactgga acaacctcct atgtatatga tagttcggca gctggttggg gtctggttgg
     2701 gactactgta aaaggaacag attcaaatat tggacctcct ccgccaaccc aaacaaccat
     2761 tggcagcatt ggctacgaga aggatgttac taaaagtgct gcttcaagta ttgaaattgc
     2821 agctgatgga acatctgttt tgaaagttta ctataaactg aagcgctaca cttttgaatt
     2881 tttcttaaat gataacaatg ccaatatcac cataaatggt aagacctact ctgataggaa
     2941 taggtacatg atacgtgatg ttgtgcttgg tcaagatgtc tcaaagttat ggccagcttc
     3001 tataacaaat cctaaagaag tatacgatga taaagtctac tatgatagct atggtaggcc
     3061 gataagatac ttttttgact actggatttt tgggaatcta ctcttcaaaa cagatcggtt
     3121 cgaagttgtt gagcctatgt tgccaacgag tggaacaacc agtaagttca cagcatattg
     3181 gaatacccaa ggtacacagg ctaacgttga atattggtta caacaaccgg atggttctta
     3241 taagaagtct gatatatata gtcaatcttt cctaaagact gcaggactgc taccgaaaga
     3301 aatttttgga tattcttatt atacagatgc tagttttact gaagcaggca ttaggtatta
     3361 cggaactcaa ggaaatacat atcgtttcta ctataaacgc caagccttca aaattgacta
     3421 ttattacaat agtactgggt tagattcaaa gagtaacatt ctctttgaga gtgacatcaa
     3481 tacagcaact tataattacc aaccaccacg ttctactgga gttgatgctg attatacatg
     3541 gggtggatgg tatacagatt cgttattgac agagaagtat aactttaata agatgcctgc
     3601 caataacttg gttctctatg ccaagtggat tgctccaaaa tttaaggtta cttttgatat
     3661 caacggtggt aatggaactg cccctgtcga tcaaagtgtt gaaaaataca aatacgctaa
     3721 ggcccctgct gatccaacga gggaacatta caatttcctt ggttggtcta cagaagatgg
     3781 gaaactctat agttggagcg accaagtaac caaggatgtc aaattgactg cgaagtggga
     3841 attgaaacca ttaacttata ctgttcgcta tcttgaagca ggtacaaata cgcaattggc
     3901 tgctgataaa gtgattacga gtccagcgct tgtattgaat caagctatca cagaaaaggc
     3961 attgggaatt acaggttatc gaccagataa gagtagtcaa acaattaacc tgaattttag
     4021 agggaacgaa ataactttct actatacacc gaaggtaaaa gatgttgttt acaccatcaa
     4081 gtatgtcttg aaggataacc cagctattga ggtagcacct acagttactc gtaaggttga
     4141 tggaagtatc atccgtgcga aagaagctgc tgcagatgtg aataaacagc atttgcagag
     4201 tcagtctggt gttactgctg aaatgctgga aatggattac tatccaactg taaatactca
     4261 aagtttgatt ctaacttcta atgatgcaaa taatgtgatt gtttttgaat atgtcggatt
     4321 cgatactcaa acctttacgg taaattattt ggatatggat ggtaagaaaa tcgatggtca
     4381 agaaccattg gtagtttacc gaaagaaacc aggtagtttc gtggtagatc ataagactat
     4441 aaatggttat acctatgatc attcgttaga cagtgagaat gcagctgata agacagtata
     4501 ccgtgtaagt aagggtggtc acataaccat tgatctctat tataagaaaa acctaacact
     4561 gaccgcatta gataagtcca aagtttatga tggcactgcc ctatcaagtt cgggtctatc
     4621 tgatctaaac cctgcttaca gtgtagacct gaaagcagga gacaaattag ttgccatcgg
     4681 ctatgatggt agccaaacca atgcgggtag cagcgcaacg acacctaaac ttgctaacat
     4741 cagcaatgct cttggtgctg accggacgaa tttctatcac ataacctatc aagcgggtaa
     4801 cttgagagtt gaccaacagc cagtcgtggt tgtcatcaac ggtgaaaaga aatcgaaaat
     4861 ctatgatggt aaagcggaag cggttggtta taaggtgatg gagataagag atacaagtgg
     4921 tctctataaa gaaacagata tccattttaa tggttcaagt tcagagcagt cggtcaagaa
     4981 aaaagatgca ggaatttatc cgctcctttt aaataacaga ttcacaaata ccaatcagaa
     5041 tttccaagta gatttcttga tttctgatgg ggaattaaga atagatagac ggcaactgac
     5101 cataacatct gattcagcaa gcaaggatta tgatggtagt gagctccgtg ctgacaatat
     5161 cactgtatca gtacctgctg gtgttgacta cacaggtttt gtcgatggag aaggagttgt
     5221 tccaaccttc ctaggcggac caaccgaacc tggcttcatg cccaattact tcacctatca
     5281 gcctaaagat ggaactaatc tggctaatta tgagttggaa acacaatttg gtcagttgaa
     5341 agtcaaagaa gttatcaaga ttcaaaagac tgacttggct tggcaggctc tgtcaggtgg
     5401 taagtttgaa cttaccaagt gggatggcct caattgggct caggtagatg gtgcacaaga
     5461 atttgcgatt accagcaaag atggcagcaa tattcctgtg ggcttggaac ctggtcgtta
     5521 ccgactccag gagttggcag caccggatgg cttcattatc ctagatagct atatctactt
     5581 caccatcaag gataacttca atgaagatca gacaatgtcc ttctacacgg tcaccctatc
     5641 tgatgaaatg ggcaatgatg ccactccaga acgggcccag ttagaaagaa caacaggtga
     5701 tgccagtcat cgtatcaagg ttgctaatga gcaaggccga gctctgccaa gcactggcgg
     5761 tagtggacag aaatggttta tcctttcagg acttgttctc atggtcacct cctatctgat
     5821 acatctgttt gttcagcgta atcggaaaat gaggtcttag aactgagttt ccgcttgctt
     5881 taatttcgtg tcgcaatgcg agttgcagga tttcatgtct gcctctccat aataaaaaaa
     5941 taaaaaagga aaaaatcatg aataaattta aaagtctctt catgaccgtg ttggcagtgc
     6001 tagctatggt cttgcctacc actgtcgcta gtgcgcaaga ggtagataca aatgcaggtg
     6061 gtacaggaac aattactgtg cgcaatgctt cgcaaagcca aaaatatacc atctataaac
     6121 tctttgatgc tactgtaaca gaagatggtt caggtatttc ctacaaggtt ccgactggaa
     6181 aaatacttcc agcagataat ccctactttg ccgtggacag caaaggtaat attactgcaa
     6241 aggaaggtgc tactgtctct tcagaagcat ttgcggcttg ggcaaagaat ttcggtacag
     6301 aagttacaag tgctacagcg aatgataact ctcttgtgtt tacacatttg acatttggtt
     6361 actattttgt aacatcatca cttggtgcag tgcttacagt agatagtaca acaccaaatg
     6421 caactgttat cgacaaaaat accactaatc caaccattcc agattcaaat aatggtggcg
     6481 gtaagaaaat tcttagcaat ggtgctacaa ctagtgaaac aactgcaaaa attggcgata
     6541 ccatcaactt ccaaatcaag tttaatgcta ccaactacgt tactaaagat agacaaacca
     6601 agcagattgt ttcttacgct atcgaagata gcccaacagc cctcgcaatt gatcaaaatt
     6661 ctgtcaacgt taaagtggat ggtgtggata tcacagcaaa aatttcgaag acttttgatg
     6721 caacaggcaa aatgaatctt gtccttacat gggcggatgc ggcagctagt aataaaacca
     6781 tctataactc tccagctgaa gttattatca cctactcagc agtggtgact aaagatgcca
     6841 aggagggaga agcaaccaac tcagcgacta ttggttacaa cacaattgat aatccaacaa
     6901 ctcctccaac accagttgat ccagacaagc caactgaaac aacaaaagtg actactcacc
     6961 gtttcacttt gaaaaaaaca aacaatgtag gtgaaacttt gacaggtgca gagttcaaac
     7021 tctatgatgc agccaacaat ggaactgaaa tcaaggttgt caaggaatct gacggagtgt
     7081 atcgtgttgc gcaggctgat gagcaaggtg ttgtcatcga agctggcgaa gttgtgatta
     7141 aaggtctgaa acatagcacg acctattatc ttgaagaaat gaaagcaccg aatggttaca
     7201 atatcttgac agaacgtcaa tccattgaag tgaaagagaa taacacagca caagcaaata
     7261 tcgtcaataa aaagggtggt gtcttgccaa gcactggtgc gattggagca actttattct
     7321 atctagttgg ttcaatccta cttttggtag cattggttta tactatttca aaacgtcgta
     7381 tgaacaatat ctaattgtcc atacaatacc taatatttag aatctatctt cggaagtgca
     7441 aaagcaagag tggaagtatt tccgaagata tttctttggt ctcaacacaa ggaaaggagg
     7501 ggatttcatg atcaaatggc tcaaaaagaa aggtccgtct tttatcatgc cattaatttt
     7561 tatattgggg attggactat tggcttatcc atcggtcagt gattactgga attccttcca
     7621 ccaatcagag gccatcatgt cctattctga cacagtatca gatatgacag ctgaccaata
     7681 caaagaaatc attgatagtg cgcgccagta taatcaagaa aaacccttaa actggaatgt
     7741 gactgcatcc gacattgaag cctataatca ggaattgaat ttcaataaag atggcatcat
     7801 gggctatatt gagattccta aaattgatgt caagttatct atatttcatg ggacagacga
     7861 aacggtttta gagacttcta ttggacatct agaaggaaca tcgttgcctg taggaggttt
     7921 gggaactcat agtgtacttt cagggcatcg tgggctccca tctgcccgtc tgttttctga
     7981 tttagataag ttgagagaag gcgatatatt taccattcat gttctcaatg aaacactaac
     8041 ttacaaagtg gaccagattc gagtggttga gccatcagat ttatcggctc ttaccataga
     8101 acctcagaaa gacttattga ccttggtaac ctgtacaccc tacggtatta atacacatag
     8161 gctattagtt cgtggctatc gtattgaaaa tgttaatggt agtgctttgg ttacctctga
     8221 tgctatccaa attaaagcaa tttttattgc tccatttatc gcaactccaa ttctatttgt
     8281 aatcttaatt tatattttta taacaactag taaaacattt cgttcttgga ataggaatca
     8341 agtgttggat gattttttag ttcccaagaa attttgaact attgaaaatg tttcaaagtc
     8401 gatttgactc tggaacattt ttctattata gacattagac aatatggctg aatgaggtaa
     8461 ttgttcaact tctaacctcc tgtcaactca aatcaattgc ctagaccgac gattgaaacg
     8521 acttctttct agtaacggac cacgaggaaa gaggtggcag atattgtaga aatgtcgttg
     8581 agatgtcata cctagttcag cttgtaacaa aagcaagaca aggagtaatc tatctgaaag
     8641 cctacattac gccgaagtta aaaggaatct gggcaatagt tttggtagaa atgatgagaa
     8701 atttgtgata attggcgtac attccattgc aaatgatgag atttagcagt atactgtaag
     8761 tggctcattt ggactacccc tctgtatgtt tcgtcgctta cagtatagtc catttgggct
     8821 ttttattgta ttttgaaatg aactagcgct acgggtagag tttacttttc tgtcctctgg
     8881 cttgaaaaag gtcttcgtaa agggtaaaat aaggtgttag aaagtaaagg taattgagat
     8941 gaagatgatt gatattgtaa aaaataagaa agtgctagtg ctaggtttag ctaaatcagg
     9001 ggaatctgca gcccgtctcc tagataaatt gagtgctatc gtcactgtta acgatggaaa
     9061 gccttttgaa gaaaatccga ctgctcaatc tttgttggaa gatggtatcc gtgttatttg
     9121 cgggagccat cccttggaat tattggatga agatttcgct ttgatggtaa aaaatccagg
     9181 aattcgctat gataatccta tggttgaaaa agcaattggc aaaggaattc cagtatggac
     9241 agaggttgaa ttggcctact tggtatcaga tgctccaatc gtggggataa caggttcgaa
     9301 cggcaagaca acgacaacga ccatgattgc agaagtcttg aatgcaggaa ataaacctgc
     9361 aaaattatgt ggaaatattg gctatccagc atcttcggtc gctcaaactg cgactgcaga
     9421 ggataccttg gtgatggaat tatcatcttt ccagttaatg gggacagaat ctttccgccc
     9481 gcatatcgcc gttgtgacca acctcattgc cagtcatatt gattatcatg ggacttttga
     9541 agactatgta gcagctaaat ggatgattca gggtcagatg gcggctgagg attttgtggt
     9601 gctcaacttc aaccagaaag aagctaagga attggctgaa cagaccaaag caactgtagt
     9661 tcctctttct acgctggaag tagttgatgg agcttacttg gcagatggaa aactctattt
     9721 caagggtgaa tacatcatgg atgctgacca aattggtgtt ccaggttcac acaatgttga
     9781 aaatgccttg gcaacaattg tggttgcaaa attgttggga gtagatcgcc aagcgattca
     9841 ggaaagtttg tctgcctttg gaggtgtcaa acaccgcttg caatttgtag gtgaaatcaa
     9901 cggggtttcc ttctacaatg atagtaagtc taccaatatc ttggcgactc aaaaagctct
     9961 ttcagggttt gacaatagca aggtcatttt gattgcaggt ggcttggacc gtgggaatga
    10021 atttgatgaa ttagtccctg atttggtcgg actaaagaaa atggtgatcc ttggtcaatc
    10081 agctccccgt gttcaacgtg ctgcagataa ggcagaagtg gaaacgatag aagctctcga
    10141 tattgcggat gcgactagaa aagcctttgc cattgcagaa aaaggagata ttgtcctttt
    10201 aagtccagcc aatgcaagct gggatatgta tgccaatttt gaagtgcgtg gcgacgtctt
    10261 cttacagacc tttgaggagt tgaaataatc atgaaaaaaa ttgtttttac aggcggaggg
    10321 actgttgggc acgtgacgct caatctcctt ttgattccgc gatttttgga agaaggctgg
    10381 gaagtccact atattggtga tgggaacggt attgagcatg agcaggttgt gaaatcaggt
    10441 ttagatgtcc attttcattc tatttcgact ggaaaattgc gccgttattt ctctttccaa
    10501 aatatgttgg acgtgtttaa agttggtttt ggtgttcttc aatccgtggc cattattgct
    10561 aaaattcgtc cgcaagctct tttttcaaaa ggtgggtttg tatcggttcc gccagttatt
    10621 gctgctaatt tgctgcgggt tccggtcttt atccatgaat cagacttaac aatgggactg
    10681 gccaataaaa tagcctataa atttgcgaca accatgtata gtacttttga gcaacctgct
    10741 agcttgacta aagtaaaaca tgttggtgcg gttacaaaag tggggaaaac gaatgacaaa
    10801 gttacaccaa tccagttgcc agaaattctc agtcacttcg ataaaagtct gccgacccta
    10861 ctgtttgtag gtggttcggg cggtgctaag gtctttaatg atttaatcag tcagaatagt
    10921 gctgctctga ccgaacgatt caatattatc aatttgacag gggatagtag tctcaaccaa
    10981 ttagataaaa acctatatag agttgactat gtgacagaac tttatcaacc gttgatggat
    11041 ttagcggatg tcgttattac tcgtggtggt tccaatactc tgtttgagtt gattgccatg
    11101 caacaactcc atttgattgt gcccctagga cgacaggcca gtcgagggga tcagattgaa
    11161 aatgctctct atgcagagaa aaagggatat tctaagcaga ttgatgaaag tcaattgaca
    11221 tttgccagcc tgttagtaga agtagatgag ctattgaaaa ataaggaatt ttatatccaa
    11281 aacatgacaa attctaatga aattcagtca gtagatagtt tttacaattt gcttagagaa
    11341 gatatgggaa ggtaggtcct atgacggaaa aagattcaaa tgtagaagaa tcagtgctag
    11401 aagtggaaca ggcatctcaa gtagaattgg attcagaaca aatctcacca gccgagaaag
    11461 aatctgtatt agccgaagaa aaaggacttt ctacggatgt ttctacggat gtagacatac
    11521 ctgagatgac tgctccggat gatgaaaaaa gtgccttttt cgaacaatgg aaagcacgcc
    11581 atcaagccta ccttgctcat aaagatgctg cggaaattca ggcagttgac gagggacaga
    11641 cagaacagga gaacccagaa gctaaaaaga gtaaaagggt cctttttcag ggaatagaaa
    11701 gaaaaacaga gagtctgaaa tctaaaaagg aaacagggga aaaggttcaa actttaaaag
    11761 ttgatattcc ttccaaagta gtctggaaag ctattccggt tcttgtgacg agtttgttac
    11821 tggcagctct agccttgtat tttatttctc caaccagcaa gaaaaaacaa atagaggttg
    11881 tcggaaatga gcgactaact gctgagcaag tagaaaatta tagccttatc tctccggatg
    11941 attacaatgt gactgtagct ttgcatgcag atgcttacgc aaagaatatt aagaagaata
    12001 gttcgtccgt tgagacagcg acaatcaagt tccagtttcc agctaccttt accattcaca
    12061 tcaaagaata cgcaataata ggctacattc agcaacaaag tcagtggtat ccagtattgt
    12121 ctagtggaga gattggcggt gaaccgattt cacaggattc catgccagaa ggctacacaa
    12181 cgataaattt atccgacaag gaattaatca aggaactggc cattgaattg ggtaaaatag
    12241 atgcaggaat tcgttctgct attcagacaa taaatttaac gccaagtaaa gtgaccgtgg
    12301 atttgctgac attcaatatg agagatggaa atactgtatt agtcccgttg agtgagatta
    12361 gccagaaggt gtcctactat acaaaaatag cagcagaagt gactgttcca acaacaattg
    12421 atatggaagt tggtatttat a
//
LOCUS       DAZQCJ010000054        12378 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000054 DAZQCJ010000000
VERSION     DAZQCJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12378
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.055"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(92..880)
                     /gene="yghU"
                     /locus_tag="ACHU66_001344"
     CDS             complement(92..880)
                     /gene="yghU"
                     /locus_tag="ACHU66_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR3389624.1"
                     /translation="MTEYTKPLVWKWEDENDNRSGNRPTAGSRFEQALPKGEKSFQVY
                     SLGTPNGIKVAIMLEELKELGISEADYDLFLINIGQGDQFGSDFVAINPNSKIPAMVD
                     YSEQEPISVFESANILLCLAEKFDAFLPKTWAERTEVLNWLFWQTGAAPFVGGGFGHF
                     FHYAPTAQEYPINRYTMEAKRQLDLLDQLLATREYIAGNTYTIADIAIWSWYGRLVQG
                     QLYPGSAEFLDVDSYKHLKTWVEKIAQRPAVQRGLTVKYHSIDN"
     gene            complement(889..1179)
                     /locus_tag="ACHU66_001345"
     CDS             complement(889..1179)
                     /locus_tag="ACHU66_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389625.1"
                     /translation="MKVAEKEELYKYLSAAYNLPQEAFSEALREKILEVAGQLDKEEN
                     LYILAGHLSRFINAELTALTCRAPKELVQLAHYLQEVQNHYRYASLFPGKVK"
     gene            complement(1285..1974)
                     /gene="rplA"
                     /locus_tag="ACHU66_001346"
     CDS             complement(1285..1974)
                     /gene="rplA"
                     /locus_tag="ACHU66_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR3389626.1"
                     /translation="MAKKSKNLRAALEKIDSTKLYSVEEAVALAKETNFAKFDASVEV
                     AYNLNIDVRKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINGGWLDFDVVIATPDMMAVVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGIVQALIGKVSFDADKLVENFKAFHDVMAKAKPATAKGTYMTSVSITTTQ
                     GVGIKVDPNSL"
     gene            complement(2015..2440)
                     /gene="rplK"
                     /locus_tag="ACHU66_001347"
     CDS             complement(2015..2440)
                     /gene="rplK"
                     /locus_tag="ACHU66_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR3389627.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFITKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAASLEAAMRMIEGTARSMGFTVTD"
     gene            2595..2963
                     /locus_tag="ACHU66_001348"
     CDS             2595..2963
                     /locus_tag="ACHU66_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFR3389628.1"
                     /translation="MFESIIFLKIVAFLFIFLTLAISIIAVKFFRLQNRGWNFADIAF
                     PLYAIEFYLISDKAYYNSLLPQLVLALSLLAIGLCGFFLLKKKNFLYRRFFKVFWRAS
                     FILTFLMYLALVIAVFTLKS"
     gene            complement(3326..3712)
                     /locus_tag="ACHU66_001349"
     CDS             complement(3326..3712)
                     /locus_tag="ACHU66_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR3389629.1"
                     /translation="MKFDAVHHIAIIGSDYDKTREFYVEKLGFEQVDEHIRPEKNDIL
                     FNVKKGNLILEIFIKPDAPMRPAMPNPEHTGLRHLAFQVADVEACLEEFDRLDIRHEV
                     LRTDDFDGKKMAFFFDPDGLPLEIHE"
     gene            complement(3712..6081)
                     /locus_tag="ACHU66_001350"
     CDS             complement(3712..6081)
                     /locus_tag="ACHU66_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR3389630.1"
                     /translation="MRKTSKKGKATRRPTKAELAQQERVKNITLRVLGLLFIAFAASR
                     LGVFGVTSYNIFRLLFGSLAYLLLAGAFIYLLIPKVLREREGTISGFWLIVIGLLIEF
                     QAYLDWTYQGSDLFGHTLKLALSDLAKFQVTAFLGGGMIGSIFYLPVSFLFANVGSFF
                     IGLLVIAFGIFFVSPWSVYDVADGLAVAKDKLVETQVKRAELRTQKKAEREEKRQQEL
                     ARLEEERLRLEEEEESARALALVDRHVDLETGEILEEEPVQVPIISEYDHLDIEDEFP
                     IMLAEDDQPAQSNSASQTSEPNEFEKDEIDVQIDFKPKQRLAYKLPSIDLFAPIKAKS
                     QSNEKRIVRQNIKVLEDTFASFGIKVVVERAEIGPSVTKYEVKPAVGVRVNRISNLAD
                     DLALALAAKDVRIEAPIPGKSLVGIEVPNSEVATVPFRELWEQSKTDPDKLLEIPLGK
                     AVNGSVRSFNLARMPHLLVAGSTGSGKSVAVNGIISSILMKAGPDQVKFMMIDPKMVE
                     LSVYNDIPHLLIPVVTNPRKAARALQKVVDEMEKRYELFSQIGVRNLEGYNAKVEEFN
                     SRSEEKQIPLPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGIHMILATQRPSV
                     DVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKLLGRGDMLFKPIDENHPVRLQG
                     SFISDDDVEAIVGFIKDQADADYDESFDPGEVAEGDGDTGFGDTGGDPLFNEARALVV
                     ETQKASASMIQRRLSVGFNRATRLMEELEAAGVIGPAEGTKPRKVLETQ"
     gene            complement(6207..7565)
                     /locus_tag="ACHU66_001351"
     CDS             complement(6207..7565)
                     /locus_tag="ACHU66_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFR3389631.1"
                     /translation="MKTVVEANQFIQTEKGNVNPIFKPQAHLTPETGWINDPNGFIYF
                     RGEYHLFYQFNPYESVWGPMHWGHAKSKDLVNWEQLPVALAPDKAYDKDGCFSGSAIV
                     KDDVLWLMYTGHINNEDGTVSQVQNMAFSTDGIHFEKIEQNPVATADGLPEEVIANDF
                     RDPKIFEKDGHYYSVVATKHKDNVGCIVLLSSPNLTDWKFESIFLKGEANQGFVWECP
                     DYFEVDGQEYLIISPMRYQKDGNDFININSNIFVMGHVDWDKKVFVAESFKEIDHGHD
                     FYAAQTTEGPEGERVMIAWMHTWGRPLVTNDLDHKWYGQMTLPRILKKTENGLHQVLP
                     TSVFEAFKDIEIGQSIQGPSKLSLRLEDSLELKLGNDQDYLQFGYDKAKQEVYINRSS
                     LKIQQVGEEEWSTVRRAVTVQATELEVLVDTNCLEIFINDGQETLTSTFYVEGQIILN
                     AL"
     gene            complement(7583..9193)
                     /locus_tag="ACHU66_001352"
     CDS             complement(7583..9193)
                     /locus_tag="ACHU66_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR3389632.1"
                     /translation="MKHKFGKTVVTLLASTVLLAACGSKNTASSPDYELKDVQFPLEK
                     SVTLKFMTASSPLAPADPNDKLIFQRLEEETGVHIDWTNYQSDFGEKRNLDIASGDLP
                     DAIHNDGASDVELMNWAKQGVIVPVEDLIDKHMPNLKKILDENPEYRSMITAPDGHIY
                     SFPWIEELGEGKESIHTVNDIAWINKEWLNKLGLEMPKTTDDLIKVLEAFKTQDPNGN
                     GKADEIPMSFVNGNGGEDFKILFGAFGVGDNDDHIVVNNDGTVDFTADNEDYKEGVEF
                     MRTLQEKGLIDPEAFEQDWNTYLAKGSENLYGVYFTWDKANITGMNETYDILPVLAGP
                     DGTKHITRTNGMGFQRDRMVITSANKNLELTAKWIDAQYKPIQSVQNNWGTYGDETQQ
                     NIFEYDAATDSLKHLPLNGTAPGELRQKTEVGGPLAILDEYYGTVTTMPDDAKWRLDL
                     MHATYLDYVTNNDIYPRVFMEQEDLDKIAQVEADMNDFIYRKRAEWIVNGGIEEEWDS
                     YLKELESYGLSDWLAIKQKYYDQYKENQ"
     gene            complement(9393..10280)
                     /locus_tag="ACHU66_001353"
     CDS             complement(9393..10280)
                     /locus_tag="ACHU66_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR3389633.1"
                     /translation="MNTQMYTKFDKRMLIVNKILLAFLVLITVVPMVYILVASFMDPQ
                     ALVSKGISFNPKDWTVEGYQRVFSDKSILRGFFNSLFYSFSFAFVTVAISVMTAYPLS
                     KKDLVGKKWINAFLVFTMFFGGGLVPTYLLVKDLGMLNTVWAIIIPGAVNVWNIILAR
                     TYFQGLPDELVEAAVMDGANEFQIFWKIMIPLAKPIMFVLFLYAFVGQWNSYFDAMIY
                     IKDPDLEPLQLVLRKILIQSEPAKDMIGAQAAMNEMKRIAEMIKYATIVISSLPLIVM
                     YPFFQKYFDKGIMAGSLKG"
     gene            complement(10290..11210)
                     /locus_tag="ACHU66_001354"
     CDS             complement(10290..11210)
                     /locus_tag="ACHU66_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR3389634.1"
                     /translation="MKTSKLQQAGLVDRIKQQKLLLLMLLPGLVLTFIFRYIPMYGVL
                     IAFKDYNPLKGVLGSEWIGFEEFTKFLSSPNFGTLLANTLKLSVYGLLLGFLPPIILA
                     IMLNQLLSDKAKKRIQLVLYAPNFISVVVIVGMIFLFFSVGGPVNSILGMFGIEANFL
                     TDPDFFRPLYILSGIWQVMGWASTLYTATLVNVDPALIEAAKLDGANIFQRIWHIDLP
                     ALKPVMVIQFILAAGGIMNVGYEKAFLMQTSLNLTSSEIISTYVYKIGLVSGDYSYST
                     AVGLFNALINIILLIAVNKIVKRINDGQGL"
     gene            complement(11365..12354)
                     /locus_tag="ACHU66_001355"
     CDS             complement(11365..12354)
                     /locus_tag="ACHU66_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR3389635.1"
                     /translation="MEEQKSITMKDVARVAGVSVGTVSRVINNEPGIKESTLEKVNTA
                     IRELNYIPDVYARGMKKNKTETIALIIPTVWHPFFGEFAYHVEVELSKKNYKLLLCNI
                     SGPKRELDYLTMLQQNKVDGIIAITYSPIEDYLSSNIPFVSIDRVYENKDIACVSSDN
                     RAGAELAANTLIAKGGRHFAFIGGHNKTINETKKRRLYFEKRILEAGFPCHVLDLEEP
                     YDDFVGQVEEFLINNPQVDAIFTINDFTALDTLAVLEKLGRRVPEDVQVIGYDGIQLA
                     SEKSLALSTIRQPLESMAQEAVACLIDIIEKRERPLQVTLPISYLEGKTTKKF"
BASE COUNT         3428 a         2685 c         2497 g         3768 t
ORIGIN      
        1 ttttggattt tcacaaagtc taactctttc attataaact tttatcatat caaagtaaaa
       61 gaaaaaccca cccataaggt gggcaagtca attaattatc aattgaatga tatttcactg
      121 tcaaccctcg ttgtacagct gggcgctgag ctattttctc tacccaggtc tttagatgct
      181 tgtaagaatc aacatctaaa aattctgctg aacctggata tagttgacct tgcaccaagc
      241 gaccatacca agaccaaata gcaatatctg cgatggtata ggtatttcct gctatatatt
      301 ctcttgtcgc caatagctga tccaagagat ctagctgacg cttagcttcc attgtatagc
      361 gattaatcgg atattcttgc gctgtcggag cgtaatggaa gaaatgtcca aatcctccac
      421 caacaaatgg agcggcgcca gtttgccaga agagccaatt aagaacctct gtccgctctg
      481 cccaagtttt tggtaaaaag gcgtcaaact tttctgccag gcaaagtaga atattggctg
      541 attcaaagac cgaaatcggt tcttgctcgg aataatctac catagctggt attttggagt
      601 tggggttgat ggcaacaaaa tcggagccaa attggtctcc ttgaccgata ttgatgagaa
      661 agaggtcata atcagcttcc gaaataccta actctttcaa ctcctccaac ataatagcaa
      721 ccttaatgcc attcggcgtc cccaatgaat agacttggaa agacttctct ccttttggta
      781 aagcttgctc aaaacgacta cctgctgttg gacggttgcc agatcggttg tcattttcat
      841 cttcccactt ccaaacgagt ggttttgtat attctgtcat gttttctcct attttacctt
      901 tcctggaaaa agactggcat agcgataatg gttttgaacc tcttgaaggt aatgggctag
      961 ttgcaccaat tccttaggcg ctcgacaagt cagagctgtt aactctgcat tgataaagcg
     1021 acttaagtgt cctgccagta tatagagatt ttcctccttg tccagctgac ctgccacctc
     1081 caatattttt tcacgcaggg cttctgaaaa tgcttcctgc ggaaggttgt aggctgcaga
     1141 aaggtattta taaagctctt ctttctctgc tactttcatc ggataactcc ttttatgtaa
     1201 attgaatata gtataaatcg aagtcaagtg cttgtcaaga aaatctcaaa aaaaaagatt
     1261 gccctaagac aatctttcta ttgattaaag tgagttagga tcaactttga taccaacacc
     1321 ttgtgttgtt gtaatagata ctgaagtcat gtaagtacct ttagctgtag ctggtttagc
     1381 tttagccatt acatcgtgga aagctttgaa gttttcaacg agtttgtcag cgtcaaatga
     1441 tactttaccg ataagagctt gtacgatacc tgctttgtca gcacggtaag tgattttacc
     1501 accttttgac tcttcaactg ctttagcaac atccattgtt actgtaccag ttttagggtt
     1561 tggcatcaag ttacgtggac caagtacacg accaagacgt ccaacaacag ccatcatatc
     1621 tggagttgcg ataacaacgt cgaagtccaa ccaaccaccg ttgattttag caacgaggtc
     1681 atcttcacca acaaagtctg caccagcagc ttttgcttct tctgctttag caccacgtgc
     1741 gaaaacaaga acacgtgaag ttttaccagt accgtttggc aataccattg caccacggat
     1801 ttgttggtct gctttacgta cgtcgatgtt caagttgtaa gcaacttcaa ctgacgcatc
     1861 aaatttagcg aagttagttt cttttgcaag agcaacagct tcttctacgc tgtaaagttt
     1921 tgtgctgtca attttttcaa gagcagcacg caagtttttg ctttttttag ccatgttata
     1981 tgtctccttg taatcagata atcttgggtg ctacttagtc agtaacagtg aatcccatag
     2041 aacgagcagt accttcgatc atacgcattg cagcttcaag agatgcagcg ttcaagtcag
     2101 gcattttagt ttcagcaatt tcttgtactt gtgcacgagt aactgttgca actttagttt
     2161 tgtttggtgt acctgaacct ttttcaacac ctgcagcttt tttcaaaaga acagcagctg
     2221 gtggtgtttt agtgatgaaa gtgaatgatt tatcttcata cacagagata actactggga
     2281 tgatcatacc agcttggtca gctgtacgag cgttaaactc ttttgtgaat cccatgatgt
     2341 tgatacctgc ttgaccaagt gctggtccga ctggtggagc tggagtagct ttacctgcag
     2401 gaatttgaag ttttacgagt ttttcgactt ttttagccat gatataaaat cctcctattg
     2461 tggttttggc ggtaattaaa gatttttacc tcccacaaat atgtgtttta cacatacctt
     2521 ttcattatac acgaatttca aagaaatgca agctttaggt ttactttttt tcacattttt
     2581 tatagaatga atgtatgttt gaatcgatta tatttttaaa aatagtagcc tttctattca
     2641 tctttctaac cttggccatt tcgataattg ctgtcaagtt ttttaggttg caaaaccgag
     2701 gatggaactt tgcagacatt gcctttcctc tctatgcaat tgaattctat ctgatttcgg
     2761 acaaggccta ctataacagt ctactgcctc agcttgtcct agccctctca ctactagcga
     2821 ttggtctttg cggtttcttc cttttaaaga aaaagaattt tttatatcgc cgtttcttca
     2881 aggttttctg gcgagctagt tttatcttga cctttttgat gtatctagct ttggttattg
     2941 cggtcttcac tttaaaatcc taaaatcatc catcctttca tagatggcat aataaatccc
     3001 cattgcaggc gcagtgggga ttctttttta tactaaatca gacaaggcga cgtagatgta
     3061 tagtcgaatg aattagcttt cagacaagga actgaggtgc aggttgctag cacagcctag
     3121 tggctgtgct aggttggaga tataacttgc aaagcaagtc acttctgcag agtacggcaa
     3181 gccgaaagtg acgatgtatc aaagttaatt caaatgacta tagatagaac tgacgttcat
     3241 caaatcaagt caacagcgta tgattttcta agagtattaa ttagttgtca tattgatagg
     3301 cctctctggg catgaaaaga tggacttatt catgaatttc tagagggagg ccatctggat
     3361 caaagaagaa ggccattttt ttaccatcaa aatcatctgt tcgcaggact tcatgtcgga
     3421 tgtccaaacg gtcaaactct tccagacaag cttccacatc tgcaacctgg aaggctagat
     3481 ggcgtagacc tgtatgttca ggattgggca tggccggacg cattggcgca tctggtttga
     3541 tgaagatttc caagatcaga ttgccttttt tgacattgaa gagaatatca tttttttctg
     3601 ggcgaatatg ctcatccacc tgttcaaagc ctagtttctc aacataaaat tctcttgttt
     3661 tgtcatagtc actaccgata attgcaatat gatgaactgc atcaaatttc attattgtgt
     3721 ttccaatacc tttctaggtt ttgttccttc agctggacca atgacacccg ctgcttctag
     3781 ctcttccatg aggcgtgttg ccctgttaaa accaacagac aagcgtcgtt gaatcataga
     3841 ggcgctggct ttttgtgtct caactaccaa ggcacgtgct tcgttgaaaa gtggatctcc
     3901 tccagtatca ccaaaacctg tatcgccatc accttctgcc acttcaccag gatcaaagga
     3961 ttcatcataa tctgcatctg cttgatcctt gataaagcca actattgctt ctacatcatc
     4021 atcggatata aaggaacctt gcaagcggac tggatggttc tcatcaatcg gtttaaagag
     4081 catgtcaccg cggcctaaga gtttttcagc accgttttca tctagaatcg tccgactgtc
     4141 tgtaccgctt gatacggcaa aggcgatacg agatggtaca ttggctttaa tcaaacctga
     4201 aataacatca acagatggtc gctgggtagc aagaatcatg tggatacctg ctgcacgagc
     4261 cttctgtccc aagcgaataa tggcatcttc cacttccttg ctggctacca tcatcaagtc
     4321 tgccaactca tctacgatta ccacaatgag aggaagtgga atctgttttt cttctgaacg
     4381 gctattaaat tcttcgacct tcgcattata accttccaag tttcggacac caatttggct
     4441 aaacaactca tagcgtttct ccatttcatc cactactttt tgaagagctc gagcagcctt
     4501 gcgtgggttg gtcacaacgg gaatcaatag gtggggaata tcattataga ccgataactc
     4561 gaccattttg gggtcaatca tcatgaactt aacttggtct ggaccagctt tcatgagaat
     4621 tgaagagatg ataccgttga cagcaacaga cttccctgaa ccagttgaac cagccaccaa
     4681 tagatggggc atacgtgcta ggttaaagga acggacggaa ccattgacag ccttaccgag
     4741 aggaatttcc aaaagcttgt ctgggtctgt tttagattgt tcccagagct cacggaaagg
     4801 tactgtagcc acttcggagt ttggaacttc aataccgacc agagattttc ctgggatagg
     4861 agcttcaata cggacatctt tagccgcaag ggctagagcc aaatcatcag ctaggttaga
     4921 aatccgattg acacggacgc cgactgctgg tttgacttca tacttggtta ccgatggtcc
     4981 aatctcagca cgctcaacta ctaccttgat tccaaaactg gcaaaggtgt cttctaaaac
     5041 tttaatattc tgacgaacaa ttcgtttttc attggactga ctcttagctt ttataggagc
     5101 aaataagtct atggacggaa gtttatatgc tagtctctgc ttaggcttga aatcaatctg
     5161 cacatctatc tcgtccttct caaactcatt tggttcagaa gtctggcttg ccgagtttga
     5221 ttgtgctggt tggtcatctt ctgccaacat aataggaaat tcatcttcta tgtctaagtg
     5281 gtcatattct gaaataatcg gtacttgaac aggctcttct tcaaggattt cacccgtttc
     5341 taaatcgaca tgtctatcta ccagtgccaa agcccgagct gactcttcct cttcctctag
     5401 acgaaggcgt tcctcttcta gacgagctaa ttcttgctga cgtttttctt ctcgttcagc
     5461 cttcttctgt gttcgaagct ctgctctttt tacctgcgtc tccaccaact tatctttggc
     5521 aaccgccaag ccatcggcaa catcataaac agaccatggg cttacaaaaa agataccaaa
     5581 tgctatcact agaagaccga tgaagaagga accgacattt gcaaagagga aggagactgg
     5641 cagatagaag atacttccaa tcatccctcc gcctagaaat gctgttacct gaaacttagc
     5701 taggtctgac aaggccaatt ttagagtatg accgaataag tcactccctt gataggtcca
     5761 gtctaagtaa gcctgaaatt caattaataa cccgataaca atcagccaga agcccgatat
     5821 ggtcccttct ctttcgcgta agactttggg aataagaagg tagataaatg cacccgccaa
     5881 taatagatag gccagactac caaataatag ccggaaaata ttgtagctgg tcactccaaa
     5941 cacacctagg cgactggctg caaaagcaat gaataaaagc cccaatactc gaagggtgat
     6001 atttttaaca cgttcttgct gtgctaattc tgccttggtt ggtcgcctcg tagcctttcc
     6061 ttttttactt gtctttctca tagatacctc aactttctca atctctttat tctatcataa
     6121 ttgggaagaa ttttcatgat agaaaaaacc ctgcaaatac agggtttctc ttggagtgaa
     6181 agaataattt aagagggcaa ataatattat agagcgttta gaataatctg accttctacg
     6241 tagaaggttg atgtcaaggt ttcttgacca tcattaataa agatttccaa acagttggtg
     6301 tcaaccaaaa cctctagctc tgttgcttga accgtgaccg ctcgacgaac agttgaccat
     6361 tcctcctcac caacttgctg gattttgaga gagctacggt tgatatagac ttcttgtttg
     6421 gctttatcat agccaaattg aagatagtct tggtcgtttc caagttttag ttccaaactg
     6481 tcttccagtc taagagagag cttgcttggc ccttggatac tttgtccaat ttcaatatcc
     6541 ttgaaggctt caaagacact tgttggcaga acttggtgca atccattttc agtcttcttg
     6601 agaatgcgtg gaagggtcat ttgaccatac cacttatggt ctaggtcatt ggttacaagc
     6661 ggacgtcccc atgtatgcat ccaggcaatc atgacacgtt ccccttctgg accttcagtc
     6721 gtttgagctg catagaaatc atgcccatgg tcgatttcct tgaaggattc tgccacaaat
     6781 accttcttgt cccagtccac atgacccatc acaaagatat tggagttgat attgataaag
     6841 tcattcccgt ctttttggta acgcattgga gagataatca ggtactcttg accgtccact
     6901 tcaaagtagt caggacattc ccagacaaag ccttggttgg cctcaccttt caagaagata
     6961 gactcaaatt tccaatccgt caggttggga gagctgagta agacgataca gccgacattg
     7021 tccttgtgct tggttgcgac aacagagtag tagtgaccat ctttctcaaa aatcttcgga
     7081 tcgcggaagt cattggcaat cacttcttct ggcagtccat ctgctgttgc gactggattc
     7141 tgctcgattt tttcaaagtg aataccgtca gttgagaagg ccatgttctg aacttggcta
     7201 actgtcccgt cttcattgtt gatatgaccg gtatacatga gccagaggac atcatctttg
     7261 acaatggcag agcccgagaa acagccgtct ttatcgtaag ctttatcagg agctagggca
     7321 actggaagct gttcccagtt gaccaagtcc ttacttttcg catgccccca gtgcatcggt
     7381 ccccaaacgc tttcgtaagg gttgaattgg tagaacaaat ggtattctcc acggaagtag
     7441 ataaatccat tggggtcgtt tatccagcct gtttctggtg ttaaatgggc ttgcggtttg
     7501 aaaatcggat tgacgtttcc tttttctgtc tgaataaatt gattggcttc tactactgtt
     7561 ttcacaggca gcctcctttt gattactgat tttctttata ctgatcgtag tatttttgct
     7621 tgatagctaa ccagtcagaa agaccatagc tttccaattc tttcaagtag ctatcccact
     7681 cttcttcgat accgccgttt acaatccatt ctgcacgttt acggtagatg aaatcgttca
     7741 tatctgcttc aacttgggca atcttgtcca aatcttcttg ttccatgaag acacgagggt
     7801 agatgtcatt gttggtcaca tagtcaagat aggttgcgtg catcaagtcc aaacgccatt
     7861 tcgcatcgtc tggcatggtt gttacagtac catagtattc atcaagaata gccagtggtc
     7921 caccaacttc tgtcttctga cgcaattcgc ccggtgcagt accgttcaac ggcaagtgtt
     7981 tcaagctatc tgtagctgca tcatattcga agatgttttg ttgggtttca tcaccatagg
     8041 taccccagtt gttttgaaca gactggattg gtttgtattg ggcatcaatc catttagcag
     8101 tcaattccaa gtttttatta gctgatgtga tgaccatgcg gtcacgttgg aagcccatac
     8161 cgtttgtacg agtgatatgc ttggttccat caggacctgc caatactggc aagatgtcat
     8221 aggtctcatt cataccagtg atgttggcct tgtcccatgt gaagtacaca ccatagaggt
     8281 tttcacttcc ttttgccaag taggtattcc agtcttgctc aaaggcttct gggtcaatga
     8341 ggcctttttc ttgaagggta cgcatgaact caacaccttc tttgtaatct tcattgtccg
     8401 ctgtaaagtc aacggtcccg tcattgttta cgacgatatg gtcatcgttg tcaccgacac
     8461 cgaaggcacc aaagagaatc ttgaagtctt cgccgccgtt accgttgaca aaggacattg
     8521 ggatttcatc cgccttgcca tttccgtttg gatcttgggt cttgaaggct tcaaggacct
     8581 taatcaagtc atccgttgtt tttggcattt caagaccgag tttgttcaac cattctttgt
     8641 tgatccaagc aatatcgtta acagtgtgga ttgattcctt accttcgccc aattcttcaa
     8701 tccatgggaa tgagtagata tggccgtctg gagccgtaat cattgaacgg tattctggat
     8761 tttcatccaa aattttctta aggtttggca tatgcttatc aatcaagtct tcaactggta
     8821 cgataacccc ttgttttgcc cagttcatca attccacatc agaagccccg tcgttatgga
     8881 tggcatctgg caaatcacct gaagcaatgt ccaagttccg cttttcacca aagtctgact
     8941 ggtagtttgt ccaatcgata tgcacacctg tttcttcctc aagacgttgg aaaatcaact
     9001 tatcatttgg gtctgctgga gctagggggg agctagctgt cataaactta agcgtaactg
     9061 atttttcaag cgggaattga acatctttca attcataatc tgggcttgaa gcagtatttt
     9121 ttgagccaca agcagccaat aaaacggtac ttgctagaag cgtaacaact gtttttccaa
     9181 atttgtgttt catcagaaac aacctcctat atttgtaaat tgatttttta gaatatcttt
     9241 cagtttcctt ttattactag gcaacgagct gaaggctgta ctgaagtacg gcatagcgag
     9301 ttcaaataat acatgattat ttgaagctgg agataaggaa acgaagtttc ctctttcgtc
     9361 gacgtaagaa aagagaaaat gaaagatatt tattaacctt ttaatgaacc agccatgatc
     9421 cccttgtcaa agtatttctg gaagaatgga tacatgacaa tgagtggcaa gctagagatg
     9481 acaatggtcg catacttgat catttctgcg attcgtttca tttcattcat ggcagcctgg
     9541 gcaccaatca tgtccttagc aggctcgctt tgaatgagga ttttacgtag cacaagttgg
     9601 agtggctcca agtctgggtc cttgatgtaa atcatggcat caaagtaaga attccattga
     9661 ccgacaaagg catataggaa aagtacaaac atgattggct tagctaatgg aatcatgatt
     9721 ttccagaaaa tctgaaattc attggcaccg tccataaccg ctgcttctac caactcatct
     9781 ggcaagcctt ggaaataggt ccgtgccaag atgatattcc acacattgac tgcaccagga
     9841 ataataatgg cccagacagt attcaacatt cccaaatctt taaccaagag ataggtcgga
     9901 acaagtccgc caccgaagaa catggtgaaa acgagaaagg cattaatcca ttttttcccg
     9961 accaaatctt ttttagagag cgggtaggca gtcatgactg agatagccac ggtcacgaag
    10021 gcaaaggaga aggaataaaa gagggagttg aaaaatccgc gtaggataga cttgtctgaa
    10081 aatacccgtt gataaccttc tactgtccag tcctttggat taaagctaat gcccttgctg
    10141 accaaagcct gcggatccat gaaggaagcg actaggatat agaccatagg cacaactgtt
    10201 atgagaacaa gaaatgccag caagatttta ttgacaatca gcattcgctt gtcaaatttt
    10261 gtgtacattt gtgtattcat acgaacctcc tataagcctt gtccgtcgtt aatgcgtttg
    10321 acaatcttat taactgcaat cagcaggata atattgataa gtgcgttaaa taagccaacc
    10381 gctgttgaat aagagtagtc acctgacacc aaaccgattt tatagacata ggttgaaatg
    10441 atttcagaac tggttaggtt gagggaggtc tgcatcaaga aggctttttc gtaccccacg
    10501 ttcatgatac ctccagctgc taagataaac tgaatgacca taactggttt tagtgctggt
    10561 aagtcaatat gccagattcg ttggaaaata tttgcaccgt ctagcttagc cgcttcaatc
    10621 aaggctggat caacattgac caaggtagcc gtgtaaagag tcgatgccca gcccattacc
    10681 tgccaaatac cactgaggat gtagaggggt ctgaagaagt ctgggtctgt caggaagttg
    10741 gcttcaatgc caaacatacc taggatggaa ttaaccggtc ctcctactga gaagaagagg
    10801 aaaatcatac cgacgatgac aacgactgag ataaagtttg gagcgtagag caccaactga
    10861 atccgttttt tagccttatc gctcaatagt tgattgagca tgatggccaa aataattggt
    10921 ggaagaaatc ctaagagcag accgtagaca ctcaatttca aggtgttggc aagtaaggtg
    10981 ccgaaatttg gagaggaaag aaacttggta aactcttcaa acccaatcca ctcactacca
    11041 agcacccctt ttaaaggatt atagtcttta aaggcaatca gtactccgta cataggaatg
    11101 taacggaaga tgaaagtcaa gaccaagcct ggtagtaaca tcagtaacaa gagtttttgt
    11161 tgcttgattc gatccacgag cccagcttgt tgcagttttg atgttttcat aagaactcct
    11221 ttttgaaacg tttcaaattt attgatttta gaaagaggat gtgaacctct ttttgaaacg
    11281 tttcattttt cttgtaaaaa taaaaccaac tgtttattga tgattttatt ttatcattgt
    11341 aaccgttttc tgtcaactac ttttttaaaa tttttttgta gtttttcctt caagatagga
    11401 aatcggtaga gtgacttgta gggggcgctc tcttttctca ataatatcaa ttagacaggc
    11461 aacggcttcc tgggccatag actccaaagg ttgacgaatg gtcgaaaggg ctaaagattt
    11521 ttcacttgcc aactgaatac catcatagcc aatcacctgc acatcttctg gaacccgtcg
    11581 tcccagtttt tccaaaacag caagtgtgtc tagggccgta aaatcattga tggtgaaaat
    11641 ggcgtcaacc tgtggattgt taatcagaaa ctcttcgact tgaccaacaa agtcatcata
    11701 tggctcttcc aagtccaaaa catgacaagg aaaacctgcc tctaaaatcc gcttttcaaa
    11761 atacaatcta cgcttcttgg tttcgttgat ggttttattg tgcccaccga taaaggcaaa
    11821 atgacgacca ccttttgcaa tcaaggtatt cgctgccagt tccgcaccag ctcggttatc
    11881 cgaactgaca caggctatgt ccttgttttc ataaacacga tcgatactga caaatggaat
    11941 gttggaggac aggtaatctt caatcgggct ataagtgata gcaataatgc cgtccacctt
    12001 attttgttgc agcatagtca gataatccag ttcccttttt ggaccagaaa tattgcaaag
    12061 gagcaacttg taatttttct tactcagttc cacttcaaca tgataggcaa attctccaaa
    12121 aaaaggatgc caaactgtcg gaataatcaa ggcgatggtt tcggttttgt tcttcttcat
    12181 cccacgagca taaacatctg gaatgtaatt taattcccga atggctgtat tgactttttc
    12241 aagcgtactt tccttaatac ctggttcatt gttgataact cttgatactg tcccaacact
    12301 gacccctgca actcgagcta catctttcat agtaattgat ttttgttctt ccatggttta
    12361 cccctttctt ttgaaacg
//
LOCUS       DAZQCJ010000055        12367 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000055 DAZQCJ010000000
VERSION     DAZQCJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12367
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.056"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..218
                     /locus_tag="ACHU66_001356"
     CDS             <1..218
                     /locus_tag="ACHU66_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389636.1"
                     /translation="IVYFKNGNFYQRLQSLLALLTKRHESSHTIQHLTGVDVVLKSKE
                     KLLLQIDGETCTANEVTLTYQERSMYL"
     gene            228..431
                     /locus_tag="ACHU66_001357"
     CDS             228..431
                     /locus_tag="ACHU66_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HFR3389637.1"
                     /translation="MYQLGSFVEMKKPHACVIKSTGKKANKWEVIRLGADIKIRCTNC
                     DHVVMMSRHDFEQKMKQVLPSEA"
     gene            535..900
                     /locus_tag="ACHU66_001358"
     CDS             535..900
                     /locus_tag="ACHU66_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR3389638.1"
                     /translation="MNQLAQQIRILRTAKNLSQDELAEKLYISRQAVSKWENGEATPD
                     IDKLVQLAEIFGVSLDYLVLGKEPEKEIVVEQRGKMNGWEFLSEESKRPVENKEIFLF
                     ILSMAICIFVVWILTMITK"
     gene            1012..2127
                     /gene="ychF"
                     /locus_tag="ACHU66_001359"
     CDS             1012..2127
                     /gene="ychF"
                     /locus_tag="ACHU66_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR3389639.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KDSVAEFAVLEKIKPVLEDGKSARTVDFTDEEQKIVKQLFLLTTKPVLYVANVDEDKV
                     ADPESISYIQKIRDFAATENAEVVVISARAEEEISELDDEDKGEFLEALGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQCAGIIHSDFEKGFIRAVTMSYDD
                     LMTYGSEKAVKEAGRLREEGKEYVVQDGDIMEFRFNV"
     gene            2285..2854
                     /gene="pth"
                     /locus_tag="ACHU66_001360"
     CDS             2285..2854
                     /gene="pth"
                     /locus_tag="ACHU66_001360"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR3389640.1"
                     /translation="MTRLIIGLGNPGDRYFETKHNVGFMLLDKIAKRENVTFNHDKIF
                     QADIGTTFIDGEKIYLVKPTTFMNESGKAVHALMAYYGLDATDILVAYDDLDMAVGKI
                     RFRQKGSAGGHNGIKSIVKHIGTQEFDRIKIGIGRPKGKMSVVNHVLSGFDTEDRIEI
                     DLALDKLDKAVNVYLEEDDFDTVMRKFNG"
     gene            2854..6348
                     /gene="mfd"
                     /locus_tag="ACHU66_001361"
     CDS             2854..6348
                     /gene="mfd"
                     /locus_tag="ACHU66_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR3389641.1"
                     /translation="MNILDLLHKNKQINQWQSGLNKSTRQLLLGLTGTSKSLVMATAY
                     DSMAEKIMIVTATQNDAEKLVADLVAIIGSENVYNFFTDDSPIAEFVFASKERTQSRI
                     DSLNFLTDSTSSGILVASMAACRVLLPSPETYKGSKIKLEVGQEIEVDKLVNNLVNIG
                     YKKVSRVLTQGEFSQRGDILDIFDMQAETPYRIEFFGDEIDGIRIFDVDSQKSLENLD
                     EISISPASDIILSSEDYSRASQYIQTAIEQSTLEEQQSYLREVLADMQTEYRHPDLRK
                     FLSCIYEQSWTLLDYLPKSSPLFLDDFHKISDKQAQFEKEIADLLTDDLQKGKTVSSL
                     KYFASTYAELRKYKPATFFSSFQKGLGNVKFDALYQFTQHPMQEFFHQIPLLKDELTR
                     YAKSNNTVVIQASSDVSLQTLQKTLQEYDIHLPVHAADKLVEGQQQVTIGQLASGFHL
                     MDEKLVFITEKEIFNKKMKRKTRRTNISNAERIKDYSELAVGDYVVHHVHGIGQYLGI
                     ETIEISGIHRDYLTVQYQNFDRISIPVEQIDLLSKYLASDGKAPKVNKLNDGRFQRTK
                     QKVQKQVEDIADDLIKLYAERSQLKGFAFSPDDENQIEFDNYFTHVETDDQLRSIDEI
                     KQDMEKDSPMDRLLVGDVGFGKTEVAMRAAFKAVNDGKQVAILVPTTVLAQQHYANFQ
                     ERFAEFPVNVDVMSRFKTKAEQEKTLEKLKKGQVDILIGTHRLLSKDVVFADLGLLVI
                     DEEQRFGVKHKERLKELKKKIDVLTLTATPIPRTLQMSMLGIRDLSVIETPPTNRYPV
                     QTYVMETNPSVIRDAMLREIDRGGQVYYLYNKVDTIEQKVSELKELVPEATIGYVHGQ
                     MSEIQLENTLYAFVEGEYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSSRIAYAYLMYRPDKSLTEVAEKRLEAIKGFTELGSGFKIAMQDLSIRGAGNILG
                     AAQSGFIDSVGYEMYSQLLEQAILEKQGKATQRQKSNSEVNLQIDAYLPSDYIGDQRQ
                     KIEIYKRIKNIDSRVNYQELQEELIDRFGEYPDVVAYLLEIGLLKSFLDQVFCHTVLR
                     RQHQVTVTFEPMACQIFLTQDYFEALSVTNLKAQITENKGKLAVVFNIQQKKEYEILE
                     ELISFAEKLKEIKARKAE"
     gene            6398..6670
                     /locus_tag="ACHU66_001362"
     CDS             6398..6670
                     /locus_tag="ACHU66_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFR3389642.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIQFGNKLLTVKVLEMKDSTKKEDALKMYEIISEKRIEKDEEI"
     gene            6657..7025
                     /locus_tag="ACHU66_001363"
     CDS             6657..7025
                     /locus_tag="ACHU66_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFR3389643.1"
                     /translation="MKKSKILQLNNAFIQSERKKTQHQLAERQQKNRFMGAILILVIF
                     LFMLPAYNLVGTYTNIQQQEKKLAELEKNYEELTKEQKQEAEMVAKLKNEEYAAKYVR
                     AKYQYSKEGEFVYNIPGLPK"
     gene            7022..7135
                     /locus_tag="ACHU66_001364"
     CDS             7022..7135
                     /locus_tag="ACHU66_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0009 family protein"
                     /protein_id="HFR3389644.1"
                     /translation="MTESIFKIVEQFLQHSDEKLEELNQKNRELKLEENES"
     gene            7148..8428
                     /locus_tag="ACHU66_001365"
     CDS             7148..8428
                     /locus_tag="ACHU66_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFR3389645.1"
                     /translation="MQKKLLVWLLPVLFGWQVVDSTEIPFELTAQEEYELTHTIYDQY
                     FQTIPQNPNVFQTENLYSDEELTIAGGQLQPNQHFSITDVLVNSKKELVFQIDDKGYI
                     LASRHLLFDDVIVTETTVEQTYWTKKGFTLLTSPIANEATEIKNDLQPYQAVMVSKIV
                     TTSLGDFAYVTDKGWIAVNNLSKTDNRVEAVQELLTNKYSKDTIGIYVKQLSTGQTAG
                     VNQEKLFYSASIAKLPILYYVQEQLNAGYIDLTTKVKYTAESISFPGAYVAGGSGSLS
                     KTPDNKDYSVEELINKTAKESDNVASNLLSYYVANKFDSNFYQVITAKTGSEWSMVTR
                     ETSAEIAGKMMESLYIQNGYVLESLLSTQFDNQRISKDISVPVAHKIGDADDVKHDVA
                     IVYAGSPFVLSIFTDKSNYDEITQIANDIYRILK"
     gene            8429..9697
                     /gene="tilS"
                     /locus_tag="ACHU66_001366"
     CDS             8429..9697
                     /gene="tilS"
                     /locus_tag="ACHU66_001366"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR3389646.1"
                     /translation="MKDRFLKVTLDGHFFDKHNKVLVAVSGGLDSMNLFHLLYECKQV
                     LGIELGIAHINHGQREESVIEEKYLRQLAEESNVPFYLSYFEGVFSEEAARKWRYGFF
                     ATIMEKEGYTALVTAHHADDQAETVFMRLIRGGRLRHLSAIQPTQPFATGELIRPLLS
                     FKKADFEKLFHFEDCSNADFKYFRNRVRNDYLPKLKQENPKIEFTLNNFAVDTNNLFQ
                     ALRDLTQDLSVTDVMSFQQQTPAVQSYLLEEYLEKFPDLQLSRPQFNEVLHILRSKAN
                     YYHLLKNDYMLEKDYHRFQIYKIGPETDSQPEKIMIKSEGIFSYGSYIFSLNHPLEEA
                     DSILYFPTENPIVVRRRQAGDTILINGVNKKLRRWFIDNKISQKVRQNALIIEQNGEI
                     YGITNLVSSDLSKSVKNDIIKATLYIKMKE"
     gene            9705..10247
                     /gene="hpt"
                     /locus_tag="ACHU66_001367"
     CDS             9705..10247
                     /gene="hpt"
                     /locus_tag="ACHU66_001367"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR3389647.1"
                     /translation="MLDKDIKKILVSEEEIVAKCKELGQILATDYADKNPILVGILKG
                     SIPFMAELMKHIDAHVETDYMVVSSYHGGTESCGTVKIIKDLDNSVAGRHIIFIEDII
                     DTGRTLKELKELFALRQAGSIKIATLLDKPEGRVVEIEPDYTCFTIPNEFVVGFGLDY
                     DENYRNLPYVGVLKEEVYTK"
     gene            10269..12239
                     /gene="ftsH"
                     /locus_tag="ACHU66_001368"
     CDS             10269..12239
                     /gene="ftsH"
                     /locus_tag="ACHU66_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR3389648.1"
                     /translation="MNNQPNKGFIKNPFLVILVIATIVTAFQFFNAGQQVATQEISYS
                     QVVTELKNNNVSEITYQPNSSVIEITGKYKTEQEAKDELASSIKLFQVSSKVKYKNFK
                     SLILPSETNLSELQALANENGVKVTIKPESSNGLWLNIVFNLLPLIIAGVFFMMMMNQ
                     GGGARGAMNFGRNKAKALEQSNIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASCVRSLFEDA
                     KKAAPAIIFIDEIDAVGRQRGVGMGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATN
                     RSDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAADVDLKLVAQQTPGF
                     VGADLENVLNEAALVAARRNKTVIDASDIDEAEDRVIAGPSKKDRQVSAKEREIVAYH
                     EAGHTIVGLVLSNAREVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGG
                     RVAEEIIFNTQTTGASNDFEQATQMARAMVAEYGMSEKMGPMQYEGSHAMFGGQTTQK
                     HISEQTAYELDNEVRDLLNEARNKAADIIQSNRDTHKLIAEALLKYETLDSVQIKSLY
                     ETGKMPENIERDDEDVHPLSYEEVKEKINTKE"
BASE COUNT         4158 a         2082 c         2539 g         3588 t
ORIGIN      
        1 atattgttta cttcaaaaat ggaaatttct accaacgtct acaatcattg ttagccttat
       61 taacgaagag gcatgaatct tctcatacaa ttcagcattt aacaggggta gatgtagttt
      121 taaaatcaaa agaaaaatta ttattgcaaa tagatggaga gacatgcact gcaaatgagg
      181 taacgttaac ctatcaggaa agaagtatgt atctttaagg aggaagtatg tatcaattag
      241 gatcatttgt cgaaatgaaa aagcctcatg cctgtgtcat caaatcgacc ggaaagaagg
      301 caaataagtg ggaggttatc cgtctaggag cggatattaa aatccgttgt accaactgtg
      361 accatgtagt catgatgagc cggcatgatt ttgaacaaaa aatgaaacaa gttctaccaa
      421 gtgaagccta gttgacagtt tgcaactgct ggttgcgaat atttcctaaa aagtgctagt
      481 caactgttcc tttctaaact ataatagact tagaaaaaag aggaggaaat gatcatgaat
      541 cagttagcac agcaaattcg aattttacgc acagccaaga acctatccca agatgagttg
      601 gcagagaaac tctatatttc ccgtcaggct gtttccaagt gggaaaatgg cgaagcaacg
      661 ccagatattg acaaactggt tcagctagca gaaatctttg gtgtcagtct ggattatctg
      721 gttttaggaa aagaacctga gaaggaaatt gtggtggaac aacgaggaaa aatgaatggt
      781 tgggaatttt taagtgaaga atccaagcga ccagttgaaa ataaagaaat ttttctgttt
      841 atcctctcta tggccatctg tatttttgta gtttggatct tgaccatgat tacaaaataa
      901 tggattattt attcagcatt atttttaacg aagcttgcta atcggcaagc tttttctcta
      961 cttttctgct ataatagtca tgattgaatt tttaattgga gagtaaaaaa catggcttta
     1021 acagcaggaa tcgtcggttt gccaaacgtt ggtaaatcaa ctctatttaa cgcaattacc
     1081 aaggcaggag cagaagctgc aaactaccct ttcgcgacca ttgatccaaa cgtcggcatg
     1141 gtggaagtgc ccgatgagcg tttgcagaaa ttgacagaac tcatcacccc taaaaagaca
     1201 gtgccaacga cttttgaatt tactgatatt gccggtatcg taaaaggtgc ttctaaaggt
     1261 gaaggacttg gaaataaatt cttggccaat atccgcgaag ttgatgcgat tgtccacgtt
     1321 gtgcgtgcct ttgacgatga aaatgttatg cgtgaacaag gccgtgaaga cgcctttgtt
     1381 gatcctatcg cagatattga tactattaac ctagaattga ttttggcgga cctagagagc
     1441 atcaacaagc gttatgcgcg tgtagaaaaa atggcccgta cgcaaaaaga caaggattct
     1501 gtcgcagaat ttgcagttct tgaaaaaatc aaacctgtcc tagaagacgg aaaatctgct
     1561 cgtacagttg acttcacaga tgaagaacaa aaaatcgtta agcaactctt cctcttgacc
     1621 actaaaccag tcctctatgt tgccaacgtc gatgaagaca aggtagcaga tcctgagtct
     1681 atcagttata ttcagaaaat ccgtgacttt gcggcaacag aaaatgcaga agtggtggtg
     1741 atttctgcgc gtgctgaaga agaaatttca gaactcgacg atgaagacaa gggcgagttt
     1801 ttagaagcac tcggattaac agaatctggc gtggacaaat taacacgtgc agcctatcac
     1861 ttgcttggtc taggcaccta ctttacagca ggggagaagg aggttcgtgc ttggaccttc
     1921 aaacgtggta tgaaagctcc tcagtgtgca ggcattatcc attcagactt cgaaaaaggt
     1981 tttatccgtg ccgttaccat gtcatacgat gatttaatga cctacggctc tgagaaggct
     2041 gttaaagaag caggacgcct tcgtgaagaa ggaaaagagt acgtggttca agatggggac
     2101 atcatggagt tccgctttaa tgtttgattg ctaaactgtc cttattcttc gttgttttac
     2161 cttgccatac catcagtatt gtctgcggtt tcaacgccta gactaagaac agtttatcgc
     2221 atcaaagagg atgattactt ggttggaaag ctttttccag ccttttggta tttaaggaga
     2281 aaaaatgaca cgattgatca tcggtctggg aaatcccgga gaccgctatt ttgaaacaaa
     2341 acataacgtt ggttttatgc tgctggataa gattgctaag cgtgaaaatg tgacctttaa
     2401 tcacgataag attttccaag ctgacattgg cacaaccttt attgacggcg aaaaaatcta
     2461 tctagtaaag ccaacgacct tcatgaacga atcaggtaag gctgttcatg ccctgatggc
     2521 ttattatggt ctggatgcaa ctgatatttt agtagcttat gatgacttag acatggctgt
     2581 aggaaaaatt cgtttccgtc aaaaaggctc agctggtggc cacaatggta tcaaatccat
     2641 tgtcaagcat attggaacac aggaatttga ccgtatcaag attggtattg ggcgtcctaa
     2701 aggaaaaatg agcgttgtca accacgtctt atcaggattt gatacagaag accgtattga
     2761 aatcgactta gcactagata aacttgacaa ggctgtcaac gtatatctcg aagaagatga
     2821 ttttgataca gtaatgagaa agtttaacgg ataatgaata tactcgattt acttcacaag
     2881 aacaagcaaa tcaatcagtg gcagtctggc ttaaacaagt caactcgcca actattatta
     2941 ggactcactg gaaccagtaa gtcattagtc atggcgactg cctatgatag tatggctgag
     3001 aaaataatga ttgtgactgc tactcaaaat gatgctgaga aattagttgc agatttagta
     3061 gccattattg gaagtgagaa tgtttacaac ttttttacag atgatagtcc tattgccgag
     3121 tttgtttttg catcaaaaga acggactcaa tcaaggattg acagtttaaa tttcttgaca
     3181 gattctacta gttcaggaat tttagttgct agtatggctg cttgtcgggt cttattgcca
     3241 agtcctgaga cttataaagg ttctaaaata aagcttgaag ttggtcaaga aatagaagtt
     3301 gacaaacttg taaataatct tgtcaacatc ggctacaaaa aagtgtctcg tgtattaacc
     3361 cagggagaat ttagccaacg aggtgatatt ctagatattt ttgacatgca agcagaaact
     3421 ccttaccgaa tagaattttt tggagatgag attgatggca ttcgtatttt tgatgttgac
     3481 agtcaaaaat ccttagaaaa tctagacgaa atctcgatct ctccagcttc cgatatcatt
     3541 ttgtcttcgg aagactatag tagagctagc caatacattc agacagctat tgaacaatcg
     3601 accctagagg agcaacagtc ttacctacga gaagtcttag ctgatatgca gacggagtac
     3661 cggcatccag atttgcgtaa attcttgtcc tgtatctatg aacaatcatg gacattgttg
     3721 gattacttgc caaagagttc tcccttattt ttggatgatt ttcataaaat ctctgataag
     3781 caggctcaat ttgaaaaaga aattgcagac ttgttgacag atgatttaca gaagggtaaa
     3841 acagtgtcaa gtttgaaata ttttgcctca acttacgctg aattgagaaa atacaaacct
     3901 gccaccttct tttcaagttt tcaaaaaggt ttaggcaatg taaagtttga tgctctttat
     3961 caatttacac agcatcctat gcaggaattt ttccaccaga ttccactgtt aaaagatgaa
     4021 ttgactcggt atgcgaaatc aaacaatacc gttgttattc aagcgagttc tgatgtgagt
     4081 ttacagactt tacagaaaac tttacaagag tatgatattc atcttccagt acatgcagca
     4141 gataagttag tagaaggtca gcaacaggtt acgattggtc agttagcttc aggttttcat
     4201 ttgatggatg agaaactagt tttcatcact gaaaaagaga ttttcaataa gaaaatgaag
     4261 cgtaagacac gtagaaccaa tatttctaac gctgaacgta ttaaggacta tagtgaatta
     4321 gcagttggtg actatgttgt tcaccatgtt catggtatcg gtcaatacct aggaattgag
     4381 actattgaaa tttcaggcat tcatcgtgat tatttaacgg ttcaatatca aaacttcgat
     4441 cgcatttcta ttcctgtaga acaaattgat cttctctcca aatacttagc gtccgatggg
     4501 aaagctccaa aagttaataa actaaacgat ggccggttcc aacgaactaa gcaaaaagtt
     4561 cagaagcaag tggaggatat tgcagatgat ttgattaagc tttatgcgga gcggagtcag
     4621 ctgaaaggct ttgccttttc accagatgat gagaatcaga ttgagtttga caactacttt
     4681 acacatgtgg aaaccgacga ccaacttcgt tccattgatg aaatcaaaca agatatggaa
     4741 aaagactctc caatggatcg tttgctggta ggagatgtcg gctttggtaa gacagaggta
     4801 gctatgcgcg ctgctttcaa agcagtcaat gatggcaagc aagtagctat tcttgttcct
     4861 acgactgttt tagcccagca acactatgct aatttccagg aacgatttgc ggaatttcct
     4921 gtcaatgtgg atgttatgag tcgttttaaa acaaaggcag agcaggaaaa aacacttgag
     4981 aagttgaaaa aaggccaagt tgacatcttg attggtaccc atcgccttct atcaaaagat
     5041 gttgtttttg ctgatttagg tttactggtt attgacgaag agcaacggtt tggtgtcaag
     5101 cataaggaac gcttgaagga attgaaaaag aaaatagatg tcctaacctt gactgcaact
     5161 ccgattcctc ggacattgca aatgtccatg ctgggaatcc gcgatttatc agtaattgag
     5221 actccaccga caaatcgcta tcctgtacaa acttatgtga tggaaacaaa tccttccgtt
     5281 atccgagatg caatgcttcg tgagatagat cgcggaggtc aggtttacta tctttacaat
     5341 aaagttgaca ccattgaaca gaaagtgtct gaattaaaag aattggttcc agaagctact
     5401 ataggatacg tccatggaca aatgtcggag atccaattag aaaatactct ctatgctttt
     5461 gtagagggtg aatatgatat tctagtgacc acaacaatca tcgaaacagg tgttgatatt
     5521 ccaaatgcta atacactgtt tattgaaaat gcagatcata tgggactgtc aactctttat
     5581 caacttagag gacgtgttgg ccgttccagt cggattgcct atgcctatct catgtaccgt
     5641 ccagacaagt ccttgacaga agtagctgaa aaacgtttgg aggcaatcaa gggctttaca
     5701 gaactaggat caggtttcaa gattgccatg caagatttgt ccattcgtgg tgcgggaaat
     5761 attttaggtg ctgctcagtc aggttttatt gattctgtgg gatatgagat gtattcacaa
     5821 ttgctggaac aagccatctt ggaaaaacaa ggtaaggcaa ctcaacgtca gaagagtaat
     5881 tcagaggtca atttacagat tgatgcctat ctaccaagtg attatattgg tgaccagcga
     5941 caaaaaattg aaatttacaa gcgtataaag aatattgaca gtcgtgtcaa ctatcaagaa
     6001 ttacaggaag aattgattga ccgttttgga gagtatcctg atgtagtagc ctacttgctt
     6061 gaaatcggtc tattgaagtc atttttagac caagttttct gtcatacagt tcttagacga
     6121 caacatcaag tgacagtaac attcgaacct atggcttgtc aaattttcct aacgcaagat
     6181 tactttgaag ctttatctgt gacaaatttg aaagctcaga taacggaaaa taaaggaaaa
     6241 ctagctgttg tgtttaacat tcaacagaaa aaagaatatg aaatattaga ggaattgatt
     6301 tcttttgccg aaaaattaaa agaaattaaa gcaaggaagg cagaataaat ttctcaaata
     6361 ccttgaaaaa tggtaaaata atgaactgag gtaaagtatg agactagata aatatttgaa
     6421 agtatcccgc attattaagc gtcgaacagt tgcgaaggaa gttgccgata aaggacgaat
     6481 aaaagtcaat gggattttgg cgaaatcttc aacagattta aaagtaaatg atcaggttga
     6541 aatccaattt ggtaataaac tactcactgt aaaagtactt gaaatgaaag attcgactaa
     6601 aaaagaagac gcactgaaaa tgtatgaaat tatcagtgaa aaaaggatag aaaaagatga
     6661 agaaatctaa aattctacaa ctgaataatg cctttattca gtcggagcgt aaaaaaactc
     6721 aacatcaatt ggcagaacgc caacaaaaaa atcgttttat gggtgcaatt ctcattctag
     6781 ttatctttct ttttatgttg cctgcctata atcttgttgg gacttacact aatattcagc
     6841 agcaggaaaa gaaacttgct gaattggaaa aaaattatga agaactgacc aaagaacaga
     6901 agcaggaagc agagatggtt gcaaaattga aaaatgagga atacgcagca aagtatgttc
     6961 gggctaagta tcaatactct aaagaagggg aatttgtcta caatattcca gggttaccaa
     7021 aatgaccgaa tctattttta aaattgtaga gcaattttta cagcattctg atgaaaaatt
     7081 agaagagttg aatcaaaaga atagagaatt aaagttagaa gaaaatgaat cgtagaaagg
     7141 agggagtatg cagaaaaaac tgctcgtatg gttattgcca gttttatttg gatggcaggt
     7201 agtcgatagt acagaaatac catttgaact cacagcgcaa gaagaatacg aattgactca
     7261 tactatctat gatcagtatt ttcaaactat ccctcaaaac ccaaatgttt ttcaaactga
     7321 gaatctttat tcggatgaag aactgactat agcaggtgga caattacagc cgaaccaaca
     7381 tttttctatt acggatgttc tagtcaatag caaaaaagaa ttggttttcc aaatagatga
     7441 caagggttac attcttgcaa gtaggcatct actatttgat gatgtaattg ttacagaaac
     7501 tactgttgag caaacttatt ggacaaaaaa aggtttcaca ttgttaacct caccaattgc
     7561 taacgaagct acggaaataa aaaatgattt acaaccctat caagctgtta tggtttctaa
     7621 aattgtgaca acttccctag gtgattttgc ctatgttaca gataaaggtt ggattgcagt
     7681 aaataatctt tctaaaacgg ataatagagt agaagccgtt caagagttgt tgactaacaa
     7741 atattccaaa gatactattg gtatatatgt gaaacaattg tcaacaggtc aaactgcagg
     7801 tgtaaatcaa gagaaactat tctattctgc cagcattgct aaactaccaa ttctttatta
     7861 cgttcaagag caactgaatg ctgggtatat tgatttgact actaaagtga aatatacagc
     7921 cgaatctata tccttcccag gagcttatgt tgctggagga agtggttcac tgtcaaaaac
     7981 tcctgataac aaagattatt cagtggaaga attgatcaat aagactgcta aagagtcaga
     8041 taatgtagct agtaatttac tttcttatta tgtagctaat aaattcgatt ccaattttta
     8101 tcaagtaata actgctaaaa caggtagtga gtggagtatg gttacacgtg aaacttcagc
     8161 tgaaatcgca gggaaaatga tggaatcctt atatattcaa aatggctatg tgctagaaag
     8221 tttgttatct actcaatttg ataatcaacg tatttcgaaa gacatctcag ttccagttgc
     8281 tcataaaatt ggcgatgcag atgatgtcaa acacgatgta gcgattgttt acgcagggtc
     8341 tccttttgtt ttatctattt ttacagataa atcaaattat gatgagatta cacaaattgc
     8401 aaatgatatt tacaggattt taaagtaaat gaaagataga tttttaaaag tgacactgga
     8461 tggtcacttt tttgataaac ataataaagt actagtagcc gtatctggtg ggttagattc
     8521 tatgaaccta ttccatcttt tatacgaatg caaacaagtg ttagggattg aacttggaat
     8581 tgcccatatt aatcatggac agagagaaga atctgtcatt gaggaaaaat atcttagaca
     8641 gttagcagaa gaaagtaatg ttccctttta tctgtcctat tttgaaggtg ttttttcaga
     8701 agaagcagcg cgaaaatggc gttatggatt ttttgctaca atcatggaga aggaaggcta
     8761 cactgcacta gtgacagccc accatgcaga tgatcaagca gaaactgtct ttatgcgatt
     8821 gattagagga gggcgtttac gtcacttatc agcaattcaa ccgactcagc cttttgcaac
     8881 aggagaactt attcgaccac ttttatcttt taaaaaggca gattttgaaa agctatttca
     8941 ttttgaagat tgcagcaatg cagatttcaa atattttcga aatagagtca gaaatgacta
     9001 tttaccaaag ttaaaacagg aaaatcctaa aatcgaattt actctcaata attttgcagt
     9061 tgatacaaat aatctatttc aagcgctaag agatttgaca caggatttat ccgttacaga
     9121 tgtgatgagt tttcaacaac agacacctgc ggtacaaagt tatctattag aggaatattt
     9181 ggaaaaattt cctgatttac agctctctcg tccacaattt aatgaagtat tacacatctt
     9241 gcgttcaaaa gctaactact atcatttatt gaaaaatgat tacatgttag agaaggatta
     9301 tcatcgtttt caaatttata aaataggacc agagacggat agtcaaccag aaaaaatcat
     9361 gataaaatca gagggtattt tttcttacgg ttcatatatt ttttctctca atcatccttt
     9421 ggaagaagcg gatagtattt tatattttcc tacagaaaat ccaattgtag tgaggagaag
     9481 gcaggctggg gataccattt taataaacgg agtaaataaa aaactccgcc gttggtttat
     9541 tgataataaa atctcacaga aagttcgaca aaatgcgctt attatcgagc agaatggaga
     9601 aatttatgga attacgaatc ttgttagcag tgatttgagt aaatcagtaa aaaatgatat
     9661 aatcaaagct accttatata taaaaatgaa agagtagaac aactatgttg gacaaagata
     9721 ttaagaaaat tcttgtttca gaagaagaaa tcgttgcaaa atgcaaagaa ctcggtcaaa
     9781 ttctagcaac agactatgca gataagaatc ctattcttgt aggaatttta aaaggttcta
     9841 tcccatttat ggcagaatta atgaaacata ttgatgccca tgtagaaacg gattatatgg
     9901 ttgtttctag ctaccatggt ggtactgaga gttgtggtac cgttaagatt atcaaggatt
     9961 tggataatag tgttgcaggt cgacatatca tctttataga ggatattatt gacacaggcc
    10021 gcacattaaa agaattgaaa gaactctttg ctttgcgtca agcaggttct attaaaattg
    10081 ctactcttct tgataaacca gaaggtcgtg ttgttgaaat cgagccagac tatacttgct
    10141 ttactattcc aaatgaattt gtagttggat ttggcttaga ttatgatgaa aattatcgta
    10201 atcttcctta tgttggtgta ctcaaagagg aagtttacac aaaatagaaa aaggtttcct
    10261 actatattat gaacaatcaa ccaaataaag gatttataaa aaatcctttt ctcgttattc
    10321 tcgttattgc cactattgtt acagcctttc aattttttaa cgctggtcag caagtagcaa
    10381 ctcaagaaat cagttattca caagtagtaa ctgaattgaa aaataataat gtatctgaaa
    10441 ttacttatca accaaattca agtgttattg aaattactgg taagtacaaa acagaacagg
    10501 aagctaaaga tgagttagct tcatctatta agttattcca agtttctagt aaagttaagt
    10561 ataaaaactt taagtcacta attttaccgt cagaaacgaa tttgtcagag ctacaagctc
    10621 ttgcaaatga aaatggagta aaggttacta ttaaacctga aagttcaaat ggactttggt
    10681 taaatatcgt ctttaatttg ttaccgctta tcattgctgg tgtattcttc atgatgatga
    10741 tgaaccaggg tggtggagca cgtggtgcaa tgaactttgg acggaataaa gctaaagctt
    10801 tggaacaaag taacatcaag gtacgtttct cagatgtagc aggtgcagag gaagaaaaac
    10861 aagaattagt tgaagttgtt gaatttttga aggatccaaa acgctttact aaacttggag
    10921 cacgtattcc agcaggtgta ctactagagg gtcctccagg aaccggtaaa accttgcttg
    10981 ccaaagcagt agcgggtgaa gcgggtgtac cattcttctc catttctggt tcagattttg
    11041 ttgaaatgtt tgttggtgtc ggtgccagct gtgttcgttc attatttgaa gatgcaaaaa
    11101 aagcagcacc agccattatt ttcatcgatg aaatcgatgc tgtcggtcgc caacgtggtg
    11161 tcggcatggg tggcggtaac gatgaacgtg aacaaaccct caaccaactc ttaattgaaa
    11221 tggatggttt tgaagggaat gaagggatta ttgtcattgc cgcaaccaac cgtagtgatg
    11281 ttttagaccc tgcccttctt cgtccaggtc gttttgaccg taaagtattg gttggtcgtc
    11341 cggatgttaa aggacgtgaa gcaatcctta aagttcatgc gaagaataaa ccactagcag
    11401 cagatgtaga tttgaaatta gtagcacaac aaacaccagg atttgttggt gcagatttag
    11461 agaatgttct taacgaagca gcccttgtag ctgcccgtcg aaacaagact gtcattgatg
    11521 cttcagatat tgacgaagca gaagatcgtg tcattgcagg tccttctaag aaagaccgtc
    11581 aagtttcagc caaagaacgc gaaatcgttg cctaccatga agcaggtcat acaattgttg
    11641 gtctcgtact atcaaatgca cgtgaagttc acaaagtaac aattgttccc cgcggtcgcg
    11701 caggtggata catgattgcc cttcctaaag aagatcaaat gcttctttct aaagaagata
    11761 tgaaggagca attggctggg cttatgggtg gtcgtgttgc ggaagaaatt atttttaata
    11821 cacagaccac aggtgcctca aatgactttg aacaagctac acagatggct cgtgctatgg
    11881 ttgcagaata tggtatgagt gaaaaaatgg gaccaatgca gtatgaaggc agtcatgcaa
    11941 tgtttggtgg acaaacaact caaaaacata tttctgagca aactgcatat gagttagata
    12001 atgaagtacg tgatttgtta aatgaagctc gaaataaagc tgcagatatt attcaatcaa
    12061 accgtgatac acacaaactg attgcagaag cattgcttaa atacgaaacc ctagacagtg
    12121 ttcagatcaa atctctctat gaaacaggca aaatgccaga gaatattgaa cgtgatgatg
    12181 aagatgttca ccccctttct tatgaagagg taaaagagaa aattaatacg aaagagtagt
    12241 ataaaaatac tcttggcaag acattccctc actctcaacc actaaaataa ccacaaatcc
    12301 ttcgctaagt acataatctt ggcgagggct ttttttacaa aaaagggcat ttacaagaaa
    12361 aacgaat
//
LOCUS       DAZQCJ010000056        12304 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000056 DAZQCJ010000000
VERSION     DAZQCJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12304
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.057"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            34..1056
                     /locus_tag="ACHU66_001369"
     CDS             34..1056
                     /locus_tag="ACHU66_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR3389649.1"
                     /translation="MLEHSIDIQLKHPDDSFSLFGSNERHLRLMEQELGVVIHARTEK
                     VQVIGQEEHVEQARLVIQSLLVLVSRGLVINTPDVVTAISMAKNDEIEKFVALYEEEI
                     IKDNYGKPIRVKTLGQKLYVDSVKRHDIVFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IVLTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKEQTTRLMEREIIEIAP
                     LAYMRGRTLEDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDISQIDLPRKVKSG
                     LIDATEKLSKIPQIDFVHFSAKDVVRHPVVAQIINAYEQEALAADGRASFETIGEPSR
                     KYNGEE"
     gene            1171..1354
                     /locus_tag="ACHU66_001370"
                     /pseudo
     CDS             1171..1354
                     /locus_tag="ACHU66_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            complement(1725..2873)
                     /locus_tag="ACHU66_001371"
                     /pseudo
     CDS             complement(1725..2873)
                     /locus_tag="ACHU66_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027267.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
     gene            3076..4242
                     /locus_tag="ACHU66_001372"
     CDS             3076..4242
                     /locus_tag="ACHU66_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR3389650.1"
                     /translation="MKKQSPFIIAGIVMLGVVMRAPFTALPAILIDVAAGLRVEVSSL
                     GILTSIPLIMFALCSSLAPRLAAKFGMEKLMALVLLVMVLGSGMRVLNLPALYIGTML
                     VGATIAFINVLLPSLVAANFPKKIGLYTTIYITLMGVAATVASMIAVPIVSSSSWEFF
                     ILLITGLVFMAFLIWLPNVRNNHRFASENQGNQKSSIWKNKAAIAFLIFGGLQSVLYY
                     TEITWLPTISQSVGFSKAEAGLMAGFFNMTAIPMSMIIPAVLSRQTKEMRRNIMLAIS
                     SVTLLGLVMMVLIPTNLILWSALHIILSFSNAALFPYMMLSFTLKTSNSQATAQLSGM
                     VQTGGYLIAAFGPGLLGYSYPIFGNWMPLILALAIVTLAMMWTIVLIEKEDIIL"
     gene            complement(4341..4511)
                     /locus_tag="ACHU66_001373"
     CDS             complement(4341..4511)
                     /locus_tag="ACHU66_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFR3389651.1"
                     /translation="MAKGFGKGFLTGVLSTVALAAGAVFTVHKTIIEPEEKKEAFIEE
                     NRKKAARRRVAH"
     gene            4580..5464
                     /gene="miaA"
                     /locus_tag="ACHU66_001374"
     CDS             4580..5464
                     /gene="miaA"
                     /locus_tag="ACHU66_001374"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR3389652.1"
                     /translation="MKTKVIVVIGPTAVGKTALGIDLAQRYNGEIISGDSQQVYRKLD
                     IGTAKASPEEQAAAVHHLIDVRDVTEGYSAYEFVAEAKALIADITSRGKLPIIVGGTG
                     LYIQSLLEGYHLGGQVDQVQVLAYRAELDSLSDEDLETMAEQAGLMVEGNSRRRIIRG
                     LELKKFGENLENTESGYEPLYICLTDDRQVLYDRINQRVDKMMAAGLLDEVSWLYQEH
                     PQAQAAMGIGYKEFFPYLAGAISLEEAIDKVKQNSRRFAKRQLTWFRNRMQVPFYSVG
                     EPDCKSQIFTAVEEFLND"
     gene            5457..6707
                     /gene="hflX"
                     /locus_tag="ACHU66_001375"
     CDS             5457..6707
                     /gene="hflX"
                     /locus_tag="ACHU66_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFR3389653.1"
                     /translation="MIETQKEQERALLVGVELQQTDNFDMSMEELASLAKTAGALVKG
                     VYTQKREKYDSKTFVGSGKLDEIAQMVEADEIDTVIVNNRLTPRQNVNLEEILGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRNQIHDIERQLKTVEKNRATVRERRLQSGVFKIGLIGYTNAGKSTIMNVM
                     TDKRQYEADELFATLDATTKQINLADKFNVTLTDTVGFIQDLPTELISAFKSTLEESM
                     NVDLLLHIIDASDPNHSEQEQVVLDILKDLDMLDIPRLALYNKLDKTDDSFTPSQFPH
                     VMLSAKDENAKGHIQMMVLAKIKTMFERFEVRVPLAESYKLHDLATLALIENRTYEED
                     VEVVSGYIASANKWKLEEFYDGLS"
     gene            6691..7320
                     /locus_tag="ACHU66_001376"
     CDS             6691..7320
                     /locus_tag="ACHU66_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR3389654.1"
                     /translation="MTDYLDLAMKYGGFTSLDKVYLANKLAGLTDQQKLDFITPPPSV
                     INAYFAEIYQKQGAEEATDYYLTLSQQLCLFPKEPSFAESKPFVRLNLSGKSFGFTYV
                     SADGLAQVFSEGVEEVTESLLFEIAQVFPHYVVFVEEGKIFMKVNPFEDAELEDVETD
                     YLLTQVATTEGLVKISGFNQEEVLEVADGYAGQYYYVWSGRSAILYIKH"
     gene            7331..8260
                     /gene="rnz"
                     /locus_tag="ACHU66_001377"
     CDS             7331..8260
                     /gene="rnz"
                     /locus_tag="ACHU66_001377"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFR3389655.1"
                     /translation="MQIQFLGTGAGQPSKARNVSSLALKLLDEINQVWLFDCGEGTQN
                     QILETTIRPRKVAKIFITHLHGDHIFGLPGFLSSRSFQSSDEQTDIDIYGPVGIRSFV
                     LASLKVSGTRLPYRIHFHEFDVDTVGQVLETDKFTVFAEKLDHTIPCVGYRVIQKDLE
                     GTLDAEALRAAGVPFGPLFGKIKNGQNVTLEDGTEIIASDYISPPRPGKVVTILGDTR
                     KCHASVRLAVNADVLVHEATYGKGDEKIARKHGHSTNMEAAQVAKDAGVKQLLLNHIS
                     PRFLSKDISQLRKDASTIFEQVHIVKDLEEIEL"
     gene            8257..9012
                     /locus_tag="ACHU66_001378"
     CDS             8257..9012
                     /locus_tag="ACHU66_001378"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFR3389656.1"
                     /translation="MRTILITGASGGLVQEMVPLLKDDFLILLGRDVAKIEQLYVHHE
                     KKAVFDVDIRDEMALTAFFDELDSQFGQIDILVNNAGYAIYDDFENFSSQQVQDMFDI
                     NTFALMTMCRLVGKRMKVRRSGQIINIISMSGLIASAKSSVYSATKFAAMGFSNTIRL
                     ELAQYGVTVTTVNPGPIATGFFGQADPDGSYQESVKAFLLQPDYVAKKIVSAMGTKKR
                     DINLPWSLAAAHKLYTLFPRIADYLASTVFNLK"
     gene            complement(9050..12235)
                     /locus_tag="ACHU66_001379"
     CDS             complement(9050..12235)
                     /locus_tag="ACHU66_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HFR3389657.1"
                     /translation="MKLSYKKRLLNQVLLASTVLFATSLAQGTVFANTEEIPPTTSET
                     VTPLPEETPITKTSTSEATDNLVEGKETEKQTEEIADTSPALVSTEEETTSSEPNAEE
                     TTLRSANNDNQDTTEEKSAVPTIDTVTLETKTVYLSEAVTITESVTLPDTTEKIEWTL
                     DGKPISEWKTWNLKEGDFTGDTFITVEESRQDNQLHLNIQLAALFGEDLSKRTPSNIR
                     RTYRHFIKDMLLEGTSADGNLIISKTLHFRPYEAYRTHEEMLTEIEETKNNAATDRLV
                     RIESIGQSAEGRDIKMAVVAKDQASIDKYLTETTPLMLTQPDQMLKQLQAGTFDYKLP
                     ILINNTHADEQPGIDVVTSLFKEFAQKDTITFPSTDADGNPVTLHLKVTDLLDKFIFL
                     FNFTENPDGDVKNLRSLVNGLDPNRDTGFQVNPETQAIVRQIHKWNPISVLDIHGFVS
                     GFLIEPATPPHDPNFEYDLLADIMLEKAHEMGRAGIANSKYERYTIPKVHWGDGWDDS
                     FSGYTAVYAMYHGILGHTIEIPEGNQESFKAGFFAVLGGVHNMATKPDSLMEMRLKYY
                     SRGVNKVEDPKAESELVGPDGAVVGRVKKDQPKFFPDYYVIPMTLDKHNDMQEAFKMI
                     EYFNRNGVVVKELTEDVGNFRKGDLVVDMAQAKRGFANHVLYAGSDESAWGAMYAELV
                     VNFPDMKGFRAKAVFEENAFSSKLGSITWTKAPRTTEIDFKAPYYVVTNTSESAVQAI
                     NQAIKSGAKVYLTDDGYILETNQFSHLLDTYALYGEPLYKKPIGQELKAMKVYAPSHS
                     YSWAGDFAILANAALAVERMGFEIVNSADEADAIILESDQFDASVFGKKPIIIVGGVA
                     MQKLEELGILAGFNAEQFTDGGDYEGLMRAIIDDKDPLTSGYAMNGLFYSNSGNWIEG
                     IPEGFKTLVKIADKDYYIAGWWPGHDKLANKIVAIAGNYQDQPVFIYAGNPTNKVHPV
                     HFFRWVSNALFGSQLATLEDLPAVEILVPQPEMPKNILDEAPKNTTVVHTTKSTEAKQ
                     LPQTGEKTNYIAIALGSLLLGSIALRRKERS"
BASE COUNT         3380 a         2505 c         2769 g         3650 t
ORIGIN      
        1 taatagagaa aagcacagga aaaggagcaa ctcttgctag aacattcaat tgatattcaa
       61 ctcaagcatc cagatgattc gtttagccta tttggttcga atgaacgcca tctgagattg
      121 atggaacagg aactaggtgt ggttattcat gcccgtacgg aaaaagtgca ggtgattgga
      181 caggaagaac atgtagaaca ggcacgtctt gttatccagt ctctccttgt cttggtgagt
      241 cgtggtttgg ttatcaatac accagatgtg gtcacagcta tttccatggc aaaaaatgat
      301 gagattgaga aattcgttgc cctctatgaa gaagagatta tcaaggacaa ctatggcaaa
      361 cccattcgcg taaagactct tggacaaaaa ctctacgtgg acagcgtaaa gcgtcatgac
      421 atcgtgtttg gaattggacc agctggtaca gggaagacct tcttggcagt tactctagct
      481 gtaacagccc tcaagcgggg acaggtcaag cggattgtcc tgacacgtcc tgcggtcgag
      541 gcaggcgaaa gtttaggctt tttaccaggg gacttaaaag aaaaagtaga cccatatttg
      601 cgtcctgtct atgatgccct ctaccagatt ttgggcaagg agcagaccac gcgcctaatg
      661 gagcgcgaga ttatcgagat tgcaccgttg gcctatatgc gggggcggac cttggaagat
      721 gcctttgtca tcttggatga ggcgcaaaat accaccatta tgcagatgaa gatgttcctg
      781 acccgtctgg gctttaattc caagatgatt gtcaatgggg atattagtca gattgacctg
      841 ccacgcaagg ttaagtcagg cctgattgat gcgacagaga aactaagcaa aattccgcag
      901 attgatttcg tacatttttc agccaaggat gtggttcgtc atccagttgt tgcccagatt
      961 atcaatgcct atgagcagga ggctctggct gcggatggtc gagcaagttt tgagacgatt
     1021 ggggagccta gtcggaaata caatggcgaa gagtagttgt ttcattggct gatatataaa
     1081 agaattgtga tgctagttac atttaatata caataaaaag cccaaatgga cgatactgta
     1141 agcgatgaaa caaacaggaa cttagtccaa ataagcgact tacagcatat cgtaaaatca
     1201 catcgtacag caaatatcaa ctatttgtca caagttatac caatcctatt gcccgacccc
     1261 cttaaagcat tgtcacaacg ttgctttgtc aacggtatca tataaatcta ttaaaatatt
     1321 gctagtactt caagttggat tagagattaa atcttaacga cattttcgaa ccagccctca
     1381 ttgtagcgga tagaggtcat aaaaactata actttatgga ttgttaccaa taaaaaggct
     1441 ggttctatct gattcggatt cgagatggga aaagcagcat gaaaccgagt tttactccct
     1501 ttcttttcgc atgttcggca ccgataagta tgaaaccctc gtggccaatt gtgataaacc
     1561 tgtagactat ataaaaagtc tccttttgtg ttgtccgaac ataaaatccc aagtattaca
     1621 tcggaaaaat acttgggatt agatatcata gattaactga tcagagttaa cttagagaca
     1681 ctgattgatg acagggcttt tatgcacgaa taacaagaag tggcttaaga ccaaaacttc
     1741 tctgcaattt cttgaccttg tttaattttt gcccactgtt gcgggatggc taattcatta
     1801 cctgaatcac atgaagcgaa tccacattga tgtgacaagt agagacgctc ttttggaata
     1861 atcttgcttg cttcctcgag taatttgtaa acacgttctt catcatccaa atcagaagtt
     1921 ttacttgaaa gtagaccaag aacgacttcg acattcttgt cacgcaagct ttccaaagct
     1981 tttaaatcac ctgcacgttc atcatcccac tcaaggaaga atcgatcgta tttttggtca
     2041 cgcaggaatt tttcggcaat agcttcgtaa gttccgccag ctgcagagcg gctttcatag
     2101 ttaccacggc agttgtgcgt ccagacggtc aagccaattt catgtccgta atcaataaca
     2161 gcattattga tagcaacaaa ctcgtcagcc aaagcggcca atgcttcaga atcgtcttgt
     2221 ggcaagaaag gtacagggtt agcagggtca aataattccc aaaggcagtc atcaaattga
     2281 acgatttatc caccagcttc cttgtactgg tctaaaaatt ccttataggc agcgatgagc
     2341 ccgactttta attcatcacg agtcttgtag acctgatttg gtccaacttg tccattgaaa
     2401 attgtcaatt cattaaaggc atgggctgga ccgaaaatag tttgtttggt tacagtttct
     2461 cctgctaatt ctttcacttg tttgaaaata tcaatgaaat ggtgattttt tgcagaaagt
     2521 ggagcagtaa tacgcaatcc aatatcctta cgtgtttcaa aaatctgtcc atcatggtct
     2581 ttaaatggat aaccatgctc agcaatgtaa cgctcgatac cgtcgaagcc ccagagaaaa
     2641 tctaggtgcc acattgaacg accaaattct ccatctgtca caatatccaa accgttttct
     2701 ttttgttctg caacaatttt ttggatgagt gaagcttcag tttctttgta accatcgaag
     2761 tcatcataga atgggtattt gatgtcttca cgagcttcga tttgtcgttt aaattctcct
     2821 aagtttgctg gtcgaagaag tgaaccgact aattggaatc ttgaagttgt catatatgta
     2881 tctctctttc tttcgtttta gtgatgaacc tattctaact cataagctat catttgaaaa
     2941 atagttattt tcatggcaaa tccatagtta aaaactataa cttgaaaaaa tagaaggaaa
     3001 taactttcta tcatttgtca actatggtat aatcaaaagt gctgaaatcc gaacattagg
     3061 agacttagga gaaaaatgaa aaaacaatca ccatttataa ttgcaggaat cgtcatgcta
     3121 ggggtcgtga tgcgtgcccc ctttacagct ttgcctgcta ttttgataga tgtggcagca
     3181 ggactgagag tagaagtgag ttcattagga attttaacct ctattccgct catcatgttt
     3241 gctctctgtt catccttagc accgcgtctt gcggctaagt ttggcatgga aaaactaatg
     3301 gccctagtct tactggttat ggtgctgggt tcaggaatgc gagttctcaa cttaccagcc
     3361 ctttacattg gtacaatgct tgttggtgct acaatagcat ttattaatgt tttgctacca
     3421 agtttggttg cggctaattt tccaaagaaa attggtctgt atacgacgat ttatatcact
     3481 ctcatggggg tggcggcaac agttgcatct atgattgctg ttccaattgt atcctctagt
     3541 tcttgggaat tttttattct attaattaca ggattggtgt ttatggcttt cctaatctgg
     3601 ttgcctaatg taagaaataa ccatcgattt gcaagtgaga accaaggaaa ccagaaatca
     3661 tctatctgga aaaataaagc agcgattgcc tttttgatat ttggtgggct acaatcagtc
     3721 ctctactata cagagattac ctggttgcca actatttctc aatcagttgg ctttagtaag
     3781 gcagaagcag ggctaatggc tggcttcttc aatatgacag ctattcccat gtctatgatt
     3841 atcccagctg ttctttcccg tcagacaaaa gaaatgcgtc gaaatatcat gctggctatt
     3901 tcatctgtta ccttacttgg tttggtcatg atggtattga ttccgaccaa tctcattctt
     3961 tggtcagcgc ttcacattat tttaagtttt tcaaatgcag ccctcttccc ttatatgatg
     4021 ttgagcttta ctttgaaaac aagcaacagc caggctactg cccagttatc aggaatggtg
     4081 cagacaggtg gctatctcat tgcagcattt ggaccaggcc ttctcggtta cagctatcca
     4141 atcttcggaa actggatgcc attgattctt gctctggcta tcgtcacact tgccatgatg
     4201 tggactattg ttctcattga aaaagaagat attatcttat aaagtataat cccttaagaa
     4261 ggctggcatt tttctgtgaa aatggcagag cttctaaggg attttttgtc aaacaaaaaa
     4321 gctagacatt tgccagcttt ttaatgtgcc acacggcgac gtgcagcttt tttgcgattt
     4381 tcttcgatga aagcttcttt cttttcttct ggttcaatga ttgttttgtg aacagtgaag
     4441 actgcgcctg ctgcaagagc aactgtagaa agaacacctg tcaagaaacc tttaccaaaa
     4501 cctttagcca tagttatttc tcctttactt gtgttataat agattttagc gaaaaaacaa
     4561 aatattttca aggaaaaaga tgaaaactaa agttattgtg gtcattggac cgacagcggt
     4621 aggaaaaact gcccttggca tagacttggc ccagcgctac aatggagaaa ttattagcgg
     4681 tgatagccag caagtctatc gcaaacttga tattggcacg gcaaaagcta gtcctgaaga
     4741 gcaggctgct gcggttcatc acttgattga tgtccgagat gtgaccgagg gctattcggc
     4801 ttatgaattt gtagcggaag ccaaggcctt aattgcagac attactagtc gtggaaagtt
     4861 gcctattatc gtgggtggga cagggcttta catacaaagt ctgttagaag gctaccatct
     4921 gggcggtcaa gttgaccagg tgcaagttct tgcttatcgg gcagaacttg atagcttgtc
     4981 tgacgaggat ttggaaacaa tggcagagca ggcaggtttg atggtcgagg gaaacagccg
     5041 acggagaatc attcgtggac tggaattgaa aaaatttggc gaaaacttag aaaatacaga
     5101 gtcaggatat gagccacttt acatctgttt gacggatgac agacaggttc tttacgaccg
     5161 catcaatcag cgagtggata agatgatggc ggcgggctta ctggatgaag ttagctggct
     5221 ctaccaagaa catccccaag cccaagcagc catggggatt ggctacaaag aatttttccc
     5281 ttatcttgca ggagccattt cgctggaaga ggctattgat aaggtcaagc aaaacagccg
     5341 ccgctttgcc aaacgccaat tgacttggtt cagaaatcgc atgcaggttc ccttctattc
     5401 agtaggcgag ccagattgca agtcgcagat tttcaccgcc gtggaggaat ttttaaatga
     5461 ttgaaacgca gaaagaacaa gaacgcgccc tcctggttgg agtggaatta cagcagacag
     5521 acaactttga catgtccatg gaggagttgg caagtcttgc taagacggct ggtgctctgg
     5581 tcaagggagt ctacactcaa aaacgagaaa aatacgacag caagactttt gtcggttctg
     5641 gtaagctgga cgaaattgcc cagatggtcg aagctgacga gattgatact gtcatcgtca
     5701 acaatcgatt aacaccccgc cagaatgtca atctggaaga aattctaggt gtcaaggtaa
     5761 tcgatcgcat gcagttgatc ttggatattt ttgccatgcg ggcacggagt cacgaaggga
     5821 aattgcaggt ccacctagcc cagctcaagt acatgttgcc acgcttggtt ggtcagggca
     5881 tcatgctcag ccgtcaggca ggtggtatcg gctctcgtgg accaggtgaa agccagttgg
     5941 agcttaaccg ccggagcatt cgtaaccaga ttcatgacat tgagcgccaa ctcaaaacag
     6001 tggagaagaa ccgagcgact gtccgtgaac gccgtttgca atcaggcgtg ttcaaaatcg
     6061 gcttgattgg ctataccaac gcgggtaagt ccactattat gaatgtcatg acggacaagc
     6121 gacagtacga ggctgatgaa ctttttgcca cactagatgc cacaaccaag cagattaact
     6181 tggcagacaa atttaatgtg accttgaccg atacggttgg tttcattcag gacctgccga
     6241 ctgagttgat ttctgccttc aagtccacct tggaggagtc catgaacgtg gatctcttgc
     6301 tccatatcat cgacgcctct gaccccaatc acagtgagca ggagcaggtg gtcttggaca
     6361 ttctaaaaga cctggatatg ctggacattc cacgtctagc tctctacaac aagttggaca
     6421 agaccgatga cagctttacc cccagtcagt tcccacatgt catgctgtcc gctaaggatg
     6481 aaaatgccaa aggtcatatt cagatgatgg tcttggccaa gattaagacc atgtttgaac
     6541 gatttgaggt cagggttcca cttgctgaat cttataaatt gcatgattta gcaactcttg
     6601 ccctgataga aaatcggacc tatgaagagg atgtggaagt ggtatcaggc tatatcgctt
     6661 ccgctaataa atggaagttg gaagaatttt atgacggatt atcttgattt ggcaatgaaa
     6721 tacggtggct ttaccagtct ggacaaggtc tatctggcga ataaattagc tggtttgaca
     6781 gaccaacaaa aactagattt tatcacgcca ccgccttcgg ttatcaatgc ctattttgcg
     6841 gaaatctacc agaaacaagg ggcagaagag gcgacggact actatctcac tctttctcag
     6901 cagctctgcc tttttccaaa agaacccagc tttgctgaaa gcaagccctt tgtccgcctg
     6961 aatttatcag gtaagtcttt cggctttacc tatgtgtcgg cggacggtct ggcccaggtt
     7021 ttttcggaag gtgtagagga agtgacggaa agtttgctct ttgaaatcgc ccaagtcttt
     7081 ccccactatg tcgtctttgt ggaggagggc aagattttca tgaaagtcaa tccctttgag
     7141 gacgccgagt tagaggacgt tgagacagac taccttttga cccaagtggc gacgacagaa
     7201 ggtttggtca agatttcagg ctttaatcag gaagaagtgc tggaggtggc ggacggctat
     7261 gctggtcagt attactatgt ctggtcgggt cgctctgcaa ttttatatat aaaacattag
     7321 aaagtaaaca atgcaaattc aatttttagg tacgggggct ggtcagccct ccaaggctcg
     7381 aaatgtatcc agtttggcct tgaaactctt ggatgaaatc aatcaggttt ggctctttga
     7441 ttgtggtgaa ggaactcaaa atcaaatttt agaaacgact attcgtccac gtaaggtcgc
     7501 aaaaatcttt atcactcacc tgcacggaga ccatattttt ggactgcctg gttttttgtc
     7561 tagccgttct ttccagtcta gcgatgagca gacggatatt gatatttacg gtcctgttgg
     7621 catccgttcc tttgtcttgg caagtttgaa ggtatcaggg acacgcctac cttatcgcat
     7681 tcattttcat gagtttgatg ttgatacggt cggccaggtc ctagaaacgg acaaattcac
     7741 ggtattcgct gaaaagttgg accataccat tccgtgtgtc ggctatcgtg tcatccagaa
     7801 agatttggaa gggacattgg atgcagaggc tctgcgcgcg gcaggtgtgc catttggtcc
     7861 cttattcggt aaaatcaaaa atggtcaaaa cgtgacgcta gaagatggga ctgaaattat
     7921 cgcgagtgac tatatttctc ctcctcggcc tggtaaggta gtgaccattc ttggtgatac
     7981 gcgcaagtgt catgctagtg tccgtctggc tgtcaatgct gatgtgctgg tgcatgaggc
     8041 gacttacgga aaaggcgatg agaagattgc tcgcaagcat ggccattcga ccaatatgga
     8101 agccgctcag gttgccaagg atgctggtgt caagcagctc ttgcttaatc atatcagtcc
     8161 acgattttta tccaaggata ttagtcaatt acggaaagat gcaagtacta ttttcgagca
     8221 ggttcatatt gtcaaggatt tagaggaaat tgagctatga gaaccattct aatcacaggt
     8281 gcctctggtg gcttggtaca ggaaatggtg ccattgctga aagatgattt tttgatttta
     8341 ctaggtcgtg atgtggcaaa aattgagcag ctctatgttc atcatgaaaa aaaggctgtc
     8401 tttgatgtgg acatccgaga tgaaatggcc ctgacggcat tttttgatga actggatagt
     8461 cagtttggtc agattgatat tttagtcaat aatgctggct atgccattta tgatgatttt
     8521 gagaattttt ccagccagca ggtgcaggat atgtttgaca tcaatacttt tgctctgatg
     8581 accatgtgcc gtttagtagg taaacgaatg aaggtaagac ggagcgggca gattatcaat
     8641 atcatttcca tgtcaggctt gatcgcttct gccaagtctt cggtctattc ggcgaccaag
     8701 tttgcagcta tgggtttttc aaataccatt cgcttggaat tagcccagta tggtgtgaca
     8761 gttacaactg ttaacccagg acctattgcg actggctttt tcggtcaggc agatcctgac
     8821 ggaagctatc aagagagcgt caaggctttc ttgctgcaac cagactatgt tgccaagaag
     8881 attgtttcgg ctatggggac gaaaaaacga gacatcaatt tgccttggtc actagcagct
     8941 gctcataaac tctatactct ctttccgaga atagcagact acttggccag tacagtattt
     9001 aatttgaaat agaaaaacgc cgaaataaaa ctcggcgttt tcgtgtagac tagctacgct
     9061 cttttctacg taaggcgata ctgcctagaa gtaggctacc taatgcgata gcgatatagt
     9121 ttgttttttc acctgtttga ggtagttgtt ttgcctcggt agacttggtg gtatgtacta
     9181 ctgtagtatt ttttggtgct tcatccaaaa tattttttgg catttcaggt tgtggtacaa
     9241 gaatttctac agctggtaag tcttctaagg ttgctaattg actaccaaat agggcgttgg
     9301 acacccaacg gaagaagtga acagggtgga ccttgtttgt tggattacct gcatagataa
     9361 agacaggttg gtcttgataa tttccagcga tggcgacaat tttattggct aatttgtcat
     9421 gaccaggcca ccaacctgcg atataataat ctttatcagc gattttaaca agcgttttaa
     9481 atccttcagg aataccttca atccaattac cggagttgga atagaaaagt ccattcatgg
     9541 cgtagccact tgttaatgga tctttatcat caataatagc ccgcatgaga ccttcatagt
     9601 caccaccatc tgtaaattgt tccgcattga aacctgctaa gattcctaat tcctccagtt
     9661 tttgcatggc gacaccacct acgatgataa ttggtttctt accaaataca ctggcatcga
     9721 attggtctga ttcgagaatg atagcatcag cctcatctgc gctattgaca atttcaaagc
     9781 ccattcgttc tacagcaagt gcagcatttg caagaatggc aaagtcgcct gcccaactat
     9841 aagagtgaga aggagcatac accttcattg ctttcaattc ttgtccaata ggcttcttat
     9901 agagaggctc gccgtataga gcataggtgt ccagtaagtg actaaattgg ttagtttcca
     9961 agatgtagcc atcgtctgtc aggtagacct tagcgcctga tttgatggct tgattaatgg
    10021 cctggacagc gctttcagag gtgtttgtga cgacataata aggcgctttg aaatcaattt
    10081 ccgtagtacg tggagctttt gtccatgtga tgctacctaa tttgctgcta aaggcatttt
    10141 cttcaaatac agccttggct ctaaagcctt tcatatctgg gaagttgacc accaattcag
    10201 catacatggc tccccaggca gattcatcag aaccagcgta gaggacatgg ttagcgaatc
    10261 ctcgtttggc ttgagccata tcgacaacta ggtcgccctt gcggaagttt ccaacatctt
    10321 cagttaattc cttgacaaca accccattac gattgaaata ttcaatcatc ttaaatgctt
    10381 cttgcatatc gttgtgttta tcgagcgtca taggaatcac atagtagtct gggaagaatt
    10441 ttggctggtc ttttttgaca cgtccgacaa cagctccgtc tggtccgact agttcagact
    10501 ctgcttttgg atcttctacc ttattgacac cgcgtgaata gtatttgaga cgcatttcca
    10561 taagactatc tggcttggtt gccatattgt gaacaccgcc caaaacagca aagaatcccg
    10621 ccttgaatga ttcttgattt ccttctggaa tttcaatcgt gtgtccaaga ataccgtggt
    10681 acattgcata caccgctgta tagcctgaga aggaatcgtc ccaaccatct ccccagtgga
    10741 ctttagggat ggtgtagcgt tcatatttag aatttgcaat acctgctcgg cccatttcat
    10801 gagctttttc cagcataata tcggctagta aatcatattc aaagttcggg tcgtgtggcg
    10861 gagtagcagg ctcgatgaga aatccagata caaaaccatg aatatctaaa acagatattg
    10921 gattccattt gtggatttga cggacgatgg cttgtgtttc tggattcact tggaaaccag
    10981 tatcacggtt agggtccaaa ccatttacta gtgaacgcaa gtttttaaca tcgccatcag
    11041 gattttctgt gaaattgaac aagaagataa atttatctaa caaatctgtc acttttaaat
    11101 gaagagtaac aggatttcca tcggcatctg tagatgggaa ggtgatggta tctttttgtg
    11161 caaattcttt gaataggcta gttaccacat ctatgccggg ttgttcatca gcatgagtgt
    11221 tgttgatgag aataggtaat ttatagtcga aggttccagc ttgcaactgt ttcagcatct
    11281 ggtcgggttg ggtcagcatg agtggtgttg tttcagtaag atatttatca atacttgctt
    11341 gatctttagc cacaacagcc attttaatgt ctctaccttc agctgattga ccaattgact
    11401 caatccttac taggcggtcg gtcgcagcat tatttttcgt ttcctcaatc tctgtcaaca
    11461 tttcctcatg tgtacggtag gcttcataag gacggaaatg aagagttttt gaaattatca
    11521 agtttccatc ggcactagta ccttccaaca gcatatcttt tataaaatgt cgataggtac
    11581 ggcgaatatt actcggtgtc cgtttactca aatcttctcc aaagagagct gcgagctgga
    11641 tattcaaatg aagttggtta tcctgtctgc tttcttctac tgtgataaat gtatcgcctg
    11701 tgaaatctcc ctctttcaaa ttccaggttt tccattcgga aataggcttt ccatccaatg
    11761 tccattctat tttttcagtc gtatctggaa gtgtgacgga ttctgtgata gtgactgctt
    11821 ccgatagata gacagttttt gtttcgagtg taactgtatc aatagtcggt acggcagatt
    11881 tttcttctgt agtatcctga ttatcattat tagctgatct gagcgtagtt tcctcggcat
    11941 tgggctcgga agatgttgtc tcttcttctg tggaaacaag tgcaggagaa gtatctgcta
    12001 tttcttcagt ttgtttttct gtctccttcc cttctacaag attatctgta gcttcgcttg
    12061 tgctagtttt cgttatagga gtttcttcag gcagtggagt cactgtttca ctagtagtag
    12121 gtggtatctc ctctgtattt gcgaagaccg ttccctgagc gagagatgta gcaaaaagaa
    12181 ctgttgaggc aagtaggact tgattcaaca gccttttctt atatgaaagt ttcatgcgaa
    12241 cttcctcctt ttgataatgt gtacggggta ataaattaaa acgggttatt tcatgcttat
    12301 ctta
//
LOCUS       DAZQCJ010000057        12132 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000057 DAZQCJ010000000
VERSION     DAZQCJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12132
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.058"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            114..1118
                     /gene="ccpA"
                     /locus_tag="ACHU66_001380"
     CDS             114..1118
                     /gene="ccpA"
                     /locus_tag="ACHU66_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFR3389658.1"
                     /translation="MLNTDDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLE
                     VIDRLDYRPNAVARGLASKKTTTVGVVIPNIANAYFATLAKGIDDIADMYKYNIVLAN
                     SDENDEKEINVVNTLFSKQVDGIIFMGYHLTDKIRAEFSRSRTPIVLAGTVDLEHQLP
                     SVNIDYAAASVDAVNLLAKNNKKIAFVSGPLVDDINGKVRFAGYKQGLKDNGIEFNEG
                     LVFESKYKYEEGYALAERILNAGATAAYVAEDEIAAGLLNGVSDMGVKVPEDFEIITS
                     DDSLVTKFTRPNLTSINQPLYDIGAIAMRMLTKIMHKEELENREVVLNHGIKVRKSTK
                     "
     gene            complement(1550..3379)
                     /locus_tag="ACHU66_001381"
     CDS             complement(1550..3379)
                     /locus_tag="ACHU66_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FctA domain-containing protein"
                     /protein_id="HFR3389659.1"
                     /translation="MNTKKWRTSLLIPGIVLFGTVALVNNVSAQEVKNTIISAKQPDG
                     RQATSKAVNVKIPAVVRLFGRELLENEFKFELREANSEKLIDTTQNTKEGQVSFKNLS
                     FNKPGEYWYTISEVKNELSGVEFDSKYIVAKITVEDRNNGQLQAMIEFIADDNVFNNF
                     YTPAPAAASLSIKKVLEGRTLNTGEFEFVLKNEKGDEIEKVSNQADGSVNFSALTFTK
                     EGTYTYTVSEVDGGLGDIIYDKSDIKATVTVKDNNHGQLVSTVTYENSDQIFENILNP
                     GKLITPTTDSVITDNEVSKEAMTDKEKGNIEPPKEQIATEEKDNIEASEKQMPSIVND
                     MVVTPEKQMTNKENDNVETSEKQMPSVMNENAVTPEKQMTNKENDNIETSEKQMPSVM
                     NENAVTPEKQMTNKEKDNIETSEKQMPSVVNENAVTPEKQMTNKENDKVVISEKQMPS
                     IVNDMVVTPQEQMANKENDEVVISEKQMPSIVNDMVVTPQEQMANKENDNVETSEKQM
                     PVNEKDNAVTPERQIANKEKENIETSKKQIPVNGNNQNGTVGENSNTKPTTEKTDKQE
                     TSTFKTETAKQILPVTGEKGSLWLLTSGIIGLAIALFTRKRKL"
     gene            3689..4687
                     /locus_tag="ACHU66_001382"
     CDS             3689..4687
                     /locus_tag="ACHU66_001382"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR3389660.1"
                     /translation="MKVLLYLEGKTVLEKSGIGRALQHQMEALDMAGIPYTTDLLGDY
                     DVIHINTYGPRSWLLLHAAKRSGKKVIMHGHSTKEDFQNSFIGSNTLAPFVGKYLASM
                     YQKADFVITPSEYSKKLIQSYGVTTPIIAVSNGIDLDKYKKDERKEAIFREHFKIEEG
                     QPVVVCAGLYFRRKGIEDFVKVAEKMPHVRFIWLGSINKWLIPSYIRKIVEGDHPANV
                     EFPGYFKGAVFQGAMSGADAFFFPSYEETEGIVVLEALASHQHVVLRDIPVYDGWVDE
                     NVAELGHTVDDFVDSIQKILDKQVDKREAGYQVAVSRSIDNVSHQLVDAYRQVLEM"
     gene            4688..6001
                     /locus_tag="ACHU66_001383"
     CDS             4688..6001
                     /locus_tag="ACHU66_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR3389661.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKLGHTVFIFTTTDED
                     VNRYEDWDIIRIPSVPFFAFKDRRVAYAGFTDALKIASRYKLDIIHTHTEFSLGILGK
                     MIAKELDIPVVHTYHTQYEDYVHYIAKGRIIRPGMVKYLVRAFLKDLDGVICPSEIVE
                     DLLMSYNVPISKRVIPTGIDLAKFDRPEIGKDETDELREQLGIASDETMLLSLSRISH
                     EKNIQAIVQAMPKILLENPKVKLVIVGGGPYTDDLQEMIAELNLDEHVQLTGMIAPSD
                     TALYYKAADFFISASTSETQGLTFLESLASGTPILAHSNPYLKNVVTDKMFGTLFVRE
                     QELADAVVEAIVATPPMNPYVLDKKLYEISSTNFGRKVFEYYLDLKISYDFRKEHTNQ
                     DSVAEALIKEAIYLPQKVVVKSTDTTAKILKKSVEQVKSIRNFFE"
     gene            6016..6390
                     /locus_tag="ACHU66_001384"
     CDS             6016..6390
                     /locus_tag="ACHU66_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389662.1"
                     /translation="MEKLRKESWKNLVLATLIILSMYAGIYLIAFHLISDTMLSKELV
                     VGNILLYTPIVLFLTGLLYGFFHGFSPSLLALVLLLYPVTIFFFKEWIILYQIVYTLC
                     ALIGNGVGALLFSLRKKMRKNP"
     gene            6654..7025
                     /locus_tag="ACHU66_001385"
                     /pseudo
     CDS             6654..7025
                     /locus_tag="ACHU66_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422773.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
     gene            7043..8992
                     /gene="thrS"
                     /locus_tag="ACHU66_001386"
     CDS             7043..8992
                     /gene="thrS"
                     /locus_tag="ACHU66_001386"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR3389663.1"
                     /translation="MIKITFPDGAVREYQAGVTTFEIAESISKSLAKKALAGKFNGKL
                     IDTTRAIEEDGTLEIVTPDHEDALDILRHSAAHLFAQAARRLFPDIKLGVGPAIQDGF
                     YYDTDNAAGQISNEDLPRIQEEMMKIVKENFPSERREVTKEEAIEIFKNDPYKLELIQ
                     EHSDDEGGLTIYTQGEYIDLCRGPHVPSTGRIQIFELLNVAGAYWRGKSENPMMQRVY
                     GTAWFDKKDLKAYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRT
                     LERYITDKELTSGYQHVYTPPLASVELYKTSGHWEHYSEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVYKNHVRSYRELPVRIAELGMMHRYEKSGALTGLQRVREMTLNDGHIFVTP
                     EQIQEEFKKALQLIIDVYADFNLNDYRFRLSYRDPEDKEKYYDNDEMWENAQRMLKGA
                     MDEMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFGLTYIGADG
                     EEHRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPTQVTMIPISVEAHLDYAWKVA
                     KELQDRGVRVHVDERNEKMQYKIRQSQTSKIPYQLIVGDKEMEDNAVNVRRYGSKATQ
                     TQSVAEFVDHILADIARKSRPAEAE"
     gene            complement(9064..9723)
                     /locus_tag="ACHU66_001387"
     CDS             complement(9064..9723)
                     /locus_tag="ACHU66_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR3389664.1"
                     /translation="MNYVLELLKPSTFQLLWNGLKLTLYISSISVILSILFGMILAIM
                     RNGKNPIFKWIATIYIEFVRNVPNLLWIFTVFLVFQMKSTPAGITAFTIFTTAAMAEI
                     IRGGLNAIGPGQTEAGLSQGFTPVQIMVYIIMPQAIRKMLPAIISQIVTVIKDTSFLY
                     SVIALQELFGSSQILMGRYFEAEQVFALYLMVALIYFSINFAISSLSRYVAKSWASSI
                     E"
     gene            complement(9738..10436)
                     /locus_tag="ACHU66_001388"
     CDS             complement(9738..10436)
                     /locus_tag="ACHU66_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR3389665.1"
                     /translation="MTVLTTSPYAWENWVSYFKDFPTFFQGFLFTLAISVGAFIMAMF
                     LGIVFGSLSSSKNKVLKIIARVYVEYYQNTPLLVQFMIVYYGLPLISNYVLMPSIYWT
                     AVICVGLYHGAYIAEVIRAGIEAVLTGQTEAALSQGFTQSETMRIIILPQAIPTILPP
                     LTNQVVNLIKNTATVAIISGADIMFMTKSWSAMNANYIPAFAGAAFLYFIMCFPVASW
                     GRRIEEKNKSAFSH"
     gene            complement(10451..11290)
                     /locus_tag="ACHU66_001389"
     CDS             complement(10451..11290)
                     /locus_tag="ACHU66_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFR3389666.1"
                     /translation="MDKKKINIFFAIRLLVFGLILFVFAKQPVAQTETPASITNSDQV
                     QAIIDRGVLRVGVKQDVPNFGYKNPDTGEFEGMEIDIARKIADELGVDIEFTPVTAQT
                     RGPLLDNGQVDMVIATFTITEERKLLYNFTTPYYTDAVGFLVNKDSGIKTFTDLDGKT
                     IGVAQGSITRTLISKLADKYGISVNFAELGSYPELSVSLRAHRTDAFSVDQSILSGYI
                     SSKSELMDFSFSASDYGIVTKLSNKDLNNYLNSLVEKWTADGSLQSIYDANGLKPVTE
                     TDE"
     gene            complement(11301..12062)
                     /locus_tag="ACHU66_001390"
     CDS             complement(11301..12062)
                     /locus_tag="ACHU66_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR3389667.1"
                     /translation="MALVEFKDVNKYYGDYHALRNINLEFEPGQVVVLLGPSGSGKST
                     LIRTINGLEAIDEGTLIVNGHDISSTSIKELVSLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKATAEKTAQKYLEFVNLWDRKDSYPAMLSGGQKQRVAIARGLAMKPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDGMNMIVVTHEMGFAREVADRIIFMAEGEVL
                     VDTTDVNGFFDNPTEPRAQQFLSKIINHESDKVKL"
BASE COUNT         3502 a         2319 c         2652 g         3659 t
ORIGIN      
        1 atcaaaaaaa tagagtttat ctcttgaaag tggtttcaaa aagtgataca ctatacttga
       61 gtgaaagcgt aattttcata gaactagaac tattgaaagg gagtacataa cgtatgttaa
      121 acactgacga tacggtaacg atttatgacg ttgcccgcga agcaggtgta tccatggcga
      181 cagtatcgcg cgtggtaaat gggaataaaa acgtaaagga aaatactcgt aaaaaagtat
      241 tagaagtcat cgaccgtttg gattatcgtc cgaatgctgt tgcacgtggc ttggccagca
      301 agaagactac cactgtgggg gttgtgattc caaatattgc taatgcttat tttgcaacct
      361 tggccaaagg tatcgatgat attgccgata tgtacaaata caatatcgtc ttagcaaaca
      421 gtgatgaaaa tgatgagaaa gaaatcaatg tggtgaatac cctattctca aaacaggtgg
      481 acggaatcat tttcatgggc tatcatttga cagacaagat tcgtgcggag ttttcacgct
      541 cacgtacacc gattgtttta gctggtaccg tggacttgga gcaccaatta cctagcgtca
      601 atattgacta cgctgccgct agtgttgatg cagtcaatct attagctaag aacaacaaga
      661 aaattgcctt tgtatcaggg ccgcttgtag atgacatcaa cggtaaagtt cgttttgcag
      721 gctacaaaca aggcttgaag gacaacggaa tcgagtttaa cgaaggattg gtttttgaat
      781 ccaagtataa atacgaggaa ggctacgctc tagcagaacg tattttgaat gccggagcaa
      841 ctgcagctta tgttgcagaa gatgagattg ctgctggttt attgaatggt gtcagtgata
      901 tgggcgtcaa ggttccagaa gactttgaaa tcattacaag tgatgattcc ctagtgacta
      961 agtttacccg tccaaacctg acctctatca atcagccact atatgatatt ggtgcaattg
     1021 ctatgcgcat gctcaccaaa atcatgcata aggaagagtt ggaaaaccgt gaggtagttc
     1081 ttaaccacgg aatcaaagta cggaaatcaa ctaagtgata aatatgggag tagccggata
     1141 agatatctta ttactccgca ccctcactct atcaaacatt ataatcattt ggtttatctt
     1201 ttattacttc gacgagcgag gaaatatttc caacctagaa cagcctttag gctgttctag
     1261 caacttgtag ctattgaggc aaacttgctt cgacttatct ccagccttca aaggttcgga
     1321 gaacctttga agcgagtact aaaagcaaac aaaatggcta tactaaccga aaactaaaag
     1381 attataaaaa ataaatataa agccctcgct agatgtataa gcttagcgag ggctttgtgg
     1441 ttgattttaa gtctgtcaca ctctgtaagt tgtgcgagat gttgatgcga atctagagaa
     1501 gcagtactgg gcttgttctc atcctttaga actgtatcag ttaggtgaat tataatttac
     1561 gtttacgtgt aaacaacgca attgcaagcc cgataatacc acttgtcaat aaccataaac
     1621 ttcctttctc accagttact ggtaagattt gcttagcagt ttcggtttta aatgttgaag
     1681 tctcctgctt gtctgttttt tcagttgttg gtttagtgtt tgaattttct ccgactgtac
     1741 cattttggtt gttcccatta acaggtatct gttttttaga ggtttcgata ttttccttct
     1801 ctttattagc tatttgcctt tcaggtgtta cggcattgtc cttctcatta acaggcatct
     1861 gtttttcaga ggtttcaaca ttatcgttct ctttattagc catttgttct tgaggtgtta
     1921 cgaccatgtc gttcacaatg cttggcatct gtttttcaga gattacaact tcatcgttct
     1981 ctttattagc catttgttct tgaggtgtta cgaccatgtc gttcacaatg cttggcatct
     2041 gtttttcaga gattacaacc ttatcgttct ctttattagt catttgcttt tcaggtgtta
     2101 cggcattttc gttcacaaca ctcggcattt gtttttcaga ggtttcaata ttatccttct
     2161 ctttattagt catttgcttt tcaggtgtta cggcattttc gttcataaca ctcggcatct
     2221 gtttttcaga ggtttcaata ttatcgttct ctttattagt catttgcttt tcaggtgtta
     2281 cggcattttc gttcataaca ctcggcatct gtttttcaga ggtttcaaca ttatcgttct
     2341 ctttattagt catttgcttt tcaggtgtta cgaccatgtc gttcacaatg cttggcatct
     2401 gtttttcaga ggcttcaata ttatccttct cttcagtagc tatttgctct ttagggggtt
     2461 cgatatttcc cttctcttta tcggtcattg cttccttaga gacttcatta tcagtaataa
     2521 cgctatccgt ggttggcgtt attaacttcc caggattcaa aatattctcg aagatttgat
     2581 cgctattttc ataagtcact gttgagacta gttgtccgtg attgttatct ttcacagtga
     2641 cagtggcctt aatatctgat ttgtcataga taatatcgcc aagtccacca tcaacttctg
     2701 aaacagtgta ggtataggtt ccctcttttg taaatgttag ggcactaaag tttacagaac
     2761 catctgcttg attgcttacc ttttcgattt catcgccttt ttcatttttt aaaacaaatt
     2821 cgaattcacc ggtgtttaag gtacgtccct cgaggacttt tttaatcgaa agactagcag
     2881 cagctggagc aggtgtatag aaattgttaa agacattgtc atcagcaata aattcaatca
     2941 ttgcctgtaa ttgtccgttg tttcgatctt ctacagttat ttttgctaca atatatttcg
     3001 aatcaaactc aacaccacta agctcatttt ttacttctga aattgtatac caatattcgc
     3061 cgggcttatt gaatgataga tttttaaaac taacttgacc ctcttttgta ttttgagttg
     3121 tatcaataag tttctcacta ttcgcttctc taagctcaaa tttaaattca ttttctagaa
     3181 gctcacgacc aaatagtcgt actactgctg gtattttgac attaaccgcc tttgaagtag
     3241 cctgtctccc atcaggttgt tttgcgctga tgatggtatt ttttacttct tgtgccgata
     3301 cattattcac tagagcaaca gttccaaata atactattcc tggtattagg agcgatgttc
     3361 tccatttctt agtattcatt atcctttttc ctttctcaaa gcatgattta tgtagctaat
     3421 ctgtgtcgtt ataacatgca aattttacat ctaaagtcct caataaattt tgaacgctta
     3481 gtgccagaat gtgcaccatg cgtaaattat aacatcttct aaaaaacatt caagctgtcc
     3541 ttctgttttt aaaatctgtt ttatatgttt tttatgtaat catatatttg ggattatatt
     3601 ctttagtatt ggctttaatt gatacaaaat tcgtgtagaa tacagtttaa aatatgaaaa
     3661 aaatgtaaaa aagggctata atggtattat gaaagtacta ttgtatttag aaggtaaaac
     3721 tgttttagag aaatcaggaa ttggccgggc acttcaacac cagatggaag cgctagatat
     3781 ggcaggcatt ccttatacaa cagacctgtt gggagattac gatgtcattc atatcaatac
     3841 ctatggtcca cgctcctggt tattgctaca tgcagccaaa cggagtggaa aaaaggtaat
     3901 catgcatgga cattccacga aggaagattt tcaaaattcc tttatcggtt cgaataccct
     3961 agctcctttt gtagggaaat atctagcaag catgtaccaa aaggcagact ttgtcatcac
     4021 tccttcagag tattcaaaaa aactcatcca gtcttacggt gtgacaacgc caattatagc
     4081 cgtttcgaat ggtattgatt tagataaata taagaaggat gaacgcaagg aagcaatctt
     4141 tcgagagcat tttaaaattg aagaagggca gccggttgtt gtctgtgcag gactctactt
     4201 ccgacgtaaa ggaattgaag actttgtcaa ggtagccgaa aagatgccgc atgtccgctt
     4261 catttggctc ggttcaatca ataagtggct gattccgtca tatattagaa aaattgttga
     4321 gggagatcat ccagcgaatg tcgagtttcc aggttatttt aaaggtgctg tttttcaagg
     4381 tgcaatgagc ggagcggatg cctttttctt cccatcgtat gaggaaacag aagggattgt
     4441 tgttctggaa gctcttgcta gtcatcagca tgttgttcta agagatattc cagtttacga
     4501 tggttgggtt gacgaaaacg ttgccgaatt gggacataca gtggatgatt ttgtcgattc
     4561 tattcagaaa atcttagaca agcaggttga taaacgtgag gcgggctacc aggttgcggt
     4621 tagtcgctcg attgacaatg tttcccacca attggtggat gcctatcgac aagtattgga
     4681 gatgtaaatg cggattggat tgtttacgga tacctatttt ccacaggttt caggagtcgc
     4741 gacttcgatt cggactctta agacagagtt ggagaaattg ggacatacgg tatttatttt
     4801 tacgacgaca gatgaggatg tgaatcgcta cgaagattgg gacattatcc gaattcctag
     4861 cgtacctttt tttgccttta aagatcgtcg agtggcctac gcaggtttta cagatgcctt
     4921 aaaaattgca agtcgctata aactagatat tattcatact catacagaat tttctttggg
     4981 gattttgggg aaaatgattg ccaaggaact agatattccg gtggtacata cttatcacac
     5041 ccagtatgaa gactacgtac actatattgc caagggtagg attattcgtc ccgggatggt
     5101 taaatattta gtacgggcat ttttaaaaga tttggatggg gtaatttgtc ctagtgagat
     5161 tgttgaagat cttttaatga gttacaatgt tccgatttct aaacgagtaa ttccgacagg
     5221 aattgacttg gcaaagtttg atagaccaga aattggcaag gatgaaacag atgagttgcg
     5281 ggaacagcta ggaattgcta gtgatgaaac gatgttgtta agtctgtcta ggatttctca
     5341 cgagaagaat atccaagcaa tcgttcaggc aatgccaaaa attttgctag aaaatccaaa
     5401 ggtgaagttg gtcattgtcg gaggtggacc ttatacagat gatcttcagg aaatgatagc
     5461 ggagcttaat ctagatgaac acgtccagtt gacgggaatg attgcaccaa gtgatacagc
     5521 actttattat aaagctgcgg atttctttat ttctgcttcg accagtgaaa cacagggact
     5581 aactttctta gaaagtttag cttctggaac tcctattttg gcacatagca atccctactt
     5641 aaaaaatgtt gtaacagata agatgtttgg gacactcttc gttcgagaac aggagttggc
     5701 agatgccgtc gttgaggcga ttgttgctac accaccaatg aatccgtatg tgcttgataa
     5761 gaagctctat gagatttctt ctacgaattt tgggcgcaag gtttttgaat attatctaga
     5821 tttgaagatt tcgtatgatt ttagaaaaga acatacgaat caagatagcg tagcagaggc
     5881 attgataaaa gaggctattt acctaccgca aaaagtagta gttaaatcaa cagacacgac
     5941 agcaaaaata ttgaaaaaat cagtcgaaca agtaaaatca atccggaatt tctttgaata
     6001 atttggaggt ttcaaatgga aaagcttcgg aaagagagtt ggaagaatct ggttttggca
     6061 actcttatta ttctgtccat gtatgcaggt atttatctga ttgcctttca tctgattagt
     6121 gatactatgc tttctaagga actggtggtt gggaatatat tgctctatac tcccattgta
     6181 ctattcctaa caggtcttct ttatggattt tttcacggtt tttctcctag ccttcttgcc
     6241 ttggtgttac tgctttatcc tgtaaccatc tttttcttta aggaatggat tattctctat
     6301 cagattgtct acaccttatg tgctctgatt ggaaatgggg taggagctct gctattttct
     6361 ctaagaaaga aaatgaggaa aaatccttga tatatcggaa aatccttgtt ataatagctt
     6421 ttagagactt atcaattcta ggaaatcttt gagagagtgt gcggtgggtg caagcacata
     6481 gaggatagga ttgacactac tcagtatgat tttatgcaaa cataaaaggg tggttccctt
     6541 atcaccaatc tgaggtagtc aggcttttta gcgtgactgc gaatgaaggt ggaaccacgt
     6601 tacgacgtcc ttttgtgagg gcgtcgtatt ttcttttttg acaaaacaag gagatgaagt
     6661 tgaccagcat tccacaagaa acgattgatc gttgcctagc cgttcgcaag gcttacagag
     6721 agttggaact caaacaccac gacaaagagt ggtccattga ggagtgaaac agtctgggga
     6781 tagactgttt cagctcaaca acttaaaata aagacttgtt gaccaatgat attggcaaca
     6841 tgaaccgtct gattatgacc aagcagggtc gttactatga tgaaacgcct tataccttgg
     6901 agcataaaat ggctgaaaat atctggtggt tgattgaact ggctgaccgc ctggatattg
     6961 atattcaaaa agaaatggaa acctttttaa ctcagaaaga agagttattg gggattaaaa
     7021 aataaataaa aaggagataa acatgattaa aatcactttc ccagatggtg ctgttcgtga
     7081 gtaccaggct ggcgtgacaa cttttgaaat tgcggagagt atcagcaagt ctttggcgaa
     7141 aaaggcgctt gctggtaagt tcaatggtaa attgattgac accactcgtg ccattgaaga
     7201 ggatggcacg cttgaaatcg taacacctga ccatgaggat gccttggaca tcttgcgtca
     7261 ctcagcagct cacttgtttg cccaagcggc tcgtcgcctt ttcccagaca tcaagttggg
     7321 ggttggtcct gctattcagg atggtttcta ctatgacacg gacaatgcgg ctggtcagat
     7381 ttcaaatgaa gacctgccac gcattcagga agaaatgatg aagattgtca aggaaaactt
     7441 cccttcagaa cgtcgtgagg tgaccaagga agaagctatt gagattttca aaaacgatcc
     7501 ttacaagttg gaattgattc aagaacactc tgatgacgag ggcggtttga ccatttatac
     7561 ccagggcgaa tacattgacc tctgccgtgg cccacacgtt ccgtcaacag gtcgtatcca
     7621 gatttttgaa ttgctcaatg tggctggtgc ttactggcgt ggtaagagcg aaaatccaat
     7681 gatgcaacgg gtttatggta cagcttggtt tgacaagaaa gaccttaagg cttaccttca
     7741 aatgcgtgaa gaggcaaaag aacgtgacca ccgtaaactt ggtaaggaat tggatctctt
     7801 catgatcagc caagaagttg gtcaaggctt gccattctgg ttgccaaatg gagcgaccat
     7861 ccgtcgtacc ttagagcgtt acatcacaga taaagagttg acttcaggtt accagcacgt
     7921 ttatacgcca ccattggctt ctgttgaact ctataagact tcgggtcact gggagcatta
     7981 ttcagaagat atgttcccaa ctatggacat gggcgacggt gaagagtttg ttcttcgtcc
     8041 gatgaactgt ccgcaccaca ttcaagttta taaaaatcat gtgcgttcat accgtgaatt
     8101 gccagtgcgt atcgccgagc ttggtatgat gcaccgctat gaaaaatctg gtgcattgac
     8161 tggtttgcaa cgggtgcgtg agatgaccct caacgacggt catatctttg tcacacctga
     8221 gcaaatccaa gaagagttca aaaaagctct ccagttgatc atcgatgtgt acgcagattt
     8281 caacctaaat gactaccgtt tccgcctgtc ttatcgtgac ccagaggaca aagagaagta
     8341 ctatgacaac gatgagatgt gggaaaatgc ccaacgcatg ttgaaaggtg ccatggatga
     8401 aatgggcgta gactactttg aggcagaggg tgaagctgcc ttctacggtc cgaaattgga
     8461 tattcaagtg aagactgccc ttggtaacga agaaaccctg tcaactattc agttagactt
     8521 cctcttgcca gaacgctttg gtttgactta tatcggtgct gatggtgagg agcaccgccc
     8581 agtcatgatt caccgtggtg ttatctcaac catggaacgc ttcacggcta tcttgattga
     8641 aacctacaag ggtgccttcc caacttggtt ggctccaacc caagtgacta tgattccgat
     8701 ctctgtggaa gctcacttgg actacgcttg gaaggttgct aaggaattgc aagaccgtgg
     8761 cgttcgcgta cacgtggatg agcggaacga gaagatgcaa tacaagatcc gtcagagcca
     8821 gaccagcaaa attccatacc agctcatcgt cggtgacaag gaaatggaag acaatgctgt
     8881 caacgtccgt cgctacggaa gtaaggcaac gcaaactcag tctgtcgcag aatttgtgga
     8941 ccatatccta gcagatattg cccgcaaatc acgtccagct gaagctgaat aaataaagta
     9001 agaagtccga aaaggcttct tttttgacag caaaaactcc caaccaactg gctgggagaa
     9061 attttattct atactgctcg cccaagactt agccacataa cgtgacaggc ttgaaatggc
     9121 aaagttgatg ctaaagtaaa tcaaggctac catgagatag agtgcaaaaa cttgttctgc
     9181 ctcaaagtag cgtcccatga ggatttgact cgaaccgaag agttcttgca gggcgatgac
     9241 ggagtagagg aaactagtgt ccttgatgac cgttacaatc tgcgagatga tggctggcag
     9301 catcttgcga atggcctgcg gcatgatgat atagaccatg atttgcactg gggtaaaacc
     9361 ctgtgacaag ccagcttcgg tctggcccgg tccgatggcg tttagaccac cacggataat
     9421 ctcggccatg gctgccgtgg taaaaatagt aaaagcagta ataccagcag gcgttgactt
     9481 catttggaag actaagaaga cggtgaaaat ccagagcaag tttggtacat ttcgcacaaa
     9541 ctcgatatag atagttgcaa tccacttgaa aattggattt tttccattgc gcatgatggc
     9601 gagaatcatt ccgaaaagaa tggacaggat taccgaaatg cttgagatat agagtgttaa
     9661 cttcaaacca ttccacaaga gttggaaggt tgaaggtttg agtaattcta aaacataatt
     9721 catcgttcaa cctcctttta atgtgaaaag gcagacttat tcttttcttc aatgcgacgt
     9781 ccccagcttg caacagggaa gcacatgatg aagtaaagga aagcagcacc agcaaaggct
     9841 gggatgtagt tggcattcat ggcagaccaa gattttgtca taaacataat atcagctcca
     9901 gagataatgg caacagtagc cgtattctta attaagttta ctacttggtt ggtcaaaggt
     9961 ggaagaatgg tcggaatggc ctgtggcaag atgatgattc gcatggtttc agactgagta
    10021 aatccttgtg agagtgcggc ttcagtttgc ccagtaagca cagcctcaat ccctgcacga
    10081 atgacctctg cgatataggc accgtgatag agtccgacgc agataacagc tgtccagtag
    10141 atggacggca tgagaacata atttgaaatc agcggaagtc cataataaac aatcatgaac
    10201 tgaaccaaaa gcggcgtgtt ctgatagtat tcaacataga cacgcgcgat gatttttaaa
    10261 actttatttt tacttgaaga gagactacca aagacaattc ctaaaaacat ggccatgata
    10321 aaggcaccga cagaaatggc taaggtaaag agaaaacctt ggaaaaaggt tggaaaatct
    10381 ttgaaataac tgacccaatt ctcccaagcg taaggactag ttgttaaaac tgtcatattg
    10441 tttcaccttt ctattcgtca gtttctgtga ctggtttgag accattggca tcatagatgc
    10501 tttgtaggct accatcggca gtccattttt ctacgagact atttaagtag ttgttaaggt
    10561 ctttgttaga taatttagta acaattccat agtcagaagc tgagaagcta aagtccatga
    10621 gttcagattt agagctgata tacccagaaa ggatagattg gtctactgaa aatgcgtctg
    10681 tacggtgagc acgtagcgag acagagagtt ctggataaga accaagctct gcaaaattga
    10741 cagagatacc atatttgtct gctagtttag agatgagggt acgtgtaatg gaaccctgag
    10801 ctacaccgat tgtctttcca tccaggtcgg taaatgtctt aattccacta tccttattga
    10861 ccaagaagcc aacggcatct gtgtagtaag gagttgtgaa attgtagagg agtttacgtt
    10921 cctcagtgat agtgaaggtt gcgatgacca tatccacttg accgttatct aagagtggtc
    10981 ctcgggtttg cgctgtaacg ggagtaaatt cgatatctac acctaattca tctgcaattt
    11041 tacgagcaat atcaatctcc attccctcaa attcaccagt atcagggttc ttgtagccaa
    11101 agtttgggac gtcctgtttc actccgactc ggagaacgcc tctatcgatg atagcttgaa
    11161 cttgatcact attggtaatg gatgcgggtg tttctgtttg agctactggt tgttttgcga
    11221 aaacgaaaag aataagtcca aagaccaata atctaattgc aaagaaaatg ttgatttttt
    11281 tcttatccat agacttcctc ctagagtttt actttgtcag attcgtggtt gataatcttg
    11341 ctgaggaatt gttgggcacg aggctctgtc ggattgtcaa agaagccatt tacatctgtt
    11401 gtatcaacta aaacttctcc ctcagccatg aagatgatgc gatcagctac ttcacgggca
    11461 aagcccattt cgtgtgttac gacaatcatg ttcatcccgt cacgtgccaa tttttgcata
    11521 actgcaagta catcgccgat agtttctggg tccaaagcag atgttggttc atcgaagagt
    11581 agaagctcag gtttcatagc taagccgcga gcgatggcga cacgttgttt ttggcctccg
    11641 gatagcatag cagggtagga atccttccga tcccagaggt taacaaactc gagatacttt
    11701 tgagcagttt tttcagccgt agctttatcg attcccaaaa ccttaatcgg tgccaaagta
    11761 acattttcta acacggtttt gtgtgggtaa aggttgaaat gctggaaaac catgccaact
    11821 tctttgcgaa gtgatacgag ctctttgata gaagtagagg aaatatcatg tccattgacg
    11881 atgagcgttc cttcatcaat tgcctctaaa ccgttaatcg tgcgaatgag agtagatttt
    11941 ccggatccag aaggtccaag gagaacgaca acctgtccag gttcgaattc caagttgata
    12001 ttgcgtaggg cgtggtagtc gccatagtat ttgttgacat ctttaaattc tactaaagcc
    12061 ataaaattct ccttgcatag tgttttttag gtattcccat cttagcattg cctatataca
    12121 atgtcaagta aa
//
LOCUS       DAZQCJ010000058        12123 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000058 DAZQCJ010000000
VERSION     DAZQCJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12123
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.059"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            13..1320
                     /locus_tag="ACHU66_001391"
     CDS             13..1320
                     /locus_tag="ACHU66_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HFR3389668.1"
                     /translation="MFKKKATQIVSETSIKKSRKFLNRKTAGITGGILLTTFIGSQLM
                     TAELPKKDDLYGQQYVTVVKHLQEAGFKNIQGVELSDLEFGKIGESNLVELVSVDGED
                     WKEGRALKNIPITISYHVPKKDAVEFNLPASKNLADVEKELKDSGFKQVELTPVLLVE
                     EGNADKKDKIDRLQIGNHTYQSNHFYSTSLPVTLTYFDVSKDNIKLPENLAEAKTKPE
                     LEKQLKTAGFTDIKWTAVADKDKAKHEKIQKINLGGAEIQLPTKQEIISKKSTPIVIT
                     YYDFSSFAELPSSISTKTATDTKKLFTDGGFSQVSEVATETNEIAKNGQIIAVEIDGK
                     SFNEMNDTVIKKDSKVIIKYWNAEKAIAEKARKEEEERLAAEAQKVAEAQSQVQQFAA
                     TPSQNTYYPNCKAVRQAGAAPIYRGEPGYGSHLDRDGDGVGCE"
     gene            1382..1606
                     /locus_tag="ACHU66_001392"
     CDS             1382..1606
                     /locus_tag="ACHU66_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389669.1"
                     /translation="MAYKYRMILSFLLAGLCLYLVATVFAKSIWEGPLFLAFSFYSLI
                     YGCVMLYKWKPTAAKIIFQCIGEFLSLPWS"
     gene            1705..2286
                     /locus_tag="ACHU66_001393"
     CDS             1705..2286
                     /locus_tag="ACHU66_001393"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR3389670.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     AFGVVSSRIGMRRDAVAIDDDMDIFGFIEKMEPRPYCVLIDEAQFLRRHHVYALARVV
                     DELDVPVMAFGLKNDFRNELFEGSQHLLLLADKLDEIKTICQYCSKKATMVLRTQDGK
                     PTYEGEQIQIGGNETYIPVCRKHYFSPEIKELP"
     gene            2567..3646
                     /gene="prfA"
                     /locus_tag="ACHU66_001394"
     CDS             2567..3646
                     /gene="prfA"
                     /locus_tag="ACHU66_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR3389671.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEAATR
                     DTVTAYREYKTVLQNMVDAEEMIKESSGDADLEEMAKEELKQAKADKEAYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEGQGWRFEVMEASYNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLIMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVVDALVLYDQTQKLEELNK"
     gene            3646..4479
                     /gene="prmC"
                     /locus_tag="ACHU66_001395"
     CDS             3646..4479
                     /gene="prmC"
                     /locus_tag="ACHU66_001395"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR3389672.1"
                     /translation="MNYAQLFAAYEEQLVAIGEEAESLSFTFRGLKGLNFTEFLLLLR
                     QEVTPTDKEEIDAIFQQLSQHRPAQYIIGKADFHGLEFAVDERVLIPRPETEELVNLI
                     LQENSGAGLRILDIGTGSGAIAISLAKARSDWEVVAVDISKDALAVAQENARTNQVSV
                     HFLESDVFQAVTGQFDLIVSNPPYISPDDTDEVGLNVLASEPHLALFAEEDGMAIYRQ
                     IAEQAGAFLKEKGKLYFEIGYKQGQDLTDLLALHFPKKRIRVLKDQFGQDRKVIADDN
                     G"
     gene            4466..5074
                     /locus_tag="ACHU66_001396"
     CDS             4466..5074
                     /locus_tag="ACHU66_001396"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR3389673.1"
                     /translation="MTMDKLRTILENGGAVILPTETVYGLFAQALNEDAVNRVYQLKQ
                     RPRDKAMNLNVSNLNDIYFFSQNTPFFLEKLYNRFMPGPLTIILKANENVPFWVNSGL
                     DTVGFRLPNHEQTLRLISETGPLIGPSANISGNESGKKFSDIQAQFSVDLPGIEDDQA
                     LTGIDSTILDLSGQKARILRQGAISREEIQKAIPEIEFEVVL"
     gene            5071..5502
                     /locus_tag="ACHU66_001397"
     CDS             5071..5502
                     /locus_tag="ACHU66_001397"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR3389674.1"
                     /translation="MIRPIEKGDLVVIREINAQSLGYDCSLEQTERQFFRCTSTLGHI
                     LLVYIDDISGAVQGYIHAQVYESLYSDTGLNILGLAVLPGHQVQGIGASLLKAVEQIA
                     QKEGYHFIRLNSAESRLQAHLFYEKNGYHSDKMQKRFIKHI"
     gene            5526..6785
                     /gene="glyA"
                     /locus_tag="ACHU66_001398"
     CDS             5526..6785
                     /gene="glyA"
                     /locus_tag="ACHU66_001398"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR3389675.1"
                     /translation="MIFDKVNYKEFDKEVWEAIQAEEKRQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTECVDVVESLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMALIEPGDTVMGMDLAAGGHLTHGASVSFSGQTYNFVAYNVDEETGLLDYDAIL
                     KQAKEVQPKLIVAGASAYARTIDFAKFREIADAVGAKLMVDMAHIAGLVAAGLHPNPV
                     PHAHITTTTTHKTLRGPRGGLILTNDEELIKKINSAIFPGIQGGPLEHVIAAKAVSFK
                     EVLDPDFKDYAQKVIENSKAMAEVFLANPNFKVITGGTDNHLFLVDVTKVVENGKVAQ
                     HLLDEVNITLNKNSIPYEKLSPFKTSGIRIGSAAITARGFGVEEARKVAQLTIKALEN
                     AENEKALEEVRQEVRALTDQFPLYEGL"
     gene            6785..7759
                     /locus_tag="ACHU66_001399"
     CDS             6785..7759
                     /locus_tag="ACHU66_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFR3389676.1"
                     /translation="MDIFVKKAIIHQFSPDDTELVLSDQLLTVSPKIEEYLRKKIERV
                     FSDEAKTGQFNPDNPFLDYLDGDLLTNSVKIANLWKEAFSISENLKTNDLIFIEFERN
                     GVEHFTFLRISLRENLAHVGLESGSPLKITQNNLPGAGSAPDEALIVNLQTRKYHLIE
                     KRIKHNGSFLNYFSDNLLQVTPAISAKKSIKAVEQTAQKIADNFHQGDFQFQSKIKSA
                     IFNNLEEDNELSPEKLADQLFDNNLTARLNFVDQLKEVIPDKISFDEIDSSRQLKKFE
                     NQKLSLSNGIELIVPNAIYEDAESVEFIQNDNGTYSILIKNIEDIKSK"
     gene            7760..8359
                     /locus_tag="ACHU66_001400"
     CDS             7760..8359
                     /locus_tag="ACHU66_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFR3389677.1"
                     /translation="MKKLIRTLILLLLIFLGYKFYQTYSAVKQVMTYQTMVQEVLAEN
                     DTPANEELVLAMIYTETKGQEADVMQSSESATGYANTITDSRESIRQGIIYLTENLRL
                     AEEKGVEVWTAVQAYNFGPAYIDYIAEHGGEHTLSLAKEYSRTVVAPSLGNTTEETYT
                     YFHPLALLNGGKLYVNGGNIYYARQVRFNMHLMRLINLF"
     gene            8460..9218
                     /locus_tag="ACHU66_001401"
     CDS             8460..9218
                     /locus_tag="ACHU66_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFR3389678.1"
                     /translation="MKRNVLITGASSGIGKAIAYVFAANGDNIIITGRRLDKLEEIKQ
                     DIEKQYHVQVSVHSFDITNMEQTIVNCQKILEEVGQIHILVNNAGLALGLDKFQDYDL
                     TDMMTMIDTNIKGLLTVTRQLLPQMVENNEGHIINIGSTAGIYAYAGAAVYAATKSAV
                     KVLSDGIRIDTIDKNIKVTTLQPGIVETDFSQVRFHGDKERAATVYQGIDALQANDIA
                     SCTLFVANQPAHVQISDITIMATKQATGFTIHRD"
     gene            9235..9987
                     /locus_tag="ACHU66_001402"
     CDS             9235..9987
                     /locus_tag="ACHU66_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6287 domain-containing protein"
                     /protein_id="HFR3389679.1"
                     /translation="MKRKYLALALGLLTIMSLSACQTEKKGSSASTSTADTQVSNANT
                     STSSTIQREISLSSMDIDALMQGNYDSILGTWQNSQGNSLVFNSKGLVADSQSLLGRG
                     KITDGIFETGYVDATIGDVTLLMIPKGTQANTTETDTTDSTRDRMIVISQETTADVVD
                     VYYRLTNSATSGIDPLKNTETGVQLDSGPKTIDYANSILGENNWRVIEGNYTRTESIP
                     YNILEGDDNARYTIYQNGVIINADYQIVYQPQ"
     gene            9984..10316
                     /locus_tag="ACHU66_001403"
     CDS             9984..10316
                     /locus_tag="ACHU66_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HFR3389680.1"
                     /translation="MILNVQDFHLAAFAYYDGLDVIDQLKPGTPVQLVGEPSNPHDSE
                     AVAIFYQGTKLGYIPSDKNSLISRLIYFGHGDILEARIQMSNTENHPDRQFRVVVKLK
                     DNRKNDSR"
     gene            complement(10337..11284)
                     /locus_tag="ACHU66_001404"
     CDS             complement(10337..11284)
                     /locus_tag="ACHU66_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389681.1"
                     /translation="MDVFPSFFIFLFNKLNIFVDLKNIFCYTCVMKTSTLGKLLELPN
                     FHHFYTVPIRENTESSGNYIIVFEQDKQPVFVDLKNETGHTREVRFASHFDSAGLLVG
                     LGAIPINYWPTCQILGVQKNNNRRGLSLSSLMDGYGRLPIFKEMAERYRARGQSTIYD
                     PYRYSYTIDEKKVIKDNQLTLIPKKEYEKIKDFHPFLQTKHGNVYAVPCFYQDRKRNQ
                     TIKIYIIGWYFYEKQLKISWKPSHSRKSRWRSSLVSVDKLSDRFFFQGELYKKGTYPV
                     FLEDLTIKNKAYQHRLDREFAAFLSIIEEQKRRAQLRVK"
     gene            complement(11312..12100)
                     /locus_tag="ACHU66_001405"
     CDS             complement(11312..12100)
                     /locus_tag="ACHU66_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389682.1"
                     /translation="MAVLKYSKARLLVLLIATGLSCIGIYWLGKEQNRLLNEQCHALN
                     IRIINDLGAKIDAIGGPQNPRIIGFFQQDDTTAISQRIGTASEEELKIAKPDNLFQKE
                     WIVLYPQTRSSPFENTSAYAVMKTSIKADWLHVTTSSETELDIFYEKADESLLTLEDL
                     VQDKESFHATLKTILVSAKNEAEIQVQKDILEMFESDDWSAIPFAYTEKSLILEKAVI
                     SVSAFVDSLNPYYFSEQTLADLRLSEESRQALEDSVDKTIITYP"
BASE COUNT         3933 a         2374 c         2458 g         3358 t
ORIGIN      
        1 ggagaatgat ttatgtttaa gaaaaaagca actcagatag ttagcgaaac gtcaattaaa
       61 aaatctagaa agtttcttaa tcggaaaaca gcaggaataa ctggtggcat cctacttaca
      121 acatttatcg gttcacaatt aatgactgct gagcttccaa aaaaagatga cctttatgga
      181 caacagtatg taacagtcgt aaaacatctt caggaagcag gttttaaaaa tattcagggg
      241 gtggaactta gtgatttaga atttggaaaa ataggtgaaa gcaatcttgt cgaactcgtc
      301 tctgttgacg gagaagattg gaaggaagga cgtgctttaa aaaacatccc aattacaatc
      361 tcctaccatg ttcccaaaaa agatgccgtt gaattcaacc taccagcttc taaaaattta
      421 gcagatgtcg aaaaagagct gaaagattca ggttttaagc aagtcgaact aactcctgtt
      481 ctgctagtag aagaaggcaa tgcggacaaa aaagataaaa ttgatcgcct tcaaatcgga
      541 aatcacacct atcaatccaa tcatttttac tctactagtc tccctgttac attaacctat
      601 tttgacgtta gcaaagataa catcaaacta ccagaaaatc tagcagaggc taaaacaaaa
      661 ccagagctcg aaaaacaact aaaaacagca ggatttacag acatcaagtg gactgctgtt
      721 gcagacaagg ataaagcaaa gcacgaaaaa attcaaaaaa ttaatctagg tggtgcagag
      781 attcaattgc ccaccaaaca ggagattatc agcaaaaaat caaccccaat tgtgattacc
      841 tactacgatt tctctagctt cgctgaactg ccatcatcca tttcaacgaa aacagctaca
      901 gatactaaaa aattgtttac cgatggtggt ttttctcaag tatctgaagt agcaacagag
      961 accaatgaaa ttgctaaaaa cggacaaatc attgcggtcg agattgatgg aaaatctttt
     1021 aacgagatga acgatacggt catcaaaaaa gatagcaaag tcattatcaa atattggaat
     1081 gctgaaaaag ctattgctga gaaagcacgt aaagaggagg aagaacgcct cgctgccgaa
     1141 gcgcaaaaag tagcagaagc acaaagtcaa gttcagcaat ttgctgctac accatctcaa
     1201 aacacatatt atccaaattg taaggcagtt agacaagcag gagctgcacc aatctatcgt
     1261 ggagaaccag gttacggtag tcatttagac cgtgatggag atggagttgg atgcgagtaa
     1321 gaaaaaacat tgacaacgtt tacattgcat gctatactgc tcttataagg agggattact
     1381 catggcctat aaataccgta tgattttatc gttcctactc gctggattat gtttgtatct
     1441 agtagcaact gtttttgcca agagtatctg ggaaggaccg ttgtttcttg cattttcttt
     1501 ttacagcctg atatatggtt gtgttatgct ctacaagtgg aaaccaacgg cagctaaaat
     1561 cattttccaa tgtattggag aatttctctc tttaccatgg tcttaatatc aaacaagctc
     1621 aaaacgggct tgtttttcta tttcctaaat ggtcaattta tgatagaatg gaaggagtga
     1681 aaatttaaga ttggaaagag aaaagtggct caattatatt ataaatatgg gacaatgaac
     1741 tcaggaaaaa ccattgagat tttgaaggtt gcccacaact atgaagaaca aggaaaacct
     1801 gttgttatca tgacatctgc acttgatact cgtgatgcct ttggtgttgt atccagtcgt
     1861 attggcatga gaagagatgc agtggcgatt gacgatgata tggatatctt tggttttatt
     1921 gaaaaaatgg aaccgcgtcc ttactgtgtt ctcattgatg aagcacaatt tcttcgacgt
     1981 catcatgtct acgcacttgc tcgcgtagtc gatgaattag atgtacccgt tatggctttc
     2041 ggtcttaaaa atgatttccg aaatgaacta tttgaaggtt cccaacattt gctcttattg
     2101 gctgataaat tagatgaaat caaaacaatc tgccaatatt gttctaaaaa agcgacaatg
     2161 gttttgagaa cacaggatgg aaaacctact tatgaaggag aacaaatcca aattggtggc
     2221 aatgaaacct acattcctgt ctgtcgcaaa cattattttt caccagaaat taaagaatta
     2281 ccctaatttt tgaaaatgaa atgagaagcg actgtaaatt gagcaactgt atagaactga
     2341 atttgcctat gactctgtgc caattttcat gacttacata ctacggcaaa ggaattgaac
     2401 acgccctaca agctaactag aagtgtgttc tgtcaaatat ttagaaagga attgaatacg
     2461 ggctacgaac tgtgcaaaaa agatagtttt tcctaggacg ctttcgtcct tcgtcaaaac
     2521 tcctattttt gctttgttcg tttaacgccc ttaatatctt aaaattatga acatctacga
     2581 tcaattacag gcggtggagg accgctacga ggaacttggt gaattgttgt ctgaccctga
     2641 tgtagtcagc gataccaagc gttttatgga gttgtctaag gaagaagctg caactcgtga
     2701 tacagtaaca gcctaccgcg aatataagac tgtacttcaa aacatggtcg atgcagaaga
     2761 gatgattaaa gaatcctctg gcgatgctga tttggaggaa atggctaagg aagaactcaa
     2821 acaagctaag gctgataaag aggcttacga agaaaagctg aaaatccttc ttttaccaaa
     2881 agaccctaac gatgacaaaa acattatctt ggaaatccgt ggtgccgcag gtggtgatga
     2941 agccgccctc tttgctggag accttttgac catgtatcaa aagtatgcag aaggtcaagg
     3001 ctggcgtttt gaagtcatgg aggcttctta taatggtgtc ggcggtatca aggaagtggt
     3061 tgccatggtc tctggtcaat ccgtttattc aaaactcaag tatgaatcag gagctcaccg
     3121 tgtccaacgt gtccctgtga cagaaagcca aggtcgtgtc cacacctcaa cagcaactgt
     3181 cctcatcatg ccagaagttg aagaagtgga atatgacatc gatccaaaag atcttcgtgt
     3241 cgatatttac cacgcttctg gtgctggtgg acagaacgtc aacaaggttg caactgccgt
     3301 gcgtatcgtt cacttgccaa ccaacatcaa ggtagaaatg caggaagaac ggacccagca
     3361 gaaaaaccgt gaaaaggcta tgaagattat ccgtgcccgt gtagctgacc actttgcaca
     3421 gattgcccaa gatgagcagg atgcagagcg taaatcaact atcggtacag gtgaccgctc
     3481 agagcgtatc cgtacttaca atttcccaca aaatcgtgtg acagaccacc gtatcggctt
     3541 aaccttgcaa aagctcgata ccatcttgtc aggtaagatg gacgaagtcg tagatgccct
     3601 tgttctctac gatcaaacgc aaaaactaga agagcttaac aaataatgaa ttacgctcaa
     3661 ttatttgcag catacgaaga acaattagtc gcaatcggtg aagaggcaga atccctgtcc
     3721 ttcacctttc gtggcttaaa gggattgaac tttacggaat ttctcctcct gctccgtcag
     3781 gaagttacac cgactgacaa agaagagatt gatgcgattt ttcagcaact ttcccaacac
     3841 cgacctgccc agtacattat cggcaaggct gattttcatg gcttggagtt tgcggtggat
     3901 gagcgagttt tgattccaag acctgaaacg gaagaattag ttaatttgat tttgcaggaa
     3961 aatagcggag cgggtttgcg tatcttagac atcggtacag gaagcggtgc catagctatt
     4021 tcccttgcaa aagctagatc tgattgggaa gtggtagcag ttgacatttc aaaggatgcc
     4081 ctagcagtgg ctcaggaaaa tgcaagaacc aaccaagtct ctgttcactt tctagaatcg
     4141 gatgtcttcc aagcagtgac agggcaattt gacctcattg tttctaatcc gccctatatc
     4201 tcaccagatg acacagacga agttggactc aatgtattgg cttctgagcc tcatctggcc
     4261 ttatttgcgg aagaagatgg catggctatc tatcgacaaa ttgcggagca agcaggggca
     4321 tttttgaagg aaaaaggaaa actctacttt gaaatcggct acaagcaagg gcaagaccta
     4381 actgacttac tcgctctgca tttccccaaa aagcgaatta gagtcctcaa agaccaattt
     4441 ggacaggata gaaaggtcat agcggatgac aatggataaa ctccgaacaa ttcttgaaaa
     4501 tggtggtgct gttatccttc caactgaaac agtctatggg ctttttgccc aagccttgaa
     4561 tgaagatgca gtcaatcgtg tctatcagtt gaaacagcgt ccgagggaca aggcaatgaa
     4621 cttaaatgtc tcaaatctga acgatattta ttttttcagt caaaatacac cattttttct
     4681 ggagaaattg tataatagat ttatgccagg accactaacg attatcctga aagcgaacga
     4741 aaacgtccct ttttgggtta attctggact ggatactgtt ggatttcgtc ttcccaatca
     4801 cgaacaaacc ttacgtttga tttcagagac tggcccactt attggaccgt ccgccaatat
     4861 ttcaggaaac gaaagtggca agaagttttc ggacatccag gcacaatttt cagttgattt
     4921 gcctggcatt gaggatgacc aagctctgac aggcattgat tcaacgattt tggacctttc
     4981 tggacagaaa gcgcggattt tgcgccaagg tgctatcagc cgtgaagaga ttcagaaagc
     5041 cattcccgaa atagaatttg aggtagtttt atgattcgtc ccattgagaa aggtgacttg
     5101 gtagtcatcc gagaaatcaa tgctcaatcc ttagggtatg attgttcatt ggaacaaact
     5161 gagcgacaat tttttagatg tacgagtaca ttggggcata tcttgttggt ctatatagat
     5221 gacataagtg gcgcagtgca aggttacatt cacgcacaag tctatgaaag tctctattcg
     5281 gatacaggcc tcaatattct cggactagct gttttgcctg gtcatcaagt acaagggatt
     5341 ggagcaagcc tgctgaaagc tgttgagcaa attgcacaga aagagggcta ccattttatt
     5401 cgcctcaatt ctgctgaaag tcgtctccaa gcccatcttt tctacgaaaa gaatggctat
     5461 cactccgata aaatgcagaa gcgttttatc aagcacattt agcccaatac aaaggagtac
     5521 cgtatatgat ttttgacaaa gttaactaca aagaatttga taaagaagtt tgggaagcta
     5581 tccaagctga agaaaaacgc caacaaaata atatcgaatt gattgcgtct gaaaacgttg
     5641 tttctaaagc tgttatggca gctcaaggtt ctattttgac caataaatac gcagaaggtt
     5701 acccaggtcg tcgttactat ggcggtacgg aatgcgttga cgtagttgaa agtttagcta
     5761 ttgaacgtgc taaagaaata ttcggtgcga aatttgctaa cgttcagcca cactcaggaa
     5821 gccaggccaa ttgtgctgcc tacatggcct tgattgagcc aggagacact gttatgggga
     5881 tggacttagc tgcaggtggt cacttgactc acggtgcatc agttagcttc tcaggacaaa
     5941 cttacaactt tgtagcttac aatgttgacg aagaaactgg cttgcttgat tacgatgcaa
     6001 ttttaaaaca agcaaaagaa gtacagccaa aattgattgt agcaggtgct tcagcctatg
     6061 cacgtacaat tgactttgct aaattccgcg aaattgcaga tgcagtgggt gccaaactga
     6121 tggtggatat ggctcatatt gctggtcttg tagcagcagg tcttcatccg aacccagtgc
     6181 cacatgccca tattacaaca actacgactc ataaaactct tcgtgggcca cgtggtggtt
     6241 taattttgac caatgatgaa gagcttatca agaaaatcaa ctcagccatc ttcccaggca
     6301 tccaaggtgg tccattagag catgttattg ctgcaaaagc cgtatctttc aaagaagttt
     6361 tggacccaga tttcaaagac tatgctcaaa aagtgattga aaacagcaag gccatggctg
     6421 aggtcttcct tgcaaaccct aactttaaag tcatcacagg aggaacagac aaccatctct
     6481 tcctcgttga cgtgaccaaa gttgttgaaa atggtaaagt tgctcagcat ctcttggatg
     6541 aagtaaatat tactctcaat aaaaactcaa ttccttacga aaaactctca ccattcaaaa
     6601 caagcggtat ccgtattggc tctgcggcca ttactgctcg cggttttgga gtagaggaag
     6661 ctcgcaaagt tgcacaacta actatcaaag cacttgaaaa tgcagaaaat gaaaaagctc
     6721 ttgaggaagt ccgtcaagaa gtgcgtgctt tgacagatca attcccactt tacgaaggtt
     6781 tataatggat atatttgtaa aaaaagccat catacatcaa tttagtcctg atgatacaga
     6841 gctggtcctt tccgaccagc ttttgactgt tagcccaaaa atagaagaat atttgcgtaa
     6901 aaagattgaa cgtgtctttt ctgatgaagc aaaaacagga caatttaacc ccgacaatcc
     6961 ttttcttgac tatttagatg gtgacctact gaccaactct gtcaagattg ctaatctatg
     7021 gaaagaagca ttttcaattt ctgagaattt aaaaaccaat gacctcattt tcatagagtt
     7081 tgaacgaaat ggtgtggaac attttacctt tttgagaatc tcactgcgtg aaaatctcgc
     7141 ccacgttggt ctggaaagcg gaagtccttt aaaaatcacc cagaataatt tacctggagc
     7201 aggttcagca cctgatgaag ccttgattgt caatcttcaa acgcggaagt atcatttgat
     7261 tgagaaaaga atcaaacaca atggatcctt cctcaattat ttctcagata atcttctgca
     7321 agttacacca gctatttctg caaaaaaatc aatcaaagca gtagagcaaa ctgcacagaa
     7381 gattgctgac aatttccatc aaggcgattt tcaatttcaa tcgaaaatca agtcagctat
     7441 tttcaacaat ttagaagaag ataatgagct ttcacccgaa aaattggctg atcagttatt
     7501 cgataacaac ctgaccgctc gtttaaattt cgtcgatcaa ctgaaagagg tcatacctga
     7561 taagatttcc ttcgatgaaa ttgatagtag ccgtcaactt aagaaatttg aaaatcagaa
     7621 gctatcttta tcaaacggaa ttgagttgat tgtgccaaat gcaatctatg aggatgcaga
     7681 atcggttgag tttattcaaa atgacaacgg aacctattct attcttatca aaaatattga
     7741 agacataaag agcaagtaaa tgaaaaaact gatacgtacg ctgatacttc ttttactgat
     7801 ttttctcggt tacaaattct atcaaaccta cagtgcagtc aagcaagtga tgacctatca
     7861 aactatggta caagaagtat tggcagaaaa cgacacacca gctaatgaag aactagtcct
     7921 agcaatgatt tacacagaaa ccaaaggaca agaagcagat gtcatgcaat caagtgagtc
     7981 cgctactggc tatgctaaca ctatcacgga tagtagagag agtatcaggc aaggaattat
     8041 ctacctaaca gaaaacctac ggttggctga ggaaaaagga gtagaggttt ggacagcagt
     8101 tcaggcatat aatttcggtc cagcctatat tgactatatt gctgagcatg gtggagaaca
     8161 tacactctct ttagctaaag aatactccag aacagtagta gcaccaagct tagggaatac
     8221 tacagaagaa acatatacct attttcaccc cttggctttg ctaaatggtg ggaagttata
     8281 tgtaaatggt ggaaatatct actatgctag acaggttcgt tttaatatgc atctgatgcg
     8341 cctcattaat ttattttaat acctatcaaa ctcatcggaa atctgatgag tttttaacta
     8401 cagcattttt acaaactagg taaaattgta gtataataaa gagaatagga gacagagtga
     8461 tgaaacgaaa tgtattgatt acaggtgcaa gtagtggtat tggcaaagca atcgcttatg
     8521 tgtttgctgc taatggtgat aatatcatca ttacaggacg aagattggac aaactagaag
     8581 aaattaagca agatattgaa aagcaatacc atgttcaggt atcagttcat tcatttgata
     8641 ttacgaatat ggaacaaaca attgtaaatt gtcaaaaaat cttagaagaa gttggacaaa
     8701 ttcatatttt ggtaaacaat gctggtctgg cattgggatt agacaaattc caagactatg
     8761 atttgactga catgatgaca atgattgata ccaatattaa aggattacta actgtgacac
     8821 gtcagttatt gccacaaatg gttgaaaaca atgagggtca tattatcaat attggctcaa
     8881 cggccggtat ttacgcctat gcaggagctg ctgtctatgc tgcaaccaaa tcagctgtca
     8941 aggtgctttc agacgggatc cgtattgaca cgattgataa aaatatcaag gtaactacgt
     9001 tacaacccgg tatcgttgag actgatttta gccaagtccg ttttcatgga gataaagaga
     9061 gagcagcgac agtctatcaa ggaattgatg ctcttcaagc aaatgatatt gccagctgta
     9121 ctctatttgt cgcaaatcaa cctgcacacg tgcagatttc tgatataacc attatggcaa
     9181 ccaaacaggc aactggcttt actattcata gagattagag gggagatagc tagaatgaaa
     9241 agaaaatatt tagcactcgc tcttggcttg ctcactatca tgtcgctttc tgcctgtcaa
     9301 actgaaaaga agggcagttc cgcaagtacc tcaactgctg atactcaggt ttctaatgcc
     9361 aatacaagca cgtcatcaac aatccaacgt gagatttctc ttagttctat ggatatagat
     9421 gctctaatgc aaggcaatta cgatagtata ctaggcacct ggcaaaatag ccaaggaaat
     9481 tcactagtct tcaatagtaa aggattagta gcagatagcc agtccttact tggccgcgga
     9541 aaaataactg acggaatttt tgaaacaggc tatgtagacg caacgattgg cgatgtaaca
     9601 ctattaatga ttcccaaagg aacacaagca aatacaactg agacagacac tactgactct
     9661 acgcgcgata gaatgattgt aataagccaa gaaacaacag cagacgtggt agatgtctat
     9721 tatcgcttga ccaattcagc cactagcggt atagacccat taaaaaatac tgaaacgggc
     9781 gtccagttgg atagtggacc aaaaacgatt gattatgcca actcaatttt aggcgaaaac
     9841 aattggcgcg tcattgaagg taactacacg cgcaccgaat ctattcctta taatattttg
     9901 gaaggcgatg ataatgcccg ttacactatt taccaaaacg gtgttattat caatgcagat
     9961 tatcaaattg tttatcaacc acaatgattc tcaatgttca agattttcat ttggcagcct
    10021 ttgcctacta tgatggacta gatgtaatcg accaactcaa gccaggaaca cctgttcagc
    10081 tagtcggaga gccttcaaac ccacatgact cagaagctgt agcaatcttt tatcagggca
    10141 caaagttagg atatatccca tcggataaaa actcactaat cagtcggtta atctattttg
    10201 gacatggcga tattctagaa gcacgtattc agatgtccaa tacagaaaat catcctgatc
    10261 gccagtttcg agtcgttgta aaactaaaag ataatcgtaa aaatgatagt cggtaattta
    10321 ccaacctacc cattttctac ttaacacgga gctgtgctct ccgtttttgt tcttcaataa
    10381 tacttaaaaa ggctgcaaac tccctatcca ggcgatgttg gtaggcctta ttttttattg
    10441 tgagatcttc caggaacact ggataggttc cttttttata caattctccc tgaaagaaga
    10501 agcggtccga taatttatca actgaaacaa gagaagaacg ccagcggctt ttacgagagt
    10561 gagaaggttt ccaagatatt tttaattgct tctcataaaa gtaccagcct ataatataga
    10621 ttttaatagt ctgatttcgt tttctgtctt gatagaaaca aggaactgca taaacatttc
    10681 catgctttgt ttgcaaaaaa ggatgaaaat cttttatctt ttcgtattct ttctttggaa
    10741 taagggttaa ttgattatcc ttaataacct tcttttcatc aatagtatat gaataacggt
    10801 aggggtcata aatggttgat tgtccacgcg ctctataacg ctctgccatt tccttaaaaa
    10861 taggtagacg tccataacca tccatcagac tagatagact aagtcctcgt cgattattat
    10921 ttttctgcac accaagaatc tggcaggtag gccaataatt gataggaatg gctcctaagc
    10981 cgaccagaag accggcgctg tcaaaatgac tggcaaagcg tacctcacga gtgtgaccag
    11041 tctcattttt caaatcaaca aatacaggtt gcttgtcctg ttcaaaaaca attatataat
    11101 ttccagaaga ctccgtattt tctctaattg gaacggtgta gaagtggtga aaatttggta
    11161 attctagtaa ctttccaagt gttgatgttt tcataacaca agtataacaa aaaatatttt
    11221 tcaaatccac aaaaatgttt aatttgttaa acaaaaatat aaagaagctg ggaaagacat
    11281 ccatgacgac tttcccactc ccaccgttta ttcaaggata ggtgataatt gtcttatcta
    11341 ccgaatcttc taaagcctgg cgcgattctt ctgataaacg caaatctgca agggtttgtt
    11401 ctgaaaaata gtagggattt agactatcta caaatgcaga aactgaaatg acagctttct
    11461 ccaatatcag agatttttca gtgtaagcaa agggaatagc agaccaatca tcactctcaa
    11521 acatttctaa aatatctttt tgaacttgaa tttcagcctc atttttagct gaaactagga
    11581 ttgtttttag tgtagcatga aaactttcct tgtcttgcac caagtcttcc aaggtcagca
    11641 agctttcatc tgccttctca taaaaaatat ctaattccgt ctctgacgaa gtagtcacat
    11701 ggagccagtc agccttgata ctggttttca ttactgcata ggcagaagta ttttcaaagg
    11761 gactgctgcg cgtttgagga tagagaacaa tccactcttt ctggaataga ttgtcaggtt
    11821 tagctatttt cagttcctcc tcagaagcgg taccaatccg ctggctgata gcagtagtgt
    11881 catcttgctg aaagaatcca atgatacgcg gattttgcgg accgccaatg gcatcaattt
    11941 ttgctcccag atcattaata atgcgtatgt tcaaggcatg acactgctca tttaatagtc
    12001 tattttgctc ttttccgagc cagtagattc cgatacaaga tagaccagtg gcaataagca
    12061 agacaagtaa ccgcgctttc gagtatttca acacagccat cacacctttc taaaacgctt
    12121 tca
//
LOCUS       DAZQCJ010000059        11473 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000059 DAZQCJ010000000
VERSION     DAZQCJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11473
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.060"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..428
                     /locus_tag="ACHU66_001406"
     CDS             <1..428
                     /locus_tag="ACHU66_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR3389683.1"
                     /translation="SQAVEPSSWSKGWFAQDLINLDEEKTAFENVSCTSLQDRMTILN
                     LLGMLGLSYDKAQQKVANLSGGERVRLSLAKVLLADANLLILDEPTNFLDMAAIEALE
                     TFLKEYEGSVLLVSHDQQFVETSVHRQWEIVQACLVKKS"
     gene            520..2595
                     /gene="pbp2b"
                     /locus_tag="ACHU66_001407"
     CDS             520..2595
                     /gene="pbp2b"
                     /locus_tag="ACHU66_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2B"
                     /protein_id="HFR3389684.1"
                     /translation="MKKRKNKPLEHDGNFISLRLNILFSIVIFLFLVLILRLADMQII
                     NHDFYSNKLSTASQKIISNGSIRGQIYDAQGKPLVENEIQQVVSFTRPNKMSAQEMKE
                     VANKLLQWVNVSDVTITQRDKADYYLADEEVYRQVVEALPKDKKYDSDGNYLSESTIY
                     SNAVDSIPAEALQYSEQDNKAIELFKQMNGATYFSTVNLVTDTLTSEQVAHIVANESQ
                     LPGISTTNNWQRTILPTSLSSIIGTVTSEQAGLPAEDAEYYLSKGYSSNDRVGTAYLE
                     KQYEEVLQGQREKKQINLDRNGNIESIETIQEGQQGNNIKLTIDLAFQDGVNAILRKY
                     FESELATGSALYSEGVYAVALNPSTGAVLAMSGYSHEKGSSTITEDALGTITSVFTPG
                     SIVKGATISSGWENGVISGNQVQLDEPIYFAGSAPITSWYGAYGSFNIDATEALEYSS
                     NIYMVKIALGLLGQTYSANMYLNDGDTLKNAMTKLRSTFAEYGLGASTGIDLPLESTG
                     FLPDKYTTANFITNAFGQFDNYTPMQMAQYAATIANNGTRISPHLVEGIYGNNAQGGL
                     GELIETVSGKEMNQVNISAEDMSLLRQGFYQVVNGSGRFNTGSAIGRGAAVTISAKTG
                     TAETYTTTASGDVVTAINTNVVAYAPSDNPQIAVAVVLPNLTNQSSTTTKTITQEIIN
                     LYQSLHPMN"
     gene            2604..3200
                     /gene="recR"
                     /locus_tag="ACHU66_001408"
     CDS             2604..3200
                     /gene="recR"
                     /locus_tag="ACHU66_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR3389685.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMEDDVVNEFA
                     KNLLAAKRDLSYCSICGNLTDQDPCAICQDSTRDQSTILIVEDSRDVTALENIQEYHG
                     LYHVLHGLISPMNGIGPDDINLKTLLTRLMDNEVTEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            3305..4351
                     /locus_tag="ACHU66_001409"
     CDS             3305..4351
                     /locus_tag="ACHU66_001409"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR3389686.1"
                     /translation="MSKETLILLYGGRSAEREVSVLSAESVMRAINYDKFFVKTYFIS
                     QTGDFIKTQEFSQTPSADEKLMTNATIVEEQKIRPSDIYEEKAVVFPVLHGPMGEDGS
                     IQGFLEVLRMPYVGTNILSSSVAMDKITTKRVLESAGISQVPYVAVIEGENIDDKIAE
                     IEGKLTYPVFVKPANMGSSVGISKAEDLAGLHKALDLAFKYDSRVLVEQGVNAREIEV
                     GLLGNADVKTTLPGEVVKDVAFYDYDAKYIDNKITMEIPAKIDEAIMDTMRDNAAKAF
                     RAIGGCGLSRCDFFLTEDGDIFLNELNTMPGFTQWSMYPLLWDNMGLAYPDLIEELVR
                     LAKEMFEKRESHLI"
     gene            4439..4819
                     /locus_tag="ACHU66_001410"
     CDS             4439..4819
                     /locus_tag="ACHU66_001410"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR3389687.1"
                     /translation="MYQTIVKGEGLFQALSQGYGESIRTFGVTEKGETPVSLVNIGLA
                     ACITMCVQGYYASQEGNKTMPVQVESRLDNQRFEVLIGIGESVSAQKQKEILAYVEKK
                     CKVKALLREDLQFETSFYVLESVE"
     gene            4821..5897
                     /locus_tag="ACHU66_001411"
     CDS             4821..5897
                     /locus_tag="ACHU66_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR3389688.1"
                     /translation="MFLAIEEMRQNKLRYGLILGLLILISYLVFFLTGLAYGLMQENR
                     TAVDKWQADYVLLNSESNRLITASKIDTSLLDQVDASDKALIRQQAGVAYVDENATSD
                     EKEKVNIFAVESDSFIVPNIVEGRLYEKTGEVLVDKTLSEVEGFGLGDQIYLSGSDEK
                     VTIVGYTDNAYFGVAPVVYMDFTAFAELMQADKQQAATSNLASAIVVRGDAASLPNEL
                     EKVAIADFIENLPGYKAQNMTFGFMIGFLIVISAIVIGIFIFVLTTQKSPIFGLMKIQ
                     GLSNGYISGSVLAQTFLLAGVGTVLGLAGTYLSSLVLPSAVPFENNWTFYIAIGLALV
                     VFALLGASFSVRSIFKVDPLRNLS"
     gene            5907..6575
                     /locus_tag="ACHU66_001412"
     CDS             5907..6575
                     /locus_tag="ACHU66_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3389689.1"
                     /translation="MKTLIFENISKTFQDGSQTITALKPINFSIEEGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPTTGRVLINQSDYSDLPEKKRAKLRYKDIGFVLQASNLIPFLTV
                     EKQLTLVDKVNKKADPSKRNELLSELGVDHLKNKFPKDLSGGERQRVAIARALYNDPA
                     LILADEPTASLDSNRAFEVVELLAKEAKERNKSIIMVTHDQRMIEKCDKVYEMKDGVL
                     TQIR"
     gene            6944..8305
                     /gene="murF"
                     /locus_tag="ACHU66_001413"
     CDS             6944..8305
                     /gene="murF"
                     /locus_tag="ACHU66_001413"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR3389690.1"
                     /translation="MKLTLHEVAQVLGAKNDISLYPDRAFNKVEFDSRLITEGDLFVP
                     LKGARDGHDFIPVAFENGCVVTLSEVSLDVPHILVEDCLAALQQLAAYYLEKTGVEVI
                     AVTGSNGKTTTKDMIHDVLATTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKLVLEMGQ
                     DHLGDIHLLSEIAKPSLSVITLFGEAHLEFFGSREEIAKGKLQIADGMEPGSKLLIPA
                     YPIADKLLPSHVDLIRFGEQADLQVTSLVESKDSLTFTVNFMDGDIFLPVTGKYNATN
                     AMVASYVGKTLGVSNEAIKSALASLNLTRNRTEWKKAANGADILSDVYNANPTAMRLI
                     LETFSSIPANEGGKKIAVLADMKELGSQSVELHNQMILSLSPDVLDTVIFYGQDIEGL
                     AQLASQMFPIGKVYFFRKNAEQDQFGDLVKQVKEVLGGKDQILFKGSNSMNLGKVVEA
                     VEK"
     gene            8396..9094
                     /locus_tag="ACHU66_001414"
     CDS             8396..9094
                     /locus_tag="ACHU66_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HFR3389691.1"
                     /translation="MSDFFSNQISEAPNLNVLEHLFLMIIWFFMLHLCFRCYQKRWFH
                     IVFWWLQFIQIVSLYTWYIVADSPLSESLPFYHCRLAMLFILWAKPGPLKRYFAYLGL
                     FGSLAAFIHPVFDPFAFPHLTFFTFVIGHYALTVNCMLYLLSDLEGEMLKGKEVVKYT
                     LIMNMLILGVALLTGGNYGFLRQAPLVNTNNLPLNFFLVTCLLCFSILSIQAMLIAYL
                     KKESKQMNSHILKK"
     gene            complement(9125..11461)
                     /locus_tag="ACHU66_001415"
     CDS             complement(9125..11461)
                     /locus_tag="ACHU66_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFR3389692.1"
                     /translation="MNKQIKGLSTKEVQERIQNQQTNHFKTKTSASNWEIFRRNVFTS
                     FNALNFAIFLALLAVQAWSNLFFFGVIVLNAVSGMLTEWRARRMIDKLNLMNKDFIRV
                     VRDSKTLSLAPEDIVLDDVLLLSAGEQVPSDAIVLEGIAEANEAMLTGESDLIVKNTG
                     AELLSGSYLVSGQIYAKVIHVGAENYANKLMLEAKTHKPIVSRILYNMDKIAKFTGKI
                     IIPFGLALFLEAFFIKLLPLKDSVITSSTALLGMLPKGIALLTITSLLTAVIKLGMKN
                     VLVQEMYSVETLARVDVLCLDKTGTITQGKMTVENLLPLTNHYSLDTIQQILATYIQT
                     SEDTNSTAQAIRKEYGDLEHHYKASHIIPFSSDRKWGAMTIENIGRIFLGAPEMLLTQ
                     NPPSVSEAQARGSRVLILALSQQSLPSSQNQLPENIEPLALLEIADPIREDAAETLAY
                     LRSQEVTLKIISGDNPVTVSHIAKEAGFADYDSYIDCSKVDDEELIARAETTAIFGRV
                     SPHQKKLLIQTLKAQGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLD
                     SEFRDIPEILFEGRRVVNNISHIAPIFLIKTIYSFLLGLICIASIALGKAEYLLVFPF
                     IQVQMTLIGQFVEGFPPFILTFERNIRPVEKHFLRKSLLLALPNALMVVLSVLIFHLM
                     QVFGYLNLHDMQTLSYYVLGSTGLLAVIRACLPLTKARLALIIYSVFGFFISSHFLHG
                     LIEIHPLNSHTLPIYSGLMLIIIPVFFWISYKQGAFKN"
BASE COUNT         3360 a         2136 c         2587 g         3390 t
ORIGIN      
        1 gctcccaagc agttgagcct tcaagctggt caaaaggttg gtttgcacag gatttgataa
       61 accttgatga agaaaagact gcttttgaaa atgttagttg tacctctctt caagatagga
      121 tgacgattct caatttatta ggtatgttag gactttctta cgataaggct cagcagaagg
      181 tagcaaatct gtctggtggt gagcgtgttc gtctctcctt agctaaggta ttgttggcgg
      241 atgcgaatct tttgatatta gatgaaccga caaattttct agatatggca gctattgagg
      301 ctttggaaac attcttgaaa gaatatgaag gaagtgtcct cttggtttcc catgatcaac
      361 agtttgtgga aacatctgtt cacaggcaat gggagatagt gcaagcttgt ctcgtgaaga
      421 aatcataaga aaatattttc tgaaaaggag gctctgtcta aaaaatattt tttgcctgtg
      481 gtataataag aaggatattt ctagaatgag gtctttgaag tgaaaaaaag aaagaacaag
      541 cctttggaac acgatggaaa ttttatttcc cttcgtttaa atattttatt ttctatcgtg
      601 attttccttt tcctagtttt gatattgcgt ctggcagata tgcagatcat caatcatgat
      661 ttttatagta ataaactgtc tactgccagt cagaaaatca tcagcaatgg ctctattcgc
      721 gggcagattt acgatgcgca gggaaaacct ttggttgaaa acgagattca gcaggtcgtc
      781 tccttcacac gtcccaacaa gatgtctgct caggaaatga aagaagtagc taataaactc
      841 ttgcagtggg tcaatgtttc agacgtaaca attactcaac gtgataaggc tgattattat
      901 ttggcagatg aagaggttta tcgtcaggta gtcgaggcct tgccaaaaga taaaaaatac
      961 gattccgatg gtaactactt gagcgaatca acaatttatt ctaatgcagt tgatagtatc
     1021 cctgccgaag cattacaata ttctgagcaa gacaataaag cgattgaact cttcaagcag
     1081 atgaatggag ctacttactt ctctactgtt aatttggtaa cggatactct gacttctgaa
     1141 caggttgctc atatcgtagc caatgaaagt caacttccag gcatttcaac gaccaataac
     1201 tggcaacgga cgattttacc aacttccctc agctccatta tcggtacagt aacgagtgaa
     1261 caggctggtt tgcctgcaga agatgcagaa tactatctat ccaagggcta ctcttcaaat
     1321 gaccgtgtgg gaactgccta cctcgagaaa caatatgaag aagtcttgca aggtcaacgt
     1381 gagaaaaaac aaataaacct agatagaaat ggaaatatcg aaagtattga gacaatccaa
     1441 gaaggacagc agggaaataa tattaaactg actattgatt tagcctttca agatggggtc
     1501 aatgctattt tgagaaaata ctttgaaagt gagctagcta ctggtagtgc gctttactcg
     1561 gaaggtgttt atgcagttgc attaaatcct tctacaggtg cagtacttgc tatgtcagga
     1621 tatagtcacg aaaaaggttc aagtactatt acggaggatg ctcttggtac cattacaagt
     1681 gtctttacac caggttccat cgtcaaagga gcgacaatca gctctggttg ggaaaatgga
     1741 gttatcagtg gtaatcaggt tcaattagat gaaccaatct attttgcagg ttcagcacca
     1801 attacatctt ggtatggggc ctatggtagt tttaatattg atgcgacaga ggctctggaa
     1861 tattcttcta acatctatat ggttaagatt gctttgggac tactcggtca gacctactca
     1921 gctaacatgt atttgaatga tggtgataca ttgaaaaacg ccatgactaa gcttcgttcg
     1981 acttttgcag aatatggtct tggtgcttct acaggtattg accttccact cgaatctaca
     2041 ggatttttac cagataagta tacgacagcc aactttatta ccaacgcttt tggacagttt
     2101 gataactata caccgatgca gatggcacag tatgcagcga ccattgcgaa taatgggaca
     2161 cggatttctc ctcacctcgt tgagggaatt tatgggaata atgcccaagg tggtttggga
     2221 gaattaattg agaccgtatc tggtaaggag atgaatcagg ttaatatctc cgctgaggat
     2281 atgtcacttc ttcgtcaagg tttctaccaa gttgtcaatg gtagtgggcg ctttaataca
     2341 ggtagtgcta tcggtcgtgg tgcggctgtg accatcagtg ccaagactgg tacagccgaa
     2401 acctatacaa caacagcttc aggagatgtg gttactgcga tcaacaccaa cgttgttgct
     2461 tatgcgccta gtgataatcc tcaaattgcg gtagcagttg tcctaccaaa tttgaccaat
     2521 caaagttcaa caactactaa aacaattact caagaaatta tcaatttata tcaatcactt
     2581 cacccaatga attaaggagg cttatgcttt accctacacc tattgccaag ttaattgata
     2641 gctattctaa attgccaggt atcggtataa aaacggctac ccgtttagct ttttatacta
     2701 ttggcatgga ggatgatgtc gttaatgagt ttgcaaaaaa tttattggca gccaagcgag
     2761 acctatctta ttgctctatt tgtggtaatt tgacagatca ggacccctgt gctatttgcc
     2821 aggactcaac gcgagaccaa tctactattt tgattgtaga ggatagtcga gatgttacag
     2881 ccttggaaaa tattcaagaa taccacggtc tttatcatgt cttgcatggc ttgatttctc
     2941 ccatgaatgg tattggacca gatgatatta atttgaaaac cctcctcact cgcctgatgg
     3001 acaatgaggt tacagaagtt attgtggcga ccaatgcaac agcagatgga gaagctacat
     3061 ctatgtatat ctcacgtgtc ctcaaacctg cgggaatcaa agtaacccga ctagctcgtg
     3121 gcttagcggt aggcagtgat attgaatatg cagatgaagt gaccctgttg agggctattg
     3181 aaaaccgcac agagctatag ctgttctgta gttagatacg ttcctaattt taagatagga
     3241 tagatatgat ttgttaaatt atgctatact aagagtaata gcgttgatac aggaaggaaa
     3301 atatatgtca aaagaaacct tgattttact atatggtggg cgttctgcag agcgtgaggt
     3361 atctgttctg tctgccgaaa gcgtcatgcg tgctatcaat tatgataaat tttttgtcaa
     3421 aacctatttc attagccaga caggggactt tattaagacg caagagtttt ctcaaacacc
     3481 gtctgccgat gaaaaactca tgacaaacgc gacgattgtt gaagagcaaa aaatccgtcc
     3541 gagcgatatt tatgaagaaa aggcggttgt tttccctgtt cttcatggcc caatgggaga
     3601 agacggatct attcaaggct tcttggaagt gcttcgtatg ccttatgttg gcactaatat
     3661 cttgtcttca agcgttgcta tggacaaaat cactaccaag cgcgtacttg aatcagcagg
     3721 aatctcccaa gtaccttatg tggcagttat cgagggtgaa aatattgacg ataagatcgc
     3781 agagattgaa ggaaaattga cttatccggt ctttgtaaaa ccagctaata tggggtcaag
     3841 tgtcggtatc tcaaaagctg aggatttagc tggcttgcat aaggcacttg atctggcctt
     3901 caagtatgac agccgtgtcc tcgttgagca gggtgttaat gcgcgtgaga ttgaggtcgg
     3961 tcttttggga aatgctgacg tgaaaaccac ccttcctggc gaagtagtca aagacgttgc
     4021 tttttatgac tacgatgcca aatacatcga caacaaaatc accatggaaa tcccagctaa
     4081 gattgacgag gctatcatgg acaccatgcg tgataatgct gccaaggcct tccgtgccat
     4141 cggtggttgt ggactttctc gctgtgattt cttcctgaca gaagacggcg acatcttcct
     4201 gaatgagctc aataccatgc caggctttac ccagtggtct atgtatccac tcctttggga
     4261 taatatgggc ctagcttatc cagacttgat tgaagagctt gttcgtttgg ccaaagaaat
     4321 gtttgaaaaa cgcgaaagtc acctcattta attattatca agattggaat cattccaatc
     4381 ttttttcttg caaaggtttg caaaaaggaa taaaatggaa caagttctgg aggtgtttat
     4441 gtatcaaacg atagtaaaag gggaaggtct gtttcaggct ctttctcaag gttatggcga
     4501 atcaattcgt acctttgggg taacggagaa gggtgagact ccggttagtc tagttaatat
     4561 tggcttagct gcctgtatca ccatgtgtgt gcagggctac tatgccagcc aagaaggcaa
     4621 taaaaccatg cctgttcagg tggagagtcg cttggacaat cagcgttttg aggtgttgat
     4681 tggaattggt gagtcggttt cagcgcagaa gcagaaagag attcttgcct atgtggaaaa
     4741 gaaatgtaaa gtcaaagctc ttttgaggga agacttgcag tttgagacaa gtttttatgt
     4801 cttagaaagt gtggagtaag atgtttttag caattgaaga aatgcgacaa aataagctgc
     4861 gttatggctt gattttagga ttattaatcc tgatttccta cctggtcttt ttcctgacgg
     4921 gtttggctta tggtctcatg caggaaaata gaacagcagt tgataaatgg caggcagatt
     4981 atgtcttgct taattcagaa agcaatcgct tgataacagc atccaaaatt gatacgagtc
     5041 tgcttgatca ggtggatgct agtgacaagg ccttgattcg ccaacaagct ggagttgcct
     5101 atgtggatga aaatgccacc tcagacgaaa aagaaaaggt aaacatcttt gctgttgaat
     5161 cagatagttt tatcgttcca aatattgtag aaggtcgttt gtatgaaaaa acgggagaag
     5221 tccttgtaga caaaaccttg tcagaagtag aaggttttgg gcttggagat caaatctatc
     5281 tgtcaggttc tgatgaaaaa gtgactattg taggttatac agacaatgct tattttggag
     5341 tggcgcctgt tgtatatatg gactttacgg catttgcaga attgatgcag gctgataaac
     5401 aacaagctgc aacgagtaat cttgctagtg ccattgtcgt tcgtggcgat gccgcttctc
     5461 taccaaatga gctggaaaaa gttgccattg cagactttat tgaaaatcta ccaggatata
     5521 aggcacaaaa tatgaccttt ggctttatga taggattctt gattgtcatt tctgccattg
     5581 ttattggtat ttttatcttt gttttgacaa cacagaaatc tccgattttt ggcttgatga
     5641 aaattcaagg tctttcaaat ggctatattt caggttctgt attggctcaa accttcctac
     5701 ttgctggtgt gggaacagtc cttggattgg ctgggactta tttgtcatcc cttgtcttgc
     5761 caagcgctgt tccatttgag aataactgga ccttctatat tgcaatcggc ctagcactag
     5821 ttgtatttgc cctattgggt gctagtttct ctgtacgttc tatctttaag gtagatccat
     5881 tacgaaatct atcttaggag gttgttatga aaacacttat ttttgaaaat attagtaaga
     5941 ccttccaaga tggcagccaa accattacgg ctcttaaacc aataaatttt agtattgaag
     6001 aaggcgaatt tgttgccatc attggaccat ctggttctgg gaagtcaaca tttttgacct
     6061 tggcaggtgg tttacaaaca cctacaacgg gtcgtgtttt aatcaatcaa tcggactatt
     6121 ctgatttacc tgagaaaaaa cgtgccaaat tgcgctataa ggacattggt tttgtcttgc
     6181 aggcttccaa tttgattcca tttctaacag tcgaaaaaca attaaccttg gttgataagg
     6241 tcaataagaa agctgatccg agcaagcgaa acgaactcct atctgaattg ggtgtggacc
     6301 atctcaaaaa caagttccct aaggatttgt caggcggaga acggcagcgt gtagctattg
     6361 ctcgtgcact ttataacgat ccagctttga ttctagctga tgaaccaaca gccagtctag
     6421 acagtaatcg cgcttttgaa gtggttgagt tattagctaa ggaagccaaa gaacggaaca
     6481 aatccatcat catggtaaca catgaccagc gcatgattga aaaatgcgac aaggtttacg
     6541 agatgaaaga tggtgtcctc actcaaattc gataaaaaat ggtataatgg gagcgggaaa
     6601 gaactcgact tgtcaaaaaa gagttcgtca acaagttttt atttctagtt gttgagctga
     6661 aacagtctat tcccagactg tttcactccc acccccgcac agctcaaact gtctgggaga
     6721 cagtttgagg ttggaaataa agcgaacact gatcgcatca gtaggtagga cagatttgga
     6781 gtgtaattac gaacagttga aatttgcttc gcaaatccca tctccaacct ttaatagtcc
     6841 actggactat taaagccaat caaccactgc gctgggatgt tgacgcaaat tttgagacgc
     6901 ggtgtggggg tggaaactag cctttagttt attggagaaa acaatgaaat taaccctaca
     6961 cgaagtcgct caggtcttgg gcgcaaaaaa tgatattagt ctttatcctg atagagcatt
     7021 caacaaggtc gagttcgaca gtcgcctgat tacagaaggg gatctttttg ttcccctcaa
     7081 gggagcgcgt gacggtcatg actttattcc tgtggctttt gaaaatggct gcgtagtaac
     7141 cctgtctgaa gttagccttg atgttcctca cattctagta gaggactgct tggctgcgct
     7201 tcaacaatta gcggcttact acctagagaa aacaggggta gaagtcattg ctgtgacagg
     7261 atcaaacggt aaaacgacca ccaaggacat gattcacgat gttctggcga cgacttacaa
     7321 gacttataag acacaaggca actacaacaa cgaaatcggt ctgccataca ccgtcctcca
     7381 catgcctgat gatacagaaa aactggtctt ggaaatgggc caagaccact tgggagatat
     7441 tcatcttcta tccgaaattg ccaaaccaag tctatcagtt attacccttt ttggtgaagc
     7501 gcatttagaa tttttcggct cacgagaaga gattgccaaa ggaaaattac agattgcaga
     7561 tggaatggag ccaggtagta aactcttgat cccagcatat ccgattgcgg ataagctctt
     7621 gccgagccat gtagacctca ttcgcttcgg tgagcaggct gatttgcagg tgactagtct
     7681 ggtcgagtca aaagatagcc tgaccttcac cgtcaatttt atggacggcg acattttcct
     7741 cccggtaacg ggcaagtaca atgccaccaa tgctatggta gcaagctatg tgggcaagac
     7801 cttgggtgtg tctaatgagg ccatcaagtc agccctagcc agtctaaact tgacccgcaa
     7861 ccgtaccgag tggaagaagg cggcaaatgg agcagatatt ctcagcgatg tctacaatgc
     7921 caatccaacg gctatgcgct tgattttaga aaccttctct agtattccag ccaatgaagg
     7981 cggtaagaaa attgctgtgt tagcagacat gaaagaactt ggcagtcagt cggttgagct
     8041 tcataatcag atgattttga gcttgtcgcc tgatgtgcta gacaccgtca ttttctacgg
     8101 tcaggatatt gaaggcctgg cccagttggc tagtcagatg ttcccaatag gaaaagtcta
     8161 tttctttagg aaaaatgcag agcaggacca atttggagac ctggtgaaac aagtcaagga
     8221 agtcctcggt gggaaagacc aaatcctctt taaaggctct aattccatga atcttgggaa
     8281 agtagtggaa gcagtagaaa agtagagagg cgagacctct cttttttggc aagtcttgtc
     8341 aaaacatcga aaagttgata tactgacttt tggggaaaga gaggaattta ataagatgag
     8401 tgattttttc agcaatcaga tttctgaagc accgaacctg aacgtattgg aacatctgtt
     8461 tctcatgatt atttggtttt tcatgctcca tctttgtttt cgttgttatc aaaaaagatg
     8521 gtttcatatt gttttttggt ggcttcaatt tatacagatt gtctcccttt atacttggta
     8581 tatcgtagct gattctcctc tttcagagag tcttcctttt tatcattgtc gcttggctat
     8641 gctttttatt ctttgggcga agcccggacc tttgaaacgt tattttgcct atctcggtct
     8701 ttttggatcg cttgcggctt ttattcatcc agtatttgac ccgtttgctt ttccacacct
     8761 taccttcttt acttttgtta ttggtcacta cgccttgacg gttaattgta tgttgtacct
     8821 gttatctgat ttagaagggg agatgttgaa gggaaaagag gtagtgaaat atacgcttat
     8881 tatgaatatg ctgattctag gagtagctct tttaactggc ggaaattatg gatttttgag
     8941 acaggcacct ttagtgaata ctaataatct tcctttgaat ttcttcttgg taacctgcct
     9001 actctgtttc tctattttga gtatacaagc tatgttaatt gcctatttga aaaaagaaag
     9061 caaacaaatg aattcgcata tattgaaaaa ataagacttg aaattagttt ttcaagtctt
     9121 atttttagtt tttaaatgcg ccttgtttgt agctaatcca gaaaaagaca gggataataa
     9181 tgagcattaa tccagaatag attggcaatg tgtgactgtt tagggggtgg atttcaatca
     9241 gaccatgtag gaaatgggag ctgatgaaga aaccgaaaac ggaataaata ataagagcca
     9301 gtcgagcttt tgtcagtggc aaacaggcac gaatgacagc cagtagacct gttgaaccaa
     9361 gaacataata agagagggtt tgcatgtcat gcaagtttaa gtatccaaat acttgcatta
     9421 aatggaaaat aaggacactg agaacaacca tgagagcatt tggtagggct aagagaagag
     9481 attttctgag aaaatgtttt tctacggggc ggatatttcg ttcaaaagtt aggataaaag
     9541 gaggaaaacc ttcgacaaat tgaccaatga gggtcatttg tacttgaata aatgggaaaa
     9601 ctaacaaata ttctgcttta cctagggcaa tactagcgat acagataagg ccaagtaaga
     9661 aagagtagat tgttttgata agaaagatgg gagcaatgtg ggagatgtta ttaacaacgc
     9721 gtcgtccctc aaagagaatc tcaggaatat cgcgaaattc agaatctagg aggactagat
     9781 tagcgatttg tctagtagcc ggatcgcctt ctgccataac aatggaacaa tcagcttcgc
     9841 gtagtgcaag aatatcattg accccatcac cggtcattgc agtagtatgt ccctgtgctt
     9901 tgagggtttg gatgagcaat tttttctgat gtggggatac gcgaccgaaa atggcggttg
     9961 tttcagctct ggcaattagt tcttcgtcgt caactttgga acaatcgata tagctatcgt
    10021 agtcagcaaa tccagcttcc ttagcaatgt gtgagaccgt cacaggattg tcaccagaga
    10081 ttatttttag tgtcacttct tgcgagcgaa gataggccag tgtttctgcg gcgtcttctc
    10141 ggatagggtc agcaatttct aatagagcta gaggttcaat attctcaggt agctgatttt
    10201 gagaagaagg aagggactgt tggctaagcg caagaatcag gacacgggaa ccacgagcct
    10261 gtgcctcaga aacagaagga ggattttgtg ttagtaacat ttctggtgcc cctagaaaaa
    10321 tgcgaccaat gttttcgatt gtcattgctc cccattttct atcgctagaa aatggaatga
    10381 tgtgactagc cttatagtgg tgctctaaat caccatattc ttttcgaatg gcctgagctg
    10441 ttgaattagt atcctcactt gtctggatat aggtagcgag tatctgttgg atagtgtcaa
    10501 gtgaataatg gttggttaag gggagcaggt tttcgacggt cattttcccc tgagtaatcg
    10561 ttccggtctt atctaaacaa agaacatcaa ctcgggcaag tgtttcgacg gaatacattt
    10621 cttgtacgag gacatttttc attcccagtt tgataacagc agttaagaga gaagtgatgg
    10681 tcagcagagc aattcccttt ggtaacatac ctagtagggc agtagagcta gtaataactg
    10741 agtcttttaa gggaagaagt ttgatgaaaa atgcttctag aaaaagagca agtccgaatg
    10801 gaatgataat ttttcctgta aatttagcaa ttttatccat attgtaaaga atacgagaga
    10861 cgataggttt gtgagttttc gcctcaagca tgagcttatt ggcatagttt tccgcgccaa
    10921 catggatgac ttttgcataa atctgtccgc taactagata gctaccagac aataattcag
    10981 ccccagtatt tttcacaatc aagtcgcttt caccggttaa catggcctcg tttgcctcgg
    11041 ctataccttc taaaacaata gcatcactag gaacttgttc gccagcagac agcaagagaa
    11101 catcatctaa aacaatatct tctggtgcaa gagagagtgt cttactgtca cgaacaactc
    11161 ggataaaatc cttgttcatc aggttgagtt tgtcaatcat tcgtcgtgct cgccattctg
    11221 tcagcattcc tgaaacagcg ttgaggacaa taactccgaa gaagaaaagg ttggaccatg
    11281 cctgaacagc tagtaaggct aagaaaatgg caaaattgag tgcgttaaaa gatgtaaaca
    11341 catttcgtct gaagatttcc caattactag cactggtctt tgtcttaaaa tgattggttt
    11401 gctggttttg aatacgttct tgtacttcct tggttgataa accttttatt tgtttgttca
    11461 ttattttatc ttt
//
LOCUS       DAZQCJ010000060        11188 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000060 DAZQCJ010000000
VERSION     DAZQCJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11188
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.061"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            39..854
                     /locus_tag="ACHU66_001416"
     CDS             39..854
                     /locus_tag="ACHU66_001416"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFR3389693.1"
                     /translation="MNQQFSLDQFSLKGKIALVTGASYGIGFAIASAYAKAGATIVFN
                     DINQELVDRGMAAYRAAGINAHGYVCDVTDEAGIQAMVAQIESEVGIIDILVNNAGII
                     RRVPMIEMTAAQFRQVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRETV
                     SAYAAAKGGLKMLTKNIASEYGEANIQCNGIGPGYIATPQTAPLRELQKDGSRHPFDQ
                     FIIAKTPAARWGEAEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPEA"
     gene            906..1547
                     /locus_tag="ACHU66_001417"
     CDS             906..1547
                     /locus_tag="ACHU66_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFR3389694.1"
                     /translation="MKIALINENSQAAKNSLIFETLKSVTDAKGYHVFNYGMYGKEGE
                     SQLTYVQNGLLASILLVSKAADFVITGCGTGVGAMLAANSFPGVVCGFAVDPTDAYLF
                     SQINGGNALSLPFAKGFGWGAELNLKLLFERLFAEEMGGGYPRERVIPEQRNARILNE
                     MKAVTYKDLLTVLQEIDQDFLKETISGEHFQEYFFANCQDESIAAYLKTVLES"
     gene            1564..2565
                     /locus_tag="ACHU66_001418"
     CDS             1564..2565
                     /locus_tag="ACHU66_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR3389695.1"
                     /translation="MKKVLLIGEPLIRITPTNYQELTDGVESRVFFGGSEINVACNLQ
                     GFGCETKLLTALPQGSLGDRFLDFLDGKGIDTSSVQRKGERVGVYYMEAGFGCRSSQV
                     YYDRGQSSLEAIQVEHFDMEQLFLEVGLVHFSGITVAIGSAVRAWLVQVLKEAKRRKI
                     PISMDLNWRSKMIGLRDAKKVFSQFATYADYCFGLEPIMANEKDWELFERDTATVHTI
                     RERMQTLKEAYKFQAIFHTIRQTDELGRNLYKAYGLADEFCCSQELKTQVLQRVGSGD
                     AFVAGALYKLLEGTSLKAVLDFAVASGTYKCSIEGDHMRQSLSKVESLLEGKNDIIR"
     gene            2576..3217
                     /locus_tag="ACHU66_001419"
     CDS             2576..3217
                     /locus_tag="ACHU66_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR3389696.1"
                     /translation="MSPSQIISKLKEQGLVVVVRGDDKEVGFKTAQACIAGGITAIEV
                     AYTNPDANMIITELSRCHKDDVSVLIGAGTVLDSVTARQAIMAGASYIVSPSFNIEVA
                     KICHLYAVPYLPGCMTLTEITNALEAGCEMVKLFPGSVLGTNYITAIKGPLPHVSIMV
                     TGGVKLDNVSEWFNAGATVLGIGGEFNQLATKGEFGTIQELASSYASVRDKMK"
     gene            3503..3937
                     /locus_tag="ACHU66_001420"
     CDS             3503..3937
                     /locus_tag="ACHU66_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIA"
                     /protein_id="HFR3389697.1"
                     /translation="MIKVIVVAHGQFASGILTSLKLIAGEVENIEAIDFSEGMSAQEL
                     KARIKSAILGEREVLILTDLLGGTPFKVSVELAAEQKEQNVVVLSGLNLAMILEANFS
                     RLTDDLEQLVGKLITTSKDGIVDSVSLLAEDNHDEELFEDGI"
     gene            3949..5139
                     /locus_tag="ACHU66_001421"
     CDS             3949..5139
                     /locus_tag="ACHU66_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFR3389698.1"
                     /translation="MIKDVLIESIAKKREFRNSTLLSKKEVEAAIELAIQQLVINCEY
                     FGESFPTPATFDNHYPKMDNTEWTNGFWTGELWLGHEYSLNNEMKALAKKNTTSFLER
                     VKQRIELDHHDLGFLYSPSTVADYKLTGSKEALEASLLAADKLMERYQEKGGFIQAWG
                     QLGKAEDYRLIIDCLLNLQLLFFAYEETGEQKYYDVALSHFYASVNTVIRDDASTFHT
                     YYFDPETGKPLKGVTRQGFSDDSSWARGQSWGIYGIPLAYRKIKDPACLNLFKGVTNY
                     FLNRLPEDNVSYWDLIFNDGSGHARDSSATAIAVCGIHEMLKYLPEVDEDKETYKYAM
                     HTMLRSLIDNYANRELAPGRPLLLHGVYSWHSGKGVNEGNTWGDYYYLEALLRFYKDW
                     ELYW"
     gene            5149..5640
                     /locus_tag="ACHU66_001422"
     CDS             5149..5640
                     /locus_tag="ACHU66_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR3389699.1"
                     /translation="MATPNIVMARIDERLIHGQGQLWVKSLGCNTVIVANDKVSEDKI
                     QQTLMKTVIPDSVAMRFFSLQKVIDVIHKANPAQTIFLVIKDLQDALTLVKGGVPITE
                     LNIGNIHNAPGKTQVTRSIFFGSEDKAIVRELNDVYKIRFNTKTTPSGNDGAVEVNIL
                     DYI"
     gene            5660..6436
                     /locus_tag="ACHU66_001423"
     CDS             5660..6436
                     /locus_tag="ACHU66_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFR3389700.1"
                     /translation="MTISLIQAALIGLWTAFCFSGLLLGLYTNRCIVLSFGVGIILGD
                     LPTALAMGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVTPEAALALST
                     PIAVGIQFLQTSAYTLHAGAPESAIKHLRAGNLSKFKRVANGTVITFAFLGFALGFLG
                     AYSMDTLLKLVEMIPPVLLTGLSVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLQLPVIGIAIVGSIFALKEFFNKPEQVAVKQEEAHDDWI"
     gene            6423..7238
                     /locus_tag="ACHU66_001424"
     CDS             6423..7238
                     /locus_tag="ACHU66_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR3389701.1"
                     /translation="MTGSKLTKKDYLNTSLRAFFLQNGFNYSNYQGIGYANVMYPAFK
                     KHFGDDKEGLTKALEENCEFYNTNPHFLPFITSLHLVMLENDLPEEETRGIKMALMGP
                     LAGIGDSLSQFVLAPLLSTIAASLAGQGLVMGPILFFLGMNIILTSIKLLTGLYGYKF
                     GTSVIDKLSDQMATISKVANIIGVTVISGLAVTFVKISVPITFAAGELTEGADQKIVT
                     IQGMLDKIAPALLPALFTILVYYLIKNKKWTTYKLVILTVIIGILGSWLGILA"
     gene            7245..7553
                     /gene="yajC"
                     /locus_tag="ACHU66_001425"
     CDS             7245..7553
                     /gene="yajC"
                     /locus_tag="ACHU66_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR3389702.1"
                     /translation="MEQQTLYYVLIILVALSTILPVLTSYINRKRQQKALEEQLEKRQ
                     RYLSTLQAGDEVILFSGLHGKIIKLTDSLVELQIAEGVLVYVEKESIMGKAKELLFTK
                     "
     gene            7650..11144
                     /gene="hylA"
                     /locus_tag="ACHU66_001426"
     CDS             7650..11144
                     /gene="hylA"
                     /locus_tag="ACHU66_001426"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HFR3389703.1"
                     /translation="MGFFISQSKQHYGIRKYKVGVCSALIALSILGTRVAANQLPSAE
                     TASPQSSQLVETTPETTEAVMTSEVSSEVSPVTSTETQPSSTTAETLASPQAVQATKE
                     EKNLVANGEFISTTAPSGNWKELAATNWETWIPANVKKENGQVRIDEGRLHISSTASY
                     RVAVHQTVDVDPNKRYLFSYDVETKDLKGSGVRVRLRSLTAEGKDLSPQEFAYTPYKN
                     GSQAEHIEQILTVSPETRKLKVELFFENSVGQAWLDNISLVEYVEKTPETPEQSPELV
                     QPETGQISLASNKVYLPVRPDLTYRIADAAVATVEKNMIRPLAAGKTQVDVYDKDTKL
                     SSFELIVTEHQATVFDTLRNNWEDISLANKRYQSNDAQMKAFLGRLDAGVASSLEKWV
                     EPTEQSKTIFNDIDFSKSSHLTTVYRRLEQMAQVVENPDSAYYHDRSLIDLVRKGMNW
                     LYTNVYNENKSIDGNWWDYEIGTPRAVVNTLIYMHPYFSQEEILTYTKPISKFVPDPT
                     TIRKTLTNPVPAVGGNQTDLSKVAILEGALREDADRVRAGAQGLTTIMKFVDKGEGFY
                     RDGSFIDHTNVAYTGAYGNVLIEGFSQLLPVIQPTEFALKEEQTNILYEWIEKAFMPI
                     LVRGELMDMTRGRSISRATGESHVQAMEILRSLVRIAESAQPEQKTKLLSFVKAQLTS
                     DTFYDSYRSLKSYKDIDLVNKLLADNQIPAEVDKDYIAAFNNMDKFVYRSAQEGFTFA
                     LSMYSSRTQNYEDMNNENRKGWYTADGMVYLYNDDLSHYSNHYWATVDPYRLPGTTTT
                     KDKREDGSGEVTLASDFVGASQLGNRLATIAMDFNNWNNSLTARKAWIVLGNKIVFLG
                     TDIQHQSAQGAETTIENRKLLTGEKYSYYINGQPVDLSKEVVTDKTQSFYMTNGKDNQ
                     SIGYVFLNQLPTHAKLDQRTGKWSDINYNQSKEEVSNSFVSLWHEHAQTSSNYAYVLV
                     PNQSMEKVNQAAASVKLLHQDRDLQVVYDQEQNVWGVVKYTDTAYKLTDDITLTDAGL
                     YTIQKVEGGYRIAFYNPSTRTVKNGIELTKAGSSLTVEMEPTAAYPSTVWKVTMPEGS
                     DKQTGSVEKTEKEEKQLKENQPSSEVKQVVHHAAEKTKPSKPRLPQTGEEASLGLGFL
                     GLLTLGAVVDFKCRRSHS"
BASE COUNT         3392 a         1967 c         2623 g         3206 t
ORIGIN      
        1 aaacgcttta caaatcaaaa tacaaaggag ttacgaatat gaatcagcaa ttttcattgg
       61 atcaattttc tctaaaggga aaaattgctt tggtaacagg ggcgtcgtat gggattggct
      121 ttgcgattgc atcggcctat gcaaaagcag gggcgacaat tgtttttaat gatattaatc
      181 aagagttggt tgatagggga atggcagcct atagggcagc tggtattaat gcgcatggct
      241 atgtttgcga tgtaactgat gaagctggaa tacaggctat ggttgctcaa attgaatcag
      301 aggttggaat tattgatatt ctagtgaata atgctggtat tattcgtaga gttccaatga
      361 ttgaaatgac agcggctcaa tttcgccaag ttattgatat tgacctaaat gctccgttta
      421 ttgtttctaa agcggttatt ccatctatga ttaaaaaggg gcatgggaag attatcaata
      481 tttgttccat gatgagtgag ttaggacgag aaacagtgtc tgcttatgca gcggctaagg
      541 gtgggcttaa gatgttgacg aagaatattg cgtcggaata cggtgaagcc aatatacaat
      601 gtaacggcat tggtcctggg tatattgcaa ctccacaaac agctccgtta cgtgaactcc
      661 agaaggatgg ttctcgtcat ccatttgatc aatttattat cgcgaagacc cctgcagctc
      721 gttggggtga agctgaggat ttgatgggtc cagcagtctt tttagcaagt gatgctagta
      781 attttgtgaa tgggcacatt ctttatgttg atgggggaat tctagcttac attggtaaac
      841 agccggaagc gtaataaacg aattaacaaa tttaacaaat tttgatataa gaagggaaaa
      901 aaacgatgaa aattgcactt attaatgaaa atagtcaggc tgctaaaaat agtcttattt
      961 ttgaaacatt gaagtcagta acagatgcta aagggtatca tgtttttaac tatggaatgt
     1021 atggtaaaga gggagaaagt cagttgactt atgtacaaaa tggtctgttg gcttcaatcc
     1081 ttctagttag taaggcagcg gattttgtta ttacaggttg tggcacgggg gtaggtgcca
     1141 tgttggcggc taatagtttt ccaggggtag tttgtggctt cgcggtagat ccgacggatg
     1201 catacctctt ttcacagata aatggaggaa atgctttgtc tctgccattt gcaaagggat
     1261 tcggttgggg agctgaatta aatctcaaat tactttttga gcgactgttt gcagaggaaa
     1321 tggggggcgg atatccccgt gaacgcgtta ttccggaaca acgaaatgct cgtattttga
     1381 atgaaatgaa agcagtcacc tataaagact tactgacagt acttcaggaa attgatcaag
     1441 attttttgaa agaaactatt tcaggtgaac atttccaaga atatttcttt gccaactgtc
     1501 aggacgagtc aattgcagca tatttaaaaa cagtattaga aagttaaaga gtggggggcg
     1561 gttatgaaga aggtattact gattggtgaa cctttaattc gcattacacc aacaaattat
     1621 caagaattga cagatggtgt agaaagtcgt gtgttttttg gaggttcaga aatcaatgtc
     1681 gcttgtaatc ttcaaggatt tggatgtgaa accaagcttt tgaccgcact accacaaggc
     1741 tctctaggtg atagattttt agatttctta gatgggaaag ggattgatac ttccagtgtt
     1801 caacgtaagg gggaacgtgt gggggtatac tacatggaag caggatttgg ttgccgttct
     1861 agtcaagtct actatgatcg tggccagtct agtttggaag ctattcaagt cgaacatttt
     1921 gatatggaac aattattttt agaggttggg ttggttcact ttagtggtat caccgtggct
     1981 attggttcag ctgttagggc atggcttgtt caagtattaa aagaagcaaa gcgtcgtaaa
     2041 attccgattt cgatggatct taattggcgt tctaagatga ttggattaag agatgcaaaa
     2101 aaagtgttct cgcaatttgc gacctatgca gactattgtt ttggtctgga acctattatg
     2161 gctaatgaaa aagattggga attatttgaa agagatacgg ctactgtcca tacaataaga
     2221 gaacggatgc aaactttaaa ggaagcctat aagttccaag caattttcca tacgattagg
     2281 cagacagatg aactaggcag aaatttgtat aaggcttacg gattggctga tgaattttgt
     2341 tgctctcaag aattgaaaac tcaggtgtta caacgtgttg gcagtggtga tgcgtttgtt
     2401 gctggggcat tatataaatt gttagagggg acatcgttaa aggctgtttt agattttgca
     2461 gttgctagcg ggacttataa atgcagcata gaaggggatc acatgcggca atctcttagc
     2521 aaagttgagt ctttattaga ggggaaaaat gacattatac gataaaggag aaaaaatgag
     2581 tccatcacaa attatttcaa aattgaagga gcaagggctt gttgtagtcg ttagaggaga
     2641 tgacaaagaa gtcggattta agacggcaca ggcctgtatt gcaggaggaa ttacagctat
     2701 agaggttgct tatacaaatc ctgacgcgaa tatgattatt acagaactta gtcgatgcca
     2761 taaagatgat gtttctgttt taattggggc tggaacggtt ctagattctg taacggcacg
     2821 gcaagccatt atggcaggag caagttatat tgtttcacca tcttttaata tagaagttgc
     2881 aaaaatctgt catctttatg cagtgccata tcttccgggc tgtatgactt taacggagat
     2941 tacaaatgct ttggaagcag gctgtgaaat ggttaaactg tttcctggta gtgttttggg
     3001 gacaaactat attactgcaa tcaaaggacc attaccgcat gtttcaatca tggtaaccgg
     3061 tggagtaaag cttgataatg tttcagaatg gtttaacgct ggtgcaactg ttcttggtat
     3121 tggtggggaa tttaatcaat tagctactaa aggagagttc ggtacaattc aagaattagc
     3181 gtcctcttat gcaagtgtta gagataagat gaagtagttt atttcaaaat aaggactgta
     3241 tttttaacgt tttcactttc tcctgcctcc aacggggttg aatgtgggag aaagtgagat
     3301 gttacagggg aaattttgaa cggaaacatg gagatatttg gtactcctgt ttatgaaaag
     3361 agggagctgg agaatatttt ttaaataaaa gtccaacaaa atataaaacg cttacatttt
     3421 ttataaaatc tattgacaag tggttgtcaa tagtctacaa tacaaagtgt aggtaaaccg
     3481 gtaaagttaa aaggagagaa ttatgattaa agtgattgtc gttgctcatg gacagtttgc
     3541 tagtgggatt cttacttcac taaagttaat tgcaggagag gtggagaata ttgaggcaat
     3601 tgattttagt gaaggaatgt cggcgcaaga actaaaagca cgcatcaaat cagctatatt
     3661 aggcgaaaga gaagttttga ttttaactga tttactagga ggtacgcctt ttaaagttag
     3721 tgtagaatta gcagcagaac agaaagagca gaatgttgtc gttctttcag gtttaaatct
     3781 tgcgatgatt ttggaagcga atttttcacg gttaacagat gatttagaac aattggttgg
     3841 taaattgatt accacctcta aagatggaat cgttgattcg gtcagtctgt tagcagagga
     3901 taaccatgat gaagagttat ttgaagatgg tatatgaggg agaaatgcat gattaaggat
     3961 gttttgattg agtcaattgc taaaaaaaga gagtttagaa attccactct attgagtaag
     4021 aaagaagttg aggctgcaat agaattggcg attcaacaac ttgttatcaa ttgtgaatat
     4081 tttggtgagt cgttcccgac tccggccaca tttgataacc attatccaaa gatggataat
     4141 acagagtgga caaatggttt ttggacgggc gagctttggt taggacatga gtattctttg
     4201 aacaatgaaa tgaaagcgtt agcaaaaaag aatacgacat ctttcttgga acgtgtcaaa
     4261 caaagaattg aattagatca tcatgatttg ggatttctgt attcaccatc aacggtagct
     4321 gattataaat tgacaggtag taaagaggct ttagaggctt cgctcttggc tgctgataaa
     4381 ttaatggagc gctatcaaga aaaaggaggt tttattcagg cctgggggca acttggtaaa
     4441 gcagaagatt atcggttgat tattgattgt ttgctgaatt tacaattact tttctttgct
     4501 tatgaggaga caggtgagca gaaatattat gatgtagctc ttagtcattt ctacgcttct
     4561 gttaatacag ttattcgaga tgatgcttca acatttcata cctattactt tgaccctgaa
     4621 acaggaaaac cgctcaaagg ggttacccga caaggattta gtgatgactc ttcctgggcg
     4681 cgtggtcaat cttggggtat ttacggtatt ccgttagctt accgtaaaat taaagatccc
     4741 gcatgtttaa atttgtttaa aggagtaacc aactactttc ttaatcgttt gccagaggat
     4801 aacgtttcct attgggattt aatttttaat gatggtagtg ggcacgctcg tgattcttct
     4861 gcgacagcaa tagcggtttg tggtattcac gagatgctta aatatttgcc agaagttgat
     4921 gaggacaagg aaacttataa atatgctatg cacacgatgc tacggtcctt gattgataac
     4981 tatgctaatc gtgagttagc tccaggtcgt ccattgcttt tacatggggt ctactcttgg
     5041 cattcaggaa aaggtgtcaa tgaaggaaat acttggggcg attattatta cttagaagct
     5101 ttgcttcgtt tttataaaga ttgggaatta tattggtagg aggttaaaat ggcaacacca
     5161 aatattgtaa tggctcgtat tgatgagcga ctaattcatg gacagggaca actatgggtt
     5221 aaatccttag gttgtaatac tgtcatcgtg gcaaatgata aggttagtga agataagatt
     5281 cagcagacat taatgaaaac ggttattcca gattcagtag ctatgcgttt cttctctttg
     5341 cagaaagtga ttgatgtgat tcataaggct aatcctgctc aaactatttt cttagttatt
     5401 aaagatttgc aagatgcact aactttggtt aaagggggag taccaatcac agaattaaat
     5461 attggaaata ttcataatgc gcctggaaaa acgcaagtaa cacggtctat cttttttggt
     5521 tcagaagata aggctatagt gagagagttg aacgatgttt ataagattcg cttcaacaca
     5581 aagacaacgc cttctggtaa tgatggggca gttgaagtta atatattgga ttatatttaa
     5641 aaaaataagg agaattatca tgacgatcag tctaattcaa gcagctctta ttggtctgtg
     5701 gacagctttc tgttttagcg gtctattatt ggggctttac acaaaccgat gtatcgtgct
     5761 ttcttttggg gtcggaatca ttttaggaga tctaccaacg gctttagcta tgggcgctat
     5821 cagtgagctg gcatacatgg gatttggtgt tggtgctggg gggaccgttc ctccaaatcc
     5881 aatcggacct ggtatttttg gtactttgat ggctattaca agtgctggaa aagtaacacc
     5941 agaagcagct ttagcacttt caacacctat tgcggtaggt attcaattct tacaaacttc
     6001 agcatatact ttacatgcgg gtgctccaga atcagctatc aaacatcttc gtgcaggaaa
     6061 cctttctaaa tttaaacgtg ttgcaaatgg tacagtgatt actttcgcct ttttaggatt
     6121 tgctcttggt ttcttgggtg cctattcaat ggatactctt ttgaaattgg ttgaaatgat
     6181 tccaccagta ttattaaccg gattgagtgt agcaggtaag atgcttccag ctatcggttt
     6241 tgcaatgatt ttgtctgtaa tggctaagaa agaattgatc ccgtttgtat tgcttggcta
     6301 tgtttgtgca gcttatctac aactgcctgt aattggtatc gcaattgtcg gtagtatctt
     6361 tgcacttaaa gaattcttta ataaaccaga acaagtggca gtgaaacagg aggaagcaca
     6421 cgatgactgg atctaaacta actaaaaaag attatctcaa cacttcttta cgggccttct
     6481 tcttgcagaa tggtttcaat tacagtaatt atcaagggat tggctatgca aatgtcatgt
     6541 atccagcttt taagaagcat tttggtgacg acaaagaagg attaactaaa gctttagaag
     6601 aaaactgtga gttttacaat accaatccac attttcttcc attcattact agtcttcatt
     6661 tggtcatgtt agaaaatgat ctcccagaag aagagactcg tgggattaag atggctctga
     6721 tggggccgtt ggcaggaatt ggtgactcac tttcacaatt tgtattagcc ccacttttat
     6781 ctacgattgc tgcctctcta gcaggtcaag gactggttat gggaccgatt cttttcttcc
     6841 ttggaatgaa cattatcttg acctctatca agttgttgac tggtttgtat ggctataaat
     6901 ttggtaccag cgtgattgat aagttgagtg accaaatggc aacaatttct aaggtagcca
     6961 atatcatcgg tgtgacagtt atctctggtt tagcagtaac ctttgtaaaa atttcggtgc
     7021 caatcacctt tgcggcgggt gaattaacag aaggagcaga tcagaaaatt gtaaccatcc
     7081 agggaatgtt agacaagatt gcccctgcgt tgcttccagc cttgtttacg attttggttt
     7141 attatctcat caagaacaag aaatggacaa cctacaaact agttatcctg acagttatca
     7201 tcggtatttt gggtagctgg ctgggcatcc ttgcataagc gcctatggaa caacaaactc
     7261 tctactatgt actaattatt ttagtagctc tatcgaccat acttcctgta ctcacttctt
     7321 atatcaatcg taagcgacaa caaaaggcac ttgaggagca actggaaaaa cgccaacgct
     7381 acttatcaac cttacaggct ggagatgagg ttatcctctt ctctgggctg catgggaaaa
     7441 tcatcaaact aacagacagc ttggtagaat tgcagattgc agaaggtgtt cttgtttacg
     7501 ttgaaaaaga gtcgattatg ggaaaagcca aagaactact ttttacgaag tagttcttct
     7561 ttcttgcctc cgcaaggaag cggttgcagg attaaaatta tacccctggg acattgtttt
     7621 gccccgcata aaaaggaaag gaaacaccaa tgggattttt tatcagtcaa agcaagcagc
     7681 attacggcat ccggaaatat aaggtgggtg tctgctccgc ccttatcgcg ctgtctatct
     7741 tgggaacaag ggttgcagcc aatcaactgc caagtgcaga aacagctagt ccacaaagca
     7801 gtcagttagt ggaaacaact ccagaaacga cagaagcagt aatgacaagc gaggtcagct
     7861 ctgaggttag tcctgttaca tcaacagaaa cccagccttc aagcaccact gcagaaactc
     7921 ttgccagtcc gcaagctgtt caggcgacaa aagaagagaa gaacctagta gccaatgggg
     7981 agttcatcag tacaacggca ccatcaggaa actggaaaga attggcagct acaaactggg
     8041 aaacatggat tcctgcaaat gtgaagaaag aaaacggaca ggttcgcatt gatgagggac
     8101 ggctgcatat ctcttcaaca gctagctatc gagtggcagt gcaccaaaca gtggatgtag
     8161 accctaacaa gcgctatctc tttagctatg atgtagaaac caaggacctg aaggggagcg
     8221 gtgttcgtgt tcgcttgcgc agtttgactg cagaaggcaa ggacttgagt ccacaagaat
     8281 ttgcctatac accttataaa aatggaagtc aggcggagca catagagcag atcttgacgg
     8341 tatctccgga aactcgtaaa ttgaaggtgg aactgttttt tgagaatagt gtcggccaag
     8401 cctggctgga taacatctcg ctggtcgaat acgtagaaaa gacaccagaa acaccagagc
     8461 aaagcccaga acttgtccaa cctgaaacag ggcaaataag tcttgccagc aacaaggtct
     8521 acctgcctgt tcggccagac ctgacctatc ggattgcgga tgcggctgtg gccacagtag
     8581 agaaaaatat gattcgtccg cttgcggctg gaaagactca ggtagatgtc tatgataagg
     8641 acaccaagct atctagtttc gagctgatag ttacagagca tcaggcaaca gtctttgata
     8701 ccttaaggaa caactgggaa gatatttctt tagccaataa acgctaccaa tctaacgatg
     8761 ctcagatgaa agcctttcta gggcgtttag atgccggtgt agcttcttct cttgaaaaat
     8821 gggttgaacc aacagagcag agcaaaacga ttttcaacga tattgacttt agtaaatcca
     8881 gccatctgac aacagtctat cgccgcttgg agcagatggc tcaagtagtt gagaatccag
     8941 actcagccta ttaccacgac cgctcactta tcgatttggt cagaaaagga atgaactggc
     9001 tttacaccaa tgtttataat gaaaataagt caattgatgg gaattggtgg gattatgaaa
     9061 ttggaactcc gcgtgcggtt gtcaacacct tgatctacat gcatccctac tttagtcagg
     9121 aggagattct aacctatacc aaaccgattt caaaatttgt accagatcca accactatcc
     9181 gtaaaacatt gaccaatcca gtcccagcag ttggcggtaa tcaaacggat ttgagcaagg
     9241 tggccatctt ggaaggagct cttcgcgaag atgctgaccg tgtgagggct ggtgcgcaag
     9301 gtttgacaac gattatgaaa tttgtagaca agggagaggg attctacagg gatggctcct
     9361 ttatcgacca caccaatgtt gcctacacag gggcgtacgg aaatgtcctc attgaaggtt
     9421 tttcacagtt actaccagtt attcagccaa cggagtttgc cctcaaggaa gaacagacca
     9481 acattcttta tgaatggatt gaaaaagcct ttatgccaat attggttcgc ggtgaactga
     9541 tggatatgac ccgcggtcgt tccatcagtc gggcaactgg tgaatcacat gttcaggcca
     9601 tggagatttt aaggtctttg gtccgcattg cagaatcagc tcagcctgag caaaaaacca
     9661 aacttctctc ctttgttaag gcacagctga cctctgatac cttctatgac agctaccgca
     9721 gcctcaaatc ctataaggat attgatttgg tcaacaagct tttagctgat aatcaaatcc
     9781 ctgctgaagt ggataaagat tatattgcag ccttcaacaa tatggataag tttgtttatc
     9841 gaagtgctca agaaggcttt acctttgcct tgtctatgta ctcttctaga actcaaaact
     9901 atgaagatat gaacaatgaa aaccgcaagg gttggtatac agcagatggc atggtttacc
     9961 tctacaatga tgatttgagt cattacagta accactactg ggcaacagtt gacccgtatc
    10021 gcttaccggg aacgacaacg accaaggaca aacgagaaga tggcagtggc gaggtaactc
    10081 ttgcaagtga ctttgttgga gcaagccagt taggcaaccg cttagcgacg attgcaatgg
    10141 atttcaacaa ctggaacaat agtctgactg ctcgtaaggc ttggattgtt ctgggaaata
    10201 agattgtttt cctaggtact gatatacagc atcaatctgc tcaaggcgcc gagacaacga
    10261 ttgaaaaccg taaactcttg accggagaaa aatatagcta ttacataaat ggccagccag
    10321 ttgatttgtc caaagaagta gtgacagata agactcaatc cttttacatg accaatggca
    10381 aggacaacca gtccatcggt tacgtcttct tgaaccaact gccgacccat gccaaattag
    10441 accagcgaac tggcaaatgg agtgacatca actacaacca gtcgaaagaa gaagtgagca
    10501 attcctttgt tagcctatgg catgaacatg cacagacttc aagtaactat gcttacgtcc
    10561 ttgttccaaa ccaatctatg gaaaaagtga atcaggcagc tgctagtgtg aaattgcttc
    10621 atcaagacag ggatttgcaa gtggtatacg accaagaaca gaatgtctgg ggcgttgtga
    10681 aatacacgga tacagcctac aagctgacag atgatattac cttaacagat gccggtttgt
    10741 ataccattca gaaagtcgaa ggtggctatc gcatcgcctt ttacaatccg tctacccgaa
    10801 ccgtaaagaa tggcattgag ttgacaaagg caggttctag tctgactgtt gagatggagc
    10861 cgactgcggc ttatccatcc actgtctgga aggttactat gccagaaggt tctgataaac
    10921 aaactggaag tgtcgaaaaa acagaaaaag aagaaaaaca actcaaggaa aaccaaccaa
    10981 gttcagaggt gaaacaagtg gttcatcatg ctgccgaaaa aacaaagccg agtaagcccc
    11041 gtctgcccca gacaggagaa gaagccagtc tgggtcttgg tttcttaggt ttgctaacct
    11101 tgggggcagt ggtggatttc aagtgtcgca gatcgcactc ctaaatgaaa ttcgtgggtg
    11161 aagggggagc gggaaagaac tcgactag
//
LOCUS       DAZQCJ010000061        11059 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000061 DAZQCJ010000000
VERSION     DAZQCJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11059
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.062"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            378..740
                     /locus_tag="ACHU66_001427"
     CDS             378..740
                     /locus_tag="ACHU66_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389704.1"
                     /translation="MSNQEYAPVKNAISEELKAVISKVYQLDHETGWTLHYLTDIMLN
                     HFHEDVARVPYGNLTPVEHSLNSITHRVESARVALLKAGYEDKIEVGNPMWYLKLALQ
                     DFEHMKKLAKKEAKKCKK"
     gene            728..925
                     /locus_tag="ACHU66_001428"
     CDS             728..925
                     /locus_tag="ACHU66_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389705.1"
                     /translation="MQEMTIETALTLIAIFTPLNIFLWLHLGTYQLHSKPKTKPEGKH
                     TRPLKNERYGAYIQSQGRYYN"
     gene            935..1120
                     /locus_tag="ACHU66_001429"
     CDS             935..1120
                     /locus_tag="ACHU66_001429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389706.1"
                     /translation="MKKLDQIVEEIKEQRPALYGLIIGIIENKIPKEEEERFLTLSQE
                     GKKQWIIDWTNLLEGQA"
     gene            1117..1455
                     /locus_tag="ACHU66_001430"
     CDS             1117..1455
                     /locus_tag="ACHU66_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389707.1"
                     /translation="MTENKLPEHLNKVFKLLPLGMDLPITAPDIEKLTGLDVRTIREH
                     IRQLIVDYGIPVCGGRDNKLGGYYIPQNEVERLAGVLPLQRQYDQEHKRIHALLTADL
                     QDWRKYRDEA"
     gene            1445..1729
                     /locus_tag="ACHU66_001431"
     CDS             1445..1729
                     /locus_tag="ACHU66_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFR3389708.1"
                     /translation="MKLELTAQSEADLKTGILELIENYLEAREQTPPRLLGLITAQQV
                     KDELGIKDKTLKRWEDNGLRRYQPPLEDTRKIFYRVSDILKFLGVENGKV"
     gene            1897..2367
                     /locus_tag="ACHU66_001432"
     CDS             1897..2367
                     /locus_tag="ACHU66_001432"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389709.1"
                     /translation="MKKLPITETQALKKGDFYKFYYFLPFDTYMTGFVYQGLMVTIVK
                     DYPEEVNGWELVRDRKIAMISNELLEKMGKLERHNLDKMRVNIDYIYTPMLTELLKYG
                     VNSRADVAYLVRELYINGMDMIGAIEIFANLTQRGDLAGYFLQVATSFFEGVTV"
     gene            2364..3962
                     /locus_tag="ACHU66_001433"
     CDS             2364..3962
                     /locus_tag="ACHU66_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HFR3389710.1"
                     /translation="MKQLKEILQLNWNEAEFGQSKLKADYKRQLYNIVAVDSLNNAWY
                     NGKTVFIPDNRIMYITEQNGTTKKYISATFEKKTKGKGDNKKEYIHITYDMNFSPFAK
                     LVTDYLLGRFVFYGGKLYDVNNKQAVLMDEITIQNLYGFKRDGEYVLEILSGIASNIK
                     IEPVRTLQPYQIAGNDFIIDLKEHRYYRTNPNEEQSYFKYYPVDYNTAKDGQAMANKF
                     LKYVIADELSLHNATLQTYYMAQVASGLRSKTNFFISKSGVRTGKGLRHIALSGLFNK
                     IDVELDNLISRGFDALNAWALFSGGEMALATEQGDIVGDRVERVLKIIATEKTHVARS
                     VGGNQGLVQLSSVLCIDTNRNVSLSDEMNGRKVLIQYQDRPEGETDAEREAVFAEYWQ
                     AFTHPDKSPKIDGCIGFLLTSLDYFMEQGQKFEWKAVEVFNDVDLDDFQMLLLNTLTE
                     QDYLVRTDNLFVEELYRKTYGNNGVKAKNAMETIGVGSKRKKIKNRLVTVYVVNNAKR
                     FTSYNPESEQPKELKLFSNIDDVL"
     gene            4302..4475
                     /locus_tag="ACHU66_001434"
     CDS             4302..4475
                     /locus_tag="ACHU66_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein Cse60"
                     /protein_id="HFR3389711.1"
                     /translation="MKIKLFYQRHSQFIKDFETEVNDFMSTVEVIDVKYTEATAGHFE
                     QLGTNTGLLVLYK"
     gene            4496..5071
                     /locus_tag="ACHU66_001435"
     CDS             4496..5071
                     /locus_tag="ACHU66_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389712.1"
                     /translation="MTLQTFSDTPKTFTFNYTLKDFDTAQVAGHALMGYMTGTFEQPA
                     IEVSYHNDNAGGDYNRLAVEYVADTELTETFQRICDSFQDYYNNPDELTGEDLDDYEQ
                     VQEIEQEYIRQRVEQLKQSESFDSLLEKVAGLELELMELADSVLDDDYPDMAVNGVWE
                     NMTAVDDEARKLLKELDTEDNYCALWKYSAE"
     gene            5121..5522
                     /locus_tag="ACHU66_001436"
     CDS             5121..5522
                     /locus_tag="ACHU66_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389713.1"
                     /translation="MMKNKGGRPTKMTQGTVKKLEEAFLRGLSDEEACLYANISKPTL
                     YDYCKKNPQFSDRKELLKQRLKTRAKLNISNAIEDGDVVISKWYLERKDDEFKTKTKL
                     EHDGNVSVSPHNPFEELTVEELRAIIAEDEG"
     gene            5690..6109
                     /locus_tag="ACHU66_001437"
     CDS             5690..6109
                     /locus_tag="ACHU66_001437"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389714.1"
                     /translation="MDLKQLAKFEKLVQSKKREIEALRAGIVNTPDYQSQRVTRSKKN
                     TTEDQVIKRIEKIEQLETELAQLYADYSGLSARIDAITDPVASLIMRLKYINGYSWLM
                     IKRTLPYYSQSSLYDYHKKGLEELEQTTSKKKTERVS"
     gene            6141..6461
                     /locus_tag="ACHU66_001438"
     CDS             6141..6461
                     /locus_tag="ACHU66_001438"
                     /inference="COORDINATES: protein motif:HMM:NF019021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFR3389715.1"
                     /translation="MIQVDENWIVKLFEFDPEDYKGRIYDWQREAPEEVNEIIKAVNA
                     IARPRHRAILIMSYISPEKIRSAEQAQQLGIKSSTYYLAKNKALEEFASQYRSGILER
                     YRGG"
     gene            complement(7008..8792)
                     /locus_tag="ACHU66_001439"
     CDS             complement(7008..8792)
                     /locus_tag="ACHU66_001439"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3389716.1"
                     /translation="MKTLRFFWFYFKRYKLSFAVIFLAIVAATYLQVKTPVFLGNAIA
                     EMGKIGQAYFMANQAGQADFQPDMADFNGVMLNLFFAYAATVVASLIYTLLFTRIVAH
                     STNRMRKGLFGKLERLTVAFFDSHKDGDILSRFTSDLDNIQNAFNQSLTQVVTNIALY
                     VGMVIMMFRQDTRLALVTIASTPVALIALVIIIRLSRKYTDKQQAAVSKLNAYMDEKI
                     SGQKAIIVQGVQEETIDGFLELNEEVRRTTFKGRLFGGILFPFMNGMSLVNTAIVIFA
                     GSSIVLNDSSLETAAALGLVVTFVQYSQQYYQPIMQVAASWAELQLAFTGAHRIQEMF
                     DEPEEVRPQNAPLFIELKEGVEIKDIDFGYLPGQKVLDKVSISAPKGKMVAVVGPTGS
                     GKTTIMNLINRFYDVNGGSVAFDGRDIREYDLDSLRDKVGIVLQESVLFSGTIADNIR
                     FGDESISQEMVETAARATHIHDFIMSLPEGYETFVTDDENIFSTGQKQLISIARTLLT
                     DPQVLILDEATSNVDTVTEAKIQKAMEAIIAGRTSFVIAHRLKTILNADEIIVLKDGK
                     VIEQGNHSQLLKLNGFYAELYHNQFVFE"
     gene            complement(8793..10499)
                     /locus_tag="ACHU66_001440"
     CDS             complement(8793..10499)
                     /locus_tag="ACHU66_001440"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3389717.1"
                     /translation="MFKEAILRYKWYALASVLLTSIVVATTLMQPSYLQDVLTAVLAN
                     DKEEIVRVGKFLLLIAGIGLLAGLTNTIMAAKISQGVSADIREKTFRKIQSFSYANVE
                     EFNAGNLVVRMTNDVNQIQNLVMMLFTILMRVPLLFVGAFIMAVRTLPELWWIIVLMV
                     IIIMGIMAVVMGLMGPRFGKFQSLMDRMNSIAKENLRGIRVVKSFVQEKNQYAKFKEV
                     SNELLDLNLFIGYGFSILQPLMMFISYMAIFVSLYLVSGMLDTNIEAVGGFTSFMSYL
                     MQIMFAIIMTSFMGMQASRAAISIKRLSEVLDTEPAMTFKDGAKEDLDGRIVFDNVSF
                     TYPHDTEPTLKNISFEIESGQMVGVVGATGAGKSTLAQLIPRLFDPQEGTVSIGGRDL
                     KDISQETLRDTVSIVLQKAILFSGTIADNLRQGAPGADLERLERAAGIAQAKEFIDRL
                     DDTYESQVEERGNNFSGGQKQRMSIARGVISDPKVLVLDDSTSALDAKSEKLVQEALN
                     HDLKGTTTVIVAQKISSVVKADKILVLDEGRLIGQGTHAELVATNDVYREIYETQKGR
                     EE"
     gene            complement(10492..10941)
                     /locus_tag="ACHU66_001441"
     CDS             complement(10492..10941)
                     /locus_tag="ACHU66_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFR3389718.1"
                     /translation="MGHTIADFRNLLNQIEQISETIAKEYDVEHLAGPQGWALRFIAE
                     RSEVETFVKDIEAELRISKSVASNLVKRMEKNDFIRVLPSQFDRRYKQLVLTEKGRSK
                     ICHLKSFHEEMHHSLFRGIQKEEFDLIRQVADQLKDNIQHYKEKNHV"
BASE COUNT         3648 a         2338 c         2363 g         2710 t
ORIGIN      
        1 atagctagat tatatcatat ctaggacact ttgaccatac agagggcgct aacccttaaa
       61 actggagtag aaaagtatta ggtgctggta tcgccattag cgaaagctaa accaccctaa
      121 gaaaatcaca cacagctagt ctatcatttg gcacaggctt acatgaccac gggaaaacct
      181 ggtaagtttg gggcggttat ccgctgggga tagtctaggc taacaaagaa atgtataaag
      241 aaaaaagtat agtaaagaaa tcaagccttt tacaaacagg ataaagccca ctagggtcat
      301 tacacagaca caataaaaga aacagaggta agaactacat gaaagacaat aacagagaaa
      361 cgataatcca ttttgaaatg tctaaccaag aatacgcacc cgtgaagaat gcaatatcgg
      421 aagagctgaa ggcggtcatc agtaaagtat atcaactaga ccatgaaaca ggctggacat
      481 tgcactatct aaccgacatc atgctgaacc actttcatga ggacgtggca cgagttccat
      541 atggcaattt gacaccggtt gaacacagtt tgaacagtat aactcaccga gtcgagagcg
      601 caagggtagc actcttgaaa gctggatatg aagacaagat tgaagtaggt aatcctatgt
      661 ggtacttaaa actagcctta caagattttg agcatatgaa aaaactggcc aagaaggagg
      721 ctaaaaaatg caagaaatga caattgagac agcgttaaca cttatagcaa tctttacacc
      781 gctaaacatc tttctatggc tacatctagg aacatatcag ctccacagca aaccgaaaac
      841 caaaccagag ggtaaacata ccagacctct taaaaatgag cgctacgggg cttatattca
      901 atcacaaggc agatattaca attaggaggt ggacatgaag aaactagacc agatagttga
      961 ggaaatcaag gagcaacgcc cagcactcta tgggcttatc attggcatca tagaaaacaa
     1021 gatacctaaa gaggaagagg aacgtttttt gacactatcc caagaaggaa aaaagcaatg
     1081 gatcattgac tggacgaatc tgctggaggg acaggcatga cagaaaacaa attaccagaa
     1141 catctaaata aagttttcaa gctcctaccg cttggaatgg atttgccaat caccgcccca
     1201 gatattgaga agctgacagg cttggacgtc cgaaccatta gggaacatat ccgccagcta
     1261 atagttgact acggtattcc cgtttgtggt ggacgagata acaagctagg gggctactat
     1321 atcccccaga atgaagtaga acgacttgca ggagtgctac cgcttcaacg acaatatgac
     1381 caagaacaca agcgtattca cgcgctactg accgcagact tgcaagactg gaggaagtac
     1441 agagatgaag cttgaattaa ccgcacagag tgaagcagac ctaaaaacgg gcattctgga
     1501 gcttatagag aactatctgg aagcgcgtga gcaaacaccg ccaagactgt tagggctaat
     1561 cacagcccaa caggttaagg atgaactagg cataaaggat aagaccttga aacgctggga
     1621 agacaacggg ctaagacgtt accagccccc actagaagac actaggaaaa tcttttatag
     1681 ggtcagtgat attctgaaat ttttgggggt ggaaaatggc aaggtatagc atacacccag
     1741 cagacagtgg cggacactat cacgatatta aattatacaa agaccgcagg gcgaccctgg
     1801 accagctaaa ggaacagcaa cgtttgaaga aactcaaaaa gaaacggaga aagtgacatg
     1861 tatagcctaa ttgagatcac aactaacaag gagaacatga aaaagttacc tatcacagaa
     1921 acccaagcac tcaagaaagg ggatttctac aagttctact actttttgcc ctttgatacc
     1981 tatatgactg gtttcgtcta tcaagggcta atggtgacaa tcgtcaaaga ctaccccgaa
     2041 gaagtaaacg gttgggagtt ggtcagagat agaaaaatag ctatgataag caatgaacta
     2101 ctagagaaga tgggaaagct ggaacggcac aacttggata agatgcgggt caacatcgat
     2161 tatatttaca cgccaatgtt gacggagcta ctgaaatacg gcgttaactc acgggctgac
     2221 gtggcttact tggtcagaga gctttatatc aacggtatgg acatgattgg cgcaattgaa
     2281 atctttgcca atctcaccca acggggcgac ctagcaggct atttcttgca agtggcaaca
     2341 tcattttttg agggggtgac tgtttgaaac aattaaaaga gattttacaa ctgaattgga
     2401 atgaggcgga gtttggacag tctaaactaa aagcagacta caagagacaa ctgtataaca
     2461 ttgtggcagt tgatagcttg aataatgcct ggtacaacgg aaaaactgta ttcattcctg
     2521 ataacaggat catgtacatc accgagcaga atggcacaac aaaaaaatac atatccgcaa
     2581 catttgagaa aaagaccaaa gggaaaggag acaacaaaaa ggagtatatt catatcactt
     2641 acgacatgaa tttctcaccc tttgctaagt tggtaactga ttatctgtta gggcggttcg
     2701 tgttctatgg tggtaaatta tacgacgtaa acaacaagca agctgttcta atggatgaaa
     2761 tcaccataca gaacttatat ggcttcaagc gtgatgggga gtacgttctg gaaatccttt
     2821 caggtatcgc cagcaatatc aaaattgagc cagtacgaac gctacagccc tatcagatag
     2881 cagggaatga ctttattatt gacttgaaag aacaccgcta ctacaggact aacccaaatg
     2941 aagaacaatc ctattttaag tattacccag tggactacaa caccgccaaa gatggacagg
     3001 ctatggctaa caagtttcta aagtatgtaa tagctgatga actttcatta cacaatgcga
     3061 ctttacaaac ctactacatg gcccaggttg ccagcggttt acgatcaaag accaatttct
     3121 tcatttcaaa atctggagta cgaacaggaa aaggcttgcg acacatagcg ttgtctggac
     3181 tatttaacaa gatagatgtg gaattagaca atcttatctc aagaggattt gatgcactca
     3241 atgcgtgggc tttgttttca ggcggggaga tggctttggc aacggagcaa ggcgacatag
     3301 taggcgacag ggtggagagg gtgctgaaga ttatcgcaac cgaaaaaaca cacgtcgccc
     3361 gaagcgtcgg aggaaaccag gggcttgtac agttgtctag cgtgctatgt atcgatacca
     3421 atagaaatgt atcgctatcc gatgaaatga acggacgcaa ggtcctcata cagtaccagg
     3481 acagaccaga gggcgaaaca gacgcagaac gtgaagccgt cttcgcggaa tattggcagg
     3541 cattcactca ccccgataag tctcctaaga tagatggctg tattggtttc ttgctgacta
     3601 gcttggacta tttcatggag caagggcaaa aattcgaatg gaaagccgtt gaagttttta
     3661 atgatgtgga tctggatgac tttcaaatgt tgctactgaa tacattgact gaacaggatt
     3721 atcttgtaag gactgataat ctatttgttg aagagcttta tagaaagacc tacggcaaca
     3781 acggagtcaa agcaaagaat gcaatggaaa ccatcggagt ggggtcaaag cgtaaaaaaa
     3841 taaaaaatag gttagtcact gtttacgttg tgaacaacgc taaacgcttc acaagttaca
     3901 accctgaaag tgaacaacct aaagaattga agttatttag caacattgat gacgtgcttt
     3961 gaggtatcaa ggggtacatg ggggttacat agcttttaag caaacccccg atacctcaca
     4021 aacccagtta taccaatact ttatctacta ttatattact tagtacatag gtacacatag
     4081 gtatatatag gataaaataa aaaaagataa taattagaca aaaaatacat atacaaatat
     4141 agtgcaaaaa aacaaaaaaa tagtaaatat atatagtaaa atccctgata ccttatgaac
     4201 cactgtaaaa actcagtaaa atcaagggtt tgagtggggt cacgggtttc acatacccct
     4261 gaaaaagtat cacaggtcta gccaaaaaat ggaggaaaaa catgaaaatt aaactatttt
     4321 atcaaaggca tagccaattc ataaaggatt ttgaaacgga agtaaatgac ttcatgtcaa
     4381 ctgttgaagt gattgacgtg aaatatactg aagcaaccgc aggacatttt gagcaattag
     4441 gaacgaacac aggcctattg gtcttgtaca aataacagaa acggagaata acaccatgac
     4501 attacaaaca ttttcagaca ccccaaaaac attcacattc aattacacac tcaaagactt
     4561 tgataccgca caagttgcag gtcacgcgct tatgggctat atgacaggaa catttgaaca
     4621 accagctatc gaagtgagct accacaatga caacgcaggc ggagattaca accgtttggc
     4681 ggtggaatat gtggcagata ctgaacttac tgaaacattc cagcggattt gtgacagttt
     4741 ccaagattac tacaacaatc ctgatgagtt aacaggcgaa gacttggacg actacgaaca
     4801 agtacaggaa atcgaacaag agtatatccg tcagcgcgtg gaacaactca agcagtcaga
     4861 gagctttgac agcttacttg aaaaagtggc tgggttagag cttgaactga tggagctagc
     4921 tgacagcgtg ctagacgatg attatcctga tatggcagtg aatggggtat gggagaatat
     4981 gacggccgta gatgatgaag caagaaagtt actgaaggag ctggataccg aagataacta
     5041 ttgcgctttg tggaagtatt cagcagaata gcagagagag gcaactgcct ctttttgtgc
     5101 taaaattaga gaggaataac atgatgaaaa ataaaggcgg tagacctaca aaaatgacac
     5161 aaggaacggt aaagaaatta gaggaagcat ttctaagagg gctaagcgat gaagaagctt
     5221 gtttgtatgc gaatatttca aaaccaaccc tgtatgatta ttgcaagaaa aacccacagt
     5281 tttctgaccg aaaagagcta cttaagcaac gactgaaaac acgcgcaaag ttgaacatat
     5341 ctaacgccat agaggatgga gacgtggtta tttccaaatg gtaccttgag cggaaagatg
     5401 atgaatttaa aaccaagaca aaactagaac atgatggcaa tgtgtctgta tcgcctcaca
     5461 atccatttga agaattgacg gttgaagagt tacgagcaat cattgctgaa gatgaaggat
     5521 aaatactatt ggttgagagg gttatctagg tgaagtactt cggcaatttc taaaacgacg
     5581 aatagacgaa cgtatggaag cgcgtgatta cacaaaaaaa gccagcacta cttgtaccga
     5641 ctctgtgaat aaaactctaa aactattata tcacacggag gtgcgtagcg tggatctaaa
     5701 acagttggct aagtttgaaa agctggtaca gtcaaagaaa cgagagatag aagccttgag
     5761 ggctggcatt gtcaatacac cagactatca aagtcagagg gtaacacgat ccaagaaaaa
     5821 cactacagaa gaccaggtta taaaacggat tgagaagatt gagcaactag aaactgaact
     5881 agcacagttg tatgctgatt acagtggact atccgcccgc atcgatgcaa tcaccgaccc
     5941 agttgcaagc ctgataatgc ggttgaaata cataaacggc tattcttggt taatgattaa
     6001 gcggacattg ccatactact cccagagtag tctatacgat tatcacaaga aaggactgga
     6061 agagcttgag caaacaacta gcaaaaagaa aactgaaaga gtttcataga tggtgcaggg
     6121 tggcagttct gcattatgac atgatacagg tagatgagaa ttggattgtg aaactatttg
     6181 aatttgaccc cgaagactac aagggcagga tatatgactg gcaacgtgaa gcccctgaag
     6241 aagtgaatga gattataaag gcagtcaatg caatagctag accaagacat cgagcaatac
     6301 ttatcatgag ttatatatca cccgaaaaga tacgatcagc agagcaggcg caacaacttg
     6361 gtattaagtc atctacttac tacttggcca agaataaagc cttggaagag tttgccagtc
     6421 agtaccgtag cgggatacta gaacgatata ggggcgggta accctaccta ccctacttac
     6481 cgtacttcac gccgtcattg cgcaaatttt ttcgcgggaa atatgctatt cttcaatttt
     6541 gcctattttg agcaaatagc taccacgcgc catagtctat ttcacaaaaa tggtaatttt
     6601 gaacggatac agtaaaacac agtaggatac agtaaaaaca tgatataata cacttatcag
     6661 caatcaaaaa agcgtagctg atcactacgc tggtcctgcc tgctgaactc atataatttt
     6721 taggttgtca aacgttaatt tgataacctt tttcattgta aaatttgttc accttcttgt
     6781 tcacctttgg aggtgttcta aggaaatcta aagagatacg attttttaaa aacccagtaa
     6841 aatcacgagt ttaggagtta gaacactctc aaacgttagt caaatttcaa ttccacggat
     6901 tttcatagta gtttcccttc aatttcttcc tgcataatac gctctctatt ataatttatt
     6961 cgtaggctaa aagcaaaact ccaacctagt ggctggggtt ttcaacttta ctcaaacaca
     7021 aactggttgt ggtagagttc ggcatagaag ccgttgagct tgaggagttg gctgtggttg
     7081 ccttgctcga tcacttttcc gtctttgagg acgatgattt cgtccgcatt gaggatggtt
     7141 ttgaggcggt gggcaatgac gaagctggtc cgtccagcga taatagcctc catagccttt
     7201 tgaattttgg cttccgtcac cgtatcaacg tttgaggttg cttcgtccaa aatcaagact
     7261 tgtggatctg tcaagagcgt acgggcaatg gaaatcagct gtttctgacc tgtcgagaag
     7321 atattctcat catcagtcac aaaggtttca taaccctctg gcaagctcat gataaagtcg
     7381 tggatatggg tggcacgagc tgcggtttca accatttcct gcgaaatgct ctcatcacca
     7441 aagcgaatat tgtccgcaat agtacctgag aaaagaaccg actcctgcaa gacaatgccg
     7501 accttatccc gcaagctgtc caagtcatat tcccgaatat cacgaccgtc aaaagccaca
     7561 ctaccaccat tgacatcgta gaagcggtta atcaagttca taatggtagt cttaccagat
     7621 cccgtcggac caacgactgc caccattttg cccttaggag cagagattga caccttgtcc
     7681 aagaccttct gacccggcag gtagccaaag tcaatgtcct tgatttcaac accttctttt
     7741 aattcgataa atagcggagc gttttgaggg cgaacttcct caggctcatc aaacatttcc
     7801 tgaatacgat gagctcctgt gaaagctagc tgcaattctg cccagctggc agcaacctgc
     7861 atgattggct ggtaatactg ttgcgaatat tgaacaaaag tcaccaccag accaagtgcg
     7921 gcagctgttt ctagcgaact atcgttgaga acaatgctgg aacctgcaaa gataacaatg
     7981 gcagtattga ccaaactcat cccattcata aatgggaaga gaatcccacc aaacaagcgt
     8041 cccttgaagg tcgtacgacg aacttcttca ttgagttcca agaaaccatc aattgtctct
     8101 tcctgcacac cttgtacaat aatcgctttt tgtccggaaa tcttctcatc catgtatgca
     8161 ttaagcttag ataccgcagc ctgttgctta tcagtatatt tccgtgacag gcgaatgata
     8221 atcactaggg caatcaaagc aactggcgta gaagcaatgg tcaccaaggc caagcgagta
     8281 tcctgacgga acatcatgat gaccatacca acataaagag cgatgttggt caccacttgg
     8341 gtcaaggact ggttaaaagc gttttggata ttgtccaaat cactggtaaa gcgagaaaga
     8401 atatccccgt ccttgtggct atcaaagaag gcaactgtca agcgttccag cttgccaaac
     8461 aagcccttac gcatacggtt ggtcgaatga gccacgatac gcgtgaagag gagggtgtaa
     8521 atcaaggaag ccacaaccgt cgccgcatag gcaaagaaaa gattgagcat aaccccgtta
     8581 aaatcagcca tatctggctg aaagtcagcc tgaccagctt gattggccat aaagtaagcc
     8641 tgcccaattt tccccatctc cgcaatggca tttccaagga aaacaggtgt cttgacctgc
     8701 aagtaggtcg ctgccacaat agctagaaaa atcacagcaa aggacagttt atagcgttta
     8761 aaataaaacc agaaaaaacg taatgtcttc atctattcct ccctcccttt ctgtgtttcg
     8821 tagatttcac ggtaaacatc gttcgtcgct accaactccg cgtgggtacc ttgaccgatc
     8881 agacggcctt cgtcaagaac caaaatctta tcagctttga caacagaaga aatcttctga
     8941 gcaacgatga ccgtcgttgt ccccttcaag tcatggttga gggcctcttg gaccagcttc
     9001 tcagacttgg catccaaggc tgatgtcgaa tcatctagca ccaagacttt aggatcacta
     9061 atcaccccac gcgcaatgga catccgctgt ttctgaccac ctgagaagtt atttccacgt
     9121 tcttctactt ggctctcata ggtatcatcc aagcggtcga taaattcttt ggcttgggca
     9181 atacctgcgg cccgctccaa acgttccaaa tctgcaccag gtgcaccttg acgcaggttg
     9241 tctgcaatgg tccctgagaa gagaatagct ttttgcaaaa cgatagacac tgtatctctc
     9301 aaggtttcct gactgatgtc tttcaaatca cgaccgccaa ttgacacagt cccttcctgt
     9361 ggatcaaaca aacgcggaat gagctgagct agtgttgatt tacccgcacc cgttgcaccg
     9421 accacaccga ccatctgacc agactcaatc tcaaaggaga tatttttcaa ggtcggctcg
     9481 gtatcatgcg gataggtgaa gctaacattg tcaaagacaa tccgaccatc caagtcctct
     9541 tttgcaccat ctttaaaggt catcgctggc tctgtatcta aaacctcact caaacgttta
     9601 atggaaatag cagcacgtga agcctgcatc cccataaagc tagtcatgat aatggcaaac
     9661 atgatttgca tgaggtaact cataaaggaa gtaaagcccc caactgcttc gatattagtg
     9721 tctaacatcc ctgaaacgag ataaagcgac acaaaaatcg ccatgtagga gataaacatc
     9781 atcaaaggtt gcaagataga aaaaccataa ccaataaaga ggttaagatc caaaagctca
     9841 ttggatactt ctttaaattt agcgtattga ttcttctcct gtacaaagga cttaaccaca
     9901 cgaatcccac ggaggttttc ctttgcaatg ctgttcatcc tgtccatcag ggactggaat
     9961 ttaccaaagc gcggtcccat caagcccatc acgactgcca tgattcccat gatgatgatg
    10021 accatgagca cgataatcca ccaaagttct ggcaaagtgc gcaccgccat gataaaggca
    10081 ccaacaaaga gcagaggtac ccgcatcaag atcgtaaaga gcatcatgac aaggttctga
    10141 atttggttga cgtcgttggt catacgaacc accaaattcc ctgcgttaaa ttcctctaca
    10201 ttggcataag aaaagctctg aattttccga aaggtcttct cacgaatatc cgcagacact
    10261 ccctgagaaa tcttagctgc catgattgta ttggtcagac cagccaagag accgattccc
    10321 gcaatcagca agaggaactt accgacacga acaatttctt ccttatcatt ggccaagaca
    10381 gcagtcaata catcctgtaa ataactaggt tgcatcaagg tcgttgctac gacaatagac
    10441 gtcaacaaga ccgatgccaa ggcataccat ttgtaacgta aaatggcttc tttaaacatg
    10501 attcttctcc ttatagtgtt gaatattatc ttttaattga tcggctacct gtctgatcaa
    10561 atcaaattct tccttttgaa tacctcgaaa gagagaatgg tgcatctcct catggaagga
    10621 ttttaggtga caaatcttgc tccgaccttt ctctgtcaag accaactgct tatagcgtct
    10681 gtcaaactga gaaggtaaaa cccgaataaa gtcatttttc tccattcgct taactagatt
    10741 gctggcaacg gatttggaaa tcctcaactc cgcttcaatg tctttgacaa aggtttcaac
    10801 ttcagaccgt tctgctatga aacgcagagc ccatccctgt ggtccagcta ggtgctccac
    10861 atcatattcc tttgcaatgg tttcactgat ttgttcaatc tgatttaata gattccgaaa
    10921 atctgcaata gtatgtccca taatagctcc tttctacgaa aataattacc atgagaacta
    10981 ttatagttct cttaggaact attgtcaatt attccgtttg ctcttttctg aaaatatttt
    11041 gttatttcag caccattac
//
LOCUS       DAZQCJ010000062        10594 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000062 DAZQCJ010000000
VERSION     DAZQCJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10594
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.063"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(110..1546)
                     /locus_tag="ACHU66_001442"
     CDS             complement(110..1546)
                     /locus_tag="ACHU66_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFR3389719.1"
                     /translation="MKELYNTLYELGTIWREEHQYTVNEGKKNEKIVIPIPEVSTVAK
                     YLRQVCHFAFISTGDSSDKSPLYLYHYDKGVYTDSVDLFNKLCAKFDSRLKPRAWADI
                     RAFIRTTTRISKPFSDHTRIPVANGVFNLKTKQLEPFSPDFVITSKIRTAYNGAARKP
                     ILDGWFDFEQWLDIIACGDSEIYALLWQIMNEAINPNRTRGKLAILLGDGNNGKGTFQ
                     SLLMNLIGADKVATLRPDQFEGHNLASLSGKVCNIGDDISNRYIDEVSDLMSIVTGDT
                     VTINPKHQQPFELSLKLFCLFSANELPRVRNKSQGWYRRLCIVPFKADFNGQKERPEI
                     KNIFLKDTELLEWVLFKILNMPAFDKFIEPEAVTKEIDSYKKENDYLYAFVTDDYTER
                     ELHLIERVPLKWIKEEYRTFLAENDLSAHIPYSFGKDLVRILRVHTGGKYSLRQGRLK
                     KKEAEVFPYPLTIAEYTEYPLRLVEKDE"
     gene            complement(1609..2487)
                     /locus_tag="ACHU66_001443"
     CDS             complement(1609..2487)
                     /locus_tag="ACHU66_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HFR3389720.1"
                     /translation="MAIYEARGFQNNLVYPFDRLEPFEYIAQFRPMKVPEGVDIEQFK
                     RTQAPYCISGKVTADKKGTHRRNNSSLVYRDLIFLDYDELEASVDFPRIVSEALSEYS
                     YIIFPTIKHTAKKPRYRLVVKPSHKMKETTYRATVQEIADKIGLPFDIASLTWSQLQG
                     LPVTTGDPEEYQKIIHRGSDYPIQAVLDKQPTKKAVTTAYTPRPSGHRSITMRVIDTL
                     FNGFGDEGGRNVAVTRFVGLLVGKWVNCDIPTAWELTKIANSVTAEPLPDSELEATFE
                     SIVKTEIRKRGLGISN"
     gene            complement(2622..2906)
                     /locus_tag="ACHU66_001444"
     CDS             complement(2622..2906)
                     /locus_tag="ACHU66_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFR3389721.1"
                     /translation="MRLELTAQSETDLKTGILELIGNYLEAREMVKPRVTGLITAQQV
                     KDELDIEYKTLKRWEDNGLRRYQPPIEDTKKIFYRISDILIFLGVENGKV"
     gene            complement(2896..3234)
                     /locus_tag="ACHU66_001445"
     CDS             complement(2896..3234)
                     /locus_tag="ACHU66_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389722.1"
                     /translation="MTENRLPPHLYKVFKLLPLGMELPITGTDIERLTGLDIRTIREH
                     IRQLIVDYGIPVCGGRDNKLGGYYIPQNETERLAGVLPLQRQYDQEHKRIHALLTADL
                     QDWRKYRDEA"
     gene            complement(3231..3380)
                     /locus_tag="ACHU66_001446"
     CDS             complement(3231..3380)
                     /locus_tag="ACHU66_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389723.1"
                     /translation="MLTFRELEHIAETILKHTTPEKMQCYLDMEHDSKLLWIKYKIAS
                     LEVQV"
     gene            complement(3392..3595)
                     /locus_tag="ACHU66_001447"
     CDS             complement(3392..3595)
                     /locus_tag="ACHU66_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389724.1"
                     /translation="MQEMTIETALTLIAIFTPLNLYLWFGVGLGTFRLDIEPKIKTEG
                     KYTRPLKNERYGAYIQLAGKRYN"
     gene            complement(3583..3909)
                     /locus_tag="ACHU66_001448"
     CDS             complement(3583..3909)
                     /locus_tag="ACHU66_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389725.1"
                     /translation="MKNAISEELKAVISKVYQLDHETGWTLHYLTDIMLNHFHEDVIR
                     VPYGDLTPIEHSLNGITHRVESARVALLKAGYEDKFEVGNPMWYLKLILQDLEHMKKE
                     IKKCKK"
     gene            complement(4420..5043)
                     /locus_tag="ACHU66_001449"
     CDS             complement(4420..5043)
                     /locus_tag="ACHU66_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR3389726.1"
                     /translation="MELVYMDGRKEPYTTSEIIAECAGVQHHTVTRLLRNHKERFEAF
                     GFYGFEIHKLDGKGRPKKVYHLNEQQATLLITYLDNTPQVVKFKTNLVRAFFEMRDEV
                     AQFRYQRALEKPKRKALHEAIETWQEAPKHAHSTVTNLLLKGTTGMNKRQLVAHRGGF
                     NGIDSLTSQELIRYQALEDMAIAMINLGMTYQEIKNMVFRKNAPQGA"
     gene            complement(5047..5793)
                     /locus_tag="ACHU66_001450"
     CDS             complement(5047..5793)
                     /locus_tag="ACHU66_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HFR3389727.1"
                     /translation="MRRNYSKVIEEMRTIHGLNLVAIGQRIGTDPRTVGKWAQGKHQP
                     NKDSRKKINNLYREVKQSMTTQTTIEPFEDFYDWSKMKKKKPTTEVIATQHFGNKSFE
                     IYGNKDNPLFIAVEVAEMIEVQNTTDLLKRIDEDEKLTYVISRAGQKREVNMLTEFGL
                     YEVLFQSRKPKAKEFKKVVKNILKEIRVNGYYMQGELIQDQPTTQPLPAISDLTYIKN
                     KLAEVQEMDNLADITAGIERVTKLVAVIGG"
     gene            complement(5812..6009)
                     /locus_tag="ACHU66_001451"
     CDS             complement(5812..6009)
                     /locus_tag="ACHU66_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR3389728.1"
                     /translation="MKNNMRVLLAKRREKVSHVHEATGISKSTLTKLYYERTKNPDIT
                     VLQKVANYLGVTIDELLTPEE"
     gene            6199..6780
                     /locus_tag="ACHU66_001452"
     CDS             6199..6780
                     /locus_tag="ACHU66_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR3389729.1"
                     /translation="MNRLKILRKEKGLSQQALAKEIGVSYRTLQNWENGVNSIKPDKA
                     QALADHFGVSVGYLLGFSSESSKNVLTEFKEGEYSQIEEDRAKIGNDKLILYSTITDT
                     RQFLDRMQRNIDMDAIIRKLPLPELLKLKNPKEREKFARKQMEPFIREMKNLAIALSY
                     LTDEESELLARFYVLNKQDKDRLLQIARTFTED"
     gene            7597..8763
                     /locus_tag="ACHU66_001453"
     CDS             7597..8763
                     /locus_tag="ACHU66_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR3389730.1"
                     /translation="MKITEVKKKNGATVYRASVYLGVDQVTGKKVKTKVTGRTKKEVK
                     NKTQQAIATFKTDGATRYQNATITSYKELAELWWNSYKHTVKPNTRGNIRGLLKNHVI
                     PLFGAYKLDKLTTPLIQSIVIKLADKANTGEAGAYLHYDKIHALNKRILQYGVVMQVL
                     PYNPAREVILPRNAKKATRKKVKHFNDEQLKQFLDYLDGLDLTNYRNLYEATLYKFLL
                     ATGCRINEALALSWSDIDLNNATIYITKTLNRYGSINSPKSNASIRDIDIDSQTVAML
                     KEYRWRQIQEAWTLGRSETVVFSDFIHDYPEDKTLGNRLTTRLKNIGLPNIGFHGFRH
                     THASLLLNSGIPYKELQHRLGHSRISMTMDIYSHLSKENAKHAVTFYEKALENL"
     gene            8901..9284
                     /gene="yaaA"
                     /locus_tag="ACHU66_001454"
     CDS             8901..9284
                     /gene="yaaA"
                     /locus_tag="ACHU66_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFR3389731.1"
                     /translation="MEFKLFDDYITLQALLKTTGILHSGGAIKGFLEENTILFNGEDE
                     KRRGKKIRVGDVITLPDHNTSITIVAPSAEEIKQHQEDQAEKERVAAIVKKLNQENKK
                     NKKQVKTPKKATKNTERKPVRFPGM"
     gene            9287..10381
                     /gene="recF"
                     /locus_tag="ACHU66_001455"
     CDS             9287..10381
                     /gene="recF"
                     /locus_tag="ACHU66_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR3389732.1"
                     /translation="MWLERLELQHFRNYNQLDIEFHKGLNVFLGENAQGKTNILESIY
                     VLALTRSHRTRTDKDLLQFQEKELSISGLLHRTSGKVPLDIHLTDKGRVTKVNHLKQA
                     KLSNYIGHMNVVLFAPEDLQLIKGAPALRRKFIDVELGQIKPLYLSDLSNYNHVLKQR
                     NTYLKSTDKIDENFLSVLDQQLAEYGSRVIQHRIDFLKKLEEFGNRKVQEISGNREEL
                     TIEYQTSIKLTDDVNLIDKFLTELERCRKRDLFKKNTGVGPHRDDVAFFINGMNAHYG
                     SQGQHRSLVLSLKLAEIELMKEVTREYPILLLDDVMSELDNNRQIKLLETITDTIQTF
                     ITTTSLDHLHKLPDSLKIFHIESGKVTESE"
BASE COUNT         2980 a         2268 c         2071 g         3275 t
ORIGIN      
        1 gactacaacg actacaaaca acacgtaaac ccttgatatg attgaataaa ttctgtagtc
       61 gttctccaaa aaaacaacga ctacagaacg actacaacga ctacaaaatc tactcatctt
      121 tttcaaccaa ccgtaaaggg tattcggtgt actctgcaat ggtcagcgga tagggaaaaa
      181 cttcagcttc ttttttcttc aatctgcctt gtctgagtga atacttaccg cctgtgtgta
      241 ccctcaagat tcttacaagg tctttcccga aactgtacgg aatatgtgcc gataggtcat
      301 tctcagctaa aaaagtccga tattcttctt ttatccattt caggggaaca cgctctatca
      361 aatgtaattc ccgttcggta tagtcgtccg tcacaaatgc gtataagtag tcattttctt
      421 tcttgtagct gtcaatctct ttggtcactg cttcgggttc aataaacttg tcaaacgctg
      481 gcatattcaa gattttaaat agtacccact ctaacagctc tgtatctttc aagaatatgt
      541 ttttaatctc tgggcgttct ttctggccat taaaatccgc tttgaacggt acaatacaaa
      601 gccgtctata ccagccttgt gacttgttcc gaactcttgg caactcgtta gcagaaaaca
      661 aacagaatag ttttaaactc aactcaaagg gttgttggtg tttcgggtta attgtcacgg
      721 tgtcgcctgt cacaatactc atcagatcag aaacttcatc gatgtatctg ttagaaatat
      781 catcaccgat attacaaact ttacccgata gactggctaa gttgtgcccc tcgaattggt
      841 cggggcgtaa ggttgccacc ttgtccgctc ctatcaggtt catcagtagg ctctgaaatg
      901 tgcccttacc gttgtttccg tcacctaata gaattgcaag ttttccccgt gtcctatttg
      961 ggttgatagc ttcattcatg atctgccaca ataaagcata tatttcactg tcaccgcatg
     1021 caataatgtc tagccattgt tcaaagtcaa accagccatc tagtattggt tttcttgctg
     1081 ctccattgta tgccgttcta atcttacttg tgataacaaa atcagggcta aatggttcta
     1141 attgctttgt cttgagatta aacaccccgt ttgctacggg tatgcgtgtg tggtcgctga
     1201 aaggtttgga aattcgggta gttgtccgaa tgaacgcccg aatgtctgcc catgctctag
     1261 gttttaacct gctgtcaaac ttagcacata gtttgttgaa taagtccacg ctatcagtat
     1321 atacaccctt gtcgtagtga tacaagtaca gcggtgactt gtccgaactg tcacccgtgg
     1381 agatgaaagc aaagtgacaa acctgtcgca aatacttagc tacggtacta acttctggga
     1441 ttggaattac tattttttca ttcttcttgc cctcgttgac tgtgtactgg tgttcttctc
     1501 tccagattgt accaagttcg tataatgtgt tataaagttc cttcatgctt gtaggctctg
     1561 aaaactctgc ccatgctgtc gcatggtcaa gttctgcctg taattgttct agttcgatat
     1621 tcctaaccct cgctttctaa tttctgtttt tacaatactt tcaaacgttg cttccagttc
     1681 gctatccggc aatggttcag ccgtcacgct gttggctatc ttggtaagct cccacgctgt
     1741 cggtatatca cagttgaccc atttgccaac caataagccc acaaatcggg taactgccac
     1801 attcctaccg ccttcatctc caaaaccatt gaaaagcgta tctataaccc tcatagtgat
     1861 tgaacggtgc ccgctaggtc gtggggtgta agcagtagtt acagcctttt ttgtcggctg
     1921 tttatccaat accgcttgaa tagggtaatc gctccccctg tggataattt tctgatattc
     1981 ttctggatct ccagtcgtca cgggtaagcc ctgcaactgt gaccaggtta aactagctat
     2041 atcaaagggt agtccgattt tatccgctat ctcttgcact gttgccctgt aggtcgtttc
     2101 tttcatcttg tgactaggct tcactacaag cctataacgg ggctttttag ccgtgtgttt
     2161 gattgtcggg aaaatgatat aggagtattc agaaagtgcc tcagaaacga ttctgggaaa
     2221 gtctacgctt gcttctagct cgtcatagtc caagaaaatc aaatcacgat agactaggct
     2281 tgaattattc cgcctatgag tgcctttttt atctgctgtg actttgccac tgatacagta
     2341 gggggcttgt gtgcgtttga attgttcaat gtccacaccc tcaggtactt tcataggtct
     2401 aaactgtgcg atatactcaa acggttctaa cctatcaaac ggatagacta gattattctg
     2461 aaagccccta gcctcgtaaa ttgccacatt atcgcccctt tctccgtttc ttcttcagct
     2521 ttttaagcct ttgctgttcc tttagctggt ccagggttgg gcgacgatct ttataaagtt
     2581 taatatcgtg gtagtgtccg ccgctgtctg ctgggtgtat gctatacctt gccattctcc
     2641 acccccaaaa atatcaaaat atcactaatc ctataaaata ttttcttagt gtcctctatc
     2701 ggtggttggt atcgtctcag cccgttatcc tcccaccgct tcaaagtctt gtattctatg
     2761 tctagttcat ctttgacttg ctgggctgta attagccctg taacccttgg cttgaccatc
     2821 tcacgcgcct ccagatagtt ccctataagc tccagaatgc ccgtttttag gtccgtttca
     2881 ctttgtgcgg ttagttcaag cctcatctct gtacttcctc cagtcttgta aatcagcggt
     2941 aagaagcgcg tggatacgct tatgctcttg gtcgtattgc cgttgtagtg gtagcactcc
     3001 tgcaagccgt tctgtttcat tctgggggat atagtagccc cctagcttgt tatctcgccc
     3061 accgcatacg ggtataccat aatcaacaat aagctggcga atatgttccc taatggttcg
     3121 gatgtccaag cctgtcagcc gttcaatatc tgtccccgtg ataggcaatt ccattccaag
     3181 cggtagtaac ttgaaaactt tgtataggtg tggtggtagt cgattttctg tcatacctgc
     3241 acctccagac ttgcaatttt gtattttatc caaagcaatt tactatcgtg ttccatatca
     3301 agatagcact gcattttttc tggtgttgtg tgtttgagaa tagtctctgc tatatgttca
     3361 agttctctaa atgtcagcat ggtttcccct cctagttata gcgtttgcct gcaagctgta
     3421 tataagcccc gtagcgctcg tttttaaggg gtctggtata tttaccctcg gtcttgattt
     3481 taggctctat atcaagccga aaagtgccca aaccaacgcc aaaccataga tagaggttca
     3541 gcggtgtaaa gattgctatc aaagttaaag ctgtttctat tgtcatttct tgcatttttt
     3601 tatctccttc ttcatatgct caagatcttg taagattagt ttcaagtacc acataggatt
     3661 acctacttca aacttgtctt catatccagc tttcaagagt gccacccttg cgctctcgac
     3721 tcggtgagtt ataccgttca aactgtgttc aatcggtgtt aaatcgccat acgggacacg
     3781 tataacatcc tcgtgaaagt ggttcagcat gatgtcggtt agatagtgca atgtccagcc
     3841 tgtttcatgg tctagttgat atactttgct gatgactgcc ttcagctctt ccgatattgc
     3901 attcttcacg ggtgcgtatt cttggttaga catttcaaaa tggattatcg tttctctgtt
     3961 attgtctttc atttagttct tgcctctgtt tcttttattg tttctgtgta atggcccgtg
     4021 tgggctgtct ccgtgagttc aaaagacttt gcttattggt tctttactat acgatttcta
     4081 tacctagcct agactattcc cagcggataa ccgccccaga cttaccaggt tcccctgtgg
     4141 tcgtgtaagc ctgtgccaaa ataatagcct tgctgtgtgt agtttgctta gggtggttta
     4201 gctttcgcta atggcgatac cagcacctaa tacttttctg ctccagtttt aaggggtagc
     4261 gccctctgta tggttataat gtcctagata tggtataatc tagctataaa aatctttact
     4321 aaaacccttt taataacagc ttgcctgctt gttaattttg ttttagttag tggttaaagg
     4381 ctttgctgat tggtctcggt aagccttttt ttgttgcttt cacgcgcctt gtggtgcgtt
     4441 ttttctgaat accatattct taatctcttg gtatgtcatg cctaagttga tcatagcaat
     4501 agccatgtct tctagtgcct ggtatctgat aagttcttga cttgttagac tgtcaatgcc
     4561 attaaatccg ccacggtgtg ccactagctg gcgtttgttc attccagtag ttccctttag
     4621 taaaaggttt gtaacagtgc tgtgcgcgtg ttttggagct tcctgccatg tttcaatagc
     4681 ttcatgtagt gccttacgct taggcttctc taatgccctc tgatagcgaa actgtgccac
     4741 ctcgtcacgc atttcaaaga atgctcggac taggtttgtt ttgaatttaa caacctgcgg
     4801 tgtgttgtcc agataagtga tcaacaaagt cgcctgttgt tcgtttaagt gatacacctt
     4861 tttgggtctg ccttttccgt ctaatttatg gatttcaaat ccataaaatc caaacgcctc
     4921 aaatcgttct ttgtgatttc tcaataggcg tgtaacagtg tggtgctgta ctccagcaca
     4981 ttctgcgatt atctcgcttg tggtatacgg ctctttccgt ccgtccatgt aaactaattc
     5041 cattgactaa cctccaatga ctgccactag cttagtcact cgctcaatcc ctgcggtaat
     5101 gtctgctagg ttatccattt cctgcacctc agcaagttta ttcttgatat aagttaggtc
     5161 actgatagct ggtaggggct gggttgtcgg ttggtcttga attagttcac cttgcatgta
     5221 gtagccgttt acacgaattt ctttcaagat gtttttgact acttttttaa actctttggc
     5281 ttttggtttg cgtgattgaa aaagtacttc ataaagtcca aactcagtta acatgtttac
     5341 ttctcgtttt tgacctgccc tagatattac ataggtcagc ttctcatctt catcaatcct
     5401 ttttaataag tcggttgtat tttgtacttc aatcatttca gccacttcaa cagcaataaa
     5461 aagtgggtta tctttgtttc cataaatttc aaatgactta tttccaaaat gttgagtagc
     5521 gataacctcg gtagtcggtt tctttttctt catttttgac cagtcgtaaa aatcttcgaa
     5581 tggttcaatt gtggtttgtg ttgtcatact ctgttttacc tctctgtata gattattgat
     5641 tttctttctg ctgtccttgt tgggttggtg tttgccctgt gcccacttgc caactgttcg
     5701 ggggtctgtt ccgatacgct gaccaatagc aaccaagttc agcccgtgaa tggttcgcat
     5761 ttcttcaatg actttactat agttccttct cattgcttgc ctgctccttt cttattcctc
     5821 tggcgttagt agttcatcaa tcgtcacgcc taaatagttt gcgacttttt gaagtactgt
     5881 aatatctgga tttttggtac gctcataata caattttgtc aacgtactct ttgaaattcc
     5941 tgtagcctcg tgaacgtgtg aaactttctc acggcgtttg gctaacaata cccgcatatt
     6001 gttcttcatg atcttttctc ctttctgttt tgataaccaa cctgaataga ttgaaaggtg
     6061 ttgcggttgg tttgcaccgt tattcggtgc tatgtttgta taatagcact ttattttttc
     6121 gcagtcaaca ctttttttga aaaatattgt acattttttc ggtgtcatgt tataatttgc
     6181 gtgaaaggtg ttgaaattat gaatagatta aaaattttac gcaaagaaaa aggcttgtcc
     6241 caacaagctc tagcgaaaga aattggtgtt tcatacagaa cactccaaaa ttgggaaaac
     6301 ggcgtcaatt ctatcaaacc agacaaagcc caggcactcg ctgaccattt tggggtaagt
     6361 gtcgggtatt tgttggggtt ctcctcggaa agttcaaaaa atgtactgac cgaattcaag
     6421 gaaggggagt actctcagat tgaagaggat agggcaaaaa taggaaatga taagttaata
     6481 ttgtactcaa ctataacaga tactcgacaa ttccttgata ggatgcaaag aaatattgat
     6541 atggatgcaa tcattaggaa actaccttta cccgaattgt taaagttaaa aaatccaaaa
     6601 gagagggaga agtttgcaag gaaacaaatg gaacctttca ttcgagaaat gaaaaacctg
     6661 gccattgctt tatcttattt gactgatgaa gaaagtgaat tgctagctag attttacgtc
     6721 ctaaacaaac aagataaaga cagacttttg caaatagcac gtaccttcac ggaagactaa
     6781 ccgctcacgc tctcaggggc tttctggtga ctgtgtggag tggaaaaaag tccgcaaaag
     6841 tccgcattaa aaaaagtcag tttttgtcag tagatgaaaa aagtcggatt ttgtcggttg
     6901 ctgaaaaaac tcaacaaaac tcaatagcta aaaaagtcaa gttttttcaa gttgtatatt
     6961 ttagtaaact gtctgcggtc gtgaaacggc acaatgtatt atatatcggc tataccttag
     7021 caaaccttag cattcggcgc gtgatactat catcatgata cgaacacaaa aagcattcta
     7081 aaaacatatt attctggtac tttgctaggt ttttacgctt caagggtggt ctatgaccgt
     7141 aaaaagaaag ataaaaccat ttgacaactg aataaggggg tgctatactt ggggtatagt
     7201 gatgaacgat tcacttccaa gtgattcaaa aaagccccca acgttgcacc gttgagggtt
     7261 tttgtttttg ccccgaaagg ctttacttgt tgcggttgtt tagccaatat tcaaacaagg
     7321 atgtcagaat actaaccagc aacggcaaaa gaaaaagtgt gatgaacaat tctttttcca
     7381 agcgactcac ctcccttcaa taaattgcgg gggcttgtct atcatatcag aataccgctc
     7441 gccctgtcaa acgctccaaa aatcgcctgt atcgtatttt ctcctcggct ggtataattt
     7501 tcctacccac caaaaacaaa cgaaaatagg gctattctcg taagcctacg catgataaaa
     7561 cctttatagc ttgcctgctg atggaaagga tatatcatga aaataactga agtaaaaaag
     7621 aaaaacggtg ctactgtgta ccgcgctagt gtgtatctgg gagttgacca ggtaacgggt
     7681 aagaaagtaa agaccaaagt aacgggtcgg accaagaagg aggttaaaaa caaaactcag
     7741 caagctattg caacttttaa aacagacggg gcaacacgct accaaaatgc caccataacc
     7801 agttacaaag agttggcaga attgtggtgg aatagctata agcacacagt aaaaccaaat
     7861 acccgtggga acatcagggg cttactgaaa aatcatgtta taccactttt cggagcctat
     7921 aagctcgata aactgacgac cccactcatc caaagcatcg tcatcaaatt agcagacaaa
     7981 gcaaatacag gggaagctgg tgcctatctg cactatgaca aaatccacgc gctgaacaaa
     8041 cgtatattgc aatacggtgt tgttatgcaa gttctgccat acaatccagc tcgggaagtc
     8101 atattgccca ggaacgcaaa aaaagccact cggaagaagg taaaacattt caacgacgag
     8161 cagcttaaac agtttcttga ctacttggac ggcttagact taaccaatta cagaaatctc
     8221 tatgaagcca ccctatacaa gttcttgctg gccactggtt gccgtatcaa tgaagcccta
     8281 gcgcttagct ggtcagatat tgacttgaac aacgccacca tttacatcac aaaaacactc
     8341 aaccgctacg gctcaataaa ctccccaaaa tcaaacgcta gcatacgcga tatagacatc
     8401 gatagccaga cagtagccat gctgaaagaa tacagatggc gacagataca agaggcttgg
     8461 accctcggac gttctgaaac ggtagtattt tccgacttta tccatgacta tcctgaggat
     8521 aagaccttag gaaataggct gacaacacgc ctcaagaata ttgggctgcc taatatcggt
     8581 tttcatggct tccgccacac ccacgctagt ctactgctaa actctggaat accctacaag
     8641 gaactccagc atcgtcttgg tcattctaga atatcaatga ccatggacat ctatagccac
     8701 ctttcaaaag agaacgcaaa gcatgctgtt acattttatg aaaaagctct agaaaacctt
     8761 tgagggtgaa caaaaagggg aacaaattcg gtttacagta gtttacagca ctgtaaaaat
     8821 ccttgatatg actgggtttc cagcgcgtta aacacaaatc ataccatttt gtgatataat
     8881 attctgtaac gaggtgactt atggaattta aattatttga tgactacatc acattacaag
     8941 cattgttaaa aacgactggt attctccatt caggaggtgc cattaaaggt ttcttagaag
     9001 aaaataccat tcttttcaat ggagaggatg aaaaaagacg cggtaaaaaa atccgtgtcg
     9061 gagatgttat cacactccca gaccacaaca ccagcatcac aatcgtcgct cctagtgcag
     9121 aggaaatcaa acagcaccaa gaggatcaag ctgaaaaaga acgtgtggct gcaatcgtta
     9181 aaaagttaaa tcaagaaaat aaaaagaata agaaacaggt aaaaacgcct aaaaaagcaa
     9241 ctaaaaatac tgaaagaaaa cctgttcgtt tccctggtat gtagacatgt ggctagaacg
     9301 attagagtta caacattttc gcaattacaa ccaattagat attgagttcc acaaagggct
     9361 caatgtcttt ttaggcgaaa acgcccaagg aaaaaccaat attctagaat ctatctatgt
     9421 tttagcctta acacgaagtc atcgaacacg gacagataag gacctactac agtttcaaga
     9481 aaaggaatta tctatttctg gtttactcca tcgcacaagc ggtaaagttc cccttgacat
     9541 tcacttgaca gacaaggggc gagtgaccaa ggtcaaccat ctcaaacaag ccaagctttc
     9601 caactatatc ggccacatga atgttgttct cttcgcccct gaagatttac agctgataaa
     9661 aggtgcgcct gctttgagaa gaaagttcat cgatgtcgag ctcgggcaaa tcaagccact
     9721 ctatctctct gacctatcga attataacca cgttttaaaa caaagaaata cttacctcaa
     9781 atctacagat aagattgatg agaacttcct atccgtgtta gatcagcaac ttgctgaata
     9841 tggtagtcgt gttatccaac atcgtattga ctttctaaag aaattagagg aattcggaaa
     9901 tagaaaagtt caagaaatat ctggcaatcg ggaagaattg acaatcgaat accaaacctc
     9961 aatcaaattg acagatgatg tcaatttaat tgacaaattc ttgacggaat tggaaaggtg
    10021 tcgcaaacgt gatttattta agaaaaatac aggagttgga ccacatcgag atgatgtagc
    10081 tttctttata aatggaatga atgcccatta tggtagccaa ggtcagcacc gcagtctcgt
    10141 tctgtcactc aaactggctg aaattgagct gatgaaggag gtcacacgag aatacccaat
    10201 tctactctta gatgatgtta tgagtgagct agataataat cgtcaaatca aactactaga
    10261 aactattaca gatacgattc aaacatttat tacaacgaca tctttagacc atttacataa
    10321 gctacctgat agtttaaaaa tcttccatat tgagtctggt aaggttactg agtcagaata
    10381 agattgttta cactgttgtc aataaagttt acaaaaaagt aaaaccctgc caagagggtc
    10441 ttgcagcacg tagacaaact cctaaaaata taaaaaattg aagaagtttg cgaaagatag
    10501 caagttatta aactcttaga aacattgata tttcggcgtt tctgagagtt ttttattttc
    10561 aacataaaga aaaaagattg aagaaaattg tcca
//
LOCUS       DAZQCJ010000063        10502 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000063 DAZQCJ010000000
VERSION     DAZQCJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10502
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.064"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(200..1690)
                     /gene="lysS"
                     /locus_tag="ACHU66_001456"
     CDS             complement(200..1690)
                     /gene="lysS"
                     /locus_tag="ACHU66_001456"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR3389733.1"
                     /translation="MSTEHFEELNDQQIVRREKMTALAEQGIDPFGKRFERSANSAEL
                     KAQYEDKSKEDLEELGQTAIIAGRIMTKRGKGKAGFAHIQDREGQIQIYVRKDDVGEE
                     NYEIFKKADLGDFIGVEGDVFKTNVGELSIHARKLTHLSKALRPLPEKFHGLTDIETR
                     YRKRYLDLITNRESFDRFVTRSKIISEIRRYLDGLGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADYLDIMDLTEGIIQHTAKAVVGDGPVTYQGTEIAIHEPFKRIHMVDAIKEQTGVD
                     FWQEMSFEEAKALAAEHKVPVEKHHTEVGQIINSFFEEYVEATLIQPTFVYGHPVAVS
                     PLAKKNDEDPRFTDRFELFIMTKEYGNAFTELNDPIDQLERFEAQAKAKELGDDEATG
                     VDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(1793..2419)
                     /locus_tag="ACHU66_001457"
     CDS             complement(1793..2419)
                     /locus_tag="ACHU66_001457"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFR3389734.1"
                     /translation="MKIYFVRHGKTEWNLEGRFQGYSGDSALLPESYQDLEKLGKYLA
                     EIPFDAIYSSDLQRAHSTAVEIAKANHHCQTVLTTQQLREWNFGTLEGSKMAIFRAIY
                     PKQAWALKHNLALFNNDLFEAESVRQVTQRMVDFVQSLKGQDMETVLIVSHGAFLTAS
                     IHRLLGFPPAQLRHRGGLDNASVTILETSDFENFTELAWNDTSYKAKQ"
     gene            complement(2422..2904)
                     /locus_tag="ACHU66_001458"
     CDS             complement(2422..2904)
                     /locus_tag="ACHU66_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFR3389735.1"
                     /translation="MAKKVKIKKTLVDQILDKAKINHDSLVLNAFEGQLPPGIEEHEI
                     YKTLALTGDKTGPIIGIVPITEHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNFPIYIDQSALELGHLIVSAGEIGRSIRIDSQVLADFVKADFADLIEGRD
                     "
     gene            complement(2904..3746)
                     /locus_tag="ACHU66_001459"
     CDS             complement(2904..3746)
                     /locus_tag="ACHU66_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFR3389736.1"
                     /translation="MASWISRIIKGMIIALGFILPGVSGGVLAAILGIYERLIGFLAN
                     IRKDFKENFLYFVPVGIGGILGIALFSFPVEFLLQHFQVPVLWAFAGAIVGTIPSLLK
                     ESTQKSKRDSIDLAWLIGTFIISGLLLYNLSDLVGTLPATFASFVLAGALIALGVLVP
                     GLSPSNLLLILGIYTPMLNGFKSLDLFGTFLPIAIGGVLAMVAFSKAMDHALKVYHSR
                     VYHFIIGIVSSSTLLILIPQASNKESISYAGANFVTWIAAIVLFILGAWLGLWMSKLE
                     EKYK"
     gene            complement(3849..4397)
                     /gene="thiT"
                     /locus_tag="ACHU66_001460"
     CDS             complement(3849..4397)
                     /gene="thiT"
                     /locus_tag="ACHU66_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFR3389737.1"
                     /translation="MSTSKLPILAEVAIFSAIALVFDKIPLFTMPQGGSVSLVMLPIL
                     LLALRHGLGVGVLTGGIVGTIQLFYGGYFLNVFQVFLDYALSYAGIGLAGLVAPTLSK
                     QKDLKNATLIITLASFLGGSIRLLATFLSGIIFYADYAPDGMPVWFYSFTYNISYILP
                     STAIASILLILLYRARPGFYNL"
     regulatory      complement(4456..4551)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(4575..5429)
                     /locus_tag="ACHU66_001461"
     CDS             complement(4575..5429)
                     /locus_tag="ACHU66_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFR3389738.1"
                     /translation="MRKKLNPMIVVGFFLSFFALIFITGVTGNTVNKTEVANEPAVTQ
                     SNTSTSSKTTNSSSSYYIANALEMKPIVDVSAWQRPSEIDYDTLSKNISGAIVRIQSG
                     SHTKNENTATDKNGLDKSFDTHIKEFQARNIPVAVYAYVTGNSIENMREEARSFYKAA
                     NKYKPTFYWLDVEEYTMDDMNAGVEAFRKELETLGAKNIGIYVGTYFMEDHSINVDKF
                     DAIWIPTYGDDSGYYNAAPNTDLNYDLHQYTSRGYVNGFEHHLDLNIITTLKDPNEVY
                     QKLFTTPE"
     gene            5542..6045
                     /locus_tag="ACHU66_001462"
     CDS             5542..6045
                     /locus_tag="ACHU66_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389739.1"
                     /translation="MHDKSEKVRRVSQLVVLSSLTIVLRVVFGPFPNIKPLTAIFLIV
                     LSYFSLLESWILMILVMVGSGLLFGFGTVVLWQVLSFAFIQFIWWLAIGPFIKKENIP
                     LWLQSLLAGILVFLYGLTISFLSATQFRTSPIVFWLNGLVFDSLHAASTVLFYPIIDY
                     IFRRFYK"
     gene            6042..6407
                     /locus_tag="ACHU66_001463"
     CDS             6042..6407
                     /locus_tag="ACHU66_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFR3389740.1"
                     /translation="MKKISIMLMVVFSLFLAACTNTTEKQSESAIEVTLQITTKDKAN
                     HQEVTAEEGDSVMDVLKEHHDIVEDNGLITEIDGVSQDASTNTYWMYKVNGQMADKGA
                     KDLTVANGDEIEFYLETFE"
     gene            6544..7758
                     /locus_tag="ACHU66_001464"
     CDS             6544..7758
                     /locus_tag="ACHU66_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFR3389741.1"
                     /translation="MKIDKRQLLNYSILIPYLILSVIGLVMVYSTTSATQIINGGNPF
                     RTVINQAGFWIVSLVAIYTIYRMKLSFLRKKAVIYSVILVEVFLLAISRLFPPINGAH
                     GWIPLPIAGTLQPAEYLKLIIIWYLAHEFAKQQADIRTYDYVSLIKGSWIPKEFTDWR
                     VVSLLLLGLVATLPDLGNATIIVLIMVVMISVSGIAYRWFSTAVLGIVAASTVILGTI
                     RILGVETVEKIPLFGYIARRFAAYFDPFGNATNSGLQLTHSYYAMSNGGWLGRGLGNS
                     IEKKGHLPEAHTDFAFSIVIEELGFVGASLILALLFFLIIRIIIVGVRARSPFNAMMA
                     LGMAGMLLIQTFVNIGGISGIIPATGVTFPFLSQGGSSLLIISIGIGFVLNIDASEKR
                     RLIEEEIERTLI"
     gene            7779..10475
                     /gene="ppc"
                     /locus_tag="ACHU66_001465"
     CDS             7779..10475
                     /gene="ppc"
                     /locus_tag="ACHU66_001465"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR3389742.1"
                     /translation="MAIQKLENYNNKEAIREEVTILTSILEKVAAQMMPAETFAKIVE
                     LSQLSRQDDYQALIAIISQLNNDELAVISRYFAVLPLLINISEDVDLAYEINHQNNID
                     QDYLGKISTTIDMVSKQENAAEILEKLNVVPVLTAHPTQVQRQSMLDLTKHIHELLRR
                     YRDVKLGLLNKNKWEDELRCYIEIIMQTDMIREKKLKVTNEITNVMEYYNSSFIKAVT
                     KLQREYKRLAAEKGIVLNNPRPITMGMWIGGDRDGNPFVTAETLKLSALTQCEVIMNY
                     YDDQLYKLYRNFSLSTSIVNVSTAVKMLADLSEDSSVYRENEPYRRAFHYIQMKLANT
                     RDYLVHNKPSDVRYSNVAEFKADLLAIKQSLVENKSMALLKGDFTELLEAVEAFGFYL
                     ASIDMRQDSSIHEASVAELLASARIVEDYSSLSEEAKCHVLLKQLETDPRILSATHMP
                     KSEQLEKELAIFAAARELKDKLGEEIIKQHIISHSESVSDLLELAVLLKEVGLVDVDK
                     ARVQIVPLFETIEDLDNAPDTMRQYLQLDLAKRWIAGNKYYQEIMLGYSDSNKDGGYL
                     SSGWTLYKAQNELTQIGQDYGVNITFFHGRGGTVGRGGGPSYDAITSQPFGSIKDRLR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSATLDRMVTQMITDPNEIDGYRETMDEIVSDS
                     YRIYRDLVFNNPHFYDYFFAASPIREVSSLNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QNRVMLPGWYGVGSSFKRFIDKHPDNLSKLQKMYESWPFFRSLLSNVDMVLSKSNMNI
                     AFEYAKMCESEEVRNIFHVILDEWQLTKEIILSIEQNDELLAELPFLKASLDYRMPYF
                     NVLNYIQIELIHRLRRGELSKEQENLIHITINGVATGLRNSG"
BASE COUNT         3038 a         1975 c         2299 g         3190 t
ORIGIN      
        1 atgtggttgg gatttttttg catgcaaaaa cactcttgga atttccaaga gtgttttaag
       61 taacggtgtt aaacagtttg taatttttat ttcatcgtcg ggagtgaaaa ggtctagtag
      121 accttttcag ccaatcgcta agaaactgga gagcgattgg tacattctag tgaaaacaat
      181 cctagcaagc tactcttact tacttcatag tcggaaatag gagtacatcc cgaatagttg
      241 ttgtgtctgt taagagcatg cagaggcggt cgataccgat tccaagtcca cctgttggtg
      301 gcataccgta ttcgagggct tcgatgtagt cgtagtcaac gcctgtcgct tcgtcgtcac
      361 cgagttcttt agctttggct tgggcttcga agcgttccaa ttggtcgatt gggtcgttca
      421 attctgtaaa ggcatttccg tattccttgg tcatgatgaa gagctcgaag cgatctgtga
      481 agcgtgggtc ttcatcgttt ttcttagcca gtggggatac ggctactggg tgaccataga
      541 cgaaggttgg ttggataaga gttgcttcaa cgtattcttc aaagaagctg ttgatgattt
      601 gaccaacttc tgtatggtgt ttttccacag ggaccttgtg ctcagctgca agggcttttg
      661 cttcctcgaa gctcatctct tgccagaagt ccacgccagt ttgttcctta atggcatcaa
      721 ccatgtggat acgcttgaat ggctcgtgga tagcaatttc agtgccttga taagtaacag
      781 gaccatcgcc gacaacagcc ttggcagtgt gctgtataat accttcggtc aagtccatga
      841 tgtccaagta atctgcataa gcttggtaga cctcgattga cgtgaactca gggttgtgag
      901 tcgcatccat accctcgtta cggaagatac gtccgatttc ataaacgcgt tccataccac
      961 cgacaatcaa gcgtttgagg tggagttctg tcgcgatacg aaggaccatg tcaatgtttt
     1021 gggcattgtg gtgggtgatg aatggacgag ctgcagcacc accagcttca ttgtgaagga
     1081 caggtgtttc cacttccaag aaaccaagtc cgtcaaggta acggcggatt tctgagataa
     1141 tttttgagcg ggtcacaaag cgatcaaagc tctcgcggtt agtaatcaag tccaaataac
     1201 gtttgcggta gcgggtctcg atgtctgtca aaccgtggaa tttttctggc aatgggcgga
     1261 gcgctttaga caagtgagtc aacttgcgag catggataga caactcacca acgtttgtct
     1321 tgaagacatc tccctcgaca ccgataaagt caccaaggtc tgcttttttg aagatttcat
     1381 agttttcttc gccaacatca tccttacgaa cgtagatttg aatctggcct tcgcggtctt
     1441 gaatgtgagc aaaacctgcc ttacccttac cacgcttggt cataatacga cctgcaatga
     1501 ttgctgtttg ccctaattct tccaagtctt cttttgattt gtcttcgtac tgggccttca
     1561 attcagctga gttggcagaa cgctcaaaac gttttccaaa tgggtcaatg ccttgttcag
     1621 caagagccgt cattttctca cgacggacaa tctgttggtc atttaattct tcaaaatgtt
     1681 cagttgacat aaaaatttta ttcctccaaa gtctgttact tctattttat catagaagag
     1741 agaaaaaatc agtataaatt gctaatagga taggaaaaaa cagcagaaaa ttctactgtt
     1801 ttgctttata gctggtatca ttccaagcta attctgtaaa gttttcgaaa tcgcttgttt
     1861 ctaaaatagt aaccgatgca ttatccaaac cacctcggtg acgcagttgg gctggtggaa
     1921 aaccaagcag gcgatggata gaagcggtta gaaaggcacc atggctgacg atgaggactg
     1981 tttccatgtc ttgccctttc agcgattgga caaagtccac cattcgttga gttacctggc
     2041 gaacgctctc agcttcaaat aagtcattgt taaaaagtgc tagattatgt ttgagtgccc
     2101 aggcttgttt ggggtagatg gcacgaaaaa ttgccatctt gcttccttct agcgttccaa
     2161 aattccattc acgtagttgt tgcgtggtta gaacggtttg gcaatggtga ttggctttgg
     2221 caatttctac ggcagtgcta tgtgcgcgct gcaaatcact agaataaata gcatcaaagg
     2281 ggatttcagc aaggtatttg cctagttttt ctaaatcctg ataagattca ggcaagagag
     2341 ctgaatctcc agaatagcct tgaaagcgtc cctccaaatt ccattcggtc ttaccgtgac
     2401 ggacaaagta gattttcaag tctagtccct cccctcgatc aaatcggcaa agtcagcttt
     2461 tacaaaatca gctaatacct gactgtcaat tcgaatcgac cgaccaattt ctccagcaga
     2521 gacaatcaag tgaccgagtt caagggcaga ttgatcaatg tagattggga aattatgctt
     2581 ctgacgaatg cctacgggat tgttggcacc atggatgtag ccagttgtct tttccaagtc
     2641 cttttgcgga atcatactca cttttttatt gccagagatc ttggctaatt ttttctcaga
     2701 caggtgttct gtgattggga caatgccgat aattggtcca gttttatctc cagttaatgc
     2761 cagtgttttg taaatttcgt gttcttcaat tcctggtgga agttgacctt cgaaggcatt
     2821 taaaacaagg ctatcatgat tgatcttagc cttgtccaat atttggtcca ccaaggtttt
     2881 ctttatttta accttttttg ccattattta tatttttcct caagtttact catccaaagc
     2941 cctaaccatg ctcctagtat aaataggaca atagcagcaa tccatgtaac aaagttcgct
     3001 ccagcatagg aaattgactc tttattactt gcttgtggaa tcaaaattag gagagtagaa
     3061 gatgaaacga taccaataat aaagtgataa acacgtgaat ggtatacttt cagagcgtgg
     3121 tccattgctt ttgaaaatgc aaccattgca agaacaccac cgattgcgat tggtaggaag
     3181 gtaccgaata gatcaagtga tttgaaacca tttagcattg gcgtgtagat acccaaaatc
     3241 aatagtaagt ttgaagggct gagtccagga acaagtaccc ctaaagcgat caaggcacct
     3301 gccaatacaa aactagcaaa agtagctgga agtgtcccga caagatcgct caaattgtaa
     3361 agtaggaggc cagaaataat gaatgtccca atcaaccaag ccaaatcaat gctatctcgt
     3421 ttgctctttt gagttgattc tttaagtagg ctaggaatag ttcccacgat tgctcccgca
     3481 aatgcccaca aaactgggac ttggaaatgt tgcaataaaa attctacagg gaaagagaaa
     3541 agtgcgattc ccaaaattcc accaattcct acaggaacaa aataaagaaa gttttccttg
     3601 aaatcttttc gaatatttgc taaaaatcca attagtcgct catagattcc gaggatagca
     3661 gcgagtaccc ccccagagac accaggtaag ataaagccga gtgcgataat cataccttta
     3721 ataattcttg aaatccaaga agccataatt tcctccaaaa tgtattataa cgttcctatt
     3781 atagcataaa gcaagtaaaa ataattaaaa agactgcctg ttagataaaa tccagcaggc
     3841 agttttggtt aaagattgta aaaacctggt cttgctctat agagtaaaat gagcagaata
     3901 gaggcaattg cagtggaagg aagaatatag ctgatattgt aggtaaagga atagaaccag
     3961 acaggcatac catctggagc gtagtctgcg tagaaaataa ttcctgataa gaaggtggct
     4021 aacaatcgga tgcttcctcc tagaaaacta gccagagtaa tgatgagagt agcattttta
     4081 agatcttttt gtttggatag ggtgggtgcc actaaacctg ctaaaccaat tccggcgtaa
     4141 gaaagtgcgt agtcaagaaa aacttgaaaa acgtttagga agtagccacc gtagaaaagt
     4201 tgaatggttc cgacaatacc accagtaaga acccccaccc ctagtccgtg gcgcagagca
     4261 agcagtaaga ttggaagcat gactaaagac acagatccac cttggggcat tgtaaagagc
     4321 gggattttgt caaagacgag ggcaattgca gaaaagatag cgacttcggc taggatgggt
     4381 aattttgatg tggacataaa aagactcctt tgtagatggt caacaaagga gtgtttatag
     4441 cagagtatag tatcattcta taaagcacac aattcctacg ctagttttaa ctagatcagg
     4501 ttcgaggatt tcgcagttgc gatctcagcc gaagcactcc tttgtgtata tgtaattagt
     4561 gagattgtag cattttattc gggtgttgta aatagtttct gataaacttc attaggatcc
     4621 ttcagggtag taataatgtt gagatctagg tggtgctcaa agccgtttac ataaccacgt
     4681 gaggtatatt ggtgaagatc gtagttcaag tcagtatttg gagcggcatt gtagtagcca
     4741 gaatcatcac cgtatgtagg aatccaaatg gcgtcaaatt tatccacatt gatgctgtgg
     4801 tcttccatga aataggttcc gacgtaaatt ccaatgtttt tagcgccgag agtttctaac
     4861 tcttttcgaa aagcctccac tccagcattc atatcatcca tggtatattc ttcaacgtct
     4921 aaccaataga aggttggctt gtatttattt gcggccttat aaaagcttcg agcttcctct
     4981 cgcatgttct caatactatt tcccgtgaca taggcataga cagcaacagg tatatttctt
     5041 gcttggaatt ctttaatatg ggtatcaaag gatttatcta acccattttt atcagtcgca
     5101 gtattttcgt ttttagtgtg tgatccactt tgaatacgaa cgattgcgcc agaaatgttt
     5161 ttgctaaggg tatcgtagtc aatttcagac ggtctctgcc aagcagaaac gtcgacgata
     5221 ggtttcattt cgagcgcatt tgcaatgtaa taacttgagc tagaatttgt tgttttagaa
     5281 cttgtactgg tgtttgattg tgtgactgcg ggttcgttag ctacctccgt cttatttaca
     5341 gtatttccag tcaccccagt gatgaaaata agagcaaaga aggataggaa aaaaccaaca
     5401 acaatcatcg gatttagttt ctttctcatc tcctcaattc taccgtaaaa tcatgaaaaa
     5461 atcaaaaaaa tacgattcaa gaaatagtaa aagattatct gaacttgaca taataaaggg
     5521 aataaggtag gctaaattgg tatgcatgac aaaagtgaaa aagtaagacg tgttagtcag
     5581 ctagttgttc tatcctcttt gacgattgtt ttaagggtgg tttttgggcc atttcccaat
     5641 ataaaacctc tcacggctat tttcctcatt gttctgtcct atttcagttt gctggagagc
     5701 tggatattga tgatactagt gatggtaggg agtggcttgc tatttggatt tggcacagtt
     5761 gttttgtggc aagttcttag ttttgctttt atccaattta tatggtggtt ggcaatcggt
     5821 ccttttatca aaaaagagaa cattcccttg tggctacaga gtcttctggc ggggattctt
     5881 gtctttttgt atgggctaac gataagtttt ttatctgcta cacagtttag gacttcccct
     5941 atcgtcttct ggcttaatgg tctagtcttt gatagtttac acgctgcttc aacagttttg
     6001 ttttatccaa ttattgatta tatttttagg agattttata aatgaaaaaa attagtatta
     6061 tgttgatggt tgtcttctcg ctcttcttgg ctgcttgtac aaacactact gagaaacaga
     6121 gtgaatcggc tatcgaagtc actttgcaga ttacaaccaa ggacaaagcg aatcatcagg
     6181 aagtcactgc ggaagaaggt gatagtgtca tggatgtgct taaagaacat catgacattg
     6241 tagaagacaa tggtttgata acggaaattg atggtgttag ccaagatgcg agtaccaata
     6301 catactggat gtataaagtt aatggccaaa tggctgataa aggggcgaaa gaccttacag
     6361 ttgcgaatgg ggatgaaatt gaattttatc ttgaaacatt tgaatagata tgaaaggctt
     6421 cggccttttt cttgtcgaca aaagtcagtt ccgaatgttt gcagagcaac ttagaaagtg
     6481 ataagtattt ttatacaaag tatgctataa taagagctat actcgaagaa ggttggataa
     6541 gcaatgaaga ttgacaaacg ccagttgttg aattattcca tactgattcc ctatctcatc
     6601 ttatcggtga ttgggttggt catggtctat tcgacaacca gtgctacgca aattattaat
     6661 ggtggaaatc catttagaac agtgattaac caggcaggat tctggattgt cagcttggta
     6721 gcaatctata cgatttatcg tatgaaatta agttttttac gcaagaaagc cgttatctat
     6781 tcggttattt tggtagaagt atttctgtta gctatttctc ggttgtttcc tccgattaac
     6841 ggagctcatg gctggattcc tctgccaatc gcaggaacct tgcagccggc agaatatttg
     6901 aaattaatta tcatttggta tctggcacat gagtttgcaa aacaacaggc ggatattcgg
     6961 acttatgact atgtatcgtt gattaaagga agttggattc cgaaagagtt tacagattgg
     7021 cgggtggtca gtctgctcct ccttggtcta gttgcgacac tgccagactt gggaaatgcg
     7081 acgattattg tgctgattat ggtcgtgatg attagtgtga gtgggattgc ctatcgctgg
     7141 ttttcaacag cggttttggg aattgtggcc gcctcaacag ttattctagg gacgattcgt
     7201 attttgggtg tagaaacggt ggaaaaaatt ccactctttg gttatattgc tcgtcgtttc
     7261 gcggcctatt ttgatccatt tggtaatgcc accaactcag gtcttcaatt gactcattct
     7321 tactatgcga tgagtaacgg tggatggctt ggacgtggct tgggaaattc gattgagaaa
     7381 aaggggcatc taccagaggc ccacaccgac tttgcgtttt cgattgtcat tgaagaactc
     7441 ggttttgtcg gagctagttt aattctagcc ttattgttct ttttgattat tcgaattatt
     7501 attgtaggcg tccgtgctcg tagtccattt aatgcgatga tggcacttgg aatggcaggt
     7561 atgcttctaa ttcaaacatt tgtcaatatc ggaggaattt caggaattat tcctgcaacg
     7621 ggggtaactt tccccttctt gtcacagggt ggatcgagtc ttttgattat ttccatcggg
     7681 atagggtttg ttctgaatat tgatgctagt gaaaaacgtc gattaattga agaagaaata
     7741 gagcgtacgc taatttagag ataggaaaga ggtaggagat ggctattcaa aaattagaaa
     7801 actataacaa caaggaagca atccgtgaag aagtaacgat tctaacatct attttggaaa
     7861 aagtagcagc ccaaatgatg cctgcagaga cctttgctaa aattgtagaa ttatctcaat
     7921 tgtctcgtca agatgattat caggcattga ttgctattat cagtcagttg aataatgatg
     7981 aactagcggt tatttctcga tactttgctg ttttgccact tttgattaat atatcagaag
     8041 atgttgactt ggcctatgaa atcaaccatc aaaacaatat tgatcaagat tatcttggta
     8101 agatttcaac aacaattgat atggtttcca aacaagaaaa tgcagctgaa atccttgaaa
     8161 agttgaatgt tgttccagtc ttgacagccc atcctactca ggttcaacgc cagtctatgc
     8221 ttgatttaac caagcatatt catgagttgc ttcgtcgtta tcgcgatgta aaattgggct
     8281 tgctcaataa aaacaaatgg gaagatgagc tccgttgtta cattgaaatt atcatgcaga
     8341 cggatatgat tcgtgagaag aagttgaagg taaccaacga aatcacaaac gttatggagt
     8401 attataattc ttcctttatt aaggctgtca ctaagttgca gcgggaatac aaacgtttgg
     8461 ccgctgagaa aggaatcgtc ttaaataatc ctcgtccaat tacaatgggt atgtggattg
     8521 ggggagaccg tgatggaaat ccatttgtta ctgcagagac cttgaagctg tccgccttga
     8581 ctcagtgtga agtcattatg aattattacg atgatcaact ctataaactc tatcggaatt
     8641 tttcactctc aacttccatt gtaaatgtat ctacagcggt taagatgctg gcggatttat
     8701 ctgaagatag ttctgtctac cgtgaaaatg agccttatcg tcgtgctttt cactatattc
     8761 agatgaaact tgcaaataca agagattatc tagttcacaa taagccgtca gatgttcgct
     8821 atagtaatgt agccgagttt aaagctgact tattagccat caaacaatct ttggttgaaa
     8881 ataaatctat ggcgctattg aaaggtgatt ttacagaatt gcttgaagcg gttgaagcgt
     8941 ttggtttcta cctagctagt atcgatatgc gccaagactc ttctattcat gaagcaagtg
     9001 ttgcggagct attggcctct gcccgtatcg tagaagacta ctccagttta tctgaggaag
     9061 ctaaatgcca tgttcttttg aaacagttgg aaactgatcc acgcattctt tctgcaacgc
     9121 acatgccaaa gtcagaacaa ttagaaaaag aattggccat ctttgctgct gcgcgtgaat
     9181 tgaaagataa gctaggtgag gaaatcatca agcaacatat tatttcacac tcggagagtg
     9241 tttctgattt gctggaatta gctgtcctat taaaagaagt tggtctagta gatgtcgata
     9301 aggcccgtgt tcagattgtt ccactttttg agacaatcga ggacttagat aatgcacctg
     9361 ataccatgcg tcaatatctg caattagatt tagcgaaacg ttggattgca ggcaataaat
     9421 actaccaaga aatcatgctt ggctactcag attccaacaa ggatggtggc tacctatcct
     9481 ccggctggac actctacaaa gcacagaatg aattgacgca aattggtcaa gactacggag
     9541 taaacatcac cttcttccac ggtcgtggtg gtactgttgg tcgtggtggg ggtccttcct
     9601 atgatgcgat tacatctcag ccatttggtt ccatcaagga ccggcttcgt ttgacagaac
     9661 aaggcgaagt gataggtaat aaatacggta acaaagacgc agcctactat aatttagaaa
     9721 tgctggtatc tgcaacgctt gaccgtatgg tcacacaaat gattaccgat ccaaatgaaa
     9781 tcgatggcta tcgcgaaacc atggatgaaa ttgtatcaga tagctatcgt atttatagag
     9841 atcttgtgtt caataatcca catttttatg attatttctt tgccgctagt ccaattcgtg
     9901 aagtatcaag tctgaacatt ggctcacgtc cagcggctcg aaaaaccatc acagaaatcg
     9961 gtggtttgcg tgcgattcca tgggtattct cttggtcaca aaaccgtgtc atgttgccag
    10021 gttggtatgg agttggttca agtttcaaac gctttattga taagcaccct gataatttgt
    10081 ctaagttaca aaaaatgtac gaatcttggc cattcttccg ttcacttcta tcaaacgtgg
    10141 atatggtatt gtctaaatcc aatatgaata tcgcatttga atatgctaaa atgtgtgaaa
    10201 gcgaagaggt acgaaatatc ttccatgtca ttttagatga atggcagttg accaaagaaa
    10261 ttattttgtc aatcgagcaa aatgatgagc ttttagcaga actaccgttt ctaaaagcta
    10321 gtcttgacta tcgcatgcct tatttcaatg tgctcaacta tattcagatt gaattaattc
    10381 atcgtcttcg tcgtggagaa ttgagcaaag aacaagagaa tctcattcat attaccatca
    10441 atggtgtggc gacaggtttg cgcaactcag gttaataggc ataaaacaac ttcttcggag
    10501 gt
//
LOCUS       DAZQCJ010000064        10435 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000064 DAZQCJ010000000
VERSION     DAZQCJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10435
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.065"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(215..1237)
                     /gene="ilvC"
                     /locus_tag="ACHU66_001466"
     CDS             complement(215..1237)
                     /gene="ilvC"
                     /locus_tag="ACHU66_001466"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR3389743.1"
                     /translation="MTVTMQYEKDVTVAALDGKRIAVIGYGSQGHAHAQNLRDTGHDV
                     IIGVRAGKSFDKAKEDGFETFEVAEAAKQADVIMILAPDEIQADLYNEEIAPNLEAGN
                     ALGFAHGFNVHFEFIKVPADVDVFMCAPKGPGHLVRRTFEEGFGVPALYAVYQDATGN
                     AKHIAMDWAKGVGSARVGLLETTFKEETEEDLFGEQAVLCGGLTALMQAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLVYEGGFKKMRQSISNTAEFGDYVSGPRVITDQVKENMK
                     AVLADIQSGKFANDFVNDYKAGRPRMEAYRKEAENLEIEKVGAELRKAMPFVGRNDDD
                     AFKIYN"
     gene            complement(1369..1845)
                     /gene="ilvN"
                     /locus_tag="ACHU66_001467"
     CDS             complement(1369..1845)
                     /gene="ilvN"
                     /locus_tag="ACHU66_001467"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR3389744.1"
                     /translation="MRRMLTAKLRNSSGVLNRFTGVLSRRQINIESISVGPTEVDGIS
                     RVTVIVDVVSHDEVEQIIKQLNRLIDVVRVRDLTDIPHLEREVILVKISAPPTKRAEI
                     LAIIQPFRANVVDVAPHSITIQMTGDGDKIDALLRVIQPYGIKNIARTGATGFSRD"
     gene            complement(1838..3541)
                     /locus_tag="ACHU66_001468"
     CDS             complement(1838..3541)
                     /locus_tag="ACHU66_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR3389745.1"
                     /translation="MQEIQLDQPRSGSYLILDTLHQLGVDLVFGYPGGAVLPLYDAIY
                     QYEGIQHILARHEQGAVHEAEGYAKSSGKVGVAIVTSGPGATNAITGIADAMGDSVPL
                     LVFTGQVATRGIGKDAFQEADVLGMTMPITKYNYQIRDTADIPRVITEALHIATTGRP
                     GPVVIDVPKDIQERVVDFYHDPTLHLPSYQPTIEPNGLQVKKILKQLSQAQKPVILAG
                     GGVNYADANKELVAFAERYRIPVVSTLLGLGAMPIEHELSLAMGGMHGSYAANMAMDQ
                     ADYIINIGARFDDRLTGNPTTYAPNATVAHIDIDPAEIGKVVKTAIPVVGDAKATLKA
                     LLALETVETGYADWTAQVLENKRRAPFWYDEDEKVIKPQVAIELIGQLTQGDAIVVTD
                     VGQHQMWAAQFYPYKHARQLVTSGGMGTMGFGIPAAIGAKLANPDKEVIIFVGDGGFQ
                     MTNQELAILNGYGVPIKVVLINNHSLGMVRQWQESFYEKRRSQSVFDDEPNFQLLAEA
                     YGISHYSFDNPATLSEDMKVILEDKPMLIEVHISNREHVQPMVPAGKSNAEMLGVKFN
                     A"
     gene            3766..4932
                     /locus_tag="ACHU66_001469"
     CDS             3766..4932
                     /locus_tag="ACHU66_001469"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR3389746.1"
                     /translation="MYYVVIPAYQPDEKLIQLLEIMKNRLNCQVIVVDDGSTGASKNI
                     LEIAKTYAIVLHHDVNKGKGQALRTAFSFIQDLRKPGVIVTADADGQHTVNDIDRVAR
                     AAMNLPSRLILGVRQFTKDIPLRSRFGNKLTRVLFRLQTGVNVSDTQTGLRGFHTDLI
                     PFMLDIEGDRYEYEMNMLTQASQRYKITEVPIQTIYIDDNASSHFRPIKDGLLIYKNL
                     FKFALASFGGFLVDYGIYALALLLLTSLPTALRLLVANTIARICSATCNFALNKKLVF
                     NNEESIARTGAGYFTLALVLFLCDTALLYLFHQILGLNLYIVKLAVDIFLFLASWFVQ
                     KNIIFKERKSFSHEIAKKNLCLRSHLRNAFDRRLYLLHVKNLRAFRGYHNGCCK"
     gene            4850..5738
                     /locus_tag="ACHU66_001470"
                     /pseudo
     CDS             4850..5738
                     /locus_tag="ACHU66_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445339.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
     gene            6291..8006
                     /gene="ilvD"
                     /locus_tag="ACHU66_001471"
     CDS             6291..8006
                     /gene="ilvD"
                     /locus_tag="ACHU66_001471"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR3389747.1"
                     /translation="MTDTNKLKDFRHRSSVYDSMVKSPNRAMLRATGMTDDSFEKPIV
                     GVISTWAENTPCNIHLHDFGKLAKEGVKDAGAWPVQYGTITVADGIAMGTPGMRFSLT
                     SRDIIADSIEAAMGGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAVFAYGGTIAPGNL
                     NGKDIDLVSVFEGIGKWNNGDLTAEEVRQIECNACPGPGGCGGMYTANTMATAIEVMG
                     MSIPGSSSHPAESPEKKADIEEAGRAVVRMLELGIKPSDIMTREAFEDAITVTMALGG
                     STNATLHLLAIAHAANVELTLEDFNDFQERVPHLADLKPSGKYVFQDLYNVGGVPAVM
                     KYLLKNGFLHGDRITCTGKTVAENLENFADLTPGQDVIMPLENPKRADGPLIILKGNL
                     APEGAVAKVSGVKVRNHTGPAKVFDSEEEAIEAVLTDEIVDGDVVVVRYVGPKGGPGM
                     PEMLSLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDLV
                     TVDQDTKEITMHVSDQEIEDRKKTTVIPPLYSRGVLGKYAHTVSSASKGAVTDFWRPE
                     RTGKK"
     gene            8057..8443
                     /locus_tag="ACHU66_001472"
     CDS             8057..8443
                     /locus_tag="ACHU66_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6176 family protein"
                     /protein_id="HFR3389748.1"
                     /translation="MKLNVELSRFRVKEGKTVQVDEWMAFLNEHMEDTLLTLEGEKMY
                     VETIFREVLDGREYLYWYSVQAEGRIEVEDSESYIDKKHLEYWEECIDPSYGMVGLDP
                     QVIMIHKTIYETMEELDRQYDETFKK"
     gene            complement(8470..8811)
                     /locus_tag="ACHU66_001473"
     CDS             complement(8470..8811)
                     /locus_tag="ACHU66_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFR3389749.1"
                     /translation="MREDIQINERALTVSEQLVEVLESIYDPEIELDIYNLGLIYEIH
                     LDEAGFCKVVMTFTDSGCSCADTMPGELVAALKTIDGINDAQVEIVWSPAWKMTRISR
                     LGRITLGISPK"
     gene            complement(8933..9352)
                     /locus_tag="ACHU66_001474"
     CDS             complement(8933..9352)
                     /locus_tag="ACHU66_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFR3389750.1"
                     /translation="MELKDIMHILEDMKVGVFATLDEYGNPHARHAHITAANEEGIFF
                     MTSPETHFYDQLMGDQRVAMTAISEEGYLIQVVRVEGTARPVENDYLKTVFADNPYYQ
                     HIYKDESSDTMQVFQIYAGHGFYHSLPQGHKYIFSIG"
     gene            complement(9903..>10435)
                     /locus_tag="ACHU66_001475"
     CDS             complement(9903..>10435)
                     /locus_tag="ACHU66_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389751.1"
                     /translation="YEDIDTVVVHEYLQQILLFDYDDPTETIRYRLKQYRIEYSDMFP
                     KNMNDAELLRFIIYGYFSQAMDVFLTSYTFNAFPFFSSLTTYLNYNVLLLNIVHYSKQ
                     ENLEHFLKMSKLTLPIWFQYNQKGVELSISEISERAKNINFSDRIREIYNALDDFNTQ
                     EQLITEIENCVHLLIK"
BASE COUNT         3072 a         2325 c         1984 g         3054 t
ORIGIN      
        1 attatgtcat tttataaaaa ccgaactttt ttattttttt aatgtattca tgaatgaaat
       61 gggcaaaaga gccggaaaaa accagctctt ttattcattt tcagagatag gggaaaaaag
      121 ggaatgacat cccctcatcc gccaacccaa accttaaatg tattgccatc actcaaagta
      181 aagctgacta tccccctatc taccatgatt gtgtttaatt atagattttg aatgcgtcgt
      241 catcattgcg tccgacaaat ggcattgctt tgcggagttc tgcacctact ttttcgattt
      301 caagattctc tgcttcttta cgataagctt ccatacgtgg acgacctgct ttatagtcat
      361 ttacaaagtc atttgcaaat ttaccagatt gaatatctgc aaggactgcc ttcatgtttt
      421 ctttgacttg gtctgtaatc acgcgtggac ctgatacata gtcaccaaat tcagcagtgt
      481 ttgaaataga ttggcgcatt ttcttaaagc caccttcgta gacaaggtca acgatgagtt
      541 tcatctcgtg gagaacttcg aaataagcca attctggtgc ataaccagct tctgtcaaga
      601 cttcaaaacc tgcctgcata agggctgtca aaccaccgca gagaacagct tgctcaccaa
      661 acaaatcttc ttctgtctct tctttgaaag ttgtttccaa aagaccaaca cgggctgaac
      721 caacaccttt tgcccagtcc atcgcaatat gttttgcatt gccagttgca tcttggtaaa
      781 cagcatagag ggctggtact ccaaaacctt cttcaaaggt acggcggacc aagtgacctg
      841 gaccttttgg tgcacacatg aagacatcca catctgctgg taccttgata aattcaaagt
      901 gaacgttgaa accatgggca aaaccaagag cattgcccgc ttccaagttt ggagcgattt
      961 cttcattgta aaggtctgct tggatttcgt ctggagccaa gatcatgata acatctgctt
     1021 gttttgctgc ttctgctact tcaaaagttt caaaaccgtc ttcttttgcc ttatcaaatg
     1081 acttacctgc acgcacaccg atgatgacat cgtgccctgt atcacgcaag ttttgagcat
     1141 gggcatgacc ttgtgaacca taaccgataa cggcgatacg tttaccgtca agtgctgcta
     1201 ctgttacatc tttttcatat tgcattgtta ctgtcataat tttttctctt ttctattttt
     1261 atttgtagtt ttggatgttc aaagaaaatg aaggctagac taggcgaaga tagaactaac
     1321 gttcatcaag cgaagtcaac gcaggatagg ttttattatc gaaaaggatt agtctctact
     1381 aaagccagtc gcacccgttc ttgcgatatt cttgatgcca taaggctgaa tcacacgcag
     1441 gagggcgtca atcttatctc catcacctgt catttgaatg gtaatggagt gcggtgccac
     1501 atcaacaaca tttgctctaa agggttggat aatcgctaaa atttctgctc gttttgttgg
     1561 cggagctgaa attttaacaa gaatcacctc acgctctaag tggggaatgt ccgtcaaatc
     1621 acggacacgg accacatcaa tcaagcggtt cagctgtttg atgatttgtt cgacctcatc
     1681 gtgactgacc acgtctacaa tcaccgtcac acgtgagatg ccatctactt ctgttgggcc
     1741 gacggagatg gactcaatat tgatttggcg acgagatagg acacctgtaa aacgattcag
     1801 aacaccagat gaatttcgca atttagctgt taacattcta cgcattgaac ttcaccccca
     1861 acatttctgc attgctctta cctgctggca ccatcggttg aacatgttca cgattagaaa
     1921 tatgaacctc tatgagcatt ggtttatctt ctaagataac tttcatgtct tcacttagag
     1981 tcgctggatt gtcaaaacta taatgactaa ttccgtaggc ctctgccaac aattggaaat
     2041 tcggttcatc gtcaaataca gattggctac gacgcttttc atagaaggat tcctgccatt
     2101 gacgaaccat tccaagagag tggttattga ttaatacaac cttaatcggt acaccatagc
     2161 cattcaaaat agccaattct tggttggtca tttggaagcc accatcaccg acaaagatga
     2221 ttacttcttt atctggattg gctaatttgg cgccgatagc tgcaggaata ccaaagccca
     2281 tggtccccat gccaccagat gttacaagtt gacgtgcatg tttgtagggg tagaattgag
     2341 cggcccacat ttgatgctga ccaacgtctg tcacgacaat ggcatcccct tgcgtcaact
     2401 gaccaattaa ttcaattgcc acctgtggct taataacctt ttcatcttca tcataccaaa
     2461 atggtgcccg acgtttgttt tccaatactt gtgccgtcca atcagcatag cctgtttcga
     2521 ctgtttctaa agcaagtaga gctttcaagg ttgccttagc atcgcctact actggaatag
     2581 ccgttttgac aactttacca atttctgcgg gatcaatatc aatatgtgct accgtcgcat
     2641 ttggcgcata ggttgttgga tttcctgtta ggcggtcatc gaatcgcgct ccaatattga
     2701 taatgtaatc agcctgatcc atagccatat tggcagcgta tgaaccgtgc atccccccca
     2761 tagccaagga taattcatgc tcaatgggca tcgcacctaa tccaaggagg gttgaaacta
     2821 ctggaatacg gtagcgctct gcaaaggcaa ctaattcttt attggcatct gcataattga
     2881 caccaccacc agccaatata actggttttt gagcttggct aagctgtttc aaaattttct
     2941 taacctgcaa accatttggt tcaatcgttg gctgataact tggtaggtgt agagttgggt
     3001 catgataaaa atcaacaact ctttcttgga tatcttttgg aacatcaata acaacgggac
     3061 ccggtctccc tgttgttgca atatggagag cttctgtgat gactctagga atatctgccg
     3121 tatcccgaat ttggtaattg tacttggtaa tcggcattgt catccccaag acatctgctt
     3181 cctgaaaagc atctttaccg ataccacgtg ttgcgacttg accagtaaaa actaaaagag
     3241 gaacactatc acccattgcg tccgcaattc cagtaatggc attggtagct cctggcccag
     3301 aggttacaat ggctacgccc acctttcctg atgatttagc gtatccttcg gcctcgtgga
     3361 ccgctccttg ttcatgacga gccaaaatat gctggattcc ctcatattga tagatagcgt
     3421 catatagagg caaaactgcc ccaccaggat aaccaaaaac aaggtctact cctagttgat
     3481 gcagggtgtc caaaatgaga taggaccccg aacgcggttg gtctagttga atttcttgca
     3541 cgaatattcc tcctctctgc aaatttgtta tctactataa tatcacaagg aagtagaatg
     3601 tcaaggaatt ttctgaaaat tcctacattt gttcgttttc ttaactattt cctaacattt
     3661 cttaagcaaa acttaagcaa atcccttgac tggcttggtt tcagcttgct ttcagaaaac
     3721 atcagtatag tagttccact aaaaaaatta aatgaggttt aggatatgta ttatgtagtg
     3781 ataccggctt accagccaga tgaaaaatta atccaactat tagaaataat gaaaaacagg
     3841 ctaaattgtc aggttatcgt tgtggatgat ggtagtactg gcgcatccaa aaatattctt
     3901 gaaatcgcca aaacctatgc aattgttctc caccatgatg tcaacaaagg aaaaggccaa
     3961 gccttgcgaa cagcctttag tttcatccaa gatttgagaa aaccaggtgt tatcgtaact
     4021 gctgacgcag atggtcaaca cactgtaaat gatattgacc gtgtcgcacg cgccgccatg
     4081 aatctaccaa gccgcctgat tcttggtgtt cgtcaattta ctaaagacat cccgctccgt
     4141 agccgatttg gtaacaaact aaccagagta ttgtttagat tgcaaactgg tgtcaatgtg
     4201 tccgataccc aaacaggctt gcgtggattc catactgact taattccatt catgttagat
     4261 attgaaggcg accgctatga gtacgagatg aacatgttaa ctcaggcaag tcaacgctat
     4321 aaaattacgg aagttccaat tcaaactatt tatatcgatg ataatgccag ttcgcatttt
     4381 cgtcccataa aagatggact gctcatttac aaaaatctct tcaaatttgc cctggcatct
     4441 tttggtggat ttctagttga ctacggaata tatgcacttg ccttattgct actcactagc
     4501 ttaccaactg ctctacgctt attggtcgca aacacgattg ctcgcatttg tagcgctacc
     4561 tgtaattttg cacttaataa gaaattagtc tttaacaatg aagagagcat cgcacgaaca
     4621 ggcgcaggct actttacact agcccttgtc ctcttccttt gcgatacagc cctcctctat
     4681 ctcttccacc aaattctagg acttaacctc tacattgtca aacttgcagt tgatatcttc
     4741 cttttcctag cctcttggtt tgttcaaaag aatattattt ttaaagaaag gaaatcattt
     4801 tcccatgaaa ttgctaaaaa aaacctttgc ctacgcagcc atcttcggaa tgcttttgac
     4861 aggaggcttt acttactcca tgttaaaaac cttcgtgctt tccgaggcta tcacaacggt
     4921 tgctgcaagt aatgccacag ccaatagcca gaacacagct acttcatcgt catcaactgc
     4981 cgcaaccact gcaactaatg tctctacaac ggatactagt tactctgatg ataatattca
     5041 gataaattta gagacgatta ccaccaataa cacaactgtt tacgtcgcag atattcaggt
     5101 cagctctcca gagtatttaa agacagctct agcccaaaat acctatggca cgaacgtaac
     5161 agctaaaact tctgaaactg cgacagcaaa caatgccatt ctagcggtta atggtgacta
     5221 ctacggagcc aattcaactg gttatgttat taagaatggc gttctctatc gcgacactgt
     5281 tcgtgacaat gctgcttacg gcgacttggc aatctacgcc gacggctcat ttgaagtcat
     5341 ctatgaaaat gaaatcactg cccaggaatt gattgacaag ggtgtcgtca acctcttagc
     5401 attcggtcca tcattggtag agaatggcga aattgtcgtc gatacttcaa cagaagtcgg
     5461 acgtgcaatg tcatccaacc ctcgttctgc tatcgggatt atcgatgaaa atcattatat
     5521 catcgtcgta gcagatggac gtacgtctga aagccaagga ctctctctat caattggcag
     5581 aagttatgaa acaatatggt gctcaaactg cctacaacct tgatggcggt ggttcatcca
     5641 ctctttactt taacggtcaa gttattaaca acccaataac aaatggaaat actatctcag
     5701 aaagggcggt gagtgacatt gtctacatcg gttactaatt caactacaag agtttcaaga
     5761 acatttatct cctacttagc aataacagct ttcctgttta ttttcagtcg aatctatgaa
     5821 agtctcagct atggcgaagt ctctgtcttt atgcattata tgttctgtat accattaata
     5881 ggcggagctg tgctactcgg tctgctgcaa cttataccta ccctttcacg tctctcttat
     5941 aatttgtgga attctggcgt tgcaactatt acagctggtt gtcttctcca aggcattatc
     6001 aacctatctg gacgttcaac taccttagat caaccttatt ggtacatcgg cgcagccttc
     6061 ctagttctat ctttagtcag tctttttatc aatccatccg caaaacaatc gtcgaaaaac
     6121 gaccacttaa tctacaaata aattgtaatg gcacttaaca ttcttaggtg ccttttactg
     6181 tagaatgaat tgttgaaaaa ttcggaaatt tccaattatt tatcagatct tttatcgttt
     6241 ctttcatatc tatgatataa tgggtaaaat aagataggga gaagaaaatt atgaccgata
     6301 caaacaaact taaagacttt cgacatcgca gctctgttta cgattcgatg gtcaaatcgc
     6361 ccaaccgtgc tatgttgcgt gccacaggca tgaccgatga tagctttgaa aagcctattg
     6421 ttggggttat ttcgacatgg gctgaaaaca caccctgtaa catccacctt catgactttg
     6481 gaaaattagc caaagaaggg gtaaaagatg caggtgcctg gcctgtccaa tacggtacca
     6541 tcaccgttgc agacgggatt gccatgggaa ctcctggtat gcgcttctcc ttgacttcac
     6601 gtgatatcat tgcagattct attgaggcag ctatgggagg acacaacgtg gatgcctttg
     6661 tcgcaatcgg tggctgtgat aaaaacatgc ctggttccat gattgccatc gccaacatgg
     6721 atatccctgc tgtttttgcc tacggcggaa ccattgcgcc aggtaatctc aatggtaaag
     6781 acatcgactt ggtatccgtt tttgaaggaa tcggaaaatg gaacaacggt gacttgaccg
     6841 ctgaagaggt tcgtcaaatc gagtgtaatg cctgtcctgg acctggtggt tgcggtggta
     6901 tgtacactgc caataccatg gctactgcca ttgaggtaat gggcatgtct attcctggct
     6961 cttcttctca ccctgctgaa tcaccagaga aaaaagcaga tattgaagaa gcaggtcggg
     7021 ccgttgtccg catgttagaa ctaggtatca aaccatctga cattatgacg cgtgaagcct
     7081 ttgaagatgc cattacagtc accatggcac tcggtggctc caccaacgct actctgcact
     7141 tgctggccat tgcccatgct gctaatgttg aattaacact ggaagatttc aatgatttcc
     7201 aagaacgcgt gcctcacttg gcagacctga aaccatcagg aaaatacgtt ttccaagacc
     7261 tctacaatgt cggcggcgtc ccagctgtca tgaaatactt gctcaaaaac ggcttccttc
     7321 atggcgaccg catcacatgt acaggtaaaa ccgttgctga aaacctagag aattttgcag
     7381 acttgactcc aggtcaagat gtcatcatgc cacttgaaaa tccaaaacgc gcagacggtc
     7441 cactcatcat cctaaaaggt aaccttgctc ctgaaggtgc cgttgccaag gtttctggtg
     7501 taaaagtccg caaccataca ggtccagcca aggtctttga ctcagaagaa gaggcgatcg
     7561 aagcagtctt gactgacgaa atcgtagatg gcgatgtagt cgttgtccgc tatgttggac
     7621 caaaaggcgg tcctggtatg cctgaaatgc tatccctttc ttctatgatt gtcggaaaag
     7681 gccaaggtga caaggtagcc cttctaacag acggtcgctt ctctggtggt acctatggac
     7741 tggttgttgg acacatcgca cctgaagctc aggatggtgg tccaattgcc tacctccgta
     7801 caggtgattt ggtgacggtt gatcaagata ccaaagaaat caccatgcac gtttccgacc
     7861 aagaaatcga agaccgcaag aaaacgacgg tcattccacc actctactct cgtggtgtcc
     7921 tcggaaaata cgcccacact gtatcctcgg cctctaaagg agctgttaca gacttctgga
     7981 gaccagaacg tacggggaaa aaataaggaa ctcaacgacc ctgtcctcgc ggcagggttt
     8041 tccaaaagga gaaaaaatga aactaaacgt cgaactctcc cgctttcgtg ttaaagaagg
     8101 caaaaccgtt caagtggatg aatggatggc ctttctcaac gagcacatgg aagacaccct
     8161 tctcactctt gaaggagaga aaatgtatgt cgaaaccatc ttccgtgagg tattggacgg
     8221 acgcgaatac ctttactggt actctgtcca agccgaagga agaattgaag tcgaagattc
     8281 agaatcctat atcgacaaaa aacatttaga gtattgggaa gaatgcatcg acccgagcta
     8341 tggtatggtc ggtttggatc cgcaagttat catgattcat aaaactatct atgaaaccat
     8401 ggaagaatta gacagacaat acgatgaaac atttaaaaaa tgagctgctc catgcaactc
     8461 attttctttt tatttaggac tgataccaag cgtaattcga cctaagcgac taattcttgt
     8521 cattttccaa gcaggagacc agacaatctc tacctgtgca tcgtttatac catcaatcgt
     8581 tttcagggct gcaaccaatt caccaggcat agtatccgca caagaacaac ctgaatctgt
     8641 aaaggtcata acaaccttac aaaaaccagc ttcatctaaa tgaatctcat aaatcaaacc
     8701 caaattgtag atgtctagtt caatttcagg atcgtaaatg gattccaaaa cctctaccaa
     8761 ctgttctgat acagttaacg cacgttcatt aatctgaata tcttctctca tcaagttcaa
     8821 acactcctaa aatatttata agaagtattg tatcataatt aaacaaaata tacctatgct
     8881 atcaaatatt tttatttaga ttttatagag cacgaacttc tgaatcctca ccttaaccga
     8941 tagaaaaaat atatttgtgc ccttgaggca aactatgata gaaaccatga cctgcataaa
     9001 tctgaaatac ttgcattgta tcactagact catctttata gatatgttga taataaggat
     9061 tatccgcaaa aactgtcttt agatagtcgt tctccacagg tctggcagtc ccttctacac
     9121 gcactacctg gataaggtac ccttcctcag aaatagccgt catcgcaact ctttgatccc
     9181 ccatcaattg atcatagaaa tgcgtttctg ggcttgtcat aaagaaaatc ccttcttcgt
     9241 ttgctgcagt gatatgagca tgacgagcat gtggatttcc atactcatcc agagtagcaa
     9301 aaactccaac cttcatatct tccaaaatat gcataatatc ttttagttcc atggtatcac
     9361 cccttgaaaa tacctatttt ctaaagtata tcaaaaacaa gcgaaaatgt taagtggaat
     9421 attgatagaa atggtgaaag tgtttcatga ataactccct actgacggaa ccatttaaga
     9481 aggcagctat gtttgcttca ttttcaacct tcaaaggttc ctcaaccctt ttgatttcca
     9541 attggtatgg atttgactac tgaaccgatt atgacaacaa tcaaaatccg tcgttactat
     9601 aaaaactacc taattataaa aaagcgactc ttgagtgctt tgaaacgttg atatttcgcg
     9661 gtatattaac gtttactaaa ctggcttgct ttacgtgctt tcttaagacc tggttttggg
     9721 tgacagaagt aaaatgccta taaaagatta ttaaatcaag ctttcaaagt ccattttcat
     9781 cacatttaac cacttataac tcaatcatac aaacaatgga ttaccattag attaccaaaa
     9841 atctgttttc ggagcttatg tgattgagga acaacattac tgacaatctc tatcttcaca
     9901 atttacttta ttaataaatg tacacaattc tcaatctcag taatcaactg ctcttgggtg
     9961 ttgaaatcat cgagagcatt ataaatctct cttatacgat ctgaaaagtt tatatttttt
    10021 gctctttcac ttatttcaga aatacttagc tcaactcctt tttgattgta ttgaaaccaa
    10081 attggtaaag tcaattttga catttttaag aaatgctcta aattttcttg tttagaataa
    10141 tgcacgatat ttaacaaaag aacattataa tttaaataag tagttaagct agaaaaaaat
    10201 gggaaagcgt tgaaagtgta agaagtcaga aaaacatcca tagcttgact gaaatatccg
    10261 tagattataa atcgtaaaag ttctgcatca ttcatatttt tagggaacat atctgaatac
    10321 tcaattcgat attgttttaa acggtatctt attgtttctg tgggatcatc ataatcaaaa
    10381 agcaatattt gctgtaaata ttcatgtaca accacagtat ctatatcctc gtact
//
LOCUS       DAZQCJ010000065        10428 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000065 DAZQCJ010000000
VERSION     DAZQCJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10428
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.066"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(333..971)
                     /locus_tag="ACHU66_001476"
     CDS             complement(333..971)
                     /locus_tag="ACHU66_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HFR3389752.1"
                     /translation="MRWGIVVEQLTVLYQNLKRRYGEFHWWNDENPIKDLVSMILIQQ
                     TTEANAKRALEQLEGRLTIHSLLEMPVEDLQECIRPAGFFKQKSLYIRSVVEWANQFD
                     GDFSRLDRVETAVLRKELLSLKGVGNETADVILLYLCRRSIFVADQYALRLFNRLGLS
                     QSQDYLSLRQEFTEQIKDWSVKDAQELHALIDEHGKQFRLTKGQLDESWLMI"
     gene            complement(1019..1174)
                     /locus_tag="ACHU66_001477"
     CDS             complement(1019..1174)
                     /locus_tag="ACHU66_001477"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389753.1"
                     /translation="MLVKGISQGQAPEIFLKEAAAFATANCLNYFPEVEAEQFEAIVD
                     TIVLEEL"
     gene            complement(1229..1939)
                     /locus_tag="ACHU66_001478"
     CDS             complement(1229..1939)
                     /locus_tag="ACHU66_001478"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFR3389754.1"
                     /translation="MIHLICPNPALDRTLLVEKIEKNIPLRPTEVRDYPGGKSFNVAY
                     ALRENGVTDYTIHTILGGQIGRYIQELNADGGNALRVVENSQNTRTCNIYVETSTGDV
                     VLFYEKGFELTEDLLNQFTQQIENSLQAGDILVFSGSLMKGVSDDYIQRFIEKYPEVL
                     TIVDTSGPALRAAYQARPALIKINNEELKDIYPELDEESPEAILGILKEVTPHENIII
                     TMGDKGSLAKIGQRFFRI"
     gene            complement(2041..2667)
                     /locus_tag="ACHU66_001479"
     CDS             complement(2041..2667)
                     /locus_tag="ACHU66_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFR3389755.1"
                     /translation="MKIAIIAANGQAGQTIAKEAVERGHQVTAIVRSENKSVAQEVIQ
                     KDAFALTKEDLAGFDVVVNAFGAWTPETLPDHARLAEHLATILAGSPTRLLVVGGAGS
                     LYLDESQTAMLKDTPDFPAEYLPIAEAMGTGLDLYRQATAVNWTYISPAADFDAEGQK
                     SGAYTLAGEVFQVNAQGESYISYADYAVALVDIAEKGGYQQERISVLA"
     gene            complement(2906..3325)
                     /locus_tag="ACHU66_001480"
     CDS             complement(2906..3325)
                     /locus_tag="ACHU66_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFR3389756.1"
                     /translation="MQISSRFTTASHILVLLALEGEKEKQTSTSIAGSVGVNPVIIRN
                     ILSQLKEAGLVEVARGVGGARLAQGPDQITLLHVYQAVELFGEKGQLFAFHEQPNPSC
                     QVGRNIHPLLDSRLENAQSALENELAKTRLADLLAEL"
     gene            complement(3368..4435)
                     /gene="dinB"
                     /locus_tag="ACHU66_001481"
     CDS             complement(3368..4435)
                     /gene="dinB"
                     /locus_tag="ACHU66_001481"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR3389757.1"
                     /translation="MLIFPLINDLSRKIIHVDMDAFFAAIEVRDNPALKGKPVIIGAD
                     PRLSGGRGVVSTCNYEARAFGIHSAMSSKEAYECCPQAIFISGNYEKYQEVGRQVREI
                     FHRYTDLVEPMSIDEAYLDVTENKLGISSAVKIAKLIQYDIWNELHLTASAGVSYNKF
                     LAKIASDMEKPYGLTLILPEDAVGILASLPVEKFHGVGKKTVERLHEMGVYTGQDLLD
                     VPEMVLIDRFGRFGFDLYRKARGISNSPVKVDRVRKSIGKERTYRKLLYREEDVLKEL
                     ISLCQRVAASLKRNGKKGRTIVLKVRYGDFSTLTKRHSLEVYTDQTETIEKEVRQLIE
                     EIGKIEKGIRLLGVTVTNFQT"
     gene            4750..7095
                     /gene="pflB"
                     /locus_tag="ACHU66_001482"
     CDS             4750..7095
                     /gene="pflB"
                     /locus_tag="ACHU66_001482"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR3389758.1"
                     /translation="MSTKVKTKNVAEDIFAQAWEGFKGTDWQDKASVTRFVQANYTPY
                     DGDESFLAGPTERSLHIKKIIEETKAGYEDTRFPMDVDRATSIADIPAGFIDKENELI
                     FGIQNDELFKLNFMPRGGIRMAETTLIENGYTPDPLLHEIYTKHATTVNDGIFRAYTA
                     DIRRSRHSHHVSGLPDAYSRGRIIGMYARLALYGADYLMEEKVADWNAITEIDEESIR
                     LREEINMQYQALQQVVRLGDHYGVDVRKPAMNTKEAIQWTNIAFMAVCRVINGAATSL
                     GRVPIVLDIYAERDLARGTFTESEIQEFVDDFVLKLRTVKFARTKAFDEIYSGDPTFL
                     TTSMAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWSDQLPYSFRRYCMAMSH
                     KHSSIQYEGVTTMAKDGYGEMSCISCCVSPLDPESEDQRHNIQYFGARVNVLKALLSS
                     WNNGYDDVHKDYKVFDGVEPNTSEVFDYDQVVKNFEIALDWLTDTYVDAMNIIHYMTD
                     KYNYEAVQMAFLPTHLRANMGFGICGFANTVDSLSAIKYAQVKPIRDEDGFIYDYEVT
                     GDFPRYGEDDDRVDDIAKWLMEAFFTRLNKHKLYKNAEATVSILTITSNVAYSKQTGN
                     SPVHRGVFLNEDGSVNTTKVEFFPPGANPTSKARGGWLQNLNTLSKLNFKHANDGISL
                     TTQVSPKALGKTFDEQVNNLVTILDGYFEQGGQHVNLNVMDLNDVYEKIMAGEDVIVR
                     ISGYCVNTKYLTKEQKTELTQRVFHEVLSMDDHAAEVSGQA"
     gene            complement(7278..8069)
                     /locus_tag="ACHU66_001483"
     CDS             complement(7278..8069)
                     /locus_tag="ACHU66_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFR3389759.1"
                     /translation="MKKFSCKSVLALAGITVALSTISRVQATSHVVMAGDSMFSIAEQ
                     YGVDPYQLAEVNAMEIDGLITPGQRLDLPAGSNEGVSVAGQTDYVVQDGDSFYGIADA
                     FSLDINKLLEQNQLTLDANIHPGQILKLTETIWNQTVDTAADSYYLPGYEYEPGINYP
                     VGQCTWGVQKVAPWAGDWWGDAASWGANAARDGFRTGTTPEVGAIISWNDGALGHVAY
                     VTAVNEDGQIQVLEANYRGQQWVDNFRGWFNPTDTIGEITYIYNN"
     gene            complement(8151..8702)
                     /locus_tag="ACHU66_001484"
     CDS             complement(8151..8702)
                     /locus_tag="ACHU66_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HFR3389760.1"
                     /translation="MRYVLLLRGINVGGRNKIVMAELRQAVADLGYDKVETYINSGNL
                     FFDSTKNRGDIVAEFQTFFTERYPFVEQFALLDTQDYQAEIDQLPAWWQEELARKDVL
                     FFTEGLDKQAMVDYIQSLHLGDEVLHFSDYAIYWGKYTEGSYLQTAYHKQLAKSPFYK
                     QVTIRNGKTFSVISNYLENEIKR"
     gene            complement(8699..9640)
                     /locus_tag="ACHU66_001485"
     CDS             complement(8699..9640)
                     /locus_tag="ACHU66_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFR3389761.1"
                     /translation="MRRVILDKVSEQIEQGVYPGASLAFFSEGQWQEYYLGTQDGHTP
                     VEAGLTYDLASVSKVVGVGTLATFLVNSGALELDRTLQSYYPAFADSEVTIRQLLTHT
                     SGIDPFIPNRDSLDADQLKEAILKIAVTDKKAFLYTDINFLLLGFLLEELGRDSLDQL
                     ISRDVLEPFGMSQTSFGPVSQAVPTVRGVKAGVVHDPKARVLGLHAGSAGLFSTVKDL
                     EIFLEHYLMADFAANLTQDYGFDVKRKRSLAWDKKGDWLSHTGYTGTFIMYNRPLQKA
                     AIFLSNRTFDKDERAQWKLDRNQVMALIRQVLEEEGQ"
     gene            complement(9637..10383)
                     /locus_tag="ACHU66_001486"
     CDS             complement(9637..10383)
                     /locus_tag="ACHU66_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HFR3389762.1"
                     /translation="MKCIVLRKNEAIVPVLRINNRAINQGFLEKNLGMKTKLEDGPFA
                     DFGDRTNPETKLVLTESPGNRTRAVEGLKKLNKIVIKVDNASEIEALLAQGSQYSKLY
                     QGKNGYGFEAVSPEGDTFLLHAEASVDDLKAILPPVPFKVQDDFSGLTAFTVESVWIN
                     TPQASINQDYYEAILPQQAFLRFIGAEGEDLLTPAEQVWDLEGLRFPVEQDFDWAHLE
                     SRLEGPFFKDKKASFIQTVDPSGIELWFEK"
BASE COUNT         3064 a         2493 c         1921 g         2950 t
ORIGIN      
        1 catccttagc agtctataat cattttatct tggaaaataa ctccaattaa tataaaaaaa
       61 gagcaaccaa ctgaaccgct ggttttactc ttttatagtc gtttcgtttg cttttagtac
      121 aaggcaacga tatgcaggct acttcaactg tccagtggag tgtttaaact cggaaataag
      181 gaaatgagac ttcctcgccc ttcagtaata aaaaatacac taattgatac tcaatgaaaa
      241 tcaaaatcaa gctaggcgac gcaaatgcag atagaactga ggttcatcaa atcatgtcaa
      301 caacgtctga ttttgatttt cgaagagtat gactatatca tcaaccatga ttcatcaagc
      361 tgcccctttg ttaacctgaa ctgcttgcca tgttcatcaa tcagtgcatg aagctcctga
      421 gcgtctttta ccgaccaatc ttttatctgc tctgtaaatt cctgtctcaa actcaaataa
      481 tcctgagact ggctaagtcc taaacgatta aatagccgaa gagcatattg atccgccaca
      541 aaaatacttc ttcgacacaa atagagcaaa ataacatccg cagtttcatt gccaaccccc
      601 ttcaagctca gcagctcttt tcgcagtact gctgtctcaa cacgatctag gcgagaaaaa
      661 tctccatcga actgattggc ccactcaacc actgaccgaa tgtagagtga tttttgcttg
      721 aagaatcctg ctgggcgaat gcattcttgt aaatcctcca caggcatctc taaaagcgaa
      781 tgaattgtca accgtccctc caactgttct aaagcacgct tggcatttgc ttccgttgtt
      841 tgctgaataa gaatcataga taccaaatcc tttataggat tttcatcatt ccaccagtga
      901 aattcaccat atcgccgttt taaattttgg taaagaaccg tcaactgttc cactactatc
      961 ccccatctca ccttgtttct ccttgccatt atagcacgat gagtaaaggg atacaatact
     1021 acagttcctc tagtactatt gtatctacaa tcgcttcaaa ctgctctgct tcaacctctg
     1081 gaaaataatt caaacaattg gctgttgcaa aggctgccgc ttctttcaaa aagatttctg
     1141 gggcttgacc ttgactgatt cctttgacta gcaagcccag gtagaaatcc cctgcggcaa
     1201 taggattacg cgtttctttc tttggagact aaatacggaa gaagcgctga ccaatcttag
     1261 ctagactccc cttgtctccc atggtaatga tgatattctc atgaggagtc acttctttca
     1321 ggatacctaa aatagcttca ggactttctt catccagctc tggataaata tctttcagct
     1381 cttcattatt gattttgata agggccggtc tagcttgata agccgcacgt aaagctggtc
     1441 cactggtgtc cactatggtc agaacttctg ggtatttttc aataaaccgc tgaatataat
     1501 catccgacac ccccttcatc agactacctg aaaagactag gatgtcgcca gcttgcaggc
     1561 tattttcaat ctgctgagtg aattggttga gcaaatcctc tgttagttca aaaccttttt
     1621 cataaaatag tactacatcg cctgtactgg tttcaacata gatattacat gtccgagtat
     1681 tctggctatt ttcaaccacg cgcagggcat ttccaccatc agcattaagc tcctgaatat
     1741 agcgaccaat ctgcccacct aaaatggtat ggatagtgta gtctgtcact ccattttctc
     1801 gaagtgcata ggctacgttg aaactttttc caccaggata atccctcact tccgtcggtc
     1861 tcaggggaat attcttttca atcttttcca caagaagcgt gcggtctagg gctggattgg
     1921 gacaaatcaa gtgaatcatg gggttctcct tttcttttta tttatattat tatactatat
     1981 gcaagtaaaa aataaaaaga taattcctcg atggagctat cttttagacc atttaatttt
     2041 ttatgcaagt acggaaattc gttcctgctg gtagccacct ttttccgcaa tgtcaaccaa
     2101 agccacagca tagtcggcat aactaatata gctttcacct tgggcattga cttggaaaac
     2161 ctcgccagcc aaagtgtagg cacctgattt ttgaccttct gcatcaaaat ctgctgctgg
     2221 gctgatatag gtccagttga ccgctgtcgc ctgacgatag aggtctagac cagtacccat
     2281 agcctcagcg attggcaggt attccgcagg gaaatctggt gtatctttca gcatagctgt
     2341 ctggctctcg tctaaataaa gactgcctgc accaccaact actaaaagac gagttggact
     2401 accagcaagt atggtcgcta gatgctcagc aagacgagcg tgatccggca aggtttctgg
     2461 tgtccaagca ccaaaagcat taactactac atcaaagcct gccaaatctt ctttggtaag
     2521 agcaaaagca tccttctgga ttacttcttg ggctacgctc ttgttttcag aacggacaat
     2581 agccgtcacc tgatgaccac gttctactgc ttcctttgca atagtttgac ctgcttgacc
     2641 attcgctgca ataatagcaa ttttcatatt gatacctcac ttgtttcgtt gtaacaactt
     2701 cgtttacaac gtaagtataa attattttgg ttgtaacgtc aattgttaca gcttgaaaat
     2761 taaaaaaatc atcacaagga tgattcacta actagtcttt tagtgctagg gcaacactct
     2821 acagactact tcaacagtcc agtggagtgt ttgaagtagg aaatagggaa acaaagtttc
     2881 tccttacgtt gacctaataa aacgactata gttccgccaa gagatcagca agccttgtct
     2941 tagcaagttc attttccaaa gccgactgag cattttccaa acggctatct agcagaggat
     3001 gaatattgcg acctacttgg caggaaggat tcggttgctc atgaaaagcg aataattgac
     3061 ctttttctcc aaataactcc acagcctgat agacatgcaa aagtgtaatc tggtctggac
     3121 cctgtgccaa gcgagcaccc ccaacaccac gcgctacttc taccagacca gcctccttga
     3181 gttgggacaa aatatttcgg ataatgacag gattaacccc gacactgcct gcaatgctag
     3241 tactggtctg tttttctttt tctccttcca gagccaagag aaccaagata tggctggcag
     3301 tggtaaatct acttgaaatt tgcatctctt ctcctttttt tcagtctatc atagactgaa
     3361 acagaactca agtttgaaaa ttagtcacgg tcacacccag caaacgaatc cctttttcaa
     3421 tcttcccaat ttcttcaatt agttgacgaa cctctttctc aatcgtttcc gtttggtccg
     3481 tatacacctc tagactatga cgtttcgtca aggtagaaaa atcaccataa cgtaccttta
     3541 aaacaattgt tcgccccttt tttccatttc gcttcaaact ggctgcgacc cgttggcaga
     3601 gactgattag ctctttcagg acatcctcct cccgataaag aagtttgcga taggttcttt
     3661 cctttccaat tgacttacga acacggtcca ctttaacggg cgaatttgaa atccccctcg
     3721 ctttccgata aaggtcgaag ccaaaacggc caaatctatc tatcaaaacc atttctggta
     3781 catctagtaa atcttgcccc gtgtagaccc ccatctcatg caggcgttcc accgtcttct
     3841 tccccacacc gtgaaatttt tcaacaggca gggaggcgag aataccaaca gcatcttcag
     3901 gtagaatcaa ggtcaaaccg tacggttttt ccatatcaga tgcaattttt gccaaaaatt
     3961 tattatagga aacgcctgcg gaagctgtca gatgcaattc attccaaata tcgtattgga
     4021 taagcttggc aatcttaacc gcagagctga tgcccaactt attttctgtt acatccaagt
     4081 aagcctcatc aatagacatg ggctccacca aatcagtata gcgatgaaaa atttctcgaa
     4141 cctgtcgccc aacttcctga tatttttcat aattgccaga aatgaaaatc gcctgaggac
     4201 aacactcata ggcctctttt gaggacatag ccgagtgaat cccaaaggca cgcgcctcat
     4261 aattacaggt agataccact cctctaccgc cagatagtct tgggtcagcc ccaatgataa
     4321 caggtttgcc cttcaaagcg ggattatccc tgacctcaat ggcagcaaaa aaagcatcca
     4381 tatctacatg aataattttt ctagataaat cattgattaa gggaaaaatt aacatgactg
     4441 cctcttcctg tttacaagtt tgactcgcta tattataccg catttcttag gaagtttcag
     4501 cctttagatt cgttgaacca gccatcttat caatacaata aacgacagct tatcacctta
     4561 ttacaaatac aagcagaaat ttattttgaa aataccaact ctatctttca attttttcat
     4621 attccttgaa aggtttttca tttattttca agtatttttg tcttgtagaa agtgttttca
     4681 aatggtataa taatgacatg ttggtatcga tagcaagtcg atacccaaaa gcataaaagg
     4741 agaaccctca tgtcaacaaa agttaaaaca aaaaatgttg ctgaagacat tttcgcccaa
     4801 gcctgggaag gctttaaagg tacagattgg caagataagg caagtgtaac acgcttcgtt
     4861 caagccaact acactccata cgatggagat gaaagcttcc tcgcaggccc aactgagcgt
     4921 tcacttcata tcaaaaaaat catcgaagaa acaaaagctg gctacgaaga cactcgcttc
     4981 ccaatggacg tagatcgtgc tacttctatc gctgatattc cagcaggttt catcgataaa
     5041 gagaacgaac tcatcttcgg tatccaaaac gatgagctct tcaaactcaa tttcatgcca
     5101 cgtggtggta ttcgtatggc tgaaacaact cttatcgaaa acggctatac tccagaccca
     5161 ctccttcatg aaatctatac aaaacacgct acaactgtaa atgacggtat cttccgtgcc
     5221 tacacagctg acatccgccg ttctcgccac tcacaccacg tttctggtct tccagatgca
     5281 tactcacgtg gtcgtatcat cggtatgtac gctcgtttgg cactttacgg tgcagactac
     5341 ttgatggaag aaaaagtagc tgactggaat gcgattactg aaatcgacga agaatcaatc
     5401 cgtcttcgcg aagaaatcaa catgcagtac caagcattgc aacaagttgt acgcttaggt
     5461 gatcactatg gtgttgatgt tcgcaaacct gcaatgaaca ctaaagaagc tatccaatgg
     5521 acaaacattg ctttcatggc tgtatgtcgt gttatcaacg gtgcagcaac ttctcttggt
     5581 cgtgtgccga tcgttcttga catctatgca gagcgtgact tagctcgtgg tacatttact
     5641 gaatctgaaa tccaagaatt tgttgatgac ttcgtattga aacttcgtac agtgaaattc
     5701 gctcgtacaa aagccttcga cgaaatctac tctggtgacc caacattctt gacaacatct
     5761 atggcaggta tgggtaacga tggtcgtcac cgtgttacta agatggacta ccgtttcttg
     5821 aacacacttg acaacatcgg taactctcca gaaccaaact tgacagttct ttggtcagac
     5881 caacttccat attcattccg tcgctactgt atggcaatga gccacaaaca ctcttctatc
     5941 caatacgaag gtgtgacaac aatggcgaaa gatggttatg gcgaaatgtc atgtatctct
     6001 tgctgtgtat ctccacttga tccagaaagc gaagaccaac gccacaacat tcagtacttc
     6061 ggtgctcgcg taaacgtcct taaagctctt ctttcaagct ggaacaacgg ttacgatgac
     6121 gttcacaaag attacaaagt attcgacggt gttgaaccaa acacatctga agtatttgac
     6181 tacgaccaag tagttaagaa ctttgaaatc gcccttgact ggttgactga tacatatgtt
     6241 gatgccatga atatcatcca ctacatgaca gataaataca actacgaagc agttcaaatg
     6301 gccttcttgc caactcacct tcgtgctaac atgggcttcg gtatctgtgg tttcgcaaac
     6361 actgttgact cattgtcagc aatcaaatat gcacaagtta aaccaatccg tgacgaagat
     6421 ggcttcatct acgactacga agtaactggt gacttcccac gctacggtga agacgatgac
     6481 cgtgtagatg atattgcgaa atggctcatg gaagcattct tcactcgttt gaacaaacat
     6541 aaattgtaca agaatgctga agctacagta tctatcttga caatcacttc taacgttgct
     6601 tactctaaac aaacaggtaa ctctccagtt caccgtggtg tattcctcaa tgaagatggt
     6661 tctgtgaaca ctactaaggt agaattcttc ccaccaggtg ctaacccaac ttctaaagct
     6721 cgtggtggtt ggttgcaaaa cttgaatacc ctttctaaac tcaacttcaa acatgccaac
     6781 gacggtatct cattgactac tcaagtttct ccaaaagcgc ttggcaaaac attcgatgag
     6841 caagtaaaca acttggtaac aatccttgat ggttactttg aacaaggtgg tcaacacgtt
     6901 aacttgaacg ttatggactt gaacgacgtt tatgaaaaaa tcatggctgg tgaagatgtt
     6961 atcgttcgta tctctggata ctgcgtaaac actaaatacc tcactaaaga gcaaaaaact
     7021 gaattgactc aacgtgtctt ccacgaagtt ctttcaatgg acgaccacgc tgctgaagtt
     7081 tcaggtcaag cttaatcaag caaacacaaa aggacttgca atgcaggtcc tttttactat
     7141 gtcgaaaaat ctactgagtg ttgcggaagg atagtaaatc ttcggaagga tgcaccgcag
     7201 attttcggaa ggatacagaa ccagttcata aaaaaatccc tttcgaaatt ggattcgaaa
     7261 agggatgatt atttatccta gttattatag atataggtaa tttctccgat ggtatccgtc
     7321 ggattaaacc agccgcggaa attatccacc cactgttggc cacgatagtt ggcttcgagg
     7381 acttgaattt gtccgtcctc gttaacagct gtcacatagg caacgtgtcc cagagcacca
     7441 tcattccaag aaataatggc tccaacttca ggtgttgttc ctgtacggaa accatcacgc
     7501 gccgcattgg ctccccaaga agccgcatcg ccccaccagt cacctgccca aggggcaact
     7561 ttctgtactc cccatgtaca ttgaccaaca gggtagttaa tgcctggttc atactcatag
     7621 cctggtaaat agtaactgtc agcagccgta tcaaccgtct gattccaaat tgtttctgtt
     7681 aacttgagaa tttgacctgg atgaatattg gcatctaatg tcaactgatt ttgttccagc
     7741 aatttattga tgtccaatga aaaagcatct gcaatcccgt aaaacgaatc cccatcttga
     7801 acaacatagt ctgtttgacc cgcaactgag actccttcat tagaaccagc tggaagatcc
     7861 aaacgttgac ctggtgtaat cagtccgtct atttccatcg cattcacttc agccaattgg
     7921 tagggatcaa ccccatattg ctccgcaata gagaacatgg aatcccctgc cattactaca
     7981 tgactagtag cttgaacacg agatatggtt gataacgcaa ccgtaatccc tgcaagagct
     8041 agaacagatt tgcaactaaa ctttttcata tgacacctcc tcatcatgat ttcattttac
     8101 caagattttg acgggaaaag atgtcgaaag cattacaaat aaatgacgat ttatcgtttt
     8161 atttcattct ctaaatagtt tgatataact gagaaagtct tcccattccg aatggtcact
     8221 tgtttgtaaa aaggtgactt agccaactgc ttgtggtagg ctgtttgtag atagctacct
     8281 tcagtgtatt tgccccaata aatagcatag tcggagaaat gtagaacttc atcacctaga
     8341 tgcaaggact gaatatagtc caccatagcc tgcttatcca atccttcggt gaaaaagaga
     8401 acatcctttc tcgccaattc ttcttgccac caagctggaa gctgatcgat ttctgcttga
     8461 taatcttggg tgtctagtag agcaaattgc tccacaaagg ggtatctttc tgtgaaaaaa
     8521 gtttgaaatt cagcaacaat gtcccctcta ttcttggttg aatcaaagaa gagattaccg
     8581 ctattaatgt aggtttcaac cttgtcatag cccaaatctg ccaccgcttg acgcaattca
     8641 gccatgacga ttttgttacg accaccgaca ttgatccctc tcagcagaag aacatatctc
     8701 attgcccctc ttcctccaag acctgacgaa tcaaggccat aacctggttt ctatccaact
     8761 tccactgggc ccgctcatcc ttgtcaaaag tacgattcga gagaaaaatc gcagctttct
     8821 gcaagggacg gttatacatg ataaaggtcc ccgtatagcc tgtgtgcgac aaccagtccc
     8881 ccttcttatc ccaagccaga gaccgcttac gcttaacatc aaaaccgtaa tcctgcgtta
     8941 aattcgccgc aaagtcagcc atcagatagt gttccaaaaa gatttccaaa tctttgacgg
     9001 tcgaaaagag acctgcactt cccgcgtgga ggcccaagac acgcgccttg ggatcatgca
     9061 ccacacctgc tttgacgcca cgaaccgtcg gcaccgcctg actgactggt ccaaaggagg
     9121 tttgggacat accaaagggc tctagaacgt ctcgactaat cagctggtct agactgtctc
     9181 tacccaattc ctccaacaaa aagcccagta agagaaaatt aatatccgtg tagaggaaag
     9241 ctttcttatc cgttacagca atcttcaaaa tcgcttcctt gagctggtcg gcatccagac
     9301 tatccctatt tggaataaag ggatcaatac cactagtatg ggtcaataat tgccgaatgg
     9361 tgacctcact atccgcaaaa gctggataat aagactgcaa ggtcctatct agctccaaag
     9421 ctccgctatt aaccagaaaa gtcgcaagag tccccacacc aaccaccttg gaaacagacg
     9481 ccaaatcata ggtcaaaccc gcctcaactg gcgtatgccc atcctgagtc cctagatagt
     9541 actcctgcca ctgaccctca gaaaaaaaag ccagactggc accaggataa actccttgct
     9601 caatctgctc tgaaaccttg tctaaaatta ctcgtctcat ttttcgaacc acaattcaat
     9661 accacttggg tcaactgttt ggataaagct tgctttttta tctttaaaga atggtccttc
     9721 caaccggctt tccaaatgtg cccaatcaaa gtcctgctcg acgggaaaac gcaatccttc
     9781 taaatcccaa acttgttctg caggagttaa taaatcctca ccttctgctc caataaaacg
     9841 caaaaaggct tgttgaggta ggatagcctc ataatagtct tggttaatac tagcttgtgg
     9901 ggtattaatc cagacagact ctactgtaaa agcagtcaat ccagaaaagt catcctgcac
     9961 cttaaatgga acaggtggaa ggattgcttt taggtcatca acagacgctt ctgcgtgtaa
    10021 aaggaaggtg tctccttctg gcgacacagc ttcaaaacca tagccatttt ttccttggta
    10081 gagcttacta tactgtgacc cttgcgccag taaagcttca atttcagagg cattatctac
    10141 ttttatcaca attttattca attttttaag cccttcgaca gcacgggtgc gattaccagg
    10201 agattctgtc aaaacgagct tcgtttctgg atttgttcta tctccaaaat cagcaaaagg
    10261 accatcttct agcttggttt tcattcctag atttttttct aaaaatcctt gattaatagc
    10321 cctattatta atacgtaaaa cagggactat agcttcattt tttctcaaca cgatacattt
    10381 cactcctcag tcattctaca atattgtata aaaaatttcg tcatttta
//
LOCUS       DAZQCJ010000066        10403 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000066 DAZQCJ010000000
VERSION     DAZQCJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10403
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.067"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(96..287)
                     /locus_tag="ACHU66_001487"
                     /pseudo
     CDS             complement(96..287)
                     /locus_tag="ACHU66_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
     gene            complement(545..1480)
                     /gene="dapA"
                     /locus_tag="ACHU66_001488"
     CDS             complement(545..1480)
                     /gene="dapA"
                     /locus_tag="ACHU66_001488"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR3389763.1"
                     /translation="MSIQDLRDVKIITAMITPFKEDGSINFEVLPELIEHLLSHHTEG
                     ILLAGTTAESPTLTHEEELELFGAVQKIVNGRVPLIAGIGTNDTRDSIEFAKEVAAFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVEMTPETMLRLAE
                     HPNIIGVKECTSLANMAYLIEHKPEDFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMYEMFTAIEQQDIRTAAAIQRKFIPKVNALFSYPSPAPVKAVLNYLGFEVGPLRLPL
                     VPCPEEDAKRIIKVVVDGDYEATKATVTGVVRPDY"
     gene            complement(1553..2629)
                     /locus_tag="ACHU66_001489"
     CDS             complement(1553..2629)
                     /locus_tag="ACHU66_001489"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR3389764.1"
                     /translation="MAYVVAVVGATGAVGAQMIKMLEESTLPIEKVRFLASARSAGKT
                     LQFKGQDIVIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGAVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLERIIVSTYQAVSGA
                     GMGAILETQAQLRSVLNDGVEPKAVEANILPSGGDKKHYPIGFNAIPQIDLFTENDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKGIAPIDEVKAAIASF
                     PGAVLEDDVANQIYPQAINAVGSRDTFVGRIRKDLDKENGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(2798..3892)
                     /locus_tag="ACHU66_001490"
     CDS             complement(2798..3892)
                     /locus_tag="ACHU66_001490"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HFR3389765.1"
                     /translation="MKVFASPSRYIQGKHVLFQGAEAIGKLGTKPLILCDDLVYGIVG
                     EKFLSYLVEEGMQVYRVAFNGEASDKEIQRVVEIGKEQASDVVIGLGGGKTIDSAKAI
                     ADLLGVPVVIAPTIASTDAPTSALSVIYSEEGAFERYIFYKKNPDLVLVDTAIICQAP
                     PRLLASGIADGLATWVEARAILQSNGTTMAGGGQTLAGIAIAQTCEQTLFEYGLQAMA
                     SCEAKVVTAALENIVEANTLLSGLGFESAGLAAAHAIHNGFTALEGDIHHLTHGEKVA
                     YGTLTQLFLENRPKEELEKYIRFYQALNLPTTLEELHLADAGYEDLLKVGQQATIEGE
                     TIHGMPFAISAEDVAEALMAVDYYVRSLDK"
     gene            complement(4013..4969)
                     /locus_tag="ACHU66_001491"
     CDS             complement(4013..4969)
                     /locus_tag="ACHU66_001491"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFR3389766.1"
                     /translation="MKQFSGLSRTIQGNHLLDTCAIDILKIGHRPLVLCAKQEYNTTV
                     ESFVKVLTCHGLIVKIVYMEEWDQNSVEECGEVGFRHRADHIIGIGNSSVAACTKAVA
                     DLLDLPAVLIPTSPFTTLPWVSVENKKRERPLFNESIQLIIADREQLLTQPTSELING
                     VVNTLLYLAAIKEVKRNHLFSLVDDILIRSFESVLLRGSLQAIEDFEARILSKEVQDV
                     LEVLSTCVTLDLPSDAWDVIQQIESKLSERREDLVDEPLKRNVVLYLLLSYLTQESEE
                     FQARLKWCQQLLLSANSSNLLSLEAVLKEIALEMGEDGSTRC"
     gene            complement(5001..5669)
                     /locus_tag="ACHU66_001492"
     CDS             complement(5001..5669)
                     /locus_tag="ACHU66_001492"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR3389767.1"
                     /translation="MEFMLDTLNIEEIRKWAEVLPLAGVTSNPTIAKKEGDIDFFERL
                     HLIRDIIGPNASLHVQVVAKDYEGILADAKKIRELAPENVYIKVPVTPAGLAAMKTLK
                     AQGYQITATAIYTVFQGLLAIEAGADYLAPYYNRMVNLNIDSNAVIAQLSEAIDRECS
                     ESKILAASFKNVEQVNQAFANGAQAITAGADIFEAAFSMPSIEKAVNDFADDWSAIHG
                     RYTI"
     gene            complement(5684..8128)
                     /locus_tag="ACHU66_001493"
     CDS             complement(5684..8128)
                     /locus_tag="ACHU66_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR3389768.1"
                     /translation="MKTVTEVAGTSIKTEYFGSLTDRMNKYREDVFDKKPYIDAERAV
                     LATKAYQEHREKPNVLKRAYMLKEILENMTLYIEEESLIAGNQASSNKDAPIFPEYTL
                     EFVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWEKNNLRSRAGVLLPEEVQVYMET
                     GFFGMEGKMNSGDAHLAVNYQKVLEQGLRGFEERVRAAKANLDLTDPASIDKYHFYDS
                     IFIIIDAVKAYANRFVALATELAEKAPTAQRRQELLEIARICAKVPYEPAETFAEAVQ
                     SVWFIQCILQIESNGHSLSYGRFDQYMYPYVNADLEAGRETEDSIVERLTNLWIKTIT
                     INKVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTV
                     RYHAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIEKGVLEEDAYDYSAIGCVET
                     AVPGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPAFGHFKDMKSFEELETAW
                     EKTLRHLTRMSVIVENAIDIALEREVPDILCSALTDDCIGRGKHLKEGGAIYDYISGL
                     QVGIANLSDSLAAIKKLVFEEGRLTPEELWHALETDYAGERGKEIQEMLIEDAPKYGN
                     DDDYADQLVTAAYDIYIDEIAKYPNTRFGRGPIGGIRYSGTSSISANVGQGRGTLATP
                     DGRNAGTPLAEGCSPSHNMDKNGPTSVLKSVAKLPTHEIVGGVLLNQKVNPQTLAKEE
                     DKQKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQLHPEKHRDLIVRVAGYSAFFNVL
                     SRATQDDIIGRTEHTL"
     gene            complement(8280..9581)
                     /locus_tag="ACHU66_001494"
     CDS             complement(8280..9581)
                     /locus_tag="ACHU66_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFR3389769.1"
                     /translation="MSKIDTQKIIVPIMKFVNMKGIIALKDGMLAILPLTVVGSIFLI
                     LGQLPFEGLNAAIASIFGAEWTEPFMQVYSGTFAIMGLISCFAIGYSYAKNSGVEPLP
                     AGVLSLSSFFILLKSSYIPVDGEPIGDAIAKVWFGGQGIIGAIIIGLVVGAIYTTFIQ
                     RHIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYIVSKMATNGGTFIEMIYDVIQV
                     PLQGLTGSLPGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKALLAADKLS
                     LENGAHIVTQQFLDSFLIMSGSGITFGIVFAMLFAAKSKQYKALGKVAAFPALFNVNE
                     PVVFGFPIVMNPVMFLPFVLVPILAAFIVYGAISIGFMQPFSGVTLPWSTPAIISGFM
                     VGGWQGAVVQIIILAVSTIVYFPFFKIQDKIAYESETKEPV"
     gene            complement(9594..9899)
                     /locus_tag="ACHU66_001495"
     CDS             complement(9594..9899)
                     /locus_tag="ACHU66_001495"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR3389770.1"
                     /translation="MVKIALFCAAGFSTGMLVNNMKIAAADAGVEVEIDAHSQGKIAD
                     YLDEIDVALLGPQVAYTLDKSKALCDEKQVPIAVIPMADYGMLDGKKVLQLALSMVS"
     gene            complement(9932..10255)
                     /locus_tag="ACHU66_001496"
     CDS             complement(9932..10255)
                     /locus_tag="ACHU66_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFR3389771.1"
                     /translation="MEMIVADQLIMGLIIYAGDAKSHAYQALSLVKAGEEERAEEELE
                     LADAALLEAHNMQTKFLAQEAGGTKSDITALFVHSQDHLMTSITEINLIKEIIDLRKE
                     LKKVG"
BASE COUNT         2989 a         2363 c         1956 g         3095 t
ORIGIN      
        1 tgcttgtttt ctccataaga ccagttcctc ccttatttgg tatctacgta ataaaaaaac
       61 ttccctaacc attgctgatt agagaaggat atttcttaaa aaatgagacc ataaaggaca
      121 gccgcaagca agcctccgat gattggacca actactggta cccaagcata gccccaatct
      181 gaatctccct tatgcttcaa tggaaggatg gcaagcatga tacgaggacc aaggtcacgc
      241 gcagggttta gggcatatcc tgtcggacca ccaagagaaa ctccgagtca aaacaaaggt
      301 tccgattgct tcgctaattg taaatgtccc ccagacattg ttcgctctct tatttcaagg
      361 ctcacgttaa aatacttcgc ttttttattt tcaagctcag ataagaatag tccactggac
      421 tattcttatc ccctaactat cctcgctttc cagtttccag gctcgggtct tctatggtca
      481 ctttcatatt tcttggtgac cagctgaaac agttccccga actgtttcac tcccccagac
      541 atttttaata atccggtctt acgactcctg tcacagtggc tttagttgct tcgtagtcgc
      601 cgtcaacgac tactttgata atgcgtttgg catcttcttc tgggcatgga accaacggca
      661 agcggagtgg accaacttca aatccaaggt aattaagaac cgccttaact ggtgcaggac
      721 ttggatatga gaagagggca ttgactttcg gaatgaactt gcgttgaata gcggctgctg
      781 ttcgaatatc ttgttgttcg atggctgtaa acatttcata catctcatca ccattcgtgt
      841 gagatgcaac agaaatcaca ccatctgctc ccaagttcat tgcatggaaa gcatcgccat
      901 cctcacctgt ataaatcaag aagtcttctg gtttatgctc aattaggtaa gccatattgg
      961 caaggcttgt acactcttta acaccgatga tatttggatg ctctgccaaa cgcaacatgg
     1021 tttctggtgt catttcgacc acaacacgcc ctggaatatt gtagataatg attggtaaat
     1081 cagaagcatc tgcaatggcc ttgaagtgct gatacattcc ctcttgagag ggtttgttgt
     1141 agtaaggcac gatagcaagc cctgccgcaa agccgccgaa tgccgccact tctttggcaa
     1201 actcaatcga gtcgcgcgta tcgtttgtac cgatacctgc aattagggga acacgaccat
     1261 tgacaatttt ttgcacagca ccaaatagtt ccagttcttc ctcgtgtgtc agggttggac
     1321 tttcagcagt tgttcctgct aataaaatcc cttctgtgtg atgggacaac agatgctcaa
     1381 tcaactcagg taaaacttca aaattaatcg agccatcttc tttgaaaggg gtaatcattg
     1441 ctgtaataat ttttacatca cgtaaatctt gaatagacat aggcttcctc tcttactgat
     1501 tgttgctaaa aaaggctgag tgcagccttt tgtgtacttt tggttaataa aattatttca
     1561 attcaaactt caattctgcc gttggacgaa ctagaccacg ctcatgaagg gtttcagcga
     1621 tttgaacaga gttccatgca gcgcctttga gcaagttgtc agaaacaacc cacatgtgaa
     1681 taccgttttc cttgtccaaa tctttacgga tgcgaccaac aaaagtatcg cgactaccaa
     1741 cagcattaat ggcttgcgga taaatttgat tagccacatc gtcttctaaa acagcccctg
     1801 ggaaggatgc aatagctgcc ttcacttcat caatcggtgc aattccctta gtttcaatgt
     1861 aaacagattc agagtgagct gacaagactg gaatacggac gcaggttgca gaaacggcaa
     1921 tgctgtcatc ttccatgatt ttcttagtct cttttgtcat cttcatctct tcgtatgtgt
     1981 agtcattttc agtgaagagg tcgatttgcg gaatagcatt gaaaccgatt ggatagtgct
     2041 tcttatcgcc accagaagga agaatattcg cttctaccgc tttgggttcc acgccatcat
     2101 tcaagacaga acgaagctga gcttgtgttt ccaagatagc tcccatacct gctccagaga
     2161 ctgcctgata ggtggatacg atgatccgct ccaagcccca tttctgacga acaggctcca
     2221 aggcaaccat catttgaatg gttgagcagt ttggacaagc aatgatacca ttgtgggcat
     2281 caagcgcgtg agcatttacc tcaggaacaa ccagaggaac atctggattt tgacggaagt
     2341 aagaggtatt gtcaactact acagctcctg cttttacagc gtaaggagcg tacttggcag
     2401 atgtagagcc accagctgag aagagggcaa tatccactcc ttcaaaggct gtttctgttg
     2461 tttcttcaat gacaatatcc tgccccttaa actgcaaggt cttacctgct gaacgggcag
     2521 aagcaaggaa acgcactttc tcgattggaa gtgttgactc ttccaacatt ttaatcattt
     2581 gggcaccaac tgcaccagtg gcaccaacaa cagctactac ataggccata gaaatctcct
     2641 ttaagtcaat tcttcaaaat tttctaaata ttttatattt aacaattata tcataatttt
     2701 ttcaaatgaa aagccctgag aaagaatttc ccaaggcaat tacttcgaac ctctattcac
     2761 cagtttagat ggacatgctc aactgtgaat gctcttctta tttatctaat gagcgaacat
     2821 agtagtcaac tgccatcaga gcttctgcaa cgtcttctgc tgaaatagca aatggcatac
     2881 cgtgaattgt ttctccttca atcgtcgctt gttggccgac tttcaataga tcttcataac
     2941 cagcatcagc caaatgtaac tcctccaaag ttgttggaag attcaaggct tgatagaaac
     3001 ggatgtattt ttccaattct tctttcggac ggttttccaa gaagagctgg gtcaaggtcc
     3061 cataagccac tttttcacca tgtgtcaaat ggtgaatgtc tccctccaag gcggtgaatc
     3121 cattgtgtat cgcatgcgcc gcagccaaac ctgcactttc aaatcctaaa ccacttagca
     3181 aggtattggc ttcaacaata ttctctaaag cagctgtcac aaccttggct tcacagctgg
     3241 ccatggcttg aagtccgtat tcaaagagtg tttgttcaca ggtttgtgca atagcgatac
     3301 cagcaagtgt ctgacctcct cctgccattg ttgttccatt gctttgtaaa atagcacgcg
     3361 cttctaccca cgtcgccaag ccatctgcaa taccagaagc caaaagacgt ggcggtgctt
     3421 gacaaataat cgctgtatct accaagacaa gatctggatt tttcttatag aaaatatagc
     3481 gttcaaacgc cccttcttca gaataaatga cagataaagc agatgtcgga gcatctgttg
     3541 aagcaattgt tggagcaatg acaactggca ctcctagtaa atcagcaatc gccttggctg
     3601 agtcaatcgt cttaccacca cctagaccga ttacgacgtc actagcctgt tcttttccga
     3661 tttctacgac gcgctggatt tccttatcag atgcttctcc attaaaggca acacgataga
     3721 cttgcatccc ttcttcaacg agataggaca aaaacttctc accgacaatc ccatatacta
     3781 aatcatcaca aagaatcaaa ggctttgttc ccaattttcc gatagcttct gcaccttgga
     3841 aaaggacatg tttaccttga atatagcgag atggacttgc aaatactttc ataagaattc
     3901 ctccttgaaa taatagatat tgttatattg agcaataaac gacagttagc attgccgaaa
     3961 gtaagaggta tgagcctgca ttttaaatat tgctattgta cagcgatgcc tactagcaac
     4021 gtgtcgagcc atcttctccc atctctagtg caatttcctt taaaactgcc tccagcgaca
     4081 atagattgct agagttggca gacaaaagca actgttgaca ccacttcagc ctggcttgaa
     4141 attcttctga ttcctgtgtc aaataggaca gaagcaagta gaggacaaca tttcgtttca
     4201 atggctcatc caccaaatcc tctcttcttt cgctgagctt tgattctatt tgttgaatca
     4261 cgtcccaagc atcactaggc aggtctaatg ttacacaagt agaaagaact tctaagacat
     4321 cttgtacttc ttttgataat atccgagctt caaaatcctc tatagcttga aggctacctc
     4381 ttaataaaac gctttcaaaa cttctgataa gaatatcatc taccaagctg aatagatgat
     4441 ttctttttac ttcttttatg gctgctagat aaagaagagt attgaccact ccatttatca
     4501 actccgaggt aggttgtgtc aataattgct ccctatccgc tataattagc tggatggact
     4561 cattaaacaa tggtcgctct cttttcttat tttccactga tacccaaggt agagtggtaa
     4621 acggtgaagt cggtataaga acagctggca agtctagtaa atctgcaaca gcctttgtac
     4681 aagctgctac agaactatta ccaatgccaa taatatggtc tgcacgatgt cggaaaccaa
     4741 cttccccaca ttcttcaacc gagttctggt cccactcctc catatagact attttaacaa
     4801 taagtccatg acaagttaaa actttcacaa aactttctac agtcgtatta tactcctgtt
     4861 ttgcacataa aacaaggggg cgatgaccta ttttcaaaat gtcaatcgca caagtgtcta
     4921 aaagatgatt tccttggata gtgcgtgaaa gacctgagaa ttgtttcata gtgccccctt
     4981 attttataat atagagatgt ttagatagta taccttccat gaatggcaga ccaatcatct
     5041 gcaaaatcat ttaccgcctt ttcaatggat ggcatagaaa aggctgcttc aaatatatct
     5101 gcacctgccg taatagcctg cgccccattt gcaaatgctt ggtttacttg ctccacattt
     5161 ttgaatgacg ccgctaaaat tttactttcc gaacactccc tatcaatcgc ttctgataac
     5221 tgagcgatga ccgcgttcga atcaatattc agattgacca tgcgattgta gtacggcgct
     5281 aagtaatctg cacccgcttc aatagctaac aagccctgaa aaacggtata aatcgctgtt
     5341 gctgtaatct gataaccttg cgctttcaat gttttcatag cagccaaacc agcaggcgtt
     5401 accggaacct taatatagac attttccggt gccaattcac gaattttttt agcgtctgcc
     5461 aaaatccctt cataatcctt tgccacaact tgcacatgta gtgaagcatt tggaccgatg
     5521 atgtcacgga taaggtgcaa acgttcaaaa aagtcaatgt caccttcttt cttcgcaatc
     5581 gttgggtttg aggtcacccc cgcaaggggt agaacctcag cccattttcg tatttcctct
     5641 atatttaagg tgtccagcat aaattccata ataataacct cttttacaat gtatgttctg
     5701 tacgaccaat gatgtcgtct tgtgttgctc tcgacaatac attgaagaat gctgaataac
     5761 ctgctacacg gacaatcaaa tcacggtgtt tttcaggatg tagttgtgca tcaatcaagg
     5821 tttctcgaga gacaacattg tactgaatat gatagccatg caagcggtta aagaatgtcc
     5881 gcaagagggc aattaatttt tgtttgtctt cttctttggc aagtgtttgt ggattgacct
     5941 tctgattgag cagaacgcct ccaacaattt catgtgttgg taatttggca actgatttca
     6001 aaacggatgt cggtccattt ttatccatat tatgtgatgg agaacaacct tctgccaacg
     6061 gtgtacctgc atttcgacca tctggagttg ccaaggtacc acgcccctgt ccaacgttgg
     6121 ctgaaatgga tgatgtacct gaataacgga taccaccaat cggaccacga ccaaagcgag
     6181 tgtttggata tttggcaatc tcatcgatat aaatatcata cgctgctgtt accaattggt
     6241 ctgcataatc atcatcgtta ccatattttg gtgcgtcctc aatcaacatt tcttggattt
     6301 ccttaccacg ctcaccagca tagtctgttt ccaaagcatg ccataattct tctggtgtta
     6361 atcgtccttc ttcaaagact aattttttga ttgctgctag agaatcagat aaattggcaa
     6421 tgccaacctg cagaccagaa atgtaatcgt aaatcgctcc accttctttc aagtgcttac
     6481 cacgaccgat acagtcatct gtcagagctg agcagagaat gtctggtact tctctttcta
     6541 aagcaatgtc aattgcattt tcaacgatga cactcatccg tgttagatgg cgaagcgtct
     6601 tttcccatgc cgtttcaagc tcctcaaagg atttcatatc tttgaagtga ccaaaggctg
     6661 gtgcaaaccg tttgcctgat gcaggatcga taccatcatt catggtaatc aataaaactt
     6721 tcgggaagtt catatagctc atacctgtac aacggtatcc ccatttacca ggaacagccg
     6781 tttctacaca gccaatcgca ctgtagtcat aggcatcttc ttccaagaca cctttctcaa
     6841 taaaggatgg aatgataatt tcatcgttgt taaaggcagg cataccaaaa ccaagtttca
     6901 tgacttcaat acattcattc atgaaacgag catctaggcc tgcatgatag cgaacagtca
     6961 agtttggctg tggcaggtgt gtttgggcaa cagatttcaa tactaagtaa gaaagtggat
     7021 tgacagcatc cttcttatca cgtgtttgtc caccaattgt tacattttgg tatagtggac
     7081 taccagcaga tgagaaggta tgtgattgac tacgaacctt gttgatagta atggtcttaa
     7141 tccacaagtt agtcaagcgt tcgacgatgc tgtcttccgt ttcacgtcct gcttctaaat
     7201 cagcattcac atacggatac atgtattggt cgaaacgtcc ataggataga gaatgtccat
     7261 tcgactcgat ttgcaagata cactggataa accagacaga ttgcactgcc tctgcaaatg
     7321 tttcagcagg ttcatacggc accttcgcac aaatccgtgc aatctccagt agttcttgac
     7381 ggcgttgagc tgttggtgct ttctctgcca attcagtagc caaggcgaca aaacgatttg
     7441 cgtatgcttt caccgcatca atgatgataa agattgagtc atagaaatga tatttatcaa
     7501 tacttgcagg atccgtcaaa tcgagattag cctttgctgc acgaactcgc tcctcaaaac
     7561 cacgcaaacc ttgttccaat accttttggt aattgacagc caagtgggca tcgccagagt
     7621 tcatcttacc ttccatacca aagaagccag tttccatata aacttgaact tcttctggaa
     7681 gcaagacacc tgcacgtgaa cgaagattat ttttttccca gaatggagcg atgctgcgca
     7741 actgctcctt ggtttcctcg gtaatataga agacgtcccc atcccgtttt tcgaataaat
     7801 ccaattcatt caatacaaac tccaaggtat attctgggaa gatgggagca tctttatttg
     7861 aagaagcttg gttacctgca atcagacttt cttcttcaat ataaagcgtc atgttctcca
     7921 atatttcctt gagcatatag gcccttttta atacattggg cttctctcta tgttcttgat
     7981 aggcttttgt tgctaaaaca gcccgttccg cgtcaatata gggcttctta tcaaagacat
     8041 cttctctgta tttgttcatc cgatctgtca gacttccaaa atactcagtt ttaatgctag
     8101 tgccagctac ttcagtcact gttttcatag ttttaccctt tctttcgaaa cttaattttg
     8161 tttcgtttgt aaattaagta tatcctgtaa taggggttat tgtcaattga tgctagtgag
     8221 cgctaagatt tccgaagccg gctgcacatt tgtgcaactc ttttgtctga catcattgac
     8281 taaactggtt ctttcgtttc actttcatag gcaatcttat cttggatttt gaagaatggg
     8341 aaataaacaa tagttgagac agctaaaata atgatttgaa caactgcacc ttgccaacca
     8401 cccaccataa aaccagaaat gattgctgga gtagaccatg gtagggtcac acctgaaaat
     8461 ggttgcataa agcctattga gattgcaccg tagacaatga aagccgctaa gatcggtacc
     8521 aaaacaaatg gtaagaacat tacaggattc atcacaattg gaaaaccaaa gacgactggt
     8581 tcatttacgt taaagagtgc aggaaaggcc gctaccttac caagagcctt atattgtttt
     8641 gatttagcgg caaatagcat tgcaaaaaca ataccgaagg taatacctga acctgacata
     8701 atcaagaaac tatctaagaa ttgttgggtc acgatgtggg cgccattttc cagagacaat
     8761 ttatctgctg ctaaaagggc cttattggca tcgaggtttg acaatagtaa agcagtaacg
     8821 acaccattga caactgactg accatgtaca ccaaaccacc agaggaagga aatgaagaaa
     8881 gcaattccaa tcgcacctgg taatgaacct gtcaaacctt gtagtggcac ttgaatcaca
     8941 tcgtaaatca tttcaatgaa tgtaccaccg tttgtcgcca tcttggaaac aatatagaca
     9001 atcatagata gcaaaaagat gacaaaagct ggaatcatag cttcaaactg cttggcaata
     9061 gcttgcggta cttgttcagg catcttgata acaatatgac gctgaataaa agtcgtatag
     9121 atagcgccca ctaccaaacc aataataatc gcaccgataa ttccttgacc accaaaccat
     9181 acttttgcga tggcatcacc gattggttct ccatcaactg gaatataaga tgatttcaag
     9241 aggataaaga atgaagacaa ggacagtacg cccgcaggga gaggctcaac tccactattt
     9301 tttgcgtaag aataaccaat cgcaaaacaa gaaatcaagc ccataatcgc aaaggttccg
     9361 ctatagactt gcataaacgg ttcagtccat tccgctccaa atatagaagc tattgcagca
     9421 tttaagcctt caaaaggcaa ctgccccaaa attaagaaaa tactaccaac tactgttaaa
     9481 ggtaagatag ccaacatacc atcttttaga gcgatgatcc ctttcatatt cacaaatttc
     9541 atgattggga caataatttt ttgtgtatca attttggaca tgaccatact cctttatgaa
     9601 accatgctga gtgccaattg aagaactttt ttcccgtcca acatcccgta gtctgccatt
     9661 ggaatcacgg caatagggac ttgcttttca tcacagagcg cttttgactt atcaagcgta
     9721 tatgctactt gtggtcccag taaagctaca tcgatttcgt caaggtaatc tgcaattttc
     9781 ccttgggaat gcgcgtcgat ttctacttcg acacctgcgt ctgctgctgc gatcttcatg
     9841 ttgtttacca acatgcctgt agaaaaacca gcggcacaaa atagagcgat tttaaccatt
     9901 tcggtttcct cctaaatata atttttttgt tttagccaac tttttttagt tctttccgca
     9961 aatcaatgat ttctttaatc aaattgatct ctgtaataga tgtcatcaaa tgatcttgcg
    10021 aatggacaaa tagtgctgta atatcagatt ttgttccccc agcttcttgt gctaagaatt
    10081 ttgtctgcat attgtgagct tctaataatg ctgcatctgc caattctaat tcctcttctg
    10141 ctcgctcttc ttcacctgct ttgacaagcg acaaagcttg gtaggcgtga cttttcgcat
    10201 ctccagcata aataatcaat cccatgatca attgatctgc aacaatcatt tccatttctt
    10261 tttcctccga tgtgttttat ttgtaagtta ttatatttct ttcgtaagta aatcatatca
    10321 ttttctcttt atagcgtaaa tatatttata gaacattttc tgttttaatt tatttctgca
    10381 cttttgtgca tttttgggga taa
//
LOCUS       DAZQCJ010000067        10328 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000067 DAZQCJ010000000
VERSION     DAZQCJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10328
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.068"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            376..927
                     /locus_tag="ACHU66_001497"
     CDS             376..927
                     /locus_tag="ACHU66_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFR3389772.1"
                     /translation="MIVFLVICIFLVRIYFLKISIKNEKRILKNGGSEFGVQNTKLLT
                     VVHILFYLSCLVEAVVRHTKFDTLSSLGLIFLLFSMGMLFWVTRLLGDIWTVKLMLVK
                     DHRFVDHWLFRVVKHPNYFLNIIPELIGLALLCHATYSFLILFPVYMVILYRRIHEEN
                     NLLKTVIIPNGTVQSAAEITGNN"
     gene            complement(1136..2044)
                     /locus_tag="ACHU66_001498"
     CDS             complement(1136..2044)
                     /locus_tag="ACHU66_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR3389773.1"
                     /translation="MKIDDLRKSSNIEDRRGQSSSNSSFSSGSSGGNVLLGPIFSRLG
                     WKGKLLVLVLLVTFGGMSNLGGLLSPSTGTNPYQSSQVTTTGTDVSDADAEFMSKVLG
                     STEDFWVQEFKANGLTYNAPTMVFYTNQTQTGCGVGSAQAGPFYCSADRKIYIDTSFY
                     QELSSKYKAAGDFAMAYVIAHEVGHHVQNELEVLGAYHQKRAQLSDKEGNALNVRLEL
                     QADYYAGAWAKYVDGQGILDAGDIDEAMNAAHAVGDDTLQQEAYGRTVPDSFTHGTSE
                     QRKKWFNLGYTYGDLAHANTFSVEKP"
     gene            2212..3555
                     /gene="xseA"
                     /locus_tag="ACHU66_001499"
     CDS             2212..3555
                     /gene="xseA"
                     /locus_tag="ACHU66_001499"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR3389774.1"
                     /translation="MVEYLSVSHLTKYLKLKFDRDPYLEKVYLTGQVSNFRKRPSHQY
                     FSLKDDKAVIQATMWAGVYKNLGFELEEGMKINVIGRIQLYEPNGSYSIVIEKAEPDG
                     IGALAIKFEQLKQALTQEGLFKEEFKQALPRFTKKIGVITSPSGAVIQDIITTVSRRF
                     PGVEIVLYPTKVQGDGAAQEVVTNIQRANERDDLDVLIVGRGGGSIEDLWAFNEEIVV
                     RAIFESRIPIISSVGHETDTTLADFVADKRAATPTAAAELATPVTKADLLGYLNQQEN
                     RAYQAMTNRLRFLRGQLEKISQSVMFRQPERLYDGYLQKLDRLTNQLQTRMKELYSQQ
                     KQACLLLQRRLQGVHPGRKVESFQERIIQQERLLKSNMANIYDNKMAKADKLIEALTM
                     LDTSRIVARGYAMIRQDGRVIDSVENVQMGENLTIQMKDGQLDVEVKHVQTDENI"
     gene            3530..3745
                     /locus_tag="ACHU66_001500"
     CDS             3530..3745
                     /locus_tag="ACHU66_001500"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR3389775.1"
                     /translation="MSKQTKTFEENLAELEGIVTKLERGDVALEEALAEFQKGMVLSK
                     DLQKTLAEAEKTLVKVMQADGSEAEMD"
     gene            3745..4614
                     /locus_tag="ACHU66_001501"
     CDS             3745..4614
                     /locus_tag="ACHU66_001501"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFR3389776.1"
                     /translation="MVASKLQRLEEAMADFYQPQVAERLNQSVLYSLQAGGKRIRPLL
                     LLTILESFGMELKQAHYQVAACVELIHTGSLIHDDLPAMDNDDYRRGRLTNHKVYDEA
                     TAILAGDSLFLDPFGFLAQVDLPAETILALVRELSQASGTFGMVAGQVLDMAGEGQNL
                     TLEQLQAIHANKTGKLLTFPFVAAGLIAGQSDVVINHLRQAGQLIGLAFQVRDDILDV
                     TTTFEEIGKTPQKDVLAEKSTYPALMGLEASKVFLEDSLNQAINELDQVAKQVPFSTE
                     KIKEIIESLRING"
     gene            4607..5428
                     /locus_tag="ACHU66_001502"
     CDS             4607..5428
                     /locus_tag="ACHU66_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HFR3389777.1"
                     /translation="MAKERVDVLAFKQGLFETREQAKRGVMAGLVISVINGERYDKPG
                     EKIDEGIELKLKGEKLKYVSRGGLKLEKALQVFHLSVEGQTTIDIGASTGGFTDVMLQ
                     AGAKQVYAVDVGTNQLAWKLRQDERVISMEQYNFRYAELGDFELGQPSFASIDVSFIS
                     LSLILPALHRILAEGGQVVALVKPQFEAGREQIGKNGIVKDKSVHLKVLEEVTKMAVE
                     TGFSVKALSFSPVQGGHGNIEFLAHLEKSTQPNQIDKEVITTTVYQAHEEFKKDE"
     gene            5421..5846
                     /locus_tag="ACHU66_001503"
     CDS             5421..5846
                     /locus_tag="ACHU66_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFR3389778.1"
                     /translation="MNKRERLEVIKDLVVRFPIDTQEEIVERLEAMGVNATQATVSRD
                     IKELGIIKVPSADKGYIYGLPKAGLVKVKSQNVFEVSVMDKMINLRLVPGSSAVVKRQ
                     ILEQFQEHVFSVIADDDSILLIVTNEGIISQIEQTVRGW"
     gene            5849..7510
                     /gene="recN"
                     /locus_tag="ACHU66_001504"
     CDS             5849..7510
                     /gene="recN"
                     /locus_tag="ACHU66_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR3389779.1"
                     /translation="MLLEVSIKNFAIIEQVSLNFENGMTILSGETGAGKSIIIDAMNL
                     MLGARATTDVIRHGAAKAEIEGLFSFENSRALEQILLEQGIEVADELIIRREILQNGR
                     SVSRVNGQMVNLSVLKQIGQYLVDIHGQHDQEELMKSQHHIRLLDSFGEDEFWSLKDR
                     YQTTFDAYRSLRKRVLEKQKNEQEHKARIEMLEYQIAEIEAADLKSGEDIQLNQERDK
                     LLNHKQIADTLTNAYALLDNEDFSSLNNLRSAMSDLQSLEEFDPDYKQLSSSLTEAYY
                     VVEDITKRLSDVVDNLDFDGNRLMQLESRLDLLNTITKKYGGTVDDVLDYFSKISEEY
                     NLLTGNDLSGDDLEVQLKNLEKELVERAGQLSQSRHELAVVLEDIIRQELQDLYMEKA
                     RFQVRFTKGKFNREGNETVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFARKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIYKIGQYGQVLAISHLPQVIAIADYQFFIEKIS
                     DEHSTVSRVRLLTKEERIEEIAKMLAGDKITDAARNQAKELVEKG"
     gene            7604..8437
                     /locus_tag="ACHU66_001505"
     CDS             7604..8437
                     /locus_tag="ACHU66_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFR3389780.1"
                     /translation="MSKIKIVTDSSTTIEPSLVEELNITVVPLSVMVDGVVYSDNDLK
                     EGEFLELMRGSKNLPKTSQPPVGLFAETFADLAKDGSQIIAILLSHALSGTVEAARQG
                     ATLSGADVTILDSSFTDQAEKFQVVEAARMAQAGASKEEILAAIETVKEKTELYIGVS
                     TLENLVKGGRIGRVQGMLSSLLNIRVIMEMKDHQLTPIVKGRGNKTFKKWLEEFTATL
                     ADKKVAEIGISYSGTADFANEMKSQLQEYVSKEISVLETGSIIQTHTGEDAWAILIRY
                     E"
     gene            8430..9263
                     /locus_tag="ACHU66_001506"
     CDS             8430..9263
                     /locus_tag="ACHU66_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFR3389781.1"
                     /translation="MNNRKLIQGFVFFFLAILGSIFLFHQLIPVASSRISRQELTVSE
                     KISFRYVALGDSLTEGVGDTTGQGGFVPLLAQSLTNDYGYEVDYKNFGVSGNTSNQIL
                     KRMKEDGELISYLKTADLLTLTVGGNDLRKAIIKNIANLKVSTFDKPAKDYGKRLDTI
                     IKTARKNNPNLPIYVVGIYNPLYLNFPELTEMQTIVDNWNTMTEQITEKYQHVYFVPI
                     NDLLYKGLEGEAGISQNGSQVTNNLLYEEDSFHPNNTGYEIMKKAILERMNETTESWE
                     P"
     gene            9235..9831
                     /locus_tag="ACHU66_001507"
     CDS             9235..9831
                     /locus_tag="ACHU66_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFR3389782.1"
                     /translation="MRPRKAGNLNIWKWLFLAQLALLIGCGIVLYTRIQTDREDLTKL
                     VQTSTEDTKVGTFSTNREQLNQTLTNYLKEYQTEEFSYQLFVTSQQVVFEGSYQIFGV
                     TIPLYIYFQPSKMEDGSILLRITEISVGSLSLPKAEVLAYLQKNYKLPAFVKIDSEQA
                     QVQVQLTELKNKFGLYGKANTIDLYNDQFIVDIYRKRQ"
     gene            9921..10196
                     /locus_tag="ACHU66_001508"
     CDS             9921..10196
                     /locus_tag="ACHU66_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFR3389783.1"
                     /translation="MANKQDLIAKVAEATSLTKKDSAAAVDAVFGAVADYLAAGEKVQ
                     LIGFGNFEVRERSARTGRNPQTGAEIKIAASKVPAFKAGKALKDAVK"
BASE COUNT         3226 a         1906 c         2309 g         2887 t
ORIGIN      
        1 atttccaact tcaaacactc cactggattg tttgaactcg ctttgctgta ctctgcaggg
       61 gtgacttgct ccgcaagtgc tatctccaac cttgcacagt cactcgactg tacaagctgt
      121 ctgcagctat tgaagcgaac ttgcttcgcc ctatctccaa cctccaaagg ttccccaaac
      181 ctttggagcg agtactaaaa gtaaactaaa agactataaa agagacatga tttcatccat
      241 ttttgaaaac aggtctcttt tattgtagtc tcaaatggaa aatagaaaaa aaacaggaga
      301 ggaatagaat ggcgacagtc taactttgta taatggatgt gtttgataaa atctgttaaa
      361 atacaggagg gatttatgat tgtttttttg gttatttgta tttttttggt gagaatctat
      421 tttttgaaga tttcaattaa aaacgaaaaa cggattttga aaaacggcgg ttcagaattt
      481 ggtgttcaaa atactaaatt attaacagtt gtacatattc tattttattt atcatgtttg
      541 gttgaagcag tagtgcgaca caccaagttt gatacgctta gtagcttagg tttgatattc
      601 ttgctgtttt caatgggaat gttattttgg gtaacacgat tgttaggtga tatttggaca
      661 gttaagctca tgttggtaaa agatcatcgt tttgttgatc attggttgtt tcgtgttgta
      721 aagcatccta actatttcct aaatattata ccagaattaa tcggtttagc tttattgtgt
      781 catgcaacat atagttttct catcttattc ccagtctaca tggttatctt atacagacgt
      841 attcacgagg aaaataattt gttgaaaaca gtgattattc caaacggaac tgtgcagtct
      901 gcagcagaga taactggaaa taattaaagt ttgctaaata ataattgtga tattgtttga
      961 aactaggata ccgctctaat tagagttgat gagtaagttt acatgattca gttttcatat
     1021 ttaaaagtgg taccgtatgt gtaaattggc ttttgtcagt tgtatattat aaagaaactc
     1081 ttgcaaaagt gtatgttgca agagtttctt tattgctacg agaggctatg gatgtttacg
     1141 gtttctctac ggagaatgta ttggcgtgag caaggtcacc ataggtataa ccgagattaa
     1201 accatttttt cctttgctca gaagtgccgt gggtaaaact gtctggaaca gtacggccgt
     1261 aggcttcctg ttgaagagta tcgtcaccaa ctgcatgggc agcattcatg gcttcgtcaa
     1321 tatcaccagc atccaagatg ccttgtccat caacatattt ggcccaggct cctgcataat
     1381 agtcagcttg caattctaaa cgaacattta gcgcattgcc ttccttatcg gatagttgag
     1441 cccgtttttg atggtaggcc ccaagaactt ctaattcatt ctgaacatgg tgaccaactt
     1501 catgagcaat gacataggcc atggcaaagt caccagcagc cttatacttg ctagataatt
     1561 cttgatagaa agaagtatca atataaatct tgcggtcagc agagcaatag aatggaccgg
     1621 cttgggcgga accaactcca catccggttt gggtctggtt agtatagaaa accatagtcg
     1681 gtgcattgta ggttaaacca ttggctttaa attcttggac ccaaaaatct tctgtcgatc
     1741 ctaaaacctt actcatgaat tcagcatctg catcagacac atctgtacca gtcgttgtga
     1801 cttgactcga ttggtaagga ttagtccctg ttgatgggga aagcaaccca ccaaggtttg
     1861 acatgcctcc gaaggttact aaaaggacaa gaacaagtag ttttcctttc caaccaagtc
     1921 gtgaaaagat tggtcccaat agaacatttc cgccagatga accagacgag aaagatgagt
     1981 tagaactgga ttgtcccctg cggtcctcaa tgttagaact ttttcgcaaa tcatcaattt
     2041 tcatataaat ctccttagaa cctgtcaaca agttcttatt ttctcacaaa ctattatata
     2101 gcaaattgtt gaaaaaaaac ataggaaagc tatcggaaat aataggccta gttctgtata
     2161 aaagtgcaaa atttggtata atagtaggga tacaatatag aaggagtgga catggttgaa
     2221 tatttatcgg ttagtcatct aactaagtat ttaaaattaa agtttgaccg cgatccgtat
     2281 ttggagaagg tgtatttgac aggtcaagtg tccaacttcc gtaaacgccc tagccatcaa
     2341 tatttttcgc tcaaggatga caaggcagtc atccaagcga ccatgtgggc aggcgtctat
     2401 aagaatctgg gctttgagct tgaagaggga atgaaaatca atgtcattgg gcgcattcag
     2461 ctctatgaac caaatggttc ttattctatt gtcattgaaa aagctgagcc agatggtatt
     2521 ggagccttgg caatcaagtt tgaacagctc aaacaagctc tgacacagga aggcttattt
     2581 aaagaagaat tcaagcaggc cttgccacgg tttaccaaga aaatcggtgt cattaccagt
     2641 ccgagtggag ctgttattca ggatattatt acaaccgtta gccgacgttt tccaggcgta
     2701 gagattgttc tctatccgac caaggtgcaa ggagatggtg ctgctcagga ggttgttact
     2761 aatattcaaa gagccaatga acgggatgac ttggatgttc tgattgttgg tcgtggcggt
     2821 ggttctatcg aagacctttg ggctttcaat gaagaaattg tggttcgggc tatttttgaa
     2881 tcccgtattc caatcatttc cagcgtaggt catgaaacag atacgacctt ggctgatttt
     2941 gtagcagata aacgagctgc taccccaaca gctgccgcag aattagcaac acctgtgacc
     3001 aaggcggact tactgggcta cctcaatcag caggaaaata gagcctatca ggccatgacc
     3061 aatcgtttga gattcctaag aggtcagctt gagaaaatta gccagtcagt catgttccgc
     3121 cagcctgaac gcttgtatga tggttatttg caaaaattag accgcttgac caatcaattg
     3181 cagacacgga tgaaagaact ctacagccag cagaaacagg cttgtctcct actacagcga
     3241 cgattacaag gtgtgcatcc aggtcgtaaa gtagaaagtt tccaagagag aattatccag
     3301 caggaacgac tcttgaagag caatatggct aatatctatg acaataagat ggccaaggcg
     3361 gacaagctga ttgaggccct gactatgctg gataccagtc gcatcgtagc ccgtggctac
     3421 gccatgattc gtcaagatgg tcgggtgatc gatagtgtag aaaatgtaca aatgggtgaa
     3481 aacctaacca tccagatgaa ggatggtcaa cttgatgtgg aggtaaaaca tgtccaaaca
     3541 gacgaaaaca tttgaagaaa acttagctga attagaagga atcgtaacca aattggagcg
     3601 aggcgatgtg gctctggaag aagcactggc cgagtttcaa aaaggaatgg ttctatcgaa
     3661 agacctacaa aagacactag cagaagctga aaagaccttg gtcaaggtca tgcaggcgga
     3721 tggcagtgaa gcggagatgg attaatggtt gccagtaaat tgcagcgact tgaggaagcc
     3781 atggcagact tttaccaacc ccaggtagct gaacgcctca accaatcggt tctttattcg
     3841 ctacaggctg gtggtaaacg aatccgcccc ttgcttttat tgaccatctt agagtccttt
     3901 gggatggaat taaaacaagc tcattaccaa gtggcagcat gtgtcgagtt gatccatacg
     3961 ggaagtttga ttcacgatga cctgcctgct atggataatg atgattaccg tcgtggtcgc
     4021 ttgaccaacc acaaggttta cgatgaagca acggctattt tagcagggga cagtctcttt
     4081 ctagatccat ttggtttcct agcccaagtt gatttaccag ctgagaccat ccttgctctc
     4141 gtgagagagc tttcccaggc atctgggacc tttggaatgg ttgctggtca agtcttggat
     4201 atggctggtg aagggcagaa tttgacctta gaacaattac aagccatcca tgccaataag
     4261 actggaaaat tattgacatt tccctttgta gcagcaggtt tgattgcagg acaatcagat
     4321 gtggtaatca accatttgcg acaagcgggt caattaattg gtctagcctt tcaagttcgg
     4381 gatgatattt tggatgtgac aaccactttt gaggaaatcg gaaaaactcc ccaaaaagat
     4441 gtattggctg agaagtctac ctaccctgcc ctaatgggac tggaagcctc aaaagtcttt
     4501 ctagaagata gcttgaatca agcaatcaac gagctagatc aagtggctaa acaagtccca
     4561 ttttcaacag aaaaaatcaa agaaatcata gaaagtttac gaatcaatgg ctaaggaaag
     4621 agtagatgta ctagctttta aacaggggct tttcgagacg cgggagcagg ccaaacgcgg
     4681 tgttatggct ggcttagtta tatccgtcat taacggggaa cgctacgata aaccaggtga
     4741 gaagattgac gagggaattg aactcaagct caagggggaa aaactcaagt atgtcagccg
     4801 tggtggtctc aaattggaga aagccttgca ggttttccac ttatcagttg agggtcaaac
     4861 aacgattgat attggggctt caacaggcgg atttacggat gttatgttgc aggctggtgc
     4921 caagcaggtc tatgctgtgg atgtagggac caaccaactc gcttggaaat tacgtcagga
     4981 cgaacgtgta atcagcatgg aacagtacaa tttccgctat gctgagttgg gagatttcga
     5041 acttggtcaa ccaagttttg cgtccattga cgtcagcttt atctcattga gtctgatttt
     5101 gccagcactc catcgtattt tagcggaggg tggccaggtt gttgcccttg tcaaacccca
     5161 attcgaggct ggacgtgaac agattgggaa aaatggcatt gttaaggata aatccgttca
     5221 cttgaaagtc ttggaagaag tgacaaaaat ggccgtggaa acaggcttct cagtcaaagc
     5281 ccttagcttt tcccctgttc aaggcggtca tggcaatatc gaatttttag cgcacttaga
     5341 aaaatcaaca caaccaaacc agattgataa agaggtcata acaactacag tctatcaagc
     5401 acatgaggaa tttaaaaagg atgaataaac gagaacgatt agaagtcatt aaagatttgg
     5461 tagtccgatt tcctattgat acacaggaag aaattgttga acgtctagaa gcaatgggtg
     5521 tcaatgcgac acaagcaacg gtttcacgtg atatcaagga acttggcatt attaaggttc
     5581 catcagcaga taagggatat atttatggtt taccaaaggc aggtcttgtt aaggtaaaat
     5641 cgcagaatgt ttttgaagtc tcagtgatgg ataaaatgat taatctccgt ctggtacctg
     5701 gaagctctgc tgtggtcaaa cgccagattt tggaacaatt tcaagaacat gttttttcag
     5761 tgattgctga tgatgacagt attctgctca ttgttacaaa cgaaggtatt atttcgcaaa
     5821 tcgaacagac ggttagaggg tggtagccat gttactagaa gtgtccatta aaaacttcgc
     5881 cattattgag caggtatcac tgaattttga aaacgggatg accatcctat ctggtgaaac
     5941 aggtgcaggt aaatccatta tcattgatgc catgaacctg atgctagggg cgcgtgcgac
     6001 gacagatgtt atcagacatg gggccgccaa ggctgagatt gaagggctct tttcctttga
     6061 aaatagcaga gctctcgagc aaattttgct tgaacagggg attgaagtag ctgacgaact
     6121 cattatccgc cgtgaaatcc tgcaaaatgg ccgttcggtc agccgtgtca atggacaaat
     6181 ggtcaattta tctgtcttga aacagattgg gcaatattta gtagatattc atggtcaaca
     6241 tgaccaggaa gaattgatga aatcccagca ccatatccgt cttttggaca gtttcggaga
     6301 agatgaattt tggagtctta aagaccgtta tcagacaacc tttgatgcct atcgtagtct
     6361 tcgcaaacga gttcttgaaa agcaaaaaaa tgagcaagaa cacaaggcgc ggattgagat
     6421 gctagaatac caaattgctg aaatcgaagc agcggatttg aagtctgggg aagatattca
     6481 actcaatcag gaacgcgata aactgctcaa ccacaaacaa attgcagata cactgaccaa
     6541 tgcctatgca ctgttagaca atgaagattt ttcaagcttg aacaacctac gctcagctat
     6601 gagtgacttg caaagtctgg aagaatttga tccagactac aaacagctct cttctagcct
     6661 gacagaagct tattatgtcg ttgaagatat aaccaagcgt ctcagcgatg tggtcgataa
     6721 tctagatttt gacggcaatc gtctcatgca attggaaagt cgtctggatt tgctcaatac
     6781 cattacgaag aaatatggtg gaactgtcga tgatgttttg gactatttta gtaaaatcag
     6841 cgaagaatac aatctattga caggcaatga tttatctgga gatgatttgg aagttcagct
     6901 taagaactta gagaaagaat tggttgaacg agcaggtcag ctcagccaat cacgccatga
     6961 attggcagtt gttttagaag atattattcg ccaagaattg caagacttgt atatggagaa
     7021 agctcgtttc caagttcgat ttactaaagg aaaatttaat cgagaaggaa atgaaacagt
     7081 agaattttac atttctacca acccaggtga agacttcaaa ccactggtta aggtagcttc
     7141 tggaggagaa ttgtctcgct tgatgttggc cattaagtca gcctttgccc gtaaagaagg
     7201 taaaacttct attgtctttg atgaggtgga tacaggggtg tctggacgtg tggcccaggc
     7261 aattgcccag aaaatttata aaatcggaca atatggtcaa gtattggcga tttctcatct
     7321 accacaagtt attgctattg cagattatca gttctttatt gagaaaatat ccgacgaaca
     7381 ttcgactgtt tctcgtgtcc gtctcttgac caaagaagag cgtattgagg aaattgccaa
     7441 gatgttagcc ggtgacaaaa tcacagatgc agcccgtaat caagcaaaag aattagtaga
     7501 aaaaggttag gcgactagcc tttttgctta tgtccaaagt ttatttttta gttaaaattt
     7561 gttataatag atggcaaaat tgaagagaat tgtaggatga aatatgtcaa agattaagat
     7621 tgttacggat tcaagtacga ctattgaacc cagtttggtc gaagaattga atataacagt
     7681 tgttccatta tctgtaatgg ttgacggagt cgtatactct gacaacgatt taaaagaagg
     7741 agaattctta gagctcatgc gtgggtcgaa aaacttgcca aaaacaagtc agcccccagt
     7801 aggactcttt gcggaaactt ttgctgattt ggctaaagat ggcagtcaga ttattgctat
     7861 tctcttgtcg catgccttgt cagggacagt agaagctgct agacaaggag caaccctatc
     7921 aggtgctgat gtgacaatac tcgattcaag ctttacagat caggctgaaa aattccaagt
     7981 tgtagaagca gcacgcatgg cacaagctgg tgccagcaaa gaagaaatcc ttgcagctat
     8041 tgaaacagtc aaagaaaaaa cggaattata catcggtgta tcaaccctag aaaatctggt
     8101 caagggtggt cgtattggac gtgttcaagg gatgctgagt agcctactca atatccgtgt
     8161 catcatggaa atgaaagacc atcagctgac tccgattgtc aaaggacgag gtaataagac
     8221 ctttaaaaaa tggctggaag agttcacggc aactctagca gataaaaaag tagccgagat
     8281 tggtatttcc tattcaggaa cggctgattt tgcaaatgaa atgaagagtc aattacaaga
     8341 gtacgtctca aaagaaattt ctgttttaga gacaggttcc atcattcaaa ctcatactgg
     8401 tgaggatgcc tgggctattt tgattcgtta tgaataacag gaagttgatt caaggctttg
     8461 ttttcttttt tctggcaatt ctagggtcga tttttctatt tcatcaactc attcctgttg
     8521 cctcatctcg catctccaga caagagctta ccgtatcaga aaaaatcagt tttcgatatg
     8581 tggcacttgg tgattctttg acagaaggtg ttggagatac aactggacag gggggctttg
     8641 ttcccttgtt ggctcaatct ctaactaacg attatggtta tgaagtagat tataaaaatt
     8701 tcggagtttc tgggaatacc agtaatcaga ttcttaagcg aatgaaggaa gatggagagc
     8761 tgataagtta cttaaaaaca gcagatctgt taactctgac agttggtggc aatgatttgc
     8821 gcaaggctat tattaaaaac attgccaatc tcaaagtttc aacttttgat aagcccgcaa
     8881 aagattacgg aaaacgattg gatacaatta ttaagacagc gcgtaagaat aatccaaatc
     8941 ttcccatcta tgttgtcggg atttacaatc cactctacct caattttcct gaattgacag
     9001 aaatgcagac aattgttgat aattggaata cgatgacaga acagataact gaaaaatatc
     9061 aacatgtcta ctttgttcca atcaatgatt tgctctataa aggattggaa ggggaggctg
     9121 gtattagtca aaatggtagc caagttacca ataatttact gtatgaggaa gacagtttcc
     9181 atcccaataa tactggatat gagattatga aaaaagcgat attggagaga atgaatgaga
     9241 ccacggaaag ctgggaacct taatatttgg aaatggctat ttttagccca gttggcttta
     9301 ttgattggtt gcggaatagt cctctataca cggattcaaa cagatagaga ggatttaaca
     9361 aagctcgttc agactagtac agaagacaca aaggttggga ccttctctac taatagggaa
     9421 cagctcaacc aaaccctcac taactatcta aaagagtatc agacggaaga gttttcctac
     9481 caattattcg taacgagtca gcaagttgtt tttgaaggca gctatcagat ttttggtgtg
     9541 actatcccac tctatattta ttttcaacct agcaaaatgg aagatggcag tattttgtta
     9601 cggataacag aaatatcagt tggaagttta tccttgccga aagcagaggt attagcttat
     9661 ttacaaaaaa attataaact acctgccttt gtaaaaatag actcagaaca agcgcaagta
     9721 caggttcaac tgacagaact gaagaacaaa tttggtctgt atggaaaggc gaatacgatt
     9781 gatttgtaca acgaccaatt cattgttgat atctacagaa aaagacagta aaatagcatt
     9841 aaaaacttga ccaaacgcct aaaattcggt actatataga agtgagtaat aactcagaaa
     9901 attattggag gacttagcaa atggctaata aacaagattt gattgcaaaa gttgcagaag
     9961 caacttcatt gactaaaaaa gactcagcag cagctgttga tgcagtattc ggagcagtag
    10021 cagactacct tgcagctggt gaaaaagtac aattgatcgg tttcggtaac tttgaagttc
    10081 gtgagcgttc agcacgtaca ggtcgcaacc ctcaaacagg tgcagaaatc aaaatcgcag
    10141 catcaaaagt tccagcattc aaagctggta aagctcttaa agacgctgtt aaataattca
    10201 gcattaaaaa gcccgtcata tcaagctttc tagcttgtcc gacgggcttt tttcggctct
    10261 ttgtcaactg tagtgggtag ataaaaagct aacacctaga gaggacgaac ttcgttctct
    10321 ctttcttt
//
LOCUS       DAZQCJ010000068        10209 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000068 DAZQCJ010000000
VERSION     DAZQCJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.069"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..1669
                     /locus_tag="ACHU66_001509"
     CDS             <1..1669
                     /locus_tag="ACHU66_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFR3389784.1"
                     /translation="ANSSQIIAGVGQLQSGASQVEQLVTGANQLQAGLEQLASSTSLS
                     AEQSSQIQALLTGLPQLQAAINQLNDSLSSIGGLAVDTSTLSSLLTEMGAQAQGLLTA
                     AQADKTAGIEALQATATYQHLPADQQAELVGALQNSPSATAAVAQTILIQLSQLSQTL
                     SSLQSLSGMETQMSQLQSAVGQINTAANQALPGATTAIENLSSGLSQVNTALNQQVLP
                     GTQVLTGGVSQLQTQLSSGASQLMSGVTAYTAGVAQLAAGGAQLVANNSSIQSGGGQL
                     TSGLATLASNSSQLVSGSGQLALGSQQLITGADQLASGGQTLTSGITSLRTGSETLTN
                     SLSSASQQLSVVSVEDKNAQAVSQPVTLEHSDQDDVKTNGVGMAPYMVSVALMVAALS
                     ANVIFVKHIDNRSYKNRWDWAKGKLLLNGIIASLAAVILYGVLRLIGIEPAHPMATLG
                     LILLASWTFMALVTALVGWNNRFGSFASLIILLLQLGSSAGTYPIELSPRFFQVIQLY
                     LPMTYSVSGLRQTVSMVGNSSHQVWMLSLFLVGFMGLGLLIYNQKDE"
     gene            complement(1721..2779)
                     /locus_tag="ACHU66_001510"
     CDS             complement(1721..2779)
                     /locus_tag="ACHU66_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFR3389785.1"
                     /translation="MKKKLTIAIIIGFVSLIAGLILAGIGFFTGGITRLEEVAAPTEV
                     HKTFTDLNTIKIDFIPHSVYIKESSDNNYHVTYANSDNNIQKPLKLSEKDGVLTLSAQ
                     ELEFAIEGIMQYLGESLAQRRINVFSVTIEVPKGKTLDKLEGHNLHFYDFGGFTIENI
                     HIKEANLSGGVTLDKVTIDSGQIEAGYFQATQSTLKDMHINLQGSSVRLYETSLENVT
                     IEGYSQLDSRQTTLLGDNRFTPSDTYSSTTFLDVTDKSLADSNLLITNQIDKKKLVEA
                     QGYYYEDGNDLGEMVNQDPYLKERLGEVGIFTSDKYTKFPVKTENDLQTLTLENKGSK
                     NKLTIEATNATINLGTPK"
     gene            complement(2776..3531)
                     /locus_tag="ACHU66_001511"
     CDS             complement(2776..3531)
                     /locus_tag="ACHU66_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFR3389786.1"
                     /translation="MTRTEYMEQLEKYLKKLPHKEYFEAISFFNEYFDEAGPERETEI
                     IEELGSPKEAASELINNMLNKQIQEEKEQQEPIQLNWKLWVGLGALSMTGLFSFFLLF
                     IMGEFIGVIPLFATLILGAFFLGRYFRNFSQTKRTLWLAILAVISLPIAIPLLLILLA
                     SLLGLVALILALIVGAFVLGVGLLTSGGYLIWEAFSLMSEGFNIFLMGFGSGLSLIGG
                     AILIYILTGFFAYWSWRLVKACFKWILKRGKRA"
     gene            complement(3518..3844)
                     /locus_tag="ACHU66_001512"
     CDS             complement(3518..3844)
                     /locus_tag="ACHU66_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR3389787.1"
                     /translation="MHFPVPAVLTEFLIMAILESDDSYGYEICQTIKLIANIKESALY
                     PILKRLEQNDFLTTYSQEYQGRMRKYYSLTQLGHEELVRLKDDWDTYTNTINGIIEGS
                     VRHDKN"
     gene            4153..4533
                     /locus_tag="ACHU66_001513"
     CDS             4153..4533
                     /locus_tag="ACHU66_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR3389788.1"
                     /translation="MKLYVQFMIILVFSFLGEAISTIFHLPIPGSIIGLILLFLALEF
                     KLIRLRHIHTVGNFLLANMTILFLPAAVGIMERFDAIKDYLLPIIIVILGAIFLNILV
                     IGFVVQFVKQKFEGDYIDAEGNHD"
     gene            4526..5221
                     /locus_tag="ACHU66_001514"
     CDS             4526..5221
                     /locus_tag="ACHU66_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFR3389789.1"
                     /translation="MTELFSNPIFGIMLSIAAYLIGMLIYRRFPHPITTPLLVATGLI
                     IVFLKMTGISYKEYYAGGSYLNMLIVPSTVALGIPLYRSFHLMKHHIRSILLGIFVAC
                     IVNTVFTALIAKWFGMDFFLAISLFPKSVTTAMAVGITDKMQGIATVTLVVVVATGIL
                     TSVLGPVFLKLLKIEDPVAVGLALGGTGHAIGTGTAIKYGHTQGAMAGLAIGITGIMY
                     VVISPIVAQIILQ"
     gene            complement(5255..6013)
                     /locus_tag="ACHU66_001515"
     CDS             complement(5255..6013)
                     /locus_tag="ACHU66_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR3389790.1"
                     /translation="MKRKRLLEFEHSFSAYAVRSILASLYLGLGIVISLYTADKLNHV
                     AEGLGKFSYGLMFGWGLLMILYMNAELGTSNMMYMTVASHRKTIPTKTALRMLATCIL
                     FNFVGAVLVCYLVSLTLPYQHVDAHSYLFEATVAKLSKTPLTQFIEGIFANIVVNIGV
                     FTFLRIKDDAGRLISVIFIIFIFAFLGYEHVIANFSLFSLAFFANGGPVEGMTLLSVL
                     SNFLFSGLGNYVGGGLFIGLLYSWLNNQSKLYVD"
     gene            6101..6946
                     /gene="gla"
                     /locus_tag="ACHU66_001516"
     CDS             6101..6946
                     /gene="gla"
                     /locus_tag="ACHU66_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaglyceroporin Gla"
                     /protein_id="HFR3389791.1"
                     /translation="MDVTWTVKYITEFLATALLIIIGNGTVANVDLKGTKGNNSGWIL
                     IAIGYGLAVMMPALMFGNVSGNHINPAFTLGLAVSGLFPWAHVAQYIVAQLLGAMFGQ
                     LVVVAVYKPYFLKTENSNHILGSFSTISALDNGTKESRKAATTNGFLNEFVGSFVLFF
                     GALALTKHFFGSELVGKLIEQGYDKTVAETMTAPYTSGSIAVAHLGIGFLVMALVAAL
                     GGPTGPGLNPARDLGPRIVHALLPKSVLGENKADSKWWYAWVGVCAPIIASIAAVALF
                     KFLYL"
     gene            7075..8607
                     /locus_tag="ACHU66_001517"
     CDS             7075..8607
                     /locus_tag="ACHU66_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HFR3389792.1"
                     /translation="MSDVIAFGYGSSRAEMQLKRLNRHGIIAGATGTGKTVTLKVLAE
                     QLSDAGIPVFLSDIKGDLNSLVAANTKEIDPSRLEKTHYLDYSPVGYPVELWDVLGEN
                     GTPIRMTISELGPVLLTRLLGLNDTQESILNIVFSVADERGLLLIDLMDLRAMLNFVA
                     ENAAELSQYYGNIPARSVGAILRSLVVLEQQGGKIFFGEPSLDIADLMRTAEDGRGVI
                     NVLQATQLFNQPTLYSTVLLSLLSELYEVLPEVGDLDKPKMVFFFDEAHVLFKDAPKV
                     LLEKIELIVRLIRSKGVGVFFVTQNPTDIPDSVAAQLGNRIQHGLRAFTPKELKTVAT
                     VAETFRQEGDEDLAKVIQELQVGEAVVSTLQADGTPSFADRVLIYPPKSMLGTVEPSA
                     LLSVINNSPLMEKYADAVNRESAHEQILAMTEAKEAELIKKAEQAEAEKQAEKEAKAA
                     AKMEEKAQKEAAKAAQKTSQPASRKTDSMMDRFTKNLMSQVGREVGRVVTRGIMGMLK
                     GK"
     gene            8719..9327
                     /locus_tag="ACHU66_001518"
     CDS             8719..9327
                     /locus_tag="ACHU66_001518"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR3389793.1"
                     /translation="MTDQLQALLKDYQPQPLGEKRSYAVFLPLVWSDDQWQVLYEIRS
                     ESISQPGEVSFPGGGIDEGETAEEAAIREVIEELDIQQKQIELLGEIDYLVFGRSTIR
                     CFVGRLHLDWQTLQPNDEVARFFTVPLETLLKTAPVYYQLDSQIVPDCDFPFERLRGG
                     VDYPFSHHKRSVPFYENLPENIWGMTAQFTHCFVEIVKSNLS"
     gene            9618..>10209
                     /locus_tag="ACHU66_001519"
     CDS             9618..>10209
                     /locus_tag="ACHU66_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HFR3389794.1"
                     /translation="MSKQKVVSSLLLSTVVLGGLAFYSTTTVKAESLEPDVTSVSSSD
                     ATNKLVVSNEEESDFLAEEELEESDAPVETLNEEIKDVTEPSSPEALLQPRAMMSDSM
                     SSGMEEIQVKDEPSDNTAEKVVKQQSDLIQSSSADSFKNGKDKEKLESSVSIDLLSSE
                     NNEIKWKVSFDTSAWSFNYHHGGLYFILPEGLNLKSV"
BASE COUNT         2886 a         2027 c         2304 g         2992 t
ORIGIN      
        1 tgccaattcc tctcagatca ttgctggggt gggacaattg caatcaggag caagtcaggt
       61 tgaacaactg gtaacagggg ccaatcaatt gcaggcaggc ttggagcaac tcgctagttc
      121 aactagtttg tctgctgaac agtctagtca gatacaggcc ttattgactg gacttcctca
      181 gttgcaggcg gccatcaatc aattaaatga tagcctatca agtatcggag gacttgcagt
      241 tgatacctct actctatcta gcctcttgac ggagatgggg gcccaggctc aaggcttgtt
      301 gacagcagcc caagctgata aaacagccgg cattgaagcc ttgcaagcaa cggctaccta
      361 tcaacacttg ccagctgatc agcaagctga attggttggc gccctccaaa attcaccttc
      421 ggcaacagcg gcagtagccc aaacgatttt gattcaattg tctcagctca gtcaaactct
      481 ttctagctta caaagtcttt ctggcatgga aacacaaatg agccagctac aatcagctgt
      541 cggtcagatc aacacggctg ccaatcaagc attgccaggt gccacaacag ccattgaaaa
      601 cctatcaagt ggcttgagtc aggtcaacac cgccttgaac cagcaagttc taccaggaac
      661 tcaggtgcta acaggtggag taagtcaact tcaaacgcaa ctgtcaagcg gagcgagtca
      721 actcatgtca ggagtaacag cctataccgc tggtgttgcc caattagcag caggtggagc
      781 ccagttggtt gcgaataata gtagtatcca atctgggggt ggccagctaa catcaggatt
      841 ggcaaccttg gcatccaact ccagtcaact tgtcagcggt tcaggacaac tggctttagg
      901 tagtcagcaa ttaattactg gtgcggatca attagctagt ggcggtcaaa ccttgacaag
      961 cggtattact agcctccgca caggtagtga aaccctaacg aattcactca gctcagccag
     1021 tcaacaacta tcagtcgtct ctgtagaaga caaaaatgca caagcagttt ctcaaccagt
     1081 caccttggaa cacagtgacc aagatgatgt gaagaccaac ggagtcggca tggctcctta
     1141 catggtgtct gttgccctaa tggttgcagc cttatcagcg aatgttattt ttgtcaaaca
     1201 cattgataat cgctcctaca agaatcgttg ggactgggcc aaaggaaaac tcttgctcaa
     1261 tgggatcatt gccagtctag cagcagtgat tctgtacggt gttcttcgct tgattggaat
     1321 cgaaccggct catccaatgg caaccttggg attaatcctc ttagcctctt ggaccttcat
     1381 ggccctcgtt acagccttgg tgggctggaa caatcgtttc ggatccttcg caagtttaat
     1441 tatcttgtta ctacaacttg gctcaagcgc aggtacctat ccaattgaac tcagcccacg
     1501 cttcttccaa gtcattcagc tctatcttcc aatgacctat tctgtttcag gtcttcgtca
     1561 aaccgtttct atggttggaa atagtagtca tcaagtttgg atgctgagtc tcttcttagt
     1621 aggctttatg ggactaggtc tcttgattta taatcaaaaa gatgaataat aagcaaacca
     1681 actgctcaat gtgtgaacag ttggtttttg gttgatgtag ttatttgggg gtccctaaat
     1741 tgattgtcgc gttggtggct tcaatggtca gtttgttctt gctacctttg ttttctaggg
     1801 ttagggtctg tagatcgttt tcagtcttga cagggaattt ggtgtactta tcgctagtga
     1861 aaataccgac ttcccctagt cgctctttta agtaagggtc ttggttaacc atctctccca
     1921 aatcattccc atcttcatag tagtaacctt gggcttctac taattttttc ttgtcgatct
     1981 ggttggtgat gagcaagttg ctatctgcca gactcttgtc cgtcacatcc aaaaatgtcg
     2041 tagaggagta ggtgtcggat ggagtgaaac gattatcgcc aagaagagtc gtttgtctgc
     2101 tatccagctg gctatagccc tcgatggtca cattttccaa actggtttca tacaggcgga
     2161 cgctgcttcc ctgtaggttg atgtgcatat cttttagagt ggattgtgtc gcttggaagt
     2221 agcctgcttc aatctgaccg ctgtcaatag tcactttgtc aagagtgaca ccaccagaca
     2281 gatttgcctc tttgatatgg atattctcaa tcgtgaaacc gccaaaatca tagaagtgga
     2341 gattgtgtcc ctctaattta tccagtgtct tccctttagg cacttcaatg gtgacggaga
     2401 agacattgat acgacgctgg gccaagcttt ctcccaaata ctgcatgatt ccctcaatgg
     2461 caaattctag ttcttgtgca gagagagtta agacgccgtc tttctcactg agttttagag
     2521 gtttttggat attgttgtcg gagttggcat aggtcacatg atagttgtta tcactagact
     2581 ctttgatgta gacgctgtgg gggataaaat caattttaat ggtattcaaa tctgtgaagg
     2641 tcttatggac ttccgtcgga gcagcaacct cttctaagcg ggttatacct ccggtgaaaa
     2701 atccaatgcc agctagaatt aagcctgcaa tcaggctgac aaagccgata ataatggcga
     2761 tggtaagttt ctttttcatg ctcgtttacc tcgtttcaag atccatttga agcaagcctt
     2821 gaccagtcgc caagaccagt aggcaaaaaa tccagtcaag atgtagatga gaatagctcc
     2881 gccaatcaaa gacaaacctg acccaaaccc catgaggaag atattgaaac cttctgacat
     2941 aaggctaaag gcttcccaaa ttagatagcc accgctggtg agaagtccga cacccaaaac
     3001 aaaggcaccg acaatgaggg ccaagatgag cgccaccaat cccaatagac tagctaggag
     3061 aatgagcaaa agtggaatgg cgataggcag ggaaatgaca gctaggatag ccaaccaaag
     3121 cgttcgtttt gtttggctaa aattacggaa gtagcgaccg aggaaaaagg cgccgaggat
     3181 aagagtcgca aacagaggaa tgactccgat aaactctccc atgataaaga gtaggaagaa
     3241 agagaagaga cctgtcatac ttaaggctcc cagaccaacc cagagtttcc agttgagttg
     3301 aataggttct tgttgttcct tctcctcctg gatttgcttg ttgagcatgt tgttaatgag
     3361 ttcgctggca gcttcttttg gtgaaccaag ttcttctata atttctgttt cacgctctgg
     3421 tccagcctcg tcaaaatact cattaaagaa gctgatggct tcaaagtatt ctttgtgggg
     3481 cagttttttg agatattttt ctaactgctc catgtattca gttcttgtca tggcggacgc
     3541 tcccttctat gatgccgttt atcgtgtttg tataggtatc ccagtcatct ttgagtcgga
     3601 ccaattcttc gtgacctaac tgagttaggc tataatactt gcgcatacga ccctgatatt
     3661 cttgggaata agtagtcaaa aagtcatttt gctccaatcg ttttaggatg ggatagaggg
     3721 cggactcctt gatgttggca attaatttaa tggtctgaca gatttcatag ccataagaat
     3781 catcggattc caaaatagcc atgatgagaa attctgtcag cactgcgggg actggaaaat
     3841 gcataggacg ctcctttcct ttgttgttag ttaggttcgg ttttgatata tatcgttcag
     3901 ttgtatatat aaaattttgt tatatattaa atatacacct acaaaaataa attgtcaagt
     3961 gattatgtgc aaaataaata aaatagctta agaccaaata gcttaggaca aatttttaag
     4021 gtgtaaacgt tttatagcct tgaaaaatat tttcagagta gtaaacttaa taacagtact
     4081 aacattaagg gaatttctgg actaaaagag aatgacctca gatggattag tagtatttaa
     4141 ggtggtgtaa agatgaaact atatgttcag tttatgatta ttctggtatt ttcattttta
     4201 ggggaagcta tttcgactat ttttcatctt cctattccgg gaagtatcat tggtttaatc
     4261 ctcctattct tagccctaga gttcaaatta attcgtcttc gccacattca tacggttgga
     4321 aattttttgc tggctaacat gaccattctt tttttacctg cagcggttgg gattatggag
     4381 agatttgatg caattaaaga ttatttactt cctattatca tcgtcatttt aggcgccatt
     4441 tttttaaata tcttagtaat tggttttgtt gttcaatttg tgaagcaaaa gtttgaaggt
     4501 gactacattg atgcggaggg aaatcatgac tgaacttttt tccaatccta tttttggtat
     4561 tatgttatca attgcagcat atttaattgg aatgctgatt tatcggagat ttcctcatcc
     4621 aatcacaacc ccactgttgg tagctacagg attgattatt gtctttttaa aaatgacagg
     4681 tatttcctat aaggaatatt atgcaggagg gtcctatcta aatatgctta ttgtgccttc
     4741 aacagtagct ctgggtattc ctttgtatcg ttctttccat ctaatgaaac atcacattcg
     4801 aagtattctt ttgggaattt ttgttgcctg tattgtcaat actgtcttta ctgccctaat
     4861 tgcaaaatgg tttgggatgg atttttttct agcaatctct ctttttccaa aatcagtcac
     4921 cactgctatg gctgttggta ttactgataa aatgcaagga atagcaactg ttactcttgt
     4981 agtcgttgtt gcaacaggaa ttctgacaag tgttcttggt ccagtctttt taaagctact
     5041 aaaaattgaa gacccggtag ctgttggtct tgctttggga ggaacaggac atgccattgg
     5101 aacgggtaca gcgataaaat atggacatac gcaaggagcg atggctggct tggctattgg
     5161 gattacaggc atcatgtatg tggtgattag tccaatagtt gcgcagatta tcttacaata
     5221 aaaaactacg attggaattc caaccgtagt ttttttagtc tacataaagt ttggattgat
     5281 tattcaacca actataaaga agtccgataa agagaccgcc acccacgtag tttcctaatc
     5341 cagaaaagag gaaattgctt agtacactta atagagtcat tccctcaact ggtccaccat
     5401 ttgcaaaaaa ggccagggag aagagtgaaa agttcgcaat gacgtgctca tatcccaaga
     5461 aagcaaagat aaagatgatg aaaataacgg aaatgagacg cccagcgtca tctttgatgc
     5521 gtaagaaggt aaatacaccg atattcacga caatattagc aaaaatacct tcaataaatt
     5581 gggtcaaagg tgtctttgaa agcttggcca ccgttgcttc aaataggtag ctgtgagcat
     5641 caacgtgctg ataaggcaga gtcagtgaga ccaagtaaca aacaaggacc gcaccgacaa
     5701 agttgaaaag gatacaggtt gccaacatcc tgagggctgt tttcgtagga atcgtttttc
     5761 gatggctggc tactgtcata tacatcatgt tggatgttcc aagttcggca ttcatatata
     5821 aaatcatgag tagcccccaa ccaaacatca acccatagct aaatttcccg agtccttctg
     5881 cgacatggtt gagtttgtct gctgtataaa gagaaatgac aatccccaat cctaaataaa
     5941 gactggctag tatcgatcgt acagcgtagg cagaaaaact gtgttcaaat tcgagtaaac
     6001 gttttctttt tatcgaatgg ctttactttg tttattatac ttgataaaat ggttgttgtc
     6061 caaaaaaaat tcaacttttg aaaggaaata taagaaaaat atggatgtta catggacagt
     6121 gaaatatatc actgaatttt tagcgactgc actccttatt atcattggta atggtacagt
     6181 tgcaaacgtt gatttaaaag gcacaaaggg aaacaactct ggttggattc tcattgcgat
     6241 tggctatggt ttggcagtta tgatgccagc attgatgttt ggtaacgttt ccggtaacca
     6301 tatcaaccca gccttcacac ttggtttggc tgtttcaggt ctcttccctt gggctcacgt
     6361 tgctcaatac attgttgccc aattgttagg tgcaatgttt ggtcagttag tggttgtagc
     6421 agtgtataaa ccttacttct tgaaaactga aaattctaac catattcttg gctcattctc
     6481 aactatcagt gcgcttgata acggaacgaa agaaagccgt aaagctgcga caactaatgg
     6541 tttcttgaat gagttcgttg gttcatttgt attgttcttc ggtgcccttg ctttgaccaa
     6601 acatttcttt ggttcagagt tggttggtaa attgattgaa cagggctatg ataaaacagt
     6661 cgctgagaca atgactgctc catatacttc aggttcaatt gcagttgctc acttaggcat
     6721 tggttttcta gttatggcac ttgttgcggc tcttggtggt ccaacaggtc ctggtcttaa
     6781 cccagcgcgt gaccttggtc cacgtatcgt ccatgccctt cttccaaaat ctgttcttgg
     6841 tgaaaacaag gctgattcaa aatggtggta tgcttgggtt ggtgtatgtg ctccaattat
     6901 cgcatcaatt gccgctgttg cacttttcaa attcttgtat ctataatata gagaacccgt
     6961 ttcgtcgggt ttttcttttt ctcttggtat atcctatata aagttgtctg ctctgctatt
     7021 ttcctaaaag ttatgctaca ataaggatag aaaaatcgga aaagaggcta gaatatgtca
     7081 gatgttattg cttttggcta tggaagcagt cgagcggaga tgcagctcaa gcggctcaat
     7141 cgtcatggaa tcatcgcagg tgcgacaggt acagggaaga ccgtgacctt aaaggtcttg
     7201 gcagagcagt tgagtgatgc gggaattccc gtctttctat cggacatcaa gggtgatttg
     7261 aatagtttgg ttgcggccaa taccaaggag attgatccta gtcgtctaga gaagactcat
     7321 tatcttgact atagcccagt cggttatcca gttgagttgt gggatgtctt gggggaaaat
     7381 gggactccta tccgcatgac tatttcagag ctgggacctg tcctactgac acgtttgttg
     7441 ggcttgaatg atactcagga gtccatcttg aatatcgttt tcagcgtggc agacgagcgt
     7501 ggccttctct tgattgattt gatggaccta cgggctatgc tcaattttgt ggctgaaaat
     7561 gcggcagagc tgagccaata ttatggaaat attccagctc gttcagttgg tgccatcttg
     7621 cgtagcttgg ttgttttgga gcaacaaggt ggtaagattt tcttcggtga gccaagcctt
     7681 gatattgctg atttgatgcg tacggcagaa gatggccgtg gggttatcaa cgttctccaa
     7741 gctactcagc tattcaatca accaactctt tattcgacag tcctgcttag cctcttatca
     7801 gaactctatg aagtcttgcc tgaggtgggc gatttggaca agcctaagat ggtcttcttc
     7861 tttgacgagg ctcatgttct cttcaaagat gctccaaaag ttcttcttga aaagattgaa
     7921 ttgattgtcc gtttgattcg ttcaaaaggt gtaggtgtct tctttgtaac gcagaatccg
     7981 acggatattc ctgatagcgt ggcagcccag ttgggcaacc gcatccaaca tggtcttcgc
     8041 gcctttacac caaaagagct taagacagtc gcgacagttg ccgaaacctt ccgtcaagaa
     8101 ggtgatgagg atttggccaa ggtcattcag gagttgcagg taggtgaagc ggttgtgtct
     8161 actctgcaag ctgacgggac accaagcttt gcggatcggg ttttgattta tccaccaaag
     8221 agtatgctgg gaacggtgga gccaagcgcc ctcttgtcag ttatcaataa ttctcctttg
     8281 atggaaaaat atgctgacgc ggtcaatcgt gagtcagccc atgagcagat tttagccatg
     8341 acagaggcta aagaagccga actcattaaa aaagcagagc aggccgaagc ggaaaaacag
     8401 gcagaaaaag aagctaaggc agccgcaaaa atggaagaga aagcccagaa agaagcagct
     8461 aaagctgcac aaaaaactag tcagccagcc agtcgcaaaa cagattccat gatggatcgc
     8521 tttaccaaga acctgatgag tcaggtcgga cgggaagttg ggcgcgtggt aactcgtggc
     8581 attatgggca tgttaaaagg gaaataaacg actagactga gaaggaaact tctcagtttt
     8641 ttatctagac aaagttcttg ccagatgcca caaaattcgt taaaataaac gtaaatcact
     8701 agttagtaag gagttaccgt gactgatcaa ttgcaggcat tattgaaaga ctatcagccc
     8761 cagccattag gtgaaaaacg ttcttatgca gttttcttgc ctctagtatg gtcagatgat
     8821 cagtggcaag ttctatatga aattcggagc gagtccattt ctcagccagg agaagtttcc
     8881 tttccaggtg gcggaattga tgaaggcgag acagcagaag aagctgccat tcgtgaggtt
     8941 atcgaagaac ttgacattca acagaaacaa attgagttgc tgggagaaat tgattatctc
     9001 gttttcggtc gctcgacgat tcgctgtttt gtcggtcgac ttcacttgga ctggcagacc
     9061 cttcaaccca atgatgaagt ggctcgattc tttacagttc cactagaaac cttgctgaaa
     9121 acggcccctg tctattatca actggattca cagattgttc cagattgtga ctttcccttt
     9181 gagcgtttga gaggtggggt tgactatcct ttcagtcatc acaaacgctc cgttcccttt
     9241 tatgaaaatc taccagaaaa tatctgggga atgaccgctc aatttaccca ttgttttgtg
     9301 gaaatagtga agagtaatct atcgtgaaat gataggttat ttttaggagg aaaaaccagc
     9361 ctttgcgata tgtatcagcg aaaaaaatgg agataaagct caatgtgagt aatgtataga
     9421 aaaacacaaa aaaaacagat gcatggcaga taaaagatag ctatggctgg tatacttggt
     9481 acggtccata tttttaagta ccatcatcta tccaggaatg cgacaatatt catgtaaacg
     9541 cattcagtgg cttgttgtca tttccatgta gatgagtggg aaaatgtata aatctatatt
     9601 ttaagaagga gccaattatg agcaagcaga aagttgtgtc cagtttatta ttaagcacag
     9661 tcgtattagg gggattagcc ttttattcaa cgacaacggt taaagcagaa tcgctagaac
     9721 cagatgttac atcagttagt tcaagcgatg ctactaataa actggtagta tcaaatgaag
     9781 aggaaagcga ctttttagcc gaagaagagc ttgaagagag cgatgctcca gtagaaactc
     9841 taaatgaaga aatcaaagat gtcacagagc catcttcacc tgaagcactt ctccaacctc
     9901 gtgcgatgat gagcgattct atgagtagcg gtatggaaga aattcaggtg aaagacgagc
     9961 cttcagataa tacggcagaa aaggtagtga agcaacagtc ggacctaatt caatcatcga
    10021 gcgcggactc ctttaagaat ggaaaggaca aagagaaact cgagtcttca gtgtctatag
    10081 acttgttgag ttcagaaaac aatgagataa aatggaaagt aagttttgac acaagtgcat
    10141 ggtcttttaa ttatcatcat ggaggtcttt attttattct tccggaaggt ttgaatttaa
    10201 agagtgtta
//
LOCUS       DAZQCJ010000069        10132 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000069 DAZQCJ010000000
VERSION     DAZQCJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10132
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.070"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            76..1356
                     /locus_tag="ACHU66_001520"
     CDS             76..1356
                     /locus_tag="ACHU66_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HFR3389795.1"
                     /translation="MTREFSFETLQLHAGQEVDSASKSRAVPIYQTTSYVFEDAQEAE
                     DLFALRKSGNIYTRITNPTVSTFENRVAALEGGIGALATSSGMAAITYTILALAHAGD
                     HIVAATTLYGGTFNLFKETLPRYGITTSFVDIDDFEAVEAAIKDNTKLVFIETLGNPV
                     INIPDLEKLAEIAHNHHIPLVSDNTFATPYLINVFSHGVDISVHSATKFIGGHGTTIG
                     GVIVDSGKFDWAASGKFPQFVEEDPSYHNISYTRDIGAAAFIVAVRVQLLRDTGAALS
                     PFNAFLLLQGLETLSLRVERHVSNAEKIVDFLLSHPQVESVNYPSLPDSPYKALADKY
                     LPKGVGSIFSFQVKGGAEEARKVIDSLEIFSNLANVADAKSLVVHPATTTHAQLAPED
                     LLAAGVTPNQIRLSVGLENINDLIEDLQLALDKL"
     gene            complement(1434..2966)
                     /locus_tag="ACHU66_001521"
     CDS             complement(1434..2966)
                     /locus_tag="ACHU66_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR3389796.1"
                     /translation="MLLATQHLTLDHQKDLRNLVHDLNFIVNPGDKIAIIGEEGNGKS
                     SLLLTLMDSTLVADYLLISGSIHRYFHSPVYIPQSLLLEMAELTLNDYFFSDLDSDID
                     YGLLYLYADQLQFDSERFSGQQLIESLSGGEKLKIQLIKSLATPSDIIFLDEPSNDLD
                     LDTLLWLENYIITSPKTILFVSHDEDFLSRTATKIIHLESVKKKTLAQTKVEELDYQD
                     YALRRQQAYQKQAQKARNDQKEFDKAMNKHLRQKSQVRNTLRNTHDATMGRLIAKKMK
                     NVLSREKRYERMKEKLTEVPFHEDAISLFFSDISPLPARKQVLHLENFQLNVEERQLV
                     SNIHFNINGQEKIGIIGQNGIGKSSFLKLIYQNLRQRADINLGYMPQRYTEVLDESKT
                     PLDFLLENGNREQERLILTHLASLQFTRQEVHHKICELSGGQKAKLLLLKMVLEQVNF
                     LLLDEPSRNFSPTSQPYIRQLFADYPGGLVCVSHDRRFLKEVCQRIYRLTENGLEELE
                     QI"
     gene            3266..3757
                     /locus_tag="ACHU66_001522"
     CDS             3266..3757
                     /locus_tag="ACHU66_001522"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR3389797.1"
                     /translation="MAITGIEQADILEIDKQVRLRRYDGQHDFAFEWHQDLELVWLID
                     GDQERYSLDLLNRMYDYLSKNGECYFIEIFEDNQFIPIGDVTLFADDFAIAIGDRRYW
                     KKGIGTKVLHRMIERARDVGLEEILVEEIYDWNLGSRKLFEKCGFEALEKTKKGWSYK
                     MIL"
     gene            complement(3813..4553)
                     /locus_tag="ACHU66_001523"
     CDS             complement(3813..4553)
                     /locus_tag="ACHU66_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR3389798.1"
                     /translation="MAQLKINVHDLHKSYGKNEVLKGITAKFYEGDVVCIIGPSGSGK
                     STFLRTMNLLETITSGQVTVDGYDLTDPKTDVDAFRSNVGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMDKSEAEKVGMELLEKVGLADKRDAMPDSLSGGQKQRVAIARALAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARKVANRVIFTDGGQF
                     LEDGTPEQIFDNPQHPRLKDFLDKVLNV"
     gene            complement(4553..6751)
                     /locus_tag="ACHU66_001524"
     CDS             complement(4553..6751)
                     /locus_tag="ACHU66_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFR3389799.1"
                     /translation="MKKKLLMLLASILPVFFIFTGVKADDTIDIVFDNAYAPFEFKDS
                     DQIYKGLDVDIINEVAKRSGWTMNQSFPGFDAAVNAVQAGSADALMAGTTITEARKKV
                     FTFSDPYFDTKIVIATTKANTISSYKDLKGKTVGVKNGTAAQNFLEENKDKYGYNVKT
                     FDTGDLMYNSLSAGAVDAVMDDEAVIQYAIQQGQDLSIDIEGEAIGSFGFSVKKGSQY
                     EYLVEDFNKALAEMKKDGTYETIMNKWLGASTTSAESTDYSSRLSLTGNASAKATPVK
                     SSYTIVADSSFAPFEYQDESGNYVGIDMELIKAIAEQQGFTITIQNPGFDAALNAVQA
                     GQADAVIAGMSITDARKEIFDFSNAYYTSNILLAVKNGSDIASYEDLKGKTVGAKNGT
                     ASYSFLEENKAKYGYTLKAFDEASSMYDSLNSGSIDALMDDEAVLLYAIQQGRNFATP
                     IPGEKSGEYGFAVKKGANPELIEMFNNGLAALVESGKYDEILNKYFNSTEEASTTTST
                     VDETTIVGLLKNNYGQLLSGLGITIGLALLSFAIAIVIGIIFGMFAVSPVKALRVTSS
                     VFVDVVRGIPLMIVAAFIFWGIPNLIESITGQQSPINDFVAGTIALSLNSGAYIAEIV
                     RGGIQAVPAGQMEASRSLGISYGTTMRKIILPQAGKIMLPNFINQFVITLKDTTIISA
                     IGLVELFQAGKIIIARNYQSFRMYAILAIIYLVVITLLTRLARKLEKGGK"
     gene            6952..7611
                     /locus_tag="ACHU66_001525"
     CDS             6952..7611
                     /locus_tag="ACHU66_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389800.1"
                     /translation="MIVRYSRWKSLLALGLSLFFGLPLSYVTVDLFISLWGEDVATVI
                     LGGILFLLIFGIPTLASHLVIIVSIQRLFFGLKAFELTEKHFVLYRGIFRRKAVSIPI
                     NEIEFICLEEQTLGAGLSRLFGNVSEKTVVSVGKTIAFATGAKTSARASLSDTYTHLL
                     ILFHDSERAVFYAKQYGIKIKNLLKYTAETSILDIEPGPIEHFSEEKLQDLLEERKLN
                     K"
     gene            7608..9593
                     /gene="uvrB"
                     /locus_tag="ACHU66_001526"
     CDS             7608..9593
                     /gene="uvrB"
                     /locus_tag="ACHU66_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR3389801.1"
                     /translation="MINRNTENQFKLVSKYSPSGDQPQAIETLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVIARVNKPTLVIAHNKTLAGQLYSEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYS
                     DSVVSLRPGQEISRDQLLNSLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREIESLTGKVLGDVDHLAIFPATHFVTNDDHMETAIAKIQAELEEQL
                     KIFEAEGKLLEAQRLKQRTDYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PEDYLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYEMEQTDTVVEQIIRPTGLLDPEVEVRPTMGQMDDLLGEINARVEKG
                     ERTFITTLTKKMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADKVTESMR
                     KAMEETARRRQIQMAYNEEHGIIPQTIKKEIRDLISVTKAVTQDKEEVVDFNALNKDE
                     RKAMIKKLEGQMQEAAEVLDFELAAQIRDMVIELKNM"
     gene            9608..>10132
                     /locus_tag="ACHU66_001527"
     CDS             9608..>10132
                     /locus_tag="ACHU66_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HFR3389802.1"
                     /translation="MKYFDTSKTTNQVVTCIRQLENTELEKELLQFIEECEKADSLSK
                     EYIERFFKIVDAFLEVFPGSSLDSQHLKKLLTLLHEKIKTEEVWAEIFYRDTSNISAY
                     SRLFRELIVRVSTYDQIRDFVDRGSIFGTLLESRESVSHTVTRLNLLILIYFVFTFLF
                     ISDTDKEKFKLQIDS"
BASE COUNT         2919 a         1901 c         2262 g         3050 t
ORIGIN      
        1 atctctaatg tttaagtatt agtcaagctg gtaaaaatga ggtagaatac agaaaaagac
       61 caaaggagta cctttatgac tagagaattt tcatttgaaa cccttcaact ccatgctgga
      121 caagaagttg attctgcttc aaaatcacgt gcagtaccca tctatcaaac cacttcctat
      181 gtttttgaag atgctcaaga agcagaagat ttatttgcct tgcgtaaatc agggaatatc
      241 tatactcgta tcactaatcc gacggtctct acttttgaaa accgtgtagc agctttagaa
      301 ggtggtattg gtgccttggc cacttcttca ggaatggcag ccatcacata cactattcta
      361 gcattagcgc atgctggtga tcacattgtg gcggcaacga cactctatgg tgggactttt
      421 aacctattca aagaaaccct tcctcgctat ggtattacaa caagctttgt agatattgat
      481 gactttgaag cggtagaagc tgcgattaaa gataatacaa aactggtatt tattgaaacc
      541 ttgggaaatc ctgttatcaa tattcctgat cttgaaaaat tggcagaaat tgcacacaac
      601 caccacatcc cgcttgtttc agataatacc ttcgcaactc catatttgat taatgtattt
      661 tctcatggtg ttgatatctc agttcattct gcaaccaagt ttatcggcgg gcatggtacg
      721 accattggcg gtgtgattgt cgatagcggc aagtttgatt gggcggcatc tggtaagttt
      781 ccgcaatttg ttgaagaaga tccaagctat cataacatta gttatactcg tgatattggt
      841 gctgcagcat ttattgtagc agttcgtgta caattgcttc gcgatacagg tgctgccctt
      901 tctccattca acgccttctt gcttttgcaa gggttagaaa ccttgtctct ccgtgtagag
      961 cgtcatgtat ctaatgctga aaaaattgtt gactttttac ttagtcatcc tcaggtagag
     1021 tcggtcaatt atccatcact tccagatagt ccttataagg ctttggcgga taagtacctg
     1081 cctaagggag ttggctctat tttctctttc caagtaaaag gcggagcaga agaagcccgt
     1141 aaggttattg atagtttgga aatcttttct aatctagcaa acgttgccga tgccaaatca
     1201 ctagtagttc atccagctac aaccacgcat gctcagttag ctccggaaga tttacttgct
     1261 gctggggtca caccaaatca aatccgccta tctgttggtc ttgaaaatat caatgatttg
     1321 attgaagatt tgcaacttgc tcttgataaa ttataggttg tgaatcgaat agcatgactt
     1381 cattctttat gaataaataa aaggaaattt ggcgtgtgtg ccattttcct tttttatatc
     1441 tgttctagtt cttccagtcc attttcagtt agtcgataga ttctttgaca aacttctttc
     1501 aaaaatcgtc tgtcgtgtga tacgcatacc aaaccacctg gataatcggc aaatagttga
     1561 cgaatatatg gctgagaagt aggcgagaag tttcgactgg gttcatctaa taagagaaaa
     1621 ttgacttgtt caagaaccat ttttaacaag agtaacttgg ctttttgtcc ccccgaaagt
     1681 tcacagatct tgtgatgaac ttcttgtcgt gtaaactgca gactggctag atgcgttaag
     1741 ataagtcgtt cctgctcacg attgccattt tctaataaaa aatcaagtgg agttttagac
     1801 tcatcaagga cttctgtata gcgctggggc atgtatccca agtttatatc tgctcgttgc
     1861 ctgaggttct gatagatgag ctttagaaaa ctggatttac caattccatt ttgtccaata
     1921 atgccaattt tctcttgtcc atttatgttg aaatggatat tgctaactaa ttgtcgttct
     1981 tcaacgttca attggaagtt ttctaagtga agtacttgct ttctagctgg tagtggtgaa
     2041 atgtctgaga agaagaggga aatggcatct tcatgaaaag gaacttctgt gagtttttcc
     2101 ttcattcttt catagcgttt ttcacgtgat aagacattct tcattttctt ggcaataagg
     2161 cgtcccatag tagcatcgtg tgtattgcgc aaggtattgc gcacctgtga tttttgtcgt
     2221 aggtgcttat tcatggcttt gtcaaattct ttttggtcat tccgagcttt ttgagcttgt
     2281 ttctgatagg cttgttggcg tctcaatgcg taatcctggt aatctagttc ttcaaccttg
     2341 gtctgtgcaa gtgttttctt cttaacagat tccaggtgaa tgatttttgt agctgttctg
     2401 ctaagaaagt cttcatcgtg agaaacaaat agaattgttt ttgggctcgt gatgatatag
     2461 ttttcaagcc aaagtaaggt atctaaatcc aagtcattgg acggctcatc cagaaaaata
     2521 atatcagatg gagttgctag agatttgatt aactgaattt ttagtttttc accacctgac
     2581 aggctttcaa tcaattgttg accggaaaat cgctcactgt caaactggag ctggtcagca
     2641 tagaggtaga gtaggccgta atcaatgtcg ctgtccaagt cagagaagaa gtagtcgttc
     2701 aaggttagct ctgccatttc cagtagaaga ctttggggaa tatagactgg tgagtgaaaa
     2761 tatcggtgaa tgctgcctga tatgagtagg tagtcggcga caagagttga atccatcaag
     2821 gttagtaata gactagattt gccatttcct tcttccccga tgatggctat tttatcgcca
     2881 gggttgacaa tgaagtttag gtcatgaacc agatttctca aatctttttg atggtcgagg
     2941 gttaggtgtt gagtggctag tagcataagg cctcctttac aagaaaaaac acactaccta
     3001 tttagtagtg tgtcgaaatc tgctcaaaaa gaccctattc aaggaataga gggaacagaa
     3061 aaatggacgc aagaaaacta ctaaataagt atagaactta aattgagttt acttgttatt
     3121 gtccatttct ccttacctcg catactttat ttattttaat atagcatatc gatagaatct
     3181 tgtcaatcag cgtgctataa tattcataga tttcgataga gatatatgtt aaaatgaact
     3241 tattgtaaat gagagaggat ccgaaatggc aatcactgga attgagcaag cagatatttt
     3301 agaaatagac aaacaagtcc gtttacgtcg gtatgatggt cagcatgatt ttgcttttga
     3361 atggcaccaa gatctagaat tggtctggct gattgatggt gatcaagaac gttatagtct
     3421 tgacttactc aatcgtatgt atgattattt gtctaaaaat ggcgaatgtt actttattga
     3481 aatttttgaa gacaatcagt ttatcccaat tggtgatgtg acgctgtttg cagatgattt
     3541 tgccattgct attggagata ggcggtattg gaaaaaggga atcgggacta aggtgttaca
     3601 tcgaatgatc gagcgtgcaa gggatgtggg tcttgaggaa atacttgtcg aagaaatata
     3661 cgattggaat cttggttcac gaaagctatt tgagaaatgt ggctttgaag ctcttgaaaa
     3721 gaccaagaaa ggttggtcct ataagatgat attgtaaatt aattttgaga taccaaaaaa
     3781 accttgacgc aagtcaaggc ttttcgttta gcttagacgt tcaagacctt atcaaggaag
     3841 tctttgagac gtgggtgttg tgggttgtca aagatttgct caggagtgcc atcttcaagg
     3901 aattgaccgc catcagtaaa gataacgcgg ttagcaacct tgcgggcgaa gcccatttcg
     3961 tgtgtgacga tcaacatggt cataccttgt tctgccaagt ccttcataac gttgagtaca
     4021 tcgccaacca tttcagggtc aagggcagaa gttggttcgt caaagagcat gatgtctggg
     4081 ttcatagcga gggcacgagc gatggcaaca cgttgcttct gaccacctga aaggctgtct
     4141 ggcatagcgt cgcgtttgtc tgccaatcca actttttcca ataattccat gccgactttt
     4201 tcagcttcag acttgtccat caatttgtgt tcgattggtg caaatgtgat attgtcaagt
     4261 acagacatat gagggaagag attgaagtgt tggaatacca ttccgacatt tgaacggaag
     4321 gcatcaacat ctgtcttagg atcggttaag tcatatccat cgactgttac ttgaccactt
     4381 gtaatggttt caagtaagtt cattgtacga aggaaggtcg atttacctga accagaagga
     4441 ccgatgatac aaacaacatc gccttcatag aacttagcag taataccttt gaggacctcg
     4501 tttttaccat aagacttgtg taagtcatgg acgttgattt ttaattgagc cattatttgc
     4561 ctcctttttc gagtttgcgt gctaagcgag tcaagagtgt gataactacc aagtaaatga
     4621 tggcaagaat tgcatacata cggaaggatt ggtagttacg tgcaataatg attttacctg
     4681 cttggaagag ttcgaccaaa ccaatcgcag aaatgatagt agtatctttc agagtaataa
     4741 caaactggtt gataaagtta ggcaacatga ttttaccagc ctgtggcaag ataatcttac
     4801 gcatggttgt tccataggaa atacccaaac tacgagatgc ttccatttga cccgcaggaa
     4861 ctgcctgaat accaccacga acaatctcag caatataggc accagagttg agggataggg
     4921 caatcgtacc agcgacaaag tcattgattg gagattgttg accagtaatc gactcgatta
     4981 agtttggaat gccccagaag atgaatgcag cgacaatcat gagcggaata ccacgaacaa
     5041 catctacaaa aacagatgaa gtaacacgga gtgctttaac tgggctgaca gcaaacatac
     5101 cgaagataat accgatgaca attgcaatag caaatgatag aagggcaagt ccgattgtga
     5161 taccaagacc tgagagcaat tgaccgtagt tgtttttcaa aagaccaaca attgttgtct
     5221 catcaacagt tgaagtcgtt gtgcttgctt cttcagttga gttgaaatac ttattaagga
     5281 tttcatcata cttgcctgat tcaactaggg cagctaagcc attgttaaac atttcaatta
     5341 attctgggtt ggcacctttt ttaacggcaa atccgtactc acctgatttt tcgccaggaa
     5401 ttggtgttgc aaagttgcga ccttgttgga tggcatatag aagaaccgct tcgtcatcca
     5461 taagagcatc aatagaacca gagtttaaac tgtcatacat acttgaagct tcgtcgaagg
     5521 cttttagggt gtaaccatat tttgccttgt tttcttctaa gaaactatat gaagcagtac
     5581 catttttagc tccaacagtt ttccctttta agtcttcgta agaagcaatg tcactaccat
     5641 ttttaacagc taataggatg tttgaagtat agtaggcgtt tgagaagtca aatatttcct
     5701 tacgagcatc tgtgatagac ataccggcaa taactgcatc agcttgtcca gcttgaacag
     5761 catttagtgc agcgtcgaaa ccagggtttt gaatggtaat agtaaatcct tgttgttcag
     5821 cgattgcctt gattaattcc atgtcaatcc cgacatagtt acctgattca tcttggtatt
     5881 caaatggagc aaaagaagaa tctgccacaa ttgtgtagct tgatttgaca ggtgttgctt
     5941 tagctgatgc gtttcctgtt aagcttaaac gagatgagta atctgtactt tcggcagaag
     6001 tggtagaagc tcctaaccat ttgttcatga ttgtttcgta ggtgccatct ttcttcatct
     6061 cagctaaagc cttgttaaag tcttcaacaa gatattcata ttggctacct ttttttacag
     6121 agaaaccaaa tgaaccgatt gcttcgcctt cgatgtcaat gcttaggtct tgaccttgtt
     6181 ggatagcgta ttgaataacc gcttcgtcgt ccatgacagc atcaacagca ccagcagaaa
     6241 ggctattata catcaaatcg ccagtatcaa aagtttttac gttgtatccg tacttgtctt
     6301 tgttttcttc taggaagttt tgggctgcag taccgttttt gacaccgaca gtcttgcctt
     6361 taaggtcctt atatgaagag atggtatttg cttttgtggt agcgataacg attttggtgt
     6421 cgaagtaagg gtctgagaaa gtaaatactt tcttacgcgc ttcggtgata gttgtacctg
     6481 ccattagggc gtccgcagat ccagcctgta cagcgtttac agctgcatca aatcctggga
     6541 agctttgatt catggtccaa cctgagcgct tggctacttc gttgatgatg tcaacatcta
     6601 atcctttata aatttgatct gagtctttaa actcgaatgg ggcgtaagca ttgtcaaata
     6661 cgatatcaat agtatcgtca gctttaacgc cagtaaaaat aaagaataca ggcaaaatgc
     6721 ttgccaataa catgagtaat tttttcttca tttccatact ccttctatta taaaataact
     6781 gtcctagtat atcatattct gaaaattaat gcaaaatcga ggtttttata atgttagaat
     6841 ttctgacatg caaagtcatt aaaatgtgag aaaatttaat gttttagggc aattgatttt
     6901 gagtaagttc aagcggactt gtggtaaaat agaatagtga aaggaatgag tatgattgtt
     6961 cgttatagca gatggaaatc tttgttggcc ttgggtctga gtttgttttt tggcttgcca
     7021 ttgtcttatg taacggttga tttatttatc agtttatggg gagaagatgt agcgacagtg
     7081 atcctcggtg ggattttatt tttactgatc tttggcattc caaccttggc ttcacatctg
     7141 gttattatcg tttctataca gcgcctcttt tttggattaa aagcatttga actgacagag
     7201 aagcactttg ttctctatcg gggaatattt cgaagaaaag ctgtgtccat tcctataaac
     7261 gaaattgagt ttatctgcct agaagaacag acactaggag caggcttgag ccgtcttttt
     7321 ggaaatgttt ctgaaaaaac agtagtatct gtagggaaga caatcgcatt tgcgactggg
     7381 gctaaaacga gtgcaagggc cagtttgtct gacacctaca cgcacttatt gatattgttt
     7441 catgacagtg agcgagctgt cttttatgct aaacaatatg gaattaagat aaagaattta
     7501 ttgaaataca cggcggaaac ttcgattctt gatattgaac ccggcccaat tgagcatttt
     7561 tccgaagaga agttgcaaga tttactagaa gaaagaaaac tgaacaaatg ataaatcgaa
     7621 atactgaaaa ccaatttaaa cttgtttcta aatactcacc gtctggtgac cagccccaag
     7681 ccattgaaac cttggttgat aacatcgagg ggggcgaaaa agctcagatt ctcatggggg
     7741 cgactggtac gggtaagacc tacaccatga gtcaggtcat tgcccgtgtc aataagccca
     7801 ctcttgtcat cgcccacaac aagactctag ctggtcagct ctacagcgag ttcaaggaat
     7861 tcttcccaga aaatgcggtc gaatacttcg tatcttacta tgattactac cagccagaag
     7921 cctatgtacc gtctagcgat acctatatcg agaaggacag ttcggtcaat gatgagattg
     7981 acaaacttcg tcattcagcg acttcagcac ttctcgaacg aaatgatgtc atcgttgtgg
     8041 cttcggtttc ctgtatctac ggtttgggat cgcccaagga atattcagat agcgtggtca
     8101 gcctgcgacc aggtcaggag atttcccgtg atcagttgct caactctctg gtagatattc
     8161 agtttgagcg caacgacatc gacttccaac gggggcgttt ccgtgtacgt ggagatgtgg
     8221 tggagatttt cccagcttcc cgtgatgaac acgcctttcg tgtggagttt tttggtgatg
     8281 aaatcgaccg cattcgtgag attgaaagcc tgacagggaa ggttttgggc gatgtggatc
     8341 acttggccat tttccctgcc acccatttcg tgaccaacga tgatcacatg gaaacggcta
     8401 ttgccaagat tcaggcggaa ctggaagagc agctcaagat ctttgaggca gaaggaaaac
     8461 tcttagaagc tcagcgattg aaacaacgga ccgactacga catcgagatg cttcgagaaa
     8521 tgggctacac caacggagtt gagaactatt cacgacatat ggacgggcga agcgagggcg
     8581 agcctcccta caccctgctg gactttttcc ctgaagatta cctgattatg attgacgaga
     8641 gtcatatgac catggggcag attaagggaa tgtacaatgg tgaccgctca cgcaaggaga
     8701 tgttggtcaa ctatggtttc cgcctcccga gtgcactgga caaccgtccg ctgcgcaggg
     8761 aagaatttga gagccatgtc caccagattg tctatgtatc tgcgacgccg ggtgactatg
     8821 aaatggagca gaccgatacc gttgtcgagc agattattcg gccgacaggg cttttggatc
     8881 cagaggtgga agtccgtcca accatgggcc aaatggacga cctcttgggc gaaatcaatg
     8941 cccgtgttga gaagggtgag cggaccttta tcactaccct gaccaagaaa atggcagagg
     9001 acttgaccga ctacctgaaa gaaatgggcg tcaaggtcaa gtatatgcac tcggacatca
     9061 agaccttgga gcgtacggag attatccgtg atcttcgctt gggtgtcttt gatgttttga
     9121 tagggattaa cctgcttcgt gaagggattg acgtgccaga agttagtcta gtcgctatct
     9181 tagacgcaga caaggagggc ttcctccgta atgaacgggg gctcatccag acaatcggtc
     9241 gagcggctcg taactctgaa ggtcatgtga ttatgtatgc ggacaaggtc accgagtcca
     9301 tgcggaaggc catggaagaa acagcccgcc gccgtcaaat ccagatggct tataatgaag
     9361 aacatggcat cattccacaa actattaaga aagaaatccg tgacctgata agcgtgacta
     9421 aggctgtcac tcaggacaag gaagaagtag tggacttcaa tgctcttaat aaagatgaac
     9481 gcaaggctat gatcaagaaa ctggaaggtc agatgcagga agcagcagaa gtgcttgact
     9541 ttgaactggc agctcaaatt agggatatgg tcattgagtt gaagaatatg tagatatgga
     9601 ggtagttatg aaatattttg atacgtcgaa aacaactaat caagtggtaa cctgtattag
     9661 acaattggaa aatacagagc tagaaaaaga gttgttacag tttattgaag agtgtgagaa
     9721 agcagactct ctatccaaag agtatataga acgttttttt aaaattgtcg atgcttttct
     9781 tgaggtgttt ccaggatcat ccttggattc gcagcactta aagaaactct tgactttgct
     9841 ccatgaaaaa ataaaaacag aagaggtgtg ggcggaaatc ttttatagag atacctctaa
     9901 cattagtgct tattctagat tatttaggga attaattgtt cgagttagca cttatgatca
     9961 aattagagat tttgttgata ggggaagtat ttttgggacg ctattggaaa gtagagaaag
    10021 tgtgagtcat actgtaacca gactcaattt actaatactg atatatttcg tatttacctt
    10081 tttgtttatt tctgatacgg ataaagaaaa gtttaaatta cagatagatt cc
//
LOCUS       DAZQCJ010000070        10029 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000070 DAZQCJ010000000
VERSION     DAZQCJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.071"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            56..1846
                     /gene="dnaG"
                     /locus_tag="ACHU66_001528"
     CDS             56..1846
                     /gene="dnaG"
                     /locus_tag="ACHU66_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR3389803.1"
                     /translation="MLSKDKITEIKQALNIVDVIGETVALTKAGRNYVGLCPFHGEKT
                     PSFNVIEDKQFYHCFGCGKSGDVFKFVEEVRGVSFADAAAILAEKAGFQTDVTPSYHQ
                     EEKRVSPHQVLYDIHRDAAKFYHALLMTTKMGEEARAYLHQRGLTDDVIKTFQLGLAP
                     AEQNILYQKLSGQYDEESLLNSGLFNPSENNIIYDAFQARIIFPLADEYGRIVAFSGR
                     IWTEENRNNKQLAKYKNSRSTAIFNKSYELYHLDKAKAVIKKQRETYLMEGFLDVIAA
                     HRVGIDNAVASMGTALTREHVAHLAKFCKKIVLTYDGDKAGQAATMKALDELQDFQVE
                     IVSLPDNMDPDEFLQKNSEEALQQVLTKSRISDVEFLIQYLKPENPDNLQMQIEFVDK
                     IAPIIAKVSSITAQNSYIYKVADLLSDFDYNQVEQAVNAVRLNQRQERTQQVFQSQTG
                     QSYLPPVQTIARITSLIRTENHLLYRMVEHPYILNEFRLREDFYFATPELQAIYDMLK
                     KSGEITSFELSQLNDSAQQAWYRMQEERLPDEVSPHEIEELEIRRDKELLKKENQHLA
                     RKIREHSHVGNADIALDELQKLLDRRRQME"
     gene            1849..2967
                     /gene="rpoD"
                     /locus_tag="ACHU66_001529"
     CDS             1849..2967
                     /gene="rpoD"
                     /locus_tag="ACHU66_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR3389804.1"
                     /translation="MTNKKDKKTEVTTFDVQVAEFIRNHKKQGSATDDEINDQLVIPF
                     TLDADGIDDLLQRIQDAGISIVDKEGNPSARAMQVEEEPELSDEELLGSTSAKVNDPV
                     RMYLKEIGVVPLLTNEEEQELALAVEAGDPEAKQRLAEANLRLVVSIAKRYVGRGMQF
                     LDLIQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVET
                     INKLVREQRNLLQELGQDPTPEQIAERMDMTPEKVREILKIAQEPVSLETPIGEEDDS
                     HLGDFIEDEIIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLED
                     VGKVFNVTRERIRQIEAKALRKLRHPSRSKPLRDFIED"
     gene            3352..3675
                     /locus_tag="ACHU66_001530"
     CDS             3352..3675
                     /locus_tag="ACHU66_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFR3389805.1"
                     /translation="MTYTEDQIKDIQERIFHALEDVIDPELGIDIINLGLIYEIRFIE
                     GKAEIDMTLTTMGCPLADLITDQIHDVLKDVPEVTEVDVRLVWSPAWTVQKMSRYARI
                     ALGIK"
     gene            3856..4013
                     /locus_tag="ACHU66_001531"
                     /pseudo
     CDS             3856..4013
                     /locus_tag="ACHU66_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775157.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4100..5230)
                     /locus_tag="ACHU66_001532"
                     /pseudo
     CDS             complement(4100..5230)
                     /locus_tag="ACHU66_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            5326..5649
                     /gene="tnpA"
                     /locus_tag="ACHU66_001533"
                     /pseudo
     CDS             5326..5649
                     /gene="tnpA"
                     /locus_tag="ACHU66_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373723.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5789..6382)
                     /locus_tag="ACHU66_001534"
     CDS             complement(5789..6382)
                     /locus_tag="ACHU66_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR3389806.1"
                     /translation="MYKFWQKTIQVLSILTLIGTAIFLFWLYKIGILNDQNVLSDLIK
                     SQGALGSLSFLAIQIIQVVFPIIPGGVTTVVGFLVFHFWWGFFLNYLGISIGSIILFW
                     LARRYGKKFCLLFMSEDTFYKYESKIDNKRSYEIFFILCMLSPISPADIVVMITGLTS
                     MSYRKFIMITLLCRPFSVVAYSFFWIYGSQWLQQLLG"
     gene            6492..6839
                     /gene="ssrA"
                     /locus_tag="ACHU66_001535"
     tmRNA           6492..6839
                     /gene="ssrA"
                     /locus_tag="ACHU66_001535"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            6842..6929
                     /locus_tag="ACHU66_001536"
     tRNA            6842..6929
                     /locus_tag="ACHU66_001536"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6876..6878,aa:Ser,seq:gga)
     gene            7088..8089
                     /gene="add"
                     /locus_tag="ACHU66_001537"
     CDS             7088..8089
                     /gene="add"
                     /locus_tag="ACHU66_001537"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR3389807.1"
                     /translation="MLNVNELVKTELHCHLDGSLSLGVIRQLAQMAKITIPAEDEALR
                     KLVSVHGKVDSLMAYLKLFDFVRPLLQTATALELAAYDLVRQAASDKVIYIEVRFAPE
                     LSTDQDLTILEAVSAVLVGLNRGQEDFGVLAKLLVCGLKQTNTNQTKELFSAIADLAP
                     KGLVGFDFAGNEADYPTEELRDIIQFTQSLGYPMTFHAGECGCVINVIQALELGIRRI
                     GHGTALTRNPEAIQAFVNSGATLEMCLTSNLQTGAADSIEYFPYRELVEAGANITINT
                     DNRTVSNTTLNREYQLFVEYFGTSKADFYRFNQNAIQASFASEGEKKVLLELLDQQY"
     gene            complement(8133..8915)
                     /locus_tag="ACHU66_001538"
     CDS             complement(8133..8915)
                     /locus_tag="ACHU66_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR3389808.1"
                     /translation="MFKKLLKYEFQSVGKWYLGIYIAAVVLSAVLGFWLQALALRTQA
                     GNTEPGGGEMVLLGTSFMTFGILIAALFLSTFVLVINRFRKNVYGRQGYLTMTLPVSS
                     YQIILSKLLASIVWYILAGITCLLSIGIILAVILAASEEAIMPEIQTFVQAIDWTVLF
                     SQIFYYLIEITTGILLIYFSISVGQLFKDHRFLFAILTYIGILIVTGVFSTFFVLSNL
                     GTAYSTPFSLYPSPVLALVNIILAFAYYFGTHFIMTKKLNLQ"
     gene            complement(8932..9639)
                     /locus_tag="ACHU66_001539"
     CDS             complement(8932..9639)
                     /locus_tag="ACHU66_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3389809.1"
                     /translation="MSNAYTLVELQQVSKSYGGAVALNNVNLKLTAGKIIGLLGPNGS
                     GKTTMIKLINGLLQPEYGQVLINGRTPSPETKAIVSYLPDTTYLDEHMKVLEAITFFE
                     DFYADFDKERALHLLQDLGIDLNSRMKHLSKGNKEKVQLILVMSRQAQLYVLDEPIGG
                     VDPAARDYILKTIVNNYSPTASVIISTHLISDVEQILDEVIFLHYGSVIRHGNVDDLR
                     IESGESIDELFRQDFKA"
     gene            complement(9632..10006)
                     /locus_tag="ACHU66_001540"
     CDS             complement(9632..10006)
                     /locus_tag="ACHU66_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR3389810.1"
                     /translation="MAWKFDNNIPIYIQISNTIKLQIVTNQLKSGDKLPTVRDLADIA
                     GVNPNTVQRALSDLETEGFVYSVRTTGRFVTDNQELITQTRLHLAQKELENFVNNMLE
                     LGFRHDELTSQLDDYLKGVTYE"
BASE COUNT         3155 a         1888 c         2087 g         2899 t
ORIGIN      
        1 actaagggga gagtgttcac tttttaaatt agctagtatg atatgaggag ggaatgtgct
       61 ttcaaaggat aaaattactg aaataaaaca agctctcaat atcgttgatg ttattggtga
      121 aacggttgct ttgactaagg ccggacgtaa ttatgtcggt ctttgccctt ttcatgggga
      181 gaaaacccca tctttcaatg tgattgaaga taagcagttc taccattgtt ttggatgtgg
      241 taagtccgga gatgtattca agtttgtcga agaagttcgt ggtgtttctt ttgcagatgc
      301 ggcagctatc ttagctgaga aggcagggtt tcaaacagat gtaactcctt cctatcatca
      361 agaggagaaa agggttagtc cgcatcaggt actgtatgat attcatcggg atgctgcaaa
      421 gttttatcat gcccttttga tgacaaccaa aatgggtgag gaagctagag cttatctaca
      481 ccaacgtggc ttgacagatg atgtcataaa aacattccag ctaggcttag ctcctgccga
      541 acagaatatt ctttaccaaa aactatcagg tcaatatgac gaagaaagtc tattgaattc
      601 gggtttgttc aatccaagtg agaataatat tatttatgac gcctttcaag cgaggattat
      661 tttccccttg gcggatgaat atggtcgaat tgtagctttt tcaggtcgga tttggaccga
      721 agaaaatcga aacaataaac aattagctaa gtataaaaac tctcgtagta ctgcgatttt
      781 caataaaagc tatgaattgt atcatttgga caaggctaaa gcggttatta agaagcaacg
      841 tgaaacctat ctcatggaag gttttctaga tgttattgct gctcatcgtg tagggattga
      901 taatgcagtg gcttccatgg gaacagcttt gacaagagag catgttgcac acctagctaa
      961 gttttgtaag aaaattgttc tgacttatga tggggataag gctggacagg ctgctaccat
     1021 gaaagcgttg gacgaactac aagattttca ggttgaaatc gtgagcttgc cagataatat
     1081 ggacccagat gaatttttgc agaaaaattc ggaagaagcc ttgcaacagg ttttaaccaa
     1141 gtcacgaatc agcgatgtag aatttttgat tcaatacttg aaacctgaaa atcctgacaa
     1201 tctacaaatg cagattgagt ttgtagataa gattgcacca attattgcca aagtatcatc
     1261 cattacagct caaaattctt atatttataa ggtggcggat ttactttctg actttgatta
     1321 taatcaggtg gaacaggcag taaatgctgt tcgtctgaac cagcgccagg agagaacaca
     1381 acaggtattt caatcacaga ctggtcagtc ttatttgcca ccagtccaaa cgattgctcg
     1441 tataacgagt cttattcgaa cagaaaacca tttactttat cggatggttg aacatcctta
     1501 tattctcaat gagttccgtt tacgagaaga tttttatttt gcgacgccag aattgcaagc
     1561 tatttatgat atgttaaaaa aatctgggga aattaccagt tttgaactat cacagttgaa
     1621 cgattccgct caacaggctt ggtatcgtat gcaggaagaa cgtttacctg atgaagtgtc
     1681 tccacacgaa atcgaagaat tagaaattcg gcgagacaag gaattgttaa agaaggagaa
     1741 ccagcaccta gcacggaaaa tccgtgaaca ttcccatgtg ggaaatgcag atattgccct
     1801 cgatgagttg caaaaactcc tcgatagaag aagacaaatg gagtaaatat gacaaataaa
     1861 aaagataaaa aaacagaagt aactactttt gatgtacaag ttgccgaatt tatccgtaac
     1921 cataaaaaac aaggttcagc tacagacgat gaaatcaatg accaactggt tattccattt
     1981 acgcttgatg ctgacggaat cgatgatttg ctccaacgta ttcaggacgc agggatttcg
     2041 attgtcgata aagaagggaa cccttctgct cgtgctatgc aggtagagga agaaccagaa
     2101 ttatctgatg aagaattgct cggaagcact tctgcaaaag tgaacgatcc agtccgcatg
     2161 tatttgaaag aaattggtgt tgtacctctt ttgaccaacg aggaagagca agaattggca
     2221 ttggcggtcg aagcaggtga cccagaagcg aaacaacgtt tggctgaagc caacttgcgt
     2281 ttggttgtat ccattgccaa acgctatgtc ggtcgtggta tgcagttcct tgacttgatt
     2341 caagaaggaa acatgggctt gatgaaggcc gttgataagt ttgactactc taaaggtttc
     2401 aaattttcaa cttatgcaac ctggtggatt cgtcaggcta tcactcgtgc cattgcggac
     2461 caagcgcgta caatccgtat ccctgttcac atggtagaaa ccattaacaa attggtccgt
     2521 gaacaacgca atctcttgca ggaattgggc caagatccaa ctcccgagca aatcgctgag
     2581 cgtatggata tgacgcctga gaaagttcgt gaaatcctca agattgcaca agagcctgtc
     2641 tcgctcgaga ccccaattgg tgaagaagat gatagtcatc ttggagattt tatcgaagac
     2701 gagatcattg aaaatccagt tgattacaca actcgtgtcg ttcttcgtga acagttagat
     2761 gaggtattgg atactcttac tgaccgtgag gaaaacgtat tgcgtcttcg ttttggttta
     2821 gatgatggta aaatgcgcac gcttgaagat gttggcaaag tctttaacgt aacccgtgaa
     2881 cgcatccgcc aaatcgaagc caaagccctc cgtaaactcc gccacccatc ccgcagcaaa
     2941 ccactcagag attttattga ggattagaaa ggtactgcgt acctttctaa cgcctgccca
     3001 gaaataagaa agcaggctaa cccagtggtg ttttaacccg agcctagaaa taggaaagcg
     3061 aggctaatct ccgggggagg tgatcgacgt ttctcatatc attcgaccat aatgatgtga
     3121 gaaactagta agtcgtctag gcaattctat tgggaattgc cgttagttga gtagcttttc
     3181 aaagcactca actttggcga gttgaaccgc tcgctgaaaa caggaaacga gcgagatttt
     3241 aaccgctccc cttgaaatta gaaagggagc gtaacgtcca gtggactttt tgacacaaac
     3301 ttcgtttgtt tcttttctag tattgaactt gtgaaaaaga aagagagaaa aatgacgtac
     3361 acagaagatc aaattaaaga cattcaggag cgtatttttc atgctcttga agatgtgatt
     3421 gaccctgagc taggtattga tattatcaat ttagggctaa tttatgaaat ccgatttatt
     3481 gagggcaagg ctgaaattga tatgaccttg acgaccatgg gttgtccatt agctgatcta
     3541 attaccgatc aaattcatga tgtattgaag gatgtccctg aagtaacaga agtagatgtg
     3601 agactggttt ggtcaccagc ttggaccgtt caaaaaatga gtcgttatgc tcgaattgca
     3661 ttaggaatta aataaaaaaa caaaatggtt tcgaacgttt gaagccgttt ttttattatg
     3721 gcttcgagcc agtttttctc gtagagaaaa acaagaaagc caccagctat gctggtggct
     3781 gccaaggctt tgagtttcca tttctttttc ctgttataat ctaattgttc aggttagatc
     3841 aaggaggaaa aagaaatggc taaaaccagt tacagtttat cacatactaa atggatgtgc
     3901 tctatcacat agttttcaca cctaagtacc gtagaaaatc catttactac aaaataaggc
     3961 aggacttaat tgatattttc cgtcatctat gtcgatgcaa aggcgtggac atcacgtcat
     4021 acgcatgaaa aatttcttct ctccttttgt ccccggaaat aactgcttta aacttagtat
     4081 ctaccttttt acaagcacct tatccccgat ttccaaataa ttgtggcaaa taatcctcca
     4141 aatggacaga aatatagcgt tgtttgcggc ggtaactcaa tttctcctta gggaaggtca
     4201 tcatttgtaa gagatgaaga caagccttac gaatagcatt taaattgaaa gcagctcgtt
     4261 tatctaaggt ttggttctta tcttctcggt aaaccacatc taacaaccaa tgtaggctct
     4321 caaccgacca atgaccacga acaacctgag aaaaagtttg cacatcgccc ttgaaactaa
     4381 ggataaagta acggacctct tcagtgatga caccatcttt atcaatgata ttcttagtca
     4441 taccaatccc acgaagtttt ttccatttcg gatgtcgttg gctcagccat ttcacatcgt
     4501 gagaaaccca gtagtccctc acctcaatct gactgcgtgc cttttcaatg gtctgataat
     4561 gacagccttt ctctgtcaac gtagataaca atttggcgtc actaaaatag aggtcaatat
     4621 cctcatggag attgccttga tttcctttga ctgccaagca ataatcaccc ttaccgctga
     4681 taatcacatc cgcaatgtct gtctaggtgc ccatggcatc aatcgtgaca acactcttac
     4741 gaaggtctag ctgacacaag aggcgaggaa tggctgtgat ttcattactt ttatcctcaa
     4801 cagctacctg cccaaggcta atccgattac caccatcata agcagtgaca atatgagtag
     4861 gagattggtg tttccctcgg ttcccccgaa tcgtttttcc gtcaacggca atcaattttg
     4921 aaaaatcagt tgtttccgaa ctagcttcga aggacagttt cagttccttt aagaaatctg
     4981 gattgaccat actcacaaca cgttccaagg tgtcatgaga aggacaacca gcactcaagt
     5041 caacgtattc tctcaagaca gattcgttca tttctatgaa atcttccatc tctttccacg
     5101 tttcaacccc tgctaattga caagcgaaaa ccagaaacag aatggtagat agaggatatc
     5161 ggattttcca tgcttgacgg ctatcatact cttctttgtg ctcttctatg gataaaagaa
     5221 aatcaatcaa agtagttcac ctctattcta gtgtactact ttgattgata tttggttatg
     5281 atttccgctt tctgaaaacg attttttctt catgcgtttg gcgtggtgga aatcattgag
     5341 ggacacatga tgccagacca tgttcatatg cttgtcttga tatccccaaa actttcgatt
     5401 tccgatttta tgggatattt gaaaagtaaa agtgctctca tgatatttga taaacatgct
     5461 aatttaaaat ataagtatgg aaatcgaaag ttttgggcta gaggctacta tgtgagtatc
     5521 gttggactga acgaaaagac ggtagcgaaa tgtattcgcg agcaggagaa aaatgacatc
     5581 gcacttgata agttaagtgt taaagaatat gaagattcat tttcagatag tagttttaga
     5641 acaagataaa agcccgttgt tacgggcttt agtcaagtaa ttatagcact aacctgaacg
     5701 aagttcagcg aacgtcttta gacgtcgctg gagagaaacg gcttatagcc ggtgtacaag
     5761 ccacccgttt tcacgggtgg ttatgatttt atcctaacaa ttgctgcaac cattggctgc
     5821 catagatcca aaagaagcta taggccacaa ctgagaaagg gcgacaaagt aaagtaatca
     5881 taataaattt ccgatagctc atgcttgtta atccagtaat catcaccact atgtcagctg
     5941 gagaaatggg agaaagcatg caaagaataa agaaaatttc ataactacgt ttattatcaa
     6001 ttttgctttc atatttgtaa aacgtatctt cagacatgaa gaggagacaa aatttctttc
     6061 catagcgacg agccaaccag aaaaggatga tactaccgat tgaaattcca agataattga
     6121 gaaagaatcc ccaccaaaaa tgaaatacga ggaaacccac aacagtcgta acaccgcctg
     6181 gaataatcgg aaatacaact tgaataatct gaatagctaa aaaggagagg ctacctaaag
     6241 caccttggct tttgattaaa tcgcttaaga cattttggtc gttcaaaatg ccaatcttat
     6301 agagccaaaa cagaaaaata gcagtcccga taagggtcaa aatacttaat acttgaatgg
     6361 ttttttgcca aaatttatac atactttcat tttaccatat ttcaaaattt ttttaaattg
     6421 taaggaaaat gtaaaataat tttgctattt ataaaaaatt ctgctataat aattcctgta
     6481 tcaaatccta cggggttgtt acggattcga caggcattat gaggcttgtt ctgcaactca
     6541 tcggcagatg taaaaatgcc agttaaatat aactgcaaaa aatacaaaca cttacgcatt
     6601 agcagcttaa taacctgcct gcgtgaccag tcgcgaattg cttgtgtttg ctgattggtc
     6661 ttaaaatagc aagctacgtt gggattgagt caggcagttc caaaagagat ttactgactc
     6721 gcttggtcta ggtttgagtt atgtatcgag ttcaagttaa agcaagacat aacctatggt
     6781 tgtagacgaa taagttagca ggtgtttgga cgtgggttcg actcccacca gctccatata
     6841 tggaagatta ctcaagaggc tcaagaggcc gtgttggaaa cgcggtaggc gtgtaacagc
     6901 gtgcgtgggt tcgaatccca tgtcttccgt atgttattga agaacgactg atagtcgttc
     6961 tttttttgtc tgtgaatgat acaactgaaa aacaagtgaa agttcaggtt ttatggtaaa
     7021 attcttgttt ttcttcaaaa atatgatatg atagttaaca atataccaga cggaggttgt
     7081 gttaatagtg ttgaatgtaa atgaattggt caaaacagag ttgcattgtc atttggatgg
     7141 ctcattgtcc ctcggagtca ttcgacaatt ggcacagatg gctaagatta ctattcctgc
     7201 agaagatgaa gcattaagaa aattagttag tgttcatgga aaagttgata gtttaatggc
     7261 ttatttaaag ttatttgatt ttgttcgccc actcttacaa actgcaacag ctttagaatt
     7321 ggcagcctat gatcttgtac gacaagctgc aagtgataag gtcatttaca ttgaggttcg
     7381 ttttgcacct gaactgtcga cggatcaaga cttgactatt ttagaagcag tctcagcagt
     7441 tttagttgga ctaaacaggg gacaggaaga ttttggagtg cttgctaaac ttctggtttg
     7501 tggtttaaag caaacgaata cgaatcaaac gaaggaactc ttttcggcta ttgctgattt
     7561 ggcaccaaaa ggacttgtgg gatttgattt cgcaggaaat gaagcagact atcctacgga
     7621 agaacttcgg gatattattc aatttactca gtctttaggt tatcccatga ctttccatgc
     7681 cggagaatgt ggatgtgtaa tcaatgtgat tcaagctctt gaacttggga ttagaaggat
     7741 tggtcatggc acagcactta ctcgcaatcc tgaagctatc caagcttttg tcaatagcgg
     7801 agcaacactt gaaatgtgtc taacatcgaa tcttcaaact ggtgcagctg attcgattga
     7861 atattttcca tatcgtgagc tagttgaggc aggagcaaac atcacaatca acacagataa
     7921 tcgtaccgtt tctaatacta cacttaatag agaatatcag ttatttgtag aatattttgg
     7981 gactagcaag gctgattttt atcgcttcaa tcaaaatgct attcaggcaa gttttgccag
     8041 cgaaggagaa aagaaagttt tgttggaact cctagaccaa cagtattaag aaaaaagctc
     8101 tataccggtc gattagacca gtaaagagct ttttattgta gatttaattt ctttgtcata
     8161 atgaagtggg ttccaaagta gtaagcaaaa gctaggataa tattgactaa tgctaagact
     8221 ggacttggat agagagaaaa tggtgtagaa taagctgttc ctaggttact taggacaaag
     8281 aaagtgctaa atactccagt aacgatgaga atgccgatat aagtcaagat tgcaaagagg
     8341 aagcgatgat ccttgaataa ttgaccgaca gatatagaga agtagatcaa aaggatacca
     8401 gtggtaattt caattaaata atagaaaatc tgactgaata gaactgtcca gtcgatagct
     8461 tgaacaaaag tttgtatttc tggcataata gcttcttcgc ttgctgctag aatgactgca
     8521 aggatgattc caattgaaag taagcaagta atacctgcca agatatacca aacaatagag
     8581 gcaagtagtt tgcttaagat gatctgatag ctagataccg gcagggtcat agtgagataa
     8641 ccttgtcggc cataaacatt ttttctaaaa cggttgatta ctagaacaaa tgttgacaag
     8701 aagagtgcgg caatcaaaat accaaaagtc ataaaggatg ttcccaataa gaccatctct
     8761 ccaccgccag gttcagtatt tccagcctgt gtacggaggg ccaacgcttg tagccaaaaa
     8821 ccaagtactg ctgataggac cacagctgca atataaattc ccaaatacca tttacctacg
     8881 gattgaaatt cgtatttcaa taatttttta aacatagcaa tctccttcta actaagcctt
     8941 aaaatcttgg cggaagagtt cgtcaataga ttctccgctt tcaattcgca agtcatctac
     9001 attcccatgg cgaataacac ttccgtagtg taggaaaata acctcgtcca aaatttgctc
     9061 aacatctgaa attaagtgag tagagataat aacagaagca gtcggagagt agttgttgac
     9121 gatggttttt aaaatgtagt ctcgggcggc agggtcaaca ccaccaattg gctcatctaa
     9181 aacatacagc tgtgcttgac ggctcatcac cagaatgagc tggacttttt ccttattccc
     9241 cttagagaga tgtttcatac gactgtttaa atcaatgccc aaatcttgta aaagatggag
     9301 agcacgctcc ttatcaaaat cagcgtaaaa atcttcaaaa aatgtaattg cttcgagcac
     9361 tttcatatgt tcatcaagat aagtggtatc tgggagataa gatacaatcg ccttggtttc
     9421 tggcgatggt gtgcgaccat tgatcaatac ttgaccatat tctggctgca acagaccgtt
     9481 gatcaattta atcatggttg ttttacccga accatttggt cccaaaagtc ctatgatttt
     9541 tccagcagtc aacttcaaat tgacattatt tagggcgaca gctccaccat aagattttga
     9601 aacttgctgt agctcaacca aagtatatgc gttactcata agtaactcct ttcaaataat
     9661 catctaactg gctagtgagt tcatcgtgtc taaagcccaa ttctaacata ttgttcacga
     9721 agttctccag ttccttttgg gctaaatgta ggcgagtttg tgtaatcaat tcctgattat
     9781 ccgttacaaa acggcctgtt gtgcgtactg agtaaacaaa tccttctgtt tctaaatcag
     9841 acaaagcgcg ctgaacggta tttggattga caccagcaat gtcggctaag tcacgaacag
     9901 tgggaagttt atctccagat tttaattgat tggtcacaat ctgcaattta atagtattgc
     9961 tgatttgaat atagatgggg atgttattat caaatttcca agccataatc gtttcctttc
    10021 taggatgac
//
LOCUS       DAZQCJ010000071         9927 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000071 DAZQCJ010000000
VERSION     DAZQCJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9927
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.072"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..266
                     /locus_tag="ACHU66_001541"
     CDS             <1..266
                     /locus_tag="ACHU66_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HFR3389811.1"
                     /translation="TLKAISAPIRRQILDSLKSGPKSAGEIVEQFQLTGATVSHHLST
                     LKKAGLILEEKQKNFIYYRLNYTVFEEVLVWIASFGGQQDEEN"
     gene            253..855
                     /locus_tag="ACHU66_001542"
     CDS             253..855
                     /locus_tag="ACHU66_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HFR3389812.1"
                     /translation="MKKIDIKTLFATSSIFLLIALLTIYYYGALPDTMVTHFGVNGEP
                     NGSMPKYMVTILTPLLFFCVHLFICILYDVKGIGKTPVIRVFKWLFPLLALIIQVSLL
                     WYNLGGELDYRRLVIGLLAVYYMVIGNYLPKEELDSKTNEIERKGRKQSGYLLMIGGL
                     LQLVSLLGSPTLSILVMILVGISVVSLSIYYAYLHFKTAS"
     gene            967..1374
                     /locus_tag="ACHU66_001543"
     CDS             967..1374
                     /locus_tag="ACHU66_001543"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR3389813.1"
                     /translation="MITYKQNPQLDFQAVLEIYDSVGWTNYTDRPTMLQKALEHSLLV
                     LAAFDGDRLVGLLRAVGDGHSIVFIQDILVLPTYQRQGIARQLLEQAIAYFPGIYQLH
                     LLTDNTEKTRSFYEAIGFTAVDSLDSVAYTYLK"
     gene            1497..2108
                     /locus_tag="ACHU66_001544"
     CDS             1497..2108
                     /locus_tag="ACHU66_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFR3389814.1"
                     /translation="MVTVLIVKAHPLDAQKSYALRALEEFQTRYASLHPEDQIEVVDV
                     FEDQIPALDKSLLEAMGAAKKGESISPDQAKQLDRYNALTQQFLMADKIVVVNPLWNL
                     NVPSQLVSWVNTINVAGLTFKYGPEGSIGLVKDKKLLHIQSNGGVYAGQDPAAQYIKS
                     IFEFLGFEDIHQVFIEGQSADPSQAQAIFEEAMGKIDAILEVF"
     gene            complement(2124..2396)
                     /locus_tag="ACHU66_001545"
     CDS             complement(2124..2396)
                     /locus_tag="ACHU66_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFR3389815.1"
                     /translation="MENKAYFYVLECADGSLYTGYTTDVEKRLATHNRGKGSKYTRGR
                     LPVSLLYQEAFASKQEAMSAEALFKKRSRQSKLDYIAEMTKKPRTK"
     gene            complement(2386..3135)
                     /locus_tag="ACHU66_001546"
     CDS             complement(2386..3135)
                     /locus_tag="ACHU66_001546"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFR3389816.1"
                     /translation="MQKIDLLDGERIDQLFSTDVQIIQNREVFSYSIDSVLLSRFPKM
                     PAQKGLIVDLCSGNGAVGLFASTRTKAQIIQVELQERLADMNRRSIALNGLEEQVSVI
                     NDDLANLPQYDLRSKVDLMLCNPPYFKVDKDSNLNESEHYLLARHEIATNLDSICQVA
                     QQVLKSNGRLAMVHRPDRFLDILDKLRTYKLAPKRIQFVYPKASKEANMLLIEAIKDG
                     SVDGLHILPPLVVHEENGDYTPAIREIYYGK"
     gene            complement(3578..3829)
                     /locus_tag="ACHU66_001547"
     CDS             complement(3578..3829)
                     /locus_tag="ACHU66_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389817.1"
                     /translation="MFKEFNKEYYRSLNFYLMILLFLGSIGEWLNKGPSLWLALLLFA
                     TVGTARSAFWSKEWMLRANWQIYLCIAFLLVGVWVEFFV"
     gene            complement(3839..4093)
                     /locus_tag="ACHU66_001548"
     CDS             complement(3839..4093)
                     /locus_tag="ACHU66_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389818.1"
                     /translation="MSKKFNKAFYRTWEFYLSLACILQVISAAIHGPFWFIHFLKLGV
                     GLYAGYLAFFKSRSWIKKTWQIYLTIGLIASWTIFYFFVL"
     gene            complement(4107..4373)
                     /locus_tag="ACHU66_001549"
     CDS             complement(4107..4373)
                     /locus_tag="ACHU66_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389819.1"
                     /translation="MKFQFNKHFYRYSAFYSMIFWTCFFLSDILLDPSQTPAWYSLLA
                     CLIIFINAIRVFFFKEEKELTRMGYIFYIGLIVVIIYLKFILGK"
     gene            4575..5666
                     /locus_tag="ACHU66_001550"
     CDS             4575..5666
                     /locus_tag="ACHU66_001550"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR3389820.1"
                     /translation="MTDYKIDTILAHTGINSDERTGALISPIHLSTTYQHPEFGQSTG
                     YDYTRTKNPTRASLETTLAAIEKADYALATSSGMAALVLLFNGFPVGSQVVAARDLYG
                     GSFRWFNEQESIGRFQFTYANTEEELIAAITEETDYVYLETPTNPLMVEFDIAKVSAI
                     AHAKGAKVIVDNTFYSPIYQNPLVLGADVVLHSATKYLSGHNDVLAGALMTNDQDLYD
                     KLFYDQNTSGPTLSPLDSYLLMRGLKTLSLRMERTTQNAQKIVAYLEKSPAVKQVYYT
                     GKGGMISVKVVDESRIPHILNTLKVFTFAESLGGVESLITYPATQTHADIPAEIRHSY
                     GLTDDLLRLSIGIEDAEDLIADLKTGLEG"
     gene            5670..6830
                     /locus_tag="ACHU66_001551"
     CDS             5670..6830
                     /locus_tag="ACHU66_001551"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFR3389821.1"
                     /translation="MTQYNFIDSPNRLSQHAEKWRKVETDKELLPLWIADMDFEPLPE
                     IRQVIRDYADHHVFGYPYASDSLYQSIIDWEDRQHGYKIERESILLIEGVVPALSVAI
                     QSLTEEGDAVLINTPVYPPFARTVKLNNRQLVTNSLVDVDGIFRLNLNQLEKDIVENQ
                     VKLYIFCNPHNPGGRVWTREEVLAIGRLCQKHGVILVSDEIHQDLTLYGHKHYSFNTV
                     AESFKEFSIILSSATKTFNIAGTKNSFAIIENPSIRKTFAKCQLINNQHEIPTIGLLT
                     TEAAFTYGDEWLKELKVILERNIDFVEEYLTTHTRIKVMKPQGTYLIWLDFSAYELEH
                     AELFDILHCKAKLILNDGLTFGKEGKHHARLNVAAPFNMIEEACQRLGKVFG"
     gene            complement(6862..7701)
                     /locus_tag="ACHU66_001552"
     CDS             complement(6862..7701)
                     /locus_tag="ACHU66_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR3389822.1"
                     /translation="MNILNIYNELDSHNFDLNVDHYGAEQCDKGYSFGPTIRDNFVLH
                     FITNGKGKILVDGKTSYLSAGDIFILPKDVSIFYQADDIDPWTYVWVGFSGSRAKDLL
                     NQSQLLENYYLHSNLESPILGYMHQINHVQMHPIPSITELVLIGYLNQLLAALIEEFP
                     SETLIKPETQTKHYVQQAIKMIHNHYAHPIKVSEIADFLALSRSYLYKIFKQETGYSI
                     KDYILQVKMNRSCQLLEDASRSITEIAYSVGYQDPLTFSAAFKNYFHMSPTEFRKTQK
                     NKD"
     gene            7807..>9927
                     /locus_tag="ACHU66_001553"
     CDS             7807..>9927
                     /locus_tag="ACHU66_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR3389823.1"
                     /translation="MNVIEIYNEKQIFHLKTREFSYIIQVLETGDLVHRYFGKKIEKF
                     SEGNKITYLDRSFSPSPITGDRTYSLDVLPLEYSSNGLGDFRTSALDVRNEFGVTLDL
                     KYKEYRLYKGKKELRGLPASFGNQEEVESLEIDLYDQLTDITVTLQYSVFEEASYLAR
                     SATIQTGKYPCKLEKVLSATLDFPHQDFIVHSLAGRYAYEKEWTQTPLTKGQYSIGSI
                     RGASSHSRTPFLALASPDVSEDKGDVYAAHLVYSGNFTAFVETTAMETSRLGLGLESH
                     YFSWQLDKDDRFQTPEVLLSYTDKGFTGMTQNSHHFITKHLIRSSFVNKPRPILINNW
                     EATYFEFTEEKILQLAQVASRAGIELFVLDDGWFGKRNNDESSLGDWKVNLDKLPNGL
                     NGLAERINELGMKFGLWFEPEMISIDSDLYREHPDWAIRTEGRLPIYSREQLVLDLTK
                     QEVCDYIIDSVSSILESANISYVKWDMNRNITNIPEGLANDQRFEFHHRYMLGLYRVL
                     DHLTKRFPDILFESCAGGGGRNDLGIMYYMPQAWASDDTDAIERLSIQEGTSLIYPPS
                     SIGAHVSAVPNHQVGRITPLATRGNVAMMGGAFGYELDLTKLSEKELDEISQQIETYH
                     SIRETIQFGQLYRLKKTSNIWAANYVSQDKNQVVFTFVKILAKPEAPLLHVRLKGLDP
                     DTLYGCPQLGETFYGDELMNIGLTM"
BASE COUNT         2994 a         1938 c         2126 g         2869 t
ORIGIN      
        1 aaaccttgaa ggcaatttct gctcctattc gtaggcaaat tctagatagt cttaagtcgg
       61 ggccgaagtc tgctggggaa attgtagaac aatttcaatt aacaggagca acagtttctc
      121 atcatttatc cactttgaaa aaagctggat taattttgga ggaaaagcag aaaaatttta
      181 tttattatcg tctaaactat acggtctttg aagaagtctt ggtttggatt gcaagttttg
      241 gagggcaaca agatgaagaa aattgacata aaaaccttat ttgcaacttc tagtatattc
      301 ctactgattg ctttattgac gatttattac tatggagcat tgccagatac catggtaacg
      361 cattttggcg taaatggtga gccaaatggc tctatgccca agtatatggt gacaattttg
      421 acaccgctac tattcttttg tgttcaccta ttcatttgta tactttatga tgtgaaaggg
      481 atagggaaaa caccggtcat tcgagtgttc aaatggcttt ttccgctact ggctctcatc
      541 attcaagttt ctttactctg gtataattta ggaggagaat tggattaccg taggcttgtc
      601 attggactgc tggctgtcta ttatatggtg ataggaaatt atttacccaa ggaggaattg
      661 gatagcaaga ccaatgaaat agaacgaaag ggaagaaagc agtcaggcta tctcttgatg
      721 attggtgggt tgttgcagtt agtaagtttg ttgggttcac caaccctatc tatccttgta
      781 atgatattgg ttggtatttc tgttgtaagt cttagtatct attatgctta tctgcatttt
      841 aaaaccgctt cataattgga gcagtttttg ttatactaaa tgtagtaaaa caggaggtaa
      901 gaccatgggc gttttaacaa tggacaaagc ctttgtattc taaaaaacaa tacaaaggag
      961 aaagacatga ttacttacaa acaaaatcct caactcgact ttcaagcagt ccttgaaatc
     1021 tatgattcag ttggttggac aaattatact gaccggccta ccatgttaca gaaggccttg
     1081 gaacatagtc tgctcgttct tgctgccttt gacggtgacc gtctagtggg tctcttgcga
     1141 gcagttggcg acgggcattc aatcgttttt attcaggaca tcttggtcct accaacctat
     1201 cagcgacaag gaattgcgcg tcagcttttg gagcaggcca ttgcctactt tccaggtatt
     1261 tatcaactcc atctcttgac ggacaatact gagaaaacac ggtctttcta tgaggcaatt
     1321 ggctttacag ccgttgatag cctggacagt gtcgcttaca cctatttaaa ataataaaac
     1381 aatctaaaaa agcagaggct gggagaccag ccttttgctt cgagcaaatg atttgaattt
     1441 caaatcaaaa agagtagaat ataattatca ataattagta acaaaggaga tgaacaatgg
     1501 taactgtatt aattgtaaag gcccaccccc tcgacgcaca aaagtcctac gccttgcggg
     1561 ctttggaaga gtttcaaact cgttacgcca gtttgcaccc agaagatcag atagaagtag
     1621 tggatgtttt tgaagaccag attccagcct tggacaaatc tttgttagag gctatgggag
     1681 ctgctaaaaa aggcgagagt atcagtcctg atcaagcaaa gcaattggac cgttacaatg
     1741 ccttgaccca gcaatttctt atggcggata agatagttgt cgtcaatcct ctctggaatc
     1801 tcaatgtgcc aagtcaattg gtatcttggg tcaataccat caatgtagca ggcctgacct
     1861 ttaagtatgg tccagaaggc tctatcggtt tggtcaagga caaaaagctc ctgcacatcc
     1921 agtccaatgg tggtgtttat gcaggtcagg atccagcagc ccagtacatc aagtctatct
     1981 ttgaattttt aggctttgaa gatattcatc aagtctttat cgaagggcaa tctgccgatc
     2041 caagccaggc tcaggctatt tttgaagaag ctatggggaa aattgatgca attttggaag
     2101 tattttaaat aaaaagcctc gtactatttt gtacgaggtt tttttgtcat ttctgcaata
     2161 taatctaatt tactctgccg actgcgtttc ttaaacaaag cctcggcaga catggcttcc
     2221 tgcttactag caaaggcttc ttgatagagc aagctgacag gcaagcgtcc gcgggtgtac
     2281 ttggaccctt tcccacgatt atgggtagca agccgcttct ccacatcagt cgtatagcca
     2341 gtataaagac tcccgtcggc acattccaaa acataaaagt aggccttatt ttccataata
     2401 aatctcacga atagccggag tatagtcgcc attttcctca tggacaacca gaggtggcag
     2461 gatatgcaga ccgtccaccg agccgtcctt gatagcctca atcagcaaca tattggcctc
     2521 cttgctggcc ttgggataga caaactggat gcgtttggga gccaatttgt aggttctcaa
     2581 cttgtccaaa atatctaaaa accggtcggg cctgtgaacc atggccaagc gaccattgga
     2641 cttgagcacc tgttgggcca cctgacaaat gctgtctagg ttggtcgcaa tctcgtgtct
     2701 agctaggaga taatgctcgc tttcattgag gtttgagtct ttatctacct tgaagtaggg
     2761 aggattgcag agcatcaaat caaccttgga gcgtaaatca tactggggca gatttgccaa
     2821 gtcatcattg ataaccgaga cctgttcctc caaaccattg agggcaatgg agcggcggtt
     2881 catatccgcc aagcgttcct gcaactcgac ctgaataatc tgagccttgg tgcgagtgga
     2941 ggcaaagaga cccacggccc catttccgct acaaaggtca acaatcagcc ccttctgagc
     3001 aggcattttt ggaaaccgcg aaagaagaac gctgtcaatc gagtagctaa atacctcccg
     3061 attttgaata atctgaacat ctgtcgagaa gagctggtca atccgctctc cgtctagtaa
     3121 atcaattttt tgcataggta tattatacca tctttgaatg aaaagcactg tttattttga
     3181 aagaaaagag gctgggcaaa aagccctggc ccacttctca gtgttcgtgt caacatctca
     3241 gcgcagtggt tgattgggtt taacagtcca gtggactgtt aaaggttgga gataggattt
     3301 gcgaagcaaa tctcagcaat tcgtgtttta cactccaaat ctgaccatta cgactgttgc
     3361 gaacaaagtt cgctctattt ccaacctcaa aaggttcact gaaccttttt gtaacgaacc
     3421 ttgttcgctc tatctccaac ctccaacagt cactactctg actgttggag ctatgcgggg
     3481 gtgggagtga aacagtctgg gaatagactg tttcagctca acaactggaa ataaggactt
     3541 gttgacgaac tctttttgac ttagtcgagt tcaactctta aacaaaaaat tctacccaaa
     3601 ctccaacaag caagaatgcg atgcagagat agatttgcca atttgccctc aacatccatt
     3661 ctttagacca aaaggcacta cgggcagtac caacagtagc aaagagcaag agtgcaagcc
     3721 agaggctagg tcccttattt agccactccc cgatacttcc tagaaacaac aatatcatca
     3781 gataaaaatt caaactccga taatattctt tattaaattc cttaaacata taaaatcctc
     3841 acagtacaaa gaagtaaaag attgtccaac tagcaatcaa tccaatcgtc agataaattt
     3901 gccatgtttt tttaatccag ctacgtgact taaagaaagc tagataacca gcataaagtc
     3961 cgactccaag cttgagaaaa tggataaacc aaaacggtcc atgtatagca gcagaaatca
     4021 cttgtaggat acaggcaaga gacaagtaaa attcccaggt ccgatagaat gctttattga
     4081 attttttaga catagtaagc ccctttctat ttacccaaaa tgaatttcag atagataatc
     4141 accacaatca atccgatata gaagatgtag cccatgcgag tgagctcctt ttcttccttg
     4201 aagaagaata cccgaatggc attgataaaa ataatcagac aggctagtag gctataccat
     4261 gcaggagttt gggaagggtc tagcaaaatg tctgatagaa aaaaacaagt ccaaaaaatc
     4321 atagaataga aggcgctata ccgatagaaa tgcttgttaa actggaattt catgacaatc
     4381 acctctcttt cttttattat atcaaataaa ttataggttt aaaagtattt attggtagat
     4441 ccagtatgga tgttgttata aagaaaaact ataacaaagc ttgctatttt tctataagta
     4501 tttttggata tttttcacac caaatctcct atttcatgct agactatatt gtaagttaga
     4561 ttggagtaga tgttatgaca gattataaga ttgatacgat tttagcgcat acgggcatca
     4621 atagcgatga gagaacaggt gccttgattt cccctatcca cctttcgacg acctatcaac
     4681 accctgagtt tggtcaatcg acaggttatg actacacgag aacaaaaaat ccgacacgtg
     4741 ccagtctaga gacgactttg gcagctattg aaaaggccga ctatgcccta gcaacgagct
     4801 cgggaatggc tgctctggtc ttacttttta atggttttcc tgtagggagt caagtggtag
     4861 cggcgcgtga tttgtacggt ggttccttcc gctggttcaa tgagcaagag agtatcggta
     4921 gatttcaatt tacctatgca aacaccgagg aggagctgat tgcagccatc accgaagaga
     4981 cagactatgt ttatctggag acaccgacta acccactcat ggtggagttt gacatcgcta
     5041 aggtttcggc tatcgcccat gccaaaggtg ctaaggtgat tgtcgataac actttttaca
     5101 gccctattta tcaaaatcca cttgttctag gagcggacgt tgtcctacat tcagcaacca
     5161 agtacctatc tgggcataat gatgtactag ctggcgcttt gatgaccaat gatcaagacc
     5221 tatatgataa gctcttttat gaccaaaata catcgggtcc aaccctatct ccactggatt
     5281 cttacttact catgcgtgga ctcaaaaccc tctcgcttcg aatggagcgg acgacacaaa
     5341 atgctcagaa aattgttgcc tatttggaga agagtccagc agtcaagcaa gtttactaca
     5401 caggaaaagg tgggatgatt tcggttaagg tggtagatga aagtcggatt ccccatattc
     5461 tcaacaccct gaaagtcttt acctttgctg aaagtctggg tggagtagag agtctgatta
     5521 cctatccagc cactcagacc cacgcagaca ttcctgctga aatccgccat tcttatggat
     5581 tgacagatga cttgcttcgt ttatctattg gtatagagga tgcggaagac ttgattgcag
     5641 atttaaagac aggcttggag ggataagata tgacacagta taattttata gatagtccga
     5701 atcgtctatc ccagcatgcg gaaaaatgga gaaaagtaga gacggataaa gaactcttac
     5761 ccttgtggat agcagatatg gactttgagc ctctaccaga aattcgccaa gttatccgtg
     5821 actacgcaga tcatcatgtt tttggttatc cttatgctag tgacagcctc tatcagtcta
     5881 ttattgactg ggaagataga cagcatggct ataagattga aagagagtct atcctactca
     5941 tcgaaggagt tgttcctgcc ttatctgtgg ctattcaatc tttgacagaa gaaggggatg
     6001 cagtcctaat taatacacct gtctatccac catttgcgcg gactgtaaaa ttaaacaatc
     6061 gccagcttgt taccaattca ctagttgatg tggatggtat ttttaggctt aatcttaacc
     6121 agcttgaaaa ggacattgta gagaatcaag tgaaactcta tattttctgt aatccccaca
     6181 acccaggtgg tagagtttgg acaagagagg aagtgttggc cattggacgt ctttgtcaga
     6241 aacatggtgt tatccttgtt tcagatgaaa tacatcaaga tttgaccttg tatggtcaca
     6301 agcattattc ctttaacaca gtggctgaaa gttttaaaga gttttcaatc atcttatcat
     6361 ctgcgaccaa gaccttcaat atcgctggta caaaaaatag ctttgcaatc attgaaaatc
     6421 caagtattcg caagactttc gctaagtgtc aattgataaa taatcagcac gagattccaa
     6481 cgattggctt gttaaccaca gaagctgctt tcacttatgg agatgagtgg ttgaaggagt
     6541 tgaaggttat tttggagaga aatatagact ttgttgagga gtatttgaca acccatacaa
     6601 ggatcaaggt catgaagccc cagggaacct atctcatctg gcttgatttt tctgcctatg
     6661 aattagagca tgcggagctc tttgatattc ttcattgtaa agcgaagttg attttgaatg
     6721 atggtttgac ctttggtaag gaaggtaagc accatgcacg tctgaatgta gcagcaccat
     6781 tcaacatgat tgaagaagcc tgtcagcgac ttggtaaggt ttttggataa agaaataagg
     6841 actaggtgtg actaaactca cctaatcctt atttttttgt gtttttctaa attctgtcgg
     6901 gctcatatgg aaataatttt tgaaggccgc gctgaaggtg agagggtctt ggtatccgac
     6961 agaataggcg atttcagtga tactccgaga agcatcttct aatagttgac aagaacgatt
     7021 catttttact tgtaaaatat aatctttgat agaatagcca gtttcctgct taaaaatttt
     7081 atagagatag cttcggctta aagctagaaa atctgctatt tcagaaactt taattggatg
     7141 ggcatagtgg ttatggatca tcttgatggc ttgctgaaca tagtgcttgg tttgtgtttc
     7201 aggcttaatt aatgtttcac tgggaaattc ctctatgagt gctgctaaga gttggttgag
     7261 gtagccaatt aagaccaatt ctgtgataga ggggatggga tgcatctgaa catggttaat
     7321 ctgatgcatg tagcctagaa ttggtgactc tagattggag tggaggtagt agttttctag
     7381 cagttgactt tgattaagta ggtcttttgc tcgtgaacca ctaaaaccaa cccatacata
     7441 ggtccacggg tctatatcat cagcttgata gaaaatggaa acatcctttg gaagaataaa
     7501 tatatccccg gcagaaagat agctagtctt accgtctaca agaatcttcc cttttccatt
     7561 tgtaataaaa tgcaggacaa aattatcacg gatagtagga ccgaaggaat agcccttgtc
     7621 acattgttcg gcaccgtaat ggtctacatt taggtcaaag ttgtgactat caagctcgtt
     7681 atagatattc agaatattca tttcaatcct ccttaggaaa cattataaca tgttttaaat
     7741 acaaaagacc attaaataaa gcgttttcat tttataaaat aagagcataa agaagagata
     7801 ggagagatga atgtgattga gatttataat gaaaaacaga tttttcacct gaaaacaaga
     7861 gaattctctt atatcatcca agtgttggaa acaggtgatc tggttcatcg ttattttggg
     7921 aaaaaaatcg aaaaatttag cgagggaaac aaaataacat atttagatcg atcgttttct
     7981 ccgagtccaa ttactgggga taggacatac tcattggatg tcctcccact cgaatattct
     8041 agcaatggtc tgggagattt tagaacctca gcattagatg ttcggaacga atttggagtc
     8101 actcttgacc taaaatacaa agagtatcga ttatacaagg ggaaaaaaga actgagggga
     8161 ttaccagcaa gttttggaaa tcaagaagag gtagagagtc ttgaaattga tctttatgat
     8221 cagttgacag atattaccgt aacacttcag tattctgttt ttgaagaagc ttcctatctc
     8281 gcacggtcag ctacaattca aactggcaaa tatccatgca aactagagaa ggtcttgtct
     8341 gcaacgcttg attttccaca tcaagatttc attgttcaca gcttggcagg acgttatgct
     8401 tatgaaaaag agtggacaca gaccccattg acaaaaggtc agtattcgat tggtagcatt
     8461 cgaggtgctt caagtcactc aagaacaccc ttcttagcac tagcctcacc cgatgtaagt
     8521 gaggacaagg gcgatgttta tgcggcccac cttgtctata gcggcaactt tacagcattt
     8581 gtcgaaacga cagccatgga aaccagtcga ttgggactag gattggaaag tcactatttc
     8641 tcatggcaat tggataagga tgaccggttt caaactccag aagttctctt atcatatact
     8701 gataaaggat ttactggcat gacacaaaat agccatcact ttatcacaaa acacctcatt
     8761 cgatcatcgt ttgtaaacaa accaaggccc attttaatca acaactggga agcgacctat
     8821 tttgaattta cagaagaaaa gattttacag ttagctcagg tcgcaagtcg agctggtatt
     8881 gaattatttg ttctggatga tggttggttt ggtaaacgaa acaatgatga aagttcattg
     8941 ggcgattgga aagtcaattt ggataagttg ccaaatggtt tgaatggttt agcagagcga
     9001 ataaatgagc ttggtatgaa atttggactg tggtttgaac cagagatgat ttctattgat
     9061 agcgatcttt atcgtgagca tcctgattgg gcaattcgca cagaaggacg cttaccaatc
     9121 tacagccgag aacaattagt tttggatttg accaagcagg aagtctgtga ctacatcatt
     9181 gatagcgtct cttccatatt agaatcagcc aatatttctt atgtaaaatg ggatatgaac
     9241 cgtaatatca ccaatatccc tgaaggttta gcaaatgacc agcgctttga gttccaccat
     9301 cgttacatgc taggtctcta ccgtgtatta gaccacctta ccaagcggtt cccggatatt
     9361 cttttcgaat cctgtgcagg cggtggaggt cgtaatgatt tgggaatcat gtattacatg
     9421 ccacaagctt gggcaagtga tgatacagat gcgattgagc gcttatctat tcaagaaggt
     9481 actagtttga tttatccccc ttcctcaatt ggtgcacatg tttctgcagt tccaaaccat
     9541 caggttggtc ggatcacacc tcttgctaca cgaggcaatg tggctatgat gggaggagca
     9601 tttggttacg agctggattt aacgaaactt tcggaaaagg aattggatga aatcagtcag
     9661 caaatcgaaa cctatcactc cattcgtgaa actatacaat tcggtcagct ctaccgtctg
     9721 aaaaagacga gcaatatctg ggctgccaat tatgttagtc aagataagaa tcaagtggtc
     9781 tttacatttg taaaaattct tgccaagcca gaagcgcctt tgcttcatgt tcggttgaag
     9841 gggctagacc cggatacctt gtatggatgc cctcaattgg gagaaacatt ctacggggat
     9901 gaactgatga acattggtct tacaatg
//
LOCUS       DAZQCJ010000072         9793 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000072 DAZQCJ010000000
VERSION     DAZQCJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9793
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.073"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            2..766
                     /locus_tag="ACHU66_001554"
     CDS             2..766
                     /locus_tag="ACHU66_001554"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HFR3389824.1"
                     /translation="MKRLLRRIRPTKPLKELTWIDLIIITTILCGNAIYTSTMQWIAS
                     LSATETVETGVLSFSPADNWWALANQGKLFLFALVYLLIRNYDFKQLKVKLEWRVLLW
                     GPLIFIGAGLISDLAFTAFSYIPGLSGGYNFLGYLPYYDWNIMTVLNRFLAVDYSTVI
                     YSLFNGFYEEFFFLGLLLSTDKKKRSLVLLFSTIVRISFHTYQGMVSALVIGVAFGLF
                     YYYMYTRKNDNLLPYFLGHALADMVGTSFFSLFIAG"
     gene            1009..1260
                     /locus_tag="ACHU66_001555"
     CDS             1009..1260
                     /locus_tag="ACHU66_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR3389825.1"
                     /translation="MKQIKNRLKKHMFWIGLISLVLMLVDNVLVCYFNIECSRTLNQI
                     EQTICIILSILVFLGVLNNKPDVKVEDIMKNGMSSKNQK"
     gene            1611..2366
                     /gene="ldcB"
                     /locus_tag="ACHU66_001556"
     CDS             1611..2366
                     /gene="ldcB"
                     /locus_tag="ACHU66_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase LdcB/DacB"
                     /protein_id="HFR3389826.1"
                     /translation="MIMKRTILLCLPTILLLAACHQNINQANTTKNSSTVVSTTSSQS
                     ASTTSEQSTSSSTSAETNQEVTSTETQVNFNGSYYSVQGKYGEVIIVNKKHPLAADYA
                     PGENPEALAAFQRLIADMQALGFGISNNYSGFRSYETQASLYQSYVNQEGQAAADRYS
                     ARAGYSEHQTGLAFDLLDTSGNLLEEPNASQWLATNAHLYGFIVRYLPGKESSTGYMA
                     ESWHVRYIGQEATDIYNSGLTLEEYFGVPGGDY"
     gene            2455..2911
                     /locus_tag="ACHU66_001557"
                     /pseudo
     CDS             2455..2911
                     /locus_tag="ACHU66_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(2934..4544)
                     /locus_tag="ACHU66_001558"
     CDS             complement(2934..4544)
                     /locus_tag="ACHU66_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR3389827.1"
                     /translation="MSKSIIWELVKINILYSNPQLLASVKKKQSKRKDASFSAYKSVL
                     LQQVFMVLSFGLLYSVFFMGVDYSQSIGFFSLQLALFTIIAIVYGFTGFFSVFYDSKD
                     TKLYLALPLKPQEVFLAKILSAQGMVLPFLMPCLSLLLITYWQIGGPLLALASIPSFL
                     ILWILANLVNLIIMHFVGEVLTKSPRKTLISTILMTGSSLLAFAALMFLQSQQTVSLE
                     SGGFVDFPIIPIFAGFHYLVSHTISLETLLHFGLPFILMIGLIVFAFKTVIPNYFNQV
                     LAIENASTKRTNNAKSAKIPSNLNQALVKHHLSTLKDSNLMVQTMIQPIIMGVALFPS
                     FSRFTENGGLSSVEWDFFGIALLAGFLLGSLFAGSTTFIGVAMSLEKENYHFIRTLPI
                     NFKKFIIQKFWVLAAVQFALPTLLYFALALGFMKVKLILAIFFTVGIIISALLTGQIY
                     YWRDQRLLMLNWQNSNQLFGRGAGQWLIAGSIIGTMFLGAIILVVSIILAGTIGAAYV
                     SSALMTLLFVLSAALQLYIYKAYWQKLK"
     gene            complement(4541..5284)
                     /locus_tag="ACHU66_001559"
     CDS             complement(4541..5284)
                     /locus_tag="ACHU66_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3389828.1"
                     /translation="MIQFDHITKTYGETIALDDLNLTIESGEIFGLIGHNGAGKTTTI
                     SLLTSIIEASYGTISVDGLNLEENRDEVKKRIAYVPDSPDIFLHLTAFEYWHFMGKVY
                     GVEKDTVDDRIAKLSSLFDMTAHQHEPIDSFSHGMRQKTIIIGALIPNPDIWILDEPL
                     TGLDPQASFDLKQMMKEHAQSGHTVLFSTHVLSVAEQLCDRIAILRKGQLIFVGSLDE
                     LKSQYPDKDLETIYLELAGRQQVKAGEEV"
     gene            5524..7308
                     /gene="uvrC"
                     /locus_tag="ACHU66_001560"
     CDS             5524..7308
                     /gene="uvrC"
                     /locus_tag="ACHU66_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR3389829.1"
                     /translation="MNDLIKHKLELLPDSPGCYLHKNKYGKIIYVGKAKNLRNRVRSY
                     FRGAHDTKTEALVAEIADFEFIVTDSNIEALLLEINLIQENKPRYNIMLKDDKSYPFI
                     KITKERHPRLIITRQIKKDGGQYFGPYPDVGAANQTLKLLERLYPFRKCKLPENKYCL
                     YYHLRQCLAHGDNMPSLSDYDKMVKEVAQFLTGHDDKIIKEVQDKMKAAAGNLEFEKA
                     AEYRDVLQSISTLRTKQRVMAHDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERNVNL
                     FPYYNDADEDFLTYIGQFYRDQQHLIPSEVLIPQDIDQEAVEALVDTKVIKPQRGEKK
                     QLINLATKNARVSLEQKFNLLEKNLEKTYGAVENIGQLLGIPTPVRIEAFDNSNIMGT
                     SPVSAMVVFENGVPNKKEYRKYKINTVEGPDDYASMREVLQRRYSRVLRDDLTPPDLI
                     IIDGGQGQVNVAKQVIEQELGMSIPIAGLQKNDKHQTHELLFGNPLEVVELPRNSQEF
                     FLLHRIQDEVHRFAIEFHRQVRSKNSFSSKLDGIEGLGPKRKQALMKHFKSIGNIQSA
                     SLQEIAEAGVPYKVAEKVKESLSSKDEI"
     gene            7365..7493
                     /locus_tag="ACHU66_001561"
     CDS             7365..7493
                     /locus_tag="ACHU66_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFR3389830.1"
                     /translation="MAFGEEKHKKTTFEKVTLVIVVVMVVATLAGLILPAINALMN"
     gene            7697..9010
                     /gene="obgE"
                     /locus_tag="ACHU66_001562"
     CDS             7697..9010
                     /gene="obgE"
                     /locus_tag="ACHU66_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFR3389831.1"
                     /translation="MSMFLDTAKIKVKAGKGGDGMVAFRREKYVPNGGPWGGDGGHGG
                     NVVFVVDEGLRTLMDFRYNRRFKADDGEKGMTKGMHGRGAEDLIVRVPQGTTVRDADT
                     GKIITDLVENGQEFVIAHGGRGGRGNIRFATPKNPAPEISENGEPGEERNLELELKVL
                     ADVGLVGFPSVGKSTLLSVITAAKPKIGAYHFTTIVPNLGMVRTKSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVLDMSASEGRDPYEDYVAINNELETYNLRLME
                     RPQIIVANKMDMPEAAENLEEFKKKLAANYDEFEELPQIFPISGIAHQGLKNLLEATA
                     ELLEKTPEFLLYEESDFQEEEAYYGFNPDEPEFDISRADDASWVLSGDKLEKLFTMTN
                     FDRDESVMKFARQLRGMGVDEALRARGAKDGDIVRIGKFEFEFVD"
     gene            9384..9548
                     /locus_tag="ACHU66_001563"
     CDS             9384..9548
                     /locus_tag="ACHU66_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389832.1"
                     /translation="MGDKPISFKDKDGNFVSAADVWNAEKLEELFNKLNPNRKLRLER
                     ERLAREQEKE"
BASE COUNT         3006 a         1770 c         2171 g         2846 t
ORIGIN      
        1 agtgaaaaga ttactgagac gcattcgtcc aacaaagccc ctaaaagaac tcacttggat
       61 agatttgatt attatcacaa cgattttatg tggtaatgct atttatacct caaccatgca
      121 atggatagca tcactttccg caacagaaac cgttgaaaca ggagttctgt cgtttagtcc
      181 agccgataat tggtgggctc tagccaatca aggaaaatta ttcctgtttg ccctggtgta
      241 tttattgatt cggaattatg atttcaagca gctaaaagta aaattagagt ggagagtttt
      301 gctctgggga ccactgattt tcatcggtgc tggtctgatt agcgatttgg catttacagc
      361 tttttcatat attccaggtc tgtctggtgg gtataatttc ctaggatact tgccttacta
      421 tgactggaat ataatgacag tgctcaaccg tttcctagcc gttgactaca gcacagttat
      481 ctattccttg tttaatggct tctatgaaga gttctttttc ttaggcttat tgttgagtac
      541 agataagaaa aagcgttcgc tggtcttact cttttcaaca attgtccgta tatctttcca
      601 tacctatcaa ggaatggtat ctgctcttgt tattggtgta gccttcggcc ttttctatta
      661 ctacatgtac acaaggaaaa atgacaattt attgccatat ttcttaggac atgctcttgc
      721 ggatatggtt ggtacaagtt tcttctcatt gtttattgca gggtaaacaa tgagaagaac
      781 ggacatcaag ctaatcggat aaaatttctt tctccctaca gctgacaaac ggatgatgat
      841 acgtagtttc tatcaactcg gtatcatcac ccgttttatt gtgctgatat atttaatctg
      901 ttcagtcaaa tttcgattgc tttatcatat tttttatccc atattttttt gaattaatgg
      961 tttatttatg ttaaaatgta tttttataca tcaaaaagga gtatagatat gaaacaaata
     1021 aagaatcgtt tgaaaaaaca tatgttttgg attggactaa tttcgttggt attgatgctc
     1081 gttgataatg tcctggtttg ttatttcaat attgaatgta gtcgtacttt aaaccaaatc
     1141 gagcagacta tttgtattat cctcagtatt cttgtatttt taggagtgct aaataataaa
     1201 ccggatgtga aggtagaaga catcatgaaa aacggtatgt catctaaaaa tcaaaaataa
     1261 tgtttgttta gaaactactg atgaggtatc tagttgtact tacagttcag tatctctagc
     1321 taagatggat ttttcaaaat atagtcagta tctcctaaaa aactattttg tatataaatc
     1381 actttacttg tgaatacgac tatttaacat catatatgtc caccagtcga aaggctggtg
     1441 tttctattta tgacatgtat gttatcagga atgccactat ttgaaaggaa aagaatcgag
     1501 agaaattcaa tgaatactaa aaatatcaat agtagttaaa atcataagcc gtcattagac
     1561 ggggtcttat gattgtgcta caatagacat aattataaga gaacggggtg atgataatga
     1621 aacgtactat tctattatgc ttgccgacaa tactgttact agcagcatgt catcaaaata
     1681 taaatcaggc caatactaca aaaaattcgt caacagttgt ttcgactact tcaagtcagt
     1741 ctgcttcaac aacttcggag cagtcaacaa gcagctctac atcggcggaa acgaatcaag
     1801 aagtaacaag tacagagact caggtaaatt ttaacggttc ctattatagt gtacaaggaa
     1861 aatatggcga agtgattatt gtaaataaaa agcatccgct tgcagctgat tatgcaccgg
     1921 gcgaaaatcc tgaagcctta gcagcttttc aacgattaat agcagatatg caggcgctag
     1981 gatttggtat ctccaataac tatagtggat ttcgttccta tgaaactcag gcaagtctct
     2041 accagtctta tgtaaatcag gaaggacaag cagcggcaga taggtattct gcaagagctg
     2101 gatatagtga acatcagaca ggattagcct ttgatttatt ggatacaagt ggtaatttat
     2161 tagaagagcc taatgcttct cagtggttgg ctacgaatgc acatctatat ggctttattg
     2221 tccgctatct tccaggaaaa gagtctagca caggctatat ggcagaatcc tggcatgtgc
     2281 gatatattgg tcaagaagcg acagatattt acaattcagg cttgaccttg gaagaatatt
     2341 ttggtgtacc aggtggagac tactaatttt acatttagtc agaaataagc cgaaaaattt
     2401 gtccgtataa ctaactcgtg atataataaa ccatcataat tttggaggtc catcatgctt
     2461 aaagcaatgg aagtatactt ggttactaat ggtaatggtt tggaagccat cgagttttat
     2521 aaaaatgctc ttggggcaac agttgagcaa gtaaatctat ttaaagattt cttaccagac
     2581 tgccctgctg aactagaaaa ttacgtgatg aatgcacagt ttcgtttgaa tggtcagcgt
     2641 tttatgttgt cagacaacaa cccagaaatg ccttatacag taggtgacaa tattacagtt
     2701 gcgcttatca cagacgatgc tgagacagcg caggaactat acagcaagtt atccgtagat
     2761 gcaactgcta tcaatatgga acttcaagat ggaacttcaa gcagttcctt ggtctcctgc
     2821 ctatggtaat gtaacagaca agtttggtat ctcttggcaa atcaatgctg aagtagaagg
     2881 ctacggtcag gaatattacg cagaaaacta aaatagattg gacaatgtcc aatctatttt
     2941 agtttttgcc aataagcttt gtagatataa agttgaagag cagcagatag aacaaatagt
     3001 aaggtcatca aggctgagct tacataagct gcaccaatag tgccagctaa tatgatgctg
     3061 acaactagaa taatggctcc gagaaacatt gttcctatga tcgatccagc aattagccat
     3121 tgacccgctc cgcgaccaaa taactggtta ctattttgcc agttgagcat gaggagtcgt
     3181 tggtcacgcc agtaataaat ctgaccagtt agcagggctg aaatgatgat gccgactgta
     3241 aagaagattg ctagaatgag tttgactttc atgaaaccta gagccaaagc aaagtaaagt
     3301 agagttggta gtgcgaattg tacggcagcc aatacccaga atttttggat gataaatttt
     3361 ttgaaattta taggaagtgt tcgaatgaaa tgataatttt ccttttccaa tgacatggct
     3421 actccgatga aggttgtaga accagcaaat agactgccaa gcaagaatcc agctaataga
     3481 gcaataccaa agaaatccca ttcaactgaa cttagaccac cattctccgt gaaacggcta
     3541 aaacttggaa aaagcgcaac tcccatgatg attggttgta tcatggtttg gaccatgagg
     3601 ttgctatctt taagagtaga taggtggtgt ttcactaaag cctgattgag attgctagga
     3661 atcttggcag acttagcatt attggtccgt tttgtcgaag cattttcaat cgccaaaact
     3721 tgattaaagt agttgggaat gacagtttta aacgcaaaca caatcagtcc aatcatcaaa
     3781 atgaaaggaa gtccaaaatg aaggagtgtt tctagtgata tagtatggct gaccaagtaa
     3841 tggaatccag caaatatagg tatgatagga aaatcaacaa agccaccaga ttcgagtgaa
     3901 acggtctgct gggattgcag gaacatgagc gcagcaaaag ccagcaagga agaacctgtc
     3961 atcaggattg tagaaatcaa tgttttacga gggctttttg tcagaacttc cccaacaaag
     4021 tgcatgataa tcagattgac taaattagct aaaatccaga gaatcaaaaa ggaaggaatg
     4081 gaagctaagg cgaggagcgg accgccaatt tgccagtaag tgattagaag taaagacaga
     4141 cacggcatga ggaagggtag gaccatccct tgtgcagaga ggattttagc gaggaagact
     4201 tcttgtggct tcaggggaag tgctaaatat aactttgtat ccttactatc gtagaaaacg
     4261 gagaagaaac ctgtaaaacc gtagacaatg gcaataatgg taaagagcgc tagttgcaga
     4321 gaaaagaaac cgatagactg actataatct actcccatga aaaagacaga atagagaagt
     4381 ccgaaactca aaaccataaa tacttgttgc aaaaggacac ttttataggc tgaaaaggag
     4441 gcgtctttgc gcttactttg tttcttcttg acggaagcta gaagttgagg attggaataa
     4501 agaatattaa tctttactag ttcccaaatg atggatttac tcatacttcc tcacctgcct
     4561 tcacttgttg gcgtccggct aattccaaat agatggtctc caaatcttta tctggatact
     4621 gtgatttgag ttcatcaaga cttcctacga aaatgagttg cccttttcgg agaatagcaa
     4681 tgcggtcaca gagttgttca gctactgaaa ggacgtgtgt agaaaataga acggtgtgac
     4741 cagattgagc atgttctttc atcatttgtt ttaggtcgaa agaagcctga ggatccagtc
     4801 cagttagggg ttcatccaaa atccaaatat cagggttagg gatcaatgca ccaataataa
     4861 tggttttctg acgcataccg tgtgaaaaac tatcaatagg ctcatgctgg tgcgctgtca
     4921 tatcaaatag actagatagt ttagcgatac ggtcatcaac tgtatccttt tctacaccat
     4981 agaccttgcc cataaagtgc cagtattcaa aagctgttaa gtgtaggaag atgtctggcg
     5041 aatctggaac ataggctatc cgttttttga cttcatcgcg gttctcctct aggtttaaac
     5101 catcaacaga gatagttcca taactagctt cgattatcga agtcagtaga ctgattgtcg
     5161 ttgtttttcc tgccccattg tgaccaatta aaccgaaaat ctcgccgctt tcaattgtca
     5221 aatttaggtc atcaagtgcg attgtttcgc cgtatgtttt tgtgatatgg tcaaattgta
     5281 tcataataac ctccttgtta ttcgtgaact aaaagtaggg gcttgtaggt attccaagcc
     5341 ttattccaaa tgttcttcga ataatatata caattaatta catttattat actgctgaat
     5401 tagtacaaaa gcaatagttt ctatcaatat ttttgtagta tggatacgga tatatttcaa
     5461 gaagtcgagt gtaaaaagga atgcaataag ctcgattatc agaaaatttg ctataataga
     5521 cctatgaacg atttgatcaa gcataaattg gagttactac cagatagccc tggctgttac
     5581 ctccataaaa acaagtacgg taagattatc tatgtgggca aggccaaaaa cctacgcaat
     5641 cgggtgcggt cttattttcg tggtgcccat gataccaaga cagaggctct agtggctgaa
     5701 attgccgatt ttgaatttat cgtgacggat tccaacatcg aggccctctt gctagaaatc
     5761 aatctgattc aggaaaacaa gccacgctat aacatcatgc tcaaggatga caagtcttat
     5821 cccttcatca agattaccaa ggaacgtcat ccgcgactaa tcattactcg gcagattaag
     5881 aaggatggtg gtcagtattt tggtccctat ccagatgtgg gtgcggccaa ccagaccctc
     5941 aaactattgg agcgcctcta tcctttccgc aagtgtaaat tgcctgagaa caagtattgt
     6001 ctttattatc atttgcggca atgtttggct catggagaca atatgcccag cctgtctgac
     6061 tatgacaaga tggtcaagga agtggcccag ttcttgacag gtcatgatga taagattatc
     6121 aaggaagtgc aggacaagat gaaggcagct gctggcaatt tggaatttga aaaggctgcg
     6181 gaataccggg atgtcctcca atccatttca accctgcgga ccaagcaacg tgtgatggct
     6241 catgacttgc agaatcgaga tgtctttggc tactacgtag acaagggctg gatgtgtgtg
     6301 caggttttct ttgttcggca ggggaaactg attgagcgga atgtcaatct ttttccatac
     6361 tataatgatg cggacgaaga ctttttgacc tacatcggtc agttttacag ggaccagcaa
     6421 catttgattc cctcagaggt cttgataccg caggatattg accaagaagc cgtagaagct
     6481 ctggtagata ccaaggtcat caagccccag cgtggcgaga agaagcaact catcaatctg
     6541 gcaaccaaga atgcgcgtgt cagtctggag cagaagttta atcttttgga gaaaaatctg
     6601 gagaagacct acggtgcggt ggagaatatc ggtcagcttt tggggattcc gacgcctgtg
     6661 cggattgagg ctttcgataa ctccaatatt atggggacta gtcctgtgtc ggctatggtt
     6721 gtctttgaaa atggggtgcc taataaaaaa gaataccgca agtacaagat taatacggtt
     6781 gaggggccag atgattatgc ctctatgcgt gaagtgctgc aacgccgtta tagtcgggtc
     6841 ttgagggacg atttgacacc gcctgatttg attatcattg acggaggtca ggggcaggtc
     6901 aatgtcgcca agcaggtcat cgagcaggag ttgggtatgt ccattcccat tgcaggtttg
     6961 cagaagaatg acaagcacca aacccacgaa ttgctctttg gcaatccgct ggaagtggtg
     7021 gagttgccac gtaattctca ggaatttttc ctcctgcatc ggattcaaga tgaggttcac
     7081 cgctttgcta ttgagtttca tcgtcaggtt cggtcaaaaa attccttctc atcaaaattg
     7141 gacggcatag aaggcttggg accaaaacgc aagcaggctt tgatgaagca tttcaagtcc
     7201 attggcaata tccagtcagc cagcttacaa gaaatcgcag aagcaggagt gccttacaag
     7261 gtggcggaaa aggtcaagga aagcctttct tcaaaggatg agatttgaaa acattcccat
     7321 aatgtgttag aataaactat caatgactaa ataaggagaa agcaatggct tttggtgaag
     7381 aaaaacacaa aaaaacaaca tttgagaaag tgactttagt aatcgttgtc gtgatggttg
     7441 tcgcgacatt ggctggcttg attttaccag ctattaatgc attgatgaat taagaacctg
     7501 tatttacagt tcggcacttt tatctaaggt tggtttttaa gctgttcatc gttagttttt
     7561 tcaaaataca gtcagtattc tctcaaagaa cgaccttgat tagctaaaaa acttttctta
     7621 tatataagca ctttgcttgt gaatagaggt ctaaaacgct tttaagcgtt tttttctagg
     7681 aaagaatagg taaattatga gtatgttttt agatactgcc aagattaagg tcaaggcagg
     7741 gaaaggtggc gatgggatgg ttgccttccg tcgtgaaaag tatgtaccaa acggcggtcc
     7801 ttggggcggt gatggtggtc atggtggaaa tgttgtcttc gttgtagatg aaggcttgcg
     7861 gaccttgatg gacttccgtt acaatcgtcg ctttaaggct gatgatggtg aaaaagggat
     7921 gacaaaaggt atgcacggtc gaggagcgga agatttaatt gttcgtgtac cgcagggaac
     7981 aactgtgcgt gacgctgata caggtaaaat cattacagac ctggttgaga atgggcagga
     8041 atttgtcatt gcccacggtg gtcgtggtgg tcgtggaaat attcgttttg cgacacctaa
     8101 gaatccagcg cctgagattt ctgaaaatgg agaaccaggt gaggagcgca atcttgaatt
     8161 agaattgaaa gtgttggctg atgtaggtct tgtaggtttc ccatcagttg gtaagtcaac
     8221 gcttctcagt gttattacag cagctaaacc taaaattggt gcctaccatt ttacaaccat
     8281 tgtgccaaat ttgggaatgg ttcggaccaa atctggcgag tcatttgctg tggcagactt
     8341 accaggtttg attgagggtg ctagtcaagg tgttggattg ggaacgcaat tccttcgtca
     8401 cattgagcgg acacgtgtta tcttgcatgt tttggacatg tcagcaagtg agggtcgtga
     8461 tccttatgaa gattacgtag ccatcaacaa tgaattggaa acctacaatc ttcgtctcat
     8521 ggagcgtcct cagattatcg ttgccaacaa aatggatatg ccagaagcgg cagaaaatct
     8581 ggaagagttc aagaaaaaat tggcagccaa ctatgatgag tttgaagaat tgccacagat
     8641 tttcccaatt tctggtattg ctcaccaagg tttgaaaaat cttttggaag ccacagctga
     8701 gttgctagag aaaacgcctg aattcttgct ctacgaggaa tcagatttcc aagaagaaga
     8761 ggcttactat ggctttaacc cagatgagcc agaatttgac atcagccgtg cagatgacgc
     8821 aagctgggtt ctatctggtg acaaactaga aaaactcttc accatgacca acttcgatcg
     8881 cgacgaatct gtcatgaaat ttgctcgtca gttacgtgga atgggtgtcg atgaagccct
     8941 ccgtgcgcgt ggtgccaaag acggcgacat cgtccgtatt ggcaagttcg aatttgagtt
     9001 tgtggattaa cgtagcagtt tgactgcaag ttaacttgct agtcaaacta tgccaaatcc
     9061 tatgggacct tggctagcta ccttactaac ttcacccaaa tggtagtatc gaccatttgg
     9121 gctccgtgtc gtaacgttgg aaacacactt aaatcctaaa ggacttgatg tgatacggta
     9181 gccgctatgg cggactacct accaacctta ctaattccgc acaggcagtg gtatcgactg
     9241 cctgtactcc atatcgtaac ctgtccggta agttagtcta gtatgaactt agaaattcga
     9301 gagcagacta tagctttaca aatttcgttc agttggtgac agtcgtttgt gagcaatcga
     9361 aaaaaaaata gttggaggtc tgtatgggtg ataagccaat atccttcaag gataaagatg
     9421 gaaattttgt ttcagcagcc gatgtttgga atgctgaaaa attagaagaa ctctttaaca
     9481 agcttaatcc caatcgcaaa ctccgtttgg aacgtgagcg tttggcaaga gagcaagaaa
     9541 aggaataacg tcaaaatctg cttgttgtat cagaaaacag gcagattttt ttaaaattta
     9601 ttgactattt attattggct atatgtataa gaatacaaga tatggagcta atatttttgc
     9661 ggtgtcggta attttataga aattctcaaa agtgaaattt cttaaaaaat atgtaaaaaa
     9721 ctatgaatat actggtttct tctcgaattg aagcgaattc ataatgtttt tttgaggaat
     9781 tttataatat tac
//
LOCUS       DAZQCJ010000073         9662 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000073 DAZQCJ010000000
VERSION     DAZQCJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9662
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.074"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            69..425
                     /locus_tag="ACHU66_001564"
     CDS             69..425
                     /locus_tag="ACHU66_001564"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR3389833.1"
                     /translation="MVTISACSGSNEIIGTWQAQVADGTELIIEIDKKQIAVYGETSD
                     YVHNGFGFENNMSYYTIEVNEWNIYTIIFPAKDKSIAVLIQPTDFDEPLVGTLIFVMN
                     KNEQPNYLEYGNKYLK"
     gene            630..2660
                     /locus_tag="ACHU66_001565"
     CDS             630..2660
                     /locus_tag="ACHU66_001565"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFR3389834.1"
                     /translation="MLFFKNLKKGMKPSGVVDDQSWSNSRFKLALKVADRSDSMEMLK
                     TPDAAEITQVGRGYLQVGNNEVYELFQSAWSGADYQPDKDEQGIEDHTIYAINDIGQY
                     EILNEDLSGLEQAESIKEVPSELQAIVSKLKALTERLSIQALPQPWLPPLSKEIFLQD
                     LRPQGFKDLWGQASGLVARLGMVDIPSRQSQEVLEHDFEKDGHIALFSSPGMGKSTFV
                     QTVLVDLFRQNNPDRLHTYLLDFGTNGLLALNDYPHVAEYFLVEETEKLQKLIKRLQD
                     EIRERRKLFTDSSVHNIEMYRKLGDRKLADIVIAVDSYDGLKGSEVEVAIDTLLQTLA
                     RDGMGLGIYLILTASRTGALKNNLYSNFKRRIALKMNDDSEARAIVGRTALVIEDVPG
                     RGLIQVDDPELIQLAMPAKGENGLEMIEALRHESQEMAKNWNGALPQGIVIVPEVLDE
                     QTFLQMVETEKAIASDFIPIGLEFEEVHTISLPIPKLKHLLILTEKEQHKLVAVRHLL
                     NMLWHVRKGQSVTLFDVTDEYGRFAGKVKQYYSSSELTENVVEELKMRVLHARKQKEQ
                     LNEFVIISDLPAFMTVDGMSDNDFALLYEEGQRVGLHLIVVANKTYMSLSSGIQRLIK
                     QKLDTVLIAMKMSNQSVVARSEVGREAELAIDEVYLHYQDQQIKLKITKEIE"
     gene            2671..3126
                     /locus_tag="ACHU66_001566"
     CDS             2671..3126
                     /locus_tag="ACHU66_001566"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389835.1"
                     /translation="MQKEKMTVEEFLQLKKDAQVSLILFIVFGLVLFSSMFYITVIGA
                     RTQMYNSLVTMVFLSVLMSAVGPYVFSKNILEKKQPELKQYSTEGYSVSSAFLKRVFL
                     VYSVAIIVVVFGFASAYATRVETILPDDTLPSLEQKSIIEQKLEGNSNE"
     gene            3200..3505
                     /locus_tag="ACHU66_001567"
     CDS             3200..3505
                     /locus_tag="ACHU66_001567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389836.1"
                     /translation="MSHNLHAQRSLSGLQSYIEHCQKVIDRIDSQDGYGYLANFRDKL
                     ADDIKVLKDYRNFVKDSNASFVDLYQTLNAKIGNLNASIANYKSMYNDGKPVWEWVW"
     gene            3514..3846
                     /locus_tag="ACHU66_001568"
     CDS             3514..3846
                     /locus_tag="ACHU66_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389837.1"
                     /translation="MDTNINSIDSQYTYDTIVYDDGQIDGVIQQLNQIIDCLNQISSE
                     IVGLEAQDSSWSGKSKAAYVDLKEFLKSYRKDYQHSIKELKSTVSGLETLLNSIPSAT
                     VLKEIDGA"
     gene            3843..4499
                     /locus_tag="ACHU66_001569"
     CDS             3843..4499
                     /locus_tag="ACHU66_001569"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389838.1"
                     /translation="MIFSIEALYTLHLLNDHSDDLSFLPLPNGQNLDRKSLLKETLEK
                     GFEDLKELQLVVDNQLTERGVEYGYYLEEYHSSPYHCRVDLSYFCAPAVDEFKRYSIL
                     IKEVDEGVFVIERLASVWFLGFLLETHPILHHLDDKMKDYLMYDWTPKSHFSLLVEHG
                     RSEGLRLAVEQLGTIYSDTLFLEIEESIYEYDLTRELMRSIDSETLQSRLIKDLKVEV
                     "
     gene            4502..4936
                     /locus_tag="ACHU66_001570"
     CDS             4502..4936
                     /locus_tag="ACHU66_001570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389839.1"
                     /translation="MSEQIQISLSSQEQIILHALRITELATDITQTIQQVVETIPNFS
                     SQGSFHTIYTTGKNDGFYRYVLKAQELKTLSEVLYRHVETTHQKMVDMDRALAVHITN
                     QFLNSPSTSSEDKQFIREHPEEAVKYIQSEMKKSAPSSGGGS"
     gene            4933..7002
                     /locus_tag="ACHU66_001571"
     CDS             4933..7002
                     /locus_tag="ACHU66_001571"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389840.1"
                     /translation="MTRFYTNRSDFEQNHWLDSQKSRVVAMLRDIDSVSSTVQSEGLS
                     HLYRSRGEASLGASKPSSTFSQAVDRVDSMLRSAGKIHKDIVQNIDSKFTKGLDKSFQ
                     SLNEVNGSKNPYMSKGKYEKTYTTTNYTSNYGYMTQTHTRQVHYSLSDILDGTKSPIP
                     AAQSLYQDRVKNLREQLRQTDQLTDKQIKELESKSDAELVQEFYPTLESDYSQIKNST
                     WQEENKEALGLVDTGLKILAGIALAAGTVMTGGAALPLALAGGAYLFADGAHSAITGN
                     TLITGTQLSTEERIWAGAEAVVTLATFGSFVAGQGALRAGKQIPTTVQTIGKLAGYAD
                     EGVELGQLASGILSGDGTNATLNYIGGKAFEGIVESSVKGLQSQLSHIEVSSPSQIQL
                     NARQGQNFVDLQTVEFGKIASKVENNITIKMTVDGVEQKIRVDAIGFDEAGNIRIQEY
                     TTAQNGLKISRQNLLEDLSKYGGTIVGAGKGDFVGGVEIPKGTRIDVVSQKTGNVTIG
                     SLDTVYSVKDFKAEMEQLPHINLSKKVEDGLVSTNNGSRTSPHHVYSVEEIATHLAMF
                     DDGAVKIMSFESYDYGLQNYGATIGHPDGHYVMPKFVYEKAVRASNGNPRVLEGLLGL
                     EQGYLGNNPVVIDIQRLKNLRMPSGNEPGAWQGLWEPGGYTQGGIPEAVTDQLSESDY
                     TIDEVFK"
     gene            7018..7878
                     /locus_tag="ACHU66_001572"
     CDS             7018..7878
                     /locus_tag="ACHU66_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6572 domain-containing protein"
                     /protein_id="HFR3389841.1"
                     /translation="MKVLRGTDFIIYELNGQYSINWYRGRERFSYPIDEDLVKKALLT
                     SKDGKEVMFYVEHGRWPEEDELEHYNESNVITHRGNNFIVYEENGKYEMHFMTGGFQD
                     REVIYPISKELMERAFESPQDAYEVKIYLMTGHWPDKSQEELDRNFLRQFPEQILKNP
                     TIPKTLFSEYEFTKLLKQAIETVLQTSKIDSLGIVEEHLELLLLDPIKWQEETEIIHL
                     QLLQEKLNNYIHFIESKQYVESYGDDFTEKVINLTFQYAPSDNGLAFLMQVQKVLQPT
                     DIRLKVVVPE"
     gene            8204..8821
                     /locus_tag="ACHU66_001573"
     CDS             8204..8821
                     /locus_tag="ACHU66_001573"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389842.1"
                     /translation="MDAIGFDEAGNIRIQEYTTAQNGLKISRQNLLEDLSKYGGTIVG
                     AGKGDFVGGVEIPKGTRIDVVSQKTGNFSIDSTPNYIQVGRYTTELSKIDLPLEEKVI
                     RLQEFYSDLSDKTDINVPSDPQYVVAVRDGWVEYDWPKNLGYQEGTVQSITRDSGLPD
                     QWDRFGHMGGGNFSDIPSDGPYTYSQRAIPYVENPNAYHKGTFIR"
     gene            8818..9099
                     /locus_tag="ACHU66_001574"
     CDS             8818..9099
                     /locus_tag="ACHU66_001574"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389843.1"
                     /translation="MSYTDFIKLYQESLKVGVQLIIGAQKSSLLKTDLSIKYIKENLV
                     TAIVAQRLYDQSIVQHKMTSREETLKVDEVYLYHDQDYQKVKISKQVVE"
     gene            9099..>9662
                     /locus_tag="ACHU66_001575"
     CDS             9099..>9662
                     /locus_tag="ACHU66_001575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389844.1"
                     /translation="MNRIKKLMMCILGGVTIMGLTGCNKEVEIRTELAPMYEVLDQQS
                     IENFDIFSIEDNLRIYGMESAKAFKTDLAISPDGQFEGMSYDLSVSETEGYPTTYIDG
                     NLKINTTSEVLSDRKMMFEEFHFSEDYFNSLELVKVLDHPNTYLKEITYQEKTPSEYT
                     KMLIDLYDLTTPRMANIEISKVRADEKT"
BASE COUNT         2976 a         1651 c         2328 g         2707 t
ORIGIN      
        1 ttagaaaact tatttaatta aggaaaagtt atgaaaaatt agtcactatt attttatttt
       61 tcatagggat ggtgactata tctgcttgta gtggttctaa tgaaatcatc ggaacatggc
      121 aagcacaggt agcagatggg acagagctga taattgaaat tgacaaaaaa cagattgcgg
      181 tatatggtga gacctcggac tatgttcaca atggatttgg ttttgaaaat aacatgagtt
      241 attatactat agaggttaat gagtggaaca tctatacgat tatttttcct gcaaaagata
      301 aatcaattgc cgtcttgatt cagcctacag actttgacga accgttggtt ggaaccttaa
      361 tttttgtcat gaataaaaat gaacagccaa attatttgga atatggcaat aaatatctta
      421 aatagtcaaa cttatatatt accaatcata gattatttct atccagacaa gattaccttg
      481 tctggatttt ttggtataat ggaaaagtag aaatattctt catttgtgca taaacggtta
      541 ggctaggctt ggtctaaagc ggagctggag gatgagatgg aagtagaatc gctttatttt
      601 atttctattt cgaaacgatg tgagcagcta tgctattttt caaaaacctc aagaaaggta
      661 tgaagccatc tggtgtggtg gatgaccaga gctggtccaa ctcccgcttc aagctggcgc
      721 tcaaggtggc agatcggagc gactccatgg agatgcttaa gacgccagat gcagctgaga
      781 ttacccaggt gggtcgtggc tacctccaag tcggaaacaa tgaagtgtat gaactcttcc
      841 agtctgcctg gtctggggct gattaccagc cagataagga tgagcagggg attgaggacc
      901 ataccatcta tgccatcaat gacattggtc aatatgagat tctcaacgaa gacctgtcag
      961 gtctggaaca ggctgaatcc atcaaggagg ttccgagtga gcttcaagcc atcgtaagta
     1021 agctgaaagc cttgacagag cgcctgtcca tccaggccct gccgcaacca tggttgcctc
     1081 cgctgtctaa ggaaatcttc ctgcaagatc tacgaccaca aggtttcaag gacttgtggg
     1141 gacaggcaag tggtttggtt gcacgcttgg ggatggtgga tataccgagt cgccagagcc
     1201 aagaagtctt ggaacatgac tttgaaaagg atggacacat cgccctcttc tctagtccag
     1261 gtatggggaa atcgaccttt gttcaaacgg tattggttga tttattccga caaaacaatc
     1321 cagatcgtct tcacacttat ctgctcgatt ttgggaccaa tggattattg gctctaaatg
     1381 attatccaca tgttgcggag tatttcttgg ttgaagagac tgaaaaatta cagaagttga
     1441 taaaacgact gcaagatgaa atacgagaaa gaaggaaatt attcaccgat tcctccgttc
     1501 ataatatcga aatgtatcgc aagttaggag ataggaagtt agctgatatt gttattgcgg
     1561 tggacagcta tgatggtctt aaaggttcgg aagtcgaagt ggcgatcgat accttgcttc
     1621 aaactcttgc aagagatgga atggggcttg ggatctattt gatcctaacc gctagtcgaa
     1681 caggggcact caaaaacaat ttgtatagta attttaaacg aagaatagcc ctgaaaatga
     1741 atgatgatag tgaagcgcga gcaatcgttg gaagaactgc tttggttatt gaagatgtgc
     1801 cgggtagagg gcttattcaa gtggatgatc ctgagttgat tcagttagca atgccggcta
     1861 aaggtgagaa tggtctcgag atgatcgaag cattacgtca tgaatcccaa gaaatggcta
     1921 agaattggaa tggagcatta cctcaaggca ttgtcattgt tccagaggtt cttgatgaac
     1981 agactttttt acaaatggtg gagacagaga aagcaatcgc ttcagatttt attccgattg
     2041 gtttagaatt tgaagaggta cacacaatct cgttgccgat tcctaagtta aaacatctat
     2101 tgattttgac tgaaaaggaa cagcataagt tagtagcagt tcgccatctt ttaaatatgc
     2161 tttggcatgt tcgaaagggg cagtctgtga cgctgtttga tgtgacagat gaatatggcc
     2221 gatttgctgg gaaagtgaag caatattatt cttcttctga actaactgaa aatgtagtgg
     2281 aagagttgaa aatgagagtt ttacatgctc gtaaacaaaa agaacaattg aatgaatttg
     2341 tcatcatcag tgatttacca gcctttatga cagttgatgg catgtcagat aatgattttg
     2401 cacttctgta tgaagaggga cagcgtgttg gtttgcactt gattgttgta gctaacaaga
     2461 cttatatgtc gctgtcgagt ggtattcaac gcttgattaa acaaaaattg gatacggtac
     2521 tgatcgcgat gaagatgagc aaccagtcgg tggtggcacg aagcgaggtt ggacgggagg
     2581 cagagttggc catcgatgag gtatacctcc actatcaaga ccaacaaatc aagttgaaaa
     2641 ttaccaaaga aatagaatag gagagttagg atgcaaaaag aaaaaatgac agtagaagag
     2701 tttttacaac tgaagaagga tgctcaggta tccttgattc tatttattgt ttttgggctt
     2761 gtcttgtttt caagtatgtt ttatataaca gtgattggtg caagaacaca aatgtacaat
     2821 tcacttgtga cgatggtgtt tctatctgta ttgatgagtg cagtcggacc ctatgttttt
     2881 tctaaaaata tcttagaaaa aaagcaacct gagctgaagc agtattcgac agagggctac
     2941 tcagtttcta gtgcctttct aaaaagggtt tttctagttt attcggttgc gattattgtg
     3001 gttgttttcg gttttgcctc tgcctacgca actcgggttg agaccatcct tcccgatgat
     3061 accttaccaa gtctggaaca gaagtctatt atcgagcaaa aattggaggg caacagtaat
     3121 gagtgacgag tatttgctta gttgtattac caatagtcgt gaaaagcttg caaagtataa
     3181 gcgagtaagg aatactatta tgagtcataa tcttcatgct caacgtagcc tatctggtct
     3241 ccaatcttac attgagcatt gtcagaaggt cattgatagg atcgatagtc aggatgggta
     3301 cggttatctg gcaaactttc gtgataagtt agcggatgac ataaaggttt tgaaggacta
     3361 tcggaatttc gtgaaggatt caaatgcatc ctttgttgac ttataccaga cattgaatgc
     3421 aaagatagga aatttgaatg cttcaatcgc aaactataaa agtatgtaca atgatgggaa
     3481 acccgtgtgg gaatgggttt ggtaggaggt gctatggata caaatattaa tagtattgac
     3541 agccaatata cgtatgatac aattgtgtac gacgatggtc agatcgatgg tgttattcaa
     3601 cagttgaatc agattattga ttgcctgaat cagatttctt cagagattgt agggctggaa
     3661 gcacaggata gtagctggtc tggaaaaagc aaagctgcct atgttgattt aaaagagttt
     3721 ctcaagtcct ataggaaaga ttatcagcac agtatcaaag agctcaagtc aacggtttcg
     3781 ggattggaaa cccttttgaa ctccatccct agtgcaacag tattgaagga gattgatggc
     3841 gcatgatatt ttcaattgaa gcattgtaca ccctccatct tttgaacgat cattcggatg
     3901 acttaagttt tcttccgctt ccaaatgggc aaaacttaga ccgcaagtcc ttgttaaagg
     3961 aaacgctaga aaaaggattt gaggatttga aagagttaca attggtggtg gataatcagt
     4021 tgactgagcg aggggtggag tatggctact atctggaaga gtaccattcg tccccttacc
     4081 actgcagagt ggacttatcc tatttctgtg ctccggctgt tgatgagttt aagcgatatt
     4141 ctatacttat caaagaggtt gatgaggggg tgtttgtcat tgagcgtctt gccagtgttt
     4201 ggtttttggg gttcttgctg gaaactcatc cgattcttca tcatttggat gataagatga
     4261 aggattattt gatgtacgat tggacaccga aatcccattt tagtcttctg gttgaacatg
     4321 gtcggtcaga gggacttcgt ttggcggtgg agcaactagg gacaatttac agcgatacac
     4381 tctttcttga aattgaagag agtatctatg agtacgattt aacacgagaa ctcatgcgct
     4441 cgattgatag tgaaacactg caatcaaggc ttatcaagga tttgaaagtt gaggtataag
     4501 aatgtcagaa cagattcaaa tttcgctcag ctctcaggaa caaatcattt tacatgcttt
     4561 gcgcattaca gagttggcga cagatattac gcaaactatt cagcaagtgg ttgaaacaat
     4621 tcctaatttt tccagccaag gttccttcca tacgatttat acaactggga aaaatgatgg
     4681 tttctatcgc tatgtgttga aagcccaaga acttaagaca ctttcagaag ttctctatcg
     4741 tcatgtagaa acgactcatc agaaaatggt ggacatggat agagccttgg ctgtgcatat
     4801 caccaaccag ttcttaaaca gcccttcaac gagttctgaa gataagcagt ttatccgaga
     4861 acatccagaa gaggcggtga agtatatcca gagtgagatg aagaaatcag caccatcatc
     4921 aggaggtgga tcatgacacg tttttatacg aatcgctcag attttgagca gaaccactgg
     4981 ctagattctc aaaagagtcg agtcgtcgct atgttgcggg atatcgattc ggtctcttca
     5041 acagtgcagt cggaaggtct ttctcatctc tatcgctctc gtggtgaggc tagtttaggt
     5101 gcaagcaagc cgagttctac ttttagccag gctgttgacc gtgtcgatag catgttaaga
     5161 agcgctggaa agattcacaa ggatattgtc caaaatattg atagtaagtt taccaagggc
     5221 ttagacaaga gttttcaaag cttgaacgaa gttaatggca gtaagaatcc ttatatgtct
     5281 aaggggaagt atgagaaaac ttatacgact actaactata ctagtaatta tggctatatg
     5341 acccaaacgc ataccagaca ggtgcattac agtctttcag acatccttga tggcacgaaa
     5401 agtccaattc cagcagccca gtcattatat caagaccgag tgaagaacct tcgagagcag
     5461 ctccgccaga ccgaccaact aacggataaa caaatcaaag agttggagtc caagagtgat
     5521 gcggagttgg ttcaggagtt ctatccgact ctggagtcag actatagtca aatcaaaaat
     5581 agtacttggc aggaggagaa caaggaagct ttaggcttgg ttgatacagg tctcaaaatt
     5641 ttggcgggca ttgccctagc agcaggaacg gttatgactg gaggtgctgc ccttccgctt
     5701 gcacttgcag gtggcgcgta cctctttgcg gatggagctc actctgcgat tacaggcaac
     5761 accttgatta ctggtactca gttgagcacg gaagagcgta tctgggctgg agctgaggct
     5821 gttgtgacat tggctacttt tggtagcttt gtggcaggtc aaggtgcctt gcgagcaggt
     5881 aaacaaatcc cgacaaccgt tcagaccatt ggtaaattgg ctggctatgc ggatgagggt
     5941 gtggaactgg gacaattggc atctggtatc cttagtggag acggtacgaa tgcgaccttg
     6001 aattacatcg gaggtaaagc atttgaaggc attgtagaga gctctgttaa gggactccaa
     6061 agccaacttt ctcacattga agtttcaagc ccaagccaaa ttcagctcaa cgctcgtcag
     6121 ggtcagaact ttgtggactt gcaaacggtt gagtttggta aaatcgccag caaggtcgag
     6181 aataacatca cgatcaagat gacggttgat ggcgttgagc agaagatacg agttgatgcg
     6241 attggttttg acgaggcagg aaatatccgc atccaggaat acacaactgc ccagaatggt
     6301 ttgaaaatca gccgtcagaa cctcttggaa gacctgtcca agtacggtgg caccattgtc
     6361 ggcgcaggca agggcgactt tgtcggcggt gttgagatac ccaagggcac acggattgat
     6421 gtcgtgtcgc agaagacggg gaatgtgacg ataggatctc tggataccgt ttattctgta
     6481 aaagacttta aagctgaaat ggagcaattg cctcatataa atctgtccaa aaaggttgaa
     6541 gatggcttgg ttagtacgaa taacgggagc cgtacgtcgc ctcaccatgt ttattcagta
     6601 gaagaaattg caactcatct tgctatgttc gatgatggag cagtgaaaat tatgagcttt
     6661 gaatcatatg attatgggtt gcaaaattat ggtgcgacga taggccatcc tgacggtcat
     6721 tacgtaatgc ctaaatttgt ttatgaaaaa gctgtaaggg catctaatgg taatccaaga
     6781 gttttagagg gactattggg attagaacag gggtacctag gaaataatcc agttgttata
     6841 gatattcagc gcttaaaaaa cctccggatg ccttctggta acgagcctgg agcttggcaa
     6901 ggtttgtggg aacctggagg ctacactcag ggggggattc ctgaggcagt gactgatcag
     6961 cttagtgagt cggattatac aatagacgaa gtattcaaat agaaaggtca gatatatatg
     7021 aaagtgttac gagggactga ctttataata tatgaattaa atgggcaata tagcattaat
     7081 tggtatcgtg ggcgagaacg attctcttat ccaatagatg aagatttagt aaaaaaagct
     7141 ctgttaacat ctaaagacgg gaaagaggtc atgttttatg tagagcatgg gcgctggcca
     7201 gaagaggatg agttagagca ttataatgaa tctaacgtaa ttacacatag agggaataat
     7261 tttattgtct atgaggaaaa tggtaaatat gagatgcact ttatgactgg cggatttcaa
     7321 gaccgagagg tcatctatcc tatttctaaa gaacttatgg aaagagcttt tgagtcgcct
     7381 caagatgcct atgaagtaaa aatttactta atgacaggcc attggccaga taagagtcaa
     7441 gaagaattgg atagaaattt cttaaggcag tttccagagc aaattcttaa gaacccgact
     7501 attccaaaga ctctcttttc agagtatgag tttacgaaat tattgaaaca agcaattgaa
     7561 acagtattac agactagtaa aattgatagc ttaggaatag ttgaagaaca tttggaactc
     7621 ttgcttctag acccaatcaa gtggcaggaa gaaacagaaa ttattcattt gcagttattg
     7681 caagaaaagt tgaacaacta cattcacttt atcgaaagca agcagtatgt ggaaagttac
     7741 ggggatgact ttacagaaaa agttatcaat cttactttcc agtatgctcc gtcagacaat
     7801 ggtcttgctt tccttatgca agttcaaaaa gttctacagc cgacagatat tcggttgaag
     7861 gttgtagtgc cagagtagag ttttaagtcc agacagggtc accttgtctg gattttttgg
     7921 tataatggaa gggtagaaat atttttcatt tgtgcataaa cggttaggct agacttggtc
     7981 taaagcggag cctaggagtg ggaatgagat tggagttgtt atctctcgca tccattttga
     8041 aacaagttga gccgctctgc tattaccaca aattcagctc aacgctcgtc agggtcagga
     8101 ctttgtggac ttgcaaacgg tcgagtttgg taaaatcgct agcaaggtcg agaataacat
     8161 cacgatcaag atggcggttg atggcgttga gcagaaaatc tgagtggatg cgattggctt
     8221 tgacgaggca ggcaacatcc gcattcagga atacacgact gctcagaatg gtttgaaaat
     8281 cagtcgtcag aacctcttgg aagacctgtc caagtatggc ggcaccattg tcggcgcagg
     8341 caagggcgac tttgttggcg gtgttgagat acctaagggc acacggattg atgtcgtgtc
     8401 gcaaaaaacg gggaattttt caatagatag cactcctaac tatatccaag taggacgtta
     8461 tactacagaa ctttctaaga tagacctgcc acttgaggaa aaagttataa gacttcaaga
     8521 gttttattca gatctttcgg ataagacaga tattaacgtt ccatcggatc ctcaatatgt
     8581 agtggcggta cgcgacggtt gggttgagta tgattggcca aagaatttgg gttatcaaga
     8641 aggtaccgtt cagtcaatta caagagattc aggattgcca gatcaatggg atcgttttgg
     8701 acatatggga ggagggaact tctcggacat ccctagtgat ggtccctata cctatagtca
     8761 gcgtgccatt ccgtatgtag aaaatccgaa tgcttatcat aaaggaacct ttataagatg
     8821 agttatacag actttataaa actctaccaa gaatcactca aagtaggggt ccaactgata
     8881 attggtgcac aaaaatcatc gttactcaag acagatttat ctattaagta tattaaagag
     8941 aatctggtga cggcaatcgt tgctcaaaga ctttatgatc aaagtattgt acaacataaa
     9001 atgacaagtc gtgaggagac tttgaaagta gatgaagtgt atctatatca tgaccaagac
     9061 tatcaaaaag taaaaattag taagcaggtg gtggagtaat gaatcgaata aaaaaactaa
     9121 tgatgtgtat tttaggaggt gttacaataa tgggtttgac aggttgtaat aaagaagtag
     9181 agattagaac agagttggca ccgatgtatg aggtgttgga ccagcaatct atagagaatt
     9241 ttgatatttt ttcgattgaa gataatttga ggatatatgg tatggagagt gccaaggcat
     9301 ttaagactga cttggctatt agcccagatg gacaatttga aggaatgagt tatgatttgt
     9361 cagtttctga aacggaaggc tatccaacga cttatataga tggaaatttg aaaattaata
     9421 cgactagtga agtgttatcc gatagaaaaa tgatgtttga agaatttcat ttttcagaag
     9481 actatttcaa tagtttagag ttagtaaaag tactggacca ccccaatact tatctgaaag
     9541 agattaccta ccaagagaag actccttcgg aatacacaaa gatgctgata gatttatatg
     9601 atttaactac cccaagaatg gcaaatattg aaattagtaa agtaagggct gacgaaaaaa
     9661 ca
//
LOCUS       DAZQCJ010000074         9644 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000074 DAZQCJ010000000
VERSION     DAZQCJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.075"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            286..753
                     /locus_tag="ACHU66_001576"
     CDS             286..753
                     /locus_tag="ACHU66_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HFR3389845.1"
                     /translation="MSTNRLAWDEYFAAQALLIANRATCKRAKVGAVLVKDNKVIATG
                     YNGSVSGTEHCLDQECLMIDGHCARTLHAEVNAILQGAERGIPKGFTAYVTHFPCLNC
                     SKQLLQVGCKRVVYINEYRMDDYAQYLYKEKGCELVHLPLEVVKQAIADTEFI"
     gene            1476..2666
                     /gene="metK"
                     /locus_tag="ACHU66_001577"
     CDS             1476..2666
                     /gene="metK"
                     /locus_tag="ACHU66_001577"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR3389846.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPDAHVAAE
                     TAVYTGSVHVFGEISTTAYVDINRVVRDTIAEIGYTKGEYGFSAESVGVHPSLVEQSP
                     DIARGVNEALETRQDASQDPLDLIGAGDQGLMFGFAVDETPELMPLPISLSHKLVRRL
                     AELRKSGEIAYLRPDAKSQVTVEYDEDNQPVRVDTVVISTQHDPEVSQEQIRQDVIER
                     VIKEIIPAHYLDDQTNYFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAHPVSVRIDTFGTSTVA
                     ESKLEAAVRQIFDLRPAGIIQMLDLNRPIYRQTAAYGHMGRTDIDLPWEKLDKVEALK
                     TAVL"
     gene            2697..3311
                     /locus_tag="ACHU66_001578"
     CDS             2697..3311
                     /locus_tag="ACHU66_001578"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFR3389847.1"
                     /translation="MKALIVFAHPRKESFTHALVDRVAEALLENGAEVVIRDLYEIGF
                     DPVLRGEDTIHIEDGKFIRQATVFPADVQVEMDLIAESDLLVYIFPSWWNGMPAIMKG
                     YVDRVFQHGFAYSFESDEPRKLFSTKKAIFFTPTGQPQNADGSLTPIDQAMKSLTSEW
                     LFNSNGAQVIDHIFYGRVPYLTREELELYLVDAREQIQRIFHKL"
     gene            3459..4718
                     /locus_tag="ACHU66_001579"
     CDS             3459..4718
                     /locus_tag="ACHU66_001579"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR3389848.1"
                     /translation="MRKIVINGGKTLKGSVTVSGAKNSVVALIPAIILADGIVTLDGV
                     PAISDVDSLVDIMETMGATVKRDGETLEIDPRGVKDMPMPFGKINSLRASYYFYGSLL
                     GRFGQAVVGLPGGCDLGPRPIDLHLKAFAAMGAETTYEGDYMRLATNGQRIQGAHIFM
                     DVVSVGATINTILAAVKAEGRTVIENAAREPEIIDVATLLNNMGARIRGAGTDIITIE
                     GVDSLKGTRHQVIPDRIEAGTYIALAAAVGEGIRIDNVLYEHLESYIAKLEEMGVRMT
                     ISEDSIFVEKQEKLKAVSVKTSPYPGFATDLQQPITPLLLKAEGTGTIVDTIYEKRVG
                     HVQELANLGAKISTRNNHIAFEGPNQLTGTSVKATDLRAGAAMVIAGLMAQGRTEITN
                     IEFILRGYSNIIDKLTELGADIQLIED"
     gene            4813..5385
                     /locus_tag="ACHU66_001580"
     CDS             4813..5385
                     /locus_tag="ACHU66_001580"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR3389849.1"
                     /translation="MTLWTRLAKFAYFETDRLYLRPFSFEDGESFYEIVANPENLPFI
                     FPALEDRNIAFSTMVEKFMRSPLGNWALVDKYSERMIGALCFEKVDERQLSAELSYFL
                     KKDYWRRGLMTEAVRTLVYLAFYEFGLRELVIVTHEENVASQMVAKKAGLVQVAQYRG
                     SDRYSHKTRNYLKFSLKRAEFSLEMYEENE"
     gene            5382..6242
                     /locus_tag="ACHU66_001581"
     CDS             5382..6242
                     /locus_tag="ACHU66_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HFR3389850.1"
                     /translation="MITIKSQREIDAMNRAGDVLAGIHIGLREIIKPGVDMWEVEEYV
                     RKTCKEKNVLPLQIGVDGELMDYPYATCCGLNDEVAHAFPRHYKLKEGDLLKVDMVLS
                     EPLDKAVVDVSKLNFNDVKAMKKITQTYRGGCADSCWAYAIGQVSEEVQNLMDVTKEC
                     LYRGIEQAVVGNRIGDIGAAIQEYAEGLGYGVVRDLVGHGVGPTFHEEPMVPHYGTKG
                     RGLRLREGMVLTIEPMINTGTWEIDTDMKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            6290..7219
                     /locus_tag="ACHU66_001582"
     CDS             6290..7219
                     /locus_tag="ACHU66_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFR3389851.1"
                     /translation="MKKTKIVEGLKTFCSTFFSFYRSADSDVTSVAVAYYLLISIFPI
                     LLTLANLLPYYPFDVDMILSVVAEFVPDKLYPSVSSFITSVLTKPSSSWLGISILTTL
                     WTLSRSMTILQKAFNKAYGINEHRDFIIGHLIGIFLGIGLQVIILLSITLLTFGQAVF
                     SYINKLVPIEDAWLKGLLSQTQLVGLMALFAALVMLYFFLPNVRIKKVRYVFPGTLFV
                     LLTMTSIGKLFSIYVDNYANKLLDFRIVTAVVFLVFMLWFIFMAQVLIIGAMINATVQ
                     SMQVEEFHARDGDIVSILNRLKARFTAIDKEWE"
     gene            complement(7379..7816)
                     /locus_tag="ACHU66_001583"
     CDS             complement(7379..7816)
                     /locus_tag="ACHU66_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFR3389852.1"
                     /translation="MKKLFYQLINNEVILYLIAGVAATLVYMVTRMGLFLVIPSILLV
                     TLLANAVAILFAFWTNDRFVFKQAREGWPQRLVKFVSARIATLVMDMALAYLLVQAYP
                     QLIGQFVNHDLTLVNAISTLFSQVLVIVLNYVLSKLFVFKDKK"
     regulatory      7845..7947
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            7945..8460
                     /locus_tag="ACHU66_001584"
     CDS             7945..8460
                     /locus_tag="ACHU66_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFR3389853.1"
                     /translation="MNTSKWTARDMAEIALVAAIYVALTLTPPLNAISFGAVQFRLSE
                     MLNFLAFYNRKYIIAVTIGCMISNLIGFGVIDLFVGGFSTLIFVTLGVILFEKYKKDY
                     LFGGLFNKAFFYFSFFFAVTMFTIALELKVLYDLPFFLTWLTTAAGELLSLLVGAVVI
                     DKLSKKINFEK"
     gene            8576..9418
                     /locus_tag="ACHU66_001585"
     CDS             8576..9418
                     /locus_tag="ACHU66_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR3389854.1"
                     /translation="MTRYTFSNGVTVPKIGFGTWQIPDGEVAYGAVSYALEAGYRHID
                     TAQIYGNEASVGRAIADSGLAREDIFLTTKVWNDKIGYEDTLASVEESMQKLQVDYLD
                     LLLIHWPNPKASRETIGWKKRNAQVWKALEELYRAGKVKVIGVSNFMEHHLEALLETA
                     EIIPMANQIMLAPGTPQSELVAYCKAKGILLEAYSPFGTGSLFQSQEAADLAKEAGCS
                     VAQLALAWSLDKGFLPLPKSTSPENIKANLEIDGLAISPTAVAKLDKLEGVKGRLDPD
                     QAEF"
BASE COUNT         2737 a         1673 c         2254 g         2980 t
ORIGIN      
        1 tggaaggagg gatacaatta tttattatcc tttgacaagt agggttttgc ttgagctatt
       61 gcctcagaat gacaattgta aacatttcga caatttcata atggaaattg gaaatgaaaa
      121 attaaagttt attaaatcat tgaatacctt acagttactt ggttcacata gtcaagtgtt
      181 tatgcatcca aggtattcat tcaatgaatc aactttttat catttttaaa agcacaaata
      241 gttggtggtg agcagtgatt tataaaaata tataaaggag aaatcatgtc aacaaatcgt
      301 ttagcttggg atgagtattt tgcggctcag gcacttttga ttgccaatcg ggcgacctgt
      361 aagagagcca aggtcggtgc ggttttggtt aaggacaata aggttatcgc aacaggctac
      421 aatggttctg tttcaggaac cgaacattgt ttggatcagg aatgccttat gattgatggt
      481 cattgcgcta gaaccttgca tgcggaagtc aatgccattt tacaaggagc ggagcgggga
      541 attccaaaag gttttactgc ctatgtgacc catttcccat gtctcaactg ctccaagcaa
      601 ttgttacagg ttggttgtaa gcgggttgtt tatatcaatg aataccgcat ggatgactat
      661 gctcagtact tgtataaaga aaagggctgt gagttggttc atttgcctct agaggtggtt
      721 aaacaggcaa ttgcagatac cgaatttatc taatgatttt gtagaagagt gggagtgaga
      781 aagaactcga ccattcatag aagagttcgt caacaatttt tcgttttaag ttgttgagct
      841 gaaacagtct atcctcagac tgtttcactc ccacccccgc atagctccaa cagtcagagt
      901 agtgactgtt ggaggttgga aatgaagcga actctgttcg catcagtcgt aatggtcaga
      961 tttggagtgt aaaacacgaa ctgctgagat ttgcttcgca aatcttatct ccaaccttta
     1021 acagtccact agactgttaa acccaatcaa ccactgcgct gagatgttga cacgaactct
     1081 gagtagtggt cctgggcttt ttgcccagcc tcctctttat ttttaagaaa atgctaggat
     1141 agtcggtcaa tctatgctat actagaaacg ttattaagtc ccgaaaaggt agtttataga
     1201 ctagttaata tttgcagaaa tacttgaaac acaattaaag aagctggtaa cattgaatag
     1261 taagcgtaaa aactttacta cacttcagtc accattttct tcaaataagg ctgatttcat
     1321 acagtctggt attgtctttc tcgttgagta tctggaaatt tccctttgct taagacaggt
     1381 gtctttcgca aagaactagt cttcgactag ttctttgtaa ccttagtaac ccgctaggtt
     1441 tctagcatat attctatttt aagggggaca tttttatgtc agaacgtaag ttgtttacgt
     1501 ctgaatctgt ttcggagggt catccggata agattgcaga ccagatttca gatgccattt
     1561 tggacgctat tttagcagag gatccagatg cgcacgtagc ggcagagacg gctgtttata
     1621 caggtagtgt tcatgtgttt ggagagattt caactactgc ctatgtggat attaaccgtg
     1681 tggtacggga taccattgcg gagattggct atacaaaagg tgagtatggt ttttcagctg
     1741 agtcggttgg ggttcatccg tcacttgtag agcaatcgcc agatattgcc cgaggcgtca
     1801 atgaagcttt agaaactcgt caagatgcta gtcaagatcc attggatttg attggtgcag
     1861 gtgaccaggg gctcatgttt ggttttgcag tagatgaaac gcctgaactt atgccgttgc
     1921 caatttcact tagtcacaaa ttggtgcgtc gtttggccga gttgagaaaa tctggtgaaa
     1981 ttgcttatct ccgtccagat gctaagtcac aggtaacagt tgaatacgat gaagacaatc
     2041 aacctgttcg tgtggataca gtcgttattt cgacccagca tgatccagaa gttagccagg
     2101 agcaaattcg tcaggatgtc attgaacgtg tgatcaagga aatcattcca gctcattatc
     2161 tggatgacca aaccaactac tttatcaacc caactggtcg ctttgtcatt ggcggacctc
     2221 aaggggactc tggtttgaca ggccgtaaga ttatcgttga tacctatggt ggttattctc
     2281 gtcatggcgg cggtgccttc tctggtaagg atgcgaccaa agtggaccgt tctgcatctt
     2341 atgcggcacg ctacatcgca aaaaatatcg tcgcagctgg tttggctaaa aaggcggaag
     2401 ttcaattggc ttacgctatc ggtgttgctc acccagtttc agttcgcatc gatacctttg
     2461 gtacgagcac agtagcggaa agcaaactag aagcagctgt ccgtcagatt tttgacctgc
     2521 ggccagcagg tattatccag atgctggacc tcaatcgccc gatttacaga caaactgcgg
     2581 cctatggcca catgggccgg accgacattg acctaccttg ggaaaaatta gacaaggtag
     2641 aggcgttgaa aactgcggtt ttgtaatgaa atagttgtcc atttagtgag aagtttatga
     2701 aagcgttaat tgtctttgca caccctagaa aagaaagctt tacacatgct ttggttgata
     2761 gagtggcgga agctctgctg gaaaatggag cagaagttgt catccgagat ttatacgaaa
     2821 taggattcga tcccgtgtta cgtggagaag ataccattca tattgaagat ggcaaattta
     2881 tacgtcaggc aacggttttt ccagcagatg ttcaagttga aatggatttg attgccgaaa
     2941 gcgacttact agtctacata tttccgagtt ggtggaatgg aatgcctgct attatgaaag
     3001 ggtacgtcga ccgtgtattt caacatggct ttgcttacag ttttgaatct gatgaaccac
     3061 gtaaattatt ttccactaag aaagctattt tctttacgcc aacagggcag ccacaaaatg
     3121 cagatggaag tctgacaccg attgatcaag cgatgaaaag cttgacctca gaatggctct
     3181 tcaatagcaa cggtgctcaa gtcattgacc atatcttcta tgggcgagta ccttatttaa
     3241 cgagagaaga gctggagttg tatctcgtgg atgcaaggga gcaaattcag agaatttttc
     3301 ataaattatg aagagagatt cactaatagt atgtaaatat gaaggactga gctcattggc
     3361 tcggtccttt tgcatgctat ataactagct ttttccaaga ctttatgata gaatgaaacg
     3421 tatgcctttt taggcaaatt aagaaagtga atgttagtat gagaaaaatt gtaattaatg
     3481 gtggaaaaac actgaaaggg tcggttaccg ttagtggtgc aaaaaactca gttgttgctc
     3541 ttattccggc cattattctt gcagacggga ttgttactct ggacggagta cctgcaatct
     3601 cggatgtaga tagcttagtt gatattatgg aaacaatggg cgcgacggtc aagcgtgatg
     3661 gtgaaacctt ggagattgac cctcgtggtg taaaagacat gcctatgcca tttggcaaaa
     3721 ttaatagctt gcgtgcatcc tactattttt acggttccct ccttggtcgc tttggtcaag
     3781 ctgttgttgg cttgccaggt ggttgtgatt tgggaccgcg tccgattgat ttgcacttga
     3841 aagcattcgc ggcaatggga gctgagacaa cttatgaagg tgactatatg cgcttggcaa
     3901 ctaatggtca gcgtattcaa ggggcccata tcttcatgga tgttgtcagc gtcggtgcga
     3961 caattaatac tatcttagca gctgtcaaag cagaaggccg tacagttatt gaaaatgcgg
     4021 cgcgtgagcc tgaaattatt gatgtggcaa cgttgcttaa taatatgggg gcacgtatcc
     4081 gtggtgcggg tacagatatt atcaccattg aaggcgtaga cagtctaaaa ggaacacgtc
     4141 atcaagtgat tccagaccgt attgaagcag ggacttatat tgcccttgct gctgctgttg
     4201 gtgaaggtat tcgtatcgat aatgtccttt atgaacactt agagagttac atcgctaagt
     4261 tggaagaaat gggtgttcgc atgaccattt ctgaagatag tatttttgtt gaaaagcaag
     4321 agaaattgaa ggcggtaagt gtgaaaactt caccttatcc aggttttgcg acagacttgc
     4381 agcaacccat tacaccttta ttgcttaagg cagaagggac tggtacgatt gttgatacga
     4441 tttatgaaaa acgtgttggt catgtgcagg agcttgctaa tcttggagca aaaatctcta
     4501 ctcgtaacaa ccatattgcc tttgaaggtc caaatcaact gactggtaca tctgttaaag
     4561 ctaccgattt gcgtgcggga gccgcaatgg ttattgctgg tttaatggct caagggcgta
     4621 cagaaattac aaatattgag tttattctac gtggttactc aaatattatt gataaactga
     4681 cagaattggg tgcagatatt cagttgattg aggattagag agtctaattg agaactactt
     4741 tctcaaaacg taaacgattt aaaaacaaaa aaatctgggg ctgggagtaa ttctcagccc
     4801 cttgggtaca ctatgacact ttggacaaga ctagcgaagt ttgcttactt tgaaacggac
     4861 cgtttgtatt tacgaccatt ttcttttgaa gatggagaaa gtttctatga aattgtagca
     4921 aatcccgaaa atcttccttt tatctttcca gctttggagg acagaaatat tgctttttcg
     4981 acaatggttg aaaaatttat gcgttcacca ttgggaaact gggccttagt tgataagtat
     5041 tcggagagga tgattggtgc gctttgcttt gaaaaggtag atgaacgtca actttctgcg
     5101 gagctcagct atttcctaaa aaaagactac tggagaagag gcttaatgac ggaagcagtg
     5161 aggactttag tctatctagc tttttatgag tttggtctga gggagctggt tattgtcact
     5221 catgaggaaa atgtggccag tcagatggtg gcgaaaaaag ctggtttggt acaggttgcg
     5281 cagtatcgtg gaagtgaccg ctatagccat aaaacaagaa attatctaaa atttagcttg
     5341 aagagggctg aatttagttt agaaatgtat gaggagaatg aatgattacc attaaatcac
     5401 aacgagaaat tgatgcaatg aaccgtgctg gagatgttct ggctggtatt catattggtt
     5461 tgcgagagat tatcaagcca ggtgtggaca tgtgggaagt agaggaatat gtccgtaaaa
     5521 cgtgtaagga aaagaatgtt ttgccactcc aaattggtgt tgatggagaa ttgatggatt
     5581 atccttatgc gacttgttgt ggattgaatg acgaggtcgc tcatgccttt ccgcgtcatt
     5641 acaaacttaa agaaggtgat ttactgaagg tcgatatggt tttgagcgag cctttggata
     5701 aagcggtagt tgatgtttct aagctcaact ttaatgatgt caaggctatg aagaaaatca
     5761 cccagaccta tcgtggtggt tgtgcggatt cctgctgggc ttatgcgatt ggtcaggtct
     5821 ctgaggaagt tcaaaacttg atggacgtga ctaaggaatg tttgtaccgc ggtatcgagc
     5881 aagcagttgt tggcaatcgt atcggcgata tcggtgcagc cattcaagaa tatgcagaag
     5941 gtttgggcta cggtgttgtt cgtgacttgg tgggtcacgg cgttggacca accttccacg
     6001 aagagccgat ggttcctcat tatggaacta aaggtcgtgg tcttcgcttg cgtgagggca
     6061 tggttttgac tattgagcca atgattaata cagggacttg ggaaattgat acggatatga
     6121 agactggttg ggctcataag acacttgatg gtggcttgtc ttgtcaatac gaacaccaat
     6181 ttgtcatcac caaagatgga ccggttattt tgaccagtca aggtgaagaa gggacctatt
     6241 aattctcggt gtggtcgtga ataatagatg aatgaggaag gaggagagaa tgaagaaaac
     6301 caaaatagtc gaaggtttga aaacattctg ctcaaccttc ttttcttttt atcgttctgc
     6361 tgatagcgat gtaacaagcg ttgcagtagc ttactattta ttaatttcta ttttccctat
     6421 tcttctaact cttgcgaatt tactgccata ttatccattt gatgtggata tgattttatc
     6481 ggttgtagct gaatttgtgc cagacaagct ctatccttcg gtttcatctt ttatcacttc
     6541 tgtattaaca aagccgtcgt catcgtggtt agggatttca attctgacaa ctctttggac
     6601 cctgtcacgt agtatgacta ttttgcagaa ggcctttaat aaggcttatg gtatcaatga
     6661 acaccgtgat tttattattg ggcacttgat tggcatattt ttaggaattg gtctgcaggt
     6721 aattatcctc ttaagtatta cgcttttgac tttcgggcag gcagtttttt cttatattaa
     6781 taagctggtt cctattgaag acgcctggct gaagggactt cttagtcaaa cacagttagt
     6841 aggtttaatg gctctgtttg ccgccttggt tatgctttat ttctttttgc caaatgttcg
     6901 gataaaaaaa gttcgatatg tttttccagg aacgttgttt gttctgttga cgatgacttc
     6961 tattggaaag ttattttcga tttatgttga taattatgct aataagttgc tggattttag
     7021 aatagtaaca gctgtcgtct ttcttgtttt tatgctttgg ttcattttta tggctcaggt
     7081 actcatcatt ggtgcaatga ttaatgctac ggttcaaagt atgcaggtag aagagtttca
     7141 tgccagagat ggggatattg tttcaatctt gaataggcta aaggcaagat ttacagcgat
     7201 agataaagaa tgggagtgag aaagaactct attttcaaaa atgagttcgt cttctcaccc
     7261 ccgcacagtt gattaggcca gatttggagt gtgaaacgcg aacaaatctg ccaatcaacc
     7321 actgcgctga gatgttgaca cgaactctga gaagcgaggt tggtcttttt gcccaacctt
     7381 atttcttatc tttaaataca aataatttac ttaaaacgta gtttaggaca atgaccaaga
     7441 cttgagagaa gagagtggaa atggcgttga caagtgtcaa atcatggttg acaaactgcc
     7501 caatcaattg tgggtaggct tgtactaata aataggctag agccatatcc atgaccaagg
     7561 tcgcgatgcg ggcagataca aacttgacca atcgctgagg ccaaccctct ctggcttgtt
     7621 tgaaaacgaa acggtcgttg gtccaaaagg caaagaggat agctactgca ttggctaaaa
     7681 gagtaaccaa tagaatggaa ggaatgacta aaaatagccc cattcgagtg accatataga
     7741 caagggttgc tgccacacct gcaatcaaat agagaataac ttcgttattg atgagttgat
     7801 aaaatagttt tttcatagga ctagtctatc atttttagca aaaatatgct atactaatca
     7861 acgttgttga caaattcaat gacccttggg acttgctcac tttacccaag catattttaa
     7921 gcttttttgc taaaggagaa ttttatgaat acatctaaat ggactgccag agatatggca
     7981 gaaattgctc ttgttgcggc gatttatgtc gctcttacct tgacaccgcc attgaatgct
     8041 atttccttcg gggctgttca gtttcgtttg tctgaaatgc tcaatttctt ggctttttat
     8101 aatcgtaagt atattatcgc tgtgaccatt ggctgcatga tttccaactt gattggtttt
     8161 ggtgtgattg acctctttgt gggtggattc tcgacattaa ttttcgttac gttgggagtt
     8221 atcctgtttg aaaaatacaa gaaggactat ctattcggtg gattatttaa caaggctttc
     8281 ttttatttct cattcttttt tgcggtaact atgtttacca ttgctttaga attaaaagtc
     8341 ttatatgatt taccattctt tctaacttgg ttgacaacag cagcaggaga attgctttct
     8401 ctattagttg gagctgttgt tatcgataaa ctatccaaga aaataaattt tgaaaagtaa
     8461 gtatacaata aatgtgagca gacactttcg ggtgtctttt atttttaggt cgcattattt
     8521 tgtattgatt gacaatttag tttatactgg ggatagaaga aataggaggt cttttatgac
     8581 aagatatacg ttttcaaatg gagtaactgt tcctaaaatt ggttttggaa cttggcagat
     8641 acccgatggt gaagtggcgt acggtgcggt ttcttatgca ttggaagctg gttatcgtca
     8701 tattgatact gctcaaattt atggcaatga agctagtgtt ggtcgggcta tagctgatag
     8761 tggcctagct cgtgaggaca tttttttaac aactaaggtt tggaatgata agattggcta
     8821 tgaagatacc cttgcttcag tagaagaatc catgcaaaaa cttcaggtgg attatctgga
     8881 tttgttactg atccattggc caaatccaaa ggctagtcgt gagacaattg gatggaaaaa
     8941 acgcaatgcc caggtttgga aggcattaga agagctctat cgtgctggaa aagtgaaagt
     9001 aattggagtt tctaacttta tggaacatca cctagaagct ttgctggaaa cggctgagat
     9061 tatcccgatg gctaatcaga ttatgttagc tccaggaact cctcagtcag aattagttgc
     9121 ttactgcaaa gccaaaggga ttctcttgga ggcctatagc ccatttggta caggaagcct
     9181 tttccaaagc caagaggcag ccgatttggc taaagaagct ggttgtagtg ttgctcagct
     9241 ggcactagct tggtctttag ataaaggctt tctccctctc ccgaaatcaa ctagtccaga
     9301 aaatattaag gcgaatttgg aaattgatgg cttggctatt agtccgacag cagttgcaaa
     9361 gttggataaa ctagaaggag taaaaggtcg acttgacccg gatcaggctg aattttaaaa
     9421 aatatgaata taaggttgga tatgtccagc cttatttttg tttctagact aattataatg
     9481 gaagtagtat gtatttcgaa atatatatta gatattattt tatgtaaaaa agtagatttt
     9541 tctcgcggca tcgtgttttt tgcgtaataa acatcacatc gtttcaactt aaatctgtat
     9601 tttgggtaaa gacaaaaaat attatcacaa caaaatatat caca
//
LOCUS       DAZQCJ010000075         9626 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000075 DAZQCJ010000000
VERSION     DAZQCJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.076"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            65..2089
                     /locus_tag="ACHU66_001586"
     CDS             65..2089
                     /locus_tag="ACHU66_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFR3389855.1"
                     /translation="MKKNIRLKSSILALVAGFSVIATQAVLADELAVQIMGVNDFHGA
                     LDMTGTARLEGETVRNAGTAALLDAYMDDSQAEFEETAAETETPAESIRVQAGDMVGA
                     SPSNSGLLQDEPTVKVFNKMDVEYGTLGNHEFDEGLDEYNRIMTGEAPKKGQFNEIVD
                     NYTREAAKQEIVIANVIDKETGEIPYGWKPYAIKTIPVNDKEAKIGFIGVVTTEIPNL
                     VLKKNYEQYTFLNEAETIAKYARELAEKGVNAIVVLAHVPATSKDGVAAGEAADMIAK
                     LNEIYPEHSVDLVFAGHNHVYTNGTTGKTLIVQATSQGKAYADVRAVYDTDIADFKAV
                     PTAKIIAVAPGQKTPSPEIQAIVDEANTIVKKVTEQKIATASQATDISREVNEFKESA
                     VGNLVTSAQLAIARKSGYDVDFAMTNDGGIRADLKVQEDGTVTWGAAQAVQPFGNILQ
                     VVQMTGEQIYTALNQQYDEGEKYFLQMSGIKYIYTKADNPTEENPYKVVKAFKEDGTE
                     IVPTETYTLVINDFLFGGGDGFSIFKEAKLIGAINPDTEVFVEYLTDLEKAGQTISAT
                     IPGRKAFVEKYVEEPKAEEKEDNAGTTTDVKTPEKANDGGDSVTNQKATEQPAPSGGM
                     APISNKKTEKASGNQTLPNTGQEALGSLLISLGGLVSLGMAVSVRRKEGE"
     gene            2287..2358
                     /locus_tag="ACHU66_001587"
     tRNA            2287..2358
                     /locus_tag="ACHU66_001587"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2320..2322,aa:Arg,seq:cct)
     gene            complement(2396..3490)
                     /locus_tag="ACHU66_001588"
     CDS             complement(2396..3490)
                     /locus_tag="ACHU66_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR3389856.1"
                     /translation="MAYFRKRDNGWEYRISYKAPDGSYKQKSKSGYRTKAEAVQAASQ
                     AEIELSNGIVEDKNITLAEYFEKWMLVHKKPHVGPETFGKYEYTLKLITIYFHETKLS
                     KINATSYQNIINELAKCYVKDSVKRFNSHIRAAIKVAIHQGILKKDFTEIVKIFSDVE
                     SKKEEDKYLELDEYEQVITDYRKTIKYQSHFFLYTIGKTGLRFSEAAGITEPIVDREN
                     MCLRIRRTYKVYGKKKGWGPTKNPQSERDVPFDSEWLKAYDEYMKVGYIDNPDKRLFT
                     KLTGTGENKILKKKTRQTFNVHGLRHTYVSWLIYHDVDVVTIAKLVGHKDATETLKTY
                     SHLFKAKQEESFDKVRNLMEKFGADLGRES"
     gene            complement(3616..4050)
                     /locus_tag="ACHU66_001589"
     CDS             complement(3616..4050)
                     /locus_tag="ACHU66_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR3389857.1"
                     /translation="MTFQQGEVYLVNFTQKGGNEFYGKHYAIILTPPDKTDGTLLAVP
                     LTGKKAGKKYRGGITLDNTKYQDTPSKPKAYAYVRKIQEIDKRKIIYKTKKQVDSSGV
                     PLTDKSGKALFQKIYKPAYQLDQTDLDKLKAKIKEVLQLDIE"
     gene            complement(4225..4980)
                     /locus_tag="ACHU66_001590"
     CDS             complement(4225..4980)
                     /locus_tag="ACHU66_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR3389858.1"
                     /translation="MPIENKDIFSKNLKYYMDKKGVDRNQLCSDLDLKYTTVRDWIKG
                     ITYPRIGKIELLANYFGINKSDLIEEKSTIPSTTPKTVSDDVLRLDRDLHSNNHKSWI
                     RYGNALLDKQNTVTDSKNTVNELQATYHTYNYYDQPASAGTGQYLNDVKVETIELPIE
                     VDADFVVPIYGDSMEPEYHSGDYIFVKLSVDLSDGDIGVFAYNGDAYIKQLRITDQGA
                     YLHSLNPDYDNIPITADTDFRTIGEVVEVYRER"
     gene            5153..5287
                     /locus_tag="ACHU66_001591"
     CDS             5153..5287
                     /locus_tag="ACHU66_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389859.1"
                     /translation="MVDILKSLADNDLAIPIIILGLVREARLWHKQVLEHKRNLQNKK
                     "
     gene            5421..5741
                     /locus_tag="ACHU66_001592"
     CDS             5421..5741
                     /locus_tag="ACHU66_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFR3389860.1"
                     /translation="MKVERMGEKAKAEIKVCGITYLVIVQEHFKAYDDERNLWGYCDY
                     ERQIIYVRESLSEERKRQVLIHELTHAMLQEAGYKEQDEDLVTRFSIILHQVLIDNPK
                     LFNV"
     gene            complement(5731..5859)
                     /locus_tag="ACHU66_001593"
     CDS             complement(5731..5859)
                     /locus_tag="ACHU66_001593"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389861.1"
                     /translation="MAKTCRPSKPVSKAGKTLATSKSASAKSKAGKTLANHKEAKH"
     gene            6002..6193
                     /locus_tag="ACHU66_001594"
     CDS             6002..6193
                     /locus_tag="ACHU66_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR3389862.1"
                     /translation="MWEKIEKLLKEKSMTKNKLATLAGINKNSLIDLKMGRKKSLKFE
                     DVIKIADALDVSLDEFRKG"
     gene            6218..6403
                     /locus_tag="ACHU66_001595"
     CDS             6218..6403
                     /locus_tag="ACHU66_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR3389863.1"
                     /translation="MLRARDNLTQKQAGQLVGVTADTWANWEKGKTNPDVKTAYKIAE
                     NFGLSIDDIIFLDSIAV"
     gene            6439..6660
                     /locus_tag="ACHU66_001596"
     CDS             6439..6660
                     /locus_tag="ACHU66_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFR3389864.1"
                     /translation="MSKQRYGRPSTGQKGNNRPTVVISRENYDEVDSLSIGTGMSRSA
                     IIDYFISEGLKRARIEEVVIKTKRLVLED"
     gene            6674..6805
                     /locus_tag="ACHU66_001597"
     CDS             6674..6805
                     /locus_tag="ACHU66_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389865.1"
                     /translation="MNELERTALNEILRTVAYIAEKVDELDAKISLSDLQVLEHQEN"
     gene            complement(6756..7445)
                     /locus_tag="ACHU66_001598"
     CDS             complement(6756..7445)
                     /locus_tag="ACHU66_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFR3389866.1"
                     /translation="MIFQAKINSSVSRPVTIDDICPNCKKPTNPHLVNSSYFPLGEEK
                     TSLVLTFRCLGCKHFWTEEFIATRHRINSYTDKYEIEHLKVTPSLPSDIPISDDVELV
                     SPIGKQIYVQALKAEHEQLDHIAGIGYRKALEFFVKDFSIVTNPDDEDKIIKMPLKQV
                     IEKYIKDDDLKTFALASAYIGNDEGHYYRNNPDKDFSHLKNYLHGVIHYMEMKLNFLD
                     AQELVNRSKKS"
     gene            7499..8209
                     /locus_tag="ACHU66_001599"
     CDS             7499..8209
                     /locus_tag="ACHU66_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFR3389867.1"
                     /translation="MNEIINVSVNDNQEPVVSGRQLHEALGVKTAYKDWFPRMTEYGF
                     VEGQDFCSNLSESTGGRRAVDHIIKLDMAKEIAMIQRTDRGKQVRQYFIQIEKDFNSP
                     EKIMARALLLADKKVHQLEAQIEADKPKVLFADAVSASHSSILVGDLAKLISQNGFKI
                     GANRLFARLRENGYLIKRKGSDWNMPTQKSMELGLFEIKETTITHADGHISISKTVKV
                     TGKGQQYFINKFLADDVA"
     gene            8564..8821
                     /locus_tag="ACHU66_001600"
     CDS             8564..8821
                     /locus_tag="ACHU66_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389868.1"
                     /translation="MTEAILTLGIFAVPILAVAIAEQRKAEKERKCEEFEEIRRRDYL
                     YGFKAGMGYQSTCDIEKARNGLKRDAQQVDKEIARYAEMVG"
     gene            8802..9026
                     /locus_tag="ACHU66_001601"
     CDS             8802..9026
                     /locus_tag="ACHU66_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389869.1"
                     /translation="MQKWLANFLKQEKPAIPRPLYTLEQENQILHDMVREIAEQRNEY
                     RIENQRLRDENERLRRILEYDEEYDEHDSD"
     gene            9079..9444
                     /locus_tag="ACHU66_001602"
     CDS             9079..9444
                     /locus_tag="ACHU66_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR3389870.1"
                     /translation="MQHIARNTHENYSKINNHPAQNSELSLQAKGLLFVLMSNKDTWR
                     PYIDQLSKRSKNGREAHRNAFEELKDSGYIRIYRKSLGRGRGIQNYPLVSDIPITDSY
                     WEYWKEKVDDELSTGESSE"
BASE COUNT         3003 a         1651 c         2149 g         2823 t
ORIGIN      
        1 cgtaagaaaa ggattattat agatatgtca atatgaaaaa aatagaagga gcattgtttt
       61 gcttatgaaa aagaatattc ggttgaaaag cagtatacta gctcttgtag ctggttttag
      121 tgttattgca acacaagctg ttttggcaga tgaattagct gtccaaatta tgggagttaa
      181 tgatttccat ggtgcgcttg atatgacggg gacagcgcga ttggaagggg aaacagttcg
      241 taatgcagga actgccgctt tacttgatgc ttacatggat gattcacaag cagaatttga
      301 agaaacagca gcagaaacag agacacctgc agagtctatc cgtgttcaag ctggggatat
      361 ggttggtgca agtccatcga attctggact tttgcaagat gaaccaactg taaaagtctt
      421 taataaaatg gatgttgaat acgggacttt ggggaaccat gagtttgatg agggacttga
      481 tgagtataac cgtatcatga ctggtgaagc tccaaaaaaa ggtcagttta atgagattgt
      541 agataattat actcgtgaag ctgctaaaca ggagattgtt attgctaacg ttattgacaa
      601 agaaacgggt gaaattccgt atggttggaa gccctacgct attaagacta ttcccgtgaa
      661 tgataaggaa gctaagattg gctttattgg tgtagttacg acagaaattc ctaatcttgt
      721 tttgaagaaa aactatgagc agtacacttt tttgaatgag gcagagacga ttgctaaata
      781 tgcgcgtgag ttagctgaaa aaggtgtaaa tgcgatagtt gtactggctc acgttccagc
      841 tacaagcaag gatggtgtgg ctgctggtga agcagcagat atgattgcta agctaaatga
      901 aatctatcct gaacactcag ttgaccttgt atttgctggt cataaccatg tctatacaaa
      961 cggtacaacg ggcaaaacct tgattgttca agctacctca caaggtaagg cttacgcaga
     1021 tgttagggct gtttatgata cagatattgc cgactttaaa gctgttccga ctgcgaaaat
     1081 tattgcagta gcaccagggc agaaaacacc aagtccggaa attcaggcaa ttgtagatga
     1141 ggcaaatacc attgttaaaa aagtaacaga gcaaaaaatt gctacagcta gtcaagcgac
     1201 agatatttca cgcgaagtga atgaatttaa agaaagtgct gtaggtaatc tagtaacatc
     1261 ggctcaatta gctatcgcta ggaaatcggg ttatgatgtt gactttgcaa tgacaaacga
     1321 tggcgggatt cgggcagatt tgaaggtcca agaagatgga acagttactt ggggagcagc
     1381 acaggctgtt caaccatttg ggaatatcct acaagtcgtt caaatgaccg gtgagcagat
     1441 ttatacagcc ttaaatcaac aatatgatga aggtgaaaaa tatttccttc aaatgtctgg
     1501 aattaaatat atctacacga aggctgacaa tccaacggaa gaaaatcctt ataaggttgt
     1561 taaagccttc aaagaagatg ggacggagat tgttccgaca gaaacctata cacttgtcat
     1621 caacgacttc ttatttggtg gtggggatgg cttctcgatt ttcaaagaag ctaaactgat
     1681 tggtgctatc aatccagata cagaagtatt tgtggagtac ttgactgatt tagaaaaagc
     1741 aggtcaaacc attagtgcaa caattccagg tagaaaagca tttgtagaga agtacgtaga
     1801 agaaccaaaa gcagaagaaa aagaagataa tgctgggaca actactgatg tgaaaacacc
     1861 tgagaaagca aatgacggtg gcgatagtgt aacaaatcag aaagcaaccg agcaaccggc
     1921 accatctgga ggtatggctc ctatttcaaa taagaaaact gaaaaagcat caggaaatca
     1981 aacacttcca aataccggtc aagaagccct aggctcactt cttattagct tgggtggctt
     2041 agtttcactc ggaatggctg tctcagtgag acgtaaggaa ggggagtagg aatcaagcat
     2101 aaaaacgaca aactttcgaa gtacattcta gaattacttc ttttagagac tatcatgaga
     2161 gaaatgcata aaaataataa gtatttcatc ttagagtaag ttctgctttt ttgtaaaaat
     2221 ttgaaaaaac ttacataacc ttgctcttgc aatctgctct cttctggggt acaatagaga
     2281 agcagacttc ccttagttaa atggatataa caaattcctc ctaagaatta gttgcaggtt
     2341 cgattcctgc aggggagatg aaaatacaac aaaaaccctt gataaacaag ggtttttaac
     2401 tttctcgccc caaatccgcc ccaaattttt ccattaaatt tctgactttg tcgaatgatt
     2461 cttcttgttt tgccttgaat aagtgcgaat atgttttcaa tgtttcggtc gcatccttgt
     2521 gtcctactaa cttggcaatg gtcacaacgt ccacgtcatg atagatcagc cagctaacgt
     2581 aggtatgacg taagccgtgt acattaaatg tttgacgtgt ctttttcttt aaaattttat
     2641 tttcgccagt ccctgtcaat ttggtaaaca atcttttatc tggattgtct atatatccaa
     2701 ctttcatgta ctcgtcgtat gctttcagcc actcactatc aaatggcaca tctcgttctg
     2761 attgcggatt cttagtaggt ccccaacctt tcttctttcc gtaaaccttg taagtcctgc
     2821 ggattcgtaa acacatattt tcgcggtcaa cgataggctc tgtaatgcct gctgcttccg
     2881 agaaacgaag tccggttttt ccgatagtgt acaggaagaa gtgggactgg tacttaattg
     2941 tctttcgata atctgtgatt acttgttcgt attcatcaag ttccaagtac ttatcctcct
     3001 ctttcttgga ttcgacatcg gagaaaatct tgacaatttc ggtaaaatct ttttttaaaa
     3061 tcccttggtg gatagcaact ttaattgctg ctcttatatg cgaattgaac cttttgacac
     3121 tatctttcac ataacatttt gccagttcgt ttataatgtt ttgataggaa gtagcgttta
     3181 ttttcgagag tttcgtttcg tgaaagtata tagtaatcag tttaagggta tattcatatt
     3241 taccaaatgt ttctggtccg acatgaggct tcttgtggac aagcatccat ttttcaaagt
     3301 attcagcaag ggtaatgttc ttatcttcca caatgccgtt ggacagctca atttcagctt
     3361 gggatgctgc ttgaacagcc tctgccttgg ttctatatcc tgacttagat ttctgcttat
     3421 atgagccgtc tggggcttta tatgagatac ggtattccca tccgttatcc ctttttctaa
     3481 aatatgccat tgatttaccc tttctaattt gataaaatgg gtatagtaaa gagacctact
     3541 gcaaagcagg tttttactat acggaattac cctacactca agctttggtc ggcggagagt
     3601 gtggggattt ttttgttatt cgatatctaa ttgcaagact tctttgattt ttgcttttaa
     3661 cttgtccaaa tctgtctggt cgagttggta ggctggtttg tagattttct ggaatagtgc
     3721 ctttcctgat ttgtcagtga ggggaactcc agaggagtct acttgctttt tggtcttgta
     3781 gatgattttg cgtttgtcaa tttcttgtat ttttcggaca taggcgtagg ctttgggttt
     3841 tgatggtgtg tcttggtatt tggtattatc cagcgtgata ccacctcggt actttttacc
     3901 tgctttttta cctgttaatg gtacagccaa gagtgtgccg tctgttttat cgggcggtgt
     3961 cagaatgata gcgtagtgct tgccgtaaaa ttcatttccg cctttttgtg tgaaattgac
     4021 aaggtagact tctccttgtt ggaatgtcat ggtaactcct tgataaaaaa taaggtgcac
     4081 ttagaaaagt acaccagact gtttgtcctt gacgaacaag gctttataaa taaatatcgt
     4141 atccttgacg aacaaggctt ttgactaacc tcattatacc aaaatgagtg aaatagtcaa
     4201 ggatatttat ttctggtgtt gaatttacct ctccctatac acctccacga cttcgccgat
     4261 ggttcggaag tcggtgtctg ctgtgatggg gatgttgtca tagtctgggt tcaggctgtg
     4321 gaggtaggcg ccttggtctg tgatacggag ttgcttgatg taggcgtcgc cgttataagc
     4381 gaatactccg atgtcgccgt cagatagatc tacggatagt ttgacgaata tataatcgcc
     4441 tgagtggtat tctggttcca tggagtctcc gtagataggg acaacgaagt cggcatccac
     4501 ttcaataggt aattcgatag tctcaacttt cacatcattc agatactgac ctgtgccagc
     4561 ggaagcgggt tggtcgtagt agttgtaggt gtggtaggtg gcttgcagct cgtttactgt
     4621 attcttactg tctgttactg tattttgttt atctagaagc gcatttccgt accgtatcca
     4681 gcttttgtgg ttgtttgaat gtaaatccct atccaatctc aaaacgtcgt cagaaacggt
     4741 tttaggggtg gtagaaggga ttgtggattt ttcttcaatc aaatctgatt tgttaattcc
     4801 aaaataattt gcaagtaatt cgattttccc aattcgtggg tatgtaatac cttttatcca
     4861 gtctcgtaca gttgtatact ttaaatctaa atcagaacag agttggtttc tatcaacccc
     4921 ttttttgtcc atatagtatt ttagattttt cgagaaaata tctttgtttt ctatcggcat
     4981 agcatttttc ctcgctttct aatgaagatt ttacggcaaa aacgcaaaaa aatcaagaaa
     5041 aaacaaaaaa attgcgaaaa aaacgcaaaa agttcttgac attgcggttt aaccgcagta
     5101 taatataatc aaggtcaggg aaatgaccga actaaaacag gaggaaaagc aaatggtgga
     5161 catactaaaa agcctagctg ataacgactt agcaatcccg attatcattc taggcttagt
     5221 aagagaagct cgcttatggc acaaacaagt gcttgagcac aagcggaact tacaaaacaa
     5281 aaagtaagag caaggggcga aagccccaac ctcttatttg aagtatacca ccatttgcca
     5341 aagaaagcaa tgatctattg gttagctggt ttgttggtcc tgtcgtttgt gatacggcag
     5401 attgtgaagt ggaggaagaa atgaaggtgg aaaggatggg agagaaggcg aaggcagaaa
     5461 taaaagtttg tggcattact tatttggtaa ttgtacagga acatttcaaa gcatatgatg
     5521 atgaacgaaa tctgtggggg tactgcgatt acgagcggca aataatctat gttcgagaaa
     5581 gtctttcaga ggaaagaaaa aggcaagtgc taatccatga attaacacat gccatgttgc
     5641 aagaagccgg atacaaagaa caagatgaag atttagtaac gcgattttct ataatcttgc
     5701 atcaagtttt aattgataat ccgaagctat ttaatgttta gcttccttat gattggctaa
     5761 ggtcttgcct gctttggatt ttgcagaagc agacttgcta gtcgccaatg ttttgccagc
     5821 tttgctaaca ggtttggatg gtctacaagt tttcgccata ttcttctcct ttctatcaaa
     5881 attttatgaa taaaagatgt tgggttattc atgagaatat tatagcagaa agttattgaa
     5941 aaatcaacat gtagttgtgg ataacttttt gaatactata tctagtaact gaaggaagaa
     6001 tatgtgggaa aaaattgaaa aactgctaaa agaaaaaagt atgactaaaa ataagcttgc
     6061 tacgcttgca ggcatcaata aaaatagcct aattgatcta aaaatgggta gaaagaaatc
     6121 cttgaagttt gaggatgtca ttaaaatcgc tgacgctttg gatgtcagcc tagatgaatt
     6181 tagaaagggg tgaggagtat tgaaatggac gctaaaaatg ttgcgtgcca gggacaatct
     6241 tacgcaaaaa caagctggtc agctggtagg tgttactgct gacacatggg caaattggga
     6301 aaaagggaaa acaaatccag atgtcaaaac agcctataaa attgcagaaa attttggatt
     6361 gtctatagat gacattattt ttttagatag cattgcggtt taaccgcata aaaccagcca
     6421 aactagaaag ggactaacat gtcaaaacaa agatacggtc gcccaagtac agggcagaaa
     6481 gggaataacc gtcccacagt ggtcattagt cgtgagaact acgacgaggt agacagcttg
     6541 tcaattggta caggaatgag ccgtagtgct attattgatt attttatcag tgaaggcttg
     6601 aaacgtgctc gtattgaaga agttgtcatc aagactaagc gtcttgtcct tgaggactag
     6661 aaaggagaag gggatgaacg aactagaaag aacagccctc aatgaaatac tgaggactgt
     6721 ggcctatatt gcggagaagg tggatgaact tgacgctaag atttctttga gcgatttaca
     6781 agttcttgag catcaagaaa attgagtttc atttccatgt agtgaataac tccgtgaagg
     6841 taatttttga gatgagaaaa atctttatca ggattatttc tatagtaatg accttcgtcg
     6901 ttgccaatat aagcagatgc aagtgcaaat gttttaaggt catcatcctt gatatatttt
     6961 tcgataacct gttttaacgg cattttaatg attttatctt cgtcatcagg atttgtgaca
     7021 atagagaaat ctttaacaaa aaactcaagt gcctttcgat agccgattcc tgcgatgtgg
     7081 tcgagttgtt catgttctgc ttttagggct tgaacataga tttgtttacc gattggggaa
     7141 actaattcta catcgtcaga aataggtata tcacttggga ggctaggagt aactttaaga
     7201 tgttcgattt cgtatttatc ggtgtaggaa ttaatccgat gccttgttgc tataaattct
     7261 tctgtccaga agtgcttaca acctaagcat ctaaatgtta agaccaaact tgttttttct
     7321 tcgccgagag gaaaataaga agagttcacc agatgtggat tggttggttt tttacaattt
     7381 ggacagatat catcgatagt tacaggtcta gaaacagaag aatttatttt tgcttgaaat
     7441 atcataatat ttctccaatc gtttttattt taattatatc aaatcagaaa ggaattttat
     7501 gaacgaaatt atcaacgtta gtgtgaatga taatcaagag cctgttgtgt ctggtcggca
     7561 gttgcatgag gctttgggtg tcaagacggc atataaggac tggttcccta gaatgaccga
     7621 atatggattt gtcgaaggac aggacttctg ctcaaatttg agcgaaagta cgggaggtcg
     7681 tcgagcggtt gaccatatta tcaagctgga catggccaag gaaattgcta tgatccaacg
     7741 gacagaccga ggcaagcagg tacggcagta ctttatccaa atagaaaagg acttcaacag
     7801 tccagagaag attatggctc gcgctctgct attggctgac aagaaggtgc atcagctgga
     7861 agcacagatt gaggcggata aacccaaggt gctatttgca gacgctgtga gtgctagtca
     7921 ctcatctatc ttggttggag acctagctaa gttaatcagt caaaacggct ttaaaatcgg
     7981 tgctaatcgc ttgtttgcgc ggttgcgtga gaacggctat ctcattaagc gcaagggcag
     8041 tgattggaac atgccaacgc agaagtctat ggaactaggt ctgtttgaaa tcaaagagac
     8101 gactatcaca catgctgacg gtcatatctc gattagcaaa actgtaaaag ttactggcaa
     8161 aggtcagcag tattttatca ataaattttt ggctgatgat gttgcttgaa aaacaaaaaa
     8221 accactgcgg gaacagtggc ttactaaaaa aatcacttaa attataacac acgaaagtga
     8281 ggtttgacaa gatggatgac attgctgaaa gctacatatc acgatttatc agtcaactaa
     8341 aagtcagact ggtagaagtg tttgaggtgt ttaacataga acttgcgctg cctttgctac
     8401 tcaacagcaa gcaatgcaag aagttgttgg gtattgcaaa tgagacggaa ttccaaaggg
     8461 tgtcacatct gaaggatttc ccaagaattg ataagaaagg gtcgcaccca cgatttccac
     8521 gagatgcggt ggttgagtgg atgcgtgtaa attggaagtt gatatgacag aagcaatttt
     8581 aacattagga attttcgccg tgccaatctt ggcagtggca attgcggaac agcggaaagc
     8641 agaaaaagag cggaagtgtg aagaatttga agaaattcgg cgcagagact acctgtacgg
     8701 ctttaaagcg ggcatggggt atcagagtac ctgtgacatc gaaaaagctc gtaacgggct
     8761 aaagagagac gctcagcaag tggataagga gattgcacgg tatgcagaaa tggttggcta
     8821 attttttaaa acaagaaaaa cctgctattc cacgtccgct ttacacacta gaacaggaaa
     8881 atcaaatatt gcatgacatg gtccgcgaaa tcgctgaaca acggaacgaa taccgtatcg
     8941 aaaatcagcg attaagggat gaaaatgaac ggttgaggag gattttggaa tatgacgaag
     9001 aatatgacga acatgacagc gattgataac acgcttcaac ttgtgattgt tggagtttag
     9061 gaggaatggt tatggcttat gcagcatatt gcaagaaata cacacgaaaa ctactctaaa
     9121 ataaataatc accctgctca aaacagcgaa cttagtttac aagctaaagg gttactattt
     9181 gtgttgatgt cgaacaaaga tacttggcga ccgtacatcg atcaactttc caaacgctca
     9241 aagaacggtc gagaagctca tagaaatgcc tttgaagaac taaaagacag cggctatata
     9301 cgtatctaca gaaaaagtct agggagagga agaggaattc agaactaccc cttagtttcc
     9361 gacatcccta tcacagatag ctattgggag tactggaaag aaaaggttga tgatgagtta
     9421 tccacaggtg aatcgtcaga gtgaatttac aacttacggg ttttacaaag ttgtattttt
     9481 caaaagttga attttacaaa gttgtatttt tcaaaagttg aaaaacccga cactaataat
     9541 aactaaataa aaataataac taacttaata ataatctagg ggctatcgcc cactaataaa
     9601 caataagagg ctaaagcctc taacta
//
LOCUS       DAZQCJ010000076         9538 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000076 DAZQCJ010000000
VERSION     DAZQCJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.077"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..874
                     /locus_tag="ACHU66_001603"
     CDS             <1..874
                     /locus_tag="ACHU66_001603"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR3389871.1"
                     /translation="TVLVLGYFDGIHLGHKALLDRARQVADEKGLSVTVLTFPETPRL
                     AFSRFSPELLLHLTSQDQRYHLLEQYGVDQLVLTDFTSEFASNTPQQFLERYIKGLNA
                     QVLVAGFDYHFGNCRADVTDLTELFDGQVEIVSEVSLGGEKVSSTRIRQAIQSGEVSL
                     ANQLLGYPFMTEGIVVHGDARGRTIGYPTANLAPFDRVHLPSEGVYVADVEVDGKRYR
                     AMTSVGKNVTFDGTEMRIEAHIFGFHRFIYGEKITIFWLEKIRDMVKFDGIEGLMEQM
                     KSDEKFALQWGKYA"
     gene            867..1613
                     /locus_tag="ACHU66_001604"
     CDS             867..1613
                     /locus_tag="ACHU66_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HFR3389872.1"
                     /translation="MLKRLIRHPFYEKAMTYIAVVYVFLIVLELMDASIANSSGYRVL
                     DLLLWFIFVCDYFAQLYYADDILEYVQGHILELIAIIPFDSFFSFLRLGRLARLFRLV
                     KVTRIFALSNRFWDTINKLLHTNSLSKVLMLNISAVLVSSVLLSVIEGKSFFDALWWS
                     IVTMTTVGYGDIVPQDTISKIIAILLMLVGICTFGMVTSTITRFFSETERETKLDILM
                     AKIDEQNETLLRLEKKIESLEKLGELQGRD"
     gene            1688..2089
                     /locus_tag="ACHU66_001605"
     CDS             1688..2089
                     /locus_tag="ACHU66_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFR3389873.1"
                     /translation="MITLFLSPSCTSCRKARAWLTSHEVPFIEHNIMTSPLTAEELKN
                     ILALTENGTDDLISTRSKVFQKLDIDVDELSVKQLIGLIETYPSLLRRPIILDEKRMQ
                     IGFNEDEIRAFLPRQYRKQELKNATLRAEIG"
     gene            2104..2382
                     /locus_tag="ACHU66_001606"
     CDS             2104..2382
                     /locus_tag="ACHU66_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFR3389874.1"
                     /translation="MNKNYSYPLDFSWSTEEISSVLSFLNQVEAAYEKGADAVAILAS
                     YKSFKDVVKSKGQERQIDRDFEAVSGYSTYRVVKAAQDKGKGVIRFGN"
     gene            2372..3130
                     /locus_tag="ACHU66_001607"
     CDS             2372..3130
                     /locus_tag="ACHU66_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFR3389875.1"
                     /translation="METKYHFAQTIVLEAGNFLRQHLHDNLQIEEKSDFTDLVTHLDK
                     QVQEMLTQQIQSRYQSDGILGEEGGDVSSIHEGNVWVIDPIDGTTNFIAQKTDFAIMI
                     AYFENGVGKFGIIYDVMRDKLFHGGDSFPVYCNQERLKKPELRPLRQSLIGVNAKLYA
                     ANAHGVAELANQSLGTRSVGSAGIGFAHVLEGRLFAYASYLCPWDYAAASIIGSSLGL
                     VMLSFDGPSLAYDKREFVMLVSESHLEEVKGYIF"
     gene            3130..4431
                     /locus_tag="ACHU66_001608"
     CDS             3130..4431
                     /locus_tag="ACHU66_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFR3389876.1"
                     /translation="MLLPQDFIDKYNHLLGSEAQAFFESFEQDPISGFRTNPLKESQL
                     DFEQPIPNMTWSYYGKVSGKSPEHVTGLVYSQEPAAQVVGQIAAPEKGMKVLDLAAAP
                     GGKSTHLLSYLENTGLLVSNEISNKRAKILAENIERFGARNVVVTNESADRLAKVFPG
                     YFDMIVLDAPCSGEGMFRKDPDAIQYWSKHYPAQCASLQREILESALNMLAPGGQLIY
                     STCTWSPEENEEVVTWLLEQYDLELIDIPKINGMVEGVGYPEVARMYPHHFAGEGQFV
                     AKFRFLGEVSQKKLKPGKSNLNREQQALWKEFEQKHLRVQLDGLLQVFGDNLYLLPHG
                     LPDLSKVKIARNGLHLGTYKKKRFEPSFALGLALHSSEVENRVEISLEEFSSYTAGHT
                     IAVSKDLANGWYQVAVQGNGLGFAKIVSGTLKNGFPKGLRF"
     gene            4568..5437
                     /locus_tag="ACHU66_001609"
     CDS             4568..5437
                     /locus_tag="ACHU66_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFR3389877.1"
                     /translation="MKKKQKLGIAALFLLSSMALSGCSSWIDRGESITAVGSTALQPL
                     VEAAADEFGSVNIGKSVNVQGGGSGTGLSQVQSGAVQIGNSDVFAEEKDGIDASKLVD
                     HQVAVAGLAVIVNEQVSVKDITTEDLRKIFTGEITNWKELGGKDLEISIINRAASSGS
                     RATFDSVIMDGQTATQSQEQDSNGMVKSIVAQTPGAISYLSFAYLDDSVKTIKLNGFE
                     PTAENVATNDWPIWSYEHMYTMGKATDLTEEFLDYMMSDEVQNGIVESMGYISINDMK
                     VVKSADGTVTEKE"
     gene            5440..6357
                     /gene="pstC"
                     /locus_tag="ACHU66_001610"
     CDS             5440..6357
                     /gene="pstC"
                     /locus_tag="ACHU66_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFR3389878.1"
                     /translation="MKNEQLAKELSSPSKNSRLEKFGKVITFLCLSLIVFIVAMILIF
                     VAQRGLSTFFVDGVSITDFLFGSKWEPSSKIFGALPMISGSFIVTILSAVVATPIAIG
                     AAVFMTEISPKRGAKILQPVIELLVGIPSVVYGFIGLQVVVPFVRSIFGGTGFGILSG
                     VFVLFVMILPTVTFMTVDSLRAVPRHYREASLAMGATRWQTIWRVILNAAKPGIFTAV
                     IFGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSVLTMGIGNTVMGTVQNNVLWS
                     LALVLLLMSLIFNMIMKFITREGKKNYAR"
     gene            6347..7234
                     /gene="pstA"
                     /locus_tag="ACHU66_001611"
     CDS             6347..7234
                     /gene="pstA"
                     /locus_tag="ACHU66_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR3389879.1"
                     /translation="MHAKKFDKLATGILYSIAGVIVVILTSLILYILLRGLPHVSWDF
                     LTSKSSSYKAGGGIGIQLYNSFFLLVVTLLISVPLSTGAGIYLAEYAKKGPLTNFIRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSILSGALALTVFNLPQMTRNVEDSLRHVH
                     HTQREAGLALGLSRWETVLHVVIPEALPGIVTGIVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNPLSVTSPISIFRQSETLAVHIWKVNSEGTIPDATQVSAGSAAILLVFILIF
                     NLSARYIGKKLHAKLTSAA"
     gene            7259..8062
                     /gene="pstB"
                     /locus_tag="ACHU66_001612"
     CDS             7259..8062
                     /gene="pstB"
                     /locus_tag="ACHU66_001612"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR3389880.1"
                     /translation="MADYNWNERHIITFSEENIALSTKDVHVYYGKNEAIKGIDMQFE
                     KNKITALIGPSGCGKSTYLRSLNRMNDTIDIAKVTGQILYEGIDINRPDMNVYEIRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAISVKPQILLMDEPASALDPIATMQLEETMFELKKDYTIIIV
                     THNMQQAARASDYTAFFYLGDLIEYDKTANVFQNAKLKSTSDYVSGHFG"
     gene            8074..8832
                     /gene="pstB"
                     /locus_tag="ACHU66_001613"
     CDS             8074..8832
                     /gene="pstB"
                     /locus_tag="ACHU66_001613"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR3389881.1"
                     /translation="MTSPILQVKDLSVYYNKKKALNNVSIDFYPNEITALIGPSGSGK
                     STLLRSINRMGDLNPEVTLTGAIDYNGKNIYSPRTDTVDLRKEIGMVFQQPNPFPMSI
                     YENVVYGLRINGIKDKQVLDEAVERSLIGASIWNEVKDRLHDSAVGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEDTLYGLKDKYTMVLVTRSMQQASRISDRTG
                     FFLDGDLIEYNLTKEMFLNPANKETEDYITGKFG"
     gene            8861..9517
                     /gene="phoU"
                     /locus_tag="ACHU66_001614"
     CDS             8861..9517
                     /gene="phoU"
                     /locus_tag="ACHU66_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR3389882.1"
                     /translation="MLRAQFEEELGKLHNQFYAMGNEVLAQINNAVRAFTTHDRELAK
                     EVIEADKKINAFEVKLEKKSLEIIALQQPVSTDLRRVITVLKATSDLERMGDHAVSVA
                     KAAIRMKGEVRIPVVEEEIKKMGREVRQLVEETLELYLGDADMERAYEVAARDEVVNE
                     FYSTIQELTTEEIKKNPESLVAGRDYYQVLTYLERVGDYAKNICEWVVYLRTGDITEL
                     "
BASE COUNT         2801 a         1685 c         2149 g         2903 t
ORIGIN      
        1 gactgttttg gtattaggct attttgatgg gattcatttg gggcataagg ccctcttaga
       61 ccgtgctaga caagttgcag atgaaaaagg attgtcagtg accgtactga cttttccaga
      121 gacccctcgt ctggcttttt ctcgctttag cccagaactt ttactgcatt tgaccagtca
      181 agatcagcgt tatcacttgt tggagcaata cggtgtagac cagctggttt tgacagattt
      241 taccagtgag tttgcaagca atactcctca gcagttttta gaacgctaca tcaaggggtt
      301 gaacgcacaa gtcttagtgg caggtttcga ttatcatttc ggaaattgca gagcagatgt
      361 gacagacttg acagagttat ttgatggtca ggtagaaatt gtaagtgaag taagcctggg
      421 cggagagaaa gtttcttcaa ctcgcattcg tcaggctatc cagtcagggg aggtctctct
      481 agctaatcaa cttttaggtt atccatttat gacggaggga attgttgttc atggagatgc
      541 cagagggcgc actattggtt atccaactgc aaatctagct ccttttgacc gtgtccattt
      601 accatctgag ggagtctacg tcgctgatgt cgaagtagat ggaaaacgct atcgagctat
      661 gacaagcgtt gggaaaaatg tgacctttga tggaacggag atgcggatag aagcccatat
      721 ttttgggttt caccgcttta tctacggaga aaaaataact attttttggc ttgaaaaaat
      781 tcgagatatg gtcaaatttg atggaattga aggattgatg gagcagatga aatctgatga
      841 gaaatttgcc ttgcaatggg ggaaatatgc ttaaacgatt gattcgtcat cctttttatg
      901 agaaggctat gacctacata gctgtagtct atgtatttct tatcgtactt gaattaatgg
      961 atgctagtat tgccaattct agtgggtatc gggtactaga cttgctctta tggtttattt
     1021 ttgtttgcga ttattttgct cagctttatt atgcggatga catacttgaa tatgtacagg
     1081 gacatatctt ggaattaatt gccattattc catttgattc attcttcagt tttcttcgct
     1141 tgggaagact tgcacgactc ttcagattgg tcaaagtaac gagaatattt gccctgtcta
     1201 atcgattctg ggatacaatc aacaagcttt tgcataccaa cagtttatcc aaagtgttga
     1261 tgttaaatat ctcagcagtg ctagtttcaa gtgtcttatt atcagtaatt gaagggaaat
     1321 cattcttcga tgcactttgg tggtccattg taacgatgac aacagtaggg tatggagata
     1381 ttgtccccca agatacgatt tctaaaatta tagccatcct cttgatgttg gttggtattt
     1441 gtacttttgg tatggttacc tccacaataa ctcgtttttt ctctgaaaca gagcgagaaa
     1501 cgaagttgga tatcttgatg gcaaaaattg atgaacaaaa cgaaacatta ttacgcttag
     1561 agaaaaaaat agaatcttta gaaaaattag gtgaattaca gggaagagat tagatttact
     1621 attgaaaact cttttaagtt agagtataat aataagaaga aaaatgtttt tagaggggat
     1681 ggaagacatg attactttat ttttgtcacc gagttgtacc agttgtcgta aagctcgtgc
     1741 ttggctgaca agtcacgaag tcccttttat tgagcacaat attatgacta gccctctaac
     1801 tgctgaggaa ttaaagaaca ttttagcatt gacagaaaat gggacagatg acttgatttc
     1861 tacccgttca aaagtttttc agaaactaga tattgatgtt gatgagcttt ctgtgaagca
     1921 gcttattggg ttaatcgaaa cctatccgag tctcttacgg cgtccgatta ttttggatga
     1981 gaagcggatg cagattggtt ttaacgaaga tgaaattcgt gcatttcttc cgcggcagta
     2041 tcgcaaacaa gagctaaaaa atgctacatt gagagcagaa ataggataag aaagataaaa
     2101 gatatgaaca aaaattattc atatccactt gatttttcgt ggagcacaga ggagatttcc
     2161 tccgtgcttt cttttttgaa tcaggtggag gcagcttatg aaaaaggtgc agatgcggta
     2221 gctatattag ctagttataa aagctttaaa gatgttgtta aaagtaaggg acaggaacgt
     2281 caaattgatc gtgactttga agcagtttct ggctattcaa cctatcgagt tgttaaggcg
     2341 gctcaagata aggggaaagg agttattcgt tttggaaact aagtatcact ttgcccaaac
     2401 gattgtcctg gaagctggaa attttctccg acagcatcta catgataatc ttcagattga
     2461 agaaaagagt gactttacag acttggtaac gcaccttgat aaacaagtgc aagaaatgct
     2521 gactcaacag attcaatctc gctatcaaag tgatgggatt ttaggtgagg aaggtggaga
     2581 cgtttcttct attcatgagg gaaatgtttg ggttattgat ccaattgatg gcaccacaaa
     2641 ttttattgct cagaaaactg attttgccat tatgattgcc tattttgaaa atggagtagg
     2701 aaagttcggt atcatttatg atgtcatgcg agacaaactt ttccatggag gagattcatt
     2761 ccctgtttat tgcaatcaag aacgcttaaa gaaaccagag ctgcgccctc ttcgccagag
     2821 tttgataggt gtaaatgcaa agttgtatgc agccaatgca catggagtgg cagaattagc
     2881 aaatcagagt ttaggtacaa gatctgtagg aagtgctgga attggtttcg cccacgttct
     2941 ggaaggaaga ttatttgcct atgcttctta tctgtgtcca tgggactacg cagcagccag
     3001 tattattggt tcctctttag gcttagtcat gttaagtttt gatggtccga gtctggccta
     3061 tgataagcga gagtttgtca tgctagtatc agaatcccat cttgaggaag tgaaggggta
     3121 tatattctaa tgctattacc acaggatttt atagataaat ataatcattt gcttggtagt
     3181 gaagcccaag cattttttga gagtttcgaa caagacccaa tctcaggatt tcgcacaaat
     3241 cctcttaaag aaagccaatt ggattttgag caaccaatcc ctaatatgac ttggtcatac
     3301 tatggtaagg tctcagggaa atctccagaa catgtaactg gcttagtcta ctcacaagag
     3361 ccagctgcac aggttgtcgg tcagattgca gctccggaaa aagggatgaa agtgttggac
     3421 ttggcagcag cgccaggagg aaagtctact cacttgcttt catacttaga aaatacaggg
     3481 cttttggtta gtaatgaaat ttccaacaaa cgagcaaaaa tattggctga aaatattgag
     3541 cgtttcgggg cgagaaatgt agtcgtgacc aatgaatctg ccgatcgttt ggccaaggtc
     3601 tttccaggat atttcgatat gattgtccta gacgctccct gttcaggtga agggatgttt
     3661 cgaaaagacc ccgatgcgat tcaatattgg tccaaacatt atccagcaca atgtgcaagc
     3721 ttacagcgtg aaattttaga atcggccttg aatatgttgg ctccaggtgg tcaattgatt
     3781 tattctactt gcacttggtc accagaggaa aatgaagaag tagtgacgtg gctcttagaa
     3841 cagtatgatt tggaactaat agacattcct aaaatcaatg gaatggtaga aggagtcggt
     3901 tatccagaag ttgctcgtat gtatccccac cattttgcag gagaaggtca gttcgttgcc
     3961 aaatttagat ttctaggaga agtcagtcag aaaaaattaa aaccaggaaa atccaatttg
     4021 aatagagaac agcaagctct ctggaaagaa tttgaacaga agcatttaag ggttcaattg
     4081 gatggtttac tgcaggtttt tggcgacaat ctctacctac tgccacacgg ccttcctgat
     4141 ttgtcaaaag taaagattgc tcgaaatggt cttcacttgg ggacgtataa gaaaaaaaga
     4201 tttgaaccaa gttttgcttt aggtctagca ctccatagtt cggaggttga aaatcgagtg
     4261 gaaatcagct tagaagaatt ctcgtcctat actgcaggac ataccatagc tgtgtccaag
     4321 gatttagcta atggctggta tcaggtagca gtccagggga acggtcttgg tttcgcaaaa
     4381 attgtatctg gaacgcttaa aaacggcttt ccaaaagggt tgagatttta aaataaatca
     4441 gcaaattttt ttgcaacttg tctttttata tgatatagtg gatgagtgga aatatcttgt
     4501 aaattctatg taaaatacag agttaatttt aggagattcc actgaaaaat gtaaaggaaa
     4561 aaacgaaatg aaaaaaaagc agaagcttgg aattgcagct ttgtttcttt tgagtagtat
     4621 ggctctttct ggttgttctt cttggatcga tcgaggagag tctattacag ccgtaggttc
     4681 gacggcgctt caaccacttg ttgaggcagc agcagatgaa tttggtagtg tcaatatcgg
     4741 taaatccgtc aacgttcagg gtggtggatc aggtactggc ttgtcacaag ttcagtctgg
     4801 agctgttcag attggtaact ctgacgtatt tgctgaggaa aaagatggta ttgatgcaag
     4861 taaacttgtt gaccatcaag tagcagtagc tggtttggcc gttattgtaa atgagcaggt
     4921 atcggttaaa gatattacta cagaagatct gcgtaaaatt tttactggtg agattaccaa
     4981 ctggaaagaa ctaggtggta aggatttgga aatttcaatc attaaccgtg cagctagttc
     5041 aggttcacgt gcgacctttg atagtgttat catggatgga caaaccgcaa ctcagagtca
     5101 agaacaagat tctaacggta tggtaaaatc tattgttgct cagacaccag gtgcaatttc
     5161 ctacctatca tttgcttatc tagatgattc cgttaaaaca attaagttaa acggttttga
     5221 accaactgct gaaaatgtag caacaaatga ttggccaatt tggtcctatg agcatatgta
     5281 tacgatggga aaagcgactg atttgacaga agaattttta gattacatga tgtcagatga
     5341 agttcaaaat ggtatcgttg aaagtatggg atacatttct atcaacgata tgaaagttgt
     5401 gaaaagtgct gatggcacgg tgactgagaa ggagtaagca tgaaaaacga acaactagca
     5461 aaagagttat cgtctccatc taaaaattct cgattagaga agtttggtaa ggtaattaca
     5521 ttcctttgtc tatcattgat tgtctttatt gttgcaatga ttttgatttt cgttgcgcag
     5581 cgtggattat cgactttctt tgttgacggt gtgagcatca ctgatttctt atttggaagc
     5641 aagtgggaac caagctcgaa aatatttggt gccctgccaa tgatttcagg ttctttcatt
     5701 gttacgattc tgtcagctgt tgttgcgacc cctatcgcaa tcggtgcagc agtctttatg
     5761 acagaaatat caccaaaacg tggtgcaaaa atcttacaac ctgttatcga acttttggtt
     5821 ggtattccat cggttgtata tggtttcatt ggtttgcaag tagttgtacc atttgtacgt
     5881 tcaatcttcg gtggaacagg tttcggtatc ttgtcgggtg tatttgtact tttcgttatg
     5941 attttgccaa cagtaacctt tatgacggtt gatagtttgc gtgcagtacc tcgtcactac
     6001 cgcgaagcaa gtttggctat gggagctacc cgctggcaga cgatttggcg tgttattttg
     6061 aacgcggcta agccaggtat ttttacggcc gttatctttg gtatggcgcg tgcctttggt
     6121 gaagccttgg cgattcagat ggtagttggt aactcagcgg ttatcccgac ttccttgaca
     6181 acgccagcag cgactttgac ttctgttttg acaatgggta tcggtaatac ggttatggga
     6241 actgttcaga acaacgtcct atggtccttg gccctagtct tgctcttgat gagccttatc
     6301 tttaacatga ttatgaaatt tattacgaga gagggtaaga aaaactatgc acgctaaaaa
     6361 atttgataaa ttggcgactg gtattcttta ctcaatcgct ggtgtgattg tcgttatctt
     6421 aacgtcactg attctttaca tcttgctacg tggtctgcca catgtaagct gggatttttt
     6481 gacaagtaaa tcttcatctt acaaagctgg cggtggtatt ggaattcagc tctacaactc
     6541 attcttccta ttggttgtaa cgctcttaat ttctgttcct ttgtcaaccg gtgcaggtat
     6601 ttatttggct gaatatgcta aaaaaggtcc attgactaac tttatccgta cctgtattga
     6661 gattttgtca tccctaccgt ccgttgtcgt tggtttgttt ggttacttga ttttcgttgt
     6721 tcagtttgag tacggttttt ctatcttatc aggtgccttg gctcttacag tcttcaactt
     6781 accacagatg acgcgtaacg tagaggacag ccttcgtcac gttcaccata cacaacgtga
     6841 agcaggattg gctcttggtc tatcacgttg ggaaactgtt ctccatgttg ttattccaga
     6901 agcacttcca gggattgtaa caggtatcgt cttggcatct ggtcgtatct tcggtgaggc
     6961 agcagctctt atctacacgg ctggtcagtc agctccagct cttgactggt caaactggaa
     7021 tccattgagt gttactagtc cgatttctat cttccgtcaa tcagaaaccc ttgcggttca
     7081 tatctggaag gtaaacagcg aaggtacaat cccagatgca acccaagttt ctgctggttc
     7141 agcagctatt ctcttggtct tcatcttgat tttcaacctt tctgcacgtt atattggtaa
     7201 gaaacttcat gcgaaactga cttcagcagc ctagaagtct gacttataag gagaaaaaat
     7261 ggcagattac aactggaatg aacgccatat cattactttt tcagaggaga atatcgccct
     7321 ttcaacaaaa gatgttcatg tttactatgg taaaaatgaa gcgatcaagg gcattgatat
     7381 gcagttcgaa aaaaataaaa ttacagcctt gattggacca tcagggtgtg ggaaatcaac
     7441 ctatcttcgc agtcttaatc gtatgaatga tactattgat attgctaagg taaccggtca
     7501 aattctttat gaaggaatcg acatcaatag accagatatg aacgtttacg aaattcgtaa
     7561 gcatatcgga atggtattcc aacgtcccaa cccatttgca aaatctattt accgtaacat
     7621 cacattcgca catgaacgtg caggcgtgaa agataagcag gttttggatg agattgttga
     7681 aacttctctg aaacaagcag ccctttggga tcaggtaaaa gatgacttgc acaagtcagc
     7741 ctttacctta tcaggtggac agcaacagcg tttgtgtatt gcacgagcta tctctgtaaa
     7801 accacaaatc ttgcttatgg atgagcctgc atcagccttg gacccgattg cgactatgca
     7861 gttagaagaa actatgtttg aattgaagaa ggactacacc attatcattg ttacgcacaa
     7921 tatgcaacaa gcagctcgtg caagtgatta cacagctttc ttctatctgg gtgacttgat
     7981 tgaatatgat aagacagcaa acgtcttcca gaatgcaaaa ctcaagtcaa ctagtgacta
     8041 cgtttcagga cattttggat agaaagggaa caaatgacat cacctatttt acaagtaaaa
     8101 gatttgtctg tttactataa caagaaaaaa gccttgaata atgtgtcaat cgatttctat
     8161 ccaaatgaaa ttactgcttt gattggacca tctggttctg gtaagtcaac cctcttgcgt
     8221 tctattaacc gtatgggaga tttaaaccca gaggtaacat tgacaggtgc gattgattac
     8281 aacggaaaga acatatacag tcctcgtacg gatactgttg atcttcgtaa ggaaatcggt
     8341 atggtattcc aacaaccaaa cccattcccg atgtctatct acgagaatgt tgtttatggt
     8401 ttacgtatta acggtatcaa ggacaagcag gtcttggacg aagcagttga gcgttccttg
     8461 attggtgctt ctatttggaa tgaagtgaaa gatcgcttgc atgactcagc agttggactt
     8521 tcaggtggtc agcagcaacg tgtatgtgtg gcccgtgtat tggcaacaag cccaaaaatt
     8581 attttgttgg atgagccaac gtcagccctt gacccaatct cagctggtaa aattgaggac
     8641 acactttatg gtctgaaaga taagtataca atggtgctag taacacgttc tatgcaacag
     8701 gcatcacgta tttcagatcg aacaggtttc ttccttgatg gcgatttgat agaatacaat
     8761 ttgacaaaag aaatgttctt gaatcctgcc aataaagaaa cagaagacta cattacaggt
     8821 aagttcggct aaggcctata agaaaaagaa agaggttcac atgttaagag ctcaatttga
     8881 agaggaatta ggaaaacttc ataaccaatt ttacgccatg ggaaatgaag tattggctca
     8941 gattaataat gctgttcgtg cctttacaac tcatgatcgt gaattagcaa aagaagtcat
     9001 tgaagcagat aagaaaatta atgctttcga agtgaaatta gagaaaaaat cacttgaaat
     9061 cattgctctc caacaacctg tctcaacaga tcttcgtcgc gttatcacag tcttaaaagc
     9121 aactagtgat ttggagcgta tgggagacca tgcagtctca gttgctaaag cagctatccg
     9181 tatgaaggga gaagttcgca tcccagttgt tgaagaagaa atcaagaaaa tgggacgtga
     9241 agttcgtcaa ttagtagaag aaactttgga actctactta ggtgatgctg atatggagcg
     9301 cgcttatgaa gtagcagcac gtgatgaggt tgtcaatgaa ttctattcaa ctattcaaga
     9361 attgacaacg gaagaaatca agaagaatcc agaatccttg gttgctggtc gcgactatta
     9421 ccaagtttta acctacttgg aacgtgtcgg tgattatgct aaaaatattt gtgagtgggt
     9481 tgtttatcta cgcacaggtg atattacaga gttgtaattc agaagtccga gagggctt
//
LOCUS       DAZQCJ010000077         9537 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000077 DAZQCJ010000000
VERSION     DAZQCJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.078"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            13..780
                     /locus_tag="ACHU66_001615"
     CDS             13..780
                     /locus_tag="ACHU66_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR3389883.1"
                     /translation="MNKRERLEEITRLVNKKGTVRISEIVELLNVTDMTVRRDFIELE
                     EQGVLTKIHGGARSNKAFQYREYSHSEKHIQNKEAKQEIAERAAQLIEDGDTVFLGPG
                     TTVAFLAEAIKNSRLTVITNCMPVFTILSQKKTEEFQVFLLGGEYRQVTESFVGEITN
                     TSLEKMRFSKIFFSGNGLKGTDVMTSTLAEAYTQNIAIKHSLEKYLLLDASKIGKDDF
                     TSFCDLHDVTALVTDMNAKDENYQLLSKHIEIITTKL"
     gene            987..1409
                     /gene="lacA"
                     /locus_tag="ACHU66_001616"
     CDS             987..1409
                     /gene="lacA"
                     /locus_tag="ACHU66_001616"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFR3389884.1"
                     /translation="MTIIIGADPAGSKLKDVIKDYLLEKSYDFKDVSDGSDFVDTTLA
                     VVAEVNQQEGNLGIVIDAYGVGPFMTATKVKGMIAAEVSDERSAYMTRGHNNSRIITI
                     GSEIVGEGIAKNIVKGFVEGEYDGGRHQIRVDMLNKMC"
     gene            1436..1951
                     /gene="lacB"
                     /locus_tag="ACHU66_001617"
     CDS             1436..1951
                     /gene="lacB"
                     /locus_tag="ACHU66_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFR3389885.1"
                     /translation="MKIAIGCDHIVTYEKIAVVDYLKNQGYEVLDFGTYDNVRTHYPI
                     FGKKVGEAVASGQADLGICICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGKITGELLMCDIIEAFIKAEYKPTEANKRLIEKIAAVETLNQEQANPEFFTEF
                     LEKWDRGEYQD"
     gene            1961..2893
                     /locus_tag="ACHU66_001618"
     CDS             1961..2893
                     /locus_tag="ACHU66_001618"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFR3389886.1"
                     /translation="MILTITLNPSVDIAYQLDTFHLDTVNRVEKVQKTAGGKGLNVTR
                     VLKQIGEDVVATGFIGGEIGSYVKKQLTRNDIKNSFVEIGSETRNCIAVLHDGKQTEI
                     LEQGPTIQEHEALNFIEHLEIILNNVDVVVISGSLPKGLASNYYVEIVELCKKCGVAV
                     VLDCSGEALKNVLESQQKPTVIKPNTEELSQLIGKEVTDDIQELKSVLSGQLFQGIDW
                     IVVSLGAQGAFAKYKDKFYRVRIPKIKVVNPVGSGDSTVAGIAAGLVHALPEAELLKN
                     ANVLGMLNAQEEQTGYVNLVHSESLYSQIEVEEV"
     gene            2895..3872
                     /gene="lacD"
                     /locus_tag="ACHU66_001619"
     CDS             2895..3872
                     /gene="lacD"
                     /locus_tag="ACHU66_001619"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFR3389887.1"
                     /translation="MVVTADKRKYMEKVRNEAGIISALAFDQRGALKKMMAKHQTEEP
                     TVEQMEELKRLVSEELTQFSSSILLDPEFGLPASRARNEQCGLLLAYEKTGYDTSSTS
                     RLPDCLVEWSVKRLKEEGADAIKFLLYYDVDGDPYVNLQKQAYIERLGSECQAEGLPF
                     FLEILSYDETIEDNASVEFAKVKPRKVNEAMRVFSDERFGVDVLKVEVPVNMNFVEGF
                     GTGEVVYTKEEAAEYFRQQEASTNLPYIYLSAGVSAKLFQDTLVFAHEAGAKFNGVLC
                     GRATWAGVVPVYIEQGEEAAREWLRTVGRENIEGLDAVLSQTATSWLEK"
     gene            4061..4894
                     /locus_tag="ACHU66_001620"
     CDS             4061..4894
                     /locus_tag="ACHU66_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR3389888.1"
                     /translation="MFRIIQSLNNNAALVKNEHGEQAVVMGLGITFQKKKGDLIVKDK
                     IENIFSLKNDEAKENFLTLLKDIPLDFISATYTVINHLVETYHYPVQEYLYVTLTDHI
                     YCAYQAVLKNTYQESRLPNISAEYPLEYKMAREALAMFREKLLNDFPNDEIGRIALHL
                     INAKGETIHPTSEEHDISKKIIAEVEQILIENGIKRSKENSNFYDRFMIHLSYFLNYL
                     DRSHQSNESIASLEQYIKLQNPRAHQVGSQIFEMITSLVDEPVNDSERFYIVLYIQRL
                     L"
     gene            4926..5243
                     /locus_tag="ACHU66_001621"
     CDS             4926..5243
                     /locus_tag="ACHU66_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFR3389889.1"
                     /translation="MNREEITLLGFEIVAFAGDARSRLMEALAAAQKGDYAKAEELVE
                     AANACIVEAHHAQTSLLQKEAAGEDLAFSVTLMHGQDHLMTTLLLKDMMGHMIELYKR
                     GDK"
     gene            5243..6922
                     /locus_tag="ACHU66_001622"
     CDS             5243..6922
                     /locus_tag="ACHU66_001622"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFR3389890.1"
                     /translation="MNKLIEFIEKGKPFFEKISRNPYLRAIRDGFIAAMPVILFSTLF
                     LMVAFVPNIFGFTWSDEAVAAIMKPYGYTMGIVAVLVAGTTAKSLTDAFNRQLPKTNQ
                     INFISTMIASISGFLLLASDGIEGGFANGYMGTKGLLTAFLAAFITVNIYKVCVKNNV
                     TIRMPDEVPPNVSQAFKNVIPYALSIFVLYGIDLVTRQFLGTNVAEAILKLFEPLFTA
                     ADGYVGITIIFGAYALFWFVGIHGPSIVEPAIAAITYANIETNFQLLQAGQHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPLV
                     LNPVFFVPFILAPIANVWIFKFFVDTLKMNSFSVNLPWTTPGPLGIVMGTNFAPLAFA
                     LAILLVVVDVLIYYPFLKVYDEQILAEEQSGKVENSLKEKVAANFNTAKADAILEKAA
                     VETEISEQTNVLVLCAGGGTSGLLANALTKAAKEYGVPVTATAGSYGAHREILPEYQL
                     VILAPQVASNYDDMKQETDALGIKLAKTEGAQYIKLTRDGQGALDFVKQQF"
     gene            7157..8563
                     /gene="lacG"
                     /locus_tag="ACHU66_001623"
     CDS             7157..8563
                     /gene="lacG"
                     /locus_tag="ACHU66_001623"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFR3389891.1"
                     /translation="MVKTLPKDFIFGGATAAYQAEGATQTDGKGRVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLELSEQFGVNGIRISIAWSRIFPTGFGAVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPETLHSNGDFLNRENIEHFVNYAAFCFKEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHAKAVKLYKDAGYSGEIGVVHAL
                     PTKYPYDPSNPDDVRAAELEDIIHNKFILDATYLGYYSEKTLEGVRHILKVNGGELDL
                     RDEDFAALDAAKDLNDFLGINYYMSDWMRAHDGETEIIHNGKGEKGSSKYQIKGVGRR
                     ESPVDIPKTDWDWIIYPQGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDGTVYDD
                     GRIDYVKKHLEVISDAISDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAYWYKKVAETQVIE"
     gene            8628..9521
                     /locus_tag="ACHU66_001624"
     CDS             8628..9521
                     /locus_tag="ACHU66_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFR3389892.1"
                     /translation="MLELKNENLTVQFSELGGQIISIKDKDGIEYLWQGDPTYWSGQA
                     PVLFPICGSLRNDWAIYEPAERPTFTGTIPRHGLVRKMLFKNVKNTENSLEFSISSNE
                     ETVKNYPFEFELSINYSLFGKTIRTEYRMKNLEGHRKLPYFIGGHPGFNCPLLDGESY
                     EDYYLEFEKVESCTVPRSFPETGLLDLRDRRPFLENQKTLDLSYKLFEHDAITLDQLA
                     SRKVTLKSKNHQKKLSIAFDDFPYLVIWSTTNQSPFIALEPWSGLSTSLEESDIFNAK
                     RNISYVAPKEVDTKYFDIIIE"
BASE COUNT         3077 a         1585 c         2042 g         2833 t
ORIGIN      
        1 aaaggattaa acatgaataa acgagaacga ctagaagaaa ttacacgatt ggtaaacaaa
       61 aaaggcacag tccgaatttc tgagattgtt gaattgctaa atgttactga tatgactgtt
      121 cgacgtgatt ttattgaact ggaagagcaa ggtgtcttaa caaaaattca cggcggtgct
      181 agaagtaata aggcttttca atatcgggaa tactcccatt ctgagaaaca cattcagaat
      241 aaggaagcca agcaagagat agcggaaagg gctgcacaac tgatagaaga tggagacacg
      301 gtatttttag gtccaggaac aacagtggcc tttttggctg aggccattaa aaattctcgc
      361 ctaacagtca tcacaaactg tatgcctgtt tttaccatcc tatctcaaaa gaagacagag
      421 gagtttcaag tctttcttct ggggggagaa tatcgtcaag tgacagaatc ttttgtagga
      481 gaaattacca atacaagttt agaaaaaatg cgattcagca agattttctt ttcaggtaac
      541 ggacttaagg gaactgatgt catgacctct accctagcgg aagcctatac gcaaaatatt
      601 gccattaaac attctttaga aaaatatcta ctgctagatg cctcgaaaat tggaaaagat
      661 gattttactt ccttttgtga tttgcatgat gtaactgctc tagtgaccga tatgaatgca
      721 aaggatgaaa attaccaatt attaagtaag cacatcgaaa ttatcactac aaaattataa
      781 agttatcaaa accaacagaa aagtgttggt tttttgtttg tacacgaaaa aaaacaaaca
      841 aaaataaaac aaaatatgtt gactttgtgt ttgtttggtt gtataattta aacataaaaa
      901 gcaaaaacgc tttttctaag ttttgcaaaa caaagatttt ctgccaaata tggctacaac
      961 tttctatttt tgttaggaga taggttatga caatcattat cggtgctgat ccagcgggtt
     1021 caaaactaaa agacgttatt aaagattatt tgctagaaaa atcatatgac tttaaagatg
     1081 tcagtgatgg aagtgatttt gttgatacaa ctttggcagt agttgcagaa gttaatcaac
     1141 aggaaggaaa tcttggcatt gtgattgatg cttatggtgt tggtccattt atgaccgcaa
     1201 ctaaagtcaa aggcatgatt gctgcagaag tttcagacga acgttctgcc tatatgacaa
     1261 gaggacacaa caattcgcgt attattacaa tcggtagtga gattgtagga gaaggaattg
     1321 ctaaaaatat cgttaaagga tttgttgaag gtgagtatga tggtggccgt caccaaattc
     1381 gtgtcgatat gctaaataaa atgtgctaat tgttgtaaac aaaggagaat aagagatgaa
     1441 aattgcaatc ggttgtgacc acattgtaac ctatgaaaaa atcgctgtgg tagattattt
     1501 gaaaaatcaa ggatatgaag tgttggactt tgggacttat gacaatgttc gtactcatta
     1561 cccaatcttt ggtaaaaaag ttggcgaagc tgttgcaagc ggacaagcag atttgggtat
     1621 ttgtatctgt ggaacaggtg ttggaatcaa caatgctgta aacaaggttc ctggtgttcg
     1681 atctgccctg gttcgtgata tgacctccgc gctttacgcg aaggaagagt taaacgccaa
     1741 cgtcattggt tttggtggga aaattacagg tgagttgctc atgtgcgaca tcattgaagc
     1801 cttcattaaa gcagaataca aaccaactga agcaaacaaa cgtttgattg aaaagattgc
     1861 tgcagttgaa acgctcaatc aagagcaggc taatccagag ttctttactg aatttcttga
     1921 aaagtgggat cgtggagaat accaggacta ggaggctcac atgattttaa caatcacttt
     1981 aaatccgtca gttgatattg cctatcaatt ggataccttt catttggata cggtcaatcg
     2041 agtagaaaaa gttcaaaaaa cagctggtgg taaggggctc aatgttacac gagttttgaa
     2101 acaaatcggt gaagatgttg tagcaacagg ctttattggc ggtgagattg gaagttatgt
     2161 aaagaaacaa ctcactcgaa atgatattaa aaattccttt gtggaaattg ggagcgaaac
     2221 acgtaactgt attgcggttc ttcatgatgg caaacagacg gagattttag aacaaggacc
     2281 aactattcaa gaacatgaag ccttgaattt tatcgaacac cttgaaatta ttctgaacaa
     2341 tgtagatgtt gttgttattt caggaagttt acctaaggga cttgctagca attattatgt
     2401 tgagattgta gaactttgta aaaaatgcgg tgtagctgtt gttctggatt gttctggtga
     2461 ggcgctgaaa aacgttttag aaagccagca gaagccgact gtgattaagc cgaatacaga
     2521 ggagttatca caactgattg gtaaagaagt tactgatgat attcaagagt tgaaatcagt
     2581 tttgtctggt caacttttcc aaggaattga ttggatagtc gtctcattag gagcacaggg
     2641 ggcttttgcc aagtacaaag acaagttcta tcgtgtcaga attccaaaaa tcaaagtcgt
     2701 caatccagta ggatcaggag attcaactgt tgcaggtatt gcagcaggtt tggtacatgc
     2761 tttacctgaa gcagaattat taaaaaatgc taatgttttg ggcatgttaa atgcccagga
     2821 agaacagaca ggttatgtta atttagtaca ttcagaatca ttgtattctc aaatcgaagt
     2881 cgaggaggtt taagatggta gtaacagcag ataaaagaaa atatatggaa aaagtgagaa
     2941 atgaagcagg tattatttca gctttagctt ttgaccaacg tggagccctg aaaaaaatga
     3001 tggcaaaaca tcagacagaa gaacctactg tggaacagat ggaagaacta aagagactag
     3061 tttcggaaga attaacacag ttttcttcct caattctgtt agatcctgaa tttggtttgc
     3121 cagcatcacg cgctcgtaac gagcagtgcg gtttgctcct agcttatgaa aaaactggct
     3181 atgataccag ctctactagt cgtttaccag attgcctagt agagtggtct gtaaaacgac
     3241 taaaagaaga aggggcagat gcgattaagt ttctactcta ctatgacgtt gatggtgatc
     3301 catacgtcaa tcttcaaaaa caggcctata ttgaacgctt gggttcagaa tgtcaggctg
     3361 aaggtttacc attcttcttg gaaattttga gctatgatga aacgattgaa gacaatgcta
     3421 gtgtggaatt tgcaaaagtc aaacctcgaa aagtaaatga ggccatgaga gtattttcgg
     3481 atgaacgctt tggagtggat gttctcaagg tagaagtacc tgtgaacatg aattttgtag
     3541 aaggctttgg aacaggggaa gttgtctata caaaagaaga agcagccgaa tatttccgtc
     3601 agcaagaagc gtcaactaac ttaccatata tttatctaag tgcaggtgtt tccgctaaac
     3661 ttttccaaga tacactagtg tttgcacatg aagcaggtgc taaatttaac ggtgttctct
     3721 gcggtcgtgc gacgtgggcg ggtgttgtgc cagtctatat tgaacagggt gaagaagctg
     3781 cgcgtgaatg gttacgtacc gttggtcgtg aaaatatcga gggacttgat gcagtcttgt
     3841 ctcaaacagc aacttcttgg ctagaaaaat aattgaaaaa ataaccattt tctcaaaaga
     3901 aagggtttac attttctgag aatatgctat aataatctca tcagcaagaa tagagtggat
     3961 tgtaactaat tcgattaggc aagactacaa atggatttga gagtaggata ttctcggttt
     4021 gtttgtagtc tttttttgct taaaaataga ggagggatat atgtttagaa ttatacagtc
     4081 actaaataat aacgctgccc tggtgaaaaa tgaacatggg gaacaggcag tcgtgatggg
     4141 attaggtata acctttcaaa agaagaaagg ggatttaatt gttaaggaca agatagaaaa
     4201 tatcttttcg ctaaaaaatg atgaagccaa agaaaatttt ctaaccttat taaaagatat
     4261 tcccttagat tttatttcgg caacctacac ggttattaat catctggtag aaacttatca
     4321 ttaccctgta caagaatatc tatatgtgac actgacagat catatctatt gtgcatatca
     4381 ggcggtttta aagaatacct atcaagaaag taggttacct aatatttcag ccgaatatcc
     4441 attggaatat aaaatggcgc gtgaagcctt agctatgttt cgtgaaaagt tgctaaatga
     4501 ttttccaaat gatgagattg gtagaattgc acttcactta atcaatgcaa agggtgaaac
     4561 cattcatccc actagtgaag aacatgacat cagtaagaaa attattgctg aggttgaaca
     4621 aattttgata gagaatggga ttaaacgttc aaaagaaaat agcaattttt atgatcgctt
     4681 tatgattcat ttgtcctatt ttctgaatta cttagataga tcccatcagt caaatgaatc
     4741 gattgctagt ttggaacagt acatcaagct ccagaatcca agagcccatc aggttggaag
     4801 tcagattttt gagatgatta catcattggt tgatgagcca gtaaatgata gtgaaaggtt
     4861 ctatattgta ctatatattc aacggttatt gtaaatcaat tcaattttaa aaggaggttc
     4921 atattatgaa cagagaagaa attactttat taggttttga gattgttgca ttcgctggtg
     4981 atgctcgctc tcgtttgatg gaggcactgg ctgctgctca aaaaggtgat tacgcaaaag
     5041 cagaagaact agttgaagct gccaatgctt gtattgttga agcccaccat gctcagacaa
     5101 gtttgttgca gaaagaagcg gctggtgagg atttagcctt tagtgtgacg ctcatgcatg
     5161 gtcaagacca tctcatgacg acattattat tgaaagatat gatgggtcat atgattgaac
     5221 tttacaaacg aggtgataag taatgaataa attgattgaa ttcattgaga aagggaagcc
     5281 tttctttgaa aagatttcga gaaaccctta tttaagggct atccgtgatg gttttatcgc
     5341 tgccatgccg gttatcttgt tctcaactct ctttctaatg gtagcatttg tacctaatat
     5401 ctttggattt acttggtctg atgaagcagt tgcggctatc atgaaacctt acggttatac
     5461 aatgggtatt gtagctgtct tagttgcagg aacgaccgca aaatctttga cagatgcgtt
     5521 taaccgtcaa ttgccaaaaa ccaatcagat taacttcatt tcaaccatga ttgcctcaat
     5581 ttcaggtttc ttattactgg cttctgatgg tattgaaggt ggatttgcca atggttacat
     5641 gggaactaag ggacttttga cagcctttct ggcagccttc attacggtta atatctataa
     5701 ggtctgtgtg aaaaacaatg ttaccattcg tatgccagac gaagttccac cgaacgtatc
     5761 ccaggcattt aaaaatgtga ttccatatgc attgtctatc tttgtcttgt atggaattga
     5821 tcttgtgaca cgtcaattcc ttggaacaaa cgttgctgag gcgatcctga aattatttga
     5881 gcctctattt acagccgcag atggttatgt tggtattaca attatctttg gtgcctatgc
     5941 tttgttctgg tttgtaggga ttcatggtcc atctattgtg gaaccagcaa ttgcagccat
     6001 tacttatgcc aatattgaaa ccaacttcca gcttctacag gcgggtcaac atgcggacaa
     6061 aatcctgact tcaggtactc aaatgtttat cgtaacaatg ggtggtacag gtgctacctt
     6121 ggttgtgcca ttcatgttta tgtggttgac taagtctaaa cgaaataaag caattggacg
     6181 tgcttcagtt gttccaactt tctttggtgt aaatgaaccg atcttgttcg gtgcaccact
     6241 tgtactcaac ccagtattct ttgttccatt catcttagct ccaattgcta acgtatggat
     6301 tttcaaattc tttgttgata cgctaaaaat gaacagtttc agcgttaact tgccatggac
     6361 aactccagga ccattgggga ttgtaatggg gacaaacttt gctccacttg cctttgcact
     6421 agctatctta ttggtagttg tcgatgtact tatctattat ccattcttga aagtttacga
     6481 tgagcagatt ctcgctgaag aacaatcagg gaaagttgaa aatagtttga aagagaaagt
     6541 ggcggctaac tttaatactg ccaaagcgga tgctattctt gaaaaagctg cagtcgaaac
     6601 cgaaatttct gaacaaacaa atgttttggt actttgtgca ggaggtggga caagcggctt
     6661 gcttgccaat gctttaacaa aagctgcaaa agaatacggt gttccagtca cagcaacagc
     6721 aggaagctat ggggcgcatc gtgagatttt gccagagtat caattagtca ttctagcacc
     6781 acaagtggca tcaaactacg atgatatgaa acaagaaacc gatgcattgg gtattaaact
     6841 tgccaaaact gaaggggctc aatacattaa actcactcgt gatggtcaag gtgctcttga
     6901 ctttgtcaaa caacagtttt agaacctata ttcacagttc agcacttcca tctaaggcta
     6961 gtctttcagc tactcatcgt tagttttttt caaaatacag tcagtattcc ctcaaaaaac
     7021 tgccttgatt agcgaaaaac tatctcttat atacaagcac ttcacttgtg aatacaggtt
     7081 cttaatcaaa aggggaggga gttatcatct ccaactccta cccctttatt tatttttgat
     7141 agaagaaggt aggcatatgg ttaaaacatt accaaaagat tttatatttg gtggagcaac
     7201 tgctgcatat caagcagagg gtgctacaca gacggatgga aaaggtcgtg ttgcatggga
     7261 caagtatcta gaagataatt actggtacac tgcggaaccg gcttctgatt tttataatcg
     7321 ttatccagtt gatttagaac taagtgaaca atttggggta aacggcattc gtatttcaat
     7381 tgcgtggtct cgtattttcc caacaggctt tggagcagtg aatccaaagg gagtagagta
     7441 ttaccataaa ttatttgcgg aatgccataa acgtcatgta gaaccatttg taacgcttca
     7501 ccattttgat acaccagaaa cgctacattc taatggtgac tttctaaatc gagaaaatat
     7561 tgagcacttc gtcaattacg cagcgttttg cttcaaagaa ttccccgaag ttaattactg
     7621 gacaaccttt aatgaaatcg gtccaattgg tgatgggcaa tatctagttg ggaagttccc
     7681 accagggatt cagtatgact tagctaaggt attccaatct caccacaata tgatggtttc
     7741 acatgccaag gcagttaagc tctacaagga tgctggttat agtggagaaa ttggtgttgt
     7801 acacgcattg ccaacgaaat atccttatga tccaagcaac cctgacgatg tacgtgcagc
     7861 ggaactagaa gatattattc ataataaatt tatcctagac gcaacatact tgggttacta
     7921 ttctgaaaaa acgcttgaag gcgttcgaca tattctgaaa gtaaatggtg gggaattaga
     7981 ccttcgtgat gaagacttcg ctgccttaga tgccgcaaaa gatttgaacg acttcttggg
     8041 catcaattac tatatgagtg attggatgag agctcatgat ggagaaactg agattatcca
     8101 taatggtaaa ggggaaaagg gaagttctaa gtatcaaatc aaaggagtcg gtcgtagaga
     8161 gtctccagtt gatattccaa aaacggactg ggattggatt atctatccgc aaggccttta
     8221 tgatcaaatc atgcgtgtaa aaaatgacta tccaaactat aagaaaatat acatcactga
     8281 aaacggccta ggctataaag atgagtttgt agatggtaca gtctatgatg atggtcgaat
     8341 cgactacgtg aaaaagcatt tagaggtgat ttcagatgca atctcagatg gtgccaatgt
     8401 taaaggttat ttcatctggt ccttgatgga tgtattttct tggtcaaatg gatacgagaa
     8461 acgatatggt ttgttctatg ttgactttga gacccaagaa cgttatccaa agaaaagtgc
     8521 gtattggtat aaaaaagtag cggaaacaca ggttattgaa taaaactaat acaaggatgc
     8581 ctagctcaag ttagcatcct tgttttcata taagggaggc agtttttatg ctcgaattaa
     8641 aaaatgagaa cttaacagta caattttctg aattaggtgg gcaaattatt tctattaaag
     8701 acaaggatgg tatcgagtat ctttggcaag gagatccgac ctattggagc ggtcaagcac
     8761 cagttctatt tcctatttgt ggaagtttac gaaatgattg ggctatctat gaacctgcag
     8821 aaagaccgac atttacaggc acaattccaa ggcatggact tgtgagaaaa atgctgttca
     8881 aaaatgtaaa aaatactgaa aactctttag aattttctat ttcatcaaac gaagaaaccg
     8941 taaaaaatta cccttttgaa tttgaacttt ctattaacta ctcactattt ggcaaaacaa
     9001 ttcgaactga atatcggatg aaaaaccttg agggacatag aaagctacct tactttattg
     9061 ggggtcaccc aggttttaat tgtcccttgt tggatggtga gagttatgaa gactattatt
     9121 tagaatttga aaaagtagaa tcttgtacag tacctagaag ttttccagaa actggcttac
     9181 ttgatctgcg agatcgccgg ccattcttgg aaaatcagaa gaccttggac ttgtcctata
     9241 aactgtttga gcatgatgcc ataacattgg atcaattagc atcacgaaag gtaaccttga
     9301 aatcaaaaaa tcatcagaag aaactttcga ttgcatttga tgattttcca tatttagtga
     9361 tttggtcaac tacaaatcag agtcccttta tcgcattaga accatggagc ggactctcta
     9421 catcactgga agaatctgac atattcaatg ccaaacgaaa tattagttac gttgctccaa
     9481 aagaagttga tacaaaatat tttgatatta ttattgagta aataccatag ttaagta
//
LOCUS       DAZQCJ010000078         9396 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000078 DAZQCJ010000000
VERSION     DAZQCJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9396
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.079"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(89..715)
                     /locus_tag="ACHU66_001625"
     CDS             complement(89..715)
                     /locus_tag="ACHU66_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR3389893.1"
                     /translation="MELVYMDGRKEPYTLSSIVAECAEVQHHTVTRIIRKNLERFERF
                     GKVGFKIQAMESGQQSKDYILNEQQATLLITFLKNTEQVANFKENLVKAFFEMRDEVA
                     EFRYQRALEKPKRKALHEAIETWQEAPKHAHSTVTNLLLKGTTGMNKRQLVAHRGGHN
                     GIDSPTSQELIRYQALEDIAIAMINLGMTYQDIKNMVFRPLKNAPQGA"
     gene            complement(719..1462)
                     /locus_tag="ACHU66_001626"
     CDS             complement(719..1462)
                     /locus_tag="ACHU66_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HFR3389894.1"
                     /translation="MRRNYSKVIEEMRTTHGLNLVAIGQRIGTDPRTVGKWAKGKNKP
                     NQESRKRLNRLYREVKQDMTTQNTIEPFEDFYDWSKMKKKKPTTEVIATQQFGGKSFE
                     IYGNKDNPLFIAVEVAEMIEVQNTTDLLKRIDEDEKLTYVITRAGQKREVNMLTEFGL
                     YEVLFQSRKPKAKEFKKVVKNILKEIRVNGYYMQGELIQDQPPHPLPGISDLTYIKNK
                     LAEVQEMDNLADITAGLERVAKLVAVIGG"
     gene            complement(1481..1678)
                     /locus_tag="ACHU66_001627"
     CDS             complement(1481..1678)
                     /locus_tag="ACHU66_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR3389895.1"
                     /translation="MKNNMRVLLAKRREKVSHVAEATGVSKSTLTALYYERTKKPDIE
                     TLQKVANYLGVTIDELLTPEE"
     gene            1888..2415
                     /locus_tag="ACHU66_001628"
     CDS             1888..2415
                     /locus_tag="ACHU66_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR3389896.1"
                     /translation="MNRLKILRKEKGETQDQVAEVAGVSKRSYIYWENGERQIKPDKA
                     QALADHFGVPVGYLLGYSDTSELIKDMIPHAVVDDNGNPTSLEEIQNSVFTTSEDEAL
                     IRAGHLKALSILPGNLEKILNSDPESVYRMLAVAPADYQAIILLWAIMSSEQRKTTLA
                     MLQSFNFDDVTDQTT"
     gene            2951..4096
                     /locus_tag="ACHU66_001629"
     CDS             2951..4096
                     /locus_tag="ACHU66_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR3389897.1"
                     /translation="MKITEVTKKDGSIVYRASVYLGVDAITGKKAKTRVTGRTKTEVK
                     QKAKQAPLEFKKNGSTVKKVVEVKTYQELAELWLESYKLTVKPQTFVVTKRQLYNHLI
                     PIFGGMKLDKIVPATIQQFVNGLAPKLVNFKVIHSINNRILQHGVLLQVLATNPARDV
                     ILPKKQKKGREAVRFIEPEHLKQFLDHAEQLASTNFSKYYQYVIFKLLLATGCRIGEL
                     SALEWSDIDLEAGTISISKTYSMELHIIGTTKTKAGTRIISIDKKTALLLKQYRNRQR
                     LMFLEVGACAPSVVFATTVREYLPRISLQEIINRRCKALGIPRFTCHAFRHTHASLLL
                     NAGISYKELQYRLGHSNISMTLDTYGHLSKDKEKEAVSYYEKAMNSL"
     gene            4195..4638
                     /locus_tag="ACHU66_001630"
     CDS             4195..4638
                     /locus_tag="ACHU66_001630"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR3389898.1"
                     /translation="MKIRGFELVSQFTDENLLPKRETAHAAGYDLKAAERISLEPGEI
                     KLVPTGVKAYMQANEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPANEGHIFAQM
                     RNITEETVVVEAGERMVQAVFAPFLLADGDQADGVRTGGFGSTGK"
     gene            4645..5178
                     /locus_tag="ACHU66_001631"
     CDS             4645..5178
                     /locus_tag="ACHU66_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFR3389899.1"
                     /translation="MQILFVRHSEPDYSMFDQHDNPRLYAGFGRDLAPLTEKGRTLAQ
                     EIASNPIFSQAQVVVASSVTRALETATYIAHAQQLPLLVEPFFHEWRPDMTGQNASQD
                     EAVLAHRLFLENSGAVPESSPVRYETAAEMRERFLQALEKYKAYDRIVIVCHGMLIRQ
                     FVPKETIAYCEILEYTL"
     gene            5185..6555
                     /gene="radA"
                     /locus_tag="ACHU66_001632"
     CDS             5185..6555
                     /gene="radA"
                     /locus_tag="ACHU66_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR3389900.1"
                     /translation="MTIAKKKTTFVCQSCEYHSPKYLGRCPNCGSWSSFVEEVEVAEV
                     KNERVSLTGEKTRPMKLNEVSSIQVARTKTNMEEFNRVLGGGVVPGSLVLIGGDPGIG
                     KSTLLLQVSTQLSTIGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSIRTE
                     IEKIKPDFLIIDSIQTIMSPDISSVQGSVSQVREVTNELMQIAKTNNIATFIVGHMTK
                     EGTLAGPRTLEHMVDTVLYFEGERQHTFRILRAVKNRFGSTNEIGIFEMQSQGLVEVL
                     NPSEVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRAS
                     LIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVALASSYKDKPTNPQECFI
                     GEIGLTGEIRRVNRIEQRINEAAKLGFTKVYAPKNSLTGIKVPKEITVISVTTIGEVL
                     QKVFNL"
     gene            6676..7170
                     /locus_tag="ACHU66_001633"
     CDS             6676..7170
                     /locus_tag="ACHU66_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFR3389901.1"
                     /translation="MSYFQNFMKANKAYVDLHGDYHLPLRPKTKVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQLGTREIVVLHHTDCGAQTFTNEEF
                     AVQLKRDLGVDVAGQDFLPFTDIEESVREDIALLKQSPLIPDDVEISGAVYDVDTGRM
                     TEVI"
     gene            7378..8136
                     /locus_tag="ACHU66_001634"
     CDS             7378..8136
                     /locus_tag="ACHU66_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HFR3389902.1"
                     /translation="MSDNRMKYTIDSNMQFPLVEIALEAGESAYIQQGSMVYHTPSVT
                     LNTKLNARGGSGFGKLMGAIGRSMVSGESMFITQAISNSDDGKIALAPSTPGQVIALE
                     LGAEQYRLNDGAFLALDGSAQYKMERQSVGKAFFGGQGGLFVMTTEGRGTLLANAFGS
                     IKKLTLDGGSITIDNAHVVAWSRDLDYNIHMENGFLQSIGTGEGIVNTFTGYGEIYVQ
                     SLNIETFAQVIGNHIVGAGGDGGGGSTSLLDAIF"
     gene            8314..8760
                     /locus_tag="ACHU66_001635"
     CDS             8314..8760
                     /locus_tag="ACHU66_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HFR3389903.1"
                     /translation="MKVRIEFDDSLDHVEVVIRTSQLGPEIEQIQQALQQVSRPSLVF
                     YKGSSEYFLSLGDILFFETDGTKIFAHTGDDAYEVKMKLYELGEHLPIYFCRVSKSTI
                     ANARAVYSLDKSFSGTSRISFYQTHKEVHVSRHYYHLLKEKLREVR"
     gene            8765..>9396
                     /locus_tag="ACHU66_001636"
     CDS             8765..>9396
                     /locus_tag="ACHU66_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389904.1"
                     /translation="MKRLLIGAAVIGLAVLVLLRDSLGLHLAMPFWTTVWFVLTFVTG
                     FHRLVKLDWLASIFCFGLAFIAANSVYEWVEFGTWNMFLSLVLLCLGLKLIFKPRKRR
                     LAHGVVTGVIGDKGRDTAFGSSTRYIHEENFIHDSVDVAFGSSTVYFDQAVILGNKAD
                     FHIDAAFSNITLYLPADWQAVVELDSAMSTTNNYASEPKEKVLLVTGDMA"
BASE COUNT         2499 a         1971 c         2152 g         2774 t
ORIGIN      
        1 aataacagct tgcctgcttg ttaattttgt tttagttagt ggttaaaggc tttgctgatt
       61 ggtctcggta agcctttttt tgttgctctc acgcgccttg tggtgcgttt tttagtggtc
      121 tgaataccat gttttttata tcctggtacg tcatgcctaa gttgatcata gcaatagcta
      181 tgtcttctag cgcctggtat ctgataagtt cctgactggt tgggctgtca atgccattgt
      241 gtccgccacg gtgtgccact agctggcgtt tgttcattcc agtagttccc tttagcaaga
      301 ggtttgtaac agtgctgtgc gcgtgttttg gggcttcctg ccatgtttca atagcttcat
      361 gtaatgcctt gcgcttaggc ttttctagtg ccctctggta gcgaaactct gccacctcgt
      421 cacgcatttc aaagaatgct ttgactaggt tttccttaaa gtttgcgact tgctcggtat
      481 tctttagaaa tgtgatcagc aatgtcgctt gttgttcgtt taagatataa tcttttgact
      541 gttgcccact ctccatagct tggattttaa atccaacctt tccgaaacgt tcaaagcgtt
      601 caagattttt tctgattata cgtgtaacag tgtggtgttg aacctctgca cattctgcca
      661 caatgctact aagtgtatac ggctctttcc gtccgtccat gtaaaccagt tccattgact
      721 aacctccaat cactgccact agcttagcca ctcgctcaag acctgcggtg atgtctgcta
      781 gattgtccat ttcctgcacc tcagcaagtt tattcttgat ataagttagg tcactgatac
      841 ctggtaaggg gtggggcggt tggtcttgaa taagttcgcc ctgcatgtag tagccgttta
      901 ctcgaatttc tttcaagatg tttttgacta ctttcttgaa ctctttggct tttggcttac
      961 gtgattgaaa tagcacttca taaagtccaa actcagttaa catgttcact tctcgttttt
     1021 gacctgccct agttattaca taggtcagct tttcatcttc gtcaatcctt tttaataagt
     1081 cggttgtatt ttgtacttca atcatttcag ccacttcaac agcaataaaa agcgggttat
     1141 ctttgtttcc ataaatttca aaagactttc ccccaaattg ttgagtagcg ataacctcgg
     1201 tagtcggttt ctttttcttc atttttgacc agtcgtaaaa atcttcaaat ggttcgattg
     1261 tgttttgtgt tgtcatgtcc tgctttacct ctctataaag tctgtttaat cgttttcgac
     1321 tctcttggtt aggtttgttt tttccttttg cccacttgcc aactgttcgg gggtctgttc
     1381 cgatacgctg accaatagca accaagttca gcccgtgagt ggttcgcatt tcttcaatga
     1441 ctttactgta gttcctcctc attgcttgcc tgctcctttc ttattcttct ggcgttagta
     1501 gttcatcaat cgtcacgcct aaatagtttg cgactttttg aagcgtttca atatctggct
     1561 tcttggtacg ttcgtagtac aaagctgtca aagtactttt tgaaacgcct gtagcttctg
     1621 caacgtgtga aactttctca cggcgtttgg ctagcaatac ccgcatattg ttcttcatgg
     1681 tccttgctcc tttctctttg ataaccaacc tgaatagatt gaaaggtgtt gaggtcggtt
     1741 attgcacttt ttgttcaact tgtgttgacg attttgatta tattgcactt tttattcatt
     1801 gtcaatactt ttttttgaaa aagttgtatt ttttgttcac ttcttaaatt tttaatgata
     1861 taatcacaac tgaaaggtgt tgaaattatg aatagattga aaattttacg caaagaaaaa
     1921 ggcgaaactc aagaccaggt tgcagaagtt gccggagtga gtaaacgctc ttacatatat
     1981 tgggaaaatg gtgaaagaca aatcaaacca gacaaagccc aggcacttgc tgaccacttt
     2041 ggggtgcctg ttgggtattt gttggggtat agtgatactt cagaactcat taaggatatg
     2101 atcccgcatg cagtagtaga cgataacgga aaccctactt cactagagga aatacagaat
     2161 tctgtcttta ccacttcgga agatgaggca cttattcgtg ctggtcattt aaaagccttg
     2221 tctattcttc cagggaattt agaaaaaatc ctaaatagtg accctgaaag tgtttataga
     2281 atgcttgccg tcgctcctgc tgattatcaa gctatcattc tactttgggc aattatgtca
     2341 tcagaacaaa gaaaaacaac attggcaatg ttgcagtcat ttaacttcga tgatgtaact
     2401 gaccaaacta cttaaccact gcgctcccct acacccccct caccacgtcc aaacacttct
     2461 cgggtacatt taccctactg aactattcca cgcgctcagg gtcgttctag tggactgtgt
     2521 ggagtgaaaa aagtccgcat taaaaaaagt cagtttttat cagtagataa aaaatgtaag
     2581 gttttgttag gttgtatatt ttagcaacca tagcgaaact caacaaaact caatagctaa
     2641 aaaagtcaag ttttttcaag ttggatattt tagcaacctc aataaacctc aacttctata
     2701 tcggccgtat gttagcattt gttagcattc ggcgcgtgtg aactctaaaa tctcaccttt
     2761 tacaacccta tcaaacgctc caaaatcgtc tgtatcgtat tttctccttg gctggtataa
     2821 ttttacctgc ctacccaaaa caaacgaaaa taagggctat tctcataagc ctacgcatga
     2881 tataaaccta aaacattcta aaaccctttt aataacagct tgcctgctga tgatggaaag
     2941 gtttatgatc atgaagatta ctgaagtaac aaaaaaagac ggtagcattg tttaccgtgc
     3001 aagtgtctat cttggggtag atgccattac aggcaagaaa gcaaaaacca gagtaacagg
     3061 taggacaaaa acggaggtta aacagaaagc aaagcaagcg ccattggagt ttaaaaaaaa
     3121 cgggtcaacg gtcaagaaag tagtggaggt aaaaacctat caagaattgg ctgaactatg
     3181 gttagaaagt tacaagttaa cagtaaagcc tcagactttt gtagtaacca aaaggcaact
     3241 atataaccac cttattccaa tatttggggg aatgaaacta gataagatag taccagccac
     3301 cattcagcag tttgttaatg gtctggctcc taagttggta aattttaaag ttattcattc
     3361 aatcaataat agaattttac aacatggggt actgttgcaa gttctggcca caaatccagc
     3421 tcgtgatgtt atcttaccca aaaagcagaa aaagggacgt gaggcggtca ggttcataga
     3481 acccgaacac ttaaaacagt ttcttgatca tgctgaacaa ttagcaagta ccaactttag
     3541 caagtattac caatatgtta tttttaagtt attactggcc actggttgcc gtattggtga
     3601 attgtctgcc ctggagtggt ctgatattga ccttgaagcc ggcacaatat caatcagtaa
     3661 aacctatagc atggaactgc atataatcgg gacaacaaag acaaaggcgg gtactcgtat
     3721 catcagcata gacaaaaaaa cagctctctt actgaaacaa tatcgaaacc gccaacgctt
     3781 gatgtttctt gaggtcggcg cgtgcgctcc ctctgttgtc tttgctacaa cggttaggga
     3841 atacctccca cggatttcac tgcaagaaat tataaaccgt cggtgtaagg ccttgggtat
     3901 tcctcgcttt acctgtcacg ctttccgcca tactcacgct agtttactgt tgaacgctgg
     3961 tatcagttat aaggaactcc aatatcgtct tggacactca aacatttcaa tgaccttaga
     4021 cacttatggg cacctatcca aagataaaga aaaagaggca gtttcatatt atgaaaaagc
     4081 catgaatagc ctttagggtg aacaaaaagg tgaacaaatt gaaaaaacaa ctaattacaa
     4141 ggtgatagaa accttattaa atcaacgttt tgaaaggatg aaagaaaaaa tattatgaaa
     4201 atccgtggtt ttgaactggt cagccagttt actgatgaaa atttattgcc aaaacgtgag
     4261 accgctcatg cagcaggtta tgacctgaaa gctgcggaga ggatcagctt agagcctggt
     4321 gagattaagc tggttccaac gggtgtcaag gcctatatgc aggcaaacga ggtcctctat
     4381 ctctacgacc gttcgtccaa ccctcgcaag aaggggctgg ttttgattaa ctcagttgga
     4441 gttatcgacg gcgactacta tggcaatcca gccaatgaag gtcatatctt tgcccagatg
     4501 cgaaacatca cagaggaaac tgtcgtggtg gaagctggcg agcgaatggt gcaggctgtt
     4561 tttgcaccct tcctcttggc tgacggcgac caggccgacg gcgttcggac aggtggattt
     4621 ggctcgacgg ggaaatagac tactatgcag attctctttg tgcggcattc cgaacccgac
     4681 tacagtatgt ttgaccagca tgataatcct cggctgtatg caggttttgg acgggatttg
     4741 gctcctttaa ctgaaaaggg gcggacttta gctcaggaaa ttgctagtaa tcctatcttt
     4801 tcccaagctc aagtagtcgt cgcttcaagc gtcacacggg ccttggaaac tgcgacctac
     4861 attgctcatg cccagcaact acctcttctg gtcgagccct tttttcatga atggcgacca
     4921 gatatgacgg ggcaaaatgc cagtcaagac gaggctgtct tagctcatcg attatttttg
     4981 gaaaatagcg gagcggtgcc tgagtcttca cctgttcgct atgagactgc tgcggagatg
     5041 agagagcgat ttttacaggc ccttgaaaaa tacaaagcct atgaccgaat cgttatcgtc
     5101 tgtcacggta tgctgatacg ccagtttgtt cccaaagaaa ctatagccta ctgcgaaatc
     5161 ctagaataca ctctatagga ggaaatgacc atcgctaaga aaaaaacaac ctttgtctgt
     5221 caatcctgcg agtaccactc gcccaagtat ctgggccgtt gccccaactg tggctcctgg
     5281 tctagctttg tcgaggaagt ggaagtcgct gaagtcaaga acgagcgggt cagtctgaca
     5341 ggtgagaaga cccgtcctat gaagctcaat gaagtgtcgt ccattcaagt ggcccgcacc
     5401 aagaccaata tggaagagtt taaccgtgtc cttggtggcg gcgtggtgcc gggcagtctg
     5461 gtcctgattg gaggcgatcc agggattggc aaatcgacct tgctcctgca agtatcgacc
     5521 caactttcga ctattggcac cgtcctctat gtgtcggggg aagagtctgc ccagcagatt
     5581 aagttgcgag cggagcgttt gggcgacatt gacagcgagt tctatctcta tgcggagacc
     5641 aatatgcaga gcattcggac tgagattgag aaaatcaagc cagatttcct gattatcgac
     5701 tctatccaga ccattatgag ccctgacatt tctagcgtgc aaggttctgt cagtcaggtc
     5761 cgtgaagtga ccaatgagct tatgcagatt gccaagacca acaatatcgc aacctttatc
     5821 gtcggccaca tgaccaagga aggcaccctg gctggaccgc ggaccttgga gcacatggta
     5881 gacaccgttc tctattttga gggcgagcgt caacacacct tccgtatctt gagggcggtc
     5941 aaaaatcgct ttggctccac caacgaaatc ggtatttttg aaatgcagtc gcagggcttg
     6001 gtcgaagttc tcaatccaag tgaggtcttt ctggaagagc gtctggacgg agcgactggc
     6061 tcggctattg tcgtgaccat ggagggcacc cgccctatcc ttgccgaagt gcaggctctg
     6121 gtgaccccga ccatgtttgg caatgccaag cggaccacga caggcttgga tttcaaccgt
     6181 gccagcttga ttatggcggt tttggaaaaa cgggccggtc tgctcctcca aaaccaagat
     6241 gcctacctta agtcagcagg cggtgtcaaa ctggatgagc cagctattga cctagcggtc
     6301 gcagttgccc ttgcctccag ttacaaggac aagccaacca acccacaaga gtgctttata
     6361 ggcgagattg gcctgacagg ggaaatccgc cgcgtcaatc ggattgaaca acggattaac
     6421 gaagccgcca aattgggctt taccaaggtc tatgccccaa aaaattccct gacaggtatc
     6481 aaggtgccca aggaaatcac cgttatcagc gtgaccacca ttggcgaagt cttgcagaaa
     6541 gtattcaact tatgaaatca ttcgggagtg acagctagtc gctcctcttt ttgcgacaaa
     6601 tagtcgggaa tgtgataagc taggtctaac ctatttctga aaatttcaga aattctcatc
     6661 agacaaggag gtcctatgtc ttattttcaa aactttatga aggctaacaa ggcctatgtt
     6721 gacctgcatg gtgattacca tttgcctctt cgtccaaaga ccaaggtggc tatcgtgacc
     6781 tgtatggact cacggcttca cgtggctcag gccttgggct tggctttggg ggatgctcac
     6841 attctgcgga atgcaggtgg gcgcgtgacg gaggacatga ttcgctcgct ggttatttcc
     6901 cagcagcagc tcggcacacg ggaaatcgtg gtgctccacc atacagactg cggtgcccag
     6961 acctttacca atgaagaatt tgcggtccaa ctcaagcgag atttgggagt ggatgtggct
     7021 ggtcaggatt ttcttccttt tacagacatt gaagaaagcg tgcgggagga tattgctctc
     7081 ttgaaacaat ctccgctcat acctgacgac gttgaaattt ctggtgcggt ttatgatgtg
     7141 gatacaggtc gtatgacaga agttatatag gaatgctcag gaagttcggt ttttcctgta
     7201 aattttatct gaatgaccga gaaatccttg acaagttttt ttgaatgaga taaaattgta
     7261 ttatcacaac agaacaagcg gagagataaa tgatgcttgg tttattctag cttgttcttg
     7321 gtttattatg gctatcctgt ggttaacagg acctttcgta aaaaatagga gtcaactatg
     7381 tcagacaatc ggatgaaata tactatcgat agtaatatgc agtttccttt ggtggaaatc
     7441 gccttggaag ctggtgaatc agcttatatt caacagggga gcatggtcta ccatacgccg
     7501 agtgtgacct tgaataccaa gctcaatgcg cgtggtggtt caggttttgg taaattgatg
     7561 ggggccatcg gtcggtcaat ggtatcaggc gaatctatgt ttatcacgca agccatttcc
     7621 aatagtgacg atggaaaaat tgcccttgcg ccttcaacac caggtcaggt tattgccttg
     7681 gaacttgggg cagagcaata tcgactaaat gatggtgcct tcctggccct tgatggttct
     7741 gctcaatata aaatggaacg ccagagtgtt gggaaagctt tctttggtgg tcagggtgga
     7801 ctctttgtga tgacgacaga gggacgcgga acacttttgg cgaatgcgtt tggttctatt
     7861 aagaagctaa ctttggatgg tggaagtatc accattgaca atgctcatgt ggttgcttgg
     7921 agtcgtgatt tggactacaa tatccatatg gaaaatggtt tcctccaatc tatcggtaca
     7981 ggtgaaggaa tcgttaatac ctttactggt tatggagaaa tctatgtaca gagtttgaac
     8041 attgagacct ttgctcaggt cattggcaat catattgtcg gtgcaggtgg tgatggtggt
     8101 ggtggaagca catcgttgct ggatgcgatt ttttaagaag aaaaactctg caattttcaa
     8161 aatcatataa taaaaataaa gtccctcgcg taatgcaagg gacttttctt agtttatttc
     8221 taccacttac ctgtctaatt ttgccactta ctgaaatgcc cttgtaattg gatgtcgaac
     8281 tggtataata gagctatcaa aaaggaggga gctatgaaag ttaggattga atttgatgac
     8341 agcttagatc atgttgaagt tgttatccga accagccagc tgggaccaga gattgaacag
     8401 attcagcaag ctttgcaaca agttagtcgt ccctcgcttg ttttttacaa ggggagcagc
     8461 gagtattttc tttcattggg tgacatactt ttctttgaaa cggacggaac caagattttt
     8521 gctcatacag gcgatgatgc ctacgaggtt aaaatgaaac tctatgaact tggggagcat
     8581 ttgcccatct acttttgtcg ggtttctaaa tcaacgattg ccaatgctag agctgtgtat
     8641 tcattggata agtctttttc aggaactagt cggatttcct tctatcagac ccacaaggaa
     8701 gtccacgtgt cgcggcatta ttatcattta ttaaaagaaa agttacgaga agtgaggtag
     8761 ggagatgaaa cgtctattaa ttggagctgc agttatagga ctggcggttt tggtcttgtt
     8821 gagggattca ttgggtttgc atttggcaat gcccttttgg accactgtct ggtttgtatt
     8881 aacctttgtg acaggttttc accgcttggt aaagttggat tggcttgcca gtatcttttg
     8941 tttcggccta gcttttatag ctgccaactc tgtctatgaa tgggtggagt ttgggacttg
     9001 gaacatgttt ctgtcgcttg tcttactctg tcttggcttg aaactaattt ttaaaccaag
     9061 gaagcgcaga ctggctcatg gagttgtcac aggggtgatt ggcgacaagg gacgcgatac
     9121 agcttttggt tcctcgactc gttatatcca tgaggagaat tttattcatg atagtgtgga
     9181 tgtagccttt ggatctagta cagtctattt tgatcaggcg gtgattctgg gtaataaggc
     9241 agattttcat atcgatgcgg ccttctcaaa tatcacactc tatctgcctg ctgactggca
     9301 agcggttgta gagttggata gcgccatgtc aacaactaac aactacgcat ctgagccaaa
     9361 agaaaaggtt ctcttggtga cgggtgatat ggcatt
//
LOCUS       DAZQCJ010000079         9244 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000079 DAZQCJ010000000
VERSION     DAZQCJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.080"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(52..2841)
                     /gene="ileS"
                     /locus_tag="ACHU66_001637"
     CDS             complement(52..2841)
                     /gene="ileS"
                     /locus_tag="ACHU66_001637"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR3389905.1"
                     /translation="MKLKETLNLGKTAFPMRAGLPTREPEWQKAWDEANLYARRQELN
                     EGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFRAPFVPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLEMCRDYALSQVDKQRDDFKRLGVSADWENPYITLTKDYE
                     AAQIRVFGAMADKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GLLDTDSYIVVWTTTPFTVTASRGLTMGADIDYVLVQPAGSDRKYVLAEALVDSLAAK
                     FGWESFEVISKHKGAEFEYIVTEHPWDTEVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGVKYDLEVAVTVNERGLMNEAAGPDFEGQFYDKVVPTVKEKLGDLLLASEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDQIEATTFIPAWGKTRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVAALFEEHGSIIWWKSEAKDLLPAGF
                     THPGSPNGEFTKETDIMDVWFDSGSSWNGVMNARENLAYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAVLSQGFVLDGKGMKMSKSLGNTILPSDVEKQFGAEILRLWVTS
                     VDTSNDVRVSMDILGQVSETYRKIRNTLRFLIANTSDFNPASDAVAYEELRSVDQYLL
                     VKFNKLVAQIREAYDNYDFMGIYKSVVNFVTLDLSAFYLDFAKDVVYIDGAKSLSRRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEHEAEEFVQLAEMPEVETFANEAQLLA
                     DWENFMVFRTQAQKALEEARNAKVIGKSLEAHLTAYVSAETKSFLESLNADLAQLLIV
                     SNLTVTTEAAPESAVLVEEVAFTVERASGEVCDRCRRIDTSVAERSYGATICDHCASV
                     VEENFAEAVAQGFEA"
     gene            complement(2852..3295)
                     /locus_tag="ACHU66_001638"
     CDS             complement(2852..3295)
                     /locus_tag="ACHU66_001638"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR3389906.1"
                     /translation="MIRLELVNKDNFEAVLQVQLAPEDQRRVASVEYSLAQAWLYKDS
                     GMILPYAVVSGRKVVGFAMLSIETKDNSYYLWRLLIDKDFQNRGCGKEVVQQMIGKAK
                     ADPLCHKISINYVIGNHKMRYILEKFGFQPVGLDGQKMKMELIIK"
     gene            complement(3526..4215)
                     /locus_tag="ACHU66_001639"
     CDS             complement(3526..4215)
                     /locus_tag="ACHU66_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFR3389907.1"
                     /translation="MALTALELKDKTFATKFRGYDADEVDDFLDIVTRDYEDLIRKNH
                     DQELELKNLRERLAYFDEMKESLSKSVLLAQDTAEKVKVAAEDQAANIIKQADYDAAT
                     LLHEAKDKANEILRNATDNAKKVVIETEELKNQTRIFHQRLKSTVESQLSLVNSSEWE
                     EILRPTASYIQTSDEAFRDVLHKALDEELPVEEESLDYTRQLTPEEIAELTRHAAAFE
                     SGESVVLSIEE"
     gene            complement(4224..5015)
                     /locus_tag="ACHU66_001640"
     CDS             complement(4224..5015)
                     /locus_tag="ACHU66_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HFR3389908.1"
                     /translation="MKNDKNLLQHFSREEREFVEKIMDMCQQVEDTYSYRLTHFLHPR
                     QDEIVCKIANYYQLQTFSSRDLVSTEHSRVIIAPTYYELDIKHFELTALHLSYARKFH
                     TLSHSQVLGTFLNQLGIKREYLGDILIDDERLIVFIDQKFGQIALQSITKVARVPVKG
                     KEEEWTTVRLPIGQEWRSKDVLVSSMRLDKLISVAFNLSRATANKLIEAGHVKLDYVL
                     TEQTSKLVEIGQLISVRRYGRVRLNEFLGFSKQGKIKLKLDIVKT"
     gene            complement(5018..5281)
                     /locus_tag="ACHU66_001641"
     CDS             complement(5018..5281)
                     /locus_tag="ACHU66_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFR3389909.1"
                     /translation="MQILIFLLLKIVEIYSYLLLAYALLSWFPSLYTSSIGRLIQWLV
                     APILKPFKRFNLQFMGLDWTVMAAMIALNMGTRFLVQLLLLLA"
     gene            complement(5286..5855)
                     /locus_tag="ACHU66_001642"
     CDS             complement(5286..5855)
                     /locus_tag="ACHU66_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFR3389910.1"
                     /translation="MALKDTFKNLFNYFEVDEVNEVEEQADAYSMPNDRPKMRVANTT
                     VAPVREQQPKVETRREARSESQLQRLHERQQELMTNNNEKEIVKTTIDIKFPKRYEDA
                     PEMVNLLLDNASILIDFQYMSEQQARRCLDYLDGARSVLSGNLKKVSNTMWLLTPVNV
                     TVNIEELRNANAGTTTGAADSNFDFDIKR"
     gene            complement(5872..6543)
                     /locus_tag="ACHU66_001643"
     CDS             complement(5872..6543)
                     /locus_tag="ACHU66_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFR3389911.1"
                     /translation="MNFQKNKDAIFSAVAKAAEKAGRPSESVKVIAVTKYVDSSIANQ
                     LVNTGITHIGENRVDQFLEKYNALADKQLTWHLIGTLQRRKVKDIIPFVDYFHALDSV
                     KLAQEIDKRATKIVKCFLQVNVSGEESKHGFAVSEIDAALAEISLLENIELVGLMTMA
                     PIDATDDELDEIFSKIQEIQEELATRNLPRMPFTELSMGMSGDFERAIAHGATYVRIG
                     SAFFE"
     gene            complement(6550..7779)
                     /gene="ftsZ"
                     /locus_tag="ACHU66_001644"
     CDS             complement(6550..7779)
                     /gene="ftsZ"
                     /locus_tag="ACHU66_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR3389912.1"
                     /translation="MAFSFEAAASHGAVIKVIGVGGGGGNAINRMIEEGVAGVEFIAA
                     NTDVQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEALTNVLTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKNLGALTVAVVTRPFGFEGNKRGNFAIEGIEGLREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MENKGNALMGIGIGTGEDRVIEAARKAIYSPLLETTIDGAEDVIVNVTGGYDMTLTEA
                     EDASEIVNQAAGQGVNIWLGTSIDETMKDEIRVTVVATGVRQDTADKPARHRMEAVSP
                     RPSQRFDHSVASAPTRGAAQQTEAPKASAFGEWDLRRENLIRPTDTEPTSTVSVEKFT
                     MDHDEDELDTPPFFRNR"
     gene            complement(7805..9184)
                     /gene="ftsA"
                     /locus_tag="ACHU66_001645"
     CDS             complement(7805..9184)
                     /gene="ftsA"
                     /locus_tag="ACHU66_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR3389913.1"
                     /translation="MARNGFFTGLDIGTSSVKVLVAEYIDNEMNVIGVSNVKSAGVKD
                     GIIVNIEVAAGAIKKAIEQAEEKSGIRIEKVNVGLPANLLQIEPTQGMIPVTTDSQEI
                     TDLDVENVVKSALTKSMTPEREVISFIPEEFTVDGFQGIKDPRGMMGIRLEMRGMLYT
                     GPRTILHNLRKTVERAGVQVENIVISPLALTRSVLNEGEREFGATIIDLGGGQTTVAV
                     MRGQELQYTNIYQEGGDYITNDISKVLTTSKSIAENLKYNYGIAYPQDASDKEKFTVE
                     VIGENAPVEVTERYLSEVIAARLRQIFDRVKQDLERTRALDLPGGIVIVGGGAILPGI
                     TELAQEVFGVNTKLFVPNQIGIRNPAFASVISFVEYVGELEDVENIAQHAVNGETTLR
                     HKPVEIPVARPRISQPIQREIVNELNTVDQVEEQVAPIYHDEEEEVRPAAKGNITDRI
                     RGLFGSMFE"
BASE COUNT         2636 a         2143 c         1660 g         2805 t
ORIGIN      
        1 gtagaaaaaa gcctatcgct agatagactt taacaaatgt attgtgattg cttaggcttc
       61 aaaaccttga gctactgctt cagcaaagtt ttcttccact acgcttgcac agtggtcaca
      121 gattgttgcg ccatagctgc gctctgcaac actagtatca atacggcggc aacggtcaca
      181 tacttcaccg cttgcacgct ctacagtaaa ggcaacttct tctaccaaaa cagcactttc
      241 aggagctgct tccgtggtta ctgtcaagtt tgaaacgatg agcaactgag caaggtctgc
      301 attcaagctc tccaagaagg acttggtttc agctgagaca taggcagtca aatgagcttc
      361 aagtgactta ccaatcactt tggcattgcg tgcttcttcc aaggcttttt gtgcctgagt
      421 acggaagacc ataaagtttt cccaatcagc taaaagctgc gcttcattag caaaggtttc
      481 cacctctggc atttccgcca actgaacaaa ttcttctgct tcgtgttcca agtaagacca
      541 gatttcttct gccgtatgtg gtaagattgg cgtcaagagc ttggtaattt tcaccaagat
      601 gtcatagaag acagtttgca tctgacgacg agaaagtgat ttagcaccat caatgtagac
      661 gacatctttg gcaaaatcga ggtagaaggc tgacaaatca agggttacaa agtttactac
      721 agatttatag atgcccatga aatcataatt gtcgtaggct tcacggattt gagctaccaa
      781 tttattgaat ttcaccaaga gatactggtc aacagaacga agctcttcgt atgccaccgc
      841 atcacttgca ggattgaaat cagaagtgtt ggcaattaag aagcgaaggg tattacggat
      901 tttacggtag gtttcagaaa cctgtccaag aatatccata gacacacgga catcgtttga
      961 agtatcaacg gatgtcaccc acagacgcaa gatttctgca ccaaactgct tctcaacatc
     1021 acttggaagg atagtatttc ctagggattt agacatcttc atacctttac cgtccaatac
     1081 aaagccctgt gacaagacag ccttgtatgg cgcatgacca tttacagcaa cagaggtaat
     1141 caaggatgag ttaaaccaac cacggtattg gtcagaacct tctaggtaga ggtctgctgg
     1201 gtaggcaagg ttttcacggg cgttcatgac accattccaa gatgaaccag agtcaaacca
     1261 tacgtccata atgtctgttt ctttggtaaa ttcgccattt ggtgaacctg gatgtgtgaa
     1321 accagctggc aagagatctt ttgcttctga tttccaccag atgatagaac catgctcttc
     1381 aaaaagggca gcaacgtggt ctgtcacttc cttggtcata atcgctgttc catcttctgc
     1441 atagaagatt ggaagtggca caccccaagc acgctgacga gagatgaccc agtcaccacg
     1501 gtcacggatc atgttgtaaa gacgtgtttt accccaagca ggaataaagg tcgttgcttc
     1561 gatttggtcc aagatttcct gacggaattt agaaacagag gcaaaccact gtggcactgc
     1621 acgccagatg attggcttct tggttctcca gtcaaatggg taggagtgat tgatcacttc
     1681 actagcaagg agtaagtcgc ctaatttttc cttaaccgtt ggtacaacct tgtcatagaa
     1741 ttgcccttca aaatcaggac ccgcagcttc attcatcaag ccacgttcgt tgacagttac
     1801 cgcaacctcc aagtcatatt tgacaccgac attgtagtca tcctcaccaa aacctggagc
     1861 cgtatggacg ataccagtac ccgagtccgt tgtgacatgg tcacccaaga taaccaattc
     1921 atcaacttca gtatcccatg ggtgttcggt cacgatatat tcaaattcag cacccttgtg
     1981 ttttgaaatc acttcgaagg attcccaacc gaatttggct gccaagctat caaccaaggc
     2041 ttctgccaag acatacttac ggtcagagcc agcaggttga accaagacat agtcaatgtc
     2101 tgctcccatt gtcaaaccgc gagaagccgt tactgtaaat ggagtggttg tccagacaac
     2161 gatgtagctg tctgtatcca agagcccctt gccatctttg accttgttgg cataatacag
     2221 agatgttgag tcaatgtcat ggtattcgat ttcagcttct gcaagggcag attcagatga
     2281 ccatgaccag tagacaggct tagcaccgcg gtagatatag cccttgtctg ccatagcacc
     2341 gaagacacgg atctgagctg cttcgtagtc ttttgtcaaa gtgatatatg gattttccca
     2401 gtctgcagag acacccaagc gtttaaaatc atcacgttgc ttgtcaactt ggctaagagc
     2461 ataatcacga cacatttcaa gatattcaac taggtccatt tccttgcgtt taacaccttg
     2521 ttttgccaag acttgctcaa taggaaggcc gtgcgtatcc caacctggta caaatggagc
     2581 acggaaacca gacattgact tagagcgaac gataatatct ttagaaatct tgtttaaagc
     2641 atgtccaacg tggatatttc cgttagcgta tggagggcca tcgtggaggt ggaaggcagg
     2701 tttgccttca ttgagttctt ggcgacgagc atacaagttt gcttcatccc aggccttttg
     2761 ccattctggc tcacgtgttg gtaagcctgc acgcatagga aatgctgtct tacctaaatt
     2821 gagtgtttct tttagtttca tcataactcc tttattttat aattaattcc attttcattt
     2881 tttgaccatc taaaccgaca ggctgaaagc caaatttttc taagatgtac cgcattttat
     2941 gattaccaat gacataattt atagaaattt tatgacaaag cggatccgcc ttcgcctttc
     3001 caatcatttg ctggacgact tcctttccgc atccacgatt ttgaaaatcc ttatcaatca
     3061 acaagcgcca taaataataa ctattatctt tggtttcgat agataacatt gcaaaaccaa
     3121 caacttttct gccagatacc acagcatatg gtagaatcat ccccgaatct ttatataacc
     3181 aagcttgggc taatgaatat tcaactgatg ctactcggcg ttggtcctca ggtgcaagtt
     3241 gaacttgtaa cactgcttca aagttatcct tgtttactaa ttctaatcgt atcataaaaa
     3301 taaaaaaact cccgccaaat aaggacgaaa gttcgtggta ccaccttagt ttagatagag
     3361 tttcccctat ccctctatgt tcgtaacgtg aacagacgtc tctccttact attttcagga
     3421 aaaatcaaac aaagtgatga tccatagaaa gagattgtag ggcttccacc atctcctact
     3481 cgcttaaaat atttctaacg accttgtctt tgcttatgat ttctattatt cttctattga
     3541 aagtactaca gattctccac tctcaaaagc tgctgcatga cgagttaatt ccgcaatctc
     3601 ttctggtgtc aattgacgtg tataatccaa actttcttct tcaacaggta attcttcatc
     3661 caaagcctta tgaagaacat cacgaaaggc ttcgtcactt gtttgaatgt aacttgctgt
     3721 tgggcggagg atttcctccc attcagatga attaaccagt gataattggc tttctactgt
     3781 tgatttcaga cgctgatgga aaatacgtgt ctggtttttt aattcttcag tttcaatgac
     3841 aacttttttc gcgttgtcag tcgcattacg aagaatttca tttgccttat ctttagcttc
     3901 atgtaacaat gttgccgcat catagtcagc ttgtttaata atatttgccg cttgatcttc
     3961 ggccgcaacc tttactttct cagctgtatc ttgagctaaa agaactgatt tactcaatga
     4021 ttctttcatc tcatcaaaat aagccaaacg ttcccgcaaa tttttcaatt ctaactcttg
     4081 atcatgattt ttacgaatta aatcctcata atcacgtgtt acaatgtcca aaaagtcatc
     4141 aacttcatct gcgtcgtatc ctctaaattt tgtagcaaag gttttatctt ttaattctaa
     4201 tgctgtaagt gccatctttc ctcctaagtt ttaacaatgt ctaactttaa ttttatcttc
     4261 ccttgcttag aaaaacctaa aaattcattt aggcgaactc taccatatct cctaacacta
     4321 attaattgcc ctatttcaac taatttactg gtttgttctg tcagaacata atccaacttc
     4381 acatgtccag cctcaatcaa cttgtttgca gttgcccttg aaagattaaa tgcgaccgaa
     4441 atcaatttat ccaatctcat actagaaacc aacacatcct ttgaacgcca ttcctgtcca
     4501 atcggtagtc gaacagtcgt ccattcttct tcctttccct taacagggac acgagcaact
     4561 ttagtgatag attgtaaggc aatctgccca aatttctgat cgataaagac aatcaatcgt
     4621 tcatcatcaa tcaaaatatc acccaaatac tcccttttaa tccctagttg attgagaaaa
     4681 gttccaagaa cttgtgaatg ggataaggtg tgaaattttc tagcatagga caaatgaaga
     4741 gcggttaact caaagtgctt gatgtccaac tcataataag tcggggcaat aataacccgc
     4801 gaatgttctg tcgaaacaag gtcacgacta gaaaacgttt gtaactggta gtagttagca
     4861 attttgcata caatttcatc ttgtctagga tgtaaaaagt gagttaatct gtatgaatac
     4921 gtatcttcaa cttgctgaca catatccatt attttctcta caaactctct ttcttcccta
     4981 gaaaaatgtt gcaataagtt tttgtcattt ttcataacta tgctaacagt agcaataatt
     5041 gaaccaagaa gcgtgttccc atattaagcg caatcatggc tgccattacg gtccaatcca
     5101 accccataaa ttgtagatta aatcgcttaa agggttttaa aatcggtgct accagccatt
     5161 gaatcaaacg accaatcgag cttgtgtata ggctaggaaa ccaacttagc aaagcgtatg
     5221 ccaataagag ataagaataa atctctacaa tttttaatag taaaaaaatc aaaatttgca
     5281 tagcgttacc gttttatatc aaaatcaaaa tttgaatcag ccgcaccagt agttgtacca
     5341 gcattagcat tgcgcaattc ttcaatattg actgtgacat ttactggagt taatagccac
     5401 atggtatttg agaccttttt taaatttcca gataatactg aacgagcacc atccaagtag
     5461 tctaggcaac gtcttgcttg ttgctccgac atatattgaa aatcaatgag aatactcgca
     5521 ttgtctaata aaagatttac catctctggt gcatcttcat aacgttttgg aaacttaata
     5581 tcaattgtag ttttgacaat ctctttttcg ttattattag tcatcaattc ttgctgcctt
     5641 tcatgtaggc gttgaagttg actttctgaa cgtgcttccc gacgtgtctc tacttttggc
     5701 tgctgttcgc gcacaggtgc aactgtagta ttggcaacac gcattttagg acgatcatta
     5761 ggcattgaat aggcatctgc ctgttcttca acttcattga cttcgtcaac ttcaaagtaa
     5821 ttaaataagt tcttaaatgt atcttttaat gccatatttt cctcctctcc attattcaaa
     5881 gaatgctgag ccaattctca catatgtagc tccgtgagca atggcgcgtt caaagtcccc
     5941 actcattccc atacttaact ctgtaaaggg catacgtggt aagttccttg tagctagttc
     6001 ttcttggatt tcctgtattt tagaaaaaat ctcgtctaat tcatcatctg tagcatcaat
     6061 cggtgccatc gtcatcaatc caacaagttc aatgttctcc aataaactaa tttcagcaag
     6121 tgccgcatca atctcagata ctgcaaagcc atgcttactc tcctcaccgg aaacgttgac
     6181 ttggaggaaa cacttgacta ttttagtcgc acgtttatct atttcctgag ctagtttgac
     6241 agagtctagg gcgtggaaat aatcaacaaa tggaataatg tccttgacct ttcttctctg
     6301 taaagtccct atcaaatgcc atgtcaattg tttgtctgct agggcattat acttttctaa
     6361 gaactgatcg actcgatttt ctccaatatg agtaattcca gtattcacta gctgattagc
     6421 aatagagctg tcaacatatt tggtgacagc tattaccttt actgattcac ttggacgacc
     6481 tgccttctca gcagcttttg caactgctga aaagatagca tccttattct tttgaaaatt
     6541 cattttagtt tagcgattac ggaagaatgg tggtgtatcc aattcatcct catcatgatc
     6601 cattgtaaat ttttcgactg aaacagtgga tgttggttct gtgtcagttg gacgaatcaa
     6661 gttttcacga cgcaaatccc actctccgaa ggcagaagct ttcggcgctt cagtttgttg
     6721 tgcagctcca cgagttggtg ctgatgctac tgaatgatca aagcgttgag aagggcgtgg
     6781 tgaaacagct tccatgcgat ggcgggctgg tttatcagca gtatcttgac gaacacccgt
     6841 agccacaacg gtcacacgaa tctcatcttt cattgtttca tcaatagatg tacccaacca
     6901 aatgttaaca ccttgacccg cagcctgatt aacgatttct gaagcatctt ctgcttctgt
     6961 cagagtcatg tcatagccac ctgtaacatt gacaatgaca tcctctgcac catcaattgt
     7021 tgtttcaaga agaggagaat aaatcgcttt acgagccgcc tcaatcactc ggtcctcacc
     7081 agtaccgata ccgataccca tcaaggcgtt tcctttgttt tccataactg tcttcacatc
     7141 tgcaaagtcc aagttaatca aaccaggatt tgtaatcaag tctgtaattc cttgaacacc
     7201 ttgacgaaga acattatcag cttcgcttag tgcttctaaa agcggtgttt tcttatcaac
     7261 aatttcaaga aggttgttgt ttgaaataat taaaagtgta tctacttgtt cacgaagacc
     7321 ttcaattccc tcaatcgcaa agtttccacg tttatttcct tcaaaaccaa acggacgagt
     7381 cacaactgcg acagtcaacg cacccaagtt tttagcaata cgtgcaataa caggagccgc
     7441 accagtccct gagccaccgc ccataccagc agtgatgaag accatatctg caccagttaa
     7501 tacattagtc aatgcttctt cgctttcctc agcagctttc cgaccaacct caggttgacc
     7561 tccagcaccc aaaccacgag tcaatttagg acctagttgg ataactgttt cagctttaga
     7621 gctacttagc gcttgtacat ctgtgtttgc tgcaataaac tcaaccccag caacaccttc
     7681 ttcaatcata cggttaatcg cgttcccgcc accaccgccg acaccgatta ctttaatgac
     7741 agcaccatga ctagctgctg cttcaaatga aaatgccata gtttaaatca ccttttcttt
     7801 tctcttattc aaacatgctg ccaaataaac cacgaatgcg atctgtgatg tttcccttag
     7861 cagctggacg aacttcttct tcctcatcat ggtaaatagg agcaacttgt tcctctacct
     7921 gatcaactgt gttcaattcg ttcacgattt ctctttgaat tggctgacta atccgaggac
     7981 gagcaacagg aatttcaact ggcttatgtc tcaaggtagt ttcaccattt actgcatgtt
     8041 gggcaatatt ttctacatct tctaactcac caacatactc aacaaagcta atcacacttg
     8101 caaatgctgg attgcggata ccaatctgat ttggaacaaa aagcttagtg ttaacaccaa
     8161 acacttcttg ggccaattct gtaattcctg gtaagatagc accaccacct acaataacaa
     8221 tgcctcctgg taaatctaga gcacgtgtac gctccaaatc ttgtttcaca cggtcaaaaa
     8281 tctgacgcag acgtgccgca atcacttctg aaaggtagcg ctcggtcact tcaactggag
     8341 cgttttctcc aatcacttcg acagtgaatt tttccttatc acttgcatct tgcggatagg
     8401 caatgccgta gttgtatttc aagttttcag caatactctt tgaagttgtc aatacttttg
     8461 aaatatcgtt tgtgatataa tctccacctt cttggtagat gtttgtgtat tgcaactctt
     8521 ggccacgcat aacagccaca gtcgtttgac caccaccaag atcaatgata gtcgcaccaa
     8581 actcacgttc accttcattc aatacagagc gagttagtgc caacggagaa attacaatat
     8641 tttcaacttg gacacctgca cgctccaccg ttttacgaag attgtgaaga atggtacgtg
     8701 gacctgtata cagcatacca cgcatttcca agcgaattcc catcattcca cgagggtcct
     8761 taatgccttg gaatccatcg acagtgaact cttctggaat aaatgaaatc acttcgcgtt
     8821 ccggagtcat actctttgtc aatgctgatt ttacaacatt ctcaacatcc aagtctgtaa
     8881 tttcttgtga atctgttgta acaggaatca tcccttgagt cggttcaatc tgaagaaggt
     8941 tggcaggtaa tccaacattt accttttcaa tgcggatacc tgacttttct tctgcctgct
     9001 cgatcgcttt tttaatcgct ccagcagcca cctcaatatt tacaataatt ccatctttga
     9061 cgccagcact ttttacattg ctgaccccaa tcacattcat ttcgttatca atatattctg
     9121 caaccaagac tttaacggag cttgtaccaa tatctaatcc cgtaaaaaag ccgtttctag
     9181 ccattctatc agtcctctct tacttccaac tttaacaaat catcttccat tttgaaaaat
     9241 agta
//
LOCUS       DAZQCJ010000080         9059 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000080 DAZQCJ010000000
VERSION     DAZQCJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9059
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.081"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            23..1054
                     /locus_tag="ACHU66_001646"
     CDS             23..1054
                     /locus_tag="ACHU66_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR3389914.1"
                     /translation="MATITKRGNSYRATVSLYKKGEYKRETKTFSNRKDAELWTLEME
                     LEKGRGKNIAERSTLFPDFYRNWVHTVKKTDVREATFINYKRTLVIVDDLFDGIQLKH
                     LDDLVMQKKIDQYAETHSKKTVKELVLKIRGSLKYAYARGLISNDFGHLLKSKGQEQP
                     KSITEFKKLRQYCLSHTENEFNVLVALALETVARRGELLGIKKEDIFEYGIKILRSIS
                     PTNDDTRLKTKYSKRDISINEDVYQAVTKLAQTKEGYIFDWNGFKQASQLQKLLKQLG
                     LTKTTFHGLRDTHASFLFSKDISLDYISRHLGHNSILTTQQYYLELMPEKKHQQDADA
                     LSLLNDLSL"
     gene            complement(1280..1822)
                     /locus_tag="ACHU66_001647"
     CDS             complement(1280..1822)
                     /locus_tag="ACHU66_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389915.1"
                     /translation="MFNLLNKKAEVSKVAEYWNDTLIERGILSADELLEGKCWRCKSS
                     HGVAVCQIVSSKWSKDASLANQMVLCLSCQHEKPNVADTEIVWQWLEVENDERYWTLQ
                     GMAEYEKMYKKSVLQELWDMGIRDGEEVETLVNKVTSLSRKNDIVLNRATLAGLFRCE
                     IEQMRRKAFLNWTGIFKLVS"
     gene            complement(1842..2312)
                     /locus_tag="ACHU66_001648"
     CDS             complement(1842..2312)
                     /locus_tag="ACHU66_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389916.1"
                     /translation="MKRMTEISWNDIYKEWETYANHFGLTTPINTENLRDQKSKDFGK
                     GSLITLDLLADYDTDSEKTAAIWVASFCRDLIQDYAYLLNGRAYLTVNQIYFQALKQF
                     QSGAVIWSKPLTRLQPKLFVSYRLLENLDLSHYSCVVELAMLQASMVRTQILEK"
     gene            2444..3244
                     /locus_tag="ACHU66_001649"
     CDS             2444..3244
                     /locus_tag="ACHU66_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR3389917.1"
                     /translation="MILGDILKEYRSKNKLSMDKFAELSGLTKGYISMLEKNQHPKTK
                     KALLPTMDTLEKVAKGMAISVGELIEQLDSNQDIALTITPSQFKLFQQPIHPEVQTLD
                     NELKGDFHAQWLTFGQEQLAAMQTTSITPSASILHLDDYRERIDLPVPGKVSAGTGYW
                     QDTDMNNLVSFYNDEIPDNQSYDTIAQVVGDSMAPHIEDGDYLFIRLTPHIPLNTIGI
                     FSVNGENFVKKFKGDYLQSLNSDYDDIYFSPEDDIRPIGQVVDIYSPD"
     gene            complement(3907..5067)
                     /gene="mutY"
                     /locus_tag="ACHU66_001650"
     CDS             complement(3907..5067)
                     /gene="mutY"
                     /locus_tag="ACHU66_001650"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR3389918.1"
                     /translation="MLDLKEYGIEMWPEEKIQAFRKALLDWYDANKRDLPWRRTKDPY
                     AIWVSEIMLQQTRVDTVIPYYERFLHYLPTISDLAQAPEEVILKLWEGLGYYSRVRNM
                     QKAAQQMVEDFDGQFPTTHAAISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLSR
                     LFEVDYDIGLPANRKIFQAMMELLIDPERPGDFNQALMDLGSDIESPVNPRPQDSPVK
                     AFSAAYLNGTMDKYPIKAPKKKPIPVAYQGFLIRNKDNQFLLEKNNEAGLLSGFWSFP
                     LLEKGAIVEKQVSLFEVAEEVVQPDIRQSFTELYGLTVDWQEQEFGIVQHIFSHRKWQ
                     IEMVEGVAETLNLPESKELKWVSVEDFPTYPFAKPQQKMWENFIKEKEKQKS"
     gene            complement(5302..5694)
                     /locus_tag="ACHU66_001651"
     CDS             complement(5302..5694)
                     /locus_tag="ACHU66_001651"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HFR3389919.1"
                     /translation="MKVLDKLNLQPITVLDDYQATMLNEKEVLLTTKVTEKSLNPYGM
                     AHGGFLFTLADSVAGLTTVAGGSYSVTLQSNIHYMKAAKLGDTLSVTGSCTHDGSRTK
                     VVEVKIKNQDKQLLASASFTMFVTGKVE"
     gene            complement(5678..7420)
                     /locus_tag="ACHU66_001652"
     CDS             complement(5678..7420)
                     /locus_tag="ACHU66_001652"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3389920.1"
                     /translation="MKQSSFKELVRLVLGQPFQMYLMLVGTLVQVLLTVYLPILIGQA
                     VDAVLIADGQVLLGIIGKMVMVILLNTLVQWYLPLVTNRLVYGMVADLREQVYVKLHQ
                     MPLSYLDRHSVGDLVARFSSDSEQLTNGLLMVFNQFFIGILTIFLTIFTMARLDLTMM
                     LLVVALTPVSLLVARYIAKKSYGYYRKQTQARGKQSQLLEESISQLTLVQSFNAQEQF
                     TQGFQVVNDQYATYSQQAIFASSTVNPSTRFINAIIYALLAGLGALRIMAGQFTVGSL
                     TTFLNYASQYSKPFNDISSVLSELQSALACADRLFAILALDSIDDQAERKIDSEELKG
                     AISFDNVSFSYSPERSLIEHLDIDVEAGQKVAIVGPTGAGKSTMINLLMRFYDVTAGQ
                     IVLDGVPIRQYSREDLRRQIGMVLQETWIKSGTIHDNIAYGYPNATRELVIEAAKAAN
                     ADFFIRQLPQGYDTVLADGGEGLSQGQRQLLSIARVFVKIPKILILDEATSSIDTRTE
                     ILVQEAFAKLMEGRTSFIIAHRLSTIQSADLILVMVDGKIVEQGNHEALMAEQGVYYQ
                     MQTSQQTEEDESVR"
     gene            complement(7407..>9059)
                     /locus_tag="ACHU66_001653"
     CDS             complement(7407..>9059)
                     /locus_tag="ACHU66_001653"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3389921.1"
                     /translation="ELLVPLIIAYIVDTIIPNGSQGNLVAMLFLLVGLACIGIIVSLI
                     AQYYSAKAAVGVTKELTNDLYQKVLSLPKSSRDILSSSSLLTRLTSDTLQIQTGINTF
                     LRLFLRAPIVVFGSLIMAFYISPSLSAYFLEMIILLIFIVTVISVMTSRIYQSMRKEL
                     DGLVGQVRETVTGWRVIRAFGQREREIKAFQGINQIYKKQQLQAGFWSSLLSPLTFLV
                     VNGTLLILIWQGNIAISHNLLEQGMLVALINYLLQILVELVKMIMVVSTLNQTYISAQ
                     RIQEVFDQTSEDVESSLPKVVSEDKEIIFSVRHISFSYPKSAEESLSDIAFDLRKGQF
                     MGIIGGTGSGKSTLVDLLQALYSVPTNQLSLFIDGKSPKNLKEWRQQIAIVPQQAQLF
                     AGTVRSNLSLGLESVLDSELWAALEIAQAKSFIEDKGGLDSPVEAFGKNFSGGQRQRL
                     TIARAILQKAPILVLDDATSALDYLTESRLLAAIRRAFPAQTLIMVSQRTNSLRTADQ
                     ILVFDQGRQVGLGRHEDLLRSSAIYQEIDQSQHREEDSHETI"
BASE COUNT         2811 a         1946 c         1615 g         2687 t
ORIGIN      
        1 tttttataga aaggctgata aaatggcaac aataacaaaa cgtggtaact cttaccgtgc
       61 aactgtatca ctttataaga aaggagaata taaacgagaa actaaaacct ttagcaacag
      121 aaaagatgct gagctttgga cattagaaat ggaacttgaa aaaggtcgtg gcaagaacat
      181 tgccgaacga tctaccttat ttcctgattt ttatagaaat tgggtccata cggtcaagaa
      241 aactgacgtt cgcgaagcca cctttattaa ctataaacga actcttgtaa tagttgatga
      301 cctttttgat ggtattcaac ttaaacacct tgacgatctc gtcatgcaaa agaaaatcga
      361 ccaatacgct gaaacccact ctaaaaaaac agtaaaagaa cttgttctta aaatccgtgg
      421 ttcattaaaa tatgcgtatg ctcgtggttt gattagtaat gactttggtc atcttttgaa
      481 atcaaaagga caagaacaac ctaaatctat cacagaattt aaaaaactca gacaatactg
      541 cctaagtcat actgagaatg aatttaatgt tcttgttgct ctcgcccttg agacagtagc
      601 aaggcgcgga gaacttttag gaattaaaaa agaagatatt tttgaatatg gtatcaaaat
      661 tcttcgatca attagtccaa ctaatgacga tacccggctt aaaaccaaat attctaagcg
      721 tgatatttcc atcaacgaag atgtttatca agctgtgaca aaacttgcac aaacaaaaga
      781 aggctatatc tttgattgga atggattcaa gcaagctagt caacttcaaa agctgttaaa
      841 acagttggga ttaacaaaaa cgacatttca tggacttcgt gacacgcatg cctcatttct
      901 cttttcaaaa gatattagcc tagactatat ttctcgtcat ctaggtcata actctatctt
      961 aacaacacaa cagtattatc tagaattgat gccagaaaaa aagcaccagc aagatgccga
     1021 tgctttaagt ctcttaaacg acttatcatt ataattaaca ccaactggca ccaaaaaagc
     1081 gccataatcg ttggtataat aaggttttga ctaacgtttt tgagctataa ttcaaaaaca
     1141 ttcactccaa tttttattag ctgaaatata atcctataaa cgcatcatta gacttctttc
     1201 tttaagattc taagctgatg cgctttttat ttatctgtta ctccaatcag catgagatgt
     1261 tttcctacaa ctgcatcagt taactgacta gcttaaaaat tcctgtccaa tttagaaatg
     1321 cctttcgacg catttgttca atttcacagc gaaataatcc tgctaaggtt gctctattta
     1381 aaacaatatc attcttacga gaaaggctag ttactttatt tacaagcgtc tcgacttctt
     1441 caccatctct aattcccata tcccataact cttgaagtac tgatttttta tacattttct
     1501 catattctgc cataccttgt agagtccagt aacgttcatc attctcaact tctaaccatt
     1561 gccacacaat ttctgtatca gctacattag gtttttcatg ctggcaggat agacagagga
     1621 ccatctgatt agccaggctc gcatccttgc tccatttgct tgatacaatc tgacacacag
     1681 ctacaccatg agaactttta catctccagc attttccttc aagcaattca tcagcgctca
     1741 aaattcccct ttctatcaaa gtatcattcc aatactctgc aactttcgat acttcagctt
     1801 tcttattcaa caaattaaac attttcattc tcctactata attacttttc taatatctgt
     1861 gtccgaacca tactagcctg caacattgct aactcaacta cacaagaata atgactcaaa
     1921 tcaagatttt ccaacaaacg ataagataca aataacttcg gttgcaatcg tgtgagaggt
     1981 tttgaccata ttaccgctcc tgactgaaat tgtttgagtg cctgaaaata aatctgattg
     2041 actgtaagat aggctctacc atttagtaaa tatgcgtaat cttgtattaa atctctacaa
     2101 aatgaagcta cccaaatagc tgcggtcttc tcagaatcag tatcgtaatc ggctaataag
     2161 tccagcgtaa tcagacttcc ttttccaaaa tccttcgatt tctgatctcg caaattttca
     2221 gtatttatcg gagtcgtcaa accaaaatga ttagcatagg tttcccactc tttataaata
     2281 tcattccaag aaatctctgt catccttttc atttgcgact cctatttttc ttttttgtga
     2341 gattatcata acaccgtttt gattttttgt caaacgtgtt aaacaaaatt tgcaaaaaat
     2401 attttttaat ggtataattg gttaaacaga taaggagaca accatgattt taggtgatat
     2461 tttaaaagaa taccgaagca agaataaatt atcaatggat aaattcgctg aattatctgg
     2521 tttaacaaaa ggttatattt ctatgttgga aaagaaccag catcccaaaa ccaaaaaagc
     2581 tctcctgcca acgatggata cgctagaaaa agtcgctaag ggcatggcta tatcggttgg
     2641 tgagctaatc gagcaactag attctaatca ggatattgct ttaacgatta ctccttccca
     2701 gtttaaactc tttcaacagc ctattcatcc agaggttcaa accttggaca atgaattaaa
     2761 aggtgatttc catgcacaat ggttaacttt tggtcaagaa cagttagcag ctatgcaaac
     2821 tacctcaatt actccttctg cttctattct tcacttggat gactatcgag agcgaatcga
     2881 tttacctgtt cctggtaagg tttctgccgg tacaggttac tggcaggata cagatatgaa
     2941 caatcttgta tcattctata acgatgagat tccagataac caatcttatg atacaatcgc
     3001 tcaggttgtg ggagattcta tggctccgca tatcgaggat ggagactatc tcttcattcg
     3061 cctaactcct catattcctc taaatacgat tggaattttt tcagtcaatg gggaaaattt
     3121 tgttaaaaag ttcaaaggtg actatttgca atcactcaat tctgactatg atgatatcta
     3181 ttttagccca gaagacgata ttcgacctat tggtcaagtt gtagatattt atagtccaga
     3241 ttgatttcta agcctagtta atcactagtt atattggcta cattcatcca tctagttttg
     3301 gaaagtctgt tcgtatagct ggcgtttttt cctactgaaa acaaactgaa aagcacagta
     3361 aaaggaggct atgacagcct cccttttata tgtcaaattg gaaaagtcca ttaccagata
     3421 gttatttgac aacaagttaa tgattaaata attattctgc tacccttatg tctttgattg
     3481 tcctcttact atttgtctaa agtgtggaga ttgcttatta tgatgaaaat cgctagaggt
     3541 ttttgtgatt ttctaaagtt atgttcaaaa gttacaacat cttgaacaca tagtagttac
     3601 gacacgaata atcttagcct acttctgatt gaaccgggta gcaaggctaa ataatgagtt
     3661 aatgctatgc atttgacaat ggatatcaca aatagatata aaacagggat aacggacttt
     3721 tcccctacta gcatccctct ctttttatta gagaaagatg gctaatagta tatacacttc
     3781 tacgaacgct aaagaactcc tttcagcaat tcagatggcg ttcaacatct taactaacta
     3841 tttccttttt tcaattagat tataccacta ttgtaaaacg ctttcaaata attatgtgct
     3901 ccaaagtcaa cttttttgct tttctttttc cttgataaag ttttcccaca ttttttgttg
     3961 cggcttagca aatgggtagg taggaaaatc ttctacagaa acccatttca attcttttga
     4021 ttctggaagg ttaagtgttt ctgccacacc ttctaccatt tctatctgcc atttgcggtg
     4081 actaaaaatg tgctggacga tgccaaattc ttgctcttgc caatcaacag tcagaccata
     4141 aagttccgta aagctctgcc gaatatctgg ctgaacaact tcttctgcaa cttcaaagag
     4201 agagacttgt ttttcaacga ttgctccttt ttccaaaagc ggaaaagacc agaaacctga
     4261 tagaagacct gcttcattat ttttttccaa taaaaattga ttgtctttat tcctgatgag
     4321 aaatccctgg taggccacgg gaatcggctt tttctttggt gctttgattg gatacttatc
     4381 cattgtaccg tttaagtagg ctgcgctaaa ggctttgact gggctgtcct gtggacgtgg
     4441 attaactggc gattcaatat ccgaccctag atccattagg gcttggttga aatcgcccgg
     4501 tcgctctgga tcaatcaata gctccatcat cgcttggaaa atcttacgat tggcaggaag
     4561 accaatgtca taatcaacct caaacaagcg gctgagaacc cgcatcacat tgccatctac
     4621 cgctggttct ggcaaattaa aagcaatact ggaaattgct ccagcagtat agggaccaat
     4681 gccctttagg cttgaaatgg ctgcatgcgt agtcggaaat tgcccatcaa aatcttctac
     4741 catttgctga gctgcctttt gcatattccg cacgcgcgaa taatagccca atccttccca
     4801 gagtttgaga atgacctcct ctggtgcttg tgctaaatca ctaatcgtag gcaagtaatg
     4861 gagaaaacgt tcataataag gaatgaccgt gtctactctt gtctgttgaa gcatgatttc
     4921 agaaacccat atagcatacg gatccttggt ccgtctccaa ggcaagtcac gtttattggc
     4981 atcataccaa tctaagagtg ccttgcgaaa agcttgaatc ttttcctccg gccacatttc
     5041 gattccatat tctttcaaat ctaacataca tctagtataa cacaaagaaa gggagccagc
     5101 ttttgccaac tcccttatat cgtttataat caaaatcaaa ctagctccaa aggtttgggg
     5161 aacctttgga ggttggaaat tgagcgaacg aagttcattt ctacaaaggt ttggggaaac
     5221 ttgggaggtt ggaaatagag cgaacgaagt ttttttctct caatctagcc aagactgtct
     5281 gcttttgatg ttcgaagagt tttattctac ttttcctgtt acaaacatgg taaagcttgc
     5341 tgaagcaagt agctgtttat cttgattttt tatcttcact tcaacgacct ttgttctact
     5401 tccatcatgt gtgcaagaac cagtgacgga taaggtatca ccaagttttg ccgccttcat
     5461 ataatggata ttggactgca gggtgacaga ataagaaccc ccagccactg ttgtcaatcc
     5521 tgctactgaa tctgctaaag taaagaggaa gcctccatga gccataccat atggattgag
     5581 agatttttcc gtcacctttg tggtcagtag cacctctttc tcattgagca ttgtagcttg
     5641 atagtcatct aaaaccgtaa tcggttgtaa gtttaattta tctaacactt tcatcctcct
     5701 ctgtctgctg acttgtttgc atttgataat aaacgccttg ttcagccatc aacgcttcat
     5761 gattcccttg ttcaacaatt tttccatcca ccatgaccaa aatcaagtca gctgattgaa
     5821 tggttgatag gcgatgagca ataataaagc tggtccgtcc ttccatcaat ttggcaaatg
     5881 cttcttgtac taaaatttct gtccgtgtat caatagagga agtcgcctca tctaaaatca
     5941 ggattttagg tatcttgaca aagacacgcg caatagacaa gagctgacgt tgcccttggg
     6001 ataagccttc tcctccatct gccaaaactg tatcataccc ttgcggaagc tgacgaataa
     6061 agaagtctgc attagctgcc ttagccgcct caatgaccaa ttcccgcgtg gcatttggat
     6121 agccataggc aatattatca tgaatggtac cagatttgat ccatgtctcc tgtaaaacca
     6181 tcccaatctg tctgcgaaga tcctcacgac tgtactgtct aatcggaacc ccatctaaaa
     6241 caatctgacc agccgtcaca tcgtagaagc gcataagcag gttaatcatg gttgatttac
     6301 cggcaccagt aggtccaaca atggctacct tttgtccagc ttcgacatca atgtccaaat
     6361 gttctatcag agaacgttca gggctatatg agaaagacac attgtcaaag gaaatagctc
     6421 ccttcaactc ctctgaatca attttccgct cagcctgatc gtcaatgcta tctaaagcca
     6481 agatagcaaa cagcctgtct gcacaagcta aggcgctctg caattccgat aggaccgagg
     6541 aaatatcatt aaagggcttg ctgtactgac tagcataatt gagaaaagtt gtcagtgaac
     6601 ccactgtaaa ctgccctgcc ataattctca gcgctccaag tcctgcaagt aaggcataaa
     6661 taatagcatt gataaaccgc gtgcttggat tgaccgttga agaggcaaag atagcctgtt
     6721 gggaataggt cgcatactgg tcattcacaa cttgaaaacc ttgcgtaaat tgctcttggg
     6781 cattgaaaga ttgaaccaag gtcaattgac tgatcgattc ctcaagtagc tgagattgct
     6841 tcccacgtgc ctgtgtttgt tttctataat aaccatagga tttcttagcg atatagcgtg
     6901 caacaagcag agaaactgga gtcaatgcca caaccagcaa catcatcgtc aagtcaagcc
     6961 tagccatagt gaaaatggtt aggaaaatgg tcaagatacc tatgaaaaat tgattgaaaa
     7021 ccattaagag tccgttggtt aattgctcac tatcacttga aaaacgagct actaaatcac
     7081 caactgaatg acgatcaaga taagataggg gcatctggtg cagcttaaca taaacctgtt
     7141 cacgcaagtc agccaccatt ccataaacca agcgattggt tactagcggg agataccatt
     7201 gcaccagcgt attaagcagg ataaccatca ccattttgcc aattatccct agcaagacct
     7261 gaccatccgc tatcaatact gcatcaactg cctgaccaat caagataggc agataaactg
     7321 tcagaagcac ctgcactaaa gttcccacca acatcaagta catttgaaag ggttggccta
     7381 aaaccaatct gaccagttcc ttaaaactag attgtttcat ggctgtcctc ctccctatgt
     7441 tgtgattgat cgatttcttg gtaaatggca gagcttctca gcaagtcttc atgacggcca
     7501 agtcccactt gacggccttg atcaaagacc aaaatctgat cagctgttcg taaactattg
     7561 gttctctgag ataccattat taaggtttga gcaggaaatg cacgacgaat cgcggccaat
     7621 aaccgactct ctgtcagata atccaaagca gaggtcgcgt catctaaaac caatatcggt
     7681 gctttctgta aaatagcgcg tgcaatggtc aaacgttgac gctgtccacc agaaaagttc
     7741 ttcccaaagg cttcgacagg actatccaag cctcctttgt cctcaatgaa ggacttagcc
     7801 tgtgcgattt ctagcgctgc ccataactca ctgtccaata cactttctaa gcctaatgac
     7861 aggttagaac gcacagtccc agcaaaaagc tgagcctgtt gcggaacaat ggcaatttgc
     7921 tgccgccatt cttttaaatt cttaggactt tttccatcaa tgaagagaga gagctgattt
     7981 gttggcactg aatagagtgc ctgcaataaa tctactaggg tggacttacc tgacccagtt
     8041 cccccgataa ttcccataaa ctgtccctta cgcaagtcaa aagcaatgtc agacagggac
     8101 tcctcagcag attttggata agaaaaggaa atatgacgca cagaaaagat gatttcctta
     8161 tcctcactaa ccacttttgg caaactagac tctacatcct cagatgtttg gtcaaaaacc
     8221 tcttgaatac gttgcgctga aatataggtc tggttgagag tagacacgac cataatcatc
     8281 ttgactaatt ctactaaaat ttgtaaaagg tagttgatga gagcgaccaa catcccttgt
     8341 tccaacaaat tgtgtgaaat ggctatattt ccctgccaga tgagaatgag caaggtgccg
     8401 ttgaccacta aaaatgtcaa tggcgataaa agactagacc aaaaaccagc ctgcaactgt
     8461 tgcttcttat aaatctgatt tatgccttga aaagccttaa tttcccgctc tctttgtcca
     8521 aatgcacgaa tcactcgcca accagtcact gtctcacgta cctgccccac caaaccatct
     8581 aattcttttc gcatcgattg ataaatacgg ctggtcataa cagaaatgac tgtcactata
     8641 aaaatcaata ggataatcat ttctagaaaa taggcagata gacttgggct aatatagaat
     8701 gccatgatga gcgaaccaaa tacgacaatt ggagcacgta aaaacaaacg aagaaaagta
     8761 ttaatacctg tttgaatttg taaagtgtcg ctggtcaatc gtgtcagcaa gctggatgag
     8821 gaaagaatat ctcggctaga cttgggaagg gaaagaacct tctgatacaa gtcatttgtc
     8881 agctctttag tcactccaac cgctgcttta gctgaataat attgcgctat tagtgaaaca
     8941 ataattccaa tacatgctag accaaccagt aaaaaaagca tagcaaccag gtttccctgg
     9001 ctgccatttg gaataattgt atcaacgata taggcgataa ttaatggtac caaaagctc
//
LOCUS       DAZQCJ010000081         9015 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000081 DAZQCJ010000000
VERSION     DAZQCJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9015
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.082"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            139..1941
                     /gene="pepF"
                     /locus_tag="ACHU66_001654"
     CDS             139..1941
                     /gene="pepF"
                     /locus_tag="ACHU66_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFR3389922.1"
                     /translation="MSKQRYEIEEKYQWDLTTIFPTDDAWEAELADLQAETEKTKEFA
                     GHLLDSAKSLLEISETQLSLMRRIEKLYVYASMKNDQDTREGKYQEFQAKAMGLYAAF
                     SQAFAFYEPEFMAITEEQLEAFKAEEPTLVQYSHQFEKLLAAKDHVLSQEVEEVLAAT
                     SEIFESPAETFSVLDNASLRFPEIADEDGHLVPLSHGNYIHFMESKNRDVRQEAYEAM
                     YGTYEQFQHTYAKTLQSNVKVNNLKARLRKYDSARHASLAANFIPESVYDTLVAAVNK
                     HLPLLHRYINLRKKLLGIDDLKMYDMYTPLSDFSTKISYEDALKKCQETLEIFGEDYS
                     AIVKEAFENRWIDVHVNEGKRSGAYSGGAYDTNAFMLLNWQDNLDNMYTLIHETGHSL
                     HSMLTRKTQPYVYGHYSLFLAEIASTTNENLLTEKLLAEVEDDKERFAILNHYLDGFR
                     GTVFRQTQFAEFEQAIYKADQEGQVLTADFLNELYGQTNEKYYGLSAEENPEIQFEWA
                     RIPHFYYNFYVYQYATGFAAASALANKIVNGSPEDKENYLNYLKAGSSDYSLNVIAKA
                     GVDMTKEDYLNDAFKVFEARLEELEALVEKGVHL"
     gene            1943..2383
                     /locus_tag="ACHU66_001655"
     CDS             1943..2383
                     /locus_tag="ACHU66_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389923.1"
                     /translation="MISYKRSIVIPSVISLVFILLGLGGLTRPEPSMGSVVYLIFLQA
                     LLVYLYILRKQKAVFILTKQYLEFQEHFWSERQRHSLEEIAEIRYIVSPVYRGYQSKR
                     LRIVGNKGGLLAVVSLNRLDGADFNDIYHFVEQVAPHIRWDFSN"
     gene            2399..2947
                     /locus_tag="ACHU66_001656"
     CDS             2399..2947
                     /locus_tag="ACHU66_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6630 family protein"
                     /protein_id="HFR3389924.1"
                     /translation="MLTEEQLQDIFELADLMSNGDRELFLQLREVVFASDPHEILNFM
                     ERILDSESFDDFLDRVGESEKENLWLILIKLLEHLNYICVRDYKDNLEDFIYFFDCLQ
                     QVRNAGISLKLDSGGLSPIASISEWARVIDNKYLDENFCLGAVDMDTDSYYLFFSKQA
                     TFIRLQELAGNLGYRIDYAKNM"
     gene            3022..3741
                     /locus_tag="ACHU66_001657"
     CDS             3022..3741
                     /locus_tag="ACHU66_001657"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFR3389925.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDFMRTRQAQNTGFLKELEEFA
                     HQENIPVIPHETVAYFRLLMQTLQPKRILEVGTAIGFSALLMADNSPHSKITTIDRNE
                     EMIGFAKENFAKYDYRQQIELLEGEAMDILPTLPDNTYDFIFMDSAKSKYIVFLPEVL
                     KKVKVGGLIILDDIFQGGDVAKDIKDIRRGQRTIYKGLQRLFDATLDNPDVTASLVSM
                     SDGLLMLRKNVENVDLKHIEI"
     gene            3802..4803
                     /gene="prsA"
                     /locus_tag="ACHU66_001658"
     CDS             3802..4803
                     /gene="prsA"
                     /locus_tag="ACHU66_001658"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HFR3389926.1"
                     /translation="MKQTKKILAGAVTLFAAVTLAACSNAADKDIITMKGNTITVSEF
                     YEKVKTNSQAQQVLLSMVISNVFENQYGDKVSAEEVNKEYDKKAEQLGASFNAALSSA
                     GLTEESYKEQIRTNKLVEYAVKQAAEKELTDENYKAAYDAYTPEVTARVIKLADEAKA
                     KEVLAAAQAEGADFAQLAKDNSTDTTTKDNGGEVKFDSTSTTVPAEVQKAVFALDAGQ
                     VGASVISSVDMKTYTTSYYVVKLDAKSEKSAKWEDYKDKLKEIILAQKQRDSSFVATV
                     LKEALQKANVKVKDSAFQNLLSQYVTTEESSSSSTKSSSSSTESSSSTTESSSSSGQ"
     gene            5068..5553
                     /locus_tag="ACHU66_001659"
     CDS             5068..5553
                     /locus_tag="ACHU66_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HFR3389927.1"
                     /translation="MKQFQVKQRFTFGGEKFDIRDSFGNLAYQVEGSFLEIPKRFIVT
                     NSRGGEVCQITKKFLTFLPQFTIDMVEGHSFYLQKEFTFFKDRYTVENLGLILDGNIW
                     DLDFRLKRPDGMLVAEISKELFHLTSHYSVTVLEDSYADLVISLVVAIDYIEMMEDAS
                     N"
     gene            5833..8451
                     /gene="alaS"
                     /locus_tag="ACHU66_001660"
     CDS             5833..8451
                     /gene="alaS"
                     /locus_tag="ACHU66_001660"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR3389928.1"
                     /translation="MKNLSSAQIRQMWLDFWKSKGHSVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPDNPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPEWFAFPKDKLYMTYYPDDMDSYNRWIALGVEPSHLIPLEDNFWEI
                     GAGPSGPDTEIFFDRGTDFDPENIGIRLLEEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAIFQGAKTNFETDLFLPIIREVEKISGKTYDQDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRASMHGQRLGITEPFLYKLVETV
                     GNIMESYYPEVLEKRAFIEKIVKSEEESFARTIHTGSQFAEQLMDKLAAEGKSEIDGR
                     DIFKLYDTYGFQVELTEELAEHRGMTLDIAGFEAAMKEQQDRARASVVKGGSMGMQNE
                     TLAAITEESTFVYGQTALEASLSVIVADNARTEAVSEGQALLVFDQTPFYAEMGGQVA
                     DHGFVKNATGDIVATVIDVQKAPNGQPLHTVELSASISVGQTYTLEIETKRRKGVEKN
                     HTATHLLHAALHNIIGEHATQAGSLNEQDFLRFDFTHFEAVTAEELRRIEEEVNEQIW
                     NAIPVVTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSVELCGGTHVGNTAEIGI
                     FKILKEEGIGSGTRRIIAVTGREAFLAYRDQEDALKEVAATIKSPQIKEVPNKVESLA
                     QQVRDLQKENAALKEKAAAAAAGDVFKDVKDANGIRYIASQVQVSDAGALRTFADNWK
                     QKDYSDVLVLVAAIGDKVNVLVASKSSDVHAGNLIKVLAPIVAGRGGGKPDMAMAGGS
                     DSSAIQTLLNSVAENL"
     gene            8526..8978
                     /locus_tag="ACHU66_001661"
     CDS             8526..8978
                     /locus_tag="ACHU66_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR3389929.1"
                     /translation="MEHIKDIWQEMYEKAKGHYAPHYVSPFIYSNHVVAAIEAEDGQI
                     YTGYCFEATAGVFHLCAERAAAFNMFQDSGQTTIKRIIAFRNQAPSGEGSGMPCGACR
                     EFLMQLSAENAQTEIMVDFDKRETITLGELMPYWWGEERMQAGTDHLE"
BASE COUNT         2706 a         1723 c         1992 g         2594 t
ORIGIN      
        1 ttgttagcct agtgacgact tgcgatagcg aataccaaaa aaactatttt ccagttgaaa
       61 tagtaaccta taagtcctca ctttccgttg gaaagagggg attttttatg ttagaataaa
      121 gaatacggag gattaattat gtctaaacaa cgttacgaaa ttgaagaaaa ataccagtgg
      181 gatttgacta ctattttccc aaccgatgat gcctgggaag cagaacttgc ggatttacaa
      241 gctgaaactg aaaaaacaaa agaatttgca ggtcatttac tggactcagc taaaagtctc
      301 ttagaaatta gcgaaacgca acttagtctt atgcgtcgca ttgaaaaact ctatgtctat
      361 gcgtctatga agaacgacca agatactcgc gaaggaaaat atcaggaatt tcaagctaag
      421 gccatgggct tgtatgctgc cttttcacag gcttttgcct tctatgagcc tgaatttatg
      481 gccattactg aagagcagct ggaggccttt aaggcagaag agcctactct tgttcagtac
      541 agccaccaat ttgaaaaact tctggcagcc aaggaccatg tcctgtcaca agaggtggaa
      601 gaagtccttg cggcaaccag cgagattttt gaatcacctg ctgaaacctt ctcagtcttg
      661 gacaatgcta gcctgcgttt cccagaaatc gcagatgaag acggtcactt ggtgccactt
      721 tcacacggaa actacatcca ctttatggag tctaaaaacc gtgatgtccg ccaagaagca
      781 tacgaagcta tgtacggtac ctacgagcag ttccagcata catacgctaa gacccttcag
      841 tctaatgtca aagtcaataa cctgaaagct cgtttacgga agtatgactc agctcgccac
      901 gcatcacttg cagctaattt cattccagaa tctgtttacg atacattagt ggcggcagtc
      961 aacaagcacc ttccgctctt gcaccgttat atcaatttgc gtaagaaact cctaggtatt
     1021 gatgacttga agatgtacga tatgtacaca ccattgtcag atttcagcac caagatttct
     1081 tatgaagatg cactcaagaa atgccaagaa accttagaaa tctttggtga ggattattcg
     1141 gctattgtga aggaagcctt cgaaaatcgc tggattgatg tccatgttaa tgaaggaaaa
     1201 cgctcaggag catattcagg aggtgcctac gacaccaacg ccttcatgct tctcaactgg
     1261 caggataatt tggataacat gtacaccctt atccatgaaa cagggcattc tcttcattcc
     1321 atgttgactc gtaagactca accctatgtc tatggtcatt attccctctt cttggcagaa
     1381 attgcttcaa caaccaatga aaatctccta acagaaaaac tcttggcaga agtagaagat
     1441 gacaaggaac gctttgctat tcttaaccat tacctagatg gtttccgtgg cacagtcttc
     1501 cgccagacac aatttgccga gtttgaacaa gctatctaca aggcagacca ggaaggtcaa
     1561 gtcttgacgg ctgatttcct caacgaattg tacggtcaga caaacgagaa atactacgga
     1621 ctttctgccg aagaaaatcc agaaatccag tttgagtggg ctcgtattcc acacttctat
     1681 tacaatttct atgtctatca atatgcgaca ggttttgcgg ctgcatcagc tcttgcaaac
     1741 aagattgtca acggcagtcc agaagacaaa gaaaactatc tcaactactt gaaggctggt
     1801 agctcagatt attccctcaa tgtcattgct aaagcaggtg tagacatgac caaggaagat
     1861 tacctcaacg acgcctttaa ggtttttgag gctcgtttgg aagaattaga agctctcgtt
     1921 gagaaaggtg tgcacctcta atatgatttc ctataaacgt tcgattgtga ttcccagtgt
     1981 gattagtctt gttttcatct tgctcggtct tggtggtttg actagacctg agccatctat
     2041 gggaagtgtt gtctatttga tatttttaca agctttgcta gtttatttat atatattgag
     2101 aaagcaaaag gcagttttta tcttgacaaa acagtaccta gaatttcaag aacacttttg
     2161 gagtgaacgt caacgccatt cgttagaaga gattgcagag attcgataca tcgtttctcc
     2221 tgtctatcgt ggctatcagt cgaaacgttt gcgaattgta ggaaataagg ggggactact
     2281 tgcagtcgtc agcttgaata ggcttgatgg tgcagatttt aatgatattt accattttgt
     2341 agagcaagta gcaccgcata taagatggga tttttcaaat taataaagag aggggactat
     2401 gttaacagaa gaacaactac aagatatttt tgaattagca gatttaatgt caaatggtga
     2461 tagggaacta tttctccaac ttagagaagt agtcttcgcc agtgatccac acgagattct
     2521 caattttatg gaacgtattt tagattcaga gtcattcgat gatttcttgg atcgtgttgg
     2581 agagtctgaa aaggaaaatc tttggttaat cttgataaaa ttactagagc atttgaacta
     2641 tatttgtgtg cgtgattata aggacaatct tgaagatttt atctactttt ttgattgcct
     2701 tcagcaggtc cgcaatgcgg gaatttctct taaattggat tcaggtggat taagtccaat
     2761 tgcatctatt tcagaatggg caagagtcat tgataacaaa tatcttgatg aaaacttttg
     2821 tctaggggca gtagatatgg acacggatag ttactatctc tttttcagta agcaggcgac
     2881 ttttattcga cttcaagaac tagctggaaa tttagggtat cgaattgatt acgccaaaaa
     2941 tatgtaaaga aaaggtgggg aaacctacct tttagtgtgt tgaggaagaa aatcttgtcg
     3001 gcttatgata taatggattc tatggttgaa tcatatagta aaaacgcaaa tcacaatatg
     3061 cgccgtccgg ttgtcaagga agaaattgtg gattttatgc gaacacgtca ggcgcaaaat
     3121 acagggtttt tgaaagaact ggaagagttt gctcatcaag aaaacattcc agttatccct
     3181 catgaaacgg tagcttattt ccgcttgctg atgcagacac tccaacctaa acgtatttta
     3241 gaagttggaa ctgctattgg cttctctgcg ttactgatgg ctgacaatag cccgcactcg
     3301 aagattacga cgattgatcg aaatgaagaa atgattggat ttgctaagga aaactttgcc
     3361 aagtatgatt atcgccagca gattgaattg ctggaaggag aagctatgga tattcttcct
     3421 actttaccag ataacacata tgattttatt ttcatggatt ctgctaaatc aaaatatatt
     3481 gtatttttac cagaagtctt gaaaaaggtc aaagtaggtg gtttaatcat tttggatgac
     3541 atcttccaag gaggagatgt tgccaaggac atcaaggata tcagaagagg gcagcgaacg
     3601 atttataagg gattgcaacg tttattcgat gcgactttgg ataatccaga tgtgactgct
     3661 agtcttgttt ccatgagtga cggtctcctt atgttgagga aaaatgtaga gaatgttgac
     3721 ttaaaacata tagaaattta agaagttttt aagcttttgt gttataataa aggggttaat
     3781 gaaaaaagga gagagtcttt aatgaaacaa actaaaaaaa ttctcgcagg tgcagtaaca
     3841 ctctttgctg cagtaacact cgctgcttgt tccaatgcag ctgataaaga tatcattacg
     3901 atgaaaggta acacgattac tgtgtctgag ttttatgaga aagtaaaaac aaactcacaa
     3961 gctcaacaag ttttgctgtc aatggttatc agtaatgtat ttgaaaatca gtatggtgac
     4021 aaagtctctg cagaagaagt aaataaggaa tatgataaga aggcagaaca acttggtgct
     4081 tcttttaacg cggcactttc ttcggcagga ttgacagaag aatcctataa ggaacaaatt
     4141 cgtaccaata aattggttga gtatgctgta aaacaagctg ctgagaaaga attgacagat
     4201 gaaaactaca aggcagctta tgacgcctat acacctgaag taacagcacg tgtcatcaag
     4261 ttggcagacg aagctaaagc aaaagaagtt ttagctgctg cacaagctga aggtgcagat
     4321 tttgctcaat tggcgaaaga caattccacg gatactacta caaaagacaa tggaggcgaa
     4381 gttaaattcg attcaacttc aacaactgtt ccagcagagg tacagaaagc ggtatttgcc
     4441 ttggatgcag gtcaagtcgg agcgtcggtg atttcttcgg tagatatgaa gacgtataca
     4501 acgagttatt acgttgtaaa attggatgct aaatcagaaa aatctgctaa atgggaagat
     4561 tataaagata agctgaaaga aattattttg gcacaaaagc aaagagattc atcatttgtt
     4621 gctactgtgc tgaaagaagc actccaaaaa gcaaacgtta aggttaaaga cagtgctttc
     4681 caaaatcttc tttcacagta tgtgacaaca gaagaaagca gttcttcctc aacgaagagc
     4741 agctcttcgt cgactgaaag ttcttcttct acaacagaat cttctagttc atcaggtcag
     4801 taattgacga tttacttatt aagaattggt acttgacacc ttagttcaga aaattggcgg
     4861 ttgttgcgag ccatctaagc caagtatttt gctacttaat attacaattg aatagtagag
     4921 cctgtattca cagtttgtgg cttatatata aaagtttttt gcttgtggat acagtttctt
     4981 aaatgagaat gacaagtcat tgaatgaagg tggtaccgcg gtttttcgcc cttcgtttga
     5041 tttcttgtta tttttttgga ggttatcatg aagcaatttc aagtgaaaca gcgctttacc
     5101 tttggtggtg aaaaatttga tattcgtgat agttttggga atcttgctta tcaagtggag
     5161 ggatcttttc tcgaaattcc aaaacgattt atcgtaacca attccagagg tggggaagtt
     5221 tgtcagatta ctaagaagtt tttgaccttc ctgccccagt ttaccattga tatggtagag
     5281 ggacattctt tctacttgca aaaggaattt acttttttca aggatcgtta tactgtggaa
     5341 aatctaggtt taatcctaga cggaaatatc tgggatttgg attttcgctt gaaaaggcca
     5401 gatggtatgc tcgtagctga aatctccaaa gaactctttc atttaacttc tcattattcc
     5461 gttactgtat tggaggactc ctatgcagat ttggtcattt ccttggtggt cgcaattgac
     5521 tacatcgaaa tgatggaaga tgcatcgaat taatcgtgaa ataatcattc agtttaactg
     5581 ttccgttaaa ttgaacccgc ctaactatca cttatgaaag gaaagggtat cgagtttatt
     5641 ctgaaatgga ctcgggctac ggactttccc ctcaattatt actatatgaa aggaaatgtt
     5701 taatttaact gtttcgttaa attgaacccg ctcggaaggc tcggaaaaaa gataaactgc
     5761 ctaggaaatc aagatttcct gtcagtttcc tatttttcag tcgccttcta atcgggcttg
     5821 gtatcttagt ttatgaaaaa tttatcttct gctcaaattc gtcaaatgtg gttggatttc
     5881 tggaaatcaa agggtcactc tgttgagcct tcagcaaatc ttgtccctgt aaacgatccc
     5941 actcttttgt ggatcaactc tggtgtggca accctcaaga aatactttga tggctctgtc
     6001 attccagata acccacggat tacaaatgcc caaaaatcaa tccgtaccaa cgacatcgag
     6061 aacgttggta agacagcccg ccaccacacc atgtttgaaa tgcttggtaa cttctctatt
     6121 ggtgattatt tccgtgatga agcgattgaa tggggctttg agctcttgac aagcccagaa
     6181 tggtttgctt ttccaaaaga caagctctac atgacttact atcctgatga tatggattca
     6241 tacaatcgtt ggattgccct tggtgttgag ccaagccact tgattccgct tgaagataac
     6301 ttctgggaaa tcggtgcagg tccttctggt ccagatacgg agattttctt tgaccgtggt
     6361 actgactttg accctgaaaa cattggtatc cgtctcttgg aagaagatat tgaaaatgac
     6421 cgttacattg agatttggaa tatcgtattg tcccagttca acgcagaccc agctgttcct
     6481 cgctctgagt acaaggaatt gccaaacaaa aacatcgata cgggcgcagg tttggagcgt
     6541 ttagcagcta ttttccaagg ggctaagacc aactttgaaa cggatctctt cttgccaatc
     6601 attcgtgaag tagaaaagat ttctggtaag acctatgacc aagatggcga caacatgagc
     6661 ttcaaggtta ttgcagacca catccgtgcc ctttcttttg ctattggtga cggtgccctt
     6721 cctggaaatg aaggtcgtgg ttatgtcctt cgtcgtctcc ttcgtcgtgc ttctatgcat
     6781 ggtcaacgtt tgggcattac agagccattc ttgtacaaat tggtggagac agttggtaat
     6841 atcatggaaa gttactatcc agaagtactt gaaaaacgtg ccttcattga gaagattgtt
     6901 aagagtgaag aagagtcatt tgctcgtact atccatacag gttcacaatt tgctgagcaa
     6961 ttgatggata aattggcagc agaaggtaag tctgagattg atggtcgtga cattttcaaa
     7021 ctctatgata cctatggttt ccaagtagaa ttgacagaag aattggctga gcaccgtggt
     7081 atgacccttg acattgctgg ttttgaagcg gctatgaaag agcaacaaga ccgtgcccgt
     7141 gcttcagttg tcaaaggtgg ttcaatgggg atgcaaaatg aaaccctggc tgctatcaca
     7201 gaagagtcga cctttgttta tgggcaaaca gctcttgaag caagcttatc tgtcattgtg
     7261 gctgataatg cgcgtacaga agcagtctca gaaggtcaag ccctccttgt ctttgaccaa
     7321 acaccattct atgcagaaat gggtggtcag gttgcagacc atggttttgt gaaaaatgct
     7381 actggagaca ttgttgcgac tgtcatcgat gtgcagaaag caccgaatgg tcagccattg
     7441 cacacagttg aactgtccgc aagtatttca gttggtcaaa cctacacact tgaaatcgaa
     7501 acaaaacgcc gtaagggtgt tgagaagaac cacacagcaa ctcacttgct ccatgcagct
     7561 cttcacaata ttatcggtga gcatgcaaca caggctggtt ccttgaacga gcaagatttc
     7621 ctccgctttg actttacgca ctttgaggca gttactgcgg aagaacttcg tcgtattgaa
     7681 gaagaagtca atgagcaaat ttggaatgct atcccagttg tgacagttga gacagatatt
     7741 gacacagcca aggaaatggg tgccatggcc ctcttcggtg aaaaatacgg taaagaagtc
     7801 cgcgttgtta ctatcggtga ctactctgtg gaactctgtg gtggtaccca cgttggcaac
     7861 accgctgaaa tcggtatctt caagattttg aaagaagaag gtatcggttc aggtactcgc
     7921 cgtatcattg cggtaacagg ccgtgaagcc ttccttgcct atcgtgacca agaagatgcc
     7981 ctcaaagaag tagcggcaac catcaagtcg ccgcagatca aggaagtgcc aaacaaggtt
     8041 gaaagtcttg ctcagcaagt tcgtgacctg caaaaagaaa atgcagctct gaaagaaaaa
     8101 gcagcggcag ccgctgctgg tgatgtcttc aaagatgtca aagatgctaa cggcattcgc
     8161 tacattgcta gccaagtcca agtttcagac gcaggtgccc tccgtacctt cgctgacaac
     8221 tggaagcaaa aagactactc tgatgtcttg gtgttggttg cggccatcgg cgacaaggtc
     8281 aatgtccttg tagcaagcaa gtcaagcgat gttcatgcag gtaacctcat caaagtcctt
     8341 gcgccaatcg tagcaggtcg tggtggtggt aagccggaca tggccatggc cggtggtagc
     8401 gattcctctg ctatccaaac cctcttgaat agtgtagctg aaaacttgta atatagtcag
     8461 tgtatatccc tttgttaaag caactagcaa ggggatatct ttctattcta aaatgaggag
     8521 tacgtatgga acacatcaaa gatatttggc aagagatgta tgagaaagca aaaggtcatt
     8581 atgcgccaca ttatgtttcg cctttcattt attcaaacca tgttgttgct gcaattgagg
     8641 cagaagatgg acaaatttac accggttatt gctttgaggc aacagcaggt gtatttcatc
     8701 tttgtgcaga gagagccgct gcctttaata tgtttcagga tagtgggcag acgacgataa
     8761 aacgcattat tgcctttcga aatcaagctc cctctggaga gggttcagga atgccttgtg
     8821 gagcttgccg tgaatttttg atgcaactgt cggctgagaa tgcacagaca gaaatcatgg
     8881 ttgattttga caaacgagaa accatcacat taggcgaact catgccttac tggtggggtg
     8941 aagaaagaat gcaagctggt acagatcact tggaataata aatgaactct tttatcctct
     9001 ggataaagga gtttt
//
LOCUS       DAZQCJ010000082         8888 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000082 DAZQCJ010000000
VERSION     DAZQCJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8888
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.083"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            56..1603
                     /gene="cls"
                     /locus_tag="ACHU66_001662"
     CDS             56..1603
                     /gene="cls"
                     /locus_tag="ACHU66_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR3389930.1"
                     /translation="MEKLWKIIYSRTFIVISLIILTIFLILWLVGSAATYMPALLIIL
                     QIFSIFVAISIINRPMNTSFKLTWIIFVIGIPIFGALFYFILQSNIETKRYRKNFHKQ
                     AQRLREYSKTSEKVMKALEQEDKEQLKLAHFMSEYVGYPIHTNTDAVYFSTGQEKFEA
                     LLEELKKAEQYIFMEYFIVDMGYMWDSILDILKEKAAQGVEVRFMYDGMNSLTNLPYN
                     YYKTLRKYGIKAKVFSQIIPALSTVQNNRDHRKIAIIDGKVAFTGGINIADEYINKKV
                     RFGYWKDAAIMIRGEAVANFTLMFLQMWNYNEKTETDDLKYLKTHKDLETETVDAEGF
                     FLPYGENPFDGDEVAKRVCLDMIQSATDYIYIMTPYLVLDDEMIDNLTYAAKRGVEVR
                     ILLPHIYDKKSAYLAARTDFPTYLEAGIEIFEFQPGFVHSKVVLVDDKKATVGTVNMD
                     FRSFYLNFECGLYIYNHSAVLAAIKDDFTHSFKKSQQINLLTFHKTYSWYKRLGGALL
                     RIISPLL"
     gene            1759..3972
                     /gene="recJ"
                     /locus_tag="ACHU66_001663"
     CDS             1759..3972
                     /gene="recJ"
                     /locus_tag="ACHU66_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR3389931.1"
                     /translation="MIKPNYDWQLLTGFSDEQFIKIAKKEGVDPVAAKLLYERGIHSA
                     EELHTFLQPSLEDLHDPYLLHDMDKAVERIRRAIEDYEQILIYGDYDADGMTSASILK
                     ETLEEMGAEVQVYLPNRFTDGYGPNQSVYKYFIEQQGISLIVTVDNGVAGHEAIAYAQ
                     EMGVDVVVTDHHSMQETLPNAYAIVHPEHPEGNYPFKHLAGCGVAFKLACALLETVHA
                     DLLDLVAIGTIADMVSLTDENRVMVKYGLSLLKQTERAGLQELIKIAGIDIDSIDEET
                     VGFQLAPRLNALGRLDDPNPAIELLTGFDDEEAHQIALMIDSKNVERKDVVQAIYDEA
                     KTMLRQDRPVQVLAKEGWNPGVLGIVAGRLLEELQQPVIVLSIEDGKAKGSARSVEAV
                     DIFKALKDHQDLFIAFGGHAGAAGMTLEVDKLEELALTLTDYIIENKLDLSSKSSLVL
                     DEELDLEELTLDTLKSFEKLAPYGRDNKKPVFYIRDFQVESARTMGQNNAHLKLRISK
                     GAAGFDVVAFGKGNLALEFSQAKGLELAVTLSVNQWNGNTSLQLMLVDARVNGVQLYN
                     IRGKQHPIPAGVPILDIENPVADNKAIVLANLPEDLSSLRSYFQEREFEAIYFKNEIA
                     KPYYLDGYGSREQFAKLYKTIYQFDEFDVRYKLKDLAVYLKIKDSLLVKMIQIFQELE
                     FVTITDGVMRVNKDAQKREISESQIYQELKETVIQQELMALGTVQEIYEWLCGRV"
     gene            3951..5081
                     /locus_tag="ACHU66_001664"
     CDS             3951..5081
                     /locus_tag="ACHU66_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HFR3389932.1"
                     /translation="MAMWSRLMTVEALVVVGVIGTLLYTYWFIRQKSLLKNLEQITEI
                     KHALIELRRVGWSERAIKKVFLKQLLPLKQEDIPAFVQNFIKETTIFASTSFYQLIRV
                     DSRSLSQEKATALLEQSMNMLDFPEELSHGILPELLQKMDVNCPPHHPFWRYFAKLVD
                     KVFPVRELEVKRPLNRQVHQLRYFISYQQAFLVRQQFGKEKTDWQALIAYLRSLPRWF
                     YRLRESARLHNKQLFGKKNQKTLPVNMKILIHFHSEFILNQDGQFALILEERPHVNGV
                     VNGASFNYARANNKRHRQLDMAPVGRQDPVFRKELLRSKMGVYLSPTRFRRYQKGRNE
                     VGWEQSYFNQQGSFSYGGHSRAACVANLRRRFAKDIGLKCTK"
     gene            5135..5647
                     /locus_tag="ACHU66_001665"
     CDS             5135..5647
                     /locus_tag="ACHU66_001665"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR3389933.1"
                     /translation="MNLKDYIATIPNYPKEGIEFRDISPLMADGNAYSYAVREIVQYA
                     TDKQIDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLIELDDLKGREAIG
                     DYDYKVLMHY"
     gene            5751..6692
                     /gene="metA"
                     /locus_tag="ACHU66_001666"
     CDS             5751..6692
                     /gene="metA"
                     /locus_tag="ACHU66_001666"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR3389934.1"
                     /translation="MPIKIEKSLPAVEILRKENIFVMDSDRASHQDIRPIKILILNLM
                     PQKIVTETQLLRLLANTPLQLEVEFLYMASHESKNTHSDHLKQFYKTFEQVRDSYFDG
                     LIVTGAPVENLPFEEVDYWQELTQVFDWSKTHVYSTLHICWGAQAGLHARYGVPKHSM
                     SRKLSGVYCQEVTNPTSPLMRGFDDEFRSPHSRYTEVREADIAHLSGLTILSQGPEVG
                     LSILASKDLREIYSFGHLEYDRDTLAKEYQRDCIAGKNPHLPENYFKEDDPTSKPSLS
                     WNLPAAQFFTNWVNYAVYQETPYDWHIFEQSAQSASL"
     gene            6702..7376
                     /locus_tag="ACHU66_001667"
     CDS             6702..7376
                     /locus_tag="ACHU66_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HFR3389935.1"
                     /translation="MNYSQQFRQGHLVLPAAILFHYQELFPSADDFLIWQFFLYQNSS
                     AIESLAPSEIAQATGKTVAQVNQAIENLQDAGLLEFKTISIAGEIEMIFDALPAFEKL
                     DVLLTPKQAVEIVQPENDLKTLVGDFERELGRFLSPFEIEDLQKTIEDDKTSIDLVRA
                     ALKEAVFNNKTNWKYIQAILRNWRREGITTVAQVEAKNAEREIQTPKNVTVSSDFLDA
                     MDLWKD"
     gene            7378..8061
                     /locus_tag="ACHU66_001668"
     CDS             7378..8061
                     /locus_tag="ACHU66_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFR3389936.1"
                     /translation="METKLSKRLETVASFVPQGARLVDVGSDHAYLPLFLVEQGRIDF
                     AIAGEVVQGPYQSALQNVEQAGQTEKILVRLANGLGAFEKSDQMTAVTIAGMGGRLIA
                     EILEAGKEKLATIERLILQPNNREDDVRRWLEKNGFRLVAEKILEENGKLYEVLVAES
                     GQMRLSEVEHRFGPYLLEEASPVFVKKWRRELEKLSFALEQVPSERQGDRFAISQKIQ
                     QIQEVVHVS"
     gene            8051..8845
                     /locus_tag="ACHU66_001669"
     CDS             8051..8845
                     /locus_tag="ACHU66_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFR3389937.1"
                     /translation="MLASQVIERYQAFCPPSLAMEGDVKGLQIGTLNKEIQRVMVALD
                     IRETTVAEAIDKKVDLIIVKHAPIFRPINDLVADNPQTKMYLDLIKHDIAVYVSHTDI
                     DVVEGGLNDWFCELLEIQDTTYLIETAEGQGLGRVGTIAEQTLEELALKVKSVFGLDA
                     VRLVTYGHQAKQYVSRVAICGGSGGSYYHEALAKGADVYITGDIYYHTGQDMLTQGLL
                     AIDPGHHIEALFIKKIAELLEIWKAKEGWDVEILPSTVSTNPFRHL"
BASE COUNT         2669 a         1595 c         2031 g         2593 t
ORIGIN      
        1 gagtaggata atgaactaaa atctgttata atggtaaaaa ataagggaga gatgtatgga
       61 aaaactttgg aaaattatat atagtcggac atttattgtc attagtttga ttattctaac
      121 cattttcctt attctttggt tggtaggttc ggcagctacc tatatgcctg ctttattgat
      181 tattttgcaa attttttcga tttttgtggc tatttcaatc atcaatagac cgatgaatac
      241 cagttttaag ttaacctgga ttatttttgt cattggtatt ccgatttttg gtgctctatt
      301 ctattttatt ttgcagtcca acattgaaac caaacgttat cgtaaaaatt ttcataagca
      361 agcacaaaga cttcgggagt atagtaagac gtctgaaaag gtcatgaagg cattggagca
      421 ggaagataag gagcaactga aattagctca cttcatgagt gagtacgttg gctacccaat
      481 tcataccaat acggatgccg tctatttttc aacaggtcag gaaaaatttg aagctctact
      541 agaagaattg aaaaaggcag agcagtatat tttcatggag tactttattg tcgatatggg
      601 atatatgtgg gattctatcc ttgacatttt gaaggaaaag gcagcgcaag gggtggaagt
      661 tcgttttatg tatgatggga tgaattcact gaccaatctt ccctataatt actataaaac
      721 cttgcgaaaa tatgggataa aagccaaagt attttctcaa attatcccag ccttatctac
      781 ggtgcaaaat aaccgtgacc atagaaaaat tgcgattatt gatggaaagg ttgcctttac
      841 cggtggcatt aatatcgcag atgaatacat caacaagaaa gtgcgttttg gttactggaa
      901 agacgcagcg attatgatta ggggagaagc tgttgcaaac tttacgttga tgttccttca
      961 aatgtggaat tacaatgaaa agacagagac agatgaccta aaatacctca aaacgcataa
     1021 agatttagag acggaaaccg ttgatgccga aggtttcttc cttccgtatg gcgagaatcc
     1081 atttgatgga gatgaggtag ccaaacgagt ctgcctagac atgattcagt cggcaacgga
     1141 ttatatttat atcatgacac cctacctagt gttggatgat gaaatgattg ataatttgac
     1201 ttatgcagcg aagcgtggag tcgaggttcg cattctacta ccgcatatct acgataaaaa
     1261 atcagcctat ttggctgctc gcactgattt tccgacttac ttggaagcag gaattgaaat
     1321 ctttgaattc cagcctggat ttgtccattc caaagttgtt ttggtcgatg ataagaaagc
     1381 tactgttggt acggtgaata tggattttcg ctctttttat cttaattttg aatgcggtct
     1441 ctatatttac aatcactcgg cagttttggc agctattaag gatgacttta ctcattcctt
     1501 caaaaaatca cagcaaatta accttctaac cttccataaa acgtactctt ggtataagcg
     1561 tttgggagga gccctactac gaattatttc gccgttatta taaattgcaa tattcgttct
     1621 tcatggagca agggatcgac attctgtttc ggtcttacct tgctccattt ttcttggaaa
     1681 atgatataat agaaagatag tttatactcg gaaaatttac aggctacttg tgattttgag
     1741 tattaaggag taaaaaacgt gataaaaccc aattatgact ggcaattatt gaccggtttt
     1801 tctgatgaac aatttatcaa aattgctaaa aaagaaggag tggacccggt agcggctaag
     1861 ttgctgtatg aacgtggcat ccattcggcg gaagaattgc atactttctt acaaccaagc
     1921 ttagaagatc ttcacgatcc ctatttacta catgatatgg acaaggcggt agagcggatt
     1981 cgccgagcaa tcgaagatta cgaacaaatt ttgatttatg gagattatga cgcagacggc
     2041 atgacctcgg catctattct gaaagaaact ttggaggaaa tgggggcaga agtccaagtt
     2101 tatcttccca accgtttcac agatggctat gggccgaatc agtcagttta caagtatttt
     2161 atcgagcaac aggggatttc tcttattgtg acggtggaca atggcgtagc gggtcatgaa
     2221 gcgattgcct atgcccagga aatgggtgtc gatgttgtcg tgacagacca ccattccatg
     2281 caggaaaccc ttcccaatgc ctatgcgatt gtacatccag agcatccaga gggaaattat
     2341 cccttcaaac atttggcggg ctgcggtgtg gcttttaaat tggcctgtgc tcttcttgaa
     2401 actgttcatg cagacttgct ggatttggtc gcaattggca ccatcgctga tatggttagc
     2461 ttgaccgatg agaatcgtgt catggtcaaa tatggtctct cgcttctcaa acagactgag
     2521 cgagcaggtt tgcaagagtt gataaagata gctggtattg acatagacag tattgatgaa
     2581 gaaacagttg gtttccaact tgctccgagg ttgaatgccc ttggtcgact cgacgatcct
     2641 aatccagcca tcgaattact gactggtttt gatgacgaag aggcccatca aattgctctc
     2701 atgattgaca gtaaaaatgt agaacgcaaa gacgtcgtcc aagcaattta cgatgaagca
     2761 aaaaccatgc tccgtcagga tagaccggtt caagttctgg ctaaagaagg ttggaaccca
     2821 ggtgttttag ggattgtagc aggacgccta ctagaagaat tgcagcaacc tgttatcgtc
     2881 ttgtctattg aagatggtaa ggcaaagggg tcggctcgtt cggttgaagc agttgacatt
     2941 ttcaaggctc ttaaagatca tcaggaccta tttatcgcct ttggtggtca tgctggtgca
     3001 gcaggtatga ccttggaagt tgacaaactt gaagaacttg ctctgacctt gacagactac
     3061 attatcgaaa ataaattgga tttatccagc aagtcttcgc tggtcttaga tgaagaattg
     3121 gatttagagg aattaaccct agatactcta aaatctttcg agaagcttgc gccttacggc
     3181 agggataata aaaagcctgt attctacatc agagactttc aggtggaatc agcaaggacc
     3241 atggggcaga ataatgccca tttgaaacta cgcattagta aaggtgcggc aggttttgat
     3301 gtggttgctt ttggtaaggg caatctagcc ttggaatttt cacaggccaa gggcttagaa
     3361 ctagctgtca ccttgtctgt caatcaatgg aatggcaata ccagtcttca gctcatgttg
     3421 gttgatgctc gggtcaatgg tgttcaactt tataatattc gtggaaagca acatcctatt
     3481 cctgctggag tcccaatttt ggatattgaa aatccagtag cagataacaa agcaattgtc
     3541 ttggctaact tgcctgagga cctcagtagt ctgcgtagct attttcagga aagagaattt
     3601 gaagccattt attttaaaaa cgaaattgcc aaaccgtact atctagatgg ttatggtagc
     3661 cgagaacaat tcgctaaatt gtataagact atttaccaat ttgatgagtt tgatgttcgc
     3721 tacaaactga aagatttggc tgtttactta aaaattaagg acagtctatt ggttaaaatg
     3781 attcagattt tccaagaatt agaatttgtc acgattacgg atggggtcat gcgggtcaac
     3841 aaagatgcac agaagcgaga gatttcagaa agtcaaatat atcaagaatt gaaagaaaca
     3901 gttatccaac aagagttgat ggcgcttggc accgttcagg aaatttatga atggctatgt
     3961 ggtcgcgttt gatgacagta gaagctctgg ttgttgttgg agtgattgga acgcttctct
     4021 acacttattg gtttatcagg caaaaaagtc tgttgaaaaa tcttgaacag ataaccgaaa
     4081 taaagcacgc actgattgag ttacggcgag taggatggtc agaaagagcc atcaaaaagg
     4141 ttttcctgaa acaactgttg cctctaaaac aagaagacat cccagcattt gttcaaaatt
     4201 tcataaaaga aactactatt tttgcctcga ccagctttta tcagttgata agagttgaca
     4261 gtcgctcttt aagccaagag aaagcgacgg ctttattgga gcaaagtatg aatatgttag
     4321 attttcctga ggaattaagt cacgggatat tacctgaact tttgcagaag atggatgtga
     4381 attgtcctcc acaccatccg ttttggcggt actttgctaa gctagttgat aaggtttttc
     4441 cagtgcggga attagaagtc aaacggccgt taaacagaca ggtccaccaa ctgcgctact
     4501 ttatttccta tcagcaagct tttttggtac gtcagcagtt tggaaaagaa aaaacagatt
     4561 ggcaggctct gatagcctat ttacgtagcc tacctcgatg gttttaccgc ttacgagaat
     4621 ctgcccgcct acacaataaa caactgttcg gcaagaaaaa tcagaagact ttgccagtca
     4681 atatgaagat tttgattcat tttcacagtg aatttatcct aaatcaagat ggacaatttg
     4741 ccttgatttt agaagaacga cctcatgtca atggcgtggt aaacggtgct tcctttaatt
     4801 atgccagagc taataataag cgtcatagac aacttgatat ggctccagtt ggcaggcagg
     4861 atcccgtttt tcggaaagaa ttgctacgct caaagatggg agtctattta tcgcctactc
     4921 gctttcgtcg ctaccaaaaa ggaagaaatg aagttggctg ggagcagtct tactttaatc
     4981 agcaaggaag cttttcctat ggtgggcata gcagggcagc ttgtgtggcg aatttgagga
     5041 gacgatttgc taaagatata ggtctgaaat gtaccaaata acaatttcat ggtataatga
     5101 agagtaaaaa ttatttttaa aaagagagag acaaatgaac ttaaaagatt acatcgcaac
     5161 aattcctaac tatccaaaag aaggtatcga attccgtgac attagccctt tgatggcaga
     5221 tggaaatgct tatagctatg cagtacgtga aatcgttcag tatgcgacag ataagcaaat
     5281 cgatatgatt gttgggccag aggcgcgtgg tttcattgtt ggatgtccag ttgcctttga
     5341 attgggaatc ggttttgcac cagttcgtaa gccaggtaag ttaccacgtg aggttatctc
     5401 agctgactat gaaaaagaat atggtgtaga tacattgacg atgcatgcgg atgcgattaa
     5461 gccaggtcaa cgtgttctta tcgttgatga cttgttggca actggtggta ctgtaaaagc
     5521 aacgatcgaa atgattgaaa agcttggcgg tatcgttgct ggctgtgcct tcttgattga
     5581 gctagatgat ttgaaaggcc gcgaagcaat cggtgattac gactacaagg ttttgatgca
     5641 ctattaatct aaggttatag ccaaaactta taactggtcc tatttttatt ctaattgaat
     5701 tgcatagcaa cctgtcttat aataaatata agaaaattgg gaggttgctt atgccaatta
     5761 aaattgaaaa atcattacca gcagttgaaa ttttgagaaa agagaatatt tttgtgatgg
     5821 atagtgaccg agccagtcat caggacattc gtcctatcaa aatccttatt ctcaatctca
     5881 tgccacagaa gatagtgacg gagactcagt tgctccgtct tttggctaac actcctttac
     5941 agctagaggt tgaatttctc tacatggcta gtcacgagtc caaaaataca cactcggacc
     6001 atttgaagca gttttataag acttttgagc aggtcaggga tagctatttt gatgggctca
     6061 tcgtgacagg agcaccagtt gaaaatttac cttttgagga agttgattac tggcaagaac
     6121 ttactcaagt ttttgattgg tcaaaaacac atgtttattc aacattgcac atttgttggg
     6181 gtgcacaggc aggtttgcat gcacgctatg gtgtacccaa acacagtatg tctcgaaaac
     6241 tatcaggggt atattgtcaa gaagtgacta atccaacgag ccctcttatg cgtggatttg
     6301 acgatgaatt tcgttcacca cattcccgtt atacggaagt aagagaagca gatattgccc
     6361 atctgagtgg gttgacgatt ttatcacaag gtccagaagt tggtctatcc attctagcca
     6421 gcaaggattt aagagaaatt tatagttttg gacatttaga gtatgatagg gatacactag
     6481 ctaaggaata tcaacgtgat tgtatagctg gaaaaaatcc ccatttaccg gaaaattatt
     6541 tcaaagaaga tgaccctaca agcaaaccat ctctctcttg gaatttacca gcagcacaat
     6601 tctttaccaa ctgggtgaat tatgctgtct atcaagaaac tccatacgac tggcatatct
     6661 ttgaacaatc agcccagtca gctagtcttt aaagaggaag tatgaactat tcacagcaat
     6721 ttcgtcaggg acatttggtc ctgccagcag caatattatt ccactatcaa gagctttttc
     6781 cgtcggcaga tgattttttg atttggcagt ttttcttata tcaaaattct tcagctatcg
     6841 aatcattagc gcctagtgag attgctcaag ctactggtaa aacagttgct caggtcaatc
     6901 aagctattga aaatctccaa gatgctggac tcttagaatt taagacaatc agtattgctg
     6961 gtgaaattga aatgatattc gatgcgcttc ctgcttttga aaaattggat gttctgttaa
     7021 ctccaaaaca ggcagttgag attgtgcaac cagagaatga tttgaagacc ttggtgggtg
     7081 attttgaacg tgaattgggc cgctttttat cgccgtttga aattgaagat ttgcagaaga
     7141 ctattgagga tgacaaaact tcgattgatt tggttcgtgc agccttgaaa gaagctgttt
     7201 ttaacaacaa aacaaattgg aaatacatcc aagctattct acgaaactgg cgccgtgagg
     7261 gaattactac agttgcgcaa gtagaagcga aaaatgcaga aagagaaatc cagactccta
     7321 agaatgtgac tgtttcaagt gactttcttg atgcaatgga tctctggaaa gattgatatg
     7381 gaaacaaaat tatctaaaag gctggaaaca gtcgcaagtt ttgtcccaca aggggcacgt
     7441 ctagttgatg tagggagtga ccatgcttat ctccctcttt ttttggtaga gcaggggcgt
     7501 attgattttg ccattgcggg ggaagtcgtt caaggtccct atcagtcagc gctccagaat
     7561 gtagagcagg cgggacaaac agagaaaatt ctagtccgtt tggcaaatgg cttaggcgcg
     7621 tttgagaagt ctgaccagat gactgccgtg accatagcag gtatgggggg acgcttgatt
     7681 gcggaaatct tagaagcagg aaaagaaaag cttgcgacca ttgaacgttt gatactccag
     7741 ccaaataatc gtgaagatga tgtgcgtcga tggttggaaa aaaatggttt tagattggtt
     7801 gctgaaaaga ttctggaaga aaatggcaag ttatatgaag tcctcgttgc agaatcagga
     7861 cagatgagat tgtcagaagt tgaacatcgt tttggtccct atctactaga ggaggcctca
     7921 ccagttttcg tcaaaaagtg gagaagagaa cttgaaaaat taagttttgc tttggaacaa
     7981 gttccatctg aacgccaagg tgaccgcttt gccatttccc aaaagatcca acaaattcag
     8041 gaggtagtcc atgttagcta gtcaggttat tgagcgctat caagcctttt gtcccccttc
     8101 tttggctatg gaaggcgatg tcaaagggct gcaaatcggt acccttaata aagagattca
     8161 acgtgtcatg gtggctctgg atattcgtga gacaacagtg gcagaggcga ttgataaaaa
     8221 ggtagatttg attatagtca agcacgctcc gatttttcgt ccaataaatg atttggtggc
     8281 tgacaatccg caaactaaaa tgtatcttga tttgattaag catgatattg cagtctatgt
     8341 tagtcataca gatattgatg tggttgaggg tggtctcaac gactggttct gtgaactatt
     8401 agaaattcaa gatactacct acttgattga aactgccgag gggcaggggc ttggccgtgt
     8461 ggggacaata gcagagcaga ctttggaaga attggctttg aaagtcaagt ctgtctttgg
     8521 tctagatgct gtccgtctag tgacctatgg gcaccaagcc aagcagtatg tcagccgtgt
     8581 agccatctgt ggaggcagtg gtgggtctta ctaccatgag gctcttgcta agggggcaga
     8641 tgtttacatc acgggcgata tttactacca tactgggcag gacatgctga ctcagggctt
     8701 gctggctatt gatccaggcc atcatatcga agcattattt atcaagaaaa ttgctgaatt
     8761 gttggaaata tggaaggcaa aagaaggttg ggatgtcgaa attctacctt caactgtttc
     8821 aaccaatccg tttagacatt tgtgataaat taatcagcac ttgattgtgg aaagactaag
     8881 acgacatt
//
LOCUS       DAZQCJ010000083         8683 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000083 DAZQCJ010000000
VERSION     DAZQCJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8683
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.084"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(4..1485)
                     /gene="guaB"
                     /locus_tag="ACHU66_001670"
     CDS             complement(4..1485)
                     /gene="guaB"
                     /locus_tag="ACHU66_001670"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR3389938.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPHDIDLKTQLAPNLTL
                     NLPIISAAMDTVTDSKMAIAMARAGGLGVIHKNMSIAEQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEAEKLMATYRISGVPIVETLENRKLVGIITNRDMRFISDYSQPISTNMT
                     SDALVTAPVGTDLATAEAILHKHRIEKLPLVDENGRLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIKEIREHFPTR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAGVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGHAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKQGSKDRYFQASVNEANKLVPEGIEGRVAYKGSVADMIFQMVGGLRSGMGY
                     VGAGNLTELHENAQFIEMSGAGLKESHPHDVQITNEAPNYSVQ"
     gene            complement(1717..2742)
                     /gene="trpS"
                     /locus_tag="ACHU66_001671"
     CDS             complement(1717..2742)
                     /gene="trpS"
                     /locus_tag="ACHU66_001671"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR3389939.1"
                     /translation="MSKPIILTGDRPTGKLHIGHYVGSLKNRVLLQDAGQHELFVFLA
                     DQQALTDHAKEPQKIVESVGNVALDYLAAGLDPAKTTIFIQSQIPELAELTMYYMNLV
                     SVARLERNPTVKTEIAQKGFGEGIPAGFLVYPVSQAADITAFKANFVPVGNDQKPMIE
                     QTREIVRSFNHAYQTDILVEPEGIYPENEAAGRLPGLDGNAKMSKSLGNGIYLADDMD
                     TLKKKVMSMYTDPNHIRVEDPGKIEGNMVFHYLDVFGREEDKAEIEAMKEHYQRGGLG
                     DVKTKRYLLDILERELGPIRERRIEFAQDMGQVYAMLEAGSQKARQVAATTLDQVKSA
                     MGINYFK"
     misc_binding    complement(2789..2995)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3097..3969
                     /locus_tag="ACHU66_001672"
     CDS             3097..3969
                     /locus_tag="ACHU66_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFR3389940.1"
                     /translation="MKERIKDFISVTLGSIVMAIGFNSFFLENNIVSGGVGGLAIALN
                     ALLGWSPSDFVLYCNIPLLIICWFFLGKSVFIKTVYGAIIYPLCIKLTAGLPNLTENP
                     LLAAIFGGIILGFGLGLVFLGNSSTGGTGILIQFIHKYTPLSLGLTMAIIDGIIVGLG
                     FVAFDTDTVMYSIIALMTITYIVNRMMSGTQSSRNVMIISQKSEEIKDYITKVADRGV
                     TELPIIGGFTGVDKRMLMTTISIPEMQKLETAVLEIDETAFMVVMPASQVRGRGFSLQ
                     KDHKHYDEDILIPM"
     gene            4034..5656
                     /locus_tag="ACHU66_001673"
     CDS             4034..5656
                     /locus_tag="ACHU66_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFR3389941.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPSTGHISLGPDERLSVLRQNHFDYEDERVIDVVIMGNEQLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNISEDLHYQNMSELTNGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSINWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKIFVGNYDFWKQSSELAAKLQADRNAKAEEKIKELQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIIPSSRKYPFINFKSEREIGNDLLTVENLKVVIDGETI
                     LDNISFILRPGDKTALIGQNDIQTTALIRALMGDIEYEGTIKWGVTTSQSYLPKDNTR
                     DFDTNESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKAFKESIIFASHDHEFIQTLANHIIVI
                     SKNGVIDRIDETYDEFLENAEVQAKVQELWKA"
     gene            5963..8542
                     /locus_tag="ACHU66_001674"
     CDS             5963..8542
                     /locus_tag="ACHU66_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFR3389942.1"
                     /translation="MKKIFTKTSIYYLLSFLIPLTIISIVLAFQGIWWGSDTTILASD
                     GFHQYVIFNQTLRNTLHGDGSLFYTFSSGLGLNFYALSSYYLGSFLSPIVFFFDLQSM
                     PDAIYLVTIVKFGLTGLSTYFSLKGIHKNLKEEWALLLATSFSLMSFSTSQLEINNWL
                     DVFILLPLVLLGLHRLLKKQGPILYYITLTCLFIQNYYFGYMVAIFLTLWTLVQLSWI
                     DSQRIKRFIKFTIVSILSALSSMFMLLPTYLDLKTHGETFTKIVNLKTEDSWYLDFFA
                     KNLVGSFDTTKFGSIPMISVGLVPLILALLFFTLKEIKPTIKLSYALFFTFIISSFYL
                     QPLNLFWQGMHAPNMFLYRYAWALSITVIYLAAETLVRLRQVSIKNFTLIVSFLLICF
                     TSTFIFRDHYEFLTDVNFLLTLEFLIAYFILFVAMIRYKSSLKWINIVLLFFTFLELG
                     LHSHYQVQGISDEWHFPSRSNYEEKLTDIDSIVKSTKTTTDSFYRIERLLPQTGNDSM
                     KFNYNGISQFSSIRNRASSSALDKLGFRSDGTNLNLRYQNNTIIADSLFGVKYNLATT
                     DPNKFGFTLNQSQSTINLYENSFNLGLALLTEGIYKDVNFTNLTLDNQTNFLNQLTGL
                     SQKYYHTLSDVISQNTVELSNRMTVNKVDNEDAAKATFLVNIPANSQVYLNLPNLTFS
                     NENQKKVVITVNNQSSEFTLDNAFSFFNVGSFTTDVQVQVDVYFPENNQVSFDKPQFY
                     RLDLLAFQQAISILQEKQVVTKTDGNKVTVDFVTDKEASLLLTLPYDKGWNATIDGKP
                     IKIQKVQEGFMKVDVSPGQTKLVLTFVPNGFYLGLLISFGAVFVFFSYQFIGYYYSKN
                     REY"
     gene            complement(8611..8683)
                     /locus_tag="ACHU66_001675"
     tRNA            complement(8611..8683)
                     /locus_tag="ACHU66_001675"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8648..8650),aa:Asn,seq:gtt)
BASE COUNT         2572 a         1828 c         1540 g         2743 t
ORIGIN      
        1 gttttactga actgaatagt ttggagcttc gtttgtgatt tgaacatcgt gtggatggct
       61 ttctttcaaa ccagcacctg acatttcgat gaattgtgca ttttcatgca attcagtcaa
      121 gttaccagca cctacatagc ccatacctga gcgaagtcca ccaaccattt ggaaaatcat
      181 atctgcaact gaacctttat aggctacacg tccttcaatt ccttctggaa caagtttgtt
      241 ggcttcattg acagatgctt ggaagtaacg gtcttttgaa ccttgtttca tggcagcgat
      301 agagcccata ccgcgatagg tcttgaactt acgaccttgg aagatttctg tctcacctgg
      361 tgcttcatcc gttcctgcaa acatagaacc aagcataacg gcatgaccac ccgctgcaag
      421 tgccttgaca atatctccag aatatttgat accaccatca gcgataatcg ttttaccata
      481 ttcacgcgca acacctgccg catcgtaaat agccgtcacc tgaggaactc ctacacctgc
      541 gattacgcga gtcgtacaga tggaacctgg accaatacca actttaacga catcaacccc
      601 tgcttcataa agggcacgtg caccctctgc agtcgcaatg ttgccggcaa tcaaggtacg
      661 agttgggaag tgttcgcgga tttctttgat cttacgcaaa acaccagctg aatgaccatg
      721 agctgtatcg atgacaatcg catctgcacc tgcttcaaag agagcttctg cacgttcaaa
      781 agtatcagat gtaacaccaa cagcacccgc aaccaagaga cgaccaaatt catccttcgc
      841 agcatttggg aactcaatca ctttctcaat atctttgata gtaatcaagc ctgacaaacg
      901 accattttca tcaaccaatg gcaatttttc aatacgatgc ttgtgaagaa tagcctccgc
      961 agtcgcaaga tctgttccaa ctggcgctgt taccaaagca tcgctggtca tgttggtcga
     1021 aattggctgt gagtaatctg aaatgaaacg catatcgcga ttggtgataa taccgaccaa
     1081 cttacgattt tctaatgttt caacgattgg cacaccggaa atacgataag tagccatcaa
     1141 tttctcagct tcagcaatgg tgtgttctgg tgttaagaag aatggatcga taatgactcc
     1201 attttcagaa cgttttacct tgcgcacctc atcagcctgc tccgcgatag acatgttctt
     1261 atggatgacg cccaaaccac ccgcacgcgc catagcgata gccattttgc tatctgttac
     1321 tgtatccata gcagcagaaa taatcggaag attaagggtc aaatttggtg ctaattgtgt
     1381 ttttaaatca atatcatgtg gtaatacatg actttcagcc ggaataagta atacatcgtc
     1441 gaaagtaaag ccttttttca aaaatttagt gtcccagttt gacattttct tcctcttttc
     1501 ttatttaata tgtagcatga ttgctatttg tattttaatt atgataacat tttgaaatca
     1561 tttgtcaatc attattcgct ttaatatcat cattactaaa aattcagaaa attctattca
     1621 gttttatata taaaattcga acgtttgttt tttgctacgt cgtaaaataa atgaaaaaag
     1681 caacattttc tcaacattgc tctttcattg ttaaccttat ttaaaatagt taattcccat
     1741 agcagatttc acttggtcaa gagttgtcgc agcaacttgg cgagcttttt ggctacctgc
     1801 ctccaacatt gcatagacct gtcccatatc ctgtgcaaat tcgatacgac gctcacggat
     1861 tggtcctaat tctctctcta aaatgtccag caaataacgt ttggtcttca catcgcccaa
     1921 accaccacgt tgatagtgct ccttcatagc ctcaatctca gccttatctt cttcgcgacc
     1981 aaaaacgtca agatagtgga aaaccatgtt tccttcaatc ttacctgggt cttcaacacg
     2041 gatgtggttg ggatcagtgt acatggacat gactttcttc ttgagtgtat ccatgtcatc
     2101 tgccagataa ataccatttc caagtgactt agacatcttg gcattgccgt ccaaacctgg
     2161 caagcgacca gctgcctcat tttcaggata aatgccctcc ggctctacca aaatatcagt
     2221 ctggtaagca tggttaaagc tacgaacgat ttcacgagtc tgctcaatca taggtttctg
     2281 gtcatttcct actggaacaa agtttgcctt gaaggctgta atatccgccg cttgtgacac
     2341 aggatagacc aagaaaccag ctggaattcc ttcgccaaat cccttctgag caatctcggt
     2401 ttttacagtc ggattgcgtt ctagacgtgc aacagatacc aagttcatgt agtacatggt
     2461 caactctgcc aattcaggaa tttgactctg gatgaaaatc gttgtcttag ctggatcaag
     2521 tcctgccgct agataatcta gtgctacgtt tccaacggat tcaacaatct tttgcggttc
     2581 ctttgcatga tctgtcaacg cttgttggtc tgctaaaaag acaaaaagtt catgttgacc
     2641 tgcatcctgt aataaaacac gatttttcaa cgaaccaaca taatgtccaa tatgtaattt
     2701 gcctgttgga cggtctcctg ttaggataat cggtttactc ataaaatgtt ctccttttac
     2761 ataataaaag cccacgcaca gaaaactgtg cgagggcgtc aaggacacgg tgccacctca
     2821 cttataagca ttaaataagc ttacatcttt ttgcacataa ggcctgctag ccgaatcctt
     2881 atttattcag attctattca ctcagtccat tcataagcga ctcttgtttg ttcacaccaa
     2941 ccacaaactc tctaaaaatt atccacttac tactcttctg attgattcct attataaccg
     3001 caaaaaatta gaaagtcaag ctgacaaagc cttgacgata ctgtctaaat tagggataat
     3061 agaaattgct tttataacac taaccaagga gaatatatga aagaacgtat aaaggacttt
     3121 atctctgtca cactcggatc aattgtcatg gccattggat ttaacagttt ttttctagag
     3181 aataatatcg tatctggtgg tgtcggaggt ttagcaatcg cactcaatgc tcttctgggg
     3241 tggagtccgt ctgattttgt cctctactgt aacatccctt tattaatcat ctgttggttt
     3301 ttcttaggaa aatctgtatt tatcaaaact gtctatggag ctattatcta ccctctttgc
     3361 atcaagttga cagcaggttt acccaacttg acagaaaatc ctctcctagc tgctattttc
     3421 ggtggaatta tcctaggttt tggacttggg cttgtcttcc ttggaaattc ttctactggt
     3481 ggaacaggta ttcttattca atttattcac aagtacaccc ctttatcatt aggtctgaca
     3541 atggctatta ttgatggtat tatcgttggt cttggatttg ttgccttcga tactgatact
     3601 gttatgtact ctattattgc cctaatgacc attacttata ttgtcaaccg catgatgtct
     3661 ggtacccaat cttctcgaaa tgtcatgatt atttctcaaa aatcagaaga aattaaagac
     3721 tatattacta aagttgctga tcgcggtgtg acagaacttc caattattgg tggctttaca
     3781 ggggttgaca agcgcatgtt gatgactacc atctctattc cagaaatgca gaaactggag
     3841 acagcggtat tagaaattga tgaaactgcc tttatggttg ttatgcctgc gagtcaggtg
     3901 cgtggacgtg gcttcagtct tcaaaaagac cataaacatt atgatgaaga tattttgatt
     3961 ccaatgtaat tcattgaaaa ttcaagttct ttctagtaca ataaaactaa tagacagaaa
     4021 tagaggagaa actatgctta cagtatcaga cgtgtcgctt cgctttagcg accgcaaatt
     4081 atttgacgat gtcaatatca aatttacagc aggaaatacc tacggtttga ttggtgccaa
     4141 tggtgctggc aaatctacat ttttgaaaat tcttgctggt gacatcgagc catcaacagg
     4201 tcatatttca cttggaccag acgaacgcct ttctgtacta cgtcagaacc atttcgacta
     4261 tgaagatgag cgcgtgattg acgttgttat catgggaaat gagcaacttt acagcatcat
     4321 gaaagaaaaa gatgctattt acatgaagga agatttttct gatgaagatg gtgttcgtgc
     4381 tgctgaatta gaaggtgagt ttgctgaact tggtggctgg gaagctgaaa gtgaagcttc
     4441 tcaattgctc caaaacctca acatttcaga agacctccac taccaaaaca tgagcgaatt
     4501 gaccaacggt gagaaggtca aggtcctctt ggccaaggcc ctctttggta agcctgatgt
     4561 tcttcttttg gacgagccaa ccaacggtct ggacatccaa tctatcaact ggctcgaaga
     4621 tttcttgatt gactttgaaa atacggttat cgtcgtgtca cacgaccgcc acttcttaaa
     4681 caaagtatgt acccatatgg ccgaccttga cttcggtaaa atcaagattt tcgttggtaa
     4741 ctatgacttc tggaaacaat ccagtgaact agcagctaaa ctacaagctg accgtaatgc
     4801 caaagcagaa gaaaaaatca aagaactaca agaattcgtt gcccgtttct ctgctaatgc
     4861 ctctaagtct aagcaagcta cttcacggaa aaagatgctg gacaaaatcg agttggagga
     4921 aattattcct tctagccgta agtatccatt tatcaacttc aaatcagaac gtgaaattgg
     4981 taatgacctc ctaacagttg aaaacttgaa agttgtcatc gatggtgaaa cgattcttga
     5041 caatatcagc tttatcctgc gtccaggtga caagactgct cttattggtc aaaacgacat
     5101 ccaaacaact gctctcattc gtgctcttat gggcgatatt gaatatgaag gtactatcaa
     5161 gtggggtgtc actactagtc aatcctactt accaaaagac aatactcgtg actttgatac
     5221 aaacgaatct atccttgatt ggctccgtca atttgccagc aaggaagaag atgacaatac
     5281 cttcttgcgc ggtttcttgg gacgtatgct cttctcgggt gatgaggtta acaaacctgt
     5341 aaacgtcttg tcagggggcg aaaaagtgcg cgtgatgttg tcaaaactca tgctcctcaa
     5401 gtcaaatgtc ttggtcttag atgatccaac caaccacttg gacttggaat caatttccag
     5461 tctgaatgat ggtttgaaag cttttaaaga gtccatcatc tttgccagcc acgaccacga
     5521 atttattcaa actttagcaa accatatcat cgtcatttct aaaaacggtg ttatcgaccg
     5581 aatcgacgaa acttatgatg aattcttgga aaatgctgaa gtacaagcaa aagtacaaga
     5641 actttggaaa gcataataaa aagaggctgg gacttttgcc cagcctctct tctcagagtt
     5701 cgtgtcaaca tctcagcgca gtggttgatt gccagatttg ttcgtgtttc acactccaaa
     5761 tctggccatt acgactgttg cgaacaaagt tcgccttatc tccaacctca aactgtctcc
     5821 cagacagttt gagctgtgcg ggggtgggag tgaaacagtc tgggaataga ctgtttcagc
     5881 tcaacaacta gaaataaaga cttgttgacg aactcttttt tgaccggtca agttctttcc
     5941 cacttccttt tcttttaaat ctatgaaaaa aatatttaca aaaacatcca tttattatct
     6001 cttatctttc cttattccgc tgactattat ttctatcgtc ttagcatttc aaggaatctg
     6061 gtggggaagt gatacaacaa tattggccag tgatggtttc catcagtatg ttatttttaa
     6121 tcaaacatta cgaaatactt tacacggaga cggctcttta ttttacacat tttcaagtgg
     6181 tttaggactc aatttttatg ctctatcctc ctattaccta ggttcattcc tatctccaat
     6241 tgtctttttc tttgatttac aatccatgcc tgacgctatc taccttgtca ctattgtcaa
     6301 atttggattg actgggttgt caacttattt cagtctaaag ggtatccata aaaatttgaa
     6361 agaagaatgg gcactattac tcgctactag tttttctctg atgagtttta gtacaagtca
     6421 attagaaata aacaactggc tagacgtttt tatacttctc ccactcgttc ttcttggatt
     6481 gcatcgatta ttaaaaaaac agggtccgat tctctactac ataacattaa catgcttgtt
     6541 tatccaaaac tattacttcg gttatatggt ggctattttc ctaacattat ggacattggt
     6601 tcaattatca tggatagata gtcagagaat caaaagattt atcaagttta caatagtgtc
     6661 aattttatct gctctaagca gcatgttcat gttgttacca acctacttgg atttaaaaac
     6721 tcatggtgag acatttacaa aaattgtcaa cctaaaaacc gaagactctt ggtatctgga
     6781 cttctttgca aaaaacttag ttggtagctt tgatacaaca aaatttggtt ctattcccat
     6841 gatctctgtt ggtctcgttc cattaatact cgctttacta ttctttacat taaaagaaat
     6901 aaaaccaact atcaaactct cctatgctct attctttaca ttcattattt caagtttcta
     6961 cctacaacca ctcaatcttt tttggcaagg tatgcatgcg ccaaacatgt tcctctatcg
     7021 ttatgcatgg gctctatcaa taaccgttat ctatttagca gcagagacat tggtacgact
     7081 acgtcaagta agtattaaaa attttactct gattgtttca tttttactca tttgctttac
     7141 ttctaccttt atttttagag atcattatga gtttctaacg gatgtaaatt tcctactcac
     7201 actagaattt ctaatagcct acttcattct ttttgtagca atgattcgct ataaatcatc
     7261 cttaaaatgg attaatattg ttttattgtt cttcactttc ttagaacttg gattacatag
     7321 tcattatcaa gttcagggga tttctgatga atggcatttt cctagccgat caaactatga
     7381 agaaaaattg acagatattg acagtattgt aaagtctaca aagactacaa ctgattcatt
     7441 ttatcgtata gaacgtctat taccacaaac tggcaatgat agcatgaaat tcaattacaa
     7501 cggtatttct caattctcct ccattcgaaa tcgtgcttcc agctcagcac tagataagct
     7561 cggtttccgt tcagacggta ccaatttgaa tcttcgttat cagaataata caattattgc
     7621 tgatagccta ttcggagtca aatataattt agctactacg gacccaaata agtttggttt
     7681 cacactgaat cagagtcagt ctactattaa tctttacgaa aatagtttta atctagggtt
     7741 agccctattg actgagggga tttacaaaga tgtcaatttt acaaatctga ctctcgataa
     7801 tcaaacaaat ttcctcaatc aactaacagg gctatctcaa aaatactacc acactttatc
     7861 agatgttatt tcacaaaata cagttgaact gagcaatcga atgactgtca acaaagttga
     7921 caatgaggat gctgcaaaag caactttctt agtaaatata cctgcaaata gccaagtata
     7981 cttaaatttg ccaaatttaa cattctcaaa tgaaaatcaa aaaaaggttg tcattactgt
     8041 caataatcag tcaagtgagt ttacattaga taatgctttc tctttcttca atgtggggag
     8101 ctttactaca gatgtacaag ttcaagtcga tgtatatttt cctgaaaata atcaagtttc
     8161 ttttgataaa ccacaatttt atcgattgga cttattagct ttccaacagg ctatttccat
     8221 tctccaagaa aaacaagtag taacaaaaac tgatggtaat aaagtaaccg ttgattttgt
     8281 aaccgataaa gaagcctcac tcctccttac tttaccctat gataaaggat ggaatgcaac
     8341 cattgatggt aaacctatca aaatccaaaa agtgcaagaa ggttttatga aagtggatgt
     8401 aagtccaggt caaactaaac tggttttaac ctttgtacca aatggtttct atctaggttt
     8461 actgatttct tttggtgcag tttttgtatt tttctcctat caattcattg gatactatta
     8521 ttctaagaac cgagaatact aaactttctc ggttttttct tgtaaaaagc aaaaaacctt
     8581 gatttctcaa ggtttcctat gctttatcta ctccgccaac agggctcgaa cctgtgacat
     8641 catgattaac agtcatgcgc tctaccaact gagctatggc gga
//
LOCUS       DAZQCJ010000084         8632 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000084 DAZQCJ010000000
VERSION     DAZQCJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.085"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            76..1245
                     /locus_tag="ACHU66_001676"
     CDS             76..1245
                     /locus_tag="ACHU66_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR3389943.1"
                     /translation="MKTRKFAVALATFASAALLAACGNVSTTNTSAAGSSVGDTFKIG
                     YNLELSGAVSSYGQTEEKGANLAVKEINAAGGIDGKKIEVITKDNKSETAEAATVATS
                     LASEGANVVIGPATSGASAASIPALTSAGVPMITPSGTQTNLVVNDKGEVQEYFFRTT
                     FTDGYQGQIMAKYATENLSAKKVVLYFDNSSDYGKGVAEAFKKAYTGEIVSEITFASG
                     DKDFQAALTKLKDKEFDAIIMPGYYNETGTIVKQARGLGIDKPILGSDGFDSPQFTEL
                     ATASAASNVYYLSGFVTSASEKAKAFYDAYVKEYNEEPSMFSALAYDSVYMAAEAAKG
                     AADSAAVKANLAALKDFKGVTGTMSVDKEHNVIKSVYVVSLTNGEESSVDTISID"
     gene            1451..2338
                     /locus_tag="ACHU66_001677"
     CDS             1451..2338
                     /locus_tag="ACHU66_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HFR3389944.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDLYMMGA
                     FMGYFLLNNLNFIADGGTRFFVALILAMVGTAILGVVIEFLAYRPLRNSTRIAALITA
                     IGVSFFLEYTMIKFFTADVKAFPQAIEAVTFNLGPVSVTNIQLIILGVSLALMVALQL
                     IVKRTKMGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLLGLYYNQIE
                     PLMGMTPGLKAFVAAVLGGIGIIPGAALGGFVIGIIETFTYVIEFDTFRDAIVYTVLI
                     IILLVRPSGILGKNVKEKV"
     gene            2341..3288
                     /locus_tag="ACHU66_001678"
     CDS             2341..3288
                     /locus_tag="ACHU66_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HFR3389945.1"
                     /translation="MKQNLKVNAIWLAILLAGFGLIQGLVSAGILNFYYIQIVQQIGI
                     QIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAVGILGKMMPTYGGFAIALIVGMLISG
                     AVALLVGIPTLRLKGDYLAIATLGVAEIIRILIINGGSVTNGAAGIMSIPLFTSWPLV
                     YIFVIITTLVTVNFLRSPMGRATISVREDEIAAESVGVNPTFVKVSAFVFGAMTAAIA
                     GGLHAGYVGTIVPKDFAFMTSVNVLIIIVLGGLGSITGTFVAAIVLGILNVFLKSYSD
                     ISMIIYSLALILLMIFRPGGLLGTKEFSVAALLKKKEVK"
     gene            3288..4052
                     /locus_tag="ACHU66_001679"
     CDS             3288..4052
                     /locus_tag="ACHU66_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3389946.1"
                     /translation="MALLEVKDLTKNFGGLTAVGDVTMELHEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTISLAGTILNGKAPSKIASLGLGRTFQNIRLFKNMTVLENVLIG
                     LGNHGKSEVFASFFRLPAFYKNEEALKTKAIELLKIFDLDGDADTLAKNLPYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTQLIRKIKEEFGITIILIEHDMSLVMEV
                     TERIYVLEYGRLIAHGTPEEIRNNKRVIEAYLGGEA"
     gene            4052..4762
                     /locus_tag="ACHU66_001680"
     CDS             4052..4762
                     /locus_tag="ACHU66_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3389947.1"
                     /translation="MAMLEVKNLSVNYGVIEAVKDVSFEVNEGEVVTLIGANGAGKTS
                     ILRTISGLVRPSAGTISFLGNEIQKVPARKIVADGLSQVPEGRHVFAGLTVMENLEMG
                     AFLRNNREENQANLKKIFARFPRLEERKNQDAATLSGGEQQMLAMGRALMSQPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAIADRGYVLETGKVVLSG
                     TGKELLASEEVKKAYLGG"
     gene            5133..5888
                     /locus_tag="ACHU66_001681"
     CDS             5133..5888
                     /locus_tag="ACHU66_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR3389948.1"
                     /translation="MIKVEEILSTLSPNQKVNYDKVFQKMASTWQEAGLRPKILMHVC
                     CAPCSTYTLEYLTQYADVTIYFANSNIHPKAEYQRRAAVTAQFVKEFNQKTGQSVGYL
                     EADYSPQEFFKKTQHLANEPEGGDRCKVCYDYRLDKTAEKAVELGFDYFGSALTISPH
                     KNSQTINAAGIEVQKLYGTQYLPSDFKKNGGYQRSVQMCEEYDIYRQCYCGCVYGANA
                     QGVNLVQVKKEALEFLKTHRDYSHIPFKVLDKN"
     gene            5996..6586
                     /locus_tag="ACHU66_001682"
     CDS             5996..6586
                     /locus_tag="ACHU66_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3389949.1"
                     /translation="MKLRKVSFILVTFVIGLLSLSSVKAETSMFVPVGQQNVPGVEVA
                     PFRTGSDSIHVHVGSFGYVATYINGERLKVEPVKHELKCPSYTTENCQTKEWYTSGER
                     ADGSGDYVVKLNKKLEEGDVVTLKFADDGNLYFGQLVYKSEKKRVEQTQKEQEDEYAD
                     ALFKRSIEEEENKTWRDRIKDTFQDAWWNFKGWWNS"
     gene            6736..7767
                     /gene="holA"
                     /locus_tag="ACHU66_001683"
     CDS             6736..7767
                     /gene="holA"
                     /locus_tag="ACHU66_001683"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR3389950.1"
                     /translation="MTVIEQIEKLKKEKLELLTVLCGEDIGQYQIAKDLLLRKLDFDA
                     ADLGFAYFDMSEADYHQVDLDLVSLPFFSDEKVVILDYFADLTTDKKRHLTDDELKQF
                     EVYLENPVETTCLVILAPGKLDSKRRLVKLLKRDGLVLEANPLKEMDLKNHFQKEIMR
                     LGLQMGGEVFQYLLEKSNFDFAEISKNLVFLQSYKGKETIEISDIDAAIPKTLQDNIF
                     DLTQLILQSKVDEARNLVRDLRLQGEEEVKLIAIMLTQFRTYLQVQILLEQGRGEQQI
                     VAELSTIMGRKVNPYQVKYALRDSRHLSIGFLKKVVKLLIDTDFQIKTGQFDKDYLFD
                     LLLLKIATA"
     gene            7880..8485
                     /gene="sodA"
                     /locus_tag="ACHU66_001684"
     CDS             7880..8485
                     /gene="sodA"
                     /locus_tag="ACHU66_001684"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase SodA"
                     /protein_id="HFR3389951.1"
                     /translation="MAIILPDLPYAYDALEPHIDAETMTLHHDKHHATYVANANAALE
                     KHPEIGEDLVALLSDVEQIPADIRQALINNGGGHLNHALFWELLSPEKTEISAELAAD
                     IDATFGSFDAFKEAFTAAATTRFGSGWAFLVVNKEGKLEVISTANQDTPIMQGLKPIL
                     ALDVWEHAYYLNYRNVRPNYIKAFFEVINWDKVNELYKAAK"
BASE COUNT         2533 a         1506 c         1945 g         2648 t
ORIGIN      
        1 tttttgttga caattttcag ataattcagt atattagata catcgtaaaa tttaagaaag
       61 atttaaggag ttttcatgaa aacaagaaaa tttgcagtag cgcttgctac atttgcctct
      121 gcagctctac tcgctgcatg tggtaatgtt tcgacaacca atacttcagc cgcaggttct
      181 tctgtaggtg atacgtttaa aattggctac aacttggagt tgtcaggtgc tgtttcatct
      241 tatggtcaaa cagaggaaaa aggtgccaac cttgccgtga aagaaatcaa tgctgcgggt
      301 ggtattgacg gcaaaaaaat cgaagtgatc acaaaagaca acaagtctga aacagcagag
      361 gcagctactg ttgcaacaag cttggcaagt gaaggggcaa acgttgtgat tggtcctgct
      421 acatcaggag cttcagcagc ctctattcca gcattgacat ctgctggtgt tcctatgatt
      481 actccttcag gaactcaaac aaacttggtt gtgaacgata aaggtgaggt ccaagaatac
      541 ttcttccgta caacctttac agatgggtat caaggtcaaa ttatggctaa atacgctact
      601 gagaacctgt cagctaagaa agttgttctt tactttgaca actcttctga ttatggtaag
      661 ggtgtagcag aggcgttcaa gaaagcatac acaggagaaa tcgtttcaga aatcacattt
      721 gcttctggcg ataaagattt ccaagctgct ttgacaaaat tgaaagataa ggaatttgat
      781 gcaatcatca tgcctggtta ctacaatgaa acaggtacaa ttgtgaagca agcacgcggt
      841 ttgggtattg acaagccaat tcttggttca gatggttttg actcaccaca atttactgaa
      901 ttggcaactg catcagcagc atcaaatgtt tactaccttt ctggatttgt aacatcagca
      961 agcgaaaaag caaaagcatt ctatgatgcc tatgtgaaag aatacaatga agagccatca
     1021 atgttctcag ctttagctta cgactcggtt tacatggcag cagaagcagc aaaaggtgca
     1081 gctgattcag cagcagtgaa ggctaatctt gcagctctga aagactttaa aggtgtgact
     1141 ggtacaatgt ctgttgacaa agagcacaat gtcattaaat cagtttatgt tgttagttta
     1201 acaaacggag aagaatcatc tgtagatact atctctattg actaatagtg gatgaaaaga
     1261 gggaattccc tctttttcac ttgtcagaat gtatagaata gtatacatgc ggatataaaa
     1321 tttgggttgg ttaaattttc tgtaatttta atgattaaac tctctgtcta acttgcttat
     1381 tttcagaaaa ttttgtataa tgtaacaatg ctattgagct cgaaaaatat ttagaaagat
     1441 tggtgaacct atgcttcaac aacttgtcaa tggtttgatt cttggttctg tttatgccct
     1501 tttggccctt ggttatacca tggtgtacgg aattatcaaa ctcattaact tcgcccatgg
     1561 tgatttgtat atgatgggtg cttttatggg gtacttccta ttaaataatt taaattttat
     1621 tgcagatggt ggaacgcgtt tctttgttgc tctcattcta gcgatggtag ggacggctat
     1681 tctcggtgta gttatagagt ttttagccta tcgtcctttg cgtaattcaa cgcgtatagc
     1741 agctttgatt acggctatcg gtgtatcctt cttcttagaa tacactatga ttaaattctt
     1801 tacagcagac gtaaaagcct ttccacaagc aatcgaagct gtgactttta accttggtcc
     1861 agtttctgtt acgaatatcc agttgattat tcttggtgta tcgcttgcgt tgatggttgc
     1921 acttcaatta attgtcaaac gtacaaaaat ggggaaagcc atgcgtgcgg tctctgtaga
     1981 tagcgatgca gctcagctta tggggatcaa tgtaaaccgt acaatcagct ttacttttgc
     2041 tcttgggtcg gctcttgcgg gtgctgcggg tgttctccta ggtctctact acaaccagat
     2101 tgagcctttg atggggatga ccccaggttt gaaagccttt gtagcggcag tattgggcgg
     2161 tatcggtatt attccaggtg ctgcgcttgg tggcttcgtt atcggtatca tcgaaacatt
     2221 cacctatgtg attgaattcg atactttccg cgatgcgatc gtatatactg ttttgatcat
     2281 catcctcttg gtgcgtccaa gtggtattct tggtaaaaat gtgaaagaga aggtgtaacc
     2341 atgaagcaaa atttaaaagt taatgcgatt tggttggcta tcttattggc tggttttgga
     2401 cttattcaag gtcttgtatc agcagggatt ctgaattttt actacatcca gattgttcag
     2461 caaatcggta ttcaaattat cttggcagtt ggtttaaact tgattgttgg tttctcaggt
     2521 caattttcac ttggtcatgc tggttttatg gctattggtg cttatgcagt aggtatcctt
     2581 gggaaaatga tgccaactta tggtggattt gctatcgcct tgattgttgg gatgttaatc
     2641 tcaggtgcag tagctctttt agtcggtatt ccaacccttc gtttgaaagg tgactacctt
     2701 gcaattgcga ctctcggtgt tgcagagatt attcgtattt taattatcaa tggtggtagt
     2761 gtaacgaatg gtgcagcagg tattatgtct attccactct ttacaagctg gcctttagtt
     2821 tacatttttg taatcatcac taccttggtg acagttaact ttttgcgcag tccgatggga
     2881 cgtgctacaa tttctgttcg tgaggatgaa attgcggcag aatctgtagg tgtcaatcca
     2941 acatttgtta aagtttcagc ctttgtattt ggtgctatga cggcagcaat tgcaggtggt
     3001 ctccatgcag gatatgttgg gacaattgtt ccgaaagact ttgcctttat gacctccgtt
     3061 aacgttttga ttatcattgt attgggtggt ttgggctcta tcacaggtac ctttgtagca
     3121 gccattgtac ttggtatttt gaacgtattc ctcaagagtt actctgatat ttcaatgatt
     3181 atctattcat tagctcttat tctcttaatg attttccgtc ctggaggtct tttgggaact
     3241 aaggaattca gtgtagcggc tctactcaag aagaaggagg ttaagtaatg gcattacttg
     3301 aagtaaaaga tttaaccaag aacttcggtg gcctgactgc cgttggtgac gtgaccatgg
     3361 aacttcatga aggggaattg gttggtctta tcggtccaaa cggtgctggt aaaacgactc
     3421 ttttcaacct cttaacaggt gtttatgagc caagcgaggg gacaatttct cttgcaggga
     3481 ctattttgaa cggtaaagca ccatcaaaaa ttgcttcgct tggtcttggt cgtactttcc
     3541 agaacattcg tttgttcaaa aatatgaccg ttttggaaaa tgttttgatt ggtcttggca
     3601 accatggtaa atcagaagta tttgcaagtt tcttccgtct tccagcattc tataagaatg
     3661 aagaagcgtt gaaaacaaaa gcgattgaac ttttgaaaat cttcgatttg gatggagatg
     3721 cagatacatt agctaagaac cttccttacg gtcaacaacg tcgtttggaa atcgttcgtg
     3781 cccttgcgac tgaaccaaaa attcttttct tggatgagcc agcggctggt atgaacccac
     3841 aagaaacggc agaattgacc caacttatcc gtaaaatcaa agaagaattt ggcattacta
     3901 tcatcttgat tgaacacgat atgagcttgg ttatggaagt aacggagcgg atatacgtat
     3961 tggagtacgg tcgtttgatt gcccatggta caccagaaga aattcgtaat aacaagcgtg
     4021 taattgaagc ctaccttgga ggtgaagctt aatggcaatg ttagaagtaa aaaatctttc
     4081 cgttaactac ggtgttatcg aagctgttaa agatgttagc tttgaggtta atgaaggtga
     4141 agttgtaaca cttatcggcg ctaatggtgc tggtaaaacc tctatccttc gtactatttc
     4201 aggacttgtt cgtccatcag ctggaacgat ttctttcctt ggaaatgaga ttcaaaaagt
     4261 tcctgctcgt aaaatcgttg cggacggttt gtcgcaagtt ccagaaggtc gacatgtctt
     4321 tgcaggcttg actgttatgg aaaacttgga aatgggtgcc ttccttcgca acaatcgtga
     4381 agaaaaccaa gcaaacttga aaaaaatctt tgctcgtttt ccacgtttgg aagaacgtaa
     4441 aaaccaggat gcagctaccc tttcaggtgg ggagcaacag atgttggcca tgggacgtgc
     4501 cctaatgagc caaccaaagc tcttgctcct tgatgaacca tcaatgggct tggctccgat
     4561 ctttatccaa gaaatttttg atatcatcca agatattcaa aaacaaggaa caactgtcct
     4621 cttgattgag caaaatgcca acaaagccct tgctatcgca gaccgtggct acgtcctaga
     4681 aacaggaaaa gtcgtcctct caggaacagg aaaagaactc ctcgcctcag aagaagtcaa
     4741 aaaagcatat ctgggtggtt aaaacgtggg ttaccccacg ttttaagttg gagatgagaa
     4801 acgccggcag gcgtttttct ttatatcaag gtctggggga cctttttagg ttggaaatga
     4861 gaaccgctgg taagcggttt cctttataag tgggttaccc cacgttttaa gttggagata
     4921 ggaaaagaaa aagtctttag aggctttttc tttgtccccc aaccctgacg ccttatttta
     4981 ggttggaaat aggaaacgcc gggaggcgtt tttctttata tcaaggtctg ggggaccttt
     5041 ttaggtggaa atagggagtg ttctctcatg ttattgctat ctatgcagtg gtcttcaaat
     5101 atagtataat gaatgtatat ttggaggttt gtatgattaa agttgaagag attttatcga
     5161 ctctatcacc gaatcaaaag gttaactatg ataaagtgtt tcaaaaaatg gcaagcacgt
     5221 ggcaggaagc tgggcttcga ccgaaaattc tcatgcatgt ctgttgtgcc ccatgctcga
     5281 cctatacatt ggagtacttg actcaatatg cagatgtaac catctatttt gcgaattcca
     5341 atattcatcc aaaggcggaa taccagcggc gggcagccgt gacggcccag tttgtcaaag
     5401 aatttaatca gaaaactgga cagtcggtag gctatctaga agctgactat agccctcagg
     5461 aatttttcaa gaaaactcag catcttgcca atgagccaga gggaggagat cggtgtaagg
     5521 tttgctatga ttatcggttg gataagactg cggagaaggc agttgagctt ggctttgact
     5581 attttggttc ggctttgaca atcagtccac ataagaattc gcagaccatc aatgctgctg
     5641 ggattgaggt gcagaaatta tacggtaccc agtatcttcc gagtgacttt aagaaaaatg
     5701 gaggctatca gcgttcagtt caaatgtgtg aagagtatga catttatcgc cagtgctatt
     5761 gtggctgtgt ctatggggcc aatgcccaag gtgtcaatct cgtacaggta aaaaaagaag
     5821 ccttggaatt tttaaaaacg cacagagatt attcccacat tcctttcaag gtgttggata
     5881 agaattgagt ttttgagttt agtttttact taaggagcgt tttttcttgc tttctctatg
     5941 aaattggtat atactaattc cggttaatat attgtactac taggagaaaa gcaaaatgaa
     6001 attgagaaaa gtatcgttta ttttagttac ctttgtcatc ggtttactat cgttatcaag
     6061 tgtaaaggcg gagacttcga tgtttgtacc agtaggtcaa cagaatgttc cgggggtaga
     6121 agtagctcct tttagaactg gttcagactc tatccatgtc catgttggtt catttggtta
     6181 tgttgctaca tatataaatg gagaacgatt gaaggtcgag cctgtaaagc atgaactcaa
     6241 gtgtccgagt tatactacag agaattgcca aactaaagag tggtatacaa gtggggaacg
     6301 tgcggacggc agtggagatt atgttgtaaa attgaataag aaactcgaag aaggtgatgt
     6361 tgttacatta aaatttgcag acgatgggaa tttgtatttt ggtcaattag tatataaaag
     6421 tgaaaagaag cgagttgaac agactcaaaa ggaacaagag gatgagtacg cagatgcctt
     6481 gttcaaacgt tctattgagg aagaagaaaa taagacttgg agagatcgga taaaggatac
     6541 ttttcaagat gcttggtgga atttcaaagg gtggtggaat agctaacaaa ggaactgcta
     6601 tttggaaaat aggtcatttg gagtgattat caattaattt tttctacctt cctgatataa
     6661 atttcaatga aagagtccag actttatgtc taggctcttt ttgtggtaca atgagaaaag
     6721 aattttgagg tttttatgac agtaattgaa cagattgaaa aattaaaaaa agagaaatta
     6781 gagcttctga ccgttttatg cggtgaagac atcggacaat accaaatagc gaaagactta
     6841 cttctgcgga agcttgattt tgatgcggca gatttaggtt ttgcctattt tgatatgtcg
     6901 gaggctgatt atcatcaggt agacttggac ctagtttctc ttcccttctt ttcagatgaa
     6961 aaagtagtga ttcttgatta ttttgcagat ttaacgacag ataaaaaacg gcatttgaca
     7021 gatgatgaac tcaaacagtt tgaggtctat ttggaaaatc ctgtcgaaac aacatgtttg
     7081 gtcattctag caccgggtaa gttggacagt aagcgtcgtc tggtcaaact cctcaaacga
     7141 gatggactag tattagaagc caatcctttg aaggagatgg atttgaaaaa tcatttccaa
     7201 aaggagataa tgcgtcttgg attgcagatg ggaggagagg tttttcaata tcttttagaa
     7261 aagtccaatt ttgattttgc agaaatcagt aagaacctag tctttcttca atcttataag
     7321 ggaaaagaga cgattgaaat ttcagatatt gatgcggcta tccccaaaac actacaagat
     7381 aatatctttg atttaacaca gttaattcta caatccaagg tagacgaggc tcgcaactta
     7441 gttcgtgact tgcggttgca aggggaggaa gaggttaagt tgattgccat catgttaacc
     7501 caatttcgaa cctatctaca ggtgcaaata ctactagagc aaggccgagg ggaacagcag
     7561 atagtagctg agttatcaac tattatgggg cgtaaggtga atccctacca agtaaagtat
     7621 gctttgcgtg attcacgcca tctgtccatt ggatttttga aaaaagtcgt caagctccta
     7681 atcgatacag attttcaaat taaaacaggc caatttgata aggattattt atttgatttg
     7741 ctattattaa aaatcgcgac tgcatagtaa aaagagtatt cttagatcga gggtcaaccg
     7801 tggtttcaga caaataaatt gaaaaggctc tcacattgga gtagaataga actatcactt
     7861 agtataggag aaaacgaaaa tggcaattat tttaccagac cttccatacg catacgatgc
     7921 cttggaacca catattgatg cagaaacaat gacccttcac cacgataagc accatgcgac
     7981 ttatgttgca aatgctaatg cagcacttga aaaacatcct gaaattggcg aagacttggt
     8041 agctcttttg tcagatgtgg agcaaattcc agctgacatc cgccaagctt tgataaacaa
     8101 cggtggtggt catttgaacc atgctctttt ctgggaattg ctttcacctg aaaaaacaga
     8161 aatttcagca gaattggcag cagatattga tgcaaccttt ggttcatttg atgctttcaa
     8221 agaagccttc actgcagcag caacaactcg tttcggttca ggttgggctt tcttggttgt
     8281 taataaagaa ggtaagttgg aagttatctc aactgctaac caagacacac caatcatgca
     8341 aggtttgaaa ccaatcttgg cattggatgt ttgggaacat gcttattacc tcaactaccg
     8401 caatgtgcgt ccaaactaca tcaaagcctt ctttgaagta atcaactggg acaaggttaa
     8461 cgaactttat aaagcagcta aataagaata gttctcttcc tttgggaaga gattttttag
     8521 ttgccaggat tatttttgag agaattgtgt aagggaagaa ggggaatttt cgtattatag
     8581 ataaaaatga caagatattt tcagaaaaat gatgtaaaag taagaataaa cg
//
LOCUS       DAZQCJ010000085         8579 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000085 DAZQCJ010000000
VERSION     DAZQCJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8579
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.086"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..1141
                     /locus_tag="ACHU66_001685"
     CDS             <1..1141
                     /locus_tag="ACHU66_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HFR3389952.1"
                     /translation="EKFIQQYLDYLKELGADAIAESQKVLQDLNKYTDSDIMKSQIIE
                     LLDLTILPVSEPHSSEEIVEKIKGAIDEIRSALIIDAKKLKENEFYGHYTTIDTLVNF
                     LFKPETEENKNCPTYLRLSNANQLNDPMEGKALLAILGLDAHMEQYYKPTNVFLSSLT
                     VVKDSLPMWKEYAEESRGAFLEYDQDYLETIVKHDVIEFAKVHYITTDEKQNITGDKL
                     VDQKLIYIQDTIKYLEDEGATDEIREIIDYIAKISYLFKVSDYQYESEYRILVNLDDT
                     HFTNKFTDKLNSGIKDEATRRKLDIGIGLLENKNVNYLNSRKYIHIEKTEQGKCNLYA
                     YINVVPLKYSKIMLGPKVENVDYIAPYIHYIQPDIIVETSSIPYR"
     gene            1190..1282
                     /locus_tag="ACHU66_001686"
     CDS             1190..1282
                     /locus_tag="ACHU66_001686"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HFR3389953.1"
                     /translation="MEGQMQEAAEVLDFELAAQIRDMVIELKNM"
     gene            1469..2206
                     /locus_tag="ACHU66_001687"
     CDS             1469..2206
                     /locus_tag="ACHU66_001687"
                     /inference="COORDINATES: protein motif:HMM:NF016529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HFR3389954.1"
                     /translation="MKNIFGEKRQRFGFRKLSIGLVSAAVASLFFASSVAAAPSASAQ
                     SINYSYVTEQELTDAEKELIIRDLPGLAQETDANYYLIYRPATGTTSTPSTSTSQVLP
                     NTGSVETGLLVAGGVSLLLLAVRFGKKGKKELAGVILLTATGASFFGPTSSALTSQIL
                     ARYNHAIEISAGQALPAPAEIDGYVYVGYLKDSKAIEQTTSEGIRTETIVNKTEVIPF
                     EIQTVENPNYLRGQSEWYRKDRMGSVL"
     gene            2170..7503
                     /locus_tag="ACHU66_001688"
     CDS             2170..7503
                     /locus_tag="ACHU66_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HFR3389955.1"
                     /translation="MVQEGQDGERTVTIKQVHSKGQIISEEEISSTVTKTAVSKIVEV
                     DTKQATDDIVTEVPDTAPSHELPTLQITEEVSTYTESIPFETQEVADDTLLEGTRQIV
                     QEGKAGQKTIETKKTYIDGILVKTETVSETINQPAAPQIIHVGTKPVTSVPDSAPSHE
                     VPTISLTEETHTHTEAIAFDVQEIYDANLAEGSREVEQKGQAGVRTIETKNYYADGVL
                     IKSEQVSDVVTKEPVTEVVRVGTKTTDVIGSETIVTTEELPFETTVTETEELYVGEEK
                     FITEGKVGSKEVTTTYQTINGVRQPAPTVTEKVLEEPTTKEVLKGTKPIEGTETETNQ
                     VEIAFETEYVDDPTLLESKTKVVTAGVNGSKTVTTTYQTIKGVRQENSTVTEEITKQP
                     VKQVIARGTKVEKVPQVIITDLVENDDAKSATISYKLTDETANFQRAVALLYDNTGAL
                     VQEQTITDPNGQLTLENLDFYTDYTVKTKIFYTMAEQEQSSEQEAILESIRKFDLVYK
                     KIEIKDIDAVTVYRRKNGSYIGQEFLEELPTSTDELFIKVTSDRFKEVYLPVSSIEET
                     TLNGKAVFKLVSSFDELVQDKDAQYVANREFYIPKMATDANTYTSFKALLDAMKANPS
                     GTFKLGAHLDASEVPVGDVASYVTNFSGTLDGLNDGYAFSISNLKAPLFFNLGGKVQN
                     LDIKNASLNTSSKNPLATIAINANGATITNVAVEASIKGPQNVSGLVHSATNTTIKGV
                     SFKGSIEVTGTNASLTGGILGNGTMASVGNAKVDATITLPGTENQVAGGIVGRTMLVY
                     DVPGSVYNSYATGSIVTNGTEATIGGIAGSNQVTGDLAPHVGNLTNVVSTMNGPTSLV
                     GLVVNPTGKIKDGFTTTSDTLGNVKAITNEEAQAKVEAMKIQATSSDSKPLNLNPYSV
                     NYLTLDKAQADHETAYYNMEKILPFYNKELLVYYGNKIAVDDKLNKVRLLDVVPMKDT
                     TFIADVHREKANINKIMLHYADDTVDYKAVSYLEDFKNNHVVEYTISGTDLIYTPESF
                     LNDRSALVNDLVSSLSSVVLDSDAMKAVINYPTKLNADTQTGTAKDFYFGDSYNQVMA
                     NLESNVRKILVASLNGQGQASEDYIKEKITNNKAAFVLGLTYLNRWYDINFGEMNTKD
                     LTIFEPDFFGNDVASALDMILAIGNGGYDVLRAHNNVTTFASIIGKQNNQTKLFDMLE
                     DYRQLFLPEMTNNEWFKQTTKAYVVEGKSLIPEVAAKQETTDTYSKYNVGAYSKIVND
                     TVSNPTWKYNHMLLPLLTLPQENIFIITNMNTIAIGSYEHYVDDYSTVENRDKVRQMV
                     DLAAERQRDNADFWYKILDETNRDKLFRSVLNNEGYMMFGPDGTKAYRNLTADVDAIQ
                     DFYGPINKWYREHPSIKTAYADGSETYYITYDMLSDYGTALYTHEMVHNQDGDIYLKG
                     YGRRIGQGMEVYAQGLLQNVFNVTEMNLGFNAVYNSDDANRVHVGDPVARFNSEADFN
                     EYYHNQFDVLYLLDYLEGTNILAQSDAAKKAWLRKIENYYVQNNGVDTHAGNSARALT
                     DAEVASLKSFNDLIDQSIIVQRQYVNNPTNTSKKWDRNSYVSVPMFAANFSALSNSNG
                     SPGDIMFRRMAFELIAAKGYTDGFVPYASGQLSDLAMEKGSKIYDTWTKKDVGLITDD
                     HVLEYIYQGRYATWADFKKAMFAERLAKVAAGQLKPFTMQYELGVADSTKEVTITNFE
                     QLQTLMKEAMEADIQANSLNLNNSRVHALKVKVYQALMNSTNDFRTSIFNP"
     gene            7577..8080
                     /locus_tag="ACHU66_001689"
     CDS             7577..8080
                     /locus_tag="ACHU66_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFR3389956.1"
                     /translation="MMIKPLETKSEILGKAYVHWKSWQEAYADLLPQEFLKNTYTLER
                     CQDWAVRYPQNILIAVMDDKVVGFACYGSSSQEDLQGAGELYALYVLADYYDQKIGYQ
                     LMQAALEKLQSYETVTLWVLEGNARAIAFYEKVGFRFDGVKKTVNLGAERTEYRMIFK
                     QKERENG"
     gene            8073..8540
                     /locus_tag="ACHU66_001690"
     CDS             8073..8540
                     /locus_tag="ACHU66_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HFR3389957.1"
                     /translation="MARKEMVTLTNMCLIEDKEGKVVVQIRDPKRYRWSGVAFPGGHI
                     EEGENFHDSVVREVQEETGLTVTDARLVGLKHWPDKEGHRYIVFLYKATEFTGTIRST
                     EEGEVRWVEKSDLPQMDLAYDLLEILKVMDEPDLSEFFYTRKNDDGEWDKNFR"
BASE COUNT         2798 a         1590 c         1868 g         2323 t
ORIGIN      
        1 tgagaagttt attcaacagt accttgacta tctaaaggaa ttgggggcag atgcaattgc
       61 agaatctcag aaagttctac aagacttgaa taagtatacg gattctgaca ttatgaaaag
      121 tcagataata gagttactag atttaactat cttgcctgtt agcgaacctc attcaagcga
      181 ggaaatcgtt gagaaaataa agggagctat tgatgaaatt cgttctgcat tgataattga
      241 tgcaaagaaa ttaaaagaga atgaatttta tggccactat actacaatag acaccttggt
      301 taatttctta tttaaaccag agacggaaga aaataaaaat tgtccaacct atttacgttt
      361 gtcaaatgct aatcaattga atgatccgat ggaaggaaaa gccttactcg ccatccttgg
      421 tttagatgcc catatggaac agtattataa accaaccaat gtgtttcttt catccttgac
      481 agttgttaaa gatagtttac ctatgtggaa agaatatgct gaggaatcta ggggagcatt
      541 tctggaatat gatcaagatt acttggaaac tattgtcaag catgatgtta ttgaatttgc
      601 taaagttcat tatattacta cagatgaaaa gcaaaatatt actggtgata aactagttga
      661 tcaaaaacta atatatattc aagatacaat taagtattta gaagatgagg gagcaaccga
      721 tgaaattcga gaaatcattg attatattgc taagatatcc tatcttttta aggtttctga
      781 ttatcaatat gaatctgaat atcgtatatt ggtaaactta gatgatacgc atttcactaa
      841 caaattcact gataaattga actctggaat aaaagatgaa gctacacgta ggaaactaga
      901 tattggcata ggattgttag aaaataagaa tgtgaattat ctgaacagca gaaaatatat
      961 tcacatagaa aaaacagaac aagggaaatg taatttatat gcgtacatca atgttgtccc
     1021 attaaaatat tcaaagatta tgttggggcc aaaagttgaa aatgtagact atatagctcc
     1081 ttatatccat tacattcagc ctgacattat cgttgaaact tcaagtattc cgtatcgata
     1141 acctcttttg aaaagctacg aagaattgag ttagaccata agtaagaaac tggaaggtca
     1201 gatgcaggaa gcggcagaag tgcttgactt tgaactggca gcccagattc gtgatatggt
     1261 catcgagttg aagaatatgt aataagagtt gctaagttca gtgatttaag tagaatgaaa
     1321 ttacaagagg cggcaggtag aaattgacct gccacctttt ataatactta aaatttttac
     1381 ttggagactt agggacaaaa aatttgtccc tgtccctata aaaggttata atggtttcta
     1441 tactatttag ggaatagggg gagtttttat gaaaaatatt tttggtgaaa aacgacagcg
     1501 gttcggtttt cggaaactgt caattggact tgtctcggca gctgtagcaa gtttgttttt
     1561 tgcgtcatcg gtcgcagcag caccgtcagc tagtgctcag tctatcaatt attcctatgt
     1621 gacagagcaa gaattgacag atgcagaaaa ggagttgatt attcgtgact tgcctggctt
     1681 agctcaggaa acggatgcaa attattacct gatctatcgt ccagctactg gcacaacatc
     1741 tactccatcg acaagcacgt ctcaagtttt gccaaatact ggctctgtag aaaccggact
     1801 attggtggcg ggtggagtgt ctctcctgtt attggctgtt agatttggta aaaaaggtaa
     1861 gaaggagttg gcgggtgtca tccttttgac agctacggga gcaagcttct tcggtccaac
     1921 tagctccgca ttgacgagtc aaattttggc tcgatataac catgcaatcg aaatatctgc
     1981 cggacaagcg ttaccggccc cagctgagat tgatggctac gtttatgtag gatatttgaa
     2041 ggatagcaaa gctattgaac aaactacttc agaaggaatc cgtacagaaa ccattgtcaa
     2101 caagacagag gtcattccat ttgaaattca aaccgtagaa aatcccaatt atctgcgggg
     2161 acagagcgag tggtacagga aggacaggat ggggagcgta ctgtaacgat taaacaagtc
     2221 cattcaaaag gtcaaattat ttcggaagaa gagatttcct cgactgttac aaaaactgct
     2281 gtttcaaaga ttgttgaagt tgatacaaaa caagcaaccg atgatattgt tacagaagta
     2341 ccagatacag cgcctagtca cgagttacct accttacaga taacggaaga agtatctacc
     2401 tacacggagt ctattccttt tgaaacacaa gaagttgctg atgatacctt gctagaaggt
     2461 acaagacaga ttgtgcaaga aggtaaagct ggacaaaaga caattgagac aaagaaaacc
     2521 tacatcgacg gtattttagt aaaaacagaa actgtctctg aaacgattaa ccaacctgct
     2581 gctccacaga ttatccatgt tggaacaaag ccagtcacaa gtgtaccaga ttcggcacct
     2641 agccatgaag ttccaacgat ttctttaacg gaagaaacac atactcatac agaagctatt
     2701 gcctttgatg tgcaagaaat ctatgatgca aacttggcag aaggcagccg tgaagttgaa
     2761 cagaagggac aagctggtgt tcggacaatc gagactaaga attattatgc cgatggtgta
     2821 ctaattaaat ccgaacaagt ttcagatgta gtgacaaaag aaccagtaac agaagttgta
     2881 agagtcggta cgaagacaac agatgttatt ggatctgaaa ccattgtaac aacagaagag
     2941 cttccgtttg aaacgacagt cacagaaaca gaagaacttt atgttgggga agaaaaattc
     3001 attactgaag gtaaggttgg ttctaaggaa gttaccacta cttatcagac aatcaacgga
     3061 gtccgtcaac cagccccaac agtgactgag aaagtccttg aagagcctac aacgaaagag
     3121 gttctcaaag gaacaaaacc gatagagggt acggaaaccg agactaatca agttgaaatt
     3181 gcctttgaaa cagagtatgt tgatgacccg acattgttag aaagtaagac caaagttgta
     3241 acggcgggtg taaatggctc taagacagtt acaacaactt accagactat caagggtgtt
     3301 cgtcaggaaa attctactgt aacagaagaa attaccaaac aacctgttaa gcaagtcatt
     3361 gcgcgtggta caaaggttga aaaagtacca caagtgatta tcacagattt ggtggaaaat
     3421 gacgatgcca agtcagcgac aatcagctac aagttgacag acgaaaccgc taatttccaa
     3481 cgagctgttg ctctcctcta tgataataca ggtgccttgg ttcaagaaca gaccattaca
     3541 gatccaaatg gccagttgac acttgaaaat cttgatttct acacagatta cactgttaag
     3601 actaagattt tctacacaat ggcagagcag gaacaaagtt cggaacaaga agctattctt
     3661 gaaagcatac gcaaatttga tttggtctac aagaagattg aaatcaagga tattgatgcg
     3721 gtgactgttt accgtcgtaa aaatggtagc tacatcggtc aagaattctt ggaagaactg
     3781 ccaacatcca ccgatgaatt gtttatcaag gtgacttcag accgcttcaa ggaagtatac
     3841 ctacctgtgt caagcattga agaaacaacc ttgaatggca aggcagtctt caaactagtt
     3901 tcatcctttg atgaattggt acaggacaag gatgcccaat acgttgccaa ccgtgaattc
     3961 tacattccta agatggcgac tgatgcaaat acctacacat ccttcaaagc tttgctcgac
     4021 gctatgaagg caaatccaag tggtaccttt aaactaggag cgcatttgga cgcaagtgaa
     4081 gttcctgttg gagatgttgc atcgtatgtg accaacttta gcggtacctt ggatggctta
     4141 aatgacggct acgccttttc tatcagcaac ttgaaagcac cattgttctt taaccttggt
     4201 ggtaaagttc aaaacttgga catcaaaaat gcaagcctga atacaagctc taagaaccca
     4261 cttgcgacaa ttgcgattaa tgccaatgga gcaactatta caaacgtagc agtagaagca
     4321 tctatcaaag gaccgcaaaa cgtatctggt ttagttcatt ctgcaacaaa tactactatt
     4381 aaaggtgtat ccttcaaggg tagtatcgaa gtaaccggaa ccaatgcaag tctaactggt
     4441 ggtatcttgg gtaacggtac gatggcaagt gtcggaaatg ccaaggttga tgcgaccata
     4501 accctcccag gtacagagaa tcaagtagct ggtggtatcg tcggtagaac tatgctcgtt
     4561 tatgacgtac caggaagtgt ctataattcg tatgcaacag gttcaatcgt gacaaatggt
     4621 acagaggcaa ccattggtgg aattgctggt tccaaccaag tgactggcga tcttgctcca
     4681 cacgtaggta atttgacaaa tgttgttagc accatgaacg gtcctacaag tctagttggt
     4741 ttagttgtca atccaactgg taagattaaa gacggcttca caacaacttc agatacattg
     4801 ggcaatgtca aggctatcac gaatgaagaa gcacaagcaa aagttgaagc catgaagatt
     4861 caagcgacct cgtcagattc caaacctctc aatcttaacc cttattctgt caattattta
     4921 acactagata aggctcaggc agaccatgaa acagcctact acaacatgga gaaaatctta
     4981 ccattctaca acaaggaact ccttgtttat tatggaaata agattgcggt tgatgataag
     5041 ctaaacaagg ttcgtttact ggatgttgtt ccaatgaaag atactacctt tattgcagat
     5101 gtccataggg aaaaagcaaa catcaataag attatgcttc actatgctga tgacacggta
     5161 gattataagg cagtttcata ccttgaagac ttcaagaata atcatgtggt cgagtacacc
     5221 atttcaggta cggacttgat ttacacgcca gaatcattct tgaatgacag aagtgccctt
     5281 gtcaatgact tggtaagcag cctttcttct gttgtcctcg actcggatgc tatgaaggcg
     5341 gttatcaatt atccaacaaa attaaatgcg gatactcaga ctggcacagc taaagacttc
     5401 tactttggtg acagctataa ccaagtaatg gctaacctag aaagcaacgt tcgtaagatt
     5461 ttggttgcta gtctaaatgg tcaaggtcag gcttctgaag actacatcaa agagaaaatc
     5521 accaataaca aagcagcctt tgtgctcggt ttgacttact tgaaccgttg gtatgacatc
     5581 aacttcggag agatgaatac caaagacttg accatctttg aacctgactt cttcggaaat
     5641 gatgtagcat ctgcccttga tatgattctt gcaattggta atggtggcta tgatgttctt
     5701 cgtgcccaca ataacgtgac cacttttgca tccatcattg gtaaacaaaa taatcagacc
     5761 aagcttttcg atatgctgga agattatcgt cagctcttct tgccagaaat gaccaacaat
     5821 gaatggttca agcaaacaac caaggcttat gttgttgaag gtaaatcctt gattccagaa
     5881 gtagctgcta agcaggaaac gacagataca tatagcaagt acaatgttgg agcttatagc
     5941 aagattgtga atgatactgt atccaatcca acttggaagt acaaccacat gctcttgcca
     6001 ctcttgactt tgccacaaga aaatatcttt atcatcacta acatgaatac catcgcgatt
     6061 ggttcatacg aacactacgt agatgattat tcgacagtgg aaaatcgtga taaggttcgt
     6121 cagatggttg atttggcggc agaacgtcag cgtgataatg cggatttctg gtacaagatt
     6181 ttggatgaaa ccaaccgtga caagctattc agatctgttc tcaataatga aggctacatg
     6241 atgtttggtc cagatggtac caaggcttat cgcaatttaa ctgctgatgt tgatgctatt
     6301 caagacttct acggtccgat caacaagtgg tatcgtgagc atccatctat taagactgct
     6361 tatgcggacg gaagtgaaac ctactatatc acttacgata tgctgtcaga ttatggtaca
     6421 gccctttata cccacgaaat ggttcacaac caagatggtg atatttatct gaaaggctat
     6481 ggtcgccgta ttggtcaagg tatggaagtt tacgcccaag gtcttctaca aaatgtcttc
     6541 aatgttactg agatgaacct tggattcaac gctgtctata atagcgatga tgccaaccgt
     6601 gtccatgtcg gagacccagt tgcacgtttc aatagcgaag ctgatttcaa tgagtattac
     6661 cataaccagt tcgatgtgct gtacttgctg gattacctag aaggtacaaa catcttggct
     6721 caatcggatg cagcgaagaa agcatggttg cgtaagattg aaaactacta tgtccaaaat
     6781 aatggtgttg atacccatgc tggaaactct gcgcgtgcat tgacagatgc cgaagtggca
     6841 agcttgaagt ccttcaatga tttgattgat cagagtatta tcgttcaacg ccaatatgtc
     6901 aacaatccaa ctaacacaag caagaaatgg gatagaaata gctatgtcag cgttccgatg
     6961 tttgcggcta acttctcagc cctttcaaac tcaaacggtt caccaggcga catcatgttc
     7021 cgtcgtatgg cctttgaatt gattgctgcc aaaggctaca cagatggttt tgtgccgtat
     7081 gcttccggac aattatccga cctagccatg gaaaaaggtt ctaagattta cgatacatgg
     7141 actaaaaaag atgttggttt gattacagat gaccatgttc tggaatatat ctaccaaggt
     7201 cgatatgcta catgggctga cttcaagaaa gccatgtttg ctgaacgctt ggcaaaagta
     7261 gcagctggcc agctcaaacc attcaccatg caatacgaat taggtgttgc tgattcaacc
     7321 aaggaagtaa ccatcacaaa ctttgaacag ttgcaaacct tgatgaaaga agctatggag
     7381 gcagacatcc aagcaaatag tttaaacttg aacaacagtc gtgttcatgc actcaaagtg
     7441 aaagtttatc aagcgttgat gaactctaca aatgacttca gaacatcgat ttttaaccca
     7501 taatcataaa caagttggtc ttgtgccaac ttgtttttta atgtatacta gaggaaaaaa
     7561 ttcggaggta cattagatga tgattaaacc actggaaacg aaaagtgaaa tactaggcaa
     7621 ggcctatgtt cattggaaat cgtggcagga ggcctatgct gacttgttac ctcaggaatt
     7681 tttaaagaat acttacacct tagaacgctg tcaagactgg gcagttcggt atcctcagaa
     7741 tatcttgata gctgtgatgg atgataaggt ggttggattt gcctgttacg gctcgtctag
     7801 tcaggaggat ttgcaagggg caggggagct ctacgccctc tatgtcttgg cggactatta
     7861 tgatcagaag ataggctatc agctcatgca ggcagctttg gaaaaattgc agagctacga
     7921 aaccgttact ctctgggtat tggaggggaa tgcgcgtgcc attgccttct atgaaaaagt
     7981 gggcttccga tttgatggtg tcaaaaaaac ggtcaattta ggtgcagaac gtacagaata
     8041 tagaatgatt tttaaacaga aagagagaga aaatggctag aaaagaaatg gtcactttga
     8101 ccaacatgtg tctaatcgaa gataaggaag ggaaggtcgt tgtacagatt cgagatccca
     8161 agcgttatcg ttggtctggt gttgcctttc caggcggaca tattgaagaa ggcgagaatt
     8221 tccatgattc agttgttaga gaagtgcaag aagaaacagg tttaacagtc actgatgctc
     8281 gcttggttgg tcttaaacat tggccggata aggaagggca tcgttacatt gtgtttctct
     8341 ataaggcaac agagtttaca ggaactatcc gatcaactga agaaggtgag gtacgttggg
     8401 tcgagaagtc ggacctacct cagatggatt tggcctatga tttgctagag atactgaaag
     8461 tcatggatga gccagatttg tccgagttct tttatactag aaaaaatgac gatggagagt
     8521 gggacaagaa ttttaggtag aagctagaaa tagcttcttt tttatgttgt ttgaataaa
//
LOCUS       DAZQCJ010000086         8555 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000086 DAZQCJ010000000
VERSION     DAZQCJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.087"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..645)
                     /locus_tag="ACHU66_001691"
     CDS             complement(<1..645)
                     /locus_tag="ACHU66_001691"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFR3389958.1"
                     /translation="MQYKHLLFDLDHTLLDFSRGEEVALTQFLTAMEVEDIQAFKEVY
                     RPLNQGMWKDLEKGNITKKELINTRFSRTFAHFGRQVDGREMALRYQEFIGRQGQIFT
                     GADKLLQELTHRGYQIYAATNGVTYIQENRLLHSPIQSYFKEVFISEQMGTQKPAADF
                     YEKIAEQISGFQFDQALMIGDSLTADIQGGNNAGMDTVWYNPTHMINNSKAVPTY"
     gene            complement(924..1664)
                     /locus_tag="ACHU66_001692"
     CDS             complement(924..1664)
                     /locus_tag="ACHU66_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor 2"
                     /protein_id="HFR3389959.1"
                     /translation="MSKRKIISSLFLSATVLGGLLFTQTPTVKAIELEDAQTMSTSSA
                     QAPIFSPGELIQETNYNDLVQKSVSIEDFSTNSNNEFTWKVKFDPTHWNFKHDKGGYY
                     FIIPEGMKLKKLVDKHTEKDLLTNFPENVNDSKNDSYSQYRHFKKGERTYWDRDFDSQ
                     WGWSAGRASNDKINQWKDENAFSDIYYIDSPRHAGPVTYELEAEVTDQNKTSFPLVAV
                     MKNFYARTSYLSEPTSLAGLDLKVEWPK"
     gene            complement(2106..4385)
                     /gene="pcrA"
                     /locus_tag="ACHU66_001693"
     CDS             complement(2106..4385)
                     /gene="pcrA"
                     /locus_tag="ACHU66_001693"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFR3389960.1"
                     /translation="MNPLLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKMVNPWNILAITFTNKAAREMKERAYALNPATQACLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRSLMKRILKNLNLDPKKWSERSILGTISNAKNDLIDDKAFEAQA
                     GDLYTQIVAKCYTAYQKELRQSEAVDFDDLIMLTLRLFDQNPEVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPESKVVL
                     LEENYRSTKNVLQAANEVIENNRNRRPKKLWTQNVQGDLITYYRARDENDEAIFVAGQ
                     IDQLHREGKAYKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVISY
                     LNIIANPSDNISYERIVNEPKRGVGPGTVDKLRDFATSHSMSLLEASQDIMLSPVKGK
                     AAQAVFDLANLLYNLRNQLDNLTVTQVVEAVLNQTGYIDALAAQNTLEANARIENIQE
                     FLSVTKNFDEKDVEEEETGLDKLTRFLLDLALIADTDDGDTESSEVTLMTLHAAKGLE
                     FPVVFLIGMEENVFPLSRAAEEEDELEEERRLAYVGITRAEQTLYLTNANSRLLFGRT
                     NYNQPSRFIREISSDLLDYQGLARPANTSFKASYVNGRATQFGQGMSLQQALQSRKAS
                     VQPSAKLGGSMPFASGNKSPGSGTNWSVGDIAVHKKWGRGTVLEVSGSGSNQELKINF
                     PEMGLKKVLASLAPISKEE"
     gene            complement(4675..6042)
                     /gene="nox"
                     /locus_tag="ACHU66_001694"
     CDS             complement(4675..6042)
                     /gene="nox"
                     /locus_tag="ACHU66_001694"
                     /EC_number="1.6.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H2O-forming NADH oxidase"
                     /protein_id="HFR3389961.1"
                     /translation="MAKIVVVGANHAGTAAIKTMLTNYGQENEIVVFDQNSNISFLGC
                     GMALWIGEQIGGPEGLFYSNKEELESLGATVYMESPVTNIDYDAKTVTALVNGQEHVE
                     SFEKLLFATGSQPILPPIKGAEIKEGSLEFEATLENLQFVKLYQNSADVIEKLKNKDI
                     NRVAVVGAGYIGVELAEAFQRKGKEVVLIDVVDTCLAGYYDRDLSDIMAKNLEEHGIK
                     LAFGETVKEVAGNGKVEKIITDKNEYDVDMVILAVGFRPNTALAGEGIERFRNGAFLV
                     NKRQETSIPGVYAIGDCATIYDNATGDTSYIALASNAVRTGIVAAHNICGTDLEGIGV
                     QGSNGISIYGLNLVSTGLTLEKATRLGLNAAVTEVTDNQKPEFMEHGNFPVTIKIVYD
                     KDSRRILGAQMAAREDISLGIHLFSLAIQEGVTIEKLALTDLFFLPHFNKPYNYITVA
                     ALGAE"
     gene            complement(6227..6565)
                     /locus_tag="ACHU66_001695"
     CDS             complement(6227..6565)
                     /locus_tag="ACHU66_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HFR3389962.1"
                     /translation="MQVIKRSGEIVEFDPDKIYQAIIKAAQTVYVIDETWRKNLAQVT
                     KKVVLDLEEAHTERPTISMVQSQVEHRLLDAGYISIAEHYISYRLQRDLERNGYGDKI
                     IVHLRFEQVR"
     gene            6732..8522
                     /gene="pepF"
                     /locus_tag="ACHU66_001696"
     CDS             6732..8522
                     /gene="pepF"
                     /locus_tag="ACHU66_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFR3389963.1"
                     /translation="MTNAMPKRQEIDVQLTWDTDILFPTPDNYKENLAAYAKQVTAFE
                     SNYKGKLTDKDTIVSALTEYEKIVILDSRLSHYAFLPLEVDKMNTELASLANEYDLVS
                     AKARPQRDFLYSELGQLDEAFLLELKDEQPQWAAFFDAILRDKPHQLHPLQEELLSNF
                     APTFGQPYNNYGVTKFEDMTFENFEANGQTLDNSYVLFENDYELSHDTEIRRNSAAGF
                     YSTLKKYKNTTAATYLSHIKNEQIEARLRGFDNTIDFLLHSQNVSRDLFDRQIDVIMK
                     ELAPHMRRYVKLVAKAHGLDKITHADLKISLPSEYNQRITPEESKQFLIDCLGILGED
                     YVKMIEQSFDERWIDFAQNEGKATGGFCATLYDGPSYILLSWTGLMNEVLVLAHELGH
                     AGHFQLAKKQSVLSYDPSLYFIEAPSTANEVLTCNTLLKNNQDPGFQAYLISELISRT
                     YFHNMVTHLLEAAFQREVYTRLDNEEYLNGDILCQIKLDVIKEFWGEDFEIGDDAGLI
                     WMRQPHYYIGLYPYTYSAGLTIGTAMAKQLEEHPEEVVEKWLETLSLGASLSAQDLAK
                     HAGVDVSTDQPLKETIAYVGSLVDKLETLI"
BASE COUNT         2407 a         1854 c         1692 g         2602 t
ORIGIN      
        1 ataggttgga actgccttac tattatttat catatgagtc ggattatacc aaacagtatc
       61 catgccagca ttattgccac cttgaatatc tgctgtcaaa ctgtctccaa tcattaaggc
      121 ttggtcaaac tgaaaacctg aaatctgctc tgctattttt tcataaaaat cagccgcagg
      181 cttttgcgtc cccatctgct cagaaataaa tacttcttta aaatacgatt gaatcggtga
      241 gtgaagcaag cgattttcct gtatataggt cacgccgttg gtcgctgcat aaatctggta
      301 gcctcggtgt gtcaattcct gcaaaagctt atctgcacct gtaaaaatct gtccctgccg
      361 cccaataaat tcttggtagc gcaaggccat ctcccttcca tcaacttgcc gaccaaagtg
      421 ggcaaaggtc cgtgaaaagc gggtgtttat tagctctttc tttgtaatgt tccctttttc
      481 caaatccttc cacatgccct ggttgagcgg tcgatagact tctttaaaag cttgaatatc
      541 ttcaacctcc atagctgtca aaaactgggt taaggctact tcttctccac ggctaaaatc
      601 aagaagagta tggtcaagat caaagagtaa gtgtttgtat tgcaagatga taggtccttt
      661 ctaagcagaa actactgaaa cgatgtcagg cagtctatct aaagtatacc acagtttcta
      721 aaaaaaatag ctccgtgtcc taggctctat tatactgttt ttcagatttc agtagctctg
      781 gacgtacggg tttcctcatc atattatgca agcgctagtt tattcaagta aagagaagag
      841 gactcgcaga aaaaggcaat cgtagtagcc ttaactacac acgattgcca ctggttggtt
      901 ttgttctgca aattgtcagt cacttatttc ggccattcaa ctttcaaatc aagtcctgca
      961 agcgaagttg gttcactcaa gtatgatgtt ctagcataga agtttttcat taccgctacc
     1021 agagggaagg aagttttatt ttgatcagtt acttctgcct ccaattcata agttactgga
     1081 cctgcatggc gaggactatc gatataatag atgtcagaaa atgcattttc atctttccac
     1141 tgatttatct tatcattact tgctcgtcca gctgaccatc cccactgact atcgaagtca
     1201 cgatcccaat atgttctttc gcctttttta aagtggcggt actgactgta tgagtcgttc
     1261 ttactgtcat taacgttttc cgggaagtta gttaataagt ccttttccgt atgtttgtca
     1321 acaagtttct tcaatttcat accttcagga ataataaaat aatatcctcc cttatcatgc
     1381 ttaaagttcc aatgtgtagg atcgaacttt actttccatg taaactcatt attgctattt
     1441 gtcgaaaaat cctcaataga tactgacttt tggaccaagt cgttatagtt agtctcttga
     1501 ataagttccc caggagaaaa aatcggtgct tgtgcgctgc tagtactcat ggtttgtgca
     1561 tcctctaact caattgcttt tacggttggt gtctgtgtaa acagcaatcc tcccaatacg
     1621 gtagcactta aaaataaact tgatataatt ttacgtttgc tcataagagt ctccttcttt
     1681 tatttgatct atttttgcgc tagctacaaa aatattggca agctaatcgt cccatttcta
     1741 ggctatccct tccttactaa gatgagagtc tctagcaaat aaaagacttt taactactat
     1801 cagtataaca atacaagtag tattttctct tggagctatc cgtttttttc tatatttgtg
     1861 gtttagcata tggatggtaa gacacactct aaaaacatcc gattcaaaat tcaaaatttg
     1921 cttatctaga tgtcatcaaa gcgtgcaaga ttgtttcgat gctttaagca gtatacaaaa
     1981 aatcacctca aaacagtcag gtcaaaactc attttgaggt gtcatatatc gttctaaaaa
     2041 taatattatg aagacgtcgc aagtcttgta aaatgcgatg tttcctcttt acttttgatt
     2101 catccctact cttccttact aatcggtgct aagcttgcca agactttttt gaggcccatt
     2161 tctgggaaat tgattttgag ttcttggttg ctgcctgaac ctgatacttc aaggactgtc
     2221 ccacgtcccc atttcttgtg gactgcgatg tcaccaactg accagttggt gcctgagcca
     2281 ggcgatttgt tgcctgatgc gaaaggcata ctgcctccga gcttggcaga tggttgtacg
     2341 gatgcttttc gactttgtag ggcttgttgc aagctcatgc cttgtccaaa ctgtgtcgct
     2401 ctgccattga cataagaagc cttaaagcta gtattagctg gacgagcgag gccttgataa
     2461 tcaagcaagt cactggaaat ttctcgaata aagcggcttg gttggttgta gttggtgcga
     2521 ccgaaaagga gtcgagaatt ggcattggtc aggtagagag tctgctcagc acgggtgatg
     2581 cctacgtatg ccaaacgacg ttcttcctcc aattcgtcct cttcttctgc tgcacgacta
     2641 agtgggaaga cattttcctc cataccaatc aagaagacaa ctggaaattc cagacctttg
     2701 gctgcgtgga gggtcataag agtcacttct gaagattctg tatcgccatc atctgtgtca
     2761 gcaatcaggg ccaaatcaag caggaagcgt gttaatttat ctagacctgt ctcttcttcc
     2821 tcaacatcct tttcatcaaa gtttttggta acagaaagga attcctggat gttttcaatg
     2881 cgggcatttg cttccagggt attttgtgct gcaagggcat cgatgtagcc tgtttggttc
     2941 aatactgcct cgactacttg ggtaaccgtc aagttgtcta attgattgcg aagattatag
     3001 aggagattag ctaagtcaaa gactgcctgt gctgccttgc ccttgactgg tgacaacata
     3061 atatcctgag aagcttctag caaggacatg ctgtggctgg tcgcaaaatc acggagtttg
     3121 tcaaccgtac cagggccaac gccacgtttt ggctcattga caatgcgttc ataagagata
     3181 ttatccgatg gatttgcgat gatattcaaa taagaaatca catctcgaat ttccttacgg
     3241 ctgtagaact tggtgccgcc aaccatggta taagggatat tagatttgag cagggcttct
     3301 tcaatggtac gggattgggc attggtacgg tagagaactg caaaatcctt ataggcctta
     3361 ccttctcggt gcaactggtc aatctgccct gcgacaaaaa tcgcctcgtc attctcatct
     3421 ctggcacgat agtaggtaat caaatcccct tggacattct gtgtccagag ttttttggga
     3481 cgtcgattgc ggttgttttc gatgacctca ttggcagcct gcaagacgtt cttggtcgaa
     3541 cgatagtttt cctccaaaag cacaaccttg ctctctggat aatccttttc aaagtccagg
     3601 atattttgca tatccgcacc acgccaacca tagatagact ggtccgcatc accgaccacg
     3661 cagatattct tgaaacgtga tgccaggagc ttgaccaatt ggtactgggc atggttggta
     3721 tcctgatact catcgacatg gatatactgg aatttctgct ggtaataggt caggacctct
     3781 ggattttggt caaaaaggcg gagcgtcaac atgatcaaat catcaaagtc aacggcctct
     3841 gactgtctta gttccttttg gtaagccgtg tagcatttag caacaatctg cgtgtatagg
     3901 tcgcctgcct gggcttcaaa cgccttgtca tcaatcaagt cgtttttggc attagaaatg
     3961 gttcccagga tagaacgttc actccacttt ttcggatcta gattgagatt ttttaaaatc
     4021 cgtttcatga gactgcgttg ctcacctgga tcgacaatcg taaaattgcg attgtaaccg
     4081 atatggtcag catcccgacg caaaatccgc acacacatgg agtggaaggt agctatcaaa
     4141 caggcttgcg tggcaggatt gagggcatag gcccgctcct tcatctcccg tgccgccttg
     4201 ttggtaaagg taatggctag gatattccaa ggattgacca ttttttcatc aatcaaataa
     4261 gcgatacggt gggtcaggac tcgcgtctta cccgaaccag cccctgccat aatcaaaagc
     4321 ggaccttccg tcgtctgtac cgcttctgcc tgtctgtcat tcattccgtt taataatggg
     4381 ttcattttct catactttcc tagaacatgt atttacaagt aattcacttt caaataggaa
     4441 atcgtttata gctaatcaag gtaatttttt gaggaaatac cgactgtatt tttggaaaaa
     4501 caaacgataa atagctgaaa aactaggctt agatgaaagt gctaaactgt aaatacaggt
     4561 tcattataat cataccctcc attataccaa aaattctctc gataggcagg tgcaaaatga
     4621 tggagaacag ccaaaaaagc ccagagtcat ctctgagctt ttgtattcaa ttgattattc
     4681 agcacccaag gcagctactg tgatgtagtt gtaaggtttg ttgaagtgtg gcaagaagaa
     4741 caagtctgtc aaggccaatt tttcgattgt tacaccttct tggattgcca atgagaagag
     4801 gtggataccc aatgagatat cttcacgagc agccatttgc gcaccaagga tacgacgtga
     4861 atctttatcg taaacaatct tgatagttac tgggaagtta ccgtgctcca tgaattctgg
     4921 tttttggttg tctgttactt cagtaacagc agcgttcaaa ccaagacgag tagctttttc
     4981 aagtgtcaaa cctgttgata caaggttaag accgtagata gagataccgt tagaaccttg
     5041 tacaccgata ccttcaaggt cagtgccaca gatgttgtga gctgctacga taccagtacg
     5101 tactgcgttt gaagccaaag cgatgtagct tgtgtcgcca gtagcgttgt cgtagattgt
     5161 tgcacagtca ccgatagcgt aaacgcctgg gattgaagtt tcttggcgtt tgtttacaag
     5221 gaaggcaccg ttacggaagc gctcgattcc ttcacctgca agcgcagtgt ttggacggaa
     5281 accaacagcc aagataacca tgtcaacatc gtattcgttc ttgtcagtga tgattttctc
     5341 aactttacca ttaccagcaa cttctttaac agtttcgccg aaggcaagtt tgataccatg
     5401 ctcttccaag tttttagcca taatatctga caagtcacgg tcatagtacc cagccaaaca
     5461 tgtatccaca acatcaataa gtactacttc tttaccttta cgttggaatg cttccgcaag
     5521 ctcaacaccg atgtaaccag caccaacaac tgcgacgcgg ttgatgtctt tgtttttcaa
     5581 tttttcaata acatctgctg agttttggta caatttaaca aattgcaagt tttcaagagt
     5641 tgcttcaaat tcaagcgaac cttctttgat ttcagctcct ttgattgggg gtaagatagg
     5701 ttgtgaacca gttgcgaaaa gaagtttttc aaatgattca acatgttctt gaccatttac
     5761 aagcgctgta actgttttgg catcatagtc aatgtttgta acaggtgact ccatgtatac
     5821 tgtcgcaccc aagctctcca actcttcttt gtttgagtag aaaagtcctt caggaccgcc
     5881 aatttgctca ccgatccaca aagccatacc acaacccaag aatgaaatat ttgagttttg
     5941 gtcaaataca acgatttcat tttcttgacc ataatttgtc aacatagttt tgattgcggc
     6001 agtaccagca tggttagcac cgacaacaac gattttagcc ataatgtaaa atctcacttt
     6061 cgaaaatata tttttttacg aactatatta tacaatataa atgttaccga tttcaaaaaa
     6121 tatgctcaga atatcacatt tttttcaaaa agtttttgaa atgctatttt ctttaaacaa
     6181 tgtaaagata tataaaagca aaaacctaga tttctctagg tttctcttaa cgtacttgtt
     6241 caaaacgcag atgtacaata atcttatcgc catagccgtt acgttctaaa tcacgttgca
     6301 agcgatagga aatgtaatgc tcagcaatag aaatataccc tgcatctaaa agacgatgtt
     6361 caacttgcga ctgaaccatg ctaatcgttg gtcgctctgt atgagcttct tccaagtcaa
     6421 gcactacttt ctttgtgacc tgagcaagat ttttccgcca agtttcatca atgacataga
     6481 ctgtctgtgc agctttaatg atggcttggt agattttatc tggatcaaat tcaacaatct
     6541 ctccactacg tttaatgact tgcataaaag ctcctttctg gttttcagta aaccctttca
     6601 tacttacatt atctaggttt ttgtgtattt tgtcaagtat tctttttcat aaattttgcc
     6661 ttggcttatt ttcgctgaaa gccctttagt atgatataat ggacctatta aaatgaaata
     6721 aggagaaaat catgacaaat gcaatgccta aacgtcaaga aattgatgtg caattaactt
     6781 gggatacgga cattctattt ccgacccctg ataactacaa agaaaatttg gcagcctacg
     6841 ctaagcaagt gacagctttc gaatcgaatt acaaaggaaa gctaactgat aaggatacca
     6901 tcgtttccgc cttaacagaa tacgaaaaaa tcgttattct tgatagcaga ctttcccact
     6961 atgccttcct tcctttggaa gtcgataaga tgaatactga attggcgagc ctggccaacg
     7021 aatacgacct cgtttctgcc aaggcacgtc ctcaacgaga tttcctctat tcagaacttg
     7081 ggcaattaga tgaagccttt ctacttgaat taaaagatga acaaccacaa tgggctgctt
     7141 tcttcgatgc tattctccgt gacaagccgc atcagcttca tccactacaa gaagaacttt
     7201 tatcaaattt tgctccaaca tttggtcagc cctataacaa ctacggtgtg accaagtttg
     7261 aagatatgac atttgaaaac ttcgaggcaa atggtcaaac gctcgacaat agctatgtgc
     7321 tctttgaaaa tgattatgag ctcagccacg ataccgaaat ccgtcgcaat tcagctgctg
     7381 gtttctattc aaccttgaag aaatacaaga atacaactgc agcaacttat ttgtctcata
     7441 tcaagaatga gcaaatcgaa gctcgcttgc gtggatttga caatacgatt gacttcctct
     7501 tgcatagtca aaatgtctct cgtgatctct tcgaccgtca gattgatgtc atcatgaaag
     7561 aattggctcc tcacatgcgt cgctatgtaa aattggttgc taaggctcat ggattggaca
     7621 agatcacaca tgctgacttg aaaatcagtc taccatctga atacaaccaa cgcatcacac
     7681 ctgaagaatc caaacaattc ttgattgact gcctgggcat tcttggtgaa gattatgtga
     7741 aaatgattga acaatcattt gatgagcgtt ggattgactt tgctcaaaac gaaggtaagg
     7801 ctactggtgg cttctgtgct actctttacg atggaccatc ttacatccta ctttcatgga
     7861 caggcttgat gaacgaggtc ttggtattgg ctcacgaatt gggacatgct ggtcatttcc
     7921 aattggctaa gaaacaaagt gtccttagct acgatccatc cctctacttc atcgaggcgc
     7981 cttctactgc aaatgaagtc ttgacatgta acaccctttt gaaaaacaac caagaccctg
     8041 gcttccaggc ttacttgatt agcgagttga tcagccgtac ttacttccac aacatggtca
     8101 ctcacttgct tgaagcagcc ttccaacgtg aagtctacac tcgtttggac aatgaagaat
     8161 acctcaacgg tgatattctc tgccaaatca agttggatgt catcaaggaa ttctggggtg
     8221 aagactttga aatcggtgac gatgcaggtc ttatctggat gcgtcaacca cactactaca
     8281 ttggtttgta tccatatacc tactcagctg gtttgaccat cggtacggct atggctaagc
     8341 aattggaaga acatcctgaa gaagttgttg aaaaatggtt ggaaaccttg tcactcggtg
     8401 caagtctttc tgcccaagac cttgctaagc acgcaggtgt tgacgtttca actgaccaac
     8461 cactcaaaga aaccattgcc tatgttggtt cattggtgga taaactggaa acactaatct
     8521 aagacacata aaagacttga acaacttgtt caggt
//
LOCUS       DAZQCJ010000087         8476 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000087 DAZQCJ010000000
VERSION     DAZQCJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.088"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..1754)
                     /locus_tag="ACHU66_001697"
     CDS             complement(<1..1754)
                     /locus_tag="ACHU66_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFR3389964.1"
                     /translation="MEVLKQIKQDILNVFPFGDRRLLAKSLLLYLLFSYLGTCLGHHV
                     NNFSDGDFLDRLFQAILEIETMRWIFFPSLDEIITGLTLSVVVGIVLYFRKKNAKKFR
                     NGVEYGSARWGDRKDIQPYEDPVFENNILLTDSEKLTMNSRPSNPKFARNKNILVVGG
                     SGSGKTRFFVKPNLMQMHSSYCVTDPKGTIVLECGKMLEKNGYQIKILNTINFKKSMK
                     YNPFAYLKSEKDILKLVQTIIANTKGEGEKAGEDFWVKAEKLYYTALIGYIYYESPKE
                     EQNFNTLLAMIDASEVREDDENFKNAIDYLFLALERRKPNHFAVKQYKKYKLAAGKTA
                     KSILISCGARLAPFDIQELRELMSDDELELDTLGDRKTALFVIISDTDDTFNFVVSIL
                     YSQLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPRFEKLIATIRSREISAAIIL
                     QAKSQLKAIYKDNADTIIGNCDTELFLGGKEGTTLKELAENLGKETIELYNTSETRSN
                     QKSFGLNYQKLGKELMSRDELKVMDGGKCILEIRGTRPFYSDKYDITKHKHYKLLEDF
                     DKRNKFDIEAYLKNRGQA"
     gene            complement(1766..2260)
                     /locus_tag="ACHU66_001698"
     CDS             complement(1766..2260)
                     /locus_tag="ACHU66_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFR3389965.1"
                     /translation="MINEEVGNRLVTLNLRLAKSSAKMIIAALKLVIKEAESEKLSVK
                     QYISQKSLQGEVPLKDLVGKGTLENLPMKQPELLELKKELNKHGVRFSVLRNKENKQF
                     EVFFQAKDTAVLEHSFKQAVERAERKTKIKQSTLKKIQQYQKSLGQHIIKPKIKQKHQ
                     EQSL"
     gene            complement(2253..4715)
                     /locus_tag="ACHU66_001699"
     CDS             complement(2253..4715)
                     /locus_tag="ACHU66_001699"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HFR3389966.1"
                     /translation="MVSHRNDITGSSKEWGTLAGNQFIIDYTNTSFEVSKAAHSSFSE
                     NYHAIRIPETYPYKLFVNGQPKTLSDLKRDKIGHLTAVIDGREVPLLDYGMVEYQMNR
                     YLGKDIYNIYGNLSYTAYAFLTTRTSHHGYFELTEADNVRDVSGQLVTGFFQTPEYRD
                     MASAYHKSNSGYLKVQVGDQELSTNIVNGRLNGLKTAKQHFYLVSNSEFSMDPQTGKP
                     MKQLRELTEDEVHQLKVTETKVNSEIEFDVNGKTYQTTGRLNFWKNSTINPLKTRLAV
                     MSIGDGNNIHGFDLYASNNNYIEVVEKIAEEPSIPESISKMIEFVTEDGEKVSDGPSI
                     TGNKGEEVTLEITPPDGYTFVSIPPERIVLEDSNPIQIIVNENTGQETLERVEEIDYR
                     VIRQENPDMYVGDEAIVRKGIRGTKTIQDIFETKKGIRTDKLLSSSVISETAPSDEII
                     HYGTKAIEGEEIITRTEITPFNIIEEVDPNLYEGEIVTKVEGQNGEFTYYDYYRTIKG
                     TKELAAFKSEAKEIKPKIDKVVRIGTKQIEGEIIEKSSEKIPFGMIIEEDNSLFEDER
                     VVIREGVPGIVDVVATYKTSKGEKIGEPINVQSFIQREAVDELVKVGTKPLNTTSKVE
                     NLNEIPFTVVKEYDDSLEEGVEKIIKVGKNGQKKIIVTTNYVRGIQDGESIVEEITIE
                     EPQNQVILVGTKTLQKESPSPNNDNTDSQTKIDIPSSDDNLPKVPKIPNNTQSDIEKP
                     IEKETKDSSEINQKGSSQTDVNKSKTTTKLDSNTTNNTNGSKLPETGETLVSPLLIII
                     SSIFCLFTSLGMIIWKGRKSND"
     gene            complement(4828..5787)
                     /locus_tag="ACHU66_001700"
     CDS             complement(4828..5787)
                     /locus_tag="ACHU66_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFR3389967.1"
                     /translation="MNALLFDERPIVANPVLAREIGLNEAIVLQQINFWLEINKQNGK
                     NYHDGKYWTYNSIRAWHESDFSYLSYETVKRTFSKLEKAGYLFTGNYNKDPRDKTKWY
                     SLNYDKIFTLEEEVKQKRNNVASKVLEKADENDVTNALGQNDPMEKFKLIQCNNSERT
                     NGLVQTEPMQSVNMSQPLPNNTSNNTTDNTSFYINQSIEEEDELEKLKKQVQYDLLKE
                     SGCVEKLCEWDEVLPIIADVFYQNEGTISINQMRLPIDFVKERFRQLNMHHIDYICEC
                     LSKVDTNIRNIRAYILTTAFNAPQTIGAYYSNQVKQDFGGSIL"
     gene            complement(6054..7253)
                     /locus_tag="ACHU66_001701"
     CDS             complement(6054..7253)
                     /locus_tag="ACHU66_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR3389968.1"
                     /translation="MATKSSKKYKGVYCDNKGKIFYQIDLGIDPVTGKRVQKKARKNQ
                     YGKTFETMKEAYDELVRIKYEFANKVSLENYNMTFENYMNTIYLRAYKQKVQSVTYKT
                     ALPHHKLFIQYFGSKLLKAITPRDCEAFRLHIIENYSENYAKNLWSRFKACMGYAERL
                     GYISDMPCKALDNPRGKHPETPFWTYAEFQTFIKSFDLNDYEELQRFTAIWLYYMTGV
                     RVSEGLSLCWEDIDFENKFLKVHTTLEKDENGNWYRKDQTKTPAGERLIELDDITIEV
                     LQVWRKNQFANQDTDFIISRFGDPFCKSTICRIIKRKAQQVGVPVITGKGLRHSHASY
                     LINVLKKDILYVARRMGHADKSTTLNTYSHWFNALDKTVSEEITQNIKSAGLDSILCQ
                     NSCQTSN"
     gene            complement(7276..7503)
                     /locus_tag="ACHU66_001702"
     CDS             complement(7276..7503)
                     /locus_tag="ACHU66_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFR3389969.1"
                     /translation="MRTATYKDLMNLGFPEHTSRDIIREAKRIAVKKFEEARKVDHNA
                     VQLSKSPFDNRRLGIAPAEIVEQLIGIPLSK"
     gene            complement(7828..8232)
                     /locus_tag="ACHU66_001703"
     CDS             complement(7828..8232)
                     /locus_tag="ACHU66_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFR3389970.1"
                     /translation="MKYYIHVRGSKIPVTETVYKSYWQLVNHEKYLNRKDRKFGVLPF
                     SSFEVDGLLLANVLPDTTVDVERFIENKLLIEQLNEALLTLSDKEFKIIDALYFREHT
                     IREVAIEHEMSATSLFRMRNTILDNLRKFFEK"
BASE COUNT         2448 a         1634 c         1258 g         3136 t
ORIGIN      
        1 ttggcttggc ctctattctt gagataggcc tcaatatcaa acttgtttct cttatcaaaa
       61 tcctctaaaa gtttgtaatg cttatgctta gtaatatcat acttatcaga ataaaatggc
      121 cgtgtcccac gaatctccag gatacatttg cctccatcca tcacttttaa ctcatctctg
      181 gacattaact cctttccgag tttctggtaa ttaagaccaa atgatttctg attggatctg
      241 gtttcacttg tattgtagag ttcaatagtt tctttaccta agttttcagc caattctttt
      301 agcgtagtac cttcttttcc tccaagaaat aactcggtat cacagtttcc aataattgtg
      361 tcagcattgt ccttatatat ggctttcaat tgagatttcg cttgaaggat gattgccgca
      421 gaaatttcac gagaacgaat tgtagcaatt aacttttcaa atctcggaat taaaccaatg
      481 tttgcaaatt cgtccagcag acagcgaaca tggactggta gacgaccacc ataaacatcg
      541 tctgctttat cgcaaagtaa attaaataac tgagaataca agatggacac aacaaagtta
      601 aaggtgtcat ctgtatcaga aatgatgaca aatagggcag tttttctatc tcccagagta
      661 tccaattcca actcatcatc gctcataagt tctctcaact cttgaatatc aaaaggtgcc
      721 aatctagcac cacatgatat gagaatagat ttggcagttt tacctgctgc tagtttatac
      781 tttttgtatt gcttgaccgc aaagtgattt ggttttcttc tttccaaggc cagaaataaa
      841 taatcaatgg cattcttgaa attttcatca tcttcccgaa cttcactagc atcaatcatg
      901 gccaataagg tattaaagtt ttgttcttct tttggacttt catagtagat ataaccaatc
      961 aaggctgtat agtagagttt ctcggctttg acccaaaaat cttctcccgc tttttctccc
     1021 tctcccttgg tgttggctat aatagtttga actagtttaa gaatatcctt ctcacttttt
     1081 agataagcga aaggattata tttcattgac tttttgaagt taatagtatt cagtattttt
     1141 atctgatagc catttttttc taacattttg ccacattcaa gaacgattgt tccttttgga
     1201 tctgtgacac agtaactaga atgcatttgc attagattcg gcttcacaaa aaatcttgtc
     1261 tttccactac cagaccctcc gacaaccaag atatttttat tccgagcaaa ctttggattc
     1321 gacggtcgag aattcatggt taatttctca ctatctgtca aaagaatatt attctcgaat
     1381 actggatctt catatggctg aatatctttc ctatcacccc atctggcaga cccatattcc
     1441 actccattcc taaacttttt tgcattcttt tttctaaaat aaagtactat tccaacaaca
     1501 actgacaagg ttaagccagt aattatttca tcaaggctag gaaagaatat ccaccgcatc
     1561 gtttcaattt ctaggattgc ttgaaataac ctatcaagga aatccccatc agaaaagtta
     1621 ttgacatgat gtcctagaca ggtaccgaga tacgaaaaaa ggagatagag taataaactc
     1681 tttgcgagta atctcctatc tccgaatggg aaaacattaa gaatatcttg tttgatttgt
     1741 tttaatactt caatcctacc acctcctata aggattgttc ctgatgtttt tgtttgattt
     1801 ttggttttat gatatgttgg ccaagtgatt tttgatattg ttgtattttc ttaagagttg
     1861 actgcttaat ttttgttttt ctctctgcac gttcaactgc ctgcttaaaa gaatgttcta
     1921 agacagccgt atctttagcc tgaaaaaaca cctcaaactg tttattctcc ttattcctca
     1981 ataccgagaa tcgaacacca tgcttattta gttccttttt caattcaaga agttcaggct
     2041 gtttcatagg caaattttct aacgttccct ttccaactaa atcttttaag ggaacttccc
     2101 cttgcaaact tttttgagat atgtattgtt tgactgataa tttttctgat tcagcttctt
     2161 taataactaa ctttaaagct gcaattatca ttttggcgga actttttgct aaccttagat
     2221 ttagtgttac aagacgattg ccaacctcct cgttaatcat ttgatttccg tcccttccaa
     2281 ataatcatcc ccaatgaagt gaataaacaa aaaatactac tgatgataat caacagagga
     2341 cttacaagtg tttctccagt ttctggcaat tttgaaccat ttgtgttatt tgtggtgtta
     2401 ctatccaatt ttgttgtagt tttactttta tttacatctg tttgactaga acctttctga
     2461 ttaatttctg aactatcttt agtttctttc tcgattggtt tttctatatc actctgcgta
     2521 ttatttggta tttttggtac tttaggaaga ttatcatctg atgatggaat atctatctta
     2581 gtctgactgt ctgtattatc attattaggg gaaggggact ctttctgtaa tgtttttgtg
     2641 cctactagga taacttgatt ctgtggctct tcaatcgtaa tttcttctac gatagattcg
     2701 ccatcttgta tcccacgtac ataatttgtt gtcacaataa ttttcttctg tccatttttc
     2761 ccaactttga ttatcttctc aacaccttct tcaaggctat catcatactc tttcacaaca
     2821 gtaaaaggaa tttcattcaa attctcaact ttagaagtgg tatttaaagg ctttgtacca
     2881 accttgacca attcatcaac tgcttcacgt tgaataaatg actgaacatt aattggctct
     2941 ccgatttttt cacccttact agttttatat gttgctacca catctacgat tccagggact
     3001 ccttctctaa taacgacacg ttcgtcctca aataaagaat tatcttcttc aataatcatt
     3061 ccaaatggaa tcttctcgct tgatttttct attatctcac cttcaatttg cttggtccca
     3121 attcttacga ccttatcaat ttttggctta atttccttag cttctgattt gaaagcggcg
     3181 agttcttttg tacctttaat tgtccggtaa taatcgtaat aagtaaactc accgttttga
     3241 ccctcgactt ttgttacaat ttcaccttcg tatagatttg gatctacctc ctcgatgata
     3301 ttaaatggtg taatttcagt tcttgtgatt atttcctcac cttcaatcgc ttttgtgccg
     3361 tagtgaatta tttcatcact tggggcagtc tcagaaataa cacttgagct taaaagctta
     3421 tccgttctga tacctttctt tgtttcaaaa atatcttgaa tagtctttgt gcctcttatt
     3481 ccttttctaa caatagcttc atctccaaca tacatatcag gattttcttg tcgaataaca
     3541 cggtaatcta tctcttctac tctttctaat gtttcctgac ctgtattctc gttaacaatg
     3601 atttgaattg gattgctgtc ctctaataca atcctttcag ggggtattga aacaaatgta
     3661 tatccatcag gtggggtaat ctctaatgtg acctcttctc ctttgttacc agtaatagat
     3721 ggcccatcac ttaccttttc accgtcttca gtaacaaatt caatcatttt agaaatagac
     3781 tcaggaattg agggttcttc cgctatcttt tctacaactt cgatgtaatt gttgtttgag
     3841 gcatacaaat caaaaccatg tatattattt ccatcaccaa ttgacataac tgcaagtcgt
     3901 gtttttaatg gattaatagt cgagtttttc caaaaattta atcgtccagt tgtctgatat
     3961 gttttaccat ttacgtcaaa ttcgatttct gagtttactt ttgtttcagt tactttcaac
     4021 tgatgtactt catcttcagt tagttccctt aattgtttca ttggttttcc agtttgtgga
     4081 tccattgaaa attcactatt tgatactaga taaaaatgtt gtttggcagt tttcagtcca
     4141 ttcaatcgac cgttaacaat attcgtagaa agttcttgat cacccacttg tacttttaag
     4201 tatcctgaat ttgatttatg ataggcagat gccatatcac ggtactcagg tgtttgaaag
     4261 aatccagtta ctaactgacc actaacatcc cttacgttat ctgcttcagt taattcaaag
     4321 tatccatggt gggaagtacg agttgtaaga aatgcgtatg cagtatatga tagattaccg
     4381 taaatattat aaatatcctt acctaggtag cgattcatct gatactctac cataccgtag
     4441 tcaagcaaag gtacttctct gccatctata acagcagtta gatgacctat tttatcacgc
     4501 tttaaatcag ataatgtttt tggctgtcca ttgacaaata atttgtaagg ataagtttct
     4561 ggaattcgaa tagcatgata attctccgaa aaactagaat gtgctgcttt agaaacttca
     4621 aaactcgtat ttgtataatc aataatgaat tgatttccag ctaaagtacc ccattcttta
     4681 ctactcccag ttatatcatt tcgatgagat accacttcat tagcataaac tgtgaataaa
     4741 ttattaggac taagaataat agaatttacg ctaatgaaac caatccaata gttaatttac
     4801 gttggcaaaa tttagttatt tttgaaatca taatatactt cctccaaaat cttgttttac
     4861 ttggtttgaa taataagcac ctattgtttg aggtgcatta aatgctgttg ttagaatata
     4921 ggctctgata ttacgaatgt ttgtatctac ctttgacaaa cactcacaga tatagtcaat
     4981 atggtgcata tttaattgtc taaaacgttc ttttacaaaa tctatcggca atctcatttg
     5041 attgatagag attgtgcctt cattttggta aaacacatca gcaatgatag gtagcacctc
     5101 atcccattca cataatttct caacacaacc tgattctttc aataagtcat actgaacttg
     5161 tttttttaat ttttctagct catcctcctc ttcgatagat tgattgatat aaaatgaagt
     5221 attatctgtt gtattatttg aagtattatt tggtaatggt tggctcatat tgacactttg
     5281 cattggttca gtttgaacca atccattagt tctttctgaa ttattgcatt ggatcaattt
     5341 gaacttttcc attgggtcat tttgacccaa tgcattggta acatcattct catctgcctt
     5401 ttctagaact ttacttgcta cgttatttct cttttgcttt acctcctcct ctaatgtgaa
     5461 aattttgtca tagttcaatg aataccattt tgttttatcc cttggatctt tattgtaatt
     5521 ccccgtaaat aaatagccgg ctttttctag ttttgaaaat gtccgtttta cagtttcata
     5581 acttaggtaa gaaaaatcgg actcatgcca agcccgaata gaattataag tccagtattt
     5641 tccatcatgg tagttcttcc cattctgttt atttatttct agccaaaagt taatttgttg
     5701 aaggacgata gcctcgttca atccaatttc tctggccaag acaggatttg cgactattgg
     5761 tcgctcatca aacaacagtg cgttcataca cacctcctac gtcattcttc aaaaagaaaa
     5821 gcaaccagaa ttttctgatt gctcaataat ctaaaatatt ataaaacccc accacgttta
     5881 atcgtggtgg ggtttgtggt ggggaatcaa atgattccaa accaatttaa atcaccataa
     5941 aggcgttaaa agccttatta cttggtttct cactccaaat atactctaat atattattta
     6001 agagtaacaa ttgcacaacc tgttaaatgt gcagtatcaa ggggtttgag ttcttaattt
     6061 gaagtttggc aggaattttg gcataaaatt gaatccaatc ctgcactttt aatattttga
     6121 gtgatttctt cagaaactgt tttatctaaa gcattaaacc agtgactgta tgtattcaaa
     6181 gttgttgatt tatcggcatg tcccatacgt ctggctacat ataaaatatc ttttttcaag
     6241 acattgatta gataggatgc atggctatgt ctgagacctt ttcctgtaat tactggaact
     6301 cctacctgct gagctttccg tttgataatg cgacaaatgg ttgatttaca aaaaggatct
     6361 ccaaatcttg aaatgataaa atctgtatct tgatttgcaa actgattttt tcgccaaact
     6421 tgaagtactt caatagttat atcatccaat tcaataagtc gttcacctgc tggagtcttt
     6481 gtttggtctt tacgatacca atttccattt tcatctttct ccaaggttgt atgtaccttt
     6541 agaaatttat tttcaaaatc aatatcctcc caacataaag ataaaccttc actcactcgt
     6601 acacctgtca tataatataa ccagatggct gtaaatcgtt gtaattcttc ataatcattt
     6661 aagtcaaatg atttgataaa tgtctgaaac tccgcatagg tccaaaatgg agtttctgga
     6721 tgcttacctc taggattatc taatgcttta caaggcatat ctgaaatata gccaagtctt
     6781 tctgcataac ccatacaagc tttaaacctt gaccataaat ttttcgcata gttttcagaa
     6841 taattttcta tgatatgcaa tctaaaagct tcgcaatctc taggagttat tgcttttaat
     6901 agctttgaac caaaatattg aataaaaagt ttatgatgag gaagagccgt cttatatgtc
     6961 acagactgaa ctttttgctt ataagctctt aaataaattg tgttcatata gttttcaaaa
     7021 gtcatattgt aattttctaa actgactttg ttggcaaatt catattttat acgaactaat
     7081 tcgtcatatg cttctttcat tgtttcaaag gttttaccat attgattttt tctagctttc
     7141 ttttgtaccc ttttgccagt tactgggtcg attccaagat caatctgata aaatatcttt
     7201 cccttattat cacaataaac acctttgtat tttttactac tttttgttgc catttggatt
     7261 ctcactttct aaatgttatt tagatagtgg aatccctatt aattgttcca caatttctgc
     7321 tggtgcgata cctagtcttc gattgtcaaa gggcgattta cttaattgta ccgcattatg
     7381 gtcaactttg cgagcttctt caaacttttt tacagcaatt cttttagctt ctcgaataat
     7441 gtctctagag gtatgttcag gaaatcctaa gttcattaaa tctttatatg tagcagttct
     7501 catacacttt cctccttcaa atcacttagt tttttaacat gctagatgag atttgaatgg
     7561 ttagttcaat catgggaatt tcacccgctg tccttttacg atggcagcct gaacggtcag
     7621 aagtatcatt atcattattt gtgtcatggc agataggtca ccatcctatt tttgaataaa
     7681 tgattttcgc tcatttcttg gcgcattaca ttctattgct caacaaataa ataaagtccc
     7741 acctagaatt attcaattgt caaagggcaa aaagagacta aaaattcctc tcactataaa
     7801 gagtgaattt tttagtctcc tgtttcctta tttttcaaaa aattttcgta ggttgtccaa
     7861 aattgtattt ctcatccgaa atagactagt cgcagacatc tcatgctcta ttgctacttc
     7921 tctgatggta tgctctctaa aatacagagc atcaatgatt ttaaactctt tatcagataa
     7981 ggttaataaa gcctcattta gttgctcaat aaggagcttg ttttctataa aacgttcaac
     8041 atcaacagtt gtatcaggca gtacatttgc tagcagtaac ccatctactt caaatgatga
     8101 aaaaggtaat actccaaatt tcctatcttt tctattcaga tacttctcgt gattgaccaa
     8161 ttgccagtag cttttataaa ccgtttctgt tacggggatt ttactacctc taacgtgaat
     8221 ataatatttc atttcagcct ccgtttatga ttttttctta gtcataaaag gagggctcct
     8281 tatctcaacc atagctattt ctcactttta tcaaatcaag gagttctcct cactataaga
     8341 gtgaaaatat ggtgctcttg ttccaaaaaa aattatatgt accaaaaaac agcaagctct
     8401 tctgaacttg ctgatctata ttttaataag tttctgttta gtaggtaaca acaagatatt
     8461 ttgctatata agcaat
//
LOCUS       DAZQCJ010000088         8106 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000088 DAZQCJ010000000
VERSION     DAZQCJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.089"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..1228
                     /locus_tag="ACHU66_001704"
     CDS             <1..1228
                     /locus_tag="ACHU66_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR3389971.1"
                     /translation="KTFLKCASVCAFASFLVACGNASSSDKVEIEYFSQKPEMQATLQ
                     EIINDFEKENPTIDVKFSNVPDAGTVLKTRMANNEAPDVINIYPQNADFKAYAADGRF
                     LEIGDDAGLSHLKDGAVTPYLVNEKNYTLPLTANAYGIYYNKDKFKELGLEVPTTYAE
                     FVALVDKIKADGSAAPFALSLNDAWSLNGYHQLAWVTVAGGFDGAEDILIRSAKGAIQ
                     DDATTKAVLERLQLLKDNGQKGATGALYADAVASFAAGDALMLPQGTWAATAVNQQEP
                     EFEYGMFTFPGDKDGGDYTIGAADLALSISADTEHPEESKKFLEYLSRPEVMQKYYDV
                     DGSPTSVEGVDTEGKFEETAGVTQYAFTDKHVVWLQSEWESEEEFWNITVEMVKNPNS
                     AELVKKLNAFFDPMKK"
     gene            1363..2235
                     /locus_tag="ACHU66_001705"
     CDS             1363..2235
                     /locus_tag="ACHU66_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR3389972.1"
                     /translation="MNQKGFIAKYWPYLFVAIPIGLQLIFFFYPLFTGIYYSLTDWNG
                     LTSDFKLIGIQNYLDILKNPDFYTSMTFTIIFTIGLVIGEIVIGIWLASLLNRKIKAV
                     GFFRTWYFFPAVLSTVTLGLIFVQLFNYGFTQIGEILHIDWLMENLLVQENTVIPAVL
                     FVALWQGLAMPVIIFLSGLQSIPEDVKEAAAIDGATRSQQFFNIELPFLLPSISMVFI
                     LAMKSGLTAFDLIFALTSGGPDGKTESLGLLVYNYAFVDNKFSYANALAVVLFIFIIV
                     ISLIQMRISKKFEI"
     gene            2246..3076
                     /locus_tag="ACHU66_001706"
     CDS             2246..3076
                     /locus_tag="ACHU66_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR3389973.1"
                     /translation="MNTQKQKNWWVYLVLFSGILFMFIPLLVTIISSFKPTKEITSNF
                     FGLPENFTLNNYERLFNDGIVQYFGNSALITIVAVGLILLVIPMAAFAVARQMKRQTV
                     FNFIYFFLIIGIFVPFQVIMLPMTKLMSSLGLNNIVGLIILYLTYAVPQALFLYVGYI
                     KTIVPEEMDEAAAIDGCDKFTMYWKIIFPLMKPMHATVLIINALWVWNDFLLPLLVLN
                     RDQSMWTLPLFQYNYQGMYFSDYGPSFASYVVGIIPILLVYLIFQKHIISGMTSGSVK
                     "
     gene            3146..4594
                     /gene="gtfA"
                     /locus_tag="ACHU66_001707"
     CDS             3146..4594
                     /gene="gtfA"
                     /locus_tag="ACHU66_001707"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFR3389974.1"
                     /translation="MKIKNQAMLITYSDSLGKNLKDLKKVLEGPLKDVVGGIHILPFF
                     PSSGDRGFAPMDYTKVDPAFGDWSDIEALSKDYYLMFDFMINHISAKSPYFLDFLEKK
                     DESAYADLFIRYKNFWPNGEPTQEDVDLIYKRKPRAPYRIATFADGSEEKVWCTFDEQ
                     QIDLDVTTETTRRFIQENLEQLAEHGASIIRLDAFAYANKKIGTNCFFVEPDIWEMLH
                     FPRQLLAPKDVEILPEIHEHYTIQQKIAEQDYYVYDFALPMLVLHALYSGHVNRLVHW
                     MEICPRKQFTTLDTHDGIGVVDVKDLLTDEETEETREALYAQGANVKKIYSTEAYNNL
                     DIYQINCTYYSALGNNDQAYLLARVLQCFAPGIPQIYYVGLLAGENDIELLESSKEGR
                     NINRHYYDLEEIEQEVQRPVVQSLFKLLKFRNTSPAFDGEFSVKMLDETSMEIFWKNQ
                     DAGVSARLTANLKEKSFEIVEIEEGKITTIEL"
     gene            4667..6283
                     /locus_tag="ACHU66_001708"
     CDS             4667..6283
                     /locus_tag="ACHU66_001708"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR3389975.1"
                     /translation="MSEIQKTDWWKKSVIYQIYPRSFQDSNGDGVGDIRGIISRLDYL
                     HELGIDAIWLSPVYQSPMDDNGYDISDYQGIAPEFGTMEDMEELIAEGHKRNIKIIMD
                     LVLNHTSDEHFWFQEALKGPDNPYYDYYVWADEPNELRSTFSGSAWEYVPHLNKYYLH
                     QFSVKQPDLNWKNPALKQEIWDMINFWIEKGVGGFRLDVIDLIGKEPEKLITADGPTL
                     HPLIQELNEKTFGAYDLVTVGETWSANPENAQLYSHPDRKEFSMIFQFEHVGLDQQEG
                     KEKWDLAPLDPARLHKVLSKWQTELVGKGWNSLFWNNHDLPRAISRFGDDRPAFRELS
                     GKMLAIYLHFMSGTPYIYQGEEIGMINTPITDISQADDIETRRMYAERIENGFTKEEL
                     ITSINAKGRDNARRPMQWTAEEGAGFSTGQAWLALGDTVKDINVEKALADKQSLFYTY
                     QKIIALRKEYPCMSEGKYEVMETGNSSVMAYRKYDEQDDFIILVNFSSQEQAYTIAVE
                     EYSIFMNNYENEEAINKGMLRPFEAIVFKK"
     gene            6363..6992
                     /gene="upp"
                     /locus_tag="ACHU66_001709"
     CDS             6363..6992
                     /gene="upp"
                     /locus_tag="ACHU66_001709"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR3389976.1"
                     /translation="MGKFQVISHPLIQHKLSILRRTSTSTKDFRELVNEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQIAGKKLAIVPILRAGVGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRHIFVVDPMLATGGSAILAVDSLKKRGASNIKFVAL
                     VSAPEGVKALQDAHPDIDIYTAALDEKLNEKGYIVPGLGDAGDRLFGTK"
     gene            7094..7705
                     /locus_tag="ACHU66_001710"
     CDS             7094..7705
                     /locus_tag="ACHU66_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HFR3389977.1"
                     /translation="MSKRSNFMIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDN
                     MANSIIAQLLFLDAQDPTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGTAA
                     SMGTIIASSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNKLEKI
                     LADNSGKTVKQIHKDAERDYWMSAEETLAYGFIDQIMDNTKVK"
     gene            7832..8104
                     /locus_tag="ACHU66_001711"
     CDS             7832..8104
                     /locus_tag="ACHU66_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFR3389978.1"
                     /translation="MFEKKNRTCLTVYLHYNRDARKLSQYGDIVYHSKRLRYVLVYMD
                     QELVEATILKLKKERFVKKVVPSYIKELDQNFVGNLWRDEEPSVVG"
BASE COUNT         2498 a         1464 c         1680 g         2464 t
ORIGIN      
        1 gaaaacattt ttaaaatgtg catcggtttg tgcttttgct agcttcttgg ttgcttgtgg
       61 gaatgcaagt agtagcgata aggtagaaat tgaatatttc tcacaaaaac ctgaaatgca
      121 ggctactctt caggaaatta ttaatgattt cgaaaaggaa aatcctacga ttgatgttaa
      181 attttcaaat gtaccagatg caggaactgt tctgaaaacc cgtatggcta acaatgaagc
      241 accggatgtg atcaacattt atccacaaaa tgcggacttc aaagcatatg cagcggatgg
      301 acgtttccta gaaattggtg atgatgcagg tctcagtcat ctaaaagatg gtgctgtaac
      361 gccttatctt gttaacgaga aaaactatac tcttccattg acagctaatg cctatggtat
      421 ttactataat aaggataagt tcaaagaatt gggtctcgaa gttccaacta cctatgcaga
      481 atttgtggct ttggtagaca aaatcaaggc tgatggcagt gcagcaccgt ttgctctttc
      541 attgaacgat gcttggtcat tgaacggcta ccatcagtta gcttgggtaa ctgttgcggg
      601 tggatttgat ggtgcagaag atattctgat tcgtagtgca aagggagcga ttcaagatga
      661 tgcgacaaca aaagcagtgt tagaacgctt gcaattgttg aaagacaatg gacaaaaagg
      721 ggcaacaggc gcgctctatg cagatgcagt ggcatccttt gcagcaggtg atgctctcat
      781 gcttccacaa ggtacatggg cagctacagc tgtaaaccaa caagaaccag aatttgaata
      841 tggtatgttt accttccctg gtgataaaga tggtggtgac tataccatcg gtgcagcaga
      901 ccttgctcta tctatttctg cagatacaga gcacccagaa gagtctaaaa aattcctaga
      961 atatctctct cgtccagaag tgatgcaaaa atactatgat gtagatggct caccaacttc
     1021 agttgaaggt gtagatacag aaggtaagtt tgaagaaact gctggagtta cacaatatgc
     1081 ctttactgat aaacacgtag tttggttgca atctgaatgg gaatcagaag aagagttctg
     1141 gaacatcact gttgaaatgg taaaaaatcc aaactcagca gaattagtga aaaaactgaa
     1201 cgcattcttt gatccaatga aaaaataatc caggaaaact ataagcaggg gctagttgta
     1261 gcctagcctc cttatagttg cctagtgtca gttttagtaa ccctatggga ttccttgaac
     1321 tgagactaag caactataac tttatcaaaa aggagtacca tgatgaatca aaaaggtttt
     1381 attgccaaat attggcccta tctatttgtt gccataccaa ttggcttaca gttaatcttt
     1441 ttcttctatc ccctatttac aggtatctat tatagcttga cggattggaa tggattgaca
     1501 tctgatttca aactaatcgg tattcagaat tacttagata ttttaaaaaa tcctgatttt
     1561 tacacttcca tgacttttac cattatcttc accattggtt tggtcatcgg agaaattgtt
     1621 atagggattt ggttggctag tctcctcaat cgtaagatta aagcagttgg tttctttaga
     1681 acttggtatt tcttcccagc agtactatcc acagtcactt taggattgat ttttgttcaa
     1741 ttgttcaact atggttttac acagattgga gaaatccttc atatcgattg gttgatggaa
     1801 aacttattgg tacaagaaaa tactgttatt ccagctgtac tttttgtggc tctctggcaa
     1861 ggattggcta tgccagttat catcttcctg tctggcttac aaagtattcc agaagatgtg
     1921 aaggaagcag ctgcaatcga tggtgcaaca cgctctcaac aattctttaa tattgaactt
     1981 ccattcttat taccatctat cagtatggtt ttcatcttag caatgaagtc aggtttgaca
     2041 gcattcgacc ttatctttgc attgacaagt ggcggcccag atggcaaaac agaatccttg
     2101 ggtttactcg tttacaacta tgcctttgta gacaataaat tctcttatgc caatgctttg
     2161 gctgtagtac tcttcatctt cattattgtc atctcattga ttcagatgag aatttcgaag
     2221 aaatttgaaa tttaggagga ctgtcatgaa tactcaaaaa cagaaaaatt ggtgggtcta
     2281 tctcgtactc ttcagtggta ttctcttcat gtttattccg cttcttgtga cgattataag
     2341 ttcgttcaaa ccaaccaagg aaattaccag caacttcttt ggtcttcctg aaaatttcac
     2401 cttaaataac tacgaacgct tgtttaatga cgggattgta caatattttg gcaattctgc
     2461 cttgattacg attgtagctg ttggcttgat tctacttgtt attccaatgg cagctttcgc
     2521 cgtagcccgt caaatgaaac gtcagacagt gtttaacttt atctactttt tcttgattat
     2581 tgggattttt gtacctttcc aagtgattat gctcccaatg accaaattaa tgtcaagcct
     2641 tggtttgaac aatattgtcg gtttgattat tctatatttg acctatgcag ttcctcaggc
     2701 cctcttcctc tacgtcggtt atattaagac cattgttcct gaagaaatgg atgaagcggc
     2761 agctattgat ggctgtgata aatttaccat gtactggaaa atcattttcc cactcatgaa
     2821 acccatgcat gctacggttt tgattatcaa tgccctctgg gtctggaacg attttctatt
     2881 accgctcttg gttttgaatc gtgatcaaag catgtggact ttacctcttt tccaatacaa
     2941 ctatcaaggt atgtatttca gtgattatgg accatcattt gcttcctatg ttgtaggaat
     3001 tatcccaatc ttacttgttt acctcatctt ccaaaaacat attatttcag gtatgacgag
     3061 tggttcagtc aaataaatct aaaggttgac ttgttcaacc ttttctaaaa aataaaaaag
     3121 aaaaggctta caaaggagag tatcgatgaa aattaaaaac caagctatgt tgattactta
     3181 ctcagacagt ttgggtaaga atctcaagga tttgaaaaaa gttcttgagg gaccattaaa
     3241 ggatgttgtg ggaggtattc acattctacc attcttccca tcatcaggtg accgtgggtt
     3301 tgcaccaatg gattatacaa aggtagatcc tgcatttgga gattggtcag atattgaagc
     3361 attaagcaaa gactattatc tgatgttcga ttttatgatc aatcatattt ctgcaaaatc
     3421 tccttatttc cttgattttc ttgaaaagaa agatgaatca gcttatgcgg atttgtttat
     3481 tcgctataaa aacttttggc caaatggtga gccaacgcaa gaagatgttg atttgatcta
     3541 taagcgtaaa cctcgcgctc catatcgaat tgcaacattt gctgatggta gcgaggagaa
     3601 ggtatggtgt acctttgatg agcagcagat tgacttggat gtgactacag agacaacacg
     3661 ccggttcatc caagaaaatc tggaacagtt ggcagaacat ggtgcttcga tcattcgttt
     3721 ggacgccttc gcttatgcca ataaaaaaat aggaaccaac tgtttctttg tggagcctga
     3781 tatttgggaa atgctccatt ttccacgtca gttattggca ccaaaagatg tggaaatctt
     3841 gcctgaaatc catgaacatt acaccattca gcaaaaaatt gctgaacaag attattatgt
     3901 ttatgacttt gctctgccaa tgctggttct ccacgctttg tattccggtc atgtcaatcg
     3961 cctggtacac tggatggaga tttgccctcg caagcaattt acaaccctag atacacatga
     4021 tggaatcggt gttgtagacg ttaaggattt gctgacggat gaagagacag aggaaacgcg
     4081 tgaagccttg tatgcacaag gtgccaatgt gaagaaaatt tatagtacag aagcctataa
     4141 taatttggat atttatcaga ttaactgtac ttattattct gcccttggca ataacgatca
     4201 agcttaccta ttagcgcgtg ttctccaatg ttttgcacca ggtattccac aaatttacta
     4261 tgttggtttg ctagcaggcg aaaatgatat tgagcttttg gaatcaagca aggaaggccg
     4321 caatatcaat cgccattact atgatttaga agaaattgag caggaagtac aacgccctgt
     4381 ggtacaatcc ttgttcaaac tccttaaatt ccgcaataca agtccagctt tcgacggaga
     4441 gttctctgtt aagatgctgg atgaaacaag tatggaaatt ttctggaaga atcaagatgc
     4501 aggggtgtct gcccgtctaa cagcaaacct aaaagaaaaa tcctttgaaa ttgttgaaat
     4561 tgaagaggga aaaattacca caattgagtt ataatatgat tatatagaaa gagggggatt
     4621 cctctttttc ttgactaaat aagattgtgc agaaaggatc aatcaaatgt cagaaataca
     4681 aaagacggat tggtggaaga agtccgtcat ttaccaaatt tatcctcgta gttttcaaga
     4741 ttcaaatgga gatggagtcg gggatattcg agggattatc agccgattag attatctcca
     4801 tgagcttgga attgatgcta tttggctcag tcctgtttat cagtccccta tggatgataa
     4861 tggttacgac atcagtgact accaaggtat tgctccagaa tttgggacca tggaagatat
     4921 ggaagagctg attgcagaag gccataagcg caatatcaaa ataatcatgg atttagtgct
     4981 caatcatacc tcggatgaac atttctggtt tcaagaagct ctcaaaggtc cagataatcc
     5041 ctattatgat tattatgttt gggctgatga gccgaatgaa ttacgctcga ccttctcagg
     5101 ttctgcttgg gaatacgttc cacatctgaa caaatactac ctccaccaat tctctgttaa
     5161 gcaaccagat ctgaactgga aaaatcctgc tctgaaacaa gaaatctggg atatgatcaa
     5221 tttttggatt gaaaaaggtg tcggcggttt caggctagat gtgattgatt tgattggaaa
     5281 agaaccggaa aaattaatta cagctgatgg tcctactctc catcctctta ttcaggaatt
     5341 aaatgagaaa acctttggtg catacgactt agtgactgtt ggggagacat ggagtgccaa
     5401 tccagaaaat gctcagttat attcgcatcc tgatcgaaaa gaattttcga tgattttcca
     5461 atttgaacat gtaggacttg atcagcagga agggaaagaa aagtgggatt tggcaccgct
     5521 tgatccagct cgtttgcata aggttctatc caaatggcag actgaactgg ttggtaaagg
     5581 ctggaactcg cttttctgga ataaccatga tttacctcgt gccatttctc gcttcggtga
     5641 tgatcgacct gcatttcgtg aactaagtgg taaaatgtta gctatttatc ttcattttat
     5701 gtcagggaca ccttatatct accaaggtga ggaaattggt atgattaaca caccaattac
     5761 tgatattagc caagcggacg atattgaaac acgtcgcatg tatgcggaac ggattgaaaa
     5821 tggcttcacc aaggaggaat tgattacttc tataaatgcc aaaggtcgtg ataatgctcg
     5881 ccgtccaatg caatggacag cagaagaagg tgcaggtttc agtacaggtc aagcctggct
     5941 ggctttggga gatactgtca aagacattaa cgttgaaaaa gcacttgcag acaaacaatc
     6001 gcttttctat acctatcaaa aaataattgc tcttcggaaa gagtatcctt gtatgtctga
     6061 aggaaaatat gaagtgatgg agactggaaa tagctcggta atggcctacc gtaaatatga
     6121 tgaacaggat gatttcatca tattagtaaa cttttcaagc caggagcagg cttatactat
     6181 agctgtggaa gaatattcta ttttcatgaa caattatgaa aacgaagagg cgattaataa
     6241 agggatgttg agaccttttg aagctatcgt tttcaaaaaa taaataaatt tgccatattc
     6301 tcaagcattt tttcaatttt tttggtaaaa tagaaaaaac aaaaaagaga ggttttatca
     6361 tcatggggaa attccaagtc atttcacacc cactcattca gcataagttg tctatccttc
     6421 gtcgtacatc tacttcaaca aaggattttc gtgagttggt taatgaaatc gcgatgctga
     6481 tgggttacga agttttgcgt gacttgccac ttgaagatgt ggagattgaa acgccaatca
     6541 ctaaaacagt tcagaaacaa attgcaggta aaaaattagc tatcgtacca attcttcgtg
     6601 caggtgttgg tatggttgat ggtttgctaa gcttggtacc agcagctaaa gttggtcaca
     6661 tcggcatgta ccgcgatgaa gaaacccttc aacccgttga gtatcttgtg aaattaccag
     6721 aagacattga tcaacgtcat atttttgtag ttgacccaat gcttgctaca ggtggttcag
     6781 ctattcttgc agttgattca ttgaaaaaac gtggtgcttc aaatatcaaa tttgttgctc
     6841 ttgtttcagc tccagaaggt gtaaaagctc ttcaagatgc acatccagac attgatattt
     6901 atacagcagc tttggatgaa aaactcaatg aaaaaggcta catcgtacca ggtttgggag
     6961 atgcaggtga ccgcttgttc ggtacaaaat aatttataga aaggaatcag cttgctggtt
     7021 tcttttttcg aatgtgaaaa tcgtgttttt tatttgacca attttgacta ttcatatata
     7081 atgggtacag aaaatctcga aaaggagtaa ttttatgatt ccagtagtta ttgaacaaac
     7141 tagccgtggt gagcgttctt atgatattta ctcacgcctt ttgaaagacc gcattatcat
     7201 gttgacagga ccagttgagg acaacatggc aaactctatc attgcacaat tgcttttcct
     7261 tgatgcccaa gacccaacca aagatattta cctttatgta aatacacctg gtggatctgt
     7321 gtcagctggt ctagctattg tagatactat gaattttatc aaggctgatg tacaaacaat
     7381 cgtcatgggg acagctgcca gcatgggtac aattatcgct tcaagtggtg ccaagggcaa
     7441 acgtttcatg ttaccaaatg cagagtacat gattcaccag ccaatgggtg gaactggtgg
     7501 cggtactcag caaacagata tggctattgc agcagaacac ctattaaaaa cacgtaataa
     7561 gctagaaaaa atcttggcag acaactcagg taagacagtc aagcaaatcc acaaggatgc
     7621 ggaacgcgat tactggatgt cagctgaaga aaccttggct tacggattta ttgaccagat
     7681 tatggacaat acaaaagtca aataagcagt gacaaatatg taatagtact gactattcct
     7741 attagctcca actcttttac aggtagttgg agcttttcat ttatcattga aaacggtata
     7801 atatagaaag agaattttta gaggagaaga catgtttgag aagaaaaatc gcacttgctt
     7861 aactgtctat ttacactaca atcgtgatgc acgtaaactc agccaatatg gagatattgt
     7921 ttaccattcc aaacgcttgc gatatgtttt ggtgtatatg gatcaagaac tagtagaagc
     7981 taccatatta aaattgaaaa aggaacgttt tgtaaaaaaa gtggttcctt catacattaa
     8041 ggaattagac caaaactttg taggtaatct atggagagat gaagaaccat cagtcgtagg
     8101 atagtc
//
LOCUS       DAZQCJ010000089         7966 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000089 DAZQCJ010000000
VERSION     DAZQCJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.090"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            66..536
                     /locus_tag="ACHU66_001712"
     CDS             66..536
                     /locus_tag="ACHU66_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFR3389979.1"
                     /translation="MKLQNLQVGQSYLVKDCLTQDDIRKHLAHLGLKVGEEIRIISKT
                     KTNAIFQVKASRLALDREIIESLVLTEKSATEIINLSEAPIGSSAKVMDIYATGALRR
                     RLMDMGLTKNTQLFLKKVAPLGDPIEITLRGYELTLRKSEAQMIGVQITSEVRK"
     gene            533..2677
                     /gene="feoB"
                     /locus_tag="ACHU66_001713"
     CDS             533..2677
                     /gene="feoB"
                     /locus_tag="ACHU66_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFR3389980.1"
                     /translation="MTKHIALAGNPNSGKTTLFNLLTGTNQRVGNWPGVTVERKSGTI
                     KKRKHLLIQDLPGIYSLSPYTPEERVARDYLIADQPAAILNVLDATNLERNLYLTLQL
                     LEMGLPIAIALNMTDALKSQGRSINSDQLSYQLGVPVQPISALKKLGISQLLHEVEKI
                     TNQQAEPIYPQYDKQFEAGLAQVIDLLSDSIPNHQKRFYAIKLLEQDQVILDQLQLNA
                     QDMLDLTEIIAILEKIYADDMEAIIVNQRYQFIEKITELVTKDSDKAFNLSDNIDRLV
                     TNRFLALPIFAAVMWLTYFLSIQTVGTMGTDWVNDVLFGELVPGLIQANLDRFEIAGW
                     LQSLVLDGIIAGCGAILGFVPQIFVLFVCLGILEDIGYMSRVAFVMDRIFRRFGLSGK
                     SFIPMLISTGCGVPGVMASRTIENEQDRKITIMTATFMPCSAKLPIISLVAGAFFPDN
                     PWIAPSAYFVGMAAIVLSGMALKKTKQLGGVASPFIMELPSYHLPKLSTVLRYAFDKA
                     LSFIKRAGTIIFVTNIIIWFSSSYNWSLQMVETDESILASIGHSFSLLFAPLGFENWR
                     ATVAAITGLLAKETVIATFGILYKLGETTEENPELWGLLQQDYTALSAYSFLVFNLLC
                     APCFAAIGAIHREMGEAKWTWIAIGFQTGLAYASSLVIYQIGLVLIHGQLPTVWTFIA
                     IFLIITAIYSLVKKPTNTLPIVTLKTLEKGEY"
     gene            2677..2829
                     /locus_tag="ACHU66_001714"
     CDS             2677..2829
                     /locus_tag="ACHU66_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFR3389981.1"
                     /translation="MPTLILFLIIVILFSLAIRSLIKGKGSCGDCSCDCTIKQEMNES
                     SHHRHP"
     gene            complement(2882..3811)
                     /gene="ftsX"
                     /locus_tag="ACHU66_001715"
     CDS             complement(2882..3811)
                     /gene="ftsX"
                     /locus_tag="ACHU66_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR3389982.1"
                     /translation="MSNRFFRHFIESLKSLKRNGWMTIAAISSVAITLTLVGLFASVI
                     LNTAKLASDLEQNVRINVYLRANSTDQAETIVNEAGKTVANPDYKKVYNQITALENVQ
                     SVTYSSKDEQLQKLTATLGDTWNLFQGDANPLYDAYIIDTTEPQYVKTVAAEIAKIDG
                     VTEVRDGEVETERIFKLANLVRTWGLAATGLLLFTAVFLISNTIRITIISRSREIQIM
                     RLVGAKNSYIRGPFLWEGAWVGLLGAILPSALVYSFYKMIYTSVNASLASQDLSLISM
                     DVFVPGMIGALFVIGIIIGSLGSVISMNRYLKI"
     gene            complement(3804..4496)
                     /gene="ftsE"
                     /locus_tag="ACHU66_001716"
     CDS             complement(3804..4496)
                     /gene="ftsE"
                     /locus_tag="ACHU66_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR3389983.1"
                     /translation="MGIIEMKDVSKKYGNGTTALRGVSVNVEAGEFAYIVGPSGAGKS
                     TFIKLLYREEKLDKGSLKVGKFDLAKIKKRDVPLLRRSVGVVFQDYKLLPKKTVFENI
                     AYAMEVIGEKPRNIKKRVMEVLDLVGLKHKIRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPENSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIEDGRVV
                     RDEAEGEYGYDE"
     gene            complement(4639..5734)
                     /gene="prfB"
                     /locus_tag="ACHU66_001717"
     CDS             complement(join(4639..5661,5663..5734))
                     /gene="prfB"
                     /locus_tag="ACHU66_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775232.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR3389984.1"
                     /translation="MDTAEIRQKIEELGKKLTSFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNLAAQKTSQELNELKNTYGNFHQMLDLYDESEILLDFLAEDESVRDELVEKLVE
                     LDKTMTAYEMTLLLSDPYDHNNAILEIHPGSGGTEAQDWGEMLLRMYQRYGNAKGFIV
                     ETLDYQAGDEAGIKSVTLSFTGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEIEIRDDEVKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVTQSTVDRT
                     QYGNRDRAMKLLQAKLYQLEQEKKAAEVDSLKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTGYEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            complement(5769..6896)
                     /gene="queG"
                     /locus_tag="ACHU66_001718"
     CDS             complement(5769..6896)
                     /gene="queG"
                     /locus_tag="ACHU66_001718"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR3389985.1"
                     /translation="MNIKEEIIKLSQEIGISKIGFTTADDFAYLEKSLRASQEEGRSS
                     GFEHKNIEERIRPKLSLESAKTIISIAVAYPRHLPVKPQKTQYKRGKITPSSWGLDYH
                     YILQDKMERLARGIERLTDGLEYKAMVDTGALVDTAVARRAGIGFIGKNGLVISKEFG
                     SYMFLGELVTNLDIEPDQPVDYDCGDCNRCVAACPTSCLLGDTTMNARRCLSFQTQDK
                     GMMDLEFRKKIKTVIYGCDICQICCPYNKGISSPPVVEIDPDLAEPELIPFLDLSNGQ
                     FKEKFGMIAGSWRGKNILQRNAIIALANSNDRSAIPKLLEIIDKKQNPVHMATAIWAL
                     GQLVKQPNDEMVSFIAGISSDNDDVIAEQAAFLNMVKDLQM"
     gene            complement(6937..7425)
                     /locus_tag="ACHU66_001719"
     CDS             complement(6937..7425)
                     /locus_tag="ACHU66_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFR3389986.1"
                     /translation="MQTKKMSMFLFFAYLLLLTWMIIFKMDLSIVYGRYGYASINLIP
                     FAGTAVYDGVLDFPEILFNIVSFIPFGIYMEMLFRKASWVTNLCLIMLVSLCFEVLQY
                     LLLLGVADITDLLANGLGGAIGINIMYVLTSIWREKAYVRMNIFCFVLTFFVILITYL
                     AM"
     gene            complement(7595..>7966)
                     /locus_tag="ACHU66_001720"
     CDS             complement(7595..>7966)
                     /locus_tag="ACHU66_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HFR3389987.1"
                     /translation="PSDKLFILQKELEDLDDEALNVLMMVEMRQPLVALILAIFFGEF
                     GVDRFYVGNKELGFAKLIAFAVSFVTLFILIGFLLFLGLYLWKFIDCFLIMRACKEAN
                     FERLMLQIHQYKAFQHSNQTF"
BASE COUNT         2443 a         1878 c         1381 g         2264 t
ORIGIN      
        1 aaattgtctg acacttttaa ggaattgtgc tatactattg ttataaaaat cacaaaggaa
       61 acgatatgaa actgcaaaac cttcaagttg gacaatctta tttagtcaaa gattgcctaa
      121 cgcaagatga tattcgtaaa cacttagcgc acttgggttt aaaagtcgga gaagagattc
      181 gcatcatttc aaaaaccaag accaatgcaa ttttccaagt caaggcaagc cgtctagccc
      241 tagacagaga aattattgaa tctctggttt taacagaaaa gtcagctacg gaaataatca
      301 atctatctga ggcacctatt ggtagctcag ccaaggtcat ggacatctat gcaacaggtg
      361 cactccgtcg tcgcctcatg gatatgggac ttacaaagaa cacacagcta tttctcaaaa
      421 aagtcgctcc cctaggcgat ccaatagaaa ttaccttgag aggatacgag ctgacactac
      481 gcaaatccga agcgcaaatg attggtgttc aaatcacaag cgaggtgagg aaatgacaaa
      541 acacatcgcc cttgcaggaa atccaaatag cggaaaaaca actcttttca acctcctaac
      601 aggaaccaac caacgtgttg gtaactggcc cggagtcacc gttgaaagaa aatctggaac
      661 catcaaaaag cgaaaacatt tactaattca agatttacca ggtatctact cgctgtctcc
      721 ctacacacca gaagaacggg ttgccagaga ttatttgata gcggaccaac cagctgctat
      781 cctcaacgtc cttgatgcaa caaacttgga gcggaatctc tatctgacct tgcagttatt
      841 agaaatgggc cttcctatag ctatcgccct caatatgaca gacgccctga aaagtcaagg
      901 tcgctcaata aacagcgacc agctctccta tcaactggga gttcctgtac agcctatcag
      961 tgcactaaaa aaactaggta tatcccagct cctccatgaa gtagaaaaaa ttaccaatca
     1021 gcaagccgag ccaatctatc ctcagtatga taagcaattt gaagccggtc ttgcccaagt
     1081 tattgaccta ttatcggatt ccatcccaaa tcatcaaaaa cgcttctacg ccattaagct
     1141 actcgaacaa gatcaagtca ttctagacca attgcaacta aatgcacaag acatgcttga
     1201 tttgaccgaa atcattgcca ttttggaaaa aatttacgca gacgatatgg aagccattat
     1261 cgtcaatcaa cgctaccaat ttatcgagaa aatcacagaa ctggtcacaa aagacagcga
     1321 taaggctttt aacctgtctg ataacataga ccgacttgtt accaatcgtt ttcttgccct
     1381 ccctatcttt gcggcagtca tgtggctgac ctacttcctg tctattcaaa cggtaggtac
     1441 aatggggact gactgggtga atgatgtcct cttcggagag cttgttccag gccttattca
     1501 ggcaaatcta gatcgatttg aaatcgctgg ctggctacaa tctctggtcc ttgatggcat
     1561 tattgcagga tgcggagcca ttctaggttt tgtaccacaa attttcgtcc tcttcgtctg
     1621 cttgggaatc ttggaagaca tcggctatat gagccgagtc gcctttgtca tggataggat
     1681 ttttagacgt tttggtctat caggcaaatc ctttattccc atgttgattt ccacaggctg
     1741 tggcgttcct ggagtcatgg caagccggac tattgaaaat gaacaggacc gaaaaatcac
     1801 cattatgaca gcaaccttca tgccctgttc tgccaaactt cctatcattt cactggtagc
     1861 cggtgctttc tttcctgaca atccttggat tgctccaagc gcctattttg tcggtatggc
     1921 tgccatcgtc ctatccggaa tggcactaaa aaagaccaag caacttggtg gagtcgcaag
     1981 cccctttatc atggaattac catcttatca cttaccaaaa ctcagtacgg ttctccggta
     2041 tgcattcgat aaggcactta gttttatcaa acgagctggt accattatat ttgtgaccaa
     2101 catcattatt tggttcagca gttcctataa ttggtcttta caaatggtag aaacagatga
     2161 gagtatctta gcctccattg gacacagttt ttctctcctc tttgccccac ttggtttcga
     2221 aaattggcga gcaactgttg cagccattac aggtctctta gccaaggaaa ccgtcattgc
     2281 cacctttggt atcctctata aattaggaga aacaaccgaa gaaaatcccg aactatgggg
     2341 gcttttacaa caagactata cagcgttatc agcctactcc ttcctagtct tcaatctcct
     2401 ctgtgcccct tgttttgcgg ctattggtgc cattcaccgt gaaatgggag aagccaagtg
     2461 gacatggatt gctatcggtt tccaaacggg tttagcctat gcaagtagtt tggttatcta
     2521 tcagattggt cttgtactta tccacggaca attaccaacc gtctggacat ttatcgctat
     2581 ctttctaatt atcacagcca tctacagcct agtgaaaaaa ccaaccaaca ctcttccaat
     2641 cgtcacgcta aaaacattag aaaaaggaga atattaatgc ccacacttat cctctttctc
     2701 atcatcgtca tcttattttc tcttgcgatt cgcagtctca tcaaaggaaa aggatcctgc
     2761 ggagattgtt cctgcgactg tactattaag caagagatga acgaatcttc tcaccataga
     2821 catccataac aaaaaggtct gcctcattat tgtgcagacc tgtttacatg tagcttgtct
     2881 attaaatttt taagtagcgg ttcatggaaa tgactgatcc caaggaacca ataataatac
     2941 caattacaaa aagtgcgcca atcattcctg gaacaaagac atccatacta atcagagaca
     3001 aatcttgact agcaagacta gcatttaccg aagtataaat catcttataa aaagaataga
     3061 ctaaagcaga aggcaaaata gctccgagga gacctaccca ggcaccttcc cagaggaatg
     3121 gtccacggat ataactgttt ttagcaccaa ccagacgcat aatctgaatt tcacggctac
     3181 gagaaataat ggtaattcga atagtattgg aaatcaagaa aacagcagta aagagcaata
     3241 agccagttgc tgccaatccc catgtacgaa ctagattagc taatttaaaa atacgttccg
     3301 tttcaacttc cccatcccgt acctctgtaa caccatcaat tttagcaatc tctgctgcaa
     3361 cagtttttac atattgcggt tcagtcgtat caatgatata agcatcgtaa agtggattgg
     3421 catctccttg gaataggttc caagtatctc ccaaggtagc tgttaatttc tgtaactgtt
     3481 catccttact tgaataagtg acagattgta cattttctaa ggctgtaatc tgattataca
     3541 cctttttata atctggatta gctaccgtct ttcctgcttc gttcacaatc gtctcagctt
     3601 gatcggttga gttcgctctc aaatacacat tgatgcgtac attctgttcc aaatccgaag
     3661 cgagctttgc tgtgttcaaa ataactgaag caaatagacc aaccaaggta agggtaatgg
     3721 ctacagatga aatggctgca atcgtcatcc aaccatttcg cttcaaactc ttcaatgact
     3781 caataaagtg tctaaaaaat cgattactca tcgtatccat attctccttc cgcttcatca
     3841 cgtactacac gaccatcttc aatcgcaata acacgatgac gaagtgtatt tacaatctga
     3901 ctattgtgtg tcgccatcaa aatcgttgtc ccctgtaagt taattcgctc caatagattc
     3961 atgatttccc aagagttttc agggtccaag ttaccagttg gttcatcagc aatcaatact
     4021 ttaggattat ttacaatggc acgggcaata gcaatccgct gttgctcacc acctgaaagt
     4081 tcatttggga aagaacgaat tttgtgcttc aaaccaacca aatctaaaac ttccattaca
     4141 cgtttcttga tattccgtgg tttctcaccg ataacttcca tcgcataggc aatattttca
     4201 aagacagtct ttttaggtaa gagtttgtaa tcttggaaga ccactcctac actacgacgt
     4261 agaagaggaa catctctctt tttaattttt gcaaggtcaa acttaccaac ctttagactc
     4321 cccttatcga gcttttcctc ccgatacaag agtttgataa aggttgactt acctgcacca
     4381 gaaggaccta caatgtaggc aaattctccc gcttcaacat tgactgatac tccgcgaagg
     4441 gcagtcgttc cgtttccata tttcttggaa acgtctttca tttctattat tcccatagga
     4501 tttcctgtct tagacagtcc tttctttaga tttgagaacg tttttttcaa aatacagtcc
     4561 gtattctcta aaaaaacttc cttgatgagc gaaaaactat ctcttattta caagcacttc
     4621 acttgtaaat acaggttctt agctaattcg ccattttaaa taagcatcta taaagccgtc
     4681 caagtctccg tccataacct tatcgacttg agccacctca taaccagtac ggtggtcttt
     4741 taccattgta taaggtgtaa agacataaga acgaatctga cttccccaag tgatctcttt
     4801 cttgtcaccc ttaagtgagt ctacttctgc cgctttcttc tcctgctcca attgatacaa
     4861 tttagcctgc aagagtttca ttgcacggtc tcggtttcca tactgagtac ggtcaactgt
     4921 ggactgagtc acaatccctg tcggaatgtg cgtcaatcga acacccgttg aaaccttatt
     4981 gacgttctgt ccaccagcac ctcctgaccg gaaggtatcc atcttcactt catcatctct
     5041 gatttcaatt tcaatggtat catccaactc aggcattacc tccacagatg taaaggaagt
     5101 atgacgacgc ttagctgagt caaaaggtga gatgcgcacc aaacgatgaa cacccatttc
     5161 tgacttaagc aagccatagg cgtttggacc agtaaagctt agagtcacag acttaatacc
     5221 tgcctcatca ccagcttgat aatccaatgt ttctactata aaacctttgg catttccata
     5281 acgctgatac atccgcagga gcatttctcc ccagtcttgt gcctcggtac cacccgaacc
     5341 tggatggatt tccaagatag cgttattgtg atcatagggg tctgataaga gcaaggtcat
     5401 ttcgtaagca gtcatggttt tgtccaattc cactaacttt tcaaccaact catcccgcac
     5461 agactcatct tctgctagaa aatctagcaa aatttcagat tcatcatata aatccaacat
     5521 ctgatggaaa tttccataag tattttttaa ttcattcaac tcctgagacg tcttctgggc
     5581 tgccaaatta tcattccaga aatctggctc cgtcatcttg ttctcaagaa tggcaatctc
     5641 ttcttccaga ccttctaagt caaagagacc ccctaaaaga agttaatttt ttgccaagtt
     5701 cttcaatctt ttggcgaatt tctgctgtat ccatatacac ctcattttta ttcataatct
     5761 ttattatatc acatttgcaa gtccttgacc atgttgagaa aggccgcttg ctctgctatg
     5821 acatcatcat tgtctgatga aatacctgcg ataaaggaaa ccatctcatc attgggctgt
     5881 ttgaccaatt gccccaaggc ccagatagcc gttgccatgt ggaccggatt ttgtttttta
     5941 tcgatgattt ctaggagttt gggaatggca gagcgatcat tactattagc caaggctatg
     6001 atggcattac gctggagaat atttttcccc cgccaagaac ctgcaatcat accaaatttt
     6061 tccttgaatt gtccatttga caagtccaag aatggaatca gctcaggctc agccaaatca
     6121 gggtcaatct ccaccacagg tggactagaa attcccttgt tgtaaggaca acaaatctgg
     6181 caaatatcac aaccatagat gaccgtcttg attttcttac gaaactccaa gtccatcata
     6241 cccttatcct gggtctgaaa ggacagacag cgacgggcat tcatggtcgt gtcacctaat
     6301 agacaagacg tcggacaggc tgcaacacag cgattgcaat caccacaatc ataatctact
     6361 ggttgatctg gttcgatgtc caggttggtc actaattctc ctagaaacat ataggaacca
     6421 aattccttag aaatgactag cccattctta ccgataaagc caattcctgc tcttctggca
     6481 acagctgtat ccaccaaagc tcctgtatct accatagcct tgtattccaa accatccgtc
     6541 agtctctcaa ttccccgtgc caaacgctcc atcttatctt gcaagatata gtgataatca
     6601 agcccccatg aactaggggt aatcttccca cgtttgtatt gggtcttctg cggtttaaca
     6661 ggcaaatgtc gaggataagc taccgcgata gaaatgatgg ttttggcaga ttctaacgac
     6721 agtttgggac ggatacgttc ttcaatattc ttgtgttcaa atcctgacga acgcccttct
     6781 tcctgactag cacgaagtga tttctccaaa taagcaaaat catcagccgt tgtaaaacca
     6841 atcttcgaga ttccgatttc ctgagataat ttaataatct cttccttaat attcattata
     6901 ttcattttag caaactttga atatatttag caagacctac atggctaaat aagttattaa
     6961 tataacaaaa aacgttagca caaaacaaaa aatattcata cgaacatagg ccttctcccg
     7021 ccaaatactg gtcaagacat acatgatatt aataccgatt gctcccccta agccgttagc
     7081 cagtaaatca gttatatctg caaccccaag taaaagaaga tattgcagaa cctcaaagca
     7141 gagactcact aacataatca aacatagatt agtcacccac gatgccttgc gaaatagcat
     7201 ttccatataa ataccaaagg gaataaaact tacaatatta aatagaattt ctggaaaatc
     7261 cagcactcca tcatagaccg ctgttcctgc aaacggaatt aagtttatac tagcataacc
     7321 atagcgtcca tatacgatgc tcaggtccat cttaaagata atcatccaag taagtaaaag
     7381 gagataggca aaaaataaaa acatactcat tttctttgtc tgcatagaaa ttctcctctc
     7441 aactagcata ttttatcatt atatcataaa tgctgttcta taaaatgtca cttctgtgac
     7501 aaagctcgtt ccatattaca agtactttac agcctactaa aaacagaaaa ggaagacact
     7561 accaaacctc cccttattcc caaaaatatc ttacttaaaa tgtctgattg ctatgctgaa
     7621 aagccttata ttgatggatt tgtagcatca aacgctcaaa gttcgcttcc ttacatgctc
     7681 gcatgatcaa gaagcaatca ataaacttcc acaaataaag tcccaaaaat aacaagaacc
     7741 caatcaagat aaatagcgta acaaaagata ctgcaaaagc aatcagcttg gcaaatccca
     7801 attctttatt cccgacataa aaacggtcga caccaaactc accgaagaaa atagccaaaa
     7861 taagagcgac aagaggttga cgcatctcaa ccatcataag aacgtttaaa gcctcatcat
     7921 ctaaatcttc tagttctttt tgcagaatga ataacttatc agatgg
//
LOCUS       DAZQCJ010000090         7897 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000090 DAZQCJ010000000
VERSION     DAZQCJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.091"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..492
                     /locus_tag="ACHU66_001721"
     CDS             <1..492
                     /locus_tag="ACHU66_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFR3389988.1"
                     /translation="DTQKFKLGMRTLKSGLAVFLVILLFHLLKWDGIQIAALSAVFSL
                     REDFGKSVHFGSSRVLSNSIGGFYALLYFILSDLFSHNFWVTLIFIPIFTMLTIMTNV
                     SFNNAPGVISGVATMLIIALSVPAGDSIFYALQRVFETFVGVFVSVIVNYDIDKIKKR
                     FLS"
     gene            572..943
                     /locus_tag="ACHU66_001722"
     CDS             572..943
                     /locus_tag="ACHU66_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFR3389989.1"
                     /translation="MREKEFRRSLAIFPIGSVMKLTDLTARQIRYYEDHGLIKPVRNE
                     GNRRLYSLNDMDLLLEIKDFLDEGLNIAAIKKEYANRKAKEKVQAEQKTLTDEDVRRI
                     LHDEFSRQGRFSSPSSIFRSS"
     gene            970..2316
                     /gene="glnA"
                     /locus_tag="ACHU66_001723"
     CDS             970..2316
                     /gene="glnA"
                     /locus_tag="ACHU66_001723"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFR3389990.1"
                     /translation="MVVTTADIKRDIKEKNVTFLRLMFTDIMGVMKNVEIPATEEQVE
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLNTWTIFPWGDENGRVAGLICDIYTTEG
                     EPFAGDPRGNLKKSLRRMEKEGFSSFNLGPEPEFFLFKMDEQGNPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTEMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDKDGNNAFFDPEDPRGMQLSETAY
                     HFLGGLIKHAYNYTAITNPTVNSYKRLVPGFEAPVYIAWAGKNRSPLVRVPASRGMGT
                     RLELRSVDPTANPYLAMAVLLEVGLDGIEHKIEAPAPVESNIYAMSDEERREAGITDL
                     PSTLHNALKALRKDDVVRSALGDHIYTNFLEAKKIEWASYATYVSQWEIDNYLDMY"
     gene            complement(2539..4218)
                     /locus_tag="ACHU66_001724"
     CDS             complement(2539..4218)
                     /locus_tag="ACHU66_001724"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFR3389991.1"
                     /translation="MSSVNLKPHEVGVFAIGGLGEIGKNTYGIEYKDEILIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVENLDRVKGLVITHGHEDHIGGIPFLLKQANVPIYAGPLA
                     LALIRGKLEEHGLLRDATLHEINHNTELTFKHLKVSFFRTTHSIPEPLGIVVDTPQGK
                     IVCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIM
                     KLIEGIHGRIIFASFASNIFRLQQAADAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKDTFIEPNEIKEYPASEIMILCTGSQGEPMAALSRIAHGTHRQVQLQPGDTVIFSSS
                     PIPGNTTGVNKLINILIEAGVDVIHGKINNIHTSGHGGQQEQKLMLRLIKPKYFMPVH
                     GEYRMQKIHASLAVDTGVPKDNIFIMENGDVLALTKDSARLAGQFNAQDIYVDGNRIG
                     EIGAAVLKDRRDLSEDGVVLAVATVDFNSKMILAGPDILSRGFIYMRESGELIRESQR
                     VLFNAIRIAMRNKDANIQTVNGAIVNALRPFLYEKTEREPIIIPMILTPDR"
     gene            complement(4222..4452)
                     /locus_tag="ACHU66_001725"
     CDS             complement(4222..4452)
                     /locus_tag="ACHU66_001725"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase subunit epsilon"
                     /protein_id="HFR3389992.1"
                     /translation="MIYKVFYQESKISPRREQTRALYLDIDAASELEGRIIARELVET
                     NRPEYNIEFIDLLSDNHLEYEKEHADFKITEF"
     gene            4899..5582
                     /gene="tsaB"
                     /locus_tag="ACHU66_001726"
     CDS             4899..5582
                     /gene="tsaB"
                     /locus_tag="ACHU66_001726"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR3389993.1"
                     /translation="MKILALDSSNQALSVALVEDGRLQAETLLAVKKNHSISLMPVVD
                     FLVAQVGWTPKDLDRIVVAEGPGSYTGLRVAVATAKTLAYTLKIDLVGLSSLQSLVPP
                     SLTGLVVPLIDARRNCVYAGLYENGRAVEADKYWSFEDLLSSLSGKEDITFVGEVANF
                     IEQIEQALPTAQYQASLPSAYQLAVIGQDLPAVDVTSFEPNYLKRVEAEENWLKDNQA
                     GSESYIKRV"
     gene            5579..6019
                     /gene="rimI"
                     /locus_tag="ACHU66_001727"
     CDS             5579..6019
                     /gene="rimI"
                     /locus_tag="ACHU66_001727"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR3389994.1"
                     /translation="MIEIKYYDGQENLSEAVLAVMESVYEQSPWTLEQIVTSLASQDE
                     DYYLAYEGQELVGFLAVQTVLDEMEILQIAVKADFQRLGIASQLMAAVMDWEGDIFLE
                     VRESNSAAQALYTRQHFTKIGKRKDYYRHPVEDAVLMKRERDER"
     gene            6009..7016
                     /gene="tsaD"
                     /locus_tag="ACHU66_001728"
     CDS             6009..7016
                     /gene="tsaD"
                     /locus_tag="ACHU66_001728"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR3389995.1"
                     /translation="MKDRLILAIETSCDETSVAVLRNDAELLSNVIASQIASHQRFGG
                     VVPEVASRHHVEVITACIEEALLEAEVTAEDLTAVAVTYGPGLVGALLVGISAAKAFA
                     WANGLPLIPVNHMAGHLMAARAVKELEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLPYPAGRVIDELAHEGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAQQKGETLSNADLSASFQACVMDILMAKTKKALEQYPVKTLVVAGGVAANQ
                     GLRERLAAEITDVEVIIPPLRLCGDNAGMIALAAVSEYNKENLAGWDLNAKPSLAFEN
                     L"
     gene            complement(7053..7889)
                     /locus_tag="ACHU66_001729"
     CDS             complement(7053..7889)
                     /locus_tag="ACHU66_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR3389996.1"
                     /translation="MKIQPEFYVFNNRSYVDFYPIQFGMEACKPLHSFGPTMKQHYLF
                     HYIISGSGTFYDTSDQREYHLTAGQGFLISPDAICSYEADEKDPWTYIWLEFDGLKTE
                     HFLRQAGLSKEQPIFSQREMPTSSPVYHEMKQILALHHQKSALVLGHLYLFISCLIDC
                     SLTKTSSKHDENKEFYIREAINFVERNYEKAISVDDLAQVCNLNRHYFSRLFKEQMNI
                     SPQQFIIQYRLSEACELLKNTTKTLQEIAEEIGYSNQFNFSTAFKRHYQISPNRWRKI
                     HK"
BASE COUNT         2064 a         1489 c         2027 g         2317 t
ORIGIN      
        1 gatacccaga agtttaaatt gggtatgaga actttaaaat caggtctagc tgtattttta
       61 gtcattttat tgtttcatct tttaaaatgg gatggtattc agattgcggc tctctcagca
      121 gttttcagtc ttcgtgaaga ttttgggaaa agtgtgcatt ttggctcttc ccgtgtactt
      181 agtaattcca taggtggctt ttatgccttg ctttatttta ttctaagtga tctattttct
      241 cataattttt gggtgacctt aattttcatc cctattttta cgatgctaac tattatgacg
      301 aatgtttcgt ttaataatgc tccaggtgtt attagcgggg ttgctacaat gttgatcatt
      361 gctctctcag ttcctgcagg agattctatt ttctacgccc tccaaagagt ttttgaaaca
      421 ttcgttggag tttttgtatc tgttattgta aattacgata tcgataaaat aaaaaagcgc
      481 tttctttcct aacagcgtca taaaatgtga cacagagggc ttgactgttc gttttttctg
      541 ccctataata gtgtgcagaa aggagttcat tatgagagaa aaagagtttc gacgttcctt
      601 agctattttt ccaattggta gtgtaatgaa attgactgat ttaacagcgc gtcagattcg
      661 ttactatgaa gatcacggtt tgataaaacc agttcggaac gaaggtaatc gccgcttgta
      721 ttctctaaat gatatggact tactacttga aattaaagat tttttggatg agggcctaaa
      781 cattgcagcg attaaaaaag aatacgccaa tcgtaaagca aaggaaaagg ttcaagcaga
      841 acagaaaact ttaactgatg aggatgtgcg tcgtatccta catgatgaat ttagcagaca
      901 agggcgattt tcaagtccaa gctcgatctt tcggtcgtcg taataggttt aattaagagg
      961 agaaaaaata tggttgtcac aacagcggat attaaacgtg atattaaaga gaaaaatgtt
     1021 acatttctac gtctcatgtt cactgatatt atgggagtga tgaaaaacgt agagattcct
     1081 gcgacagaag agcaggttga aaaagtttta tcaaataaag ttatgtttga tggttcgtct
     1141 attgaaggtt ttgtacgtat taatgaatct gatatgtacc tttatccaga tttaaacaca
     1201 tggactattt ttccttgggg agatgaaaat ggccgtgtgg caggcttgat ttgtgatatt
     1261 tatacgactg aaggtgaacc gtttgcggga gatcctcgtg gaaacttgaa aaaatctctt
     1321 cgtcggatgg aaaaagaagg attctcatcc tttaaccttg gtccagaacc agaattcttc
     1381 ttgttcaaaa tggatgagca ggggaatcca actcttgaag tgaatgacaa gggtggttac
     1441 tttgacttgg cgcctacaga tcttgcggac aatactcgtc gtgaaattgt aaatgttttg
     1501 actgaaatgg gatttgaagt tgaagcgagc caccacgaag tagcggttgg tcagcatgaa
     1561 atcgacttta aatatgcgga tgttttgaag gcgtgtgata atatccaaat ctttaagttg
     1621 gttgttaaga cgattgcacg aaaacatggc ttgtacgcta catttatggc taaacctaaa
     1681 tttggaattg ctggttcagg tatgcactgt aacatgtcac tctttgataa agacggtaat
     1741 aatgccttct ttgatccaga agatcctcgt ggcatgcaat tgtcagagac agcttatcac
     1801 ttcctcggtg gtttgattaa acatgcttat aactacactg caattaccaa ccctacagta
     1861 aactcataca aacgtttggt acctggtttt gaagctcctg tttatattgc ttgggcaggc
     1921 aaaaaccgtt ctccattggt tcgtgtacct gcatcacgtg gaatgggaac tcgcttagaa
     1981 ttgcgttctg tagatccaac ggctaaccca tatttggcta tggcagttct tttggaagtt
     2041 ggattagatg gtatcgaaca caagattgag gctcctgctc cagtagaatc aaatatttat
     2101 gcaatgtcag atgaagaacg tcgcgaagct ggtattacag atcttccatc aactcttcac
     2161 aatgcgctta aagccttgag aaaagatgat gttgttcgtt cggcattggg tgatcatatc
     2221 tatactaact tcttggaagc taagaaaatc gaatgggcaa gctatgcaac ctacgtttct
     2281 caatgggaaa ttgacaacta tttggatatg tattaaacaa aggaccccga cgggtccttt
     2341 gttcacgagc cgagaaattg gaaagcgtgt aatggtccag tggagtgaaa tagcctggga
     2401 agagactgtt tcagtctgag ctaataaata gcagggcgag gggatggaca tgagcttgaa
     2461 atctgtcttt ttacaagctg ggttatcttg ttaatataaa ataaatccca gaggtctcac
     2521 ctctgggatt ttagtgtgtt atcggtcagg cgtcagaatc attgggataa tgattggttc
     2581 acgctctgtt ttttcgtata ggaatggtcg tagggcgtta acaatggcgc cgttgactgt
     2641 ttggatattg gcatctttat tgcgcatggc gatgcggata gcgttgaaaa ggacgcgttg
     2701 gctttcgcga atgagctcgc cagattctct catatagatg aagccacggc tgaggatgtc
     2761 tggtcctgct aatatcatct tagaattaaa atcgacagtg gctactgcaa ggacaacacc
     2821 atcttctgaa agatcgcgac ggtcttttag aacagctgcg ccgatttcac cgatgcggtt
     2881 tccatcaaca tagatgtctt gggcgttgaa ttgacctgcg agacgtgcag aatcttttgt
     2941 cagagcaagg acatctccgt tttccatgat gaagatatta tcttttggaa cgcctgtatc
     3001 aacagctagg ctggcatgaa ttttttgcat gcggtattca ccatggacgg gcatgaagta
     3061 ttttggcttg attaggcgga gcatgagttt ttgctcttgt tgaccaccgt gtccagaggt
     3121 atggatgttg ttgatcttgc cgtgaataac atcgacaccg gcttcaatta agatattgat
     3181 gagcttgttg acacctgtgg tatttcccgg aataggactg gacgagaaga taaccgtgtc
     3241 gcctggctgg agttgcacct gacggtgggt accgtgggcg atgcgagaga gtgctgccat
     3301 aggctcccct tggctccctg tacagagaat catgatctcg ctggcaggat actctttgat
     3361 ctcgtttggc tcgataaagg tgtcttttgg aaccttgatg tagccgagct cgataccgtt
     3421 aacaatggct ttttccatgg aacgtccaaa gacagcgatc ttgcgtccgg tcttaaccgc
     3481 tgcatcagcc gcctgttgga gacggaagat gttggaggca aaggaagcaa aaatgatgcg
     3541 tccgtgaatg ccttcaatca gcttcataat ggactgaccg accacttttt ccgagttggt
     3601 aaaggttggg acctcagcat ttgtcgagtc ggagaggagg cagagaacgc cttcttcacc
     3661 gagggcagcc atgcggtgaa ggtctgctgg ctcgccaact ggagtgaagt cgaacttgaa
     3721 gtcaccggtg cagacgattt ttccttgcgg ggtgtcaacg acgataccaa gtggttctgg
     3781 aatggagtgg gtggtgcgga agaagctgac cttgaggtgt ttgaaggtca actcagtatt
     3841 atgattgatt tcatgcaaag tcgcatcacg gagcagtccg tgttcttcca atttgcctcg
     3901 aataagggca agggctaaag gtccagcata gattgggaca ttggcctgct tgagtaagaa
     3961 gggaatgcct ccgatgtggt cttcgtgccc gtgggtaata accagacctt taacacggtc
     4021 taggttttca acgatgtagg agtagtctgg gataacgtag tcgataccca ataggtcatc
     4081 ttctgggaac ttgataccgg catcgacgat gagaatttca tccttatact cgataccgta
     4141 ggtatttttc ccgatttctc ccaaaccacc gatggcgaaa acgccaactt catgaggttt
     4201 tagattgact gatgacatgg attaaaactc cgtaattttg aaatctgcgt gctctttctc
     4261 gtattcaaga tgattgtctg ataagaggtc gataaattcg atgttgtatt cagggcggtt
     4321 ggtttccacc aattcacgcg cgataatacg accttccaat tctgatgcag catcgatgtc
     4381 tagataaaga gcacgtgttt gctcgcgacg tgggctaatt tttgattctt gatagaatac
     4441 tttataaatc atgtgtagta tagttccttt ctttttgact ggcaactatg aaaaaagcct
     4501 caaacagttg ggtctgtctg gtaatatcta ttcaattttc tcgagttgtc aatttattca
     4561 gtctaaatgt actcttcatt atatcataaa atatgctgat ggtaaaaggg tttggtagga
     4621 aaatggaaaa gtgtcatgat tttcagaaat tcggtatttc agaaggggat aaatcaaaga
     4681 aatgagttag gattccgagt gtgttgggat tactaagact aagttcaatt gtgctgagaa
     4741 atgcctgtca actgccagat agtgatgtga cttactagaa tgacaaatcg gttcgatcca
     4801 ttaacctact agaaatcaca accgattctt ttcattattt gaaaaaaagg tcaagatagc
     4861 ttacaataga gggtagcatt gtacagaaag agaaatttat gaaaatacta gctttagata
     4921 gctccaatca ggctttatca gttgccttgg tggaggacgg tcgtttgcag gcggaaaccc
     4981 tgctggccgt taagaaaaat catagtatca gcctcatgcc tgtggtcgat tttttggtag
     5041 cacaggtggg atggacgccc aaagacttgg ataggatcgt ggtggctgag ggacctggtt
     5101 cctacacggg tcttcgtgtg gcggtggcga ccgccaagac cttggcctac accctcaaga
     5161 ttgacttggt ggggctatcc agtcttcagt ctctggttcc tcccagcctg acgggtctgg
     5221 tggtgcctct cattgatgcc cgccgtaact gcgtctatgc aggtctttat gaaaatggca
     5281 gagcagttga ggcagacaag tactggtctt ttgaagactt actttctagc ctgtctggga
     5341 aggaagacat cacctttgtc ggagaagtag caaactttat cgagcaaatc gagcaagctc
     5401 tgccgactgc tcagtatcaa gccagtctac cgtctgccta tcaattggca gtaatcggtc
     5461 aagacttgcc agcagtagat gtgacgagct ttgagcccaa ctatctcaag cgggtagagg
     5521 cagaggaaaa ctggctcaag gacaaccaag ctggctcaga aagttatatc aagcgtgtat
     5581 gatagagatt aagtactacg acggtcagga aaatctatcg gaagctgtgt tggcagtcat
     5641 ggagtcggtc tatgagcaat cgccttggac cttggaacag attgtgacta gcctagccag
     5701 tcaggatgag gattattatc tggcctatga gggtcaggaa ttagttggat ttttggctgt
     5761 tcagacggtg ctggatgaga tggaaatctt gcagattgct gtcaaggctg attttcagag
     5821 gttgggaatc gccagtcaac tgatggctgc tgtgatggac tgggaggggg acatcttcct
     5881 cgaagtgagg gagtccaata gtgcagctca ggccctctat acacgccagc attttaccaa
     5941 aataggaaaa cgaaaagact actaccgcca tcctgtcgag gacgcggtgc taatgaagag
     6001 agaacgtgat gaaagataga ttgattttgg cgatcgagac ctcctgtgac gagacctcgg
     6061 tggctgtttt gcgaaatgat gcggagcttt tgtccaatgt cattgctagc cagattgcca
     6121 gccaccagcg gtttggcggg gtggtgcctg aagtggccag ccgtcaccat gtggaagtga
     6181 ttacggcttg tatcgaggag gccttgttgg aggcagaggt gacggcagag gacctgacgg
     6241 ctgtggctgt gacctatggg cctggcttgg tcggtgcctt gctagttggg atttcggctg
     6301 ccaaggcatt tgcttgggct aacggtctgc cactcattcc tgtcaatcat atggcagggc
     6361 atttgatggc ggcgcgtgcg gtcaaggagt tggagtttcc gctcttggcc ctcttggtca
     6421 gcggtggaca cacagaattg gtctatgtgt cagaggcggg cgactataag attgtcggtg
     6481 aaacgcgaga tgatgcggtc ggcgaagcct atgataaggt ggggcgggtc atgggcctgc
     6541 cctatccagc tggtcgggtg attgacgaat tggcacatga ggggcaagac atttatgact
     6601 tccctcgggc tatgattaaa gaggataatc tggagttttc tttctctggt ttgaaatccg
     6661 cctttatcaa cctctaccac aatgcccagc agaaagggga gaccttgtcc aatgcggact
     6721 tgtcagcctc tttccaagcc tgtgttatgg acattctcat ggctaagacc aagaaggcct
     6781 tggagcaata ccctgtcaaa accttggtgg tagcgggcgg tgtggcagct aatcagggct
     6841 tgcgagagcg gttggctgct gaaattaccg atgtggaggt tatcattccc ccgcttcgcc
     6901 tctgcggtga caatgcaggt atgattgcct tagcagcggt cagtgagtac aataaggaaa
     6961 accttgctgg ctgggacctc aatgccaagc ctagtctggc ttttgagaat ttatagaata
     7021 aaaacctctt acgtcaagga tacgtaagag gtttatttgt ggatttttcg ccagcgattt
     7081 ggagaaattt ggtaatgtct tttaaaagca gttgaaaaat tgaattggtt ggagtagccg
     7141 atttcttcag caatttcctg tagtgtctta gtggtgtttt ttaagagttc gcaggcttcg
     7201 ctgagtcggt attggatgat aaattgttga ggtgagatgt tcatttgctc cttgaatagc
     7261 cgactgaagt agtggcgatt gaggttgcat acttgtgcca aatcatcgac ggatatagct
     7321 ttctcatagt ttcgttctac gaagttgata gcttctcgta tataaaattc cttgttttca
     7381 tcgtgtttgc tacttgtttt ggttagcgaa cagtcaatga ggcaggagat aaagaggtaa
     7441 aggtgtccga gaactagagc gcttttttga tgatggagcg cgagaatttg tttcatttca
     7501 tggtagacag ggctagaggt gggcatttct cgttgtgaaa agataggttg ttctttcgag
     7561 agccctgcct gtctgagaaa atgctcagtt ttcagaccgt caaattcaag ccagatatag
     7621 gtccaggggt ccttttcgtc tgcttcgtag ctacagatgg cgtctgggga gataaggaag
     7681 ccctgtcctg cggttaggtg gtattcacgt tggtcggaag tatcgtaaaa agtacctgaa
     7741 ccagagatga tgtagtgaaa caggtagtgt tgtttcatag ttgggccgaa tgagtgaagt
     7801 ggcttacagg cttccatgcc aaattgaatg gggtaaaaat ctacatagga tctgttatta
     7861 aagacgtaaa attcaggttg tattttcatc gtattct
//
LOCUS       DAZQCJ010000091         7739 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000091 DAZQCJ010000000
VERSION     DAZQCJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7739
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.092"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(58..1278)
                     /gene="pepT"
                     /locus_tag="ACHU66_001730"
     CDS             complement(58..1278)
                     /gene="pepT"
                     /locus_tag="ACHU66_001730"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR3389997.1"
                     /translation="MKYPTLLDRFLVYVKENTRSDENSTTTPSTQNQVEFAQNILLPE
                     MKRIGLQNVHYLPNGFAVGTLPANDPSLTRKIGFIAHMDTADFNAEGVNPQIIENYDG
                     NPIALGTSGYELHPKDFPQLANYHGQTLITTDGTTLLGSDDKSGIAEIMTAIEFLVQN
                     PDIKHCEIRVGFGPDEEIGVGADKFDVEDFYVDFAYTMDGGPLGELQYETFSAAGAKI
                     DFLGRNVHPGSAKDQMINAFQMAIDFHNALPEADRPEKTEGYEGFFHLMNMEGSVDTA
                     STTYIIRDFEEEDFLARKQLMLDIAEKMNAKFDTPRVIVNLHDQYYNMKKIIEKDMTP
                     INIAKDVMENLGIKPLIEPVRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLETM
                     EKAVDVILGIVAYK"
     gene            complement(1288..2103)
                     /locus_tag="ACHU66_001731"
     CDS             complement(1288..2103)
                     /locus_tag="ACHU66_001731"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFR3389998.1"
                     /translation="MIKLIALDMDGTLLNEKKELMQPQIDAIHQAVEAGVKIVLCTGR
                     PLAGVKPFVEQLGFDTEEEFIIVNNGCSTHSTKDWSLIDWEELSISDIDYLSTFIEND
                     DVQISLFDEEDYFVLAEKANARVNLDAGLVGMTPQPIVLKEAQSGQHRFFEAMFVGEK
                     EHIDAFENQHNPVLSQHYSTVRSQDYLLEILPNGASKASGLKKLADRLGILPEEIMAM
                     GDANNDLEMIEFAGLGIAMGNANEQVKAIAQAITDTNENNGVAKAIEKHILNK"
     gene            2485..2955
                     /locus_tag="ACHU66_001732"
     CDS             2485..2955
                     /locus_tag="ACHU66_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR3389999.1"
                     /translation="MKISVKKEAGHYLLSICYSVSASLTEAWDLLATDSGFAHWFPQL
                     NIVGNTLVFEMEGFREVMDLLEYRKNEKIAYRWDSATVSFTLSQLENQTLITFEERIP
                     EDFGNEFANAQKDMTGWLVPNECIKKFLEGQEPPVCQPLQEKWRTFLELELEGL"
     gene            2973..3230
                     /locus_tag="ACHU66_001733"
     CDS             2973..3230
                     /locus_tag="ACHU66_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390000.1"
                     /translation="MKKWWEQLKEEYYETRFDSNEDWMEWFIKRKMTWGWRWPTAITI
                     FVLWALSLPDARNVVRFFYFLLTLGVATVLHKLISYLKRRK"
     gene            3237..4490
                     /locus_tag="ACHU66_001734"
     CDS             3237..4490
                     /locus_tag="ACHU66_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HFR3390001.1"
                     /translation="MHQITCPHCGTAFQVNETEYSQLLAQVRGAEFDKEIHDRLERER
                     ELLSQKAENDLQARLSDKDKEILELTAKLDKFASQTELEISSAISQKDQEIQELKAQL
                     GQIGLAKDLELQQAVAQVEKERDAAQNALVLQEQKQELALATTRQEYEVRLKAADEQV
                     EFYKNFKAQQSTKAIGESLEQFAEAEFNKVRSYAFPHAKFAKDNEVSASGSKGDFIFR
                     DFDESGLEFISIMFEMKNEADTTKTKHKNADFFKELDKDRREKKCEYAVLVSMLEADN
                     DYYNTGIVDVSHEYEKMYVIRPQFFIQLIGILRNAALNSLQYQQELALVKEQNIDITH
                     FEEDLEIFKNAFAKNYQSASNNFQKAIDEIDKAIKRMEAVKAALTTSENQLRLANNKL
                     DDVSVKKLTRNNPTMKAKFEALREE"
     gene            4492..4986
                     /locus_tag="ACHU66_001735"
     CDS             4492..4986
                     /locus_tag="ACHU66_001735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR3390002.1"
                     /translation="MIVPLQFEHILQLDEAFQKQGWPSSKEILTNYLREQEKGERIVL
                     VAEETGVCKGYITLIKQVKEGPFYQSGIPEIADFNVFEDFQNQGIGWQLLDAICQLAS
                     EFTDIVGIGVGLNANYGKAQRLYVKHGFIPDGSGVWYRGRSLPVGAKAYNDDELALYF
                     TKKL"
     gene            4999..5229
                     /locus_tag="ACHU66_001736"
     CDS             4999..5229
                     /locus_tag="ACHU66_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HFR3390003.1"
                     /translation="MNTIDLNLPVAEIINQHPEVKDILVELGFKPLANPAMLNTVGKV
                     TNLKTGSKMTKIPLDRIQQVLECNGYEVIGGE"
     gene            5229..6554
                     /locus_tag="ACHU66_001737"
     CDS             5229..6554
                     /locus_tag="ACHU66_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HFR3390004.1"
                     /translation="MTEQRIDILKNILLDLHNGASPESVQDQFNQHFTGVSALEISMM
                     EHELMSSDTGITFEDVMNLCNIHANLFKGAIADVEVADMDQEGHPVYVFKQENLALRA
                     ALLRIRRIIDQHEQTEDAELKDQLLQGLTRQFGLLGQFENHYTRKEKVFFPIMERYGH
                     DAPPKVMWGVDDEIRDLFKTARKALEGGDLAATRAHFENFAKEFEEMIFKEEAILLMI
                     LLEIFTQDDWLQVAADSDAYGYAIVKPTAKWVPHRELFGEIAEQEVDSISPQPASPNQ
                     QIIDTPEGQFTITFTPKEKSETVQDRTSPQTFGNGYLSVEQANLILNHLPLELTFVNK
                     DDIFQYYNDSHPVEDMIFKRTPSQIGRHVELCHPPKIVDKVKKIFELLRTGQKDQVTM
                     WFKSESMGKFVYVVYKAVRDDQGEFQGVLEYVQDIQPFFEIDSDFHREI"
     gene            6585..6794
                     /locus_tag="ACHU66_001738"
     CDS             6585..6794
                     /locus_tag="ACHU66_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390005.1"
                     /translation="MDWPTIKIGDEGYYDAPLIFSYGYDEEAEEGIDEAVTFDAVPSE
                     TMRRIHKELENLIDTTFGSYRFYDD"
     gene            6844..7722
                     /gene="truB"
                     /locus_tag="ACHU66_001739"
     CDS             6844..7722
                     /gene="truB"
                     /locus_tag="ACHU66_001739"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR3390006.1"
                     /translation="MISGIINVKKEAGMTSHDVVFKLRKILQEKKIGHGGTLDPDVTG
                     VLPIAVGKATRMIEYMQEEGKIYEGEITLGFSTTTEDSSGDLVERTPVLDIAEQAVDE
                     AMTSFVGTITQIPPMYSAVKVNGRKLYEYARTGEEVERPQRQIEIYSFERTSPIELVD
                     DCARFTFRVRCGKGTYVRTLSVDLGAKLGYASHMSKLERTGSAGMDLADALTLEEISS
                     LVAENDFSFLQPIERGIGDLPVVELKEEQVQDATFGRFVELNAEAERLAGFHDGQLIA
                     IFEKRDQLYKPSKVLV"
BASE COUNT         2239 a         1497 c         1897 g         2106 t
ORIGIN      
        1 atttttttaa aattttttca gaaaaaaacg actttctatt gaaagccgtt tgaaatttta
       61 tttgtaagcg acgattccaa ggatgacatc aacagctttt tccatggttt ccaagctgac
      121 gaactcgaag cgaccgtgca tgttttcgcc acctgcgaag atatttggtg ttggaatgcc
      181 catgaaggaa atcttagagc cgtctgttcc tccgcgaaca ggctcaatca gtggcttgat
      241 gcctagattt tccatgacgt ctttggcaat gttgattgga gtcatatctt tttcgatgat
      301 ttttttcatg ttgtagtatt ggtcgtgcag gttgacaatg acgcggggtg tgtcaaactt
      361 ggcattcatt ttctctgcaa tatccagcat gagctgtttg cgggcaagga aatcttcttc
      421 ctcgaagtca cggatgatgt aggtggtaga agcagtatca acactacctt ccatattcat
      481 caaatggaag aaaccttcat agccttctgt tttctcaggg cggtctgctt ctggaagggc
      541 attgtggaaa tcaatagcca tttggaaggc attgatcatc tggtccttag cagatcctgg
      601 gtggacattg cgtcctagga aatcaatctt ggcaccagct gctgagaagg tttcgtactg
      661 gagttcgccc agcggtccac cgtccatggt gtaggcaaag tccacataga agtcttccac
      721 atcaaacttg tctgctccga caccgatttc ttcgtcagga ccaaagccga cacggatttc
      781 acagtgtttg atgtcaggat tttggactag gaattcaatc gcagtcatga tttctgcaat
      841 tcctgacttg tcatcagagc ctagcaaggt agtaccgtca gtggtaatca aggtttgacc
      901 gtggtagttg gcaagttgtg ggaagtcttt tggatgcaat tcatagccag aagttcccaa
      961 ggcaattggg ttaccgtcgt agttttcgat gatctgcgga ttgacacctt cagcgttgaa
     1021 atcagccgta tccatgtggg cgatgaagcc aatcttacgt gtcaaacttg ggtcgttggc
     1081 aggcagtgtt cccacagcaa agccgtttgg taggtagtgg acattctgta agccgatgcg
     1141 tttcatttca ggcagaagga tattctgtgc aaattcgact tggttttggg tagaaggtgt
     1201 ggtggtcgaa ttttcatctg agcgagtatt ttctttgaca tagactaaaa aacggtctaa
     1261 gagagttggg tatttcatag tatctcctta tttgttcaaa atgtgttttt caatggcttt
     1321 ggcaacgccg ttgttttcat tggtgtctgt gatggcctgg gcgatagcct tgacctgctc
     1381 gttggcgttg cccatggcaa taccaagtcc agcaaattca atcatttcca ggtcgttgtt
     1441 ggcatctccc atagccatga tttcttctgg gaggataccg aggcggtctg ccagtttttt
     1501 caaaccagag gccttgcttg caccgtttgg taaaatttcc aagagatagt cctgtgaacg
     1561 aacggtggaa tagtgttggc tgagtacagg attgtgttga ttttcaaagg catcaatatg
     1621 ctctttttca ccgacgaaca tggcttcaaa gaagcgatgc tgacctgact gggcttcttt
     1681 taaaacaatt ggttgaggag tcattcctac taaacctgcg tctaaattaa cgcgtgcatt
     1741 ggctttttct gcaagaacga agtagtcttc ctcatcaaaa agagagattt gcacatcatc
     1801 attttcaata aaagtggaaa gataatcaat atcagagata gatagttctt cccagtcaat
     1861 caggctccaa tcctttgtcg aatgcgttga acaaccgtta ttgacaatga taaattcttc
     1921 ctcagtgtca aagccgagct gctctacgaa aggtttaaca ccagccaagg gccgaccagt
     1981 acaaagaaca atcttgacgc ccgcttcaac agcctgatga atggcatcaa tctgtggctg
     2041 catcagctct tttttctcgt ttaaaagggt tccgtccata tcaagagcga ttagtttaat
     2101 catataaacc tccttgtttt caatacatct agtatagcac aaaatgagaa aggaatgatt
     2161 gaaatactaa actctgttct atatgttaaa ataaattagg tgacgaatga aatatagaag
     2221 tgttgcaaaa atgacaatgt ccattatgtg ttaagaaagt ctaacattgc taggggatac
     2281 aaaacatgga gtatgagcca ggtaaacaaa ggtaaggact gtctatgaaa gatgttataa
     2341 aaaataattc agatttcaag ataccgtttt tcgatcctct ggaatgaata gcattcatgc
     2401 ttaaagaata gagacaatgg aaatatcggt tatagaccgg tgttttttgg tataattaat
     2461 agaaggatag aataggagca tgatatgaag attagtgtga agaaagaggc ggggcactac
     2521 ctgctcagca tttgctattc tgtgtcggct agtttgacag aagcttggga cttgttggcg
     2581 acagacagtg gttttgccca ctggtttcca cagttgaaca ttgtaggaaa tacgctggtt
     2641 tttgaaatgg aaggattccg tgaagttatg gatttactgg aatatcgaaa aaatgagaaa
     2701 attgcctaca ggtgggattc agcaacagtt tcatttacac tgtctcagct agaaaatcag
     2761 accttaatta cttttgaaga acgaattcca gaagattttg gtaatgaatt tgcgaatgct
     2821 caaaaagaca tgacaggatg gttggtgcca aatgaatgta tcaaaaaatt cttagaaggt
     2881 caggagccgc ctgtgtgtca acccttgcag gaaaagtgga ggacttttct ggagcttgaa
     2941 ttagaaggtc tgtgatctga aaggaggctg ttatgaaaaa gtggtgggaa caactgaaag
     3001 aagaatatta tgaaacacgg tttgattcta atgaagattg gatggaatgg tttatcaaac
     3061 ggaagatgac ctggggctgg agatggccga ctgctataac gatttttgtc ctttgggctc
     3121 tatcacttcc agatgctaga aatgtggttc gtttctttta ttttttattg acgctgggag
     3181 tagctacggt tttacataaa ttaatatctt atttgaaaag aagaaagtga gagtatatgc
     3241 atcaaattac ttgcccccat tgtgggacgg cctttcaggt caatgaaaca gaatatagtc
     3301 agcttttagc ccaggtgcgt ggtgcggagt ttgacaagga aatccacgac cgtttggagc
     3361 gggaacggga attattaagc cagaaggctg aaaatgattt gcaggctaga ttgagtgata
     3421 aggataagga gattctggaa ctgaccgcaa aacttgataa atttgctagt cagacggaac
     3481 tggaaatcag ctctgctatt tcccaaaaag accaagaaat ccaagagctt aaggctcagt
     3541 tggggcaaat cggtcttgcc aaggacttgg agttgcagca ggcggtagct caggtggaaa
     3601 aagagcggga tgcggcacaa aatgctttgg ttttgcagga acaaaagcaa gagttagccc
     3661 tagcgaccac tcgtcaggag tacgaagtac ggctcaaggc ggcggatgag caggtggaat
     3721 tttacaaaaa tttcaaggcc cagcagtcta ctaaggcaat tggagagagc ttggagcaat
     3781 ttgctgaggc ggagtttaac aaggttcgtt cctatgcttt tccacatgcc aaatttgcga
     3841 aggacaacga agtatcagcg tccggttcaa aaggagactt catcttccgt gattttgacg
     3901 agtcgggctt ggaatttatt tccatcatgt tcgaaatgaa aaacgaggca gatacgacca
     3961 agaccaagca taaaaacgct gatttcttta aggagttgga taaggaccgt cgggagaaaa
     4021 aatgcgaata tgctgtatta gtcagcatgc tagaggcgga caatgactat tacaatacag
     4081 ggattgtgga tgttagtcat gaatatgaga agatgtatgt tattcgacca cagttcttta
     4141 tccaattaat cggaatttta cgaaatgctg cgctcaatag tctgcaatat cagcaggagt
     4201 tagccttggt taaggagcag aatattgata ttactcattt tgaagaagat ttggaaattt
     4261 tcaagaatgc ttttgccaaa aactatcaat cggccagcaa caatttccaa aaagctattg
     4321 atgaaatcga taaggcaatc aagcgaatgg aggctgtcaa ggcagccctg accaccagcg
     4381 aaaaccagct tcgcctagcc aataataaac tagacgatgt ctccgtcaaa aaactaaccc
     4441 gcaataaccc aaccatgaaa gccaagtttg aggcgttgag ggaggagtag gatgattgtt
     4501 ccgttacaat ttgagcatat acttcaactt gatgaggcct tccagaagca aggttggccc
     4561 tcaagcaaag agattttaac caactattta agggaacaag aaaagggaga acgtatcgtc
     4621 ctagttgctg aagaaacggg agtttgtaaa ggttatatca ccttgattaa gcaagtgaag
     4681 gaggggccgt tttaccaatc tggaatacct gaaattgctg atttcaatgt ttttgaagac
     4741 tttcaaaatc aggggatagg ttggcaatta ttagatgcta tctgtcagct tgcctcagaa
     4801 ttcacggata tagtaggtat tggagttggt ttgaatgcaa attatggaaa ggctcaacgg
     4861 ctctatgtaa aacatggttt cataccagac ggaagcggtg tctggtaccg agggcgctct
     4921 ttgccagtag gtgccaaagc ctataacgat gatgaattgg ccttgtattt tacaaaaaaa
     4981 ttatagagga aaagtaaaat gaacaccatc gacctaaatc tgcctgttgc agagattatc
     5041 aaccagcacc cagaagtcaa ggatattctg gtggagcttg gtttcaagcc tttggcaaat
     5101 ccagctatgc tcaacacagt tggtaaggtg accaatctga agacaggctc caagatgacc
     5161 aagattccgt tagaccgtat tcaacaggtc ttggaatgca acggctatga ggtaatagga
     5221 ggagaataat gacggaacaa cggattgaca ttttgaagaa tattctcttg gacctgcaca
     5281 atggtgccag tccagagtct gttcaggacc agtttaatca gcatttcacg ggagtttctg
     5341 ccttggaaat ctccatgatg gagcatgaac tcatgtccag cgacacgggt atcacttttg
     5401 aagacgtcat gaacctttgc aatatccacg ccaacctttt caaaggagcc attgcggatg
     5461 tggaagtggc ggatatggat caggaaggac accccgtcta tgtcttcaag caggaaaatc
     5521 tagcactgcg ggcggctctt ttacgcatcc gacgtatcat tgaccagcat gagcagacag
     5581 aggatgcgga gctgaaagac cagctcctgc aaggtttgac acgccagttt ggcctcctcg
     5641 gtcaatttga aaatcactat acccgcaagg aaaaggtctt ctttccgatt atggagcggt
     5701 acggtcatga tgcaccgccc aaggttatgt ggggtgtgga cgacgagatt cgcgatctct
     5761 tcaagacggc ccgcaaggca cttgaaggag gtgacctagc tgcgacacgt gctcactttg
     5821 aaaacttcgc caaggaattt gaagagatga ttttcaagga ggaggccatt ctcctcatga
     5881 ttctgctgga gattttcacc caggacgact ggctccaagt ggcagcagac agcgatgcct
     5941 acggctacgc cattgttaag ccgacagcca agtgggtacc tcatcgggag ctttttggag
     6001 aaatagcaga gcaggaagta gatagcattt ctccgcagcc agctagccca aaccagcaga
     6061 ttatcgacac gccagaaggc cagtttacca ttacctttac acccaaggaa aagtcagaaa
     6121 ctgttcagga caggactagt ccgcagacct tcggcaatgg ttatttatct gttgagcaag
     6181 ccaatcttat cctcaatcat ttaccgctgg aattaacctt tgtcaacaag gacgacattt
     6241 tccagtacta caatgacagc catcctgttg aagacatgat tttcaaacga acaccaagcc
     6301 agattggacg ccatgtggag ctctgccatc ctcccaaaat agtggacaag gtcaagaaga
     6361 tttttgaatt gctccgcaca gggcaaaaag accaggtcac catgtggttc aagtccgaga
     6421 gcatgggcaa gtttgtctat gtggtctata aggcagtgcg tgatgaccaa ggagagtttc
     6481 aaggcgtttt ggagtatgtt caggatattc aaccattttt tgagattgat agcgattttc
     6541 atcgggaaat ttagtttagt catttttgaa aggaaattaa gctgttggat tggccgacca
     6601 taaaaattgg tgacgaaggc tattatgatg ccccattgat attttcttat ggctatgatg
     6661 aagaggctga ggaagggatt gatgaagcag taacattcga tgcagtacct tctgaaacca
     6721 tgcgacgcat tcataaggaa ctagagaatt tgatagatac aacattcggt agctatcgtt
     6781 tctatgatga ctgattgata tcggaccgcc cacaagagcg gtcctttgtg ttataatgga
     6841 aaaatgattt caggaattat taacgtaaaa aaagaggcag gtatgacctc gcatgatgtt
     6901 gtttttaagc tccgcaagat tttgcaggag aagaagattg gacatggtgg gaccttggat
     6961 cccgatgtga caggcgttct gcccatcgca gtcggtaagg ccacgcgtat gattgagtac
     7021 atgcaggaag agggaaagat ttacgagggg gagattaccc tcggcttctc aaccacgacc
     7081 gaagattcgt cgggcgacct cgtcgagcgg acaccggttt tggacatagc agagcaggca
     7141 gttgatgagg ctatgaccag tttcgtcggt actattaccc agattccgcc tatgtactct
     7201 gctgtcaaag taaacggtcg caaactatac gaatacgcac ggacgggtga agaagttgag
     7261 cgtcctcaac gccaaattga gatttacagt tttgaacgga ctagcccgat tgaactagta
     7321 gatgactgtg cacgttttac cttccgagtt cgctgtggta agggcaccta tgtccgtacc
     7381 ctgtctgttg acctaggggc taagttgggc tatgccagcc atatgtccaa gttggaacga
     7441 acaggatcag ctggaatgga cctagcggat gccttgactt tagaagaaat ttcttcgctg
     7501 gtagctgaaa atgatttttc attcctccaa cctattgaac gtggcatcgg ggatttacct
     7561 gttgtagaat tgaaagaaga gcaggtgcaa gatgctacat ttggtcgatt tgtagaattg
     7621 aatgcagaag cagagcgctt agcaggcttt catgatggtc agttgattgc tatttttgaa
     7681 aaacgcgacc agctgtataa accgagcaag gttttggtct aagaaatttc tcaataact
//
LOCUS       DAZQCJ010000092         7463 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000092 DAZQCJ010000000
VERSION     DAZQCJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7463
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.093"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..1316)
                     /locus_tag="ACHU66_001740"
     CDS             complement(<1..1316)
                     /locus_tag="ACHU66_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GBS Bsp-like repeat-containing protein"
                     /protein_id="HFR3390007.1"
                     /translation="MFVKKRTLLFLSLCASALNAQLVQADMVNAPSSQVSSSQVAETG
                     GQLSVVDEAGQIKAILSNIQGEIIGVTAQFSTETNSGITVTFSMDEQGRYVAVLDRSA
                     FAEEDQVFSLKLTAQLTDGSFQTLSDYSFEWKKDVVSAAEKPTALDNSDATPSKIEEE
                     ATSTTTSSTVNSSLTAGATSSTSNSLTRSSSNTIAGSSNGSASVPSARVSTASSSVNV
                     TQPTGTITIENRNDAQGTFDVRVTNVSSPKDIASVILPTWSQSDDLRWYEATRQSDGS
                     YKLTVNKKDHKYRTGTYTVHLYYKDSSGGLTGAGGTTTHLSEVKPTGTITIENRNDAQ
                     GTFDVRVTNVSSPKDISSVILPTWSQTDDLRWYEATRQSDGSYKLTVNKKDHKYRTGT
                     YTVHLYYKDSNGGLTGAGGTTTHLSEAKPTGTITIENRNDAQGTFDV"
     gene            complement(1384..2424)
                     /locus_tag="ACHU66_001741"
     CDS             complement(1384..2424)
                     /locus_tag="ACHU66_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFR3390008.1"
                     /translation="MKKKAIVKPLLMSGIFLALLGSATLPSGAVPVVAAETSQSTTYH
                     LTDDEKVAVREYIQAKMTIDMQEYRLAFLEGMMEEMASGSAEAAWDEEIADLKANLTA
                     EQVVVLDELEANLIGSIAQHYHYLFETLTVAGKSGREEAAAIVSKYESEDDASTPEAE
                     LAALKYAREVIVELLNKESAAIDNYIAYAEATGQELAGLLESGNSNLESITSATIGYG
                     QALATASQPKFPYDFSEMDRQIAELTASLQSKVEDKSTAKTENTGVQTSQSATNGSNE
                     QDVPGQGGGQISDVTTGKGNISEAGQKKVIPNDNAKVLPKTSGKSSLPLTVLGLITIF
                     AGWLLTKKQEEK"
     gene            complement(2441..3292)
                     /gene="rfbD"
                     /locus_tag="ACHU66_001742"
     CDS             complement(2441..3292)
                     /gene="rfbD"
                     /locus_tag="ACHU66_001742"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019780653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFR3390009.1"
                     /translation="MILITGANGQLGTELRYLLDERGVDYVAADVAEMDITDADKVDA
                     FFAEVKPSVVYHCAAYTAVDMAEDEGKELNYKINVTGSENVAKAAAKYCATLVYISTD
                     YVFNGDLPVGKEWQVDDQPDPQSEYGRTKRKGEEAVEKFADKFYTVRTAWVFGNYGKN
                     FVFTMQNLAETRDTLTVVNDQHGRPTWTRTLAEFMVHLVDTKQAFGYYHLSNDAAEDT
                     TWFDFATEILKDTNTKVLPVDSSQFPAKAKRPFNSTMSLDKAKATGFVIPTWQEALEA
                     FYKQEKK"
     gene            complement(3347..4393)
                     /gene="rfbB"
                     /locus_tag="ACHU66_001743"
     CDS             complement(3347..4393)
                     /gene="rfbB"
                     /locus_tag="ACHU66_001743"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR3390010.1"
                     /translation="MSQFKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGN
                     KANLEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETNYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLY
                     GEGKNVRDWIHTNDHSTGVWAILTKGRMGETYLIGADGEKNNKEVLELILEKMGQPKD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWTPQFTDFSQGLEETIQWYTDNQDWWKAEKE
                     AVEANYAKTQEVIK"
     gene            complement(4581..5066)
                     /locus_tag="ACHU66_001744"
     CDS             complement(4581..5066)
                     /locus_tag="ACHU66_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFR3390011.1"
                     /translation="MFQAFLEIAEQLNKLGITPLLMGSVGLERRTGRDWQARDLDIHV
                     PSDPRGWEAPDEERIYRADELIVLMNQLGYVLVDRHEHEFHKDGLSVEFGGLHSLPEF
                     AGVELEDLEELETVGVRYYLPTLEQYLKIYQASSKDSYRAENNNQKDFAKIAYLKEIL
                     R"
     gene            complement(5066..5659)
                     /locus_tag="ACHU66_001745"
     CDS             complement(5066..5659)
                     /locus_tag="ACHU66_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HFR3390012.1"
                     /translation="MTENFFGKTLAARPVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGTWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDFVAYSYLVNDYWALELKPKYAFVNYA
                     DPSLDIQWENLEEAEVSEADKNHPLLKDVKPLRKEDL"
     gene            complement(5659..6528)
                     /gene="rfbA"
                     /locus_tag="ACHU66_001746"
     CDS             complement(5659..6528)
                     /gene="rfbA"
                     /locus_tag="ACHU66_001746"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR3390013.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFKDMLGDGSELGISLSYAEQPSPDGLAQAFIIGEEFIGDDNV
                     ALILGDNIYHGNGLTKMLQRAASKEKGATVFGYQVKDPERFGVVEFDADMNAISIEEK
                     PEEPKSNFAVTGLYFYDNDVVEIAKNIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRLQNVQVANLEEIAYRMGYITKEQVYELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            complement(6626..7449)
                     /locus_tag="ACHU66_001747"
                     /pseudo
     CDS             complement(6626..7449)
                     /locus_tag="ACHU66_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659387.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
BASE COUNT         2057 a         1733 c         1444 g         2229 t
ORIGIN      
        1 acatcaaagg taccttgggc atcattacga ttttcaattg tgattgtacc tgttggcttc
       61 gcttctgata aatgagttgt tgttccacca gcacctgtca aaccaccatt tgaatcctta
      121 taatataagt gaaccgtgta ggtacctgta cgatacttat ggtccttctt attaacagtt
      181 aacttatagc tgccatctga ctgacgagtg gcttcatacc aacgtaaatc atccgtctgt
      241 gaccaagtgg gtaaaataac acttgatata tctttcggtg atgaaacatt agtaacacga
      301 acatcgaagg tgccttgggc atcgttacga ttttcaattg tgatggtacc tgttggcttc
      361 acttctgata aatgagtcgt tgtgccacct gcacctgtta ggccaccact tgagtcctta
      421 taatataagt gaactgtgta ggtacctgta cgatacttat ggtccttctt attaacagtt
      481 aacttataac tgccatctga ctgacgagtg gcttcatacc aacgtaaatc gtctgactgg
      541 gaccaagtgg gtaaaataac acttgctata tctttcggcg atgaaacatt agtaacacga
      601 acatcgaagg tgccttgggc atcgttacga ttttcaattg tgatggtacc tgttggctgt
      661 gtaacattga ccgaactgga agctgtactg actcgagcac ttggaacact tgcagagcca
      721 ttcgaacttc ctgcaatcgt atttgaagaa cttcttgtca gcgaattcga agtactactt
      781 gttgcacctg ctgtcaaact agaattaacc gttgagcttg tagtagtcga agttgcttcc
      841 tcctcaattt tagacggagt tgcatcacta ttatccaaag cagtaggctt ctcagccgcg
      901 cttacgacat ccttcttcca ctcgaaacta taatctgata aggtctgaaa acttccatct
      961 gttaattgtg ctgttaactt cagcgaaaag acttggtctt cctccgcaaa tgcagaccga
     1021 tccaaaaccg ctacgtatct accctgctca tccattgaga atgtgacagt aatgccgcta
     1081 tttgtttcag tgctaaactg ggctgtaaca ccaataattt ccccttgaat gttcgataga
     1141 atagctttaa tctgaccagc ctcatcaacg acagataact gcccgccagt ttcagcaacc
     1201 tgagatgaag aaacttgact agatggggca tttaccatat ccgcttgaac aagctgagca
     1261 ttcaacgcgg atgcacacaa agaaagaaat aataacgttc tttttttcac aaacatctcc
     1321 tttatgtatt tcacatgtaa aaacaactat atttctcaaa atagatgcaa ttacactaat
     1381 cttctatttt tcttcttgct tctttgtcaa taaccaacca gcaaaaattg ttatgagtcc
     1441 tagtacagtt aatggaagac tagatttccc tgaagttttt ggcaatacct tagcattatc
     1501 atttgggata acctttttct gacctgcttc ggaaatattg cctttgcctg tagttacatc
     1561 agaaatctga ccgccccctt gacctggaac atcctgctca tttgagccat ttgtagcgct
     1621 ttgacttgtt tgtaccccgg tattttcagt cttagctgta cttttatcct cgacttttga
     1681 ttgtagacta gcagttagtt ctgcaatctg tcggtccatt tctgaaaagt catacggaaa
     1741 ttttggttgc gaagcagttg caagagcttg accatatcct atagttgcag atgtaataga
     1801 ttctaaatta gagtttccac tttccaataa tccagctaat tcttgtccag ttgcttctgc
     1861 ataagcaata taattatcaa tcgctgccga ctctttattc agtagttcaa cgataacttc
     1921 tctagcgtat ttcaatgcag ccaattcggc ttcaggagta cttgcatcat cttcagactc
     1981 gtacttagaa actatagccg ccgcttcttc tcttccgctt tttccagcga ctgtaagtgt
     2041 ctcaaataag taatggtaat gttgggctat gctaccaatt aaatttgctt cgagttcatc
     2101 taaaacaaca acttgctctg ctgttaagtt tgccttcaag tctgcaattt cttcatccca
     2161 cgcagcttca gcactacccg aagccatttc ttccatcatt ccctcaagaa acgccaatct
     2221 atattcttgc atatcgatag tcattttagc ttgaatatat tcacgtacgg caactttttc
     2281 atcatcagtt aaatgatagg tcgtactttg acttgtctct gctgctacaa ctggaacagc
     2341 tccggacggt agtgtagcac ttcctagtaa agccagaaaa attcctgaca tcaacaacgg
     2401 cttaacaatc gccttctttt tcatacacat ctcctgtaga ctatttcttt tcttgtttat
     2461 agaatgcttc caatgcttct tgccaagttg ggatgacaaa acctgtcgcc tttgccttgt
     2521 ccaagctcat ggttgagttg aatggacgtt ttgccttggc tgggaactgg ctagaatcca
     2581 ctggcaatac cttggtgttg gtatccttga ggatttccgt cgcaaagtca aaccaagtcg
     2641 tatcttctgc tgcatcgttt gacaaatggt agtaaccaaa tgcttgcttg gtgtccacca
     2701 agtgtaccat gaattccgca agggtacggg tccaagttgg acgaccgtgt tggtcattga
     2761 caaccgtcaa agtatcgcgg gtttccgcaa gattttgcat agtgaaaaca aagtttttac
     2821 cgtagttacc aaagacccaa gctgtacgaa cagtgtagaa tttgtcagca aatttctcaa
     2881 ctgcttcctc acccttgcgc ttggtgcgac cgtattcaga ttgaggatcc ggttggtcat
     2941 ctacttgcca ctctttgccg actggcaaat caccattgaa gacatagtcc gttgagatgt
     3001 aaacgagggt cgcacagtat ttagcggctg ccttggcaac attttcagag cctgttacgt
     3061 taatcttgta gttgagttcc ttgccttcat cctctgccat atcaacagct gtgtaggctg
     3121 cacagtggta tacaacgctt ggctttactt ctgcaaagaa ggcatctact ttatctgcat
     3181 ccgtgatgtc catttctgct acgtcagcgg caacgtaatc aacaccacgc tcatccaaga
     3241 gataacgcaa ttctgtaccc aactgaccat ttgcacctgt aattaaaatc atatttgctc
     3301 cttatttcaa gaaaggcttg ggcaagagcc ctagccttta ataatcttat ttaataactt
     3361 cctgtgtctt agcatagttg gcttcaacag cttctttttc agctttccac cagtcttggt
     3421 tatctgtata ccactggata gtctcttcca aaccttgaga gaagtctgtg aattgtggtg
     3481 tccaaccaag ctcgtcacga agtttgcttg catcaatagc atagcgcaaa tcgtgtcctg
     3541 cacggtcagt cacgtggtcg taagcatctt ttggttgacc cattttttca agaatcaatt
     3601 caagcacttc tttgttgttc ttctcaccgt cagcaccgat taggtaagtt tcacccatac
     3661 ggccttttgt caagattgcc caaacaccag tcgagtggtc gttggtatga atccagtcac
     3721 ggacgttttt accttcaccg taaagttttg gcttaatacc tgccaagata ttggtgattt
     3781 ggcgtgggat aaatttctcg atgtgttggt aaggaccgta gttgtttgaa cagtttgaga
     3841 tagttgcttt gacaccaaat gaacgcaccc atgctttgac aatcaagtct gaagcagctt
     3901 tggttgatga gtaaggtgat gacgggttat agttggtttc cgcagtaaat ttctcgcctg
     3961 gaccttcgcc atgacctggc aaatcttcac gcaaaggaag gtcaccgtat acttcgtcgg
     4021 tcgatacatg gtggaaacgg atgtcgtatt tacgagccgc ttcaagcaaa gtgtaagtac
     4081 cgataaagtt ggtatggata aatgggcttg gatcgttgag agagttgtcg ttgtggcttt
     4141 ctgccgcata gtgaacgatg gcatctgcct tagcagccaa tttgtcaacc aactcagcat
     4201 ctgcaatgtc gccaacaacc agctcaacac ggtcaccaag aatagcttcc aagttagcct
     4261 tgtttcctgc ataagtaagt ttatccaaaa cagttacgtg aacgtctgga tgattgttat
     4321 atacatagtg tacaaagttt gaaccgatga aaccagctcc accagtcacg ataatatttt
     4381 taaattgaga catttttttc tccgaaaagt tttttctttt ttgtgtgtca tcttaggttg
     4441 tggggtcgta agcaaccacc aaggtgtttc attactaaac catgagccta aggtcccatg
     4501 gtttcccccg ctccttagca aagcaaagga gcttctcact acggcgatga cacacagaac
     4561 tgctcgcttc gctcgctttt ttacctcaag atttccttca agtaagcaat cttagcaaag
     4621 tctttttgat tgttattttc tgcacggtag gaatcttttg aagaggcttg gtagattttc
     4681 aggtattgtt caagtgtcgg taggtaatag cgaacaccta ctgtttctag ttcttccaaa
     4741 tcttctagct ctacaccagc aaattcaggc agtgaatgta ggcctccgaa ttcaacagat
     4801 aagccgtctt tatgaaattc atgttcatga cgatcaacca ggacatagcc aagttgattc
     4861 atcagaacaa tcagctcatc tgcacggtaa atccgctcct catctggcgc ttcccatccc
     4921 cgtgggtcac tgggcacatg aatatcaaga tcccttgcct gccaatctcg tcctgtccgc
     4981 ctttccagac cgacagaacc cattaacaag ggagtgattc ctaacttgtt taactgctct
     5041 gctatttcca aaaaggcttg aaacattaga ggtcctcttt tctcaaaggc ttcacatctt
     5101 tgaggagtgg gtgattttta tctgcttctg aaacttctgc ttcttctagg ttttcccact
     5161 ggatgtcaag gctcgggtca gcgtagttga cgaaggcata cttaggctta agctccaaag
     5221 cccagtagtc gttgaccaag tagctgtaag ccacgaaatc tgacaagact tggaaaccat
     5281 tggctactcc gcgcggaacg aagatacctt ttgaagcatc gataacggtt tggtaggtat
     5341 taccgaaggt ttcgccttca cgtagatcga cccaagttcc caaaacctta ccgccgtctg
     5401 ctactgaaat gtatttatcc caaggctctg cgtgaagacc acgaagaacg tttttacgag
     5461 agaaggaaac attattctgc aattttcctt ctgcaaagaa gctttcagga aagccgagag
     5521 gcaacatttt ttccttttgg aagttctcct tgaaccagcc acggttgtcg ccatgaacag
     5581 gaatgtcgaa ttctaacatt cctggaatag cttcaactgg gcgagctgct agtgttttac
     5641 cgaaaaagtt ttctgtcatt aagcttctcc aatcaaacgg agcaagtatt gtccgtattc
     5701 atttttctta agcggttgtg ccaattcata cacttgttct tttgtaatgt agcccatgcg
     5761 gtaagcaatt tcttccaagt tagccacttg aacgttttgc aaacgttgaa ccgtttcgat
     5821 gtactgtgca gcttccaaga ggctttcatg cgtacctgta tccaaccaag caaatccacg
     5881 gcccatcaat tcaactgata aatcacctcg atctaagtaa gccttgttga catctgtgat
     5941 ttccaactcg ccacgaggtg aaggcttgat atttttcgca atctccacaa catcattgtc
     6001 atagaaataa agaccagtaa cagcaaaatt cgattttggc tcttcaggtt tttcttcgat
     6061 agagatggca ttcatatcag cgtcaaactc aacaacacca aaccgttctg gatcttttac
     6121 ttgataacca aagacagtcg ctcccttttc tttgctagca gcacgttgca acatcttggt
     6181 caaaccattt ccgtggtaga tgttatcacc aagaatcaag gctacattgt catcaccgat
     6241 aaattcttca ccgataataa aggcttgcgc caaaccatct ggacttggtt gttctgcata
     6301 tgaaagtgaa attccaagtt cagaaccatc tcctaacata tccttaaaac gtggaagatc
     6361 ttgcggagtt gaaataatca aaatatcttt gattccagcc aacatcaatg ttgacaatgg
     6421 ataatagatc attggtttat cgtaaattgg catcaactgt tttgaagctg cgcgcgtcaa
     6481 aggatacaag cgtgtacctg acccaccagc tagaataata cctttcatat aagagtacct
     6541 tccctaaatt aatatcagtt cattatacca tatttttaaa gatattgtca taagaaaaaa
     6601 gcctgtgacg acagacttta acaaattatc ctagagcaac atccaaaatc atcatgatga
     6661 taaaaccagc cattaagccc agagttgcaa tatccgtatt gccgtttgtc tgcgattctg
     6721 gaataagctc ctctacgacg acaaaaatca tggctcctgc cgcaaaggag agggcataag
     6781 gtaaaatagg tgtcataaag gtgaccgcaa aagccccgat gactgctgca atcggctcga
     6841 caattgctga cattgatccc cagtaaaaag ctttccacct agatgcgccg tctgtacgaa
     6901 ttggaatagc taaggctgca ccttcaggaa tattttgtat accgatccca atagcaagac
     6961 cgattgcgcc gataaaagct gctggactat agttagaagc tagtgcacca aatgtcacac
     7021 cgaccgccaa gccttcagga atattgtgaa ttgtaatagc taagaagagc agggctgttt
     7081 tggatagatt ttttctatcc ttctcaccac caccttctgc cttatcttta tcatttccca
     7141 aatgcaaatg cggaacccag gcatctacca agcgtaaaaa aataccacct gctgcaaaac
     7201 ctactgctgc tggtatccaa gccagattac cgtatgaact ttcagcatac tcaatcgatg
     7261 gggctaataa agacaaaaag aagccgcaat cataacacca gctgcaaagc ctagcatggt
     7321 atctagcaaa cgacgactaa ctgtcttaaa gaaaaataca acggcagacc ccataatcgt
     7381 acaaaaccat gtgaataaac cacctagcaa agcctgtaga actacagact gattagtaac
     7441 ccaagtcata actaacatcc tat
//
LOCUS       DAZQCJ010000093         7448 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000093 DAZQCJ010000000
VERSION     DAZQCJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7448
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.094"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..1481
                     /locus_tag="ACHU66_001748"
     CDS             <1..1481
                     /locus_tag="ACHU66_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFR3390014.1"
                     /translation="PFRFFNKEENSDSFNNQWGWSAGQAPNEMINNWKTGNRFSKIYF
                     IDKITDTRQVTYTLTASVTDSNQKSFPLVAVMKSFWYTNSQGTQITSLAGREITLEKE
                     KPLPPKETPAPEAPSTPEKQPESPKEDPKKEDTPEVSPKPEAPTPDAPKQDVPETPDK
                     QPEEMPKVPEAPKEDAPAPAPSTPSVPEEKPKEDSKPEVPSAPEAPQQDDVQPDAPQV
                     PEAPQQDDVQPDAPQVPEVPKQDDVQPDAPQVPEAPKEEVPTPPAPSVPEEQPKETPT
                     PEVPKQDDVQPEAPKSDKVETDKQMPETKQPDMKQPKADDMPKEQKPKADEPKVEQPQ
                     MEAPKKDSEAPKSDKVESDKQLPETKQPDMKQPKADDMPKEQKPKADEPKAEQPQMEA
                     PKKDSEAPKSDKVETDKPMPETKQPDMKQPKADKPEAEKAQMPRTEGMKPESKASMMP
                     KAEAPKATLPNTGEASSAIGWLGGALATLATGLYLFKNKKEE"
     gene            1917..3506
                     /locus_tag="ACHU66_001749"
     CDS             1917..3506
                     /locus_tag="ACHU66_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFR3390015.1"
                     /translation="MSKQKVVTNLLLSTAVLGGFLICHVSSVSAETGNLQEIPTESAT
                     VSSENQTVTAVDGISPTDNQLPTSEEGDEQAPEEKLVSSDSTREESATQVATDPDAPS
                     QSLSVSGAEISTADSNIQQAEEKLIQESNYHRFDTHQGEQIRKAVSIEKISADNDEIK
                     WKVTFDRKNWTFSGEGSGYYFILLKGLQLTKIIDSQSEEDIFNKFPKDVNSEANSGGQ
                     PYRFFSREKNANNDERSFESQWGWSVGRVGGELEKWKEESSSVYFLQVQRDSVAKEKA
                     TYELTAQVTNREMQTFPVLAVVKNFKERIFFSRDEITSAAGLRLKVSESAKPNNEGIT
                     IIGDSSPESDRTPEPRGDRTSDIVVPKADVPKVEQPKPKDPEVQLPAPKAPESVPPKV
                     MPPAPKVPDVKTPKEAPLPPKTPEVEVPKVMPPTPKVPDVKTPKVEYPDTDAPVIESP
                     KAPETERPEVLPPTPKVPEVEVPKVIPPTSKVPEAELPKVEQPKPKVPEVHLPAPKSP
                     ETEPPKVMSPAPKVPDVKTPK"
     gene            3780..3926
                     /locus_tag="ACHU66_001750"
     CDS             3780..3926
                     /locus_tag="ACHU66_001750"
                     /inference="COORDINATES: protein motif:HMM:NF012948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFR3390016.1"
                     /translation="MAPKVGIQNETLPNTGETSNTLVWLGGALATLASSLYLFKQHRQ
                     REEE"
     gene            complement(4397..4546)
                     /gene="rpmG"
                     /locus_tag="ACHU66_001751"
     CDS             complement(4397..4546)
                     /gene="rpmG"
                     /locus_tag="ACHU66_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR3390017.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVI
                     FTEVK"
     gene            complement(4562..4747)
                     /gene="rpmF"
                     /locus_tag="ACHU66_001752"
     CDS             complement(4562..4747)
                     /gene="rpmF"
                     /locus_tag="ACHU66_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR3390018.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVAAPTVKFDETTGDYSRSHRVSL
                     KGYYKGRRKIAKAASAE"
     gene            5023..6306
                     /gene="hisS"
                     /locus_tag="ACHU66_001753"
     CDS             5023..6306
                     /gene="hisS"
                     /locus_tag="ACHU66_001753"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR3390019.1"
                     /translation="MKLQKPKGTQDLLPQDSAKWQYVENFTRSIFKQYNYAEIRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGERHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PAKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSNNPATDVETIAMAYHFFEELGIKD
                     IRLHLNSLGNPESRAAYRQALIDYLTPLKDQLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDYLDEESTAHFEAVKTMLDSLGIAYVIDTNMVRGLDYYNHTIFEFMTEVG
                     GNDLTICAGGRYDGLVTYFGGPETPAFGFGMGIERLILVLEKQGIELPLDTQLDVYIA
                     VLGQEANGGALDLVQAIRKQGFRAERDYLDRKLKAQFKSADVFGAKAIITLGGSEIES
                     GQVVVKNNQTRSQVETSLEALKTDFASILEELEKQ"
     gene            complement(6387..7412)
                     /gene="adhP"
                     /locus_tag="ACHU66_001754"
     CDS             complement(6387..7412)
                     /gene="adhP"
                     /locus_tag="ACHU66_001754"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFR3390020.1"
                     /translation="MKAVVVNPESTGVVVVDKELRSLEAGEALVQIEYCGVCHTDLHV
                     ANGDFGKVPGRVLGHEGIGIVTEIAPGVTSLKVGDRVSVAWFFQGCGMCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPEGLDPAQASSITCAGVTCYKAIKEAHL
                     EPGQWIAIYGAGGLGNLAVQYAKKVFNAHVIAVDINNNKLELAKEVGADVTINGLEVE
                     DVPGYIKEITGGGVHSTVVTAVSKVAFNQAIDSVRAGGYVVAVGLPSEYMDLSIVKTV
                     LDGIKVVGSLVGTRKDLEEAFHFGAMGLVVPVVQKRPVKDAEAVFNEMVAGTIQGRMV
                     LDFCHSH"
BASE COUNT         2455 a         1582 c         1594 g         1817 t
ORIGIN      
        1 caccattccg tttctttaat aaagaagaaa acagcgatag ctttaataat caatgggggt
       61 ggtcagcagg gcaagctcct aatgaaatga ttaataactg gaaaacagga aatcgttttt
      121 ctaagattta ctttatagat aaaataacag atactagaca ggtaacctat actttaactg
      181 ctagtgtgac tgattcaaat caaaaatcat tcccacttgt agcagtgatg aagagctttt
      241 ggtatacaaa ctcgcaagga acgcagatta cttcattagc tggtcgtgag attacccttg
      301 agaaagaaaa acctctacca cctaaagaga ctccagcgcc agaagctccg tcaactcctg
      361 aaaagcaacc ggaatctcca aaggaagatc ctaagaagga agatactccg gaggtttcac
      421 caaaaccaga agcgccaaca ccagacgcac caaaacagga cgttccagaa actccggata
      481 agcaaccaga agaaatgcct aaagttcctg aagcaccgaa ggaagacgct ccagctccag
      541 cgccatcgac tccatcagta ccagaagaaa aaccaaagga agattcgaaa ccagaagtgc
      601 catcagcacc ggaagcacca caacaagacg acgtccaacc agacgctcca caagtaccgg
      661 aagcaccaca acaagacgat gtccaaccag acgctccaca agtaccggaa gtaccaaaac
      721 aagacgatgt ccaaccagac gctccacaag taccggaagc accgaaggaa gaagttccaa
      781 ccccaccggc tccatcagta ccagaagagc aaccaaaaga aactccaaca ccagaagttc
      841 caaaacaaga cgatgttcaa ccggaagcac caaaatctga taaagtagaa actgacaaac
      901 aaatgccaga aactaagcaa ccagatatga agcaaccgaa ggcagacgac atgcctaagg
      961 aacaaaaacc gaaagccgac gagcccaagg tagagcaacc acaaatggaa gctcctaaga
     1021 aagattcgga agcaccaaaa tctgataaag tagaatctga caagcaattg ccagaaacta
     1081 agcaaccaga tatgaagcaa ccgaaggcag acgacatgcc taaggaacaa aaaccgaaag
     1141 ccgacgagcc aaaggcagag caaccacaaa tggaagctcc taagaaagat tcggaagcac
     1201 caaaatctga taaagtagaa actgacaaac caatgccaga aactaaacaa ccagatatga
     1261 agcaaccgaa ggcagataaa ccagaagcag agaaagctca aatgccacgg acagagggta
     1321 tgaagcctga atctaaggct tcaatgatgc caaaagcaga ggcaccaaaa gcaactttgc
     1381 caaatacagg cgaagcaagt agcgcaatag gctggctagg tggtgcttta gccactcttg
     1441 ccacaggctt gtatctattc aaaaacaaaa aagaagaata gtcattattg aataagatat
     1501 gagaacttcc ctcttctaag ttcataaaag actccagatc atcgctgtag aagccttggt
     1561 ttggagtctt tttgttaaat attcttagaa aataacaaaa tagataaata ttagataccc
     1621 tctagaaaaa atttagttta ggatgttata ctaacgctaa aacaataata tcaatttttg
     1681 aaaatactag attggaataa aaatctaatc gccatatttt agaacctgta cttacaagta
     1741 aagagctttt atgtaagaga tagtttttcg ctaatcaagt taattttttg agggaatgct
     1801 ggctgtattt tgaaaaaaac gaacgatgag tagcttaaaa atgccttaga tgggagtgct
     1861 aacctgtaaa tacaggttct tagtaaattg aagtaaatag agtaaaggag cacattatga
     1921 gcaaacaaaa agttgtgaca aatctattgc ttagtacagc tgtactaggt ggttttctaa
     1981 tatgtcacgt atcatctgta agcgcagaaa caggcaatct tcaagaaata cctactgaat
     2041 ctgctacggt tagctcagaa aatcaaactg tgaccgcagt cgatggaatt tcacctacgg
     2101 ataaccaatt acctacgtca gaagaaggtg atgaacaggc tccagaagaa aaactagttt
     2161 ctagcgattc tacgagagaa gaaagtgcta cgcaggtagc gacggatccg gatgcgccat
     2221 ctcaatcatt aagtgtgtca ggggcggaaa tttcaacggc agattcaaat attcaacaag
     2281 ccgaagagaa actgattcaa gaatcgaatt atcatagatt tgacactcat caaggtgaac
     2341 agattaggaa ggcagtatct atagaaaaaa tatcggcaga taatgacgaa attaagtgga
     2401 aagtaacttt tgatagaaaa aattggacct ttagtggaga ggggagtggc tattacttta
     2461 ttcttctaaa aggcttacaa ttaaccaaaa ttattgatag ccagtctgaa gaggatattt
     2521 tcaataagtt ccctaaggat gtcaatagtg aggcaaattc tggcgggcag ccgtatcgct
     2581 tttttagtag agaaaaaaat gctaataatg atgagcgtag ttttgaaagt cagtggggct
     2641 ggtctgttgg acgtgtagga ggagagctag aaaaatggaa ggaagaaagt tccagcgtct
     2701 attttctaca agtacagcga gattccgttg ctaaagaaaa agcgacatat gaactgacgg
     2761 cacaggtgac aaatcgagaa atgcagactt tcccggtttt agctgtggtg aagaatttta
     2821 aagagcgaat atttttctcc agagatgaga ttacttctgc tgctggtttg agattaaagg
     2881 tatcggaaag tgctaagcca aataatgaag ggattactat tatcggagat tcatctccag
     2941 aaagcgacag gactcctgaa ccgcggggag ataggacttc ggatatagtt gtaccaaagg
     3001 cagatgttcc aaaagttgag caaccaaaac caaaggaccc tgaagttcaa ctgcctgctc
     3061 ctaaggctcc ggaatctgtg ccaccaaaag taatgcctcc agcacccaaa gttccagacg
     3121 ttaagacgcc caaagaggcg cctctgccac caaaaactcc ggaagttgag gttccaaaag
     3181 taatgcctcc gacaccgaag gttccagatg ttaagacgcc aaaagttgag taccctgata
     3241 cagatgctcc tgtgattgag tcaccaaaag ctcctgaaac tgaacgacca gaagtcttac
     3301 caccgacgcc aaaagtccca gaagttgagg ttccgaaagt aattccaccg acctcaaaag
     3361 ttccagaagc tgagctaccg aaagttgagc agccaaaacc gaaggttcca gaagttcacc
     3421 ttcctgctcc taagtctccg gaaactgagc cacccaaagt aatgtcccca gcacccaaag
     3481 ttccagacgt taagacgcca aaataggcgc ctctgccacc aaaaactccg ggcgttgagg
     3541 ttccgaaagt aattccaccg accccaaaag ccccagaagt tgaggttccg aaagtaacac
     3601 caccaacctc aaaaattcca gacgttaaga cgccaaaatc tccggaagtt gcgcacccag
     3661 aagttgagaa acccgccgga atccaaccag aaaagggtca acctgaggtt aataaaccca
     3721 atattattca ttcggaggtg cttcaatccg aagttaagaa aacggaatct ggagtttcaa
     3781 tggctccaaa ggtaggaata caaaatgaga ctttaccaaa tacgggtgaa acgagcaaca
     3841 ccctagtatg gctaggcggt gctctagcta cccttgcatc aagtttgtat cttttcaaac
     3901 aacacaggca aagagaagag gaatgatttc acaccaatat tatggttttt ggaacgccaa
     3961 taaggcttat ctaccatgga ctaagaaaat ttagaacagt tgtaaataga aaacgttcca
     4021 aaatctgaac tgcaccccaa aagttagagg ttaactctaa tttttgaggt gcttttcttg
     4081 taaggtcaag acacaaagac aagattgaaa tttatcgctt gcaatgatat gaaggagttg
     4141 gttctatatt ttgttttatc aatactagtt tgaatgttat tcgagaattt gtaggacatt
     4201 ctgaggatgg taaggagata gtgaaaatct atcttcatgt aatacaaaaa agaaaacata
     4261 agattgaaca ggctatgcta gatttagcag aaataattat ttgatttatt ttaatcagtt
     4321 aaaattcagt tatcttgtta aaaacaaaag aaaaagcctt tgaaacgttg atttcatagg
     4381 ctttctgtat gttgtcttat tttacctcag taaaaattac gtgcttacgc aattttggtg
     4441 agtatttttt caattgaaga cggtctggag tgttacgttt gttttttgaa gtaaggtaca
     4501 aacgctcacc agattctttg tgttcaagtg taatatttac gcgcatggta tctcccttct
     4561 attattcagc tgaagcagcc ttggcaattt tacgtcgtcc tttgtagtat cctttcaaag
     4621 atacacggtg tgaacgtgag taatctccag tagtttcatc aaatttcaca gttggagctg
     4681 ctactttata gtgagtacga cgtttgtttt tcttcgcttt tgaagtgcga cgtgcaggta
     4741 ctgccatttc tttcttcctc cattaaatgt cagggctcag ccccttataa atcaaaaaat
     4801 agtgcgtatg actttcgcca actttaacag tataacaaaa gttttttgta aagtaaagtt
     4861 acttgacaga tttttttcac ttttttggaa aatagcagag catttatggt aaaataagga
     4921 ggactgcaag gctcgactgg tagagtttga gcccttatga cttaatttgg aaaggaagaa
     4981 gcacaacttc gagcgatata agttggctct atacacttta ttatgaaact acaaaaacca
     5041 aagggaacac aggatttact accgcaggat tctgccaaat ggcagtacgt ggaaaacttt
     5101 acccgcagca ttttcaaaca gtacaattac gctgagattc gcacaccgat cttcgagcat
     5161 tacgaggtga tcagccgttc ggttggggat acgacagaca ttgtgaccaa ggagatgtac
     5221 gatttctatg acaagggaga gcgtcatatc accctccgtc cagaagggac ggcacccgtt
     5281 gttcgttctt atgtagaaaa taagctcttt gctccagaag tgcaaaagcc agctaagttc
     5341 tattacatgg gtccaatgtt ccgctatgag cgtccgcagg caggtcgtct ccgtcagttc
     5401 caccagattg gtgtggagtg ttttggttct aataacccag cgacagacgt agaaaccatt
     5461 gctatggcct atcacttctt tgaagagttg gggatcaagg acattcgcct ccacctcaac
     5521 agtctgggca atcccgagag ccgtgctgct tatcgccagg ctttgattga ctatctgacg
     5581 ccgctcaagg accagctttc caaggatagc cagcgccgtc tagaagaaaa tcctttgcgt
     5641 gtgttggatt ccaaggaaaa agaagacaag gttgctgtgg aaaatgcacc gtccatcttg
     5701 gactatctag atgaagaaag tactgctcac tttgaagcgg ttaagactat gttggacagc
     5761 ttaggcattg cttatgtgat tgacaccaac atggtgcgtg gtttggacta ctataaccat
     5821 actattttcg agtttatgac cgaggtaggt ggcaatgacc tgaccatctg tgccggtggc
     5881 cgttatgatg gcttggtgac ctactttggt ggaccagaaa cgccagcttt tggctttggc
     5941 atgggcattg agcgcctcat cctagtcctt gaaaagcaag gcatcgagct ccctctcgac
     6001 acccagttag atgtttacat cgcggttttg ggtcaggagg ccaatggcgg agcgcttgat
     6061 cttgttcaag ccattcgcaa gcaaggtttc cgtgcagaac gtgactatct ggaccgcaag
     6121 ctcaaggccc agtttaagtc agcagatgtc tttggagcca aggcaatcat taccctgggt
     6181 ggtagcgaaa tcgaatctgg acaggttgtg gtcaaaaata accagacaag aagtcaagtt
     6241 gaaacaagct tggaagcact taaaacagat tttgcaagta ttttagaaga attggagaag
     6301 cagtagtcta aagctgcttc tttttatttt agaaagcaaa aaagaagcga ttggataatc
     6361 acttcttgaa aatagtggag aacgcattag tgtgagtggc agaagtctaa gaccatacga
     6421 ccttggatag taccggcaac catttcattg aagactgctt cggcatcttt gacagggcgt
     6481 ttttgaacga ctggaacaac tagtcccata gcaccgaagt ggaaggcctc ttctaaatct
     6541 ttacgagtac caactagtga gccgaccact ttgataccgt ctagcacagt cttcacgata
     6601 ctgaggtcca tgtattcaga tggaagtccg acagccacca catagccacc agcacgaaca
     6661 ctgtcaatag cttggttgaa ggctactttg gaaacagctg ttacaacagt agagtgtaca
     6721 ccgccaccag taatttcttt aatatatcca ggaacatctt ctacttcaag tccgttgatc
     6781 gtcacatctg caccaacttc tttggcgagt tccaatttat tattattgat gtctacggcg
     6841 ataacatgag cgttgaagac ttttttagcg tattgtacag caaggtttcc aagtccacca
     6901 gcaccataga tagcaatcca ttgacctggt tccaagtgag cttctttaat cgctttgtag
     6961 caagtaacgc cagcacaagt gatagaactt gcttgagctg gatcaagacc ttctggaact
     7021 tttactgcat agtccgctgt tacaatacat tgctcagcca taccaccgtc aacagagtag
     7081 ccggcatttt ttacggtacg gcagagagtt tcgcgaccgg ttgtacaata ttcacacata
     7141 ccacagcctt ggaagaacca agcaacgctg acacggtcac caacttttag actggtaaca
     7201 ccaggtgcga tttctgtaac aataccgatt ccttcgtgac caagaacgcg accagggacc
     7261 ttaccaaaat caccattggc aacgtggagg tcagtgtgac aaacaccaca gtactcaatt
     7321 tggaccaaag cctcaccagc ttcgagtgaa cgaagttcct tatcgacaac gacaacacca
     7381 gtagattctg gatttacaac gactgctttc ataagcagac ctcctgtttt aatattttat
     7441 atacattt
//
LOCUS       DAZQCJ010000094         7328 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000094 DAZQCJ010000000
VERSION     DAZQCJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7328
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.095"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(789..1637)
                     /locus_tag="ACHU66_001755"
     CDS             complement(789..1637)
                     /locus_tag="ACHU66_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFR3390021.1"
                     /translation="MTVIDGKALGVKLQAALAEKTARLKEEKGVVPGLVVILVGENPA
                     SQVYVRNKERSALAAGFKSEVVRVPDTISESDLLDLIERYNQDDEWHGILVQLPLPAH
                     ISEEKVLLAIDPDKDVDGFHPTNMGKFWSGHPVMIPSTPAGIMEMFKEYQIELEGKSA
                     LVIGRSNIVGKPMAQLLLDANATVTIAHSRTKNLPELARQADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDENGKLIGDVKYDEVSEVASYITPVPGGVGPMTITMLMEQTYEA
                     CVRSAK"
     gene            complement(1767..2501)
                     /locus_tag="ACHU66_001756"
     CDS             complement(1767..2501)
                     /locus_tag="ACHU66_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR3390022.1"
                     /translation="MSNAIISIKDLHKYFGKNEVLKGIDLDIQQGQVVVIIGPSGSGK
                     STFLRTMNLLEVPTKGTVTFEGVDITDKSNDIFKMREKMGMVFQQFNLFPNMTVLDNI
                     TLSPIKTKGIAKDEAEKKAKELLEKVGLPDKANAYPQSLSGGQQQRIAIARGLAMDPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMDGGII
                     VEDGTPEEVFEHTKEERTKDFLSKVL"
     gene            complement(2494..3183)
                     /locus_tag="ACHU66_001757"
     CDS             complement(2494..3183)
                     /locus_tag="ACHU66_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR3390023.1"
                     /translation="MNFSFLPEYWAYFNYGAIVTLIIAFFSVFFGSILGVLLAFAQRS
                     KYKVLAWTANIYVWIFRGTPMIVQIMIAFAMTHFVAPTFQLGILTVDLTRLIPGIIVI
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKQAMRYVIMPQAIKNILPALGNEF
                     VTIVKDSSLLSTIGVMELWNGAQTVQSTSYIPLTPLVFAASYYLIMTTVLTVIIQSFE
                     KKLNKGGQFHV"
     gene            complement(3309..3539)
                     /locus_tag="ACHU66_001758"
     CDS             complement(3309..3539)
                     /locus_tag="ACHU66_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HFR3390024.1"
                     /translation="MESHLVRIINRLEAMANDGGTLKRNFERDGIVVAEVAYSYDEEN
                     GSVFTLRDVAARETYAFDSIDLIAMEIYELLY"
     gene            3763..5991
                     /locus_tag="ACHU66_001759"
     CDS             3763..5991
                     /locus_tag="ACHU66_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR3390025.1"
                     /translation="MLCKNCNINDATIHLYNNLNGKQQQVDLCHNCYQIMKTDPNNAI
                     LRGLGDLTNPNNMDPFSEFFNHLGGYPGNTPAGKNREQTPPTQAGEHNGRGGQTPPPQ
                     QPQQPNGLLEEFGINVTEIARRGDIDPVIGRDQEITRVIEILNRRTKNNPVLIGEPGV
                     GKTAVVEGLAQKIVDGDVPQKLRDKEVIRLDVVSLVQGTGIRGQFEERMQKLMEEIRN
                     RREIILFIDEIHEIVGAGSAGDGNMDAGNILKPALARGEMQLVGATTLNEYRIIEKDA
                     ALERRMQPVKVEEPSVEETITILKGIQNKYQDYHHVKYSDAAIEAAAVLSNRYIQDRF
                     LPDKAIDLLDEAGSKMNLTLNFIDPKEIDQRLIDAENRKAQATRDEDYEKAAYFRDQI
                     AKYKEMQKATISEEDIPLITEKEIEAIVEQKTNIPVGDLKEKEQSQLVNLASDLKAHV
                     IGQDEAVDKIAKAIRRNRVGLGAPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSADS
                     MIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLILLDEVEKAHPDV
                     MHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGTGKVEASVGFGAAMEGRTQSVLG
                     QLSNFFTPEFMNRFDGIIEFQPLSKENLLEIVSLMLDDVNKRLSHNGISLHVTDKVKE
                     KLVDLGYDPKMGARPLRRTIQDQIEDAITDFYLEHPAEKDLRAVMSSKGTIQIKAQTK
                     TK"
     gene            6177..6638
                     /locus_tag="ACHU66_001760"
     CDS             6177..6638
                     /locus_tag="ACHU66_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFR3390026.1"
                     /translation="MEKIAVFGEKKAGVSYQSRFGVYAVIPDEKKEQIILVQAPNGAW
                     FLPGGEIEKGENHLIALERELMEELGFTAEIGKYFGQADEYFYSSHRDTHFYNPAYIY
                     EVTSYHQIGQPLEDFNHIAWFPVNEAIDKLKRGSHKWGIEQWKLQENSFNN"
     gene            6693..7001
                     /locus_tag="ACHU66_001761"
     CDS             6693..7001
                     /locus_tag="ACHU66_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFR3390027.1"
                     /translation="MKIINTTNSHPNLVQSQLANTDAFLVETYSAGNTDVIFTQAPRH
                     YELLISNKYRAVQQNEIEQIRDFFLHRKIDERTINKAAIQTIHTDRLIEMSIPIIAEV
                     "
BASE COUNT         2356 a         1645 c         1305 g         2022 t
ORIGIN      
        1 ttatcctatt attactcaga taaggctagg agtaaaaccg ttccattagc agtttcactg
       61 aaaacgtccc acaatcacct aaaacattat aaacacaaaa tgaaagagag aaagaactaa
      121 acagataata ctcaatgaaa atcagactag gcgacgcaga tgcagataga actgaagttc
      181 atcaaatcaa gtcaacaacg tctgattttg attttcgaag agtataggca ataagtcttc
      241 attcgcagtt gttgagctga aataatctgt ctccaaacgt ttttactaca ctgggatgtt
      301 gttcaaactt tgagaaatag tactgttttt tatacagatt catttttgca tacaaaaaaa
      361 cctgatcaca cgatcaggct tttcaggaga ttatgaaaaa tatttaggat tgggtatagt
      421 ataaccaata ttactaaaag tcacatccgt cttaagactg atttattgaa tatgaccaat
      481 aaaataagta gtgtcgttta aaaatagact gattcatacg gggactgcac tcattactgt
      541 tagctaccaa aactacaagc ataatttaag aacctgtatt cacagttcaa cgcttctatt
      601 ctaaggctag tttttaagct actcatcatt agtttttttc aaaatacaat caatatcccc
      661 caaaaaaatt accttgatta gctaaatctg tctcttaata tgagtacttc gcttgtaaat
      721 acaggttcta agaacagggc tttaccaaag caggcatgga tatatttatt tttattaaac
      781 tctctatctt attttgcgct tctgacacaa gcttcatagg tctgttccat caacatggta
      841 atagtcatcg gaccaacacc acctggaact ggggtaatat aacttgccac ttctgatacc
      901 tcatcgtatt tgacatcacc gattagcttg ccattttcat cacgattcat accaacatca
      961 atcaccacgg cacctggttt aacaaattcc ttggtcacaa aatgaccacg accaatggca
     1021 acaacaagaa tatcggcctg acgagcaagc tctggcaagt ttttggtgcg agaatgcgca
     1081 atcgttacag tagcattggc atccaatagc agctgtgcca ttggtttacc aacaatatta
     1141 gaacgaccaa taactaaagc tgacttccct tccagctcaa tctgatattc cttgaacatt
     1201 tccataattc ctgctggggt agatggaatc ataacaggat gacctgacca gaatttcccc
     1261 atattagtcg gatgaaaacc atccacatcc ttatctgggt caatggccaa caagaccttc
     1321 tcctcgctga tgtgggctgg taagggcaat tgcaccaaaa taccatgcca ttcatcatcc
     1381 tgattatagc gctcaatcaa atccaacaag tctgactcag aaatcgtatc tggaacgcga
     1441 acaacctcac tcttgaatcc agcagctaga gctgaccgct ccttattccg tacatagacc
     1501 tgactagcag gattttcgcc aactaaaata actacaagac ctggtaccac ccccttttct
     1561 tcttttaatc gagcagtttt ctccgccaaa gcagcctgca attttacacc tagtgctttc
     1621 ccatcaatga ctgtcatacc tacactcctt ttgttttttc tattataccc taaaattctc
     1681 cgactgacta tacttgtaca aagtagacta tagcatatag caaaaaactc cgaacaatgt
     1741 cggagtcgta cttatttgtt catttattac aagaccttag acaagaaatc cttggtccgt
     1801 tcttccttgg tatgttcaaa gacttcttca ggcgttccat cctccacgat gataccgcca
     1861 tccataaaga taaccctgtc agctacctca cgcgcaaatc ccatctcatg agtcacgata
     1921 accatggtca tccccgactt ggccaaatcc tgcattacag caagaacttc accaaccatt
     1981 tcagggtcta gtgcagaggt cggttcatca aaaagcagga catctgggtc catggccaga
     2041 ccacgtgcaa tagcgatccg ttgttgctga ccacctgaaa ggctttgtgg ataggcattt
     2101 gccttatctg gcaatcctac cttttcaagt aattccttag ccttcttctc agcctcatcc
     2161 tttgcaattc cctttgtctt aataggtgat aaagtaatgt tatctaatac cgtcatattt
     2221 ggaaaaaggt tgaactgttg aaaaaccatt cccatctttt cacgcatctt gaaaatatca
     2281 tttgacttgt cagtaatatc aacaccttca aatgtaacag ttcccttggt tggcacttct
     2341 aagaggttca ttgtacgtaa gaaagtcgat ttccctgacc ctgatggacc gataataacg
     2401 acaacctgac cttgttgaat atctaaatca attcctttta gaacctcatt ctttccgaag
     2461 tacttatgta aatccttgat agaaataatc gcattagaca tggaactgcc ctcccttgtt
     2521 caactttttc tcaaacgact gaataatcac tgttagcacc gttgtcataa ttagataata
     2581 gctcgctgca aatacaagtg gtgttaaagg aatataagaa gtggattgaa ctgtctgagc
     2641 accgttccat aattccatca caccgatggt tgacaaaagt gaactatcct taacaatagt
     2701 cacgaattca tttcccaagg ctggaagaat attcttgata gcctgcggca tgataacgta
     2761 acgcatcgcc tgctttggtc taataccaag cgaataagct gcttccagct gtccttttgg
     2821 tacagcatta atacctgcac gaacagtttc agatacatag gcacccgagt tcatcgaaat
     2881 aacaatgata cctggaatca agcgcgtcaa atcaaccgtc aaaattccta gctgaaaagt
     2941 tggtgctaca aaatgtgtca tcgcaaaagc aatcataatc tggacaatca tcggtgtccc
     3001 acggaaaatc cagacataga tattggcagt ccaagctaaa actttatact tactacgctg
     3061 agcaaaggca agtaacacac ctaaaatact accaaaaaat actgagaaaa atgcaataat
     3121 gagggtaaca attgccccat aattaaaata agcccaatac tcaggcaaaa aagaaaaatt
     3181 cattcatctt actccaattc tgtcatttag agtctattat accatttttg aatatttatg
     3241 caatactttt gtttataata ttcaatttac acaagaaaaa agcaccctta ggtgcctctc
     3301 acttcttctt agtacaatag ctcatagatt tccattgcaa tcaagtcgat gctgtcaaac
     3361 gcgtacgttt cacgagcagc aacatcacgc aaggtaaata ctgaaccgtt ttcctcgtca
     3421 tagctataag ccacttctgc aacaacaata ccatcacgtt cgaaattacg tttcaacgta
     3481 ccgccgtcat tagccatagc ttccagacga ttgataattc tcaccaaatg tgattccata
     3541 ttcgttctcc taatcctttt tataggaata ttttaccaca tattgccaaa aaagtcttat
     3601 tttttcccta ttttcttcct atcgccctat ttttcataga aagtttttcc aagcgtagca
     3661 gtttcttgat ttttctatga atcttgttat aataaagcta gaactgacct tttttgacta
     3721 tttaacaata ggtcacattt tttcacagaa agaggtagaa ttatgctctg caaaaattgt
     3781 aatatcaacg acgcaacgat ccatctctac aacaacttaa acggtaaaca gcagcaggta
     3841 gacctctgtc acaactgcta ccaaatcatg aaaacagacc ctaataatgc catcctacgt
     3901 gggttgggag atttaacaaa tcctaacaac atggatccct ttagtgaatt tttcaatcac
     3961 ctaggtggct atcctggcaa tacccctgct ggaaaaaatc gcgaacagac tcctcctact
     4021 caagccggtg agcataatgg acgtggtgga cagacccctc ctccgcaaca accccaacaa
     4081 ccaaatgggc tccttgaaga atttggtatc aacgttaccg aaattgcccg tcgtggggat
     4141 attgatccag taatcggtcg cgatcaagaa attacccgtg tcatcgaaat tctcaaccgt
     4201 cgtacaaaaa acaaccctgt tcttatcggt gagcctggtg taggtaaaac agcggttgtt
     4261 gaaggcttgg ctcaaaaaat tgtcgatggc gatgtcccac agaaattacg agacaaggaa
     4321 gtcattcgtc tggatgttgt cagcctcgta caaggaacag gtatccgtgg acaatttgaa
     4381 gaacgcatgc aaaaactcat ggaagaaatc cgtaatcgtc gcgaaatcat tctttttatt
     4441 gacgagattc acgaaattgt cggtgctggc tctgcaggcg atggtaacat ggatgcaggc
     4501 aatatcctca aaccagccct cgcacgtggt gaaatgcaac tagtcggtgc aacaacgctc
     4561 aacgagtacc gtattatcga aaaagatgcc gccctagaac gccgtatgca acctgtaaaa
     4621 gttgaagaac caagtgtcga agaaaccatt accattctca aaggtatcca aaataaatac
     4681 caggattacc atcatgttaa atactccgat gctgctatcg aagctgccgc agtactgtcc
     4741 aaccgctaca ttcaagaccg tttcctacca gacaaagcta ttgaccttct ggatgaagct
     4801 ggttcaaaaa tgaatttaac cctcaacttt attgatccaa aggaaattga ccaacgcctg
     4861 attgatgccg aaaatcggaa agcacaagct acacgggatg aggactatga aaaagcggcc
     4921 tatttccgtg accaaattgc taagtacaag gagatgcaaa aggcaactat cagcgaggaa
     4981 gatattccac tcattactga aaaagaaatt gaggccatcg ttgagcaaaa aacaaatatc
     5041 ccagttggtg atttaaaaga aaaggaacag tcccagcttg tgaacctcgc tagtgactta
     5101 aaagcccatg ttatcggaca agatgaagca gttgacaaga tagctaaagc catccgccgt
     5161 aatcgtgtcg gtctcggtgc tccaaatcgt cccattggat ccttcctctt tgtcggacca
     5221 acaggtgtcg gtaaaaccga attgtctaaa caattagcta tcgaactctt cggttcggca
     5281 gacagcatga tccgcttcga catgtccgaa tacatggaaa aacacgctgt cgctaaactc
     5341 gtcggagctc ctccaggcta tgtaggctat gaggaggctg gacaattgac agaaaaagtc
     5401 cgtcgtaatc cttactcact catcctcctt gacgaagttg aaaaagccca cccagatgtc
     5461 atgcacatgt tccttcaggt cttagacgat ggtcgtctga cagatggaca aggtcggaca
     5521 gtcagcttca aagatactat tatcatcatg acatcaaatg ctggtactgg aaaagttgaa
     5581 gcaagtgtcg gttttggtgc ggctatggaa gggcggactc aatcagtcct tggccaactt
     5641 agcaatttct tcacacctga atttatgaac cgatttgacg gtattattga gttccaacca
     5701 ctgagcaagg aaaacctact tgaaatcgtc agcctcatgc tagacgatgt aaacaaacgc
     5761 ctttctcaca atggcatcag cctccatgta acagataaag ttaaggagaa attagtggac
     5821 cttggctacg atccaaagat gggagctcgt ccactgcgca gaaccatcca agatcagatt
     5881 gaagatgcta tcaccgactt ctacttagaa catccagcag aaaaagacct acgtgctgtc
     5941 atgtcaagta aaggtacaat tcaaatcaag gcacaaacaa agacaaaata agaaaataga
     6001 gctgctccct acgagtagcc tattttactg ttttgctact ctatttttct aaattcttcc
     6061 ccatctccat cctataaaaa aactttactt tgtaaagcct atcacaagga aaaataataa
     6121 aaaagtggta taatgtatac aactcattcc aataatgaaa gaggatagaa ttgcctatgg
     6181 agaaaattgc tgtatttgga gaaaagaagg ctggcgtcag ctaccagagt cgctttggtg
     6241 tttacgcagt tatcccagat gaaaaaaaag aacagattat actcgttcaa gcaccaaacg
     6301 gagcttggtt tcttcctggt ggagaaattg aaaagggaga aaatcacctt attgctttgg
     6361 agcgcgaatt gatggaagag cttggattca ccgctgaaat cggaaaatac tttggtcagg
     6421 ctgacgaata cttctactct agccaccgcg atacccattt ctataatcca gcctacatct
     6481 atgaagtgac tagctaccat caaataggac aaccactaga agatttcaac catattgctt
     6541 ggttcccagt caatgaagcc atcgataaac tcaaacgtgg tagtcacaaa tggggcattg
     6601 aacaatggaa attgcaagaa aattcattca ataactagta aaaacactct aatcgtgcta
     6661 taatgtagcc aaagagatag aggaggttcc acatgaaaat cattaacacg acaaatagtc
     6721 atcccaatct tgtccaaagc cagttggcta atactgatgc tttcctagta gagacttatt
     6781 ctgcaggtaa tacagacgtt attttcacac aggcaccccg tcattatgaa ctcttgatta
     6841 gtaataaata ccgtgcagta caacaaaatg aaatcgagca aattcgtgat ttcttccttc
     6901 atcggaaaat tgatgaacga actattaaca aggctgccat tcaaacgatt cacacagacc
     6961 gtttgattga aatgtctatt cccattatcg cagaagtgta agaagccaga tggcttcttt
     7021 cagattgaag aaaaagtcca ttttggacaa ttttcttcaa tcttttttct ttatattgaa
     7081 aagaaaaact ctcagaaacg ctgaaatatc aatgtttcca agagtttaat cacttgctat
     7141 ctttcgcaaa cctcttcaat tttttatatt tttaggagtt tgtctacgtt ctgtaagaag
     7201 ccaattggct tctttttttc tatttatact caatgaaaat caaaagtagc ctaggaaacg
     7261 aagccgaaga tagaactgga gttcatcaag gctcttgaca acgaataatt ttgattttcg
     7321 aagagtat
//
LOCUS       DAZQCJ010000095         7309 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000095 DAZQCJ010000000
VERSION     DAZQCJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.096"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(110..1450)
                     /locus_tag="ACHU66_001762"
     CDS             complement(110..1450)
                     /locus_tag="ACHU66_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HFR3390028.1"
                     /translation="MRTSFIQSSHQEACIQAIQELVAFPSVLQEHQADTPFGQAIQDV
                     LEHTLALTEKMGFKTYLDPAGYYGYAEIGQGEELLAILCHLDVVPAGDLSQWQTPPFE
                     AVVEGDYIIGRGVQDDKGPSMAALFAVKALLDAGVQFNKRIRFIFGTDEETLWRCMNR
                     YNQLEEVATMGFAPDSSFPLTYAEKGLLQAKLHGPGHPCLSIEAGTAYNVVPAKASYS
                     GHLLAGVIAELDQLGFDYETKDDQVTVLGISRHAKDAAEGVNAIVRLAKALEHFESHP
                     ALDFIVNAVGEDATGFKLFGDVTDEPSGTLSFNIAGLTISAKKSEIRLDIRIPVTADK
                     EALVSTLQAKAQSCGLTYEEYDYLASLYVPLDSQLVSTLMSVYQDKTGDLTSEPISSG
                     GATFARTMPNCVAFGACFPDTEQTEHQENERMPLEDLYKTMDIYAEAVYRLAAE"
     gene            complement(1569..3071)
                     /locus_tag="ACHU66_001763"
     CDS             complement(1569..3071)
                     /locus_tag="ACHU66_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFR3390029.1"
                     /translation="MSEKVKKGFKLPSSYTILMLIIAFMAVMTWIIPAGQYQVDEAGR
                     LVAGTYEKVAQNPQGIYDVFMAPVRAMLGHGATEAAINVAFFIIMVGAFLGVVNETGA
                     LDVGIASIVKRFKGREKMLIYILMPLFALGGSTYGMGEETMAFFPLLVPVMMAVGFDS
                     ITGVAIILLGSQIGCLASTVNPFATVVASDAAGVSVADGMIWRLVFFVVILLMGVLFV
                     ANYAEKVKNDPTKSLVYKQREADMQHFNVTATQEVNAELSPAQKRVLWVFVLTFVLMI
                     CSFIPWEDLGVTIFTQFKDWLIGLPFIGQVIGSSTAALGTWYFPEGAMLFGIAGIVVG
                     LVYGMSEERLVKSFMFGAADIFSVALICAIARGIQVIMNDGMMTATILHMGEEGLQGL
                     SSQVFIILTYLFYLPMSFLIPSSSGLAGASMGIMAPLGEFVNVPASLVITAFQAASGL
                     LNLVAPTSGIVIGALALGRVEIGTWYKFVGKLIVGIMIASIAILVIATFF"
     gene            complement(3414..4361)
                     /gene="arcC"
                     /locus_tag="ACHU66_001764"
     CDS             complement(3414..4361)
                     /gene="arcC"
                     /locus_tag="ACHU66_001764"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR3390030.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQKEALVQTAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQNLAADSEKNPAFPLDSLVAMTEGSIGFWLQNALENELHKE
                     GIEKDVASVVTQVIVDKNDPAFENLTKPIGPFYTEEEAKAEAEKTGATFKEDAGRGWR
                     KVVASPKPVGIKEIGTIRTLLNAGEVVVAAGGGGIPVVQEADGTLTGVEAVIDKDFAS
                     QCLAELVDADLFIVLTGVDYVFVNYNKPDQEKLETVTVAELEEYIKQNQFAPGSMLPK
                     VEAAIAFVNNKPQSKAVITSLENLGALIESDSGTIIVKG"
     gene            complement(4479..5492)
                     /gene="argF"
                     /locus_tag="ACHU66_001765"
     CDS             complement(4479..5492)
                     /gene="argF"
                     /locus_tag="ACHU66_001765"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR3390031.1"
                     /translation="MTNVFKGRHFLAEKDFTRAELEWLIDFSAHLKDLKKRNIPHRYL
                     EGKNIALLFEKTSTRTRAAFTVASIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQKMVEELAEFSGVPVWNGLTDAWHPTQMLADYLTVKENFGKLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEEEVVALAEVFAKESGARVLITDNA
                     DEAVKGADVLYTDVWVSMGEEDKFAERVALLKPYQVNMELVKKAENENLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGDPFV
                     PRV"
     gene            complement(5511..5951)
                     /locus_tag="ACHU66_001766"
     CDS             complement(5511..5951)
                     /locus_tag="ACHU66_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR3390032.1"
                     /translation="MPSIERTTIGSEEWQRAASIYVRYKVFVLERGIAKEGEFDQNDT
                     EGRVYANLFIGEEPVSTGRFLPVGPGQARLTRIATLPNFRGKGYGKMIISSLEAYARE
                     AGFHQLNIHSELTAKTFYESVGYQATSEVYQEDGEWCQTLTKFI"
     gene            complement(5951..7183)
                     /gene="arcA"
                     /locus_tag="ACHU66_001767"
     CDS             complement(5951..7183)
                     /gene="arcA"
                     /locus_tag="ACHU66_001767"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFR3390033.1"
                     /translation="MMSNHPIHVFSEIGKLKKVMLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRDEGVEVLYLEKLAAESLVTPEIREQFIDEYLEEANIRGRA
                     TKKAIRKLLLSIKDNQELVEKTMAGIQKAELPEIPAEEKGLTDLVESSYPFAIDPMPN
                     LYFTRDPFATIGNAVSLNHMYSETRNRETLYGKYIFTHHPEYGGKVPLVYNREETTRI
                     EGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFEQHVGFKKVLAFEFANNRKFMHLD
                     TVFTMVDYDKFTIHPEIEGDLRVFSVTYENDTLHIEEEHGDLAELLAANLGLEKVELI
                     RCGGGDMVAAGREQWNDGSNTLTIAPGVVVVYKRNTITNAILESKGLRLIKIGGSELV
                     RGRGGPRCMSMPFEREDI"
BASE COUNT         2171 a         1689 c         1364 g         2085 t
ORIGIN      
        1 gtgacacatt tctatctcat ctttctaatg atattcttca taagaaaagc ccttagatat
       61 ttcactaaga gctttgaaac tgtaatttac tattcaatac tgataatggc tattccgcag
      121 ctaggcgata gactgcctca gcataaatat ccattgtttt atataagtct tccaatggca
      181 tgcgttcatt ttcctgatgc tctgtttgtt ctgtatctgg gaaacatgca ccaaaggcca
      241 cacagtttgg catagtacga gcaaaggtag ccccacctga cgagattggc tcgcttgtta
      301 aatctcctgt cttatcttga taaacagaca tgagcgtaga taccagctgg ctgtctaatg
      361 gcacatacaa ggaagcaagg tagtcgtact cttcataggt caagccacat gattgcgctt
      421 tggcttgtaa agtagaaact agggcttctt tatccgcagt gacaggaata cgaatgtcca
      481 agcgaatttc tgattttttt gcgctaatgg ttagacctgc aatattgaaa ctgagtgtcc
      541 ctgatggttc atcggtcaca tcaccaaaaa gcttaaatcc tgtggcatct tcaccaactg
      601 cattgacgat aaagtccaag gctggatgac tttcgaaatg ttccaaagcc tttgccaaac
      661 gaacgatggc attgaccccc tcggcagcat ccttggcgtg ccgcgaaata ccaagaaccg
      721 ttacttgatc atctttggtt tcataatcaa agcctaattg atctaattcc gcaataactc
      781 cggctaataa atgaccagaa tagcttgcct tggcaggtac tacgttatag gcagttcccg
      841 cttcgatact cagacaaggg tgacctggac catgcaattt ggcctgtagc aatccttttt
      901 cagcataagt caaagggaag gaagaatctg gtgcaaagcc catagttgct acttcttcca
      961 actgattgta gcggttcata caccgccata aggtctcttc atctgttcca aaaatgaaac
     1021 gaatccgttt gttaaattgg actcccgcat caagaagtgc ttttactgca aataaggccg
     1081 ccatcgaagg acctttatca tcctgaaccc cgcgtccaat aatataatct ccctctacca
     1141 cagcctcaaa aggcggcgtc tgccattgac taagatcacc agcaggtacc acatctaagt
     1201 ggcaaaggat agctaataat tcttcgcctt gcccaatttc agcatagccg taataaccag
     1261 cagggtctaa ataagtttta aaacccattt tttccgtcaa agccaacgtg tgctccagaa
     1321 catcctgaat cgcttgacca aatggcgtat ctgcctgatg ttcttgcaaa acagaaggaa
     1381 aagcaaccag ttcttggatg gcttgaatac atgcctcctg atgcgatgac tgaataaaac
     1441 ttgttctcat tgtgtcccct ttcaacataa taagatatga tatattttct agcactattc
     1501 tatcataaaa ggaaaaactg agccaaattt tatctgtatt cgtgataaaa tctgacccag
     1561 aatttcgatt agaagaatgt cgcaatgacc aaaatagcta tggaggcaat catgatgcct
     1621 acgattaact tacctacaaa tttataccaa gtaccaattt caacacgacc aagtgccaag
     1681 gctcctatta caatacctga tgttggagcc accaagttca agagacctga tgcagcttgg
     1741 aaagctgtga taaccaagct tgctggtaca tttacaaact caccaagagg tgccatgata
     1801 cccatagaag cccctgcaag acctgatgaa gatggaatca agaaggacat tggtaggtag
     1861 aagagatagg tcaaaatgat gaatacttgt gaagataaac cttgcaatcc ttcttcaccc
     1921 atgtggagaa tagtggcagt catcatacca tcgttcatga tgacttgaat accacgcgcg
     1981 atagcacaaa tcaaagctac gctgaaaata tcagccgcac cgaacatgaa ggattttacc
     2041 aaacgttctt cactcatacc ataaactaaa ccaactacaa tccctgcgat accaaagagc
     2101 attgctcctt ctgggaagta ccaagtacca agagcagctg ttgaagaacc aatcacttga
     2161 ccgatgaacg gaagaccaat caaccaatct ttaaattgtg taaagatagt aacacccaaa
     2221 tcttcccatg gaataaagct acaaatcatg agaacaaatg ttaatacaaa tacccagaga
     2281 acacgttttt gagctggtga taattctgca ttcacttctt gagttgctgt tacattaaag
     2341 tgttgcatgt ccgcttcacg ttgtttataa accaaggact tagtcggatc gtttttcact
     2401 ttctcagcat agttggctac aaagagaacc cccatcaaga gaataacaac gaagaagacc
     2461 aagcgccaaa tcataccgtc cgcaacactt acacctgctg catcagaggc tacaaccgtt
     2521 gcaaatgggt ttacagtaga agccaaacag ccgatttgag aacctagcaa gatgatggct
     2581 acaccagtaa tactatcaaa accaaccgcc atcataacag gaacaaggag cgggaagaaa
     2641 gccattgttt cttcacccat accataggtt gagcctccta aggcaaacaa aggcatgagg
     2701 atgtaaatca acattttttc acgacctttg aagcgtttta caatagaagc aatccctaca
     2761 tcaagtgcac ctgtttcgtt aactacacca agaaaggcgc caaccatgat gatgaagaat
     2821 gctacgttaa tcgctgcttc agtcgcaccg tgaccaagca tggcacgaac tggagccata
     2881 aaaacatcgt aaatcccttg tggattttga gcaaccttct cataggtacc agccaccaaa
     2941 cgtccggctt catccacctg atattgacca gctggaataa tccatgtcat cactgccata
     3001 aaggcaataa tgagcatcaa tattgtataa gacgaaggca atttaaagcc ttttttcact
     3061 ttttcgctca tcgcaaaaac ctcctataat attttaaaag tttatcccct atttaaggga
     3121 aattgtatcc gtaataaccg tatcaaatac ccacctatct tcaagagatg tctatctcct
     3181 tcagtctatt atctgaaatc attgcttata gatagatact tgataggaaa tggatgagag
     3241 gggagggagc ttagaagccc agaccttcat gtttagatta aagggaaccc ttgtgggact
     3301 atcaacactt tcactcccct aactccatca ataaataacc cgtctagttt aaaaactata
     3361 tttgcatgca gagtacatca ataaatagaa gcctcaacga tacatacagc tgcttagcct
     3421 ttgacaatga tagtgccgct atcggattca atcaaagcac ctaagttttc taatgaggta
     3481 atgactgctt ttgattgcgg cttattgttt acaaaagcaa tagctgcttc aactttcggt
     3541 agcatggaac ctggtgcgaa ttgattttgt ttgatatatt cttccaattc agccacagta
     3601 acggtttcta atttttcttg gtctggtttg ttgtagttaa caaagacata gtccacacct
     3661 gtcaagacga taaacaagtc tgcatcaacc aattctgcca aacattgaga agcaaagtcc
     3721 ttatcgataa ctgcttccac accggtaaga gtaccgtctg cttcttggac aactggaata
     3781 cctccgcctc cagcagctac gactacctca cctgcattga gaagggtacg aatggtacca
     3841 atttctttga ttcctacagg ttttggcgaa gcaacaactt tgcgccagcc acgaccggca
     3901 tcttctttga aggttgcacc agtcttttct gcttctgctt tcgcttcttc ttctgtatag
     3961 aatggtccaa tcggcttggt caaattttcg aaggcaggat cattcttatc cacgataacc
     4021 tgggtcacaa cagatgctac atctttttca atgccttctt tgtgcaattc attttccaag
     4081 gcattttgca accagaaacc gatcgaacct tctgtcatgg ctaccaaact gtctagtgga
     4141 aaagctgggt tcttttccga atcagcagca aggttttgaa gaagtaagtt tccaacctga
     4201 ggaccattac catgcgtgat aataaggtcg tccccatttt ttatcaattt tacaaggtgc
     4261 ttggctgttt gtaccaaggc ctctttttgt gctttagccg atgggtcgga cgataaaatg
     4321 gcattgccac ccaaggctac tacaatttta cgatttgcca tatctcttgc tttttcctca
     4381 ctttcgcgaa atagtaatcg cttacacatc tatcctcaaa atatgggggc tgggtgccag
     4441 cccccggagg ttgcgatgcg ttattagatc gttaattatt acacacgtgg aacaaatgga
     4501 tcccctaaag tagccgccat cactgcttta atagtgtgca tacggttttc agcttggtcg
     4561 aagtgacgag catatttgct acggaatact tcgtctgtta cttccatttc ttctacgccg
     4621 aatttttcag cgacatcttt accataaaca gtgtttgtat catggaaggc aggcaagcag
     4681 tgcaagaaga tgagattttc attttctgct tttttcacca attccatatt cacttggtat
     4741 ggtttcaaga gggcaacgcg ttctgcaaat ttatcttctt cacccattga tacccaaacg
     4801 tcagtataaa gaacatctgc acctttgact gcttcgtcag cattatcagt aatgagaaca
     4861 cgtgctccac tttctttcgc aaaaacttca gccaaggcaa ctacttcttc ttctgggaag
     4921 agttcttttg gagagaagat atggacattg acaccaagga tagcacctgt caccaagaga
     4981 gagttggcaa cgttgttacg accatcacca cagtaaacca gagtcaaacc ttccaacttg
     5041 ccaaagtttt ctttgacagt caagtagtcc gccaacattt gagttgggtg ccatgcatct
     5101 gtcaaaccat tccatactgg cacaccagag aattctgcca attcttccac cattttttga
     5161 ctgaaaccac ggaattcaat accgtcgaac atacgcccca aaactttagc agtatcttct
     5221 gtagattctt tcttaccaag ttggatatca tttgcaccaa gatattctgg atgggcacca
     5281 aggtcaatag atgctactgt gaaggcagca cgagtacgtg ttgatgtttt ttcaaacaag
     5341 agggcaatat ttttaccttc caaataacgg tgtggaatat tgcgtttttt caaatctttc
     5401 aaatgagctg agaaatcaat caaccattcc aattctgcgc gtgtgaaatc tttctctgca
     5461 aggaaatgtc tacctttaaa tacgtttgtc attgtctctt ctcctaatgt ttaaataaat
     5521 tttgtcaatg tctggcacca ttctccatct tcttgataaa cttcagaagt tgcttggtag
     5581 ccaactgact cataaaaagt ctttgcagtt aactccgaat gtatattcaa ctgatgaaag
     5641 cctgcttcac gtgcataagc ttccaaagag ctgataatca tttttccata gccttttcca
     5701 cgaaaattcg gtagagtggc tattcgggtt agacgagctt gaccaggacc gacaggaaga
     5761 aaacgtcccg tcgaaacagg ctcttcacca ataaagagat tggcgtaaac tcttccttcc
     5821 gtatcattct ggtcaaattc accttccttt gcaataccac gctcaaggac aaatacctta
     5881 tagcggacat agatagaggc tgcgcgttgc cattcttccg aaccgattgt tgttcgttct
     5941 atacttggca ttagatgtct tcccgttcaa acggcattga catacaacga ggtccaccac
     6001 gaccgcgaac caattcgctt ccgccaatct taatcaaacg aagccctttg gactcgagga
     6061 tagcattggt aatcgtattg cgtttgtaaa caactaccac acctggtgca atagtcaatg
     6121 tatttgaacc atcattccat tgctcacgac cagcagcaac catatcaccg ccaccacaac
     6181 ggatcaattc tactttttca agaccaaggt tcgcagccaa aagttctgcc aaatcaccat
     6241 gttcttcttc aatatgaagc gtgtcatttt cataggttac agagaagaca cgaaggtcac
     6301 cttcgatttc tgggtggata gtaaacttat catagtcaac catggtaaat accgtatcca
     6361 aatgcatgaa tttacggttg ttagcaaatt cgaatgccaa tactttcttg aagccaacat
     6421 gctgttcaaa gatatttacc aatagttttt caattgatgc tgcgtcagta cgttgtgaaa
     6481 tacctactgc caaaacatct tttgaaagaa ccaattcatc cccaccctcg atacgagtag
     6541 tttcttcacg attgtaaacc aatggaactt ttccaccata ttctgggtga tgagtgaaga
     6601 tgtatttacc atacaaggtt tcacggttcc gtgtttctga atacatgtgg ttaagagaaa
     6661 cagcattacc aatcgtagca aatggatccc gtgtgaagta aaggtttggc attgggtcta
     6721 ttgcaaatgg gtaagaggat tcaaccaaat ctgtcaaacc tttttcttca gcagggattt
     6781 ctggcaattc tgccttttga ataccggcca tcgttttctc aaccagttct tgattatctt
     6841 taatagacaa caacaacttg cgaattgcct tcttagtcgc ccgaccacga atgttagctt
     6901 cttcgagata ttcatcaata aattgttcac gaatttcagg tgtaacaagt gactcagcag
     6961 ctaatttttc caagtagagc acttctacac cttcatcacg aagagcttga gcaaaggcat
     7021 catgctcttt ctgtgcatct tctaagaatg gaatatcatc gaagagtagg cgttctaggt
     7081 agtcaggcat aaggttttca atttccttac ctggtctgtg taacataact tttttcagct
     7141 taccaatttc tgaaaaaaca tgaattggat ggtttgacat gatctgtcct ccttgtagat
     7201 ttatacatga tgggaaacga ggaggaccgt acgctttcct gcctctcatt tcctctttac
     7261 atccatagtt taacagagta aaaaaacaaa aacttgaaaa cgctcacat
//
LOCUS       DAZQCJ010000096         7173 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000096 DAZQCJ010000000
VERSION     DAZQCJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.097"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            97..2049
                     /gene="gyrB"
                     /locus_tag="ACHU66_001768"
     CDS             97..2049
                     /gene="gyrB"
                     /locus_tag="ACHU66_001768"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR3390034.1"
                     /translation="MTEEIKDLQEKAQEYDASQIQVLEGLEAVRMRPGMYIGSTSKEG
                     LHHLVWEIVDNSIDEALAGFADKIEVYIEPDNSITVIDNGRGIPVDIQEKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVYKNGQVYFQEYKRGEVV
                     ADLEVVGETDRSGTTVHFTPDPEIFTETTEFDFAKLNKRIQELAFLNRGLNLSITDKR
                     EGVEQTKEYHYEGGIASYVEYLNENKDVIFETPIYTEGEMDDITVEVAMQYTTSYHEN
                     VVSFANNIHTHEGGTHEQGFRTALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPGPQFEGQTKTKLGNSEVVKITNRLFSDAFAEFLLENPQIARRIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNDATKTELFIVEGDSAGGSAKSGRDRE
                     FQAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGADFDVSKARYQKLVIMTD
                     ADVDGAHIRTLLLTLFYRYMRPVVEAGYVYIAQPPIYGIKVGSEIKEYIQPGVNQEQE
                     LQDALERHSTGRSKPTIQRYKGLGEMDDHQLWETTMNPENRLMARVSVDDAAEADKIF
                     DMLMGDKVEPRREFIEENAVYSTLDV"
     gene            2138..3862
                     /gene="ezrA"
                     /locus_tag="ACHU66_001769"
     CDS             2138..3862
                     /gene="ezrA"
                     /locus_tag="ACHU66_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFR3390035.1"
                     /translation="MPTGTIILIVSIVIILIIAYVACLIVRKRNDNLLVALEERKEEL
                     FNLPVNEEVEAVKALHLIGQSQVSFREWNQKWVDLSLNSFADIENHIFEAEGYNNAFR
                     FVSAKNAIDSIESQIDLIEEDIASIRHGLMELKEQEEKNSGRVKHALNLFDSLQEAVR
                     ENPDSYGETLPELEKQLKNIEVEFSEFVMLNSSGDPIEASEILDKTEEHMIALNQIMD
                     RIPSLIERVTKDFPEQLEDLESGYRKLVEQNYLFTEANIESQFQNIRVSIRENTALIV
                     SFDLDAAEAENEGIQAKIDHLYKVFNREIEANKEAVKISKNLPKFLEHVVQNTQLLDE
                     ESQRLNATYLLADSKLSRINQLKARLESIEIVVTESVEDIENPQVAYSILEERLDHSL
                     ASLKEIEEEQLVLADYLKSQELSENTARKKATLYINKLHTLKRYMEKRNLPGIPAEFL
                     TNFFRTSDHVEALIAELDYKRINIEVVNRLLENATYDMNQLEELAYLIVQNATLTEQL
                     LQYSNRYRSFDESVQKAFNRSLSIFEKDFDYQAAFEEISFALETVEPGVTERFVRSYE
                     KTREAIRY"
     gene            3914..4549
                     /gene="serB"
                     /locus_tag="ACHU66_001770"
     CDS             3914..4549
                     /gene="serB"
                     /locus_tag="ACHU66_001770"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFR3390036.1"
                     /translation="MTTGLLVMDVDSTLILEEGIDLLGEEAGLGAQVAAITERAMRGE
                     LDFEEALRERVALLKGLPVSVFDRIIKKIHFTPGAAELVSELKMRGYKVAVVSGGFHE
                     TVDRLAAQLELDYVRANRLEVVDGVLTGQVLGEIVTKDTKKACLEEWAAENGLSLSQT
                     IAMGDGANDLPMIQRAGIGVAFCAKPIVQEQAPYQINEKNLYKLIEILDSK"
     gene            complement(4580..5230)
                     /locus_tag="ACHU66_001771"
     CDS             complement(4580..5230)
                     /locus_tag="ACHU66_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFR3390037.1"
                     /translation="MYQTILFDLDGTLTDSGQGILNSVAYALEKMGIEEPDTANLNRF
                     IGPPLYESFSRFYQLNPEDTQSAVDTFRVYFKEKGMFENQLYPGIIPLLEELRTAGKT
                     LVIATSKPEIFAKQILEHFGIAHYFDIIAGASLDSSRISKADVIGYAINQLEAFPKHA
                     VMIGDREHDIEGARMHQLPAIGVLYGYGNKQEFEKAGATMIVETVQDLKRVLLTTE"
     gene            5338..6465
                     /locus_tag="ACHU66_001772"
     CDS             5338..6465
                     /locus_tag="ACHU66_001772"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFR3390038.1"
                     /translation="MHILIAPDSFKESLSATKVAEAIKKGFSKVYPQAGYDLIPLGDG
                     GEGTVESLMQALSLDGTQTWVTGPFGDKIKVSYAVKDDLAVFEMAEIVGLASIPHERR
                     SPLFIKTQGVGELIVELVQNGVKRIFIGVGGSASHDGGIGMAAGLGVKFYNREGKEVE
                     AIGAHIGEIVSFSTEDMLVDLSQVRIDLVTDVENPLCGPAGATFVFAGQKGLAPAEFE
                     LVDKKMESFYKLVNPMLLDLAGAGAGGGMAAGLVQFAGARIQKGIDFVLDQLDFAHRV
                     TKADLVVVGEGRMDAQSLSGKTPIGVARRTPTGIPIIAICGSLKDDLPEFPFENICAA
                     FPIIASVESLEDTLQKAEKNLVRTAEQVARILQLGGQQRWN"
     gene            6456..6683
                     /locus_tag="ACHU66_001773"
     CDS             6456..6683
                     /locus_tag="ACHU66_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390039.1"
                     /translation="MELVKDVAIILFIFICLAKLVSVMRLSEKERGMEMNVIKANGAM
                     FFFNEKNERGRLLGILCLTCIIFSLIFILLY"
     gene            complement(6693..7145)
                     /locus_tag="ACHU66_001774"
     CDS             complement(6693..7145)
                     /locus_tag="ACHU66_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1694 domain-containing protein"
                     /protein_id="HFR3390040.1"
                     /translation="MNDLNTTILQKASGETRLNPDEQRLYMGTYRERVVLIVSFDDLS
                     SEVVGNKFDTICQKLANRYSPLFLKLSPALSDKQQISLLKIAQNFGITTAIIDEKIGQ
                     SPYALVFHTNHAVDNEEVSLESIFPNLISKPEEKKEDAKPSFWKKLFG"
BASE COUNT         2187 a         1223 c         1677 g         2086 t
ORIGIN      
        1 agaatgattt ttgctctaaa aatatgtttc gcttttatcc agatggattg gcttttctat
       61 ttttaaggag caacaaatag aaaataagga aaagaaatga cagaagaaat caaagattta
      121 caagagaagg ctcaagaata tgatgccagt cagattcaag tcttggaagg tcttgaagct
      181 gttcgaatgc gtccagggat gtatatcggc tcaacatcta aagaaggtct ccaccacttg
      241 gtttgggaaa ttgtcgacaa ctcaatcgat gaggcgctgg ctggttttgc ggataaaatt
      301 gaagtttata ttgagccaga taactccatc acggttattg ataatggacg tggtattccg
      361 gtagatattc aggaaaaaac tggtcgtcca gcggtagaaa ctgtctttac agtgctccat
      421 gcaggtggta aatttggcgg aggcggttat aaggtctcag gtggattgca cggcgtaggt
      481 tcatcagtcg taaacgccct atctactcag ctagatgtcc atgtctacaa aaatggtcag
      541 gtatatttcc aagagtataa acgaggtgaa gtcgttgcag atttggaggt tgttggagaa
      601 acagaccgtt ctggtacaac ggtacatttt acacctgatc cagaaatttt tactgagacg
      661 actgagttcg actttgcaaa attaaataag cggatccaag aattggcctt cctaaatcgt
      721 ggtttaaatc tctctattac ggacaaacgt gagggagtag agcaaacaaa ggaatatcac
      781 tatgaaggtg gtattgcttc ctacgttgag tatcttaatg agaacaagga tgtcattttt
      841 gagaccccta tctatacaga aggtgaaatg gatgatatta ccgttgaggt tgccatgcaa
      901 tatacgacga gttatcatga aaacgtagtt agttttgcca acaatattca cacacatgaa
      961 ggtggtaccc atgagcaagg attccgtaca gctctgacac gtgttatcaa cgactacgcc
     1021 aagaaaaata aaattctcaa agaaaatgag gataatttga ctggtgaaga tgtgcgtgaa
     1081 ggcttgacag cggtcatttc tgttaagcac ccaggtcctc aatttgaggg acaaactaag
     1141 acaaaacttg ggaatagtga agtggtcaaa attaccaacc gccttttctc tgatgccttt
     1201 gcagaatttc ttttggaaaa tccacagatt gctagacgga ttgttgaaaa agggattttg
     1261 gcaagcaagg cgcgtattgc tgctaagcgt gcgcgtgagg taactcgtaa gaaatcgggt
     1321 cttgaaatct caaacctccc aggtaagtta gcagactgtt cctcaaatga tgcaacgaaa
     1381 acagagcttt tcatcgttga gggggactca gccggtggtt cagctaaatc cggtcgcgac
     1441 cgtgaatttc aggctatttt accgattcgt ggtaagattt tgaacgtaga aaaagccagc
     1501 atggacaaga ttttggccaa cgaagaaatc cgtagtctat ttacagctat gggaactggc
     1561 tttggtgcgg atttcgacgt tagcaaggca cgttatcaaa aattagtcat tatgacagat
     1621 gccgatgtcg atggtgccca tattcggaca ctccttctga cattatttta ccgctacatg
     1681 cgtcctgttg tagaagcggg atacgtatat atcgctcaac cacctattta cggtatcaag
     1741 gttggaagtg agataaaaga atatattcaa cctggggtta atcaagagca agaattgcaa
     1801 gatgccttag aaagacatag tacgggacgg tccaagccaa cgatccaacg ttacaaaggt
     1861 ttgggagaaa tggatgatca tcagttgtgg gaaactacga tgaatcctga aaatcgtctc
     1921 atggcacgcg tttcagttga tgatgcggca gaagccgata agatttttga catgttaatg
     1981 ggggataagg ttgaacctcg tcgagagttt atcgaggaaa atgctgttta ttcaacattg
     2041 gatgtctaaa aactaaaaaa acaagtgcat atactagaga tttatggtat aattaagcga
     2101 tattttctag taattactat gagctaagga gatttacatg cctacaggaa caatcatctt
     2161 aatcgtttcg attgttatta ttttaatcat tgcctatgta gcttgcctga ttgttcgtaa
     2221 acgaaatgac aaccttttgg ttgcattgga agaacgtaag gaagagctat ttaaccttcc
     2281 agttaatgaa gaagtcgaag ctgtcaaagc acttcatttg attggtcaaa gtcaggtgtc
     2341 attccgtgaa tggaatcaaa aatgggttga tttgtcgctc aattcatttg ccgacattga
     2401 aaatcatatc tttgaggcag aaggctataa caatgccttt cgctttgtga gtgcaaagaa
     2461 cgctatcgac agtatcgaaa gccaaattga tttaattgaa gaagacatcg cctctatccg
     2521 tcatggtttg atggagttga aggagcaaga agagaaaaac tctggtcgcg taaaacatgc
     2581 gttaaacttg ttcgatagtt tacaggaagc tgttcgtgaa aatccagata gctacggtga
     2641 gacactacca gaacttgaga aacaattgaa aaatattgaa gttgagtttt ctgaatttgt
     2701 gatgctcaat tcctctggtg acccgattga agcttctgaa atccttgata agactgaaga
     2761 gcatatgatt gccctcaatc aaatcatgga tcgcattcca agtttgattg aacgagttac
     2821 aaaagatttc ccagagcaat tggaagattt ggagtcgggt tatcgtaaac tggttgagca
     2881 gaattatttg ttcacagaag ctaacatcga gtcacagttc caaaatattc gcgtttcaat
     2941 tcgtgaaaat actgctttga ttgtttcctt tgatttggat gccgcagaag ctgaaaatga
     3001 gggaattcaa gcaaaaattg atcatttgta taaagtcttc aatcgtgaga tcgaagcgaa
     3061 taaggaagct gttaagatta gtaaaaactt gccaaaattc ttggagcatg ttgtccaaaa
     3121 tacacagctc ttggatgagg aaagccaacg cctaaatgca acttacttgc tggcagatag
     3181 caaactttca cggatcaatc agcttaaagc tcgtttggaa tcgattgaaa ttgttgttac
     3241 agaaagtgtg gaagacattg aaaatccaca agttgcatat tctattttgg aagagcgttt
     3301 ggaccattca ttggcaagct tgaaagaaat cgaagaggag cagcttgttt tagctgacta
     3361 tcttaaatca caagaattgt cagagaacac agcgcgtaag aaagctacac tttatatcaa
     3421 caaattgcat acccttaagc gttatatgga aaaacgcaac cttccaggaa ttccagctga
     3481 atttttaaca aacttcttta gaacgagcga tcatgtggaa gccttgattg ccgagttaga
     3541 ctataaacga atcaatatcg aagttgttaa tcgtttgttg gaaaatgcaa cgtatgatat
     3601 gaatcaattg gaagagttgg cgtatttgat tgtgcaaaat gcgactttaa cagagcagtt
     3661 gctacaatac tctaaccgtt atcgttcatt cgatgaaagt gtgcaaaaag catttaaccg
     3721 ttcattatca atttttgaaa aggattttga ttaccaagca gcatttgaag aaatttcatt
     3781 tgctctggaa acagttgaac ctggtgtaac agagcgtttt gtacgttcat acgaaaaaac
     3841 tcgcgaagcc attcgctact agataaaaag ccatttggct ttttattttt atactttgaa
     3901 aggaatattg gagatgacaa ctggattatt agttatggat gttgattcaa cattaatttt
     3961 ggaagaagga atagatttac ttggagaaga ggccggttta ggagcgcaag ttgcagcaat
     4021 taccgaacgt gctatgcgtg gggaattgga ttttgaagaa gccttacgtg aacgtgtggc
     4081 gttgttaaag ggactgcctg tttctgtgtt tgatcgaatt atcaaaaaaa tccactttac
     4141 gcctggagca gcagaattgg tctcagaatt aaaaatgcga ggctataagg tagcagtagt
     4201 ttcaggcggg tttcatgaaa cagtcgatcg actagctgca cagttagagt tagactatgt
     4261 tcgggctaat cgattagaag tagtggatgg agtattgacg ggacaagtat tgggtgaaat
     4321 tgtaacgaaa gatactaaaa aagcatgttt agaagaatgg gcggcagaaa atgggctgag
     4381 tctatctcaa acgattgcca tgggggatgg agcaaatgat ctaccaatga ttcaacgagc
     4441 aggaattgga gtagctttct gtgctaaacc gattgtccaa gaacaggcgc cctaccaaat
     4501 aaatgaaaaa aatctctata aactaataga gattttagat agtaaataac gagaagtcag
     4561 ctgagtgctg acttttcttc tattctgttg tcagtaaaac ccttttcaaa tcttggacgg
     4621 tttcgactat catggtagcc ccagcctttt caaattcctg cttgttccca tagccgtaaa
     4681 gaacaccgat ggcgggaagt tggtgcatgc gtgctccttc aatgtcatgt tccctatctc
     4741 caatcatcac agcatgtttt ggaaatgctt ctaattgatt tatagcatag ccgatgacgt
     4801 ccgccttact gatgcgacta ctatccagac tagcaccggc aatgatatca aagtagtgag
     4861 cgataccaaa atgttctaga atctgtttgg caaatatttc tggctttgag gtagcaatta
     4921 ctagtgtttt tccagcagtt ctcaattctt caagcagagg gatgattcca ggatagagtt
     4981 gattttcaaa cattcctttt tctttaaaat agacacggaa agtatcaact gcactttgtg
     5041 tatcttcagg gttgagttgg tagaatcgtg aaaaagattc atagagtggc gggccgatga
     5101 atcggtttaa attggctgta tctggttctt cgattcccat tttttctagt gcgtaggcga
     5161 cggagtttaa gattccttgt ccagaatcag taagtgttcc atctaagtca aataatatag
     5221 tttgatacat acttatctcc tttctttcag tataacacag acgatgaaaa acagaaaaga
     5281 tttattgaaa gctgaatagg agcgtgatat actaataata taaaggaggc tagtaccatg
     5341 catattctca ttgctccgga ttcatttaaa gagtctcttt cagctacaaa ggttgctgaa
     5401 gcgattaaaa aaggattttc taaggtttac ccacaagcag ggtatgactt aattccccta
     5461 ggagatggtg gcgaagggac agttgagagt ctaatgcagg ccttatcctt ggatgggacc
     5521 cagacttggg tgacaggtcc atttggagat aaaattaagg tttcctatgc ggttaaagat
     5581 gatcttgcgg tttttgaaat ggctgagatt gtagggctgg caagcatccc gcatgaaaga
     5641 aggagtcctc tctttattaa gacacaaggg gttggggagc tgattgtgga gctcgttcaa
     5701 aatggtgtca aacgcatatt tataggagta ggtggctctg ccagtcatga tggtggtatc
     5761 ggaatggcgg caggtttagg agtgaaattt tataatcgag agggaaaaga agtagaggca
     5821 attggagctc atatcgggga gattgtcagc ttttccacag aggatatgtt ggtagatcta
     5881 tctcaggtca ggattgattt ggtaacggat gtggagaatc cgctttgcgg accagcgggt
     5941 gccacatttg tttttgcagg tcagaaaggt cttgcccctg ctgaatttga actagtagac
     6001 aagaaaatgg agtcctttta taaattagtt aatccaatgc tactcgattt ggcaggagcg
     6061 ggtgctggag gaggtatggc agcaggttta gtccaatttg ctggtgctcg cattcaaaaa
     6121 ggcatcgact ttgtcttgga tcagttggac tttgctcatc gcgtcaccaa agcggatctg
     6181 gttgtggtcg gagaagggcg gatggatgcc caaagcttat cagggaagac tccgatagga
     6241 gtagctagac ggacgccgac ggggattccc atcattgcaa tttgtggtag cctcaaagac
     6301 gatcttcccg agtttccttt tgaaaatatc tgtgccgctt ttccgataat tgcaagcgtt
     6361 gaaagtttgg aggacacgct tcaaaaagca gaaaaaaacc tggtccgcac ggcagagcag
     6421 gtggcaagaa ttcttcagtt aggaggtcaa caaagatgga attagtgaaa gatgtggcta
     6481 ttatcttatt tatttttatt tgtcttgcaa agctagtatc cgttatgaga ctttccgaaa
     6541 aagaaagagg aatggaaatg aatgtaataa aggcaaatgg agcaatgttc tttttcaatg
     6601 aaaaaaatga aagaggcaga ttgctaggaa tcctttgtct aacctgtatt atttttagtt
     6661 taatatttat actgctttat tgataaataa gtctaaccaa ataatttttt ccagaaggaa
     6721 ggttttgcat cttccttttt ttcttctggt tttgagataa gatttggaaa tatggattct
     6781 aagctaactt cttcattatc cacagcgtga tttgtgtgaa agacaagtgc gtagggagac
     6841 tgtcctattt tttcatcgat gatagcggta gtaataccga agttctgagc aatttttagt
     6901 agtgaaattt gttgcttgtc agacagggct ggcgagagtt tgaggaatag aggagagtat
     6961 ctgtttgcta gtttttgaca aatcgtatcg aacttatttc cgacgacttc actactcaaa
     7021 tcgtcaaagg aaacgatcag gacaacccgt tcgcgatagg ttcccatgta tagacgttgt
     7081 tcatctggat tcaatcgtgt ttcgccagag gctttctgta gtatggtagt atttaaatcg
     7141 ttcatggttt cagtataaca tactcagtta act
//
LOCUS       DAZQCJ010000097         7032 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000097 DAZQCJ010000000
VERSION     DAZQCJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.098"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..801)
                     /locus_tag="ACHU66_001775"
     CDS             complement(<1..801)
                     /locus_tag="ACHU66_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFR3390041.1"
                     /translation="MNNQIILLTIQVFIVALILWMLYTILSYAKKHDISLKEKFWTGF
                     GIGYVTDLLDTLGIGTFATTTTMFKLTKLVEDDRKIPATMTTAHIIPVLVEALLFITI
                     VEVDMVTLIAMAAAAFTGAFVGAKVTQKWDTKKVQRILGILLIIAACFMVYRMITNPG
                     ADLTNEVKGLTGWKLAVGIAFDFMVGMLMSMGLGNYAPELIFFSLMGISPAVALPVMM
                     LNAAMIMTAGAKQFIQSGRVNWPGVPGIVVGGVLGVLTAAFFLSNLDIN"
     gene            900..2213
                     /locus_tag="ACHU66_001776"
     CDS             900..2213
                     /locus_tag="ACHU66_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFR3390042.1"
                     /translation="MIEKVFRDPVHNYVHVDHELIYKLINTKEFQRLRRIKQLGTTSY
                     TFHGGEHSRFSHCLGAYEIARRITQKFEDKYPQIWDTNESLLTMVAALLHDVGHGAYS
                     HTFERLFDTDHEEMTCAIITSPETEINALLKQVSPEFPDKVASVIKHTYPNKQVVQLI
                     SSQIDVDRMDYLLRDSYFTGANYGEFDLTRILRVIRPTENGIAFKESGMHAVEDYVLS
                     RYQMYMQVYFHPASRSMEVLLQNLLNRAKLLYTSEQEFFARTSPRLLPFFEHRIRLTD
                     YLSLDDGVMNTYFQSWIDGPDRILSDLAQRYINRKVLKSITFKAEEEEALDRLRSLVA
                     DVGFDPEYYTAIHHNFDLPYDIYRPNAEKKRTQIEIYRKDETLVELSSLSPIVHSLSG
                     TIHGDSRFYFPKEMLEETGIFAPQIADFNSHIHNDHFIIGESNEH"
     gene            2203..3012
                     /gene="yidA"
                     /locus_tag="ACHU66_001777"
     CDS             2203..3012
                     /gene="yidA"
                     /locus_tag="ACHU66_001777"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR3390043.1"
                     /translation="MSIKLVAIDIDGTLLNSQHQITAEVHSAIQESKKAGVKIVIATG
                     RPISGVQAILEELNLTEAGDYVITFNGGLVQDAATGEDIVKDTLTYEDYLDIESAARK
                     LKLPMHASTKEGIYTANRNIGKYTIYESTLVSAPIFYRTPEEMADKEIIKTMMVDEPE
                     ILDAAIPLLPTSLTEKYNVAKSAPFYLEITPKTVNKGQAIIHLAEKLGLTMDQTMAIG
                     DQENDRPMLEVVGNPVVMENGNPELKKIAKYITKSNDESGVAHALREWVLK"
     gene            complement(3066..3818)
                     /gene="tpiA"
                     /locus_tag="ACHU66_001778"
     CDS             complement(3066..3818)
                     /gene="tpiA"
                     /locus_tag="ACHU66_001778"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR3390044.1"
                     /translation="MSRKPIIAGNWKMNKNPQEAQAFVEAIAGKLPAGDKIEAAIAAP
                     AVDLNALLWFAKDSELKVAAQNCYFEDAGAFTGETSPKVLAEMGVNYVVIGHSERRDY
                     FHETDEDINKKAHAIFRNGLTPIICCGESLETYEAGKAVEFVGAQVSAALKDLTAEQV
                     ASLVIAYEPIWAIGTGKSATKDDAQNMCKAVRDVVAADFGQEVADKVRVQYGGSVNPS
                     NVAEYMACPDVDGALVGGASLEAESFLALLNF"
     gene            complement(3993..5189)
                     /gene="tuf"
                     /locus_tag="ACHU66_001779"
     CDS             complement(3993..5189)
                     /gene="tuf"
                     /locus_tag="ACHU66_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR3390045.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEIEIVGLQEEKSKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVISKPGSINPHTKFKGEVYILTKEEGGRHTPFFDNYRPQ
                     FYFRTTDVTGSIKLPEGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(5545..6516)
                     /locus_tag="ACHU66_001780"
     CDS             complement(5545..6516)
                     /locus_tag="ACHU66_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti sigma factor C-terminal domain-containing
                     protein"
                     /protein_id="HFR3390046.1"
                     /translation="MTMKTFEEVTKKSKRKNLWKTIGLSSLIGFILMLIGVQTVKIIV
                     GAREGRENWKQIILMEISYPNIKHDGIKIQSTSYVSGKLYCNLYKDLAGVPMAFGQHE
                     IYYDLFDRHYDFAQHIPGNTFKYLYDFTTNTKVPVFYNRKKSVWSAQDIANELHYLKD
                     MSGQLVEVAISFDKKYSLAEIKQMIPSNLKQNWYWIGTEGKQNTHYYRPEQLLGLDPE
                     DIQLVFSDMPGDYMTGMDFMKEFLESGDQERAQYLDIPVDDLQAFVDKFGGTDFTKQE
                     EIDQLEFAGIILTGKAEDFAQLEGKDWIYSSSIGASIPIQPYYQLHH"
     gene            complement(6497..7000)
                     /locus_tag="ACHU66_001781"
     CDS             complement(6497..7000)
                     /locus_tag="ACHU66_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFR3390047.1"
                     /translation="MSIKLDQYEKEIIGIAEEISYYLQKSGAGPADSQDIAQDVLVKL
                     LESDIVLPFEKLRAWLYRSAVRAYIDKYRRDKRYHEILQKEFFRREDLLVYDQEGYEE
                     LYQAVADLPEKYQQVIDLYYFQDMSVKEVAHILGTGQSWVKIILYRGRKQLRKILEER
                     GYDYEDF"
BASE COUNT         2104 a         1563 c         1307 g         2058 t
ORIGIN      
        1 attaatatcc aaatttgata ggaagaaagc ggctgtcaaa acacctagca ccccaccgac
       61 aacgattcct ggaactcctg gccagtttac tcgtcctgat tggataaact gcttggcacc
      121 agctgtcata atcatagcag cattcaacat cataactgga agagcaaccg ctggactaat
      181 tcccatcaat gagaagaaaa ttaactcagg agcatagtta cccagtccca tactcatcag
      241 cattcccacc ataaaatcaa aggcaatacc aacagccagt ttccaaccag ttaagccctt
      301 tacttcattg gtaagatctg cgccagggtt agtaatcatg cgatagacca tgaaacaggc
      361 tgcaataatt agtaaaatcc caagaattcg ttgaactttc tttgtatccc atttttgagt
      421 taccttggca ccaacaaagg cacccgtaaa tgccgctgct gccatagcaa tcagagtgac
      481 catatcaacc tcaactatcg taataaacaa tagcgcctct actaagactg gtatgatgtg
      541 cgccgttgtc atggtcgcag gaattttacg atcgtcttcc accaactttg tcagtttaaa
      601 catagtggtt gttgttgcaa aggttccaat ccccaaggtg tctagcaaat cagtaacata
      661 accgatacca aatcctgtcc aaaatttctc ttttagagaa atatcatgct ttttagcata
      721 ggatagaatt gtgtaaagca tccaaagaat cagggctaca ataaagacct gaatagttaa
      781 taagataatt tgattattca tttctccatt ataccaaaag attccattgg gtacatagca
      841 atatccttgt gttttagaaa aatcaggaat ttatctccct tttttgttat aatgaaacta
      901 tgatagaaaa agtatttcgc gatccagttc ataactatgt acatgttgat catgagctga
      961 tttataaact tatcaataca aaagaatttc agcgactacg ccgcatcaag caactaggga
     1021 ctacttccta taccttccat ggtggcgagc atagccgctt ttcgcactgc ctcggcgcct
     1081 acgaaatcgc cagacggatt actcaaaaat ttgaagacaa gtatccacaa atctgggata
     1141 caaatgagtc cctactgacc atggttgctg cgctactcca tgatgtcgga cacggagcct
     1201 attctcatac cttcgagcgt ctatttgata cagaccatga ggaaatgacc tgtgcaatta
     1261 ttactagtcc agaaacggaa atcaatgctc tgttgaaaca agtttcacct gagtttccag
     1321 acaaggttgc tagtgttatc aaacacacct atccaaataa acaggtcgtt caactcattt
     1381 cgagccagat tgatgttgac cggatggatt atctattacg agattcctac ttcacaggtg
     1441 cgaactatgg tgaatttgac ctaactagga tattacgtgt cattcgcccg acagaaaatg
     1501 gaattgcctt caaagaatct ggtatgcatg cagtagaaga ctacgttctc agccgctacc
     1561 agatgtacat gcaagtctat tttcatccag caagccgctc tatggaagtc ctattgcaga
     1621 atttactcaa tcgtgccaaa ctactatata ctagtgaaca ggagtttttt gcacgaactt
     1681 cacctcggct attacctttt tttgagcatc gtatccgtct aaccgactac ctcagtcttg
     1741 atgatggagt tatgaatact tactttcaat catggataga tggaccagat cgtatattat
     1801 ctgacttagc ccagcgctat atcaatagga aagtgctcaa atccatcact ttcaaggccg
     1861 aagaagagga agctttggac cgcctgcgta gcctggtcgc agacgtcggg tttgacccag
     1921 aatactatac tgctattcac cacaactttg atttgccgta tgacatttac cgaccgaatg
     1981 cagaaaagaa acggactcaa attgagattt atcgcaaaga tgaaaccttg gtagaactat
     2041 cctctctgtc accaattgtc cattccttat caggcacaat ccatggcgat agccgcttct
     2101 atttcccaaa agaaatgtta gaagaaacag gaattttcgc acctcaaata gctgatttta
     2161 acagccacat acataacgat cattttataa ttggagaatc aaatgagcat taaacttgtc
     2221 gccatcgata ttgatggtac actactaaat agtcaacacc aaattacagc tgaagtacac
     2281 tcagcaatcc aagaatcaaa aaaagcaggc gttaaaattg ttattgctac tggtcgcccc
     2341 atttccggtg ttcaagcaat tttagaagaa ctcaatctta cagaagcagg tgactacgtc
     2401 atcactttta acggtggctt agtccaagat gcagcaactg gtgaggatat tgtcaaagat
     2461 accttgactt atgaggacta tctcgacatc gaatctgctg cccgtaaact caagctccca
     2521 atgcatgcca gcaccaagga aggcatctat actgctaacc gtaacatcgg taaatacacc
     2581 atttacgaat caacactagt aagtgcaccc attttctatc gcactcctga agaaatggct
     2641 gacaaggaaa tcatcaaaac aatgatggtt gatgagccag aaattcttga tgctgctatt
     2701 ccacttctac cgaccagctt gactgagaag tacaacgtag caaaatcagc accattttat
     2761 ttggaaatca cccctaaaac tgttaacaag ggacaagcta tcattcatct agctgagaag
     2821 cttggactaa ctatggatca aactatggcc atcggtgacc aagaaaatga ccgtccaatg
     2881 ttggaagttg ttggtaatcc tgttgtcatg gaaaacggaa atcctgaatt gaagaaaatc
     2941 gccaaataca ttacgaagtc aaatgatgag agcggtgtag cccacgctct tagagaatgg
     3001 gtactgaaat agcctcttaa aatatcaaaa aaagcatcga attcaaaatc cgatgctttt
     3061 tgttattaga agttcaacaa agccaagaag ctttctgctt caagtgatgc accaccaaca
     3121 agcgcaccgt caacgtctgg acatgccatg tattcagcaa cgtttgatgg gtttacagaa
     3181 ccaccgtatt gaacgcgaac tttgtctgca acttcttgac cgaaatcagc agcaacaacg
     3241 tcacggacag ctttacacat gttttgtgca tcatctttag tagctgattt acctgtaccg
     3301 attgcccaga ttggctcata agcaataaca agtgatgcta cttgttcagc agtcaagtct
     3361 ttcaaagctg cagaaacttg tgcacctaca aattctactg ctttaccagc ttcgtaagtt
     3421 tcaagtgact caccacaaca gatgattgga gtcaaaccgt tacggaagat tgcatgagct
     3481 tttttattaa tatcttcatc agtttcgtgg aagtaatcac gacgctctga gtgaccgata
     3541 acaacatagt taacacccat ttcagccaat acttttggag atgtttcacc agtgaaggca
     3601 ccagcatctt caaagtagca gttttgagca gcaactttca attcagaatc tttagcgaac
     3661 cacaagagag cgttcaaatc tacagctgga gctgcgatag ctgcttcaat tttgtcgcct
     3721 gcaggcaatt ttcctgcaat tgcctcaacg aaagcttgtg cttcttgagg gtttttattc
     3781 attttccagt taccggcaat gattggttta cgtgacattt cacttacctc ttctatattt
     3841 attgtacaag gtacattata gcataaactt agctaagatt aaagaatttc tgtgcatttt
     3901 atgaatactt acagaaaaac tctctacccg aaagtagaga gttacaatta agttgtctga
     3961 ctataattgt tatttctggg aactcaatcg aattaagctt cgatttctgt aaccatacct
     4021 gaaccaacag tacgtccacc ttcacggata gagaaagtag taccttgttc aacggcgatt
     4081 gggtggatca attcaacgtc gatagtaacg ttatcaccag gcattaccat ttcagtacct
     4141 tctggcaatt tgattgaacc agttacgtca gttgtacgga agtagaactg tggacggtag
     4201 ttgtcgaaga atggagtgtg acgtccacct tcttctttag taaggatgta aacttcacct
     4261 ttgaatttag tgtgtgggtt gatagaacct ggtttagaga taacttgacc acgttcgatt
     4321 tcatcacgtt gtacaccacg aagaagcaca ccaacgttat cgccggcaag accttcgtca
     4381 agttgtttac ggaacatttc aacaccagta acaactgctt tagatttttc ttcttgaaga
     4441 ccaacgattt cgatttcgtc gttgacacga acagtaccac ggtcgatacg tcctgaagct
     4501 acagtaccac gaccagtgat tgagaatacg tcctcgactg gaagcaacaa tggtttgtca
     4561 gtgtcgcgtt ctggttctgg aatgtactca tcaacagtgt tcatcaattc cataacgatg
     4621 tcttcgtact tagagtcacc ttcaagagct ttaagagctg aaccttggat aactggaaga
     4681 tcatcacctg ggaaatcgta ttctgaaaga aggtcacgga tttccatttc aaccaactca
     4741 agcaattctt catcgtcaac caagtcaact ttgttcatga agacgataag gtgtttaaca
     4801 ccaacctgac gtgaaagaag gatgtgctca cgagtttgtg gcattggacc gtcagttgaa
     4861 gctactacaa ggatcgcacc gtccatctga gcagcaccag tgatcatgtt tttaacgtag
     4921 tccgcgtgtc ctggagcgtc gatgtgagca tagtgacgtt tttcagtttc gtactcaacg
     4981 tgtgcagtgt tgatagtgat accgcgctcg cgctcttctg gagcagcatc gatagacgca
     5041 tagtctttag gttggttaac tgatgaaggc aagcgacgtg ccaatacagt tgtgatagct
     5101 gctgtcaaag tagttttacc gtggtcaacg tgtccaattg taccaatgtt aacgtgtggt
     5161 ttactacgat cgtatttttc ttttgccatt ttggtaaaag cctccaataa aatatatttt
     5221 atagatagac agtaggcaat acagtctaac tttaccttac tattctatca aattgtagca
     5281 gaattgcaag cattttctat cgttttttat tttatccatt ttctgcaagg gctattagct
     5341 tcgcttataa ctttttttat catcccattt taacctatta ctcaactaaa aacacctcac
     5401 tgccatagga atattgatgt taccatagct acaaggtgcc acaaatacga gactactgcg
     5461 acagaccttt ctccattcag tcatcctacg actccctgac aagcctcttg ctataggtat
     5521 tgaaaatata ttgactattt tcacttaatg atgcagttga taataaggct gaataggaat
     5581 ggacgcgcca atactagaag aataaatcca gtccttgcct tccaactgag caaagtcttc
     5641 tgcttttcct gttaggataa tgcctgcgaa ctccaactga tcaatctctt cttgtttggt
     5701 aaagtcagtg ccaccaaact tatcgacaaa ggcctgcaag tcatccacag gtatgtccag
     5761 atactgtgct cgctcctgat ctcccgattc taagaactct ttcataaagt ccatacctgt
     5821 catatagtct ccaggcatat ccgaaaagac tagttgaata tcctctggat ctaaccccaa
     5881 aagctgctct ggacgatagt aatgggtatt ctgcttccct tctgtcccta tccaatacca
     5941 attttgcttg agattgctgg gaatcatttg cttgatttct gccaaactgt acttcttatc
     6001 aaaggaaata gccacttcta ctagctgccc actcatgtct ttaagataat gcaattcatt
     6061 ggcaatgtct tgagcactcc aaacagactt ttttctattg taaaaaacag gtaccttggt
     6121 attggttgtg aaatcatata gatatttgaa ggtattccca ggtatatgct gagcaaaatc
     6181 atagtgacga tcaaacaggt catagtaaat ttcgtgctga ccaaaagcca taggaacacc
     6241 agctagatcc ttatataaat tgcagtaaag tttcccagaa acataactcg tgctttggat
     6301 tttaataccg tcatgtttta tatttggata agagatttcc atcaaaataa tttgtttcca
     6361 attctcacga ccttctcgtg caccgacgat gatttttact gtttgaacgc caataagcat
     6421 taagatgaag ccgatgagcg atgatagccc tattgtcttc cataaatttt tccgcttact
     6481 cttttttgtc acctcttcaa aagtcttcat agtcatatcc tctctcttct agtattttcc
     6541 tcaactgctt gcgtccgcgg taaaggataa ttttcaccca actttgacca gtccctaaga
     6601 tatgggcaac ttctttgaca ctcatatctt gaaaataata gaggtcaatc acttgctggt
     6661 atttttctgg caggtctgca actgcttgat agagttcctc ataaccttct tggtcataga
     6721 ccaaaaggtc ttctcttcta aaaaactcct tttgtaaaat ttcatggtag cgcttgtctc
     6781 gcctgtattt gtcaatgtaa gcccgaactg cagatcggta aagccaagct cttagttttt
     6841 cgaaaggaag gacaatatca gattcaagca atttgactag aacatcttgg gcaatatcct
     6901 gactgtcagc tgggccggct cctgattttt gcaaatagta ggaaatctcc tctgcaatac
     6961 caataatttc tttttcatat tgatctagtt tgatacttat ctcctccttt cactgataaa
     7021 acgaataagg gg
//
LOCUS       DAZQCJ010000098         6859 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000098 DAZQCJ010000000
VERSION     DAZQCJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.099"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            64..246
                     /locus_tag="ACHU66_001782"
     CDS             64..246
                     /locus_tag="ACHU66_001782"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HFR3390048.1"
                     /translation="MPFVRIDLFEGRTEEQKIALAREVTEVVSRNTNAPKEAIHVFIN
                     DMPEGTYYPQGEMKRK"
     gene            complement(296..2128)
                     /gene="lepA"
                     /locus_tag="ACHU66_001783"
     CDS             complement(296..2128)
                     /gene="lepA"
                     /locus_tag="ACHU66_001783"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR3390049.1"
                     /translation="MNLEDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPIINKIDLPAADPERVRQEIEDVI
                     GLDASEAVPTSAKAGIGIEEILEQIVEKVPAPTGDVEAPLQALIFDSVYDPYRGVILQ
                     VRIVNGVVKPGDTIQMMSNGKTFDVTEVGIFTPKAIGRDYLATGDVGYIAASIKTVAD
                     TRVGDTVTLAKNPASEPLAGYKQMNPMVFAGIYPIDSNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERIEREFNIDLIMTAPSVVYHVNMTDGEMID
                     VANPSEFPDPTKIATIEEPYVKAQIMVPQEYVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYEISEYRSSKLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKIIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDEDDKK"
     gene            complement(2224..2637)
                     /gene="ndk"
                     /locus_tag="ACHU66_001784"
     CDS             complement(2224..2637)
                     /gene="ndk"
                     /locus_tag="ACHU66_001784"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR3390050.1"
                     /translation="MERTFFIIKPDALKRGLAGQILSRIERRGFQIRDLKMVTATEEL
                     ISQHYDHLTDKPFFPQLVQYMTSGPMIAGIIEGPEVIKSWRDMMGATNPVNALPGTIR
                     GDFATAPVEGIVANVVHGSDSAEAAEREIGLWLGK"
     gene            complement(2660..3247)
                     /gene="yihA"
                     /locus_tag="ACHU66_001785"
     CDS             complement(2660..3247)
                     /gene="yihA"
                     /locus_tag="ACHU66_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR3390051.1"
                     /translation="MEINTHNAEILLSAVSKAQYPGDDIPEIALAGRSNVGKSSFINT
                     LLGRKNLARTSGKPGKTQQLNFYNIDDKIRFVDVPGYGYAKVSKTERAKWGKMIEEYL
                     TTRENLRVVVSLVDMRHEPSADDVQMYDFLKYYEIPVIIVATKADKIPQGKWNKHESM
                     IKKKLDFDKNDQFVIFSSETRHGYDQAWDAILSNI"
     gene            complement(3303..4529)
                     /gene="clpX"
                     /locus_tag="ACHU66_001786"
     CDS             complement(3303..4529)
                     /gene="clpX"
                     /locus_tag="ACHU66_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFR3390052.1"
                     /translation="MAVKHTHELIYCSFCGKNQEEVKKIIAGNNVFICNECVELAQEI
                     IREELAEEVLTDLADTPKPQELLNILNNYVIGQDRAKRALAVAVYNHYKRINFQDSRD
                     ENDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVENIL
                     LKLLQAADFNIDRAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTVA
                     SVPPQGGRKHPNQEMIHVDTKNILFIVGGAFDGIEEIVKQRLGEKIIGFGHNNRAIDE
                     KESYMQHIIADDIQKFGIIPELIGRLPVFAALDQLTTEDLVRILTEPKNALVKQYQTL
                     LSYDDVELDFDEDALLAIAEKAIERKTGARGLRSIIEETMLDVMFEVPGQDNVKRVRV
                     TKAAVDGKEKPLLETA"
     gene            complement(4727..5239)
                     /locus_tag="ACHU66_001787"
     CDS             complement(4727..5239)
                     /locus_tag="ACHU66_001787"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFR3390053.1"
                     /translation="MTKKIVAIWAQDENGLIGKGDRLPWSLPADLAHFKETTIGHTMV
                     MGRITFDGMGKRALPNRHTIVLTTDKTYQLESERITILHSVEDVLDWYNKQEQTLFVI
                     GGGQIFTAFAPYIETLIVTDIHGQFNGDVYFPKAFPMEKFQLQSANLRPKDEKNPYDF
                     TIKTYERRDG"
     gene            complement(5577..6416)
                     /locus_tag="ACHU66_001788"
     CDS             complement(5577..6416)
                     /locus_tag="ACHU66_001788"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR3390054.1"
                     /translation="MTKADIIFKENIRKIMEEGVFSENARPRYKDGNVANSKYVTGAF
                     AEYDLSKGEFPITTLRPIPIKSAIKEILWIYQDQTNDLSVLQEKYGVQYWNDWEVEGT
                     GTIGERYGAIVKKHDIISKILKQLEENPWNRRNVISLWDYEAFDQSAGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAEELLRRTPSEVEPRLVLNVPDGTNFFDIRAEDFELVDYNPVKPQLKFD
                     LAI"
     gene            complement(6469..6771)
                     /locus_tag="ACHU66_001789"
     CDS             complement(6469..6771)
                     /locus_tag="ACHU66_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390055.1"
                     /translation="MNLTLLKRLNLVLYGIAIFLFVMLFLPIGQWFDIVNVNFKLTFF
                     IIPFFGLASLPTAIYTKNVRQILLSAILVALYFILFSLITALSGLFHLNFYSFFFK"
BASE COUNT         1987 a         1494 c         1319 g         2059 t
ORIGIN      
        1 aaaatatcct tggagaatca taaaaactat ggtacaataa acgaaaaata aaggaggaaa
       61 tatatgccat ttgtacgtat tgatttgttt gaaggacgca cagaagaaca aaagattgcc
      121 ttagcacgtg aagttaccga agttgtatct cgtaatacta atgcgcccaa agaagccatt
      181 catgtcttca tcaatgatat gcctgaaggg acctactacc cacaaggtga aatgaaacga
      241 aaataagaga aaacactctt gcaagaaaat gcaggagtgt ttctttgtat caagtttatt
      301 tcttatcgtc ttcatccatg ctgagaacac tgaggaaggc ttcttgtgga acttcgacag
      361 agccgatggc tttcatccgt tttttaccag ctttttgctt ttctaggagt ttgcgtttac
      421 gggaaacgtc accaccataa cacttggcca aaacgttctt ccgaagagcc ttgatgtcgc
      481 tacgggcaac aattttctga ccgatagctg cttggattgg tacttcaaac tgttgacgag
      541 gaatgatttt tttgagtttg tccacgataa ttttcccacg ttcgtaggca aattccttgt
      601 ggacgataaa gctgagggca tcgaccttat caccattgag gagaatatcc attttcacca
      661 acttggatga acggtattca gaaatctcat agtcgaagct ggcataacca cgagtggacg
      721 acttaagctt gtcaaaaaag tcaaaaacaa tctcagccag tggaatttga taaataacat
      781 tgacacgatt atcatcgatg taatccatag tcacaaagtc accgcgtttg cgttgggcca
      841 attccatgac tgcaccgacg tattcctgtg gtaccatgat ttgtgcctta acgtaaggct
      901 cttcgatagt ggcaatcttg gttgggtctg ggaattcgga tgggttggct acatcaatca
      961 tttcaccgtc cgtcatgttg acatggtaga caaccgacgg tgctgtcatg atgaggtcaa
     1021 tgttaaactc acgctcaatc cgttcttgga taacatccat gtgcaagagg cccaagaatc
     1081 cacaacggaa accaaatccg agtgcctgcg atgtttctgg ctcaaactgt aagctggcat
     1141 cgttgagttg gagtttttcc aaggcttcac ggaggtcatt gtacttgttg gaatcgattg
     1201 gatagatacc tgcaaagacc attggattca tctgcttgta gcccgctagt ggctcgctag
     1261 caggattttt tgccaaagtt accgtatcac cgacacgggt gtcggcaacc gtcttgatag
     1321 aggctgcaat gtaacctaca tcaccagtcg ccaaataatc acgaccgatt gctttgggtg
     1381 tgaagatacc cacttccgtc acatcaaagg ttttgccatt gctcatcatc tgaatagtat
     1441 cgccaggttt taccacaccg ttgacaatac gaacttggag gataacgcct cgatacggat
     1501 cgtaaaccga gtcaaaaatc aaggcttgca atggagcttc gacatcacca gtcggtgctg
     1561 gaaccttctc cacgatctgc tccaagattt cctcgatacc aatacctgcc ttggctgatg
     1621 ttggcactgc ttcggaagca tccaaaccaa tcacatcttc gatttcctga cgaacacgtt
     1681 ctgggtctgc tgctggtagg tcaatcttgt taatgattgg caagatttcc aaatcattgt
     1741 ccaaggctag ataaacgtta gccaaggtct gtgcttcaat cccttgagca gcatccacca
     1801 ctaaaatcgc tccttcacag gcagccaacg aacgcgatac ctcataggta aaatccacgt
     1861 gtcctggtgt gtcaatcaag tggaaaatgt atgtctctcc atctttagct gtatagttga
     1921 gttcgattgc gttgagttta attgtgatac cacgctcgcg ttccaagtcc atactatcta
     1981 gcaactgggc ttgcatttca cggctggaaa ctgtttctgt cttctcaaga atacggtcag
     2041 ccaaggttga tttaccatgg tcgatgtggg caataatgga gaaattacga attttctcct
     2101 gtcgtttttt caaatcttct aaattcatgt ttcttcctct acaaggctat tactcctcat
     2161 tatatcagat ttagtgataa ttttccataa gaaaaacacc tagaaaacta ggtgtctgct
     2221 ctgctatttt cccagccaaa gaccgatctc tcgttcggct gcttctgcac tatctgagcc
     2281 atgaacgaca tttgccacaa ttccctcaac gggcgctgtc gcaaaatctc cacgaatagt
     2341 cccaggtagg gcgtttactg gattggtagc tcccatcata tctcgccaag atttgataac
     2401 ttctggccct tctataatgc cagctatcat cggcccgctc gtcatatatt gtactaattg
     2461 cgggaagaaa ggtttatctg tcaaatgatc ataatgttga ctgattagct cttctgtcgc
     2521 tgtaaccatt tttaaatctc ggatttgaaa tcccctgcgt tcgatacgtg aaagaatttg
     2581 tcctgcaagt ccacgcttaa gagcatctgg tttgatgatg aagaaagtac gttccatgat
     2641 ttttctcttg aactgtttat tagatgttac ttaagatagc atcccatgcc tgatcgtatc
     2701 cgtgacgagt ctcagaagaa aagataacga attgatcatt tttatcaaaa tccaatttct
     2761 ttttaatcat cgattcgtgc ttattccact ttccttgtgg gattttatca gccttcgttg
     2821 ccacaataat aacaggaatt tcataatatt tcaagaaatc atacatttga acatcatctg
     2881 ctgatggctc atgacgcatg tctactaaac tcaccacaac acgtagattt tctcgtgtcg
     2941 tcaaatactc ttcaatcatt ttgccccact tagcgcgctc cgttttggat actttagcat
     3001 agccataacc tggaacatct acaaatcgaa ttttatcatc tatattataa aaattgagtt
     3061 gttgagtctt tcctggtttt cctgacgtac gtgcaagatt ttttcttcct agaagagtat
     3121 tgataaatga ggactttcca acgttagatc gcccggcaag ggcaatttct ggaatgtcat
     3181 cgcccggata ctgggctttt gaaacagcac tgagcaaaat ctcagcatta tgtgtgttga
     3241 tttccatttt atacctttca taaataaggg tagaaaacaa tcagtttcca ccctttattt
     3301 ttttatgcag tttctagtaa aggcttttcc ttaccgtcta ctgctgcttt ggttacacga
     3361 acgcgtttaa cgttatcttg acctggcacc tcaaacatga catccaacat cgtttcttca
     3421 atgatggagc gaagtccacg agcaccagtt ttacgttcaa ttgctttttc tgcaattgct
     3481 aacaaggcgt cctcatcaaa gtcaagctcc acgtcatcat aagacaagag tgtctggtat
     3541 tgttttacaa gagcattctt cggttccgtc aaaatgcgta ccaaatcctc tgttgtcaat
     3601 tggtccaaag ctgcgaatac aggcaagcga ccaatcaact ctggaatgat accgaatttt
     3661 tgaatatcgt ctgcaatgat atgttgcatg tatgattctt tttcatcaat agcacgatta
     3721 ttatgaccaa aaccgatgat tttttcaccg agacgctgct tgacgatttc ttcgatacca
     3781 tcaaatgcac cgccaacgat aaacaggata tttttggtgt ccacgtgaat catttcttgg
     3841 tttggatgtt tgcgtccacc ttgtggtggt acgctggcca cagttccctc gataattttt
     3901 aggagagctt gctggacacc ttcaccagat acatcgcggg taatggacac attttcacct
     3961 tttttggcaa ttttatcaat ttcatccaca tagataattc ctctttctgc acggtcaatg
     4021 ttaaagtctg cagcctgcaa aagtttgagg aggatatttt ctacgtcttc accaacataa
     4081 cccgcctcag taagagcggt cgcatcggca atagcaaacg ggacattcaa acttttagcc
     4141 aaggtctgag ctaggaaggt ttttcctgaa ccagtcgggc caatcataag gatatttgat
     4201 ttttgcaaat caacatcgtt ctcatcacgg ctatcttgga aattgatacg tttgtagtgg
     4261 ttgtaaacgg ctactgctaa agcacgtttt gcacgatcct gtccgatgac gtagttgttc
     4321 agaatattga gtaattcttg tggctttggc gtatcggcca agtctgtcaa aacttcttct
     4381 gccagttcct cacgaatgat ttcctgtgct agttccacgc actcattaca aatgaagaca
     4441 ttgtttcctg cgataatttt tttaacttct tcttgatttt ttccgcaaaa tgagcaatag
     4501 attagctcat gtgtatgctt aacagccatg tgtttcctct ttcatatctc ttatctaaat
     4561 aaaaatccat aaatggcgtg aatactattg accagattgg taaaagcatt cagtaaaaat
     4621 aaaattgcta aaccaaaacg tttcttccga aaggcttgaa tcgcacaaag aacacaaatc
     4681 ccacagagaa atgctgtaaa taaaccgaat aaacttctct ccatgcctaa ccatctcttc
     4741 tttcataggt ttttattgta aaatcatacg ggtttttctc gtcttttgga cgtaaattgg
     4801 cgctttgtag ctgaaatttc tccataggaa atgctttcgg aaaatacaca tcgccattaa
     4861 attgaccgtg aatatctgtc actattaatg tttcaatgta aggtgcaaaa gcagtaaaaa
     4921 tttgtccccc accaatcaca aatagtgtct gctcctgttt gttataccaa tctagaacat
     4981 cttccacact gtgtaaaatg gtaatacgtt cactctctag ttgataggtt ttatccgttg
     5041 tcaaaacgat ggtatgacga ttgggaagag cacgtttacc catgccgtcg aaagttatac
     5101 gtcccatcac catcgtgtga cctattgtcg tttctttgaa atgagctaag tcagctggca
     5161 atgaccaagg aagtctatca ccttttccaa tcaatccatt ttcatcttga gcccaaatgg
     5221 caacaatctt tttagtcatt ttctctcact ttccgtccta tcattctatc aaaaaaacat
     5281 gaaaaatgca cttaccaagt aggccaagcg caaattttta tcatgttgaa actatcacca
     5341 actcctcaga gatagggagg agtccgagat tcgtagaatg tgaacctcgc tggtgctgga
     5401 aagtccccct catcttaaat cgctaagtca aaattaagtt gcggtttaac tggcttcgaa
     5461 agtctgagta tagactgttg aaggttggaa gtgaaacagt tcggggaact gtttcagccc
     5521 gagcctaaaa ataaagaagc gaggtaaaag aacgaagttc ttcactgcta atccagttaa
     5581 attgctaaat cgaattttaa ctgcggttta actggattgt agtccaccaa ctcaaaatct
     5641 tctgcccgaa tgtcaaagaa attggtgccg tcaggaacat ttaagaccaa acgtggctca
     5701 acttcagatg gtgtgcgacg gagtaattct tcagcctgtt caaactgatt atcatagatg
     5761 tgaagattgt tgatgaaata gaaaaacttc ccaaccttcc agccaaaatg tttagcaatc
     5821 atcatctgaa gggctacata ctgcatagcg ttgatatgat gggctaccaa catatcattt
     5881 gaccgttgag ttagagttgc atccaaataa atctccccat ccactcgccg aacgtcaaac
     5941 atggtctgaa aggcacatgg caaaagaccc gcggattggt caaatgcttc ataatcccac
     6001 aaagaaatca cattacggcg gttccatgga ttttcctcca attgcttgag gatttttgaa
     6061 atgatgtcat gctttttaac aattgcacca tagcgttcac cgattgtgcc tgttccttca
     6121 acttcccagt cattccaata ctggacaccg tatttttcct gtaaaaccga caaatcattg
     6181 gtctggtctt ggtaaatcca gagaatttcc ttgatggctg atttaatcgg aataggtcta
     6241 agtgttgtaa taggaaactc ccccttagac aagtcatact cagcaaaggc gcctgtaacg
     6301 tacttggaat tagcgacatt tccatccttg taacgtgggc gagcattttc tgaaaaaacg
     6361 ccctcttcca taatcttacg aatgttttct ttaaaaataa tatctgcttt agtcatggtc
     6421 ttagtatagc aaattctagc aaaaaactcc agttttgtac cggagttctc atttgaaaaa
     6481 gaatgaataa aaattaaggt ggaacaaacc agatagggca gttattaggc tgaacagtat
     6541 aaaatacaag gcaaccaaaa tagcactcaa caatatctga cgaacatttt tagtataaat
     6601 agccgttggt aagctggcta gtccgaaaaa tggaatgatg aaaaatgtta gtttaaaatt
     6661 cacatttaca atatcaaacc attgccctat tggcaaaaat agcatgacga agagaaaaat
     6721 tgcgatacca tacaaaacta gatttaatct ttttagtaaa gtcaggttca taaccgctcc
     6781 ttctaggggc caaatataaa gtagccaata ccgaatatca gaggaaatga gatcattaag
     6841 aagatactaa tcaaaccag
//
LOCUS       DAZQCJ010000099         6852 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000099 DAZQCJ010000000
VERSION     DAZQCJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.100"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(233..760)
                     /locus_tag="ACHU66_001790"
                     /pseudo
     CDS             complement(233..760)
                     /locus_tag="ACHU66_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636114.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(848..1846)
                     /gene="mraY"
                     /locus_tag="ACHU66_001791"
     CDS             complement(848..1846)
                     /gene="mraY"
                     /locus_tag="ACHU66_001791"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR3390056.1"
                     /translation="MQFALMSGLVAFLATVLLIPRFITFYQAKRIEGQQMHEDVKQHQ
                     FKAGTPTMGGTVFLVVAILVSLLFATAFQLLTGGVLAILFILALYGVVGFLDDFLKIF
                     RKINEGLNPKQKLALQILGGIVFYFVHVRGAGGGELNVFGHMVHLGVLYFPFVLFWLV
                     GFSNAVNLTDGIDGLASISVVISLLAYSVIAFNEQKFDILLVCVTMIGGLLGFFVYNR
                     KPAKIFMGDVGSLALGGMLATISIALRQEWTLLLIGLVYVIETSSVMLQVSYFKYTKK
                     RFGEGRRIFRMTPFHHHLELGGLTGKAEKWSEWKVDFFLWSVGLIMSLITLAILYL"
     gene            complement(1848..4097)
                     /gene="pbp2X"
                     /locus_tag="ACHU66_001792"
     CDS             complement(1848..4097)
                     /gene="pbp2X"
                     /locus_tag="ACHU66_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2X"
                     /protein_id="HFR3390057.1"
                     /translation="MPRRNNKLLRYVLKKRYVPSKNRRRVGQSLLLLTVFVFFLFLIN
                     FAYIIGTDSKFGVDLSERADAVHQREVTVQAKRGTIYDRTGIPIAEDSTTYTVYAIID
                     TEYVSELKKVLYVQSSQFSKVAEIFKKHLGMETAYVMEQLNQPELKQVYFGTLGKNIS
                     YSTMSSIQEEMKAAGIEGIAFNASPGRMYPNGNFASIFVGLANPVENKDGSHSLQGVS
                     GLEYYLNDILAGQDGKVIYEKDSQGRVLPGTEKVETETVNGQDVYTTISADLQRSLET
                     NMNTFFDNAKGRYANATLVSAKTGEILATTQRPSYDSDTKEGLGQEGLLERSLLYQEA
                     FEPGSTMKVLTVASAIDNGTFDPNAYYTNNEYVIADAKINDWTLNAGYSAVTLNYAQG
                     FSLSSNVGMTLLQQQMGDEKWLNYLTKFRFGYPTRFGMGDEAPGMVPEDNIVSIAMSS
                     FGQGISANQAQMLRSFTSIANNGIMLEPKFISALYDPNTDTARVSSKEEVGNPVSEQA
                     ADDTLRYMINVGTDPVYGTLYSHDLQGPAIQVDGYDIAVKSGTAEIASEDGQGYIKGA
                     YINSVVAMIPAEDPEFIMYVTIRQPEVLNVLSWRDIVSPTLKEAMLLKDNLNLDSPAP
                     ALARVANETEYQLPDLIGKAPGESAEELRRQLVQPVILGNGAEIKKVSVEKGSKVIAN
                     QQVLLLTNKFEEMPDLYAITKENMDIFAEWTGIEVTYKGTGSKVVDQSVEVGTALNKT
                     KKITITLGD"
     gene            complement(4097..4420)
                     /gene="ftsL"
                     /locus_tag="ACHU66_001793"
     CDS             complement(4097..4420)
                     /gene="ftsL"
                     /locus_tag="ACHU66_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR3390058.1"
                     /translation="MLQEKRREATMRVIGDKIRTFTRVEKAFYSSIVLSGLALAIGII
                     FMQTRLLQLQSGMAKVNQDISQKQIEINDAKQAVNELTRSARLMEIAEKAGLTFNNDN
                     IGVAE"
     gene            complement(4431..5381)
                     /gene="rsmH"
                     /locus_tag="ACHU66_001794"
     CDS             complement(4431..5381)
                     /gene="rsmH"
                     /locus_tag="ACHU66_001794"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR3390059.1"
                     /translation="MTKEFNHTTVLLHETVDMLDIKPNGIYVDATLGGAGHSEYLLSQ
                     LTDGGHLYAFDQDQTAIDHAHIRLASYIEKGQVTFIRDNFRNLKTRLAELGVTEIDGI
                     CYDLGVSSPQLDERERGFSYKQDAPLDMRMNREGHLTAYDVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQARAVKPIETTTELAELIKSAKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAIDLLALDGRISVITFHSLEDRLTKQLFKEASTIDVPKGLPFIPD
                     DLKAPLELVNRKPILPSQEELEANNRAHSAKLRVAKKVHK"
     gene            5652..5840
                     /locus_tag="ACHU66_001795"
     CDS             5652..5840
                     /locus_tag="ACHU66_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR3390060.1"
                     /translation="MNRVKDYRLLLGISQLDLAKAIGVSRQTVNMIENNKYNPSLDLC
                     INLAKALQTDLNSLFWNE"
     gene            5856..6056
                     /locus_tag="ACHU66_001796"
     CDS             5856..6056
                     /locus_tag="ACHU66_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390061.1"
                     /translation="MKKNIRQALISTLIFIPLFVLFQLWGNSGAEILHTISQAKFWLQ
                     LLIAGTIYFLGFVYILPLFDQK"
     gene            6067..>6852
                     /locus_tag="ACHU66_001797"
     CDS             6067..>6852
                     /locus_tag="ACHU66_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HFR3390062.1"
                     /translation="MKKETFQDKLIKRFYGIAGPLDEFRQKEAFRLGNTCFILLFWGT
                     MAITLLALALSKRYPEVVAYGYPTALLLSTLSASMYMTSKMRHSQVDSLDVEELTTKE
                     QKKFKGASIKFALYFTCGMYIWNTGFDAWMEGLNPLDHLFDLRKFLAACLVGVFMGIY
                     IEITLRKRMKKAEKLTVSSAIAKEEPKWIKNMIKRFYGIRGPLDEYRRAEADAIGGQA
                     FIYYFYFLALGNAIAYFLAYRYPLEVAAYYPMIIAFFSIILIGI"
BASE COUNT         1981 a         1600 c         1290 g         1981 t
ORIGIN      
        1 gagttcgtca acaagtcttt atttctagtt gttgagctga aacagtctat ccccagactg
       61 tttcactccc acccccgcac agctcaaact gtctgggaga cagtttgagg ttggagatga
      121 agcgaactct gttcacatca gtcatagagg ccagatttgg agtgtaaaac acgaattgct
      181 gagatgttga cacgaaccct gagaagcgag gttggtcttt ttgcccagcc tacaaagcct
      241 gcatagctac ttttttaccg atattagctc cgactgtctt ggatacttta ttgagaagaa
      301 ccgctgaacc acctgctcca aacatgacac caatgtaggc aataatgcgg atttgaacct
      361 cttctgaaat ttccttccct tcaccaacaa ataattcatc ttcaccaaaa agataagcga
      421 tttgttgcga caaattcaag gcaaaaccaa aatactgagc tatatcagca gtacctgccg
      481 ccaccgctgt aaatggattc cccggtaaac cagctgcgaa agaaatcaaa gatgttttcg
      541 tagtcatatc attaatgatt ttttcagctt ccttacgcaa agcttttgta gtgaagaccg
      601 ctgttggtcc atccgcaata attttatcta gatatggact atctttaaaa cgagtcttca
      661 aaaattcttc ccgattgact ttaaccatag gaagttgact agcagctgtc atggtttgaa
      721 taaccactcc atataactgt tcttgattga attgttccat ttttatcctc caccaattct
      781 ctctatttcc cccccccgac catatttgtc aaggtaaaaa aacagcattt ctgctgtttc
      841 tgacatttta tagataaaga atagctaggg taatcaagct catgatgaga ccgactgacc
      901 agaggaaaaa gtcaactttc cactcgctcc atttttcagc ttttccggtc aaacctccaa
      961 gctccaaatg gtgatgaaaa ggagtcatac ggaagatacg acgaccttct ccaaagcgtt
     1021 ttttggtgta tttaaaatat gaaacctgca acataacaga ggacgtttca atgacataga
     1081 ccaggccgat aaggaggaga gtccattcct gacggagggc aattgaaata gttgccaaca
     1141 taccgccgag agccaagctt cccacatccc ccatgaaaat cttcgctggt ttacggttat
     1201 aaacaaagaa accgagcaag ccaccaatca tggtcacaca aactagaaga atatcaaatt
     1261 tttgctcatt gaaggcgatg acagagtaag ctagaaggct gataacaact gagatagaag
     1321 ccaatccatc aatgccatct gtcaaattaa ctgcatttga aaaaccgacc agccagaaca
     1381 gaacaaatgg gaaatagaga actcccaaat gtaccatgtg accaaataca ttcaattctc
     1441 cgccacctgc tcctcttacg tggacaaagt agaagacaat acctcctaaa atttgtagag
     1501 caagcttttg tttaggatta agaccttcat ttattttacg gaaaattttt aaaaagtcat
     1561 ccaaaaatcc tactacacca taaagggcaa ggatgaagag gatggcaagg acaccacccg
     1621 tcaacagttg aaaggccgtt gcaaacaaca agctgacaag aatcgcaaca acgagaaaaa
     1681 ctgttccccc cattgttggt gtacctgcct taaactgatg ttgtttcaca tcttcgtgca
     1741 tctgttgccc ctcaatgcgt ttggcttgat aaaatgtaat aaatcgtggg attaagagaa
     1801 cggttgctag aaaagctacc aatcccgaca taagtgcgaa ttgcatgtta gtctcctaag
     1861 gttattgtta ttttcttggt tttattcaaa gcggtaccga cctctacact ttggtcgact
     1921 acttttgaac ccgttccttt ataggtcact tcgataccag tccattcagc aaatatatcc
     1981 atattctctt tggtgattgc gtacaagtct ggcatttctt cgaatttatt tgtcaggagt
     2041 agaacctgtt ggttggcgat taccttactt cctttttcga ctgaaacttt tttaatttca
     2101 gcaccgtttc caaggataac tggttgaact aattgacggc gaagttcctc agccgactcg
     2161 cctggtgcct taccaatgag gtctggcaat tgatactctg tctcattagc gacccgtgcc
     2221 aatgctggtg caggtgaatc cagattcaaa ttgtctttca acagcatcgc ttctttcaat
     2281 gtaggactga caatgtcacg ccaagacagg acatttaata cttccggttg gcgaatggtc
     2341 acatacatga taaattctgg gtcctcagca gggatcatgg ctactacaga gttgatatag
     2401 gcgcctttaa tgtatccttg accatcttcg ctggcaattt ccgccgtacc tgatttgact
     2461 gcaatatcat agccatcaac ctgaattgca ggcccctgta agtcatgtga gtagagtgtt
     2521 ccataaacag ggtcagtacc gacattaatc atatagcgaa gtgtatcatc ggcagcttgt
     2581 tcagaaactg gatttcccac ttcttctttc gaagaaactc tagctgtatc tgtattagga
     2641 tcataaagag ctgagataaa tttaggctct agcataatac cattgttcgc aatggatgta
     2701 aaactacgaa gcatctgtgc ttggtttgca gaaatcccct gaccaaacga tgacatggcg
     2761 atagaaacaa tgttatcctc tggtaccatt cctggcgcct cgtcccccat accaaaacgc
     2821 gttgggtagc caaaacggaa cttagtgagg taatttagcc atttttcatc tcccatctgt
     2881 tgctgcaata aggtcatccc aacgttactg gatagggaga agccttgcgc ataattgaga
     2941 gtcacagctg aataacctgc attgagggtc cagtcattga ttttcgcatc tgcaataacg
     3001 tactcattat tggtataata tgcatttggg tcaaaggttc cgttatcgat agctgacgct
     3061 actgttagca ccttcattgt tgaaccaggc tcaaaggcct cttgatataa aagtgaccgc
     3121 tctagcaggc cttcttgtcc cagaccttct ttggtatctg aatcatagct tggcctttgg
     3181 gtcgtcgcca gtatttcacc cgtcttggct gaaaccaaag ttgcattcgc atatcgccct
     3241 ttggcgttat caaagaaggt attcatattg gtttcaagag aacgttgcag atcggctgaa
     3301 attgtcgtat agacatcctg cccgtttact gtctccgttt caaccttttc tgtcccaggt
     3361 aggacacggc cttgactatc tttctcgtag ataacctttc catcttgacc agctagaata
     3421 tcattcagat aatattccaa cccactcaca ccctgtaaac tatgactacc atccttgttt
     3481 tcaaccggat tagctaaccc gacaaaaatt gaagcaaaat ttccattcgg atacatccgt
     3541 cctggactag cattaaaggc aatcccttca atacctgccg ccttcatctc ttcttggatt
     3601 gaggacatcg tgctatagga gatattctta cctaaggtcc caaagtatac ctgcttcagt
     3661 tccggttgat tcagctgctc cataacataa gctgtctcca ttccgagatg ctttttaaag
     3721 atttctgcta ctttgctgaa ctgagaagac tgcacataca aaactttttt cagttctgac
     3781 acatattctg tgtcaatgat ggcatagact gtataggtcg ttgaatcttc tgcaatagga
     3841 attcctgtac ggtcgtatat cgtcccccgt ttagcttgaa cggtaacttc acgctggtgt
     3901 accgcatcag ctctctctga caagtccacc ccaaatttac tatcagtgcc gataatatag
     3961 gcaaagttga ttaaaaagag aaaaaagacg aaaaccgtca aaagaagtag gctctgtcct
     4021 actcttcgac gattttttga cggaacatat cgttttttca agacataccg caagagttta
     4081 ttatttcttc tgggcattat tcggctactc caatattgtc attattaaaa gttaatccag
     4141 ctttttcagc aatttccatc aagcgagctg aacgtgtcaa ttcattgaca gcttgctttg
     4201 catcgttaat ttcaatctgt ttttggctaa tatcttgatt gactttggcc atgccagatt
     4261 gaagttgcaa aagccgcgtt tgcataaaga taattcctat cgcaagggct aatccagata
     4321 atacgatact tgaatagaag gctttttcta cacgtgtgaa ggttctaatt ttatcaccga
     4381 taacccgcat agtggcttct ctacgttttt cttgcaacat agactctctc ctatttatgt
     4441 actttcttag ctacacgcag tttagctgaa tgcgcgcgat tatttgcctc taattcttct
     4501 tgactaggta agataggctt acgattgacc aattcaagtg gcgccttcaa gtcatctgga
     4561 ataaatggca aaccttttgg aacatcaatc gtacttgctt ctttgaagag ttgtttggtc
     4621 aaacgatctt ctaaagaatg gaaagtaatc acagaaatcc gtccatccaa agccaataag
     4681 tcaatggcct gctggataga ttcgtctgct gcccccaact catcgttgac ttcaatccga
     4741 atcgcttgga aaatctgctt ggcaggatgg cccttcttct taagttcctt ggctggcttg
     4801 gcagatttga tcagctctgc caactcagtc gttgtttcaa tcggcttgac cgcacgcgcc
     4861 tgttcaatct tacgagcaat ctgcttggaa aatttatcct caccgtattt aaagaaaatc
     4921 cgtaccaagt cgtgataatc atagttattg accacatcat aagccgtcaa atgtccctca
     4981 cgattcatcc gcatatcaag cggagcatct tgtttgtaag aaaagccacg ctcccgctca
     5041 tccaactgtg gactagatac tcctaaatca taacaaatcc catcaatctc tgttacacct
     5101 agctccgcca agcgagtctt gagattacgg aaattatccc tgataaaggt cacttgtccc
     5161 ttttcaatgt aggatgccaa gcgaatatgg gcatgatcaa tagcagtctg atcctgatca
     5221 aaggcataaa gatgcccacc atccgtcaac tgactgagca ggtactcact atgacccgct
     5281 ccacccagcg ttgcatctac ataaattcca ttgggtttga tgtctaacat atcgactgtc
     5341 tcatgcaaga gaacagttgt gtgattaaat tccttggtca tatcttttct attgtaccac
     5401 aaattaaaat aaaaagttgt tgcaaacttt ttacaaaaaa actgtcaaat atagttgaca
     5461 acttatatga acaggtatat actaacagta aagaaacatg tcacatatag ttgacatcaa
     5521 cagggaggtc tcccacattt ttttacagtt atatgtcaga tataattgac aatattttaa
     5581 aaggatagtc aacctatcct ctcattttta gccaagcatg tcagatataa ttgacataga
     5641 aaggagggca catgaaccgt gtcaaagatt atcgcctact gctaggcatt tcccagctgg
     5701 atctagccaa agccatcggc gtatccaggc agaccgtcaa catgattgaa aacaacaaat
     5761 acaacccctc gctagacctc tgtatcaacc tagccaaagc tttacagact gacctcaaca
     5821 gcctcttttg gaatgaatag aaaggaagaa aaaacatgaa aaagaatata cgtcaagctc
     5881 ttatctctac ccttattttt ataccgctct ttgtcctctt tcagctttgg ggcaatagcg
     5941 gagcagaaat cttgcacacc atcagccaag caaaattttg gctacaattg ctgattgctg
     6001 ggaccatcta cttcttaggc tttgtttaca tcttaccatt gttcgatcaa aaataaggag
     6061 ctgcttatga aaaaagaaac ctttcaagat aaattaatca aacgatttta cggtatcgca
     6121 gggcccttgg atgaatttcg ccaaaaagaa gccttccgac taggcaatac ctgctttatc
     6181 ctgctctttt ggggcaccat ggctataacc ctgctggcac ttgccctatc caaacgctac
     6241 ccagaagtcg ttgcctatgg ctacccaacg gctctactcc tatccactct ttccgccagt
     6301 atgtatatga catccaagat gcgccacagt caagtggaca gtttagatgt agaagaactg
     6361 actaccaagg aacaaaagaa attcaaggga gcaagtatca aattcgccct ctatttcacc
     6421 tgtggtatgt atatctggaa cacgggattt gatgcctgga tggaaggact caatccacta
     6481 gaccatttat ttgacctccg aaaattcctt gcagcctgtc tagtcggtgt tttcatggga
     6541 atttatatcg aaatcactct tcgtaaacgc atgaaaaagg cagaaaaact aactgtcagc
     6601 tctgctattg ccaaagaaga acccaagtgg atcaaaaata tgattaaacg tttttatggt
     6661 atccgcggtc ctttagacga ataccgacgg gccgaggctg atgctatcgg cggacaagcc
     6721 tttatctact acttctactt cctagctctt ggaaatgcca tcgcctactt cctagcctac
     6781 cgctaccctc tggaggtggc tgcctactat ccaatgatca ttgccttctt cagtattatc
     6841 ctaattggta tc
//
LOCUS       DAZQCJ010000100         6777 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000100 DAZQCJ010000000
VERSION     DAZQCJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.101"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            15..1013
                     /locus_tag="ACHU66_001798"
     CDS             15..1013
                     /locus_tag="ACHU66_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR3390063.1"
                     /translation="MVTIKDIAKQAHLSSATVSRVLKGDLTLSVSQETRDRIFNLAQD
                     LGYTKHLKKQAPQQKGTIGIVQWYTESEELADLYYYSIRASIEQTASLLGYQIIRSFN
                     DLTHPLLQEVDGIIAVGKFSSGQIAELTNLSSKLIFVDSDTLTEGFSCITTDFEHSVQ
                     TVIDHFRSQGLTDIGLLVGQEETTDGYQLPTDPRLTAFRDYLDTLGMLQEDYIYQGKF
                     STQSGYELMSQAIEDLGDQLPPAFFMANDTLAVGALRALQERQIAVPGRVQLITFNDT
                     AITRQVYPALSSISVFTEEMGQEAMQLLDRVIASPQPHHPRKIKLGTQLVIRESSY"
     gene            complement(1316..1471)
                     /locus_tag="ACHU66_001799"
     CDS             complement(1316..1471)
                     /locus_tag="ACHU66_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFR3390064.1"
                     /translation="MPMGNSSKSDGKEEHLLTAFEVVQTILGFGSFTIALIGLCYKIF
                     KDNDKKK"
     gene            complement(1789..3228)
                     /gene="gatB"
                     /locus_tag="ACHU66_001800"
     CDS             complement(1789..3228)
                     /gene="gatB"
                     /locus_tag="ACHU66_001800"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFR3390065.1"
                     /translation="MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKDMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIELEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FNYVRKGLQHEVERQAKILRSGGQIQQETRRYDESTGETILMRVKEGSADYRYFPEPD
                     LPLYEIDDSWIDEVRAELPVFPKARRAHYVENLGLTTYDAGQLTSTKALSDFFEAAVA
                     AGGDAKQVSNWLQGEVAQFLNAEGKTIEQIALTPENLVEMIALIADGTISSKIAKKVF
                     VHLAKEGGSAKAYVEKAGLVQISDPAVLIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVAQQLLAQELAKLLD"
     gene            complement(3228..4694)
                     /gene="gatA"
                     /locus_tag="ACHU66_001801"
     CDS             complement(3228..4694)
                     /gene="gatA"
                     /locus_tag="ACHU66_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFR3390066.1"
                     /translation="MTFNNKTIDELHDLLVKKEISALELTKATLEDIKSREGAVDAFL
                     TITEDAALAQAAALDEKGIDADNVMAGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATSVSQAYAKDMIVVGKTNMDEFAMGGSNENSAFKPTKNAWDQTKVPGGSSGGSAAAV
                     AAGQVRLSLGSDTGGSIRQPAAFNGIVGMKPTYGTVSRFGLIAFGSSLDQIGPFSQTV
                     KENAQLLNVISGHDVKDATSTINEIADFTSKIGQDIKGMKIALPKEYMGEGIDPQVKE
                     TILKAAKHLESLGAIIEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGFRAEDA
                     TNLEEIYVKTRSQGFGEEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGLSIPAGFVEGLPVG
                     LQLIGPKYSEETIYQVAAAFEATTDYHKQQPVIFGGAN"
     gene            complement(4694..4996)
                     /gene="gatC"
                     /locus_tag="ACHU66_001802"
     CDS             complement(4694..4996)
                     /gene="gatC"
                     /locus_tag="ACHU66_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFR3390067.1"
                     /translation="MKISEAEVRHVAKLSKLEFSDQETAEFATSLSKIVDMVELLNEV
                     DTTGVAVTTTMADRKNVLRADIAQKGESREELFKNVPETQDNFIKVPAILDGGGDA"
     gene            complement(5214..5888)
                     /locus_tag="ACHU66_001803"
     CDS             complement(5214..5888)
                     /locus_tag="ACHU66_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR3390068.1"
                     /translation="MSEQFIPSVLSDLRQDIVQMPEVIKECSGIRIYGRRIRSVLFTT
                     DVSIIANHNADAILAVYPFTPSPAIIKSIMLVASVPVLAGVGGGLTTGMRSANMSLLS
                     ESEGAYAVVVNGPTDVKTIEAINKVVDIPIIYTVVSEKSDLTSRIKAGVDILNVSCGM
                     ETPRVVKKIREAFPDFPIIATGGPTDDSIRRVIEAGANAVSYTAPSNGELFKGKMEKY
                     RKHAKD"
     gene            complement(6070..6417)
                     /gene="rplS"
                     /locus_tag="ACHU66_001804"
     CDS             complement(6070..6417)
                     /gene="rplS"
                     /locus_tag="ACHU66_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR3390069.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGVGVERTFPIHTPRVDKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
BASE COUNT         1957 a         1674 c         1294 g         1852 t
ORIGIN      
        1 aacaggagaa gaacatggtt acaatcaaag acatagcaaa acaagcccac ctttcctctg
       61 ccaccgtttc ccgcgttctc aagggagacc tgaccctatc cgtcagccaa gaaacccgcg
      121 accgcatttt caacttggca caggacctgg gctacaccaa acacctcaaa aaacaagctc
      181 cccaacaaaa aggcaccatc ggtattgttc agtggtacac cgagagcgaa gagctggctg
      241 acctctacta ctattctatt cgagctagca tcgagcaaac cgccagcctc ctgggctacc
      301 aaatcatccg ctcctttaac gacctgaccc atcccctcct ccaagaggtt gacggcatca
      361 tcgcggttgg taagttttct tctgggcaaa tagcagagct aaccaacttg tcttccaagc
      421 tcatttttgt ggactctgat acactgacgg agggattctc ctgtatcacg accgactttg
      481 aacactctgt acagaccgtt atcgaccact tccgttctca aggtctgaca gacattggcc
      541 tcctagttgg acaggaagaa accacagacg gctaccaact gccaacagat ccccgcctga
      601 ctgcctttcg tgactaccta gacaccttgg gcatgctcca agaagactac atctaccaag
      661 gaaaattttc cacccaatca ggatatgagc tgatgagcca ggctattgag gacctaggcg
      721 accagctacc ccctgccttt ttcatggcaa atgataccct ggcagtcgga gccctgcgtg
      781 ctttacaaga gcgtcaaatc gcagtgcccg gtagagtcca actcatcacc tttaacgaca
      841 cagccatcac ccgccaggtc tatccagccc tgtcttccat cagcgtcttc accgaggaaa
      901 tgggccagga agctatgcaa ctgctggacc gtgtgattgc gagtccgcag ccccaccatc
      961 cccgtaagat taagttgggt acccagctag taatacgcga gagttcttat tgataaggaa
     1021 atcttccatc attccgttac ttatattgaa ttttatacaa gtggtttgat accgagtacg
     1081 gcttttcaat aagtattaaa gatgttaaac ttcttgtaaa acttgtacta aaatttgcat
     1141 agcacatgct catagccaac atgaacagat tatttgcatt ttacaagtaa tctggtataa
     1201 tggatataga aaaaggagct ggacggcaat ccagccccaa atgtagaacc gttaaaaaga
     1261 cggtggctca gtttagatta tttagaaata accgttactg tccaaagttt gacggttatt
     1321 tctttttgtc attatctttg aagattttat agcacaagcc aatcaatgca atagtaaaac
     1381 taccaaagcc tagaatagtt tgcacaactt caaaagctgt caaaagatgc tcctcctttc
     1441 cgtcagattt tgatgaattg cccataggca tcacctcact ttcagaaaat caagagccac
     1501 cgtcttcact tttctaccca tctagtataa catactcatt cctaaaaatt attggaatag
     1561 tgcagtattc ccgaaatctt tgagaacccc caaaaaatta caactcttct atagtgatga
     1621 tattttttca ttttagctaa ctatccttta acagtcaata cccgcgctac cttctaatac
     1681 tttataccgt ttttaaaata tagcataaaa ataagataga agcacctagt ttacaagtaa
     1741 cagtaaaaac cgctaagatt caatcctagc ggttttcttc tattcaattt aatccaacaa
     1801 cttagccaat tcctgagcca agagttgttg tgcaacttgc gggttagctt gtcccttagt
     1861 cgctttcatc aagaaaccag taaaggcctt gtctgcattg cgtttacctg acttgaagtc
     1921 agctacagcc gcttcgttat ccgcaaagac ttggtggata atcggaatca agaccgcagg
     1981 gtctgaaatc tgtaccaaac cagccttctc aacataagcc ttagcagagc cgccttcctt
     2041 agccaagtga acaaagactt tcttagcaat tttggatgaa atagtgccat ccgcaatcaa
     2101 ggcaatcatc tcaaccaagt tttctggtgt caaagcgatt tgctcaatag tcttaccttc
     2161 agcattgagg aactgagcca cctcaccctg caaccagttg gatacctgct tagcatcgcc
     2221 acctgctgcc actgctgctt caaagaagtc agacagagcc ttggttgatg tcaactgacc
     2281 agcatcgtag gtagtcaagc ccaagttttc cacatagtga gcacggcgag cctttggaaa
     2341 gactggcaat tctgcacgca cttcatcaat ccagctatca tcaatctcgt agagcggtag
     2401 gtctggctct gggaagtaac ggtagtccgc tgaaccttct ttgacacgca tgagaatggt
     2461 ttcaccagta gattcatcgt aacgacgcgt ttcctgctgg atttgaccac ctgaacgcaa
     2521 gattttggcc tgacgttcta cttcgtgttg caagcccttg cgaacatagt tgaaggagtt
     2581 gaggtttttc aactcagtct tggtaccaaa tttttcttga ccatagggac gaagagagat
     2641 gttggcatcc acgcgcatag aaccctcttc catcttcacg tctgaaatac cagtgtattg
     2701 gataatttcc ttgagagcag tcaagtaggc ataggcctcc tcaggcgaac gcatatcggc
     2761 ttctgataca atctcaatca gtggcactcc ttgacggttg aggtccacat aagaataacc
     2821 gtctgtcccg tgggtattct taccagcatc ctcttccaag tgggcacgct cgatacggat
     2881 tttcttggtc gaaccatctt ctaactcaat ctcaatccag ccattgtagc caatcggctc
     2941 gtcaaactgg gaaatctgat aggctttagg gttatcagga tagaaatagt tcttacggtc
     3001 aaagtgcatg tccttatgaa tgtccatgtt caaagccaag gcagccttga ttcctgcgtc
     3061 cacaacgccc ttattaagaa caggaaggac acctgggaaa gaccagtcaa tcacgttggt
     3121 attggcattt ggatcctcac caaaatgagc agatgaaggt gagaaaattt tcgagttggt
     3181 attcaactcc acatggactt ctagaccaat aatcgtttca aagttcatta gttagcacct
     3241 ccaaaaatca ctggttgttg cttgtggtag tctgttgtcg cttcaaaggc agcagccact
     3301 tggtaaatgg tctcttctga atattttgga ccaatcaact gcaaaccgac tggcaagcct
     3361 tcaacaaaac cagcaggaat agaaagacca ggaagacctg ctaagtttac aggaattgtt
     3421 aacaagtccg ccaagtacat agccacaggg tcatggttga gcgtgtccaa accaaaggca
     3481 accgtcggag ctgttggccc caaaatcaag tcatagtctg caaagacttt ttcaaaatcc
     3541 tggataatca aggtccgtac ctgaccagcc ttcttgaagt aggcatcgta gtaaccagat
     3601 gacaagctaa atgtacctaa cataatacga cgtttgactt cctcaccaaa accttggcta
     3661 cgggttttca catagatttc ttccaagttg gtcgcatctt ctgcacggaa accataacgg
     3721 ataccgtcaa aacgttgcaa gttagaagac gcctctgatg aagcaatgat gtagtaaaca
     3781 gcaaccccat acttagaatg tggcaggctg acttcttcaa tgattgctcc caagctttcc
     3841 aagtgcttag ccgccttgag aatagtttcc ttcacctgcg gatcaatccc ttctcccatg
     3901 tattcttttg gcagagcaat cttcataccc ttgatgtcct gaccaatctt gctagtaaag
     3961 tctgcaattt cattgatggt tgatgttgca tccttgacat catgaccaga gatgacattg
     4021 agcaactggg cattttcctt aaccgtctgt gagaatggac cgatctggtc aagagatgaa
     4081 ccaaaggcaa ttaaaccaaa acgtgacacc gttccatagg tcggtttcat accgacgata
     4141 ccattaaagg ctgctggttg acggatagag ccacctgtgt cagaaccgag tgataaacgg
     4201 acttgaccag ccgcaactgc agcagcggag ccacctgaag aaccaccagg aacttttgtc
     4261 tggtcccaag catttttggt cggtttgaag gctgagttct cattagaacc acccatagca
     4321 aactcatcca tgttggtctt accaacaaca atcatatcct tggcataggc ttgggaaacc
     4381 gatgtcgcat cgaaaatcgg ctcgtagtta tagagcattt ttgaagctgc cgtggtcaaa
     4441 atccccttag tagagatatt gtccttgact gccaaaggaa tccctgccat aacattgtcc
     4501 gcatcaatcc ctttttcatc aagggcagca gcctgcgcca aggccgcatc ttctgtgata
     4561 gtcaagaaag catccactgc tccctcacgg ctcttaatgt cttccaaagt tgccttggtc
     4621 aactcaagag ccgaaatctc ctttttgaca aggaggtcat gcaattcatc aatggtttta
     4681 ttgttaaagg tcattaggca tctcctcccc cgtctagaat agctggtacc ttgataaagt
     4741 tatcttgggt ctcaggcaca tttttaaaga gctcctcacg gctctcacct ttttgggcaa
     4801 tatctgctcg caagacattc ttacggtcag ccatggtcgt tgtcaccgca acacctgtcg
     4861 tatccacttc attgagcaac tcaaccatat cgacaatctt gctcaaactt gtcgcaaatt
     4921 ccgctgtttc ttgatccgaa aattccagct tagacagctt ggcaacgtga cggacttcag
     4981 cttcagaaat cttcattctt actttcctcc taatggaaat ctatcttctc attataccat
     5041 atttccagcc ccagacaaag gtagaaatga cctaatatgt taccaatttc ctcctaaaaa
     5101 tccattcttt aaattgcgaa gtattcctaa tctcacaccg taaaacgata aaaaacagca
     5161 agtttcatct agcaataaaa cttactgttt gcttagccta tcttttttat gagctaatcc
     5221 tttgcatgtt tgcggtattt ttccattttc cctttaaaaa gctccccatt acttggtgct
     5281 gtataggaga cagcattggc tccagcttcg ataacccgac gaatagagtc atcagtcgga
     5341 ccaccagtcg caataattgg aaagtcaggg aaagcttctc gaatcttctt cacaaccctt
     5401 ggcgtttcca taccgcaaga aacatttaaa atatctactc cagctttaat ccgtgaggtt
     5461 aaatcagact tttcagaaac aacggtataa atgataggaa tatctactac cttattgatt
     5521 gcctcaattg tttttacatc cgttggtcca tttaccacaa cagcgtatgc tccctccgac
     5581 tcagacaata gactcatatt agctgaacgc atacctgtcg tcaagcctcc accaacacca
     5641 gccaagactg gaactgacgc aaccaacata atacttttga taatagctgg actgggcgta
     5701 aatggataaa cagccaaaat agcatctgca ttgtgattgg caataataga cacatccgtt
     5761 gtaaataaga ctgaacgaat gcgacgacca taaatacgaa ttccactaca ttccttgata
     5821 acttctggca tctgaacaat atcctgccgt aaatctgata aaacagatgg aataaactgt
     5881 tcgctcatcc gcaacctcct ctatactagc tacaataata tatagtataa catattattt
     5941 catggaagat acacaaaaac agtccatttt accactgctt tcacaattaa atactttttc
     6001 aacagattaa aaaaaaagac agctttcgct gcctcccatt ctccgaccaa aatagtctgt
     6061 agaactatct taacgacgga tttctttgat acgtgctgct ttaccttgca atgcacgtag
     6121 gtagtacaat ttagcacgac gtactttacc gtaacgtact acttcaatct tatcaacacg
     6181 tggagtgtgg attgggaatg tacgttcaac acctacacca ccagagattt tacgtacagt
     6241 gtacatttct gagatgcctt gacctttacg agagataaca acaccctcga aaatctggat
     6301 acgttcgcgg ttaccttcga caaccttagc gtgaacacgc acagtgtcac caggacggaa
     6361 tgaagggatg tcagtacgaa gttgaccttc tgtcaaactt tggatcaatg gattcatttt
     6421 attctcctat ctttgtcaat cataggtgct tttaaaccca gcggataaac tgtatttttg
     6481 tgcgtccatt acacacagac tatattatac accaagtaat cacctatgta aagcattttg
     6541 ataaatttat catggtcaat cacaccgctt cttttacaag ttaagtcaac aaggtctgag
     6601 tttgattttc gaagagtgtt aataatacag attcctattg gagaaaaatg aaaagaggct
     6661 gagcaaaaag accaacctcg cttctcaggg ttcgtgtcaa catctcagcg cagtggttga
     6721 ttggcagatt tgttcgtgtt ttacactcca aatctgacct aatcaactgt gcgaggg
//
LOCUS       DAZQCJ010000101         6754 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000101 DAZQCJ010000000
VERSION     DAZQCJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.102"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            117..917
                     /locus_tag="ACHU66_001805"
     CDS             117..917
                     /locus_tag="ACHU66_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFR3390070.1"
                     /translation="MKKILALAATVLAGLTLAACSSTSSQSALDKIKEKGTLVVATSP
                     DYAPFEFQALVDGKNEVVGADIMLAQKIADELGVKLEVSAMSFDNVLSSVQNGKADIA
                     IAGLSYSEERAKVFDFSESYYQISDVLLIKKDSANSLTSIDAMSGKTLAVQKGSTQES
                     YAQENISQANLISLTLMGEAVNELKSGKVDAILMDSPVAAGYVSQNSDLAVASVEFPT
                     IDENSKVIALPKGSTELKTAIDKVIAEVKASDEFDTFLEKAATYASVE"
     gene            complement(1045..2883)
                     /gene="glgB"
                     /locus_tag="ACHU66_001806"
     CDS             complement(1045..2883)
                     /gene="glgB"
                     /locus_tag="ACHU66_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HFR3390071.1"
                     /translation="MTEFTDLDLYYMNAGEHTTLYEKMGAHIIKERNKIIGTQFRVYA
                     PNAREVFLVGNFNEWQRSHSMEREIDGVFQLYVDGLKSLEDYKYLIITHDGREIYKAD
                     PYAFFSQVRPDTASTTYNSRYKFKDDLWMYNRVNYDFKEQPVSIYEVHLGSWKQKFAN
                     KNEGGAPVEAFNSYKEITPLLIEHIKENNYTHIELLPITEHPLDASWGYQVTGYYSPT
                     SRYGKPDELKYLVDQCHQAGIGVILDWVPLHFCKDAHGLYQFDGSWLYEYSYEQDREN
                     HQWGTANFDLGKGLTRSFLLSNLKYWLEYFHFDGIRVDALSYLLYWRGETDEDKINHA
                     AIDFIKRVNAMVHTDYKGVLMIAEDSSSFPKVTHSLEDGGIGFDMKWDLGWMNDTLKF
                     VERPAIYRKYHSNEITFGMYYNQNEQFLLPLSHDEVVHGKHSIIEKMNGDYEDKFHLA
                     RVYYSFYFAHPGKKLLFMGNEWGHIREWHEYTEMDWGLLQFPIHHSFYQMMKTLSTFY
                     KEHDAFWKYDYQAYEKGFNWVKIDEEASLYAFSRTSDVQEILTIHNFNDQELFDVHLD
                     LPADSEYKLVFSSNAIPMDTFSLYPDENGARITVPRLTSFYLERVK"
     gene            complement(2956..4401)
                     /gene="glgA"
                     /locus_tag="ACHU66_001807"
     CDS             complement(2956..4401)
                     /gene="glgA"
                     /locus_tag="ACHU66_001807"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR3390072.1"
                     /translation="MTKKSVLFVASEGLPFIKTGGLADVIGSLPKELVKQGLDVRVVL
                     PLYKKIAVNNHSDFDYVSSFDVRAGDIQSMANVYSQVVDGVTFYFIEHRDFFERDELY
                     GYDDDAMRFGYFQHATCRLLEALHFFPDVMHTHDWHTAALPFLCRTFYSYREEFRNIK
                     HVFTIHNLAFQGIFHKQALWSALGMDYSYYLDGIARFHDECISFMKLGILYADKVTTV
                     SETYAREILTEEFGENMQHVLELRKYDLVGIVNGIDYDSWDSQTDQYLVRNYSLETIE
                     SKKANKLALQAQFALPQDENVLLIGIVSRLTWQKGFYLLTEVLGHLLQAHVQFVILGN
                     GEEDIENAFNHFKHAYPDKFAFYRGYNEPLAHQIYAASDLFLMPSMFEPCGISQLISM
                     HYGTLPLVRETGGLVDTVAPYNMSTKEGTGFSFGGRDAYNMRQVYDLALQTYYDRPDD
                     WYAMVEQAMKRDFSWTASAEKYIWLYREISG"
     gene            complement(4410..5525)
                     /gene="glgD"
                     /locus_tag="ACHU66_001808"
     CDS             complement(4410..5525)
                     /gene="glgD"
                     /locus_tag="ACHU66_001808"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HFR3390073.1"
                     /translation="MLRNTLGIVNIEGNNVHFGDVMKHRGVQAFGFLGRYRLIDFALS
                     NMSNSGITEFQVYMPAEMRSTIQHVGTGKHYNINSKRGTLRLLNSATDPNSVYRHDIN
                     AFTENIHYIENSTKDYVLVAPSYFIYSQDFSKVMDDHIATDADITVLYKNVSDAKENF
                     IGCQTLKFGEDHRVVAFEENHGKYKNRPVSLEAYIMKRTTFIDLIKRANKVSSLYWLK
                     DILRDTVDQYKIMGYAHRDYVACINTTASYFNTQIELIDQDTRKLLFKHDWPIHTQTS
                     DSSPCLYGPLAEVKRSLISNGANINGQVENSVIDRDVIIEEGVVIKNSIILNGVTIKS
                     GANIENAIIDKATKIIHPIDIKGSETSPSYLKPHQII"
     gene            complement(5538..6665)
                     /locus_tag="ACHU66_001809"
     CDS             complement(5538..6665)
                     /locus_tag="ACHU66_001809"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR3390074.1"
                     /translation="MAQNKMLAMILAGGRGTRLEGLTKKVAKPAVAFGGKYRIIDFPL
                     SNCANSGIDIVGVLTQYEPVLLNSYVAQSQRWGLDVQGSGVFVLPPSEKIEGFGLYKG
                     TADAITQNIDFIDLHDPEYVLILSGDHIYKMNYDKLLDTHIQKKADATIAVIEVPIKE
                     ASRFGIMNTDEDYRIEEFEEKPENPKSNLASMGIYIFTWKTLKKYLQEDDKLDTSSHD
                     FGHDIIPKYLEDGRTLIAHPFRGYWKDVGTVNSLWESNMDLIDHAGDLDLSDRSWRIY
                     SEDKGSPAQVIGATATVKSAYIDKGAIIDGTVEHSVISTDVQVNQNAVVKNSVILPGA
                     IIGEGVELDYVIVAENIKIADGVKLTGDIDHILLIDKNVTK"
BASE COUNT         2015 a         1294 c         1387 g         2058 t
ORIGIN      
        1 tgaattaaaa tgagaataca ttctgaaaaa ctgcaagaaa atacaatgaa aatcaataag
       61 cttgtgtttt ttagaataag tattttatta aaataaagag aaggtattta ggaaatatga
      121 aaaaaatctt ggcattagca gcaacagttt tagctggact gacattggca gcttgttctt
      181 cgacgtcatc acaatctgca ttagataaaa ttaaagaaaa aggaacatta gtagtggcaa
      241 ctagtccaga ctatgctcca ttcgagtttc aggcactggt tgatggaaag aatgaagttg
      301 ttggtgcaga tattatgttg gctcaaaaaa tagccgacga acttggggtc aagcttgaag
      361 tttcagcaat gagttttgac aatgtattgt ctagcgttca aaatggcaaa gctgatattg
      421 caattgctgg tctatcttat tcggaagagc gtgcaaaagt ttttgatttc tctgaaagtt
      481 actatcaaat ttctgatgta ttacttatca aaaaagatag cgccaattca ctcacttcaa
      541 ttgatgctat gagcgggaaa actctcgcgg tacaaaaggg ttcaacacaa gaatcctatg
      601 cacaggaaaa cattagtcaa gctaacctaa tttctttaac cttgatgggt gaagctgtaa
      661 atgagctcaa atcaggaaaa gttgatgcta ttttaatgga ttctccagtt gctgctggtt
      721 atgtttcaca gaattcagat ttagccgttg catccgttga atttccaacg attgatgaga
      781 attcgaaagt cattgctttg ccaaaaggta gtacagaact aaaaactgcg atagacaagg
      841 ttatcgcaga agtaaaagca agtgatgagt ttgatacttt ccttgagaaa gctgcaacat
      901 atgcaagtgt tgaataagag aaatgaacaa tcatcggaaa cgatggttgt ttttgcatgc
      961 aaaaaagaat ggagtaagtg agccacaccc caaaagttag acagaaaatt tgggtcggtt
     1021 caatactcca ttttttatag ataattattt cacacgctct agataaaaac tggtcaaacg
     1081 tggtacagta attctggctc cattttcatc gggatagagg ctaaatgtgt ccattggaat
     1141 agcattagat gagaagacaa gtttatattc gctgtcagct ggcaaatcca agtgtacatc
     1201 aaatagttct tgatcattaa agttatgaat agtcaatatt tcttgcacat cactagtgcg
     1261 agaaaaagca tacaggcttg cttcttcatc tatttttacc caattaaatc ctttttcata
     1321 ggcttggtag tcatatttcc agaaagcatc atgttcttta tagaaagtag atagtgtttt
     1381 catcatttga tagaaagaat ggtggattgg aaattgtagc agtccccagt ccatttctgt
     1441 atattcgtgc cattcacgga tgtgacccca ttcatttccc atgaagagta atttctttcc
     1501 aggatgggca aagtaaaagc tgtagtagac tcgagccaga tggaacttat cttcgtagtc
     1561 gccattcatc ttctcgataa tggagtgctt cccatgaaca acttcgtcgt gcgaaagtgg
     1621 tagtaagaat tgctcgtttt ggttatagta cataccaaaa gtgatttcat ttgagtgata
     1681 ttttcgataa atagcagggc gttccacgaa ttttagtgta tcattcatcc agcccagatc
     1741 ccatttcata tcaaatccaa taccgccatc ttctagagaa tgtgtgactt tagggaagct
     1801 tgaagagtct tcagcaatca tcaggactcc cttatagtcg gtatggacca tggcattgac
     1861 acgtttgata aagtcaattg cggcatgatt tatcttatcc tcatctgttt caccacgcca
     1921 atagaggaga taagatagtg catcaacacg gatgccatca aaatggaaat attccaacca
     1981 gtattttaaa ttagaaagaa ggaaagaacg agtcagtcct tttccaaggt caaaattagc
     2041 agtcccccac tggtggtttt cacggtcttg ttcgtaggag tattcgtata gccaactacc
     2101 atcaaactgg tagagcccat gagcatcttt acagaaatga agtggtaccc agtcaagaat
     2161 cactccgatt cctgcttggt gacattggtc aactaaatac ttaagttcat ctggcttgcc
     2221 atagcgacta gttggactgt agtagcctgt tacctgatag ccccaagacg catcgagagg
     2281 atgctcggta attggcaata actcaatatg tgtgtagtta ttttccttga tatgttcgat
     2341 gagaagtgga gtaatttcct tataactatt gaaagcttct acaggagctc ctccttcatt
     2401 tttattagca aatttttgct tccacgaacc taagtgcacc tcgtagatgg atactggctg
     2461 ttctttaaaa tcatagttaa cacgattata catccagagg tcatctttga atttataacg
     2521 cgaattgtag gttgtagagg cagtgtctgg acgaacttgg ctgaaaaatg cataagggtc
     2581 agccttatat atttcacgcc catcgtgtgt gataatcagg tatttataat cttcaaggga
     2641 ttttaatccg tctacataaa gttgaaatac tccatcaatt tcacgctcca tagagtgact
     2701 gcgttgccac tcgttaaagt tgccaactaa gaatacttct ctggcatttg gggcatagac
     2761 acgaaattgg gttccgataa ttttgtttcg ttcttttata atatgagcac ccattttttc
     2821 gtaaagagtg gtatgctcac cagcattcat ataatataaa tctaggtcag taaattctgt
     2881 catagcaatc tccattgccg aaaaggagtg gactagggtc ccactccgat tcagtatatt
     2941 ttttcatctt atttcctaac cactaatttc acggtagagc cagatgtatt tctctgccga
     3001 agctgtccaa ctaaaatcac gcttcatagc ttgttcaacc atggcatacc agtcatcggg
     3061 acggtcatag taggtttgaa gggctaaatc atagacttgt cgcatattgt aagcgtcacg
     3121 ccctccaaag ctaaaaccag ttccttcctt ggtagacata ttgtatggtg ctacagtatc
     3181 aaccaagcca cctgtttcac gcactagagg gagtgttccg tagtgcattg aaatcaactg
     3241 gctaatgccg catggttcaa acatagaagg catgaggaag agatcgcttg ctgcataaat
     3301 ttggtgggca agtggttcat tgtaaccacg gtagaatgca aatttatcag gataagcgtg
     3361 tttaaagtga ttaaaggcat tttcaatgtc ttcctcacca ttgcctaaga taacaaattg
     3421 gacatgggct tggagaagat ggccaagtac ctcggtcaag aggtagaacc ctttttgcca
     3481 tgtcaaacga gagacgatac ctatcaatag gacattctca tcttgtggaa gggcaaattg
     3541 tgcttgcagg gccagtttat tagccttttt actttcaatc gtttcaagac tataatttct
     3601 cacaaggtac tggtctgttt gactatccca actgtcatag tcgataccat tgacaatacc
     3661 aaccaagtca tatttgcgca actctagtac atgttgcatg ttttcgccga attcttccgt
     3721 taggatttcg cgagcataag tttcagaaac tgtagtaacc ttgtcagcgt ataggatacc
     3781 aagcttcatg aagctgatac attcatcatg aaaacgtgca ataccatcca aatagtagct
     3841 atagtccatg ccaagcgctg accaaagtgc ttgtttgtgg aaaatacctt gaaaagccaa
     3901 gttatgaata gtgaatacat gcttgatatt gcggaattct tcgcggtagc tatagaaagt
     3961 acggcaaagg aatggaaggg cagcagtgtg ccagtcatgt gtgtgcatga catccgggaa
     4021 gaaatgaagt gcttctaaca gacgacatgt agcatgttgg aaatagccaa aacgcatggc
     4081 atcatcgtca tatccatata gttcatcgcg ttcaaagaaa tcgcggtgct caataaaata
     4141 gaatgttact ccatcaacaa cctgactgta aacatttgcc atagattgga tgtcgccagc
     4201 ccgcacatca aaacttgata cgtaatcgaa atcagagtga ttgtttactg caattttttt
     4261 gtagagtggt agaacgactc ggacatctaa accctgtttt accaattctt ttggtaaaga
     4321 gccaataaca tcagccaatc cgccagtctt gatgaatgga agtccctcgg atgctacaaa
     4381 gagaacagat ttttttgtca tgttagctcc taaatgattt ggtgtggttt caagtatgaa
     4441 ggcgaagttt cgcttccctt gatgtcaatt gggtgaataa ttttagttgc tttatcgata
     4501 attgcatttt caatattcgc cccactcttg attgtcacgc cattgagaat gattgagttt
     4561 ttgataacaa caccttcttc aatgataaca tcgcggtcaa tgactgaatt ttcgacttga
     4621 ccattgatat tcgctccatt tgaaattagg ctacgtttga cctctgccaa tggaccatac
     4681 aaacatggtg agctatcact tgtttgggta tggattggcc aatcatgttt gaagaggagt
     4741 ttgcgggtat cttggtcaat caattcaatt tgagtattga agtaggaagc ggtcgtattg
     4801 atgcatgcga cataatcacg atgtgcatag cccataatct tgtactggtc aactgtatca
     4861 cgtaaaatat cttttaacca gtagagagac gacactttgt tagcacgttt aatcaagtcg
     4921 ataaatgtag tacgtttcat aatgtaggct tcaagggaaa cgggacggtt tttatattta
     4981 ccgtggtttt cttcaaaagc tacgacacga tggtcttcac caaatttgag agtttggcag
     5041 ccgatgaaat tttctttagc atctgaaacg tttttataaa gaacggtaat atcagcatcc
     5101 gttgcgatat ggtcatccat gacttttgaa aaatcctggc tatagatgaa gtaagatgga
     5161 gcaactagca cataatcttt tgtagaattt tcgatgtagt ggatgttttc tgtgaaggca
     5221 ttaatatcgt gacgatagac actatttggg tctgttgcgc tattgagcaa gcgaagggta
     5281 ccgcgtttgc tgttgatatt atagtgtttc cctgtaccaa cgtgctgaat tgttgaacgc
     5341 atttcggcag gcatgtatac ttggaattct gtaattccag agtttgacat attggataga
     5401 gcaaagtcaa ttaggcgata acgacctaag aaaccgaaag cttgtacgcc acggtgcttc
     5461 atcacgtcac caaaatgtac gttgtttcct tcaatattta caattccgag agtatttctt
     5521 agcatagcag tcctttctta cttggtcaca ttcttgtcaa tcaagaggat atggtcgatg
     5581 tctcctgtga gtttaactcc gtcggcaatt ttaatatttt cagcgacgat gacgtagtca
     5641 agttcaacac cttcgccgat aatagcacca ggtaggatga cagaattttt tactacggca
     5701 ttttggttga cttgcacatc tgttgaaata acagaatgtt ctacagtacc atcgatgatt
     5761 gcccctttat caatataggc agatttaacc gttgcagttg caccgatcac ctgagcaggt
     5821 gagcccttgt cttctgaata gattctccat gagcggtcag aaaggtccaa atctccagcg
     5881 tgatcaatca agtccatatt ggattcccag aggctgttaa cggtacctac gtctttccaa
     5941 taaccacgga atggatgggc aatgagagtg cggccatctt ctaagtattt cggaatgatg
     6001 tcgtgcccaa aatcgtgaga tgaagtgtcc aatttgtcat cttcttgaag gtactttttc
     6061 aaggtcttcc aagtgaagat atagataccc attgatgcta aattgctctt aggattttct
     6121 ggtttttctt caaattcttc aatacggtaa tcttcgtccg tattcatgat accaaaacgt
     6181 gaagcttctt tgatcggaac ttcgataacc gcaatcgtgg catctgcttt tttctgaata
     6241 tgtgtatcaa ggagtttgtc gtagttcatc ttatagatgt ggtcaccaga caaaatcaat
     6301 acgtactctg gatcgtgaag atcaataaaa tcaatgttct gtgtaatagc gtcggctgta
     6361 cctttgtaaa gtccgaaacc ttcgattttt tcactcggtg gtaatacaaa gacgccagat
     6421 ccttgtacat caagccccca acgttgagac tgtgcaacgt atgagttcaa gagaacaggc
     6481 tcatactgag tcaaaactcc gacaatatca atacctgagt tggcacagtt actgagagga
     6541 aagtcaataa tgcggtactt tccgccaaat gcgaccgctg gtttggcgac tttttttgtc
     6601 aacccttcta gacgtgttcc acgtccacca gcaagaatca tagctaacat tttattttga
     6661 gccatctaac cactccttaa tatatatgat acatgtatta tagcatagag tataaaaaaa
     6721 tgaaagcgtt tccttaaaga aaagtgaatt attt
//
LOCUS       DAZQCJ010000102         6543 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000102 DAZQCJ010000000
VERSION     DAZQCJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.103"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            21..164
                     /locus_tag="ACHU66_001810"
     CDS             21..164
                     /locus_tag="ACHU66_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390075.1"
                     /translation="MKRFELRENLVWQRATAGEKEKLLDTGLADKAGYIRLVRELGRK
                     YVA"
     gene            175..303
                     /locus_tag="ACHU66_001811"
     CDS             175..303
                     /locus_tag="ACHU66_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390076.1"
                     /translation="MDGTYPWFDYDRDYLQPEEPRQVHDPDEWVFRGGQWIYVGDA"
     gene            303..482
                     /locus_tag="ACHU66_001812"
     CDS             303..482
                     /locus_tag="ACHU66_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390077.1"
                     /translation="MTEELLDTIRRLRCDYFHLGRELGKIINEQQDLILALKRENERL
                     KREKWNLKQTKRRKK"
     gene            479..1249
                     /gene="bet"
                     /locus_tag="ACHU66_001813"
     CDS             479..1249
                     /gene="bet"
                     /locus_tag="ACHU66_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFR3390078.1"
                     /translation="MSNNQLSTQQAKRDIAIDTSVWTFQDVKRYFDPQNLLTEKQVGQ
                     ALSLIKGRNLNPLANEVYIVAYKKKTGGTEFSLIVSKEAFLKRAAQNPNYEGFEAGVV
                     TVDTDGVMHERKGALMLPGDTLVGGWARVYRKNFKVPVEIFVSREEYDKKQSTWNAMP
                     ATMIRKTALVNALREAFPEDLGNMYTEDDGGETFDRIKQAEPVESREDVMARKMAQIE
                     QMKQEQAQRQIDTSYPTDDVIDPDDEPAQGELLEDLEY"
     gene            1259..2287
                     /locus_tag="ACHU66_001814"
     CDS             1259..2287
                     /locus_tag="ACHU66_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HFR3390079.1"
                     /translation="MQELQAKVTQAQVEIIDREKFEQGIADVVAKYENYTVTAATIKD
                     DKQVLADLRKLKKQISDERIKIKRELSQSADEFDKYIKGTSEPMDKVIDKIATDVKAF
                     EEHQKAVRLDTVKSYLANKAAEYMLDPRLFDEKALEYIKASDFMADGVTLKKVTMKSL
                     EDMVTFEYQKQQEHEKEKAAISGQCAEYGMTDQPYIRMLQSMSLADVMQQIMSDYLFE
                     QEKQKLRQAEAEREQLSAEQQAKQQEQVQKSPETPKIDPETGEILGGGQLYHPDQKTL
                     RGAENEPKKYTQKMTLEVYFANTAEKDLFKTGLSELGFEHKQNYQVSGYQRIQPVTQE
                     QLNELCGW"
     gene            2290..2559
                     /locus_tag="ACHU66_001815"
     CDS             2290..2559
                     /locus_tag="ACHU66_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390080.1"
                     /translation="MDKQVKQVIDELEPFNHGITIAIHQNKNECIATFRMPRQFDTKK
                     IKFTGWNEDVRNRTSCHSENDLLEAYVYKVWNVSNDWICIEVLPF"
     gene            2569..2895
                     /locus_tag="ACHU66_001816"
     CDS             2569..2895
                     /locus_tag="ACHU66_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390081.1"
                     /translation="MEIRTVSDSVTIYSDGKRLQVIHDLGDEFVLDFGYRTENVVNID
                     ELSPGIVGSIVPVFKVSGFCSKGGEGMHSLRWAILQFQIFENYIKDNQADLLDWYKNP
                     GGEDGN"
     gene            2885..3064
                     /locus_tag="ACHU66_001817"
     CDS             2885..3064
                     /locus_tag="ACHU66_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390082.1"
                     /translation="MATNEIEISNEQALMGTQLQIGKQVMMALLELHSDSKKGGIILP
                     IKLNDIDFNVKIERD"
     gene            3167..3628
                     /gene="ssb"
                     /locus_tag="ACHU66_001818"
     CDS             3167..3628
                     /gene="ssb"
                     /locus_tag="ACHU66_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019778239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR3390083.1"
                     /translation="MINNVVLVGRMTKDAELRYTPSNVAVATFTLAVNRNRKNENGER
                     EADFINCVIWRQAAENLANWAKKGALIGIVGSIQTRNYENQQGQRVYVTEVIANQFHM
                     LESRGQQSQGNSFQNGNNSNSGNFQNGNNQGYQSPFGNSNPMDISDDDLPF"
     gene            3656..4030
                     /locus_tag="ACHU66_001819"
     CDS             3656..4030
                     /locus_tag="ACHU66_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390084.1"
                     /translation="MATSQTYFYVFSQNNSGGYFVTDENVASEIIIEATEEVLAMARL
                     DEILSQKPEYTNFCPCCGMRWYPEYSDVYLRYWVSDEQYEKFEAERDGHEAMFYPLEG
                     EHRPIPWLRYGMYNYLPSPNFE"
     gene            4044..4253
                     /locus_tag="ACHU66_001820"
     CDS             4044..4253
                     /locus_tag="ACHU66_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390085.1"
                     /translation="MEWVDWVMYQPQTKIDIITKIENDGYTYPRYDKSKNGVKFVMCL
                     ESIKNDCQAVGIRLSEVYPLQTKLF"
     gene            4343..4675
                     /locus_tag="ACHU66_001821"
     CDS             4343..4675
                     /locus_tag="ACHU66_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390086.1"
                     /translation="MQNKIDIPGTSITLEIAGKEIKVKNKIEYDIEMAFKNQDAEPSL
                     DENGDVFEPLFWLSIVAKPQKPIEFHSSLGVKSEKRKATELQKFFEFIENNKQNLFDL
                     CGFRGELE"
     gene            4672..5190
                     /locus_tag="ACHU66_001822"
     CDS             4672..5190
                     /locus_tag="ACHU66_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390087.1"
                     /translation="MSSLTLSLDVSTTGTGWAVFDGSTLIQSGVSKPKQKSFYERAKA
                     IASELKTIQLRAIQEYDKPFEDIVVEQNTVLGPNQQSSIKIGIATGVILGRLLSEEVY
                     FVNVSTWRKYWKFSYKDRSKKSMKQQSVLTVLREFNKQVKDDEADAILVGSYFVNIGK
                     ELGKLESHRNRI"
     gene            5200..5502
                     /locus_tag="ACHU66_001823"
     CDS             5200..5502
                     /locus_tag="ACHU66_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HFR3390088.1"
                     /translation="MDEIKEHAPLLGLICMLFFAGGVATATLFDDNAPQRQPIIIHVV
                     DNAGGMMAGKITDKEIIEGRYTVTAHAYGKFLVTKEQYEAIKVGDPIPDYLKGRKQ"
     gene            5518..5751
                     /locus_tag="ACHU66_001824"
     CDS             5518..5751
                     /locus_tag="ACHU66_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390089.1"
                     /translation="MTVLKNGDGAVKNKFIKITYISDGTEWDLIVNVNEIARLSYGFN
                     ELEFRTPFPNGSNHVSVTQKEFDRLEKLLLEEE"
     gene            5751..5927
                     /locus_tag="ACHU66_001825"
     CDS             5751..5927
                     /locus_tag="ACHU66_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390090.1"
                     /translation="MASEYVRLHIMKHALEHYIKREGASEKDIRQEKKVLDDVVEELE
                     NFKDFINSGCSGGC"
     gene            5927..6196
                     /locus_tag="ACHU66_001826"
     CDS             5927..6196
                     /locus_tag="ACHU66_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390091.1"
                     /translation="MEKLEAIKCSDQKLEIYLTDGDLEAIANGHEVVIPLYTKNRPVK
                     QVSIRPALRQDLLNPLVNFDNKLMSQTDLIAKDFGRQIVADTFKL"
     gene            6193..6459
                     /locus_tag="ACHU66_001827"
     CDS             6193..6459
                     /locus_tag="ACHU66_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390092.1"
                     /translation="MIGEVMKPKKYPYSGRNRLVRKEMPRFVKLGSVALCKKMIDSIE
                     GIRSENSYITVLILKIPKPFLSYEEKTIKVRLPFDEVVSILNQY"
BASE COUNT         2225 a         1115 c         1524 g         1679 t
ORIGIN      
        1 ttatccacag gaggaaaatc atgaaaagat ttgaactacg tgaaaatctt gtctggcaac
       61 gtgctacggc aggagagaag gaaaagctac tggatacagg tctagctgat aaggcaggat
      121 acatccgcct tgtcagagag ctaggtagaa agtatgtggc ctaggaggtg cgacatggac
      181 ggtacttacc catggtttga ctatgaccgt gactatctac aacctgagga accgagacag
      241 gtgcatgatc ctgatgaatg ggtgttcaga ggcggtcaat ggatttatgt aggggatgca
      301 taatgacaga ggaattacta gatacaatcc gacggctgag gtgtgattat ttccacctag
      361 gccgagagct gggcaagatt atcaacgaac aacaggactt gatacttgcc ttgaaacgag
      421 aaaacgaacg cttaaagcgt gagaaatgga atttgaaaca gacgaagagg agaaagaaat
      481 gagtaacaat caattatcaa cccaacaagc aaagcgtgac attgctattg ataccagtgt
      541 ttggactttt caggatgtca aacgctattt tgaccctcaa aacttgctga ctgaaaaaca
      601 agtagggcaa gccttatctc ttatcaaagg acgcaactta aaccctttag caaacgaggt
      661 ctatattgtt gcttacaaga aaaaaactgg aggcacagag ttcagcttga ttgtctcaaa
      721 agaggcattt ctcaagcgtg ctgcccaaaa tccaaactat gagggatttg aggcaggtgt
      781 tgtaaccgtt gatactgatg gagttatgca cgaacgcaaa ggagctctca tgttacctgg
      841 agatactttg gtaggtggtt gggctagagt ctatcggaaa aatttcaaag tacctgtaga
      901 aatctttgtc agtcgtgaag aatatgacaa gaaacagagt acctggaacg ctatgccagc
      961 aaccatgatc aggaagacag ccttagtaaa tgctctacgt gaggctttcc ctgaggattt
     1021 gggaaatatg tacacggaag atgatggtgg tgaaactttt gaccgtatca aacaggcgga
     1081 acctgttgaa agccgtgagg atgtcatggc acgcaaaatg gctcaaattg agcagatgaa
     1141 acaggaacaa gctcagagac agattgacac aagctatcca actgatgatg tgattgatcc
     1201 tgatgatgag ccagcacaag gtgagctatt ggaagatttg gaatactagg aggacaacat
     1261 gcaagaatta caagcaaaag taacgcaggc acaggttgaa atcattgacc gtgaaaagtt
     1321 tgagcaaggt atagccgatg tagtagccaa gtatgaaaat tacacggtca cggccgcaac
     1381 catcaaggat gacaagcaag ttttagctga cctacgcaaa ctcaaaaaac aaatctctga
     1441 tgagcgtatc aagattaagc gtgagttgtc acagtctgct gatgaatttg acaaatatat
     1501 caagggcact agcgagccaa tggataaagt cattgataag attgctactg atgtcaaggc
     1561 ttttgaagaa catcaaaaag ctgtccgcct tgatactgtc aagagctacc tagctaacaa
     1621 agcggcagaa tatatgctgg accctcgcct ctttgatgag aaagcgttgg agtacattaa
     1681 agcctcagac tttatggctg acggtgtgac cttgaagaag gtcacgatga aatcccttga
     1741 ggatatggtc acctttgaat accaaaaaca acaagagcac gaaaaagaaa aagctgctat
     1801 ctctggacaa tgtgctgagt acggtatgac agaccagccg tatatccgta tgttgcagtc
     1861 aatgtctttg gctgatgtaa tgcaacaaat catgtcagac tatctttttg agcaagaaaa
     1921 gcaaaaactg cgacaagctg aggccgaaag ggagcaactt tcggctgagc aacaagcgaa
     1981 acagcaagaa caggttcaaa aatcgccaga aacgcccaaa attgacccag agacaggcga
     2041 gattttgggc ggaggtcaat tataccaccc cgaccaaaaa acgctcagag gggctgaaaa
     2101 tgagcctaaa aagtacactc aaaaaatgac attagaagtc tattttgcca acacagcaga
     2161 aaaggactta ttcaagacag gactgtcaga gcttggtttt gaacacaagc aaaattatca
     2221 ggttagcggt taccaacgga ttcagccagt aactcaagaa caactcaatg aattgtgtgg
     2281 gtggtaataa tggataagca agtaaaacaa gtaattgatg agcttgaacc gtttaatcac
     2341 ggcataacaa tagcaatcca ccaaaacaaa aatgagtgca tagcaacctt tagaatgcct
     2401 agacagtttg acaccaaaaa aatcaaattc actggatgga atgaagatgt taggaacaga
     2461 actagttgcc actctgaaaa tgacttacta gaggcttatg tttacaaggt ttggaatgtc
     2521 tctaatgatt ggatttgcat tgaggtatta ccgttttagg agggatctat ggaaatcaga
     2581 acagtatcag atagcgtaac tatctattcg gacggcaaga ggctacaagt catccatgac
     2641 ttaggggatg agtttgtcct tgattttggg tatcggacgg agaatgtagt caatattgac
     2701 gaacttagtc caggtattgt tggtagcatt gtgccagttt tcaaagtcag cggtttttgc
     2761 tctaaaggtg gagagggtat gcacagctta cgctgggcta tcctccaatt ccaaatattt
     2821 gagaactaca tcaaggacaa tcaggctgat ttgcttgatt ggtataaaaa tccaggaggg
     2881 gaagatggca actaatgaaa ttgaaatcag taatgagcag gcattgatgg gaacacaatt
     2941 acagattggt aaacaggtca tgatggcgtt gcttgaatta catagtgaca gcaagaaagg
     3001 tgggataatt ctacccataa aactaaatga catagatttt aatgtcaaga ttgaaaggga
     3061 ctaaaaatgg caatttttga atttatttta ttagcaggtt ggactttcct atggctatgc
     3121 ctagggttcc tactgggtga gcgcaatgca ggaaaggaca agtcagatga ttaacaatgt
     3181 tgtactggtt ggaagaatga ccaaggacgc tgaacttaga tacacgccat ctaatgtagc
     3241 ggtagcaacg ttcactcttg ctgtcaatcg caaccgtaaa aatgaaaatg gtgagcgtga
     3301 ggctgatttt attaactgtg tcatttggag acaggcagcc gaaaacttag caaactgggc
     3361 taagaaaggt gctctgatcg ggattgtagg tagtatccaa actaggaact acgaaaacca
     3421 acagggtcag cgtgtctatg tgactgaggt tattgctaat cagtttcaca tgctagaaag
     3481 ccgtggacaa cagagccagg gcaactcttt ccaaaatgga aacaactcaa acagtggtaa
     3541 tttccaaaac ggaaacaacc aaggttatca gtctccattt ggtaactcaa accctatgga
     3601 catctctgat gatgacctgc ctttctaaac gataatttat aaaggagaca gacaaatggc
     3661 aacatcacaa acttattttt atgtttttag tcaaaataat tctggtgggt actttgtaac
     3721 ggacgaaaat gtagcgtctg aaatcatcat tgaggctaca gaggaagtac tggctatggc
     3781 acgattggat gaaatcctaa gccaaaaacc agaatataca aacttttgcc cttgttgtgg
     3841 catgcgttgg tatccagaat atagtgacgt gtatctccgt tattgggtga gtgatgaaca
     3901 gtatgaaaag tttgaagcag aaagagacgg acatgaggct atgttctatc ctttagaggg
     3961 agagcataga cctataccgt ggttgaggta tggtatgtat aactatctgc ctagtccaaa
     4021 ctttgaatga gtatgagggt aatatggagt gggtagactg ggtaatgtat cagcctcaaa
     4081 ctaaaatcga tattatcacc aagattgaaa atgacggata cacctaccct cgttatgata
     4141 agtcaaaaaa tggtgtgaaa tttgtaatgt gcctggagtc aatcaaaaat gactgtcagg
     4201 cagtagggat cagactaagc gaggtttatc ctctacaaac aaaactattt taacaggaga
     4261 acaagatgaa acttttagct aaaatcattt tcaactttct acggacagta gacctgatta
     4321 accaaaaagg aggattttac taatgcaaaa taaaattgat atacctggta catcaatcac
     4381 gcttgaaata gcaggtaaag aaatcaaagt caagaataag attgagtatg atattgagat
     4441 ggctttcaaa aatcaggacg cagagccatc tttggatgag aatggtgatg ttttcgaacc
     4501 cctattctgg ctcagtatcg ttgctaagcc tcagaaacct attgagtttc attctagttt
     4561 aggtgtgaaa tcagaaaaac gtaaagctac tgagctacaa aaattctttg agttcattga
     4621 gaacaacaaa cagaatttgt ttgatttgtg tggtttcaga ggagagttgg aatgagtagc
     4681 ttgacattgt cactggatgt ctctactact ggaacaggtt gggctgtttt tgatggctca
     4741 accttaatac agagtggagt gagcaaacca aaacagaaat ctttctatga gcgtgcaaag
     4801 gctatagcca gtgaactcaa gactattcaa ctgagggcaa tccaggaata cgataaacct
     4861 tttgaggata ttgtggtaga acagaatacg gtcctaggtc caaatcaaca atcatctatc
     4921 aaaattggga ttgctactgg cgttatttta gggcgtcttt tgtctgagga agtatatttt
     4981 gtcaacgttt ctacatggag gaaatattgg aaatttagct acaaggatcg cagtaagaaa
     5041 tcaatgaaac aacaatcggt actaactgta ttgagggagt ttaacaaaca agttaaggat
     5101 gatgaggcag atgctatctt ggttggttca tattttgtca acattggcaa agaattgggt
     5161 aagttagaaa gccatagaaa tagaatttga ggttatggaa tggatgaaat aaaagagcac
     5221 gccccacttt tagggctaat ctgtatgctt ttctttgctg gaggtgtcgc aactgctaca
     5281 ctctttgatg ataatgctcc gcaacggcag cctatcatca ttcatgtagt cgataatgca
     5341 ggcggtatga tggcaggaaa aatcacagac aaggaaatca tagagggacg ctacacagtc
     5401 actgcccatg cctacgggaa gtttttggtc acaaaggaac agtatgaggc tatcaaagtc
     5461 ggtgacccaa tccctgatta tttgaaagga cggaaacaat gaataaacag gaagcgattg
     5521 actgtattaa aaaatggaga tggagcagtg aaaaataaat ttataaaaat aacttacata
     5581 tcagacggta ccgagtggga tttaatcgta aatgtaaatg aaatagctag gttatcgtat
     5641 ggttttaatg aactagaatt tagaactcct ttcccaaatg gttcaaacca tgtctctgtt
     5701 actcaaaaag aatttgatag actagagaaa cttttgttgg aggaggaata atggcaagcg
     5761 aatatgtgag attgcatatc atgaagcacg ctttggaaca ttatatcaag cgtgaaggtg
     5821 cttcggagaa ggatattaga caagagaaaa aggtgctgga tgatgttgtt gaagaacttg
     5881 aaaactttaa agactttatc aattctgggt gctcaggagg ttgttaatgg aaaaactgga
     5941 agcaattaag tgtagtgatc aaaaacttga aatctatctt actgatggag atttggaagc
     6001 tatagcaaat ggacacgaag tagtgattcc cctttacaca aagaataggc cagtcaaaca
     6061 agttagtatt agaccggctt taagacaaga ccttctcaat ccgcttgtta attttgataa
     6121 taaacttatg agtcaaaccg atttaatagc taaagatttc gggcgtcaaa ttgttgctga
     6181 tacatttaaa ttatgattgg agaagtaatg aaacctaaga agtatccgta ctcaggaaga
     6241 aatagattgg ttaggaagga aatgccaagg tttgtgaaac ttggctcggt tgcgttatgt
     6301 aaaaaaatga tagatagtat tgagggtatt cgtagtgaga acagctatat tactgtatta
     6361 attctcaaaa tacccaaacc atttttatcc tatgaggaaa aaacaattaa ggtacgcttg
     6421 ccgttcgatg aagtagtaag cattctgaat caatactaaa acaaaaaaag ccaaggcact
     6481 ctctgcccag gctgtggttt tcgctatcaa tattatacca caaaggagac agagagtgaa
     6541 caa
//
LOCUS       DAZQCJ010000103         6523 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000103 DAZQCJ010000000
VERSION     DAZQCJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.104"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            7..324
                     /locus_tag="ACHU66_001828"
     CDS             7..324
                     /locus_tag="ACHU66_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR3390093.1"
                     /translation="MYYPVSSILIEFLVLAIVEKQDSYGYEISQTIKIVADIKESTLY
                     PILKKLEKAGYVTTYTQEFQGRKRKYYTITPAGREHLPFLQGEWNSYKDHIDGIIEGS
                     LRK"
     gene            321..1433
                     /locus_tag="ACHU66_001829"
     CDS             321..1433
                     /locus_tag="ACHU66_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFR3390094.1"
                     /translation="MTRADYLNQLEQALFQLHPSARQEALDYFNEYFDEKDNDEEAII
                     ELGTPDEAAKEIIANLPEDALIPEKDQASPHSSKPFDFNFDFDFQFDWENFFNNFKHS
                     AYQARERIKLTSKIEELPEFSNLQISLEDQALSVQSYEGEAVLLHNPVSEDGSYQAFD
                     YQLVQDSLYISSKPTPNHLYFSNNQISSAILKIPKKLLPLTNLNLKTADSSLTIVDVQ
                     VCEQMVVNAKDSSISMTKVSCPSSALQLEDSTLTISDGQMSELKLEASDAVITLSSMS
                     LSDARITLEDCVWKLKDFVLEKSLNCQAEDSVLTYKPSTDISLDIWEEDSSLKLPKDK
                     AFTMMKEGDITHLSYKQENSPAQLVIHCQDCQLSVG"
     gene            1445..2023
                     /locus_tag="ACHU66_001830"
     CDS             1445..2023
                     /locus_tag="ACHU66_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFR3390095.1"
                     /translation="MNRKEYLQNLEKYLKNLSKEDFQDTLDYFNEYFDEKDNDEEAII
                     ELGTPDEAAKEIIANLYDKERIEDKPNTSNMVWLTILAILAAPIGVPLAITLVALFIT
                     LLLIIFSLFLLLISLWIVLLSLAIAQLLLAFDLLTLSWSSSLLFTGLALVCLALTLLG
                     SKFTLDLGSKTFLLVLHWVQRKIRKGGYYETA"
     gene            2010..2471
                     /locus_tag="ACHU66_001831"
     CDS             2010..2471
                     /locus_tag="ACHU66_001831"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390096.1"
                     /translation="MKQLKPFTKISLSLLGLGAILSLIGYLAGGWTNMKTVAENYFQP
                     QTASFTDIRSIEVNEKLSIMPSPDNQFHLHYYRNQHKDIDLLSHQVQDGKLTISSAYQ
                     GIVTYDGLLEPILNLVNDQNVAYYQPVLQVPKIAQSKHSQGAFKEMSIWIK"
     gene            2468..2863
                     /locus_tag="ACHU66_001832"
     CDS             2468..2863
                     /locus_tag="ACHU66_001832"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFR3390097.1"
                     /translation="MTIQDFQLFVENGDMLIENSHISLIRLLHINGEVAINQSTLTSP
                     ADITDTTRSILTVEDGDFKAENLKLEGKHGISNYDGSIDISLHPKTKTDIHIDASQNN
                     LGIDLPTYSNPQATNYLYISTLDGELNIQ"
     gene            complement(2899..3258)
                     /locus_tag="ACHU66_001833"
     CDS             complement(2899..3258)
                     /locus_tag="ACHU66_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFR3390098.1"
                     /translation="MSIFTTILASIVAIEHLYIMYLETFATHSDSTSRVFNMEKEELQ
                     RKSVTTLFKNQGIYNGLLAVFLIYGIFTGNLEVTTIFVLFVIGAAGYGAATSSPKIIL
                     TQGGPAILALISILLFK"
     gene            complement(3278..3703)
                     /locus_tag="ACHU66_001834"
     CDS             complement(3278..3703)
                     /locus_tag="ACHU66_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR3390099.1"
                     /translation="MQEMEDLLYRLKVADETISNLFEKQLGISLTRYSILQTLLKDAP
                     LHQLALQERLQIDRAAVTRHLKLLEESGYIIRKRNPDNQREVLVWPTEQAREALITNP
                     SAHHQAIKTSMNQILTVEESEQFLATLDKLLIGLQNLPI"
     gene            3864..4622
                     /locus_tag="ACHU66_001835"
     CDS             3864..4622
                     /locus_tag="ACHU66_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFR3390100.1"
                     /translation="MENTPRLNLVIDVLMLAGAILAQSGAEIHRVEDTMIRIAHSHGI
                     EQANVLAIPAAIFFSIDHTNISRMKRIVNSNYDMQKVCDVNQISRSLANEEISLEEAW
                     SQLNAIKQQKPPYNNIQITLAAVVAAPFFTIMFGGSLLDAAGAVLATALAFPISLIVE
                     RYIGIDFVTTFVGAFVFSLTSYLLSATTTLPINPNLINAGAVMPFVPGIAFTNAVRDL
                     MTNHINTGMTKFFQTALIILSLGAGTTVAFMLVR"
     gene            4626..5078
                     /locus_tag="ACHU66_001836"
     CDS             4626..5078
                     /locus_tag="ACHU66_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFR3390101.1"
                     /translation="MTPVHFLLQALASYIAIAAFLIVLNVQRKMLVPGGLLGMLVWLI
                     YLLLLEPTNVLIATFFAAIIGSCVSQIMSIWLKTPSVIFSLAILAPLVPGYRAYMTTT
                     YFVSGDHAQALTNITTVLTLALVIPIGMASGTILLRLYKVLRTGKKAA"
     gene            5135..6373
                     /locus_tag="ACHU66_001837"
     CDS             5135..6373
                     /locus_tag="ACHU66_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFR3390102.1"
                     /translation="MNQNVTNLKLAERGALLSIGAYIVLSGIKLVAGQLFHSDALRAD
                     AFNNISDIIGNIAVLIGLKMAQKPADTDHKFGHWKMEDLASLITSFIMFVVGFQVLYD
                     TLQKLISNSSVEVDIMGAIVGIFSALVMLAVYLYNNRLAKKVRSKALEAAAKDNLSDA
                     VTSIGTSIAIFAAAFNLPIVDKIAAIIITFFILKTAYDIFMESFFTLSDGFDENLLKK
                     YEEDILKLPKIVSVKSQRGRTYGANIYLDVVLEMNPDLSVYESHEVTEQVEQLLTLKH
                     GVFDVDIHVEPSEIPHDEMYEHVYDKLFRFETEIQAHANGYEELIDDQYLLIDAKGHY
                     RNKTQILADHPIQTTYLSNYQMTSISHKSKLVTFEIGEYVHTSLWRRHENWTVIFHQI
                     SKKQASQIEATEKISDLHNP"
BASE COUNT         2003 a         1456 c         1177 g         1887 t
ORIGIN      
        1 gagtttatgt actaccccgt atcctctatc ttgatagaat ttctggtatt agccatcgtg
       61 gaaaagcagg attcctacgg ctatgaaatc agccaaacca tcaagatagt tgctgatata
      121 aaggaatcca cactctaccc tattttgaaa aaattggaaa aggctggcta tgtcaccacc
      181 tatacccaag aatttcaagg gagaaagcgg aaatactata ccatcactcc tgctggacgt
      241 gaacacctcc ccttcctgca gggtgaatgg aatagctaca aagaccatat tgacggaatt
      301 atagaaggga gcttacgaaa atgacacgcg cagattattt gaaccaactg gaacaagcat
      361 tatttcaact tcatccaagt gccagacagg aggctttaga ctactttaat gagtattttg
      421 acgaaaagga taacgacgag gaagctatta tagagctagg aacacctgat gaagcagcaa
      481 aagaaatcat tgccaatcta cctgaagatg ccctaatccc cgagaaagac caagcaagtc
      541 cacattcatc taaaccattt gatttcaact ttgattttga ttttcaattt gactgggaga
      601 attttttcaa caatttcaaa cattcagcct atcaagctag ggaaagaatt aaattgacgt
      661 cgaaaatcga agaattacct gagttttcaa atctacagat ttcgctcgaa gatcaggccc
      721 ttagtgttca aagttatgaa ggagaagctg tcttattgca caatcctgtt tcagaagatg
      781 gtagctacca ggcttttgac tatcaactag tccaagatag tttgtacata tctagcaagc
      841 ctacccctaa tcacctctac ttctcaaaca atcaaatcag ctctgctatt ctcaaaatcc
      901 ctaaaaaact actgcccctt accaatctca atctgaaaac ggcagatagt agtctgacaa
      961 tagtggacgt acaagtatgc gaacagatgg tcgttaatgc aaaagatagc agtatcagca
     1021 tgacgaaagt gagctgccca agctctgctc tacagttaga agactctaca ctaactatct
     1081 cagatggaca gatgagcgag ttaaaattgg aagccagcga tgctgttatc actctctcaa
     1141 gcatgagcct ttcagatgca aggataacat tagaagactg tgtctggaag ttgaaggatt
     1201 ttgtccttga aaagagcctt aactgccagg ctgaagattc tgtcttaacc tataaaccaa
     1261 gtacggacat cagtttggac atctgggagg aagattctag ccttaaactt ccaaaggata
     1321 aggcgtttac aatgatgaaa gagggtgaca tcactcatct cagctacaag caagaaaata
     1381 gccctgctca gctagttatt cattgtcaag attgccagct tagtgtggga taaaggaggt
     1441 ttccatgaat cgcaaggaat atttacagaa tctcgagaaa tatctcaaaa acctatcaaa
     1501 agaagatttt caagatacct tggactattt caatgaatat tttgacgaaa aggataacga
     1561 cgaggaagct attatagagc taggaacacc tgatgaagca gcaaaggaaa tcattgccaa
     1621 tctctatgat aaagagagga ttgaagataa gccaaataca agcaatatgg tctggttgac
     1681 catcctcgct atccttgccg ccccaatcgg cgtcccccta gcaatcacct tggtcgccct
     1741 cttcatcacc cttctcctaa tcatctttag cctctttctg ctccttatct cactctggat
     1801 tgtcctcctc agcctcgcaa tcgcccaact tctcctcgcc ttcgacctcc tcacactctc
     1861 atggtcctcc tccctcctct tcacaggcct ggccctcgtc tgcttggccc tcactctatt
     1921 gggtagtaaa ttcactctag accttggaag caaaaccttc ttgcttgttt tacattgggt
     1981 acaaagaaag atccgcaaag gaggctatta tgaaacagct taaaccattt acgaaaatca
     2041 gtctgagcct gctaggtctt ggagccattc taagcctcat tggctacctg gctggcggtt
     2101 ggacaaatat gaaaactgtt gcagaaaact attttcagcc ccaaacggca agctttacgg
     2161 atattcgttc tatagaggtc aatgaaaaac tgtccattat gccctcgcct gacaaccaat
     2221 tccacctcca ctactatcga aatcagcata aagacattga cctcctcagt caccaagtcc
     2281 aagatggaaa attaaccatt tctagtgcct atcaaggcat cgttacttat gatggtttac
     2341 tcgaacctat cttaaatctt gtcaatgacc agaatgttgc ctactaccaa cctgtcttac
     2401 aggtccctaa aatagcacaa tcgaaacact ctcagggagc attcaaggag atgtctatct
     2461 ggataaagtg actattcaag atttccaact ctttgttgaa aatggtgaca tgttgattga
     2521 aaacagtcac atcagcctta ttaggctatt acacatcaat ggagaagtag caatcaatca
     2581 gtccacatta acaagtccag cggacatcac cgatactacc agaagtattc taactgttga
     2641 ggatggtgat tttaaagcag aaaacctaaa actagaggga aagcatggca tcagtaatta
     2701 cgatggttca atcgacattt ctcttcatcc caaaacaaaa actgacatcc atatagatgc
     2761 cagtcaaaat aatctgggaa ttgaccttcc aacctattcc aatccccaag ccactaatta
     2821 cctctatatc tctacactag atggagaact gaatattcag taattgcaaa aagccctcct
     2881 cacggaaggc tttttaactt atttaaacaa caaaatagaa atcaaggcca agatagctgg
     2941 tccaccttgg gttaagatga tttttggact agaagtcgct gctccgtagc ctgctgctcc
     3001 aataacaaac aagacaaaaa tagttgtcac ttcaaggttg cctgtgaaaa ttccatagat
     3061 aaggaagacg gccaacaaac cattatagat tccctggttt ttaaataggg ttgtcacaga
     3121 cttacgttgt aattcttcct tctccatatt gaatacgcga ctagtagagt ctgaatgggt
     3181 cgcaaaggtt tccaaataca tgatatatag gtgctcaata gctacgatac tagctagaat
     3241 agtggtaaaa atagacataa tgtctcctct caaaaaatta tataggtaga ttttgcaagc
     3301 caatcaacag tttatcaaga gtggctagga attgctcgct ctcctcaaca gttagaatct
     3361 gattcatcga agttttgata gcttggtggt gggcagaagg attcgtgatt agcgcttctc
     3421 tagcctgctc agttggccag acaagtactt ctctctgatt gtcaggattt cgcttgcgaa
     3481 tgatatagcc gctctcttcc aacaatttga gatgacgcgt aaccgctgcg cggtctatct
     3541 gaagccgctc ctgcaaagcc aactgatgca ggggtgcatc ttttagtaat gtttgcaata
     3601 ttgaatagcg tgtcaaactg attcccaact gcttttcaaa aagattggaa atcgtctcat
     3661 ctgccacttt caatctgtat aacaaatctt ccatttcttg cattatctac ctcttttgat
     3721 tgattaatca atcattgata agtcaattat actataatcc gaacaatttt caactatttt
     3781 gcctctctat aaaaaaatat gttgcctaca cctatatttt gttataatta agaaaataac
     3841 tagcagaggg atgggactta cttatggaaa acactcctag actaaacttg gtcatagacg
     3901 tgctcatgct cgctggtgcc attttggcac aaagcggtgc agaaattcac cgagttgaag
     3961 ataccatgat tcgtattgca cattcacacg gaattgaaca ggctaatgtc cttgccatcc
     4021 ctgctgctat ctttttctcc atcgaccata cgaatatttc gcgtatgaaa cgcattgtca
     4081 attcaaatta cgacatgcag aaagtttgtg acgtcaacca aatctcccgt tccctagcca
     4141 atgaagaaat cagcctagaa gaagcttgga gccaattaaa tgcaatcaaa caacaaaagc
     4201 caccttataa caatatccaa ataaccttag ctgccgtagt agccgctcca ttttttacca
     4261 tcatgttcgg aggttctctt ttagatgcag caggtgctgt cctcgcaaca gctttagctt
     4321 tccctatctc actaattgtc gagcgctata ttggaattga ttttgtcacc accttcgttg
     4381 gcgcctttgt cttctcactc acctcttatt tattaagcgc aaccactact ctccccatta
     4441 atcctaacct gattaatgcg ggggcagtca tgccttttgt accagggatt gcttttacaa
     4501 atgctgtaag agacctcatg accaatcata tcaatacagg gatgaccaaa tttttccaaa
     4561 ctgcgcttat tatcttatcg ctcggtgcag gtacaactgt ggcctttatg cttgtgaggt
     4621 aatctatgac accagttcac ttcttattac aggcactcgc ctcttatatc gctatcgcag
     4681 ccttcttaat tgttctcaat gttcaaagaa aaatgctggt ccctggtgga ctcctaggca
     4741 tgcttgtttg gcttatttat cttttactat tggaaccaac aaatgtcttg attgctacct
     4801 ttttcgcggc aattatcgga tcctgtgtca gtcaaatcat gagtatttgg ttaaaaacac
     4861 cctccgtcat cttttcattg gctatacttg caccacttgt tccaggctat cgagcctaca
     4921 tgaccacgac ttatttcgtt tctggagacc acgctcaagc cctaaccaat atcactaccg
     4981 tcttaacttt agccttagtc attcccatcg gtatggccag cggaacaata ttattacgcc
     5041 tttataaggt tcttcgaacc ggcaaaaaag cagcctaacc ccttgaaaat aaggggcctt
     5101 tttgttaaaa tgaaaactta tcaaaggaga tgctatgaac caaaacgtaa ccaatctaaa
     5161 acttgctgaa cgtggtgcct tgctatctat cggtgcctat attgtcttat caggtatcaa
     5221 actcgttgca ggacagctct ttcattcaga tgccttacga gcagatgcct tcaataatat
     5281 ttctgatatt atcggtaata tcgctgtact tattggacta aaaatggcac agaaaccagc
     5341 tgataccgat cataaatttg gacactggaa aatggaagac ttggcaagct tgattacatc
     5401 cttcatcatg tttgtcgttg gtttccaagt actctatgat accctccaaa agctaatcag
     5461 caattcttct gtcgaagtag acattatggg ggctattgtc ggaatttttt ctgcactagt
     5521 tatgttagct gtttatttat ataataaccg cttggcaaaa aaagtacgtt ccaaggcctt
     5581 agaagcagct gccaaggata atctatccga tgcagtcact tccataggaa cttctatcgc
     5641 gatctttgct gcggctttca acctccctat tgtcgataaa attgcagcca tcatcattac
     5701 cttctttatc ctaaaaacag cctacgatat ttttatggaa agcttcttta ccctatctga
     5761 tggatttgat gaaaacttat tgaaaaagta cgaggaagat attctcaaac tcccaaaaat
     5821 cgtttctgtc aaatcacaac gtggtcggac ttatggagcc aatatctacc ttgatgttgt
     5881 tttagaaatg aatccagact tatccgttta tgaaagtcat gaagtgactg agcaggttga
     5941 gcaactccta acactcaaac atggagtttt tgatgtggat attcacgtag aaccatctga
     6001 aattccacac gatgagatgt atgagcatgt gtacgacaaa ctcttccgtt ttgaaacaga
     6061 gatacaagca catgctaatg ggtatgaaga attgattgac gaccagtatc tcttaattga
     6121 tgccaaaggc cactaccgaa ataaaactca gatacttgcc gaccacccca tccaaacgac
     6181 gtatttatca aattaccaaa tgacatctat tagccataaa tcaaaattag ttacgtttga
     6241 aattggcgaa tatgttcata cgtcactttg gcgccgtcat gaaaattgga ctgttatttt
     6301 ccatcaaatt tcaaagaaac aagcctctca gatcgaagct actgaaaaaa tctctgattt
     6361 gcataatcca taataaaaaa taggaatttt ccctggatta tagggagaat tcctattttt
     6421 taaccttccc tttgagctat tatcagtctt ttatactcaa tttgtagtta tttagttttt
     6481 gtttagtata tgataaaagc taaataacta tagaagatta gag
//
LOCUS       DAZQCJ010000104         6285 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000104 DAZQCJ010000000
VERSION     DAZQCJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.105"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(78..1070)
                     /gene="asnA"
                     /locus_tag="ACHU66_001838"
     CDS             complement(78..1070)
                     /gene="asnA"
                     /locus_tag="ACHU66_001838"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR3390103.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEIVEVQGPILSRVGDGM
                     QDNLSGVENAVTVNVKLIPDATYEVVHSLAKWKRHTLARFGFHEGEGLFVHMKALRPD
                     EDSLDPIHSVYVDQWDWEKVIPDGRRNLAYLKETVEQIYKAIRLTELAVEARYDIESV
                     LPKKITFIHTEDLVKNFPDLTPKERENVVAKEYGAVFLIGIGGELADGKPHDGRAPDY
                     DDWTSPSEAGYKGLNGDILVWNEALGSAFELSSMGIRVDEEALKRQVAITGDEDRLEF
                     DWHRALLNGLFPLSIGGGIGQSRLAMFLLRKKHIGEVQSSVWPQEVRDNFENIL"
     gene            complement(1155..2204)
                     /locus_tag="ACHU66_001839"
     CDS             complement(1155..2204)
                     /locus_tag="ACHU66_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR3390104.1"
                     /translation="MSRQIAHLPSLIPFGFILADNRYTYREVFMEGQFEAVVEVDEAG
                     QLSSYVWDCEMEEVYTAHLVTAPAGAFVGQVREAYQSILARVEEVCCIALPFSKDQSN
                     RIAQLIKEKWGDLPDYPFAKLPTYGAFRHPSNNKWYALVSQIPRDKLDGSGSKEEVEI
                     VNLKVDGREIAELLSQSGIFPAYHMSKKSWVSVLLDETVEDQVVFALLEKSRYLVGPK
                     SYKAEQGSDYWVIPANPKVYDIDTEFAENKVVYWPQKSTIQAGDIVAIYVTAPVQAIR
                     YVCRVLGANLENHGESDIPTGKKLMQVELLAQFSDDVLQRARMMDLGVRAVRGPRRLT
                     EGVIEVLTSEVKNLH"
     gene            2279..2671
                     /locus_tag="ACHU66_001840"
     CDS             2279..2671
                     /locus_tag="ACHU66_001840"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HFR3390105.1"
                     /translation="MSKKIISVVAAAIEKDGKIFCAQRPEGKSLGGYWEFPGGKLEAN
                     ETPEEALVREIKEEFDSEIEIVSFINEASYEYDFGIVTMKTYLSKLVTGDLTLLEHQD
                     SKWLPIEDLETLEWAPVDIPAVKCLQQK"
     gene            complement(2695..5568)
                     /locus_tag="ACHU66_001841"
     CDS             complement(2695..5568)
                     /locus_tag="ACHU66_001841"
                     /EC_number="3.1.4.4"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004630 - phospholipase D activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR3390106.1"
                     /translation="MSNNNLFQDQLLHSLQYGFIDREIFQEGVYSPKILINDTETHRY
                     VLNDIQEELSKAQSFAFSVAFITQSGLALIKSQLSDLADEGIQGRILISPYLDFNDPI
                     AMKELLKLKNVQVRITPVNLQMHAKFYLFEQKDKQVIISGSSNLTHTALKINYEWNVK
                     LTSTHNGDFIQNAKEEFDRIWEKSIVLDLEAINAYSARRQTVIKTAMIRDESIASYQE
                     TIRPNKMQEQALEGLATVRKNGAQKALVISATGTGKTYLSAFDVKQYNPDRMLFIVHR
                     EQILQKSLKDFQKVLGFEDDEGCIYKSGMDISQKRYIFATIQTISRADNLQAFDPTFF
                     NYILIDEVHKAGADSYKKVMNYFQPDFYLGMTATPERTDGQNIYELFDYNIAYEIRLQ
                     EALDNDMLCPFIYYGVSDIKIDGQLIDEKATFSNLVSEQRVNHILEKIAYYGVSGEAV
                     KGLIFCSSVNEAKELEGQFNARGLRTRALSGEHSQKERQRVVQDLENGNLDYILTVDI
                     FNEGIDIPSVNQVVMLRNTQSSIIFIQQLGRGLRKHDSKEYVTIIDFIGNYQNNYLIP
                     VALFGDQSMNKDNYRREVREPNILKGLTTINFEKVAKEQIFSSITNTTLSSIKILRDA
                     FKDLENKLGRVPYLSDFIKLNSIDPLVFFENSSFQNYGDVINKFSEETILLSEVEKRF
                     LNFITFEILNGKRKHELYLLKLLVENEGLVSSDKWFECIIEHDLDFQTDVLDSVTRVL
                     DLSFLKAQEQKKYGTDALVTFDGELYRLNEEIQESLVNNTKFTQLFLDVIETGILKSK
                     DYPEIFTLGQKYTRREVLKLMNVAKDEPPLNIGGYKIDKDTNSCPIFITYHKSEDISD
                     TIKYDDELLNESTLKWFSKNKRTLDSNDVSTITQSNENGLRLELFVIKDDSEGGEFYY
                     LGPLSYVENSASEVMKNGDSVVQMLFKLDSPVETNLYKYLTDK"
     gene            complement(5653..>6285)
                     /locus_tag="ACHU66_001842"
     CDS             complement(5653..>6285)
                     /locus_tag="ACHU66_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390107.1"
                     /translation="NFGNEYVSCKCVSLLFAVADWIKQKTNEVERKDSFVKSQTNESI
                     VDLINRYQELKKFEDEIANWKRRDIILGTDIPVSGVRDDYGEYSYLYPILDMFEYWRD
                     GNYGQLSKLLEDAKNMNTSNGKWAGQIREIFNEKKYNSFEFITIDEKTYAKSEITVKV
                     EWEQDSSIRTESLRFNCVYTGLKEEDNLVAPWRENGEWRLIPLDVEKIFY"
BASE COUNT         1863 a         1378 c         1069 g         1975 t
ORIGIN      
        1 aaatcacaga taatcttgtt gtaaatgttt tcagatagca gattgaaagc agaagaaaag
       61 agattgaagc caatctctta taaaatattc tcaaaattat cgcgcacttc ttgtggccaa
      121 acgctggact gcacttcacc aatatgtttc ttacgaagca agaacatggc cagacgggat
      181 tgaccgatac cgccaccgat agacagcggg aagagaccat tcaaaagggc acgatgccaa
      241 tcaaattcca aacggtcctc gtcacctgta atagcaacct ggcgcttaag cgcttcctcg
      301 tctacacgaa tccccatcga ggacaactca aaggctgaac caagtgcttc gttccaaacc
      361 aagatgtctc cattgagtcc tttgtagcct gcttctgatg ggcttgtcca gtcatcatag
      421 tctggtgcac gtccatcatg tggtttccca tctgccagtt ctccaccaat accgattagg
      481 aaaaccgcac cgtattcttt ggcaaccaca ttttcacgct cttttggtgt caagtctggg
      541 aagttcttca ccaagtcctc tgtgtgaata aaggtgattt tctttggtaa aacagactca
      601 atgtcataac gagcttcaac ggccaattcg gtcagacgga tagccttgta aatctgctct
      661 actgtttctt tcaagtaagc taaattccga cgaccatcag ggatgacctt ttcccagtcc
      721 cactgatcca catacaccga gtgaatcgga tccaaggaat cctcatctgg acgcaaggct
      781 ttcatatgta caaaaagacc ttctccttcg tgaaatccaa agcgagccaa ggtatgacgc
      841 ttccattttg caagcgaatg aaccacttca taagtagcat ctggtatcag cttcacatta
      901 acggtaactg cattctccac accggacaaa ttatcctgca taccatcccc gacacgactc
      961 aaaatcggcc cttgcacttc tacgatttct agcttgtctt tcaaatactg agtgaatgta
     1021 tttttgacga aagaaatctc ctcttgctga tgaatgaagc ttttcttcat accttacctc
     1081 ctcgtaaatt tatacacctt attatactct tttttaccca cttgtaaagt ctttttatta
     1141 aatattctga taatttaatg gaggtttttc acttcggatg tcagtacttc tatcacgccc
     1201 tctgtcaagc gtctaggacc acgtaccgcc ctgactccca agtccatcat gcgcgctctc
     1261 tgcaaaacat catcggaaaa ctgagcaagc aattccacct gcatgagttt ctttcctgtt
     1321 ggaatatctg actctccatg attttccaag tttgccccca agactcgaca aacatagcga
     1381 atggcctgta caggtgccgt gacataaatg gcaacgatgt ccccggcttg aatggttgat
     1441 ttttgtggcc aatagactac cttattttcc gcaaattcgg tatcgatatc atagaccttg
     1501 ggattggctg gaataaccca gtaatcagaa ccttgctccg ccttgtagga ttttggtcct
     1561 acaagatagc ggctcttctc aaggagggca aacaccacct ggtcctccac agtctcatcc
     1621 agcaagaccg acacccaaga cttcttagac atgtgatagg ctgggaaaat gcccgactga
     1681 cttagcagct ctgctatttc ccgaccatcg accttgagat tgacaatctc cacctcttct
     1741 ttagacccgc tcccgtccag cttgtccctc ggaatctgac tgaccagggc gtaccactta
     1801 ttattggagg ggtggcgaaa agccccatag gtcggcaact tggcaaaggg atagtcagga
     1861 aggtcccccc acttctcctt gatgagctgg gctatgcggt tgctttggtc tttggaaaat
     1921 ggcagagcga tacagcagac ttcctctacc cgagccaaaa tggactgata agcctcacgc
     1981 acctgcccca caaaagcccc tgctggcgca gtcaccagat gggcggtata gacctcctcc
     2041 atctcacaat cccagacata ggacgacagc tgaccagcct catcaacctc caccaccgcc
     2101 tcaaactgcc cctccatgaa gacctcacgg taggtgtaac gattgtcggc gagaataaaa
     2161 ccgaaaggga tgagggaggg gaggtgggca atttgtctag acattttaac tcctttaaag
     2221 taatatatag agaaagtata acaaaatctg taaaagaatt tattgtggag gtcaaatctt
     2281 gtcaaaaaaa attattagtg tggtagctgc agcaattgaa aaagatggaa aaatattttg
     2341 cgctcagcgc cctgaaggaa agagtttagg agggtattgg gaatttccag gaggaaaact
     2401 cgaagccaat gaaactcctg aagaagcctt agttcgtgaa atcaaagaag aatttgattc
     2461 cgaaatcgaa atagtatctt tcatcaacga agcatcttat gaatatgatt ttggtatcgt
     2521 gacaatgaaa acctaccttt caaaattagt cacgggtgat ttaactttat tagaacacca
     2581 ggattctaaa tggctcccca tcgaagattt agagacatta gaatgggctc cagtagatat
     2641 tcctgccgtc aaatgtttgc aacaaaaata gagattcgct ggaatctcta ttttttattt
     2701 atctgtcaaa tacttataca agtttgtctc cactggtgaa tccaacttaa ataacatttg
     2761 gacaactgaa tctccatttt tcattacttc acttgcacta ttttcaacgt atgatagtgg
     2821 accaagataa taaaattctc caccttctga atcatctttt atgacaaaaa gttctaacct
     2881 caatccattt tcattagatt gagtaatcgt cgatacatca ttagaatcta atgtacgttt
     2941 gttctttgaa aaccacttca aagttgactc atttagaagt tcgtcatcgt attttatggt
     3001 atcactaata tcctcacttt tatggtaagt gatgaaaatc ggacaagaat tggtgtcctt
     3061 atcaattttg tatccaccaa tatttaaagg tggttcgtct tttgcgacat tcattaattt
     3121 aagcacttca cgtcgagtat atttctgacc aagtgtgaaa atttcaggat aatctttcga
     3181 ttttagaatc cctgtttcaa tgacatccaa aaataattga gtgaatttgg tattattcac
     3241 caaagattct tggatttctt cattcaaccg atacagttca ccgtcaaaag taaccagtgc
     3301 atccgtacca tatttctttt gctcctgggc ttttagaaat gataggtcca acactcttgt
     3361 taccgaatct aaaacatctg tttgaaaatc taaatcatgc tcaataatac attcaaacca
     3421 tttatctgac gaaactagtc cctcattttc gacaagcaac ttcaacaaat acagttcatg
     3481 ttttcgtttg ccattcaaga tttcaaatgt aataaaattc aaaaatcgct tttctacttc
     3541 tgatagaagg attgtttctt ctgaaaattt attgatgaca tctccgtaat tttgaaaact
     3601 agagttttca aaaaagacaa gcggatcaat gctgtttaat tttatgaaat ctgatagata
     3661 cggaactcta cccaacttat tctctaagtc tttaaaagca tctcgtaaaa ttttgattga
     3721 tgagagtgtc gtatttgtga tagaagaaaa aatttgttct ttggcaacct tctcaaagtt
     3781 gattgtcgtc aatcctttta aaatgttggg ctctctgacc tcacgacgat aattgtcttt
     3841 gttcatagac tgatcgccaa atagtgcaac aggaattaga tagttgtttt gatagtttcc
     3901 aatgaaatca ataatcgtaa catattcttt cgaatcgtgc tttcgcaagc ctcgacctaa
     3961 ttgttgaata aaaatgatac tcgattgggt attgcgaagc atgacaacct ggttgacact
     4021 cggaatatca attccctcat taaaaatatc aaccgtcaga atatagtcta agtttccatt
     4081 ttctaagtct tgaactactc tctgtcgttc cttttggcta tgttccccag aaagagcgcg
     4141 tgtacggagt cctcttgcat taaactgccc ctctagctct tttgcttcat ttactgaaga
     4201 acagaaaatc aatcctttta ccgcctcacc acttactcca tagtaagcaa ttttttctaa
     4261 aatatgattg acacgttgtt cagaaactaa atttgaaaaa gtggcttttt catcaattaa
     4321 ttggccatca atcttaatgt ctgaaacacc gtagtatata aatggacaaa gcatatcatt
     4381 atcaagtgcc tcttgtaaac ggatctcata ggcaatatta tagtcaaaaa gctcataaat
     4441 attttgtcca tctgttcgct ctggtgttgc tgtcatccct aaataaaaat ctggctgaaa
     4501 ataattcatc acttttttat aggagtctgc acctgcttta tgaacctcat caatcaaaat
     4561 atagttaaag aaagttgggt caaaagcttg aaggttatct gctctcgaaa tcgtctgaat
     4621 agtcgcaaag atatacctct tctgtgatat atccatccca ctcttataaa tacagccctc
     4681 atcatcctca aatcctaaca ccttttgaaa gtcttttaat gatttttgta ggatttgttc
     4741 tcggtggact ataaataaca ttcgatctgg attgtattgt ttgacatcaa aggcagaaag
     4801 ataggtttta cctgttccag ttgcactaat gaccagagct ttttgtgcac catttttcct
     4861 aacggttgcg agaccttcta gagcttgctc ttgcatcttg ttgggacgaa tggtttcttg
     4921 gtaagatgct attgactcgt cccgaatcat cgctgttttt atcaccgtct gcctacgagc
     4981 tgaatatgca ttgattgctt ctaggtctaa aacaattgat ttttcccaaa tacgatcaaa
     5041 ctcttctttt gcattctgga taaaatcacc gttatgtgtt gaagtcaatt tcacattcca
     5101 ctcatagtta attttcaagg ctgtatgagt taaatttgaa ctaccagaaa tgataacctg
     5161 tttatctttt tgctcaaaca gataaaactt tgcatgcatt tgcaaattga ctggtgttat
     5221 ccgtacttgg acatttttca gtttaagcaa ttctttcatc gctatgggat cgttgaaatc
     5281 cagatacgga gaaatcaaaa ttctcccttg gatcccctca tccgccaaat ctgataattg
     5341 acttttaatt agggctagtc cagactgagt aataaaagcc acactgaagg caaaagattg
     5401 ggccttgctt aactcttctt gaatatcatt taaaacatag cgatgagtct ctgtatcatt
     5461 tattaaaatt ttaggcgaat agaccccttc ttgaaaaatc tctctatcaa tgaaaccata
     5521 ttgcaaagaa tgaagaagct ggtcttggaa taagttgttg ttggacatga taggtttcct
     5581 ttatctctat atagaattat tatctgtaaa attcagaaaa ttgtaaagtc aggaaacaaa
     5641 ttatttaata acttagtaga aaattttctc cacatccaag ggaatgagcc tccactctcc
     5701 attttccctc cacggtgcaa ccaaattatc ctcctcttta agaccagtat aaacgcagtt
     5761 gaatctcaaa gattcggttc ttatcgaact atcttgttcc cattctactt tcacagtaat
     5821 ctctgacttt gcatatgttt tttcatcaat cgttatgaat tcaaatgaat tatatttctt
     5881 ttcattaaat atttctctta tttgacctgc ccattttcca ttggaagtat tcatattttt
     5941 tgcatcttct aataacttcg aaagttgtcc ataatttcca tctctccagt attcgaacat
     6001 atctaatata gggtataaat atgaatactc gccataatca tctcttacac cacttacggg
     6061 aatatcagta ccgagaatta tatcccgtct tttccaattt gctatttcat cctcaaactt
     6121 ctttagttcc tgatatctat taatcaaatc cacaatagat tcgtttgttt gtgatttaac
     6181 aaaactatct tttctctcaa cctcatttgt tttttgcttt atccaatcag cgacagcaaa
     6241 aagtaatgaa acacacttgc aagatacata ctcattacca aaatt
//
LOCUS       DAZQCJ010000105         6228 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000105 DAZQCJ010000000
VERSION     DAZQCJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.106"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(33..584)
                     /locus_tag="ACHU66_001843"
     CDS             complement(33..584)
                     /locus_tag="ACHU66_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002268947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFR3390108.1"
                     /translation="MTKALISIDYTIDFVADEGKLIAGKSAQAISERIAQVTQEAFEN
                     GDYIFFAIDGHEEGDEFHPEAQLFPSHNIIGTQGRDLYGPLADFYQKHKGHARVRWMD
                     KRHYSAFSGTDLDVRLRERGVDTVVLTGVLSDICVLHTAIDAYNKGYRIEVVSSAIAA
                     LTEENHQFALNHLRHVLGATIID"
     gene            complement(586..1374)
                     /gene="codY"
                     /locus_tag="ACHU66_001844"
     CDS             complement(586..1374)
                     /gene="codY"
                     /locus_tag="ACHU66_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFR3390109.1"
                     /translation="MTTLLEKTRNITSILKRSEEQLAEELPYNAIAEHLSAIIDCNSC
                     IINSEGEVLGYHMSYETNNDRVEEFFQNKQFPEGYVKAVAQVYDTQVNLPVESELTAI
                     PVESRSTYPNGLTTIAPIHVTGIRFGSLIIWRNDEQFHDDDLILVEIAATVVGIQLLN
                     FQREEDEKNIRRRAAVNMAVNTLSYSEMKAVAAILGELAGNEGQLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLIPAIFDEIKKRDY"
     gene            complement(1845..3059)
                     /locus_tag="ACHU66_001845"
     CDS             complement(1845..3059)
                     /locus_tag="ACHU66_001845"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFR3390110.1"
                     /translation="MKQFNKSTKLDDVAYDIRGPVLEEAMRMRANGEQILRLNTGNPA
                     EFGFTAPDEVIRDLIHNARKSEGYSNSKGIFSARKAIMQYCQLKKFPNVDIEDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGKAVHYVCDEAAEWYPDLAD
                     MESKVTSRTKAIVLINPNNPTGALYPKEILKGIIDIARRHELIIFSDEIYDRMVFDGA
                     VHIPIATLAPDLFVVTMNGLSKSHRICGFRVGWMVLSGPKKHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGYQSVDELLMPGGRLYEQREFITKAINDIPGLSAVKPKAGLYV
                     FPKIDREMYRVEDDEQFVLDFLKQEKVLLVHGRGFNWKDPDHFRIVYLPRVDELAEIQ
                     EKMSRFLRQYRR"
     gene            3214..3666
                     /locus_tag="ACHU66_001846"
     CDS             3214..3666
                     /locus_tag="ACHU66_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFR3390111.1"
                     /translation="MTQSYKTILVAVDGSKGAELALHKAIHVALRNQARLIIAHVIDT
                     RALHNVVAFDASVYESLEREAELLLEEYKQEALNAGLTDVQICIEFGNPKTLLAIDIP
                     KETGADLMLLGATGLNAFERLLIGSSSEYIMRHATIDLLIVRDGEKSL"
     gene            complement(3682..5047)
                     /locus_tag="ACHU66_001847"
                     /pseudo
     CDS             complement(3682..5047)
                     /locus_tag="ACHU66_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002891795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
     gene            5117..6076
                     /locus_tag="ACHU66_001848"
     CDS             5117..6076
                     /locus_tag="ACHU66_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR3390112.1"
                     /translation="MKKILVLHTGGTISMQANHQGEVASSKINPMTQIDSPLEEIQVT
                     SLDFLNVPSPHIRLEHMMTLYQKIKEEQMNFDGFVITHGTDTLEETAYFLDTMAIPEK
                     PIVLTGAMRSSNELGSDGIYNYRTALRVAADDKSADKGVLVVMNDEIHAAKYVTKTHT
                     TNVSTFQTPTHGPLGLVTKREILYFKTAEPRVRFDLSTVTGTVPIIKAYADMDSILLD
                     SLTASSISGLVIEALGAGNLPPTVLPAIKKLLDQKIPIILVSRCFNGIAEPVYAYQGG
                     GIQLEQDGILFVKELNAQKARLKLLIALNAGLEEQSLADYIQG"
BASE COUNT         1964 a         1488 c         1024 g         1752 t
ORIGIN      
        1 agtcgagttc tttcccgctc cccactatac tattagtcaa taattgtcgc accgagtaca
       61 tgacgcaaat ggttaagagc aaactgatga ttctcctccg tcaatgcagc aatggctgat
      121 gagaccacct caatacgata ccccttatta taagcatcaa tagctgtatg aagaacacaa
      181 atatcagaca aaacacccgt caagacaacc gtatctacac cacgctctct tagtcgaaca
      241 tccaaatccg tcccagaaaa agcagaataa tgacgtttat ccatccaacg aacacgcgca
      301 tgccctttat gtttttgata aaaatctgct aaaggaccat acaaatcacg tccttgcgtc
      361 ccaatgatat tatgacttgg gaaaagctga gcttcaggat gaaattcatc cccctcctca
      421 tgaccatcaa tcgcaaagaa aatatagtct ccattttcga aagcttcttg cgtaacctga
      481 gcaattcgtt cagaaatagc ctgagctgat tttccagcaa tcaactttcc ttcatctgcc
      541 acaaaatcta ttgtataatc aatcgaaatc aatgcttttg tcattttagt agtcacgttt
      601 cttaatttca tcaaaaatag ctgggatgag aactttcaaa taagtcccct tcattcccaa
      661 agaacgactt tcaataatcc ctgcactctc caacttacgc agtgcattca caatcaccga
      721 gcgtgtaata ccgatacgat ctgcaatcac agaagcagtc aattgcccct cattgccagc
      781 caattcaccc aaaatagctg caactgcttt catttctgag taagatagtg tatttaccgc
      841 catattaact gccgcacgac gacggatatt cttctcgtct tcttcccgtt ggaaattaag
      901 taactgaata ccaactactg ttgccgcaat ctcaaccaaa atcaaatcat catcgtgaaa
      961 ctgctcatca ttccgccaaa taataagcga accaaaacga atccccgtta cgtggatagg
     1021 cgctatcgtt gtcagcccgt ttggataagt cgagcgtgat tcgacaggga tggcagtcaa
     1081 ctcgctctcg acaggcaaat taacctgcgt atcgtaaacc tgcgcaactg cttttacata
     1141 tccttctggg aattgtttgt tttggaaaaa ttcttccaca cgatcattgt ttgtctcata
     1201 actcatgtgg tatcccaaaa cttcaccttc gctattaata atacaagaat tgcaatcaat
     1261 aatagctgat agatgttcag caatagcatt gtaaggcaat tcttctgcca attgctcttc
     1321 ggaacgcttc aaaatagaag taatattccg tgtcttctct aataatgttg tcatattttc
     1381 ctctttactc tgtaattgct ttcagtatag caaaaaatac ataaaatttc aacaattatc
     1441 agaatattta ccgttgttag aaaaacacaa acaatttcat ccaagcaaaa agaaataggc
     1501 tgggcaaaaa gcccagcacc gcttctcaaa gttagtgtca acatctcagc gcagtggttg
     1561 attgggttta acagtccagt ggactgttaa aggttggaga taagatttgc gaagcaaatc
     1621 tcagcaattc gcattttaca ctccaaatct ggcctatacg actgatgcga acagagttcg
     1681 cttcatctcc accctcaaac tgtctcccag acagtttgag ctgtgcgggg gtgggagtga
     1741 aacagtctgg gaatagactg tttcagctca acaactagaa ataaagactt gttgacgaac
     1801 tcttcttatg aatggttgag ttctttccca ctcccaaaat tccattatct acgatattgc
     1861 cgcaagaatc gtgacatttt ttcttgaatt tctgccaact catcaacacg tggtaaatac
     1921 acaatacgga aatgatctgg atccttccag ttaaaacctc gaccatggac caaaagtact
     1981 ttttcttgct tgaggaaatc caatacaaat tgttcatcat cctcaactct gtacatctca
     2041 cgatcaattt ttgggaatac atacagacca gccttaggtt ttacagcaga aagtcccgga
     2101 atatcattaa tcgccttggt aatgaactcc ctttgttcat ataagcgtcc gccaggcatc
     2161 aagagctcat caaccgattg gtaacctccc aaagaagttt gaacaacctg ttgagccaaa
     2221 acgtttgaac acaaacgcat attggacaac atattcaagc cttcaatgta accttttaca
     2281 tgctttttcg gaccagataa gaccatccaa cctactcgga aaccacaaat acgatgtgat
     2341 ttagacagtc cattcatcgt cacaacaaat aaatctggag ccagtgtcgc aatagggata
     2401 tgaacagctc catcaaagac catacgatca taaatctcat cagagaaaat gatcaattca
     2461 tgtctacgag caatatcaat gattcctttt aaaatttctt tcggatagag agcaccagtt
     2521 ggattatttg gattgataag gacaattgcc ttggttcgag aagtcacctt ggactccata
     2581 tctgccaaat ctggatacca ttcagctgct tcatcacaaa cataatgaac agctttacca
     2641 ccagccaaac taactgcggc agtccagaga ggatagtctg gcattggcac caatacctca
     2701 tcaccattat ctagcaaacc ttgcatagac ataacaatca gctcactcac gccgtttcca
     2761 agataaatat cttcaatgtc aacatttgga aatttcttta gctgacaata ctgcatgata
     2821 gccttacgtg cagaaaaaat tcccttactg ttcgaatatc cctctgattt acgggcgtta
     2881 tgaatcaagt cacgaataac ttcatctgga gcagtaaaac caaattctgc aggattcccc
     2941 gtattcaaac gtaaaatctg ctctccattg gcacgcatgc gcatggcctc ttccaaaaca
     3001 ggcccacgaa tatcatatgc tacatcatcc aattttgttg acttgttaaa ttgcttcatg
     3061 tcgtcacctc gactttctat cttttctatt gtactgtaaa agctaaaaaa gtacaagaat
     3121 cctttacatt ttctctcaaa aggcttataa taaaaatgta aggaactact ctacctattt
     3181 cgggacaaag ctcttctgaa aggagtgatt attatgacac aatcttacaa aacaatactc
     3241 gtcgctgtag acggctctaa aggagcagaa ttagctcttc ataaagccat tcatgtcgca
     3301 ctacgcaacc aagcacgtct gattatcgcc catgtgattg acacccgtgc tctccacaat
     3361 gttgttgcat ttgacgcctc tgtctatgaa tctttagaaa gagaggcgga gctcctcctc
     3421 gaagaataca aacaagaagc tctcaacgcc ggtctgaccg atgtacagat ttgcattgaa
     3481 ttcggtaacc ctaaaaccct actggctatc gatattccaa aagaaaccgg tgctgatctt
     3541 atgttacttg gagcaactgg tctaaacgcc tttgaacgtc tcctgatcgg atcctcatcc
     3601 gaatatatca tgcgccatgc aactattgat ttactcattg ttcgcgatgg agaaaaaagt
     3661 ttataaacca aaagacttga tttacgaatc aagtcttttc atctgtcttt tcagctcttc
     3721 ctgaatcttt tcctccggcg caaaccgttc ttcccaatca tcaggtttta aaattttatg
     3781 ggtaattgga tcaaaatggg ccttcccatc tggaaaagtt ttccccatat ttgcagtatg
     3841 gactatctga aaaattggtt ctggatcaac ccccatcaaa acaaacgaac catatgtaaa
     3901 ataaagaata tccagcaaag catctacctg atcctgcatc gaaaccccga aacctctctt
     3961 gccagatacc ttagcagcag cagtatctaa atcccgatga agttgttgaa taaaatcatg
     4021 aaactcaact tcagattctg aagctgcatg tagaaattct accaactcct ccactttaaa
     4081 accagcgcga tgcaaagccg tctgtccatc atacacactg ggaagttctt gtgtttcacc
     4141 atccattaaa aaatgaaaat cctttaccct attaaaatta tcgtccttgc tctgaaacat
     4201 cctatccctc aatcacacca aaatgaatta atgccttata aatcccatca cgattatttg
     4261 tatccgtaac aaaggaagca gcttgtttta cctttttcgt cccatttccc atcgctaccg
     4321 agtgctgaat ttcattcaac atttcaaaat cattattaga atccccgaat accataactt
     4381 catctaactc aaaaccatat cgatctgcaa cctttacaat acccgataat tttgaatttc
     4441 ccttactgat aatatcagtc gcatagggac tcgaacgcgt aaaagacaag ttcggaaaga
     4501 gcatctccaa tgattgtgtc tccctttccg tcgtcagcaa catcagctga tagattggtt
     4561 gaaagagaaa acctttcaaa ttcgtttcct gttgaggact aatccaacgt accaagcgat
     4621 tgaaaataaa atttatgatt ccagcccagg actccggaat catacgcgtc acacgatagg
     4681 caaaatttcc tgttccagca ctcataatct tactaccaaa tatgccttta gcagttccaa
     4741 aagataaatc cttatggtga gtttgcgcat actccataat ttgttcaatt tgctttggct
     4801 ctaaactctg actataaata acttcttctc ttgaaaaaat atattgacca ttataagcaa
     4861 tggccaaatc tagccctaaa ctagccatat actgcaaaac aaaacgaggg tcacgtcctg
     4921 tagctagacc aactaaaatc cccttctctt tcaaagcatt aatggcgaga atagttgaac
     4981 tactaaccat acgattgtcg gtcaataagg taccatcaat atcgaaaaat acagccttta
     5041 tcgtcaacca actcacctac tttctaagaa ttatgttaca attagtatat cacactttga
     5101 aagaaactga aaatttatga aaaaaattct agtactgcat acaggtggaa ccatttctat
     5161 gcaagccaat catcagggtg aagttgcatc gagtaaaatc aatccaatga cccaaattga
     5221 tagcccacta gaagaaattc aagtgacatc cttggatttt ctcaatgttc caagtccaca
     5281 cattcgctta gaacacatga tgactctata ccaaaaaatt aaagaagaac aaatgaactt
     5341 cgatggtttt gtcattacac atggcacaga tactctagaa gaaactgcct atttccttga
     5401 tacgatggct attccagaaa aaccaattgt attgaccgga gccatgcgtt cctcaaatga
     5461 actaggtagc gacggaattt ataactaccg aacagcccta cgtgtggctg ctgatgataa
     5521 atccgcagat aaaggtgttc tagtggtcat gaacgatgaa attcatgctg ctaaatatgt
     5581 tacaaaaacg cataccacca atgtctcaac ctttcaaaca ccaacccatg gaccactggg
     5641 attagtcacc aaacgagaga ttctctattt taaaactgct gaaccacgtg ttcgatttga
     5701 cttatcaaca gtcacaggaa cggtcccaat cataaaagct tatgcagata tggactccat
     5761 tctccttgat tctctcactg caagctcaat ttccggttta gtcattgaag cactcggcgc
     5821 cggaaatctt ccgcctaccg tccttccagc cattaagaaa ctccttgatc aaaagatacc
     5881 cattattctg gtatctcgct gttttaacgg aattgctgag cctgtctatg cctaccaagg
     5941 aggtggcatc caactggagc aagacggcat tctatttgtt aaagaattga atgctcaaaa
     6001 agcccgactc aaactcctta tcgctctcaa tgcaggcttg gaagagcaaa gcttggcaga
     6061 ttacatccaa ggttaatcgc tgacgaacta tacaatatgg gctctttgtc aactgtagtg
     6121 ggtagatgaa aagctaacac ctagagagga cgaacttcgt tctctctttc tttatctctt
     6181 tcttgtttta ggagataagc tgaaaagggc tcttcccagc ccttttca
//
LOCUS       DAZQCJ010000106         5990 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000106 DAZQCJ010000000
VERSION     DAZQCJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.107"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(64..1875)
                     /gene="glmS"
                     /locus_tag="ACHU66_001849"
     CDS             complement(64..1875)
                     /gene="glmS"
                     /locus_tag="ACHU66_001849"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR3390113.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVTGGEQAHL
                     VKAVGRIAELSAKVGDKTEGTTGIGHTRWATHGKPTENNAHPHTSQTAGHILVHNGVI
                     ENYAEIKEEYLAGHDLKGQTDTEIAVHLIGQFAEEGLSTLEAFKKALKIIQGSYAFAL
                     IDATDADTIYVAKNKSPLLIGLGDGYNMVCSDAMAMIRETSEYMEIHDKELVIVKKDS
                     VEVMDYDGNEIERGSYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLISAYTNEAGQ
                     VTVDADIIKAVQEADRIYILAAGTSYHAGFASKDFLEKLTDTPVELGISSEWGYNMPL
                     LSKKPLFVMISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREATYTMLLHAGP
                     EIAVASTKAYTAQIATLAILAKAVGDANGNAYAKEFDLVHELSIVAQSIEASLSEKDV
                     IAEKVEKLLSTTRNAFYIGRGSDYYVSMEASLKLKEISYIQCEGFAAGELKHGTISLI
                     EDGVPVLALISNHPHLASHTRGNIQEVVARGANVLTIVDEAVAKEDDDITVTTVHPFL
                     SAIAMVVPTQLIAYYATLQRGLDVDKPRNLAKSVTVE"
     gene            2182..3438
                     /locus_tag="ACHU66_001850"
     CDS             2182..3438
                     /locus_tag="ACHU66_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFR3390114.1"
                     /translation="MEQLYHTTELIGIKDKNIKILFILKHQTHLEIRAKLDYDSPGCP
                     HCQGKCIKYDFQKSSKIPILDCQGFPTLLLLKKRRFQCKSCQKVTVAETALVKKNCQI
                     SLPIWEKVTQLHTENMTNIAIARRLHISVSVVQRKLAQFQFEHDFTKLPKVLSWDEFS
                     RNKGKLAFIAQNFETRSIVTILDNNRQTTIKNYFYKYPRAVRETVEVVTVDMSGSYIP
                     ITKKLFPRAKIVLDRFHIIQHLSRAMMTTRIDIMKTFDTRSLPYRSMKNHWRILQKDS
                     RKLSLNRFFSRTFGQTVTPREVVQKTLNFSEELKFYYELYQILLFHFQEMNSKYFFEL
                     LEDNLDLVNPAFKTVFKTFLKYKTYITNAMELPYSNAKLEATNKLIKDIKRQAFGFRN
                     FTNFKTKIYIALNIKNERTNFVLSRC"
     gene            complement(3488..4030)
                     /gene="lepB"
                     /locus_tag="ACHU66_001851"
     CDS             complement(3488..4030)
                     /gene="lepB"
                     /locus_tag="ACHU66_001851"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR3390115.1"
                     /translation="MVKRDLIKQISLLVLLILGIIGLRFWLLEPVTITPEMANSYLKE
                     NDFIMTVRNVRPIHGDFILYNHEGKEYVSRVIALENETVTYMDDVLYRNDIIITENYL
                     KTPHSQESYTDDFTLETLTNGKYNVIPEGHYLVLNDVRTNQQDSRSFGLISSEAIVGR
                     LTFRISPLSEFGFIKTGLVQ"
     gene            complement(4073..4690)
                     /locus_tag="ACHU66_001852"
     CDS             complement(4073..4690)
                     /locus_tag="ACHU66_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HFR3390116.1"
                     /translation="MSDIMYPEVLTVGSGAVKVATVGDSLTYGYGLENREKDAYPCIL
                     AEKLGHHYQVSNYGLSGRSLQSTADFPYFKEKNAQLSLESEADIVIIMIGSNDSRGPY
                     WNRERFIREYREMAEQYMDLPSQPDVYLVIPPYVPTSRFGLNNQIIEDELQKIIPAIG
                     NELGLPVINLYTVTEGHLEYYSDGLHLTPLGNQVIAEEIYQHLRH"
     gene            4775..5884
                     /locus_tag="ACHU66_001853"
     CDS             4775..5884
                     /locus_tag="ACHU66_001853"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR3390117.1"
                     /translation="MKKQVIIIGGGIVGSTVAFYLSQEEQVNLTLIDSGIGTATRAAA
                     GIICPWMAQKKNKDWYKLTSDGAVFYRQLVADLEKSGATEIPFKQTGTIGLKSKPELL
                     DKIQKIAEERRVDTPTIGEITPLIGSEINQYLPLLKPDYFGIHLEGGGRIDGGRLIDI
                     LQEQFLKNGGTRIYGQAKLLDNHTVEVDNQVLTADHIILATGAWLPHILEPLGYQVDV
                     RPQKGQLFELDTEFDTDNWPVCMPYGEIDILPFENGKIIIGATHEDDMGYDLELDPEK
                     IQAMHDKIAEFMPDLANYPVARTRIGTRAYTSTYSPFYGNIEDMPNVWVASGLGSTGL
                     TNGPIIGWQIAKEILNQETNFDRTPYSPNRYILKK"
BASE COUNT         1863 a         1317 c         1061 g         1749 t
ORIGIN      
        1 aaaattttgt atattttttc aacagtgcaa aaagaaggct ggaaatccac gccttccttc
       61 ttcttattca acagtaacag acttagccaa gttacgtggt ttatcaacat ccaaaccgcg
      121 ttgcaatgtc gcatagtaag cgatcaactg tgtcggaacc accatagcaa tcgctgacaa
      181 gaatggatga acagttgtaa cagtgatgtc atcatcttct ttagctactg cttcatctac
      241 gattgttaag acatttgcac cacgcgcaac aacttcttga atattaccac gagtatggct
      301 agcaaggtgc ggatgatttg aaatcaaggc caagactggt acaccatctt caatcaagga
      361 aattgtgccg tgtttcaatt caccggccgc aaagccttca cattggatgt atgagatctc
      421 tttcaatttc aaacttgctt ccatagaaac gtagtagtca ctaccacgac cgatatagaa
      481 agcgttgcga gtagtcgata agagtttttc aactttttca gcaatcacat ctttttctga
      541 caagctggcc tcgatagatt gggcaacaat ggacaattca tgcaccaagt caaattcttt
      601 cgcgtaggca ttgccattgg catcaccgac agccttagcc aagattgcca aagtcgcaat
      661 ctgtgcagtg taggcttttg tagaagctac cgcaatttct ggtcctgcat ggagcaacat
      721 ggtgtaagtt gcctcacgag atagggtaga accaggaacg ttggttacag tcaaacttgg
      781 aatgcccatt tcattagctt taaccaatac ctgacggcta tcagcagttt cacctgactg
      841 gctaatcatg acaaagagtg gctttttgct caaaagcggc atattgtagc cccactcaga
      901 tgaaattccc aactctactg gtgtatctgt caatttttcc aagaagtctt ttgaggcaaa
      961 tcctgcgtga taggaagttc cagcagccaa gatatagatg cggtcagctt cttgtactgc
     1021 cttaataata tccgcgtcaa ctgttacctg accagcttca tttgtatagg cgctaatcaa
     1081 tttacgcata acagttggtt gctcatcaat ttccttgagc atgtagtatg gataagttcc
     1141 cttaccaata tctgacagat caagctcagc tgtatagcta ccacgttcaa tctcatttcc
     1201 atcatagtcc atgacttcaa cactatcttt tttcacgata accaattcct tatcgtgaat
     1261 ttccatgtat tcacttgtct cacgaatcat agccatagca tctgagcaga ccatgttata
     1321 gccatcacca agaccaatca aaagtggaga tttatttttg gcaacgtaga ttgtatctgc
     1381 atccgtagca tcaatcaaag caaaggcata tgaaccttga ataatcttca atgctttttt
     1441 aaaggcttct agagttgaca aaccttcttc tgcaaattga ccaatcaagt gtacagcaat
     1501 ttctgtatct gtctgccctt tcaagtcatg acctgcaaga tactcttcct tgatttctgc
     1561 gtagttttca atcacaccgt tgtgaacaag gatatgtcca gcagtttgag aagtgtgagg
     1621 gtgagcattg ttctcagttg gcttaccatg agtcgcccaa cgagtatgac cgatccctgt
     1681 tgtcccttca gtcttatcgc caacttttgc agacaattct gcaatgcgac caacagcttt
     1741 cacaagatga gcttgctcac cacccgtcac aaaaatacct gctgaatcat aaccacggta
     1801 ttctaatttt tcaagtcctt gaatcaaaat atcagttgcg tttgtatttc ctacaacacc
     1861 aacgattcca cacatacata tcacctacga tgtttgtcaa catcggcttt catttcttta
     1921 aaaattggtc tagtctaatt tgtatctaaa aagaccagtg aaatcagtat ataacaagtt
     1981 tttatccttg tcaacatttt aattggtcta gtctaatttc aaaatacagt tccagtcatc
     2041 tcaacacaat taaaaaaagg ctctataatt tctgtagtgg gtaaatccac tgtagagatt
     2101 atggaggctt tttgagtata tacaaaaagt cccatatgac ctataatgaa aagcaaccac
     2161 actcacatta gaaagactca tatggaacaa ctttatcata ccacagaact aattggaatc
     2221 aaagacaaaa acatcaaaat tctcttcatt ttgaaacatc agacccatct agaaatcagg
     2281 gcaaagctag attacgacag ccctggttgt cctcattgcc aaggaaagtg tatcaagtac
     2341 gactttcaaa agtcgtcaaa gattccgatt ttggattgcc agggctttcc aaccctcctt
     2401 ttgcttaaga aacggcggtt tcaatgcaaa tcttgccaaa aagtgactgt agctgagaca
     2461 gctctagtca agaaaaactg tcagatttcc ctacctattt gggagaaagt gacacaactc
     2521 catacagaaa acatgactaa tatagccatc gctaggagat tacatatctc cgtatcggtc
     2581 gtccagagga aactggctca gtttcaattt gagcatgatt ttacgaaatt accaaaagtc
     2641 ttgagttggg atgaatttag tcggaacaag ggaaaactcg cctttattgc tcagaatttt
     2701 gagacgagat cgattgtgac cattcttgac aacaaccgcc aaaccaccat caagaactat
     2761 ttctacaagt atcctagggc ggtcagagaa actgtcgagg tcgtaacagt ggacatgtcg
     2821 ggtagctata ttcccatcac caaaaaacta tttccaagag ctaaaattgt ccttgataga
     2881 tttcacatta tccaacacct gagccgtgct atgatgacga ctagaattga tattatgaag
     2941 actttcgata cgcgttccct accttatcga tccatgaaaa atcactggcg tattctccaa
     3001 aaagatagtc gaaaactgtc tctgaatcgc ttcttttctc gcacttttgg gcaaacagta
     3061 acaccgagag aggttgtcca gaagacattg aatttctctg aggaactgaa attctattac
     3121 gaactctatc agatccttct cttccatttc caagagatga actcgaaata tttcttcgag
     3181 cttctagaag acaatctgga tcttgtcaat cctgccttta aaactgtttt taaaactttt
     3241 ctaaagtata aaacgtacat cacgaacgcc atggagcttc catattccaa cgctaagctg
     3301 gaagcgacca ataaactcat caaagacatc aagaggcaag cgttcggctt taggaacttt
     3361 acgaacttta aaacaaagat atatattgct ttaaacatca aaaatgagag aacgaatttc
     3421 gtcctctcta ggtgttagct tttcgtctac ccactacagt tgacaaagag cctaaaaaaa
     3481 gctgattcta ctgaaccagc cctgtcttaa taaaaccaaa ttctgaaagt ggactaatcc
     3541 gaaaagtcag tctaccaaca atagcctcac tagaaatcaa accaaaacta cgactatcct
     3601 gttgattcgt tcgtacatca ttcaacacca aatagtgccc ctctggaatg acgttgtact
     3661 ttccatttgt caaggtttcc aatgtaaagt catctgtata actttcttgt gaatgaggcg
     3721 tttttaaata gttctctgta ataataatat cattacgata gagaacatcg tccatatagg
     3781 taaccgtctc gttttccaat gcaatcacac gactcacata ctcttttccc tcatggttat
     3841 acaaaataaa atctccatga atcgggcgga catttcgcac tgtcatgata aagtcatttt
     3901 ccttcaaata actatttgcc atctcagggg taatcgtgac tggctcaagt aaccaaaatc
     3961 gtaggccaat gattcccaaa atcaataaga ccaataaact gatttgtttt attaaatctc
     4021 tttttaccat aatctctcca tttcactagt attataccac aatttagagc cattagtgtc
     4081 tgagatgctg gtaaatctct tcagcaataa cttgatttcc taacggtgtc aaatgaagac
     4141 catcgctata gtattcaaga tgtccctccg tcacagtgta aaggttaata actggtaaac
     4201 caagttcgtt tccgatagct gggataattt tttgtaattc atcttcaata atttgattat
     4261 tcaatccaaa acgactggtt ggtacgtaag gcgggatgac taaataaaca tccggctggc
     4321 ttggtaaatc catatactgc tctgccattt ccctatactc tcgaataaac ctctccctat
     4381 tccaatatgg tcctcggcta tcgttactac caatcatgat gatgacaata tctgcttcac
     4441 tctcaagtga aagttgggca ttcttctctt taaagtaagg aaaatctgca gttgactgaa
     4501 gtgaacgccc actcaatcca taatttgaaa cttgatagtg atgcccaagt ttctcagcta
     4561 gaatacaagg ataggcatct ttttcccgat tctccaagcc atatccataa gtcaaactat
     4621 ctccgactgt tgcaactttt acggccccac ttccaacagt taggacctca ggatacatta
     4681 tatctgacac acgaactcct caatttttct tattttctat tgtatcatgt tgaaaaaaat
     4741 aaataaactt ccgaatttgt gatataatta acttatgaaa aaacaagtta ttattatagg
     4801 cggaggaatt gttggctcta ctgtagcctt ttatctctcc caagaagaac aagtcaatct
     4861 tactctcatt gattcaggta ttggaacagc cacacgagct gccgcaggta tcatctgccc
     4921 atggatggct cagaaaaaaa ataaagactg gtataagctg acttccgatg gtgctgtttt
     4981 ctaccgtcaa ttggtagccg atttggaaaa atcgggtgct actgaaattc cattcaaaca
     5041 gactggaacc atcggcctaa aaagtaagcc tgaactactt gataaaatac aaaaaattgc
     5101 cgaggaacgt cgcgtagata ctccgactat tggcgaaatt actccgctta taggctcgga
     5161 aattaaccag tacctgccac tacttaagcc agactatttt ggtatccatt tagaaggtgg
     5221 cggtcgtatt gatggtgggc gtttgattga tattcttcag gaacaatttt tgaaaaatgg
     5281 tggtacacgt atctacggac aagcaaaact gctcgacaac catactgtcg aagtagataa
     5341 tcaagtgctt actgctgacc atattatctt agccacaggg gcctggttgc cacatattct
     5401 tgaacctctt ggttatcaag tggatgttcg accacaaaaa ggacaattgt tcgaattaga
     5461 tacagaattc gataccgaca attggccagt ttgtatgcct tacggagaga ttgacatcct
     5521 accatttgaa aatgggaaaa tcattattgg tgcaactcat gaagatgaca tgggctacga
     5581 tttagaacta gaccctgaaa aaatccaagc catgcatgat aaaatagctg aattcatgcc
     5641 tgatttggca aactatcctg tagccagaac tcgcattgga acgcgcgcct atacttcaac
     5701 ttattcacca ttctacggaa atattgagga tatgcctaat gtctgggtag ctagtggtct
     5761 gggctccacc ggtctgacaa acggtccaat tatcggttgg caaattgcta aagaaattct
     5821 caatcaagaa acaaactttg accgtacacc atattctcca aatagatata ttctcaaaaa
     5881 ataagatacg atacgtttag attctactaa ctctttaaat gtagatacta agactagttg
     5941 ttatctaagc aataaatcat gaccacccgt caaacgggtg gtttgaacaa
//
LOCUS       DAZQCJ010000107         5973 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000107 DAZQCJ010000000
VERSION     DAZQCJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.108"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            106..1746
                     /locus_tag="ACHU66_001854"
     CDS             106..1746
                     /locus_tag="ACHU66_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFR3390118.1"
                     /translation="MSEKVEMQQSKEQATMARGLAWLTAGNILSRLLGVAYVIPWYIW
                     LGEYRAEANALFSMGYQIYANFLLISTAGLPTAIAKQVAKYNVLGKEEVSLYLVREFF
                     KLMLVFGAVFAGVMYLSAPWLADASGSKEKLLPVMYSLVPPLFIFPAMSILRGFFQGR
                     HDMKPYAISQLAEQLVRVIWILAATFMIMKLGSGDYLEAVVQSTFAAFVGMIASVGIL
                     VYTLWKQGYLGKLLHAKKQKISLDTGQLIKETVRDAIPIIILGLTIQLLQFIDQVTFI
                     RVMERITDYSNSELLELYSYMAANPSKITMMIIGISLSLGSVAIPLITEKFVKKDLKA
                     ASHLVADNLQLLFIFTIPAIVGTVLLAGPLYTIFYGPSEPIAITLFIWNLFLILPLGL
                     YSVISVVIQAIFENRRAIYYFLIGMLVKIVLQVPMIYVFKVYGSFISTIFGLGIMLYL
                     FYRRIDKVLHIDERLVIKDIATISWISIVMGLIVWGIEFVLNLILPANGYVSSFIHLA
                     VAGGAGMLVFVILTLKTRQLDRLIGSRAQSLRRKLRLG"
     gene            1913..2647
                     /gene="pyrH"
                     /locus_tag="ACHU66_001855"
     CDS             1913..2647
                     /gene="pyrH"
                     /locus_tag="ACHU66_001855"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR3390119.1"
                     /translation="MMKPKYERILIKLSGEALAGERGVGIDLKTVQEMAKEIQEVAES
                     GIQIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLKQLGVDTRVQ
                     TAIAMQSVAEPYIRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDANAVKFNELTHREVISRGLKIMDATASTLSMDNDIDLVVFNM
                     NEPGNIKRVVFGEPIGTTVSNSSEEK"
     gene            2781..3338
                     /gene="frr"
                     /locus_tag="ACHU66_001856"
     CDS             2781..3338
                     /gene="frr"
                     /locus_tag="ACHU66_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR3390120.1"
                     /translation="MSKEIIAKAQERMNQSHQSLAREFSHIRAGRANASLLDRISVEY
                     YGSPTPLNQLAGITVPEARVLLITPFDKSILKDIERALNASDLGLTPQSDGTVIRLVI
                     PALTEETRKNLAKDVKKVGENSKVAIRNIRRDAMDEAKKAEKAKEITEDELKTLEKDI
                     QKVTDDAIKTIDKMTADKEKELLEV"
     gene            3470..4324
                     /gene="cvfB"
                     /locus_tag="ACHU66_001857"
     CDS             3470..4324
                     /gene="cvfB"
                     /locus_tag="ACHU66_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HFR3390121.1"
                     /translation="MNDLLAQYIVGLVTDENDQFYFVQKEGRTFALSKDEGEHSLGQS
                     VKGFAYTDMKQKLRLTTKEVGASRTSFGWGTVTEVRKDLGVFVDTGLPDKQVVVSLDI
                     LPEIKELWPKKGDQLYVKLDVDKKDRIWALPAFQEDFQKMAGPAYDNMQNQNLRAIVY
                     RLKMNGTFVYLPDNNMLGFIHPSERFAEPRLGQVLEARVIGYRAVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSAPEDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DSFGTELV"
     gene            4334..5254
                     /locus_tag="ACHU66_001858"
     CDS             4334..5254
                     /locus_tag="ACHU66_001858"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR3390122.1"
                     /translation="MLVYIAGSGAMGCRFGYQLSKTNHEVILLDNWEEHIEAIRENGL
                     KVTGDVEETVHLPIMKPAEATREADFIILLTKADQLPKMLRDIKPIIGPTTKVLSLLN
                     GLGHATTMRRYVPDESIMVGVTMWLAGLKGPGHAHLEGPGSISVQHILGDEQSVADII
                     EMLNEAGLNVTYDNHVVSAIWQKACVNGVMNPTCTILDCTIGELFGTEAGLNLVQGIF
                     KEFIAVAKSEADGIDEEAIWKYVIDASKKASNHYPSMHQDLVQHGRKTEIDYLNGAVV
                     FKGKRAGIPTPYCQMITDMIHAKESMLGLR"
     gene            5264..5719
                     /locus_tag="ACHU66_001859"
     CDS             5264..5719
                     /locus_tag="ACHU66_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFR3390123.1"
                     /translation="MLIEAETIEDYLAALPENRKEAVERLHQVIVEQLPAGFEVGILG
                     AMINYYVPLAAYPDGYHCTPGEPLPFLALASQKAHIALYHMGIYMDQELNDWFVAAYQ
                     AQVPTKLDMGKSCVRMKNPKNIPYELIGDLVSKMSMERYIELYEKNHRK"
     gene            5725..5940
                     /locus_tag="ACHU66_001860"
     CDS             5725..5940
                     /locus_tag="ACHU66_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFR3390124.1"
                     /translation="MRRSFYSWLMTQRNPKSNEPVAILADYAFDESDFPKQSDNFDEV
                     SRFLEESASFAFSMSDFDAIWEDYLGH"
BASE COUNT         1703 a         1023 c         1438 g         1809 t
ORIGIN      
        1 cagatagaac tgaagttcat caaatcaagt caacaacgtc tgattttgat tttcgaagag
       61 tatgagtttt tcctgtctta tatttacata gaaagtaata gaaaaatgtc cgaaaaagtt
      121 gaaatgcagc agtccaaaga acaggcgaca atggccagag gcttggcttg gttgactgct
      181 ggaaatattc ttagtcgcct gcttggtgtt gcctatgtta ttccctggta tatttggtta
      241 ggggaatatc gagctgaagc caatgccctc tttagtatgg ggtatcaaat ctatgccaac
      301 tttttattga tttcaacagc tggtcttccg acagctattg ctaaacaagt agctaaatac
      361 aatgtcttgg gtaaagaaga ggtgtcgctt tacttggtca gagaattctt caagctcatg
      421 ttggtctttg gagctgtctt tgcaggggtc atgtatctaa gtgctccgtg gttggcagac
      481 gcatcggggt cgaaggaaaa gttacttccg gtcatgtata gtctagtccc gcctctcttt
      541 atcttcccag ccatgagtat cttgcgtggt tttttccaag gtcggcacga tatgaagccc
      601 tatgctatca gccagcttgc agagcagctg gtacgggtta tctggatact ggcagcaaca
      661 tttatgatta tgaagttggg tagcggtgac tatcttgaag cggttgtcca atctaccttt
      721 gcagcttttg tcgggatgat tgctagtgtt gggattttag tttatacttt atggaaacaa
      781 ggttaccttg gtaagttact tcatgctaag aaacaaaaga tttctttgga tacaggtcaa
      841 cttattaagg aaactgttcg agatgcgatt ccaattatca ttttaggtct aacgattcaa
      901 ttattgcagt ttattgacca agtcaccttt attcgtgtta tggaaagaat tacggattac
      961 agtaattcgg aacttttgga actttattca tatatggctg ccaatccaag taagattacg
     1021 atgatgatta tcgggatttc actgagtttg ggaagtgttg ctattccatt gattacagag
     1081 aaatttgtca agaaagattt gaaggcggcg tctcatttag tggcggataa tctacaatta
     1141 ctctttatat tcactatacc ggcaattgtt ggaacagtcc tgttagctgg gccactctat
     1201 acgatttttt acggaccgtc agagcctatc gccattactc tattcatttg gaacttattt
     1261 ttaattttac ctctgggact atattctgtc attagtgtgg tcattcaagc gatatttgaa
     1321 aatagaagag ccatttatta tttcttgatt gggatgctag tgaaaatcgt tttacaagta
     1381 ccaatgatct atgtctttaa ggtatatggt agctttatct caacgatatt tgggttggga
     1441 attatgctgt acctcttcta tagacgaatt gacaaggttc tgcatattga tgaaagacta
     1501 gtgataaaag atattgcgac cattagttgg atttctatcg taatgggact cattgtatgg
     1561 gggattgaat ttgtcttgaa tcttatcctt ccagcaaatg gctatgtatc tagctttatc
     1621 cacttggctg ttgcaggtgg agcaggtatg cttgtctttg tcatcttgac attgaagact
     1681 cgtcagctag atcgtttaat tggaagtcgg gctcagagtt tacggaggaa attgcgccta
     1741 ggctagtttg ttttaaaaat ctctattttc tactattgaa aagctgggaa aaagaggtgt
     1801 tttagaatgt gagaaggaaa gttcgttgag gattttcctt ttttcttgga gatttcagta
     1861 taaaaaaaac ggtttttgtg gtagaatagt agggataaaa aagaggagag agatgatgaa
     1921 acctaaatac gaacgtattc taatcaaact atctggtgaa gccttggctg gtgaacgtgg
     1981 tgtcggtatt gaccttaaaa ctgttcagga aatggcaaag gaaattcagg aagtcgcaga
     2041 atcaggtatt cagattgccc ttgttattgg tggtggtaac ctttggcgtg gagaacctgc
     2101 tgctgaagcg ggtatggatc gagttcaagc agactataca ggcatgttgg ggactgttat
     2161 gaatgctctt gtaatggctg attcccttaa acagcttggt gtggatacac gtgtgcagac
     2221 agcgattgcg atgcagtcag ttgcagagcc ttatattcgt ggtcgtgctc ttcgtcatct
     2281 tgaaaaaggt cgcattgtta tttttggagc tggtattggt tctccgtact tctcaacgga
     2341 tacgacagcg gctcttcgtg ctgcagaaat tgaagcggat gcgattttga tggctaaaaa
     2401 tggtgtggat ggcgtctaca atgctgatcc gaagaaagat gctaatgcag ttaaattcaa
     2461 tgaattgacc caccgtgaag tgattagtcg tggtttgaaa atcatggatg cgacagcatc
     2521 aactctctca atggacaatg atattgattt ggtcgtgttc aatatgaatg aaccaggaaa
     2581 tattaaacga gttgtctttg gtgagccaat cggtactact gtttcaaatt cttcagaaga
     2641 aaaataaatc atatcgtcat ttagtttttc ttttattact tcgttaattc gccttgccta
     2701 acttcggtta tacctgtggc tcgttgctta gtactaaaag taaactaaaa gactataaaa
     2761 aatacaaaga aggaagtctt atgtctaaag aaattattgc taaagcgcaa gagcgcatga
     2821 accaatctca tcagagtttg gcacgtgaat tcagccacat tcgtgctggc cgtgccaatg
     2881 ctagtttgtt ggaccgtatt tcagttgagt actacggttc tccaacgcca ttgaaccagt
     2941 tggctggtat tacagtgcca gaagcgcgtg ttcttttgat tactccgttt gataagtcaa
     3001 tcttaaagga tattgagcgt gctttgaacg cttcagatct tggtttgaca ccgcaatctg
     3061 acggtactgt tatccgtttg gttattcctg cccttacaga ggaaactcgt aagaacttgg
     3121 cgaaggatgt gaaaaaagtc ggtgaaaatt caaaagttgc catccgtaat atccgtcgtg
     3181 atgccatgga tgaagctaaa aaagctgaaa aagcaaaaga aatcacagaa gacgagttga
     3241 agacgcttga aaaagatatt caaaaagtaa cagatgatgc tatcaagaca attgataaaa
     3301 tgactgccga caaagaaaaa gaattgttgg aagtttaatc aacacgtttt tagactacaa
     3361 aaatagatca tcactgggct gtctaacttc cagtggtgaa ccaataagaa atctaatggg
     3421 gggagttaga caagacttcc cccacttttt aatcagaaag aagaaaatca tgaatgattt
     3481 attagcacag tatatcgttg gtttagtgac tgacgaaaac gatcagtttt actttgtcca
     3541 aaaagagggt agaacctttg ctctttctaa ggatgaagga gaacatagtc taggtcaatc
     3601 tgttaaggga tttgcctata ccgatatgaa acaaaaactc cgtttgacaa ctaaggaagt
     3661 tggggcgagt cgtaccagct ttggttgggg aactgtcaca gaggtacgta aggatttggg
     3721 tgtctttgtg gatacaggtc tgccagacaa gcaagtggtt gtttccttgg acattttgcc
     3781 tgaaatcaag gaactctggc cgaaaaaagg ggatcagttg tacgtcaaat tagacgtcga
     3841 taagaaggat cgcatttggg ctctgccagc ttttcaagaa gattttcaga aaatggctgg
     3901 acctgcttat gacaatatgc aaaaccaaaa tctgcgagcg attgtctatc gtttgaaaat
     3961 gaatggtact tttgtttatt tgccagacaa caacatgctt ggttttatcc acccaagtga
     4021 acgatttgcg gaaccacgtc tgggtcaggt tttggaggcg cgtgtcatcg gctaccgtgc
     4081 agttgatcgg accttgaact tgtctctcaa gccacgttca tttgagatgc tggaaaatga
     4141 tgcccagatg atattgactt atctggaaag caatggaggt ttcatgacct tgaacgataa
     4201 gtctgctccg gaagacatca aggccacctt tggtatttcc aaagggcaat tcaagaaagc
     4261 tcttggtggt ttgatgaagg ctggaaaaat taaacaggat tcttttggaa cagagttggt
     4321 ctagggggcg tctatgctag tatatattgc tggtagtggg gcaatgggtt gtcgttttgg
     4381 ttatcaattg tctaagacaa atcatgaagt gattttgctg gataattggg aagaacatat
     4441 cgaggcaatt cgagaaaacg gcttgaaagt gactggagat gtagaggaaa cagtacactt
     4501 acccatcatg aaaccggccg aagcaacgag agaagcagat tttatcattc tattgacaaa
     4561 ggctgatcag ttgccaaaga tgttgcggga tattaaaccg attatcggtc caactaccaa
     4621 ggttttgagt ctcttgaatg gtttggggca tgcgacgacc atgcgtcggt acgtgccaga
     4681 tgaaagtatt atggttgggg tgaccatgtg gttggcggga ctaaaggggc ctggacatgc
     4741 tcacttagaa ggtccgggtt ctatctccgt tcagcatatt cttggtgatg agcaatcagt
     4801 tgcggatatt attgaaatgc tgaatgaagc tgggctaaat gtgacctatg acaatcatgt
     4861 tgtcagtgcc atttggcaga aagcctgtgt caatggtgtg atgaatccaa cttgtaccat
     4921 tttagactgt acaattggtg aattatttgg tacggaggct ggactgaatc tagtacaggg
     4981 gattttcaaa gaatttattg ctgttgctaa gtctgaggca gatggaattg acgaagaagc
     5041 tatttggaaa tatgttattg atgcttcgaa gaaggcttcc aatcactatc catctatgca
     5101 ccaggatttg gtccaacatg gtcgcaagac agaaattgat tatctgaatg gagcagttgt
     5161 ttttaagggc aaacgtgcag gtatccctac gccatactgt cagatgataa cggacatgat
     5221 tcatgccaaa gagagtatgc tggggttgag ataggagaaa gtcatgctga ttgaagcgga
     5281 aacgattgaa gactatctgg ctgccttgcc tgaaaatcgg aaggaagcag ttgaacgact
     5341 ccatcaagtg attgtggaac agttaccagc tggttttgaa gtaggaatct tgggagctat
     5401 gattaactat tatgtgccgc ttgctgccta ccctgatggt tatcattgca caccgggaga
     5461 gcccctgcct ttccttgcct tggcatcaca aaaggcacac atcgccctct accacatggg
     5521 aatctatatg gaccaagaat tgaatgattg gttcgttgct gcttatcaag cacaggtgcc
     5581 taccaaactg gatatgggca agtcctgtgt tcgtatgaaa aatccaaaga atattcctta
     5641 tgaattgatt ggggatttag ttagtaagat gtctatggag cgctatatag agctctacga
     5701 aaaaaatcat agaaagtaga tgtagtgaga agaagttttt attcttggtt gatgacccag
     5761 cgcaacccga aaagtaatga accagtagct atcttggcag attatgcctt tgatgaatct
     5821 gattttccta agcaatcaga taatttcgat gaagtcagtc gttttttgga ggaatcggcc
     5881 agctttgcct tttccatgtc tgattttgat gcgatttggg aagattattt gggacattga
     5941 tggataaaat agtagaaaat agccttgttt ggg
//
LOCUS       DAZQCJ010000108         5945 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000108 DAZQCJ010000000
VERSION     DAZQCJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.109"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(830..1924)
                     /locus_tag="ACHU66_001861"
     CDS             complement(830..1924)
                     /locus_tag="ACHU66_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR3390125.1"
                     /translation="MFHFTTLFIGMDVHKESFLLCYYDMMANQFKHSTKVGPNVSYIV
                     NYVNELRRLYGQDAEVLCGYEAGCLGFTLYHQLQAHGIPCIVMAPTTVMKEGSKRVKT
                     DKKDAAQLAKALAFRSYRPVHIPTAEDEQVKEYIRMRTDHKVALKKIKQQLLAFCLRH
                     DFRYTEGRNNWTQKHVRWLHSLNPKGLYAEILTEYLLTYEKLVDQIARYDARIEQLGQ
                     SDSYQEEVSQLSCFIGVKTLTALSIVTEIGDFNRFATAQHSASYLGLTPSENSSGDKE
                     RRGAITKAGNSHVRRLLIEAAQSLAKGTIGYKSKELKRRQSGNRVEVIAYADKANERL
                     RRRYRTLVLGKNKKQNVAKVAIARELSGFI"
     gene            complement(2032..2706)
                     /locus_tag="ACHU66_001862"
     CDS             complement(2032..2706)
                     /locus_tag="ACHU66_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFR3390126.1"
                     /translation="MLKFIKHIASLFLFFVAYQIISGFLMVGPSLQAIPEFPAQLIEN
                     MIWICAIIGIVLGIAFTIVLWKFVYSRYTIDYSVSSSWFHKIQWPILLYIAFFIFQLL
                     VPISESQNQTLVIQFVSAYPLVSFLSVVIFAPILEELIFRGLLATYFFPKMVDMKAVG
                     IYLAVTGSLFSLVHMPTTILQFLIYFTMGLNLGWLYLIRRDIRYPIALHMLNNGISYL
                     MILFLV"
     gene            complement(2716..3033)
                     /locus_tag="ACHU66_001863"
     CDS             complement(2716..3033)
                     /locus_tag="ACHU66_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390127.1"
                     /translation="MKLFLWILVGFLFFNSLSDRKKVKQLQARVKKLQKTTKGENQMS
                     VLLKELVGSKAKIRFDEEISIACEYTILAVDEEWVKISRELAEGEIETKLVRVDNIVD
                     LSF"
     gene            complement(3043..3732)
                     /locus_tag="ACHU66_001864"
     CDS             complement(3043..3732)
                     /locus_tag="ACHU66_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFR3390128.1"
                     /translation="MNRLGNVKDWNVVKNWKFLLVGLAVVALNVLTQLAMFALPNFDG
                     NNLLIAAVLLLLAVVLGLILTGKLGLWKGEQKWGMVANVGFVVLAFIVMFGLKIIGGQ
                     LIMLEEGYGQTTANQEIINNSGLPTLVLFLFAVFFAPVLEELLFRGILMGRVFGKDSI
                     VGLLLSSFLFGLIHNPTNIGSWVVYGGMGLVLGLAYRISGKYTNALILHSLNNLLGFL
                     LMLLMQSLGLI"
     gene            complement(3758..3964)
                     /locus_tag="ACHU66_001865"
     CDS             complement(3758..3964)
                     /locus_tag="ACHU66_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390129.1"
                     /translation="MKENKKFISVLKIVYFVGLIGLGLYAVGYIFGEWLATGQIDLSN
                     LNILLPMVLGLPALLLFKKESDKN"
     gene            complement(3986..4744)
                     /locus_tag="ACHU66_001866"
     CDS             complement(3986..4744)
                     /locus_tag="ACHU66_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HFR3390130.1"
                     /translation="MKQGKQISTKQRWMRYSAYMLISAILGGFIGFFGVLIFKFSLPS
                     YLNLDNFLIFLRIITFLIFAGTVYFGVKANQNYKLYHSISDEDEEHVDELYKKMYRNL
                     EYATISFNVAVSLTLLNLVLGFGVTFFEDGAIMYGSIFDVVFYVILLISQIFIMKLTQ
                     KIRDYKLSAFATVKEMKDFAEAMDEGEKQANYEMSFQIVFTLNQIVLPGLYLFLFVLS
                     MLLQERQITAFLVVAFLHIYINVMQVRMIRRYFK"
     gene            complement(4741..4956)
                     /locus_tag="ACHU66_001867"
     CDS             complement(4741..4956)
                     /locus_tag="ACHU66_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR3390131.1"
                     /translation="MEYVLKNRLKELRARDSLNQTELAKLAEVSRQTISLIERGEYTP
                     SVVIAMRIAQIFNENVENVFQLVEVGE"
     gene            complement(4969..5535)
                     /locus_tag="ACHU66_001868"
     CDS             complement(4969..5535)
                     /locus_tag="ACHU66_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HFR3390132.1"
                     /translation="MEAKKKLFEVNIMKNKKFVWGLNILAVALILPFAFVFGMIGSWL
                     IANGSQYWELSVSEVWLLFPLYILLIVVHEAIHGIFFKVFCPENPVKYGFKWKSGMAY
                     ATSPGSLYNRMQMLVISLAPFVVISLGLTMLAGFGMMNTTLYFMLATMHAASCAGDFY
                     YVYLLLVKFAKENIAVEDTETGLIIYQA"
     gene            complement(5686..5880)
                     /locus_tag="ACHU66_001869"
     CDS             complement(5686..5880)
                     /locus_tag="ACHU66_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390133.1"
                     /translation="MKQPFIITGTTIARAVEIGSILLLAYILLFDLLGWSDMDTALMT
                     TLIASITCIEASLQMEKKRR"
BASE COUNT         1935 a         1325 c          942 g         1743 t
ORIGIN      
        1 tttatcattt attttaaata ctttcaaaac gaatcttgat ataatcttat aaaattcgag
       61 tatcttcatt ccccaaacaa taaaatatac ttaacacaaa tatgattttc ttcttcgatt
      121 ttatcaatca ttagtaaaca ttttacatgt cattattcat atattatcca aaattggcta
      181 tactacattc acgaaagtaa accatacaaa atgggagcgg gaagtgatac gactgactcc
      241 ctcactacca atttgccacc tcaaacaatc tccagaatat tcgaaaactc aaccactccg
      301 ccgagatgtt aataggaact caaacattaa catttttacc ctgtgtttac tactctaaat
      361 aaaaaatacc ctattgcaac gtgcaacaag gtaccatcta tctcaatcat atattctgta
      421 attcatttta tatagtcata tcagtttatc tttgattact tcgacgagcg aggaaacgtc
      481 gtttccttat ttccaaccta gaacagcttt taggctattc tagcaacctg tagctttggt
      541 tacctacagg tcaatggttc tgccgcgacg cgagtccgac tctctataag cgtggattac
      601 aattgtgcca cttagtcgtt tcgtagatga ctcaaacctt gaagtcctaa actccttcaa
      661 gatactttcc attcaaacat tatttcaatc cttatttctg tccaaattca cccagtgatt
      721 tgggatagaa atagggattc ctcatcgctc tgcgatgata aaacttaatt gtcaaaagtg
      781 tacatgaaaa taagcgctaa cttcaagcta ttcttcctgt catcatcccc taaataaaac
      841 cagataattc ccgtgcaata gctactttag caacattttg tttcttattt tttcctagaa
      901 caagtgtgcg ataacgtctt cttaagcgtt cattagcctt atccgcataa gcaatcacct
      961 ccactcggtt tccactttgt ctccttttca attctttgga tttataccca atcgttccct
     1021 tggccaatga ttgtgcagct tctatcagaa gtcgtctcac atggctattc ccagctttgg
     1081 tgatagcacc tcttctctcc ttgtcgccac tagagttttc gctaggagtc agcccaagat
     1141 aagaagcaga atgttgagct gtcgcaaagc ggttaaaatc accgatttct gtcacaatgg
     1201 aaagagcggt tagtgttttg acgccaataa agcaagaaag ctgagagacc tcctcttgat
     1261 aactgtcgct ttgacccagt tgctcaattc gcgcatcata ccgtgctatt tgatctacta
     1321 atttctcata ggtcaatagg tattctgtca aaatctctgc gtaaagtccc ttaggattta
     1381 gggaatggag ccagcggaca tgtttctgtg tccaattatt tcttccctcg gtatagcgaa
     1441 aatcatgtcg gagacagaag gcaagaagtt gttgtttgat tttcttcaga gccactttgt
     1501 ggtctgttct catgcggata tattctttga cttgttcatc ctcagcagta ggaatatgaa
     1561 caggccgata gctacgaaag gccagagctt ttgcgagctg agctgcatct ttcttatcag
     1621 tcttgacacg cttagatcct tccttcatca ccgttgtagg cgccatcacg atacagggaa
     1681 tcccgtgagc ttgtagctgg tgatataggg taaatccaag acatccggct tcgtagccac
     1741 ataacacttc tgcatcttga ccatataaac gacgaagctc attcacatag ttcacaatat
     1801 agctaacatt tggaccaact ttagtgctat gtttgaattg attcgccatc atatcataat
     1861 agcagagtaa aaaactttct ttgtgaacat ccattccgat gaaaagtgtg gtaaaatgaa
     1921 acatataaga cctccaattg agtgtggtaa ttcctgttag aagttgattg ttttttattc
     1981 tagtgtacag gtaaatccac gaattctcaa ctgggggtct ttacatattg tctataccaa
     2041 aaatagaatc atcaaatagg aaattccatt atttaacata tgtagcgcaa tcggatagcg
     2101 aatatctcgt ctaattaaat agagccaacc aagattgaga cccatagtga aataaattaa
     2161 aaactgcaga atggttgttg gcatatggac caaactaaac aaagaacccg ttacagcaag
     2221 atagattcca acagccttca tatctaccat tttagggaag aaataggtcg ccaacaaacc
     2281 tctaaaaatc aattcctcta aaatcggagc aaagattacg actgataaaa atgaaaccaa
     2341 ggggtaggca gacacaaatt ggatgaccaa ggtttgattt tgactttcag atataggtac
     2401 caaaagttgg aagatgaaaa atgcaatata gagcaaaata ggccattgaa tcttatggaa
     2461 ccatgatgaa gatacactat agtcaatggt ataccgagaa tacacaaatt tccataatac
     2521 aattgtaaat gcaattccga gaacaatgcc aataatcgca caaatccaaa tcatattctc
     2581 aatcaattga gctggaaatt ctggaatagc ttgtaatgac ggccctacca tcaaaaaccc
     2641 agaaataatt tgataagcaa caaaaaataa aaacaatgat gcaatgtgtt ttataaattt
     2701 taacatactc cctccctaaa aactcaaatc aacaatatta tcaactcgta ccaacttggt
     2761 ttcaatctct ccttccgcta attcacgaga aattttaacc cattcctcat caactgccaa
     2821 aatggtgtat tcacaagcaa tagaaatttc ctcatcaaag cggattttgg ccttacttcc
     2881 gaccaattct ttcaataaaa ctgacatctg attttctcct tttgtagttt tctgcaactt
     2941 ttttacccta gcttgcaact gcttcacttt tttcctatct gatagagaat tgaaaaatag
     3001 aaatcctact agaatccata aaaatagttt catcgtatcc tcctatatca agcccagact
     3061 ctgcatcagt agcatcaaca aaaatccaag aagattattc agactgtgta gaataagtgc
     3121 attggtatat ttgccagaaa tacgataggc gagtcccagg accaggccca tacctccgta
     3181 gacaacccaa gatccgatat tggttggatt gtgaataagt ccaaacaaaa aacttgatag
     3241 caataatccc acaatcgaat ccttaccaaa tactctcccc ataagaatcc ctctgaaaag
     3301 cagttcctcc aaaactggag caaagaaaac tgcaaagaga aacaaaacta aggttggtaa
     3361 gcctgaattg ttgatgattt cctgattggc agtcgtttga ccatatccct cttccaacat
     3421 aatcaattgc ccaccaatta ttttcagacc aaacataacg ataaaagcga ggactacaaa
     3481 cccaacattc gcaaccatcc cccatttctg ctcacctttc catagaccta atttaccagt
     3541 caagataaga ccaagcacca cggctaacaa taaaagaact gcagcaatca gtagattatt
     3601 accgtcaaaa tttggtaggg caaacatggc caactgggtc aatacattca gtgcaacgac
     3661 tgccagtccc actaagagaa acttccaatt tttaaccaca ttccaatctt tgacatttcc
     3721 aagtctattc atagcaatct ccttcacata cactaaatca attcttatca ctttcttttt
     3781 tgaacaatag cagagctggc aagcccaaaa ccatcggcaa gaggatattc agattagaca
     3841 aatcaatctg cccagtagcc agccattctc cgaaaatata gccaactgcg tacagaccga
     3901 gaccgattaa ccccacgaag taaacaattt ttagaaccga tataaacttt ttattctctt
     3961 tcataatcac taccaaaacc ttcccttatt taaaataccg acggatcatg cgaacctgca
     4021 tcacattgat ataaatgtgc aaaaacgcca caactagaaa tgccgtaatt tgtcgttcct
     4081 gcaatagcat gctaagtaca aataggaata gatacaaacc tggtaacacg atttgattca
     4141 aagtgaagac gatttggaaa ctcatttcgt agtttgcttg tttctcacct tcgtccatcg
     4201 cctcagcaaa gtccttcatt tcttttaccg tagcaaaagc ggataactta tagtcacgaa
     4261 ttttctgagt taatttcatg ataaaaatct gagaaatgag aagaatgaca tagaaaacta
     4321 catcaaaaat tgacccgtac atgatcgccc cgtcttcgaa gaaggtaaca ccaaatccta
     4381 gaaccaaatt taacaatgtc aatgatacag caacattgaa tgaaatggtc gcgtactcta
     4441 ggttacgata cattttctta tatagttcat caacatgttc ctcatcctca tctgaaatgg
     4501 aatgataaag tttataattt tgattagctt taacaccaaa atatacggtt ccagcgaaaa
     4561 ttaaaaatgt aataatcctc aaaaaaatta gaaaattatc taaattgaga taagaaggta
     4621 ggctgaattt aaagattaat actccaaaga aaccgataaa gccacccaaa attgctgaaa
     4681 tcagcatata cgctgaatat ctcatccatc tttgtttcgt tgaaatttgt ttcccctgtt
     4741 tcattctcct acctccacta attgaaaaac attctccaca ttttcgttaa aaatttgagc
     4801 aattcgcatg gcaatgacca ccgagggcgt gtactccccc cgctctatca gactaatggt
     4861 ctgccgtgat acttctgcta actttgccaa ctcagtctgg ttgaggctat ctcgtgcccg
     4921 caactccttg agccgatttt ttaagacata ttccatgatg tttcctttct aggcttggta
     4981 gataatcaag cctgtttctg tgtcttcgac agctatattt tccttggcaa acttgaccaa
     5041 taagagatag acataataga aatcgcctgc acaagaggcc gcatgcatgg ttgccagcat
     5101 gaagtaaaga gtggtattca tcatcccaaa tcctgctaac atggtcaagc ccaaactaat
     5161 cacaacaaag ggagccaagc tgattactag catttgcatc cgattgtaga gcgaacctgg
     5221 actggtcgca taggccatac ccgatttcca tttaaacccg tatttgacag gattttcagg
     5281 acaaaaaact ttgaagaaga tgccgtggat agcctcatgc acaactatta aaagaatata
     5341 gagtgggaaa agcaaccata cctccgacac gctcagttcc cagtattgcg aaccgttagc
     5401 tatcaaccaa ctgcctatca ttccaaatac gaaggcaaat ggaagaatga gtgccacagc
     5461 aagaatattc aatccccaca caaacttctt atttttcatg atattgactt caaataattt
     5521 ctttttcgct tccatgtttc ttctccttca tttgcctaca agctagacta aactatatag
     5581 tcctagagca actccaatca gtaagccgat aaataattgc ccctgcaatc ataatcgcta
     5641 ccatatctgc tgaaatatta cgtttcatgg tctatctcct attacttatc tccttttttt
     5701 ctccatttgt aaacttgctt caatacaagt aattgaagca attagagtag tcattagagc
     5761 tgtatccata tcgctccatc ccagcaaatc aaaaaggaga atgtaagcca ataatagaat
     5821 ggaccctatt tcgactgcac gcgcaatggt tgttcctgta attataaaag gttgtttcat
     5881 ttggtgactc cttatctgtt catcgtatca gatgaacctt ttcgattttc atctcaatat
     5941 cattg
//
LOCUS       DAZQCJ010000109         5937 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000109 DAZQCJ010000000
VERSION     DAZQCJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.110"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(60..1565)
                     /gene="pyk"
                     /locus_tag="ACHU66_001870"
     CDS             complement(60..1565)
                     /gene="pyk"
                     /locus_tag="ACHU66_001870"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR3390134.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGDDGYWGEKLDVESSAQKIAA
                     LITEGANVFRFNFSHGDHQEQGDRMATVRRAEEIAGKKVGFLLDTKGPEIRTELFEDD
                     AKEYAYTTGDKLRVATKQGIKSTKEVIALNVAGGLDVFDDVEVGKQILVDDGKLGLTV
                     VEKDAVNREFVVLVENDGVIAKQKGVNIPYTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVEVVRNICKETGNDHVQLFAKIENQQGIDNIDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIITKVNAAGKAVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIAKNAQTLLNEYGRLNSDQFNRASKTEVIASAVK
                     DACHSMDIKLVVTVTETGYSARSISKYRPDADILAVTFTEKVQKSLMLNWGVIPIVTN
                     RPDSTDDMFDLAERIAKEQGLVEAGDDIIIVAGVPVGVAGSTNTMRVRTVK"
     gene            complement(1632..2642)
                     /gene="pfkA"
                     /locus_tag="ACHU66_001871"
     CDS             complement(1632..2642)
                     /gene="pfkA"
                     /locus_tag="ACHU66_001871"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR3390135.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVYGINEGYAGM
                     VAGDIHELSARSVGDIISRGGTFLGSARYPEFAKLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGVPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRHAGDIALWAGIASGADVIVVPEEDFNINDIVDRIKAGYDNGKKHSII
                     VLAEGVMPAAQFAEELKAAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGRGGLAVGIRNEQMVENPILGTAEEGALFSLTTDGKIVVNNPHKADLELADLNRNL
                     SI"
     gene            complement(2727..5837)
                     /locus_tag="ACHU66_001872"
     CDS             complement(2727..5837)
                     /locus_tag="ACHU66_001872"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR3390136.1"
                     /translation="MLAQLDTKTVYTFMDSMVTIENYVGRAKELGYHALGIMDKNSLY
                     AAYSFMESCNKMGLQPIIGCELDWKLQEGEQDVTTQLIALTTRGYRNLLKISTAKMIG
                     QDDFETIRQYLVDIAVVVPYFSGIEHLELGVNYYIGVTVDTPVQDFKQAILPIHTVRF
                     FEEEQLESLQVLHAIRDNIPLNQVVDIDSRQKLLSADSLTNLFSNRFPQAVDNLRDLV
                     AGISYQLNTDLKLPRFNRDRPAVEELYERSYQGLEEKGLTNSLYKERLEKELSIIHQM
                     GFDDYFLIVWDLLRFGRSQGYYMGMGRGSAVGSLVAYALNITGIDPVKHNLLFERFLN
                     AERYSMPDIDIDIPDIHRADFIRYVRDRYGSTHAAQIVTYSTFGAKQAIRDVFKRFGT
                     PEYELSNITKKISFRDSLTSAYQRNAAFRQIINSKIEYQKAFAIAQQIEGQPRQTSIH
                     AAGIVMSDEDLTDTIPLKIGDDMLVTQYDAHAVEANGLLKMDFLGLRNLTFVQKMAEG
                     VRERYQQEIDIAKIDLEDKKTLELFAAGRTKGIFQFEQPGAISLLRRVKPGRFEDIVA
                     TTSLNRPGASDYSDNFVKRKHGQETVDLLDPTIAPILQPTYGIMLYQEQVMQIAQVYA
                     GFTLGKADLLRRAMSKKNAQEMQVMEAEFLQGALKNGHAEENAREIFGMVAKFAGYGF
                     NRSHAYAYSALAFQMAYFKSHYTDVFFDVMLNHSSGAYIEDAMQFDFEVARLTINNIP
                     YHDKFDKSKVYLGLKTLKGLPKDLALWILDHRPYKSVEDFILRLPDNFKKKDLLIPLI
                     QIGLFDDFVANRKKIIENLDNLFVFAEAIGTFFAEESYSWTDVEDYSDSEKFMLEQEL
                     LGVGISPHPLVNICRESKRSFTDLADLAVGNRATVLVQIQAIRLIRTKKTGEQMAFLR
                     VTDTKRTLDVTLFPESYRQYASILEEGGMGYITGRVQERDGQLQLVLEKLEPVSLEKF
                     WILLENREHDYAVARILDKYRGSIPVVLHYQDSNQTIQAEHHMVMKTPQLEEELSEFV
                     MKTIFR"
BASE COUNT         1749 a         1374 c         1004 g         1810 t
ORIGIN      
        1 acagcacgta tttaacttat tctctaatta ctacaaaaag gtccaagcgg acctttttat
       61 tattttactg tacgaacacg catagtgttt gttgaacctg caacacctac aggcacacca
      121 gcaacgataa taatgtcatc tcctgcttca acaagacctt gctctttggc aatacgttca
      181 gccaaatcaa acatatcgtc agttgaatct ggacggtttg ttacaattgg aataacaccc
      241 cagttaagca tcaatgattt ttgtactttc tcagtaaatg ttactgccaa gatgtccgca
      301 tctggacggt atttagagat cgaacgagct gagtaacctg tttctgtaac tgttacaact
      361 agtttgatgt ccattgagtg acatgcgtct tttactgcag aagcaataac ttctgtttta
      421 gaggcacgat taaactgatc agagttcaaa cgaccatatt cattaagaag agtctgtgcg
      481 tttttagcga tagttgccat tgtacgaact gattctactg gatatttacc gtttgcagac
      541 tcacccgaaa gcattgttgc gtcagtaccg tcgataactg cgttgaatac gtcagatact
      601 tcagaacgag ttgcacgtgg tttttcagtc attgtttcca acatgtttgt tgctgtgata
      661 actgctttac cagctgcatt cactttagtg ataatcattt tttggtaaac tggaaccatt
      721 tcgaatggta cttcgatacc catgtcacca cgagcaatca tgataccatc tgctgcttcg
      781 atgatttcgt cgatgttgtc aataccttgt tggttttcga ttttcgcaaa caattgaacg
      841 tggtcgttac cagtttcttt acagatgtta cgaacaactt ctacgtcttt tgcagtacgt
      901 acaaatgaga tagcaataaa gttcaaacct tgctcaagac cgaagcggat atctgcatta
      961 tcacgctctg caagagctgg gaaaggaatt tttgtgtaag ggatgtttac acctttttgt
     1021 ttagcaatta caccgtcgtt ttcaaccaaa acaacaaatt cacggtttac tgcatctttt
     1081 tcaacaactg tcaaaccaag tttaccatcg tcaacaagga tttgcttacc aacttcaaca
     1141 tcgtcaaaga catccaaacc acctgctacg ttcaaggcaa tcacttcttt tgtagatttg
     1201 ataccttgtt ttgtagcaac acgcaatttg tcaccagttg tatatgcata ttcttttgca
     1261 tcatcttcaa acaattctgt acggatttct ggaccttttg tatccaacaa gaaacctact
     1321 tttttaccag cgatttcttc tgcacgacga actgttgcca tacgatcacc ttgctcttgg
     1381 tggtcaccgt gtgagaagtt gaaacggaaa acattggcac cttctgtaat caaagcagca
     1441 attttctgtg ctgatgactc aacatctaat ttttcacccc agtaaccatc gtcaccgaat
     1501 tttttaccac cgcggatttc taccgctgga ccaagggttg caacaatttt aacacgtttg
     1561 ttcatcttaa caaaactcct tttatttcaa tggtcatacg acccttaatt aacactttta
     1621 aacaacgata cctagattga caagttacgg ttcaaatcag ccaactccaa atctgcttta
     1681 tgcggattat taactacaat cttgccatca gtagtcaagc taaacaaggc accttcttct
     1741 gcagttccaa gaattggatt ttcaaccatt tgctcgttac ggatacctac tgcaagacct
     1801 ccacggcctt ctttaagcaa tttaacggca tgagcaccca tacgagaagc gagtacacga
     1861 tcacgcgctg ttggagaacc accacgttgg atatgaccaa gttctgtcac acgaagatca
     1921 cttgtatcgc ctgctgcttt caattcttca gcgaactgtg cagctggcat aacaccttca
     1981 gccaaaacaa tgatgctatg tttcttacca ttgtcgtagc ccgctttaat acggtcaaca
     2041 atatcgttga tattgaagtc ttcttctgga acaacgataa catctgcacc agaagcaata
     2101 ccagcccaaa gagcgatgtc tcctgcatga cgtcccatta cttcaatgac gaaagtacga
     2161 cggtgacttg atgaagtatc gcgaatctta tcaatcgcat ccatggcagt tgttacagct
     2221 gtgtcaaaac cgattgtaaa atctgtcccc acgatgtcgt tgtcaatggt accaggtaca
     2281 ccaatagctg ggaaaccatg ctccgtcaag cgcattgctc cgtggtaaga accgtcacca
     2341 ccgataacca ctacaccttc gataccatgt tttttcaatt gctcaatccc tttaagttgc
     2401 ccttctaatt tggcaaattc tggataacga gctgaaccaa ggaatgttcc tccacgtgaa
     2461 ataatatcac ctacagaacg tgcagacaat tcatggatat cacccgcaac catacctgcg
     2521 taaccttcgt tgattccata aacttccatt ccttctgaaa ttgcttggcg aacaactgca
     2581 cgaatagcag cgttcatacc aggggcatca ccaccactgg tcaaaacagc aatacgtttc
     2641 attcgttaat gctcctttaa cttttcattt taactgccct attataacat acttagccta
     2701 aacttgcttg actttttgct atttttttat cgaaaaatcg ttttcataac aaattcactt
     2761 aactcttctt ctaattgagg cgttttcata accatgtggt gctcagcctg aattgtctgg
     2821 ttactatctt gataatgaag tactactgga atgcttcctc tatatttatc caaaatacga
     2881 gcaacagcat aatcgtgttc tcgattttcc aaaagaatcc aaaatttctc caaactgaca
     2941 ggctctaatt tttctagaac taattgcaac tgtccatcgc gctcttgcac tctacctgtg
     3001 atgtacccca ttcccccttc ttccaaaata gaagcgtatt ggcgataaga ttctgggaaa
     3061 agagtcacgt caagcgtcct cttggtatct gtcactcgaa gaaatgccat ctgctcccca
     3121 gtctttttgg tacgaatcaa tcgaatagct tgaatctgca caagaacggt cgctcgattg
     3181 ccaaccgcta aatcagccaa atctgtaaaa ctacgcttag actctcgaca aatattgact
     3241 aaaggatgcg gactaatacc aacacctagc aattcttgct ctagcataaa tttttcactg
     3301 tcgctgtaat cttctacatc tgtccaacta taactctcct ctgcaaaaaa tgtaccaatc
     3361 gcctctgcaa agacaaagag attgtctaga ttttcaataa tctttttacg attggcgaca
     3421 aaatcatcaa ataaaccaat ctgaataaga gggattagta aatctttttt cttgaaatta
     3481 tctggcaagc gcaaaataaa atcttctaca gacttatagg gacgatgatc caatatccat
     3541 aaagctaaat ctttaggtaa accttttagt gtttttaatc ctaggtagac cttactttta
     3601 tcaaacttat cgtgataagg aatattgtta atcgtgagtc gagctacctc aaagtcaaac
     3661 tgcatcgcat cttcaatata agcaccgcta gaatgattga gcatgacatc aaaaaagaca
     3721 tccgtatagt gactcttgaa ataggccatt tgaaaggcta aggctgaata agcataggcg
     3781 tggctgcggt taaaaccata accagcgaac ttagccacca tgccaaatat ttcccttgca
     3841 ttctcttcag catgtccgtt tttcagtgct ccctgtaaaa attccgcttc cattacctgc
     3901 atttcctggg cattcttttt agacatcgcc ctgcggagta aatccgcctt tccaagcgta
     3961 aatcctgcat agacctgtgc aatctgcatg acctgttcct gataaagcat gataccataa
     4021 gtcggttgta aaataggagc aatggttggg tccaacaaat caactgtctc ttgtccatgt
     4081 ttgcgtttta caaagttatc cgaataatca ctagcacccg gtctattcaa actcgttgtt
     4141 gcaactatat cctcaaatcg accaggtttg acccttcgaa gcaggctaat cgcaccaggc
     4201 tgttcaaatt gaaaaattcc cttagtccga ccagcagcaa aaagttccaa ggttttctta
     4261 tcttctaaat ctatcttggc aatatcaatt tcctgctgat aacgttcacg tactccttct
     4321 gccatttttt gcacaaaggt cagatttcgt aagccaagga aatccatctt caacagacca
     4381 ttcgcctcaa cagcatgtgc atcatattgg gttaccagca tgtcatcccc tattttaaga
     4441 gggatggtat ctgtcaaatc ctcatcactc atcactatcc ctgctgcatg gatagaagtc
     4501 tgtcggggtt gcccttcaat ttgttgagca atcgcaaaag ctttttggta ctctatctta
     4561 ctattgataa tctgacgaaa agcggcattt cgctgatagg cagaagtaag actatcccta
     4621 aatgaaattt tcttggtaat attacttaac tcatattcag gtgttccgaa acgcttaaat
     4681 acatcacgaa tggcctgctt agctccaaat gtcgagtagg ttacaatttg cgcagcatgg
     4741 gttgaaccat aacgatctcg tacataacga ataaaatctg ctcgatgaat atctggaata
     4801 tcaatatcaa tatcgggcat tgaataacgt tcagcattga gaaagcgttc aaagagaaga
     4861 ttatgcttaa caggatcaat acctgtgata tttaaggcgt aggcaactag gcttccaaca
     4921 gctgaaccgc gccccattcc catgtagtac ccttggctac gcccaaaacg aagtaggtcc
     4981 catacaatca agaaataatc gtcaaatccc atttgatgaa taatggataa ttccttttcc
     5041 aatcgttcct tatacagtga attagtcaag cccttctctt ctaatccttg ataggagcgc
     5101 tcgtataatt cttccactgc aggtctgtca cgattaaaac gcggcaattt taaatctgta
     5161 tttaattgat aagaaatccc tgcaaccaag tcacgtaaat tatcaactgc ttgcggaaac
     5221 cgattcgaaa atagattggt taaactatca gccgataaaa gcttttgtcg actatctata
     5281 tctacaactt gattaagtgg aatattatct cgaatagcat gtagaacctg tagactttcc
     5341 aattgttcct cttcaaaaaa acgaaccgta tgaattggga gtattgcttg cttaaagtct
     5401 tgaactggtg tatcaaccgt cactccgata taatagttaa cacctaactc aaggtgctca
     5461 atgccagaaa aataaggaac aaccactgct atgtcgacta aatattgacg aatagtttca
     5521 aaatcatctt gccctatcat cttagcagtc gaaattttca atagattgcg ataacctctg
     5581 gttgtcagag caatcaattg agtagttacg tcctgctccc cttcctgtaa tttccagtcc
     5641 agttcacaac caataattgg ttgaagcccc attttgttac aggattccat aaaactatag
     5701 gctgcataaa gactgttttt atccattatc cccaatgcgt gataaccgag ctctttagcc
     5761 ctgcctacat aattttctat cgtcaccatg ctatccataa aggtataaac tgtctttgta
     5821 tctaattgtg ctagcatggc attctccttt tgttgcttct tcttaaatta tatcaaataa
     5881 tcatcaaaaa atattttaaa aaaattcaaa acatttccct tgctaattta tatgatt
//
LOCUS       DAZQCJ010000110         5743 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000110 DAZQCJ010000000
VERSION     DAZQCJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.111"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(58..606)
                     /locus_tag="ACHU66_001873"
     CDS             complement(58..606)
                     /locus_tag="ACHU66_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390137.1"
                     /translation="MSQLTLSSKNSVKQLSISAILTAFAILIPLMMPIKIIIGPASYT
                     LASHIPLFIAMFISPATAIFVALGSSLGFFLAGFPIVIVFRALTHLFFLTLGAVLVKR
                     FPTLMDSKRFLLLGIGLNLLHGLGEYIVVMVLTSGQQTSATYWITMLGLVGVGSAIHG
                     LLDFSLAYYFWKILKERKIYQP"
     gene            complement(686..1204)
                     /locus_tag="ACHU66_001874"
     CDS             complement(686..1204)
                     /locus_tag="ACHU66_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFR3390138.1"
                     /translation="MKATERRKKILSLLRESPYPLAANYLAKEFGVSRQVIVGDVALL
                     RAEGEAVLATPRGYLLQSSLPSTEGAYIRKIACQHGPEEVEKELTILLEGGAQILDVE
                     IEHPIYGLLSGKLNIASLEDRDHFLDALGQYQGVLLSNLTEGVHTHTVSFPSVQAYNE
                     LSAALKHAGILL"
     gene            complement(1310..2185)
                     /locus_tag="ACHU66_001875"
     CDS             complement(1310..2185)
                     /locus_tag="ACHU66_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR3390139.1"
                     /translation="MQTIIPHLWYDTEAKEAVAFYVELFGGKIDWTYTITDTPSGDSD
                     LIQFQLGDMTLAAISAGPYFKLNESMSLMVNVASKDEVTRLYQVLSEGGRVLMPLGEC
                     PFSPYYVWLEDRFGLSWQLSYAPDLDKPYQFDICLLFSQEQVGLAQPMLDYYKDKLHQ
                     ARLGRLSYYGEGEASVAAAKLNYAELFIGDQKIIAMDHGYGGVASFNEAFSFMVYVDS
                     QEEAESWYEKVSAVPEAEICGWAKDQFGISWQIVPRILMEAYDTANPEKVKAVNAAVM
                     TMKRLDIAAIQALLN"
     gene            2306..2806
                     /locus_tag="ACHU66_001876"
     CDS             2306..2806
                     /locus_tag="ACHU66_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFR3390140.1"
                     /translation="MEIWNTYTADGHLTDHTLTRGESIPDGLYHLVVECIIRHHDGST
                     LFMKRDSTKPSYPDYYEATAGGSALFGEIAEQAILREVREETGIELTADQLRHHTHFV
                     AHDDQCIFHCYWAETDWDKSTIQLQSNETSNYIWVPQENLKNFLETELVIPRQKDYVE
                     RLFLKS"
     gene            complement(2861..4315)
                     /gene="gltX"
                     /locus_tag="ACHU66_001877"
     CDS             complement(2861..4315)
                     /gene="gltX"
                     /locus_tag="ACHU66_001877"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR3390141.1"
                     /translation="MTKPIRVRYAPSPTGLLHIGNARTALFNYLFARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGIDWDESPETHEQYRQSERLDIYQKYVDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEYLGMSEDEKAAYIAEREAAGIVPTVRLAV
                     NETGIYKWNDMVKGEIEFEGGNIGGDWVIQKRDGYPTYNFAVVVDDYLMKISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRRKGYM
                     PEAVFNFIGLLGWNPGGEEEIFSREQFIQLFDENRLSKSPAAFDQKKMDWMSNEYIKN
                     ADLETIFNLAKPFLEEAGRLTDKAEKLVELYKPQLSSADEIVGLTDLFFSDFPELTAE
                     EKEVMAGETVPTVLNALKEKLEAMTDEDFQPDNIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPNTIYLLGREKSIQHIDNMLKSL"
     gene            complement(4387..5667)
                     /locus_tag="ACHU66_001878"
     CDS             complement(4387..5667)
                     /locus_tag="ACHU66_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR3390142.1"
                     /translation="MKKILKNATYLWVLTADMLSNFGDVVYYLALMNYVLLVPNSRLA
                     LAIVTFSEIFPSFMGLFTGYLADKTVNKISTIKLTLLFRVLLYLILGFCMGFEPALWI
                     VVVAATFNVFADFAGFYENGLYTPLGLRVAPKEEREQYSAFRQTVTSLLNIAFQALSA
                     LLVGLLSYQNLAFLNAGTFLAALLIMQLLTPVFRKLLTEQPLKIAEQSSQTEQKTKRP
                     GILASFKQAIVELRKIPEFRLVLITTPLINACGAILYPILVLLISEDPGLVFLNVETT
                     LALTILIFFIGHILGSTLVFTLFKNTSMVTLEVAATFSLLGVFVGMLLHQLPIIFFFL
                     TTMGISSGASGPKFNAKFVNSMPEEQLATIGGAVSTYFMLGQAFTRLLVSGLVLILTV
                     NQISGLFLAATGLLVLYILYWLARNQKTPQNQSV"
BASE COUNT         1667 a         1367 c         1241 g         1468 t
ORIGIN      
        1 caaaaaaagc tagagaatat tctccagctt ctaaaatata ttcctcatca taactgatta
       61 tggttgataa attttccgtt cttttaaaat tttccagaaa taataagcta aactaaaatc
      121 aagcaatcca tgaatagccg agccaacacc caccaagcca agcatcgtta tccagtaggt
      181 agcagaagtt tgttgccccg aagtaagcac catcaccaca atatattctc ctagaccatg
      241 caggagatta agcccaattc ctaatagtaa aaagcgctta gaatccatga gagtcggaaa
      301 acgcttgacc aagaccgcac ctaaggtaag gaagaacaga tgcgtaagcg cccgaaatac
      361 aataacgatt ggaaaaccgg cgagaaaaaa gcctaaacta gaacctaaag caacaaaaat
      421 agcagtagct ggtgaaataa acatggctat aaacaaagga atgtgactag caagtgtata
      481 ggatgctggg ccaataatga ttttaatcgg catcattaaa ggtattaaaa tggcaaatgc
      541 ggttaaaatt gcactaattg atagctgctt aactgaattt ttagatgata gtgttaattg
      601 agacattagg cacctctctt tctattggtt tgattataaa acaaaaacat tacatgtgtc
      661 aagacacctg taatgtttat tgattttata gtaaaatacc tgcatgtttt aaagctgctg
      721 aaagttcatt ataggcttgc acagatggga aagagactgt atgtgtatga actccttctg
      781 ttaaattact cagtaacaca ccttggtatt gacccaaggc atccaaaaaa tgatccctat
      841 cttctaaact tgcaatattc agcttgcctg ataataagcc gtaaatggga tgctcaattt
      901 caacatctag tatttgagct ccaccctcta gcaagattgt caattctttt tctacctctt
      961 ctggaccatg ttgacaagct atctttcgaa tgtaagctcc ttcagtagat ggcaaagaag
     1021 attggagtaa gtaccctctt ggagttgcca agacagcttc tccctccgca cgaagcaagg
     1081 ctacatctcc aacaattact tgccgactca ctccaaattc tttcgccaga taattagctg
     1141 caaggggata ggggctttct cgtaaaaggg ataggatttt cttacgtcgt tctgttgctt
     1201 tcatacacat tcctttcgta cacttttttc cattatatca aaattcctat aaatcaacct
     1261 ttcctcaacc aaaaaacgga aactgcagaa cagtctccgt tatcatctgc tagtttagca
     1321 atgcttgaat agccgcaatg tccaaacgct tcatggtcat caccgctgca ttgactgcct
     1381 tgaccttttc tggattagcc gtatcgtatg cctccatcaa aatacgcggc acgatctgcc
     1441 aagaaatccc aaactggtcc ttggcccaac cgcaaatctc cgcttctggc acagcagaca
     1501 ctttttcata ccagctctcc gcttcttcct gactgtctac atagaccatg aatgagaagg
     1561 cttcgttgaa agaagccaca ccaccgtagc cgtggtccat agcgataatt ttttggtctc
     1621 cgataaaaag ctccgcataa ttcaacttgg cagccgccac cgaagcctca ccttcgccat
     1681 agtaagacaa gcgaccaaga cgagcttgat gcagcttatc cttgtagtaa tccaacatag
     1741 gctgggccaa accaacctgt tcctgcgaga agagcaggca gatgtcaaac tgataaggct
     1801 tgtccaagtc cggtgcataa gacagttgcc aagacagacc aaagcggtct tccaaccaga
     1861 catagtaagg gctgaaagga cattctccca aaggcatgag cacacgaccg ccttctgaca
     1921 agacttggta caaacgtgtc acttcatcct tgctagctac attgaccatg agggacatgg
     1981 actcattgag cttaaagtat ggacccgcac taatagccgc caaggtcata tcgcccagtt
     2041 gaaactgaat caaatccgaa tcacccgaag gcgtatccgt aatggtgtag gtccagtcaa
     2101 tcttgccacc gaagagttct acatagaaag ccaccgcttc ttttgcctct gtatcatacc
     2161 agagatgtgg gattatagtt tgcattgatt tctcctttga gttagtagcc ccagtataac
     2221 acttgtccgt acaaaaacaa aacatgttgc tttgcactta cagatgtgct atactgaata
     2281 actacaaaat tgacaggaga atgaaatgga aatctggaac acctacacag ccgacggtca
     2341 cttgaccgac cacaccctca cacgcggaga atccattccc gacggcctct accacctggt
     2401 cgtcgagtgc atcatccgcc accacgacgg cagtaccctc ttcatgaaac gcgacagcac
     2461 caagccctcc taccctgact attatgaagc aacagcagga ggttcagctt tgtttgggga
     2521 aatagcagag caggccattt tgcgcgaagt ccgcgaagag acaggcattg agctgacagc
     2581 tgaccaactc cgccaccata cacactttgt cgcccatgac gaccagtgca ttttccactg
     2641 ctactgggct gaaaccgact gggacaaatc cacaatccaa ctccaatcca acgaaaccag
     2701 caattatatc tgggtcccac aagaaaattt gaaaaatttc ctggaaacag agctagtcat
     2761 tccaaggcag aaagactatg tggagaggtt gtttttaaaa agctaacccc ccccttaaca
     2821 gacaaatccc cactatcaca ggacagcgga gattatttca ttacaaactc ttcaacatat
     2881 tgtcaatatg ctggatggat ttctcacggc cgagcaggta aatggtgttt ggcaactctg
     2941 gtccgtgcat ttcgcctgaa acggcgatac ggattggcat gaagaggttt ttgcccttga
     3001 tgcctgtttc tttttggaca gccttgattt gcgggaagat gttatctggt tggaaatctt
     3061 cgtccgtcat ggcttccaat ttttccttga gggcattgag aacagtcggt actgtttcgc
     3121 ctgccatcac ttctttttct tccgcagtca attctgggaa atctgagaag aagaggtctg
     3181 tcaaaccaac gatttcatcc gcagatgaca gttgtggctt gtaaagttcc accaattttt
     3241 ctgctttgtc tgtcaaacgc cccgcttctt ccaagaatgg cttagcaagg ttgaaaatag
     3301 tttctaaatc agcatttttg atgtactcat tgctcatcca gtccattttc ttctggtcaa
     3361 aggcagctgg cgatttgctg aggcggttct cgtcaaagag ttggataaat tgctcacggg
     3421 agaagatttc ttcctcgcca cccgggttcc aacccaaaag accgataaag ttgaaaacag
     3481 cttcaggcat gtaaccttta cgacggtaat cttctatgaa ctgaagggtg ttagtatcac
     3541 gtttagacag ttttttacca gtttcagaat tgataataag cgtcatgtga ccaaattctg
     3601 gtgcttccca accaagtgct tcgtagacca tgagctgctt gggtgtattg gcaatgtggt
     3661 cgtccccacg gataacgtgc gagattttca tgagataatc gtccaccacc acggcaaagt
     3721 tgtaagttgg atagccgtca cgtttctgga taacccagtc accaccgatg ttgccacctt
     3781 cgaactcgat ttcgccctta accatgtcat tccacttgta gatacctgtt tcgttgacag
     3841 ccaagcgaac cgttgggaca atgccagccg cctcacgctc tgcgatgtag gccgcttttt
     3901 catcttcaga cataccaagg tattcgttga tgtagcgagg agtttcaccc gcagcttcct
     3961 gacgctcacg ctccgccgcc aactcttctt cggtcacata agacttgtag gccttgcctt
     4021 ctgccaagag ctgatcaaca tacttttggt aaatatccaa acgctcagac tgacggtatt
     4081 gctcatgagt ttctggactc tcatcccagt caatccccaa ccaacgcagg ttttcaagct
     4141 gagaacgctc gccgtcttcc acatggcgct tgcggtctgt gtcctcgata cgaataagga
     4201 aagtaccgcc gtgatgacgg gcaaaaagat agttaaacaa tgctgtacga gcatttccga
     4261 tatgcaaaag tcccgttgga cttggtgcgt aacgcacacg aattggttta gtcatgtttc
     4321 tctcctgtaa aatttcaatc aagaacatta tagcatagaa cacccccaga taacagaaaa
     4381 atcagtctaa acagactgat tttgaggtgt tttttggtta cgtgccagcc agtagaggat
     4441 atagaggact aacaaaccag ttgcagctag gaaaagtccg ctaatctgat tgacagtcaa
     4501 gattaagacc aaacccgaaa ccaacagtcg agtaaaggct tgacctagca taaaataggt
     4561 tgataccgct ccgccaatgg tcgccaactg ctcctctggc atagaattga caaacttagc
     4621 gttaaactta ggtccactag ctcctgaact aatccccatt gtagtaagga agaagaaaat
     4681 aattggcagt tgatgtagca acattccgac aaaaacgcca agtagagaga aggttgctgc
     4741 cacttccagc gtcaccatgc tggtattttt gaaaagggta aagaccaagg tcgagcccag
     4801 aatatgtccg ataaagaaga tcagaatggt cagggcaagg gttgtttcaa cattgaggaa
     4861 gacaaggcct gggtcttcgc taatcagcag gaccagaata ggatagagaa tcgctccaca
     4921 ggcgttgata agaggggtgg taatcaggac caggcggaac tcgggtatct tgcgcagctc
     4981 cacgatggct tgcttgaagg aagctagaat gcctggtctc ttggtttttt gctcagtctg
     5041 gctcgactgc tctgctattt tcaaaggctg ctccgtcagg agtttgcgaa agactggcgt
     5101 cagcagctgc atgatgagga gggcggctaa aaaggtgccg gcattgagaa aggccaggtt
     5161 ttggtaggag aggaggccga ccaagagggc agagagggcc tgaaaggcga tattgaggag
     5221 gctggtcacg gtctggcgaa aggccgagta ctgctcccgc tcttctttgg gcgctacgcg
     5281 taatcccaga ggtgtataga ggccgttttc atagaagcca gcgaagtctg caaagacatt
     5341 gaaagttgca gccaccacga caatccagag ggctggctca aagcccatgc agaatccgag
     5401 gatgaggtat agaagaacac gaaagagtag ggtcagcttg atagtgctga ttttgttgac
     5461 cgtcttgtct gccagatagc ctgtaaagag ccccataaag ctagggaaga tttccgagaa
     5521 ggtcacaatc gccagagcca gacgactgtt gggcaccagc aaaacatagt tcatcaaggc
     5581 cagataatag accacatcgc caaaattgga cagcatatcc gccgtcagta cccataggta
     5641 ggtagcattt ttcaaaatct ttttcatgat aatccctttc ttttgaataa tttccagaat
     5701 attccgaatt tttattttac agaggaacct ggatagactt tcc
//
LOCUS       DAZQCJ010000111         5391 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000111 DAZQCJ010000000
VERSION     DAZQCJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.112"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(102..344)
                     /locus_tag="ACHU66_001879"
     CDS             complement(102..344)
                     /locus_tag="ACHU66_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFR3390143.1"
                     /translation="MKKDIHPEYRTVVFMDTTTGYKFLSGSTKKSNETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(463..1491)
                     /locus_tag="ACHU66_001880"
     CDS             complement(463..1491)
                     /locus_tag="ACHU66_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFR3390144.1"
                     /translation="MQASSRSLRLMGTIIETRIWYPQAETILDQVEELLYLYKDRFSA
                     NDLTSELMEVNLNAGVQAVPVADDLYELIKLGKEHSLAQGSFLNITIGPLVQSWRIGF
                     SDAKLPTQEMISEKLQLINPRDIELDDEEQTVYLKKEGMAIDLGALAKGYVADKIVDF
                     LKRIGVEAGLINLGGNVLTFGQAPHNADGYWRIGIQDPQKLRGENALVLKIGEESVVT
                     SGIYERTLTVNGKTYHHILSPETGYPMESQLASLTIVSKQSVDGEIWTTRLFGQEITR
                     IYKMVEETDGIEAVLIGLDGRILVTSGLSEKVLAGEERISDSLGSPSRGGFGARVTSD
                     LASGASVL"
     gene            complement(1478..2422)
                     /locus_tag="ACHU66_001881"
     CDS             complement(1478..2422)
                     /locus_tag="ACHU66_001881"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFR3390145.1"
                     /translation="MTIYSKIIKKIHEYDTIIIHRHKRPDPDAIGSQVGLQKLLQKNF
                     PEKTIKVTGFEEPNLAWMAGMDVVADQDYQGALVIVTDTANTARVDDDRYTQGDFLIK
                     IDHHPNEEPYGDLLWVNTEASSCSEMITELAIQSQLELDGDIARLLYAGIVGDTGRFL
                     YPATSSRTFEIVARLRQEDFDFARMSRQMDTIDFKIAKLTGYVYDNLEVDEHGAARVI
                     LTQDILEKYGVTDADTSFIVGAPGRIGIVQSWGIFVEQTDGNYRVRLRSKYLPINEIA
                     KRHDGGGHPLASGANSYSLSENEQIYQEIQEVVRNASK"
     gene            2517..2978
                     /locus_tag="ACHU66_001882"
     CDS             2517..2978
                     /locus_tag="ACHU66_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR3390146.1"
                     /translation="MALAKIVYASMTGNTEEIADIVASKLEELGLEVQVHECTTIETE
                     EILDADLIVVASYTYSYGGDGELPDEIVDFYADLADLDLTGKVYGVCGSGDTFYDDFC
                     SAVDDFDVMLGSRGATKGAENVKVDLAAEDEDIVNLEQFATDLVAKLDTMN"
     gene            3118..3387
                     /gene="rpsN"
                     /locus_tag="ACHU66_001883"
     CDS             3118..3387
                     /gene="rpsN"
                     /locus_tag="ACHU66_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR3390147.1"
                     /translation="MAKKSKMARYQRQLELIERYADLRKSLKEKGDYQALRKLPRDSN
                     PNRLKYRDRTDGRPHAYMRKFGVSRITFRELAHLGQLPGVKKASW"
     gene            3469..3747
                     /locus_tag="ACHU66_001884"
     CDS             3469..3747
                     /locus_tag="ACHU66_001884"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFR3390148.1"
                     /translation="MNLDCIREQINTIDSQLVELLENRMELVDQVTAYKRATGKPVLD
                     TSRENAVLERVGKLVQKDDYRSAIQATFSDIMAQSRTYQSSKLANHEQ"
     gene            3737..4951
                     /locus_tag="ACHU66_001885"
     CDS             3737..4951
                     /locus_tag="ACHU66_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFR3390149.1"
                     /translation="MSNKMKQAIQLLFFSNLIGVGAGIITTLFGKILLGIGDLRSEYF
                     TFLIPFLPLAGGVIVFIYQKWGREVQAGMGLVFKAGQGEKAQISPVLIPLIISTTWLS
                     HLVGASVGREGVAVQLGASLSHWLKKHGFTHLPKDMITKIGMAAGFAGLFQTPLAASF
                     FAIEVLIVGQYSWTSLPYCLVAAFTASTTSHLLGLEKFSHTVATTSFYFTDSFKWLFI
                     ALCFGFIGNLFAWFLAQAKSISTRWLPNPYIKIAIMGVGLTVLLFFFHQGHYTGLGTN
                     LIDASLAGEQVFAFDWLLKLLLTCLCLAAGFQGGEVTPLFAIGASSGVVLAGLLGLPT
                     ELVAALGYCAVFGTATNTLLAPLFISYEVFGANILPYAIPVLAIAYLINRKQTIYGQQ
                     LRKFNNAKKPII"
BASE COUNT         1467 a         1156 c         1102 g         1666 t
ORIGIN      
        1 ctcttttatt tctataggca aagaaaaaat ggtccactgg accataactc gctcttttat
       61 tttcaagctc gtgaaaaaac agtccgttaa ggactgtttt tttatttgag accgtatttt
      121 ttgttgaagc gatccacacg tccatctgct tgagtgaact tttgacgtcc agtatagaat
      181 gggtgtgagt ctgatgaaat ttccacacgg atcaatgggt aagtttcacc ttcgaattca
      241 actgtttcgt tagatttctt agttgaaccg ctaaggaact tgtagccagt agttgtgtcc
      301 atgaagacaa cagtgcgata ttctggatgg atatcttttt tcattttaaa attcctttct
      361 gccatggact ctttccgagc catagattat aaccatttaa gtttaccaaa agtttgttct
      421 tatggcaagt ctttttacct atttttttaa tattttttca agttatagta cggaagcacc
      481 tgaagcaagg tcagaagtta ctctggcgcc aaatccccct cttgaaggac tgcccaagct
      541 atcagaaatt ctttcttcac cagcaagaac tttctcagaa agccctgagg tcactagaat
      601 cctgccatcc agcccaatca gaaccgcttc gataccatca gtttcttcta ccatcttata
      661 aatccgagtt atttcttgac caaacagtcg agtcgtccaa atctcaccgt caacagattg
      721 tttggacaca atggtcaaac tagccagctg cgactccatt ggatagccgg tctcaggact
      781 caaaatatga tgataggtct ttccattcac agtaagggtt ctttcgtaaa taccagaggt
      841 gactactgat tcttctccta tttttaggac gagggcattt tcaccacgta gtttctgagg
      901 atcctgtata ccaattcgcc aatagccatc tgcattgtga ggagcttgac caaaggtcag
      961 gacgttgcca cccagattaa tcagtccagc ctccacacct attcttttca agaagtccac
     1021 aatcttatct gctacatatc ctttagcaag agcacccaga tcaatcgcca taccttcttt
     1081 tttcaaatag actgtctgct cctcatcatc taattcgatg tcccttggat tgatgagctg
     1141 gagtttttca ctgatcattt cttgggttgg aagtttggcg tcgctaaatc caatccgcca
     1201 gctttgtaca aggggaccaa tggtaatatt caaaaaagat ccctgagcca gactgtgttc
     1261 tttgcccaat ttaatcaatt catacaggtc atcggcaaca ggaaccgcct gaacacccgc
     1321 attgaggttg acctccatta attcagaggt caagtcattg gcagaaaacc gatccttgta
     1381 gagatagaga agctcttcta cctgatccaa aatcgtttct gcctgaggat accatattct
     1441 ggtttcaatg atggttccca taagacgaag ggagcgacta cttgcttgca ttcctcacta
     1501 cctcttggat ttcttggtat atttgctcat tttcggagag tgaataagag ttagcaccac
     1561 ttgctagtgg gtggccaccg ccatcatggc gtttggcaat ctcgttgatt ggaagatact
     1621 tactgcgcaa acggactctg taatttccgt cagtttgttc cacaaaaatc ccccaagatt
     1681 gaacgattcc aatccgtcct ggcgcaccta cgataaagga agtgtccgca tcggtcaccc
     1741 cgtatttttc gagaatgtcc tgtgtcaaaa taacacgcgc cgcaccatgt tcatccactt
     1801 ccaagttgtc gtagacgtaa cctgtcaact tggcaatctt gaaatcgatg gtatccatct
     1861 ggcgagacat ccttgcaaag tcaaaatctt cttggcggag acgagcaaca atctcaaagg
     1921 tgcgcgagct ggttgccgga tagagaaagc gacctgtatc gcccacaatt cctgcataga
     1981 gtagacgagc aatatctcca tcgagttcca gttggctttg gatggctagt tccgtaatca
     2041 tttcactgca tgagctggct tccgtattga cccagagcag gtcaccataa ggctcttcat
     2101 tggggtgatg atcgatttta atcaagaaat ctccttgggt atagcggtcg tcatccacac
     2161 gcgccgtatt ggcagtgtct gtcacgataa ccaaggctcc ttgataatct tggtctgcaa
     2221 ctacgtccat tcctgccatc caagcaagat ttggttcctc aaaaccggtt accttgatgg
     2281 ttttttcagg aaaattcttc tgcaagagtt tctgcaagcc aacttgacta ccaatggcat
     2341 ccggatctgg tcgtttatgt cggtgaataa taatcgtgtc gtattcgtga atttttttaa
     2401 taattttact gtaaattgtc atagcaactc cttttcatca ttttagcata aacttcccct
     2461 tttgaaaatg atgggagtgt ggtataatgg gattactatg taatttggag gaaattatgg
     2521 cactagcaaa aatagtttac gctagtatga ctggtaacac agaagaaatt gctgacatcg
     2581 tcgccagtaa gctagaagag ttaggtttgg aagtacaggt tcacgaatgt acgactattg
     2641 aaaccgaaga aatcttggat gcggacctca tcgtggtagc cagctacacc tattcttacg
     2701 gtggagacgg tgagcttcca gatgaaattg ttgatttcta tgctgaccta gctgacttag
     2761 atttgaccgg taaggtctac ggtgtttgtg gttctggcga taccttctat gatgacttct
     2821 gtagtgcagt agatgacttt gatgtcatgt tgggttcacg tggtgcaact aagggtgccg
     2881 aaaatgttaa ggtagatctt gccgctgaag atgaggatat tgttaacctt gagcaattcg
     2941 cgacagacct tgttgctaaa ctagatacaa tgaattaact gacaaacaga ctggcttatc
     3001 aataggtcta gtctgttttc ttttagcctt ttattaagat tgtacaccta ttgcttgcaa
     3061 tcttaatagt tttgatatat aattaactgg ttaagttttt tgaaaaagga gaattttatg
     3121 gctaaaaaat caaaaatggc acgctatcag agacagttgg agttaatcga gcgctatgcg
     3181 gatttacgta aaagtctcaa agaaaaaggt gactatcaag cacttcgaaa attgcctcgc
     3241 gactcaaatc caaatcgact gaaataccgc gacaggaccg atggccgtcc gcatgcttat
     3301 atgcgaaaat tcggtgtgag tcggattacc ttccgtgaat tagcccatct tggtcaactt
     3361 cctggggtta aaaaagcaag ttggtaactt cctcaaacaa ggctagtatt ctgccttgtt
     3421 ttttgctata ctaaaaaaca actgaaacaa tatacggagg tttataagat gaatttggat
     3481 tgcatccgcg aacagattaa cacgattgat agccaattgg ttgaactact tgaaaatcga
     3541 atggagttgg tcgaccaagt tacagcctat aaacgagcaa caggtaagcc tgttttggat
     3601 accagccgag aaaatgctgt ccttgaaaga gttggaaaat tagttcaaaa ggatgattac
     3661 cgttctgcca ttcaagcaac ctttagcgat attatggccc aatcaagaac ctatcaatct
     3721 tccaagttag cgaatcatga gcaataagat gaaacaagcc atccaactcc ttttcttttc
     3781 caacttaatt ggagtcggtg caggaataat caccacactt tttgggaaaa ttctactagg
     3841 gattggagac ctacggtctg aatattttac ttttctaatc ccctttctgc cacttgcagg
     3901 gggggtgatt gttttcattt atcaaaaatg gggaagggaa gtccaagcag gtatgggctt
     3961 agtcttcaaa gctggacaag gggagaaagc gcaaatttct ccagttctca ttccactcat
     4021 catctctaca acttggctca gtcatcttgt cggtgcttcc gttggtcgcg aaggtgtagc
     4081 tgtccaactt ggagccagtc tctcacattg gttaaaaaaa catggtttca cacacttgcc
     4141 caaagacatg ataacaaaga ttggtatggc cgcaggtttt gcagggctat ttcaaacacc
     4201 gttggctgct agtttctttg ccattgaggt attaatcgtt ggccaatatt cttggactag
     4261 tctcccctat tgtctagttg ccgctttcac ggcttctact acttcccatt tattgggatt
     4321 agagaaattt tcgcataccg tcgctactac ctcattctat tttacagata gttttaaatg
     4381 gctatttatt gccctttgct tcggtttcat cggcaattta tttgcttggt ttctggcgca
     4441 agcaaaaagc atctcaactc gatggcttcc aaatccttac attaaaatag ctatcatggg
     4501 agttggactg actgttctac tatttttctt ccatcaaggt cattatactg gtctaggaac
     4561 caatttgatc gatgccagtt tagctgggga acaggtgttt gctttcgatt ggctactaaa
     4621 actcctacta acctgtcttt gtttggctgc aggttttcaa gggggcgaag ttactccgct
     4681 ctttgcgatt ggagcaagtt ctggggttgt tttagcagga ttactaggtc taccaactga
     4741 acttgttgca gcccttgggt attgtgccgt atttggaaca gccaccaata ccttactggc
     4801 tcctctcttc attagttatg aagtattcgg cgctaatatc ctgccctatg ctattcctgt
     4861 acttgccata gcttatctta taaacagaaa acagactatt tatggacagc agttgaggaa
     4921 attcaataat gcaaaaaagc ctatcatcta ggctttttcg tatactcttc atctcccctg
     4981 caggaatttt atagcaaaac acgttgttgt atgttttaaa aaatacgctg attttagcta
     5041 tttttgttat atttttgacc tatctatact cattttttaa aacttttggg gcgaatttgg
     5101 ggcagacgca ctaaaaaaac actccataaa tcaacttctc aagacctctg gacaaggtct
     5161 ttttttgtcc gcaaaaacgg caatttttaa aaacgtccga gataaaagta agtatttttc
     5221 tatcaaattt tgaaaaaacg ctattttttt gaaattcggg taacaaaaaa agcccccagc
     5281 gtttgctgag gggtttttga aatacaatga tagcggttgg gcggcgtatc caacatctca
     5341 ggtactctct cgagtgcgtc aggagccttt ctgacctcgt tatcattcat t
//
LOCUS       DAZQCJ010000112         5338 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000112 DAZQCJ010000000
VERSION     DAZQCJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.113"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            174..1722
                     /locus_tag="ACHU66_001886"
     rRNA            174..1722
                     /locus_tag="ACHU66_001886"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1776..1848
                     /locus_tag="ACHU66_001887"
     tRNA            1776..1848
                     /locus_tag="ACHU66_001887"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1809..1811,aa:Ala,seq:tgc)
     gene            2151..5054
                     /locus_tag="ACHU66_001888"
     rRNA            2151..5054
                     /locus_tag="ACHU66_001888"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5243..>5338
                     /gene="rrf"
                     /locus_tag="ACHU66_001889"
     rRNA            5243..>5338
                     /gene="rrf"
                     /locus_tag="ACHU66_001889"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1507 a         1099 c         1525 g         1207 t
ORIGIN      
        1 caagaaatga taaactaaga tagttgtcgc gagaacgtga taacgacaag acctttgaaa
       61 attaaagaag acgaaccaaa cgtgcagggt gatttatcta aggataaatc gtcaatgaca
      121 aaacaaaaca ataaaacgga aagctagcaa tagcttgagt ttgaatcaaa actttttatg
      181 agagtttgat cctggctcag gacgaacgct ggcggcgtgc ctaatacatg caagtagaac
      241 gctgaagtct ggtgcttgca ctagacggat gagttgcgaa cgggtgagta acgcgtaggt
      301 aacctgcctc atagcggggg ataactattg gaaacgatag ctaataccgc ataacagtat
      361 ttaccgcatg gtagatgctt gaaaggagca actgcttcac tatgagatgg acctgcgttg
      421 tattagctag ttggtgaggt aacggctcac caaggcttcg atacatagcc gacctgagag
      481 ggtgatcggc cacactggga ctgagacacg gcccagactc ctacgggagg cagcagtagg
      541 gaatcttcgg caatgggggc aaccctgacc gagcaacgcc gcgtgagtga agaaggtttt
      601 cggatcgtaa agctctgttg taagagaaga actgtgagaa gagtggaaag tttctcactt
      661 gacggtatct taccagaaag ggacggctaa ctacgtgcca gcagccgcgg taatacgtag
      721 gtcccgagcg ttgtccggat ttattgggcg taaagcgagc gcaggcggtt tgataagtct
      781 gaagtaaaag gctgtggctt aaccatagta cgctttggaa actgtcaaac ttgagtgcag
      841 aaggggagag tggaattcca tgtgtagcgg tgaaatgcgt agatatatgg aggaacaccg
      901 gtggcgaaag cggctctctg gtctgtaact gacgctgagg ctcgaaagcg tggggagcga
      961 acaggattag ataccctggt agtccacgcc gtaaacgatg agtgctaggt gttgggtcct
     1021 ttccgggact cagtgccgca gctaacgcat taagcactcc gcctggggag tacgaccgca
     1081 aggttgaaac tcaaaggaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat
     1141 tcgaagcaac gcgaagaacc ttaccaggtc ttgacatccc gatgaccgcc ctagagatgg
     1201 ggtttctctt cggagcatcg gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga
     1261 gatgttgggt taagtcccgc aacgagcgca acccctattg ttagttgcca tcattcagtt
     1321 gggcactcta gcgagactgc cggtaataaa ccggaggaag gtggggatga cgtcaaatca
     1381 tcatgcccct tatgacctgg gctacacacg tgctacaatg gctggtacaa cgagtcgcaa
     1441 gtcggtgacg gcaagctaat ctcttaaagc cagtctcagt tcggattgta ggctgcaact
     1501 cgcctacatg aagtcggaat cgctagtaat cgcggatcag cacgccgcgg tgaatacgtt
     1561 cccgggcctt gtacacaccg cccgtcacac cacgagagtt tgtaacaccc gaagtcggtg
     1621 aggtaacctt ttaggagcca gccgcctaag gtgggataga tgattggggt gaagtcgtaa
     1681 caaggtagcc gtatcggaag gtgcggctgg atcacctcct ttctaaggaa atggaaacct
     1741 gtacgtcagt cttctttaat tttgagaggt cttgtggggc cttagctcag ctgggagagc
     1801 gcctgctttg cacgcaggag gtcagcggtt cgatcccgct aggctccatt aggatagaaa
     1861 tcctactaaa ctgaataaca gtgaagttga attcacgact aacttcttag gaaaatagat
     1921 aatcttcctt gtgtgcaaga cacacaatgt cagattccta attttctaca gaagtttcgc
     1981 agaagcgacc gtcgttcata tcctaaactt gtccattgaa aattgaatat ctatcaaaca
     2041 ttcctaaacg tatgtaaaaa tacgtataga aatagtaaca agaaaataaa ccgaaaacgc
     2101 tgtgaatatt taatgagttt tctaattttt gaaaaaaatt aggttaataa ggttaagtta
     2161 ataagggcgc acggtggatg ccttggcact agaagccgat gaaggacgtg actaacgacg
     2221 aaatgccttg gggagctgta agtaagcaat gttccaggga tgtccgaatg ggggaacccg
     2281 gcaggtaatg cctgtcactc actactgtta aggtagtgaa gaggaagacg cagtgaactg
     2341 aaacatctaa gtagctgcag gaagagaaag caaaagcgat tgccttagta gcggcgagcg
     2401 aaacggcagg agggcaaacc gaggagttta ctcctcgggg ttgtaggact gcaatgtgga
     2461 cttaaagagt atagaagaac tacctgggaa ggtaggccaa agagagtaat agcctcgtat
     2521 ttgaaatatt ctttatacct agcagtatcc tgagtacggc gagacacgcg aaatctcgtc
     2581 ggaatccggg aggaccatct cccaacccta aatactctct agtgaccgat agtgaaccag
     2641 taccgtgagg gaaaggtgaa aagtaccccg gaaggggagt gaaatagaac ctgaaaccgt
     2701 gtgcctacaa caagttcgag cccgttaatg ggtgagagcg tgccttttgt agaatgaacc
     2761 ggcgagttac gatatgatgc gaggttaagt tgaagagacg gagccgtagg gaaaccgagt
     2821 cttaataggg cggattagta ttatgtcgta gacccgaaac catgtgacct acccatgagc
     2881 aggttgaagg tgcggtaaga cgcactggag gaccgaacca gggcacgttg aaaagtgctt
     2941 ggatgacttg tgggtagcgg agaaattcca aacgaacttg gagatagctg gttctctccg
     3001 aaatagcttt agggctagcg tcgacatttg agaatcttgg aggtagagca ctgtttggat
     3061 gagggggcca tctcggttta ctgatttcag ataaactccg aatgccaatg atttatggtc
     3121 ggcagtcaga ctgcgagtgc taagatccgt agtcgaaagg gaaacagccc agaccaccag
     3181 ctaaggtccc aaaataattg ttaagtggaa aaggatgtgg ggttgcacag acaactagga
     3241 tgttagctta gaagcagcta ttcattcaaa gagtgcgtaa tagctcacta gtcgagtgac
     3301 cctgcgccga aaatgtaccg gggctaaaac aatttaccga agctgtggat aacactttag
     3361 tgttatggta ggagagcgtt ctatgtgtga agaaggtata ccgtgaggag tgctggaacg
     3421 catagaagtg agaatgccgg tatgagtagc gaaagatggg tgagaatccc atccaccgta
     3481 agactaaggt ttccagggga aggctcgtcc gccctgggtt agtcgggacc taaggagaga
     3541 ccgaagggtg tatccgatgg acaacaggtt gatattcctg tactagagta tgaagtgatg
     3601 gagggacgca gtaggctaac taaagcgggc gattggaagt gcccgtctaa gcagtgaggt
     3661 gtgatatgag tcaaatgctt gtatctgtaa cattgagctg tgatggggag tgaagttaag
     3721 tagcgaagtt agtgacgtca cactgccgag aaaagcttct agcgatgtat catactctac
     3781 ccgtaccgca aaccgacaca ggtagtcgag gcgagtagcc tcaggtgagc gagagaactc
     3841 tcgttaagga actcggcaaa atgaccccgt aacttcggga gaaggggtgc tggcttaaag
     3901 tcagccgcag tgaataggcc caagcaactg tttatcaaaa acacagctct ctgctaaatc
     3961 gtaagatgat gtataggggg tgacgcctgc ccggtgctgg aaggttaaga ggagggttta
     4021 gcgcaagcga agatctgaat tgaagcccca gtaaacggcg gccgtaacta taacggtcct
     4081 aaggtagcga aattccttgt cgggtaagtt ccgacccgca cgaaaggcgt aatgatttgg
     4141 gcactgtctc aacgagagac tcggtgaaat tttagtacct gtgaagatgc aggttacccg
     4201 cgacaggacg gaaagacccc atggagcttt actgcagttt gatattgagt atctgtacca
     4261 catgtacagg ataggtagga gcctacgaag tcgggacgcc agtttcgacg gaggcgctgt
     4321 tgggatacta cccttgtgtt atggctactc taacccggat aggttatccc tatcggagac
     4381 agtgtctgac gggcagtttg actggggcgg tcgcctccta aaaggtaacg gaggcgccca
     4441 aaggttccct cagaatggtt ggaaatcatt cgcagagtgt aaaggtataa gggagcttga
     4501 ctgcgagagc tacaactcga gcagggacga aagtcgggct tagtgatccg gtggttccgt
     4561 atggaagggc catcgctcaa cggataaaag ctaccctggg gataacaggc ttatctcccc
     4621 caagagttca catcgacggg gaggtttggc acctcgatgt cggctcgtcg catcctgggg
     4681 ctgtagtcgg tcccaagggt tgggctgttc gcccattaaa gcggcacgcg agctgggttc
     4741 agaacgtcgt gagacagttc ggtccctatc cgtcgcgggc gtaggaaatt tgagaggatc
     4801 tgctcctagt acgagaggac cagagtggac ttaccgctgg tgtaccagtt gtcttgccaa
     4861 aggcatcgct gggtagctat gtagggacgg gataaacgct gaaagcatct aagtgtgaaa
     4921 cccacctcaa gatgagattt cccataactt tatgttagta agagccctga gagatgatca
     4981 ggtagatagg ttggaagtgg aagtgtggcg acacatgtag cggaccaata ctaatcgctc
     5041 gaggacttat ccaaactaag taacgaaggc gtcaaatccg cctgaaaaat aggaacctaa
     5101 cgcagtatgc ttgcatacaa ggcaggttat cttttttcca aggatttagc ccgagtacaa
     5161 ttactcacga gtcaatattg acaagcgatc ggtttcttgt tagaatatag atattcaatt
     5221 ttgagttgac aagactcatt agttaagtga cgatagccta ggagatacac ctgtacccat
     5281 gccgaacaca gcagttaagc cctagaacgc ctgaagtagt tgggggttgc cccctgtt
//
LOCUS       DAZQCJ010000113         5276 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000113 DAZQCJ010000000
VERSION     DAZQCJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.114"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            255..1412
                     /locus_tag="ACHU66_001890"
     CDS             255..1412
                     /locus_tag="ACHU66_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR3390150.1"
                     /translation="MLKTKKAKIALFGGLGVVAVALVGGALIFSNMLTSPSSSEDVAM
                     SMTYTVAKEGSIASSTLLTGTITAADEQYVYYDPSKGDLSEVLVAPGAKVEVGTPLIR
                     YDATELQSALDTAVRGRDKIGRQIADLRTNGQTVQTTGDAETDAAATAAAQRSVDMQL
                     ADLNDSYADAQAAVDKAYTALTEATVTSTVAGTVVEVNKAVSKNSTSSQTVVHIVNQG
                     SLQVTGSLTEYDLANIAVDQEVKLTSKVYPDKTWTGKITYISNYPSSNQAGAATTGTS
                     GSGAKYPFKAALTSELGELKQGFSVNIEVVNTSKNILIPVSAVVPEEDKNYVWTLVDG
                     KAKKVEVTLGNADALNQEVTAGIAAGDKVITIPTPNLEDGKEVEANEEPAY"
     gene            1393..2088
                     /locus_tag="ACHU66_001891"
     CDS             1393..2088
                     /locus_tag="ACHU66_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3390151.1"
                     /translation="MKNQLIRLTSINKSYKNGDQELRVLKNIDLEVEEGEFLAIMGPS
                     GSGKSTLMNIIGLLDRFSSGNYWLEGEEVSQLSEKKLAQVRNDQIGFVFQQFFLLSKL
                     NALQNVELPLIYAGVPSSKRKELAKQYLEKVELAERMTHLPSELSGGQKQRVAIARAL
                     VNTPAIILADEPTGALDTKTGEQIMQLLTELNNEGKTIIMVTHEPEIAAYAKRKIVLR
                     DGVITEDSRREGV"
     gene            2089..3336
                     /locus_tag="ACHU66_001892"
     CDS             2089..3336
                     /locus_tag="ACHU66_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR3390152.1"
                     /translation="MENWKFALKSIMAHKMRSLLTMLGIIIGVAAVVIIVAMGTGVAK
                     SIEKSLAGDQNNVQVYFTAFSSSGTAFGPFGTASTEEPILEEEPDLTDSTLKGLLEID
                     GVSNYYSSISNIAEVRSGNKKAENVTITGVSQNFFAVKEYEILAGRQFTANDYSRFSR
                     MIMLDTALAEKLFGSIENSLNQTISVNSNAYLVIGVYKDPKAGTAIYGMNSGGNAVMT
                     NTQLAAETGMKENSQIFVHVEDVTRATEVGKAAADYLTKATGLKEARYEIYDMSAMLE
                     EFKSQMSGVTLFIGAVAGISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRGNILI
                     QFLIEAMVLTTLGGAIGLAIAQTIVFLLNVSKALGERIAAEISIPVVLGSLAFSAVVG
                     IVFGVLPANKASKLDPIEALRYE"
     gene            3462..4526
                     /locus_tag="ACHU66_001893"
     CDS             3462..4526
                     /locus_tag="ACHU66_001893"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR3390153.1"
                     /translation="MVELGISTFGETTPLEKTGETYSHDERIHQLVKEIELADAVGLD
                     VYGIGEHHREDFAVSAPEIVLAAGAVNTKHIKLTSAVSILSSMDPVRLYQQYTTIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLHDYEELFDEKLDMLLEIDKETNLKWDGHFTQSV
                     DNKPVYPRPVQEDFPIWVATGGNVESTIKIAKKGLPIVYAVIGAGAHRFKPLVDAYRK
                     VARNSGHDPKKTKVAAHSWGWIADDHDKAVEEYYHPTKVITDNIAKDRPHWSEMTKAQ
                     YLYSLTDEGATIVGDPKRVAEKIIKTIETLDLDRFFLHLPIGSMPHEDVLRAIELYGK
                     EVAPIVRDYFAKKEENQSQD"
     gene            4910..>5276
                     /locus_tag="ACHU66_001894"
     CDS             4910..>5276
                     /locus_tag="ACHU66_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFR3390154.1"
                     /translation="MENIFAIKNKILDFPFLEEVNEDTTINKCGIYMLYIDFFGDNSI
                     LPIYIGKSKDVQKRFDSHLKKIMFLNRLSESTYLSYFFEREDPFYDGSFFYCKIFQYM
                     IDHQCTLTDFKMKVVEFCSL"
BASE COUNT         1635 a          937 c         1171 g         1533 t
ORIGIN      
        1 caaattgaac aaattattct agctcataat ttacgctatt ttgaaatgaa ctagcaccac
       61 gggttaacta tattcattgt aacgcttttt tgtgtgtcaa tcaaaaatcc gctacgttac
      121 cttacaaaaa tgtaagtggg aaaattgccg aactttttat aaaatagtta ggaaaaaact
      181 tgcatttgtt cttgtatttg tgtattatat agacaacaca aataggtatg ttttgtaaag
      241 aaaaggagtt tgttatgtta aagacgaaga aagcaaaaat tgctttgttt ggtggtcttg
      301 gggtagtcgc agttgcctta gtaggtgggg ctttgatttt tagcaatatg ctaacatcac
      361 catcatcttc agaagatgta gccatgagta tgacctatac tgttgctaaa gaaggatcaa
      421 ttgcttcctc aacgctactt acaggaacga ttacagcagc agacgagcag tatgtttact
      481 atgatccttc taagggagat ttgagtgagg ttttggttgc tccaggtgct aaggtcgaag
      541 taggtacccc tttgattcgc tatgatgcta cagaattaca gtctgctttg gatactgctg
      601 ttcgtggtcg tgataagatt ggtcgccaaa ttgcagactt aagaaccaac ggtcagactg
      661 ttcaaacaac tggtgatgca gaaacagatg ctgcagcaac tgctgctgca caacgttcag
      721 tagatatgca gttggcagac ttgaatgatt catacgcaga tgctcaagct gcggttgata
      781 aagcatatac agccttaaca gaagcgacag taactagtac cgttgctggt acagttgttg
      841 aggtgaataa ggctgtttct aagaattcta catcgagcca aacagttgta cacattgtaa
      901 atcaaggtag tttgcaagta acaggtagtt tgacagaata cgatttggct aacattgcag
      961 tggatcaaga ggtcaagctg actagcaagg tatatcccga taagacttgg acagggaaaa
     1021 ttacctatat ttcaaactat ccatctagta atcaagctgg tgctgctaca acaggaacca
     1081 gtggttcagg tgccaaatat ccatttaagg cagctttaac tagtgaatta ggtgagttga
     1141 aacaaggatt ttctgtaaat attgaggttg tcaatacaag taaaaacatt ttaattccgg
     1201 tgtccgcagt tgtgccagaa gaagataaaa actatgtgtg gaccttggtt gatgggaaag
     1261 ctaagaaagt agaagttact ctggggaacg cagatgcttt gaaccaggaa gtgaccgctg
     1321 gtatagcagc aggagataag gttattacca ttccaactcc gaatctcgaa gatggcaaag
     1381 aggttgaagc caatgaagaa ccagcttatt agactaacaa gtatcaataa atcctacaaa
     1441 aatggtgatc aagaactccg agttttaaag aatattgatt tagaggtgga agagggagaa
     1501 tttcttgcca ttatggggcc ctctggttca gggaaatcaa ccttgatgaa cattattggg
     1561 cttttggatc gtttcagttc aggaaactat tggctggaag gtgaagaagt cagtcaactt
     1621 tctgagaaaa aattagctca ggttcggaat gaccaaatcg gtttcgtctt tcaacaattt
     1681 ttcctcttat cgaaacttaa cgcccttcaa aatgtagaat tacccttgat ttatgctgga
     1741 gttccatcaa gcaaacgtaa ggaactagca aaacagtatc ttgaaaaagt tgagttggca
     1801 gagcggatga cgcatttacc atcagaattg tctggtggtc aaaaacaacg ggtagctatt
     1861 gctcgtgcac tagtcaatac cccagctatt attttagcgg atgaacctac aggggctcta
     1921 gataccaaga caggtgagca gattatgcaa cttctgacgg agttgaataa tgaaggaaag
     1981 accattatca tggttacgca tgagcctgag attgctgcct acgcaaaacg aaaaatcgtt
     2041 ctacgtgatg gagtgatcac tgaagatagt cgaagggagg gagtttagat ggaaaattgg
     2101 aaattcgccc tcaaatctat tatggctcat aaaatgagat cgctccttac tatgttaggt
     2161 atcatcattg gtgtggctgc agttgtcatt attgtcgcca tgggaacagg ggtggccaaa
     2221 agcatagaaa agtctctagc tggtgatcaa aataatgttc aggtttactt tactgctttc
     2281 tccagttcag gtacagcttt cggtccgttt ggcacagcgt cgacagaaga accaatttta
     2341 gaggaagagc cggatttgac ggattctact ttaaaaggac tgctagagat tgatggcgta
     2401 tcaaactatt actccagcat cagcaacata gctgaagtca gatcgggcaa taaaaaagca
     2461 gagaatgtca ctattactgg tgttagccag aatttctttg ctgtgaaaga gtatgagatt
     2521 ttagctggtc gtcagtttac agctaacgac tatagtcgtt tttcacgtat gattatgtta
     2581 gatacggcat tagcagagaa gttatttggt tctatagaga actctctcaa tcagacgatt
     2641 agtgttaata gcaatgccta tctagtaata ggggtctaca aagatccgaa agcaggcaca
     2701 gctatttatg gaatgaattc aggtggaaat gccgtcatga ctaatacgca gctagctgct
     2761 gaaacaggca tgaaagaaaa ttctcaaatc tttgtccatg ttgaagatgt tactcgggca
     2821 acagaagttg ggaaggctgc tgcagattac ctgactaaag cgactggatt gaaagaagcg
     2881 agatacgaga tttatgatat gtcagctatg ttggaggagt ttaaatctca aatgtctggc
     2941 gttacattgt ttatcggtgc tgttgcaggt atctccttgc tagttggtgg tatcggtgtc
     3001 atgaatatca tgctggtatc agtaactgag cggactcgtg aaatcggtct tcgaaaagcg
     3061 cttggagcaa ctagaggaaa tatcttgatt caattcttga tagaagccat ggtcttgacc
     3121 actcttggtg gagcaattgg tctcgcaatc gctcagacaa tagttttctt gctcaatgtg
     3181 agcaaggcgt tgggcgaaag aatagctgct gaaatttcaa ttcctgttgt gttaggtagt
     3241 ctggctttct cagcagtagt tggtattgta tttggcgtac tacctgccaa caaagcatct
     3301 aagcttgatc caattgaagc tttgcgctat gaataatctg ttgaaacgat taggttttcc
     3361 tagtcgtttt attttttgag caaatcaatt gattttatca ctaattagag ataaaataaa
     3421 cgcatcaatt aagaaatagt gatttcaaag gagaacacat catggtagaa ttaggtattt
     3481 caacatttgg cgaaacaact ccactagaaa aaacagggga aacttacagc cacgacgagc
     3541 ggattcacca gctagtcaaa gaaatcgaac tagctgatgc ggtggggctt gatgtttatg
     3601 ggattgggga gcatcaccgc gaagattttg cggtttctgc acctgagatt gtgctggcag
     3661 ctggtgccgt caataccaag catatcaaac taacatcagc agtttcaatc ctttcctcca
     3721 tggaccctgt aaggctctat cagcagtaca ccaccattga tgctttgtca aatggcagag
     3781 cggaaatcat ggcgggtcgt ggttccttta cggagtcatt ccccttgttt ggctatgatc
     3841 tccacgacta tgaagaactc ttcgatgaaa agctagatat gcttctggaa atcgacaagg
     3901 aaaccaatct caagtgggat gggcatttta cccagtcagt cgataataag ccagtctatc
     3961 cacgtcctgt tcaggaagat tttccgatct gggttgcaac tgggggcaat gtagagtcca
     4021 ccatcaagat tgctaagaaa ggcctgccaa tcgtgtatgc agtcattggt gcaggtgccc
     4081 atcgctttaa acctttagtc gatgcttatc gcaaggttgc tcgcaactca ggtcatgatc
     4141 caaagaaaac gaaagttgca gcccattctt ggggttggat tgcagacgac catgacaagg
     4201 ctgtggaaga atactatcat ccaactaaag taattacgga caatattgcc aaagaccgcc
     4261 cacattggag cgagatgacc aaggcgcaat atctctactc tctgacagac gaaggagcaa
     4321 ccatcgtcgg agatccaaaa cgagtcgctg aaaaaattat caagactatt gaaacacttg
     4381 acttggaccg tttcttcctc catctcccaa tcggctcaat gccacatgaa gatgtcctcc
     4441 gtgctatcga actctacgga aaagaagttg cgccaattgt ccgagattat tttgcaaaaa
     4501 aagaagagaa tcagtcccaa gactgatgag ataaaaagga ggggacgtta taatagcctc
     4561 acttctctaa agtggagtca acatctcagc gcagtggttg attggaagat ttgttcgtgt
     4621 tttacactcc acatcttcct aatcaactgt gcgggggtgg gaagacgaac tctctatgat
     4681 tatttgagtt ctgtcccact cccaatccta aatatttttc ataatctcct gaaaatcctg
     4741 accatatggt tgggattttt tgaatgatta gatagtgacc ggtatgtaaa ttatttcata
     4801 gttgaaagtt aacttagatg atttgtttat tcttttaaag tgaatattta tataaatatt
     4861 ctataaatga tttgtgctat aatataaaaa aatcattatg gagattaata tggaaaatat
     4921 atttgctata aaaaataaga ttctagactt tccattttta gaagaagtaa atgaagatac
     4981 tacaataaac aaatgtggaa tttatatgct ctacatagac ttttttggtg ataatagtat
     5041 cctgccaatc tatatcggaa aatcaaaaga tgttcaaaaa cgattcgata gtcatttaaa
     5101 aaaaattatg tttctaaata gattatcaga atcaacttat ttatcttatt tttttgagag
     5161 agaagatcct ttttatgatg gaagtttttt ctactgtaaa atttttcagt atatgattga
     5221 ccatcaatgt actttaacag atttcaaaat gaaagtagtc gaattttgtt cgttag
//
LOCUS       DAZQCJ010000114         5227 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000114 DAZQCJ010000000
VERSION     DAZQCJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.115"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(90..1463)
                     /gene="dnaA"
                     /locus_tag="ACHU66_001895"
     CDS             complement(90..1463)
                     /gene="dnaA"
                     /locus_tag="ACHU66_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR3390155.1"
                     /translation="MNQEQLFWQRFIELAKVNFKPSIYDFYVADAKLLGINQQVANIF
                     LNRPFKKDFWEKNFEELMIAASFEIYGEPLAIQYQFTEDEQEIRNTTNTRSSIVHQVQ
                     TLEPATPQETFKPVHSGIKSQYTFANFVQGDNNHWAKAAALAVSDNLGELYNPLFIFG
                     GPGLGKTHILNAIGNKVLADNPQARIKYVSSETFINEFLEHLRLNDMESFKKTYRNLD
                     LLLIDDIQSLRNKATTQEEFFHTFNALHEKNKQIVLTSDRNPDHLDNLEERLVTRFKW
                     GLTSEITPPDFETRIAILRNKCENLPYNFTNETLSYLAGQFDSNVRDLEGALKDIHLI
                     ATMRQLSEISVEVAAEAIRSRKQTNPQNMVIPIEKIQTEVGNFYGVSLKELKGSKRVQ
                     HIVHARQVAMFLAREMTDNSLPKIGKEFGNRDHTTVMHAYNKIKTLLLDDENLEIEIT
                     SIKNKLR"
     gene            complement(1666..2430)
                     /locus_tag="ACHU66_001896"
     CDS             complement(1666..2430)
                     /locus_tag="ACHU66_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR3390156.1"
                     /translation="MEELRTLNISEIHPNPYQPRIHFDEKELLELAQSIKENGLIQPI
                     IVRKSSIIGYELLAGERRLRASQLAGLTTIPAVVKELTDDDLLYQAIIENLQRSNLNP
                     IEEAASYQKLISRGLTHDEVAQIMGKSRPYISNLLRLLNLSSQTKQAVEEGKISQGHA
                     RQLVSFSEEKQAEWVQLILSKDLSVRTLEKLIAANKKKHTKLKQRDQFLKEQEDSLSK
                     TLGTATKIIKKKNGSGEIRINFNDLDEFERIINNFK"
     gene            complement(2491..3687)
                     /locus_tag="ACHU66_001897"
     CDS             complement(2491..3687)
                     /locus_tag="ACHU66_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFR3390157.1"
                     /translation="MERIPYMKKYLKFAILFVIGFFGGLIGALSASFFQPRVQQANSA
                     ITSVSNVQYNNETSTTKAVEKVQNAVVSVINYQKSANNSLGAIFGNIESSDELAVAGE
                     GSGVIYKKDGQYAYIVTNTHVINNAEKIDILLASGEKISGELVGSDTYSDIAVIKISA
                     DKVTAVAEFADSDTIKVGETAIAIGSPLGSVYANTVTQGIISSLSRTVTSQSKDGQTI
                     STNAIQTDTAINPGNSGGPLINTQGQVIGITSSKITSSSANSSGVAVEGLGFAIPAND
                     AVAIINQLEKTGQVSRPALGVHMVNLTTLSTSQLEKAGLSNTELTSGVVIVSTQSGLP
                     ADGKLETFDVITEIDGEAIQNKSDLQSALYKHQIGDTITVTYYRNNQKQTVDIKLTHS
                     TEELSE"
     gene            3861..4340
                     /gene="rlmH"
                     /locus_tag="ACHU66_001898"
     CDS             3861..4340
                     /gene="rlmH"
                     /locus_tag="ACHU66_001898"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR3390158.1"
                     /translation="MKIKLITVGKLKEKYLKEGIAEYSKRLGRFTKLDMIELPDEKTP
                     DKASQAENEQILKKEADRIMSKIGERDFVIALAIEGKQFPSEEFSQKLSDIAVNGYSD
                     ITFIIGGSLGLDSCIKKRANLLMSFGQLTLPHQLMKLVLIEQIYRAFMIQQGSPYHK"
     gene            4365..5048
                     /locus_tag="ACHU66_001899"
     CDS             4365..5048
                     /locus_tag="ACHU66_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFR3390159.1"
                     /translation="MNQKEYRVFEGLRIACSLTFISGYLNAFTFVTQGGRFAGVQSGN
                     VISLAYFLAKGDLAQVVNFSIPILFFVFGQFFTYLARRYFEKQTWSWHFGSSVMMLVL
                     ILLTIILSPIMPASFTIASLAFVASIQVETFRRLRGAPYANVMMTGNVKNAAYLWFKG
                     VIEKDSELRKTGRNILLTIIGFMLGVIISTHLSFQFEEYALIGLILPVLYINYELWQE
                     KRPTRGRSK"
     gene            complement(5046..5119)
                     /locus_tag="ACHU66_001900"
     tRNA            complement(5046..5119)
                     /locus_tag="ACHU66_001900"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5083..5085),aa:Arg,seq:tct)
BASE COUNT         1573 a          906 c          949 g         1799 t
ORIGIN      
        1 aaagtgatga aagaccaata aaactgcgga aattaacttg ttcacaactt gtggaaaaaa
       61 cagagttttt taaaaaagtt atacacaggt taacgaagtt tattttttat actggtaatc
      121 tctatttcta aattctcatc atccaagagg agagttttta ttttattgta tgcatgcata
      181 acggttgtat ggtctcgatt accaaattct ttcccaattt ttggaaggga attgtctgtc
      241 atttcacgtg ctaaaaacat agcaacttgt cgcgcgtgaa cgatatgttg aacacgctta
      301 gaacctttta attctttcaa gctgacaccg tagaaatttc ccacttcggt ttggattttc
      361 tcaataggaa taaccatatt ttgtggattt gtttgttttc ttgatcgaat agcctcagca
      421 gcaacctcga cacttatctc agacagttga cgcatagtgg ctatcaaatg gatatctttt
      481 aaggcacctt caaggtcacg tacgttcgaa tcaaattgcc cagctagata ggatagcgtc
      541 tcatttgtaa agttgtaagg caggttctcg cacttgttac gtaaaattgc gatacgtgtt
      601 tcaaaatcag gtggagtgat ttcactggtt aacccccatt tgaaacgtgt tactagtctt
      661 tcttccaaat tgtctaagtg atcgggatta cggtcgcttg tgagtacaat ctgcttattt
      721 ttttcatgaa gcgcattaaa agtatggaaa aattcttcct gtgttgttgc tttattacgg
      781 agagactgaa tgtcatcaat tagaagtaag tccagattgc gataggtttt tttgaaactt
      841 tccatatcat tgagacggag gtgttctaaa aattcattga tgaatgtttc cgatgagaca
      901 tattttatcc ttgcctgggg attatcggct agaaccttat ttccaatcgc atttaaaata
      961 tgagtttttc caagaccagg accaccaaaa atgaataatg gattgtagag ctcacctagg
     1021 ttatcagata cagctaaagc tgcagccttt gcccagtgat tattgtctcc ttgtacaaaa
     1081 ttagcaaagg tgtactggga ttttatacca gaatgaaccg gtttaaaagt ttcttgagga
     1141 gtagccggct caagtgtctg tacctggtga actattgaac ttcttgtgtt tgtagtattc
     1201 ctaatctcct gttcatcctc tgtaaattga tattggatgg caagaggctc tccgtagatt
     1261 tcaaaactag cggcaatcat taactcttcg aagttttttt cccagaaatc ttttttaaat
     1321 ggacgattta agaaaatatt ggcaacttgc tggttgattc cgagtaattt tgcatcagcg
     1381 acataaaaat cataaataga tggcttaaaa tttacctttg ccaattcaat aaatcgttgc
     1441 caaaaaagtt gttcttggtt catattttcc ccctttcaca ttatattcgt tagaatagtg
     1501 taccataaaa aacaaaagtt ttccacagat aattttaaaa atccacactg ttaagaaaag
     1561 ttttccacaa gatgtgaata aaagtcagaa cctttgctgg tcctgacttt ttgtaatgat
     1621 tttagtgtgg aaaagagtgt ttataaaaat aaattgtaaa caagtctatt taaaattgtt
     1681 gataattctt tcgaattcat cgaggtcatt aaagttaatc cgaatttctc cgctcccgtt
     1741 tttcttcttg ataatttttg tagctgttcc aagagtttta ctgagtgaat cttcctgttc
     1801 ttttaaaaat tggtcgcgtt gtttaagctt agtgtgtttt ttcttatttg cagctattaa
     1861 tttttcaagc gtacgcacac ttaaatcctt tgataaaatg agttgaaccc attcggcttg
     1921 cttttcttct gaaaatgaca ccaattgtcg cgcgtgccct tgtgaaattt ttccttcttc
     1981 tacagcttgt ttagtctgag atgatagatt tagtaggcgc aataaattac tgatatatgg
     2041 tcttgatttt cccatgattt gagcaacttc atcatgtgtt aaccctctac taatcaattt
     2101 ttgataagag gctgcttctt ctatcggatt taagttagaa cgctgcagat tctctatgat
     2161 agcctgatag agtaaatcat catcagttag ttcctttacc actgctggta ttgtagtcag
     2221 tccagctaat tgactggctc gcaaccttct ttctccagct aataattcgt atccgataat
     2281 agaagatttt cttacaataa tcggttgaat taagccattt tccttaatag attgagcgag
     2341 ctcaagtagc tccttttcat caaaatgaat tcttggctga tagggattgg gatggatttc
     2401 tgaaatattt agtgtacgta attcttccat atatccttat tctagcaaaa aaaatgagac
     2461 tttacaattg tgtaaagtct catttgtcaa ttattcgcta agttcttctg tagaatgtgt
     2521 caacttaatg tcaacagttt gtttctgatt attgcggtaa taagttacag tgattgtatc
     2581 tccaatttga tgtttgtaga gagcgctctg gaggtcactc ttattttgaa tagcttctcc
     2641 gtcaatctca gtaataacat caaaagtttc taattttcca tctgcaggta gcccactttg
     2701 tgtagagaca attactacac cggatgttaa ttccgtattt gataatccag ctttttctaa
     2761 ttgactagtt gacaaggtcg tcaagttaac catatgaact ccaagagcag gtcggctaac
     2821 ttgtccagtt ttttcaagct gattgataat agctacggca tcatttgcag gaatagcaaa
     2881 tcccaaccct tctacagcca cgcctgagct atttgcagaa cttgaggtaa ttttgctaga
     2941 ggtaatgcct atcacttgtc cttgggtatt gattaacggt ccgccagagt ttccagggtt
     3001 gatagctgta tcagtttgaa tagcgttagt tgagattgtt tgtccatctt ttgattgtga
     3061 agtaactgtc cgacttaagc tagaaataat accctgggta actgtattgg cgtagacgct
     3121 acctagagga ctaccaattg cgatagcagt ttctccaact ttaattgtat cggaatcagc
     3181 aaattcagca acagcagtga ctttatctgc tgatattttt ataacagcta tatcagaata
     3241 tgtatcagaa ccaacaagtt caccgctaat tttttctcca gatgctaaaa ggatatcaat
     3301 cttttctgcg ttattaataa catgcgtatt tgtcacaata taggcatatt gaccatcttt
     3361 tttgtagata acaccagatc cctccccagc aacggctagt tcgtcagatg attcaatatt
     3421 tccaaagata gcaccaagac tattgttggc tgacttttgg taattaataa cagacacaac
     3481 agcattttgt actttctcta cagcctttgt agtagaagtt tcattattat attgaacatt
     3541 gctgacacta gtgatggcag aatttgcttg ttgaacccgt ggctggaaga aagaggctga
     3601 taaggccccg ataagacccc caaaaaatcc aattacaaat aaaatcgcaa atttcaaata
     3661 ttttttcata taaggaatcc tttccatttc tttaattttc tttaagagta ctatttcttt
     3721 cttaattata acttaaagtt tttaaaaatc aatccacaac ctgtggataa ctctgtgaat
     3781 tatgtgaaaa actgtagcca tatcctaatt ttctaaaaat attttctgtc gtatgcttgt
     3841 ggatatgtta caattatttt atgaaaataa aattgattac cgttggaaaa ttgaaagaaa
     3901 agtacctcaa agaaggtatt gcagaatata gtaaacgatt gggacgattt actaagttgg
     3961 atatgattga gcttcctgat gaaaaaacac cagataaagc cagtcaggca gagaatgaac
     4021 aaatattaaa aaaagaagcc gatagaatta tgtctaaaat tggagagcga gattttgtca
     4081 ttgccttagc gatagaaggg aaacaatttc catcggaaga atttagtcaa aagctatctg
     4141 atattgcagt aaatgggtat tcagatataa cttttatcat cggtggtagt ttgggtctcg
     4201 attcttgtat taaaaaaaga gctaatttgt tgatgagttt tggacagttg acacttccac
     4261 atcaattaat gaaattagtt ctcatcgagc agatttatcg tgcatttatg attcagcagg
     4321 gaagcccata tcataagtag tttcacgtga aacaaggagt gaaaatgaat caaaaagagt
     4381 atcgtgtttt tgagggattg agaattgctt gttcattaac gtttatcagt ggttatttaa
     4441 atgcctttac ttttgtgact cagggtggtc gctttgctgg cgtacaatct ggaaatgtta
     4501 tttccctagc ttatttttta gctaaaggtg accttgcgca ggtagttaat ttttccattc
     4561 ccattttatt ttttgtattc ggacaatttt ttacctactt agcaagaagg tattttgaaa
     4621 aacaaacatg gtcttggcac tttggtagta gtgtaatgat gttagttctt attttactaa
     4681 ctatcattct ctcacctata atgcctgcgt cttttacaat tgctagtcta gccttcgtag
     4741 cctctattca agtagaaaca tttagaaggt tacgaggtgc tccgtatgcc aatgtgatga
     4801 tgacagggaa tgtcaaaaat gctgcttatc tctggtttaa aggagttatt gaaaaagatt
     4861 cagaacttag aaaaacaggt agaaatatct tattaactat tatagggttt atgctaggtg
     4921 tcatcatatc tactcaccta tccttccaat ttgaagaata tgcccttatc ggtctgattt
     4981 tgccagtttt atatattaat tatgaattat ggcaagaaaa aagacctact cgaggtaggt
     5041 ctaaatgatt ccagcaggat tcgaacctgc gaccgttcgc ttagaaggcg aatgctctat
     5101 ccagctgagc tatggaacct caactatttt attttacatt aaacaaagca ctctgtcaat
     5161 acaataaaaa atcaaaaaat gtattgacaa acaaagtcac atgtattata ctagttattg
     5221 ttctaaa
//
LOCUS       DAZQCJ010000115         4984 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000115 DAZQCJ010000000
VERSION     DAZQCJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.117"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            64..744
                     /locus_tag="ACHU66_001901"
     CDS             64..744
                     /locus_tag="ACHU66_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFR3390160.1"
                     /translation="MAKEKARYFTFLLYPESIPSDWELKLELLGVPIAVSPLHDRDKS
                     DVEGQQYKKPHYHVIYVSKNPVTADSVRMKIKRSLGDNSVALVQIIRTSIENTYLYLT
                     HESKDAIEKKKHVYDKADITLLSNFDIDRYITLDVEEKDDMLNEVCDLIDEYDIANMR
                     ELRRFIKLHGAEHGLPSIKIINSVLRSHTGLIRLYFDAVYQERKYGSNIPNVNQETGE
                     ILSEDSDD"
     gene            737..1144
                     /locus_tag="ACHU66_001902"
     CDS             737..1144
                     /locus_tag="ACHU66_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390161.1"
                     /translation="MTDRNELINDIAELKAKRDRLLAQMKEAEQWESVAWDSYYAVAD
                     HVKALEKKQEIGRNYWESSQRAISHQFDFVADQANKVKKVLAKKRYDLLDEEIDKLMN
                     EVRELADVLGIEIDELPLDFPFFALSAEGVSDE"
     gene            1137..1745
                     /locus_tag="ACHU66_001903"
     CDS             1137..1745
                     /locus_tag="ACHU66_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390162.1"
                     /translation="MSKTVSELSAELGVSKQYLNRVLSQNNLGRKIGNKKLVSDSDVK
                     SLLSILKNNIGNQKSETENIVSDIPSKSTGKRDTGENGNQKSETKSETSFRYLHEQLE
                     IKGKEVERLHQLLDQSQQLLLNEQKKNQLLLEEKSEEYSDKGFHQNLIESMQGSLSWN
                     RQEVSRLNEKLENSVSEKWFYVVVSVAVFLFVMLLVLVWGNF"
     gene            2073..3572
                     /gene="mobV"
                     /locus_tag="ACHU66_001904"
     CDS             2073..3572
                     /gene="mobV"
                     /locus_tag="ACHU66_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFR3390163.1"
                     /translation="MSYMVARMQKMKAGNLGGAYKHNERVFETHSNKDIDPSRSHLNY
                     ELTDRDRSVSYERQIKDYVNENKISNRAIRKDAVLCDEWIITSDKSFFEKLSEEETRE
                     FFETAKNYFAENYGEDNIAYASVHLDESTPHMHMGVVPFQDGKLSSKAMFDKEELKKI
                     QEDLPKYMNEHGFELERGELNSEAKHKTVAEFKQEMAGKEIEKQLVLEYGAPEYVNHQ
                     EEFVTKEEYQEAYEVFQNELGAWLDKSDFSWRETTFQEKIDWVKNHQQEELQQLVSSR
                     KPLEDEIRTLNEVLKEKYEELDKIELRASESLSELSGVEGYINNLEKHSSALESKIGG
                     LENEYLRLAKQNARLSDLKIMSEKELAEIQPKKGMFGKEHVELTKEQFEKFKGLIYRS
                     KNLVHEKELENDQLRRQMPLRSSKNGFEASLQRAKDKTKGESIDRLKSEIRGLKNENS
                     VLRQQNDKMLGKLREFMPDKALNNFISELKSIKPIVKIVKRVIEKGLGL"
     gene            complement(3833..4285)
                     /locus_tag="ACHU66_001905"
     CDS             complement(3833..4285)
                     /locus_tag="ACHU66_001905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390164.1"
                     /translation="MKSFKKSIIVFVTLFSMLGFISISTVFADNTVTTSNTNISTTTD
                     YSYTGEIVPYKTSYGNGGYTKIELYGGGREVWWSVKPNTSKSYTYSGLITVTFSDNTT
                     RIYPVSKSGKGGQSVSGSFGLPKKARNAGISGIAYGAGGVQISIPTIE"
     gene            4357..4626
                     /locus_tag="ACHU66_001906"
     CDS             4357..4626
                     /locus_tag="ACHU66_001906"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390165.1"
                     /translation="MKKNFIAIIKNILKNKNIPKYCFEIIEKDGSTCFLSTNYFDVIN
                     FEILNIFGYIGVKFEIITAYENGKLVETQIIVDDIELIKVEKFFD"
     gene            4849..>4984
                     /locus_tag="ACHU66_001907"
     CDS             4849..>4984
                     /locus_tag="ACHU66_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFR3390166.1"
                     /translation="MKKRLTITLSESVLENLEKMAKEMGLSKSALISVALENYKKGQV
                     K"
BASE COUNT         1755 a          747 c         1095 g         1387 t
ORIGIN      
        1 aaaaaaaagc cgagctggca ggcgggcttt taaaagtatt ataacttggg ttaattataa
       61 cttatggcta aagaaaaagc aagatacttc acttttttac tctatcctga gtcaatccct
      121 agtgattggg aattgaaatt agaattgctg ggcgtaccta ttgcagtcag ccctttgcat
      181 gatagagaca agagtgatgt tgagggacaa caatataaaa aaccgcacta tcatgtgatt
      241 tatgtttcaa aaaatccagt cacggcagat agtgtgagaa tgaaaattaa gaggtcactt
      301 ggagataata gcgttgctct agttcagata attagaacta gcattgagaa cacgtattta
      361 tatcttacgc atgaatcaaa ggacgctatc gaaaagaaaa agcacgtcta tgataaggct
      421 gacataactt tgcttagtaa ctttgatata gaccgttata taacgcttga cgtcgaagag
      481 aaagacgata tgctcaatga ggtttgtgac cttattgatg agtatgatat tgcaaatatg
      541 cgtgaattga gacgttttat taaacttcat ggggctgaac atggtttacc tagtatcaaa
      601 attataaatt cagttttacg ttcgcatact gggttaatac gtctgtattt tgatgctgtc
      661 tatcaagaac gtaagtatgg cagtaatatc cctaatgtta atcaagagac aggcgagatt
      721 ttaagtgagg atagcgatga ctgatagaaa tgagctgatt aacgatattg cagagttgaa
      781 agcaaaaaga gacagactac tggctcaaat gaaagaggcg gaacaatggg aaagcgtagc
      841 gtgggatagt tactatgcag tcgcagacca tgtgaaagct cttgagaaaa agcaggagat
      901 aggtcgaaac tattgggaaa gttctcaaag agctattagt catcaatttg actttgtggc
      961 tgaccaagct aacaaagtca aaaaggtgtt ggctaaaaag agatatgatt tgctagatga
     1021 ggaaatcgac aagctgatga acgaagttcg agagctggca gatgtccttg ggattgagat
     1081 tgacgagctc cctcttgatt ttcctttctt cgctctaagt gcagaggggg tttctgatga
     1141 gtaaaacagt atcagaatta tccgcagaat taggggtaag caaacaatat ttaaaccgtg
     1201 ttttaagtca aaataatctc ggtcgaaaaa tcggaaacaa aaagttagtt tccgatagtg
     1261 acgtaaagtc actattatca atacttaaga acaatatcgg aaaccaaaaa tcggaaactg
     1321 aaaatatagt ttccgatatt ccgtcaaagt caactggtaa aagggataca ggcgaaaacg
     1381 gaaaccaaaa atcggaaact aaatcggaaa ctagtttccg atatctccat gaacagttgg
     1441 aaataaaggg gaaagaggtt gaacgacttc atcagttgct tgaccaatct cagcagttgc
     1501 ttttgaacga gcagaagaaa aaccagctct tgctggaaga aaaatccgaa gagtattcgg
     1561 ataaagggtt tcatcaaaat cttattgaat ccatgcaagg cagtttgtct tggaataggc
     1621 aagaagtaag tcgcttgaac gaaaagttgg aaaactcggt tagtgagaaa tggttttatg
     1681 tagttgtttc agtagccgtt tttttatttg tgatgttact tgtcttggta tggggtaatt
     1741 tttaaagagc ataagcaagc tgaccttttg gggtcagctt tttttgtcag acaatagcga
     1801 agcggtctga cttgattgtc caaataggac aatctgcacc ctttttggag ggtgcttttt
     1861 tcgtgcgttg actcgcacgt tagctgtctg caagaccctt cggagaacgc acatttacga
     1921 agtaaagtat aatacgttat actttacatg gaagttagac cgaaaatatg ctaaactact
     1981 agataagata ttgcgtattt agacgagcaa tagacttgtc ggctcgtctg tcagtcgcta
     2041 ttcgactgac gctgataagg aggggtaaga acatgagcta catggttgcg agaatgcaga
     2101 agatgaaagc tggcaatttg ggaggagctt ataagcataa tgagcgtgtt tttgagacac
     2161 actcaaataa agatatagac ccgagcaggt cgcacctcaa ctatgagttg acggatcgtg
     2221 accgctcggt gtcgtatgaa cggcaaatta aagattatgt gaacgaaaat aaaatatcta
     2281 atcgagctat tcgaaaagat gcggttttgt gtgatgaatg gattatcaca tcggataagt
     2341 cattctttga aaaattaagc gaggaggaaa caagagaatt ttttgagacc gctaaaaatt
     2401 attttgcgga aaattatggt gaggacaata tcgcttatgc aagcgtacac ttagacgaaa
     2461 gcacccctca catgcatatg ggagtggtgc ctttccaaga tgggaaattg tcttctaagg
     2521 caatgtttga caaagaagaa ctcaaaaaaa ttcaagaaga tttgccaaaa tacatgaatg
     2581 agcatggttt tgagctagag cgtggcgagc tgaatagcga agccaagcat aagacggttg
     2641 ctgaatttaa gcaagaaatg gctggtaagg aaatcgagaa acagttagtt cttgaatatg
     2701 gggcgcctga gtatgttaat catcaagaag agtttgtgac caaagaagag tatcaagaag
     2761 cctatgaggt ttttcaaaat gagttaggag cttggcttga taagtctgat ttttcttggc
     2821 gagaaacgac ttttcaagaa aaaatagatt gggtcaaaaa tcatcagcag gaagaactac
     2881 aacaattagt aagctctaga aagcccctag aagacgaaat aaggactttg aatgaagttc
     2941 ttaaggaaaa atacgaggag ttggacaaaa tcgagctacg agcctctgag agcctctcag
     3001 agctatctgg tgtcgaaggg tatataaata acctagaaaa acattcaagc gccttagaaa
     3061 gcaaaatagg gggcttagaa aacgaatatt taagactggc taaacaaaat gcccgattaa
     3121 gcgacttgaa aatcatgtct gaaaaagaat tggctgaaat tcagccgaaa aaaggcatgt
     3181 ttggaaaaga acatgtcgaa ttgactaaag agcaatttga gaaatttaag ggattgatat
     3241 accgaagcaa gaatttagtt catgaaaagg aattagagaa tgaccaacta aggcggcaaa
     3301 tgcctctgag aagctcaaaa aacggttttg aggcgagttt gcaacgggct aaggataaaa
     3361 ctaaaggaga aagcatagac cgccttaaaa gcgaaattag agggcttaaa aatgaaaact
     3421 cggttttgag acagcaaaat gacaaaatgc taggtaagtt aagagagttt atgcctgata
     3481 aagcgttaaa taattttatt tctgaactta aatcaataaa acctatcgtg aagatagtta
     3541 aaagagttat tgaaaaaggc ttagggctct aagcgattta tgccgagaaa actcttgcta
     3601 ggaagctatg cgaaatagac tagtaggggt cgagtcgaca ggctgaaagc ttgccgaccg
     3661 aacacgacag tcagatttca gcataggttt cttcctagca agaggaaatt ggaataaaga
     3721 aagcgtatca aagtaaaact aaaaggacta agtcctgtgt aatacaggcc ttagtccttt
     3781 acttaaaata gaatgtccaa ctttttgggg tcagtatata ttttagtctt ttttattcta
     3841 tagttgggat agagatttgt actcctcctg cgccatatgc aattccgctt atacctgcat
     3901 ttcttgcttt tttaggtaaa ccgaatgaac cactaactga ttgacctcct ttgccagatt
     3961 tgctgacagg atatattcta gtagtgttat ctgagaaagt aactgttatc aatccagaat
     4021 atgtataact ttttgatgta ttaggtttaa ctgaccacca aacttctcga ccaccaccat
     4081 ataattcgat ttttgtatat ccgccatttc cgtaacttgt tttgtaagga actatttcac
     4141 cggtataaga ataatcagtt gtagttgaaa tatttgtatt tgaggtagtg actgtattat
     4201 ctgcaaaaac agtagaaatg gatataaatc ctaacataga aaaaagagtg acaaatacaa
     4261 ttattgattt tttgaaactt ttcatgaaag tttacctccg tttttttgtt ataattatag
     4321 caaaaaaata attaaaaaga aagcgttatc atagaaatga aaaagaattt tattgcaatt
     4381 ataaaaaaca tattaaaaaa caaaaatatc cctaaatatt gttttgaaat tattgaaaaa
     4441 gatggaagta cttgttttct ttcgacaaat tactttgatg taattaattt tgaaatatta
     4501 aatatttttg gatatatagg tgttaagttt gaaattatta ccgcttatga aaatgggaaa
     4561 ttagttgaaa cgcaaattat tgtggatgac atagagttga taaaagttga aaaatttttt
     4621 gattaaagag aagaagaggg ggcgtactac gacccccctt ttaagtgccg agtgccaaat
     4681 tgctaaaaaa agtgccgtaa agctagatac atcaagggtt tagctcatgc tacacttcgg
     4741 cgacattttg gcgacatttt cggcgacaaa aagaaaaaaa tacgataaat gttgcaatgt
     4801 atgcacagtt atgctactat ggttttataa atttttgaga ggtggaacat gaaaaaaaga
     4861 ttgacgataa cactcagtga atcggtgctt gaaaatcttg aaaaaatggc aaaagagatg
     4921 gggttgtcaa aatctgcact gatttcagtt gccttggaaa actacaagaa aggtcaagta
     4981 aaat
//
LOCUS       DAZQCJ010000116         4965 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000116 DAZQCJ010000000
VERSION     DAZQCJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.118"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            70..1635
                     /locus_tag="ACHU66_001908"
     CDS             70..1635
                     /locus_tag="ACHU66_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFR3390167.1"
                     /translation="MKRKLSIFLLTLIAVFSVTTGVRADEYLRVGMEAAYAPFNWTQE
                     DDSNGAVPIEGTNQFANGYDVQVAKKIAASMDKELLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTEERKKEIAFSDSYYTSIPTLVVRSDSQYANATSLADFAGAKITAQQGVYLYD
                     LIDQIEGANKQTAMGDFSQIRQALESGIIDAYVSERPEGRTAEAANKAFKMVELADSF
                     ETNAEDVTIAVGMRKDDERIAQVNKVLATISEEEQIALMDDMIENQPVEEASEGETPS
                     FFAQVWNIVVNNWQQLLRGTGMTLLISILGTIIGTAIGLLIGVFRTAPKAANKALAIG
                     QKVLGWIINIYIEIFRGTPMIVQSMVIYYGTAQAFGLNLDRTLAAIFIVSINTGAYMS
                     EIVRGGIFAVDKGQFEAATALGFTHNQTMRKIVLPQVIRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTVIAIIYFILTFTVTRILRAVEKHFDTDTYT
                     TGANQMQVEVPHD"
     gene            1628..2371
                     /locus_tag="ACHU66_001909"
     CDS             1628..2371
                     /locus_tag="ACHU66_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002886432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR3390168.1"
                     /translation="MTKNVILEIKNLKKSYGQNQVLKDISISVEKGEVISIIGSSGSG
                     KSTFLRSINLLETPTEGQILYHGQDVLDKGYDLTTYREKLGMVFQSFNLFNNLNVLEN
                     AIVAQTTVLKRDRAEAEKIAKENLNKVGMTEQYWQAKPSQLSGGQKQRVAIARALSVD
                     PEVILFDEPTSALDPEMVGEVLKTMQDLAKSGLTMLIVTHEMEFARDVSDRVIFMDKG
                     VIAEEGNPQQIFENPQEERTREFLQRFLG"
     gene            complement(2495..2704)
                     /locus_tag="ACHU66_001910"
     CDS             complement(2495..2704)
                     /locus_tag="ACHU66_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390169.1"
                     /translation="MTSYEKVAAVVGWVREKKITGYRISKETNAREMSVIALAQGRAK
                     IDNISFATALGLIDFYDKNHKKFEN"
     gene            complement(2790..3692)
                     /locus_tag="ACHU66_001911"
     CDS             complement(2790..3692)
                     /locus_tag="ACHU66_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR3390170.1"
                     /translation="MVLGPIVFIGSFLFFVLIALILIASGLYVVKQQTVAIIERFGKY
                     QKTSTSGINFKIPFGVDVIAARIQLRMLQSEIVVETKTQDNVFVTMNVATQYRVNENN
                     VTDAYYKLMHPEAQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQKQVAEEMSTYG
                     YVIVKTLITKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRL
                     HGVGIAQQRKAIVDGLADSIRELKESNVSLSEEQIMSILLTNQYLDTLNNFAQGGNQT
                     IFLPGNPEGVEDIRTQILSSLRAK"
     gene            complement(3702..4952)
                     /gene="ilvA"
                     /locus_tag="ACHU66_001912"
     CDS             complement(3702..4952)
                     /gene="ilvA"
                     /locus_tag="ACHU66_001912"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019322143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HFR3390171.1"
                     /translation="MITAKNVEQAYSVLKRVVVRTPLDYSRYLSEKYGATIYLKRENE
                     QRVRSFKIRGAYYAISQLTDDEKSRGVVCASAGNHAQGVAYTCQEMQIPATIFMPITT
                     PQQKIGQVKFFGGDYVEIRLVGDTFDESAKAAQDYTQESGKTFIDPFDDENVQAGQGT
                     VAYEILEEAEEQTISFDQILVPIGGGGLVAGVSAYLKEHAPEIKIVGVEASGARSMKA
                     AFDKGRPVKLDQIDKFADGIAVQKVGKSTYEVARKYVDRLIGVDEGWISGTILDLYSK
                     LGIVAEPAGAATIAALEVVKDKIKGQTICCIISGGNNDINRMPEMEERALIYEGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRANKGTGPVLIGIALGDKTDYTQFI
                     SRLEEFDPQYINLHENDSLYKMLV"
BASE COUNT         1440 a         1134 c          944 g         1447 t
ORIGIN      
        1 agtacaagga tattgaaatt tgtcagaatt tttaataaaa tggtgtaata gcattttaaa
       61 ggagacatta tgaaacgtaa actcagtata tttctgctga ccctaatcgc tgttttttct
      121 gtaacaacag gggttcgggc agacgaatac ctgcgggtcg gtatggaagc tgcctatgca
      181 ccatttaact ggacccaaga agatgatagc aacggtgccg ttcctattga gggaaccaat
      241 caatttgcca acggatatga tgttcaggtt gcaaaaaaaa tcgctgcatc catggacaaa
      301 gaactcttgg ttgttaaaac caagtgggaa ggcttagtcc ctgctcttac atctggtaaa
      361 atcgatatga ttatcgccgg tatgagcccg acagaagagc gcaagaaaga aatcgccttc
      421 tcagatagct actacacttc aattcctacc ctggttgttc gttctgatag tcagtacgca
      481 aacgcaacca gcttggcaga ttttgctggc gcaaaaatca ctgcccagca aggggtttac
      541 ctctatgatt tgattgacca aattgaaggg gctaacaaac agactgccat gggagatttc
      601 tcacaaatcc gtcaagcttt agaatctggc attatagacg cctatgtttc tgaacgtcca
      661 gaaggtcgta cagcagaagc tgcaaataaa gctttcaaaa tggttgaatt ggcagacagt
      721 tttgaaacca atgccgaaga cgtgaccatt gctgtcggta tgcgcaaaga tgacgagcgt
      781 atcgctcagg tcaacaaagt cttagcaact atttcagaag aagagcaaat cgcacttatg
      841 gatgatatga ttgaaaacca gcccgttgaa gaagccagcg agggcgaaac acctagtttc
      901 ttcgcccaag tttggaatat tgttgttaac aactggcaac aacttctgcg cggaactggg
      961 atgacattac tcatttccat cctcggtaca attatcggta ctgctatcgg tctcctcatc
     1021 ggtgtcttcc gtacagctcc aaaagcagca aacaaggcgc ttgctattgg tcaaaaagtg
     1081 ctcggttgga ttatcaatat ctacatcgaa attttccgtg gtacgccgat gattgtacaa
     1141 tccatggtta tttactatgg taccgcccaa gcattcgggc tcaatcttga ccgcacactt
     1201 gccgctatct tcattgtatc catcaacaca ggtgcctaca tgagtgagat tgtccgcggt
     1261 ggtatctttg ccgtcgataa aggccaattt gaagctgcta ctgctctcgg atttactcat
     1321 aatcagacca tgcgtaagat tgtccttcca caggttattc gtaacatctt accagcgact
     1381 ggtaatgaat ttgtcatcaa tatcaaagat acttctgtat tgaacgttat ctcagttgtt
     1441 gaactctatt tctcaggaaa tactgtcgca acccaaacct atcaatattt ccaaaccttt
     1501 acggttattg ctattatcta cttcatccta acctttactg tgacacgtat cttacgcgca
     1561 gttgaaaagc attttgatac agatacatac acaacaggtg caaaccaaat gcaagtcgag
     1621 gtgccacatg actaaaaatg taattcttga aatcaaaaat ctaaagaaat catacggaca
     1681 aaatcaagtc ctcaaagaca tctctattag cgttgaaaag ggcgaggtta tctcaatcat
     1741 cggttcatct ggttcgggaa aatcaacctt cctccgatct atcaaccttt tggaaacacc
     1801 aacagaagga cagattctat accacggaca agatgttttg gataaaggat atgacttgac
     1861 gacttaccgt gaaaaactag gtatggtatt ccaatccttc aaccttttca acaatctaaa
     1921 tgtattggaa aatgccattg tagcacaaac tacggtactg aaacgagatc gagctgaggc
     1981 agagaaaatc gccaaggaaa acctcaacaa ggttggcatg accgaacagt actggcaagc
     2041 caaaccaagt caactctctg gtggacagaa acaacgcgtg gccattgcac gtgccctatc
     2101 tgttgaccca gaagttatcc tctttgatga gccgacttct gctctcgatc cagagatggt
     2161 cggggaagtt ctcaaaacca tgcaagattt ggcaaaatca ggattgacga tgcttatcgt
     2221 aactcacgaa atggaatttg cccgtgacgt atctgaccgc gttatcttta tggataaggg
     2281 tgtcatcgcc gaagaaggca accctcaaca aatctttgaa aatccacaag aagaacgcac
     2341 acgtgaattc cttcagcgtt ttcttggtta aactcaatct gttccataga agaccattac
     2401 ttaccagttt caaaactggt aagttttttt gactaaaaaa aagaggaatc gcaaaattcc
     2461 tctttttgga catctctaaa tgaaaccatt tagattagtt ttcaaatttc ttgtggtttt
     2521 tgtcgtaaaa atcaatcaaa cccaaggcag ttgcaaatga aatattgtca atcttagcgc
     2581 gaccttgtgc aagagcgata actgacattt cacgagcatt tgtttcctta ctgatacggt
     2641 aacctgtgat tttcttttca cgaacccagc cgacaacagc ggctactttt tcgtaactag
     2701 tcattagaaa cccctttctt ttttattcgt gcaatttagt ataatatgtt ttaacctatt
     2761 ttgtcaagta aaatgttcgt tttttaccat cattttgccc taagagatga tagtatttgc
     2821 gtccttatgt cttcgacccc ctccggattc cccggaagga agatagtttg attacctcct
     2881 tgtgcaaaat tattgagagt atccaagtat tggttggtca gcaagataga cataatctgc
     2941 tcttcagata ggctaacatt tgattctttc agttcacgaa tcgaatctgc cagtccgtct
     3001 acaatcgcct tacgttgttg tgcaatacct acaccatgca agcgatcctt ctcagcctct
     3061 gcttctgctg ctgtgacaat tttaatcttg tcagcttctg ccaattcttg ggccgcaacc
     3121 cgtttccgct gagctgcgtt aatttcattc atagattgct taacctcggc gtctggctct
     3181 accttggtaa ttaaggtttt gacaataaca taaccgtagg ttgacatctc ttctgctact
     3241 tgtttttgta cctcaagtgc aatctcatcc tttttctcga agagctcatc cagggtcaat
     3301 ttaggcacag atgaacgcag agcatcttca atataggact taatctgtgc ttctgggtgc
     3361 atgagcttgt aataggcatc cgtcacatta ttttcattta ctcgatattg agttgccaca
     3421 ttcattgtga caaaaacgtt atcctgcgtc ttggtttcta ccacaatttc actttgcaac
     3481 atccgcaatt gaatgcgagc tgcaatgaca tcaactccaa atggtatttt aaagttaata
     3541 cctgaagtag atgttttttg gtatttccca aagcgctcaa taatagcgac cgtctgctgc
     3601 ttgacaacgt atagccctga agcaatcaaa atcaaagcaa tcagtacaaa gaacaagaaa
     3661 ctaccaataa aaacaattgg accaagcacc ataatgacct cctaaactag cattttataa
     3721 agcgaatcat tttcatgaag attgatgtac tggggatcaa actcttccaa gcgagaaata
     3781 aactgcgtat aatctgtttt atctcctagg gcaataccga ttagaaccgg ccctgttccc
     3841 ttattggcac gtttgatata ttcaaaacga gtaatatcat catttggccc tagaatatca
     3901 tttacaaatt cacgaagagc ccctggacgt tgtggaaaat taacaacaaa gtagtgttta
     3961 attccctcgt agataagcgc acgctcctcc atttccggca tcctgttgat gtcgttatta
     4021 ccaccagaaa tgatacaaca aatggtttgc cccttgattt tgtccttgac tacctcaaga
     4081 gctgcaatcg ttgctgctcc tgctggctca gcaacaatac ccagcttaga atagaggtct
     4141 aaaatagtcc cggaaatcca tccttcatcc acaccaatca ggcgatctac gtatttccga
     4201 gccacttcgt aggtcgactt accgactttc tgtaccgcaa taccgtcagc aaatttatca
     4261 atttggtcta atttaaccgg acgaccttta tcaaaagccg ctttcattga ccgtgcccca
     4321 cttgcttcaa caccaacaat cttaatttca ggtgcatgtt ctttcagata ggccgaaacg
     4381 cctgcaacca gaccacctcc tcctatggga actaaaattt ggtcaaaact aatggtttgc
     4441 tcttccgctt cttccaagat ttcataggct actgttccct gaccagcctg cacattttca
     4501 tcatcgaatg ggtctataaa tgttttccca ctttcctgag tataatcttg tgctgccttg
     4561 gctgactcat cgaaggtatc acctacgagc cgaatctcta cgtagtctcc tccgaaaaat
     4621 ttaacctgcc ctattttttg ttgcggagtt gtaatgggca taaaaatcgt agcaggaatt
     4681 tgcatttcct gacaggtgta ggccacacct tgagcatggt ttccggccga agcacagacc
     4741 actccgcgtg acttctcatc atctgtcaat tgtgaaatag cataataggc tcctcgaatt
     4801 ttaaaagagc gaacacgctg ttcattttct ctttttaaat aaatagttgc accatatttt
     4861 tccgataagt atcgactata atccaagggg gtacgtacta ccacgcgctt aagaacggag
     4921 tatgcttgtt ctacattttt tgctgtaatc atagttccat taatt
//
LOCUS       DAZQCJ010000117         4954 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000117 DAZQCJ010000000
VERSION     DAZQCJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.119"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            14..2281
                     /locus_tag="ACHU66_001913"
     CDS             14..2281
                     /locus_tag="ACHU66_001913"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HFR3390172.1"
                     /translation="MRFNQFSFIKKETSVYLQELDALGFQLIPNVSSKTNLETFVRKC
                     HFLTANTDFALSNMIADWDTDLLTFFQSDRELTDLIFYQVAFQLLGFVPGVDYTDVMD
                     FVEKSNFPVIYGDMIDNLYQLLATRTKSGNTLIDQLVSDDLIPEDNRYHFFNGKSLAT
                     FSTKNLIREVVYVETPVDTAGTGQTDIVKLSILRPHFDGKIPAVITNSPYHQGVNDVA
                     SDKALHKMEGELAEKQVGTIQVKQASITKLDLDQRELPVSPSTEKLGHITSYSLNDYF
                     LARGFASLHVSGVGTLGSTGYMTSGDYQQVEGYKAVIDWLNGRTKAYTDHTRSIEVKA
                     DWANGKVATTGLSYLGTMSNALATTGVDGLEVVIAEAGISSWYDYYRENGLVTSPGGY
                     PGEDLDSLTALTYSKSLQAGDFLRSKEAYEKGLAAERAALDRTSGDYNQYWQDRNYLL
                     QADKVKCEVVFTHGSQDWNVKPIHVWNMFHALPSHVKKHLFFHNGAHVYMNNWQSIDF
                     RESMNALLSQKLLGYENNYQLPTVIWQDNSGEQTWTTLDTFGGENEAVLPLGTGSHTI
                     ANQYAQEDFDRYGKSYPAFHQDLYAGKGNQVSIDLPVTEDLLLNGQVTLKLRVASSVA
                     KGLLSAQLLDKGNKKRLAPIPAPKARLSLDNGRYHAQENLLELPYVDMPQRLVTKGFM
                     NLQNRTDLMTVEEVVPGQWMNLTWKLQPTIYQLKKGDVLELILYTTDFECTVRDNSQW
                     QIHLDLSQSQLILPH"
     gene            complement(2561..>4954)
                     /locus_tag="ACHU66_001914"
     CDS             complement(2561..>4954)
                     /locus_tag="ACHU66_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HFR3390173.1"
                     /translation="KKFRYSIRKFKVGVGSVLIATCLLGAGVSTPTAFATTETSTATQ
                     VEAAQPTSETEPTVQITGTVSTENTNSESKVAPEVKEEAPVAEKAETATNSAEQNVVS
                     EPEKSEEPTPAPAALTSEAKEEVSENTEVKSAPLPQFQDILKELERDLEELKVFRDYT
                     GSDYSFQKGLWTYLLEDGQKLMSYVQTLEKDSPKIKENYDKLLDFKNHIRMERLTRQV
                     VPYLEKYSGNEFIRSEYREHLEQSEENGSYASQEGKVFEAYVDSAEQAFDRIVNRVNE
                     LESKNLQKKVPDTAPKVAEKPKLDFKVQTRTTYQFLDFKTKEQEDPNLPLGEKVERQK
                     GERGEESETYQDVIVGDKIVASTLISKKRKDPVDHLIAKGTLVEKHPDTAPTAPEKPK
                     LDFTVKERVEREILDYMVEEQDDASLPLGQTKVLREGQKGELVKKYQDVIVEGKVIES
                     KLLSEDRKTPVNRLIAKNILVEKHPDTAPTAPEKPKLDFTVKERVEREILDYMVEEQD
                     DASLPLGQTKVLREGQKGELVKKYQDVIVEGNVIASNLLSEDRKAPVNRLIAKGTLVE
                     KTPDAPAPKAEEEVPASTPMPETPMDKPKTDKVESDKQMPEAKQPEMEQPKAEDMPKA
                     EQPKAEDSAPKTAVPEVAPKTAEKPKLDFTTKERKVEEALPIKEEIRYDASLALGKSY
                     LLQEGKAGKKVSVYQDVIVDGKIVATNLLSETVVEGQNRILVKGSLEMKKEEVKSTPS
                     VQSNLTLSHKGAPSANKATLPATGEQRNNLALVGLGLAGISLAVVATAINKKSKDQI"
BASE COUNT         1227 a         1158 c         1042 g         1527 t
ORIGIN      
        1 aagaggagaa tttatgcgct ttaatcaatt ttctttcata aaaaaagaga cctccgtcta
       61 cttgcaagaa cttgatgctc taggatttca actgattcct aatgtaagta gtaagacaaa
      121 tttagaaacc tttgtcagaa agtgccattt cctgacagca aacaccgact ttgccctgtc
      181 aaacatgatc gcagattggg acacagacct gctgaccttc ttccagtctg accgagagct
      241 gacagacctg attttctatc aagtggcctt tcagttgcta ggcttcgtgc ctggtgtaga
      301 ttatacagat gtcatggatt tcgtggagaa aagcaatttc cctgtcatct atggagatat
      361 gattgacaac ctctatcaac tgctggctac tcgtaccaag tctggaaaca ccttgattga
      421 ccagctggtc agcgatgacc taatcccaga agacaatcgc taccacttct tcaacggcaa
      481 atctttggcg accttttcaa ccaaaaacct tattcgtgag gtggtctatg tcgaaacgcc
      541 tgtcgataca gctgggactg ggcagacaga cattgttaag ctgtccattc tccgtccgca
      601 ctttgacgga aaaattcctg cggtgattac aaacagtcct tatcatcagg gagttaacga
      661 tgtagccagc gacaaggccc tgcataagat ggaaggggaa ctggctgaaa aacaagttgg
      721 taccattcag gtcaagcagg ccagcatcac caagttagac ttggaccagc gcgagctccc
      781 tgtcagccct tccaccgaaa aactgggcca catcacttca tactccctca atgactattt
      841 tcttgctcgt ggtttcgcca gcctccatgt gtctggtgtc ggtacgctgg gctcgacggg
      901 ctacatgaca tctggcgact accaacaggt ggagggctat aaagcggtga ttgactggct
      961 gaacggtcgc accaaggcct acacagacca cacccgctcg attgaggtca aggccgattg
     1021 ggccaatggt aaggtggcga cgacgggact gtcttacctc ggcaccatgt ccaatgcctt
     1081 ggcaacaact ggcgtggacg gtttggaagt tgtcatcgca gaagcaggga tttcctcttg
     1141 gtatgactac taccgtgaaa acgggctggt gaccagccct ggcggctatc cgggcgagga
     1201 cctggacagc ttgactgccc tgacctactc gaaaagtctg caagcagggg atttcctccg
     1261 cagcaaagaa gcctacgaaa aaggcctggc tgccgaacga gctgccctag accgcaccag
     1321 cggtgactac aaccaatact ggcaggaccg caactacctg ctccaggctg acaaggtcaa
     1381 gtgcgaggtc gtatttaccc atggctcaca ggactggaat gtcaagccaa tccacgtctg
     1441 gaatatgttc catgccctgc caagtcacgt caaaaagcac ctcttcttcc acaacggtgc
     1501 ccacgtttat atgaacaact ggcaatccat cgacttccgc gagtccatga atgccttact
     1561 cagccagaaa ttgctaggct acgagaataa ctatcaacta ccaactgtca tctggcagga
     1621 caacagcggt gagcaaactt ggacgacctt ggacaccttt ggcggtgaaa acgaggctgt
     1681 cttgccacta ggtactggaa gccataccat tgccaatcag tatgcccaag aagattttga
     1741 ccgctacggc aaatcctacc ctgccttcca ccaagacctc tatgcaggca agggcaacca
     1801 agtcagcatt gacctgcctg taacagagga cctcttgctt aacgggcagg tcacgctgaa
     1861 actccgtgtt gcttctagtg tagctaaagg tctcttatct gctcagctat tggacaaggg
     1921 aaataaaaaa cgcctagccc caatccctgc gcctaaagca agattgagtc tagacaacgg
     1981 tcgttaccat gcccaagaaa atctgctgga gttgccttat gtggatatgc cacaacgctt
     2041 ggtaaccaag ggctttatga acctgcaaaa ccgcaccgac ctgatgactg tcgaggaagt
     2101 ggtgcctgga cagtggatga atctgacctg gaaactgcag ccgaccattt atcagctgaa
     2161 aaaaggcgat gtcttggagt tgattctcta tacaaccgac tttgagtgta ctgttcggga
     2221 taattctcaa tggcaaatcc accttgattt aagtcaatca cagttaattt taccgcacta
     2281 atactcaatg aaaatcaaaa tcagactagt ttcaaaggtt tgggaaacct ttgaaggttg
     2341 gagatagagt gaacgaagtt cgtttctcca agagcagata tacccctgct actttcttat
     2401 tttatggtag caggctgtca agctccactg gagcttgaca ctcatcaaat caagtcaaca
     2461 acgtctgatt ttgagtttcg aagagtataa tattgatttt cagttctaaa aagaggctgg
     2521 gcgtttccgc cctgcctctt tattgatatt tcataatggt ttagatttga tctttagatt
     2581 ttttgtttat agctgtagcg actactgcca agctaattcc agctaaacca aggcctacta
     2641 aggctaggtt attgcgttgt tcacctgttg caggtagggt tgctttgttt gcagaaggtg
     2701 cgcccttatg actcagtgtc agatttgatt gtactgaagg agttgatttt acttcttctt
     2761 tcttcatttc caagctacct tttacaagaa tacgattttg accttcaaca acagtctctg
     2821 acaataggtt ggttgcaaca attttaccat caactatgac atcttgataa acagatactt
     2881 ttttacctgc ttttccttct tgaagaaggt atgatttgcc aagcgctagg cttgcatcat
     2941 atctgatttc ttctttgata gggagagctt cttctacctt gcgttctttt gttgtgaagt
     3001 ctagctttgg tttttccgct gtttttggtg cgacttctgg aactgctgtc ttaggtgcag
     3061 agtcttccgc ttttggttgc tcagcctttg gcatgtcttc tgctttcggt tgctccattt
     3121 ctggttgctt agcctctggc atctgtttgt cagactctac tttatcagtt ttcggtttgt
     3181 ccattggagt ttctggcatt ggagttgagg ctggaacttc ttcctctgct ttcggcgctg
     3241 gagcatctgg cgttttttcc actaaagtgc cttttgcaat cagacggttg actggggctt
     3301 tacggtcttc tgaaagaaga ttacttgcta tgacattccc ttcaacaatc acatcttggt
     3361 atttcttaac caattctcct ttctgacctt cacgaagtac ctttgtttga ccgagtggca
     3421 aactagcatc atcttgttct tcaaccatat aatctaaaat ttctcgctct actcgttctt
     3481 taacggtaaa gtctaattta ggtttttcag gagctgtcgg tgctgtatca ggatgtttct
     3541 ctactaaaat gttttttgca atcagacggt taactggggt cttacggtct tctgaaagaa
     3601 gtttactttc aatgacttta ccttcaacaa tcacatcttg gtatttctta accaattcac
     3661 ctttttgacc ttcgcgaaga acctttgtct gaccaagtgg caaactagca tcatcttgtt
     3721 cttcaaccat ataatccaaa atttctcgct ctactcgttc tttaacagta aagtccaatt
     3781 ttggtttttc aggagctgtc ggtgctgtat cgggatgttt ctctaccaaa gtacctttag
     3841 caatcagatg atcaactgga tctttacgtt ttttggaaat gagggtactt gcaacaattt
     3901 tatccccaac aatcacatct tggtaagtct cagactcttc gcctctctca cctttttgtc
     3961 gttccacttt ctcaccaagt ggcaagtttg gatcttcttg ctctttcgtt ttgaaatcta
     4021 aaaattgata tgttgttcgt gtttgaactt taaaatctag ttttggtttt tcggctactt
     4081 taggcgctgt atcaggaact ttcttttgga ggttcttgct ctctagctcg ttgactctgt
     4141 ttacaattct atcaaatgct tgttctgcac tatcaacata cgcttcaaaa acttttcctt
     4201 cctgactagc ataactacca ttttcttctg attgttctaa gtgctctctg tattcacttc
     4261 taataaactc attaccagag tacttttcaa gatatggaac aacttgacgt gtcaaacgct
     4321 ccatcctgat atggttttta aagtcgagta atttatcata attttctttg attttaggac
     4381 tatctttctc aagggtttgg acataactca ttagtttttg cccgtcttct aaaaggtatg
     4441 tccatagtcc tttttgaaat gaataatcag agccagtgta gtctctaaat acctttaatt
     4501 cttccaaatc tcgttctaac tcttttagaa tatcctgaaa ttgtggaagg ggagcagact
     4561 taacttcggt attctctgat acttcttcct tagcttcgct tgtgagggct gctggagcag
     4621 gagttggttc ttctgatttt tcaggttcag agactacatt ttgttctgca gagttcgttg
     4681 ctgtttcggc tttttcagca actggcgctt cttccttaac ttctggcgca acttttgatt
     4741 cagaatttgt attctctgta cttactgttc cagtaatctg gactgttggc tctgtttcac
     4801 tcgttggctg agctgcttca acttgagtag ctgttgaagt ctctgtcgtc gcaaaagcgg
     4861 ttggtgtcga gactcccgca ccaagtaagc aagtagcaat cagcaccgag cctacgccga
     4921 ctttaaattt acgaatacta tatctgaatt tttt
//
LOCUS       DAZQCJ010000118         4941 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000118 DAZQCJ010000000
VERSION     DAZQCJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.120"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            313..2964
                     /gene="adhE"
                     /locus_tag="ACHU66_001915"
                     /gene_synonym="adhC"
     CDS             313..2964
                     /gene="adhE"
                     /locus_tag="ACHU66_001915"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR3390174.1"
                     /translation="MADKKVVSPEEKLVEARKHVDELVQKGLVALDEFRKLGQEEVDY
                     IVAKASVAALDKHGELAMHAFEETKRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DEVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLIALKTRNPIVFAFHPSAQESSAHA
                     ARVVYEAAVAAGAPENCIQWITKPSMEATSELMKHDGIATILATGGNAMVRAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEVYDEFVTE
                     FKSYKTYFVNKKEKALLEEYCFGVKANSKNCAEGKLNADIVGRPAAWIAEQAGFSVPE
                     GTNILAAEVAEIGEKEPLTREKLSPVIAVLKVEGRAEGLEAARQMVEFHGLGHSAAIH
                     TEDAELAKEFGTIVRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSISDNVS
                     AMNLLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDRAMVELGF
                     LDRIIEQLDLRRNKVVYQIFSDVEPDPDITTVYKGTELMRTFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPTVDFHDLVQKFMDIRKRAFKFPELGKKSKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPAHVTADTGMDVLTHATEAYVSTVA
                     NDFTDGLALQAIKLVFENLESSVKNADFVSREKMHNASTMAGMAFANAFLGISHSMAH
                     KIGGRFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYKADEKYQDIARLLGLPC
                     STPEEAVAAYAQAVYDLGERIGIQMNLKAQGIDEKELKEHSRELALLAYEDQCTPANP
                     RLAMVDHMQEIIEDAYYGYKERPGRIK"
     gene            3379..4863
                     /gene="thrC"
                     /locus_tag="ACHU66_001916"
     CDS             3379..4863
                     /gene="thrC"
                     /locus_tag="ACHU66_001916"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR3390175.1"
                     /translation="MTLVYQSTRDAKNTVSASQAILQGLATDGGLFTPISIPAVDLDF
                     SVLKDASYQEVAKLILSAFLDDFTADELDYCINNAYDSKFDTPAIAPVVKLKGQYNLE
                     LFRGSTIAFKDMALSILPYLMTTAAKKHGLENEIVILTATSGDTGKAAMAGFADVPGT
                     QIIVFYPRDGVSKVQELQMTTQTGANTHVVAIDGNFDDAQTNVKRMFNDEALRAKLAA
                     KKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIAAGDKVNFTVPTGNFGNILAAY
                     YAKQIGLPVGKLICASNDNNVLTDFFSTGVYDKNRTFRVTTSPSMDILVSSNLERLIF
                     HLFDNDAAKTAELMEALNTAGQYDIQGADADILSLFAAAFATEEETAAEIKRVYDESD
                     YIEDPHTAVASAVYKQYVEQTGDQTPTVIASTASPYKFPVVAVEAVTGQSGLSDFEAL
                     AKLHEISGVALPPAVDGLETAPVRHNTVVAAADMQAEVERYLGV"
BASE COUNT         1457 a         1042 c         1080 g         1362 t
ORIGIN      
        1 atcacgtaat tactacaaaa ttttcaaaaa aatctattta catggaagcg ttttcttgat
       61 aaactagtat cgtaaatgtt agagaaagaa taaatgataa agatttgtct tactttatgt
      121 attttttgac caaacaaagc agaaacaaat ctgaatgttt tctgaaaata ggaaaaagta
      181 agcgctttct ataacgaaac agaaaaaatg tgaaaaaatt aacaatttcc ctttacaaat
      241 aaaataaaaa atagtagaat aacattgtga aaaagttaac aaatagttag cgagtatatt
      301 tggaggaaca caatggctga taaaaaagta gtatcaccag aagaaaagtt ggtagaagcg
      361 cgcaagcacg tcgatgagct tgttcaaaaa ggtttggttg ctcttgatga gttccgtaaa
      421 cttggtcaag aagaagtaga ctatattgta gcgaaagctt cggttgcagc ccttgataaa
      481 catggcgaat tggcaatgca cgcctttgaa gaaactaagc gtggtgtatt cgaagacaag
      541 gctactaaga acttgtttgc ttgtgagcac gttgtcaata acatgcgtca cacgaaaact
      601 gttggtgtta ttgaagaaga tgaagtgaca ggtttgactt tgattgctga gccggtcggt
      661 gttgtttgtg gtatcacacc aacaacaaac ccaacttcta cagcaatctt taaatcattg
      721 attgccttga agacacgtaa tccgattgtc tttgccttcc acccatctgc acaagaatct
      781 tctgctcatg cagctcgtgt tgtctatgaa gcagctgtgg cagctggtgc tcctgaaaac
      841 tgtatccagt ggattacaaa accatctatg gaagcaacat ctgagttgat gaaacatgat
      901 ggtattgcaa caatccttgc aactggtggt aacgcaatgg tgcgcgcagc ttactcttgt
      961 ggtaaacctg cccttggtgt aggtgcaggt aacgttccag cctatgttga aaaatcagca
     1021 aatattcgtc aggcagcaca tgacatcgtt atgtctaagt catttgacaa cggtatggtc
     1081 tgtgcgtcag aacaagcagt tatcattgat aaagaagttt acgatgagtt tgtaacagag
     1141 ttcaaatctt acaaaactta cttcgttaat aagaaagaaa aagctcttct tgaagaatac
     1201 tgcttcggtg tgaaagctaa cagcaaaaac tgtgctgaag gtaaattgaa cgcagacatc
     1261 gttggtcgcc cagctgcatg gatcgctgag caggccggct tctcagtacc agaaggtaca
     1321 aacatcttgg cagctgaggt tgctgaaatc ggtgaaaaag aaccattgac tcgtgagaaa
     1381 ttgtcacctg ttattgccgt cttgaaagtt gaaggtcgtg cagaaggtct tgaagcagct
     1441 cgccaaatgg ttgaattcca cggtctcggt cactctgcag ctattcatac ggaagacgca
     1501 gaattggcga aagagtttgg tacaatcgta cgtgctatcc gtgttatttg gaactctcca
     1561 tctactttcg gtggtatcgg tgatgtttac aacgcattct tgccatcatt gacacttggt
     1621 tgtggttctt acggacgcaa ctcaatcagt gataacgtaa gtgctatgaa cttgctcaac
     1681 atcaagaaag taggaagacg tagaaataat atgcaatggt ttaaagttcc ttctaaaaca
     1741 tacttcgaac gtgactctat ccaatatctc caaaaatgcc gtgacgttga acgtgtcatg
     1801 atcgttacag accgtgccat ggtagaactt ggattcctag atcgtatcat cgaacaattg
     1861 gaccttcgtc gtaataaagt tgtttatcaa atcttctcag acgttgagcc agatccagac
     1921 atcacaacgg tttataaagg tacagaattg atgcgtacct tcaaaccaga tacaatcatc
     1981 gcactcggtg gtggttcacc aatggatgct gccaaagtta tgtggctctt ctacgaacaa
     2041 ccaacagttg atttccatga cctagttcaa aaattcatgg atatccgtaa acgtgccttc
     2101 aagttcccag aacttggtaa gaaatctaag tttatcgcga ttccaactac atcaggtaca
     2161 ggttcagaag taacaccgtt tgccgtaatc tctgataaag caaacaaccg taaataccca
     2221 atcgctgact actcattgac accaactgtg gcaatcgttg acccagcact cgtattgaca
     2281 gtacctgcac acgttacagc ggatactggt atggacgtat tgactcacgc tacagaagca
     2341 tacgtttcaa cagtagccaa cgactttaca gatggtttag cacttcaagc tatcaaacta
     2401 gtatttgaaa accttgaaag ctcagtgaag aatgctgact ttgtatcacg tgagaaaatg
     2461 cacaacgcat caactatggc aggtatggcc ttcgccaacg cattcctagg tatttctcac
     2521 tcaatggctc ataagattgg tggacgtttc cataccgttc acggtcgtac aaacgctatc
     2581 ttgcttccgt acgttatccg ctacaacggt actcgtccag ctaagacagc tacatggcct
     2641 aagtacaact actataaagc agatgaaaaa taccaagaca tcgctcgtct tctaggtttg
     2701 ccatgttcaa caccagaaga agctgttgca gcatacgcac aagcagttta tgacctcggt
     2761 gaacgcatcg gtatccaaat gaacttgaaa gcacaaggta tcgacgagaa agaactcaaa
     2821 gaacactctc gcgaattggc tcttcttgcc tacgaagatc aatgtacccc agctaaccca
     2881 cgtctagcaa tggttgacca catgcaagaa atcatcgaag atgcctacta cggttacaaa
     2941 gaacgtccag gtcgcatcaa gtaaggattt tcgtcacgat acatcgttaa agctccttgc
     3001 ctaactttag ttatgtctac gtcgctttgc cttgtctcgt ttagaaaatc attccttagt
     3061 gatttagctt caccttcgtc aatctgccta gaaagactta ggactagaaa aaagtctagg
     3121 taggagtagc aaatcgtatc ctactcagtt gaacggagta tgatttatag gtaaattgcc
     3181 tccaaatatc gtaagacaat cctctattga aaaatagggg attgtttgtt tagaaataat
     3241 ggtggagatc ctgtaaaaag cgaaagtggt tggaaagtta gggtttagcc gagaaaaaga
     3301 gacttttcta tctatctttc acaattttct gtcaatttgt ggtagaatag aaaaaataga
     3361 ttttttatga gggataccat gacattagta tatcaatcaa cacgcgatgc taaaaatact
     3421 gtatcggcta gtcaggcgat attgcagggc ttggcgaccg acggtggttt gtttacaccg
     3481 atttctattc cagcagttga cttggacttt tctgttctca aagacgcttc ttatcaagaa
     3541 gttgccaagt tgattttgtc ggctttcttg gacgatttta cggcagatga gttggactac
     3601 tgtatcaaca atgcctacga tagcaagttt gacacaccag ctattgcccc agtggttaag
     3661 ctaaaaggtc agtacaattt ggaactcttc cgaggttcaa ccattgcctt taaggatatg
     3721 gctctgtcca tcctccctta cttgatgacg actgcggcga aaaagcatgg tttggaaaat
     3781 gagattgtca ttttgaccgc gacttcaggc gatacaggta aggcagctat ggctggtttt
     3841 gcagacgttc ctggcacgca aatcattgtc ttctacccac gtgacggggt atctaaggtc
     3901 caagaattgc agatgactac gcagacaggg gctaatacgc acgtggtggc cattgacggt
     3961 aactttgacg acgcccagac aaatgtcaaa cgtatgttca acgatgaggc tctccgtgcc
     4021 aagttggcag cgaagaaact tcagttttca tctgccaatt ctatgaacat cggccgcttg
     4081 gtgccacaga ttgtttacta tgtctatgcc tacgctcagc tggttaagac tggcgagatt
     4141 gcggcgggcg acaaggtcaa cttcaccgtt ccaacaggaa actttggaaa catcttggcg
     4201 gcttattacg ccaaacaaat cggtctgcca gttggcaagc tcatctgtgc ttccaacgac
     4261 aacaatgtcc taaccgactt cttctcgact ggcgtttacg acaagaaccg taccttccgc
     4321 gtgaccacca gtccgtccat ggacatcttg gtgtcttcta acttggagcg cttgattttc
     4381 catctctttg acaatgacgc tgcaaaaaca gcggagctga tggaagcctt gaacacagct
     4441 ggtcagtatg atattcaagg ggctgacgcg gatatcctct ctctctttgc tgccgccttt
     4501 gcgacggaag aagaaacggc tgcggaaatc aagcgagtct atgacgagtc agactacatt
     4561 gaagacccac atacggctgt tgcctcagct gtctataaac agtatgtgga gcaaacaggg
     4621 gaccaaactc cgactgtgat tgcttctaca gcaagtcctt acaagttccc agtagttgcg
     4681 gttgaggctg tgacaggcca gtcaggtttg tctgactttg aagcccttgc aaaattgcat
     4741 gagatttcag gtgtagcttt gccaccagct gtggacggct tggaaacggc acctgtccgc
     4801 cacaatactg ttgttgcggc agccgatatg caggctgaag tagagcgtta tttgggtgtt
     4861 taatttcgat ttattttatg acaaactgct cattttgaac agtttgtttt ctttttgctg
     4921 tttaaattaa aatttacaga t
//
LOCUS       DAZQCJ010000119         4782 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000119 DAZQCJ010000000
VERSION     DAZQCJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.121"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            95..1243
                     /gene="cps2T"
                     /locus_tag="ACHU66_001917"
                     /gene_synonym="wchF"
     CDS             95..1243
                     /gene="cps2T"
                     /locus_tag="ACHU66_001917"
                     /gene_synonym="wchF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta 1-4 rhamnosyltransferase Cps2T"
                     /protein_id="HFR3390176.1"
                     /translation="MKHVFIIGSRGLPAKYGGFETFVQQLVSHQQSEKICYHVACLSD
                     TSHHQHSTYLGADCFTINPPKLGPARVIAYDMMAITYALKIIKEQQMERPIFYILGNT
                     IGGFIVPFAKQIHSVGGTLFVNPDGLEWKRAKWAKPVQRYLKFSEKMMVKYADLIIAD
                     NEGIEDYIQSEYSATNTRFIAYGTDLSPTKLTKESEQVRQYFTNWNIKEKEFYLIIGR
                     FVPENNYVTAIKEFMASNTRRDLVIVANHEGSAYFQKLKDETGFISDKRIKFVGTVYD
                     QELLKYLRQECRAYIHGHEVGGTNPSLLEALAQTNENLVLGVDFNRKVALEGARYWSK
                     ESGSLSQLINQIDENAEAVELGEIAKKHMKDEYTWEKIVQEYEELFLQ"
     gene            1240..2169
                     /locus_tag="ACHU66_001918"
     CDS             1240..2169
                     /locus_tag="ACHU66_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR3390177.1"
                     /translation="MKVNILMSTYNGQQFLAEQIRSIQEQTYTDWTLFIRDDGSSDRT
                     REIIKDFVEQDQRIHFIDVETDENLGVIKSFHRLIHYDTADFYFFSDQDDVWLPNKLE
                     VSLKKAQAYPTNLPLMVYMDLKVVNQNLEVMAESMVKSQSHHANTELVQELTENTVTG
                     GVAMINHHLAEMWQVTEDILMHDWYLALLASALGKLVFIDKPGELYRQHADNVLGART
                     LSKRFKKWIRPHILFAVYWDLIKNSQKQARHLLQMPLSQSNRELIEAFVTIMDKPMLE
                     RFRILRKYGLRKNKTFHTLVFTTLIVTKFAYKE"
     gene            2172..2978
                     /locus_tag="ACHU66_001919"
     CDS             2172..2978
                     /locus_tag="ACHU66_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR3390178.1"
                     /translation="MNLLNKENQILLREMIKTDFKLRYQGSLIGHLWSILKPMLLFTI
                     MYLVFVRFLRFDDGTPHYAVGLLLGMVTWNFFTEATNMGMMSIVSRGDLLRKLNFSKE
                     IIVFSSVAGAAINYAINLMVVFVFAAINGVPFTWSILIIIPLFFELALLATGIAFILS
                     SLFVRFRDIGPIWEVFLQAGLYATPIIYSLTFILQRGQVTFAKLMMLNPVAQIIQDLR
                     HFIVYSGNTTVSELIENPVIVAIPYILPVVVYLLGITVFRKNAKKFAEIL"
     gene            2978..4192
                     /locus_tag="ACHU66_001920"
     CDS             2978..4192
                     /locus_tag="ACHU66_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3390179.1"
                     /translation="MVKNNNIAVKVEHVSKSFKLPTESSQSLRTTLVNLFKGIKGYVE
                     YNVLQDISFEVEKGDFFGIVGRNGSGKSTLLKILSQIYVPERGTVAVDGKLVSFIELG
                     VGFNPELTGKENVYLNGAMLGFTAEEIDAMYDDIVEFAELSEFMNQKLKNYSSGMQVR
                     LAFSVAIKAQGDILILDEVLAVGDEAFQRKCNDYFLERKKSGKTTILVTHDMGAVKKY
                     CNKALLIEKGYVKALGEPDDVANQYSFDNVLASISEVTENEEPLHQSDIVKDLQINLV
                     SKNQIEPDESIEIEFRYTVLEDIETHVAFTFLDLERHFDVYNDNSMDLKTFGKGEKFF
                     RVKCKLPSINQAKLKMAVSVRNSNKQPLLFAKTSDTPAIFISRKFSTDNIAEEDAATG
                     FIQRNSQWELLD"
     gene            4174..>4782
                     /locus_tag="ACHU66_001921"
     CDS             4174..>4782
                     /locus_tag="ACHU66_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HFR3390180.1"
                     /translation="MGAARLIKYNGGRMSRLLVYVHYNKYNVVSEYIYFQLKSIRSIY
                     SDIVFVSNSHVSKDIVQYLQSERLIDFFIQRDNIGYDFAAWKEGLNQVTFYQYDSVTL
                     MNDTCFGPLWDLEDYYSQFDSDVDVDFWGMTNHLETKIDSVVVPEHLQSYFMVFKKQI
                     LQSQAFVGFWSSVSELTDIQDVIKLYESQLTKILLSEGYSYKC"
BASE COUNT         1565 a          709 c          984 g         1524 t
ORIGIN      
        1 aagaaaagcc tttataacca aactaggata gaattaggaa aaaaaactaa aaaatggtat
       61 aatgaaaaga actgcaattt gatggagagg attcatgaaa cacgttttta ttatcggtag
      121 tcgtggactg cctgcaaaat atggtggctt tgagaccttt gtccaacaat tggtcagtca
      181 ccagcaatct gagaagattt gttatcatgt ggcttgttta tcggatacaa gtcatcacca
      241 acattcgacc tacctaggtg cagattgctt caccattaat ccaccaaaac ttggaccggc
      301 tcgtgtgatt gcttatgata tgatggcgat cacttatgca cttaaaatca ttaaggaaca
      361 gcagatggag cgaccaattt tctatatctt gggaaatacc ataggtggct tcatagtacc
      421 ttttgcgaaa cagattcact ctgttggagg taccttgttt gtcaatccag atggtttgga
      481 atggaaacgt gccaagtggg ctaagccagt ccaaaggtat ctaaaatttt ctgaaaaaat
      541 gatggtcaaa tatgctgatt tgattattgc tgataatgag ggaattgagg attatattca
      601 atctgaatac tcggctacca atacacgctt tattgcctat ggtacagatt taagtccgac
      661 taagttaaca aaagaatctg aacaagttcg ccaatatttt acaaattgga acattaaaga
      721 gaaagaattt tatctgatta tcggtcgttt tgtacccgag aataactatg tgacagcaat
      781 taaggagttt atggcaagca acacgagacg tgatttagtc atcgttgcaa accatgaagg
      841 ctctgcctat tttcaaaaat tgaaagatga gacaggcttt atatccgata agcggattaa
      901 gttcgtagga acagtatatg accaagaact acttaagtat ctgcgccaag aatgtcgagc
      961 ttatattcat ggacatgaag ttggaggaac caatcctagt ctcctagaag cgctggcgca
     1021 aaccaatgaa aatttggttt taggtgttga ttttaaccgc aaagttgccc tagaaggtgc
     1081 tcggtattgg agtaaggagt caggtagcct gagtcaatta attaaccaaa ttgatgagaa
     1141 tgctgaagcg gtggaattag gagaaattgc gaaaaaacac atgaaggatg aatacacctg
     1201 ggaaaaaatc gtccaagagt acgaggaatt atttttacaa tgaaagtaaa catactcatg
     1261 tcgacctaca atggtcaaca gttcttagct gagcaaattc gctctattca agaacagact
     1321 tacacagact ggacactgtt cattcgtgat gatggttcca gtgatcgaac tagagagatt
     1381 attaaagatt ttgttgaaca agatcagcga attcacttca tagatgttga gacagacgaa
     1441 aatctcggtg tcatcaaaag ttttcatagg ctaattcatt atgatactgc tgatttctat
     1501 tttttcagtg accaagatga tgtttggcta ccaaataaac tggaagttag cctgaaaaaa
     1561 gcacaagcct atccaacaaa tttgccattg atggtttata tggacctcaa agttgtcaat
     1621 caaaatctag aagttatggc agaaagcatg gtaaaatctc aatctcatca tgccaatacg
     1681 gaattggtgc aagagttgac tgagaatact gtaactggcg gtgtcgccat gattaatcat
     1741 catcttgctg aaatgtggca agtaactgag gatattttga tgcatgactg gtatttagct
     1801 ttgttggcct cggctttggg taaactggtt tttattgaca agcctggcga actctatcgc
     1861 cagcatgcgg ataatgtttt gggagctaga accctttcta aacgatttaa aaaatggatt
     1921 cgtcctcaca ttttatttgc agtttattgg gacttgatta aaaatagtca aaaacaagcc
     1981 cggcatttat tacaaatgcc cctttctcaa tcaaatagag aattaataga agcctttgta
     2041 accattatgg ataaaccaat gcttgaacga ttcaggattt tgagaaaata tggtttaaga
     2101 aaaaacaaaa cctttcatac attagtattc acaacactga ttgtgacaaa atttgcatat
     2161 aaggaataac aatgaattta ttaaataaag aaaatcaaat actcttaaga gagatgatta
     2221 aaacagattt taaattacgt taccaaggtt ctctcattgg gcatctttgg tcgattttga
     2281 aaccgatgtt actttttaca attatgtatt tagtatttgt tcgttttttg cgctttgatg
     2341 atggaactcc tcattatgca gtagggttat tattaggtat ggtaacctgg aactttttta
     2401 cagaagcaac taatatgggg atgatgtcta ttgtttcacg cggagattta ttgcggaagt
     2461 tgaatttttc taaagaaatt attgtatttt cttcagttgc aggagctgca attaattacg
     2521 ccattaactt aatggttgtg tttgtatttg ctgctattaa cggggttccc tttacttggt
     2581 ccattctaat tataatccca ttgttttttg aattggcatt actagcaaca ggtatagctt
     2641 tcattttatc ctctttattt gtaagattta gagatattgg tccaatttgg gaagtgttct
     2701 tacaggctgg attgtatgca acaccaatta tttattcact aacattcatt ttacagcgtg
     2761 ggcaagtaac atttgctaaa ttgatgatgt tgaaccctgt tgcacaaatt attcaagact
     2821 tgagacattt tattgtttat tctgggaata ctacagttag tgaattgatt gaaaatcctg
     2881 ttattgtggc aattccgtat attcttccag tcgttgttta cctattgggt ataactgtgt
     2941 ttagaaaaaa tgccaaaaag tttgcggaga ttttgtaatg gtaaaaaata ataatattgc
     3001 tgtgaaagta gaacatgtta gcaagagttt taagcttcca acggagagta gtcagagtct
     3061 aagaactact ttagtcaatt tatttaaagg gattaaaggc tacgtagagt ataatgtttt
     3121 gcaggatatc agtttcgaag ttgaaaaagg agattttttt ggaattgttg ggcgcaatgg
     3181 ttctggtaag tctacactgt taaaaatttt atctcaaata tatgttcctg aaagagggac
     3241 tgtagctgtg gatggtaaat tagtttcttt tattgaactt ggtgttgggt ttaatccgga
     3301 actaactggt aaggaaaatg tatacctaaa cggtgcgatg ttaggattta ctgcagaaga
     3361 aattgatgca atgtatgatg atattgttga atttgcggaa ttgagtgaat tcatgaatca
     3421 aaagttgaaa aactattcta gtggtatgca ggtacgttta gccttttcag ttgccattaa
     3481 agctcaagga gatattctga tacttgatga ggttctagca gtcggtgatg aggcctttca
     3541 gcgtaaatgt aatgattact tcctagagcg taaaaaaagt ggaaaaacaa ccattcttgt
     3601 cacccatgat atgggagctg tgaaaaagta ttgtaataaa gctcttctta tagagaaagg
     3661 atacgtcaag gcattaggag aacctgatga tgtagctaat caatacagtt ttgataatgt
     3721 gcttgcctca ataagcgaag ttacagaaaa cgaagaacct ctgcatcaga gtgatattgt
     3781 aaaagatttg cagattaacc tggtctcaaa aaatcaaatt gaaccagatg aatcaattga
     3841 aatcgagttt agatatacgg ttttagaaga tattgaaaca catgttgcat ttacattttt
     3901 agatttagaa cgtcattttg atgtatataa cgacaattct atggatttaa aaacatttgg
     3961 taaaggcgag aagtttttcc gagtgaaatg taagctacct tcaattaatc aggcaaaatt
     4021 aaaaatggcg gtgtcagtac gaaatagtaa taagcagccg ttattatttg ccaagacatc
     4081 tgacacacca gctattttta ttagtaggaa gtttagtact gataatattg cagaggaaga
     4141 cgcagcaacc gggtttattc agagaaatag ccaatgggag ctgctagatt aataaaatat
     4201 aacggaggca gaatgtcgcg tttgttggta tatgttcact ataataaata taatgttgtt
     4261 agtgaatata tttattttca attaaagtcg atacgaagta tttattcaga catagttttt
     4321 gtttctaaca gccatgtttc aaaggacata gtacaatatt tacagtcgga acgattgatt
     4381 gattttttta tccaaagaga taatattggt tatgattttg cggcatggaa agaagggctg
     4441 aatcaagtaa cattttatca atatgattcg gtaactctga tgaatgatac atgtttcggc
     4501 cccctctggg acctagagga ttattatagt caatttgaca gtgatgttga tgtagatttt
     4561 tgggggatga ccaatcactt agagacaaaa atagatagtg tagttgtccc ggaacattta
     4621 cagtcatatt ttatggtatt taaaaaacaa atcttgcaga gtcaagcatt tgttggtttt
     4681 tggtcatcgg taagtgagtt aacagatatt caagatgtta taaaattata tgagagccaa
     4741 ttgacaaaaa tacttttatc agagggttat agctataagt gt
//
LOCUS       DAZQCJ010000120         4744 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000120 DAZQCJ010000000
VERSION     DAZQCJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.122"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..1031
                     /locus_tag="ACHU66_001922"
     CDS             <1..1031
                     /locus_tag="ACHU66_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR3390181.1"
                     /translation="RPIGSFLEIVLLPMILTGLLYYLLNPMVDWMEKHKISRTVGISI
                     LFVLISLLIIWGLAVAIPSIQEQVTSFAQNLPSNIQKIEGQVTGLLQDQRFEQFRPTA
                     LEMLNKVNDQIVAYAQKFSSSAVNWASNLISTASQIIVAILIMPFILFYLLRDGQYLN
                     KHITQYLPTKWREPIGTVLSDVNGQLSNYVRGQVTVAIIVALMFSVMFSIIGLSYPIT
                     LGVMAGFLNLIPYLGSFLAMIPAVILGLIAGPIMLIKVLVVFMIEQTIEGRFVTPLII
                     GSSLSIHPITILFVLLTAGQMYGVLGVLLGIPIYASIKVLVKAAFEWYKAHSSLYEDE
                     ENEVVNEQ"
     gene            1021..2250
                     /locus_tag="ACHU66_001923"
     CDS             1021..2250
                     /locus_tag="ACHU66_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR3390182.1"
                     /translation="MNNSEKMLVCLDQQDLDKADKYFKRALVQDDSETLLSLAAYLEG
                     IGFFPQARQIYEQVKEEFPEVLINLAQIAFEDGLVEEAFRYLEEISEDSSIYVEALLV
                     KADLYQAEGLSDVACEKLLEASYLSDEPIILFGLAELDMELELFNEAISYYAQLDNRE
                     IYELMGVSTYQRIGIAYASLGKFEAAIEFLEKAVELEYDDQTVFELAALLFEREEYQK
                     ANLYFKQLDTINPDFEGYEYAYAQSLHAEHKIDDALVMTQKGLAKNDFDANLLLQASQ
                     YAYELHDEAGAEDYLLRAKEVADDSNELALRLSNLYLEQERFEEVVALFDEEVDNVLA
                     RWNKAKAYQALERDDEAIALYDELAGELAENPEFLADYVAVLRQLGRLDEAKEQASRY
                     IQLVPDDLAMQEFLNEE"
     gene            2254..2970
                     /gene="budA"
                     /locus_tag="ACHU66_001924"
     CDS             2254..2970
                     /gene="budA"
                     /locus_tag="ACHU66_001924"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019322637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFR3390183.1"
                     /translation="MQVNRLFQYNTLGALMAGLYGGSLTVGELLEHGDLGLGTLDSID
                     GELIVLDGKAYQAKGAGEKPEVVEVPADMKVPYAAVIFHEAEVIFKQRFEMTSDELHQ
                     RIESYYDGENLFRSIKIKGTFSHMHVRMIPKSAFDVRFAEVASRQPEYTAENISGTIV
                     GIWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDYVISEGMVEVGPVDQLDQRFPVQDR
                     QYLFAKFNAKEVREDIDKAE"
     gene            complement(2996..4654)
                     /locus_tag="ACHU66_001925"
     CDS             complement(2996..4654)
                     /locus_tag="ACHU66_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HFR3390184.1"
                     /translation="MSFDGFFLHHMTAELKANLEGGRIQKINQPFEQEIILNIRSNRQ
                     SHKLLLSAHSVFGRVQLTQSDFTNPKVPNTFTMILRKYLQGAIIEEIRQLDNDRILEF
                     SVSNKDEIGDHTQATLIVEIMGKHSNIILVDKSEQKIIEAIKHVGFSQNSYRTILPGS
                     TYIRPPETHSLNPYTVSDEKLFEILSTQELSPKNLQQVFQGLGRDTASELASHLQTDR
                     LKNFRAFFDQATQPSLTAKSYAALPFANSPENQPHFESLSSLLDFYYQDKAERDRVAQ
                     QANELIKRVASELEKNRKKLVKQEQELADTETAELVRQKGELLTTYLHQVPNDQASVT
                     LDNYYTGQELEIELDVALTPSQNAQRYFKKYQKLKEAVKHLTNLIEETKATIVYLESV
                     DTMLGQASLAEIDEIREELIETGYLKRRHREKIHKRQKPERYLATDGKTIILVGKNNL
                     QNDELTFKMAKKGELWFHTKDIPGSHVVITDNLDPSDEVKTDAAELAAYFSKARHSNL
                     VQVDMIEAKKLHKPTGGKPGFVTYRGQKTLRVTPTEDKIKSMKI"
BASE COUNT         1228 a          922 c         1142 g         1452 t
ORIGIN      
        1 tcagaccaat aggaagcttt ttagaaattg tattgttacc gatgatttta acaggtcttt
       61 tgtattacct attaaatccg atggttgact ggatggaaaa gcataaaatt tctcgtacag
      121 ttggtatttc cattctcttt gttttaatca gtcttctcat tatctgggga ctggcggtgg
      181 caattccgag tattcaagag caagtgacat cttttgcgca aaatcttcct tcaaatatcc
      241 agaaaatcga aggacaggta actgggctcc tacaggatca gcgctttgaa caatttagac
      301 caacagctct ggagatgtta aataaggtca atgatcagat tgttgcctat gctcagaagt
      361 tttcttcaag cgctgtgaac tgggcaagta atttgatttc aaccgcttca cagattattg
      421 ttgctatttt gattatgccc tttattctct tttatctgtt acgggatggt cagtatttga
      481 ataaacacat tacccaatat ctaccaacca agtggagaga accgattgga acagtactat
      541 cagatgtgaa tgggcaatta tcaaactatg ttcgtggtca ggtgaccgtt gcgattattg
      601 ttgccctgat gttctcagtg atgttctcaa tcattggtct tagctatcca ataactttgg
      661 gggtgatggc tggtttcctc aatctaattc cgtatttggg ttctttcttg gccatgattc
      721 cagctgttat tttaggattg attgctggtc caatcatgct gataaaggtt ttggtagtct
      781 ttatgattga gcagactata gaaggtcgtt ttgtgacacc attgattatc ggaagctccc
      841 taagtattca tccgattacc attttatttg tattattgac agctggtcag atgtacggtg
      901 tcctaggagt tcttctggga attccaatat atgcctctat taaggtttta gtgaaagcgg
      961 catttgaatg gtacaaggca cacagtagtt tgtatgaaga tgaagaaaat gaggttgtga
     1021 atgaacaata gtgaaaaaat gttggtttgt ttggaccaac aggatttaga caaggcagat
     1081 aagtatttca agcgcgcctt ggtgcaagat gatagcgaaa cgcttttgtc cttggcagcc
     1141 tacttagaag gcattggttt tttcccacag gctagacaaa tctatgaaca ggtcaaagaa
     1201 gaatttcccg aagtactgat caatcttgcc caaatagcat ttgaagatgg cttggttgaa
     1261 gaagctttca gatatttgga ggaaatttcg gaagacagtt ctatctatgt ggaagccttg
     1321 ttggtcaagg cggacctgta tcaggcagaa ggcttgtcgg atgtggcgtg tgaaaaattg
     1381 ttagaagcaa gttacttatc agatgaaccc atcatcctct ttggcttagc agaattggat
     1441 atggaactgg agctttttaa tgaggctatt tcctactatg cccagctgga taatcgtgaa
     1501 atctacgaat taatgggagt ttctacctat cagcgaattg gtattgccta tgcaagtttg
     1561 gggaaatttg aagcggcgat tgaatttttg gaaaaagctg ttgagttgga atacgatgat
     1621 cagacggtct ttgaattggc tgctctgcta tttgaacgag aagaatacca aaaggccaat
     1681 ctctatttca agcagttgga taccatcaat cctgattttg agggctatga atatgcctat
     1741 gctcagtctc ttcatgccga gcataagatt gatgatgcct tggttatgac tcaaaaaggc
     1801 ctggctaaaa atgactttga tgccaatctc ttgcttcagg cttcacagta tgcctatgaa
     1861 ttgcacgacg aggcgggggc agaagattat cttctacggg caaaagaagt ggcagatgat
     1921 agtaacgaac tagctctgcg cttgtccaat ttgtatcttg agcaggaacg atttgaagag
     1981 gttgtggctt tatttgatga agaggtagat aacgttctag ctcgctggaa taaggccaaa
     2041 gcctatcaag ctctagaaag agatgatgaa gctattgcgc tttacgatga attggctgga
     2101 gaattggctg agaatcctga atttttggct gattacgtag cggttttacg tcaacttggt
     2161 cgtttggatg aggcaaaaga gcaagctagt cgttatatcc aattggtacc agacgattta
     2221 gccatgcaag aatttttgaa tgaagagtag gttatgcaag taaatcgatt atttcaatac
     2281 aataccttag gtgccttaat ggctggactg tatggcggct ccttgacagt tggcgaattg
     2341 ttggaacatg gtgatttggg tttgggaacc ctggattcca ttgatggcga gctgattgtc
     2401 ttggatggaa aggcttatca ggccaagggt gcaggggaga aaccagaagt ggttgaagtt
     2461 ccagctgata tgaaagttcc ctatgcggcg gttattttcc atgaagcaga agtcattttc
     2521 aaacaacgct ttgaaatgac tagcgatgaa ttgcaccagc ggattgagtc atactatgac
     2581 ggggaaaatc tttttcgctc catcaaaatc aaagggacgt tttcgcatat gcacgtgcgc
     2641 atgattccaa aatcggcttt tgatgttcgt tttgcggagg tggcaagtcg ccagcctgag
     2701 tataccgctg agaatatttc aggtaccatt gttggtattt ggacaccaga aatttttcac
     2761 ggggtcagtg tggctggcta tcatttgcat tttatttcgg atgatttgac atttggcggg
     2821 catgtcatgg actatgtgat ttcagaagga atggttgaag tgggaccagt cgatcaacta
     2881 gaccaacgtt ttcctgttca agaccgccag tatctttttg ctaagttcaa cgctaaggaa
     2941 gttagagaag atattgataa ggcggagtag gtttcaatac agaaaaatcc atgaattaga
     3001 ttttcatgga ttttatttta tcttcagttg gggtgacacg gagggttttc tgacctcggt
     3061 aggtcacaaa gcctggtttg ccacctgttg gcttatggag ttttttggct tcaatcatat
     3121 cgacttggac caaattggaa tgtctggcct tagagaagta ggctgctaat tcagctgcgt
     3181 ctgtcttgac ctcgtcactt gggtccaagt tgtctgtgat gaccacatga ctacctggaa
     3241 tgtctttggt atggaaccag agttcgcctt tcttggccat tttgaaggtc aactcatcat
     3301 tttgcaggtt atttttcccg accagaataa tagtcttccc gtccgtcgcc aagtatcgct
     3361 caggtttctg acgcttatgg attttctcac ggtggcggcg tttgaggtag cctgtttcaa
     3421 tcaattcctc acggatttca tcgatttctg ccagactagc ctgtcccagc atggtgtcaa
     3481 cggactccag atagacaatg gttgccttgg tctcctcaat caagttggtc aggtgcttga
     3541 ccgcctcctt gagtttctgg tactttttga aataacgttg ggcattctgg ctaggagtaa
     3601 gagccacgtc caactcaatc tccagttcct ggcccgtata gtagttgtct aaggtcacac
     3661 tagcctggtc atttggtact tgatgcagat aggtggtcaa gagctcgccc ttttgtcgca
     3721 ccaactccgc cgtctccgta tccgccaatt cctgctcctg cttgaccagt ttcttgcgat
     3781 ttttctccaa ctcactggct acccgcttga tcagctcatt ggcctgctgg gccacccgat
     3841 cccgctccgc cttgtcctga tagtagaagt ccagcagact gctcaggctc tcaaagtgtg
     3901 gctggttttc agggctattg gcaaatggca gagcagcata ggatttagct gtcaggctag
     3961 gctgagttgc ctggtcaaaa aaggcacgga agttcttgag gcggtccgtc tgtaagtgac
     4021 tggccagttc agaagctgta tcccgcccca aaccttgaaa gacctgttgg agattttttg
     4081 gactcagttc ctgagtcgat aagatttcaa acaatttctc atcagacacc gtataaggat
     4141 tgagagaatg tgtctccggt ggacgaatat aggtggagcc tggcaagata gttcggtagg
     4201 agttttgcga gaaaccaacg tgcttgatag cctcaataat cttctgttca gacttatcta
     4261 ccaagatgat attgctgtgc ttgcccatga tttccacaat caaggttgct tgggtatggt
     4321 cgccaatctc gtccttgttg gacaccgaaa attctaagat gcggtcattg tccaactgac
     4381 gaatctcctc gataatggct ccctgcaagt atttccgcag aatcatggta aaggtattgg
     4441 gcactttggg attggtaaag tccgactggg tcagctgaac ccgaccgaaa accgaatggg
     4501 cggacaagag caatttatgg ctctgacggt tgctacggat attgaggata atctcctgtt
     4561 caaagggctg gttgattttc tgaatccgcc caccttctaa attggccttt aactcagccg
     4621 tcatgtgatg taaaaaaaat ccgtcaaaag acatggtttt cctctttcta atagctataa
     4681 ctcagtctag tatatcataa atcagtgact ttttaacgaa ggggactaaa agaagtattc
     4741 ttat
//
LOCUS       DAZQCJ010000121         4684 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000121 DAZQCJ010000000
VERSION     DAZQCJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.123"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            207..4466
                     /locus_tag="ACHU66_001926"
     CDS             207..4466
                     /locus_tag="ACHU66_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HFR3390185.1"
                     /translation="MKKETSKFTKKVILGQLLICTSFLVWAGQTVAAEEVSQGEPVAI
                     NNETPPAIEAKPLVEATPSTETEPSTPEEKQEEPQVADKNVVEGKKPVTNSQHQMVSE
                     LSTVPSVSIKNPDAATDGSAYGSSDDANSNTKIIAGKETGESDNGYSKWDHVYVQYDF
                     EKEVPVHTVKIHRNTYDNAVSTFKDVKVELSTSVDFSPETTEVIFKQADVEETIANKG
                     QPQVIQLPAPINARYIRIWGRGHYIQNTNSSWKGYSNGVLFNEIEVLAQVEADAGTNE
                     EPQTELVNLAKLKKPYFYGKAPTNPEAINDGKFDENYTTQESIGNQAYLQYEFRNVYT
                     IEEIKVQLAPGEYRNFRIDLDDDYNDGNNYNTIYKNENFTIDKEQIITIKTPKNSKAR
                     YVRFRGTRIDDTPLRYSEIQIMGRGKSYDESAPEYIPPKSEYDTLVWSDEFNGDKIDE
                     SKWQIIDGMWNHAAIYNRKAVSIKKDGDKSYLSIRSTNHKDKKTLVEAVGHDRYDDKE
                     LPDKVTWSSGRLESKDKYSFQFGRMAVRAKVNDSKGIWPAIWMLAQDETGHDEIDVLE
                     YLGQNPWDAWTTNHFGILAKNKKSDGSAYKNYEAWSQEFHVYEVEWTPDVIKWYIDGK
                     FVFQTDRGKDRDGMHTRPMFPILETQVGDGWVGDVDYKRNMTKQDSEYLVDWVRIYQK
                     ADQSQVHFDNLDDAKPNDYVIKPQQKLGHLVTVSNGKAAHENKNNFYYGGQPRYETSR
                     LYADGDGENALVYKIANPEALHLTTYYKTLEDYSVYNKEAGANEGKSVRKHLINAKDG
                     EIDFTVYSSVDGKSWNKENVTVVDNFVEATPAYARTTFDIRNIRKGTKFVKIVFPTVK
                     GISYKLEDGTEKGLQASDVQLAKVTFVATHPEAEPEKPIVDQEKLALSKVFSLDAGRK
                     YFSVNQIKEIIDLISSYGYTDMNLLLGNDGLRFLLDDMNLTVGDKVYDSEAVRKAIIN
                     GNKHYYDDPNGTTLSQSEMDDILAYAKEKNISIIPTINSPGHMDAIVEAMEELGIKSP
                     KFSYNGKTSARTVDLKNDQAVAFTKALIDKYASYFAGKSKIFNIGLDEYANDATNASG
                     WHILQTQGDYGKFIQYANDLAAIVKKHGLEPMAFNDGIYYNHDTSSGTFDKDIIASFW
                     TGGWGGYDVASSKSLHEKGHKILNTNDAWYYVIGREAEGLGWYNLDQGLRGTKSTRFD
                     QVPKSENTKVPIIGSMVAVWADDPSKPYSYDSIKKLLHSFTENNSDYFLAEYRDLREE
                     VGKIPTDLNSYTPESVATLKEVLNKIDWKLSRNNQAFVDGYLAEVKAAREALVPVTAK
                     GDSVKAESLPEGKLPLITAKGKSVQAEPRPAFKGYLTSNYGPSVTPLSLVSKPSSEQS
                     RSQQAASQKKELPNTGAVDGLGFSLLGMLGLAFASRRRKEG"
BASE COUNT         1578 a          815 c          995 g         1296 t
ORIGIN      
        1 tagaaataga acccatctgc atatagaata agtcaagtta gactattcta caataaaacg
       61 cttacatgtt atatttgtgt cagaaatgcg atcgcacatc aaccagtaaa ctgaattttg
      121 agtcttatac gagactaaat aggtttaaaa actgctttat tttggctata ggtaggataa
      181 acttgcagac tttataggag gaaataatga aaaaagaaac aagtaagttt acgaaaaaag
      241 tcatccttgg tcagcttctc atctgtacca gttttttggt atgggcaggg cagaccgttg
      301 cagcagagga agtatctcag ggggaaccag tagcaatcaa caatgaaact ccacctgcta
      361 ttgaagcaaa accacttgtg gaagctactc catcaactga aacagaacct tcaactccag
      421 aagaaaaaca ggaggaacca caagtagctg ataaaaatgt ggtagaaggg aaaaagcccg
      481 taactaacag ccagcatcag atggtttcgg aattatcaac ggtcccttct gtttctataa
      541 aaaatccaga tgcagctacc gatgggagtg cttacggtag tagcgacgat gccaatagca
      601 ataccaaaat cattgctggt aaagaaactg gcgaatcaga taacggttac tccaagtggg
      661 atcatgttta cgttcagtat gattttgaaa aggaagttcc cgtccatact gtaaaaattc
      721 atcgtaatac ttatgataat gcagtctcta cttttaaaga tgttaaagtg gaattgtcta
      781 caagtgtaga tttttctcct gaaaccacag aagtcatttt caaacaggca gatgtagaag
      841 aaacaattgc aaataaagga caacctcaag ttatccaatt accagcacca atcaatgctc
      901 gttatattcg tatttgggga cgggggcatt atatccaaaa tacaaacagt tcatggaagg
      961 gatatagtaa tggcgttctc tttaacgaga tagaagtctt ggcgcaggtt gaagcagatg
     1021 ctggtacgaa tgaagaacca caaaccgagc tagtcaacct cgctaaattg aaaaaaccat
     1081 atttctatgg taaggctcca actaatccag aagccattaa cgatggaaag ttcgacgaga
     1141 attatactac tcaagaatca atagggaacc aggcctatct tcaatatgaa tttagaaatg
     1201 tctatactat tgaggaaatc aaggttcagt tagctcctgg agagtatcgt aatttccgga
     1261 tagatttgga tgatgattat aatgatggta ataattataa tacaatctat aagaatgaaa
     1321 atttcaccat tgacaaggaa cagatcataa cgattaaaac tccaaaaaat tctaaggcgc
     1381 gttatgttcg ctttagaggt acgcgcatag atgatactcc tcttcgctat tcagaaattc
     1441 agattatggg acgaggaaaa tcttatgacg aatctgctcc agaatacatt ccaccaaaat
     1501 ctgaatacga tacattagtc tggtctgatg aatttaatgg cgacaagatt gatgaaagta
     1561 aatggcaaat tattgatggc atgtggaatc atgcggctat atacaatcgt aaagcagttt
     1621 ctatcaaaaa agacggcgac aagagctatt tgtctatccg tagcacgaac cataaggata
     1681 aaaaaacttt ggttgaagca gttgggcacg atcggtatga tgataaagaa ttaccagata
     1741 aagtaacatg gtcttctggt cgcctagaat ctaaagataa gtattctttc caatttggtc
     1801 gaatggctgt tcgagctaag gtaaatgact ctaagggaat ctggcctgct atttggatgt
     1861 tggcacagga tgagaccggg catgacgaaa ttgacgtttt ggaatacctt ggtcaaaatc
     1921 catgggatgc atggacaact aaccattttg gtattttggc aaaaaataaa aaatctgatg
     1981 gctcagctta taagaattac gaagcttgga gtcaggaatt ccatgtctac gaagtcgaat
     2041 ggactccaga tgtcatcaaa tggtatattg atggaaaatt tgtttttcaa accgaccgtg
     2101 gtaaggatag ggatggtatg cacacccgcc ctatgttccc aatactggaa acccaagttg
     2161 gtgatggctg ggttggtgat gttgattaca aacgcaacat gactaaacag gatagtgagt
     2221 acttggttga ttgggttcga atttatcaaa aagcagatca aagtcaggta cattttgata
     2281 atctagatga tgccaagcct aatgattatg ttattaagcc gcagcaaaaa cttggtcacc
     2341 tcgtaacagt ctctaacggg aaagcagctc atgaaaacaa aaacaatttc tactatggag
     2401 gtcaaccacg ttacgaaaca agcaggctct atgctgatgg tgatggcgaa aatgcactag
     2461 tttacaagat tgctaatcca gaggctttgc acttaacgac ttattacaaa acacttgaag
     2521 actattctgt ctataataaa gaagcagggg ctaatgaagg aaaatctgtt cgcaaacatt
     2581 tgataaatgc aaaagacggt gaaattgact tcactgtgta ttcatctgtt gacggcaaaa
     2641 gctggaataa agagaatgtt actgtagtgg ataattttgt agaagcaacc ccagcttatg
     2701 cacgcactac atttgatatt cgcaacattc gaaaaggtac taaatttgtg aagatcgtct
     2761 ttccaacggt caaaggtatt tcctataagt tggaagatgg aacagagaaa ggcctgcaag
     2821 ccagtgatgt ccagttagca aaagtaactt ttgtagctac tcatcctgaa gcagaaccag
     2881 aaaaacctat tgttgatcaa gaaaaactag ctttgtccaa agttttttca ctcgatgccg
     2941 gtcgcaagta tttctctgta aatcagataa aggagattat tgatttaatt tcttcttatg
     3001 gttacacgga tatgaacctc ttattgggaa atgatggtct gagattttta ttagatgata
     3061 tgaatttgac tgttggagat aaggtgtatg acagtgaagc agttagaaaa gcaataataa
     3121 atggtaataa gcattattat gacgatccaa acggtacaac cttaagtcag agtgagatgg
     3181 atgatattct tgcttatgct aaggaaaaaa atatttccat tattccaact atcaatagcc
     3241 caggtcatat ggatgcgatt gtagaagcaa tggaagaatt ggggataaaa tcgccaaaat
     3301 ttagttataa tggcaaaaca tctgcacgta cagttgattt aaaaaatgac caagcagttg
     3361 cttttactaa agccttaatt gataaatatg ctagttactt tgcaggcaaa tctaaaattt
     3421 tcaatattgg cttagatgag tatgctaacg atgctacaaa tgcttctgga tggcacattc
     3481 ttcagacaca aggtgattat ggtaagttta ttcaatatgc caatgattta gcagctatcg
     3541 tcaagaagca tggtttagag ccaatggctt tcaatgatgg tatctattat aaccacgata
     3601 ctagttcggg tacttttgat aaagacatta ttgcttcttt ctggacagga ggttggggag
     3661 gatatgatgt tgcttcctca aaatcccttc atgaaaaagg gcataaaatt cttaatacaa
     3721 atgatgcttg gtactatgtt attggacgtg aagcagaagg tttgggatgg tataaccttg
     3781 atcaaggtct tcgaggaact aaatcaacaa ggtttgatca agtaccgaaa tctgagaata
     3841 ctaaggtacc cattatcggt agcatggttg cggtatgggc agatgaccca agcaagcctt
     3901 acagctatga ctcaattaaa aaacttttgc acagctttac ggaaaacaat tctgattatt
     3961 ttttagcaga gtaccgagat ttgcgagaag aagttgggaa gattcctact gatttaaatt
     4021 cctatacgcc agagagcgtg gcgactttaa aagaggtctt gaacaagatt gattggaaac
     4081 taagtagaaa taaccaagcg tttgttgatg gttacttggc agaagtaaaa gcagcgcgtg
     4141 aagcgttagt tcctgtgaca gcaaaaggag acagcgtcaa agcagagtcc ttaccagaag
     4201 gcaagctacc tctgattact gctaaaggaa aaagtgtcca agcagaacca cgccctgcct
     4261 ttaagggata cctgacatca aactatggac catctgtcac acctttatct ctcgtatcaa
     4321 aaccaagcag tgagcagtca cgctcccagc aagcagcttc tcagaagaaa gaattgccaa
     4381 atacaggcgc agtagatggt cttggattct ctctccttgg tatgcttggt ctggcatttg
     4441 ctagtcgccg gcggaaggag gggtaatata gccatttgag tgaatgttca gggctttcta
     4501 attgtaatta catatcagtc atttagtttt tctttcactc gctttgccgt acctaagtat
     4561 agcaagcagc tcattgcctt gtacaaaaag taaactagaa ggctatgaat aacaggataa
     4621 ttttttatag gctactaaaa atcccccgta ataaatctta ttacgggaga ttttcgttta
     4681 tgag
//
LOCUS       DAZQCJ010000122         4565 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000122 DAZQCJ010000000
VERSION     DAZQCJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.124"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..925
                     /locus_tag="ACHU66_001927"
     CDS             <1..925
                     /locus_tag="ACHU66_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFR3390186.1"
                     /translation="NVVISSATLHFDITSDNNLIITVPLFGNKFLKLLKNLDEYLIPT
                     LGLPLISKQEFPDYVIFELGHIEKIEQYVFNSHTLTKEFFEDNPSPIIKLSNTQQFSL
                     KSNTNLGIYGRTGTGKTIALQWYLFNALAKGCGTTDNTYLGIVDGKGADLFALGKLLQ
                     EELGEQIAVGSSPQMLAKLSREFVQIMGERFEIIERNSTLNADAYDLGMTPNFLFVDE
                     LASININVELRNQISAILYLGNPGNDRLKIAFSMCELESVPTISDRKGEALFYADGIG
                     SVEPVITIVPFVDVKTKQQFLQVVRNILPNY"
     gene            1222..1641
                     /locus_tag="ACHU66_001928"
     CDS             1222..1641
                     /locus_tag="ACHU66_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390187.1"
                     /translation="MTYNMTDTTLKIGQLDLDMTQFEVPKKIVILSAKVNNKYNEVNG
                     EQVKTDEVVKVVCNVLDADKVKILKELGISTEDLKAINLEIVGNVDKIATLAQNKSLL
                     NVPIELIKPKVRLAWNMTRSNWAGVKLVCEDIKILGA"
     gene            1646..2662
                     /locus_tag="ACHU66_001929"
     CDS             1646..2662
                     /locus_tag="ACHU66_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFR3390188.1"
                     /translation="MNTVRIDYFAVTVKNIPPESILTDILLIPLEDFTLNNWGINKYQ
                     RHYACSEIKVYFNEDRPSMGVFIELKGQGCRQYEEFLDGNENNWIALIGRLYRYSVNF
                     TRLDIAHDIFDGSLDVQRVYDYCKKGLCISKAKHFEYHEKSVLENGERVGETVAIGSR
                     GNQQWCIYNKRMEQLSKQELVEYPSWIRAELRCWQDKANIIAEQLFLKRPLSSIYFEA
                     INGHYRFVKPNATDTNRWRRKKVKWWLDYLKTENQTVLSVERTKPTLRQSEAWTEKQV
                     AKTLAKVYTAKYQAYDVQKAEDYIQGLLKEGLSRLTDNDEKDIEQYIREQNSSPLWGQ
                     KKTT"
     gene            2723..2929
                     /locus_tag="ACHU66_001930"
     CDS             2723..2929
                     /locus_tag="ACHU66_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFR3390189.1"
                     /translation="MIATVNKNDLVALGFSEGTSKRIIRQGKELLIARGFRVYQNKRV
                     GTIPASIATELLGFDVSLGAHHDS"
     gene            2919..4052
                     /locus_tag="ACHU66_001931"
     CDS             2919..4052
                     /locus_tag="ACHU66_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR3390190.1"
                     /translation="MTVKKAKNGTWTVDISDGFHPVTKKRIRIIRKGFKTKKEALELE
                     QYIRVVELKEKRFDFLVTTDMLFDILEEEDRQNNRKISYISTQRNNYERHIKPYFINT
                     NLNKLSYEHIFEFREYLKIKPKKQNDSSTLSHNTVNKIMILLKKIFDTGVRKSLIDKN
                     PVENIRKLPISKPTINFWNVEEFNEFRKLITSDEISYDLFFTIAFFTGMRLGEIVALT
                     WYDINLITNSIHITKTAYFVNGTNHINSTKTRAGTRRITINHKLARMLADWKEKQKDL
                     LKEFTTDTENLQVIQSTPLPMTKNMIDKKFKQILARNDYLKKIRIHDLRHSHASLLIN
                     QGEDYLVVKERLGHASITTTIDTYSHLYPSKQKTLANKLDDIF"
     gene            complement(4139..4222)
                     /locus_tag="ACHU66_001932"
     tRNA            complement(4139..4222)
                     /locus_tag="ACHU66_001932"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4186..4188),aa:Leu,seq:caa)
     gene            complement(4234..4305)
                     /locus_tag="ACHU66_001933"
     tRNA            complement(4234..4305)
                     /locus_tag="ACHU66_001933"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4271..4273),aa:Gln,seq:ttg)
     gene            complement(4317..4389)
                     /locus_tag="ACHU66_001934"
     tRNA            complement(4317..4389)
                     /locus_tag="ACHU66_001934"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4354..4356),aa:His,seq:gtg)
     gene            complement(4397..4467)
                     /locus_tag="ACHU66_001935"
     tRNA            complement(4397..4467)
                     /locus_tag="ACHU66_001935"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4433..4435),aa:Trp,seq:cca)
     gene            complement(4475..4555)
                     /locus_tag="ACHU66_001936"
     tRNA            complement(4475..4555)
                     /locus_tag="ACHU66_001936"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4519..4521),aa:Tyr,seq:gta)
BASE COUNT         1602 a          769 c          911 g         1283 t
ORIGIN      
        1 caatgtagtt attagcagtg ctacattaca ttttgatatt acgtctgata ataatctaat
       61 tattacagtt ccactttttg gtaataaatt tctaaaactt cttaaaaacc tagacgagta
      121 tcttatacca acattaggac tgcctctaat atcaaaacaa gaattccctg attatgttat
      181 ttttgaactc ggtcacatag aaaaaattga acagtatgtt ttcaatagcc atacattgac
      241 taaagaattt tttgaagata atcctagtcc aataatcaag ctatcaaata ctcagcaatt
      301 tagtctaaaa tcaaatacta atctcgggat ttacggtcga actggaacag ggaagacaat
      361 agctctacaa tggtacttat ttaatgcact agctaaaggt tgtggcacta ctgacaatac
      421 ttatttggga attgttgatg gtaaaggagc tgatttattt gcccttggta aacttcttca
      481 agaagaatta ggggagcaaa tagcagtagg ctcttcgcct caaatgttag caaaattatc
      541 aagagaattt gttcagatta tgggggaacg ttttgaaatt attgagcgaa acagcacgct
      601 aaatgcagat gcttatgatt taggaatgac ccccaatttt ttatttgttg atgaacttgc
      661 ttccataaat ataaatgtag agctaagaaa tcaaatttcg gcaattttat accttggaaa
      721 tccgggtaat gaccgattaa aaatagcttt tagtatgtgt gaacttgaaa gcgtacctac
      781 tatatcagat agaaaaggag aagcgctttt ttatgctgac ggtataggtt cagttgaacc
      841 agtaataaca atcgttccgt tcgttgatgt taaaactaaa caacaatttt tacaggtagt
      901 tagaaatata ctgccaaact actaatggcg gtctttttcc ctagagaaat agggacgatt
      961 gcctagaggg caaaatcgag cggagcgttc ctcttttcgc tagggagaaa gagccaaccg
     1021 actgctttgc aggaggtcag aaaaataccc ccattactaa tagggggtgc acacatacat
     1081 gaaatgtact ctaaaccctt gatacgtaag gggttacggc ttgttggtta taccgaagtc
     1141 attgaaaccc ttgtgggaga agggcttgag tcgcttttcg tgtaggatac gactttttga
     1201 atagaaaatg gagtaattat tatgacttac aatatgacag atactaccct taagattggg
     1261 caacttgatt tggatatgac acagtttgaa gttccgaaaa aaattgttat cctatctgct
     1321 aaagtgaata acaagtataa tgaggttaat ggtgagcaag taaagactga tgaggttgtg
     1381 aaagttgttt gtaatgtact tgatgctgat aaggttaaaa tactaaaaga attgggaatt
     1441 tcaactgaag acttaaaagc tatcaactta gaaattgtag gtaatgtaga taagattgct
     1501 acacttgctc aaaataaatc gcttttgaat gttccgattg agttgattaa gcccaaagtt
     1561 cgtttagctt ggaacatgac aagaagtaac tgggcaggtg ttaaactggt atgtgaggac
     1621 attaaaattc taggagcata accctatgaa tacagtcaga attgattatt ttgcagtaac
     1681 cgttaaaaat attcctccag aaagtatttt aactgatatt cttttaattc ctttagaaga
     1741 tttcacatta aataattggg gaataaacaa gtaccagcga cattatgctt gttctgagat
     1801 taaagtgtac tttaatgagg atagaccttc aatgggggtt tttatcgaat taaaagggca
     1861 aggttgtcgc caatatgaag aatttttgga cggtaacgaa aataattgga tagctttgat
     1921 aggtcggttg tatcggtatt cggttaattt tactcgctta gatattgctc acgacatatt
     1981 tgacggtagt ttagatgttc agcgagtata tgactattgc aagaaaggtt tatgtatctc
     2041 gaaagcaaag cattttgagt atcatgagaa atctgtctta gaaaatggag aacgagttgg
     2101 agagacggtt gctataggtt ccagaggcaa tcaacaatgg tgtatctaca ataaacgtat
     2161 ggaacaactt agcaagcaag agcttgtaga atatccctca tggattcgtg cggaacttag
     2221 atgttggcag gataaggcga atattattgc agagcaattg tttctaaagc gtcctctgtc
     2281 atctatctat tttgaagcta tcaatggtca ctatcgtttt gtgaaaccaa atgcgacaga
     2341 tactaatcgt tggagaagaa aaaaggtcaa gtggtggcta gactatctaa agaccgaaaa
     2401 tcagactgtt ttgagcgttg agagaactaa gcctacatta cggcagtcag aggcttggac
     2461 agagaaacaa gttgcgaaga cattggcaaa agtctataca gcaaaatatc aagcctatga
     2521 tgttcaaaag gcagaggact acattcaagg tctactaaaa gagggcttgt caaggctaac
     2581 tgataatgat gaaaaggata tagagcaata tattcgagag cagaatagtt ctcccttatg
     2641 gggacaaaaa aagacgactt gaaaacaagt cgcctctaaa aattaactaa ctagattata
     2701 acaaaagaaa ggaaagtgtc caatgatagc aaccgtaaat aagaatgacc tagttgccct
     2761 tggtttctct gaaggaactt ctaaacgtat catcagacag gggaaagagc ttctaatagc
     2821 aagaggattt agggtttatc aaaataagcg tgtcggaact atccctgctt ctatcgctac
     2881 tgaattactt ggttttgacg tttctttagg tgctcatcat gacagttaag aaagcaaaaa
     2941 atggaacttg gactgttgat atttctgacg gcttccaccc agttaccaaa aagcggatac
     3001 gaatcattcg taaaggtttc aagacaaaga aagaagcttt agagttagaa caatatatcc
     3061 gtgttgttga gttaaaagaa aaacgttttg attttttggt aacaaccgat atgctcttcg
     3121 atatacttga ggaagaagac cgacaaaata acagaaaaat cagttatatc agcacacaaa
     3181 gaaacaacta tgagcgacat atcaaacctt acttcataaa tacaaactta aataaattat
     3241 cttatgagca catttttgaa tttcgagagt atctcaaaat aaagcccaaa aagcaaaacg
     3301 atagctctac gttaagtcat aatacagtca ataaaataat gatactcctc aaaaagattt
     3361 ttgatactgg tgtaagaaag tcattgatag ataaaaatcc agttgaaaat ataagaaaat
     3421 tgcctattag taagccaact ataaattttt ggaatgtaga agaatttaac gaatttagaa
     3481 aacttattac aagtgatgaa ataagttacg acctattctt tacgattgcc ttttttactg
     3541 gcatgcgatt gggcgagata gttgcgttaa cttggtacga tataaactta atcactaata
     3601 gcattcacat cacaaaaaca gcatattttg ttaacggtac aaatcatatc aatagtacaa
     3661 aaactagagc aggtaccaga cgtattacaa tcaatcataa attagcaaga atgcttgcag
     3721 actggaaaga aaaacaaaaa gacttactta aagaatttac aacagataca gaaaaccttc
     3781 aagtgattca aagcacacca ctaccaatga caaaaaatat gattgataaa aaatttaagc
     3841 agatactggc taggaacgac tatctaaaaa agatacgaat acacgattta aggcactctc
     3901 acgcgtcttt acttatcaat caaggggaag attatctagt cgtaaaagaa cgtcttggac
     3961 acgcctctat cacgactaca atagatacat attcccactt ataccctagt aaacaaaaga
     4021 cattggcgaa caaattggac gatatttttt agaaaatgga attttttggc aacttgaaaa
     4081 agagcaatag aaaagataaa acaaaagaca aggcttagaa accttgtctt tataactata
     4141 ccggcggccg gggtcgaacc ggcacgtcct tgcggacact ggattttgag tccagcgcgt
     4201 ctgccaattc cgccacgccg gcatatagtt gaactggggt agctggattc gaaccaacgc
     4261 atgagggagt caaagtccct tgccttaccg cttggcgata ccccaatatt aaaataggcg
     4321 agtgagggga atcgaacccc cgaatgtcag agccacaatc tgatgtgtta accacttcac
     4381 cacacccgcc atataacacg ggcagtagga attgaaccca cactgaaggt tttggagacc
     4441 ttagttctac ctttaaacta tgcccgtaaa ggtatggaga gagagggatt cgaacccccg
     4501 aacccgaagg agcggattta cagtccgccg cgtttagcct cttcgctatc tctccgaaat
     4561 ataat
//
LOCUS       DAZQCJ010000123         4477 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000123 DAZQCJ010000000
VERSION     DAZQCJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.125"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..697)
                     /locus_tag="ACHU66_001937"
     CDS             complement(<1..697)
                     /locus_tag="ACHU66_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HFR3390191.1"
                     /translation="MSFSVISSYLIQHPVLTALLIAHFLSDFTFQSQALADDKKLHLK
                     ALFMHLFIVSLPLLILALLTPVQNGDLFFQVWLSHLAIDYVKYFLNKHHWIKDSWEAG
                     AFLADQLLHVISIIILYHTIGVNVASHSLWIEPNYLLLQILFILLISKPVNILFKLYF
                     SKYQVAEGEEEQTVTGAGALIGQLECLIMGIFLLLGQYTAIGLVFTAKSIARYDKISK
                     SQAFAEYYLIGSLF"
     gene            complement(701..1369)
                     /locus_tag="ACHU66_001938"
     CDS             complement(701..1369)
                     /locus_tag="ACHU66_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HFR3390192.1"
                     /translation="MNYIAIIGDIVNSKQTSNRSSIQERLKQQLNRINHSFAQDFASP
                     FTITKGDEFQALCKPNPYIFLIIDQIQLAFRDEVEIRFGIGLGEILTAIDPKLSIGAD
                     GPAYWEARKAIDFIHDNHDYGTSKIAFSSEYKQIDRVINPLLTSSDFIKAAWNRSQTD
                     LFETLISKKIYQEDFTQKPIAEKMKLSQSAFTKRLKSSGIKLYLRNRQTAMNLILQLS
                     QKEG"
     gene            complement(1563..3137)
                     /gene="ffh"
                     /locus_tag="ACHU66_001939"
     CDS             complement(1563..3137)
                     /gene="ffh"
                     /locus_tag="ACHU66_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR3390193.1"
                     /translation="MAFESLTERLQNVFKNLRRKGKISESDIQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAIGHEVIETLNPAQQIIKIVDEELTAVLGSETSEIIKSPKIPT
                     IIMMAGLQGAGKTTFTGKLANKLKQEENARPLLIAADIYRPAAIDQLKTLGQQIDVPV
                     FELGNQVPALEIVRQGLEQAKANHNDYVLIDTAGRLQIDQALMAELREIKEFAQPNEI
                     LLVVDAMIGQEAANVAREFNDQLAITGVILTKIDGDTRGGAALSVRHITGQPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASKEYDEKKSLELAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMAGNPALANLKVDEREIARKRAIVSSMTPAERENPD
                     LLTPSRRRRIANGSGNSFVEVNKFIKDFNQAKTMMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMNGMDMSALEDMMGGAGMPDMSQLMGGAGMPDMSQMFGGGLKGRIGEFAMKQA
                     MKRQANKIKKAKKKRK"
     gene            complement(3150..3482)
                     /locus_tag="ACHU66_001940"
     CDS             complement(3150..3482)
                     /locus_tag="ACHU66_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFR3390194.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FQVSRQAVYDNIKRTEKLLEDYEMKLHMYSDYVVRSQIFDEILNKYPEDAYLKEKIAI
                     LTSIDNRE"
     gene            complement(3585..4283)
                     /locus_tag="ACHU66_001941"
     CDS             complement(3585..4283)
                     /locus_tag="ACHU66_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR3390195.1"
                     /translation="MKAAYITIHDKIKEQIDNGIWKIGQRLPSERDLADEFGVSRMTL
                     RQGITLLVEEGILQRKIGSGTYVASTRVQEKMRGTTSFTELVQLQGKTPSSKLLSYTR
                     TKPNEKEVEQLGLSRGEYVIRMERVRYADNVPVVYEVASIPERLIKNVPKEDVTNHFF
                     KTLMDNGYRIGTSKQTIFARLANEKVAQYLQISKNQAILALKQVSYLEDGQAFEFVNS
                     QYVGERFEFYLENN"
BASE COUNT         1269 a          897 c          867 g         1444 t
ORIGIN      
        1 taaatagcga accaattaaa tagtattcag caaaggcttg gcttttggat attttatcat
       61 agcgtgcgat ggattttgct gtaaatacca gcccgatagc tgtgtattgc cctagaagta
      121 gaaaaatccc cataatcaag cattctaatt ggccaatcaa ggctcctgca cctgtcacag
      181 tctgctcttc ttctccttcc gctacctgat atttgctaaa gtagagtttg aaaagaatat
      241 tgacgggctt gctgatcaga aggataaaaa ggatttgcag gaggagataa tttggttcaa
      301 tccagagtga gtgtgaagca acattgacac cgatagtgtg ataaagaatg atgatagaga
      361 tgacatgtag caattgatca gctaggaatg ctccagcttc ccaggagtcc tttatccaat
      421 ggtgtttgtt gagaaaatat ttgacatagt caatcgcaag atggctcagc caaacttgaa
      481 aaaataaatc tccgttttga acgggggtaa gtaaggctag aataagaaga gggagagaaa
      541 caatgaaaag gtgcatgaaa agagctttca aatgcagttt tttatcatcc gccagtgctt
      601 gactttggaa ggtaaaatca cttaaaaagt gggcaataag caaggcggtt aaaactggat
      661 gttgaataag atagctagaa ataactgaaa atgacatgct ttatccttct ttctgactga
      721 gctggagaat caaattcatg gcagtttgac gattacgcag gtagagtttg attccgctgg
      781 atttcaaacg tttggtaaag gcgctttgac tgagtttcat tttctcagca attggctttt
      841 gtgtgaaatc ttcttgatag atttttttgg agattaaggt ctcaaacaga tccgtttgcg
      901 aacgattcca ggcagctttg atgaaatcgc tagaagttag caaggggtta atcacacgat
      961 cgatttgctt gtattcagat gaaaatgcaa ttttgctggt gccatagtca tggttgtcat
     1021 gaatgaagtc aatagccttg cgtgcttccc aataagctgg accatctgct ccaatactta
     1081 gcttgggatc gatagctgtt agaatctcac ccaaaccaat tccaaatcga atctccacct
     1141 catcacggaa ggctagttgg atttggtcaa ttattaaaaa aatataaggg ttaggcttgc
     1201 ataaggcttg aaattcatct ccctttgtaa tggtaaatgg tgatgcgaag tcctgagcga
     1261 aggaatgatt gattctattc agctgctgtt ttagccgctc ttgaatacta gagcggttgg
     1321 aagtttgttt tgaattgaca atatctccta ttattgcgat ataattcata acttgtcctc
     1381 tgattcctat tatatttcca ttataacgga caatataaag attgtccact aaaaaggaca
     1441 tttttgaata tttccgttat aacggaaaat gttggggttc aaaaatcatt tttcctgctt
     1501 tgctacttct attttacatt gctgtatctt acttcttcga ataaaaagta aaaaagaaga
     1561 gattattttc tcttcttttt ggctttcttg attttattgg cttggcgttt catggcttgt
     1621 ttcatagcaa actcgccgat tctacctttc aaaccgcctc caaacatttg ggacatatct
     1681 ggcataccag ctcctcccat cagttggctc atatcaggca tgcctgcacc acccatcata
     1741 tcttccagag cagacatatc cataccgttc atgtttggca tgtttttagg gagattattt
     1801 gggttgattc ccatttgttt catcattttg ttcatgtcgc ctgacatgac accctgcatc
     1861 atggttttcg cttggttgaa atccttgata aatttattga cttcgacaaa gctatttcca
     1921 gaaccgttag caatacgacg acgacggctc ggtgtcaaca aatcaggatt ttctcgttcg
     1981 gctggtgtca tagaagatac gatagctcgt tttcttgcaa tttcacgctc gtcaactttc
     2041 aagttggcaa gtgctgggtt accagccata ccaggaatca tcttcagcag gtcttccatt
     2101 ggtcccatat tttgaacttg gtccagctga tcaatgaaat cgttgaaatc aaaggtattc
     2161 tcacgcattt tttcagcgag ttctagtgat ttcttctcat cgtattcttt gctggctttt
     2221 tcaatcaagg tcagcatgtc acccatgccc aaaatacgcg aggacatacg gtctgggtgg
     2281 aaggtttcaa tatctgtgat tttttcacca gtaccggtga atttgattgg ctgaccagta
     2341 atgtgacgga cagacaaggc tgcaccacca cgtgtatcac cgtcaatctt ggtcaaaatc
     2401 acaccagtaa tagccagttg atcattgaac tcacgcgcaa cgttggcagc ttcctgacca
     2461 atcatggcat cgacgaccaa gaggatttcg tttggctgag caaactcttt gatttctctc
     2521 aattcggcca taagggcttg gtcaatctgc aaacgaccgg ccgtatcaat cagaacgtag
     2581 tcattatgat tagctttggc ttgttctaaa ccttggcgta caatctccag ggcagggact
     2641 tgattgccca actcgaatac aggtacatca atttgttgac caagggtctt caactggtca
     2701 atggcagctg gacgataaat atcggcagca atcaagagtg gacgggcatt ttcttcctgc
     2761 ttgagtttat tagccaactt acctgtgaag gtcgttttac cagccccctg caatccagcc
     2821 atcataatga tagttggaat ttttggtgat ttgataattt ctgaagtttc agaacctaat
     2881 acagccgtca attcttcatc aacgattttg atgatttgtt gagcggggtt cagggtttca
     2941 atgacttcat gaccgatagc acgttcacgc accttcttga taaagtcttt tacaactggc
     3001 aaggcaacgt cggcttctag taaggccaga cgaatttcct tagttgcttc ctgaatatca
     3061 ctctcagaaa ttttcccctt acgacgcaga tttttaaaaa cattctgtaa acgttctgtt
     3121 aagctttcaa aagccatata cttctcctct tattctctat tatcaatact ggttagaata
     3181 gcgatttttt ccttcaaata agcatcttct ggatacttgt tcagtatttc atcgaaaatc
     3241 tggctacgca ctacatagtc agaatacata tgtagcttca tctcgtagtc ttccagcaat
     3301 ttttctgttc gtttaatgtt atcatagacc gcctgacggc tgacttgaaa ttcttcagca
     3361 atctcagcca aactgtaatc atcggcataa tagagctcga tgtagttcat ttgcttgtct
     3421 gttagcaagg ctgcatagaa ttcaaataag gcattcattc ggttggtttt ttcaatttcc
     3481 ataaacttta ttatatcaaa aattccatat tttataaata agaaaatccg tcatcactag
     3541 aaagattacg gattttaaaa atgttttaca taattatttt tatgttaatt gttttctaaa
     3601 tagaattcga aacgttcccc aacatactga ctatttacaa attcaaatgc ttgaccatcc
     3661 tcgagataag atacctgttt tagtgcgaga atagcctggt ttttagaaat ttgtaaatat
     3721 tgagccactt tttcgttggc taaacgcgca aaaatggttt gtttgctcgt tccaattcga
     3781 taaccattgt ccataagtgt tttaaagaaa tggttggtaa catcttcttt tgggacattt
     3841 tttatcaagc gttcaggaat gctggcaacc tcatacacga cgggaacatt atctgcatag
     3901 cggacccgct ccattcgaat aacatactcg ccacgggata aacctaattg ttcaacttct
     3961 ttttcattag gtttggttcg cgtataggac aataatttgc tgcttggcgt ctttccttgg
     4021 agttgtacta attcagtaaa ggaagtcgtg cctcgcatct tttcttgaac ccgtgtactg
     4081 gcgacataag taccggaacc aatcttgcgt tgtagaatac cttcttccac taaaagtgtg
     4141 attccttgac ggagcgtcat acgagaaaca ccaaattcat ctgctaaatc gcgttcactt
     4201 ggtaaacgtt gaccaatttt ccagattccg ttatcgattt gttccttaat tttatcgtga
     4261 attgtgatat atgctgcttt catttatcta gcctttctct tctttctagt ttaccaaaaa
     4321 taaaggcgtt tggctaattc aggccacact ctattggtaa aattctccta ggcaaacaat
     4381 gaagtcagat tacctaaaat aaagtgagta aaaacaataa aaatacgaac actaacattt
     4441 ttattatcac tcatagtttg caaaacagat atttaac
//
LOCUS       DAZQCJ010000124         4463 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000124 DAZQCJ010000000
VERSION     DAZQCJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.126"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            22..1572
                     /gene="guaA"
                     /locus_tag="ACHU66_001942"
     CDS             22..1572
                     /gene="guaA"
                     /locus_tag="ACHU66_001942"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR3390196.1"
                     /translation="MTKQDVQKIIVLDYGSQYNQLISRRIREFGVFSELKNHKITAEE
                     VRAINPIGIVLSGGPNSVYAENAFDIDPEIFELGIPILGICYGMQLITHKLGGKVVPA
                     GEAGNREYGQSNLQLKTESALFAGTPEEQLVLMSHGDAVTEIPADFHLVGLSADCPYA
                     AIENTERRIYGIQFHPEVRHSVYGNDILKNFAFGICGAKGDWTMENFIETEIEKIRQT
                     VGDKKVLLGLSGGVDSSVVGVLLQRAIGDQLTCIFVDHGLLRKNEGDQVMEMLGGKFG
                     LNIIRVDAAKRFLDLLAGVSDPEKKRKIIGNEFVYVFDDEASKLTDVEFLAQGTLYTD
                     IIESGTDTAETIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRALGTALGMPDEVVWRQ
                     PFPGPGLAIRVMGEITEEKLQTVRESDAILREEIAKAGLDRDVWQYFTVNTGVRSVGV
                     MGDGRTYDYTIAIRAITSVDGMTADFAKLPWDVLQKISVRIVNEVDHVNRIVYDITSK
                     PPATVEWE"
     gene            complement(1734..2783)
                     /gene="mcrC"
                     /locus_tag="ACHU66_001943"
     CDS             complement(1734..2783)
                     /gene="mcrC"
                     /locus_tag="ACHU66_001943"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methylcytosine-specific restriction
                     endonuclease system specificity protein McrC"
                     /protein_id="HFR3390197.1"
                     /translation="MIPVQNIYYMLSYAFKILHKQEYKRIATEEFKNAADLLAEIMII
                     SLSIQVKRGLGREYRSQTESLSALKGKINISESLTPPNWRRKQLVCQYDDFSLDSRMN
                     RIIKASIEILLKADISRDRKKKLRKLLVFFGEVSKINLHSINWNLQYNRNNQSYQLLM
                     SICYLVVNGLIHTEREGNKKLMNFLDERRESLLYEKFILGYYKKHYPQIQVTASQIPW
                     ALDDGFGEMLPIMQSDIYLKYKDTILIIDAKYYSSNTQIRFDKRTLHSNNLYQIFTYV
                     KNQAYRLSDSNDTVAGMLLYAKTDIDIQPNQVYQMHGNQISVKNLDLNLQFASIAEQL
                     DDIITSHFVSPPKRY"
     gene            complement(2780..>4463)
                     /locus_tag="ACHU66_001944"
     CDS             complement(2780..>4463)
                     /locus_tag="ACHU66_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR3390198.1"
                     /translation="QFNWVNFYKEFAWKLLDYKDNRAELVEKVKAIYTKTKIHMPTLE
                     KDNHLIDIDPFTIFGLFNKQIKEENRIKILTAIAELFNLQTEIPSSFESIPVLMNQNA
                     TFYYFIGDRDEKDIDDLWELFIAALTYSKEPTAEKKEKFSHYFDLAINKKGNGNSKIT
                     MALYWIAPDFFLNLDSRNKWYVYESGEIPTDVIKKLPKMKSKISADDYFYISDSMHHY
                     LNSAESKLKDFKELSSMAWKYSKQINQEEKERSAGAISPLADETDTGNEIGSEEVVED
                     SYTKNHFLSEVYMTEEEYDQLTSILQMKKNIILQGAPGVGKTFVAERLAFSLMGKPDL
                     ERVMTIQFHQSYSYEDFIMGFRPSTSGFELRRGAFYTFCKKAESDKSHDYFFIIDEIN
                     RGNLSKIFGELFMLIENDKRGKSLQLLYSDEQFSIPENLYIIGMMNTADRSLALLDYA
                     LRRRFAFFDIKPGFSTAGFKDYQTNLKNEKFDRLISCIEKLNQAISTEEVLGDSFCIG
                     HSYFCGLSAEVLDQGLLEAIVEYELIPLLKEYWFDEPTKIHDWSSKLRSAIQ"
BASE COUNT         1268 a          880 c          867 g         1448 t
ORIGIN      
        1 ttcaaaacga aagggaaaaa catgacaaaa caagatgtcc aaaaaattat tgtattggat
       61 tatggtagcc agtacaacca acttatttca cgtcgaattc gtgaatttgg tgtcttttca
      121 gaattgaaaa accataaaat tacagctgag gaagttcgtg ctatcaatcc aattggtatc
      181 gttctttcag gtggaccaaa ctctgtctac gcagaaaatg cctttgatat tgatccagaa
      241 atctttgaat tgggtattcc tatcttaggg atttgctatg gtatgcagtt aattacccat
      301 aaattgggag gtaaggttgt tcctgctggt gaggctggaa atcgtgaata tggtcaatca
      361 aatcttcaac tgaagactga atcagcgctt tttgctggaa caccagaaga acagcttgta
      421 ctcatgagcc acggggatgc tgtaactgaa attcctgctg atttccacct ggttggtctg
      481 tctgctgact gcccttacgc tgccattgaa aacacggaac gccgtatcta cggtatccag
      541 ttccacccag aagttcgtca ttccgtatat ggaaacgata ttttgaaaaa ctttgcgttt
      601 ggtatctgtg gagccaaagg cgactggaca atggaaaact tcatcgaaac agaaattgaa
      661 aaaatccgcc agactgttgg ggacaagaaa gtcttgctcg gtttatcagg cggtgtggat
      721 tcatctgttg ttggtgtgct tcttcaacgt gctattggtg accaattgac ctgtatcttc
      781 gttgaccacg gtcttcttcg taagaatgaa ggcgatcaag ttatggaaat gttgggcggt
      841 aaatttggcc tcaatattat tcgcgtcgat gctgctaagc gtttcttgga tctacttgct
      901 ggcgtatctg accctgagaa gaaacgtaaa atcatcggta atgaatttgt ttacgtattt
      961 gacgatgaag cgagcaagtt gactgatgtt gaattcctag cgcaaggaac actttatacc
     1021 gatattatcg agtcaggaac ggatactgct gagacaatca aatctcacca caacgttggt
     1081 ggtctgccag aagatatgca gttcaagttg attgaaccgc tcaatactct ctttaaggat
     1141 gaagttcgtg ctctcggtac tgctcttggt atgccggatg aagttgtatg gcgccaacca
     1201 ttcccaggac caggccttgc cattcgtgtc atgggtgaaa tcactgagga aaaacttcaa
     1261 actgttcgcg aatctgacgc tatcttgcgt gaggaaattg caaaagcagg tcttgaccgc
     1321 gatgtgtggc aatatttcac ggttaatact ggcgttcgct ccgtaggtgt catgggagat
     1381 ggtcgtacct acgactacac catcgccatc cgtgctatca cttctgtcga tggaatgaca
     1441 gctgactttg ccaaactacc atgggatgtt cttcaaaaaa tctctgtccg tatcgtaaac
     1501 gaagtcgacc acgtcaaccg catcgtctat gatataacaa gtaaaccacc agcaacagtt
     1561 gagtgggagt aaattagtcc agtggactaa tttacccgat gcctaaaaat aaaaaagcat
     1621 cgctgatctg ggaaatcaat taaccagacg ctccaaaata taaaaaaaca atcaaagagt
     1681 agtttagtgc tactactttg attgtttttt tgttttctag cttaaagctc taactagtac
     1741 cgttttggag gggatacaaa atgcgatgta atgatgtcat ccagttgttc agcaatactt
     1801 gcaaactgca ggtttaagtc taaatttttg acactaatct ggtttccatg catttggtag
     1861 acctggttag gttgaatatc tatatcggtt ttagcgtata gaagcattcc tgctactgta
     1921 tcgtttgaat ctgacaggcg ataagcctgg tttttcacat aggtaaaaat ttggtataga
     1981 ttatttgaat ggagtgtgcg cttatcaaag cgaatctgtg tgttactaga ataatattta
     2041 gcatcaataa tcaatatagt atctttatat ttgaggtaaa tatcactctg catgatgggg
     2101 agcatttctc caaaaccatc atctagtgcc caaggtatct gagaggcggt aacctgtatt
     2161 tggggataat gctttttata atatccaaga atgaacttct catatagcaa ggattccctc
     2221 cgctcatcaa gaaaattcat caatttctta ttcccctccc tctctgtatg aattaatcca
     2281 ttcaccacca aatagcagat tgacattagt aattgatacg actgattgtt tcgattatat
     2341 tgtaaattcc aattgattga gtgcagatta atttttgaga cttctccaaa gaaaactaag
     2401 agttttctca atttcttttt cctgtccctg cttatatcag cttttagcag tatctcaatg
     2461 cttgctttga taatgcgatt cattctactg tcaagagaaa aatcatcgta ttggcaaaca
     2521 agttgctttc tacgccaatt cggaggtgtt aaagattcag agatatttat tttaccttta
     2581 agtgccgaga gtgactcggt ttgagaacga tactctcgac caagtcctct tttcacctgt
     2641 atcgagaggc tgattatcat tatttccgcc agtaaatctg cagcattttt aaattcttca
     2701 gtagcgatcc gtttatattc ctgcttatga aggattttga atgcgtagga tagcatatag
     2761 tagatatttt ggacaggtat cactggatgg cactccttaa cttactactc caatcatgaa
     2821 tttttgtcgg ttcatcaaac caatattctt tcaagagggg aatcagttca tattcaacaa
     2881 tggcttctag caaaccttgg tccagtactt ctgccgataa accgcagaag taactatgcc
     2941 caatacagaa gctatctccc agtacttcct ctgttgaaat ggcctgattt agcttttcta
     3001 tacaggaaat gagtctatcg aatttttcgt ttttcagatt tgtttgataa tctttgaatc
     3061 ccgcagttga aaaacctggc ttgatgtcaa agaaggcaaa gcgtcgtctc agtgcataat
     3121 caagtagggc taaactccta tctgcggtat tcatcatgcc gatgatatat agattttctg
     3181 gaattgagaa ttgctcatcc gagtagagga gctgtaaaga cttgcctcgt ttgtcatttt
     3241 ctatcagcat aaacaattca ccaaaaattt tgctcaaatt tcccctgttt atttcatcga
     3301 taataaagaa ataatcatga ctcttgtcag actctgcttt tttgcagaaa gtatagaatg
     3361 cccctcttct cagctcaaag ccacttgtcg aaggtctaaa gcccatgatg aagtcctcgt
     3421 aggaatagct ttggtgaaac tgaattgtca tgaccctttc taaatctggt ttacccatta
     3481 atgaaaatgc taggcgttct gcaacaaacg tctttccaac tcctggcgct ccttgtaaga
     3541 tgatattctt tttcatttgg agtatggagg ttaattgatc atactcttct tctgtcatat
     3601 agacttctga taggaagtga tttttcgtat agctatcttc tactacctct tcactaccta
     3661 tctcgtttcc agtatcagtt tcatcagcca gtggagaaat tgctccagca cttctttcct
     3721 tctcttcttg attgatctgt ttagagtatt tccaagccat ggatgataat tctttaaaat
     3781 ctttcagttt gctctctgca ctgtttaaat aatgatgcat agagtccgag atataaaaat
     3841 aatcatcggc agaaattttt gacttcatct ttggtagttt tttgatgaca tcagtaggaa
     3901 tctccccaga ttcgtataca taccacttat ttctgctatc taagttcaag aaaaagtcag
     3961 gagcaatcca atacaaggcc atcgtaatct tgctgttgcc atttcctttc ttgtttatag
     4021 ccaaatcaaa atagtgtgaa aatttttctt tcttctcagc cgtaggctcc ttgctatagg
     4081 tcaaagcagc aatgaaaagt tcccataagt catcaatatc tttctcatca cgatctccga
     4141 taaaataata gaaagtcgca ttctgattca tcagtactgg tatactttca aaagaggatg
     4201 ggatttccgt ttggaggttg aataattctg ctatagctgt cagaatttta atgcgattct
     4261 cctcttttat ttgcttgttg aagagtccaa atatcgtaaa aggatcaata tctatcaggt
     4321 gattgtcctt ttctaacgtg ggcatatgta tctttgtttt ggtatagata gcttttactt
     4381 tctcaaccag ctctgctcta ttatctttat agtctagtaa tttccaagca aattccttat
     4441 aaaagtttac ccaattgaat tga
//
LOCUS       DAZQCJ010000125         4217 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000125 DAZQCJ010000000
VERSION     DAZQCJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.127"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(32..727)
                     /locus_tag="ACHU66_001945"
     CDS             complement(32..727)
                     /locus_tag="ACHU66_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFR3390199.1"
                     /translation="MISNEQYDYLRAHPTFERLTRDSFDQLAKHIRFRKIPKGQIFFY
                     AEDPRDYLFVLYKGYARIEQYDETDSFTYLDYIRQGGAFPFGDMFQDKPYHYTAIAIT
                     DLEYFIVPMVLFETLSKINPSQMVYICQKLSKVLRFQELRLRNAMRSKASDRVVQALA
                     LLYWDMCQRDQFATLPFEIHIQEISRLAATTRETVSHVLKQLKQDGKIAYSHKQLTYL
                     DIDYFLENLTETH"
     gene            complement(1011..1928)
                     /gene="whiA"
                     /locus_tag="ACHU66_001946"
     CDS             complement(1011..1928)
                     /gene="whiA"
                     /locus_tag="ACHU66_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFR3390200.1"
                     /translation="MSFTVQVKEELLLQSSQNKSELSAMIKLSGSLGLASSGLTLSIS
                     TENAKIARHIYELLLHFYQIKAEIRHHQKPNLKKNRVYAVLIEDGVNEILNDLHLADS
                     FFGLETGISPLVLENDSWSQAYLRGAFLAAGSVKDPEKGKYQLEIASVYSDHANDLAN
                     LMQKFLLDAKVIERSKGTITYLQRAEDIMDFLLVIGAEETKTEFENVKLLREARNDLN
                     RATNAEAANIAKTVNASMKTINNIIKIMDTIGLDQLSGDLQEIAQLRIQHPDYSIQQL
                     ADSLTVPITKSGVNHRLRKINKIADELTE"
     gene            complement(1925..2899)
                     /locus_tag="ACHU66_001947"
     CDS             complement(1925..2899)
                     /locus_tag="ACHU66_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HFR3390201.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLRDKDVEITAIVTVADDGGSSGE
                     IRQALQVTPPGDLRNVLLAMSDMPKLYEQIFQYRFADSDGPLAGHPLGNLIIAGISEM
                     QGSTYNAMRLLTRFFHTTGRIYPSSEQALTLHAIFTDGTEVAGESKISKHNGMIDHVY
                     VTNSYNDDEPKASRQVVETIMESDMIVLGPGSLFTSILPNLMISDIGKALKETKAEVT
                     YVCNIMTQRGETEFFSDADHVAVLNAHLAEQFIDTVLVNIEPVPQEYMNSNQFDEYLV
                     QVKHDFAGLQKQANRVISSNFLRLENGGAFHDGDLVVEELLKILQVRP"
     gene            complement(2896..3783)
                     /gene="rapZ"
                     /locus_tag="ACHU66_001948"
     CDS             complement(2896..3783)
                     /gene="rapZ"
                     /locus_tag="ACHU66_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR3390202.1"
                     /translation="MSDKLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPTLLPKF
                     LELIRHSQDNNKIALVVDMRSRSFFSEIREVLDEIEGAEDLDFKVLFLDATDNELVAR
                     YKETRRSHPLAADGRVLDGIQLERELLAPLKNMSQNIIDTTELTPRNLRKAISEQFAS
                     QDNQPSFRIEVMSFGFKYGLPLDADLVFDVRFLPNPYYKLELRNLTGLDAPVFDYVME
                     HQESEEFYSHLLGLIEPILPGYQKEGKSILTIAVGCTGGQHRSVAFAKRLADDLEKNW
                     NVNRSHRDKDRRKETVNRS"
     gene            complement(3805..4182)
                     /locus_tag="ACHU66_001949"
     CDS             complement(3805..4182)
                     /locus_tag="ACHU66_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR3390203.1"
                     /translation="MKTIHTDKAPAAIGPYVQGKVVGNFLFASGQVPLSPETGEVVGE
                     TIQEQTEQVLKNIAAILSEAGTDFDHVVKTTCFLKDMNDFVAFNKVYKTAFSADFPAR
                     SAVEVARLPRDVKVEIEVIAVID"
BASE COUNT         1273 a          983 c          745 g         1216 t
ORIGIN      
        1 tttttatttt tttataaaaa aaacagaaac ttcaatgagt ttctgtcaaa ttttctaaga
       61 aataatcaat atctaaataa gttaactgtt tatggctata agcaatttta ccatcctgct
      121 tcagttgctt caaaacatgg ctaaccgttt cccgtgtcgt agctgctaaa cgcgaaatct
      181 cttgaatatg aatttcaaat ggcaaggtag caaactgatc gcgctggcac atatcccaat
      241 aaagaagagc caaggcttga acgactcgat ctgatgcctt ggaacgcatc gcatttcgca
      301 agcgcaactc ttgaaaacga agcactttgg ataatttttg acagatataa accatttgac
      361 tcgggtttat cttcgacaag gtttcaaaca agaccatcgg tacaataaaa tactctaggt
      421 ccgttatggc aatggcggta taatgataag gtttgtcctg aaacatatct ccaaaaggaa
      481 aggcaccacc ctgccgaata tagtccaaat acgtaaaact atccgtctca tcatattgct
      541 caatccgagc ataaccctta tacaatacaa atagataatc tctaggatcc tctgcgtaaa
      601 aaaagatttg ccccttagga atctttcgaa aacgaatatg cttagctaat tgatcaaagc
      661 tatcacgtgt caaacgttca aaagttggat gtgcccttag ataatcatat tgttcgttgc
      721 ttatcatcaa aactcctttc tagctaaagt atccttctat ctattttaac atatattaac
      781 gttctatgct aatcattact aaaaaattca taaaacagaa tcattccagt gaagaagatt
      841 gctatcacag catacaaaaa actggtcctc tcgaaccagt cttagtatcg tcgaatgaat
      901 taactttcag acaaggaact gaggtgcaag caatactaga gtacggcaag ccgagagtga
      961 cgatgtatca aagttagttc aattgtccat aacattctat gtatacctat ctattcagtc
     1021 aattcatccg caatcttatt gatttttcgc aaacgatggt tgacaccact tttagtaatc
     1081 ggtaccgtta gactatccgc caattgctga atggaatagt ctggatgctg aattcgcaac
     1141 tgggcaatct cctgcaagtc acctgacaac tgatctagac cgatagtatc cataattttg
     1201 ataatattat tgattgtttt catactagca ttgaccgtct ttgcaatatt agcggcttcc
     1261 gcatttgtag ctcgatttag gtcattgcga gcctcacgaa gcaatttaac attctcaaat
     1321 tctgtctttg tctcttctgc tccaatcacc aaaagaaaat ccataatgtc ctctgcacgt
     1381 tgcagatagg taatggttcc cttacttcgc tcaatgactt tggcatccaa aagaaatttc
     1441 tgcatcagat tggccaggtc attggcatgg tcactataga cagaagctat ttcaagctgg
     1501 tattttcctt tttcaggatc cttaacagag cctgccgcta aaaatgctcc acgaagatag
     1561 gcttgactcc acgaatcatt ttccaaaacc aagggagaaa tccccgtctc taatccaaag
     1621 aagctatcag ccaaatgcaa atcgttcaat atctcattca cgccgtcttc tatcaaaact
     1681 gcatacacac gattcttttt gagattaggt ttttgatgat ggcgaatctc agccttaatc
     1741 tggtagaaat ggagtaacag ttcataaatg tgacgggcaa tctttgcatt ttccgtgctg
     1801 atagaaagag tcaaacctga tgaagctaag cccaaactac ctgataattt gatcatagca
     1861 gatagctcgc tcttattttg gctagactga agcagcaatt cttcctttac ttgtactgta
     1921 aaactcatgg ccgcacctgc aaaatcttga gcaactcttc taccaccaag tcaccatcat
     1981 ggaaagctcc gccattttcc aaacgaagaa aattcgaaga aatcacacga ttagcctgtt
     2041 tttgtaaacc tgcaaaatcg tgttttacct gtaccaagta ttcatcaaac tggttgctat
     2101 tcatatactc ttgtggaact ggctcaatat tgaccaaaac tgtatcaatg aattgttcag
     2161 ctaggtgagc attgagaacc gccacgtggt ccgcatctga gaaaaactca gtctctcccc
     2221 gttgggtcat aatgttgcac acataggtca cttcagcctt ggtttctttt agggctttgc
     2281 caatgtcaga aatcatcaga ttcggcaaga ttgaggtaaa aagagaaccc ggtccaagta
     2341 caatcatgtc actttccata atcgtttcaa ctacctggcg actagctttc ggttcatcat
     2401 cgttataaga atttgtcaca tagacatgat caatcatccc attatgcttg gaaatcttgc
     2461 tctcacctgc tacctcggtc ccatctgtaa aaatagcatg aagggtcaag gcctgttcgc
     2521 tagatggata aatccgccct gtcgtatgga aaaagcgtgt caacaacctc atcgcattgt
     2581 aggttgaacc ctgcatttct gaaataccag caatgatgag attccctagt ggatgaccag
     2641 ccaagggtcc atctgaatcc gcaaaacgat actggaaaat ttgttcataa agtttgggca
     2701 tatccgacat agccaaaagg acgttacgca aatcacctgg aggggtcacc tgcaaagcct
     2761 gacgaatttc ccccgaagat cctccgtcat cagctaccgt cacgatagcg gtaatctcta
     2821 catccttatc ccgcaaactc ttcaaaatga ctggaatacc agttccgccc ccgataactg
     2881 taatttttgg ttttctcatg agcggttgac cgtctccttc cgtctgtcct tatcacgatg
     2941 actacgatta acattccaat ttttctctaa atcgtctgcc aaacgtttag caaaggctac
     3001 gcttctatgc tgaccacccg tacatccaac agcaatcgtt agaattgatt ttccttcctt
     3061 ctgataacca ggaagaatcg gttctatcaa gccgagcaaa tgactataaa actcttccga
     3121 ttcttgatgc tccatgacat agtcaaagac aggagcatct agaccggtca gattacgcaa
     3181 ctccaacttg tagtagggat ttggtaagaa ccgaacgtca aaaaccaagt ctgcatccag
     3241 aggtagtcca tatttgaaac caaaggacat cacttctatg cgaaacgacg gctgattatc
     3301 ttgactagca aactgctccg agattgcctt acggagatta cgaggagtca gctctgtcgt
     3361 gtcaataata ttttgactca tatttttcaa gggagccaaa agttcacgct ctagctgaat
     3421 cccgtccaaa acacgaccat cagccgccaa aggatgggaa cgacgagttt ctttatagcg
     3481 agcaaccaat tcattatccg tcgcatccaa gaataggact ttaaaatcta aatcctctgc
     3541 cccctcaatc tcatccaaca cctctcgaat ctccgagaag aaggagcgac tccgcatatc
     3601 tacaacaaga gcaatcttat tgttatcctg gctgtggcga atcaattcta aaaattttgg
     3661 caagagagtc ggtggcatat tatcaatggt aaaatagccc aagtcctcaa aagactgaat
     3721 ggctaccgtc ttaccagctc ccgacatccc tgtcacaatt actaaatgga gtttgtccga
     3781 catgacattc cctccttttc tcttctaatc aataactgca ataacttcaa tctcaacctt
     3841 aacatcacgt ggcaaacgag caacttccac cgctgaacga gctggaaaat ctgctgagaa
     3901 agctgtttta taaactttat taaaggctac aaaatcattc atatctttta ggaaacaagt
     3961 cgtcttcacc acatggtcga agtctgttcc tgcttctgat aaaattgctg cgatattttt
     4021 caagacttgc tcagtctgct cctgaatggt ttcaccaacc acttcaccag tttcaggtga
     4081 caaaggaact tgaccagagg caaataggaa atttccaaca accttccctt gaacgtatgg
     4141 gccaattgct gcaggtgcct tatctgtgtg aatggttttc atatctctat ctccttttaa
     4201 ttttttatat ctattat
//
LOCUS       DAZQCJ010000126         4199 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000126 DAZQCJ010000000
VERSION     DAZQCJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.128"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..545)
                     /locus_tag="ACHU66_001950"
     CDS             complement(<1..545)
                     /locus_tag="ACHU66_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR3390204.1"
                     /translation="MNRLKDLRTKHKLTQQQTADIAGVTRRGYQKWESGESSIKADKA
                     QILADYFGVPVAYLLGYHEETISFTFPNDDEGFYKETQKLIDIADNIDPNSEIYSQVQ
                     QVKDMAILARSSREKIKARYGQKVYAGLNDKETDFEFYQYLGFLLNKEEYLKSDLLVY
                     LSLLDSSDKEIVFKMITEFKK"
     gene            703..891
                     /locus_tag="ACHU66_001951"
     CDS             703..891
                     /locus_tag="ACHU66_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR3390205.1"
                     /translation="MIITETQAKAIRRKIADKQLKQYEFAKEIGVASRTVPKIVAGNY
                     KAPKRIYAAVMEWLAKDY"
     gene            968..1345
                     /locus_tag="ACHU66_001952"
     CDS             968..1345
                     /locus_tag="ACHU66_001952"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390206.1"
                     /translation="MARGFVRYLVRVKTIALIKYIYYKFTKNKGENQKMALSTRNIKQ
                     QGNQIAELLSRIEIIQQLGNALLLADNAGADSATLHYQMKQAFSVIFEMTEQLYKDLD
                     LIACKLINCDDDKELEVIRQHER"
     gene            1335..1538
                     /locus_tag="ACHU66_001953"
     CDS             1335..1538
                     /locus_tag="ACHU66_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390207.1"
                     /translation="MNDKILSNYELLCTELENVISTLEIASGDIEPGKATALVTVATN
                     GLKHLVSEHTELSDKFFKEYGNE"
     gene            1531..1734
                     /locus_tag="ACHU66_001954"
     CDS             1531..1734
                     /locus_tag="ACHU66_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390208.1"
                     /translation="MNELIPTLTESVILIIVSLAILILLWRYESYIELDISPQTDKVE
                     ENTTAHVQERYGAYVWLAGKKFN"
     gene            1745..2011
                     /locus_tag="ACHU66_001955"
     CDS             1745..2011
                     /locus_tag="ACHU66_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390209.1"
                     /translation="MSTFSVEFEQGLLDRVDKLAQQKLELEKKLQKKTGLITAKELKD
                     DLDISGTTLKNWMDIGLVSYQSPFESSKKLYFKVSDVINFLTVR"
     gene            2022..2180
                     /locus_tag="ACHU66_001956"
     CDS             2022..2180
                     /locus_tag="ACHU66_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390210.1"
                     /translation="MKVFIIDGDAWRGKAHYSPSLDVVFISDKVDKHARDELIERVTK
                     SSNRTIWR"
     gene            2185..2400
                     /locus_tag="ACHU66_001957"
     CDS             2185..2400
                     /locus_tag="ACHU66_001957"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390211.1"
                     /translation="MAKEHYEVTSVFPAGWIVNGKKLTEPIEMSAEELRKSEVYPPEN
                     SSVYDIFKKINEERWEETRKDGNKAND"
     gene            2378..3208
                     /locus_tag="ACHU66_001958"
     CDS             2378..3208
                     /locus_tag="ACHU66_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HFR3390212.1"
                     /translation="MATRRMISKEVVMTDNFLDLPPTTKVLYFFLNLEADDDGFVGNP
                     KTVMRLTETTKDDMKLLIDSEYVLLFDTGVVVITDWTEHNAIRKDRKKDTRFTKEMQQ
                     IILVDGGKYFWLSDSQPNDNQSATINTPNGCVGEDRRGKDSIGKERVVEVDKEQNQSP
                     TPPPFNQDFVNLYKSFEAETGKALSPIQMQELQYMLEDFSADVIHEALREAVGQGKAN
                     FAYIQAILKRWKQDNLLTVELIRNNKAAREVKKQQNNSKPEPQTREEWLANWSEENPF
                     "
     gene            3224..4069
                     /locus_tag="ACHU66_001959"
     CDS             3224..4069
                     /locus_tag="ACHU66_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR3390213.1"
                     /translation="MLTQAEFIANTKTLEETCPIHGIPLMQLDRVVKIAEEDKPRKLS
                     PICPECIKEQNEKQVQEEVEKHLNAGIYQKTYNVLMRDSTIPEDLKAASFDNFIIETP
                     EEKQMLDFVKNQTQKYLDGMNGNTLLTGTTGVGKTHLAISLAKELNEAYRAKGEPKSV
                     LFINLTEILREIRESFKFTSKEGYYSRMLKEVDYLILDDLGVKLGNASGQSKSAWEEE
                     FIFDVLSHRNNTIITTNLSNDEIANLYSERVASRVRTGLDGNYFKVFSIKDKRYSINK
                     LKSNM"
BASE COUNT         1490 a          671 c          852 g         1186 t
ORIGIN      
        1 tattttttaa attcggtaat cattttaaaa acaatctctt tatccgatga atcgagtaat
       61 gaaagataca ccagcaaatc acttttgaga tattcttctt tatttaatag aaagcctaga
      121 tactggtaaa attcaaaatc tgtttcttta tcatttagcc cagcatatac tttttgacca
      181 tatcgagctt ttattttttc acgactactc cgtgctagta tagccatgtc tttaacttgc
      241 tgcacctggg aataaatttc actatttggg tcaatattgt cagctatatc aattagcttt
      301 tgagtctctt tatagaaacc ctcgtcatca tttggaaaag taaaactgat agtttcttca
      361 tgatagccga gtagataagc aacggggacg ccgaaataat cagctaatat ttgtgctttg
      421 tcagctttta ttgagctttc accactttcc catttttggt aacctcgtct agttacacca
      481 gcaatatcag ccgtttgttg ttgggtaagt ttgtgcttag tacgtaaatc ttttaatcta
      541 ttcacttttt ttcacctcat agcgattata acacaatttc acgagataat gctaactttt
      601 tttcactttt ttatcataaa cgcttgacaa gggaacgaaa gttagctaaa atgctatttg
      661 taatatattt gctaactaaa gttagctaga aaagaggaaa ttatgattat tacagagaca
      721 caagccaagg ctattcgtcg aaagattgct gataagcagc taaagcaata tgaatttgct
      781 aaagaaatag gtgtggctag tcgtacagtt cctaaaatag tagctggtaa ctataaagca
      841 ccaaagcgaa tttatgcagc agtcatggaa tggttagcaa aagattatta aggtacaaaa
      901 aagccatgta caggcgacca aaccaacgta cacggctaag gaaaaataac aaaactcaag
      961 caaaggcatg gcgcgtggtt ttgttaggta tttagtaaga gtgaaaacta tcgcccttat
     1021 aaaatatatt tactataagt ttaccaaaaa caaaggagaa aatcaaaaaa tggcattaag
     1081 cacacgaaac attaagcaac agggtaacca gattgctgag ttgctctcac gtattgaaat
     1141 tatccaacaa ctaggcaatg ctttattact tgctgataat gctggagcag atagcgctac
     1201 tttacactat caaatgaaac aagcatttag cgttattttt gaaatgactg aacagctata
     1261 taaagactta gatttaatcg cttgtaagtt gataaattgt gatgatgata aagaattgga
     1321 ggttattaga cagcatgaac gataagattt taagtaacta tgaattgcta tgtactgagt
     1381 tagaaaatgt tatcagtact ttagagatag ctagcggcga tattgaacca ggcaaggcaa
     1441 cagcccttgt aacagtagca accaatgggc taaagcatct tgtttcagaa cacacagaat
     1501 tatcagataa atttttcaag gagtatggca atgaatgaat taatcccaac attgactgaa
     1561 agtgttatct tgattattgt ttccttggct attctaattt tattatggcg ttatgagagc
     1621 tatatagagc ttgatattag cccacaaact gacaaggtgg aggaaaacac cacagcccac
     1681 gtacaagagc gctatggggc ttatgtatgg cttgctggta agaaatttaa ctaggagggg
     1741 gaatatgtcg acattttcgg ttgaatttga acaaggctta ttagatagag ttgataagct
     1801 agcacagcaa aagctagagc tagagaagaa gttacaaaag aaaacaggtt taatcacagc
     1861 caaggaactc aaagatgacc tcgatatttc tggcacaact ttgaaaaact ggatggatat
     1921 tgggctagtc tcatatcaat caccctttga aagtagcaaa aaactatatt tcaaggtttc
     1981 tgatgtgatt aattttctca cagtacgcta ggaggtatat tttgaaagta tttattattg
     2041 atggtgatgc gtggagaggt aaggcgcatt attcacctag cttagatgtt gtgtttatca
     2101 gtgacaaagt ggataagcac gcgcgtgatg aattgattga gcgtgtgacg aaatcaagta
     2161 atagaacaat ctggaggtaa aattgtggct aaagaacatt acgaagtaac aagtgtcttt
     2221 ccagcaggct ggattgttaa cggaaagaaa ctaacagaac ctattgaaat gtcagcagaa
     2281 gaactaagaa aatccgaggt atatccgcct gaaaacagtt cggtatatga cattttcaaa
     2341 aaaataaacg aagagagatg ggaggagacg cggaaagatg gcaacaaggc gaatgattag
     2401 caaggaagtt gttatgactg acaactttct tgatttacct cccactacaa aagttttgta
     2461 tttttttcta aacttagagg cagatgatga tggttttgtc ggaaatccta agactgttat
     2521 gagattaact gaaacaacta aggatgatat gaaactttta attgatagcg agtatgtgct
     2581 attgtttgat actggagtag tagttataac agattggaca gagcataacg ctatcagaaa
     2641 agacagaaaa aaagatacta gatttacaaa agaaatgcaa caaataatat tggtagacgg
     2701 tgggaaatat ttttggttgt cagatagtca accaaacgac aaccaatcgg caaccattaa
     2761 tacaccaaat gggtgcgtag gagaggatag gagaggtaag gatagtatag gtaaggagag
     2821 agtagtagaa gtagataaag aacaaaatca atcacctact ccccctcctt ttaatcaaga
     2881 ctttgtaaat ctctataaat cttttgaggc tgaaactggt aaagccttat cgccaataca
     2941 gatgcaagaa ttgcagtaca tgctagaaga ttttagcgct gatgttattc atgaggcatt
     3001 gagagaggca gtaggtcaag gtaaggctaa ttttgcttat attcaagcta ttctcaagcg
     3061 atggaaacag gataacctat tgacggtaga gcttattaga aataataagg cagcgcgtga
     3121 ggttaagaaa cagcaaaata attcaaaacc agaaccacaa acccgtgaag aatggttggc
     3181 gaactggtca gaagaaaacc cattttagaa aaaggagtta cacatgctaa cacaagctga
     3241 atttatcgca aacacaaaga cactagaaga gacttgcccc attcatggca tcccgttaat
     3301 gcaacttgat agagttgtta aaattgctga agaagataaa ccacggaagc tttctccgat
     3361 ttgtccagaa tgtataaaag agcaaaatga aaagcaagtg caggaagagg tagaaaaaca
     3421 cttgaacgca ggtatttatc agaaaaccta taacgtgctt atgcgtgaca gtaccatccc
     3481 agaagactta aaagcggcat cttttgataa tttcattatt gagacaccag aggaaaagca
     3541 gatgttagat tttgtaaaaa atcaaactca aaaatatctt gatggcatga atggaaatac
     3601 tttgctaaca ggtactacag gggttggaaa aactcatcta gctatttctc ttgccaaaga
     3661 gctaaacgag gcgtatagag ccaaaggaga gcctaaaagt gtattgttta ttaaccttac
     3721 tgagatacta agagaaatcc gagagagttt taagtttact agcaaagagg gttattattc
     3781 acggatgcta aaagaggttg attatctgat tttggatgat ttaggggtca aacttggcaa
     3841 cgcctcgggt caatccaagt cagcatggga ggaagagttt atttttgatg tacttagtca
     3901 tcggaataat actatcatca caactaacct aagtaatgat gagatagcaa acctttatag
     3961 cgaacgtgtt gcaagtcgtg tgcgtacagg cttagatggt aactatttca aagtatttag
     4021 tatcaaagat aagcgttact caatcaataa gctaaagagt aatatgtgat aatgaattaa
     4081 cttacaaacc taaacaaaaa tagacacttt tcatggggag agaaatcgcc ccaatttata
     4141 agtaatagcc atgctttttt ctacgaccaa ataaatcaag cgcggcaaac taatcaatt
//
LOCUS       DAZQCJ010000127         4152 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000127 DAZQCJ010000000
VERSION     DAZQCJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.129"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            20..940
                     /locus_tag="ACHU66_001960"
     CDS             20..940
                     /locus_tag="ACHU66_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFR3390214.1"
                     /translation="MLKKVIRGCFVALFGFVLAACSAQKEASQVQPGMKIVTSFYPIY
                     SLVKEVSGNKNDVRMIGSRQGIHSYEPSAADIKAIYDADVFIYHSRILESWAGRLEPN
                     LQGSSVKVLEASTNLPLTKVPGLEDMEAGQGIDEASLYDPHTWLDPVLVGQEAVSIGE
                     LLAESDPKNADYYRQNAATLEEKAQKLADKYSPIFLKATSKTFVTQHTAFSYTAQRFG
                     LKQLGIAGVSEEEPSPRQLAEIKEFVDTYNVQTIFTEKGASDKLAKALASSTGVDLKV
                     LDPLEADPENNLTYLENLEQVLETLAQELK"
     gene            974..4129
                     /locus_tag="ACHU66_001961"
     CDS             974..4129
                     /locus_tag="ACHU66_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HFR3390215.1"
                     /translation="MKKKAVVGSVAALVLGMSLCSYQLGRFQAMEEQKNRVSYIEDSQ
                     SVQTTVAEQLTPDQVSAKENIDAEQIVVKITDQGYVTSHGDHFHYYNGKVPFDAIFSE
                     ELVMKDPNYVLQDSHIINEVQDGYVIKVDGKYYLYLKDPSKHKNVRSKEEVERQKGIS
                     SADSKNQAAGNSKDGRYRTDDGYVFNPTDVIEDTGDGFIVPHGDHFHFIPKKDLSATE
                     LKAAQDYWNQKGSVSSASGSQYGDRNNRAQQTTISAGQGQDLASLLAQLDATPLSQRH
                     VEADGLVFDPRTITKKTAAGVIVPHGDHYHFIPYSQMSPLEEKISRMIGVNGAGVSSG
                     AQASHSQHTPTQPNRPVTPIGTVTTQPVSPTQPVLPTQPKQSTGKVVSYMGRQIPAYG
                     KGLDGKAYFTSDGYTFSKDSITSVDDQGLIASHGDHFHYVGFGELEDFEIKQVEEWVN
                     EKAGKQVPPKTSEQVGNDAKPTKPSQGNDSKPVKPIQEENRPAFEYKQVTAKRKLAGK
                     VVYEMEVGGKTYTYGRDELDLMKISFAELTLAEKDKQYIFDIAPLAEGDLKPAMLVGM
                     DQIPMKGANATYDTGQSFIIPHIDHIHVLPYTWLSKEQIATIRYIMQHPEIRPSAWTT
                     SGHGDGEATDLVPPILNATPKANRLGLKNWQIIHTAEEVMDARAKGKFATNDGYIFSA
                     EDVLDPASFVFSQAFSLPRATGGSLRSISKKDLSKEELEAVQTLLDKRDAEELAQNVT
                     PIEKRAGLKNWQIVHSAEELAEAKAAGKYTTKDGYIFDPADLLDPKVKIGTDNYRIPR
                     VITDGYRRINKSDLNYLSELIPAEAMVAQREKSNSSSPSTPAPTETGASAGETTKPEQ
                     PQVAKETAEEIYNRVEAKKVVPFEALTYNAGYATEVRNGTLVIPHQDHYHYVSFKWFD
                     QGSARSPEGYSLEDFLATVKYYMTNPQERPVSDDGWGVFTPNTPSESTEETETEESDE
                     EIISEETEEIDEFTEELKRRAEEFGMDFKTFEQSLVTLSDRYKVSFEAFEYDAASKVV
                     RLVDKDGVKRTISLPSLEEQV"
BASE COUNT         1385 a          795 c          941 g         1031 t
ORIGIN      
        1 gttaacaaag gaggtagtta tgttaaagaa agtgataaga ggctgttttg ttgctttatt
       61 cggttttgtt ttagcagctt gctcggctca gaaggaagcg agtcaagtgc agccaggaat
      121 gaaaatagtc acctcatttt acccgattta ttcactggtt aaggaagtgt cagggaataa
      181 aaatgatgtt cgaatgattg gctcaagaca gggtatacac tcttatgaac catcggctgc
      241 ggacataaag gccatatacg atgcagatgt ttttatctat cattcgcgaa ttttggaatc
      301 ctgggcagga cgtttggaac ctaatttgca aggttcatct gtcaaggttt tggaggcttc
      361 gacaaattta ccgctgacaa aggttccagg gttagaagac atggaagctg gtcaagggat
      421 tgatgaagct agtttatatg acccccacac atggctggat ccagttttgg ttggtcagga
      481 agctgtttcg attggtgaac ttttagcaga aagtgatcct aagaatgcgg attattatcg
      541 tcaaaatgcc gcaactttag aggaaaaggc gcaaaagttg gcagacaagt atagcccaat
      601 ctttttaaaa gccacatcaa aaacatttgt cactcaacat acagccttct cttacactgc
      661 acaacgcttc gggttgaagc agttgggaat cgcaggagtt tcagaggaag agccaagtcc
      721 tagacagttg gcggaaatta aagaatttgt tgacacctac aatgtgcaaa caatttttac
      781 agaaaaaggt gcctcggata agcttgccaa ggcattggcg agttcgacag gtgtagattt
      841 aaaagtgttg gatcctcttg aagcagatcc agaaaataat ttaacctatc tagaaaatct
      901 agagcaagtc ttagaaacat tagctcaaga gttaaaataa aataccgaat tctatcgacc
      961 gaaaggagaa aaaatgaaga aaaaagcagt tgttggctcc gtagccgcgc ttgttttagg
     1021 gatgagtctc tgtagctatc agcttggacg tttccaagcg atggaagagc aaaaaaatcg
     1081 tgtgtcctat attgaagata gtcaaagtgt acaaactacc gttgctgaac aattaacacc
     1141 tgatcaagtt tctgccaagg aaaatattga cgctgagcag atagtcgtta aaattactga
     1201 tcaaggctat gtgacatcac acggtgacca tttccactac tataatggta aagtcccttt
     1261 tgatgcgatt tttagtgaag agttggtcat gaaggaccca aactatgtct tacaagacag
     1321 tcacatcatc aatgaagtcc aggatggcta cgtcataaaa gttgatggga aatactatct
     1381 ctatctaaaa gaccctagca aacataaaaa tgttcgtagc aaagaagaag tggagcggca
     1441 aaaaggaatc tcttctgctg acagcaaaaa tcaggcagca ggaaatagta aagacggccg
     1501 ctacagaaca gacgatggct atgtctttaa tccgacagat gttattgaag acacgggaga
     1561 tggttttatc gttccgcacg gcgaccattt tcactttatt cctaaaaaag atttatcagc
     1621 taccgaattg aaggctgctc aagattactg gaatcaaaaa ggaagtgtga gctcggctag
     1681 tggtagtcaa tatggcgata gaaataatag agctcaacag acaacaatta gtgcggggca
     1741 aggtcaggat ttggctagtt tattggctca attggatgca acacccctat ctcaacgcca
     1801 tgtagaagca gatggattgg tatttgaccc aagaacgatt acgaagaaaa ccgcagcagg
     1861 tgtcatcgtt ccacacggcg atcattacca ctttattcca tacagccaga tgtctcctct
     1921 agaagagaag atttctcgaa tgattggtgt aaatggagca ggtgtttctt ctggtgcaca
     1981 agcaagtcac tcccaacata caccaacaca accaaatcgt ccagtaacac caatcggcac
     2041 ggttacaacg caaccggtat cgcctacaca accagtatta ccaacacaac cgaagcagtc
     2101 aacagggaaa gtggtttcct atatgggacg tcaaattcct gcttacggaa aagggttaga
     2161 tggtaaggca tattttacaa gtgatggata cacctttagc aaagactcta ttacctcagt
     2221 tgatgatcaa gggctgatag ctagtcatgg agatcatttc cattatgttg gttttggcga
     2281 actcgaagat tttgaaatca agcaagtaga ggaatgggtt aatgagaagg cgggcaaaca
     2341 agtgccgccg aaaacatctg aacaagtagg aaacgatgct aaacccacaa aaccaagtca
     2401 agggaatgat tcaaaacctg taaaaccaat tcaagaagaa aatcgccctg catttgaata
     2461 caaacaagta acggctaaac gtaaattggc tggaaaagtt gtttatgaaa tggaagtagg
     2521 tggaaaaact tatacatatg gtcgtgacga actagatttg atgaaaatct cttttgctga
     2581 gttgactttg gcagaaaaag ataagcagta tatctttgat attgcaccgc ttgcagaagg
     2641 agatcttaag ccagccatgt tggtcggtat ggatcaaatt ccgatgaagg gagcgaatgc
     2701 aacctatgat actggacaat cctttattat tccacacatt gatcacatcc acgtcctacc
     2761 ttacacatgg ttgagtaagg aacaaattgc aacgatccgc tatatcatgc aacatcctga
     2821 aattcgtcca tctgcttgga ctacaagtgg acatggtgat ggagaagcaa cggatcttgt
     2881 tcccccaatt ttaaatgcaa cacctaaagc caaccgctta ggcttgaaaa attggcaaat
     2941 cattcacaca gcagaagaag tcatggatgc acgtgcaaaa gggaaatttg cgacaaatga
     3001 tggctatatc ttctcggcgg aagacgtact agatccagca agttttgtct ttagtcaagc
     3061 gttcagtcta ccaagagcga caggtggttc tctgcgttca atttccaaga aagatttatc
     3121 taaagaagaa ttggaagctg tacaaactct tcttgataaa cgagacgctg aagaattggc
     3181 gcaaaatgtc acaccgattg agaaacgtgc aggtttgaaa aattggcaaa ttgttcactc
     3241 ggcagaagag ttggcagaag ctaaggcagc aggaaaatat acaacgaaag atggctatat
     3301 ttttgaccca gcagatctat tagatcccaa agtgaaaata ggtacggata actatcgcat
     3361 cccacgtgtg attacagatg gttatcgtcg gattaacaag agtgatttga attacttaag
     3421 tgagctaatc ccagcagagg ctatggtggc tcaacgtgag aaatctaatt ctagttcacc
     3481 gtcaacacct gcaccaactg aaacaggagc aagtgcaggc gagacaacaa agccagaaca
     3541 gccacaagtt gccaaagaaa cagcagagga aatttataac cgagtggaag cgaagaaagt
     3601 cgttcctttc gaagcactga cctacaatgc tggctatgcg accgaagtac gtaacgggac
     3661 tctggtaatt ccgcatcaag atcactatca ctatgtatcc tttaaatggt ttgaccaagg
     3721 tagtgcaaga agtcctgaag ggtatagttt agaagatttc ctagcaacgg ttaagtacta
     3781 catgactaat ccacaagaac gccctgtttc agacgatggc tggggagtat ttacaccaaa
     3841 tacaccatct gaatcaacag aagaaacaga gacggaagaa agtgatgaag aaattatttc
     3901 tgaggaaacc gaggaaattg acgagtttac agaagaatta aaacgaagag cagaagagtt
     3961 tggcatggac ttcaaaacct ttgaacaaag tctggtcaca ctctcagacc gttacaaagt
     4021 ttcttttgaa gctttcgaat atgatgcagc aagcaaagtt gttcgacttg tagataagga
     4081 tggggtcaaa cggaccatct cccttccaag tttagaagaa caagtatagt aaacaaaata
     4141 catgtagcgc tg
//
LOCUS       DAZQCJ010000128         4009 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000128 DAZQCJ010000000
VERSION     DAZQCJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.130"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(268..1227)
                     /gene="nrdF"
                     /locus_tag="ACHU66_001962"
     CDS             complement(268..1227)
                     /gene="nrdF"
                     /locus_tag="ACHU66_001962"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR3390216.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLTAEEKDLVGKVFGGLTLLDTLQSETGVQALRNDIRTPHEEAVYNNIQFMESVHAK
                     SYSSIFSTLNTKSEIEDIFEWTNSNEYLQRKAKIINEIYETGTPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELSEEEQDKLR
                     DWMYDLLYQLYENEEGYTRSLYDAVGWTEEVLTFLRYNANKALMNLGQDPLFPDSADD
                     VNPIIMNGISTGTSNHDFFSQVGNGYLLGEVEAMTDDDYLYGL"
     gene            complement(1357..3516)
                     /gene="nrdE"
                     /locus_tag="ACHU66_001963"
     CDS             complement(1357..3516)
                     /gene="nrdE"
                     /locus_tag="ACHU66_001963"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR3390217.1"
                     /translation="MSLKELGDVSYFRLNNEINRPVNGQIPLHKDQEAVKAFFKENVL
                     PNTKQFDSILDKIAYLLEENYLEKEFLDQYSPEFIIKIDQFLKDQNFRFKSFMAAYKF
                     YNQYALKTNDGAYYLESMEDRVLFNALYFAEGNEELALNLANEMIHLRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGISLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLDVFHPDIISFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARKNEDMYLFSPYSVELEYGVPYSYLDITEKYDELVANPRI
                     RKTKIKARDLETEISKLQQESGYPYVINIDTANRSNPVDGKIIMSNLCSEILQVQTPS
                     VINDSQEYLTMGTDVSCNLGSTNIVNLMKSPDFGRSVRTMTRALTYVTDHSHISAVPS
                     IEAGNSQAHSIGLGAMGLHSYLAQNLIDYGSKTAVEFTNIYFMLLNYWTLVESNNIAR
                     ERKATFHNFEKSKYADGTYFDKYVTGDYQPQSERVKELFEGIFIPSGQDWADLREKVK
                     ADGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLATETI
                     PFYKSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLRSELPKELYEWKKENKQTTRDLS
                     ILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI"
     gene            complement(3549..3767)
                     /gene="nrdH"
                     /locus_tag="ACHU66_001964"
     CDS             complement(3549..3767)
                     /gene="nrdH"
                     /locus_tag="ACHU66_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR3390218.1"
                     /translation="MVTIYSKNDCVQCKMTKKFLDQHSVDYKEINLDEQPEFIDHVKV
                     LGFSAAPVIETENDVFSGFQPGKLKALV"
BASE COUNT         1122 a          769 c          895 g         1223 t
ORIGIN      
        1 tattaacatt atatacgatt ttaaactcaa cagatttcta tagtctttta gtactaggca
       61 atgagccgca gacagtactg gagtgccgca cagttgatta ggttggattt agagtgtaaa
      121 acacgaacaa atccgccaat caaccactgc gctgagatgt tgacacgaac tctaagaagt
      181 ggcgctgggc tttttgccca gcctcacgtc aaagataaac aaaatgacta taaaacggaa
      241 aaatagaaat gatatttttc ctaagatcta caacccatat aaataatcat catctgtcat
      301 agcttccact tcaccaagaa gatagccgtt accgacttgg gagaagaagt cgtggttaga
      361 tgtaccggtt gagataccgt tcatgatgat tgggttgaca tcatctgctg aatctgggaa
      421 gagtggatcc tgacccaagt tcatgagggc cttgttggca ttgtaacgaa ggaaagtcaa
      481 gacttcctca gtccagccga ctgcgtcata gagggagcgt gtgtagcctt cttcattttc
      541 gtaaagttgg tagagcaggt cgtacatcca gtcgcggagt ttatcttgtt cttcctcgga
      601 caattcattg aaaccaagtt ggaacttgta accgatgtag gtaccgtgca cagactcgtc
      661 acggatgatc agcttgatga tttctgcaac gttggccagc ttgttgttac caaggtagta
      721 gagtggcgtg aagaaaccag agtagaagag gaaggtttct aggaatacgc tagctacttt
      781 cttttcaaga ggtgtacctg tttcgtagat ttcgttgata atctttgcct tgcgttgcaa
      841 atattcgttg ctgttggtcc attcaaagat gtcttcaatc tcagacttgg tgttcaaggt
      901 tgagaagata gaagagtaag acttggcatg gactgactcc atgaattgga tgttgttgta
      961 aacagcttct tcgtgcggtg tacgaatatc gttgcggagg gcttggacac ctgtttcaga
     1021 ttgaagggta tccaagaggg tcaatccacc aaagacctta ccaaccaggt ctttttcttc
     1081 agcagtaagt ttacgccagt catccaagtc gtttgataaa ggaatacgtg tatcgagcca
     1141 gaattgctcg gtcaactttt cccaagtaga cttgtcgata acatcctcaa tggcgttcca
     1201 gtttatggct ttgtagtagg tttccatcat attctctttc tttttcttat acttcaaata
     1261 ttatatcaca aatagatagt agcctgtaaa aaaatcccct aaaagggatt ttaatcgatt
     1321 gcttattagt tgcttcacat cgtgaacgat agtgtcttaa atcacacaac tttcacattg
     1381 gtttgcgccg acttcttcgc cgtcatcggt aaaggtacgg atgtagtaga tagacttgat
     1441 acctttgttg aaggcgtagt tacgaaggat agagaggtca cgtgtagttt gtttgttctc
     1501 ttttttccat tcatagagtt cttttgggag ctcgctgcga aggaaaagag tgagtgacaa
     1561 cccttggtcg acgtgttctg ttgcggcagc atagacatcg ataaccttcc gcatatccat
     1621 gtcataggca gatttgtaga atggaatggt ttctgttgcc aagccagctg ctggatagta
     1681 gattttaccg attttcttct cttggcgttc ttcaatccgt tgtgtgattg ggtgaattga
     1741 agcagaaacg tcgttgatat agctgataga tccatttgga gcaacagcta agcggttttg
     1801 atgatagaga ccgtctgctt ttactttttc gcggaggtca gcccaatctt gtccacttgg
     1861 aatgaaaatg ccttcaaaaa gttctttaac acgctcggat tgtggttggt agtcacctgt
     1921 cacgtattta tcgaaataag tgccgtctgc atattttgat ttttcaaagt tatggaaagt
     1981 agctttgcgt tcgcgagcaa tgttatttga ttctaccaag gtccaatagt taaggagcat
     2041 gaagtagatg ttggtaaatt ctacggcagt ttttgaaccg taatcaatca agttctgagc
     2101 taagtaagaa tgaagtccca tagctccaag accgatagaa tgtgcctgac tgtttcctgc
     2161 ttcaatagat ggtacagctg aaatgtgtga atgatctgtg acataagtca aagcacgagt
     2221 catggtacgg acagaacgtc caaagtcagg cgatttcatc aagttgacaa tgttggttga
     2281 accaaggtta catgatacat ctgtccccat tgtcaagtat tcctgagagt cattgatgac
     2341 gcttggtgtt tgaacttgaa ggatttccga acagaggttt gacatgataa tcttgccgtc
     2401 aacagggttg ctacggttag ctgtatcgat gttgataacg tatggatagc cagactcttg
     2461 ttggagtttg gaaatttctg tttccaagtc acgtgccttg atttttgtct tacgaatgcg
     2521 agggttcgcc accaattcat cgtatttttc tgtgatgtca aggtagctat aagggacacc
     2581 gtattccaac tcaactgagt atgggctgaa gaggtacata tcttcgtttt tacgtgccaa
     2641 ttcgtagaat ttatcaggaa cggtgatacc gagtgataag gtcttcacgc gaactttctc
     2701 atcggcattt tctttcttag tcgaaaggaa ggaaataata tctgggtgga aaacatcaag
     2761 ataaacgaca ccagcccctt ggcgttgccc aagctggtta gaataggaga agctgtcttc
     2821 gaagagtttc ataacaggaa caaccccaga agctgccccc tcatagccct tgattggagc
     2881 acctgcctca cgcaagttgc taagggaaat accaacacca ccaccgatac gggacaattg
     2941 cagagcagag ttgatagaac gtccaatcga gttcatatcg tcagtgacct ggatgaggaa
     3001 acaagacacc aactcaccac gacgggcacg gcctgcgttg aggaaagacg gtgttgcagg
     3061 ctggtaacga agatgaatca tttcgttggc caggtttagt gctaattctt cgttgccttc
     3121 agcaaaatac aaggcgttga agagaacacg gtcttccata ctttccaagt agtaagcccc
     3181 gtcgttggtt ttgagggcat actgattata gaacttgtag gctgccatga aagacttgaa
     3241 gcggaagttt tggtctttca agaattgatc aattttgatg ataaattctg ggctatactg
     3301 gtccaaaaat tctttttcaa gatagttttc ttctaataga taggcaatct tgtctaaaat
     3361 actatcaaat tgcttggtat ttgggaggac attttcttta aagaaggcct taaccgcttc
     3421 ttggtccttg tggagtggga tttgaccatt aacaggacgg ttgatttcgt tatttaaacg
     3481 gaagtaggac acatcgccta gctcttttaa actcatagtc gcaatctctt tctataaaca
     3541 gtagggagtt aaaccaaggc ttttagtttt cctggttgaa aacctgagaa aacatcattt
     3601 tcagtttcga taacaggtgc agctgagaaa ccgagaactt ttacgtgatc gataaattct
     3661 ggctgttcat ctagattgat ttctttataa tctacactat gttggtccaa gaatttcttg
     3721 gtcattttac actgtacaca atcatttttt gaatagatcg ttaccattca atttctcctt
     3781 tgtccatgag ggattaataa aaaatcccaa ttttgttacc aatataggat acaagatttt
     3841 aaggttaaag tcaagaagga agtttgttta aaatcacaaa atatagtgca caatattttt
     3901 ataagacacc atatattgta catctgtcca atgctgccaa gtcgaaaaat agcgtttaca
     3961 tagctgtctc tatttagttt gataatccta gcaatctctc ggtaaagta
//
LOCUS       DAZQCJ010000129         3966 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000129 DAZQCJ010000000
VERSION     DAZQCJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.131"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..346)
                     /locus_tag="ACHU66_001965"
     CDS             complement(<1..346)
                     /locus_tag="ACHU66_001965"
                     /inference="COORDINATES: protein motif:HMM:NF028198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5082 family protein"
                     /protein_id="HFR3390219.1"
                     /translation="MSKDAEIARCNQRILTTNSQISSTESTIATYKTKLEALKKAYEA
                     MKTTKSDFKDKKKSLSQLQRSADTSWSGNLFNTKYDKPLEDLISDVNDVIGKIDDNLD
                     RINNKINDYENKI"
     gene            complement(360..866)
                     /locus_tag="ACHU66_001966"
     CDS             complement(360..866)
                     /locus_tag="ACHU66_001966"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390220.1"
                     /translation="MNKTWLYLLIFLPITVPFCFCLYRWRREYVIRKYSTSTIIGKVV
                     AHREWISLRPPIIEYEVKGIIYRKALEYTSVVYGPKDISYPRDIEELRNYMLERKKLY
                     NIGLMSYNMYEIFPIGYEMTVMYNPKRPQLAFVERYAGGELFFRFVSLFYFLVIILIC
                     LFLFFVLI"
     gene            complement(869..1408)
                     /locus_tag="ACHU66_001967"
     CDS             complement(869..1408)
                     /locus_tag="ACHU66_001967"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390221.1"
                     /translation="MKNIILIVTSMFLLTACGNSSTNTETQSSVESSTSSSVEDKALT
                     LVHGKNTTQAGEVVQYVEDLLEKNPSLGAENAISLNYADATNSDGTTTYALFLVTNRT
                     ADRIEQNFDFTVNWSYDGTVIYENQKVLYEPDEYGVLEPNTTAIIFLPLPAESVDVVK
                     NMTDNSKISLSISDLAYVE"
     gene            complement(1422..2801)
                     /locus_tag="ACHU66_001968"
     CDS             complement(1422..2801)
                     /locus_tag="ACHU66_001968"
                     /inference="COORDINATES: protein motif:HMM:NF016617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T7SS effector LXG polymorphic toxin"
                     /protein_id="HFR3390222.1"
                     /translation="MTKKLDNTAISTLKTEFEASYSTAETSLYEIFKALSVLTQSVNF
                     RGAGAESHKLFLQEASLNYAYVLLEVTQTFKSYISEVEKGFLEYESEVSGKVNTDTVS
                     EYKTSLSGIAKQIELLKSDLTSPESIARSHGVSIVSTGAGNLATQFSTIDSNLEKVNQ
                     DLADTDSSLKGKATTVSEAISNLKSSLDSISSTYVTSTGKYNTSTIGQLTKQDWYLKG
                     NKDIFSDKQKSDPFVYNAGSASVLEGQYAVGLAKDTYLTTTGAVASGQYKAGIDGGEI
                     YLKSNGYVLGGSLEVQATSAIEVTAAASMASGNVDLRTGKYGWYASGGASGPSAQGKV
                     GIIDGLFSVSGQARFLFAEGTIGSYDNGEEFYYGFDASASLTEASANFDLFNVQIDGQ
                     KPVFAISAEPVLTGGGGATAAISGQSVYEDFLGLDWLDVDAVTFKVGGKAIIGGNISV
                     TIPWPDLNF"
     gene            complement(2803..3087)
                     /locus_tag="ACHU66_001969"
     CDS             complement(2803..3087)
                     /locus_tag="ACHU66_001969"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390223.1"
                     /translation="MSEQILINPEGFYPEIDNLSAAQALITDEMFSIDKGELNLDTVS
                     EIDSLVKELNSLYVLYREHLGRDVKNLNVVIAEWMKVDASLAGKNLLGGD"
     gene            complement(3080..>3966)
                     /locus_tag="ACHU66_001970"
     CDS             complement(3080..>3966)
                     /locus_tag="ACHU66_001970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390224.1"
                     /translation="YASQTIFSFISSSKKLIGDIDIFEKIKSKVPSREEFPELFKQFK
                     LDYTNNFSDEVKEEIIYSEFLHYHKRFSMDNFHKSIESLTFEDVEEVQKYLFQKIRYK
                     VYIYGNVEERIEDIQAWSVNGCSEFINPIILPLTDEEVVWKGIELNGISDRRSYLMHT
                     NLFQIPIADAYLTYQILSQWYSDKKIQITIDSIEMGAILPNTDNIEDFKLDLASIQLF
                     KDNILRQYEYLSGRKQLFYSEYFMKLWLAGLDYLDFYLYLQKISSEEIEDKMSTVLKV
                     SQFCKVELSKQNMEIIDE"
BASE COUNT         1215 a          823 c          577 g         1351 t
ORIGIN      
        1 tgattttatt ctcatagtca tttatcttat tgttgatacg atctaaatta tcatcaattt
       61 taccaataac atcgttaaca tctgagatca aatcttcgag tggtttatca tattttgtat
      121 tgaataaatt tccagaccaa ctcgtgtccg cgctcctctg taattgactc aatgatttct
      181 ttttgtcctt aaaatcagat ttggtcgtct tcatagcttc ataagctttt ttaagtgctt
      241 ctagttttgt tttgtaagtg gctattgttg attctgtact tgaaatctga ctattagtag
      301 ttaatatccg ttgattacaa cgagcaattt cagcatcttt tgacattaga aatacctcct
      361 tatattaaaa caaaaaacaa gaacaaacag ataaggatta ttacaagaaa atagaataag
      421 ctgacaaatc taaaaaataa ctctccccca gcataacgtt ctacaaaagc taactggggt
      481 cgcttcggat tgtacatcac tgtcatttca tatccaatag gaaatatttc atacatattg
      541 tacgacatta aaccaatgtt gtataacttt ttcctttcca acatataatt tcggagttct
      601 tctatatcac gaggatatga aatatctttt ggaccataaa caacagatgt gtattctagg
      661 gcttttctat atataatacc ctttacttca tactctataa ttggaggacg aagtgaaatc
      721 cattctctat gcgcaacaac tttaccgata attgtagaag tactgtattt tcgaataaca
      781 tattccctac gccatctata aagacaaaaa caaaaaggta cagttatcgg caaaaatatt
      841 aaaagatata accaagtctt gttcattttt actccacata agccaaatcc gaaatcgaaa
      901 gtgaaatttt gctattatct gtcatatttt ttacaacatc aacactctca gcagggagag
      961 gtaggaagat aatggctgtc gtattgggct ccaacactcc atattcatca ggctcataca
     1021 gcactttttg attttcatag atgaccgttc catcatagga ccagttaacc gtaaaatcaa
     1081 aattttgttc aatcctatct gccgtcctgt tcgttactaa gaacagggcg taggtagtgg
     1141 ttccatctga attcgtagca tccgcataat ttaaactaat ggcattttct gctcctaagc
     1201 taggattctt ttcaagcaaa tcctcaacat attgaacaac ttcaccagct tgagttgtat
     1261 tttttccatg tactaatgtc aacgccttat cctccactga actacttgta ctactttcaa
     1321 cacttgattg tgtttctgta tttgtactcg aatttccaca agctgttaat aagaacatgc
     1381 ttgtgacaat taagataata tttttcatga tttcttactt tctaaaaatt gaggtctggc
     1441 caaggaattg taaccgagat attcccacca attattgcct ttccacctac tttgaatgtc
     1501 actgcatcaa catctaacca atctagccct aaaaaatctt catatacaga ttgacctgaa
     1561 attgcagccg tcgctccgcc tccaccagtt aaaacaggtt cagctgaaat tgcaaaaact
     1621 ggcttctgtc catctatttg tacattaaat aaatcgaaat ttgcacttgc ttcagtaagg
     1681 ctggcactag catcaaatcc gtaatagaat tcttcgccgt tatcatacga accaattgtc
     1741 ccctctgcga aaagaaacct agcttgacct gagacagaaa atagcccatc tataattcca
     1801 acttttcctt gtgcagatgg tccacttgca cctccgctag cgtaccatcc atattttccg
     1861 gtgcgcaagt ctacattacc tgatgccata ctagcagctg ctgttacctc aatagcagaa
     1921 gttgcttgaa cttctagact acctcccaaa acatatccat tagattttaa atatatttct
     1981 ccaccatcaa tccctgcttt gtattgtcca gatgctacag ctcctgttgt agtaaggtat
     2041 gtatccttcg ccaaaccaac cgcatactgc ccttccaaca ctgaggccga ccctgcattg
     2101 taaacaaagg ggtccgactt ctgtttgtcc gaaaatatat ccttgtttcc ttttagatac
     2161 caatcttgtt tcgttaactg accaatagtt gaggtgttgt atttacctgt tgaagtgacg
     2221 taggtagaag aaatgctatc taaacttgat ttcaaattag aaattgcttc ggaaactgta
     2281 gttgctttcc ctttcaagga actatccgta tctgctaaat cttggttcac tttctctaaa
     2341 ttagaatcaa tcgtagaaaa ttgagttgct aaatttccag caccagttga aacaatgctc
     2401 actccatgac ttctcgcgat tgattctgga gaagtcaaat cactttttag taattctatc
     2461 tgcttggcta tacctgacaa agaagtctta tactcagaaa ctgtatcagt attcactttt
     2521 cctgaaactt ccgactcata ctccaagaat cccttctcaa cttctgagat ataggattta
     2581 aatgtctgcg tcacctctaa taaaacatag gcgtagttta aactggcttc ttgtaaaaac
     2641 aatttatgag attctgcacc tgctccacga aagttcactg actgcgttaa aacggacagt
     2701 gctttgaaaa tctcataaag actggtttcc gcagtggagt atgaagcttc aaattctgtc
     2761 ttaagggttg atatagcagt attatctaac ttctttgtca tcttaatccc ccccaagcaa
     2821 attttttcct gcaagcgagg catctacctt catccactct gcaattacta catttagatt
     2881 ttttacatcc cgacctagat gctctctata aagtacataa agtgaattca attcctttac
     2941 taaactgtca atctctgaaa ctgtatccag attaagctca cccttatcta ttgaaaacat
     3001 ttcatccgtt ataagagctt gagccgctga caaattatct atttctggat agaatccctc
     3061 tggatttatt aatatttgct cactcatcta ttatctccat attctgtttt gatagttcaa
     3121 ctttgcaaaa ttgacttacc tttagtacag tagacatttt atcttcaatc tcctccgaac
     3181 ttattttttg taagtacaga tagaagtcaa ggtaatctaa accagccagc catagtttca
     3241 taaagtattc agaataaaat aattgctttc ttccgcttaa atactcatat tgccttaaaa
     3301 tattgtcctt aaatagttgg atactggcta ggtctagttt aaaatcttca atattatcag
     3361 tatttggcag tatagctccc atttctatag aatcaatggt aatttgtatc tttttatcac
     3421 tataccattg acttagaatt tgataggtca agtaagcatc tgcgattgga atttggaata
     3481 aatttgtatg catcagataa cttcgcctat ctgatattcc attgagttca atacctttcc
     3541 agacaacctc ttcatcagta agcggtagaa tgattggatt aataaactct gaacaaccgt
     3601 ttaccgacca agcctgtata tcttcaatcc tttcttcaac atttccataa atatatactt
     3661 tatatcgaat tttttgaaaa aggtattttt ggacttcctc tacatcttcg aatgtaagac
     3721 tctcaatgga tttatgaaaa ttatccattg agaaccgttt atgataatgt agaaattctg
     3781 agtaaattat ttcttctttc acttcatcag aaaagttatt tgtgtaatct aatttaaatt
     3841 gtttaaataa ctccggaaat tcttctcgtg acggaacttt tgactttatt ttttcaaaaa
     3901 tatctatgtc accaatcaac ttctttgaac tactaatgaa tgaaaaaatc gtttgagaag
     3961 cgtaca
//
LOCUS       DAZQCJ010000130         3958 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000130 DAZQCJ010000000
VERSION     DAZQCJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.132"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            56..442
                     /locus_tag="ACHU66_001971"
     CDS             56..442
                     /locus_tag="ACHU66_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFR3390225.1"
                     /translation="MDIHLRNEIDLDGQIEVVDQQFQVEVKEKDGNLYLIYSNDEAEK
                     VVIKCDEEELVMTRFSTPKSIMRFISGKEAIVTIPTPLGIQHFVTDTKRYQLNRSDQS
                     VQLQYELKGLENQQLFASYDLEISWK"
     gene            652..3339
                     /locus_tag="ACHU66_001972"
     CDS             652..3339
                     /locus_tag="ACHU66_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFR3390226.1"
                     /translation="MSKEQKRDLFYTQGEEQVLSSMESSLDGLSSDEASKRLADYGRN
                     ELDEGEKRTLLAKFLDQFKDLMIIILIAAAILTVITEGSHGLTDAIIILTVVILNAAF
                     GVYQEGQAEAAIEALKNMSSPVARALRDNHVVEVDSKDLVPGDIVLLEAGDVVPADMR
                     LLEANSLKIEEAALTGESVPVDKDLSAVLAEDAPIGDRVNMAYQNSNVTYGRGLGVVT
                     NTGMYTEVGHIAGMLANADETDTPLKQNLHQLSKVLTYAVLVIAAVTMAVSVFVRGEG
                     ILPALMTSVALAVAAIPEGLPAIVTVVLSLGTQVLAKRNSIIRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGQLVDAKEALDASNTTLRVMNFANDTKVDPTGKLIGDP
                     TETALVQFGLDQNFDVREVLVSEPRVAELPFDSTRKLMSTVHQQAAGNFFVAVKGAPD
                     QLLKRVTQIEENGTIRPITDADKQAILATNKDLAKQALRVLMMAYKYVDAIPELESDV
                     VENDLVFSGLVGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGIIDP
                     NDTEDHVFTGAELNELTDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNDGKVVAMTGD
                     GVNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKT
                     IQYLLSANTAEVLCIFLATLFGWDVLEPVHLLWINLVTDTLPAIALGVEPAEPGVMQH
                     KPRGRNSSFFSGGVLSSIIYQGILQTALVLGVYGFALLYPEHAGDNHAIHADALTMAY
                     VTLGLIQLVHAFNVKSVYQSIFTVGPFKNKLFNWSIVAAFLLLMATVVIPGFNTFFKV
                     SILTPTQWMVTVIGSGLMVVVVEIVKFVQRKLGLDEKAI"
     gene            complement(3455..3844)
                     /locus_tag="ACHU66_001973"
     CDS             complement(3455..3844)
                     /locus_tag="ACHU66_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390227.1"
                     /translation="MRVIIHYPKPIKQLSSQKLPLLVDGKLAGTVTQGKILSLPITQP
                     SVTLSIRGDKKSAIQVKEGDRVQIMENSRYFILYYFSLAIVPIALLFNLPTLVKTILL
                     ILALAITLLGQAIFPKYIISTEKSSDH"
BASE COUNT         1107 a          751 c          892 g         1208 t
ORIGIN      
        1 ttactctgct tcgttcatcc tttgttacta ggattaaaaa atagtaggag gaactatgga
       61 tattcattta cgaaatgaaa ttgatctgga cgggcagatt gaggtagtgg atcagcaatt
      121 tcaagtggaa gttaaggaaa aagatgggaa tctctatctc atttatagta acgatgaagc
      181 tgaaaaagtt gtcataaaat gtgacgaaga agagttagtg atgacacgtt tttccactcc
      241 gaagtctatt atgcgattca tttctggtaa agaagctatt gtgaccattc ctacaccgtt
      301 aggaatccag cattttgtga ccgatacaaa acgttaccaa ttaaaccgtt cggatcaaag
      361 tgtccagttg caatatgaat taaagggatt ggagaaccag cagctttttg cttcctatga
      421 tttggaaatc tcgtggaaat aatctcttat ttccagaaaa atattcttga atttctcggg
      481 atttttgata taatagttaa caaatcgagg gagtagctag cggaaaattc cgtgatattg
      541 tcgtcaatcc gacttgtttc cggcaatata ttaaatagcg agacttgttt taataaaaca
      601 ggtctctttt tattgtagaa ggtagagaag aggccgaaaa ggagaaggaa attgtcaaaa
      661 gaacaaaaac gcgatttgtt ttatacacaa ggcgaagagc aagtattgtc aagcatggag
      721 tcttctttag acggtttgtc tagtgatgag gcaagcaaac gtctagcaga ctacggtcgt
      781 aacgaattgg atgagggtga aaaacgcaca ctcttagcta aattcttgga tcagttcaaa
      841 gacttgatga ttatcatttt gattgcagca gcaatcttga cggtgattac agaaggttca
      901 cacggtttga cagatgccat tatcatcttg accgtggtta tcttgaacgc tgcctttggt
      961 gtttatcaag agggtcaagc tgaggcagct attgaggcgc taaaaaacat gtcaagccca
     1021 gttgcgcgtg cgcttcgtga caatcatgta gtcgaagttg attctaaaga cttggtacca
     1081 ggtgacatcg tcttgcttga agcaggtgat gttgtccctg ccgatatgcg tttgttggaa
     1141 gctaactccc tcaaaatcga agaagcagct cttacaggtg agtctgtacc agttgataaa
     1201 gatttgtcag cagttttggc tgaagatgca ccaatcggtg accgtgtcaa catggcttac
     1261 caaaactcta atgttactta cggccgtggt cttggtgtgg ttacaaatac aggtatgtac
     1321 acagaggttg ggcacatcgc tggcatgctt gctaatgcag atgagactga tacaccattg
     1381 aagcaaaact tgcaccagct atcaaaagtc ttgacatacg cagttcttgt aattgcagcg
     1441 gtaacgatgg ctgtttcagt attcgtacgt ggagaaggta tcttgcctgc tcttatgact
     1501 tctgtagctc ttgcggttgc tgccattcca gaaggtttgc cagctattgt tacagttgtt
     1561 ctttcacttg gtacacaggt cttggctaag cgtaattcca tcatccgtaa gttgcctgct
     1621 gttgaaacac ttggttcaac tgaaatcatc gcttcagata aaactggtac tttgactatg
     1681 aaccagatga ctgttgaaaa agtttataca aacggtcaat tggtggatgc taaagaagct
     1741 ttggatgcaa gcaatacaac gcttcgcgtg atgaactttg ccaacgacac aaaagttgac
     1801 ccaactggta aattgatcgg tgacccaact gaaacggcac ttgttcaatt tggtttggat
     1861 caaaactttg acgttcgtga agtcttggta tctgagcctc gtgtggctga attgccattt
     1921 gactcaacac gtaaattgat gtcaactgtt caccaacaag ctgctggaaa cttctttgta
     1981 gctgttaaag gtgccccaga ccaattgctc aaacgtgtaa cgcaaattga agaaaacggt
     2041 acaatccgtc cgattaccga tgcggataaa caagccattc ttgccactaa caaggacttg
     2101 gctaaacaag cccttcgtgt cttgatgatg gcatacaagt atgttgatgc tattcctgaa
     2161 ttggaatctg acgttgttga aaacgacctt gtcttctcag gtttggttgg tatgattgac
     2221 cctgagcgtc ctgaagcggc ggaagctgtt cgtgttgcta aggaagctgg tatccgtcca
     2281 atcatgatta cgggtgacca ccaagataca gctgaggcga ttgcaaaacg tcttggtatc
     2341 atcgatccaa atgatacaga agaccatgta tttacaggtg cagagctcaa cgaattgacg
     2401 gatgaagagt tccaaaaagt cttcaaacaa tattctgtat acgctcgtgt atctccagaa
     2461 cataaggttc gtatcgttaa ggcttggcaa aatgatggta aggttgttgc catgactggt
     2521 gacggtgtta acgatgcgcc atcacttaag actgccgaca ttggtatcgg tatgggtatc
     2581 actggtacgg aagtttcgaa aggtgcctct gatatggtcc ttgcagatga caactttgca
     2641 actatcattg tggcggttga agaaggacgt aaagtcttct caaacatcca aaaaaccatc
     2701 cagtatctct tgtcagctaa cactgctgag gtattgtgta tcttccttgc taccctcttt
     2761 ggttgggacg tattggagcc agttcatctt ctttggatca atttggtaac agatacactt
     2821 ccagcgattg cccttggtgt tgaacctgct gaaccaggtg ttatgcagca caaacctcgt
     2881 ggtcgtaact caagcttctt ctcaggtggt gtcttgagtt cgattatcta tcaaggtatc
     2941 cttcaaacag cacttgtctt gggcgtatat ggttttgcac ttctttaccc agagcatgct
     3001 ggagataacc atgccattca tgcggatgct cttacgatgg catatgttac tcttggattg
     3061 attcagttgg tgcatgcctt caacgttaag tctgtttacc aatctatctt taccgttggt
     3121 ccattcaaga acaaactctt taactggtct atcgtagctg cattcttgct cttgatggcg
     3181 actgttgtga ttccaggatt caataccttc ttcaaggtat ctatcttgac accaactcaa
     3241 tggatggtaa cagtaatcgg ttcaggtctc atggttgtcg ttgtagaaat tgttaaattt
     3301 gttcaacgta agttgggatt ggatgaaaaa gctatctaat atagttattc agcttatctt
     3361 ttattacttc gttaactcga cttgccgtac ttcagtacag cctgcgcctc gttaccttgt
     3421 actaaaagca aatatcattt gaaaaagctc agatttagtg gtctgagctt ttttctgtac
     3481 tgataatgta ttttgggaag attgcttgtc ccaacaacgt aatggctaat gctagtatga
     3541 ggaggatggt tttgacaaga gttggtaagt taaacagtaa ggcgattggt acgatagcca
     3601 agctgaagta gtagagaatg aagtacctgc tattttccat tatttggacc ctatcgcctt
     3661 ccttaacttg aatggctgat tttttatccc ctcgaatgct tagtgtaaca gatggttgtg
     3721 taattggtaa gcttagaatt ttaccttgag taactgtccc agcaagtttt ccatctacca
     3781 ggagcggaag tttttgggag gatagttgtt tgataggttt tggataatga ataataactc
     3841 tcatatgaac ctccttgtaa tctagtttca ttatagcaaa aaaagaaaaa atgtaaacgg
     3901 tttctgacac tgttttcccc tacaggcttg cttgaagaat aagaaactgc tataattt
//
LOCUS       DAZQCJ010000131         3951 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000131 DAZQCJ010000000
VERSION     DAZQCJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.133"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            65..994
                     /locus_tag="ACHU66_001974"
     CDS             65..994
                     /locus_tag="ACHU66_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR3390228.1"
                     /translation="MEKIIITATAESIEQVKELLVAGVDRIYVGEADHALRIPTNFTY
                     DELREIAELVHSAGKELTVAANALMHQDMMDNIKPFMELMREIKVDYLVVGDTGIFYI
                     NKRDGYNFKLIYDTSVFVTSSRQINFWKDHGAVEAVLAREIPSEELFDIAKNLEMPAE
                     ILVYGASVIHHSKRTLLQNYYNFTKADETDLSRGRGLFLAEPSDPDSHYSIFEDKHGT
                     HIFINNDIDMMTKLSELDEHGYHNWKLDGIYCPGQNFVEIVKLFVQARDLIEKGEFTY
                     DQAFLLDEQVRKLHPAERGLDTGFYEFDRNKVK"
     gene            1172..2458
                     /locus_tag="ACHU66_001975"
     CDS             1172..2458
                     /locus_tag="ACHU66_001975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR3390229.1"
                     /translation="MSKTLKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFTMDEMREGIEYAHARGAKVYVAANMVTHEGNEEGAGAWFRELRDMGLDAVIVSDP
                     ALIVICATEAPGLEIHLSTQASSTNYETFEFWKELGLTRVVLAREVTMDEVAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSKRDANRGGCSQSCRWKYNLYDLPFGEERRSI
                     KGEIPEEFSMSAVDMCMIDHIPDMIENGVDSLKIEGRMKSVHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLIDEMWKVAQRELATGFYYSPPSENEQLFGARRKIPEYKFIAEVV
                     AFDKETMTATIRQRNVINEGDQVEFYGPGFRHFETTIQDLHDSNGEKIDRANKPMELL
                     TIKLDREIYPGDMIRACKSGLINLYQEDGQSLTIRA"
     gene            complement(2671..2994)
                     /locus_tag="ACHU66_001976"
     CDS             complement(2671..2994)
                     /locus_tag="ACHU66_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390230.1"
                     /translation="MKRFLEVIFEQITVFLEHGKAPKSIEPDSKEEIKNNLELALYKK
                     AAVHVIYGDRSFTGDIVKYDTDCQRIIMKNFKRQVTSIIKIEDIKKVTLVPDSIKTSQ
                     QQTKG"
     gene            3086..3301
                     /locus_tag="ACHU66_001977"
     CDS             3086..3301
                     /locus_tag="ACHU66_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018167072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFR3390231.1"
                     /translation="MAEFTFEIEEKLLVLSENDKGWTKELNRVSFNGAPAKYDIRTWS
                     PDHTKMGKGITLSNEEFQVLLDAFANK"
     gene            complement(3313..3852)
                     /locus_tag="ACHU66_001978"
     CDS             complement(3313..3852)
                     /locus_tag="ACHU66_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFR3390232.1"
                     /translation="MKTTSIKSLVYIAIGTALISTLSQISLSIGPVPFTLQTLAIGLV
                     ACLYKPKEAITSVSLYLVLGAIGLPVFSGFSGGFAALTGPTSGFLWGFLLYTIITSAL
                     TKSDSSPVTIFLACLLGTSICFLLGCFVFKLVLGASWTDSIGWTVLPFILPDLAKITL
                     VMICHRLLQPVIKKEAYFA"
BASE COUNT         1212 a          756 c          877 g         1106 t
ORIGIN      
        1 gtctgaacat taaaatacag gtagtcactt atcagatatt tctctttttg atataattat
       61 ctttatggaa aaaattatca ttacagcaac agctgaaagt attgagcagg tcaaagaact
      121 gcttgtagca ggcgttgacc gcatttatgt aggtgaagct gaccatgctc ttcgcatccc
      181 aacaaatttt acctatgatg aactcaggga aatagcagag ctagttcata gcgctgggaa
      241 ggaattgacg gtcgctgcca atgccctcat gcatcaagat atgatggaca atatcaaacc
      301 atttatggag ctaatgaggg aaatcaaggt tgattatctg gttgtcggag atactggaat
      361 tttctatatc aataaacgtg atggctacaa tttcaagcta atctatgata catctgtctt
      421 tgtgacttcc agtcgtcaga ttaacttttg gaaagatcat ggagcagtag aggctgtttt
      481 ggcaagagag attccatcag aagaactctt tgatattgct aagaacttgg aaatgccagc
      541 ggaaatcttg gtctacggcg cctctgttat tcaccattct aaacgaacct tgttacagaa
      601 ttattacaat tttacaaagg ctgatgagac agatttatca cgtggacgcg gacttttctt
      661 agctgagccg agcgacccag atagccacta ttctatcttt gaggacaagc acggtaccca
      721 tatctttatc aacaatgaca ttgatatgat gaccaagctg tcagaattag atgaacatgg
      781 ttatcataat tggaaacttg atggtatcta ttgccctgga cagaactttg ttgaaattgt
      841 taagcttttt gttcaagcaa gagatttgat tgaaaaaggc gaattcactt atgatcaggc
      901 cttccttttg gatgagcagg tacgcaagct ccatccagct gaacgtggct tagatacagg
      961 cttctacgaa tttgaccgca ataaggttaa ataacaatat ttatagcaca atttatttac
     1021 tttaacctga cagagttagc tctgtcactt attatgtaag gtcagagcac gaaaacgcct
     1081 ttgagcgatt ttctaaactc tgcactaggt ttccacatgg aaaagtatcg tttcatgtgg
     1141 aaaccgtcgc aaatagagga gattttacat gatgtcaaaa acattaaagc gtcccgaggt
     1201 cttgtcacct gctgggactt tggagaaatt gaaggttgcc attgattatg gtgctgatgc
     1261 ggtctttgtt ggcggccagg cctacggtct gcgtagccga gctggtaact ttaccatgga
     1321 cgagatgcgg gaagggattg aatatgccca tgcccgtggt gccaaggtct atgtggctgc
     1381 caacatggtc acccacgaag gtaatgaaga aggagcagga gcctggttcc gtgaattgcg
     1441 tgacatgggc ttggatgccg ttatcgtatc tgacccggct cttattgtta tctgtgctac
     1501 tgaagcgcca ggccttgaaa ttcacctgtc aacccaggct tcttccacca actatgagac
     1561 cttcgagttc tggaaggaat tgggcttgac tcgtgtcgtc ctggcccgcg aagtgactat
     1621 ggacgaggtg gctgaaatcc gtaaacggac agacgttgag attgaggcct ttgttcacgg
     1681 agctatgtgt atttcctatt caggtcgctg cgttctctct aaccacatga gcaaacgcga
     1741 tgctaaccgc ggtggctgct cccagtcttg ccgttggaag tacaatctct acgacctccc
     1801 atttggcgaa gaacgccgtt cgattaaggg ggaaatccca gaggagttct ccatgtctgc
     1861 agtggacatg tgtatgatcg accatattcc agatatgatt gaaaatgggg tcgatagcct
     1921 gaaaatcgaa ggccgtatga aatctgtcca ctacgtatca acagtgacca actgctacaa
     1981 ggctgcggta gatgcctatc ttgaaagccc agaaaagttt gaagcaatca agcaagacct
     2041 gattgatgag atgtggaagg ttgcccaacg tgaattggcg acaggtttct actatagccc
     2101 gccgagtgaa aatgagcagc tctttggagc ccgccgtaag attccagaat acaaattcat
     2161 cgccgaagta gtggcttttg acaaggaaac catgactgca accattcgtc agcgtaatgt
     2221 tatcaacgaa ggtgaccaag tagagtttta cggaccaggt ttccgtcatt ttgaaacgac
     2281 tattcaggac ttgcatgatt caaatggaga aaagattgac cgcgccaaca agccgatgga
     2341 actcttaact attaagctgg accgtgaaat ctatcctggt gacatgattc gtgcctgcaa
     2401 atcaggcctc atcaacctct accaagaaga tgggcaatct ctaactattc gtgcataaac
     2461 ataaaaaggc tgaaatttca gcctcagaac gtagataaac tcttaaaaat ataaaaaatt
     2521 gagaagtttg cgacagatag caagtcatta aactcttaga aacattgata tttcggcgtt
     2581 tctgagagtt tttaattttc aacataaaga aaaaagattg aagaaaattg tccaaaatgg
     2641 actttttctt caatctgcaa ggctgaaatt tcagcctttt gtttgttgtt gagaagtctt
     2701 gatgctatct ggaaccaagg tgactttttt gatgtcctcg atttttataa tgctggtaac
     2761 ctgacgtttg aaatttttca tgataatccg ttggcaatcg gtatcatatt ttacaatgtc
     2821 acctgtgaaa ctacgatcgc catagatgac atggactgca gcttttttat ataaagccag
     2881 ctccaagtta ttcttgattt cttccttgct atctggctca atagacttag gagctttgcc
     2941 atgttctaaa aagacggtga tctgctcgaa aataacttct aaaaacctct tcatggcata
     3001 atcctttcaa acttgatata attatattat ctaattttta taaaaatgac aagctttttc
     3061 gaatagataa gtgaggaggt aacaaatggc agaatttaca tttgaaattg aagaaaaatt
     3121 attggtctta tctgaaaatg ataaaggatg gaccaaggag cttaatcgag tatcttttaa
     3181 tggcgcacca gccaagtatg acatccgtac ctggagtcct gaccatacca agatggggaa
     3241 aggaatcaca cttagtaatg aggaatttca agttctactc gatgcgtttg ccaataaata
     3301 aacaaaggaa gcctaagcaa aataggcttc ttttttgata acaggttgca ataaacgatg
     3361 acaaatcatg actagtgtaa ttttagctaa atcaggaaga atgaaaggca gaacagtcca
     3421 tccgatagag tcagtccaac tggcacctaa aactagctta aatacaaaac agccgagaag
     3481 gaaacagata cttgttccaa gtaggcaggc tagaaaaatt gtgactggtg aggagtctga
     3541 tttagtgagg gcagaagtga tgatagtgta gagtagaaac ccccatagaa agcccgaggt
     3601 cggtcctgtg agtgcagcaa agccaccaga aaaaccagaa aaaacaggta aaccgatagc
     3661 accaagaact agatataagc tgacacttgt aattgcctcc ttgggtttgt atagacaagc
     3721 tactaagccg atagctaggg tttgtaatgt aaatggtaca ggtccgatag acagactgat
     3781 ttgtgaaagg gtggaaatta aggctgttcc aatagcgata taaacaagtg atttgataga
     3841 agttgttttc atttgttatc ctcttaaaat tttatggtta acaaaaatta tagtgagatt
     3901 tgataagaaa gtcaatgctc tctcatcggc tgtaacaact ctataggtga t
//
LOCUS       DAZQCJ010000132         3833 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000132 DAZQCJ010000000
VERSION     DAZQCJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.134"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(19..252)
                     /locus_tag="ACHU66_001979"
     CDS             complement(19..252)
                     /locus_tag="ACHU66_001979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390233.1"
                     /translation="MYLRFVLIDISDDGFYHYEIYPENKEEHKQTLVFNPETKDIRVN
                     TFDDASMKYLGKFLQNFKNQDGTYRKELSFGWG"
     gene            complement(257..1477)
                     /locus_tag="ACHU66_001980"
     CDS             complement(257..1477)
                     /locus_tag="ACHU66_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014073236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390234.1"
                     /translation="MNDDILPSLLKEVQDKFEASYGKSDIISSAFAKLKNKKATYSTA
                     NDFAIELGDILAEALGSSVAGDRLPDGKMYYNIGQRLLNDVLGKNFELVSDFTGQVQA
                     NLNKEANIGLKVQVPKFNQDRVDGLVNRLSSEVDFEKVSWLLKEPIVNFTQSIVDDSI
                     KTNADFHAKAGLTPKVVRKESGNCCKWCRAVVGVYNYPDVPKDVWRRHNRCRCTVDYH
                     PGNGKKQNAHSKRWSDPLKNAKIEERKGIGLLVQAGAKNYVRDDSNDELLPKEFIKAE
                     KHAYLTYDRIKNSNQDLEKRKIYSNIWKFKEMNGFSKDDVDKAFDHVFNNVHELNYGK
                     GLFAPDIDMAQSWDRLISGKNIQPHDLILLKHERLEHDYMYVSSKLDYDTAHKKVDEL
                     FNYSEAVDEFKKKG"
     gene            complement(1470..2735)
                     /locus_tag="ACHU66_001981"
     CDS             complement(1470..2735)
                     /locus_tag="ACHU66_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR3390235.1"
                     /translation="MSYKGMSYLRKKLALYQLGVKKRYRFYAMADRDNTKSFIIPDNV
                     KSMYNSVMGWTAHSVDALADRIIFREFANDDFEATEIFDANNPDIFFDTAIQSALIAS
                     CCFVYLVPNPDGLPKMQVIEASKATGVIDTTTFLLTEGYAILEVDENDNPVLEAYFTK
                     DVTWYYPKGANPYSISNPTGQPLLVPIIHRPDAVRPFGRSRITRAGMYNQLAAKRTLE
                     RAEVTAEFYSFPQKYVLGTSQDADPMEKWKTTVSSLLEITKDDDGDSPNVGQFNTASM
                     APFIDQLRMYASLFAGGSGLTMDDLGFPSDNPSSVEAIKAAHENLRAAGRKAQRSIAS
                     GLLNVAYVAVCLRDRYQYTREQFLNTKIKWEPLFEADANMLTMIGDGAIKLNQALPGY
                     INAETIRDLTGIAGDMSATPKLAEVVANE"
     gene            complement(2732..3091)
                     /locus_tag="ACHU66_001982"
     CDS             complement(2732..3091)
                     /locus_tag="ACHU66_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390236.1"
                     /translation="MKKSCAFCGRSFTAESKRVKYCSDKCRKDGARDKQRKLMKQKRA
                     NLKKQKSKKDNPNNQPAKKRKKKKNLLKYYQDFKTKILANEAEFGFTSRTIVEGVEVH
                     EPDFEEQVINKIKEQPK"
     gene            complement(3183..3530)
                     /locus_tag="ACHU66_001983"
     CDS             complement(3183..3530)
                     /locus_tag="ACHU66_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR3390237.1"
                     /translation="MRADKSGTHRVAFEKNKKRILKTATHCGICGQLVDKKLKYPNPM
                     CAVIDHVVPLAKGGHPSLIENLQLAHMSCNRIKSDKLFADNSKAEQKTLGNRNLPQSR
                     DWSQFISVKSEGG"
     gene            complement(3627..>3833)
                     /locus_tag="ACHU66_001984"
     CDS             complement(3627..>3833)
                     /locus_tag="ACHU66_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFR3390238.1"
                     /translation="RELQSMIDGLSNSDSQTVLSMVYIDKMSPWQVMDALNCSESTYY
                     RILRVATRELNEMTVNDRDLQKIQ"
BASE COUNT         1035 a          865 c          674 g         1259 t
ORIGIN      
        1 actcgatatc tcgaatgctt atccccaccc aaacgacaac tctttacgat aggtcccgtc
       61 ttggttttta aaattttgta aaaatttccc taaatacttc atgctcgcat cgtcgaatgt
      121 atttacacga atatcttttg tttcaggatt aaaaacaagc gtttgcttat gctcctcctt
      181 attttcaggg taaatctcat agtgataaaa accatcgtca ctaatatcta tcaaaacaaa
      241 tcttaaatac atacctttac cctttctttt taaattcgtc gacagcttcg gagtaattaa
      301 ataattcatc aacttttttg tgagctgtat cataatccaa cttgctactc acatacatat
      361 aatcatgttc taagcgttca tgcttcagca aaattaaatc atgtggttga atgttttttc
      421 cagaaatcaa tctatcccag gattgagcca tgtcaatgtc tggtgcaaac aaccccttcc
      481 cataattcaa ctcatgaaca ttattaaaca catgatcgaa cgctttatca acatcatctt
      541 ttgaaaagcc attcatttct ttgaacttcc aaatgttcga atagatttta cgtttttcca
      601 aatcttgatt gctgtttttt atcctgtcgt aggttaaata agcatgtttt tcggctttaa
      661 taaattcctt tggcaaaagc tcgtcgtttg aatcatcacg aacataattc tttgcgccag
      721 cttgaacaag tagtccgatg cctttccttt cctctatttt agcatttttt aaaggatcag
      781 accaacgttt tgagtgtgca ttctgttttt tgccgtttcc tgggtgataa tcaacagtac
      841 acctgcaccg attatgcctc ctccaaacat cctttggaac atctggatag ttataaaccc
      901 caacaacagc cctacaccat ttacaacaat taccactttc ttttctgaca actttaggtg
      961 tcaaccctgc cttggcatga aaatccgcat tcgttttgat actatcatcc acaattgact
     1021 gggtaaaatt gacaatcggt tctttcagca accacgacac tttttcaaaa tcaacttcag
     1081 atgacaagcg attgaccaag ccatcaactc tatcttgatt gaatttcgga acttggactt
     1141 ttagaccgat attagcttcc ttgttcaagt tcgcttggac ttgtcctgta aagtcgctca
     1201 caagctcaaa attttttcct agcacgtcat tcagtaaacg ttggccgata ttgtaataca
     1261 tttttccatc tggcaaccta tcgcccgcaa cagatgagcc aagagcttct gctaagatgt
     1321 ctccaagctc aatggcaaaa tcatttgctg tggaataggt tgcctttttg tttttgagct
     1381 tggcaaaagc agagctgata atgtcacttt ttccatagga agcttcaaac ttatcctgaa
     1441 cttctttcag taagctaggt aagatatcat cattcatttg ctaccacctc cgctaacttc
     1501 ggagtggccg acatatcacc agcaatacca gtcaaatccc gaatggtttc ggcgttgata
     1561 tagccaggta aggcttgatt aagtttgata gctccatcac caatcatggt cagcatgttg
     1621 gcgtcagctt caaagagcgg ttcccacttg atttttgtat tgagaaactg ctctctggtg
     1681 tattggtatc tatcccgtaa acaaacggcc acataggcta catttaacag accagatgcg
     1741 attgagcgtt gggcttttcg ccctgcagcc ctcaaattct cgtgcgccgc cttgatagct
     1801 tcgacagatg atggattatc cgatggaaaa ccaaggtcat ccatggtcag accgcttcca
     1861 ccggcaaata atgatgcata cattcgcaac tgatcaataa atggtgccat gctagcagtg
     1921 ttaaactgac cgacattagg actatctccg tcgtcgtcct ttgtaatttc aaggagactc
     1981 gacactgtcg ttttccactt ctccatcggg tcagcatctt gactagttcc cagaacatac
     2041 ttttgcggga acgagtaaaa ttctgctgtc acttctgcac gctctagggt tcgcttagct
     2101 gccaactgat tgtacatgcc tgccctagta attcgactgc gaccaaaggg acggaccgca
     2161 tcaggtctgt gaataattgg cactagtaaa ggttgaccgg tcggattgga gatactgtat
     2221 ggatttgcac ctttagggta ataccatgtc acatctttgg taaaatatgc ctctaatacc
     2281 gggttgtcgt tttcgtccac ttctaggatt gcatatccct cagtcagcaa aaatgttgtg
     2341 gtgtcaatga caccggttgc cttgctggct tcaatgacct gcattttagg caagccatca
     2401 ggatttggga ccaagtacac aaaacaacaa gatgcaatca aggctgattg aatagccgtg
     2461 tcaaaaaaga tatctgggtt gttagcatca aagatttcgg ttgcttcaaa atcatcattt
     2521 gcaaactcac ggaaaatgat tctgtcagcc aaggcatcca cactgtgagc cgtccatccc
     2581 ataaccgagt tatacatgct tttgacattg tcagggatga taaaactttt ggtattatct
     2641 ctgtctgcca tagcatagaa cctataacgt ttcttaactc ctaattgata tagagctaac
     2701 ttcttccgga gataactcat tcctttgtaa ctcattttgg ctgctcctta attttattga
     2761 tcacttgctc ttcaaagtct ggttcgtgga cttccacacc ttcgacaatg gtccgactag
     2821 taaaaccaaa ctcagcttca ttggccaaaa tcttggtttt aaaatcttga tagtatttca
     2881 gcaaattttt ctttttttta cgttttttgg ctggttggtt gttaggatta tcttttttag
     2941 atttttgctt tttgaggtta gcgcgttttt gtttcatcaa tttacgctgt ttgtctctag
     3001 ccccgtcctt acggcattta tcactgcaat atttaacccg ttttgattct gccgtgaagc
     3061 ttcgtccaca aaaagcacat gattttttta tcatttttga taactcctat accacttaat
     3121 cctaacgcga aaaaaaatgt acagtgacgg cgtgaagggc cgccgagcga gttggtgggg
     3181 ttctaccccc cttcagattt tacagagata aactgtgacc aatcgcgact ctgaggaaga
     3241 tttctatttc ccaatgtttt ttgttctgct ttagaattat ctgcgaataa cttatcagat
     3301 ttaattctat tgcaagacat gtgagccaac tgcagatttt caattaacga tggatgaccg
     3361 ccttttgcta atggaaccac atgatcgatt actgcacaca ttggatttgg atattttaat
     3421 ttcttatcaa ctaactgacc acatattcca caatgtgttg cagtcttaag tattctcttc
     3481 ttattctttt caaaagctac tctatgcgtt ccgctcttgt ctgctctcat cgttacctca
     3541 aacaaaagag gcaccgtata attacagcac ctctcaattt cttgatgata taataatagc
     3601 acgttgaaac tgtcatgcac tgtcaattac tgtatttttt gcaaatctct gtcatttact
     3661 gtcatttcgt taagctctct tgttgccaca cgtaggatac ggtaataggt ggactcactg
     3721 caattaaggg catccattac ttgccagggg ctcattttat ctatatagac catagagagt
     3781 accgtttgag agtctgaatt actaagacca tcaatcatac tctgtagttc gcg
//
LOCUS       DAZQCJ010000133         3776 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000133 DAZQCJ010000000
VERSION     DAZQCJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.135"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..379
                     /locus_tag="ACHU66_001985"
     CDS             <1..379
                     /locus_tag="ACHU66_001985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecF"
                     /protein_id="HFR3390239.1"
                     /translation="NFAKHENKWRNNSEINDSNIPYLKTSIYSLIANSSLLDELKRVL
                     KEHVANERLFLEFNINSKETSQHLDILYKEVSVLSLGQKVVAMLDFLLAYSDYSKDFR
                     PLIIDQPEDNLDNRYIQTFSSTV"
     gene            384..614
                     /locus_tag="ACHU66_001986"
     CDS             384..614
                     /locus_tag="ACHU66_001986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390240.1"
                     /translation="MKAQRQIILATHNATIVTNSMTDQVVIMESDGAHAWIESQGYVS
                     EKFIKNHIINQLEGGRDSFKHKMSIYETALSE"
     gene            952..1314
                     /locus_tag="ACHU66_001987"
     CDS             952..1314
                     /locus_tag="ACHU66_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1252 family conjugative relaxosome accessory
                     protein"
                     /protein_id="HFR3390241.1"
                     /translation="MTESYRTIRKEINLTLEEFEQIQLLMRKENAEQFSPFVRQKLLD
                     IVNNKDDIRDWFILWQSQKIEQISRDILQVATLAERGHQVTSEHLRIILTCVQELMTE
                     VEKAVPLSSEFRDKYMGG"
     gene            1317..1682
                     /locus_tag="ACHU66_001988"
     CDS             1317..1682
                     /locus_tag="ACHU66_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR3390242.1"
                     /translation="MENRYRTNLKKVFLTDQELANLNNHISQSSCQNFSAYARKVLLN
                     PNMTFLTIDTSNYQDLVFELRRIGNNINQIAKTVNQTKLLTVEQRDNLKTGIGQLIAE
                     VDKDFYVKCQKLREFYGSH"
     gene            1669..3552
                     /locus_tag="ACHU66_001989"
     CDS             1669..3552
                     /locus_tag="ACHU66_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1250 family conjugative relaxase"
                     /protein_id="HFR3390243.1"
                     /translation="MVVTKHFAVHGNKYRKSLIKYILNPAKTDQLKLVSDFGMSNYLD
                     FPNYEEIVEMYQANFVNNDRLYDSRNDRQQIKQNKIHAHHLIQSFSPEDNLTPEEINR
                     IGYETIKELTSGNFRFIVTTHTDRLHVHNHILINSVDLNSHKKLKWDYAQERNLRMIS
                     DRLSKEAGAKIITPNRYSHEKFVTYRKSNHKFELKQRLYFLMENSKDFDDFLSKAEAL
                     NVQIDFSRKYARFLMTDMPMKRVIRGKQLDKRQPYTEEYFREHFAKRAIEQCLDFLLP
                     RVRDLSQLLEFARELNLAMSLKQKNVAFTLTKNSQSITVNNQKVSSKNLYDVQFFETY
                     FEKRGEVPDIDQSQLISDFEGYCEEQDKEKLAYEDLQEAYQAFKEKRDQVQEFEVVLA
                     DHQIDKLVKDGLFIRMNYGVKKDGLVFIPNRHLDIKETESGKHYHAFIRETAQFFIYN
                     KEASELNRYMRGRELIRQLTNDSQTISKRKRPTIDTLKKKIEEINLLIELGTENKSYQ
                     DIKDEIIKDIAQLDLTITEIQEHIDHLNKVAEVLLNLDNNDIENRRLAKYDYAKMNLT
                     AAIKLEQVEKEIEIYQIKLDKSIDDYEYLIRRLEKFVKILNEIDSSKDELKFEHSEKN
                     VKE"
BASE COUNT         1336 a          606 c          743 g         1091 t
ORIGIN      
        1 gaattttgca aaacatgaga ataagtggag aaataattcc gagattaatg attctaacat
       61 accctattta aaaactagta tttattcttt aatcgcaaat agtagtttac tagatgagtt
      121 aaagagagtg ctaaaagaac acgtagccaa tgaaagactt tttttggagt ttaacattaa
      181 ctcaaaagaa acatcacaac atcttgacat tctttataaa gaagttagtg tattaagttt
      241 gggtcaaaaa gttgtagcga tgttagattt tttactagca tatagtgatt attcaaagga
      301 tttcagacca ttaattattg atcaacctga agataatttg gataaccgct atatacagac
      361 atttagttca acagtttaga gacgtgaaag ctcaacgaca aattatttta gcaacccata
      421 atgcgacaat tgtaacaaat tccatgaccg atcaagtggt aattatggaa tcagatgggg
      481 ctcatgcttg gattgaatca cagggatatg ttagtgaaaa atttataaaa aaccatatta
      541 tcaatcaatt ggaaggtgga agagactcat ttaaacataa gatgtccata tatgagacag
      601 ctctttctga ataatataag gaaatgaaaa gtaggttaga aatatcctac tttttgtttt
      661 gtgatacctt ttccaaatca cctctattat cctaatatca ttcctaaaaa aatcaatcca
      721 tgatttttca ctgggggttt gggggcgaag ccaccaagtt atcttatcat cggctatcaa
      781 aactcaaagg ttttggtcgg ctggcgataa gatttttggg atattgtgga cacaatatct
      841 gagctcgcaa aagccgaaaa gggaagattt gaggctttta gggagccccc gtatcagata
      901 tggtatcggt gctaacccgc tagagggaaa tgtgagaagg agtgtatcat catgacagaa
      961 agttatcgga caattcgtaa ggaaatcaac ttgacgcttg aggaatttga gcagattcaa
     1021 ctactgatga gaaaggagaa tgcggagcag ttttctccct ttgttagaca aaaactattg
     1081 gacattgtca ataacaagga tgacatcaga gattggttta ttctgtggca atctcaaaaa
     1141 atagaacaaa tcagtagaga tattttacag gtagcaactc tagcggaacg aggccatcag
     1201 gtcacgtcag aacacttaag aattatattg acttgtgttc aagaactgat gacagaagta
     1261 gagaaggcag taccactcag ttcagaattt cgagataagt atatgggagg ttagatatgg
     1321 aaaatcgtta tcgaaccaat ctcaaaaaag tctttttaac agaccaggaa cttgcgaacc
     1381 tgaacaatca tattagccaa agttcttgtc aaaatttttc tgcttatgcc agaaaggttt
     1441 tgcttaatcc aaatatgacc tttttaacaa ttgataccag taactaccaa gacttggttt
     1501 ttgaattacg tcgaattggc aataacatta accagatagc taaaacagtc aatcagacaa
     1561 aacttctgac agtagagcag cgagataatt tgaaaacggg cataggtcag ctgattgcgg
     1621 aagttgataa ggacttctat gtcaagtgtc aaaagttgag ggaattttat ggtagtcact
     1681 aaacattttg cggttcatgg caacaaatac cgtaaaagtc ttatcaagta tattctaaat
     1741 ccagctaaga ctgaccaact caaattggtt tcagattttg gaatgagcaa ttacctcgac
     1801 ttccctaatt atgaagaaat agttgagatg tatcaggcaa actttgtcaa taatgatagg
     1861 ctttatgatt cgagaaatga ccggcaacaa atcaagcaaa ataaaattca tgctcatcat
     1921 cttatccagt cattctcacc tgaggataat cttacacctg aagaaatcaa tcgtattggt
     1981 tatgagacga taaaggagtt gactagcgga aactttcgct ttattgtgac aacccatacg
     2041 gataggttgc atgttcataa ccatatcctc atcaattctg ttgacctcaa ttctcataag
     2101 aaactcaaat gggattatgc tcaggagaga aatttacgga tgatttctga ccgtctttcc
     2161 aaagaagcag gtgccaaaat tatcacaccg aaccgttatt ctcatgagaa gtttgtgacc
     2221 tatcgcaaaa gtaatcacaa atttgaattg aagcaacgtc tttattttct gatggagaat
     2281 agtaaggatt ttgacgactt tttatccaag gcagaagcat tgaatgttca gattgatttt
     2341 tcaagaaaat acgctcgttt tttgatgacg gatatgccca tgaagcgagt tattcgaggc
     2401 aagcaactag ataaacgtca accctatacc gaggaatact ttcgagagca ctttgccaag
     2461 cgagcgattg agcaatgttt agattttctc ttacccagag ttcgtgacct ttcacaactt
     2521 ttggagtttg caagagaact caaccttgct atgtctctaa aacaaaagaa tgtggcgttt
     2581 actctaacaa aaaatagtca gagcattaca gtcaataatc aaaaagtcag ttccaaaaat
     2641 ctctacgatg ttcaattttt cgaaacttac tttgagaaac gtggagaagt tcctgatatt
     2701 gaccagtcgc aacttatttc cgactttgag ggatattgtg aggaacaaga taaggaaaag
     2761 ctagcttatg aagacttgca agaggcttat caggcattta aggagaaaag agaccaggtt
     2821 caagagtttg aggtcgtctt agctgaccat cagattgata agttagtcaa agatggtctc
     2881 tttatcagaa tgaactacgg cgttaaaaaa gatggcttgg tctttatccc aaaccgtcat
     2941 ttagatatta aagagacaga gagtggtaaa cactaccatg cctttatccg tgagaccgca
     3001 caatttttca tttacaataa ggaggcgtcc gaactcaatc gctatatgcg agggcgtgaa
     3061 ctcattcgtc agttaaccaa tgatagccag actatctcta aaagaaaaag accgaccatt
     3121 gacacactta agaaaaagat tgaagagatt aatcttctta ttgagttggg tacggagaat
     3181 aagtcttatc aagatattaa agacgagatt attaaggata ttgctcaatt agatttgacc
     3241 attacagaga ttcaggaaca catagatcat ctcaacaagg ttgcggaagt cttgcttaac
     3301 ctggacaata acgatataga gaaccgccgc ttggccaaat atgactatgc caagatgaat
     3361 ctaactgcgg ctataaaact ggaacaagtt gagaaagaaa tcgaaatcta tcaaattaag
     3421 ttagataagt caatagatga ttatgagtat ttaataagga ggttggaaaa gtttgtcaaa
     3481 attttaaatg aaatagactc aagcaaggat gaactaaaat ttgaacacag tgaaaaaaat
     3541 gtgaaagaat agcatttttc ggatttattg ataccaaata ctgagtactc tgaagaaatt
     3601 ctgaaataaa attgataaaa aggaataaaa aactaaggag atgatattat gaaaaagata
     3661 ggcttattgt cgaatgtttt tatatcattg tttattgtta tattagctag cggttctgtg
     3721 tcatcaggag taagtgctaa tgaaaataag gaaattagtt ataatgtttt agttag
//
LOCUS       DAZQCJ010000134         3702 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000134 DAZQCJ010000000
VERSION     DAZQCJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.136"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            305..745
                     /locus_tag="ACHU66_001990"
     CDS             305..745
                     /locus_tag="ACHU66_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390244.1"
                     /translation="MTENDYLEQTEKDRLELEQHRLNYMADDTPIEPSDIPKLMEIAK
                     KLQAEDTSLNIYELYKHPEARAKLFSQITEACYMALNATPTQAQRLAFCDYLEQQYEN
                     TLKKMVASTDKQALGELLDLLELPVEIESQFIRDMAISGLLAKG"
     gene            815..1315
                     /locus_tag="ACHU66_001991"
     CDS             815..1315
                     /locus_tag="ACHU66_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390245.1"
                     /translation="MIELTIESITKPLKQKRLSRVSGKLKGVGITIDLNNLTVNYGSQ
                     QINLASIPGTYGGVRYFFLCPICEKRCRKLFKNSLIFACGTCQDRHRATLNRSKTDCQ
                     YYFELAFKEARKIDPSYYPEKGYVDYDNFPSRPKRMRAKTYWKHYRKFTSYLDKGVSN
                     WLGGLK"
     gene            1455..1841
                     /locus_tag="ACHU66_001992"
     CDS             1455..1841
                     /locus_tag="ACHU66_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFR3390246.1"
                     /translation="MFEINMTINERLRDIRDLKDAISSLENDKLELEKTYPVQSRRIR
                     KKKARLLVAIRGIKVKRQRMTDLINQLSDENQRKILTLQYIEGVKDKHLVEVSGLKDY
                     REVSSIRQKAIKNLERLQKQLEQPQA"
     gene            2025..2207
                     /locus_tag="ACHU66_001993"
     CDS             2025..2207
                     /locus_tag="ACHU66_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002961998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR3390247.1"
                     /translation="MPMTQKEMVKLLTAHGWTKTKGGKGSHVKLEKVGERPITIPHGE
                     INKYTERGIKKQAGLL"
     gene            2247..2699
                     /locus_tag="ACHU66_001994"
     CDS             2247..2699
                     /locus_tag="ACHU66_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR3390248.1"
                     /translation="MLVTYPALFYYDDTDGATAPYFVTFPDFEHSATQGEDMADAMAM
                     ASDWLGIHLADYIENGRDIPTPTPINALSLADNNPFRDDKDIELVYDPSKSFVSMVMV
                     DVAEYLGSQEPVKKTLTIPRWADTLGRELGLNFSQTLTDAIADKKIHA"
     gene            2811..3020
                     /locus_tag="ACHU66_001995"
     CDS             2811..3020
                     /locus_tag="ACHU66_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390249.1"
                     /translation="MAKPKEKWTPIITHIVPKDTELADGTILDKETTLSQEEFSKNPP
                     VIPDGHPFYNIWARIIREKIEKGEL"
BASE COUNT         1294 a          650 c          721 g         1037 t
ORIGIN      
        1 tatgctttcc atcgccaaac aaaacattaa gcatagggta cttggtataa aaaactaatt
       61 gaaaatccct ttatttttta taaatgtgac cgtgtgacca tcttttttat ttattatata
      121 ggagttggaa atcatttcat cttttctata taaataattt ttcaaatctt ggtaacataa
      181 aaacgacagc ctctaaaccc ttgataatac taggttaaaa cgtgttacca agatttttta
      241 aagttggtaa catttgccga aattttacat aatttttctt aaagtaagaa atggagacta
      301 aaaaatgaca gaaaacgact acctagaaca gacggaaaaa gacagattag aacttgagca
      361 acaccgccta aattatatgg ctgatgatac acctattgag cctagcgata ttcctaaact
      421 aatggaaata gctaaaaagt tacaagcaga ggataccagt ctgaacattt atgaactgta
      481 taagcaccca gaagcgcgtg ctaaactatt ctcacagata actgaagctt gttatatggc
      541 tttgaatgct acaccgacac aagcccaaag attggcattt tgcgactatc tagaacagca
      601 atacgaaaac accttaaaga aaatggttgc cagcacagac aaacaggcac taggtgagtt
      661 actagatttg ttagagctac cagtagagat agaaagccag tttatccgag atatggcaat
      721 cagtgggtta ctagccaaag gctgaacagg gaggcacgcg cctcttttgt tgtttaatca
      781 ataaatttgg gttttgccaa taagggaggg gaaaatgatt gaattgacca tagaaagcat
      841 aaccaaacca ttgaaacaaa aacgcttatc aagagtatcc ggtaagttaa agggagtagg
      901 tatcactatt gaccttaata acctaactgt taactacggt agccaacaga taaacctagc
      961 tagcatacca ggcacttatg gaggtgtccg ctacttcttt ctttgtccta tttgtgaaaa
     1021 gaggtgtaga aaactattca aaaatagcct tatctttgct tgcggtactt gtcaagatag
     1081 gcatagggca actttgaacc gaagtaaaac agactgccag tattattttg aactagcttt
     1141 caaagaggct aggaagatag accccagtta ttacccagaa aaagggtacg ttgattatga
     1201 taattttcct agcagaccaa aaagaatgag ggctaagaca tactggaaac attacagaaa
     1261 gtttactagc tatttagata aaggggtgag caattggttg ggtggactaa aataacaggg
     1321 tgcatttaca aaacaaacaa ataaagaacg ctctattagt tcccaaaaca aataaactta
     1381 ctcaatcgaa caaagaacga acaaagaata cgcgcttttt tgatataatc atagtcatag
     1441 cgaggtatta aattatgttt gaaatcaata tgacgattaa cgagcgacta agagacatta
     1501 gagatttgaa agatgctatt tctagtttag aaaatgataa acttgaactt gagaaaactt
     1561 atccagtaca gtctaggaga attagaaaga aaaaagcaag attattggta gcaatacgag
     1621 gaattaaagt aaaacggcaa cgtatgacag atttaatcaa tcagctatca gacgaaaacc
     1681 aacgtaaaat tttgacctta caatatatcg aaggagtaaa agacaaacat ctagtagagg
     1741 taagcggtct aaaagattat agagaagtct ctagcattag acaaaaagct attaagaact
     1801 tagagcggtt acaaaagcaa ctagaacagc cccaagctta aatttagccc tattttagcg
     1861 cgtgttttgc tttatccgat aaattaacca tacagcaact aaaagccctt agaaacgatt
     1921 ttaggggcta tttttgataa ctgatattag acaaaatatt atctttttac ttgaatacgt
     1981 gtagtacgtg tgatataatg atgttgtcag gaggtaaaag cgctatgcct atgacacaaa
     2041 aagaaatggt taagctactt accgcccatg gttggacaaa aactaaaggt ggtaaggggt
     2101 ctcacgttaa acttgaaaaa gtaggcgagc gacctataac cattccccac ggtgaaatta
     2161 acaaatacac cgaaaggggt atcaaaaagc aagcagggct gttataaaag cagtccctcg
     2221 cttgtctttg attttggagg taaactatgc ttgtcactta tccagcttta ttttattatg
     2281 acgatacaga cggagctacc gccccttatt ttgtcacttt cccagacttt gaacattcag
     2341 caactcaggg cgaagatatg gcagacgcta tggcaatggc tagtgattgg ttaggtattc
     2401 atctagctga ttatattgag aatgggcgtg atattcccac tcctacccct attaacgctt
     2461 tatcattggc tgataataac cctttccgtg atgataagga catagagcta gtttatgacc
     2521 ctagcaagtc ttttgtatct atggttatgg ttgacgttgc tgagtatcta ggcagtcaag
     2581 agcctgtaaa gaaaacacta actattccac gttgggcgga tactttaggg cgtgagttag
     2641 ggctaaattt ctcacagacc ttaacagacg ctattgcaga taagaaaatt catgcttaac
     2701 actatctaac ttatatgata gggctaaacc agtacactaa aacaggtaac tgctaaggtg
     2761 taaggttagc cctattttgt atttttacac aataaaggag taattttaac atggctaaac
     2821 ctaaagaaaa atggacacca ataataaccc acattgtccc taaagatact gaactagcag
     2881 acggcacaat attagacaaa gaaaccacct tatcacaaga agaattttct aaaaatcccc
     2941 ctgttattcc agacggacat cctttttata atatttgggc taggataatt agagagaaga
     3001 tagaaaaggg tgagctatag caccaagata cagtagaaaa cagtaaaata cagtagattt
     3061 gcgcgtgata attgcttgaa attgatttgt caaggtcaac aaaattgata accacgcgca
     3121 tttaagctac ttaatttacg gttgataaaa tcccaatacg gtaaagtttt aaccttttcc
     3181 aatttggaaa cagttatcta tcttttaaaa atggcatatt gtgagaaata actaacttgt
     3241 gccagctatc cgtggtataa tatattcaca gctggaggct tgcatagtct agccgattag
     3301 ggagagttaa cccgtgaggt catacactcc cttttttggt ataaaatagc tatcagcaag
     3361 acatcagata actaaaaagc accttatcaa gtgtacttaa gatttgcttt tgtaaagtga
     3421 atatgatagc atgaaaatat agtatccttt acaggttcca ccgctattct gatagcaaag
     3481 gagcggaata tagtgcgaca ttgaatgccg ctcagtgata aacgtaccag ccaatatgtc
     3541 gttagttttg ctggtgtccc ctgttggtgg ttgaggggat agtgaggagg ggtatccctc
     3601 ttttttgtat ggtaattaag aagacctgaa aaaagcacct tgtgggggtg ctagttcttg
     3661 cctgctgaac tcatcgtttt aatacctttt ttactacccc tc
//
LOCUS       DAZQCJ010000135         3677 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000135 DAZQCJ010000000
VERSION     DAZQCJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.137"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(3..911)
                     /locus_tag="ACHU66_001996"
     CDS             complement(3..911)
                     /locus_tag="ACHU66_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR3390250.1"
                     /translation="MAEKRFGILITLIAGIAWGLSGVSGQYLMSRGVSVDMLTSLRLL
                     VSGFFLVGLAYATAKEQLLIVLKNRKALLGIFVFAMLGLVLNQMAYLQAIYHTNAGTA
                     TVLQYLCPILVLAYTCLKNREKPSGIELISIILAVAGTFLIATHGKLDELAVTPIGLF
                     WGIFSAFTYALYIILPGKLIRQYGSITVIGLGMLMGGFVVTLGLQTWQHRLPIDGGSF
                     LGLLGIVGVGTIFAYTAFLKGVSMVGPVNGSLLASIEPIASVFFAVWLVNEQFYAIDF
                     VGMLFILLAVLLISLKDILVAQKSIG"
     gene            complement(986..2467)
                     /gene="galT"
                     /locus_tag="ACHU66_001997"
     CDS             complement(986..2467)
                     /gene="galT"
                     /locus_tag="ACHU66_001997"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFR3390251.1"
                     /translation="MMAGLVDRFVEQVIANSDFEEMDALYLHNRVLALVGDQVLTVQT
                     DLEDLIELKDELLAHGVRTGFVGELLEEQDMVGACLMDLMTPSPSQVNRDFWQTYQDS
                     PEQAIGDFYELSKRNDYIKMAAIAKNIYYQVSTEYGDLEITINLSKPEKDPKSIAAAT
                     KAEASNYPKCLLCMENEGYQGRINHPARANHRIIRLELGQEKWGFQYSPYAYYNEHAI
                     FLNQEHVPMVISPRTFEQLLDLLDLLPGYFVGSNSDLPISGGSILTHNHYQGGRHSFA
                     MEKAPIERQLVFDGFESVSAGIVKWPMSVIRLSSADKPSLLGLATKILEKWRSYSDDN
                     VQIKAETDGTPHHTITPIARKRGDLYELDLVLRDNQTSEEFPDGIYHPHPDVQHIKKE
                     NIGLIEVMGLAILPPRLKAELAEVEKFLLGQDSQVADYHQPWAESLKTAHPDVTEETV
                     EQVVRESVGQIFARVLEDAGVYKRTPEGQAAFLRFVEFVGLAT"
     gene            complement(2477..3649)
                     /locus_tag="ACHU66_001998"
     CDS             complement(2477..3649)
                     /locus_tag="ACHU66_001998"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR3390252.1"
                     /translation="MNTEQLKQAFLDVFGQEADATFFSPGRINLIGEHTDYNGGHVFP
                     AAITLGTYGAARKRDDQLLRFYSANFEELGIIEVDLNNLVFDKADNWTNYAKGVLKFL
                     QEAGHAIDTGMEVFVYGNIPNGSGLSSSASLELLIGIIAEELYGLELTRLDLVKIGKQ
                     TENHFIGVNSGIMDQFAIGMGADNRAIYLDTNTLEYDLVPLDLGDHVIVIMNTNKRRE
                     LADSKYNERRAECEKAVEELNAVLNIQTLGELDEWTFDQYSYLIKDENRIKRARHAVL
                     ENQRTLQARKALEEGDLATFGRLVNASHVSLEHDYEVTGLELDTLAHTAWEQEGVLGA
                     RMTGAGFGGCGIAIVHKDKVEAFTENVGKTYTEVVGYEPSFYVAEIAGGSRVLSRK"
BASE COUNT         1097 a          947 c          672 g          961 t
ORIGIN      
        1 tattaaccaa tactcttttg ggcaaccaat atatctttta aagaaataag caaaacagca
       61 agtaaaataa acaacattcc aacaaaatca attgcataaa attgttcatt gaccaaccat
      121 actgcaaaaa agacagatgc aatcggctca atcgaggcca aaagactgcc attgacagga
      181 ccaaccatac tgacgccttt aagaaaggca gtataggcaa aaatcgtccc gactccgaca
      241 atacctaaca ggcccaggaa acttccacca tcaataggaa gacgatgctg ccaagtttgc
      301 aaaccaagcg tgactacaaa accacccatt aacatcccta aaccaatcac tgttatactg
      361 ccgtattgac ggatcaattt acctggcaaa ataatatata gcgcataagt aaaggctgaa
      421 aagattcccc agaacaaacc gattggagtc actgccaatt catctaattt tccatgagtc
      481 gcaatcaaaa aagttcctgc tacggctaag ataattgaaa tcaactcaat ccctgatggt
      541 ttttccctat ttttcaaaca ggtataagcc aagactaaga tagggcaaag gtattgtaaa
      601 accgtagccg ttccagcatt ggtatgatag atggcttgta aataagccat ctggttaaga
      661 accagaccca acatcgcaaa aacaaaaatt cccaacaagg cttttctatt tttcaacact
      721 atcaacagct gttctttcgc tgtagcataa gccaggccaa cgagaaagaa tcccgatacc
      781 aacaaacgca gggaggtaag catatccact gaaacaccac gtgacatcaa gtactgtcca
      841 ctcactccag acaagcccca ggctatacct gctattaatg taatcagtat cccaaatctc
      901 ttttccgcca ttatatctcc ttctcacact gtgaatactt tcattatacc aaaagagcag
      961 gacaccgtcc cactctttct tgcaattatg ttgctaaacc tacaaattct acgaatcgta
     1021 gaaaggctgc ctgtccttct ggtgttcgtt tgtagacccc tgcatcttcc aatacacgcg
     1081 caaaaatttg tcccactgac tcacgaacaa cttgttcgac agtttcctct gtcacatctg
     1141 ggtgagctgt tttcaggctt tccgcccaag gctgatgata gtcagccacc tgactatctt
     1201 gacccagtaa gaatttttct acttcagcca attcggcctt caagcgtgga ggtaaaatcg
     1261 ccaagcccat tacctcaatc aagccgatgt tttctttctt tatatgttgc acatctggat
     1321 gtggatggta gatgccatct ggaaactctt ctgaggtctg attgtcgcgg agaaccaaat
     1381 ccagctcgta caaatctcct cgtttccgag caatcggggt gatggtatga tgaggagttc
     1441 catccgtctc agcctttatc tgaacgttat catctgagta gctccgccat ttttccaaaa
     1501 tcttagtcgc taaaccaaga agcgatggct tatctgccga actcaagcga atcaccgaca
     1561 taggccattt gacaattcct gccgaaacgg actcaaagcc gtcgaagacc agctggcgct
     1621 caatcggcgc cttttccatg gcaaaactgt gccgcccacc ctgatagtga ttgtgggtca
     1681 agatggagcc acccgagata ggtaaatcag agttggaacc aacaaaatag cctggcaaca
     1741 aatccaacaa gtccaagagc tgctcaaatg ttcgcggact aatgaccatc ggcacatgtt
     1801 cctgatttag gaaaatagca tgttcattgt agtaagcata gggcgaatac tggaagcccc
     1861 acttttcctg tccaagctcc aagcgaataa tgcggtggtt ggcgcgtgct ggatggttaa
     1921 tccgaccctg atagccctca ttttccatac agagtaggca tttgggataa ttgcttgctt
     1981 cagctttcgt agcagctgca atagacttgg gatccttctc aggtttagac agattgattg
     2041 tgatttccaa atccccatac tctgtcgaaa cttgataata gatatttttc gcaatcgcag
     2101 ccatcttgat atagtcattg cgcttgctca attcatagaa gtcaccaatc gcttgctctg
     2161 gactgtcctg ataggtctgc caaaaatcac gattgacctg acttggactt ggtgtcatca
     2221 aatccatcaa acaagcccca accatgtcct gctcttccag caattcaccc acaaaaccag
     2281 ttcgaactcc atgagccaat agttcatctt ttaattcaat caagtcctct aaatctgttt
     2341 gaacagtcag aacctggtct cccaccaaag ccaagacccg attgtgcagg taaagagcat
     2401 ccatttcctc aaaatcactg ttggcaataa cctgctcaac aaaacgatcc accaatccag
     2461 ccatcatttc tccaatctat tttcgagata aaacgcgaga gcctccagca atctccgcta
     2521 catagaagct tggttcatag ccaaccactt cagtataggt cttgccgaca ttttccgtga
     2581 aggcttcaac cttatccttg tggacaatgg caataccaca gccgccgaag ccagctcctg
     2641 tcatccgagc accaagaact ccttcttgct cccaagctgt gtgagccaag gtatccaatt
     2701 ctagacctgt cacttcataa tcatgctcca aagaaacatg ggaagcattg accagacgac
     2761 caaaggtagc caaatctcct tcttctaaag ccttacgagc ttgcaaggtc cgttgatttt
     2821 ctaagacagc atggcgagca cgcttgatac ggttctcatc tttaatcaaa taactatatt
     2881 ggtcaaaggt ccactcatcc aattcaccta gagtttgaat attcaagact gcattcaatt
     2941 cttccacagc tttttcacat tctgcacgac gctcattgta cttagaatct gccaattcac
     3001 ggcgtttatt ggtgttcata atgacaatca catggtcccc taaatccagc ggtaccaagt
     3061 catattccaa ggtattggta tcaaggtaaa tcgcccgatt atctgctccc ataccgatcg
     3121 caaactggtc catgatccca gaattgacac cgataaagtg attttccgtt tgtttcccaa
     3181 ttttcaccaa atcaagtcga gtcaattcaa gtccatacaa ctcttctgcg ataatgccaa
     3241 tcaagagttc caaggaagct gatgatgaca agcctgaacc atttggaatg ttaccgtaaa
     3301 caaagacttc catccctgta tcaatggcat gccctgcttc ttgcaagaat ttgagaacac
     3361 ccttggcata gttggtccag ttatctgcct tatcaaagac cagattgttc aaatccactt
     3421 cgataattcc aagttcttca aagttagcag aataaaaacg caaaagctgg tcgtcacgtt
     3481 tgcgagcagc cccgtaggtc cccaaggtaa tagccgcagg gaaaacatga ccaccattgt
     3541 agtccgtgtg ctcaccaatc aaattaatcc gacctggtga gaagaaagtc gcatctgcct
     3601 cttgaccaaa cacatcgaga aaggcctgct tgagttgttc tgtgttcata agaacctcct
     3661 agattttgta atcgttt
//
LOCUS       DAZQCJ010000136         3648 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000136 DAZQCJ010000000
VERSION     DAZQCJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.138"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(251..844)
                     /locus_tag="ACHU66_001999"
     CDS             complement(251..844)
                     /locus_tag="ACHU66_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFR3390253.1"
                     /translation="MTQIKWRDCLLICFGAILYALVLNMLIIPHEFGEGGITGVTLLL
                     FYTTGIETSLSSFVLNGILIVLAYRFLSKLTVFYTFLAVATMSLTMHYTTFLQIKWLP
                     LIPSALLAGLVTGLSMALVIYGNGSTAGSDIIALLCNKYFKWKISTVILIFDVFVVTP
                     LAFKIGLVKTFWTLVMVVIISKSLDWFLQALKKNNHK"
     gene            complement(922..1305)
                     /locus_tag="ACHU66_002000"
     CDS             complement(922..1305)
                     /locus_tag="ACHU66_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HFR3390254.1"
                     /translation="MGLFSGLLGNASQMDNDKIEQELEHVLLDNEQVEMAFSLVRDLI
                     VFTEYRLILVDKQGMTGKKVSYKSIPYRSISRFTVETSGHFDLDAELKIWISSAVEPA
                     EILQFKSDKSVIAIQKALASAVLGK"
     gene            complement(1393..>3648)
                     /locus_tag="ACHU66_002001"
     CDS             complement(1393..>3648)
                     /locus_tag="ACHU66_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HFR3390255.1"
                     /translation="IKVNEQYFYYPKDKDANVISISEAKKLTKNQKKPTSQAKKGVAG
                     VDYPTDDGFLFENEGQILSKTDEGLVLEHNGHSHFIFYKDLKESKWSYLVPKEYIENS
                     QSSSHSNAKQAKLSQTFDDGYVFDPKDIVAEDANGYTVRHGDHYHYIWKSSLQYGQGN
                     TGETIGKKGQITVLDNHSLHTPTTRNNFVPLKSTTEKGHTSTQSNSKKAFPGIDYPTS
                     DGFIFDGSGVQGQTALGLLIGHGTHTHLLPYSHLIGSPWESYIPTQYLETARAEYHSS
                     TSTQVDVEIPLSPQEGVKPNSSVEEEREAKKSYLAENMHVSKEKIKVIETDAGPAFVY
                     PHGDHSHTILIEKVEVGKPIEDPHGDPHAHDKIGMATLKQLGFDDEIIEDILHATADT
                     PFPSNETDSEKMREWLKTVKYLNIGQRKDPLKRAGLDLMPNVEVLGIGFTPIDDVKPI
                     LQFKKLKQLWMTSTGVKDYQFLKEIPTLEGIDLSQNGVSDLGFLEEFPNLKVVSAAGN
                     DIEDITILAKLKALESLNLDHNKVTDLSPLADLSQLTAVSLDNNRITDLSALQNKKKL
                     TRLYISQNPQLDISTLKTENLEELTANESNVKDLQFVKNNPNLTSLTLKNNKITELQG
                     IEENEKLVNLDVEGNQIKTLEIEGKQESVVRLNVADNQLKNLEGINDYKALEDLNASK
                     NDIETLAITEPNKTLKTIDVSENHIPKEELNLNDQKIPSAIAEHFPAVEGGSIENNQP
                     KEVDKEAKVGE"
BASE COUNT         1094 a          743 c          577 g         1234 t
ORIGIN      
        1 tatagacatt tgaattaact ttgatacatc gatactttcg gtttgcccta ctctagtacc
       61 agctccttgt ctgaaagtta tactcttcga aaatcaaaat cagacgttgt tgacttgatc
      121 tgatgaactt cagttctatc tgcatctgcg tcgcttagtc tgtttttgat tttcattgag
      181 tattaattca ttcgactata agaaaagaga aaactagtta tacatgcaca actgggtttc
      241 tcttgtattt ttatttatga ttattctttt tcaatgcttg caagaaccag tccaaacttt
      301 tactgataat gacaaccatt accaatgtcc aaaatgtttt tacaagccca attttaaaag
      361 ccaaaggagt taccacaaat acatcaaaaa tgagaataac ggtggaaatc ttccatttga
      421 aatatttatt acacaacaat gcaataatat cacttcctgc cgtggacccg tttccataaa
      481 tgactagagc cattgataaa cctgtcacca atcccgccaa aagagcagaa ggaatcaggg
      541 gcaaccattt tatttgcaga aaagtcgtat aatgcatggt taaggacata gtagcaaccg
      601 ctaagaaagt atagaaaacc gttaacttac tcaaaaagcg ataggctaat acaatcaaaa
      661 tcccattcaa aacaaaactg gacagacttg tttcaatccc tgtcgtataa aacaaaagta
      721 gggtaacacc agtaatccca ccttcgccaa attcatgtgg aatgatgagc atattcaaaa
      781 caagggcata taggattgcc ccaaaacata tcaataagca gtctctccat tttatctgcg
      841 tcatattctc tcctcacaaa cagtccacct tagtatatca aaaaaagcag aaagcggata
      901 gccactttct gtactttttt cctattttcc taagacagct gaagccagtg ctttttgaat
      961 ggcaatgaca ctcttatcgc tcttaaactg aagaatttca gcaggttcaa cagccgatga
     1021 aatccatatt ttcagctcag catctaagtc aaaatgacca gaggtttcta ccgtaaaacg
     1081 tgaaatcgag cggtaaggaa tcgatttata agataccttt ttcccagtca ttccttgctt
     1141 atcgaccaaa atcaatcgat attctgtaaa aacaatcaga tcacgaacca gactaaatgc
     1201 catctcaacc tgttcattat ccagtagaac atgctccaat tcctgttcaa tcttatcatt
     1261 atccatttgc gaggcattac ctagtaaacc tgaaaatagt cccataatat gactcctttg
     1321 gtatttttct atagtataac acaaaaagct actagttgac tatcttaggt aggtctttac
     1381 caaacatgag aattactcgc ctacctttgc ttccttatca acttctttag gttgattgtt
     1441 ctcgatactg ccaccttcaa ctgctggaaa atgttctgca atcgcagatg ggatcttctg
     1501 atcatttaga tttaattctt ccttcggaat gtgattttca ctcacatcaa ttgttttcaa
     1561 agtcttattt ggctccgtga tggcaagtgt ttcaatatca ttttttgaag cattcaggtc
     1621 ttctaaggct ttgtaatcat tgattccttc taaattcttc aattgattat ctgcaacatt
     1681 taatcgaaca accgattctt gtttaccctc tatttccaaa gtcttaattt gattcccctc
     1741 tacatccaga tttactaatt tttcattttc ttctatccct tgtaattcag taatcttatt
     1801 attcttaaga gtaagactag ttaaatttgg attgtttttt acaaactgaa gatcttttac
     1861 attactttca ttagctgtca gttcttctaa attctctgtt tttagagttg atatatccaa
     1921 ttgaggattc tgtgatatat acaaacgggt taacttcttc ttgttttgaa gcgcagataa
     1981 atctgtgata cgattattat ctaaactaac agctgtcaat tgtgacaaat ctgctaatgg
     2041 ggataaatcc gtcactttat tatgatctaa atttaaggat tccaaagctt ttaatttagc
     2101 tagaatagtg atatcttcaa tgtcatttcc tgcagcagaa actactttta agtttggaaa
     2161 ttcttccaaa aagcccaaat cagaaacacc attttgtgat aagtcaattc cctctaatgt
     2221 tggaatttct ttcaaaaact gataatcctt aacgcctgtg ctagtcatcc ataattgttt
     2281 caatttttta aattgcaaga taggttttac atcatcaatt ggcgtaaaac caatccctaa
     2341 cacttcaaca tttggcatca agtccagacc ggctctcttt agcggatctt ttcgctgacc
     2401 aatatttaga tattttaccg tttttaacca ctctctcatc ttctcggaat ctgtttcatt
     2461 agatgggaaa ggagtatcgg ccgtagcatg caaaatatct tcaataatct catcatcaaa
     2521 acctaattgt tttaaagtag ccattccaat tttatcatga gcatgtgggt caccgtgagg
     2581 gtcttcgatt ggttttccaa cttcaacttt ctcaatcaag attgtatgag aatggtcacc
     2641 atgagggtaa acgaaagcag gtcctgcatc tgtttctatc accttgattt tttcttttga
     2701 aacatgcatg ttttcggcta ggtagctctt tttagcttct cgttcttctt ctacacttga
     2761 gttaggtttc acaccttctt gtggtgacaa cggaatttct acatcaactt gagtggaagt
     2821 tgacgaatga tattctgctc tagcagtctc taaatactga gtaggaatat aagactccca
     2881 cggacttcct atcaaatgac tgtacggaag taaatgagta tgcgtaccat ggccaatgag
     2941 taaccctaac gctgtttgac cttgaactcc actaccatca aaaatgaaac cgtcgcttgt
     3001 tggataatca atacctggga aagctttttt tgaattagac tgagtggaag tatgcccttt
     3061 ttctgtggta gacttgagtg ggacgaaatt atttcttgtg gtcggagtat gcaacgaatg
     3121 attatcaaga acagtaatct gtcccttttt tccaatggtc tccccagtat ttccttgtcc
     3181 gtattgcaaa cttgatttcc aaatataatg ataatgatct ccatgacgta ccgtatagcc
     3241 attcgcatct tcagccacta tatccttcgg atcaaataca taaccatcat caaaagtttg
     3301 tgatagcttc gcttgctttg cgttggaatg cgagctggat tggctattct caatgtactc
     3361 tttgggtacc agataggacc atttgctttc ttttaaatcc ttatagaaaa taaaatgtga
     3421 atgaccatta tgttctaaga ccagtccctc atccgttttt gaaagaattt gaccttcatt
     3481 ttcaaaaaga aatccatcat ccgtaggata atcaacacca gctacccctt ttttagcttg
     3541 agaagtaggt ttcttttgat ttttggtgag tttcttagct tcagaaatag aaattacatt
     3601 agcatcttta tccttgggat agtagaagta ctgctcattt acttttat
//
LOCUS       DAZQCJ010000137         3629 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000137 DAZQCJ010000000
VERSION     DAZQCJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.139"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..1114
                     /locus_tag="ACHU66_002002"
     CDS             <1..1114
                     /locus_tag="ACHU66_002002"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFR3390256.1"
                     /translation="LENNFALSRRGAVYFTLINFVLVYLFNVIIKQFKDSFLFSTTYQ
                     KKTILITTAELWENMQVLFESDILFQKNLVALVILGTEIDKINLPLPLYYSVEEAIEF
                     STREVVDYVFINLPSEYFDLKQLVSDFELLGIDVGVDINSFGFTVLKNKKIQMLGDHS
                     IVTFSTNFYKPSHILMKRLLDILGAVVGLIICGIVSILLIPIIRRDGGPAIFAQKRVG
                     QNGRIFTFYKFRSMFVDAEVRKKELMAQNQMQGGMFKMDNDPRITPIGHFIRKTSLDE
                     LPQFYNVLIGDMSLVGTRPPTVDEFEKYTPSQKRRLSFKPGITGLWQVSGRSDITDFN
                     EVVRLDLTYIDNWTIWSDIKILLKTVKVVLLREGSK"
     gene            1153..2322
                     /gene="cps2T"
                     /locus_tag="ACHU66_002003"
                     /gene_synonym="wchF"
     CDS             1153..2322
                     /gene="cps2T"
                     /locus_tag="ACHU66_002003"
                     /gene_synonym="wchF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta 1-4 rhamnosyltransferase Cps2T"
                     /protein_id="HFR3390257.1"
                     /translation="MRTVYIIGSKGIPAKYGGFETFVEKLTEYQKDKSINYFVACTRE
                     NSAKSGITGEVFEHNGATCFNIDVPNIGSAKAILYDIMALKKSIEIAKDRNDTSPIFY
                     ILACRIGPFIYLFKKQIESIGGQLFVNPDGHEWLREKWSYPVRQYWKFSESLMLKYAD
                     LLICDSKNIEKYIHEDYRKYAPETSYIAYGTDLDKSRLSPTDSVVREWYKEKEISEND
                     YYLVVGRFVPENNYEVMIREFMKSYSRKDFVLITNVEHNSFYEKLKKETGFDKDKRIK
                     FVGTVYNQELLKYIRENAFAYFHGHEVGGTNPSLLEALSSTKLNLLLDVGFNREVGEE
                     GAKYWNKDNLHRVIDSCEQLSQEQINDMDSLSTKQVKERFSWDFIVDEYEKLFKG"
     gene            2326..3483
                     /locus_tag="ACHU66_002004"
     CDS             2326..3483
                     /locus_tag="ACHU66_002004"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR3390258.1"
                     /translation="MKKILYLHAGAELYGADKVLLELIKGLDKNEFEAHVILPNDGVL
                     VPALREVGAQVEVINYPILRRKYFNPKGIFDYFISYHHYSKQIAQYAIENKVDIIHNN
                     TTAVLEGIYLKRKLKLPLLWHVHEIIVKPKFISDSINFLMGRFADKIVTVSQAVANHI
                     KQSPHIKDDQISVIYNGVDNKVYYQSDARSVRERFDIDEEALVIGMVGRVNAWKGQGD
                     FLEAVAPILEQNPKAIAFIAGSAFEGEEWRVVELEKKISQLKVSSQVRRIDYYANTTE
                     LYNMFDIFVLPSTNPDPLPTVVLEAMACGKPVVGYRHGGVCEMVKEGVNGFLVTPNSP
                     LNLSKVILQLSENINLRKKIGNNSIERQKEHFSLKSYVKNFSKVYTSLKVY"
BASE COUNT         1238 a          521 c          716 g         1154 t
ORIGIN      
        1 gttagagaat aatttcgcac tttcaagacg tggtgccgtg tatttcacat taataaactt
       61 cgttttggta tacctattta acgtaattat taagcagttt aaggatagct ttctattttc
      121 gacaacctat caaaaaaaga cgattctaat tacaacggct gaactatggg aaaatatgca
      181 agttttattt gaatcagata tactatttca aaaaaatctt gttgcattgg taattttagg
      241 tacagaaata gataaaatta atttaccatt accgctctat tattctgttg aagaagctat
      301 agagttttca acaagggaag tggtcgacta cgtctttata aatttaccaa gtgaatattt
      361 tgacttaaag caattagttt cagactttga gttgttaggt attgatgtag gcgttgatat
      421 taattcattc ggttttactg tgttgaagaa taaaaaaatc caaatgctag gtgaccatag
      481 catcgtcact ttttccacaa atttttataa gcctagtcac atcttgatga aacgactttt
      541 agatatactt ggagcagtag tcgggttaat tatttgtggt atagtttcta ttttgttaat
      601 tccaattatt cgtagagatg gtggaccagc catttttgct cagaaacgag ttggacagaa
      661 tggacgcata tttacattct acaagtttcg ttcgatgttt gttgatgccg aggtacgtaa
      721 gaaagaatta atggctcaaa accagatgca aggtgggatg ttcaaaatgg acaacgatcc
      781 tagaattact ccaattggac acttcatacg aaaaacaagt ttagatgagt taccacaatt
      841 ttataatgtt ctaattggag atatgagtct agtcggtacc cgtccaccta cagttgatga
      901 atttgaaaaa tatactccta gtcaaaagag aagattgagt tttaaaccag ggattacagg
      961 tctttggcaa gtgagcggaa gaagtgatat cacagatttt aatgaagtcg ttaggctgga
     1021 cctaacatac attgataatt ggaccatctg gtcagacatt aagattttat tgaagacagt
     1081 gaaagttgta ttgttgagag agggaagtaa gtaagactcc tttaaaacaa agaatagtaa
     1141 gtagtagggg atatgagaac agtttatatt attggttcaa aaggaatacc agcaaagtat
     1201 ggtggtttcg agactttcgt agaaaaatta actgagtatc agaaagataa atcaattaat
     1261 tattttgttg catgtacaag agaaaattca gcaaaatcag gtattacagg agaagttttt
     1321 gaacataatg gagcaacatg ttttaatatt gatgtgccaa atattggttc agcaaaagcc
     1381 attctttatg atattatggc tctcaagaaa tctattgaaa ttgccaaaga tagaaatgat
     1441 acctctccaa ttttctacat tcttgcttgt cggattggtc ctttcattta tctttttaag
     1501 aagcagattg aatcaattgg aggtcaactt ttcgtaaacc cagacggtca tgaatggcta
     1561 cgtgaaaagt ggagttatcc cgtccgacag tattggaaat tttctgagag tttgatgtta
     1621 aaatacgctg atttactaat ttgtgatagc aaaaacattg aaaaatatat tcatgaagat
     1681 tatcgaaaat atgctcctga aacatcttat attgcttatg gaacagactt agataaatca
     1741 cgcctttctc cgacagatag tgtagtacgt gagtggtata aggagaagga aatttcagaa
     1801 aatgattact atttggttgt tggacgattt gtgcctgaaa ataactatga agtaatgatt
     1861 cgagagttta tgaaatcata ttcaagaaaa gattttgttt tgataacgaa tgtagagcat
     1921 aattcctttt atgagaaatt gaaaaaagaa acagggttcg ataaagataa gcgtataaag
     1981 tttgttggaa cagtctataa tcaggagctg ttaaaatata ttcgtgaaaa tgcatttgct
     2041 tattttcatg gtcacgaggt tggaggaacg aacccatctt tacttgaagc actttcttct
     2101 actaaactaa atcttcttct agatgtgggc tttaatagag aagtagggga agaaggagcg
     2161 aaatactgga ataaagataa tcttcacaga gttattgaca gttgtgagca attatcacaa
     2221 gaacaaatta atgatatgga tagtttatca acaaaacaag tcaaagaaag attttcttgg
     2281 gattttattg ttgatgagta tgagaagttg tttaaaggat aagttatgaa aaagattcta
     2341 tatctccatg ctggagcaga attatatggg gcagataagg ttctcttgga acttataaaa
     2401 ggcttagata agaatgaatt tgaagcgcat gttatcctac ctaatgatgg agtcctagtg
     2461 ccagcattaa gagaagttgg tgcgcaagtt gaagttatta actatccaat tctacgtagg
     2521 aaatatttta atccaaaagg gatttttgac tacttcatat catatcatca ctattctaaa
     2581 cagattgctc aatatgccat agaaaataag gttgacataa ttcacaataa tactaccgct
     2641 gtcttagaag gcatttatct gaagcgaaaa ctcaaattac ctttgttgtg gcatgttcat
     2701 gagattattg tcaaacctaa attcatctct gattcgatca attttttaat ggggcgtttt
     2761 gctgataaga ttgtgacagt ttcacaggct gtggcaaacc atataaaaca atcacctcat
     2821 atcaaagatg accaaatcag tgtaatctac aatggggtag ataataaagt gtattatcag
     2881 tccgatgctc ggtctgttcg agaaagattt gacattgacg aagaggctct tgtcattggt
     2941 atggtcggtc gagtcaatgc gtggaaagga caaggagatt ttttagaagc agttgctcct
     3001 atactcgaac agaatccaaa agctatcgcc tttatagcag gaagtgcttt tgaaggagaa
     3061 gagtggcgag tagtagaatt agaaaagaag atttctcaat taaaggtctc ttctcaagtc
     3121 agacgaattg attattatgc aaataccact gaattatata atatgtttga tatttttgta
     3181 cttccaagta ctaatccaga ccctctacca acggttgtac tagaagcaat ggcatgcggt
     3241 aaacctgttg tcggttaccg acatggtggt gtttgtgaga tggtgaaaga aggtgttaac
     3301 ggtttcttag tcactccgaa ctcaccgtta aatttatcaa aagtaattct tcagttatcg
     3361 gaaaatataa atctcagaaa aaaaattggt aataattcta tagaacgtca aaaagaacat
     3421 ttttctttaa aaagctatgt aaaaaatttt tcgaaagtct acacctccct caaagtatac
     3481 tgattggctg aagtgaatgc tttagtatag cgatttatcg tattatcatt cgataaaaca
     3541 aatgttcaga aacagttata agttatttct aaagggcacc tctataaact cccaaaattg
     3601 cgaatttgga gttacgaaag ccttgttaa
//
LOCUS       DAZQCJ010000138         3553 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000138 DAZQCJ010000000
VERSION     DAZQCJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.140"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            78..782
                     /gene="yycF"
                     /locus_tag="ACHU66_002005"
     CDS             78..782
                     /gene="yycF"
                     /locus_tag="ACHU66_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFR3390259.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTREGYEVVTAFDGREALEVFEAE
                     FPDIVILDLMLPELDGLEVARTIRKTSNVPILMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELQARVKALLRRSELAETQTNIESTGTPELVIGDLVILPDAFVAKKHGKELELT
                     HREFELLHHLAKHLGQVMTREHLLETVWGYDYFGDVRTVDVTIRRLREKIEDTPSRPE
                     YILTRRGVGYFIKGND"
     gene            775..2124
                     /gene="vicK"
                     /locus_tag="ACHU66_002006"
     CDS             775..2124
                     /gene="vicK"
                     /locus_tag="ACHU66_002006"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HFR3390260.1"
                     /translation="MINQLRYLMTTAEFWFVVILIGFLIALTVLLIENYRDNKQIKQL
                     NQKVNALIEGNYADVLDMRGSPEITDMANSLNDLSEVIRLTHDNLEQEKTRLTSILSY
                     MSDGVIATDRIGRIIMINDMAQKQLGLSSQKQEQYHLLEVLDLSDRYTLRDLLAQTPE
                     IVIDHTNENEEFLTLRANFATIRSESGLISGLVVVLHDMTEQAKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALTESVAPSFVKVSLDETNRMMRMITDLLSLSRIDNQVG
                     QIDVELINFTAFVTFILNRFDQMKNTDSDKVYTIVRDYQISPIWVEIDTDKMTQVLDN
                     ILNNAIKYSPDGGTITFSMKTTDSQLIVSVSDEGLGIPKTDLPRIFDRFYRVDKARSR
                     AQGGTGLGLAIAKEIVKQHKGFIWAKSEYGHGSTFTIVLPYSKDIALDEWDDSDEEE"
     gene            2131..2934
                     /locus_tag="ACHU66_002007"
     CDS             2131..2934
                     /locus_tag="ACHU66_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFR3390261.1"
                     /translation="MIGKGFNYSILASGSSGNCFYLETDKKKILVDAGLSGKKVTSLL
                     AEIDRKPEDIDAILVTHEHSDHIHGIGVLARKYGMDIYANELTWQAMESKLGKIDVAQ
                     KHIFELGAMKTFGDLDIESFGVSHDAACPQFYRFMKDDKSFVMLTDTGYVSDRMVGIV
                     ENADAYLIESNHDIEILRSGSYSWNLKQRILSDKGHLCNEDGADAMIRSLGNRTKKIY
                     LGHLSKENNIKELAHMTMVNQLAQADLGVGVDFQVYDTSPDTATPLTKI"
BASE COUNT         1036 a          601 c          853 g         1063 t
ORIGIN      
        1 tgtcagatgg ttttgaaata tgaatttttt aggtaagtct gatataatag aagaaataaa
       61 gcaaaggtaa gatactaatg aaaaaaatat taattgtaga tgatgaaaaa ccaatctcag
      121 atattattaa gtttaatatg acgcgtgagg gatatgaagt tgtgacagct ttcgatggac
      181 gtgaagcctt ggaagtgttt gaggctgagt ttcctgacat tgtcattttg gacttgatgt
      241 tgccagaatt ggacggacta gaggttgctc gaaccattcg taagaccagc aatgttccaa
      301 tcttgatgtt atctgctaaa gatagcgaat ttgataaggt tatcgggctt gaaatcgggg
      361 cggatgatta tgtgaccaaa cccttctcta atcgcgaatt gcaggcgcgt gttaaggctc
      421 ttcttcgtcg tagtgaattg gcagagacgc agacaaatat tgagtcaaca ggaactccag
      481 agttggtgat tggcgatttg gtcattctgc ctgatgcgtt tgtcgctaag aagcatggca
      541 aagagctgga gctgacccat cgtgagtttg aattgctcca ccatctggcc aaacacttag
      601 gtcaggttat gactcgagaa catctattgg aaacagtttg gggttatgat tactttggtg
      661 atgtccgcac ggtggatgta acgattcgtc gtctgcgtga gaaaattgaa gatacaccaa
      721 gcagaccaga atacattctt actcgtcgcg gagtgggata ttttataaaa ggaaatgatt
      781 aatcaattac gttatttaat gaccacggca gaattttggt ttgttgtcat tttgattggc
      841 tttctaattg ctctgacggt ccttttgatt gaaaactatc gggataataa gcaaattaaa
      901 caactcaatc aaaaagtcaa tgccttaatt gaggggaatt atgcagatgt gttagacatg
      961 aggggaagtc ctgaaatcac agatatggcg aattctctta atgatctgtc cgaagtcatc
     1021 cgcttaactc atgataattt agaacaagaa aagacgcgtc tgacttccat cctgtcctat
     1081 atgagtgacg gtgttattgc tacagaccgt atcggacgga tcatcatgat taacgatatg
     1141 gcccagaaac agttggggct ttctagccaa aagcaggagc agtatcacct gctcgaggtt
     1201 ttagatttat cagaccgtta tacattgaga gatttgttgg ctcagacacc tgagattgtg
     1261 attgatcaca ccaatgaaaa cgaagaattt ttgactttac gtgctaattt tgccacgatt
     1321 cgtagtgaga gtggcctgat ttctggcttg gtagtcgtct tgcatgatat gaccgagcag
     1381 gccaaggaag aacgggaacg taggctgttt gtgtcaaatg tgagtcatga attgcgtacg
     1441 ccattgactt ccgttaaatc ctatctagag gctctggatg agggggcgct gacggagtct
     1501 gttgcaccga gttttgttaa ggtatcctta gatgagacca accgcatgat gcggatgatt
     1561 acagacctcc taagcttatc gcgtattgat aatcaagttg gtcagataga tgttgaactg
     1621 ataaacttta ctgcttttgt gacctttatt cttaaccgtt ttgaccaaat gaaaaatact
     1681 gattctgata aggtctatac gattgttcgt gactatcaaa tcagtcctat ttgggttgag
     1741 attgatacag ataagatgac tcaggtttta gataatatct taaataatgc cattaaatat
     1801 tcgccagatg gcgggacaat taccttcagc atgaagacca cagatagcca gttgattgta
     1861 tccgtatcag acgaaggtct ggggattccg aaaacagatt tacctagaat ttttgaccga
     1921 ttttatcgcg tagacaaggc acgatctcgt gcccaaggcg gtacaggtct tggtttagct
     1981 attgcaaaag aaatcgtgaa acaacataag ggctttatct gggctaaaag cgaatatggt
     2041 catggctcaa cgtttacaat tgtcttgccg tacagcaagg acatcgcact ggatgagtgg
     2101 gatgattcag atgaggaaga ataggagaat atgatcggaa aaggttttaa ttatagtatt
     2161 ttagcttcgg ggtccagcgg gaattgtttt tacctagaaa cagataaaaa gaaaatttta
     2221 gtggatgctg gcttatcagg gaaaaaggtt accagtcttt tggcggaaat tgatcgaaag
     2281 cctgaggata ttgatgctat cctggtaacg catgaacata gcgatcacat ccatggtatc
     2341 ggtgttttag cacgtaagta tggcatggat atttatgcca atgaattgac ctggcaggct
     2401 atggagagca aattgggcaa gattgatgtg gctcaaaaac atatcttcga attgggtgct
     2461 atgaagactt ttggtgacct agatattgag tcctttggag ttagccatga tgctgcatgt
     2521 ccgcaatttt accgttttat gaaggatgac aaatcctttg ttatgttgac ggatacaggc
     2581 tatgtcagtg accgcatggt tggaattgta gaaaatgccg atgcttattt gattgaatcg
     2641 aaccatgata ttgaaatttt gcgctcaggt tcttattcat ggaatttgaa gcaacggatt
     2701 ctatccgata agggacatct ttgtaacgaa gatggagctg atgccatgat tcgctcgttg
     2761 ggaaatcgga ccaaaaagat ttacctaggg catttgtcaa aggaaaacaa tatcaaggaa
     2821 ttggctcaca tgaccatggt taatcaattg gctcaggctg atttaggagt tggagtggat
     2881 ttccaagtct atgacacatc gccagataca gcgactccct tgaccaagat ttgacaattg
     2941 atacaaacta agttatactg aaattactaa ttatacgagg tgaagttata tgaataatta
     3001 agctgcttat tagtcgttct taaaacttgt agtgaggttg tattaaccga tgatacaagg
     3061 gataagactg ctcataaggg cttactattc gtgtgacttt ttccataggg aaaggtcatg
     3121 ttttgcatgg cttttttctt gttcattgag cagaataggg gccctttttg tgaagaaaag
     3181 aggtctatat ggaagtaata aaatgtattc gattagagaa ggaatttgcg ggatgtagag
     3241 gctgggcaaa aagcttaacc tcgcttctca gggttcgtgt caacatctca gcgcagtggt
     3301 tgattggcag atttgttcgt gttttacact ccaaatctga ccttacgatc gttgcgaaca
     3361 aagttcgctt tatctccaac ctcaaactgt ctcccagaca gtttgagctg tgcgggggtg
     3421 ggagtgaaac agtctgggga tagactgttt cagctcaaca actggaaata aggacttgtt
     3481 gacgaactct tttttctacc atttgtcaag tccatcaagg tatttgacga aaaatatttt
     3541 gtgtctcgta atc
//
LOCUS       DAZQCJ010000139         3480 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000139 DAZQCJ010000000
VERSION     DAZQCJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.141"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(25..369)
                     /locus_tag="ACHU66_002008"
     CDS             complement(25..369)
                     /locus_tag="ACHU66_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SSU0592/SSU0593 family protein"
                     /protein_id="HFR3390262.1"
                     /translation="MKHIVTKRNYKVGACAMALALLMTTAGGAIVNAEQRLNSPVGFE
                     QRDEVLLELKESTQPFGQGVQHLEPINSPATQMAPSVQKSSWFSSFWGGVKTFWNALC
                     CSVGLTGLKVSK"
     gene            complement(836..1540)
                     /locus_tag="ACHU66_002009"
     CDS             complement(836..1540)
                     /locus_tag="ACHU66_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR3390263.1"
                     /translation="MKIHVVNNQVEGATVALDILREKLNGGAKVLGLATGSSPLEFYR
                     LIRESDLDFSNVTSVNLDEYVGLGEESDQSYIHFMKENLFNDKPFKKSYLPNGLATDV
                     VAETERYNKILAEHPVDFQILGIGRNGHIGFNEPGAPFDGQTHLVELAPSTIEANARF
                     FDNPEDVPKQAISMGIANIMAAKTIVLMAYGQEKADAIKATVEGAVTEDVPASVLQNH
                     DNVILILDQAAASKLV"
     gene            1888..2916
                     /gene="queA"
                     /locus_tag="ACHU66_002010"
     CDS             1888..2916
                     /gene="queA"
                     /locus_tag="ACHU66_002010"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR3390264.1"
                     /translation="MNTADFDFDLPEELIAQVPLEKRDSSKLLILDREKQSMVDSHFD
                     HIIDQLNPGDALVMNNTRVLPARLYGYKPETMGHVELLLLKNTQGNQWEVLAKPAKRL
                     KVGTTVAFGDGRLTATIVEELEHGGRIVEFSYEGIFLEVLESLGEMPLPPYIHEKLED
                     RERYQTVYAKENGSAAAPTAGLHFTQELLEKIEAKGVKLVYLTLHVGLGTFRPVSVDN
                     VDEHEMHSEFYNLSAEAAQTLNQVKDSSGRIVAVGTTSIRTLETIGNKFDGRLEADSG
                     WTNIFIKPGYTFRIVDAFSTNFHLPKSTLVMLVSAFAGREFTLDAYKHAVEERYRFFS
                     FGDAMFIQ"
     gene            2965..3438
                     /locus_tag="ACHU66_002011"
     CDS             2965..3438
                     /locus_tag="ACHU66_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFR3390265.1"
                     /translation="MNKIESRHQLILSLIMEKKIHTQQELQELLEINGVSVTQSTLSR
                     DIKMLNLVKVNEDDSSHYVINPIAPTRWEKRLRLYMEDALVMLKPIQHQVVLKTLPGL
                     ANSFGSILDAMEIPQIVATVCGDDVCLIICEDVEGAQACFEHLKQFTPPFFFSKL"
BASE COUNT         1037 a          749 c          618 g         1076 t
ORIGIN      
        1 atattacaag attataactc tatgttattt actaactttg agcccagtta gtcccacaga
       61 acaacataat gcattccaga atgtttttac accaccccaa aatgaactaa accatgatga
      121 tttttgtaca gaaggggcca tttgagtcgc aggtgagttg ataggctcta aatgttgaac
      181 accttgacca aaaggttggg ttgattcttt cagctctaga agtacttcgt cacgctgctc
      241 aaaaccaact ggtgaattaa gtcgttgctc tgcatttaca attgcaccac ctgcagtagt
      301 catcaacaat gcaagtgcca tagcgcatgc gccaacttta taattacgtt ttgttacaat
      361 atgtttcatg atgaaacatc tcctttatat agatatttgc ttcctcctat cggaaacgta
      421 gtaacagtat agcacaaaaa aataactcaa aaaccaactt tagaaaaaga ttatataaaa
      481 accagaaaac tacctgaaaa aattgaaaac gttatacaaa acgtagaaaa aatgacacta
      541 tttcgctcat ttcacttgtt cctatcaatt ttcaacatga taaaggatag caatatctct
      601 tgttgaaatt ttctctcaca tccaacttaa tgttatatat aatccattca aatatataac
      661 aactgatatg gattatcaga atagcaaaag gcatgccctt aaaaatataa gttacatgcc
      721 ttttcacaat ttttgtcaaa ttcataatgg aaaaagaacc tagattcatt cagaaattat
      781 acttattgta ctgttacaac caactttatg tctaagaata atcgtttaca acatactaca
      841 ctaatttact cgctgctgct tggtcgagga tgaggatgac attgtcatgg ttttggagga
      901 cgcttgctgg gacatcttct gtcactgccc cttccacagt cgccttaatg gcgtcagctt
      961 tttcctgacc ataggccatg agaacaatgg tcttggctgc cataatgttg gcgatgccca
     1021 tggagatagc ttgttttggt acatcttctg gattgtcaaa gaagcgggcg ttggcttcaa
     1081 tggttgatgg cgccaattca accagatgag tctgaccatc aaatggagca cctggttcgt
     1141 tgaaaccgat atggccgttg cgaccgatac ccaaaatttg gaaatcaacc ggatgttcag
     1201 ccaaaatttt attgtaacgt tctgtttcag caactacatc tgtcgccaaa ccattcggta
     1261 aataagattt cttaaacggt ttgtcattga aaagattttc cttcataaaa tgaatatagg
     1321 attggtcgct ctcctcacct agaccaacat actcatccaa gtttacagat gtcacattcg
     1381 aaaagtctaa atctgactca cgaatcaatc tgtaaaattc taacggactg gagcctgttg
     1441 ccaaacctaa taccttggca cctccgttca atttttctct caaaatgtct aatgctacag
     1501 ttgctccttc aacttgattg tttactacat gaattttcat aacacacctc tttggtctat
     1561 accatttatt gtttttcttt attatatggt atagaccaat ttttgtcaag aatttatacc
     1621 tatagagtat tacatcagca cgaataattt taattctctt cccatccatc aagacttaag
     1681 tgtctaacta tgatataata catttatgac agatgtgctt ttttacttat tttttattgg
     1741 tattctgttc tgccttactg gctattttat ttctaaatca aaggtcctaa agtttatttt
     1801 ctacctgatt ggtagtctat tggtggcttt gccatttgcc ctattgattt attttaccta
     1861 tattttattt tagtctaagg agtgaatatg aatactgcag attttgattt tgatttaccc
     1921 gaagagctta tcgctcaagt tccccttgaa aagcgcgaca gctctaaact attgatcctc
     1981 gaccgcgaaa aacagagcat ggttgacagt cactttgacc atatcatcga ccagttgaat
     2041 cccggtgatg ccctggtcat gaacaataca cgtgttctgc ctgcccgtct ctatggctac
     2101 aagcctgaaa ctatgggaca tgttgagctt ttgcttttga aaaataccca aggtaatcag
     2161 tgggaagttc ttgccaaacc agcaaaacgt ctgaaggtcg gtaccacagt cgcttttggt
     2221 gacggacgat taactgctac tatcgtagaa gaattagaac acggtggtag aattgttgaa
     2281 ttctcctatg aaggtatctt ccttgaagtc ttggaaagtc taggcgaaat gccacttcca
     2341 ccttacattc atgagaaatt agaagaccgc gaacgctatc aaacagttta tgccaaggaa
     2401 aacggttctg ccgcagctcc aactgctgga ctacatttca cccaagagtt actcgaaaaa
     2461 atcgaagcta aaggagtgaa actagtctat ctgactcttc acgttgggct tgggaccttc
     2521 cgaccagtct ctgttgataa cgttgatgag catgaaatgc actcggaatt ctataaccta
     2581 tcagcagaag ctgctcaaac cctcaatcag gtcaaagata gcagtggtcg tatcgtcgca
     2641 gtcggcacga catccatccg caccctcgaa accatcggta ataaatttga tggtcgtttg
     2701 gaagctgatt ctggttggac aaatatcttc atcaaacctg gctacacctt ccgaatcgtt
     2761 gatgccttct caaccaattt ccacctgcca aaatctactt tggttatgct agtttctgcc
     2821 tttgcaggta gagaatttac cttagatgcc tataaacatg ctgttgaaga acgctaccgc
     2881 ttctttagtt ttggggatgc aatgttcatt caataggctg ttatcaccaa attattctga
     2941 attctgattt tattggaggg ccctatgaac aagattgaaa gccgccatca gctgatcctg
     3001 tctctaatca tggagaagaa aatacatacc caacaggaat tgcaagaatt actagagatc
     3061 aacggagtct ccgtcaccca atctaccctc tcccgtgaca tcaagatgct caatcttgtc
     3121 aaagtcaacg aagatgattc ttctcactat gtcatcaacc ccattgctcc tactcgctgg
     3181 gaaaaacgac tgcgactcta tatggaagat gctttggtca tgctaaaacc tatccaacat
     3241 caagtggtgc tcaagaccct acctggattg gccaactcat tcggttctat tttggatgcc
     3301 atggaaattc ctcaaattgt agcgaccgtc tgcggtgacg atgtctgtct catcatttgt
     3361 gaagatgtcg agggggcaca agcttgcttc gagcacttga aacagtttac tccaccattt
     3421 ttctttagta aattataaaa atttatgttc acaaacaaat acttaatttt aaagataatt
//
LOCUS       DAZQCJ010000140         3444 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000140 DAZQCJ010000000
VERSION     DAZQCJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.142"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..926
                     /locus_tag="ACHU66_002012"
     CDS             <1..926
                     /locus_tag="ACHU66_002012"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFR3390266.1"
                     /translation="AKPTGTITIENRNDAQGTFDVRVTNVSSPKDIASVLLPTWSQSD
                     DIRWYEATRQSDGSYKLTVNKKDHKYRTGTYTVHLYYKDSNGGLTGAGGTTTHLSNPS
                     AQRSYTVYIDPGHGGRDSGASYGGVHEKNLALSVSNKLRENLLQYGINVLMTRTGDYD
                     VDFKTERSRMTNASNADLFISIHFNATGAGVSNATGIETYWYQYNPEYQPKINKEMHN
                     NPTRLAESEILANKVQESLIKETGAVNRGVRRETFAVLRETAIPAILVELGFMDNPSE
                     LQVIKQDSYHTRLAKALAQGVMNWYGAVEGK"
     gene            1004..1432
                     /locus_tag="ACHU66_002013"
     CDS             1004..1432
                     /locus_tag="ACHU66_002013"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFR3390267.1"
                     /translation="MEQSSKANSILSSVMLSQAILESAWGTSYLATHGNNLFGIKADA
                     AWTGATIEIITNEYRDGEKKQEKHLFRKYNSWNESVADYAKFFTSTPWRIKNYQSFRE
                     ATDYQQAILALRQSGYATDPKYGEKLRSIIENYKLYLLDN"
     gene            complement(1492..>3444)
                     /locus_tag="ACHU66_002014"
     CDS             complement(1492..>3444)
                     /locus_tag="ACHU66_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFR3390268.1"
                     /translation="FTFPRLYRNELVLFNSNFNKMETQQLLVALTFLIFFGLLGYKQL
                     RGHISKAFKTISLSVIYLLVPIFSLFIIENSYSQFSTLSSNSLWNNLIIIGVLYLILF
                     FTTNLRFASLLILSASVFIGISNQLLIDSRGAPLLFYNLFQIIDGLNVASSVAININN
                     RMLQSMVFSYILLTFFFFIPKLYLPKLLPSRTFYSSYDFKWPKRFSRILLGYITLITI
                     VPMINKTVVSNANISLNYWRMYVTYGQFGLPLSLASFYEDSKITKPEGYSVPKLNEVL
                     EKYPPETEKQTIRPNIIFIQNESQSDFSNLQGLNMEPDPLSNQHALTDNTIHGTLNVS
                     VFGGGTANTEYEVLTSNPISLLSSNLFPYQQIITQERPSFATYLKNKNYDTVALHPQS
                     GNNYNRNAVYPLLGFNKSYFLDSEPAISSLAPLTIDRGWPSDQFLFNGIKELYTQKGD
                     QPLFSFVVTMQGHGGYPSTEEIYPREVSINGSTSEYLAETEFLTSMKRTDEAFADLIT
                     FFSTYKEPTVIVMYGDHQPSLTQEFYAQFMDENNPAAKYSTPLVIWSNFDIRERESTT
                     ISPNYLVPYLMDILSESDYALPRSPYQQFLSDMQIEAPIITSWGNIDNNGQQIEDLST
                     LPLYQTYLQLEYNSAIDKQPLTDLYE"
BASE COUNT         1119 a          550 c          733 g         1042 t
ORIGIN      
        1 aagcgaagcc aacaggtacc atcacaattg aaaatcgtaa tgatgcccaa ggtacctttg
       61 atgttcgtgt tactaatgtt tcatcaccga aagatatagc aagtgttctc ttacctacct
      121 ggtcccagtc agacgatata cgttggtatg aagctactcg tcagtcagat ggcagttata
      181 agttaactgt taataagaag gaccataagt accgtacagg tacctacacg gttcacttat
      241 attataagga ttcaaatggt ggtttgacag gtgcaggtgg aacaacaact catttatcaa
      301 atccgtcggc acagcgatcc tacacagtct atattgaccc agggcacgga gggcgagact
      361 cgggtgcttc ttacggtggc gtacatgaaa aaaatcttgc attgtctgtt tcaaataagt
      421 tgcgtgaaaa tctcttgcaa tatggaatca atgttttgat gacccgtaca ggtgattatg
      481 atgtagactt caaaacagag aggtcacgaa tgacgaatgc aagcaacgca gacttattta
      541 ttagtatcca ttttaatgcg acgggtgcag gagtttcgaa tgcgactggt atcgaaacat
      601 actggtatca atataatcca gaataccaac ctaaaattaa taaagaaatg cataataacc
      661 ctactcgttt agctgaaagt gagattttag ccaacaaggt ccaggagagt ttaattaaag
      721 aaacaggtgc agttaaccgt ggtgtaagaa gagaaacttt tgcagtcctt cgcgagacag
      781 caattcctgc cattctagtg gagctaggat ttatggataa tccttcggag ttgcaagtta
      841 tcaaacaaga ttcttaccac actcgccttg cgaaagcatt ggcacaaggt gtaatgaatt
      901 ggtatggtgc tgttgaggga aagtagaaat tgcaggtaca ggtattgaaa caggaaaact
      961 ttctaatgat caaaaaagtt tcatagccaa tctagtacct gcaattgaac aaagttcaaa
     1021 agctaattct atcctttcga gcgtcatgtt atctcaagcc atcctcgaaa gtgcatgggg
     1081 aacgtcatac ttggcgactc atgggaataa tctttttggc atcaaagcgg atgctgcgtg
     1141 gacaggtgct acaattgaaa tcattaccaa tgaataccgt gatggtgaga agaagcaaga
     1201 aaagcacttg tttagaaaat ataattcatg gaatgaaagt gtagcagact atgctaagtt
     1261 ctttacctct actccgtgga gaataaaaaa ttaccagagt ttcagagagg caactgatta
     1321 tcaacaagcg attcttgcgc taagacaatc aggatatgct actgatccga aatatggtga
     1381 aaaattacga tcaattatcg agaattacaa gctttatctt ttagataatt aagttttttg
     1441 ggtgattgat gataaaatag ccttgctcaa aaccgagcaa ggctattttt atcactcata
     1501 taaatctgtt aaaggttgtt tgtcaatggc actattgtat tctagttgta agtaggtttg
     1561 gtaaagtggt agggttgata ggtcttcaat ttgttggcca ttattatcga tatttcccca
     1621 gctagtaatg ataggtgctt cgatttgcat atcactcaaa aattgttggt aaggtgatcg
     1681 aggaagggca tagtcggatt cggataaaat atccatcaaa tatggaacga gatagtttgg
     1741 gctgattgtt gtagattcgc gctccctaat atcaaaattc gaccaaatca ctaaaggggt
     1801 agaatattta gcggcaggat tattttcatc cataaattgt gcgtaaaatt cctgagtcaa
     1861 actaggttgg tgatcgccgt acatgacgat gactgttggc tctttgtaag tgctaaagaa
     1921 agtaattaaa tctgcaaatg cttcatctgt tcttttcatg ctggtcaaaa attctgtttc
     1981 agctaagtat tcagatgttg aaccattaat gctgacttcg cgaggataaa tttcttccgt
     2041 acttggatag cctccatgtc cttgcatggt aacaacaaag ctaaatagag gctggtctcc
     2101 tttttgggta taaagttctt ttattccgtt gaacaagaat tgatcagatg gccagccacg
     2161 atcgattgtt agtggtgcta agctactaat agcaggttcc gaatctaaaa aataagattt
     2221 attaaacccc agtagaggat acactgcatt gcggttgtaa ttatttccgg attgaggatg
     2281 tagagctaca gtgtcgtaat ttttattttt tagataagtt gcgaaacttg gacgttcttg
     2341 tgtgataatc tgctgatatg ggaataaatt agaagataat aaactgattg ggttacttgt
     2401 cagcacttca tactcggtat ttgcagtacc accgccaaag actgagacat tgagggtgcc
     2461 atgaatagta ttgtctgtta atgcatgttg gtttgatagc ggatcaggct ccatatttaa
     2521 gccctgtaag tttgaaaaat cactctgtga ttcattctgt ataaagataa tattaggtct
     2581 gattgtttgt ttttctgttt caggagggta cttctcaaga acttcattaa gtttgggaac
     2641 tgaatatcct tcaggcttag ttattttact atcttcatag aaagaggcga gtgagagtgg
     2701 tagcccaaat tgaccatagg tgacgtacat tctccagtag tttaatgata tatttgcatt
     2761 agagacgaca gttttattaa tcattggaac gattgttatt aaagttatat agcccaataa
     2821 aattctgctg aaacgtttcg gccatttgaa atcataacta gaataaaacg ttctagaagg
     2881 tagaagtttt ggtaaatata atttagggat aaagaaaaag aaggtcaata agatataaga
     2941 aaataccatg ctttgtagca ttcgattgtt gatattaatt gctacgctag aagctacatt
     3001 taatccgtct atgatttgaa aaagattata aaataataga ggagcaccac ggctgtcaat
     3061 caaaagttgg ttacttatac caataaatac tgaagcggat agaattaata ggctggcaaa
     3121 tcgtaagttc gttgtaaaga acagtattag gtagaggact ccgataatga ttagattgtt
     3181 ccaaagactg tttgaggaaa gagtagagaa ttgtgagtag ctattttcaa taataaataa
     3241 tgaaaaaatg ggaactagaa gataaatcac tgatagacta atcgttttga aggctttaga
     3301 gatatgacct cggagttgtt tgtatcccaa aagaccaaaa aatatcagaa aagttagtgc
     3361 gactaatagt tgttgagttt ccattttatt aaagttcgaa ttaaatagca ctaattcatt
     3421 gcgatataaa cgtggaaatg taaa
//
LOCUS       DAZQCJ010000141         3354 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000141 DAZQCJ010000000
VERSION     DAZQCJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.143"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            163..2139
                     /locus_tag="ACHU66_002015"
     CDS             163..2139
                     /locus_tag="ACHU66_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HFR3390269.1"
                     /translation="MNFEEWIWALPSLSSFGEAIEKGLVMQTIFGKIVANQSAESVKT
                     YILNQQDAAYKELLLSCQNLLNDIFIFEDNWDMEPCSVGYHLAPLDWTADCHGDEEWT
                     FMLNRQEYLWKLVLAYLVEKDSRYMEQVKRFIFSWIEQVTNWHPKRTSSRTLDTAIRC
                     LSWLNILPFLIDWGGLSEAERSQLLVSIQKQLSYLHSHYRAKDTLSNWGIFQTGAMLL
                     AHAYIGDQVDLVELHHFAHQEIQEQIQTQILEDGTQFEQSFLYHVEVYKLLLGLAFSL
                     PDYRKQWTPILKKMADYVLQMTGPDGKSVALGDSDVHCTSDILQMTAIFFEDGTYLVD
                     NSKLDLGVLFLVGENGLRIFEHLTAQPNSSGGQHFSESGHSIVKEVDSYLCFKAGPVG
                     SAHSHSDQNSLCFYHKGQPIVIDSGRFSYRECEERYLLKSAWAHSTCILDDNAPEQIK
                     GSWEYDTYPQFISHSYKGVERCHWMQGIYQAQTSQGLSYWHKRQLLMVGGDLLLIVDS
                     VDCSGQHELISQLILDERVKIGEERLNDLRLYSQKDFEIQKTVRSPRYNQLVETNKLV
                     KKESFQDRLVTFTLLADEAIRVKQLPVYQTDYHLIESGLAFSCQGKGLNILLVVSDND
                     IYKGEKLLQVAGYKLRGKCLVVDRDKRQVVRLKN"
     gene            2235..3236
                     /locus_tag="ACHU66_002016"
     CDS             2235..3236
                     /locus_tag="ACHU66_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR3390270.1"
                     /translation="MSEKFTIKDIAELAQTSKTTVSFYLNGKYEKMSKETRERIERVI
                     RETDYKPSVVARSLNSKNTKLIGVLIGDITNTFSNQIVKGIEEEAHKSGYQVIIGNSN
                     YNQESEDKYIESMLMLGVDGFIIQPTSHFRKYSRIIEDKKKHMVFFDSQLYEHRTSWI
                     KTNNYDAVYDATQLCVENGYQKFILITADISRLSTRIERATGFMDALLDCKQSYQDLT
                     IADENTDIEEIKRFLQGVVNPQEKTLVFVPNCWALPLVFNAMKELKYDFPQVGLLGFD
                     NTEWTNFSSPTISTIVQPAFEEGCQATRMLINQIEGGNQVGRQQVLDCTVHWKDSTF"
BASE COUNT         1050 a          600 c          739 g          965 t
ORIGIN      
        1 tctatcccca gactgtttca ctcccacccc cgcatagttg attaaatcag attcggagta
       61 tgaaatacga attgctgaaa tttgttttgg aactcctatc tccaaccttt aacagtccac
      121 tgaactgtta aagccaatca atcactgcgc taagatgttg atatgaactt tgaggagtgg
      181 atctgggctt tgcccagcct ctcttctttt ggagaagcga tagagaaagg acttgttatg
      241 caaacaatat tcggcaaaat agtagccaat cagtcagcag agtcggtcaa aacctatatt
      301 ctcaatcaac aagatgcagc ttataaagag cttctccttt cttgtcaaaa tttattaaat
      361 gatattttta tctttgaaga caattgggat atggaacctt gttcagtcgg gtatcatctt
      421 gctcctcttg actggacagc cgactgccat ggggatgagg aatggacctt tatgctcaat
      481 cggcaggaat acttgtggaa attagttttg gcctatctgg ttgaaaaaga ttcgcgctac
      541 atggagcagg ttaaacggtt tatctttagt tggatagaac aagtgacaaa ttggcatcct
      601 aaaagaacga gcagtcgcac cttagatacg gctatccgct gtctaagctg gctcaatatt
      661 ctcccatttt taattgattg gggcggacta agtgaagctg agcgtagcca actgttagta
      721 tccattcaaa aacaattatc ttatcttcac agtcattacc gtgccaaaga tacgttgagc
      781 aattggggaa ttttccagac tggcgcaatg ctcttggccc atgcttatat cggtgatcag
      841 gtggatttag tagagcttca ccattttgct catcaggaaa tccaagagca aattcaaact
      901 cagatattgg aagatggaac tcagtttgag cagtcttttc tctaccatgt ggaagtttat
      961 aaactcttgc taggactagc tttcagcttg ccagattatc gaaaacagtg gacacctatt
     1021 ttgaaaaaga tggcagacta tgtcttacag atgacgggac cagatggcaa aagtgtggct
     1081 cttggcgata gcgatgttca ttgtacttct gacatcttac agatgactgc gattttcttt
     1141 gaagatggca cctacctagt agataattcc aagctagatt taggcgttct cttcttggtc
     1201 ggagaaaatg gcttgcgcat atttgaacac ttgaccgctc aacccaactc gtcaggggga
     1261 cagcattttt ctgaatcagg tcacagtatt gtcaaagaag tggacagcta tttatgtttc
     1321 aaggccggtc ccgtgggaag tgcccacagc cactctgacc aaaatagctt gtgcttttac
     1381 cataaaggac aaccaatcgt aattgacagt ggtcgtttca gctaccgtga atgcgaggag
     1441 cgctacttgc ttaaaagtgc ttgggctcac agcacttgta tccttgatga caatgctcca
     1501 gagcagataa aaggttcatg ggaatatgac acgtatcctc agtttattag tcacagttat
     1561 aagggggttg aacgctgtca ttggatgcag ggcatttatc aggctcagac cagccaaggc
     1621 ttgtcttact ggcataaacg acagctcctt atggttggag gcgatttatt gctgatagtg
     1681 gatagcgttg attgctcagg ccagcatgag ttgatcagtc aattgatttt agatgaaaga
     1741 gtcaagattg gagaagagcg gttaaatgat cttcggctat atagtcaaaa ggattttgaa
     1801 atccagaaaa ccgtgcgttc tcctcgatac aaccaactag tagagaccaa taaactggtc
     1861 aaaaaagaga gttttcaaga tagattagtt acatttactc tattagcaga tgaagcaatt
     1921 cgtgtcaagc aattgccagt ttaccaaaca gactatcatt tgatagaaag tggtttggct
     1981 tttagctgtc aaggaaaggg actgaatatt ctgttagtgg tttctgataa tgatatatat
     2041 aagggagaga aattgcttca agtggctggt tataagttga gaggtaagtg cttagtagtg
     2101 gatagggata agagacaagt tgtccgactc aaaaattaga agggacagaa aaaatacagt
     2161 catttctgtg caaaataact gataaagtac ggatagtatg atagaataaa gtaactaaag
     2221 gaggtatcgg taaggtgtca gagaagttta ccatcaaaga tattgcagaa ctggctcaga
     2281 cttcaaaaac aacagtttcc ttttacttaa atggtaaata tgaaaagatg tccaaagaga
     2341 cacgcgaaag aattgagaga gtcattcgcg aaacagacta taaacccagc gttgttgcgc
     2401 ggagcctaaa ttccaaaaat actaagttga ttggagtatt gattggtgat attacaaata
     2461 cattttccaa tcaaattgtc aaaggtatag aagaagaagc gcataagagt ggctatcaag
     2521 ttatcatcgg aaacagcaat tacaatcaag aaagcgaaga taagtatatt gagagcatgc
     2581 tgatgttggg tgtagatggt tttattattc agccgacatc gcattttaga aaatattcgc
     2641 gcattattga ggataagaaa aagcatatgg ttttctttga tagtcagctt tacgaacacc
     2701 gtaccagttg gattaagaca aataattatg atgctgtcta tgatgcaact caactatgtg
     2761 tagaaaatgg ttatcaaaaa tttattctta ttacagctga cattagtcgc ttaagtacac
     2821 ggattgaacg agctacaggg tttatggatg ctctgttaga ttgtaaacaa agttatcaag
     2881 atttaacaat tgcagatgaa aatacggaca tagaggaaat taaacgtttt cttcaagggg
     2941 tggtaaatcc tcaggaaaag accctagtat tcgttccgaa ttgctgggcg ttacccttag
     3001 tttttaatgc tatgaaagaa cttaaatatg actttcctca ggttggtctt ttggggtttg
     3061 acaatactga atggacaaat ttttcatccc ctactatctc taccattgtt caacccgcct
     3121 ttgaagaagg gtgtcaggca acaaggatgc tgatcaatca gattgagggc ggaaaccaag
     3181 tgggacgaca acaggtttta gattgcacag ttcattggaa agattctaca ttctaagtta
     3241 agtcaaattt tacttcttag gaagaggagc agaacgaagt agaatattct gattgagagc
     3301 tcactgaaac cctccttgaa tcgcctaata tatacaaaaa ataggaattt tccc
//
LOCUS       DAZQCJ010000142         3264 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000142 DAZQCJ010000000
VERSION     DAZQCJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.144"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            54..1715
                     /locus_tag="ACHU66_002017"
     CDS             54..1715
                     /locus_tag="ACHU66_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390271.1"
                     /translation="MKRKLFVFAWLALVLEMLIYYWYQLPALNILSVDFWIFFLQSVG
                     LVWLILSMFSSKGAFQKVTKISGRQRQVDTYQIKTSQLPAYLKWLGRIWVLVVLGLLG
                     LGLINSRVFRAKDYAAVISVKDADFKADFPETDISKLALLDRASAEKIGDTYLGTIDK
                     VSQFGISDDYRQITIGQQPFRVSPLEYKNFWKWLSNHQDGIGYYVKVNQTTGKAELAK
                     LSKAMHYSDSEFLLNDTLRHLRLQYPTTIFGKPSFEVDDQGNPYYIATIYEPKFGLSS
                     NDPIGAIVLDAVTGESKKYSLEDIPEWVDRVYSANNVISRVDDHYTYQNGFWNTIFSQ
                     TGVKNTTDSYNYISIGSDIYLYTGITSATADSSNLGFILVNMRTREITNYKLASATET
                     AAQESAEGEVQEKGYKATAPSLVKLADTAYYLVSLKDDAGLVKSYALVDAEDYQQVTV
                     NNDIALLISQVTGTDASSLSGLTVTENGEVGEQTEVISGKVEALASQIIGGATIYYIE
                     SEGKIYKVKATEDSTDRLPFIKVGDHFSGQLDKKNYLKNFKVNPE"
     gene            1875..3263
                     /locus_tag="ACHU66_002018"
     CDS             1875..3263
                     /locus_tag="ACHU66_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR3390272.1"
                     /translation="MSLFRKKQAVGRHSQMRRHLGLVDLIFLGIGSMVGTGIFTVTGL
                     AAAQYAGPALIISIVIAAISVGLTALFYAEFASRIPTNGGAYGYLYSVFGEFPAWIAG
                     WLTIMEFLTAVSSVASGWGAYLKGLLANFGIAMPTALNGTFNPAAGTYVDLLPVLVLI
                     FVVGVVLLNSKAALRFNSALVVLKFSALALFILVGLFFIKPENWSNFSPFGFGAIYGG
                     QAGIMAGASLMFFAFLGFESISLAIDEVKKPEKNVPKGIVLSLSIVTILYIVVTLVLT
                     GMVHYTKLNVADAVAFALREVGLDWAASYISIVAILTLITVCISMTYALSRMVYSISR
                     DGLLPKSLSRLTQTSKVPKNATILVGIFAAICAGIFPLASIASFLNICTLAYLIMLAL
                     GIIRLRQVEGLPKDGQFKTPLVPLLPILSIIICLSFMFQYSLDTWLAFGVSVVIGILI
                     YFCYGYRHSEAK"
BASE COUNT          881 a          602 c          729 g         1052 t
ORIGIN      
        1 acattaaaat tgtgatacaa tagaactaat aaatctgtgt caaggaggtt attatgaaaa
       61 gaaaattatt tgtttttgcc tggttagccc tagttttgga aatgctaatc tactactggt
      121 accagttgcc tgctttaaat attttgagtg tggacttctg gatatttttc cttcaatctg
      181 tcggattggt ctggctcata ctttccatgt tcagctccaa aggtgctttt caaaaggtga
      241 ccaagatttc cggtcgccag agacaagtag atacctatca gattaaaacc tcgcaactgc
      301 cagcttactt aaaatggttg ggacgaattt gggtattggt tgtcctgggt ttgttggggc
      361 tggggcttat caactcacgt gtatttcgtg ctaaggatta cgctgctgtc ataagcgtta
      421 aggatgcgga ttttaaagca gattttcctg aaacagacat atccaaattg gccctcctag
      481 accgcgcctc ggccgaaaaa atcggtgata cttatctagg taccattgac aaggtctccc
      541 agtttggcat ttcggatgat tatcgccaga ttactatcgg tcagcaaccg ttccgtgtat
      601 cgcctttgga atataagaat ttttggaaat ggctgagcaa ccatcaagat gggattggtt
      661 actacgtgaa ggttaatcag acaacaggga aggcagaact tgccaagttg agcaaagcca
      721 tgcactattc agattccgaa tttttgctga atgatacctt acgtcatctt cgcttgcaat
      781 acccgacaac tatttttggg aaaccatcct tcgaagtgga tgaccaagga aatccatatt
      841 atattgcaac gatttacgag ccaaaatttg gactttcatc aaatgatccg attggtgcta
      901 ttgtattgga tgcagtaaca ggggaaagta agaagtatag tctggaagat attccagagt
      961 gggtagaccg tgtttattcg gcaaacaatg ttatcagccg tgtagatgac cactatactt
     1021 atcaaaatgg tttctggaat accattttca gtcaaactgg tgtgaaaaat acaacagaca
     1081 gctataatta tatttctatc ggctcagata tttatctcta cacaggtatc acctcagcaa
     1141 ctgctgattc atccaacctt ggatttattc ttgtcaatat gagaactcgt gaaattacca
     1201 actacaaatt ggcttcggcg acggaaacag cagcgcaaga gtccgcagaa ggtgaagtgc
     1261 aggaaaaagg ctataaagcc actgctccaa gcctggttaa gttggcagat acagcctact
     1321 atttagtatc cttgaaagat gatgccggct tggttaaatc atacgctctt gttgatgccg
     1381 aagattatca acaggttact gttaacaacg atattgcgct cttaatttct caagtcacag
     1441 gaacggatgc ttctagcctt tcaggattga cggtgactga aaatggagaa gttggtgaac
     1501 agactgaggt tatcagtgga aaagtagaag ctttagcgag tcaaataatt ggcggtgcga
     1561 caatctacta tattgagtct gagggcaaga tttacaaagt caaagcaacg gaagatagta
     1621 cagatcgatt accatttatc aaagtaggag atcactttag cggtcagtta gataagaaaa
     1681 attatcttaa aaactttaag gttaatccag aataatcata agaaagagtg aggtagaaaa
     1741 gccttgctct tttgcttgtc gtcacccatg cgaaatattt caacttatgg tataatgggg
     1801 cgttgtggtt ctttgacagg aatccgtaga ttattctgtc gagtatccat attcttattc
     1861 tgaaggagta ttttgtgagt ttatttagaa aaaaacaagc agtcggtcgc catagtcaaa
     1921 tgcgtcggca tttaggctta gttgatttga tatttttagg tatcggttcg atggttggaa
     1981 caggtatctt tacggtaact ggtttggcag cagcccagta tgcagggcca gctctgatta
     2041 tctcaattgt tattgcagca atttcagtcg gactaacagc cttgttttac gcagaatttg
     2101 cctcgcgtat accgactaac ggtggtgcct atggttatct ctactcggtt tttggtgaat
     2161 ttcctgcctg gattgctggc tggctgacga tcatggaatt cttgactgct gtatcgagtg
     2221 ttgcatctgg ttggggagct tatttgaaag gactcctggc taattttggt atcgctatgc
     2281 cgacagcttt gaatggaact tttaaccccg ctgcaggtac ctatgtagac ctcttaccag
     2341 ttctcgtctt gatttttgtg gtaggagtgg tcttgcttaa ttctaaggca gcacttcgtt
     2401 tcaattcagc cttggtagtt ttgaaatttt cagcgttggc tttatttatt ttagtagggt
     2461 tattctttat caaaccagaa aactggtcga acttctctcc ttttggtttt ggtgccattt
     2521 atggtggaca ggcaggaatt atggcaggag cgtctctcat gttctttgcc ttcctgggat
     2581 ttgagtctat ttcattagca attgatgagg tgaaaaaacc tgagaagaat gtaccaaagg
     2641 gaatcgtgtt gtctctatcc attgtaacaa ttctttatat tgtagtgaca ttagtcttga
     2701 ctggtatggt tcactatact aagttgaatg tggcagatgc ggtagccttt gcacttcgag
     2761 aagttggatt ggattgggca gcgagttata tttctattgt tgcaatccta acattgatta
     2821 cagtatgtat ttcgatgacc tacgccttgt cgagaatggt ctattccatc agtcgtgatg
     2881 gtttgctacc gaaatcattg agccgattga cacaaacaag caaggttcct aaaaatgcga
     2941 ccattcttgt ggggattttt gcagccattt gtgcaggtat tttcccacta gcaagtattg
     3001 cttccttcct caatatctgt accttagctt atttgattat gctggcgttg ggtattattc
     3061 gtctgcgtca agtcgaaggt ttgccaaaag atggacaatt taaaacacct cttgttccct
     3121 tgttacctat cctctcgatt atcatttgtc tttcatttat gttccagtat agtttagata
     3181 cttggttagc attcggagta tccgtggtta ttggaatttt gatttacttc tgttatggct
     3241 atcgtcattc ggaagcaaaa taat
//
LOCUS       DAZQCJ010000143         3209 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000143 DAZQCJ010000000
VERSION     DAZQCJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.145"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            59..3142
                     /locus_tag="ACHU66_002019"
     CDS             59..3142
                     /locus_tag="ACHU66_002019"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922092.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
                     /protein_id="HFR3390273.1"
                     /translation="MNIQERFSLRKSAVGLVSVSLLCAIYTSTVAADTVVTGVNEIIE
                     ESQVKDEVSIESEKNESLDGSNIEIVEEIADNIPSPVIAEGEVAVEMKVDRGTENVVS
                     RNDTEVTTSEQNQIEVTETKEILNQTSYQTESGEQRQIIWAHGITPPAMEQSGGFVKE
                     KYGDYLNYTAPFKAGKGYYDTNKSLNASFIDLNLCFAAVSSNMVHWWLEQNSSYVERY
                     LKEKKGTVNVGENYAITDLRRYIDSFQDQQNSRVFDMFKTYYGYRTNGFVSDALVDLF
                     INGYKPKVQGGVNLEDSQLVPDSRGGFFYDVFKEKKLTNRIFSGSYERFGEDVRTVLE
                     SKGLLGLTYRTLGYATHIVTVWGAEYDNQGKIRAVYITDSDDQQEQIGLKRMGITRDA
                     SGNPRLNNHVKNNSAGALLDYVHTIRLGQDLWEEYFNPFAKAKEIASQILADRKKALV
                     LSIQGQSELPESMRLIYLEKLNNLYNQGILSIQKTESSEMLSGALENGLNSLKSLDFP
                     ISEVGNALAPDLSVGDRSTVSDVDSLSSQETSSTNLEADTENAGIIADGTNQLHFPVE
                     AQTTSSVEAEGDNVFEQEADTLPIIIENKDEFGSELSGNMQTSETDSLVVAVEEDVKN
                     DEVDQVEKLLESEKVENQSSELLSDTLIVEGANDKEEDRVEAVVSEQPDSIPHQNVEI
                     SPVEPMNVETESVVTPINDAATPHGFPMYIDNSVTESVAIPLEKDSIQAGETEIAEPT
                     SSESTSVEAELVDNSEIHSATSSVTPRGSSAYADSSTTESVATLLEKDSIQAGETEIA
                     EPTSSKSTNVEAASVDNSEIHADTSLTAVSSVNLDNPVIEPVAIPLIGSKRDTNAEVE
                     VSSLSKREVRKPNTEGLISVQSKVIKKELLESSLVEAGSPLLEATIAQSSNSNSTEIG
                     MSYQNTVLLESNNTERQVSKAEIVIEHKETELVETVSSASEPVVLVENISQTSNNTIE
                     SGKNMGVQSQAGAKQILGIEQSSKVSTPTSRQIMGVGLLTLVLGSALGLLKKRRK"
BASE COUNT         1073 a          547 c          698 g          891 t
ORIGIN      
        1 aaaataaata agagtaagaa taacttgttc aaagaatata aatataaacg gaggaaagat
       61 gaacattcaa gaacgatttt ctttgagaaa atccgcggtt ggcttggtct cagtctcttt
      121 gctatgtgct atttatacat ccactgttgc tgccgataca gttgttacag gagtgaatga
      181 aataattgaa gaatcacaag tcaaggatga ggtatctatt gaatcagaaa aaaatgaatc
      241 cctagatggt tctaatattg aaattgtaga ggaaatagca gacaacatcc catcacctgt
      301 tatcgctgaa ggggaagtag cggtagagat gaaagttgac agagggaccg agaatgtagt
      361 ttctagaaat gatacagaag ttacgacgag cgagcaaaat cagatagagg ttactgagac
      421 aaaagaaatt ttgaatcaga ccagttatca aacggagagt ggcgagcaac gacaaattat
      481 atgggcccat ggaattactc ctcctgcaat ggaacaaagc ggtggttttg taaaggaaaa
      541 gtatggagac tatttaaact atacagcgcc atttaaggca ggaaaaggct attatgatac
      601 caataaaagt ctcaatgctt catttattga ccttaaccta tgttttgcag ccgtgtcttc
      661 caacatggta cattggtggt tggaacagaa tagttcctat gttgagcgat atctcaaaga
      721 aaaaaagggt acagtaaatg ttggggaaaa ctatgcaata acggacctac gtcgctatat
      781 tgattcgttc caggatcagc aaaatagtcg agtctttgat atgttcaaaa cttactacgg
      841 ttatcgtaca aatggttttg tgtcagatgc cctagttgac ttgtttatta atggatataa
      901 acctaaggta cagggtggtg tcaatctgga agatagccag ttagtaccag atagtagggg
      961 tggctttttc tacgacgttt tcaaagagaa aaaactgaca aatcgtattt ttagtggtag
     1021 ctatgagcgt tttggtgagg atgttcgaac tgttttggag agcaaaggat tactcggtct
     1081 aacttataga acattaggct atgcaacgca tattgtgacg gtatggggtg ctgagtacga
     1141 taatcaaggt aagattaggg ctgtctatat cactgattcc gatgatcaac aagaacaaat
     1201 tggtttgaag cgtatgggaa tcactcgtga tgcttctgga aatccgcgtt tgaataatca
     1261 tgtgaaaaat aattcagctg gggcgctttt ggattatgtc catacaatcc gtcttggtca
     1321 agacttatgg gaagaatatt tcaatccgtt cgcaaaagcc aaagaaatag ctagtcagat
     1381 actagctgat agaaagaagg cgttggttct gtctattcaa ggacaatctg aattgccaga
     1441 atcaatgcgg ctgatttatc ttgaaaaact aaataatctc tataatcaag ggattctatc
     1501 tattcaaaag acagaaagtt ctgagatgct aagtggtgca ttggaaaatg gtttaaatag
     1561 tttaaagagt ttagattttc ctatttcaga agttggaaat gctttggcac cagatttatc
     1621 agtaggtgat cgctcaacgg tttcagatgt tgattctcta tcatctcaag aaacaagttc
     1681 cacaaatttg gaagcagaca cagagaatgc aggtattatt gcagatggta ccaatcaatt
     1741 gcattttcca gtggaggccc aaacgacatc ttcagtagag gctgagggag ataatgtttt
     1801 tgaacaagag gcagatacat taccaataat tattgaaaac aaggatgaat ttggttcaga
     1861 actatcagga aacatgcaaa cgtcagaaac ggattcgcta gtagtagctg ttgaagaaga
     1921 tgtgaaaaat gatgaggtag accaagttga aaagcttctt gaatcagaaa aagttgaaaa
     1981 tcagagttcg gaacttctgt cagacaccct aatcgtagag ggtgcaaatg acaaagaaga
     2041 agatagagtg gaggcggttg tttctgaaca accagactca ataccacatc aaaatgtaga
     2101 aatctctcct gtagaaccaa tgaatgtcga aactgaatct gtggtcactc ctattaatga
     2161 tgcagctact cctcatggtt tcccgatgta tatagataat tccgtaactg aatctgtagc
     2221 tattccactt gaaaaagact ccattcaagc cggagagaca gaaattgcag aaccaacctc
     2281 gagcgaatca actagtgttg aagctgaact tgtcgacaat tctgaaatcc attcagctac
     2341 ctcttcagtt actccccgtg gttcctcggc atatgcagat agttccacaa ctgaatctgt
     2401 agctactctg cttgaaaaag actccattca ggctggagag acagaaattg cagaaccaac
     2461 ctcgagcaaa tcaactaatg tcgaagctgc atctgtcgac aattctgaaa ttcatgcaga
     2521 tacctctcta actgctgttt catcagtcaa tctggataat ccagtgattg aaccagtagc
     2581 tatccccctt atcggttcta agagggacac gaatgcagaa gtggaagttt cttcattatc
     2641 gaaaagagag gttagaaaac caaatactga agggctaatc tctgttcaat caaaagttat
     2701 taagaaagaa ttgctagaat caagcttagt agaagcaggg tctccattgc tagaagccac
     2761 cattgctcag tcttcaaact caaatagtac tgagataggt atgagctatc agaatactgt
     2821 gttattagag tctaataata cagagcgtca ggtgtctaaa gcagaaattg ttatagaaca
     2881 caaggagaca gagttagttg aaacggtttc atctgcttct gagcctgtag tgctcgtaga
     2941 aaatatctca caaacctcaa ataatactat tgaatctggt aagaatatgg gagttcaatc
     3001 tcaagcaggt gcaaaacaaa ttttaggcat agaacaatct tcgaaagtaa gtacacctac
     3061 ttcaagacag attatgggag tcggtctatt gactcttgtt cttggtagtg ctttaggttt
     3121 gttaaagaaa agacgtaagt aaaagaagta tcgtcattta gtttttcttt tattacttcg
     3181 ttaactcgcc ttgcctaact ccagttatg
//
LOCUS       DAZQCJ010000144         3048 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000144 DAZQCJ010000000
VERSION     DAZQCJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.146"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            253..474
                     /gene="relB"
                     /locus_tag="ACHU66_002020"
     CDS             253..474
                     /gene="relB"
                     /locus_tag="ACHU66_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFR3390274.1"
                     /translation="MSTVTIRLNQEEEVFFKSYAQLTGQSLSSLFKKALERDIEDEYD
                     LKIYHQAYDEYKADPETISHADFKKELGL"
     gene            476..736
                     /locus_tag="ACHU66_002021"
     CDS             476..736
                     /locus_tag="ACHU66_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR3390275.1"
                     /translation="MYHLEYSKKAQKQIKKLDRQIQRLLFAWIDKHLEGTDNPRANGK
                     GLTGNHANEWRYRIGDYRLICDIQDDKLVILALEFGHRKDVY"
     gene            903..1544
                     /locus_tag="ACHU66_002022"
     CDS             903..1544
                     /locus_tag="ACHU66_002022"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFR3390276.1"
                     /translation="MIEWIFFDLGSTLLDEEAAYGYYIDKCVKKLESLDIEVSSDSYK
                     KKMVEYAHKSLDPIRATWHYFAPTEPRPLWTNEGVSLYPETIDALEKLSQNYRLGIIA
                     NQSSSIRELLKEWGIESYFQLIILSEEVGLSKPNTAIFTLALQKTNIPADRVVYVGDR
                     YDNDILPAKSLGMWTVRILTGFGKHASENEKLKSDWVIPSLQEITNIFEQTKN"
     gene            complement(1577..1762)
                     /locus_tag="ACHU66_002023"
     CDS             complement(1577..1762)
                     /locus_tag="ACHU66_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390277.1"
                     /translation="MNPVDLFNQVKELIANKDFDGAKQFIEENKDQFGEYLEQAKGLL
                     SGSEGVNGLLDKVKGLF"
     gene            complement(1826..2830)
                     /locus_tag="ACHU66_002024"
     CDS             complement(1826..2830)
                     /locus_tag="ACHU66_002024"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFR3390278.1"
                     /translation="MAIYFGTYTKRESKGIYKANFDSETGQLSNLELVTEEPNPTYLA
                     FDKAGHLYSVGAENGLGGIAAFNAEYTLLNHVVSEGAPLCYVAVDEARDLVYGSNFHK
                     GQLLVYKRLADGSLELADVAQHEGSGPHENQASAHVHFSDLTPDKFLVTCDLGCDQVV
                     TYKVSDQGKVEKIATYQAAPGSGPRHIVFHPVAKIAYLICELNSTIEVLIYDGWGQFE
                     HLQTVSTLPEDHTGFNGTAAIRITKDGKFIYGSNRGNDSIAVYKTLADASLELVEIVP
                     TNGKTPRDFTLSPDEKHLIVVHQDSDNATVFERDAENGCLTELSHDFYVPEAVCVTFL
                     "
BASE COUNT          954 a          509 c          640 g          945 t
ORIGIN      
        1 caagtctgga aatttccaga cttgtttttc gtagtacagt gtactaattt atccccgagc
       61 tagtatagtg agggatatag taagaacaag tctgaaaata ttcggacttg ttctttgact
      121 ttatatgatt taagggctgt aaagccttga tataaggcac tttggggcat catcccaagg
      181 tacttttttg atagattgac agatacttta aaaatgtgtt acaatgtaac acaaagaagc
      241 gaggtgaata tcatgagtac agtgacaatc agattaaatc aagaagaaga agtattcttt
      301 aaaagctatg cacaattaac aggtcaaagt ctttctagcc tttttaagaa agctttggag
      361 cgtgatattg aagatgagta tgatttaaaa atctatcatc aagcttatga tgagtacaaa
      421 gcagatccag aaacaattag tcatgctgat tttaagaaag agctaggatt atagcatgta
      481 tcatttagag tattctaaaa aggctcaaaa gcaaattaag aaattagata ggcaaattca
      541 gaggttgttg tttgcttgga ttgataaaca cttagaaggg actgataatc cacgcgccaa
      601 tggtaaaggt ttgacaggca atcatgctaa cgagtggcgt tatcgtatcg gagattaccg
      661 actgatttgc gatattcaag atgataagtt ggttatttta gcacttgagt ttggacatcg
      721 taaagatgtc tattgatgcg tgaaaatcta tgacaagata ggtcctaact atttttaaga
      781 atgaatagaa aactagactg aaattcctag ggaattttct ttgccttgat attgataaaa
      841 tgatattcta aaaaggcaga tcaacttaag tattctcatt ttgagaagag atggagagtg
      901 ggatgataga gtggatattt tttgatttgg gttcgacgct tttagatgaa gaggctgctt
      961 atggttatta tatagacaag tgtgtgaaga agttagagtc tctggatata gaggtttcat
     1021 cagattctta taaaaagaaa atggtggagt atgctcataa gtcgctagat ccaattcgtg
     1081 cgacctggca ctattttgca ccgaccgagc cacgtccttt atggacaaat gagggtgtga
     1141 gcctgtatcc tgaaacaata gatgctctag agaagttatc acaaaactat cgattgggga
     1201 ttattgccaa tcagtctagt agtattagag aactgcttaa agagtgggga attgagtctt
     1261 attttcaact catcatcctc tctgaagagg ttggtttatc taaaccaaat acagctattt
     1321 tcacacttgc cttgcaaaaa acaaacatcc ctgcagatag agttgtctat gtcggggata
     1381 ggtatgataa tgatattctg cctgctaaat ctttgggaat gtggactgta cggatactga
     1441 caggttttgg gaagcatgcc agtgaaaatg aaaaattgaa gagcgattgg gtaattcctt
     1501 cattgcaaga gataacaaat atttttgaac aaacaaaaaa ctagactgaa tattctcagc
     1561 ctagttttta catgtattag aacaagcctt taactttatc taaaagaccg ttaacacctt
     1621 cagaacctga taacaagcct tttgcctgtt ccaagtattc accgaattgg tccttgtttt
     1681 cttcgataaa ttgttttgca ccatcaaagt ctttgtttgc aatcaattct tttacttggt
     1741 taaaaagatc tactggattc atgttgaaat cacctcctat gagactatta aaccaaaact
     1801 gtacggattt cacaattgaa acgacttata aaaatgtaac gcaaactgct tcaggaacat
     1861 agaaatcgtg tgaaagctca gtcaaacagc cattttcggc atctctttcg aatacagttg
     1921 cattgtcaga atcttgatgg acaacaatca aatgcttttc atcaggacta agcgtgaaat
     1981 cgcgaggagt ttttccattt gttgggacga tttcaactaa ttctaagctg gcatcagcaa
     2041 gtgttttgta gactgcgata gagtcgtttc cgcggttgga tccataaata aatttaccat
     2101 cctttgtgat gcgaatagcg gcagtaccgt tgaaaccagt atggtcttct gggagagtag
     2161 agacagtttg taggtgttca aattgtcccc agccgtcata gataagcacc tcaatagttg
     2221 aattgagttc acagatgaga taggcgattt ttgcgacagg atggaagaca atgtgacgtg
     2281 gaccacttcc tggcgcagcc tgataggttg ctattttctc tacttttcct tggtcagata
     2341 ccttataggt taccacttga tcacatccta aatcacaggt tactaaaaat ttatcgggcg
     2401 tcaaatcaga aaagtgaacg tgggcggaag cttggttttc gtgcggccca gatccttcgt
     2461 gttgagcgac atcagctaat tctagcgaac catccgccaa acgtttataa acaagtagtt
     2521 gccctttgtg gaaattggaa ccataaacta aatctcttgc ttcatctaca gcgacatagc
     2581 aaagtggtgc gccctctgat acaacatggt tgagtagagt atactcagca ttaaaggcag
     2641 cgatacctcc tagaccattt tcagcgccga cggagtatag atgtccggcc ttatcaaagg
     2701 caaggtaggt agggttaggt tcttcagtga ccaattctag attgctaagt tgtcctgttt
     2761 cagagtcaaa atttgcttta taaattcctt ttgattcacg ttttgtatag gtaccgaaat
     2821 agatagccat aggattctcc tttttggaaa tgattgataa aattatacca tgtttttctg
     2881 agaaagtcgt ataaattctg tagttgattt ttacaaaaat cctttctcca tgttacaata
     2941 gaaaaaaaga ctaggagtgt attagatgga ggataagcat caaaaattta gtctgacttg
     3001 gtttttccgc ttgtttttaa atagccaagc ggtgacattt ttattggt
//
LOCUS       DAZQCJ010000145         2801 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000145 DAZQCJ010000000
VERSION     DAZQCJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.147"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            10..702
                     /locus_tag="ACHU66_002025"
     CDS             10..702
                     /locus_tag="ACHU66_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFR3390279.1"
                     /translation="MFSPKRLKEKRTELGLSQSEIARQLEISRMSYSAWERGKTKPNH
                     KNLSLLATILKVDESYFESEYSIVQNYLLLTDENKLAAEDYVEQLLTSQSIAENVVSL
                     YSYQVLSDIQLSAGPGEGLYDEFETETVYSEDEYTGFDIATWISGNSMEPVYKDGEVA
                     LIRSTGFDYDGAVYALNWNGSLYIKKLYREEDGFRMVSLNEDYTEKFIPYEDEPRIVG
                     LVVGHFMPVIGG"
     gene            705..839
                     /locus_tag="ACHU66_002026"
     CDS             705..839
                     /locus_tag="ACHU66_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390280.1"
                     /translation="MARQFDDMIYIKGNRIILALDQKDYDVTDHLTELVEELAKLKSR
                     "
     gene            845..2260
                     /locus_tag="ACHU66_002027"
     CDS             845..2260
                     /locus_tag="ACHU66_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFR3390281.1"
                     /translation="MSYIDYSKEPRSDIAFVDMKSFYASIECVERGLHPLHTSLCVMS
                     RADNTSGLILASSPMFKKIFGKGNVGRAYDLPFDVHTRKFNYYRASISGLPTDVKFVS
                     YIENWAKRTFIVPPRMDLYIQKNLEIQKVFQNYADPTDILPYSIDEGFIDLTSSLNYF
                     VEDKTLSRKDKLDLVSAKIQHDIWEKTGVYSTVGMSNANPLLAKLALDNEAKSTATMR
                     ANWSYEDVETRVWNIPKMTDFWGIGSRTEKRLNKLGIYTIKELANCDPSILKKEFGVI
                     GVQHWFHANGIDESNVHEPYRPKAVGIGNSQVLHKDYTRQSDIELVLREMAEQVAIRL
                     RKRHKKATVVAINVGYSNFENKKSINVQRKINPTNRTLVFQDEVISLFRSKYDGGAVR
                     SIAVRYDGLVDENFAVISLFDDFEESEKEEKLETTIDTIRDRFGFLAVQKASSLLENS
                     RAIARSKLVGGHSAGGLEGLK"
     gene            2257..2607
                     /locus_tag="ACHU66_002028"
     CDS             2257..2607
                     /locus_tag="ACHU66_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390282.1"
                     /translation="MIDRSYLPFQVAREYQDRKMAKWMGFFLSEHTSGLEQERNRIDY
                     STELTLADKLLLLNQLYSHELNGILTVPGKIYIGIVKNLTFQSVSFETKEGYISIQIS
                     EILTIDLEVEYESA"
     gene            2594..>2801
                     /locus_tag="ACHU66_002029"
     CDS             2594..>2801
                     /locus_tag="ACHU66_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFR3390283.1"
                     /translation="MNQLEFNKNQLQMDYFSDSYRKFEEDFYRYSKLAVPLTFLTDDL
                     MNSMASSGINYFVLNKEKSKDNRDH"
BASE COUNT          940 a          489 c          552 g          820 t
ORIGIN      
        1 ggagtaacca tgttctctcc aaaaagacta aaagagaaaa gaacggaact aggactatct
       61 caatctgaaa tcgcacgaca acttgaaatt agtcgcatgt catatagtgc ctgggaacgt
      121 ggaaaaacca aaccaaacca taaaaatctt tctctgcttg ctactatttt gaaagttgac
      181 gagtcctatt ttgaatctga atattctatc gttcaaaact atcttttgtt aaccgatgaa
      241 aacaaattgg ctgctgaaga ttacgttgag caactactaa catctcaatc tattgcagaa
      301 aatgttgtgt cactatactc ctatcaagtg ctttcagata tccaattatc tgctggtcct
      361 ggtgaagggc tgtacgatga atttgaaaca gagactgtct attcggaaga tgaatatacg
      421 ggctttgata tagcgacatg gatttcagga aactcaatgg aacctgttta taaagacggc
      481 gaagtcgcct taattaggtc tactggtttt gattatgatg gtgctgtcta tgccttaaat
      541 tggaatggct cactctacat aaagaaactc tatcgtgaag aagatgggtt cagaatggtc
      601 tccctgaatg aagactatac agagaagttc atcccttatg aagatgagcc aagaattgtt
      661 ggtctagttg tgggccactt tatgcctgtg attggaggtt agcaatggca agacagtttg
      721 acgatatgat ttacattaaa ggaaatcgca ttattctagc actcgaccaa aaagattacg
      781 atgtaacaga ccatctaaca gagcttgttg aagagttagc aaaattgaaa tctaggtgaa
      841 cactatgagc tatatcgact attcaaagga accacgtagc gatattgctt ttgtcgatat
      901 gaaatctttc tatgcgtcca ttgaatgcgt ggagagaggg cttcatccgc tccatacatc
      961 tctttgtgtt atgagtaggg ctgataatac ttctggcctc atactagctt cttctccaat
     1021 gttcaaaaaa atttttggta aaggaaatgt cggtagagct tacgacctcc cctttgatgt
     1081 ccacacacga aaatttaact attaccgtgc aagcatttct ggactaccaa cagatgtcaa
     1141 gttcgtctca tatattgaaa actgggctaa gcgtaccttt atcgtgccac caagaatgga
     1201 cttgtatatc caaaaaaatc tagaaatcca aaaagtattt caaaattatg ctgatccaac
     1261 agatattctc ccctattcaa ttgacgaagg atttattgac ctaacttcat cactaaatta
     1321 ttttgttgaa gataagacgc ttagtcgtaa agataaatta gatctagtat cagcaaaaat
     1381 acagcacgat atttgggaaa agactggagt atattctaca gttggtatga gtaatgccaa
     1441 tccacttcta gccaagctgg cacttgacaa tgaagcaaaa agtactgcta ccatgcgagc
     1501 aaattggtcc tatgaagatg ttgaaacaag ggtctggaac attccgaaaa tgactgactt
     1561 ttggggcatt ggttcacgaa cagaaaaaag attgaataaa cttggtatct atactatcaa
     1621 ggaattggcc aactgtgacc catctatctt gaaaaaagaa tttggtgtga ttggagtaca
     1681 acattggttt catgctaatg gtattgatga aagcaatgta cacgaaccct atcgccctaa
     1741 agctgtcggg attggaaatt cgcaagtgct ccacaaagat tacactcgtc aaagcgatat
     1801 agaacttgta cttagagaga tggcagagca agttgccatt cgcttacgta agagacacaa
     1861 aaaagcaacc gtagttgcca taaatgttgg atattcaaat tttgaaaaca agaaatccat
     1921 caatgttcag agaaaaataa atccaaccaa tcgaacactt gtttttcagg acgaagttat
     1981 ttcacttttt cgctcaaaat atgatggtgg tgcagttaga agtattgctg tgagatacga
     2041 tggattagtg gacgaaaact ttgcagttat ttcacttttt gacgactttg aagaaagcga
     2101 aaaagaggaa aaattggaaa caactattga taccattcgt gatcgatttg gatttcttgc
     2161 agtacagaaa gctagttctt tattagaaaa ttcaagggca attgctcgaa gtaaacttgt
     2221 cggcggtcat tccgctggcg gattggaggg attaaaatga ttgaccgtag ctatctacca
     2281 ttccaagtag ctagagaata ccaagataga aaaatggcaa aatggatggg atttttcttg
     2341 tcagagcata cttcaggttt agaacaagaa cgcaatcgta ttgattattc aactgaattg
     2401 acattagctg ataaactact cctactcaat caactatata gtcatgagtt aaatggtatt
     2461 ctgacagttc ctggaaaaat ttacattgga atagtaaaaa atttgacatt ccaatctgtt
     2521 tcgtttgaaa ctaaagaagg ctacatcagt attcaaatct cggaaattct tacaatagat
     2581 ttggaggtgg agtatgaatc agcttgagtt taataaaaat caattacaaa tggattattt
     2641 tagtgatagc taccgaaagt ttgaagagga tttctatcgc tactcaaaat tagccgttcc
     2701 acttaccttt cttacagatg atttgatgaa ttcaatggct tcatcaggta ttaactattt
     2761 tgttttaaac aaagaaaaat caaaagacaa ccgagatcat t
//
LOCUS       DAZQCJ010000146         2782 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000146 DAZQCJ010000000
VERSION     DAZQCJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.148"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            168..1310
                     /gene="tgt"
                     /locus_tag="ACHU66_002030"
     CDS             168..1310
                     /gene="tgt"
                     /locus_tag="ACHU66_002030"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR3390284.1"
                     /translation="MTDVPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTMSPEELKAMGSGIILSNTYHLWLRPGDELVARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNISEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFPGYSI
                     GGLAVGETHDEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSRGRLVVKNAQFAEDFTPLDPECDCYTCKNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLINLMKNVRQAIMDDNLLEFRQDFMEKYGYGKNGRNF"
     gene            1355..1489
                     /locus_tag="ACHU66_002031"
     CDS             1355..1489
                     /locus_tag="ACHU66_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390285.1"
                     /translation="MKIKIKLGDADADRTEVHQIKSTTSDFDFRRVSVTPSQDLVRYK
                     "
     gene            complement(1643..2728)
                     /locus_tag="ACHU66_002032"
     CDS             complement(1643..2728)
                     /locus_tag="ACHU66_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFR3390286.1"
                     /translation="MSKLHHVKSYLEANKMDLAIFSDPVSIYYLTGYHSDPHERHMML
                     FVMPDHDSLLFLPALDVERAVATVDFPVAGYMDSENPWQIIKSKLPQKSFSVICAEFD
                     NLNLTRYHGLQSIFSQPFSDITPLINTMKLIKSRDEIEKMLVAGEFADKAMQVGFNNI
                     SLNVTETDIIAQIEFEMKKQGISKMSFDTMVLTGDNAANPHGIPSTNKIENNALLLFD
                     LGVEALGYTSDMTRTVAVGKPDQFKKDIYNLTLEAHMAAVNMIKPGVTAGEIDYAARS
                     VIEKAGYGEYFNHRLGHGLGMSVHEFPSIMEGNDLVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGYVTKNGFEVFTKTPKELLYFEG"
BASE COUNT          828 a          557 c          592 g          805 t
ORIGIN      
        1 gctgaaataa gcgagaacat taaagacatt ctgatggatt agaccatgca agattgagaa
       61 actgtatttc caggtaaagt acttttatgg atgatatgaa agagctgaac tgtgaatcca
      121 gaactaatat cctaggactg tttttcctag cgggaggtaa ataaaacatg acagatgttc
      181 caatcaagta tcgcttgatt aagaaagaaa agcacacagg tgcacgtttg ggcgaaatca
      241 ttaccccaca cgggaccttt ccaactccga tgtttatgcc agttggaact caggcaacgg
      301 taaaaaccat gtcgcccgaa gaattgaagg cgatgggttc aggaattatc ctatctaata
      361 cctatcattt gtggcttcgt ccaggtgacg aattggtggc gcgtgcaggc ggtttgcaca
      421 aatttatgaa ttgggatcaa ccgattctga ctgactcagg tggtttccag gtttattctt
      481 tggcggatag tcgcaatatc tcagaagaag gtgtgacctt caaaaaccat ctcaatggta
      541 gtaagatgtt cctgtcacct gaaaaagcta tctctattca gaacaatctt ggctctgaca
      601 ttatgatgag ctttgatgag tgtccgcagt tctaccagcc ttatgactat gtaaaaaaat
      661 ccatcgaaag aacaagtcgt tgggcagaac gtggtctcaa ggctcaccgc cgtccgcatg
      721 accaaggttt atttggtatt gtccaaggtg ctggttttga agatcttcgt cgccaatcag
      781 cccatgattt ggtcagcatg gattttccag gatactctat tggaggattg gctgttggtg
      841 aaacacacga tgaaatgaat gcagtactgg acttcacgac ccctctttta ccggagaata
      901 aaccacgcta tctcatgggc gttggagcac cagatagctt gattgatggc gtaattcgtg
      961 gggttgatat gtttgactgt gtattaccaa cacgcattgc gcgtaatgga acctgtatga
     1021 ccagtcgtgg acgtcttgtg gtgaaaaatg ctcagttcgc tgaggacttt acaccgttag
     1081 atccagagtg cgattgctac acatgtaaaa actatacacg tgcgtatctc cgccatttac
     1141 ttaaggccga cgagacattt ggtattcgtt tgacaagtta tcacaacctc tacttcttga
     1201 ttaatttgat gaaaaatgtc cgtcaagcaa tcatggatga caaccttttg gaattccgtc
     1261 aagactttat ggaaaaatac ggttacggta aaaacggacg taatttttag ataaaacagc
     1321 cttctttgtg gacttagcct tgttctcata ctcaatgaaa atcaaaatca aactaggcga
     1381 cgcagatgca gatagaactg aagttcatca aatcaagtca acaacgtctg attttgattt
     1441 tcgaagagta tcagttacac cttctcaaga tttggttaga tacaaataaa taaagcatac
     1501 gattatgaaa cccctcggta acataatcgt atgcttttat tttaatactt ggcttcacag
     1561 cacttctatt ttaatgttct ttgaagatta aaatgaacag ttgtcaacga gctttgatga
     1621 gtgaaaaaac gattggtccc cattaccctt caaaatacag taattctttc ggagtctttg
     1681 tgaatacttc aaagccattt ttggtaacgt aaccacaatc ctcgatacga acaccaactt
     1741 ttccagggat gtaaatacct ggttcgacag agaaacacat accttcttcg atgactaaat
     1801 cattgccttc cataattgat gggaattcat ggacactcat tcccaaacca tgaccgagtc
     1861 ggtggttgaa gtactcgcca taacctgctt tttcaatcac agagcgagcg gcatagtcaa
     1921 tctcaccagc agtcacacca ggcttaatca tattgaccgc ggccatgtga gcctccagag
     1981 tcaagttgta aatatctttt ttaaactgat caggtttacc gacagcgaca gtccgtgtca
     2041 tatcagacgt atagcccaaa gcttccacgc ctaggtcgaa caatagcaga gcgttattct
     2101 cgattttatt tgttgaggga ataccgtgtg gatttgcggc attgtcgcct gttaagacca
     2161 tggtgtcaaa gctcattttt gagatgcctt gtttcttcat ttcaaattca atctgagcaa
     2221 taatatcagt ttcagttacg ttgagggaga tattgttaaa accaacctgc atagccttat
     2281 ccgcaaactc tccagcaacc agcatttttt caatttcatc acgcgacttg atcaatttca
     2341 tagtattaat cagaggtgta atgtctgaaa atggttggct aaagatagac tgaagaccat
     2401 ggtagcgggt taaattcaaa ttatcaaatt ctgcacagat tactgagaaa gatttctgtg
     2461 gcaatttgct cttgataatc tgccatggat tttctgagtc catatagccg gctacaggaa
     2521 agtcaacagt tgcaactgca cgttctacat ccaaggcagg gagaaagagt agtgagtcgt
     2581 ggtcaggcat aacaaagagc atcatgtggc gctcatgagg atcgctatga tagccagtca
     2641 gataatagat gctaactgga tcagagaaga tggccaagtc cattttgttg gcttctaagt
     2701 aggatttaac atgatgtaat tttgacatag tggagacctt tctagcatgt agtggtaaaa
     2761 aaaagtgaaa ttttcgttta tt
//
LOCUS       DAZQCJ010000147         2742 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000147 DAZQCJ010000000
VERSION     DAZQCJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.149"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            34..1197
                     /locus_tag="ACHU66_002033"
     CDS             34..1197
                     /locus_tag="ACHU66_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR3390287.1"
                     /translation="MRIKDKIKKNGQKVYYASVYLGVDQLTGKKARTTVTATTQKGVR
                     VKARDAINAFAANGYTVKDKPTITTYKELVKVWWDSYKNTVKPNTRQSMDGLVRVHLL
                     PVFGDYKLSKLTTPIIQQQVNKWADKANTGQKGAFTNYSLLHNMNKRILKYGVSMQVI
                     TYNPANDVIVPRKQQKEKAGVKYLDNKELKQFLDYLDTLDQSNYENLFDVVLYKTLLA
                     TGCRIGEALALEWSDIDLDNGIVSINKTLNRFQEINSPKSSAGYRDIPIDNATLLMLK
                     QYKNRQQVQSWQLGRSETVVFSVFTEKYAYACNLRQRLNKHFKAAGVTNVSFHGFRHT
                     HTTMMLYAQVSPKDVQYRLGHSNLMMTENVYWHTNQENAKKAVSNYETAINSL"
     gene            complement(1357..2664)
                     /gene="eno"
                     /locus_tag="ACHU66_002034"
     CDS             complement(1357..2664)
                     /gene="eno"
                     /locus_tag="ACHU66_002034"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-displayed alpha-enolase"
                     /protein_id="HFR3390288.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYLGLGTQKAVDNVNNVIADAIIGFDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYTYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPSFKEGLRWGAEVFHALKKILKARGLVTAVGDEGGFAPKFEGTEDG
                     VETIIEAIEAAGYEAGENGIMIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGKRVQLVGDDFFVTNTDYLARGIK
                     EGAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAVYKGLNSFYNLKK"
BASE COUNT          861 a          578 c          563 g          740 t
ORIGIN      
        1 cttgcctgct gatggaaaga aaggtacaaa tacatgagaa tcaaagataa aatcaaaaag
       61 aacggtcaaa aagtttacta tgctagtgtt tatctaggcg ttgaccaact aacgggcaag
      121 aaagcccgta caactgttac agcaaccacc caaaaggggg ttagggtaaa agcgcgtgat
      181 gctatcaatg cttttgctgc taatggctat acagttaaag acaagccgac aattacaacg
      241 tacaaggagc ttgtaaaagt ttggtgggat agttacaaga atacagttaa gccaaatact
      301 cgccaatcaa tggatgggtt ggttagagtg catctattgc ctgtatttgg cgattacaag
      361 ctatctaaac tcactacgcc tattatccaa caacaagtaa acaaatgggc tgacaaggct
      421 aatacagggc aaaaaggggc gtttactaac tactccttac tccataacat gaataagcgt
      481 attttgaaat acggtgtttc tatgcaagtg ataacgtata acccagctaa tgatgttata
      541 gtgccacgta agcaacaaaa agaaaaagct ggtgtaaaat acctggacaa caaagaacta
      601 aaacaatttc ttgattatct cgatacctta gaccaatcaa actatgaaaa cttgtttgat
      661 gttgtcctat acaagacttt gttagctact ggttgccgta tcggggaggc actagctctt
      721 gaatggtctg atattgacct agataacggt attgtcagca tcaacaagac actcaaccga
      781 tttcaagaga taaactctcc taaatcaagc gctggttatc gtgatatacc aatagacaat
      841 gccacattac ttatgctcaa acaatacaag aaccgccagc aagtacaatc atggcaacta
      901 gggcggtctg aaacggttgt attctctgta tttacagaaa agtatgccta tgcttgtaat
      961 ttgagacaac gcctaaataa gcattttaag gctgctggcg tgactaatgt atcatttcat
     1021 ggtttccgtc acacacatac tactatgatg ctctacgctc aagttagccc caaagatgta
     1081 caatacaggc taggtcactc taacttgatg atgactgaaa atgtttattg gcacactaac
     1141 caagaaaacg caaaaaaagc tgtctcaaat tatgaaacag caatcaatag tttataaagg
     1201 gggtcacact ttgtgactgc cctcttacta tacaaaaaat acagcatttc aatcatgggg
     1261 tagtacaaag ggtagtaaat tctaaaaaag caccttggga tgatgcccca aagtgcctaa
     1321 tatcaaggct ttacagccct taaccccata aagagattat tttttcaagt tgtagaatga
     1381 gttcaagcct ttgtagactg caacttcacc aagttgatct tcgatacgaa gcaattggtt
     1441 gtatttagcg atacggtctg tacgtgacaa tgaaccagtc ttgatttggc ctgcgttagt
     1501 tgcaactgcg atgtcagcga ttgttgaatc ttcagtttca cctgaacggt gtgatacaac
     1561 ggcagtgtag ccagcttctt tagccatttc gatagcttca aatgtttcag taagagtacc
     1621 gatttggtta actttgataa ggattgagtt agcagcacct tctttgatac cacgtgcaag
     1681 gtagtcagtg tttgttacga agaagtcgtc accaaccaat tgaacgcgtt tgccaagacg
     1741 ctcagtaaga gctttccagc catcccagtc gttttcatcc ataccatctt cgatagtgat
     1801 gattgggtat ttgttaacca attcttcaag gtagtcgatt tgttctgcag aagtacgaac
     1861 agcagcgcct tcaccttcga atttagtgta gtcgtaaact ttacgctctt tatcgtagaa
     1921 ttcagatgac gcacagtcga aaccgatcat gatgccgttt tcgccagctt cgtaaccagc
     1981 agcttcgata gcttcgatga ttgtttcaac accgtcttca gttccttcga acttaggagc
     2041 gaaaccacct tcgtcaccaa cagctgttac caaaccacga gctttcaaga ttttcttcaa
     2101 agcgtggaat acttcagcac cccaacgaag accttctttg aatgaaggag cgccaactgg
     2161 caagatcatg aattcttgga aagcgattgg agcgtctgag tgagaaccac cgttgatgat
     2221 gttcatcatt ggagttggca atactttagt gttgaatcca ccaaggtaag tgtaaagtgg
     2281 cacttcaagg tagtcagcag cagcacgcgc aacagcgata gaaacaccga ggattgcgtt
     2341 tgcacccaat ttacctttgt taggagtacc gtcaagagcg atcatagcgc ggtcgatagc
     2401 ttgttgatca cgaacgtcaa aaccgatgat agcgtcagca atcacgttgt tcacgttgtc
     2461 aacagctttt tgagtaccaa gaccaaggta acgagatttg tcaccgtcgc gaagctcaac
     2521 tgcttcgtgc tcaccagtag aagctcctga aggaaccata ccacgtccga aagcacctga
     2581 ttcagtataa acttcaactt caagtgtagg gttaccgcgt gagtcaagga cttcgcgagc
     2641 gtaaacatca gtaataattg acattatatt actctccttt gagtttaaaa ttgttacata
     2701 gctatcatac cctaaaagaa gggatttttc aagaaaaaac ag
//
LOCUS       DAZQCJ010000148         2643 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000148 DAZQCJ010000000
VERSION     DAZQCJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.150"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..640)
                     /locus_tag="ACHU66_002035"
     CDS             complement(<1..640)
                     /locus_tag="ACHU66_002035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR3390289.1"
                     /translation="MKRILFISPTTSMDNGAEKSIYYLMKHLIQLGHTVINVTHAIGG
                     EPQDKHEQLYKMIGVKNYYLPTVKWWWPDAPGGEILNREEATSYYRQNVQDIAEIIDQ
                     NEIDLVISNTVNVFQGAIAASCQKVPHFWLIHEFPKNEFAYYADKIDFIEEYSSELFS
                     VTGELNNFLTPLFNKKVHSFISYTEQETKVLKKGNQVRIVSVGRLTEGKNQLE"
     gene            complement(640..>2643)
                     /locus_tag="ACHU66_002036"
     CDS             complement(640..>2643)
                     /locus_tag="ACHU66_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HFR3390290.1"
                     /translation="LSGKYKLLFYILELTKNIAKFFWRIPKYIKRTLLALQRDKQRRN
                     NYSDIKNRYLIYTIYEHQSSLQDYKVIFLEALAKISRDVLIVVNGKLPQADINRLAQY
                     GKVLERENEGYDVAAFRHGIIHTGKEALQQYNQLILVNDTNIGPFRDLEEVFSEFNSD
                     QLDFWGISMGEEQLDFTGYNPYGKIPKHLQSYFVVIENSLLRYEGFYDYWEKLSDTDS
                     RNKAIGKHETVFAKYFYDRGFKYDALIKDTKDSALYIHPLKLLKQGCPLVKYSAFRNY
                     DREQYFWHGLERESEIPDLMEYIAKETDYPIEVVSSILEDFKTRENQSYILIIDGVEN
                     IIPQCTRYRVLNKAEQLRELGYTVRVINNSLVQLQDAQFASHIIIYRAPFNDMLKEIC
                     RAAHIKNRPVYFDIDDLVFDTKFTDELEFTQGLSKREKKGYDTSVLAYKKMLSLCDYA
                     ITSTSKLKDELEQYKNKVILNRNVMSKELVERSLQVKKHSNDNKVKIGYFSGSITHNE
                     NFDLISQALLHLLQKYPQVELHIVGYLDIPKPFQKFKKQIVSHEYVDWRKLPILISQV
                     DINLAPLVTTTFNEAKSEIKWIEAAAVKVVTVASNLGAFEEMIQDGVTGVLADDNEWE
                     SKLERLILEQDLREQIAENAFEFVMNHCTTANRINDFLKEELV"
BASE COUNT          836 a          517 c          365 g          925 t
ORIGIN      
        1 gttctaattg attttttcct tctgttagtc gaccaacaga aacaattcta acttgattgc
       61 ctttttttaa aacttttgtt tcttgctcag tataagaaat aaacgaatgg actttcttat
      121 taaatagtgg tgttaaaaaa ttatttaact ctccagtcac actaaataat tctgaagaat
      181 actcttctat gaagtcaatt ttatcagcat aataagcaaa ctcattttta ggaaattcat
      241 gaattagcca gaaatgggga accttttgac atgaggcagc aattgctcct tgaaaaacat
      301 taacagtatt tgaaataact aagtctatct cattttgatc aataatttca gctatgtctt
      361 gaacattctg gcgatagtag cttgtagcct cttctctatt aaggatttct ccacctggtg
      421 catccggcca ccaccacttg actgttggca aatagtaatt cttaacccct atcattttat
      481 aaagttgctc atgcttatct tgtggttccc ctcctattgc atgagtaaca tttataactg
      541 tgtgtcccag ttgaataagg tgcttcatta gataataaat agacttctcc gcaccattat
      601 ccatactagt cgtaggagaa ataaacaaaa tacgtttcat tagactaact cttctttcaa
      661 aaaatcatta attctatttg ctgtagtgca atgattcatt acaaactcaa aagcattttc
      721 tgctatttgt tctctcaagt cttgttcaag aatcaatctc tccaatttac tttcccattc
      781 gttatcatct gctagaactc cagtcactcc atcttgaatc atctcttcaa atgctccaag
      841 attacttgca actgtcacaa ctttaactgc tgccgcttct atccatttaa tttccgactt
      901 tgcttcatta aaggttgtgg ttacaagcgg cgccaagtta atatcaactt gtgaaattaa
      961 aattggaagc tttctccaat caacatactc atgactcaca atctgttttt tgaatttttg
     1021 gaatggtttc ggaatgtcta aatatcctac tatatgtaat tccacttgcg ggtacttttg
     1081 aagtagatgt agcaatgctt gactaattaa atcaaaattt tcgttgtgtg taattgaccc
     1141 tgagaaatat cctattttaa ctttattgtc gttcgaatgt ttcttaactt gtaaactacg
     1201 ctcaactaat tctttagaca tgacgttgcg attcaagata accttgtttt tatattgttc
     1261 aagctcgtct ttcaatttcg aagtcgatgt gatagcatag tcgcagagtg aaagcatttt
     1321 tttgtaagct aaaacacttg tatcatagcc ttttttctct ctcttgctca aaccttgggt
     1381 aaattcaagt tcatctgtaa attttgtatc aaaaactaaa tcatcaatat caaaataaac
     1441 aggtctgttc tttatgtgtg ctgccctaca tatttctttc agcatatcat tgaatggagc
     1501 acgataaata ataatatgac tagcaaactg tgcatcctga agttgtacaa gagaattatt
     1561 tatcactcga actgtataac caagctctcg caattgctca gccttattta acacacgata
     1621 acgtgtacat tgtggaataa tattttcgac accatcaata atcaaaatat aagattgatt
     1681 ttctctagtt ttaaaatctt ctaatataga agacaccact tcaatcggat aatctgtttc
     1741 cttagcaata tactccatca aatccggaat ctcggattct ctttctaaac catgccaaaa
     1801 atattgttct ctgtcataat ttctaaaggc cgaatactta actaatggac aaccttgctt
     1861 taataattta agcggatgaa tatacaatgc actatctttc gtgtctttaa tcaatgcatc
     1921 atacttaaag cctcgatcat aaaaatattt tgcaaacaca gtttcatgtt ttccaattgc
     1981 cttattacga gaatccgtat cagagagctt ttcccaataa tcataaaatc cttcatacct
     2041 aagcaagcta ttttcaataa caacaaaata tgattgtaag tgtttaggaa ttttaccata
     2101 agggttataa ccagtaaagt ctagttgctc ctcacccatg gaaattcccc aaaagtctag
     2161 ctgatcggaa ttaaactcag agaaaacctc ttccaaatct ctaaaaggac caatattagt
     2221 atcattaacc aagatgagct gattatactg ttgtaacgcc tcttttccag tatgtattat
     2281 cccatgtcgg aaagccgcaa cgtcataccc ctcattttct ctttccaaaa ccttaccata
     2341 ttgtgctaaa cgatttatat cagcctgtgg aagtttacca tttacaacaa ttaaaacatc
     2401 tcgagaaatt ttagccaatg cctctaaaaa aattacttta tagtcctgta aagacgattg
     2461 atgttcataa attgtgtaaa tcaagtaacg atttttgata tctgaataat tatttctacg
     2521 ttgcttatca cgttgcaaag ctagtagagt ccgctttata tattttggaa ttctccagaa
     2581 aaactttgca atatttttgg taagctctaa tatataaaac aatagcttgt atttacctga
     2641 taa
//
LOCUS       DAZQCJ010000149         2611 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000149 DAZQCJ010000000
VERSION     DAZQCJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.151"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            229..>2611
                     /locus_tag="ACHU66_002037"
     CDS             229..>2611
                     /locus_tag="ACHU66_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HFR3390291.1"
                     /translation="MNKKIFLNSAIILLSVTCFTGSVDALDNSRVIAIPSEIVNSENA
                     YNITLKAKTDGVESDLGGSIVSENGVLSGSNGTKINLKITTNPGYRFVGYQSFLENGN
                     STDGLLPIENDSFEISDKTGNVTVIAIFESVPVDESIYFSDDFSVENINNYVISENLV
                     GKIGISDGKMNLHAPGISSIKPILTKPIEDNATSGYEIGFTLQQIGEVKPWNTFRIVF
                     KEDHDGFIYALEFSGQAAFLKKLSSIDAPLQSGEKYAETSHILGSEEHKVRLVVKGNS
                     VTVSDNGIPLLSYNSPNNWNDAQASIVFSPVSNRSVSLDDIIVRRTQTLRPFRVVSRL
                     DGQEVSGVLPGSIKGTTSQVFVGDSLNLEVIEKSGYQFIGFKDSAGNFLDLTTFVVPN
                     EESELVLYADFESVDVVIREPKTFYIDSLGGNDSNNGETENSAWKSFEQLRKKSFVAG
                     DRILIKRGSRFVGESAFLTFKGSGSQNTPILISSYGEGELPLLEGQGKIENVIKLYNQ
                     EFITIENLEITNLDPNFSTSFELNSNNNRTKSLRGLHVVAEDYGVVHDINIRNLYIHD
                     INGNLSSKWNGGIFFDVYGTTIPTKYDGILIENNYLERVDRSGIKLVGSTWANQNLKN
                     NKNVPLNWYPSTNVVVRGNRIEKAGGDSLTVRDTDGALIEYNISADARYQNTGYNAGI
                     WPFQASNTVIQYNESFRTHGVQDGQGLDLDHISNNSVMQYNYSHDNEGGFMLIMNWYE
                     QTSPTVRYNISQNDKDKIFELARGGAQGTAIYNNTIYSDSKLTGRAGVIDMPST"
BASE COUNT          905 a          339 c          516 g          851 t
ORIGIN      
        1 gagagacttt ttagattata ttagaaatca tgaatcagat tttcaaccat atcttgatgt
       61 ttaccgtatc attgaagttg ctaaaaatta tgctggattc aaattgtagt cctatggatt
      121 agattagaaa aatagccaat tcactattta tttttaagtg aattggcttg atgtctatta
      181 ttagaaggat aaataaaaat atttgatgtt attttttgag agggttacat gaataaaaaa
      241 atttttttaa actcagcgat tatattactc tctgttactt gttttactgg ttctgttgat
      301 gctttagata atagtagagt gatagcgata ccaagcgaaa tcgtaaacag cgaaaacgca
      361 tacaacatta ctttaaaagc gaaaactgat ggagttgaaa gtgatcttgg aggaagtatt
      421 gtttctgaaa atggtgtctt atcaggttct aatggcacta aaataaattt gaagataacc
      481 acaaatcctg gttatcgatt tgtaggttat caatcatttt tagaaaatgg aaatagtacg
      541 gatggattgc tacctatcga aaatgatagt tttgaaattt cagataaaac aggcaatgtg
      601 accgtaatag caatatttga gtcagttcca gttgatgaat caatatattt tagtgatgat
      661 ttttcagttg aaaatattaa taattatgtg atatctgaaa acctggtagg aaaaattgga
      721 attagtgatg ggaaaatgaa tcttcacgct ccaggtatct cttcgatcaa accaatttta
      781 acaaaaccaa tcgaagataa tgcaacttct ggatatgaaa ttgggtttac attacaacaa
      841 attggtgaag taaagccatg gaatactttt agaatagtat ttaaagaaga tcatgatggg
      901 ttcatttatg ctttagaatt ttcaggacaa gctgcttttt taaaaaaact ttctagtata
      961 gatgcaccac ttcaatctgg cgaaaaatat gctgaaacaa gtcatatttt aggaagtgaa
     1021 gagcataaag tacgacttgt agtcaaaggt aattcagtaa cagttagtga taatggaata
     1081 ccattattaa gttacaacag cccaaacaat tggaatgatg cccaagcatc tattgttttt
     1141 tcaccagttt caaatcggag tgtttctcta gatgatatta ttgttcgaag aactcaaacc
     1201 ttaagacctt tccgggtcgt ttctcgatta gatggtcagg aggtatctgg agttctgcct
     1261 ggttcaatta aaggaaccac aagtcaagtt tttgttggag attcacttaa tttagaggtc
     1321 attgaaaaat ctgggtacca atttattggt tttaaagata gtgctggtaa ttttcttgat
     1381 ttaactactt tcgttgttcc aaatgaggag tcagagttag tgctgtatgc cgattttgag
     1441 tcagtggatg tagtgattcg tgaaccaaag actttctaca ttgatagtct aggaggaaat
     1501 gattctaata acggggaaac cgaaaatagt gcatggaaat catttgaaca attaaggaaa
     1561 aagtcgtttg tagctggaga tagaatatta ataaaacgtg gaagtcggtt tgttggagaa
     1621 agtgcttttc ttacatttaa aggttcgggt agtcaaaata ctccaattct aatttcatcc
     1681 tatggtgaag gtgagttacc tttattagaa gggcaaggga aaattgaaaa tgtaattaag
     1741 ttgtataatc aagagtttat tacaattgaa aatcttgaga ttacaaattt ggatccaaat
     1801 ttttctacaa gttttgaatt gaattctaat aataatcgta caaaatcact tcgtggacta
     1861 catgtggtcg ctgaggatta tggggtcgtt catgatataa atattcgaaa tctatatata
     1921 catgacataa atggtaattt aagttcgaaa tggaatggag gaattttctt tgatgtttat
     1981 ggtacaacta tacctaccaa atacgatggt atattaatag aaaataatta tctagaaagg
     2041 gtcgataggt cgggaataaa attagttgga tcaacatggg caaaccaaaa tttgaaaaat
     2101 aataaaaatg ttccgttaaa ttggtatcca tctacaaatg tagttgttag aggaaatcgt
     2161 attgagaagg caggtggtga ttctctaact gttagagata ctgatggtgc attgattgaa
     2221 tacaatattt ctgctgatgc tagataccaa aacactgggt acaatgcagg tatatggcct
     2281 ttccaagctt cgaatacagt tattcaatac aatgaatcat ttagaacgca tggtgttcaa
     2341 gatggtcagg gtttagattt agatcatatt tctaataatt cagtgatgca atataactac
     2401 agtcatgata atgaaggtgg gtttatgtta atcatgaact ggtacgaaca aacatctcct
     2461 acagttcgtt ataatatcag ccagaatgat aaagacaaaa tttttgaatt ggctagagga
     2521 ggagcacaag gaactgcaat ttacaataat acaatctact ctgattcgaa attaactgga
     2581 cgagcaggtg taattgatat gccaagtacg t
//
LOCUS       DAZQCJ010000150         2582 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000150 DAZQCJ010000000
VERSION     DAZQCJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.152"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(36..668)
                     /locus_tag="ACHU66_002038"
     CDS             complement(36..668)
                     /locus_tag="ACHU66_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFR3390292.1"
                     /translation="MKEFKTIREDGIVEEEIKKSRFICFMKRVTSEEEARDFINKIKK
                     EHYKATHNCSAFVLGENMEIKRSSDDGEPSGTAGVPMLTVLENHELTNVATVVTRYFG
                     GIKLGAGGLIRAYAGVVANAVKEIGVVEVKEQEGISISMSYTQYQEFANWRAEQGLEE
                     FDAQFTTEVSTMIFVDKEFLEQTLASLTDFYHGKVSAEKANSRIVEVPVY"
     gene            725..2017
                     /locus_tag="ACHU66_002039"
     CDS             725..2017
                     /locus_tag="ACHU66_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR3390293.1"
                     /translation="MKELENYYGRLFTKYQLTAKEREIAEKVPSITKKNNCFRCGTTF
                     KEENKLPNDAYYCRACLLLGRVRSDEKLYHFPQKDFPITKCLKWKGQLTDWQQRISDG
                     LVANVENNRATLVHAVTGAGKTEMIYHTVASVIDKGGAVCLASPRIDVCIELYKRLQN
                     DFSVPISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLVLIDEVDAFPYADNPMLYQA
                     ADNAVKEAGVQVFLTATSTDELDKKVRTGKLSRLSLPRRFHGNPLVVPQKVWFSKFDD
                     TLKKNRLVPKLKKAIEEQRKSGFPLLIFVPEISKGQEFTKIMKKTFPEETIGFVSSQT
                     ENRLEIVEGFRKREITVLISTTILERGVTFPCVDVFVVQANHYLYTASSLVQIAGRVG
                     RSIERPTGLLQFYHEGSTGAIEKAIAEIKQMNKEAGYV"
     gene            2010..>2582
                     /locus_tag="ACHU66_002040"
     CDS             2010..>2582
                     /locus_tag="ACHU66_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFR3390294.1"
                     /translation="MSNCLLCGQAMKNKTRFSDLIFFSKEKSGICEECFSTFEEIAEQ
                     HCPHCYKNGESESCKDCQYWQNQGKPVVHTALYQYNQAMAHYFSRYKFQGDYVLRNIF
                     AKKLRIALSQFPDYTIVPIPISQKRLSERGFNQVEGLLDATNIPYQSLLGKYESQKQS
                     SKNRAERLEAKQMFYLLDEKEVPEKILLFDD"
BASE COUNT          849 a          507 c          498 g          728 t
ORIGIN      
        1 taagtaaaaa aaaactatct cgccgatagc tttttttaat agacaggtac ctcaacgatt
       61 cgagagttag ctttttccgc ggatactttt ccgtggtaaa agtcagtgag acttgctaag
      121 gtttgttcaa ggaattcttt atctacaaaa atcatggttg aaacttcagt agtaaactgg
      181 gcatcaaatt cttccagacc ttgctcagct cgccaattgg caaactcttg gtattgggta
      241 taggacatgc taatggaaat cccttcctgc tccttaacct ccacaacccc gatttccttg
      301 actgcattag ccacgactcc agcgtaagca cgaataagtc cgcctgcccc gagtttgata
      361 cctccaaaat agcgggtaac caccgttgcg acattggtca gttcgtgatt ttctaaaacc
      421 gtcagcattg gcacaccagc agtaccagac ggctccccat catctgagga acgcttgatt
      481 tccatatttt ctccaaggac aaaagcagag cagttatgag tagccttgta atgttctttc
      541 ttgattttgt tgataaagtc gcgagcttcc tcctcgctgg tcactcgttt cataaagcag
      601 ataaatcgtg actttttaat ttcttcctca acaataccat cttctctaat tgttttaaat
      661 tctttcataa ccaaattgta ctaatttttt taaaaactga caagaaattc cgaataaata
      721 agcgatgaaa gaattagaaa attattatgg aagattattt accaaatacc aattgacagc
      781 aaaagaaaga gaaatagcag aaaaagtgcc aagtattaca aaaaagaata actgctttcg
      841 ctgtggaaca acttttaaag aagaaaacaa attgccaaac gatgcttatt actgtcgagc
      901 ctgcttgctt ctaggcagag tacggtcaga cgaaaaactc tatcattttc ctcagaaaga
      961 ttttccaatc actaagtgtt taaagtggaa aggtcaacta actgattggc aacaaagaat
     1021 ttcagatgga ctagttgcaa acgtggaaaa taatcgtgcg acattggttc atgcagtaac
     1081 aggagcaggt aagacagaaa tgatctacca caccgttgcc tcagtgattg ataaaggcgg
     1141 agcggtttgc ctagccagtc ctcgaattga tgtttgtatc gaactctata aacgtctgca
     1201 aaatgacttt tcagttccaa ttagtttact acatggagag tctgaaccct atttccgaac
     1261 cccattagtt gtagcaacca cacatcagtt attaaaattt tatcaggcct ttgatttggt
     1321 tttgattgat gaagtagacg cctttcccta tgcagataat cccatgctct atcaagcagc
     1381 agacaatgcg gtcaaggaag ccggtgttca agtttttctg acagcgactt caacagatga
     1441 attggataaa aaagtcagaa caggtaaatt aagtcgtctt agtttgccaa ggcgctttca
     1501 tggcaaccca cttgttgtcc cgcaaaaagt ctggtttagt aaattcgatg ataccctaaa
     1561 gaaaaataga ctagtcccaa agttgaaaaa agcgattgaa gaacagagaa agtcgggctt
     1621 tcccttactc atttttgtcc cagaaatctc caaaggtcaa gaatttacca agataatgaa
     1681 aaaaacattc ccagaagaaa caattggctt tgtatccagt caaacagaaa atcgccttga
     1741 aatagttgaa gggtttcgca agagagaaat cacagtctta atctcgacta ctattcttga
     1801 acgtggggtg accttcccat gtgtagacgt ctttgttgtt caagctaatc attacctcta
     1861 cacagcgtca agtcttgttc agattgcagg ccgggtcgga aggagtatag aacgtccgac
     1921 tggtttactt cagttttatc atgagggaag tacaggagcc attgaaaagg caatcgctga
     1981 aattaaacag atgaacaagg aggctggtta tgtctaattg tctattgtgc ggtcaagcta
     2041 tgaaaaataa aacaagattt tcagatctca tcttctttag taaagaaaaa tcgggcatct
     2101 gtgaagaatg tttttcgact tttgaggaaa tagcagagca acactgtcca cattgttata
     2161 aaaatggaga atcggaaagt tgcaaggatt gtcagtactg gcagaatcaa gggaaaccag
     2221 ttgtccacac agctttgtac caatataatc aagcgatggc acattatttt agtcgctaca
     2281 aatttcaagg agactatgtt ttaagaaata tttttgctaa aaagcttaga atagcgttaa
     2341 gccaatttcc tgactacaca attgttccca taccaattag tcaaaaacga ctatcagaac
     2401 gcggttttaa tcaagtggaa ggacttttgg atgctacaaa tattccttat cagtcccttc
     2461 ttggaaagta tgaaagccaa aaacaatcat ctaaaaatcg tgcagagaga ctagaagcaa
     2521 aacagatgtt ctatctatta gatgaaaaag aagtgccaga aaaaatatta ttattcgacg
     2581 at
//
LOCUS       DAZQCJ010000151         2569 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000151 DAZQCJ010000000
VERSION     DAZQCJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.153"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(57..1661)
                     /locus_tag="ACHU66_002041"
     CDS             complement(57..1661)
                     /locus_tag="ACHU66_002041"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFR3390295.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITDALKDKIKRAARTTDADVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSDNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEQPAG
                     QGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNMQAQNMDQIVCDHLKLDVPPADMTE
                     WTAMVNKVLNLQKKVKIALVGKYVELQDAYISVVEALKHSGYANDAAIELDWVNANDL
                     TAENVAERLGQAQGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCVEFA
                     RHVLGLEGANSFELDPETKYPIIDIMRDQIGVEDLGGTLRLGLYPSKLKRGSKAAAAY
                     DNQEVVQRRHRHRYEFNNDFREQFEKAGFVFSGVSPDNRLVEIVEIPENKFFVACQYH
                     PELQSRPNRPEGLYTAFVSAAIENEK"
     gene            complement(1854..2435)
                     /gene="rpoE"
                     /locus_tag="ACHU66_002042"
     CDS             complement(1854..2435)
                     /gene="rpoE"
                     /locus_tag="ACHU66_002042"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFR3390296.1"
                     /translation="MELNVFAGQEKSELSMIEVARAILEERGRDNEMYFNDLVNEIQN
                     YLEKSNSEIRAALPTFYSDLNVDGSFIPLGENKWGLRSWYAIDEIDEEVITLEEDDED
                     APKRKKKRVNAFMDGDDDAIDYGHDDPEDEDNYPGSVSSEYDDENPDDEKDEVESYDS
                     EINEIIPDDELDEEDVDLGEDDDEYSDEEVVDE"
BASE COUNT          691 a          572 c          471 g          835 t
ORIGIN      
        1 tataaaatat aaaagaagat tgaataatac caatctcctt ctataaattg ttatacttat
       61 ttttcattct ctatagctgc tgaaacaaag gctgtataca aaccttcagg gcgattagga
      121 cgagattgta gttctggatg gtattggcag gcaacgaaga atttattttc tggaatttca
      181 acaatttcta ccaaacggtt gtctggtgac acacctgaga aaacaaaacc tgctttttca
      241 aattgttcac ggaagtcatt attaaactca taacgatgac gatggcgacg ttgtacaact
      301 tcttgattat cataagcagc tgcagccttg gagccacgtt tgagcttgct tggataaaga
      361 cctaaacgga gagttcctcc taaatcttcc actccaattt gatcacgcat gatgtcaata
      421 attggatatt tggtttctgg gtctaattca aagctgttag caccttctaa accgagaacg
      481 tgacgagcaa attctacaca ggtcagctgc attcccaagc agacccctag cattggcacg
      541 tcattctcac gagcataacg aatggcttgt attttccctt ctgtaccacg ttgtccaaag
      601 ccaccaggaa cgataattcc ttgtgcttgt cctagacgtt cagctacgtt ttctgctgtc
      661 aaatcatttg cattgaccca gtccaattcg atagcagcat catttgcata tcctgagtgt
      721 tttagagctt caactacaga aatataggca tcttgtaatt ctacatactt accaactaaa
      781 gcgattttta cttttttctg gagattaaga accttgttta ccatcgctgt ccactctgtc
      841 atatctgctg gaggtacgtc gagcttcaag tgatcgcaaa caatttgatc catgttctgc
      901 gcttgcatat ttaatgggat ttggtaaaga tgttcgacat ccaaggactc aataactgct
      961 tctggtgcaa catcacagaa ttgtgccagt ttatttttaa ttccttgtcc agcaggctgc
     1021 tctgtccgaa ttaccaacat gttgggctga atacctagac cacgcaactc ttttacagaa
     1081 tgttgtgttg gcttagtttt catttcgcct gctgctttga gataaggcaa gagtgttgta
     1141 tggatataca taacattgtc tgaaccgaca tctgccttca tctgacgaag cgcttcaagg
     1201 aatggcaagc tttcaatatc acctactgta ccacccactt ccgtaatgat gacatctgca
     1261 tctgtagtac gagctgcacg cttgattttg tctttcaaag catctgtaat gtgtggaata
     1321 acttgaactg ttgcccctaa gtattcccct ttacgctctt tacggaggac ttcgctataa
     1381 attttacctg ttgtaacatt tgaatatttg ttcaggttaa tgtcgatgaa acgctcataa
     1441 tgcccaaggt ccaagtcggt ttctgcgccg tcgtccgtca caaaaacttc accgtgttga
     1501 tacggactca ttgttcccgg gtcaatattg atgtatggat caaatttttg gattgttacc
     1561 ttgagacctc ggttcttcaa taaacgaccc aggctggctg ctacaatccc tttgccgata
     1621 gaggaaacaa cgccacctgt tacaaaaata tactttgtca tgcaaactcc ttttctacga
     1681 ttcaggctaa taggaagcaa tttgtgggga tacttcctgt taacttgaag aaaaaacaaa
     1741 aatagctccc tatcgctagg gagctccgac ctcaaaagag gtgcccgata atatcctatc
     1801 gtaattcagt agtcttgtca aattattttt aagaatctat ggggatataa ttcttattcg
     1861 tcgacgacct cttcatctga atactcgtcg tcatcttcac ctaaatcaac atcctcttca
     1921 tccaattcgt catctgggat aatttcgttg atttctgaat cataggactc aacttcatct
     1981 ttttcgtcat ctggattttc atcatcgtat tctgatgata cactacccgg atagttatcc
     2041 tcatcttccg gatcatcatg tccatagtca atcgcatcat catcaccgtc catgaaggca
     2101 ttgacacgtt tcttcttgcg ttttggtgca tcttcgtcat cttcttcaag agtgatcact
     2161 tcttcgtcga tctcatcgat tgcgtaccaa gaacgtaagc cccatttgtt ttcgccaagt
     2221 ggaatgaagc ttccatccac attcaaatca gaatagaagg ttggaagggc tgcgcgaatc
     2281 tcgctgtttg atttttcaag gtagttttga atttcattga ccaagtcgtt gaaatacatt
     2341 tcgttgtcac gtccacgttc ttctaaaata gcacgggcta cttcaatcat ggaaagttcg
     2401 cttttttctt gtcctgcaaa tacgttaagt tccaaggctg ttctcctttt ttgtctttcc
     2461 tttctatttt acgctaaaaa aggcaaatag tcaaagataa tctttacccg tggtgctagt
     2521 tcatttcaaa atagcgtaag ttatgagcta gaataatttg ttccattcg
//
LOCUS       DAZQCJ010000152         2527 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000152 DAZQCJ010000000
VERSION     DAZQCJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.154"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..532
                     /locus_tag="ACHU66_002043"
     CDS             <1..532
                     /locus_tag="ACHU66_002043"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR3390297.1"
                     /translation="CDEYIHENKLQKNVKFLGNLDNPWEEVTDRDICVFPSSMETFGL
                     VYVEAILRGLPVILSDNLGHLTAFEIFSAGQIYKLGDIEELVTKLEQVIESFRNFSDR
                     ARLDVERLRTLYTPEVAYKEILNSINHLEYTGHQNTNLSHIKELLTLNMHPTRLERYA
                     RLISNMATKIRHKLRL"
     gene            636..1352
                     /locus_tag="ACHU66_002044"
     CDS             636..1352
                     /locus_tag="ACHU66_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR3390298.1"
                     /translation="MKVLMIIPAYNEEESILQTVQGIIDYRNSVNFQLDYVVINDGST
                     DSTKEILIQNKLNAVHLVQNLGIGGAVQTGYKYALDNDYDVAVQFDGDGQHDVRSLNS
                     LIQPILVGQADMVIGSRFVGETLSEFQTSFMRRFGIGVISNMIKLTTGNRIWDTTSGY
                     RLGNRKVIAQFAKRYPIKYPEPESTVHLLKQNFQVVEAPANMFERAGGVSSITPFKSI
                     RYMVEVCSSILIASLMKEGE"
     gene            1352..1708
                     /locus_tag="ACHU66_002045"
     CDS             1352..1708
                     /locus_tag="ACHU66_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HFR3390299.1"
                     /translation="MTIQALAMFLASLGFLYFIFRNINKNKILFEHAFMWIVIGFGLI
                     VFALFDVIPIKLAYLFGFGLTSNFLLSVAIFVLLVIGFLHSMALSQQKQQIKNLIQEV
                     SMAKKRISELEEHHAE"
     gene            1698..2468
                     /locus_tag="ACHU66_002046"
     CDS             1698..2468
                     /locus_tag="ACHU66_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR3390300.1"
                     /translation="MQNNHTFVICAYGDSKYLEECIHSLKQQTLQSQLILYTSTPSEY
                     IEQICQKNAVVMHTAQGGGIGKDWNLALSFVTTPYATIAHQDDIYLPDYCEKIMSQMT
                     EETLIAYSDYQEVKEGVAISLTSNLKIKQLMLRTMAMFPKWKFWRNRVLAFGNPISCP
                     AVTYNLKKLNDFKFNEEMKVSLDWFAWYQIAQKNGSFTFVDESLMYHRIHEESETTNS
                     IENNIRTKEDYEMYLLFWPKFIAKFLLSYYVKSQETNN"
BASE COUNT          840 a          374 c          493 g          820 t
ORIGIN      
        1 ttgtgatgaa tatatccatg aaaataaatt acagaaaaat gttaaatttt taggtaactt
       61 agataatcct tgggaagaag tgactgatag agatatctgt gttttcccct catctatgga
      121 aacttttggc ttagtctatg ttgaagcaat cttaagaggg cttccagtaa ttctatcaga
      181 taatttagga catttgacgg catttgaaat attctcagca ggtcaaattt ataaattggg
      241 cgatatagaa gagttggtga ctaagttaga gcaagtgatt gaatccttta gaaatttttc
      301 agatagggct cggcttgatg tggaacgttt acgtactcta tatacaccgg aagttgccta
      361 taaagaaata ctgaacagca tcaatcactt agaatatact ggacaccaaa atacaaattt
      421 aagtcacata aaagagttac tgactttaaa tatgcatcca acaagattgg agcgttatgc
      481 aagattgatc agcaatatgg cgacaaaaat cagacataaa cttcgacttt aatgaagaat
      541 aggatagcta tgctaaaaga tgtatattat agtttcttca aatgagtaag tagaatctaa
      601 aaagttatat agaattctag ttttagagag aaaaaatgaa agtattaatg attatccctg
      661 cctacaatga agaagaaagt atccttcaaa ctgttcaggg aattatagat tatagaaata
      721 gtgtgaattt ccaattggat tatgttgtca ttaatgatgg ttcaacagat agcacgaaag
      781 aaattttgat tcaaaataaa ttaaatgctg ttcacctagt acaaaattta ggcattggtg
      841 gagctgttca gacgggatat aaatatgcct tggacaatga ctatgatgta gcagttcaat
      901 ttgatggtga tggtcagcat gacgttcgtt cattaaatag cttaattcag ccaatcttag
      961 ttggtcaggc tgatatggtg attggctcac gttttgttgg tgagaccttg tcagaatttc
     1021 agactagttt tatgagacga tttgggatag gtgttatttc aaatatgatt aaattaacaa
     1081 ctggaaaccg aatttgggat actacatcag gttatcgttt gggaaataga aaagtgattg
     1141 cacaatttgc aaaacgatac ccaattaaat atccggaacc ggaatcaact gttcatttac
     1201 taaagcaaaa tttccaagta gtagaagcac cggccaatat gtttgaaaga gcgggagggg
     1261 tttcttcgat tactccattt aagtccatcc gctacatggt agaagtttgc tcatctattt
     1321 tgattgcttc gcttatgaag gagggagaat aatgaccata caagcattag ctatgtttct
     1381 tgcttcacta ggttttttgt attttatttt taggaatatt aataaaaata agatcttatt
     1441 cgaacatgcc tttatgtgga ttgttattgg cttcggactg attgtttttg cgctctttga
     1501 tgttattcct attaaattgg cttacttatt cgggtttggt ctgacttcca atttcttgtt
     1561 atctgtggca atttttgttc tcttggtgat tggatttttg catagtatgg ctttatctca
     1621 acaaaagcag cagattaaaa atttaattca agaagtatca atggctaaaa aaagaatctc
     1681 tgagttggag gagcatcatg cagaataacc atacatttgt tatctgtgct tatggagata
     1741 gtaagtatct agaagaatgc atccattcgt taaagcagca aacacttcaa tcacaactca
     1801 tcctatacac tagtactccg agtgaatata tagaacaaat ttgccaaaag aatgcagtcg
     1861 taatgcatac cgcacaagga ggaggtattg gaaaagattg gaatttagca ctctcttttg
     1921 taacaacccc ttatgctaca attgctcatc aagatgatat ttatttacca gactattgtg
     1981 aaaaaataat gtctcagatg acagaggaaa cgttgattgc ttattcagat taccaggaag
     2041 ttaaggaagg tgtggctatt tccttaactt caaatttgaa aatcaagcaa ctcatgttac
     2101 ggacaatggc catgtttccc aaatggaagt tttggcggaa tagggtttta gcctttggta
     2161 atccaatttc ttgtccagct gtgacctata atttgaagaa actgaatgat ttcaagttta
     2221 atgaagaaat gaaagtttct cttgattggt ttgcttggta tcagattgca caaaaaaatg
     2281 ggagctttac ctttgtagat gagagtttga tgtatcatcg tatccatgaa gaatcggaga
     2341 cgacaaatag cattgaaaac aatattcgta caaaagaaga ttatgaaatg tatttgttat
     2401 tctggccaaa gtttattgcc aagtttctct tgagttacta tgttaagagt caagagacga
     2461 ataactgaaa tattttagct atcgatcatt gcgttcccac caattcctac tcaaaatatt
     2521 tcaaaag
//
LOCUS       DAZQCJ010000153         2467 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000153 DAZQCJ010000000
VERSION     DAZQCJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.155"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            4..645
                     /locus_tag="ACHU66_002047"
     CDS             4..645
                     /locus_tag="ACHU66_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR3390301.1"
                     /translation="MDYVLEVLPSLLNGAAVSLQVFFLVLILSLPLGAVFAFLMQIKF
                     KPLQWLLHFYVLIMRGTPLLLQLIFVYYVLPSVGIIFDRMPAVILAFTLNYAAYFSEI
                     FRGGIEAIPKGQYEAAKVLKFTPVQTIRYIVLPQVVKIVLPSVFNEVMTLVKDTSLVY
                     ALGVSDLLLASRTAANRDASLAPMFIAGAIYLLMIGAVTLISKQVEKKFDYYR"
     gene            655..1287
                     /locus_tag="ACHU66_002048"
     CDS             655..1287
                     /locus_tag="ACHU66_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR3390302.1"
                     /translation="MLELRNLSKRFEDKQIFSNYDLVIPEGKIVAIVGQSGGGKTTLL
                     RMLAGLETIDSGTLMYNGQELPLEELGKRHLLGFVFQDFQLFPHLSVLENLVLSPMKT
                     QNMSRSEAEDKALKLLDTLGLANHATAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRKEVEKLILENRATGITQIVVTHDMQFAENIADEIIKIEPKH"
     gene            1314..2144
                     /locus_tag="ACHU66_002049"
     CDS             1314..2144
                     /locus_tag="ACHU66_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFR3390303.1"
                     /translation="MKKMMLGALALVTSLTLAACGSNDSATKSDNWTAYESEKSVTIG
                     FDKTFVPMGFEQTDGSYTGFDIDLANAVFEKYGITVKWQPIDWDLKETELNNGNIDLI
                     WNGYSITDERKEKVLFTNPYMDNQQVLVTKKSSNISQVSDMKDKILGAQAGSSGYSVF
                     ESQPAILKDIVQNNDASQYATFNEALIDLKNDRIDGLLIDRVYANYYLQQEGIIADYN
                     IIDAGFENEAFAVGARKSDTTLVENINKAFAELYKEGKFQEISQKWFGEDVATDAVKK
                     "
BASE COUNT          732 a          439 c          541 g          755 t
ORIGIN      
        1 tctatggatt atgttttgga agttctgccc agtctactaa atggtgctgc agtctccttg
       61 caagtatttt ttctagtatt gatattatcc ttgccgttag gagctgtttt cgctttttta
      121 atgcagatta aatttaaacc cttacaatgg ttattgcatt tctatgtatt aattatgcga
      181 ggaaccccct tgcttttaca attaattttt gtttattacg tcttaccaag tgtgggtatt
      241 atatttgatc gaatgcctgc tgtgattctg gcctttacgc tcaactatgc ggcttatttc
      301 tctgaaattt tccgtggtgg tattgaagct attccaaaag gtcagtacga agctgctaag
      361 gtattgaaat ttacccctgt tcagaccatt cgctacattg tcttaccaca ggttgtgaaa
      421 attgttctgc caagtgtttt caatgaagtc atgactttgg taaaggatac ttctttggtc
      481 tatgcacttg gggttagtga tttgttgttg gctagtcgta ctgcggccaa tcgtgatgcc
      541 agcttggcgc ctatgtttat cgcaggtgca atctacctat tgatgatcgg tgcggtaaca
      601 ctgatttcta aacaagtaga aaagaaattt gattattata gataggagga agccatgtta
      661 gagttacgca atttgtcaaa acgctttgaa gataaacaga ttttctccaa ctatgatttg
      721 gtcattccag aaggaaaaat tgttgccatc gttggtcaat ctggcggtgg taagacaact
      781 cttttacgga tgttggctgg tttagagact attgattcag gaactttgat gtataatggt
      841 caagaactgc cattagagga gttgggaaaa cgacatttgc tgggctttgt cttccaggac
      901 ttccagcttt tcccgcacct atccgtattg gaaaacttgg ttctctctcc tatgaaaacg
      961 caaaatatgt cgcgttcaga ggcggaagac aaggccctta aactcttgga tactcttgga
     1021 cttgctaacc acgcaactgc ctacccattt tcattatcgg gtggacagaa acaacgtgtg
     1081 gccttggcgc gtgcgatgat gattgatcct gagattatcg gctatgatga accaacttcg
     1141 gccctagacc ctgaattaag aaaagaagtt gagaaactga ttcttgaaaa tagggctact
     1201 ggaatcacgc aaattgtggt cacccatgat atgcagtttg ctgagaatat cgcagatgaa
     1261 atcatcaaaa ttgaaccgaa acactaagaa aaggaacaaa tatgatgatg aatattaaaa
     1321 aaatgatgct tggagctctt gctcttgtaa cttctttgac tcttgctgct tgtggctcca
     1381 atgattcggc tactaagtct gataattgga cagcctacga gtctgaaaaa tccgttacga
     1441 ttggctttga taagaccttt gtcccaatgg gatttgaaca gacagatggt tcttacacag
     1501 gtttcgatat tgacttggct aatgcagtct ttgaaaaata tggaattact gtaaaatggc
     1561 aaccaattga ctgggacttg aaagaaactg aattgaataa cgggaacatt gatttaattt
     1621 ggaatggtta ttctattaca gatgagcgta aggaaaaagt tcttttcaca aatccctaca
     1681 tggataacca acaagttctt gtaactaaaa aatcttccaa tattagccaa gtatctgata
     1741 tgaaggataa gattttaggt gcgcaagcag gttcatctgg ttattcagta tttgaatcac
     1801 agccagctat tttgaaagat attgttcaaa ataacgatgc aagtcagtat gcgaccttca
     1861 atgaagcctt gattgacttg aaaaacgatc gtattgatgg ccttttgatt gaccgtgtgt
     1921 atgcaaatta ttacttacaa caagagggaa ttatcgcaga ttacaacatc attgatgcag
     1981 gttttgaaaa tgaagccttc gcagtcggtg ctcgtaaatc agatacgaca ctcgtagaaa
     2041 acattaataa ggcgtttgct gaattatata aagaaggtaa attccaagaa atctcccaaa
     2101 aatggtttgg tgaggatgtt gcaaccgatg cagtaaaaaa ataataatgt aagaaaaagt
     2161 gactgaacaa gacatccatg atggttacgc tctcatccac agaataatga aaagaactcg
     2221 tgaaaacggt gggggtgtac accaatatca ttaacttaag attctacgag gctgagcaaa
     2281 aagctcagcg ccacttctcg gagttcgtgt caacatctca gcgcagtggt tgattggctc
     2341 caacagtcac tattctgact gttggagcta tgcgggggtg ggagtttaaa aggtctggga
     2401 gaccttttaa agtgggagat ataagctgac ggcagtcagc tcacataaat tgatgtctgg
     2461 ggataga
//
LOCUS       DAZQCJ010000154         2444 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000154 DAZQCJ010000000
VERSION     DAZQCJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.156"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(92..637)
                     /locus_tag="ACHU66_002050"
     CDS             complement(92..637)
                     /locus_tag="ACHU66_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390304.1"
                     /translation="MSLQNRVEEMYKDHEVKPYISPERDLAAWLLEAKPVPKRNMVRL
                     EEGILPGDIILLWRISLGSFETTTPYSKYFEYMYGINGPAHMEQLIADGYAYVESAFD
                     SLDHITSTAKKNILKAEGVTGLSKMKAADLDTALKDNLTEEKLAPYFTVRGYALTEKG
                     RAALENHPEVLAKHPMKKMYK"
     gene            complement(638..1015)
                     /locus_tag="ACHU66_002051"
     CDS             complement(638..1015)
                     /locus_tag="ACHU66_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HFR3390305.1"
                     /translation="MIQSIGQVMLYVNDIEASAQFWKEKMGFERVEKQVQGPQTSYII
                     APKSDSEVQFVLHDKAEVAEMEPELNLGTPSILMTSVDVAKTYEELVERGVTTNPVMD
                     LGFMKVISFADNDGNYYAIREVK"
     gene            complement(1100..1453)
                     /locus_tag="ACHU66_002052"
     CDS             complement(1100..1453)
                     /locus_tag="ACHU66_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR3390306.1"
                     /translation="MFENLFAPDGSLVWHTALFYSFSAIILATVCFHIIKNFMEWHHN
                     NRAPKESCKAKLVSKRTHVFGNEVARTNYYVTFEWEGKRQEFRLRSDEYALLAEGDVG
                     TLHFQGTRFLGFERL"
     gene            1596..2399
                     /locus_tag="ACHU66_002053"
     CDS             1596..2399
                     /locus_tag="ACHU66_002053"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR3390307.1"
                     /translation="MKKILVLALASSILLTACTNNSTANSSSSTASSSSPSSTTTADT
                     TASSQYAKDLAYAINNPKATFPQLSNEIGENEAAVKIKTTEGDITIKLFPEQAPLTVE
                     NFLTHAKEGYYNGTIFHRVIKDFMIQGGDPLGNGTGGESIWSGKDTTIDAGNGFKDEI
                     SAFLYNIRSSLSMANAGAGTNGSQFFINQNTTDMSSQLSSSSYPGKIIDAYKNGGNPN
                     LDGKHTVFGQVIEGMDIVDKIASVETDNSDKPKTDVKIESIEILKDYTF"
BASE COUNT          736 a          527 c          465 g          716 t
ORIGIN      
        1 gtcaagggaa atagacttat tagcaaataa aaacacccaa aaagctagaa tgattccaac
       61 ttttgggtgc cgttcaaaaa tcttttctat tttatttgta catcttcttc attgggtgtt
      121 ttgcaagtac ttcagggtga ttctcgagag cagctctgcc tttttccgtc aaggcataac
      181 ctcggacggt aaagtatgga gccagttttt cttctgtcaa gttgtccttg agggcagtgt
      241 ccaggtctgc cgccttcatc ttggacaggc ctgtcacacc ctctgctttg aggatatttt
      301 tcttggcagt cgaagtgatg tggtccagcg aatcaaaggc cgattccaca taggcataac
      361 cgtctgcgat caactgctcc atgtgagcag gaccattgat accatacata tactcaaagt
      421 acttggagta gggcgtcgtc gtttcaaaag aacccaggct aatccgccat aataaaataa
      481 tatcacctgg caaaattcct tcttctagcc gaaccatatt gcgtttggga acgggcttgg
      541 cctccaaaag ccaggctgcc aagtcacgtt cgggggaaat atagggtttg acctcatggt
      601 ctttatacat ttcttcaaca cgattctgga gtgacattta ctttacctca cgaattgcgt
      661 aataatttcc atcattgtct gcaaagctta ttaccttcat gaaaccaagg tccatgactg
      721 gattcgttgt gacaccacgt tcaacaagtt cttcgtaagt tttggctaca tctactgatg
      781 tcattaagat tgatggtgta cctaagttta attctggctc catctcagca acctccgcct
      841 tatcatgaag tacaaattgt acctcactat ctgatttagg tgcaataatg taagaagttt
      901 gggggccttg cacttgtttt tctacacgtt caaatcccat tttctctttc caaaattgag
      961 ctgaagcctc aatatcattc acatataaca taacttgtcc gattgattga atcatcttaa
     1021 atacctcttt tgcgttgtac gaacatgtgt tcaaaccatt atacactaga tagggatatt
     1081 ctccaaagaa aaacgactct tagagccgtt caaaacctag gaaacgagtc ccctgaaaat
     1141 gcagggtgcc tacatctccc tctgctaaaa gggcatattc atctgaacga aggcgaaatt
     1201 cctgccgttt tccctcccat tcaaacgtta cataataatt ggttcgtgct acttcgttac
     1261 caaaaacatg ggttcgttta gagacaagct tagccttgca agattctttg ggcgcccgat
     1321 tgttgtggtg ccattccata aaatttttaa tgatatggaa acaaaccgtc gctaatatga
     1381 ttgcggaaaa actgtagaaa agggccgtat gccatactag gcttccatct ggggcaaaca
     1441 agttctcaaa catcattatt ctccttttgc atctgtaaat atagtatatc atattgttgt
     1501 tttagatacg ctttttatac tagataaaaa ttgaaaaaat ccatatttca tggtagaatg
     1561 agcataattt tgaacaagac aagaaaggat tttcaatgaa aaaaatacta gttcttgctc
     1621 tagcttcgag catcttgcta actgcctgca caaacaactc gacagctaat agctcatctt
     1681 ctacagcatc tagctctagt ccaagctcga ctacgacagc tgatacaact gcttctagcc
     1741 aatatgccaa agatttggca tacgccatta acaatcccaa agctacattc ccccaacttt
     1801 ccaatgaaat tggtgaaaat gaggctgctg ttaaaataaa aacaaccgaa ggcgacatca
     1861 ctataaaact cttccccgag caagctccac taactgtgga aaactttctg actcatgcga
     1921 aggaaggata ctataatgga acaatcttcc atcgtgtcat taaggatttc atgattcaag
     1981 gtggcgatcc tcttggaaat ggtactggtg gagagtctat ctggtccgga aaagatacta
     2041 ctatcgatgc tgggaatggt tttaaagacg agatttcagc tttcctttat aacattcgta
     2101 gctcactttc tatggcaaac gctggcgcgg gaacaaatgg aagtcagttt ttcatcaatc
     2161 aaaatacaac agacatgtct agccaactct ctagttctag ctatcctggt aaaatcattg
     2221 acgcttataa aaacggaggc aatccaaact tagatggcaa gcataccgta tttggtcaag
     2281 tcattgaagg gatggacatc gtggataaaa tcgcttcagt cgaaacagac aatagcgata
     2341 aacctaaaac agatgtaaaa attgagtcta ttgaaattct gaaagattac accttttaaa
     2401 aatgggttct ataatttctg tagtgggtaa aaccaccgtg gaga
//
LOCUS       DAZQCJ010000155         2356 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000155 DAZQCJ010000000
VERSION     DAZQCJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.157"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..959)
                     /gene="csn2-St"
                     /locus_tag="ACHU66_002054"
     CDS             complement(<1..959)
                     /gene="csn2-St"
                     /locus_tag="ACHU66_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csn2-St"
                     /protein_id="HFR3390308.1"
                     /translation="MKWLITHHVLNNIPIHVGQFTQIVGQNYELKYYIWQILIWYFGG
                     KKYSEEDLILFNQEEPVISQDSEAIKRNAFKIISIAEVGDILEQISYKKGTIGFSYLS
                     LKMQNIEIIEELEILNYHLHKIAQKVNSSISLINDEIEYEVGTTDLLPEQILTKQLTP
                     HFKKSRDEIAIEFISNEKKLCFLLQMLNAIMQEQTKPILLVLKNLDDYLTYDSFVRIA
                     QYLEELSNKYPYFNTILFPSQEGYLYLTEATLETVNIVSDRIEHYPAFTFLYTRYQQS
                     YPSTSPLGEKEFLNSLRKISSYLFSSDINRVVSLADIDLVTLK"
     gene            complement(956..1279)
                     /gene="cas2"
                     /locus_tag="ACHU66_002055"
     CDS             complement(956..1279)
                     /gene="cas2"
                     /locus_tag="ACHU66_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HFR3390309.1"
                     /translation="MRYDAMRLLCFFDLPMETTQEKRQYRLFRKELIANGFEMLQFSV
                     YYRTCPNRSFATKFYKKLQQSHLPAGNVRLLAVTEKQFSEMVLIVGGKTRQEEAISDR
                     KLVII"
     gene            complement(1276..2190)
                     /gene="cas1"
                     /locus_tag="ACHU66_002056"
     CDS             complement(1276..2190)
                     /gene="cas1"
                     /locus_tag="ACHU66_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HFR3390310.1"
                     /translation="MTWRIVHIHQSEKMRLKLDNLVIKKQGDEFTIPLSDISIIVAEG
                     GDTVVTLRLLSALSKYNIALIVCDNHHLPTGIYHSQNGHFRAYKKLQAQLSWAPLQKD
                     KLWQIIIYFKITNQQDVLAMQEKSLSTIQLLSDYREHIELGDRTNREGHAAKVYFNEL
                     FGKQFVRLTQQETDAVNAGLNYGYTIFRAQMARIIAGYGLNALIGIFHKNEYNQFNLA
                     DDLMEPFRQIVDVWVYMNLREADFLTYQHRLELTNLLNAKIRYGKENCSVTVAMDKFV
                     KGFIRCIEDKDTNQFFCPVVSSLEMEKL"
BASE COUNT          726 a          464 c          353 g          813 t
ORIGIN      
        1 atctttagag ttactaggtc tatgtctgct aagctcacga ctctattaat gtctgaagag
       61 aataaatagg aactgatttt tctcaaagag tttaaaaatt ctttttcacc tagtgggcta
      121 gttgagggat atgactgctg ataacgagta tagagaaacg tgaaagcagg gtagtgttca
      181 attctatcag acacaatatt aacagtctca agcgttgcct cggtcaagta caaatagccc
      241 tcttgagaag gaaacaagat tgtgttgaaa tagggatact tattactaag ttcctctaaa
      301 tactgtgcta ttctcacaaa agaatcgtaa gttaggtaat catctaaatt tttaagtacc
      361 agtaaaattg gttttgtttg ttcttgcatt attgcattca acatttgcaa tagaaaacac
      421 aatttctttt catttgaaat aaattcaata gcgatctcat ctcttgactt tttgaaatgc
      481 ggagtaagtt gctttgttaa aatttgctct ggaagtaaat cggtcgttcc aacctcatac
      541 tcgatttcat catttatcaa cgaaatgctt gaatttactt tttgagcaat cttatgtaaa
      601 tgataattta atatttcaag ctcttcaata atctctatgt tctgcatttt taatgacaaa
      661 tatgaaaatc caatcgttcc ctttttatag ctaatctgct ctaggatatc tccaacctct
      721 gctattgaaa tgattttaaa agcatttctt tttatcgctt cactgtcctg tgaaatcaca
      781 ggctcctctt gattaaaaag tattaaatct tcctcactgt acttttttcc accaaaatac
      841 catatcaata tttgccaaat ataatatttt aattcgtagt tttgaccgac aatttgagta
      901 aattgaccaa catgaattgg aatattattt aatacatggt gtgttattag ccacttcata
      961 tgattaccaa cttcctatca cttatcgctt cttcctgtct tgtcttacca cctacaatta
     1021 ggaccatctc agaaaattgt ttctccgtca ccgctaataa tctcacatta ccagctggta
     1081 gatgactttg ttgtaacttt ttataaaact tcgttgcaaa actccgattc ggacacgtac
     1141 gataatagac agaaaactgt aacatttcaa agccattagc aatcaactct tttcgaaaca
     1201 agcgatactg acgtttctct tgagtcgttt ccattggcaa gtcaaaaaag cataacaggc
     1261 gcatagcatc atacctcata atttctccat ttctagactt gaaactactg gacagaaaaa
     1321 ttgatttgtg tctttatctt cgatacaacg aataaaccct ttgacaaact tatccattgc
     1381 gacggtgaca ctgcaatttt cttttccata tctaatcttg gcattcaata gatttgttaa
     1441 ttccaaacga tgttggtatg ttaaaaagtc agcttccctc aaattcatat agacccatac
     1501 atcaacaatt tgtcgaaacg gctccattaa atcatctgct aaattgaatt ggttgtattc
     1561 atttttatga aaaattccta ttaaagcgtt taaaccatat cctgctataa ttcgtgccat
     1621 ttgagctcta aaaattgtgt agccataatt caaccccgca ttcactgcat cagtctcttg
     1681 ttgtgttagt cgaacaaact gtttgccaaa cagctcattg aaatacacct tagcggcatg
     1741 cccctcccta ttcgtcctat ccccaagttc gatatgttcc ctataatcag ataataactg
     1801 aatggtagac aaagattttt cctgcattgc caaaacatct tgctggttcg taattttaaa
     1861 atatatgata atttgccata atttatcctt ttgtagcggc gcccatgata gctgagcttg
     1921 caacttttta taagctctaa aatgtccatt ttgagagtga taaatacctg taggtaagtg
     1981 gtggttatca cagactatta aagcaatatt gtacttacta agtgcactta ataggcgaag
     2041 agtaacaact gtatcccctc cttctgcaac aattatagaa atatcactta atggaatcgt
     2101 aaactcatcc ccttgtttct ttatgactaa gttgtcgagc tttaatcgca ttttttcact
     2161 ttgatgaata tgtacaattc tccaagtcat ggtgttctcc tgtttataac gaaaagcacc
     2221 ccgtacgaaa cggggtgctt gatttcggca taaagcctta tctttgtagg ctctgcaaga
     2281 tttaagtaac agtgtaaggc ttcccttaca gttaaagttt acacttttta cttaaaaaat
     2341 acaacccctt ttttaa
//
LOCUS       DAZQCJ010000156         2353 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000156 DAZQCJ010000000
VERSION     DAZQCJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.158"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(48..2066)
                     /gene="recG"
                     /locus_tag="ACHU66_002057"
     CDS             complement(48..2066)
                     /gene="recG"
                     /locus_tag="ACHU66_002057"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR3390311.1"
                     /translation="MKRLCDELSVLPGIGPKSAEKFLKINIQNINDLLTYYPFRYEDF
                     ESKSIYDLQDGEKAVVVGEVVSPANVQYYGYKRNRLRFSMKQGEVVLAVSFFNQPYLA
                     DKIVLGQDIAVWGKWDKAKASLTGMKILAQVSDELQPVYHVAQGISQVNLVKAIKTAI
                     DQGYLHLLEENLPSVLRERYRLMNRREAVFAMHFPTNLEEYRQALRRMKFEELFYFQL
                     QLQMLKANNRDISNGLKIAYDADRLAMQIRQLPFVLTDAQSGALAEILSDMKSYGHMN
                     RLLQGDVGSGKTVVAGLAMFAAVTAGMQAAIMVPTEILAEQHFESLRQLFPELSIALL
                     TGGMKAAERRTALEAISSGQVDIIVGTHALIQESVTYHKLGLVVTDEQHRFGVKQRRL
                     FREKGDNPDVLMMTATPIPRTLAITAFGDMDVSIINQLPAGRKPIITRWVKHQQLPTV
                     LDWLERELEVGAQVYFISPLIEESEALDLKNAVDLQSDLQAHFGEQVTVDLLHGKMKN
                     DEKDAIMQAFKERKTNILVSTTVIEVGVNVPNATVMVIMDADRFGLSQLHQLRGRVGR
                     GHKQSYAVLVANPKTESGKERMKIMTETTDGFILAEADLKMRGSGEIFGTRQSGLPEF
                     QVANIIEDYPILEEARRVASQIVSVENWQEDPNWSILLANLKDREELD"
     gene            complement(2185..2256)
                     /locus_tag="ACHU66_002058"
     tRNA            complement(2185..2256)
                     /locus_tag="ACHU66_002058"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2221..2223),aa:Glu,seq:ttc)
     gene            complement(2279..2351)
                     /locus_tag="ACHU66_002059"
     tRNA            complement(2279..2351)
                     /locus_tag="ACHU66_002059"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2316..2318),aa:Asn,seq:gtt)
BASE COUNT          744 a          608 c          362 g          639 t
ORIGIN      
        1 caatatgtca aaagaccaac acaggtgtgt tggccttttt tatatgacta gtccaattcc
       61 tctcgatctt tcaaattagc aagcaaaatc gaccaattag ggtcctcctg ccaattttcc
      121 acgctaacaa tctgactagc aactcgtctt gcttcctcta aaattggata gtcctcgata
      181 atatttgcaa cctgaaattc tggcaaacct gattgacgag tcccaaatat ttctccagac
      241 ccacgcattt ttaagtctgc ttcagccaaa ataaaaccat ctgtcgtctc tgtcataatt
      301 ttcatgcgtt ccttaccaga ctctgtttta ggattagcaa ctaacactgc ataagactgc
      361 ttatgaccac gtcctacacg ccctctcaac tgatgtaatt ggcttaaacc aaaacgatct
      421 gcatccataa taaccatcac cgttgcattg ggcacattga ctccgacttc aataacagtg
      481 gtagaaacca aaatattcgt ttttcgctcc ttaaaagcct gcatgatggc atctttctcg
      541 tcattcttca tcttaccgtg taacaaatcc actgtaactt gctctccaaa atgcgcctgt
      601 aaatcagatt gcagatctac agcattcttt aaatctagag cctcagattc ttcaattagc
      661 ggagagataa aataaacctg agcacccact tctaactccc gctccaacca atctaagacc
      721 gtgggcagct gttgatgttt gacccagcga gtgataattg gcttccgacc agcaggtaac
      781 tgattaataa tcgatacatc catatctcca aaagctgtaa tggctaatgt ccgaggaata
      841 ggtgtagcag tcatcataag cacatccgga ttatcaccct tctcacgaaa cagacggcgc
      901 tgtttaacac caaaacgatg ttgctcatcc gtcacaacca aaccaagctt atggtacgtc
      961 acactttcct gtataagagc atgagttcct acaatgatgt ctacctgacc actagaaatc
     1021 gcctctaaag cagtacgacg ctccgcagcc ttcattccac ctgtcaaaag agcaatagaa
     1081 agttctggaa aaagctggcg caaactctca aagtgttgct cagccaaaat ttctgtcggc
     1141 accataattg cagcctgcat ccccgcagtc acagcagcaa acatagccaa tccagcaact
     1201 actgtcttcc ctgaaccaac atccccttgt aacaaacgat tcatgtgccc ataggacttc
     1261 atatctgata gtatttcagc caaagcccca gactgagcat ctgttaagac aaacggcaat
     1321 tgtcttattt gcatagccaa tctatcagca tcataggcaa tcttcaaacc attcgaaata
     1381 tcacgattgt tggctttcag catttgcaac tgtaattgaa aataaaataa ttcctcaaac
     1441 ttcatacgtc gcaaagcttg tctatactct tctaaatttg taggaaaatg catcgcaaaa
     1501 accgcctctc ggcgattcat taaccgatag cgctctcgta aaactgatgg caagttctcc
     1561 tctaataaat gcaaatatcc ctgatcaata gctgttttaa tagccttaac caaatttact
     1621 tgcgaaatcc cttgcgccac atgatagaca ggttggagct catctgatac ttgagccaaa
     1681 attttcattc ctgtcaaact tgccttagcc ttatcccact ttccccaaac tgcaatatcc
     1741 tgccccaaaa caatcttatc tgctaaataa ggctgattaa aaaacgaaac tgctaagaca
     1801 acctcaccct gtttcattga aaaacggagt ctatttcttt tgtaaccata atactgtaca
     1861 ttagctggag ataccacctc cccaactacc actgccttct ccccatcctg caaatcataa
     1921 atagacttac tctcaaaatc ttcatatcta aatggatagt aagtcagcaa atcattaata
     1981 ttctgaatat taatttttaa gaatttttca gccgattttg gaccaatccc tggcaaaacc
     2041 gataattcat cacataatct tttcatgtct ctattatatc aacaaacaat gaatagacaa
     2101 agtaacaaga aaagaaaaaa gagccaaatt aaagcccata cacctcaaaa aacaaaaaaa
     2161 agagacctaa gcagcctcca aatatagtcc gtacgggatt cgaacccgtg ttaccgccgt
     2221 gaaaaggcgg tgtcttaacc ccttgaccaa cggaccatat actaaagatt tctctttact
     2281 ccgccaacag ggctcgaacc tgtgacatca tgattaacag tcatgcgctc taccaactga
     2341 gctatggcgg aat
//
LOCUS       DAZQCJ010000157         2262 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000157 DAZQCJ010000000
VERSION     DAZQCJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.159"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            153..238
                     /locus_tag="ACHU66_002060"
     tRNA            153..238
                     /locus_tag="ACHU66_002060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Leu,seq:aag)
     gene            473..1354
                     /locus_tag="ACHU66_002061"
     CDS             473..1354
                     /locus_tag="ACHU66_002061"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR3390312.1"
                     /translation="MPLVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKQAP
                     VLIQTSMGAAKYMGGYKVARNLIANLIESMNITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLAKAKEVVELAHAKGISVEAEVGTIGGEEDGIVGAGELAPIEDAV
                     AMVATGIDFLAAGIGNIHGPYPANWEGLNLDHLRKLTEAVPGFPIVLHGGSGIPDAQI
                     QEAIKLGVAKVNVNTECQIAFANATRKFAAAYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSANKA"
     gene            1749..1937
                     /gene="rpmB"
                     /locus_tag="ACHU66_002062"
     CDS             1749..1937
                     /gene="rpmB"
                     /locus_tag="ACHU66_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR3390313.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
BASE COUNT          790 a          382 c          477 g          613 t
ORIGIN      
        1 taagcattat attttaagca ttatatagta gaatattgca aaagaatcag ctaaatcaat
       61 acgttttggc aaaaaaggta aaaataaaaa ataaaattca aaaaaagtgt tgacaaggct
      121 agttcctaat gatatactag ttcttgtgtt aagcggggat ggcggaattg gcagacgcgc
      181 aggactaagg atcctgtgac cgctttaggt cgtgtgggtt caagtcccac tctccgcata
      241 gtggactagc taaggctagt ccttttgctt tgtctagata agtgcagagc acgatatatg
      301 ctagttcata aattacaaga atgatacgaa acaggcatta ttatggcttt catgaaaaga
      361 tatgtgaaag ttttctcaaa actagacggt ttcaactagc attttcaaaa taaaaatggt
      421 agaatagaag agtatgaggt gcaacctcaa aaaatattag gaggctatcg aaatgccatt
      481 agtttcagca gaaaaatttg tccaagcagc gcgtgacaac ggttatgcag ttggtggatt
      541 taacacaaac aaccttgagt ggactcaagc tatcttgcgt gcagcagaag caaaacaagc
      601 tccagttctt atccaaactt ctatgggtgc agctaagtac atgggtggtt acaaagtagc
      661 tcgtaacttg attgcaaact tgattgaatc aatgaacatt acagttccag ttgctattca
      721 ccttgaccac ggtcactacg aagatgcact tgagtgtatc gaagttggtt acacttctat
      781 catgtttgac ggttcacacc ttccagtaga agaaaacctt gcaaaagcaa aagaagttgt
      841 agaattggcg cacgctaaag gtatctcagt tgaagctgaa gttggtacta tcggtggtga
      901 agaagatggt atcgtaggtg ctggtgaatt ggctccaatt gaagacgcag tagcaatggt
      961 tgcaactggt attgacttct tggcagcagg tatcggtaac atccacggtc catacccagc
     1021 aaactgggaa ggtcttaacc ttgaccactt gcgtaaattg actgaagctg taccaggttt
     1081 cccaatcgta ttgcacggtg gttcaggtat tccagatgca caaatccaag aagcaatcaa
     1141 attgggtgtt gctaaagtta acgtaaacac cgagtgccaa atcgcatttg caaacgcaac
     1201 tcgtaagttt gcagcagctt atgaagcaaa cgaagcagaa tacgataaga aaaaactctt
     1261 tgacccacgt aaattcttgg cagacggcgt gaaagctatc caagcatctg ttgaagagcg
     1321 tatcgacgta ttcggttcag cgaacaaagc ttaatgtaac tgcgaagaaa tctcagattt
     1381 caagcaaaca aagcataagt ttcttagatt aaaccaaaaa ggattttctt tcgagaatcc
     1441 tttttattat aaatagttga ttaagtttga tctagttctt tgccatgaag aaatctacga
     1501 tagaagtgag cagagaacag ttaaaatata aaacgctcca aagttttcat gactgtaata
     1561 acttagagaa cgaagaatta tggatggggc gcaaactgta gtattaaaat atttacctat
     1621 gaatatagat gctttaaaaa aataaaaatg aaaataattc ttgccaagtc caatcttttc
     1681 tgttagaata gtatggtatg tggtgctagc acatccgata tatttgatca aatgaggagg
     1741 aagatacaat ggctaaagta tgttatttca ctggtcgtaa gactgtatca ggtaacaacc
     1801 gttcacacgc tatgaaccaa actaaacgcg cagttaaacc aaaccttcaa aaagtaactg
     1861 ttttgattga tggtaaacct aaaaaagttt gggcttcagc acgtgcacta aaatcaggca
     1921 aagttgaacg cgtataataa caaagtcgtt agaaaaatct agcgactttt gtttttggaa
     1981 aaaatagcct agatataaag tcggtgagga gcaaattcgg ccactacata ataaacatga
     2041 aagaatatca gctatatagc tcttcacatt gctattgaaa agttaacaaa gaaggatcgt
     2101 tgtcaaaaat aaactgtgta ttgatgtgga agatttttcc aagttcgaac atttaaactt
     2161 ggattgacat taggagttgt acgagagagc atccgtacgg gttctacaag aaaggaatca
     2221 cgaactggaa gaatagagag tgtttaaaaa aatccaaaaa at
//
LOCUS       DAZQCJ010000158         2210 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000158 DAZQCJ010000000
VERSION     DAZQCJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.160"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            46..783
                     /locus_tag="ACHU66_002063"
                     /pseudo
     CDS             46..783
                     /locus_tag="ACHU66_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713635.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(834..2183)
                     /gene="gorA"
                     /locus_tag="ACHU66_002064"
     CDS             complement(834..2183)
                     /gene="gorA"
                     /locus_tag="ACHU66_002064"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR3390314.1"
                     /translation="MKVFDIIAIGGGSGGIATMNRAAMYGAKAAVIEGSYLGGTCVNL
                     GCVPKKIMWYGSQVAEAIQHYGPEYGFTSQEVTFDFATLRKNREAYIERSRASYGNTF
                     NNNGVEVIQGFARFVDNQTVEVNGELIRAKHIVIATGAKPVVPAIPGSELGIVSDDVF
                     AWEELPSSVAVIGAGYIAVEMAGLLHGLGVQTDLFVRGNRPLRSFDTYIVDALMEEMQ
                     RTNLPLHTGKTPVSLEKTDDGQIQINFADGSNHIAQKVLWAIGRKPNIDKLNLKATSV
                     QLTSSGHISVNEYQETAVPGIYALGDVTGEKELTPVAIKAGRLLAERLFNNKPTAKMD
                     YTTIPTVVFSHPAIGTVGLTEDEAITQYGQDNVKVYTSAFTSMYTALENNRQMAKFKL
                     VTVGENEKVVGLHGIGYGVDEMIQGFSVAIKMGATKEEFDAVVAIHPTGSEEFVTMR"
BASE COUNT          639 a          469 c          463 g          639 t
ORIGIN      
        1 cctataccta tgctagaata ataaaaaaag aaagtgagaa tttctatgac agaattactt
       61 gatatttacc gtactcttaa atctaaaact tgggtagact tgactcacca aatcaatgaa
      121 aagagcccga atttccctgc gctaccagct ctcgaaaaga aagccctatt tactcataag
      181 gatggcttct ttgtcgaaca atttacggtt gtggggcagt acggaaccca tcttgatcct
      241 ccgattcatt ttgtagaagg ggctcgttat cttgatgaga ttgatttgaa agatttactc
      301 ctgccactct acgttattga taaatcagct gctgttatag aaaataatga ctatgagata
      361 actaagcagg atattttaga ctttgaggct gaatatggtc cgatagcgcc aaaatctttt
      421 gttgcctttc gttcggattt gtcaaagcgc tggcccagtc aagatgccat tcgtaatcta
      481 gacgaagatg gagttcaacg tacaccagga tggagtcatg aagctctaga atatttgatt
      541 gaagaacgtc aggtgaaagc agttggtcat gaaacattgg ataccgattt aggagtatct
      601 gcagccaaac acggaggaag tttatcagaa gaatactatc tcctatcaaa agatatttat
      661 cagttggaag tattggcaaa cttggattag gtaccgccaa caggtgcatt gatttcaatc
      721 gcttttcctc attgggagaa ggcttcgggt tcaccagtgc gagcaattgc catattacca
      781 taaaaaaaag tccagacagt gctgaccttc atgtctggac tttttcaatt gagttaacgc
      841 atagtaacaa attcttcaga acctgtcgga tggatggcga caacggcatc aaattcttct
      901 tttgtagctc ccattttgat agcaactgag aatccttgaa tcatttcatc gacaccatag
      961 ccaatgccgt gtagtccaac gactttttca ttttctccca cagttactaa tttaaactta
     1021 gccatttggc gattgttttc tagggcagtg tacatagaag taaaggctga agtatagact
     1081 tttacattgt cctgaccgta ttgggtaatc gcttcgtcct cagttagacc aacggttccg
     1141 atagcggggt gagaaaagac gacagttggg atggttgtgt aatccatttt tgcggttggt
     1201 ttattgttaa ataaacgttc tgccaaaagg cgacctgcct tgattgcaac gggtgttaat
     1261 tctttttcac cagttacatc accgagggca tagatgcctg gtacagctgt ttcttggtat
     1321 tcattgacag aaatatgtcc tgaagaagta agttgaacac tagttgcttt caggttgagt
     1381 ttgtcaatat ttggtttacg tccaattgcc caaaggacct tctgagcaat atggttacta
     1441 ccgtcagcaa agttaatctg gatttgtcca tcatcagttt tttcaagact gacaggagtt
     1501 ttcccggtat ggagtggaag gttggtacgt tgcatttctt ccatcaaggc atcaacaatg
     1561 taggtatcaa agctacgaag agggcgatta ccacgaacaa ataggtcagt ttggacaccg
     1621 agtccatgta ggagaccggc catttccaca gcgatataac cagcaccaat cacagccaca
     1681 gaagagggta attcttccca tgcaaagaca tcgtcagata caatccctag ctcactacca
     1741 ggaatagcag ggactaccgg tttggcgcca gttgcgatga caatgtgttt tgcacgaatc
     1801 agttcaccat tgacttcaac cgtttgatta tccacaaaac gtgcgaaacc ctggataact
     1861 tctactccgt tattgttaaa agtattaccg taggatgcgc gtgaacgctc aatataggct
     1921 tcgcgatttt tacgcaaggt ggcaaaatca aaggtcactt cttggctagt aaatccatat
     1981 tcaggtccat aatgctgaat ggcttctgct acctgagagc cgtaccacat aatttttttg
     2041 ggtacacagc caaggttaac acaagtccct cctagatagc ttccctcaat aactgctgcc
     2101 ttggcaccgt acatagcagc tcggttcatg gtagcgatgc ctccgctccc accaccgata
     2161 gcgataatat caaacacttt cataaatttc ctccaataaa atcattaact
//
LOCUS       DAZQCJ010000159         2197 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000159 DAZQCJ010000000
VERSION     DAZQCJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.161"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            5..1852
                     /gene="typA"
                     /locus_tag="ACHU66_002065"
     CDS             5..1852
                     /gene="typA"
                     /locus_tag="ACHU66_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFR3390315.1"
                     /translation="MTKLREDIRNVAIIAHVDHGKTTLVDELLKQSQTLDERTQLDER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLTPIVVVNKIDKPSARPEEVVDEVLELFIELGADDDQ
                     LEFPVVYASAINGTSSLSDNPADQEETMAPIFDTIIEHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERKEIQEAKAG
                     DLIAVSGMEDIFVGETVTPTDAVEALPVLRIDEPTLQMTFLVNNSPFAGREGKWVTSR
                     KIEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVKMEPFERVQIDTPEEYQGSVIQSLSERKGDMLDMVATGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIDQGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITTAKQMTNVRSATKDQTSVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAERDKAAKKKKLATEE"
     gene            1870..2118
                     /locus_tag="ACHU66_002066"
     CDS             1870..2118
                     /locus_tag="ACHU66_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HFR3390316.1"
                     /translation="MFYLILAIMLVLFYIFIAPRNIKGTMNLIAAVFLLVALAIALVL
                     GFLRIMQFPKEIWVGLLMILIGFWAMRDIYQLNDDTKF"
BASE COUNT          632 a          438 c          495 g          632 t
ORIGIN      
        1 tattatgaca aaacttagag aagacatccg taacgttgcc attattgccc acgttgacca
       61 cggaaaaaca accctcgttg atgaattatt gaaacaatcc caaacacttg atgaacgtac
      121 gcagttggat gagcgtgcca tggactcaaa cgatatcgaa aaagagcgtg gtatcacaat
      181 cctggctaaa aacactgccg tagcttacaa cggtactcgt atcaacatca tggacacacc
      241 aggacacgcg gacttcggtg gtgaggttga gcgtatcatg aaaatggttg acggtgttgt
      301 ccttgtagta gatgcttacg aaggtaccat gccacagact cgtttcgtat tgaaaaaagc
      361 ccttgaacaa aacttgacac caatcgtcgt tgttaacaag attgacaagc cttctgctcg
      421 tccagaagaa gttgtggatg aagtgcttga actcttcatc gaattgggtg cagatgatga
      481 ccagttggaa ttcccagtgg tttatgcatc agctatcaac ggaacgtcat ctttgtctga
      541 caatccagca gaccaagaag aaacaatggc accaattttt gataccatta tcgagcatat
      601 tccagcgcca gtcgataact cagatgagcc tttgcagttc caagtatcgc ttttggacta
      661 caatgacttc gtaggtcgta tcggtatcgg tcgtgtcttc cgtggtactg ttaaagttgg
      721 tgaccaggtt accctttcta aactagacgg tacaacgaaa aacttccgcg ttacaaaact
      781 ctttggtttc ttcggtcttg agcgtaaaga aatccaagaa gcaaaagctg gtgatttgat
      841 tgctgtttct ggtatggaag acatcttcgt tggtgaaact gtgactccga cagatgcagt
      901 tgaggcactt ccagttcttc gtatcgatga gccaactctt caaatgactt tcttggtgaa
      961 caactcaccg tttgcaggtc gtgaaggtaa atgggtgaca tctcgcaaga ttgaagagcg
     1021 ccttttggcc gagttgcaaa cagacgtttc tctccgtgtt gaccctactg actctccaga
     1081 taaatggact gtttcaggtc gtggtgaatt gcacttgtct atcttgattg aaaccatgcg
     1141 tcgtgagggt tatgagcttc aagtatcacg tccagaagtt atcatcaagg aaatcgacgg
     1201 cgtgaaaatg gagccatttg agcgcgtgca aattgataca ccagaagaat accaaggttc
     1261 tgttatccaa tccctatctg agcgtaaggg tgatatgttg gatatggttg cgacaggtaa
     1321 tggtcaaaca cgacttgtct tcctcgtacc tgcacgtggt ttgattggtt attcaacaga
     1381 attcctttca atgactcgtg gttacggtat tatgaaccat acctttgatc aatacttgcc
     1441 agttgttcaa ggtgaaatcg gtggtcgtca ccgtggtgct ctcgtttcta tcgatcaagg
     1501 taaggcaacg acatactcta tcatgcgtat tgaagagcgt ggtaccatct ttgtaaaccc
     1561 aggtactgaa gtgtacgaag gaatgattat cggtgaaaac tcgcgtgaaa atgacttgac
     1621 cgttaacatc acgactgcta aacaaatgac caacgttcgt tcagcaacca aagaccaaac
     1681 gtctgtaatt aagacaccac gtatcttgac tcttgaagaa tcactagaat tcttgaacga
     1741 tgatgagtac atggaagtaa cgccagaatc tatccgtctt cgtaagcaaa tcttgaacaa
     1801 ggcagagcgt gataaggctg cgaagaagaa aaaattagcg actgaggaat aatttctagg
     1861 agaagaagca tgttttattt aatcctcgcc atcatgttag tactttttta tatttttatt
     1921 gcacctcgca atattaaggg gactatgaat ttgattgcgg cagtattttt actggtagca
     1981 ttagcgattg ccttggtact tggattttta agaatcatgc aatttccaaa ggaaatttgg
     2041 gttggtctac taatgattct gattggtttt tgggcgatgc gggatattta tcaattaaat
     2101 gacgatacaa aattttagca gagtctttga tttttgaata ccgtaatacg atattactaa
     2161 ggaattttat ccagcttttt ggacagtagt ggcaatc
//
LOCUS       DAZQCJ010000160         2159 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000160 DAZQCJ010000000
VERSION     DAZQCJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.162"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            127..777
                     /locus_tag="ACHU66_002067"
     CDS             127..777
                     /locus_tag="ACHU66_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR3390317.1"
                     /translation="MSRILVVEDDIVISQVVCEFLKEHGYQVESVFDGKVALERFQEE
                     QFDLIVLDIMIPSMTGLEVLKEIRKTSQIPILMLTAMGDEYTQLISFNQIISDYVVKP
                     FSPTILVKRIENILRGKGETDSIEIGTILIQPTSGAVYMEEEEVQLTKKEYEVLLYLA
                     KRRGKIVSRDNLMMGIWGYTELDSRVLDNHIKNIRKKLPSLPLKTIVGRGYQIEGT"
     gene            779..>2159
                     /locus_tag="ACHU66_002068"
     CDS             779..>2159
                     /locus_tag="ACHU66_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR3390318.1"
                     /translation="MGIARKNFIIVAAMISFISVTLLGLLYYAMPIYYNQVKKQELRH
                     DYMTVAKQLDGMPEAKIISEIDDFDIKTPNIIISLFSSDRKIIYPDPNDEMAKKHENE
                     YLENGDFDEIGSWGAEITSAEGVTYYLLFNYGFHSLSDVSQTLVTFYPFILLLIIVLA
                     VSVAFVYSRLSTRRIARISETTRRMQSLESGISCAVAGTDEITILAQDINSLYSKLLS
                     SIDELRTENERAMAREKEKSDFLRITSHELKTPIASMLGLVEGMIYNVGPFKDHDTYL
                     KKCKEILQEQSELVHSILEATNLDMALKESREQIRLDQLIDSSLTSYQSLAEVKGYQF
                     EVELSPVLIEANPVYFLKAIKNILDNAFRYSCKEAIIRVVLRQNQLIIENQVERVLSD
                     EELDQVFRPFYRPDYSRDKKDGGIGIGLFIVQKILEKHGFPYTFYASDTQTMRFMIHL
                     QSQSTANSNE"
BASE COUNT          723 a          287 c          456 g          693 t
ORIGIN      
        1 aagaaagctg aaatagcaga acatggtgct ctatttatct atgatagagt tgcaccattg
       61 tacgaatcgg tatataattg aagtataata atattttata atagaacttt acgaggaggt
      121 ataaaaatgt caaggatttt ggttgtagag gatgacatag ttattagtca agttgtttgt
      181 gagtttttaa aagaacatgg ttatcaggta gaatctgttt ttgatggaaa ggttgcttta
      241 gaaaggtttc aagaagaaca attcgattta attgttttag atatcatgat tccatctatg
      301 acaggtttgg aagtactgaa ggaaattcgc aaaacttctc agattccaat tttgatgctg
      361 acagccatgg gtgacgaata tacacagctt attagtttta atcagattat aagtgattat
      421 gttgttaaac cattttcacc aactatattg gtaaaacgga ttgagaatat tttgagagga
      481 aaaggagaga cagatagcat tgagatagga acaattctta ttcaaccaac tagcggggca
      541 gtttatatgg aagaagaaga agttcaattg acgaaaaaag aatatgaagt tttactatat
      601 ttagctaaac gacgtgggaa aattgttagt cgtgataact tgatgatggg aatatgggga
      661 tatacggaat tggatagtcg tgtcttagat aatcatatca agaatatccg taagaaattg
      721 ccgtcacttc ctttgaagac aatagttggt cgtggttatc aaatagaggg tacttaatat
      781 ggggattgcc agaaaaaatt ttattattgt tgctgcgatg atttctttca tctctgtaac
      841 attgttaggg ttattgtatt atgctatgcc tatatattat aatcaggtta agaaacagga
      901 attgagacac gattatatga ctgtggctaa acaattggat gggatgccag aagctaagat
      961 tatatctgag attgatgatt tcgacataaa aactccgaat attattatta gtcttttttc
     1021 gagtgataga aaaattattt atccagaccc caatgatgaa atggctaaaa agcatgagaa
     1081 cgagtattta gaaaatgggg attttgatga aattggttct tggggagccg agataacatc
     1141 tgcagaagga gttacatatt atctgttatt taattatgga tttcactcgt tatctgatgt
     1201 cagtcaaaca ttggtaactt tttatccatt catattgctg ttgattattg ttctggcagt
     1261 ttcggtagct tttgtttaca gtagactgtc cactcgtaga atagcaagaa tttcagaaac
     1321 aacaagacga atgcaatcgt tagagtctgg aatcagttgt gctgttgcag gaactgatga
     1381 aataacaata ttggctcagg atattaacag cctgtatagc aagttattgt caagtatcga
     1441 tgaattaaga acggaaaatg aacgtgctat ggcacgtgag aaggagaagt ctgatttcct
     1501 ccgtatcaca tcacacgaat taaaaactcc aatagctagt atgttggggt tagtcgaagg
     1561 catgatttat aatgtggggc catttaagga ccatgataca tatttaaaaa aatgcaagga
     1621 aatcttgcag gaacaatctg agttagttca ttctatttta gaagcgacaa atcttgatat
     1681 ggctttaaag gaaagcagag aacaaatccg attagatcag ttgattgatt cctctttaac
     1741 aagttatcag agtttggcag aggttaaggg ctatcaattt gaagtagaac tatcaccggt
     1801 tctcattgag gcaaatcctg tttatttctt aaaagctatt aagaatattt tagataatgc
     1861 ctttcggtac tcatgtaaag aggctattat tcgtgtagta ctacgccaaa atcaattaat
     1921 aatcgaaaat caggttgaac gtgttttaag tgacgaggaa ttggatcaag tctttcgtcc
     1981 attctatcgt ccagactaca gtcgtgataa gaaagatggt ggcataggga ttggtttgtt
     2041 tattgtacag aaaatattag aaaaacacgg ctttccgtac actttttatg cttctgatac
     2101 acaaactatg cgttttatga ttcatttgca atcacaaagt acagcgaata gtaatgaat
//
LOCUS       DAZQCJ010000161         2137 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000161 DAZQCJ010000000
VERSION     DAZQCJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.163"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(17..556)
                     /locus_tag="ACHU66_002069"
     CDS             complement(17..556)
                     /locus_tag="ACHU66_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HFR3390319.1"
                     /translation="MTRVAVVLADGFEEIEALAPVDVFRRAHFDCQIVGLDSHEVEGS
                     HGIRVQTDQVFDGDLSSFDLIVLPGGMPGSVHLRDSESLITELQRAVASGKSVAAICA
                     APIVLDKAGLLDSRHYTCFPGKEKDIPSGILLEEDVVVDGPIITSRGAGTSLDFAYKL
                     VDLFGGDGESLAQTMVYHS"
     gene            complement(570..1799)
                     /locus_tag="ACHU66_002070"
     CDS             complement(570..1799)
                     /locus_tag="ACHU66_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFR3390320.1"
                     /translation="MRKLMKNRGTIDSRVDYSLILPVFFLLVVGIISLYIAVSQDYPD
                     NVVQMVGQQIAWIGIGTVAAFLVMFFSTKFLWQITPYLYVLGLGLMVLPLFFYSPDLV
                     ASTGAKNWVTIGGMTLFQPSEFMKIAYIIMLSRVIVTFHKYYPNRKIREDFMLIGYMT
                     LFTIPVLILLALQKDLGTSLVFVAIFSGMLLLSGVSWKILLPTALTGIVLVGGFMLIF
                     ISPGGTTFLHNLGMDTYKINRIAAWLDPFKNAQSTTYQQAQSLIAIGSGGLKGLGFNK
                     TNLLIPVRESDMIFTVIGEDFGFIGGTVLIGLYLLLIYRMLRVTLKSNNRYYTYISTG
                     YIMMLLFHVFENVGAATGLLPLTGIPLPFISQGGSSMVSNLIGVGLVLSMGYQSRLAD
                     EKETNRSRRYRKITIKR"
BASE COUNT          719 a          488 c          354 g          576 t
ORIGIN      
        1 catcttcttc attactttaa ctatgataaa ccattgtttg agctaggctt tccccatctc
       61 caccaaataa atcaaccaat ttataggcaa aatccaaact tgttccagca cctcggctag
      121 tgataattgg accatcaacg accacatcct cttccagaag aatacctgac ggaatgtctt
      181 tttctttccc tgggaaacat gtatagtgac gactatctaa caaaccggcc ttatccagaa
      241 caattggagc cgcacagatt gctgctactg attttcctga tgcaaccgct ctttgcaatt
      301 ccgtaattag tgactctgaa tctctaaggt gaacagaacc tggcattcct cctggtaaaa
      361 caatcagatc aaaggaagac aggtctccat caaagacttg gtcagtttga acacgaatac
      421 cgtgcgatcc ctccacttca tgactatcta aaccaacaat ctgacagtca aaatgagctc
      481 gacggaatac atctacaggg gccaatgctt cgatttcttc aaaaccatcg gctaaaacaa
      541 ctgcaactct tgtcatataa accaactttc taacgtttta tcgtaatttt tctatagcga
      601 cgtgatcgat ttgtttcctt ctcatcagct agtcgtgact gataccccat tgacagcact
      661 agaccaacac caatcaaatt tgaaaccatg gacgagccac cttgggagat aaacggcaaa
      721 ggaatacctg ttaaaggcaa caaacccgtt gcagcgccaa cattttcaaa cacatggaac
      781 aacagcatca tgatatagcc agttgatatg taggtgtagt atcgattgtt tgacttcaac
      841 gttacccgca gcattcgata aatgagcaac aagtatagtc caatcaatac cgtcccacca
      901 ataaaaccaa agtcctctcc aatcaccgta aaaatcatat cactttctcg aacaggtata
      961 agaagatttg ttttattgaa acctagtcct tttaggccac cacttccgat agctatcaaa
     1021 ctttgagctt gctggtaggt cgttgattgg gcatttttaa agggatcaag ccaggctgca
     1081 atacgattga ttttataagt atccatcccc aaattgtgga gaaatgttgt tccaccagga
     1141 gaaataaaaa tcagcataaa tccaccaacc aaaacgatcc ccgttaaggc agtcggtaat
     1201 agaattttcc aagaaactcc agataataga agcattccgc taaagattgc tacaaatact
     1261 aaggaggttc ctaaatcttt ctgtaaggcc aacaaaatca ggacggggat agtaaacagt
     1321 gtcatataac caatcaacat aaaatcctca cgaatttttc gatttggata atatttatga
     1381 aaggtgacaa tcacccttga caacataatg atgtaggcta ttttcataaa ttcggatggt
     1441 tgaaaaagag tcattccacc aatcgttacc cagttttttg ctcccgttga cgctactaag
     1501 tctggactgt agaaaaacaa aggcaatacc ataagtccta gcccaagtac gtacaagtaa
     1561 ggtgtgattt gccacagaaa cttagtggaa aagaacatga ccaaaaaagc tgccactgtt
     1621 ccaattccta tccaagcaat ttgttgacca accatctgta caacattatc cggataatct
     1681 tgactaaccg caatgtagag agaaataatc cccacaacta ataaaaaaaa gaccggtaaa
     1741 ataagtgagt agtctactcg actatcgatt gttcccctat ttttcatcaa tttcctcata
     1801 ctttcacttc aaattcttac tcgttccatt atagcagaaa tcatctctca ttgaaactaa
     1861 aaatctagtt acatcagcgg ggatacgatt tatactcaac gaaaatcaaa agtagcctag
     1921 gaaacgaagt cgaagataga actggagttc atcaaggcaa gttgacaacg gagaattttg
     1981 attttcgaag agtattacta gtgcacaaat cataaaatac gtatatcttc ataccttcga
     2041 acaggctgaa atataatgct ttaccttcac acaccccata agtttgctca attcatgagt
     2101 atgtaaacca atggcaatca ttttggcaat cacgcac
//
LOCUS       DAZQCJ010000162         2126 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000162 DAZQCJ010000000
VERSION     DAZQCJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.164"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..439
                     /locus_tag="ACHU66_002071"
     CDS             <1..439
                     /locus_tag="ACHU66_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR3390321.1"
                     /translation="LSLECRDIMSSKKINFQIVNESHTRVIKGNRLNLDRALINILSN
                     ATRFCSEEGKVVLTISESSNFISFEIWNNGLPFTEESLKDGGKLFYTDNKGRNDKHYG
                     IGLSFAQKIAMRHQGQLILSNPQNGGAQVTLLIKKILRAGQKA"
     gene            611..1312
                     /locus_tag="ACHU66_002072"
     CDS             611..1312
                     /locus_tag="ACHU66_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3390322.1"
                     /translation="MEVIIQNLRKSYKDKEVLKDVSFKIEEGTICGLLGVNGAGKSTI
                     MKILFGLERADSGEVIFNGKHKDIVEENRFDIGALIESPAIYLNLSAFDNLKTRALLH
                     DIPDERICEVLQLVGLSDTGKKKAGHFSLGMKQRLGLGMAIITNPDLLILDEPTNGLD
                     PDGIKELLNLIRDLKKSGMTVLLSSHQLSEVSKIADQIVILNQGKIFYDDINKNSNDL
                     EKLFFRIVHGGDGND"
     gene            1305..2042
                     /locus_tag="ACHU66_002073"
     CDS             1305..2042
                     /locus_tag="ACHU66_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter permease"
                     /protein_id="HFR3390323.1"
                     /translation="MTNIFKSEWLKQKSSSDKKLLILIPLLAIFIAFILAGPLNLESF
                     SIYWWEGGFLFTLLGLLFLADYSAEENAGAFQNISLGRYTFTIYLAKILLKFKDVLIS
                     TFIFTIIFYGISFLFSGVISVDIGKDLICLLLIGVSSAWVLPLLYFLAKWINPYLLLA
                     TNSLICLLVAPLIAQTSVWFIFPYTYHYKIAYALLHLKPSGDLEAALSGVEISTILIS
                     VSLSILVFVSFLSLLKWRIINEQNSKK"
BASE COUNT          702 a          286 c          383 g          755 t
ORIGIN      
        1 attatcatta gaatgtcgag atattatgtc ttcaaaaaaa attaattttc aaatagttaa
       61 tgaatcccat accagagtga ttaaaggaaa tcgtctcaac ttagatcgag ctttgataaa
      121 tatcttatct aatgcgacta gattttgttc agaagagggc aaggtggtac tgactatatc
      181 tgaaagttct aactttatca gttttgagat ttggaataat ggtttaccat ttacagaaga
      241 gtctcttaaa gatggaggaa aactttttta cacagataat aaaggaagaa atgataagca
      301 ttacggaata ggactttcat ttgctcaaaa gatagctatg agacatcaag gtcaattaat
      361 actctcaaat ccacaaaatg gtggtgcaca ggtcacttta ttaattaaaa aaattttacg
      421 ggctggtcaa aaagcctaat ataaacaaaa acgcatgaaa tctttcattt acaaacgttg
      481 attttatgcg tttttttgtt tcgaaaattt gaaaaaatca atgagatatc gactttttgc
      541 tcaatctttt tttctgaaca aatactgaaa tttcttttgt attataataa aagaaatgga
      601 ggcagaaaaa atggaagtta tcattcaaaa tctaagaaaa agctataaag acaaagaggt
      661 tttaaaagac gtttcattta aaattgaaga aggtactatt tgtggtcttc tgggagtaaa
      721 tggggctggg aaatccacca ttatgaaaat tctttttggg ttagaaagag ctgatagtgg
      781 agaagtcatt ttcaatggaa agcataagga cattgtagaa gaaaatagat ttgatattgg
      841 agcgttgatt gaatctcctg ctatctattt gaatctttct gcatttgaca atttaaaaac
      901 acgagcattg ctacatgata ttccagatga aagaatttgt gaggtacttc agttggttgg
      961 tttatctgat actggaaaaa agaaggcagg gcacttttca ttaggaatga agcaacgact
     1021 tggtttagga atggcaatta ttaccaatcc tgatttatta attttagatg agccaactaa
     1081 tggtctagat ccagatggga taaaagaatt gctaaattta attagggatc taaaaaaatc
     1141 tggaatgaca gtcctcttgt ccagccatca actaagtgaa gtcagcaaaa ttgcggatca
     1201 aatcgttata ctgaatcagg gcaagatttt ttacgatgat attaataaga attcaaatga
     1261 tttagaaaaa ctattcttta gaattgtcca tggaggtgat ggaaatgact aatattttta
     1321 aatctgagtg gctgaagcaa aaatctagtt ctgataaaaa attactaatc cttattcctt
     1381 tgctagcaat ttttatagct ttcatactag caggacctct caatttagaa agtttttcaa
     1441 tttattggtg ggagggagga ttccttttca ctctccttgg gttattgttt ttagctgatt
     1501 atagcgcgga ggagaatgca ggtgcgtttc aaaatattag cttgggtaga tatacgttta
     1561 ctatttattt agcgaagata ctgttgaagt tcaaggatgt tttgatttca acctttattt
     1621 ttacgataat attttatggc atctcatttc ttttttcagg tgtgatatca gtagacattg
     1681 gtaaagattt gatttgttta ttacttattg gtgtttcttc tgcttgggta ttaccactat
     1741 tatattttct agcaaagtgg ataaatccct acttgttgtt ggctacaaat tctcttattt
     1801 gcttattagt tgctcctcta atagctcaga catcggtttg gttcatattt ccttatactt
     1861 atcactataa aattgcttat gctcttttac atttaaaacc ctcaggtgat ttagaggcag
     1921 cattaagtgg agttgaaata tcgacaattt taatatcagt atctctttca atcttagttt
     1981 ttgtgagttt ccttagtttg ttgaagtgga ggataattaa tgaacaaaat tctaaaaagt
     2041 gagttgctaa agttgaaagg ctctcttact ttaaatctta ttttaatttt atcaattatt
     2101 caattattca caattcctct ttattt
//
LOCUS       DAZQCJ010000163         2093 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000163 DAZQCJ010000000
VERSION     DAZQCJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.165"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..>2093
                     /gene="cas9"
                     /locus_tag="ACHU66_002074"
                     /gene_synonym="csn1"
     CDS             <1..>2093
                     /gene="cas9"
                     /locus_tag="ACHU66_002074"
                     /gene_synonym="csn1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962169.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HFR3390324.1"
                     /translation="LSQEQKEFLVNYAKETSVLGAGKILQQIAKLADCKVEDIRGYRL
                     DNKDKPELHTFETYRAMKGLVPLVDIGVLSREQLDILADILTLNTDFEGIREALKKQL
                     PNVFDEKQVKGLASFRKSKSQLFAKGWHNLSQKIMLEVIPELYATSDEQMTILTRLGK
                     FEKSSVAEYPSSINVDEITDEIYNPVVAKSIRQTIKIINASIKKWGEFDQIVIEMPRD
                     RNEDEEKKRIADGQKANAKEKADSILRAAELYCAGKVLPDYVYNGHNQLATKIRLWYQ
                     QGERCIYTGQPISIHDLIHNQNQYEIDHILPLSLTFDDSLSNKVLVLATANQEKAQRT
                     PYNYLKSATSAWSYREFKDYVTKRKGIGKKKCEYLTFEEDINGFEVRSKFIQRNLVDT
                     RYASKVILNALQDYFKISGIQTKVSVVRGQFTSQLRHKWGIEKTRETYHHHAVDALII
                     AASSQLRLWKKQESPLVVDYQEGRQVDLETGEILELTDEQYKELVYQPPYQGFVNTIS
                     SSAFDNEILFSYQVDSKVNRKISDATIYATRNAQLGKDKTEGIYVLGKIKDIYTQAGY
                     EAFLKRYTKDKTSFLMYHKDLDTWEKVIEIILRDYREYDEKGKEIGNPFERYRRENGY
                     VKKYSRKGNGTAIKSLKYYDNKLGNHIDITPENSRNAVVLQSLKPWRTDVYFNKETGK
                     YEFLGIKYSDLSFEK"
BASE COUNT          771 a          303 c          431 g          588 t
ORIGIN      
        1 agttaagtca ggaacaaaaa gaatttttag taaattatgc taaagaaacg tcggtattag
       61 gggctggaaa aatattgcaa caaattgcga aacttgcgga ttgtaaggtt gaagatatta
      121 gggggtatcg tttagataat aaggataaac ccgaactaca tacctttgaa acatatcgag
      181 caatgaaagg tctagttccg ttagtagaca ttggagtatt gtcgagagag cagcttgata
      241 tattagccga tattctaacc cttaatactg attttgaagg aattagagag gccctaaaaa
      301 aacaattgcc aaatgtattt gatgaaaaac aggtcaaggg gttagcaagt tttagaaagt
      361 cgaaaagcca attatttgca aaaggctggc acaatctatc tcaaaaaatt atgctagagg
      421 taattccaga attatatgca acatctgatg aacaaatgac aatcttaaca cgattaggaa
      481 agtttgagaa gagtagtgtt gctgaatatc cttcctcaat taatgtagat gagattacag
      541 atgaaatata taatccagtc gttgctaaat ctatccgtca aaccattaaa attatcaatg
      601 cttcgataaa aaaatggggt gaatttgatc aaattgttat agagatgcct cgtgatcgga
      661 atgaagatga agagaagaag cgaattgcgg atggacagaa agcaaatgca aaagaaaaag
      721 cggattcaat cctacgtgcg gcagaattgt attgtgctgg aaaggtattg ccagattatg
      781 tctataatgg acacaatcag ttagcaacaa aaattcgtct ctggtatcaa caaggagagc
      841 gttgtattta tactggtcag ccgatctcaa ttcatgattt aattcataat cagaatcagt
      901 atgaaattga ccatatcttg ccactttctt tgacatttga tgatagttta tcgaataagg
      961 ttttagttct tgcaactgca aaccaagaga aagctcaacg tacaccgtat aattatctga
     1021 aaagtgctac gtcagcttgg tcctatcgtg aatttaagga ctatgttact aagcgcaaag
     1081 gaattgggaa gaagaaatgt gaatatctga catttgagga agatattaat ggatttgagg
     1141 tgagaagtaa gttcattcag cgtaacttag tggatactcg atacgcctct aaagtcattc
     1201 taaatgcctt acaagattat ttcaaaatat cagggataca aacgaaagtt tctgttgtaa
     1261 gaggtcaatt tacatctcaa ttgcgtcata aatggggaat tgaaaagaca cgtgaaacgt
     1321 atcatcacca tgcggtggat gccttaatta ttgcagcatc aagtcaatta agactctgga
     1381 aaaagcaaga aagcccgctt gttgttgatt accaggaagg acgtcaagta gacttagaaa
     1441 ctggagaaat cttagaattg acagatgagc aatataaaga acttgtctat caaccgccgt
     1501 atcaaggatt tgtaaatact atctctagct cggcttttga taatgagata ctgttttctt
     1561 accaagtaga ctccaaggtg aatcgtaaaa tttctgatgc aaccatttat gcaactcgta
     1621 acgcacaact tggaaaagat aaaacggaag ggatatatgt ccttggaaaa atcaaagata
     1681 tctatacaca agctggatat gaggcatttt tgaaacgtta tacgaaggac aaaacttctt
     1741 ttctaatgta tcataaagac cttgatactt gggaaaaagt gattgagatt attttacgtg
     1801 actatagaga atatgatgaa aaagggaaag aaataggtaa cccatttgaa agatatcgta
     1861 gggaaaatgg ttatgtgaaa aaatatagcc gtaaaggaaa tggtactgca ataaaatcat
     1921 taaaatacta tgataataag cttggaaatc atatcgatat tactccagaa aatagtcgaa
     1981 atgcggtagt attgcagtca ttaaaacctt ggaggacaga tgtatatttt aataaagaaa
     2041 caggcaagta tgaattcttg gggattaaat attctgatct atcgttcgaa aaa
//
LOCUS       DAZQCJ010000164         2064 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000164 DAZQCJ010000000
VERSION     DAZQCJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.166"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(727..1491)
                     /locus_tag="ACHU66_002075"
     CDS             complement(727..1491)
                     /locus_tag="ACHU66_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390325.1"
                     /translation="MKFLLMNVRRTVFSKKMLLSFLLAFSCILIGNMVAFNGIYKLEG
                     LSLFFAGSTIRGFSPISLVAAVISAIPIADRVIEDSKNHFLRLQLQRTSRIKYIWTLL
                     VTAGISGFLSLFLPYFLLLVANLCLTPYKEIYIGDYQGVFKSIFDSNQLVYSILITIW
                     YGIFGSVFAVFGLASSLAFRQKIVGVFFPCLYMILGGLFFALLDLSFLEPVGIISWGY
                     QFQLNFLLVFLHLLCIFTICLGMILYHFQFRVEDSI"
     gene            complement(1576..1986)
                     /locus_tag="ACHU66_002076"
     CDS             complement(1576..1986)
                     /locus_tag="ACHU66_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390326.1"
                     /translation="MKTRLSIKKPIIVAIALLGLVSPTVFSRGVLDYSMVLPRIGSTT
                     TGSIVKVTHSRGVNRNDSTGGGKTLNTSIRNYHSGSDITPTYSLPSGARVLMSYHGSG
                     SHYVNETTTLGLATQFGNTVRVQTTGSWSPDEEQ"
BASE COUNT          822 a          360 c          333 g          549 t
ORIGIN      
        1 ggtttaacaa aatgaaaaaa agcaagagaa ttagccaata caataactcc ttgtggtaac
       61 ttactgattt taaaatatag tgatggatag gaaagacttg actaatggtt gcattgatcg
      121 gataaaacca gaaaacagat tgtattaaca aaaatgagaa aagattagcc atgactgtac
      181 ttcctttttg aaaaagaaga aaattaaaca aggctatttc cgagaggaaa ataagataac
      241 ataaaaaact aaacaagggc aaaagtatac tttgaaaatt agaaacgtaa gatatttcat
      301 aaacttctga tagaagtggt tgatgtatag aagaaattga aaaagaaaac gaatttacca
      361 accccatcaa aaataccaga cacatcagaa gaaatacaaa taagaatgga agtagcaagc
      421 tataatttaa taaaaagtaa tacttatctt tgatggataa aaaacgcagt gtgacaaatg
      481 tatctcttgt aaaaacttct atacaaaaaa ataaaatagc agtgtagata atcggtatca
      541 ggtagacacc tgttgttata tcttggcata gaatcagtag ccaggtaaaa acatttggaa
      601 caagttgata atgacctaaa taatattgat aggtggtatt tgaaaaaatc aaactcccaa
      661 gtaagatgat aaaaaataat ctgaagatag tagatttttt atgaaaaaat agaattctac
      721 aaaatctcat atactatcct ccactcggaa ctgaaaatga tataaaatca ttccaagaca
      781 gatggtaaaa atacacagta gatgcaaaaa tacaagtaga aaatttaatt gaaactggta
      841 accccaagaa ataatcccaa caggctctaa aaaagacaaa tctagcaagg caaaaaacaa
      901 cccgcccaaa atcatataga ggcaagggaa aaatacccca acaatttttt gacgaaaagc
      961 aagagaacta gctaggccaa atactgcaaa aacgctacca aaaatcccat accatatcgt
     1021 tattaagata gaataaacta actgattact atcaaaaata gacttaaaaa caccttgata
     1081 gtcaccaata tatatttcct tatatggcgt cagacataga tttgcaacca aaagcaggaa
     1141 atagggtaaa aaaagggaca aaaatcctga aattcctgcc gtcacaagaa gagtccaaat
     1201 atattttata cgggatgttc tttgtaattg caggcgcaga aagtgattct tactatcctc
     1261 aataacccga tcagctatcg gaatcgcact gataactgct gccacaaggc ttatcggcga
     1321 aaaacccctg atagtcgatc ctgcaaaaaa cagggacaat ccttcaagtt tgtatatgcc
     1381 attaaaagca accatattcc ctatcaaaat gcaggaaaat gccaagagaa aagacaaaag
     1441 catcttttta gagaaaacag ttcttctaac attcattagt aaaaatttca tttttacctc
     1501 ctagtaaaaa gagctaggtg aataaattcc actagctcaa tagtctcaat aaacaacttc
     1561 ctagctcatt actaatcact gttcttcatc tggactccaa ctgccagtcg tttgtactct
     1621 aactgtattt ccgaattgag tagctaatcc caaagtagtc gtttcattga cataatgact
     1681 accacttcca tgataagaca tgagaaccct agcaccagat ggaagagaat aggtcggagt
     1741 tatatcacta ccgctgtggt aatttcgtat tgaggtattt aaagtctttc caccacctgt
     1801 agaatcattg cgattcactc ctcgcgaatg agtcaccttg actatgctac cggttgttgt
     1861 agatcctata cgtggtaaga ccatggaata atctagtact cctcttgaaa aaaccgtcgg
     1921 agaaactaat cccaataatg ctattgctac gataattggc ttttttatag ataatctcgt
     1981 tttcatgtga atcttctcct ttcatgaaaa ccttataagg aagttgttta tattgatatg
     2041 taaattatat caagtatggt aaaa
//
LOCUS       DAZQCJ010000165         1960 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000165 DAZQCJ010000000
VERSION     DAZQCJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.167"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..431)
                     /gene="mreD"
                     /locus_tag="ACHU66_002077"
     CDS             complement(<1..431)
                     /gene="mreD"
                     /locus_tag="ACHU66_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR3390327.1"
                     /translation="MRNKMIEIFMFPILFFILLLDGQISTLVTNWSVGLFTISSHLVL
                     MLAIFYANYVSLGFSLFIFTLLGLVYDISYLNLIGIATTTLPLVLYCIYFFFQGAVSK
                     RGINILILLVAIFQFEFISYSFARIFHITNLSVFIFVFNKLL"
     gene            complement(421..1257)
                     /gene="mreC"
                     /locus_tag="ACHU66_002078"
     CDS             complement(421..1257)
                     /gene="mreC"
                     /locus_tag="ACHU66_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR3390328.1"
                     /translation="MNKFSKLVVVVSIFLLLSFSLLFVTFSKGLQVPYLNNIVRVVVT
                     PIQSVISVPTRFFSEQKDVLTDLMNAYEENKQLKEAIMSLEGMAAENTSLKEENASLR
                     SSLGVVSDFPEKQLIPGSVLVRTPSSWSEHISINIGETRGVTSNALVVANGGLVGIVS
                     SLSSDSAVVTLFTNSDEFTKLPVKISVDSKEIYGILSGYDADTNSFIINQLNSADEIA
                     VGSNVVTSDLAGATPANVQIGKVLSVKSNSNSLNREVYVEPTASFSNIYSVLVVGQTN
                     AQ"
     gene            complement(1317..1404)
                     /locus_tag="ACHU66_002079"
     tRNA            complement(1317..1404)
                     /locus_tag="ACHU66_002079"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1368..1370),aa:Ser,seq:gct)
     gene            complement(1417..1490)
                     /locus_tag="ACHU66_002080"
     tRNA            complement(1417..1490)
                     /locus_tag="ACHU66_002080"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1454..1456),aa:Ile,seq:gat)
     gene            complement(1522..1594)
                     /locus_tag="ACHU66_002081"
     tRNA            complement(1522..1594)
                     /locus_tag="ACHU66_002081"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1559..1561),aa:Phe,seq:gaa)
     gene            complement(1610..1683)
                     /locus_tag="ACHU66_002082"
     tRNA            complement(1610..1683)
                     /locus_tag="ACHU66_002082"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1647..1649),aa:Met,seq:cat)
     gene            complement(1694..1783)
                     /locus_tag="ACHU66_002083"
     tRNA            complement(1694..1783)
                     /locus_tag="ACHU66_002083"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1745..1747),aa:Ser,seq:tga)
     gene            complement(1790..1863)
                     /locus_tag="ACHU66_002084"
     tRNA            complement(1790..1863)
                     /locus_tag="ACHU66_002084"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1827..1829),aa:Ile,seq:cat)
     gene            complement(1880..1953)
                     /locus_tag="ACHU66_002085"
     tRNA            complement(1880..1953)
                     /locus_tag="ACHU66_002085"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1917..1919),aa:Met,seq:cat)
BASE COUNT          699 a          434 c          330 g          497 t
ORIGIN      
        1 agtaatttat taaaaacaaa gataaataca gacaagtttg ttatatgaaa aatacgagca
       61 aatgaataac taataaattc aaattgaaaa atagctacta gtaaaatcaa aatattaatt
      121 cctcgcttgc tgacagcacc ttgaaagaag aaatagatgc aatataaaac taaaggaaga
      181 gttgtagtag caataccaat cagattaaga taactaatat catataccaa acctagcaaa
      241 gtaaatatga ataatgaaaa accaagagat acatagttag cataaaaaat agctaacata
      301 agtaccaaat gacttgaaat agtaaataat ccgaccgacc aatttgttac caacgttgaa
      361 atctgtccat caagtaataa aataaagaac aagatgggaa acatgaatat ttctatcatt
      421 ttattgcgca tttgtttgac ctaccactaa aaccgaataa atgtttgaaa aactagctgt
      481 cggctcaaca tatacttctc tatttaaact gttactattt gatttaaccg ataaaacttt
      541 accaatttgg acatttgccg gagtcgcacc agccaaatca ctcgttacaa cattgctacc
      601 cactgcaatt tcatcagctg agttcaactg attaatgata aaactatttg tatctgcatc
      661 atagcccgat aaaataccat aaatttcttt agaatcaaca gaaattttca caggcaactt
      721 cgtgaattca tccgaattcg taaataaggt aacaaccgcc gaatctgaac tcaatgaact
      781 aacgatgcca actaatccac cattagcaac cacaagtgca ttagatgtca cacccctagt
      841 ctcaccaata ttaattgaaa tatgctccga ccacgatgaa ggagtcctca ctaaaactga
      901 tcctggaata agttgttttt caggaaaatc agataccaca cctaaactgc tacgaagcga
      961 tgcattctct tctttcaagc tagtattttc agcagccatc ccttcaaggc tcataatagc
     1021 ttcctttaat tgtttatttt cctcgtaagc gttcatcaaa tctgtcaaga catctttctg
     1081 ctcagaaaaa aatctagtag gtactgaaat aaccgattga attggcgtta caacaaccct
     1141 aacgatatta tttaaatatg gtacctgtaa tcccttagag aaagtcacaa acaaaagaga
     1201 aaatgacagt aataaaaaga tagaaacaac tactactaat tttgaaaatt tattcataat
     1261 ggatcctcac taagatacaa taaaacgaga atatcaagcg ataatctcgt tcactacgga
     1321 gaataaggga ttcgaaccct tgcgccagtt acccgaccta acgatttagc aaaccgtcct
     1381 cttcagcctc ttgagtaatt ctccatcaat ataatatggg cacgagtgga atcgaaccac
     1441 cgacctcacg cttatcaggc gtgcgctcta accatctgag ctacgcgccc aagataataa
     1501 acttggacaa agttaaatta atggcgcgag acggaatcga accgccgaca catggagctt
     1561 caatccattg ctctaccaac tgagctaccg agccaaattc ttaaagaaat tgcgggagca
     1621 ggatttgaac ctacgacctt cgggttatga gcccgacgag ctacctagct gctccatccc
     1681 gcggtagtag aaaaggagga tgtgggattc gaacccacgc acgcttttac acgcctgacg
     1741 gttttcaaga ccgttccctt cagccggact tgggtaatcc tccaatatat ggaccttgta
     1801 ggacttgaac ctacgaccgc tcggttatga gccgagagct ctaaccagct gagctaaagg
     1861 tccagcaaac aagagtatat agcggcgaag gggatcgaac ccccgacctc ccgggtatga
     1921 accggacgct ctagccagct gagctacacc gccaaaataa
//
LOCUS       DAZQCJ010000166         1942 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000166 DAZQCJ010000000
VERSION     DAZQCJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.168"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            141..1685
                     /locus_tag="ACHU66_002086"
     CDS             141..1685
                     /locus_tag="ACHU66_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR3390329.1"
                     /translation="MSLQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGNFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLDLLQELEEVLG
                     IASYPMNWPIGMGKSFEGLYDLHNKRLELYRGDERFASLDEGDKLFGSNPFYAQVLDD
                     IELLAEAGNEFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTTGD
                     VIDPLNKDFSGFVFKIQANMDPRHRDRIAFVRVVSGEFERGMAVNLPRTGKGAKLSNV
                     TQFMAESRENVENAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
BASE COUNT          548 a          419 c          483 g          492 t
ORIGIN      
        1 atagtttgaa aaattccctt atttccgata taataagaga gttgtctagt aaagaataag
       61 gatgaatcca gtgtaaatcg aataggataa gagctcagct ctgctatgaa tcaaaataca
      121 aagaatgaaa aggaaacatt atgtcactac aagaagaaat caagaaacgc cgcacctttg
      181 cgattatctc tcacccggac gcggggaaga ccactattac tgagcagttg ctctactttg
      241 gtggtgaaat tcgtgaagca ggtacggtca aggggaaaaa gactggtaac tttgccaaat
      301 ctgactggat ggatattgag aagcaacgtg gtatctctgt tacctcatct gtcatgcagt
      361 ttgactatgc aggcaagcgg gtcaatatcc tagatacacc agggcacgag gacttctcag
      421 aagacactta ccgtaccttg atggcggtgg atgcggctgt catggtggtg gactctgcca
      481 agggtatcga ggcccagacc aaaaagctct ttgaggttgt taagcaccgc aacattcctg
      541 ttttcacttt tatcaacaag ttggaccgtg acggtcgtga gccacttgat ttattgcagg
      601 agttggaaga agtcttgggc attgccagct atccaatgaa ctggccgatt ggtatgggga
      661 aatccttcga gggactctat gacctccata acaaacgttt agaactctat cgaggtgatg
      721 agcgttttgc cagcttggac gaaggcgaca agctctttgg ttctaatccc ttttatgcac
      781 aggttttgga tgatattgaa cttctggcgg aagctgggaa tgaattttca gaacaggcga
      841 ttttggacgg cgatttgaca cctgttttct tcggctcagc cctgacaaac tttggtgtgc
      901 agaccttcct tgacaccttc ttggaatttg ctccagagcc acatggtcac aagacaacga
      961 caggggatgt cattgacccg ctcaataaag atttttcagg ctttgtcttc aaaatccaag
     1021 ccaacatgga ccctcgtcac cgcgaccgta tcgcctttgt ccgtgtggtt tcgggcgaat
     1081 ttgaacgcgg tatggcggtt aacctgcctc gtacaggtaa gggagccaag ttgtccaacg
     1141 tgacgcagtt catggccgag tcccgtgaga atgtggaaaa tgcagtagcg ggtgacatta
     1201 tcggggttta tgatacaggg acctatcagg taggagatac cttgaccgtc ggaaagaaca
     1261 aatttgagtt cgaaccgcta ccgaccttca cccctgaaat tttcatgaaa gtttctgcta
     1321 aaaacgtcat gaaacaaaaa tccttccaca agggaatcga gcaactggta caggaagggg
     1381 ctatccagct ctataagaac taccagactg gcgagtatat gctgggtgcc gttggtcagc
     1441 tccagtttga agttttcaaa caccgcatgg aaggcgagta caatgccgaa gtagtcatga
     1501 ctccaatggg taaaaagaca gtccgctgga tcaagccaga agacctggat gaacgcatgt
     1561 catcaagccg caacatccta gccaaagacc gctttgacca accagttttc ctttttgaaa
     1621 acgacttcgc cctccgctgg ttcgcggaca aatatccgga tgtggagttg gaagagaaga
     1681 tgtagcggaa agacagaaat cagtaatcaa caaagttgat ttgatttcgt cgtctttccc
     1741 tcgcaagggc gtccaaagaa ctcgagcaat gcgaagagtg atttgctgac gcctactgcg
     1801 tcgaggttca gtgaaccttt tctagtggga gatgtgaact gacggtagtc agttcacatt
     1861 gataaagata actataaaag gatatgatag aatacatgaa gctgggcaag acctctatga
     1921 tgtcttgccc agcttcattt tt
//
LOCUS       DAZQCJ010000167         1924 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000167 DAZQCJ010000000
VERSION     DAZQCJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.169"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            133..372
                     /locus_tag="ACHU66_002087"
     CDS             133..372
                     /locus_tag="ACHU66_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFR3390330.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVQYLKILYNRN"
     gene            438..518
                     /locus_tag="ACHU66_002088"
     CDS             438..518
                     /locus_tag="ACHU66_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFR3390331.1"
                     /translation="MRYQMRYVDKTSTVLKQTKNSDYADK"
     gene            643..1380
                     /gene="erm(B)"
                     /locus_tag="ACHU66_002089"
     CDS             643..1380
                     /gene="erm(B)"
                     /locus_tag="ACHU66_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFR3390332.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1550..1924
                     /locus_tag="ACHU66_002090"
                     /pseudo
     CDS             1550..1924
                     /locus_tag="ACHU66_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          751 a          292 c          331 g          550 t
ORIGIN      
        1 tttctcaaaa taaacataaa agccctttaa agagggcttt tatatattaa tcacaaatca
       61 cttatcacaa atcacaagtg atttgtgatt gttgatgata aaataagaat aagaagaaat
      121 agaaagaagt gagtgattgt gggaaattta ggcgcacaaa aagaaaaacg aaatgataca
      181 ccaatcagtg caaaaaaaga tataatggga gataagacgg ttcgtgttcg tgctgacttg
      241 caccatatca taaaaatcga aacagcaaag aatggcggaa acgtaaaaga agttatggaa
      301 ataagactta gaagcaaact taagagtgtg ttgatagtgc agtatcttaa aattttgtat
      361 aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc
      421 atgttggtat tccaaatatg cgttatcaaa tgcgttatgt agataaaaca tctactgttt
      481 tgaaacagac taaaaacagt gattacgcag ataaataaat acgttagatt aattcctacc
      541 agtgactaat cttatgactt tttaaacaga taactaaaat tacaaacaaa tcgtttaact
      601 tctgtatttg tttatagatg taatcacttc aggagtgatt aaatgaacaa aaatataaaa
      661 tattctcaaa actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat
      721 ttaaaagaaa ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa
      781 ctggctaaaa taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta
      841 tcgtcagaaa aattaaaact gaatactcgt gtcactttaa ttcaccaaga tattctacag
      901 tttcaattcc ctaacaaaca gaggtataaa attgttggga atattcctta ccatttaagc
      961 acacaaatta ttaaaaaagt ggtttttgaa agccgtgcgt ctgacatcta tctgattgtt
     1021 gaagaaggat tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac
     1081 actcaagtct cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca
     1141 aaagtaaaca gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat
     1201 tggaagctat atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt
     1261 actaaaaatc agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccgtt
     1321 acttatgagc aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa
     1381 ttctatgagt cgcttttgta aatttggaaa gttacacgtt actaaaggga atgtagataa
     1441 attattaggt atactactga cagcttccaa ggagctaaag aggtccctag cgcctacggg
     1501 gaatttgtat cgataagggg tacaaattcc cactaagcgc tcgggacccc ttgtaggaaa
     1561 atgtcctaag tgtggcaaca atattgtatt aaaaaaatcg ttttatggtt gttcaaatta
     1621 tcctgaatgt accttcactt tagctgaaca ttttagaaag aaaaaactca ccaaaacaaa
     1681 tgtaaaagaa ttactagagg gaaaagaaac cctggtaaaa ggaatcaaaa cgaaagatag
     1741 aaagtcctac aatgccgttg taaaaatcgg agaaaaggga tatattagtt ttcttttgtt
     1801 agcagaagct atcttaaaaa atgaaataga aatagttgta gtaactgatc ataatactac
     1861 caaaggtatt aaaaaacttc aaacagcagt ttcaattata acgagaacct atccgactta
     1921 taat
//
LOCUS       DAZQCJ010000168         1905 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000168 DAZQCJ010000000
VERSION     DAZQCJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.170"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            291..1115
                     /locus_tag="ACHU66_002091"
     CDS             291..1115
                     /locus_tag="ACHU66_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3390333.1"
                     /translation="MITVQHIHKQFGDKVALDNISFDIQEGQIFGFLGPSGSGKTTMI
                     KIITGQLPADSGKSYLLGKESTLLSPEDFEAMGLVSDSSGFFEKMTLENNLLFYAKFF
                     DVSPKEVEVILQKVGLLDSKKTIAEKLSTGMKQRMLLARALINSPKVLFLDEPTSGLD
                     PTTSKRIHELLLELKGKGTTIFLTTHDMAEATFLCDQLVLLNQGNLVEKGSPQDLIHK
                     YNVTKKVRVTFEDGSDTELLFSELTPEKLAQATTIHSCEPTLEDIFIQQTGATLNV"
     gene            1108..1842
                     /locus_tag="ACHU66_002092"
     CDS             1108..1842
                     /locus_tag="ACHU66_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR3390334.1"
                     /translation="MYKRFLALLWLRTQVLVTNKNILLPVLMPYFMLLLFKFVMKTDD
                     TKAMDLMGICFSMAIGMSVGSPISAMISEEKEKNNLSTLLLTGVRPSEYVLSILCYPV
                     VISFVNLILFPAITQADVSGYMLSYSVIMLLTSLASILINLLIGATSQSQSKSQVTSM
                     MITMAVSMGPSLSLMNETVGQILKYSFLGAYIEFFKNPHFELLNQEFYYLLGWIVLIS
                     LALILYLGNWMKYFKNSLSGIKEKIQ"
BASE COUNT          608 a          318 c          397 g          582 t
ORIGIN      
        1 gccttgccta actctagtta tgcctgcggc tcgttgccta gtactaaaag taaactaaaa
       61 gactataaaa aattttttca aaaaatgcag acgatttgcg aataagtaag tgagagggaa
      121 aattcccctc aaggagatac acacttgaac aaaataaatc attttcaaaa tgatttacga
      181 ataaacaagt gtaggaaaaa tgaaaggaga agaaggatga ttatagcacc agctttatgg
      241 attttatcat ggtggaaaaa ataagtttgt tttaatttag gggagtagag atgataacag
      301 ttcaacatat ccataaacag tttggagaca aggttgcttt agacaacatt agctttgata
      361 ttcaagaagg acaaatcttt ggcttcttgg gtccctctgg atcgggaaag acaaccatga
      421 ttaagattat taccggccag ctaccagcag atagcggaaa atcctatcta ctaggtaaag
      481 aatcgaccct attgtcacca gaggactttg aagcaatggg cttggtcagt gattcgagtg
      541 gtttcttcga gaagatgacc ttggaaaata atttgttgtt ctatgcaaaa ttctttgatg
      601 tcagtccgaa agaagtagag gttattctgc aaaaagtagg gttactggat agtaaaaaga
      661 caattgcaga aaaactatct acaggtatga aacagcgaat gttgcttgcc agagccctga
      721 tcaattcacc aaaagtattg tttcttgatg agccgacaag tggtctagat ccaacaactt
      781 caaaacggat tcatgaacta ttattggagc taaaaggtaa ggggacgacg atcttcttga
      841 ccacgcatga tatggcagaa gcgacattct tatgtgatca attggttcta ttgaatcaag
      901 gaaatttggt tgaaaaggga agtccgcagg atttaattca taagtacaat gtcactaaaa
      961 aagtgagggt tacttttgag gatggtagtg atactgagct gttgtttagt gagttgacac
     1021 ctgagaagtt agctcaggca accactattc actcttgtga gccgactttg gaagacattt
     1081 ttattcaaca gacaggagca accttaaatg tataaacgat ttttagcctt gctttggctt
     1141 cgcacacaag tcttggtgac caataaaaac atcctattgc cagtattgat gccttacttc
     1201 atgttattgc tgtttaagtt tgtcatgaaa acagatgata ccaaggcaat ggatttgatg
     1261 ggaatttgtt tttcaatggc tattggtatg tcagtaggaa gtcctatatc tgcaatgatt
     1321 tctgaagaaa aggaaaagaa taatctgtct accttgttac tgacaggtgt tcgcccaagt
     1381 gaatatgtgc tgtctatttt gtgttatcct gttgtcatta gctttgtcaa cctcattcta
     1441 tttccagcta tcacacaggc cgatgtatca ggatacatgc ttagctactc agtcatcatg
     1501 ctcttgacat cattggcttc tattctaatc aaccttttaa tcggggcaac ttcccaatct
     1561 caatccaagt cacaggtgac ctcgatgatg atcacaatgg cagtatctat gggacctagc
     1621 ctgtctttga tgaatgagac agtggggcag attttgaaat atagttttct aggagcttat
     1681 attgaatttt tcaaaaatcc ccattttgaa cttttgaatc aagaattcta ttacctattg
     1741 gggtggattg tcctgattag tttggccttg attctctact tgggaaattg gatgaaatat
     1801 tttaagaact ccctatcagg aataaaagaa aaaatccaat agtctttata atatgagtaa
     1861 attagaaagg agaaatttat ggtatcagca ttcattatcg gtgtc
//
LOCUS       DAZQCJ010000169         1848 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000169 DAZQCJ010000000
VERSION     DAZQCJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.171"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..365)
                     /locus_tag="ACHU66_002093"
     CDS             complement(<1..365)
                     /locus_tag="ACHU66_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390335.1"
                     /translation="MLDKLRKSQNEYLELLRRVRGDLDLGDVAYHLDKIRIFWFRKQK
                     LLEVCSQHLFNNLDTYFYTAVSKFNVDNLDKNILFALGSYQIFDDPILSYLEVVEKSN
                     ALHDMKDYITKLKERIIEC"
     gene            complement(359..>1848)
                     /locus_tag="ACHU66_002094"
     CDS             complement(359..>1848)
                     /locus_tag="ACHU66_002094"
                     /inference="COORDINATES: protein motif:HMM:NF039499.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HFR3390336.1"
                     /translation="HCIVRYLMFSKLSDHDFKKGVFRTKFSQIVTPLDKESDWIHSKG
                     PEYLWIGLALQYGSRTEQMERMMLALTNLSKELDLEKILPLPAMSLILSLDIEEKKIF
                     VESLNSAFDLSIFSPLSIVLPDGEEVLSKNFYNRSHSFDQRLDVLVKVLDEISDQYSQ
                     LSTDVRYFLLYYKMLQGKIKFVESQSHMVDGLTKYPYLDISDPEMKIIRSQIRSMEVA
                     LSMSEGMNYLYSKRFWNNISQLTDCEVYSIVMDRSNAIDLNEIKGTVYSALKYYRDML
                     QNIEPFNEKLYVLTSILTYSYKRLLELMNHDLQYTISGRSIVRSCIENYVMTKYLIAE
                     EANHKNIWEEYQYYGIGGYKLVSERFIENQPELTNPHINFEYLNLLVSEFQNKEFIDM
                     DTRYFGNGNIRQKFKQVGEDDLYKFYYDYDSQFEHGLWGAIRESSVLKCTSAGHQFHG
                     IPDIDDVQELPDISHDMVMILTKHLSVINEVFPIPSERRDGDRLC"
BASE COUNT          610 a          343 c          226 g          669 t
ORIGIN      
        1 atgcactcta taatcctttc ttttaatttt gtgatgtaat ctttcatatc atgcaatgca
       61 ttactttttt ctaccacctc caagtaggat agtattggat catcaaatat ttgataactt
      121 cccaatgcaa aaagaatgtt cttatctaag ttatcaacgt taaattttga tactgcagtg
      181 taaaaatatg tatccaagtt attaaaaagg tgttgactac acacttctag taatttttgt
      241 tttctaaacc aaaaaattct aattttatct aaatgataag ctacatctcc taagtctaag
      301 tcgcctctaa ctctcctcag caactctaaa tactcatttt gtgatttcct aagtttatct
      361 agcataatct atcaccatct cttcgctcac ttggaatagg aaatacttcg ttaatgacag
      421 ataaatgctt tgttaatatc attaccatgt catgactaat gtcaggtaat tcctgtacat
      481 catctatatc aggaattcca tgaaattgat gcccagctga tgtacatttc aaaacagaac
      541 tctctctaat ggctccccaa agtccatgtt caaattgaga atcataatca tagtaaaatt
      601 tatataaatc gtcttcccct acttgcttaa atttttgtct tatattgcca tttccaaaat
      661 atctagtgtc catatctata aattctttat tttgaaattc cgacactagc aaatttaaat
      721 attcaaaatt aatatgagga tttgttaact ctggttgatt ctctataaag cgttcggaaa
      781 ctaatttata accaccaata ccataatatt ggtattcctc ccaaatgttt ttatggtttg
      841 cttcctcagc aattaaatat tttgtcataa catagttctc aatgcatgac cttactatag
      901 aacgccctga aatcgtatat tgcaaatcat gattcatcaa ctctagtaac cgcttataag
      961 aataggttag gatactagtt aaaacataaa gcttctcgtt aaaaggctct atattttgaa
     1021 gcatgtctct gtaatatttt aatgctgaat atactgtacc ttttatctca ttcaaatcta
     1081 tagcattact tctatccata acaattgaat atacctcaca atcagttagt tgactaatat
     1141 tattccaaaa tcttttacta taaagataat tcataccttc agacattgat aaagcaacct
     1201 ccattgagcg aatctgggaa cgaataattt tcatttctgg atctgatata tctaagtaag
     1261 ggtatttagt taatccgtct accatgtgtg actgcgattc tacaaattta atttttcctt
     1321 gtagcatctt gtaatacagt aaaaaatatc ttacatccgt tgacaactga ctatattggt
     1381 cagatatttc atctaatact ttaacaagta catctagtct ttgatcaaaa gaatgactcc
     1441 tgttgtaaaa atttttactc agaacttctt cgccatccgg caagactatt gaaagtggag
     1501 aaaatattga taagtcaaaa gctgaattta atgattcaac aaaaattttc ttctcttcaa
     1561 tatctaaact aagtattaaa ctcattgcag gtaaaggcaa gattttttct aaatctaatt
     1621 ctttagataa attagtcaga gctaacatca ttctttccat ttgctctgtt cggcttccgt
     1681 attgcagagc taatccaatc caaagatatt ctggaccttt tgaatgtatc caatcagatt
     1741 ctttatctaa tggagttact atttgactga atttagttct aaagacccct tttttaaagt
     1801 catgatcact taacttacta aacataagat acctcacaat acaatgtt
//
LOCUS       DAZQCJ010000170         1844 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000170 DAZQCJ010000000
VERSION     DAZQCJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.172"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            54..347
                     /locus_tag="ACHU66_002095"
     CDS             54..347
                     /locus_tag="ACHU66_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HFR3390337.1"
                     /translation="MQKTLEKLKRSELFELLVEQAKEIESYQAKLVDLEKRIEYREAV
                     LGEIGSIAEASLAVTRIFEEAQLAANIYLEQVQKVTHKQSDNCEIGRTDEDGD"
     gene            337..939
                     /gene="lepB"
                     /locus_tag="ACHU66_002096"
     CDS             337..939
                     /gene="lepB"
                     /locus_tag="ACHU66_002096"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR3390338.1"
                     /translation="MATNKNKDYVTISDLPSISIMSEELDKIRYKKRYLETLKNTIFT
                     LVTVVALAVLIAMLWLPVLHIYGSSMSPTLKAGDMVATVKTNTLSTGDMVAFYYNNKV
                     LVKRVVATSGQWVNIDEQGNVYANGKTLDEPYVKDKAYGQTDIKLPYQVPEGQYFVMG
                     DHRSVSIDSRNTAIGSIGEEQLVGKLTFRIWPFHKMGVIK"
     gene            958..1344
                     /locus_tag="ACHU66_002097"
     CDS             958..1344
                     /locus_tag="ACHU66_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390339.1"
                     /translation="MKILWERLFKFVQQCFREKKTAKITMALSIIMAFGISYLLILPA
                     ITLETDSQESVLQSVYNQPTTVEQPVQEEQAESQLAQVQSAGSLFAEHENITVQVNYE
                     EHTFEQPVRLQVLPSEDSTKYIGLVR"
     gene            complement(1345..1734)
                     /locus_tag="ACHU66_002098"
                     /pseudo
     CDS             complement(1345..1734)
                     /locus_tag="ACHU66_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1702..>1844
                     /locus_tag="ACHU66_002099"
     CDS             1702..>1844
                     /locus_tag="ACHU66_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFR3390340.1"
                     /translation="MASQIRHDLSLLVFFLITGLLLLLYPTVSDYWNSHYQSEAVATY
                     SEK"
BASE COUNT          640 a          282 c          386 g          536 t
ORIGIN      
        1 ttttgtagcg aaagcaatat aagcaaaaag gagagaggaa gaagcaatgt taaatgcaaa
       61 agaccttaga aaaactgaag cgttctgaat tatttgaact cttggttgag caggcaaagg
      121 aaatagagtc ttatcaagca aaattagtag atttggaaaa aagaattgag tatcgcgaag
      181 cggttttggg agaaattggt tcgatagcgg aggcgtctct agcagttact aggatttttg
      241 aagaagcaca attggctgca aatatttatc ttgaacaagt tcaaaaagtt acacataaac
      301 aatctgataa ttgtgaaata gggagaacag atgaggatgg cgactaataa aaataaggat
      361 tatgttacca tatcagattt acccagtata tcaataatgt cagaggagct ggataaaata
      421 cgatataaaa agcggtattt agagacatta aaaaatacta ttttcacgct tgttactgtc
      481 gttgcacttg cagtattaat agccatgtta tggttgccag ttctacatat ttatggaagt
      541 tccatgtctc ctactctgaa agcaggtgat atggtggcaa ctgtgaagac aaatacactt
      601 tcaactggag atatggttgc attctactac aataataagg ttttggtaaa aagagtagta
      661 gcaacttctg gtcagtgggt aaatatagat gagcagggga atgtttatgc aaatggaaaa
      721 acattagacg aaccctatgt taaagataag gcctatggtc aaacggatat taaactaccg
      781 taccaggttc cagaaggtca atattttgta atgggagatc atcgcagtgt atctattgat
      841 tctagaaata cagctattgg gtcgattggt gaggagcaac tggttggaaa gttaaccttt
      901 cggatatggc catttcataa aatgggagta atcaaataaa ggaaggagat ataaaacatg
      961 aaaatattat gggaaagatt gtttaaattt gttcagcaat gctttcgaga gaaaaagact
     1021 gcaaaaataa ctatggcgtt gtcaataatt atggcttttg gaatatcata tttgctgatt
     1081 ttaccagcca ttactttaga gacagacagt caggagagtg ttctgcaatc agtctataat
     1141 cagccaacaa cagtagaaca gccagttcaa gaagaacagg cggagtcaca gttagcacag
     1201 gttcaatctg ctggaagctt attcgctgaa catgaaaata taactgtaca agttaactat
     1261 gaggagcata catttgaaca gccagttcgg ttacaggttt taccctcaga agatagtaca
     1321 aaatatattg gacttgttcg gtaactacat catctcatgg ttgatgatac cacaaatcaa
     1381 ctcagttctt aattcaaacc gttttctacg attacgatag ggctgtgcca tgattttaaa
     1441 ggttttaaat ttggcattaa agtgttcaac ttcaatacgg attttagcta tctctcgatt
     1501 caaccgtcta tcctcttcgg ttaagaggtg atgtttagaa tgcttagcag gaataaaggt
     1561 attttcgtga aatttcaaga tgcctaaata ccctaaatct acgaaagcaa gggtattttg
     1621 gggcaaacta tagccaatac tctccttaaa aagggtaaaa tcatgcgtat gaccatctga
     1681 aaaagccaat tgacaagcac gatggctagt caaatcaggc atgatttgag tctgctagtc
     1741 tttttcttaa taactggcct attattgtta ttatatccaa cagtaagtga ttattggaac
     1801 tcacattatc aatcagaagc agtagctact tattctgaga aatt
//
LOCUS       DAZQCJ010000171         1790 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000171 DAZQCJ010000000
VERSION     DAZQCJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.173"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..258)
                     /locus_tag="ACHU66_002100"
     CDS             complement(<1..258)
                     /locus_tag="ACHU66_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad protein"
                     /protein_id="HFR3390341.1"
                     /translation="MKKKTTVFALTGIILSCHLFLAGCQKTENTTINHQDVQSSNISK
                     VDENTIVVSEIKSNGYMRLYGEESYFFEGPIPYAAHFLKDTL"
     gene            646..1398
                     /locus_tag="ACHU66_002101"
     CDS             646..1398
                     /locus_tag="ACHU66_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390342.1"
                     /translation="MENTATQAFEELVAEQKKKRNRVLRIIITLFTAIIVFFACLALF
                     KYNSGNISGTWRNSQAEQDIVKTSIKELQNQLPGIDKTWIDATYTMTVVDKKAVFTAT
                     TSFDIPKIAKLTLDQAYKVMLEEAQKEASSLGISVEEYLTTALGADYEKQLKSFLPTQ
                     AEAEKEFHKYQDEYAKELGATYNQETGTISATFFEGEVDQLFHTVTITKINDAAIKQL
                     TTYDISVKDIISYSRKENTTIINGNEEFTLEK"
BASE COUNT          648 a          321 c          308 g          513 t
ORIGIN      
        1 aagggtatct tttaaaaagt gtgccgcata cgggatagga ccttcaaaaa agtatgactc
       61 ttctccatat agacgcatat agccattaga tttaatttca gacactacaa tcgtattttc
      121 atctactttt gagatattgc tactttgaac atcttgatga tttattgtag tattctctgt
      181 tttttggcaa cccgccaaaa aaagatgaca agataggata atgcctgtca aggcaaatac
      241 ggttgttttc tttttcattt ttgtcctcct aaacttatta accagttaac aattaactag
      301 tatacatttt atgtttgatt tttgcaagaa aaaaacaact gacaatgatt gttcgtgaat
      361 aaattttcca ttccatggca aacgcatgaa aaatttgttt tataaaccat attttaacga
      421 ctgctgtcag tttttagtag aaaaaatgta atataatagc cttggaaatg ggggggatcc
      481 ttgttaaagt gcaagtttta caaaaacctt taaaaaaatg catgcaacaa gaaaatagtt
      541 acatgccttt ttcttcatac agatagcgtg tttccatccc tacgaccatt gacaaagcaa
      601 acaaactata gtatattaaa aatagggaaa taaaaggaga atattatgga aaacacagct
      661 actcaagctt tcgaagaact cgttgcggaa cagaaaaaga agcgtaatcg cgtccttaga
      721 attattatca cactctttac cgctattatc gtattttttg cctgtcttgc attattcaaa
      781 tataatagtg gcaacattag cggtacatgg cgcaattccc aagctgaaca agacattgtc
      841 aaaaccagta tcaaggagtt acaaaatcaa cttccaggta tcgataaaac gtggattgat
      901 gcaacttata cgatgacagt cgtagataag aaagctgtct ttaccgctac aacaagcttt
      961 gatattccta aaatcgcaaa attaaccttg gatcaagcct ataaggtgat gttagaggaa
     1021 gcacaaaaag aagcaagttc tctgggaatt tccgtagaag aatatttaac aacagctctc
     1081 ggagcagact atgaaaagca attgaaatca tttctaccta cacaagctga ggcggaaaaa
     1141 gaatttcata agtaccaaga cgagtatgct aaagaactag gtgccacata caatcaagaa
     1201 actggtacta tctctgctac ctttttcgag ggagaagtcg atcagctctt ccataccgtg
     1261 accattacaa aaattaatga cgcagcaatc aaacagctga ctacatacga tatttccgta
     1321 aaagatatta tttcctattc tcgcaaggaa aataccacca ttattaacgg aaatgaagaa
     1381 tttactttag aaaaataata aaaaagctgg gaaagtcatc cctgatggtt ccgacctcac
     1441 ccacagaata atgaaaataa atatgaagcc ctcactaggt gtataatctt agtgagggct
     1501 ttgtgcttga cgagttaatg atgttagtca tcgtcggaaa aactaaccca ctctattgac
     1561 aagctctaaa aaatatgtaa cagacatgag cataagaact agccaataaa aacacagata
     1621 gattcaagct gattttgatg aaatacagcc gaattgtctg tgcttttatt tttgtctcta
     1681 ctatagatag gacaacattg tgacgatatt tgaaagaact tgtactaata ctcaatgaaa
     1741 atcaaaagta gcctaggaaa cgaagccgaa gatagaactg gagttcatca
//
LOCUS       DAZQCJ010000172         1758 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000172 DAZQCJ010000000
VERSION     DAZQCJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.174"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..585
                     /locus_tag="ACHU66_002102"
     CDS             <1..585
                     /locus_tag="ACHU66_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFR3390343.1"
                     /translation="YWFHHGHLGLTKADMLYPRKGKRVKKQASPNFKPAGKSIEERPD
                     VINLRLENGHYQIDTVLLTRGKNHCLLVLTDRRSRHQIIRLIPNKTAESVNQALEGIL
                     REHRILSITVDNGTEFSRLAEVLPEEYIYYAHPYSSWERGTNENHNRLIRRWLPKGTK
                     KTTPKEVAFIEHWINNYPKKCLDYKSPSEFLMGG"
     gene            complement(944..>1758)
                     /locus_tag="ACHU66_002103"
     CDS             complement(944..>1758)
                     /locus_tag="ACHU66_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR3390344.1"
                     /translation="YSFRTNDIRSYLTFLNQHIKKTSTLKRKIASLKSYYKFLEEEKL
                     ISKNPFHQLRLHYKEEKKLPKTISLKDLQKIYCFAHQQIENSKNKHSKRKAMRNLLLI
                     SLLLSTGLRISELCNLKLDNINLENRTIQILEKGKKVRLLYIGNDETLSLLEDYIAHH
                     CSHSSYLFTSVKNDDHLKEQSVRLSLKNISHSLQLKKHITPHMFRHSFATMLLDNGVD
                     IRQIQHLLGHSNINVTQIYTHVSQSKQVEILSEHNPLNQITPLLPANQVDLT"
BASE COUNT          544 a          293 c          353 g          568 t
ORIGIN      
        1 tattggtttc atcatggcca tttaggattg acgaaagcag atatgcttta ccctagaaaa
       61 gggaaaaggg tcaagaagca agctagtcct aactttaaac cagcaggaaa atctatcgaa
      121 gaacgtcctg acgttattaa tcttcgcttg gaaaacggtc attatcaaat tgatacagtt
      181 ctactaacca gagggaaaaa tcattgcctc ttagtcttaa ccgaccgacg aagcaggcac
      241 caaatcatac gattaattcc aaacaaaacc gccgaatcgg tcaatcaggc actggaggga
      301 atcttgaggg aacatcgtat cctgtctata actgtagata atggtacaga atttagtcgt
      361 ctggctgaag tgttacctga ggagtatatc tactatgcgc atccctactc ttcatgggag
      421 aggggaacga atgaaaatca caatcgattg attcggagat ggttacctaa aggaaccaag
      481 aaaacgactc caaaagaagt cgcttttatc gaacattgga tcaacaacta ccctaaaaaa
      541 tgcttggact acaagtcgcc gagtgaattt cttatgggtg gctaatttca acttgaaatt
      601 tggggtctaa gctacttata acatttgaaa ccttaattta taagtttgag ttgaggattg
      661 tggatttcaa gcagctgttt tcttctaaaa aaataacaaa atcgaaacgg tccatatcca
      721 ctttgagtgg tagttggcta tttttgattt taaatgagta tcacacccca tcgtcccatt
      781 gactgttccc caatctcgcc gctttgagaa gaaaggcaga aatgatattc tgatgaaaat
      841 tcgtctcgga aaagtggaag tcacattctt tcactctatc aaccaagaaa cattagaaac
      901 aatcttagat aaggtactat tttatgacca tccgactcag tgattaggtc aagtctactt
      961 ggtttgcggg aagtagtggt gtgatttggt taagaggatt atgctcactt agaatttcta
     1021 cctgcttgct ttgagagaca tgagtgtaga tttgtgttac attgatgtta ctgtggccca
     1081 gtaaatgttg tatttgtcga atatcgacgc cattatcaag caacatagtt gcaaaactat
     1141 gtcgaaacat atgtggagtg atatgttttt tcagttgtag cgagtgagaa atatttttca
     1201 gacttaagcg cacactttgt tctttaagat gatcatcgtt tttcacactg gtaaatagat
     1261 aactagaatg actacaatga tgcgctatat agtcttcaag taacgaaagt gtttcatcgt
     1321 ttccaatata gagtagtcgc actttctttc ctttttcgag tatttgaatg gttctatttt
     1381 ccaaattgat attatcaagt tttaaattac ataattcaga gatgcgtaga ccagttgata
     1441 aaagtagcga gataaggagt agatttcgca tagctttccg cttagaatgc ttatttttag
     1501 aattttctat ttgttggtgg gcgaaacaat atattttttg taaatctttc aaggaaatcg
     1561 ttttaggaag ctttttctct tccttgtagt ggagtcgaag ctgatggaag ggattttttg
     1621 aaattagttt ttcttcttcc agaaatttat agtatgattt aagactagca atcttacgtt
     1681 ttaatgtgct agtctttttt atatgttggt tgagaaaagt aagataggac ctaatatcat
     1741 tggtcctaaa gctataat
//
LOCUS       DAZQCJ010000173         1747 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000173 DAZQCJ010000000
VERSION     DAZQCJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.175"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..1729)
                     /locus_tag="ACHU66_002104"
     CDS             complement(<1..1729)
                     /locus_tag="ACHU66_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HFR3390345.1"
                     /translation="MKLTIFDKVQNKTSQFAIDELTYYLKAIFDFELGRTTVEEDANI
                     LLSKEYFDNNDTIGIEMKNGNAILSGNSDISILIAVYRLLSEFGAVFTRPGRKHELIP
                     SLSISDWNEKELSIHETASYKHRGVCIEGADSYQNIKEFLDWLPKINMNSFFIQFENP
                     YSFLKRWYEHEFNPYADKEDFNTEIAQQMSDLLDEELSKRGLIHHRVGHGWTGEVLGY
                     SSKYGWESGLILPEEKKPLVAELNGKRELFNTAPILTSLCFSNPDVGERMCELIVNYA
                     KERKDVDYLHVWLSDARNNICECEKCKEEIPSDQYVRILNQLDRKLSEEGLNTKICFL
                     LYHELLFAPKHEVLENPERFTMMFAPITRTFEMSYADVDYENNVPLPNEYTRNNIVLP
                     NSLEENLSYLFSWQKVFNGDSFVYDYPLGRAHYGDLGYMKISKVIFRDVVYLKKLGLN
                     GYISCQELRAGFPHNFPNFVMGQMLWNRNLEYDELKALYFSSLYGSEWKSVVTYLEKL
                     SDYSSCDYFNSIGDRQNAELSMKYNISSKLAFNFINILEENVAKNYGIKRDAWRQLGY
                     HREYVIKLAN"
BASE COUNT          560 a          343 c          240 g          604 t
ORIGIN      
        1 cgttggccaa tttgataaca tattctcggt gataacctaa ttgtcgccaa gcatcacgtt
       61 taattccgta atttttagcc acattctctt ccaagatatt tataaaatta aatgctaatt
      121 tacttgagat attgtatttc attgataatt cagcattttg gcgatcacct attgaattaa
      181 aataatcaca tgaagagtaa tctgataatt tttctaagta cgttacaaca cttttccatt
      241 cagaaccata taaggaactg aaatagagcg ctttcaattc atcgtactct aaatttctgt
      301 tccacaacat ttgacccatc acaaagttag ggaagttatg agggaaacct gcgcgtaact
      361 cttggcaact aatataacca tttaaaccca attttttaag atatacgaca tctctaaaga
      421 ttactttact gatcttcata tatcctaaat caccataatg tgcccgacca agtgggtaat
      481 catagacaaa actatctcca ttaaacactt tttgccaact aaataaatat gagagatttt
      541 cttcaagtga gttaggaaga acaatattat ttcttgtata ctcatttgga agtgggacat
      601 tgttttcata gtctacatca gcataactca tttcgaaagt tctggtaata ggcgcaaaca
      661 tcattgtgaa acgttcagga ttctctaaaa cttcatgctt tggtgcaaaa agcaattcat
      721 gataaagtaa aaaacaaatc tttgtattaa gcccctcttc acttaatttc cgatctaatt
      781 gattcaaaat tcgtacatac tgatctgagg gtatttcttc tttacatttt tcacattcac
      841 aaatattgtt ccgtgcatcg gataaccaaa catgtaagta gtctacatct ttcctttcct
      901 ttgcatagtt tacaattagt tcacacattc tttcaccaac atctggattt gagaaacata
      961 gactagttag tattggcgct gtattaaata actcacgttt tccatttaat tcagcgacta
     1021 ggggtttctt ttcttcaggt agtattaacc cagattccca tccgtactta gatgaatatc
     1081 caagtacttc accagtccaa ccatgtccaa cacgatgatg tattaaacct cgttttgata
     1141 attcttcatc taaaaggtcg ctcatttgtt gtgcaatttc cgtattgaaa tcttctttat
     1201 ccgcatacgg attaaattca tgttcatacc agcgctttaa aaatgaatac ggattctcaa
     1261 actgaataaa aaagctattc atattaattt ttggtaacca atccaaaaat tcttttatat
     1321 tctggtaact atctgcacct tctatacata ctcctcgatg tttataactg gcagtctcgt
     1381 gaatagagag ctctttctca ttccagtcag atatcgataa cgagggaatt agttcatgtt
     1441 ttctacctgg tctagtgaat acagctccaa attcactaag taagcgatat accgcaataa
     1501 gaatagagat atcagaatta cctgataata tcgcatttcc attcttcatc tcaatcccaa
     1561 tcgtatcatt attgtcaaaa tattcttttg agagtagaat atttgcatct tcctcaacag
     1621 ttgtccttcc taactcaaaa tcaaaaatgg ctttcaagta atatgtaagt tcatctatag
     1681 caaattgaga agttttattc tgtactttat caaaaatcgt taatttcata tgatcactct
     1741 cctgatg
//
LOCUS       DAZQCJ010000174         1729 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000174 DAZQCJ010000000
VERSION     DAZQCJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.176"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..563
                     /locus_tag="ACHU66_002105"
     CDS             <1..563
                     /locus_tag="ACHU66_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFR3390346.1"
                     /translation="SILNITDTGIMGYVEIPKLQTMLPIYHGTDDAVLQVAVGHLAGT
                     SLPIGGQGSHAAISGHRGLPSAKLFSDLDQLVIGDTFMIQVLDNTLTYEIDKIITVTP
                     DDVSPLMIDPNEDFVTLVTCTPYGVNSHRLLVRGHRIENQLLARQVVSEGSIVKPILV
                     SPFIALCLLGLLWSLFFVKKEVNLFM"
     gene            694..870
                     /locus_tag="ACHU66_002106"
                     /pseudo
     CDS             694..870
                     /locus_tag="ACHU66_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104139.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            970..1380
                     /gene="nmlR"
                     /locus_tag="ACHU66_002107"
     CDS             970..1380
                     /gene="nmlR"
                     /locus_tag="ACHU66_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stress response transcriptional regulator NmlR"
                     /protein_id="HFR3390347.1"
                     /translation="MNIKEVSDVTGLSADTIRYYERIGLIPKIARKSSGVRNFSESDV
                     AILEFIRCFRSAGMSIERLIEYMGLVQAGDSTVEARIDLLKEEREELRSRLSEIQQAL
                     DRLDYKIENYQTILRGAENQLFTDGGSSFKKGRG"
     gene            complement(1402..1716)
                     /locus_tag="ACHU66_002108"
     CDS             complement(1402..1716)
                     /locus_tag="ACHU66_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HFR3390348.1"
                     /translation="MALRHYQSHEYQYEEYIPKEKKANFQTYQAKNTNKERLKELLFF
                     VNIAFFSLITVIASYVYLSVGIPVFVAIILASATGFLGLRLVQIGLKKKFKPKQVIRK
                     NE"
BASE COUNT          531 a          297 c          363 g          538 t
ORIGIN      
        1 actcaatttt aaatatcaca gatactggta ttatgggcta tgtagaaatt cctaaattgc
       61 agacaatgtt acctatttat cacggaacgg atgatgccgt attgcaggta gcagtaggtc
      121 accttgctgg gacttccctt cccattggcg gtcagggaag tcacgctgct atttcaggac
      181 atcggggatt gccttctgca aaactgtttt ctgatttaga tcagctggtt ataggggaca
      241 catttatgat acaagtttta gataacactc tgacatacga aattgataaa attattacgg
      301 ttactccaga tgatgtatct cctttgatga tagatccgaa tgaggatttc gtaacccttg
      361 tgacttgcac accttatggt gttaatagcc accgattatt agttagaggt catcgaattg
      421 aaaatcaact acttgctagg caagtagtct cagaaggatc aattgtaaaa cctatactgg
      481 tttctccttt tattgcttta tgtttattag gactactttg gagtttattt tttgtaaaaa
      541 aagaagtaaa tttgtttatg tgacttagat agtatcgcaa agaatgtcaa actattcgac
      601 tttgttcttt agcttttaag cttaggaaag ggagattttt ccaactccca atcatccaaa
      661 aggtgaaata gaagataaac caaatgacga tatgtcaatg tcctctatac gctagaaaaa
      721 ggtggcaagc gagctgtcgg attcaaattg tcagacggca ttgacattcc taaggaattt
      781 gaaggtaaat ttaagtttgc ccgccaacgc tccaaactag ctggcgaaat tcgtggcacc
      841 ttctttgttg tcaagggcga gtatttatag gatggcaaat ctgaggagac tcagattttt
      901 taaataatga cttgacctgg agtggacttc aaggtgtata ataagcgaaa acaaagaaag
      961 gagcctggaa tgaatatcaa agaagttagt gatgtaacag gtctatcagc agacaccatt
     1021 cgctactatg agcggatagg acttattccc aaaattgcaa gaaaatcttc tggagtaagg
     1081 aacttttcgg aaagtgatgt agccattttg gaatttatcc gctgttttag gagtgctggt
     1141 atgtctattg agcgcttgat tgagtatatg ggcttggtgc aggcaggaga tagtacagtg
     1201 gaagctcgga ttgatttact aaaagaggaa cgggaagaac tgcggtcacg cttgtcggaa
     1261 attcagcaag ctctagatcg tttagattat aaaatagaaa attaccagac tatattaaga
     1321 ggtgcagaga atcaattgtt cacagacggc ggtagttcat tcaaaaaagg cagaggataa
     1381 cttctctgcc tttatacttc attattcatt ttttcttata acttgtttgg gtttaaattt
     1441 cttctttagc ccaatttgaa ccaaacgtag tccaagaaaa cccgtcgcac ttgccaatat
     1501 gatggcaaca aaaacaggaa tgcctacgga tagatagaca taactagcga tgactgttat
     1561 caaactaaaa aatgcgatat taacaaaaaa caacaattcc tttagacgtt ctttattggt
     1621 attttttgcc tgataggttt gaaaattagc ctttttctct ttcgggatat actcttcgta
     1681 ctggtactca tgggactgat agtgtcttaa tgccatatcg tctcctttt
//
LOCUS       DAZQCJ010000175         1691 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000175 DAZQCJ010000000
VERSION     DAZQCJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.177"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(114..1391)
                     /locus_tag="ACHU66_002109"
     CDS             complement(114..1391)
                     /locus_tag="ACHU66_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFR3390349.1"
                     /translation="MSTKANLLFDVHEKPAPLQGILLSFQHVFAMFGATILVPLILGM
                     PVSVALFASGVGTLIYQVATQFKVPVYLGSSFAYISAMALAIKEMGGDVSAAQTGILF
                     VGLIYVLIAALVKVIGTKWIDSLLPPIVIGPMIIVIGLGLANSAVTSAGFVADGDWKN
                     VVVAIATFLIAAFVNTKGKGFAKIVPFLIAIIGGYVIAIFLGLVNFTPVLEAAWFELP
                     GFYLPFETGAFKSYNFYFGPEMIAILPIAVVTVAEHIGDHTVLSQICGRQFLKDPGLS
                     RTLIGDGVATAVSAFIGGPANTTYGENTGVIGMTRIASVSVIRNAALIAIAFSFLGKF
                     TALISTIPSAVLGGMSILLYGVIASNGLKVLIESRVDFGQVRNLIIASSMLVLGLGGA
                     VLNIGAITLSGTALSAIVGIILNLILPKAEKAE"
     gene            complement(1506..>1691)
                     /locus_tag="ACHU66_002110"
     CDS             complement(1506..>1691)
                     /locus_tag="ACHU66_002110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390350.1"
                     /translation="TGRWLWHEKSSFKVSACGKRVGRRNGVIISSSASCSAIFLKNKN
                     LQNSLPKVRAYVTIPKA"
BASE COUNT          527 a          398 c          358 g          408 t
ORIGIN      
        1 gagaattaaa caactaaacc tttcacactc ctcgaaaact aattatttca ccccaatctc
       61 ctctacacca tcaaaaaaag ccagccgggg ctgacttgct gttattttca attttactca
      121 gccttttctg cttttggcaa gataagattg aggataatac ctacaatggc tgagagggct
      181 gtacctgaaa gggtgatggc accgatgttg agaactgctc caccaaggcc caataccagc
      241 attgagcttg caataatcag gttgcgaact tgaccaaagt caacacggct ttcaatcaaa
      301 actttcaaac cgtttgaggc gataacaccg taaagtaaga tagacatacc accaagaacg
      361 gcacttggaa tagttgagat aagggctgta aatttaccca agaatgagaa ggcaatggca
      421 atcaaggctg cattgcggat aacggatact gatgcgatgc gagtcatacc gataacccct
      481 gtattttcac cgtaggtcgt attggctggt ccaccgataa aggcagatac agcagtcgct
      541 acaccatctc caatcaaggt acggctgagg cctggatctt tcaagaactg gcggccgcag
      601 atttggctga gaacggtgtg gtcaccgata tgctcagcta ctgttacaac agcaattggt
      661 aagatagcaa tcatttctgg accgaagtag aaattgtacg acttgaaggc acctgtttca
      721 aatggtaggt agaaacctgg taattcaaac caagccgctt ccagaacggg tgtaaagtta
      781 accaggccaa ggaagatggc aatcacataa ccaccgataa tggcaatcaa gaaaggaaca
      841 atcttggcga agcctttccc tttggtattg acaaaagccg caatcaagaa ggtcgcaatc
      901 gccacgacga cgtttttcca gtcgccgtct gcgacgaagc ctgctgaagt cacggcagag
      961 ttggcaagac ccagaccgat gacgataatc attggaccga tgacaatcgg tggcaagagg
     1021 ctgtcaatcc acttagtacc gataactttg acaagtgcag cgatcaacac gtaaatcaag
     1081 ccgacgaaga gaataccagt ctgagctgcc gacacatcac cacccatttc cttgatagca
     1141 agcgccatag cagaaatgta ggcaaaggat gaacccaggt aaactggaac tttaaactgg
     1201 gttgccactt gataaatcaa ggtcccaaca cctgatgcga agagggcaac cgatacaggc
     1261 ataccgagaa tcagtggcac caaaatcgtc gcaccaaaca tagcgaacac gtgctggaaa
     1321 ctcaacaaaa tcccttgaag cggtgctggt ttctcatgga catcaaacaa aagattggct
     1381 tttgtactca taaatctccc attctgggta cacaaaaaga cccagacttt ctaaatatac
     1441 atagtcatag gcccttaatg aattatcctg ttgtctttct cacctcacgg gatgagttta
     1501 aaaacctacg cttttggtat tgtaacataa gcccttactt ttggcaagga attttgtaaa
     1561 tttttatttt ttaggaaaat ggcagagcag gaagctgaac tgcttattat tactccattt
     1621 cttcttccaa ctctttttcc acacgcgcta actttaaacg aagatttttc atgccatagc
     1681 catcttcctg t
//
LOCUS       DAZQCJ010000176         1680 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000176 DAZQCJ010000000
VERSION     DAZQCJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.178"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            74..1654
                     /locus_tag="ACHU66_002111"
     CDS             74..1654
                     /locus_tag="ACHU66_002111"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR3390351.1"
                     /translation="MKFTDLQLSEDILLAVEKAGFETPSPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIDTNNQAIQALIIAPTRELAVQSQEELFKFGREKGVKVRS
                     VYGGSSIEKQIKALRSGAHIVVGTPGRLLDLIKRKALKLDGVETLILDEADEMLNMGF
                     LDDIEAIIERVPESRQTLLFSATMPEPIKRIGVKFMKEPEHVKIAAKELTNVNVDQYY
                     LRVKEHEKFDTMTRLMDVDQPELSIVFGRTKRRVDELTRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDQIDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     QSGQSITFVSPNEMGYLAIIEDLTKKRMKGLKPATAQEAFEAKKKVALKKIEREMADE
                     TIRSNFDKFKKDAIQLAAEFTPEELALYVLTLTVQDPESLPEVEIAREKPLPFKFAPG
                     QAKGKGGRGGRDRDRGGRRDGDRNRDRGGRRGDRNDRNRRDDKFKRDNRRQDNKKPHQ
                     RTSSEKKTGFVIRNKGER"
BASE COUNT          491 a          360 c          414 g          415 t
ORIGIN      
        1 ttcgaccctg atatcttgtt acaaaaaagt tagataaggt agcttcgcac tgccttttag
       61 aaaagagaat acattgaaat ttacagattt acaattatca gaagatattt tgcttgccgt
      121 tgagaaggct ggttttgaaa cgccgtcacc aatccaggag cagaccattc cgcttgctct
      181 tgaaggtaaa gatgtgattg gtcaagccca gacggggaca gggaaaactg cagcctttgg
      241 tttgccaacc ctcaacaaaa ttgacacaaa caatcaagca attcaggcct tgattatcgc
      301 cccaacacgt gagttggcgg tgcagagtca agaagaactt ttcaaatttg gtcgtgaaaa
      361 gggtgtgaaa gtccgctctg tttacggtgg ctcaagcatt gaaaagcaaa tcaaggccct
      421 tcgttcaggt gctcacatcg ttgttggtac accaggtcgc ctcttggact tgatcaaacg
      481 caaggccctc aaacttgacg gtgttgaaac tctcattctt gacgaggcag atgaaatgct
      541 caacatgggc ttcttggatg atattgaagc gatcatcgaa cgtgtaccag agagccgtca
      601 aacccttctg ttttctgcaa ccatgccaga gccaatcaag cgtattggtg tcaagttcat
      661 gaaagaacct gagcacgtta agattgcagc aaaggaattg actaatgtca atgttgacca
      721 atattatctt cgtgtcaaag aacatgaaaa attcgacacc atgactcgtc ttatggatgt
      781 tgaccagcca gaattgtcta ttgtctttgg tcgtaccaaa cgccgtgtcg atgaattgac
      841 tcgtggtttg aaattgcgtg gtttccgtgc agaaggtatt cacggagatt tggaccagaa
      901 caagcgtctt cgtgtcattc gtgattttaa aaatgatcaa attgatgtct tagttgcgac
      961 agacgttgcg gcccgtggtt tggacatctc aggtgtaacg catgtctata actacgatat
     1021 tccacaggac cctgaaagct atgtgcaccg tatcggtcgt acaggtcgtg cgggtcaatc
     1081 aggtcagtct attacctttg tttcgccaaa cgagatgggc tacttggcca tcatcgaaga
     1141 tttgaccaag aaacgcatga aaggtctcaa gccagcaact gctcaggaag catttgaagc
     1201 caagaagaaa gttgctttga agaagattga gcgtgaaatg gcggatgaaa ccatccgtag
     1261 caattttgat aagttcaaga aagatgctat tcagttagcg gctgaattta cccctgaaga
     1321 gttagcgctt tatgtattga ccttgactgt tcaggatcca gaaagtctgc cagaggtaga
     1381 aattgcgcga gaaaaaccac tgccattcaa gtttgcacct ggtcaagcca agggcaaggg
     1441 tggacgtggt ggtcgcgacc gtgatcgcgg tggtcgtcgt gatggagacc gcaaccgaga
     1501 ccgcggagga cgccgtggtg atcgtaatga ccgcaaccgt cgcgatgaca agtttaaacg
     1561 cgacaaccgc cgtcaggaca acaaaaaacc acaccaacgc acatcaagtg aaaagaaaac
     1621 aggctttgtt atccgcaata aaggggagag ataagccgga tttgacagac cataaagagt
//
LOCUS       DAZQCJ010000177         1673 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000177 DAZQCJ010000000
VERSION     DAZQCJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.179"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..606)
                     /locus_tag="ACHU66_002112"
     CDS             complement(<1..606)
                     /locus_tag="ACHU66_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR3390352.1"
                     /translation="MKSRIVSPKTIFIWNLLGSISSAAISIFLLLLVTRLLTELEADI
                     FSFAYAVANLFVIIASFQVRDYQATDVSKKFSFSQYLATRLITITIMLLLALSYIFLS
                     KYEFQKSACIFLICLYRGSDALSDVFQGLFQQNARLDIAGKSLFLRNSIVILTFGFGL
                     FITNNLLLSLIYLVISSYLFVFFFDVTNLFQFTRIIKEEINL"
     gene            complement(639..>1673)
                     /locus_tag="ACHU66_002113"
     CDS             complement(639..>1673)
                     /locus_tag="ACHU66_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HFR3390353.1"
                     /translation="PDRIYSLLHLIRMKTKYPVELIERHLRQIIIPGTSFIPPIRVSQ
                     TTETVRSSTSVLLHVHIESVSIFEEYIEELCKISDRCQLLITLPETDFSNKCSIVERY
                     LSTYKLRAQIAKLTDELHFFEIVNNYMGDAKYLAHVTVKQTKEIKYSVEDIIDRHQLR
                     KMFFTSFDAVISNFESQSNLAVVIPDLTTNQRYDRQSLREGNPELIRQLNILYESLVR
                     TKKVDFYKVPYIIGEEVSWYWIKTEDYKKIEEKFRNIDFSKEDRLLLVPILFIYSAWD
                     LSNDYAVVENTENVSPILEKISFSSERELRLIIEEKEFLQIGLRRTLKIISVGIVSVF
                     KMIKKSLLKN"
BASE COUNT          579 a          307 c          217 g          570 t
ORIGIN      
        1 caaattaatt tcttctttga taatcctcgt aaattgaaac aaattcgtaa catcaaaaaa
       61 gaaaacaaat aaatatgagc taattactag atagattaat gagagtagca ggttattggt
      121 tataaacaaa ccaaatccaa atgtcaaaat cacaatagag tttctcaaaa aaagtgattt
      181 acctgcaata tctagccgag cattttgttg gaacaatcct tgaaaaacat ccgatagagc
      241 atcacttcca cgatacaaac atattaggaa aatacaagca gatttttgaa attcatattt
      301 agacagaaaa atatagctta aggcaagtaa tagcataata gtgattgtta taagccttgt
      361 tgcaagatac tggctaaacg aaaacttttt cgaaacatca gtagcttgat aatctcttac
      421 ctgaaaacta gcaatgatta caaaaagatt tgctactgca taagcaaaac taaaaatatc
      481 agcctctaat tccgtcaaca aacgtgttac tagtaacaat aaaaatattg atattgctgc
      541 tgaagaaata cttcctaata aattccaaat aaatattgtt tttggtgata ctattctgct
      601 cttcacacta tcttcccctt gtttgtcatc cgttatccct aattttttaa tagagatttc
      661 tttatcatct taaacacgct tacaattcca actgatatta tctttagtgt ccttctcagt
      721 ccaatttgta aaaattcctt ttcttcaata attaaacgca attctctttc gctagaaaaa
      781 ctaatctttt ccaatattgg actcacattt tcagtattct cgaccactgc ataatcgttt
      841 gataaatccc acgcgctata aataaataat attggaacta gcaataatct atcttctttt
      901 gaaaaatcaa tgtttctaaa tttctcctca atctttttat agtcttctgt ttttatccaa
      961 taccaactta cttcttctcc aattatataa ggcactttat aaaaatcaac tttcttagtt
     1021 ctcacaagtg attcataaag tatgttcaat tgacgaataa gttctggatt gccctctcga
     1081 agactttgtc tatcatatct ttgattagtt gttaagtctg gaataactac agccaaatta
     1141 gattgtgatt caaaattaga aatcactgca tcaaatgatg taaaaaacat tttcctcaac
     1201 tgatgcctgt caataatatc ctctacagaa tattttattt cctttgtttg tttgacagtt
     1261 acatgagcaa gatatttggc atctcccata tagttgttta ctatttcaaa aaaatgcaat
     1321 tcatctgtga gttttgcaat ttgggctcgc aatttgtaag tagacaaata tctctcaact
     1381 atactacact tatttgaaaa atcagtttct ggcaatgtta ttagtaactg acacctatca
     1441 gatattttgc ataactcttc aatatactct tcaaaaatag acactgattc tatgtgaaca
     1501 tgaagaagta ccgacgtcga agaacgaacg gtctccgtcg tttgcgaaac ccgtattgga
     1561 ggaataaaac tcgtacccgg aataattatc tgcctcaaat gcctctcgat taattcaact
     1621 ggatatttcg ttttcatcct tataagatgt aataaagagt atattctgtc tgg
//
LOCUS       DAZQCJ010000178         1600 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000178 DAZQCJ010000000
VERSION     DAZQCJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.180"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            121..879
                     /locus_tag="ACHU66_002114"
     CDS             121..879
                     /locus_tag="ACHU66_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR3390354.1"
                     /translation="MKSNKEQKYFWLTFSLIVALMIWWFLSNYDRTSRSFPNIILVWN
                     ALLIMIDKGFIFEDIFSSLTSVFFGFTSGFLLSVPNALFMSWYSPYRNIVYPWIQYIR
                     NIPPLAYVPLVVISAGVGLLPQVIVIAIATFLTMTVTIYQGIINIDPNLIKAARILGA
                     SDFEIFKRILVPASIPFIMTAVRLGTSISLTTIIAAESTGATSGMGMRIRSLNNSFDS
                     SGMLLYIILIGVIGLLLNKLVDLIERKLTGWQQK"
     gene            864..>1600
                     /locus_tag="ACHU66_002115"
     CDS             864..>1600
                     /locus_tag="ACHU66_002115"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3390355.1"
                     /translation="MATEVKKIKVKIEEVSKSFQSIHGEVVALNQINLEIFENEIVTV
                     VGPSGCGKSTLLNLVAGLEPVTSGKIYCDDNEIVGVGVERGVVFQQYALFPWMSVQQN
                     VMFGPEMQGKSKLEASEIANKYIKMVGLQEFSNHYPKELSGGMKQRVAIARAYAVNPT
                     ILLMDEPFGALDAQTRIQLQDELIEMLEHERKTCLFITHDIEEAILLGNRVIIMSSRP
                     GEIKEIVNVDLPFPRSQSIKFENDFID"
BASE COUNT          519 a          213 c          317 g          551 t
ORIGIN      
        1 tgtcttagtt aaaacggtaa tttttgtagc tatactaatt aatagtttgc aaaataggat
       61 tattttaatt tattagggct aaacattttt taaagtattt gtaactatgg gaggataaag
      121 atgaagagca acaaagaaca aaaatatttt tggttgacat tctcattgat tgttgcattg
      181 atgatttggt ggtttttatc gaattatgat cgaacctctc gtagttttcc aaatatcatt
      241 ttggtttgga atgctttatt aatcatgata gacaaagggt ttatctttga agatatattt
      301 tcaagtctaa cttcagtttt cttcgggttc acgtcgggct ttctattgtc ggtgcctaat
      361 gcattgttta tgtcctggta ttctccatat cgtaacattg tatatccttg gatacaatat
      421 atcagaaata tcccaccact tgcgtatgtt cctttagtag taatttcggc tggggtcggg
      481 ctgttacctc aagttattgt aatagcgata gctacttttc tgactatgac tgtaacaatt
      541 tatcaaggca ttattaatat tgatccaaat ttaattaaag cagcaagaat tttaggtgca
      601 tcagattttg aaatatttaa aagaattttg gttccagcat ccattccatt tattatgaca
      661 gctgtcagat taggtacatc gatttcttta actacaatca ttgcagctga atctactggt
      721 gctacatcag gtatgggaat gagaatacgg tctttaaata actcttttga ttcctcagga
      781 atgcttttgt atatcatatt gattggagta attggtttac tgttaaataa gttagttgat
      841 ttgatagaaa ggaagttaac tggatggcaa cagaagtaaa aaaaattaag gtcaaaattg
      901 aagaagtttc aaaatcattt cagtcaattc atggtgaggt tgttgcacta aatcaaataa
      961 atttagaaat ctttgagaat gaaattgtta cagttgttgg tccatcaggt tgtggtaaat
     1021 ctactctact aaacttggtt gcaggtttgg aacctgtaac ttccgggaaa atttattgtg
     1081 atgataatga aattgttggc gttggagttg agcgtggtgt tgtttttcaa caatatgcac
     1141 tatttccttg gatgagtgta caacagaatg taatgttcgg acctgaaatg caaggaaaat
     1201 caaagttaga agctagcgaa atagctaaca agtatattaa aatggttgga ttacaagaat
     1261 tttccaatca ttatccgaaa gaattatcgg gaggaatgaa gcaaagagtt gcaatagcaa
     1321 gagcgtatgc tgttaatccg acgattctat taatggatga accatttgga gccttagatg
     1381 cccaaacgag gattcaactg caagatgaat tgattgaaat gctggaacat gaacgaaaaa
     1441 catgtttgtt tatcacacat gatattgagg aagcaatact tctaggaaat cgagtaatta
     1501 ttatgagttc aaggccggga gaaattaaag aaattgtgaa tgttgattta ccgtttccaa
     1561 gaagtcaatc cataaaattt gaaaatgatt ttatagatat
//
LOCUS       DAZQCJ010000179         1531 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000179 DAZQCJ010000000
VERSION     DAZQCJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.181"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..1229)
                     /locus_tag="ACHU66_002116"
     CDS             complement(<1..1229)
                     /locus_tag="ACHU66_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFR3390356.1"
                     /translation="MNRLMHFLEHKLAPFGTKVGNQRHLKAVRDGFMMAMPLVLVGSF
                     FLLLISWPDQASGGFSVVGFLEQIGLLGILNRLNQSTMAIISLIATFGIAYRLSESYE
                     TDGPSAGVIALSSFILLAPRFSTIVFNESGEQINQVFAGAIPFGSLNASALFVAIAIG
                     LVSAEIYRFFIQRGITIKMPKNVPDVVGKSFAALLPALTTLIFWACVFQGLEAAGVEG
                     GLGAVLGLIVGKPLGLIAGSLGGMVLVVLVNSLFWFTGVNGGQVLNAFVDPVWLQFTE
                     ANQVAAAAGEKLPHIITLPFKDLFVFMGGGGATVGLAICLLVFSKSRTFKSLGRISFI
                     PSLFNINTAILFSFPTVLNPIMMIPFILTPTVNAIITYFAMATGLVPMTTGVVLPWTM
                     PPILGGFLATGGSVQGA"
BASE COUNT          561 a          301 c          240 g          429 t
ORIGIN      
        1 attgctcctt gaacactacc gccagtagca aggaacccac caagaattgg aggcattgtc
       61 caaggaagaa caacccctgt tgtcatcgga actaatccag ttgccatagc gaaataggta
      121 attattgcat ttactgttgg cgtgagaata aaaggaatca tcataatagg attcaaaacg
      181 gttggaaaac tgaacagaat agcagtatta atattaaaga gagaaggaat aaaggagatt
      241 cgtcctaaac tcttaaatgt acgactttta ctaaagacaa gtaagcaaat cgctaatcct
      301 actgtagcgc caccaccgcc cataaatacg aacaaatctt tgaatggcaa agtaataata
      361 tgtggtaatt tttctcccgc agctgctgca acttgatttg cttctgtgaa ctgtaaccaa
      421 accggatcaa caaaagcatt taacacttgt ccaccattaa cgccagtaaa ccagaaaagt
      481 gaatttacaa gcacaacaag caccattcca cctagacttc ctgcaataag tcctagtggt
      541 ttacctacaa ttaatcctaa aaccgctcca agtccacctt caacaccggc agcttctaat
      601 ccctggaaga cgcacgccca gaaaatcaat gttgttaatg ctggaagcaa tgcagcaaac
      661 gacttaccca caacgtcagg gacatttttt ggcattttaa ttgtaatacc tctttgaata
      721 aagaagcgat aaatttcagc tgaaactaat ccaatagcaa tagcaacaaa taatgcactt
      781 gcgttcaaac ttccaaatgg tattgctcca gcaaatactt gattaatttg ttcaccagat
      841 tcgttgaaaa ctattgttga gaatcttggt gcaagtaaaa taaaagaaga taaggcaata
      901 acacctgcag aaggtccatc tgtctcatat gattcggata atcgatatgc aataccgaat
      961 gtagctatga gagagatgat cgccattgtt gattgattta accgatttaa aattcccaac
     1021 aaaccaattt gctctaaaaa gcctactact gaaaatcccc cagaagcctg atccggccaa
     1081 ctgattagca acaggaagaa cgagccaact aatacaagag gcatagccat cataaaaccg
     1141 tcacgaactg ctttcaaatg acgttgattt cctaccttag ttccaaaagg agcaagttta
     1201 tgttcaagaa aatgcattaa tctattcatt ttcttatcct ccattaattt tattttgtaa
     1261 aataaagata tcatgtaagc gcttaattgt caagttttta tttatatttt taattaaaaa
     1321 acaaaattcg tttatacatt cagtattttt acttcatttt ttataaaaaa tatgttattc
     1381 ttacattgaa aaatactatt ttccaatgtg aggtactaaa atgggaaaga aagaagaaac
     1441 cattagaaat atttctggaa atatattaaa aactttattt agacatcaaa aaagctctag
     1501 aacaagaata tctaaatctt taaaaattac t
//
LOCUS       DAZQCJ010000180         1393 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000180 DAZQCJ010000000
VERSION     DAZQCJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.182"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..385
                     /locus_tag="ACHU66_002117"
     CDS             <1..385
                     /locus_tag="ACHU66_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HFR3390357.1"
                     /translation="LAQGILIGIWGTTLLFSFIWYILVAISNYILFKKAGYAGWKSLI
                     PIYNLYIQQCIAFGYEKRWFILFLLIPLAGPLYGIYLVYNFGRSFGLSAVQAIFYVLL
                     TPILNLYIAFNDGSRYQGPQEFFID"
     gene            complement(421..1335)
                     /locus_tag="ACHU66_002118"
     CDS             complement(421..1335)
                     /locus_tag="ACHU66_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFR3390358.1"
                     /translation="MNELELLARALGFIFVIGLGYTLKVKGIVRREDARIFSSLVMNI
                     TLPASLLVASATAQVSIDLYVPFILGILCNLFLDMVGYWEAKKSGQQTSVGLMQLSGY
                     NIGTFTLPFVQAFFPLSSLLSVIVFDAGNAIMVLGGNYSIAAGVDEEQERMTVATFIK
                     NLSRSVPLVVYLVSLSLAVCSIKLPRELISALTIASNANPFLAMLMLGILLDLKLNWQ
                     EISRLVYLLSLRLGANILIAGVIYFLLPIDKTMKIMLLVCLVSPISVMSPIYALKLGS
                     RSAEPANVNSLSILVSLVMMVLLIFLFV"
BASE COUNT          460 a          286 c          252 g          395 t
ORIGIN      
        1 attagcacag ggaattctca ttggcatttg gggtacaaca ctactatttt catttatttg
       61 gtatatcctt gttgctattt ccaactacat cctcttcaaa aaagcaggct acgctggttg
      121 gaaatcattg attccaatct acaatctcta tatccaacaa tgcatcgcct ttggctatga
      181 aaaaagatgg ttcattctat tcttactgat tccgctagca ggaccattat atggcatcta
      241 tctcgtttac aattttggtc gtagcttcgg tttatcagct gtacaagcta tcttctatgt
      301 tcttcttaca cctatcttga acctctacat cgcctttaat gatggaagtc gttaccaagg
      361 tccacaagaa ttctttattg attaatatac aatcaagccc ccgcacacgc ggaggctttt
      421 ttaaacgaat agaaaaataa gaagaaccat catgactagg ctaaccaaaa tagataagga
      481 gttaacattg gcaggttcag ctgagcgact ccctaacttt aatgcataaa ttggggacat
      541 gactgaaatc ggagagacta gacagaccag caacatgatt ttcatcgttt tatcaatagg
      601 tagaagaaaa taaatgactc ctgctattag aatattcgca cccagcctca aagagagaag
      661 atagaccaat cgactaattt cttgccaatt caattttaaa tcaagcaaga ttcctaacat
      721 gagcatggct aaaaaaggat tggcgttact ggctatcgtc agcgcagaga taagttcgcg
      781 tggtagtttg atagagcaga ctgctaacga taggctaact agatagacta ctagtggaac
      841 tgagcgtgaa agattcttta taaaagttgc aacggtcatt ctttcctgct cttcatccac
      901 accagctgcg atactgtaat tcccacctag aaccatgatt gcattccccg catcaaatac
      961 gatgacgctt agtagactag atagcggaaa aaaagcttgc acaaatggga gtgtaaaggt
     1021 tccgatatta tagcccgata actgcataag cccgacggaa gtctgttgac cagacttctt
     1081 tgcttcccag tatcccacca tatccaaaaa aagattacaa agaatcccta atatgaaggg
     1141 aacgtataaa tctatagata cttgggctgt cgctgaagct acaaggagag aagctggtag
     1201 cgtaatattc atcactaagc tagaaaagat tcttgcatct tcccttcgaa caatcccttt
     1261 gacttttaat gtataaccta aaccgataac aaagataaaa ccgagagcac gggctaataa
     1321 ttcaagttca ttcatgaaac tagtatagca tatagaaaag aattttgatg atatataaaa
     1381 attttatata atc
//
LOCUS       DAZQCJ010000181         1383 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000181 DAZQCJ010000000
VERSION     DAZQCJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.183"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            123..278
                     /locus_tag="ACHU66_002119"
     CDS             123..278
                     /locus_tag="ACHU66_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390359.1"
                     /translation="MTYTVTVLFDHMLVDETHYFENEADALKCKAGLEARYRGQRLYS
                     VKMEEVK"
     gene            275..583
                     /locus_tag="ACHU66_002120"
     CDS             275..583
                     /locus_tag="ACHU66_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HFR3390360.1"
                     /translation="MKEIDPLVLVTLCIVIALFAAVTEVKVLREQVKRLEEREMVIIH
                     KVDNAGVTMVGKVTRKDIIDGRYYVEIGAYGKFLVTKGQFETINIGDDIPDYLQGRGS
                     "
     gene            585..800
                     /locus_tag="ACHU66_002121"
     CDS             585..800
                     /locus_tag="ACHU66_002121"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390361.1"
                     /translation="MTKTQEPCLAKIGKYWERADFLGVFQHSGTNLIFGHQFAGPVAV
                     VRFRGRLVKVEIENIDFCEVENEQAAT"
     gene            989..1303
                     /locus_tag="ACHU66_002122"
     CDS             989..1303
                     /locus_tag="ACHU66_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390362.1"
                     /translation="MTLFDEVQQLSSESYDKWFERYFKKYDLEKVIKKSAMQGYSGHL
                     ISVLKVKDDYTRRRLDDERTLEKIKELLGDGFKVEYHSTYSKNLFTGEDFITNKQIHI
                     SW"
BASE COUNT          497 a          169 c          324 g          393 t
ORIGIN      
        1 gcaagtgcta taaagcccgg tataatagga ttcttgattt ttataaaaaa agtttttaga
       61 aaagtgatgt aaccttgtaa ccgtgaaatt ttatgcaaaa gaatgaataa aaaggagtaa
      121 aaatgacata tacagtaaca gtattatttg atcacatgtt agttgatgaa acgcattact
      181 ttgaaaatga agctgatgct ttgaaatgta aagcagggct ggaagctagg tatcgaggtc
      241 agcggttgta cagtgtgaag atggaggaag ttaaatgaaa gaaattgatc cattggtttt
      301 ggtgactctg tgtattgtca tcgctttatt tgcagcagtg acagaagtaa aagtattgcg
      361 tgaacaagtg aaaagactgg aagaacgtga aatggttatt atccataaag tcgataatgc
      421 gggcgtgact atggtcggaa aagtcacaag gaaggatatt attgacggca ggtactatgt
      481 agaaatcggt gcctacggca aattcctagt cacaaagggt cagtttgaaa cgattaatat
      541 tggtgatgat attccggatt acctacaagg aaggggtagt tgagatgaca aaaactcaag
      601 agccttgctt ggcaaaaata ggtaaatact gggaaagagc cgattttttg ggagtgtttc
      661 aacattctgg gacaaatctt atctttggtc atcaatttgc tggaccagtt gctgtagtta
      721 gatttagagg tagactggta aaagtagaga ttgagaatat tgatttttgt gaggtggaaa
      781 atgaacaagc ggcaacgtaa aaagaaaata ctgaatggat tgagtacaga agaaagatac
      841 cgtagaacgc attgccctgt ttgtaaagaa aaaatcggag tatttgacaa atattttaat
      901 acatacggtt tttgctctga atattgtggc tatgaatact atggtatttc aagattataa
      961 aactgaataa tgaaaggatg ggattgaaat gacactattt gatgaagtac agcaattaag
     1021 ttcagaaagc tatgacaagt ggtttgaacg ttactttaaa aaatatgatt tggaaaaagt
     1081 cattaagaaa tcagctatgc aagggtatag tggccatctg attagtgtct taaaagttaa
     1141 agatgattat acgagacgaa gattggatga tgaaaggacc cttgagaaaa tcaaggaatt
     1201 gttaggagat ggtttcaaag tagagtatca ttcgacctat agtaaaaatt tgtttactgg
     1261 tgaagatttt atcactaaca agcaaattca tatttcatgg taacaaaaaa gccaaggcac
     1321 tctctgccca ggctgtggtt tcgctatcaa tattatacca caaaggagaa agagagtgaa
     1381 caa
//
LOCUS       DAZQCJ010000182         1375 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000182 DAZQCJ010000000
VERSION     DAZQCJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.184"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..603
                     /locus_tag="ACHU66_002123"
     CDS             <1..603
                     /locus_tag="ACHU66_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR3390363.1"
                     /translation="FLLVTIYNQPKYALNTFFERGVIGTGVQRDFNILFMPVFSMNIL
                     LILFRPMITQLAIYRRAGDYNQFKQYQKRIVKMVVGLAVLVLVGGIVLGIPALNILYG
                     TNLNKYWLSFIITMLGGIASTFATICDNMLTVLRKQKYLVISFAISCLLSILISNPLV
                     EYYGILGAAIAFVSSMWTWFLISFVIYLKLQKHLNWEVQE"
     gene            600..>1375
                     /locus_tag="ACHU66_002124"
     CDS             600..>1375
                     /locus_tag="ACHU66_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HFR3390364.1"
                     /translation="MKIEKLFLRLAIPFLLISIFVMPVTKVPDEATHAFMSWNILFDS
                     PTSRDTEAMNELRAADFAYSEPEIHAVDSTEYSSLFTKIRDFSKDKLSIDFSLKSLMS
                     TPQLIGLVVGKLIYPSYGMMVTIGRLFNTIVYIIGIYYLIKKIKYGKMTLFFVSLLPM
                     MIQQAGSLSYDVVNYLAVVAFFVFYVNLLVDKVLTTRKFINLILLALLLYLTKANNIL
                     LLALLFFVDFEFEGILSKFNLILKWIQKRRLPILILGLGLV"
BASE COUNT          408 a          185 c          255 g          527 t
ORIGIN      
        1 tttttacttg ttactatcta taatcaacct aaatatgctc tcaatacttt ttttgagaga
       61 ggtgttatag gaacaggtgt tcaaagagat tttaatattt tatttatgcc tgttttttca
      121 atgaatattt tgttgatttt gtttagaccc atgataactc agttggctat atatagaaga
      181 gctggggact ataatcaatt taaacaatat cagaagcgta tcgttaaaat ggttgtcgga
      241 ttggctgtgt tagttttggt tgggggaata gtattgggga ttccagcatt aaatattctc
      301 tacggaacga atcttaataa atactggctc agctttatta taacaatgct tgggggaatt
      361 gcgagtacct ttgcaaccat ttgtgataac atgcttactg ttttgaggaa gcaaaagtat
      421 ctcgtaattt catttgcaat ttcttgttta ttatccatat tgatttctaa tccactggta
      481 gaatattatg gaatattggg ggcagctatt gcttttgtta gttcgatgtg gacttggttt
      541 ttaatttctt tcgtaatata tctaaaattg caaaaacatt tgaattggga agtacaagaa
      601 tgaagataga aaaattattt ttaaggttag caatcccctt tttgttgatt tcaatatttg
      661 ttatgcctgt gacaaaggtt cctgatgaag caacacatgc ttttatgtcg tggaatatat
      721 tatttgattc tccgacaagt agagatactg aagcaatgaa tgaattacgg gcagcagatt
      781 ttgcgtattc agaaccggag attcatgcag ttgattctac agaatattca agccttttta
      841 ccaaaattcg tgatttttca aaggacaaat tatctatcga tttttctctg aaatcgttaa
      901 tgagtacacc tcagctaatt ggtctagttg taggtaaatt gatttatccg tcatatggta
      961 tgatggtcac tatagggcgc ctttttaata caatagtcta tattataggt atctactatt
     1021 tgataaaaaa aataaagtat gggaagatga ccctgttttt tgtctcactt ttacctatga
     1081 tgattcagca ggctggctca ctgtcttatg atgtggtgaa ctatctggct gttgttgcat
     1141 tttttgtttt ttatgttaac ttattggtag acaaggtact gaccaccagg aaatttatta
     1201 acttaatatt actagccttg ctactatatt taacaaaagc aaataatatt ttacttttag
     1261 cactattgtt ttttgttgat tttgaatttg aaggcatctt atctaagttt aatttgattt
     1321 taaaatggat tcaaaagaga agattaccaa ttcttatttt gggattagga ctagt
//
LOCUS       DAZQCJ010000183         1370 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000183 DAZQCJ010000000
VERSION     DAZQCJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.185"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(89..490)
                     /locus_tag="ACHU66_002125"
     CDS             complement(89..490)
                     /locus_tag="ACHU66_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390365.1"
                     /translation="MMKNKGGRPTKMTQGTVKKLEEAFLRGLSDEEACLYANISKPTL
                     YDYCKKKPQFTDRKELLKQRVKTRAKLNISKAIEDGNVDLSKWYLERKDAEFKTKTKL
                     EHDGMVSVAPHNPFEDLTVEELRAIIAEDAG"
     gene            complement(540..1082)
                     /locus_tag="ACHU66_002126"
     CDS             complement(540..1082)
                     /locus_tag="ACHU66_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390366.1"
                     /translation="MTLKTFSDTPNTFTFNYTFKDHDTAQVAGHALMGYMTGTFEQPA
                     IEVHYKSDKVGGDYNRLAVEYVADTELTETFQRICDSFQDYYTDPEKKLEDQYRLERT
                     EQLKQSEDFDSLLKKVVAYELELIDYGERLLSDDPIPMDSETGYSTLDLIGAKGVGLF
                     KSLDEDNEYSGLAYYNAEAE"
     gene            complement(1103..1276)
                     /locus_tag="ACHU66_002127"
     CDS             complement(1103..1276)
                     /locus_tag="ACHU66_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390367.1"
                     /translation="MKIKLFKKLPKESLKDFEERVNEFMATVEVVDVKITMAPAGHSD
                     SFGTVTHILVLYK"
BASE COUNT          323 a          311 c          242 g          494 t
ORIGIN      
        1 taatcacgcg cttccatacg ttcgtctatt cgtcgtttta gaaattgccg aagtacttca
       61 cctagataac cctctcaacc aatagtattt atcccgcatc ttcagcaatg attgctcgta
      121 actcttcaac cgtcaaatct tcaaatggat tatgaggcgc aacggatacc ataccatcat
      181 gttcaagttt tgtcttagtc ttaaattcgg catctttccg ttctaggtac cattttgaca
      241 agtccacatt cccgtcttca atcgcttttg atatatttag tttagctcgt gttttaacac
      301 gttgcttaag tagttctttt cggtcagtaa actgtggttt tttcttgcaa taatcataca
      361 gggttggttt tgaaatattc gcatacaaac aagcttcttc atcgcttagc cctcttagaa
      421 atgcttcctc taatttcttt accgttcctt gtgtcatttt tgtaggtcta ccgcctttat
      481 ttttcatcat gttattcctc tctaatttta gcacaaaaag aggcggttgc ctctctctgc
      541 tattctgcct cagcgttgta ataagcaagc ccgctgtatt cgttatcttc gtccagtgac
      601 ttgaatagcc ccacaccctt agcaccaatt aagtctagtg ttgagtagcc tgtctctgaa
      661 tccattggaa tagggtcatc actcagcaaa cgttctccat agtctattaa ctccagttca
      721 taagccacca cctttttcag taggctgtca aagtcctctg actgtttgag ttgttccgtg
      781 cgttccaggc ggtactggtc ttcaagtttt ttctcagggt cggtgtagta atcttggaaa
      841 ctgtcacaaa tccgctggaa tgtttcagta agttcagtat ctgccacata ttccaccgcc
      901 aaacggttgt aatctccgcc cactttgtca ctcttataat gcacttcgat agctggttgt
      961 tcaaatgttc ctgtcatgta gcccataagc gcgtgccctg caacttgtgc ggtgtcgtgg
     1021 tctttgaagg tgtagttaaa tgtaaatgtg tttggtgtgt ctgaaaatgt ttttaatgtc
     1081 atgttatcat tctccgtttc tgttatttgt ataagactag tatatgtgtt actgtgccaa
     1141 agctgtcaga atgccccgcc ggtgccatag tgatttttac gtcaaccact tcaacggttg
     1201 ccatgaactc gtttactcgc tcttcaaaat ctttcaggct ttctttgggc aattttttaa
     1261 atagtttaat tttcatgttt tccctcactt taagataaat tatgtaaact ttttgaatgt
     1321 agtagttctt ttaaaaaaag aataactaca ctgtcaaacc cttgctccgt
//
LOCUS       DAZQCJ010000184         1336 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000184 DAZQCJ010000000
VERSION     DAZQCJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.186"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..507
                     /locus_tag="ACHU66_002128"
     CDS             <1..507
                     /locus_tag="ACHU66_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR3390368.1"
                     /translation="KRWRSKSIGTIIFDYYKEKNGELFANDQDALNGALKEEIKTLSI
                     TYNYFNIFDVYPYRTLEKLSRPSTFISKEEFVKIRKQPRIVHFLGEERPWRIGNKHRF
                     REDYVSALNQTPWRGTQFESGWQFYFFCFNLFNMVMKPFPMLRYKIITVLIPVFMKYR
                     KIRLQKGV"
     gene            510..>1336
                     /locus_tag="ACHU66_002129"
     CDS             510..>1336
                     /locus_tag="ACHU66_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR3390369.1"
                     /translation="MKFSVIIPAYNVADYLEECVYSVLNQTYEEFEILLVDDGSTDGI
                     TSNICDKLAAKDDRVKVFHQTNGGQSIARNTGIKNASGDYILFLDGDDFWTDVHFLDE
                     INEELHSHEEVVDAVIYPFSYWYGNADIRVRDFPQKLPRHEIITDSVLLVETGALIAP
                     VWNKCVRRELFADSLMFPDGLMYEDGIWCADLLKIITCCCVIENSNYMYRQNRDGSFT
                     NKITQKKVYHAFRGIEENLKNFKQLSNEKQEALLIYLSNSYISILPFVFPYLNNSDI"
BASE COUNT          458 a          169 c          254 g          455 t
ORIGIN      
        1 aaacggtggc gttcaaaaag tataggaaca attatttttg attattataa agaaaaaaat
       61 ggagagcttt ttgctaatga tcaagatgca ctgaatggag cattgaaaga agagataaaa
      121 acactctcaa tcacttataa ttattttaat atttttgatg tttatccata tcgtacgttg
      181 gaaaaattaa gtaggccttc aacgtttatt tcaaaagaag aatttgtaaa aattcgtaag
      241 caaccacgta ttgttcattt tttaggtgaa gaacgtccat ggagaatcgg aaataaacat
      301 cgatttagag aggactatgt ttctgcatta aatcagacgc catggagagg tactcaattt
      361 gagagtggtt ggcagtttta ctttttctgt tttaatcttt ttaacatggt tatgaagcct
      421 tttccaatgt tgcgttataa aattattaca gtcttaattc ctgtattcat gaaatatagg
      481 aaaattagat tacaaaaagg agtctaggtg tgaagttttc cgttattatc ccagcgtaca
      541 atgttgctga ttatctggaa gaatgtgtgt atagtgtact gaatcagact tatgaagaat
      601 ttgaaatatt gttggtagat gacggttcta cagatggtat aacctccaat atatgcgata
      661 aattagcagc taaagatgat agggtgaagg tatttcatca aactaatggt gggcaatcaa
      721 ttgcgagaaa tacaggaata aaaaatgcta gtggtgatta tattttattt ctagatggtg
      781 atgatttttg gactgatgtg cattttttag atgaaatcaa tgaagaacta cactctcacg
      841 aggaagtggt agatgcagta atttatccat tttcatactg gtatggtaat gctgatataa
      901 gagttcgtga tttccctcaa aaattaccta gacatgaaat tattactgat tctgttctac
      961 ttgtagaaac aggcgcattg attgctcctg tttggaacaa gtgtgtccgc agagaactat
     1021 ttgcagattc tttgatgttt ccagatgggt taatgtacga agatggtatt tggtgtgcag
     1081 acctattaaa aataataact tgttgttgtg tcattgaaaa ttctaattat atgtatagac
     1141 aaaatagaga tggaagtttt actaacaaaa ttacacaaaa aaaagtctat catgccttta
     1201 gaggaattga agaaaattta aagaatttta aacaactatc gaatgagaaa caagaagcac
     1261 ttttgattta tttatcaaat tcttatattt ccatcttgcc atttgtattt ccatatctga
     1321 ataattctga tataaa
//
LOCUS       DAZQCJ010000185         1312 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000185 DAZQCJ010000000
VERSION     DAZQCJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.187"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..629)
                     /locus_tag="ACHU66_002130"
     CDS             complement(<1..629)
                     /locus_tag="ACHU66_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR3390370.1"
                     /translation="MMKRYSINKRILRTVLEIVIGTVANVIMLLVLSYVLIFTGQLNT
                     YGTDYHISISDSKSANGIKNQMDYSVFDYVLFDSDNGELVSGKYQKQDLQHYKEVFDT
                     GSSVSEGSILFTEYENSDFILVIRRPTVPEFVNQNFRHISYNLVSYLFFIVLELLLIS
                     ISVIRLLREFTKNFRLIEKISLNMGIPGMDIGRKETEILECNNILLTLY"
     gene            complement(607..1305)
                     /locus_tag="ACHU66_002131"
     CDS             complement(607..1305)
                     /locus_tag="ACHU66_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR3390371.1"
                     /translation="MYKILVVDDDFEIVKLMRTILEMKNYNVTTYQEVRLPIKISDFD
                     GFDLILLDVMMPNVDGLQICKQIRKSVSSPIIFVSAKDSEDDIVSGLNLGGDDYITKP
                     FSVNQLVAKVAAHLKREERYRNAESNESVIRELLPLTFYLQEKLVCVNGEAISLTKRE
                     YDILELLSSKTNKVYTREEIYECVYDDEADALFRSISEYIYQIRVKFGSYGINPIKTI
                     RGMGYKWNDEKIFN"
BASE COUNT          460 a          243 c          161 g          448 t
ORIGIN      
        1 tgataaagcg tcaataaaat gttattacat tctaagattt cagtttcttt acgtccaata
       61 tccattcctg gtattcccat attcagagat attttttcaa ttaaacgaaa attttttgta
      121 aattctctta agagcctgat aacggagata gagatgagta gtaattctag cactataaaa
      181 aacaaataag agactaaatt ataggaaata tgtcgaaagt tttgatttac aaattcgggc
      241 actgttggtc ttctaataac caaaataaaa tctgagtttt catattctgt aaacaatata
      301 gaaccctcgc taaccgaact tccagtatca aaaacttctt tataatgttg taaatcctgt
      361 ttttgatatt ttccagagac caattcgcca ttatcgctat caaacaatac ataatcaaag
      421 acagagtaat ccatttgatt cttaataccg ttagcagatt tactatcact aattgaaata
      481 tgatagtctg ttccatatgt attcaactga cctgtaaaaa taagaacgta gctcaataca
      541 agcaacataa taacatttgc aactgtacca atcactattt caagaactgt ccttaaaatc
      601 cgcttattaa ttgaatatct tttcatcatt ccacttatat cccatccccc ttattgtttt
      661 aatgggatta attccatagg aaccaaactt gactctaatt tgataaatgt attccgaaat
      721 tgaacgaaat aatgcatctg cttcatcatc atagacacat tcatagattt cttctcttgt
      781 ataaacctta tttgtcttac tagataatag ctctaaaata tcatattccc ttttagtaag
      841 agaaatagct tcaccattaa cacacacgag tttttcttga aggtaaaatg ttagtggaag
      901 taattctcga ataacagatt cattagattc agcatttcta taacgttctt ctcgttttaa
      961 atgcgctgca accttggcaa ctagttgatt aacactaaat ggctttgtta tataatcgtc
     1021 accaccgaga tttaagccag atacaatatc atcttcagaa tcttttgcac tgacaaaaat
     1081 aatcggagag gatactgatt tacgaatctg tttacagatt tgcagaccat caacatttgg
     1141 catcataaca tcaagcaata ttaaatcaaa tccatcaaaa tcacttattt ttataggaag
     1201 cctaacttct tgataagtcg tcacattata gtttttcatt tctaaaattg ttcgcatcaa
     1261 ttttacaatt tcaaaatcat catcaactac caaaatctta tacatactag gt
//
LOCUS       DAZQCJ010000186         1196 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000186 DAZQCJ010000000
VERSION     DAZQCJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.188"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(115..>1196)
                     /gene="dnaN"
                     /locus_tag="ACHU66_002132"
     CDS             complement(115..>1196)
                     /gene="dnaN"
                     /locus_tag="ACHU66_002132"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR3390372.1"
                     /translation="RAISTKNAIPILSTVKITVTSEGITLTGSNGQISIEHFISIQDE
                     NAGLLISSPGSILLEAGFFINVVSSMPDLVLDFNEIEQKQIVLTSGKSEITLKGKEAE
                     QYPRLQEVPTSKPLVLETKVLKQTINETAFAASTQESRPILTGVHFVLTENKNLKTVA
                     TDSHRMSQRKLVLDTSGDDFNVVIPSRSLREFTAVFTDDIETVEVFFSNNQILFRSEH
                     ISFYTRLLEGTYPDTDRLIPTEFKTTAIFDTANLRHSMERARLLSNATQNGTVKLEIA
                     NNVVSAHVNSPEVGRVNEELDTVEVSGEDLVISFNPTYLIEALKATTSEQVKISFISS
                     VRPFTLIPNNEGEDFIQLVTPVRTN"
BASE COUNT          366 a          213 c          225 g          392 t
ORIGIN      
        1 tcagatttgg agtgtaaaac acgaacaaat ctgccaatca accactgcgc tgagatgttg
       61 acacgaaccc tgagaagcga ggttggtctt tttgcccagc ctccttaata ctatttagtt
      121 ggtacgaact ggtgtaacca attgaataaa atcttcccct tcattatttg gaatcaatgt
      181 aaatggacgg acagaagaga taaagctaat tttcacttgt tcactagttg tggctttcaa
      241 tgcttctatc aagtaagttg ggttaaagct gattactaaa tcttcacctg atacttctac
      301 agtatctaat tcctcattca cacgtccaac ttctggagaa tttacatgag ccgatacaac
      361 attattagca atttctagtt ttactgtacc attttgggtt gcatttgaaa gaagacgagc
      421 acgctccatc gagtgacgaa gatttgcagt atcaaaaatt gcagttgttt taaactcagt
      481 tggaattaag cggtcggtat cagggtaggt accttctaat aagcgtgtat agaagctaat
      541 atgctcgctt ctaaaaagga tttgattatt tgaaaagaag acttctactg tttcaatatc
      601 atctgtaaaa actgcagtaa attcacggag agaacgactt ggaatgacaa cattaaaatc
      661 atcaccagag gtatcaagga ccaatttccg ttggctcata cggtgtgaat ctgttgcaac
      721 agtttttaga tttttatttt ctgttaaaac aaaatgaaca cccgtaagaa taggacgact
      781 ttcttgtgta gaagctgcaa atgctgtttc attaattgtt tgttttaata ctttggtttc
      841 taacaccaat ggttttgaag ttggaacttc ctgtaaacga ggatactgtt ctgcttcttt
      901 tccctttaat gtgatttcag acttaccact tgtcaaaacg atttgctttt gttcaatttc
      961 attgaagtca aggactaaat ccggcatgct ggatacgaca ttaataaaga aaccagcttc
     1021 taagagaatg gagcctggag aactgatcaa aagtcctgca ttttcatctt gaatagaaat
     1081 aaaatgttca atcgagattt gtccatttga accagttaaa gtgattcctt cactagttac
     1141 tgtaattttt actgttgaaa gaattggaat agcatttttg gtactgattg cccgtt
//
LOCUS       DAZQCJ010000187         1194 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000187 DAZQCJ010000000
VERSION     DAZQCJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.189"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..283)
                     /locus_tag="ACHU66_002133"
     CDS             complement(<1..283)
                     /locus_tag="ACHU66_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390373.1"
                     /translation="MEFFWYFCLTSASIDDRMFISNTTKLKTGVKMRSHLGVRLSYEA
                     KYWLESIQAVIQEKLDAKINEQDIENLEHATKSYLKEVDNELGATSVTLI"
     gene            570..>1194
                     /locus_tag="ACHU66_002134"
     CDS             570..>1194
                     /locus_tag="ACHU66_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HFR3390374.1"
                     /translation="MKTLYHYTTVETLLLILNYKTLRFKSLLYVDDPLEPSTSDFGNL
                     GGFKYVSCWTDTPNSIPQWTMYSDNMTGVCIGISFDKETDVFLTEKFSLSESSEPIDM
                     VNALHPLKSGLLVTNNKYVPSLEQIRYTDDVSLITPRVVSSDDKFTTINLASNGIYKT
                     TDWSFQNEQRFSFQIFPLPIDLVLELMNANKGDLTGIINSFISVKPKE"
BASE COUNT          384 a          181 c          192 g          437 t
ORIGIN      
        1 aaatcaaagt tactgaagtt gcccctaatt cattgtcaac ttctttcaaa taagattttg
       61 ttgcatgttc taaattttct atatcttgtt catttatttt agcgtctaac ttttcctgaa
      121 ttactgcttg tattgattct aaccaatact tcgcttcgta agatagacgt actccaaggt
      181 gtgagcgcat ttttactcct gttttcagtt ttgttgtatt cgatataaac attctatcat
      241 ctatactggc agaagtcaag caaaaatacc aaaaaaattc caaaaaatag cttcatatag
      301 gtccattttg ttgtttgaca aaggaatgaa ctctcaaaat gcttatttaa taggcttttg
      361 tggttagaag ctcttcgcta tcaaactcaa aatccagtgt ccgcaaggac gtgccggttc
      421 gaccccggcc gccggtatag tttaaaagac aaggtttcta agccttgtct ttttgttttg
      481 aggttataaa attttcagtt gagttactaa aaattactta atgttttgtt gtataatatg
      541 ttatataagc aagaggagga gccgttaata tgaagacact ttatcattat acaacagttg
      601 aaacattgct tctgatatta aattataaaa cattgagatt caaaagttta ctttatgttg
      661 acgatccatt agaaccttca acttcagact ttggtaatct aggtggattt aagtatgtgt
      721 cttgttggac tgatactcct aatagtattc cacaatggac aatgtattct gataatatga
      781 caggagtgtg tataggaatt agttttgaca aagaaactga tgtcttttta acggaaaaat
      841 tttctttatc tgaatcttct gaaccaattg atatggtaaa tgcattacat cctttaaaat
      901 caggcttatt agttacaaat aataaatatg tgccttcttt ggaacaaata aggtatactg
      961 atgatgtttc attgatcact cctcgagtag tttcttcaga tgataaattc acaactatta
     1021 atttagcttc aaatggtatc tataagacaa ctgattggag ttttcaaaat gagcaaagat
     1081 tttcttttca aatttttcca ttaccaatag atttagtttt ggaattaatg aatgcaaata
     1141 aaggagattt gactggaata attaatagtt ttatttctgt taaacctaaa gaat
//
LOCUS       DAZQCJ010000188         1194 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000188 DAZQCJ010000000
VERSION     DAZQCJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.190"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..565
                     /locus_tag="ACHU66_002135"
     CDS             <1..565
                     /locus_tag="ACHU66_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HFR3390375.1"
                     /translation="PRYARNKPYSKEITTSIIDFWNTLLDDKKLDFSEKLYFLEKHYI
                     FSFKELGEIFDNLSIDKDSIKTLKRRDFYLLTYLSSKNLWNNFSEWEDNVWYVIDEMS
                     DHEFISFWILQGIIFNGMDYYAFDYKPADKKYTIWIKKYSFDRINEIEDYEDTVIKKI
                     QSRLDLLEIKGYRFEKRIDERYKRDYD"
     gene            744..>1194
                     /locus_tag="ACHU66_002136"
     CDS             744..>1194
                     /locus_tag="ACHU66_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HFR3390376.1"
                     /translation="MEKHRVRQHIVPQAYLKKFAQKNVNNSGANISVYQKANNRQFNC
                     AVKNVAFEKNYYDVTNKENTKYWEEHFATHIEPLYGNELTNIISSIMLSNSNENVLNP
                     SQKFSLAMMIVFQIIRVPKFLKGRFQNGEVIFDETIRELIQLMPMEFI"
BASE COUNT          460 a          146 c          210 g          378 t
ORIGIN      
        1 tccaagatat gctcgaaata aaccatatag caaagagatt actaccagta ttattgattt
       61 ttggaatact ttattggatg ataagaaatt agatttttca gaaaaactat attttttgga
      121 aaaacattat attttttcat tcaaggagct aggagaaata tttgataatc tatccattga
      181 taaggactcg atcaaaacac taaaacgtag ggatttctat ttgctaactt acttatccag
      241 caaaaatctc tggaataatt tttccgaatg ggaagacaat gtttggtatg ttattgatga
      301 aatgagtgat cacgagttta tttctttctg gatattacaa gggattatat ttaatggcat
      361 ggattattat gcttttgact ataagccagc tgataagaaa tatactatct ggataaaaaa
      421 atatagtttt gataggataa atgaaattga agattatgaa gacactgtta taaaaaagat
      481 tcaatcacgg cttgatttgc tagaaatcaa ggggtatcgg tttgaaaaga gaatagatga
      541 aagatacaaa cgtgactatg attaatatat gatttgttat gaagacggtc tcacattttt
      601 tctaaaataa taggctgaaa tattcattcg ctgaacagaa tcttttgatt tatccactgg
      661 taaaagacgg aatttagttg gaatgggcta atcaagtaaa aaacagaatt tcattataaa
      721 actttttaag gaagattaag tggatggaaa agcatagagt tagacaacac atagttcctc
      781 aagcatattt aaaaaaattt gcacaaaaga atgtcaacaa tagcggagca aatatatctg
      841 tctatcaaaa agcaaataat agacagttta attgtgcggt caaaaatgtt gctttcgaaa
      901 aaaattacta tgacgtaacc aataaagaaa atacaaaata ttgggaagag cattttgcaa
      961 cacacattga gcccttatac ggcaatgagc taacaaatat aatttctagc attatgcttt
     1021 ctaattcgaa cgagaacgtg ttaaatccaa gtcagaagtt ttctttagca atgatgattg
     1081 tatttcaaat aatcagagta ccaaaatttt tgaaaggaag atttcaaaac ggagaagtga
     1141 tttttgatga aacaattagg gagttgatac aactaatgcc tatggagttt attt
//
LOCUS       DAZQCJ010000189         1116 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000189 DAZQCJ010000000
VERSION     DAZQCJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.191"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            176..931
                     /locus_tag="ACHU66_002137"
     CDS             176..931
                     /locus_tag="ACHU66_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390377.1"
                     /translation="MALGNVEKDTEGWIELINQYLQYCIEIGLSPYTQATYKVALTKV
                     LGVSSTNFIATQPRTRANRMNNRVLHKDYRLSNKNNDYWHKVVTSTGLRKSELIHVTG
                     DALQRGRDGRWYLNLAGHKHHTKGRRDRWSPIMATSQEEEEWLVAIFQRAGEKKVFHV
                     PKDLILDDFDGKKVPTALKSHKYPTEYAERVYRSVAREISKIRNRKEVIHLRKELVGI
                     SLNRKACKIVIKTLGHNRPEEFPRSYAYILLKR"
     gene            1020..>1116
                     /locus_tag="ACHU66_002138"
     CDS             1020..>1116
                     /locus_tag="ACHU66_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR3390378.1"
                     /translation="MLPQNNSPLLLNRQQAAELLGIDPKSFDKYIR"
BASE COUNT          381 a          222 c          212 g          301 t
ORIGIN      
        1 caatcttcgc aagcaggttt tgcgcggttc gcaaaacatc ggttcgttag aaccgactgc
       61 aaggaaaaaa gaaagcattg agttaatcca cactcatcgt acactccata actattcagg
      121 ttcatggcat tgttttgcaa aatgggtttc aattaacatt tcgccagaac agttaatggc
      181 attaggtaat gttgaaaaag atacggaagg ttggatagaa cttatcaatc aatacttaca
      241 atattgtata gagattggtt taagtccata tacacaagct acctacaaag tagcattgac
      301 caaagttctc ggagtttcct caactaattt catcgctaca caaccacgta ctcgtgcgaa
      361 tcgtatgaac aatcgtgtat tacataaaga ctatcgttta tccaacaaaa ataacgacta
      421 ctggcataaa gtagttacct cgacaggctt acgcaaaagt gaattaatcc acgtcactgg
      481 cgatgctctg caacgcggcc gtgatggccg ttggtattta aatttagccg gccataagca
      541 tcacacaaaa ggacgcagag atcgctggtc tccaatcatg gccactagtc aagaggagga
      601 agagtggtta gtcgctattt tccaacgagc aggagaaaag aaagtctttc atgttcccaa
      661 agatttaatc ctcgacgatt ttgatggaaa gaaagttcca acagcattaa agtcacacaa
      721 atatcctaca gagtatgcag agcgtgttta tcgcagtgta gcacgcgaaa tctccaagat
      781 tagaaatcgt aaagaagtta tccatctgcg taaagagtta gttggcatct cactcaacag
      841 aaaagcttgt aaaattgtga taaagactct gggacacaat agaccagagg aatttcctcg
      901 ttcctacgct tatattttat tgaaacgtta ggtaacattg aacaattttc aaaaatctag
      961 cctcaattca ttacgcatgt ggcataatac ttgtatctag aaaatgaaag gaattctata
     1021 tgttacctca aaataattct cctcttctgt taaatagaca acaagcagcg gaactacttg
     1081 gaatagatcc taaatcattt gataagtaca tcagaa
//
LOCUS       DAZQCJ010000190         1103 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000190 DAZQCJ010000000
VERSION     DAZQCJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.192"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            207..1049
                     /locus_tag="ACHU66_002139"
     CDS             207..1049
                     /locus_tag="ACHU66_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFR3390379.1"
                     /translation="MFTISNIRERARQTLNETPGIYQVAVIPVTISVIVQLISFSRNS
                     LAGVSAEELASPSYFISTSLFPVFYGLLLGMLSLSVIWTIFNIIKGGKTTTSFKDSLS
                     IFNHKDFWKIFRTYILKSFLLFLWGLLFYLGIGLLIGAGTMITIIAASSQSLDPDALP
                     MEILAILGLMAMGGILLMVLGTALYIPQIYAYSQVEYILFEQLEREEYTDAFSIIRSS
                     RKLMKGYKFKRFTLDLSFIGWFLLVIITFGLAGLYVWPYHYAAQMHFHEEILDDQAKK
                     MSYV"
BASE COUNT          306 a          223 c          196 g          378 t
ORIGIN      
        1 tttatctttg attgcttcga cgagcgagga aacttcgttt ccttatttcc aacttcaaac
       61 actccactag agtatttgaa ctcgctctgc cgtacttcat aaaagcaaac taaacgacta
      121 taccacagca ccttccagtc tatcaaaaaa agcctagctt gtcatctaga attatgttac
      181 aatgttaaga taaggaggtt atgtagatgt ttacaatttc taatatacgt gagcgggctc
      241 gccaaacgct aaacgagaca cctggtatct atcaagtggc cgtcatcccc gtcactattt
      301 ctgttatcgt tcagttaatt tctttctctc gcaatagcct tgctggagtg tcggctgagg
      361 aacttgctag tccgagctac tttatttcta cctctttgtt tccagtattt tacggattgc
      421 tactaggaat gttgtcatta tcagtcatat ggactatctt caatatcatc aaagggggca
      481 aaactaccac atcattcaaa gattccttga gcattttcaa tcataaagat ttctggaaaa
      541 tctttcgaac ttacatttta aaaagtttct tgctcttcct ttggggacta ctgttttacc
      601 ttggtatcgg tctacttatc ggtgctggaa cgatgattac gattatagct gcatccagtc
      661 aatccttaga ccctgatgca cttcctatgg aaatccttgc catactaggg ctaatggcca
      721 tgggaggtat cctacttatg gttttaggaa ctgctcttta tattccacaa atctatgctt
      781 actcacaggt agaatatatc ttgtttgaac aattggaacg cgaggaatat acagacgcct
      841 tttcaatcat tcggtctagc cgaaagttga tgaaaggtta caaattcaaa cgctttacac
      901 ttgatttatc tttcattggt tggttccttc ttgtcattat tacatttgga ttggcaggtt
      961 tatatgtatg gccgtatcac tatgctgcac aaatgcactt ccatgaagaa attttagatg
     1021 atcaggctaa gaaaatgagt tacgtataaa ataagagcga tatttgctct tatttttata
     1081 tacacttttg tgttgatatt taa
//
LOCUS       DAZQCJ010000191         1029 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000191 DAZQCJ010000000
VERSION     DAZQCJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.193"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            176..646
                     /locus_tag="ACHU66_002140"
     CDS             176..646
                     /locus_tag="ACHU66_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFR3390380.1"
                     /translation="MEFEKVYASVKGIVNKARKEFYIKLWDRDDWEQEGMMTLFELLE
                     AQPWLVDEQVQLYCYFKVKFRNRIKDRIRKQESQKRKFDRMPHEDIHELSHAIQSPGL
                     INDELLMLRGALRDYRKNLSNDQLDKYEKLISGQCFNGRREMIRDLQIHLKDFR"
BASE COUNT          402 a          148 c          198 g          281 t
ORIGIN      
        1 tagaactata ccaaatcact cccttctgaa gcagaatgca atgtttgcca tagagtatca
       61 atagcaaata tacttaaaaa aagaaaaatc aaataaagta tgctagacaa aaatgtcctg
      121 tagaaagaca gcacttttcg ataaacttaa accatcaaca aaaggagatt aagtaatgga
      181 attcgaaaaa gtgtacgcaa gcgtcaaagg tattgtaaat aaggctcgaa aagagtttta
      241 cattaaacta tgggatcgag atgattggga acaagaagga atgatgacct tgtttgaatt
      301 gttggaagct caaccgtggc tagttgatga acaagttcaa ttatattgtt attttaaagt
      361 caagttcaga aatcgaatca aggatcgtat ccgcaaacag gaaagtcaaa aacgcaagtt
      421 tgaccgtatg ccacatgaag atattcacga attatctcac gcaatacaat caccgggatt
      481 aataaacgat gaactattaa tgctaagagg tgccttgaga gattatcgaa aaaatctgag
      541 taatgatcaa cttgataaat acgaaaaatt aattagcgga caatgtttta atggtcgccg
      601 tgaaatgata cgtgatttac aaattcattt gaaagacttt cgctaaagct agtcactaac
      661 atcatgttaa tcgaattaag aaattgggca gaagcaccat aaaaagttct gttcagtttc
      721 tttttttata ggttatagca aagagaagaa ggaatagggg gaaaaaaaca aatttcaagt
      781 taaaattagc catcctatct tatgaagagt aggggtgtga ctaaatgtga tactcagcca
      841 atagtaacct aagtaattga aagactgatt cagcggtatt atttagaatt aaccttatgc
      901 tttagtatct acatagaact ttcagtgtaa aaaaatccca aaaatcggtt gacaatgtat
      961 ataagtcgtg atagaataaa tgagttgtct cttgagggac tggttaacag aggaacgaaa
     1021 aaaagtttc
//
LOCUS       DAZQCJ010000192         1015 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000192 DAZQCJ010000000
VERSION     DAZQCJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.194"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..958
                     /locus_tag="ACHU66_002141"
     CDS             <1..958
                     /locus_tag="ACHU66_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR3390381.1"
                     /translation="GKLLVGNFVSLIQAITRVNTYSQSMIQNTYIIYNTSLFMEQLFK
                     FFDMEVSNISIQNSRISRIEKRIDKIKVNNLSFIYPGSVKKTLEDINVEFNKGELVAI
                     VGKNGSGKSTLVKLLSGLYQPSEGQIYYNNESSDVLDLSYYQNNVSVLFQDFVKFELS
                     VRENIGLSDVNSISDSKIKKHIDNLKLNFLTAENNFNLNQRLGTWFNDSRQISGGQWQ
                     KVALARTFFKNASIYILDEPSSALDPVSEKEIFDEFVTRSKDKIALFVSHNLMAASRA
                     DRIIVMQDGRIIDEGKHDDLISKSKYYRELYYSEKYEEESDG"
BASE COUNT          371 a          131 c          186 g          327 t
ORIGIN      
        1 cggtaaactt ttagtaggta attttgtaag ccttattcaa gcaataactc gtgtaaatac
       61 ctactcacaa tctatgatcc aaaatactta tatcatctat aacacaagtt tattcatgga
      121 acaattattt aaattttttg atatggaagt aagtaatatt tcaattcaga atagtagaat
      181 ctcaagaatt gaaaagcgca ttgacaaaat aaaagtcaat aatctctctt ttatctatcc
      241 aggaagtgtc aaaaaaactt tagaggatat taatgttgag tttaataagg gtgaacttgt
      301 agcaattgtg gggaagaatg gttctggtaa gagtacctta gtaaaattac tatcgggatt
      361 ataccaacct tcagagggtc aaatatatta taataacgaa tctagtgatg tcctagactt
      421 gagttattat caaaataatg tttctgttct ttttcaggac tttgtgaagt ttgaattatc
      481 tgtgagagaa aatattggcc taagtgatgt aaacagtata tctgactcca aaataaagaa
      541 acatattgat aacttaaaat taaatttctt aaccgcagaa aacaatttca accttaatca
      601 acgtttaggg acctggttta acgatagtag acaaatttca ggaggacaat ggcaaaaagt
      661 agcattggct aggactttct tcaagaatgc aagcatatac attttggatg aaccaagttc
      721 agcgcttgat cctgtttcag agaaagaaat atttgatgaa tttgtaactc gttctaaaga
      781 taagattgct ttgtttgtgt cacataatct aatggctgcg agtagagcag atcgaattat
      841 agttatgcaa gatggacgaa ttattgatga aggaaaacat gacgacctta taagtaaatc
      901 taaatattat agggaacttt actattcaga aaagtacgag gaggagtctg atggatgaaa
      961 ataaagttat tattgattta tctgagaaag tatttgctaa atttgatgag cagtt
//
LOCUS       DAZQCJ010000193          997 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000193 DAZQCJ010000000
VERSION     DAZQCJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.195"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..>997
                     /locus_tag="ACHU66_002142"
     CDS             <1..>997
                     /locus_tag="ACHU66_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HFR3390382.1"
                     /translation="TYLYGDKVYTNTNLDFWGNQWDMQKSISTGSKFQHKSSGKATIG
                     VIDSGITYDNPDIYSHIISVQNFTSDLETGIVDNQNVVDKIGHGTSVVGQISSSGEYL
                     GIAPDMKIRMYRVFDEGNAQDQWILKALIQAAKDDVDVINLSLGEYLLRDSTDEDDDT
                     ALINIYQRAINYAHNQGSVVVASVGDEGMDLQNQDELKNYLGSLRGKDYSQVDGTVQD
                     IPAELDNVVTVGSVDDNDLVSSFSNQGNGDIDIYATGGGSQALSKVGYDKWVADKLYE
                     KEWILVPTLEGKYTYAYGTSISTPKVSAALGLIIEKYNLKDQPDEAIRILYDNCFL"
BASE COUNT          344 a          126 c          212 g          315 t
ORIGIN      
        1 acttatttgt atggggacaa ggtttataca aatactaatt tagatttttg gggaaatcag
       61 tgggatatgc aaaagagtat tagtactgga agtaaatttc aacataagtc ttctggtaaa
      121 gcaacaattg gtgtaataga ttctggtatt acatatgata atccagatat ttattcacac
      181 attatttcag tacaaaattt cacatcagat ttggaaacag gaatagttga taaccaaaat
      241 gttgtagata aaattggcca tgggacctct gttgtgggac aaatttcatc aagtggagaa
      301 tatttaggga tagcacctga catgaaaata cgaatgtatc gtgtttttga tgaagggaat
      361 gctcaagacc aatggattct caaagcgctc attcaagcag caaaagatga tgttgatgtt
      421 attaacctta gtttaggaga atatttgtta agagattcca ctgatgaaga tgatgataca
      481 gctttgatca atatctatca acgggcaatt aattatgctc ataatcaagg ttccgttgtc
      541 gttgcttcag taggtgatga agggatggat ttacagaatc aagatgaatt aaaaaactat
      601 cttggtagtt taagaggaaa agattatagt caagttgatg gaactgtaca agatattccg
      661 gctgaattag acaatgttgt tacagttggt tcagtagatg ataatgatct tgtatctagt
      721 ttctcaaatc aagggaatgg cgacattgat atctatgcaa caggaggtgg aagtcaagcg
      781 ctttctaaag taggatatga taaatgggta gctgataaat tatatgaaaa agaatggatt
      841 cttgttccaa cgcttgaagg aaagtataca tatgcatatg ggacttcaat ttcgactcct
      901 aaagtctctg cagctttagg attgattata gaaaagtata atttgaagga ccaaccagat
      961 gaagctattc gaattttata tgataattgt tttctta
//
LOCUS       DAZQCJ010000194          970 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000194 DAZQCJ010000000
VERSION     DAZQCJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.197"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..>970
                     /locus_tag="ACHU66_002143"
     CDS             <1..>970
                     /locus_tag="ACHU66_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR3390383.1"
                     /translation="NDSHDIDSVGKNNMWIKGGKISFKMFQEALLDYTVSVSLFEPNF
                     EQKSYIKGLYIQSRGGDRSFLTGDKLEKDRDFFLTFSPSMNCLIGGRGTGKSTLIDML
                     QFVLSQDCDKQSKLEFLCNHANAFVLYVLEDAEYIIEVSLPDVLQENRDNILQYYGQN
                     RENRYGYPYNYNSDSIKEWTRSQYTKVYKVEGKFFKLVDKTRILEKMFDRRYSVNELV
                     RTADGEKITEFISDLMLKNKNLPRPNYGLRTQTLESFEAKLQELDKYRRVRKDSILKI
                     IDDFNQTQVGKLRICYEQIDRWEVPDFESTLFKSNSTLNFSFENYRI"
BASE COUNT          363 a          123 c          181 g          303 t
ORIGIN      
        1 aatgattcac atgatattga ttcagtcgga aagaataata tgtggataaa aggtggaaag
       61 atttctttta aaatgttcca agaagctttg cttgattaca ctgtatcagt aagtttattt
      121 gaaccaaatt ttgaacaaaa gtcttacata aaaggtttat acattcaaag tcgtggaggc
      181 gatagaagtt ttcttacagg tgataagtta gaaaaagata gagacttctt tttaacattc
      241 tcaccatcta tgaactgttt aattggaggg agaggtacag ggaaaagtac actaatagat
      301 atgttacaat ttgtactttc tcaggattgc gacaagcaga gtaaattaga attcctatgt
      361 aaccatgcaa atgcttttgt actctatgtt ttagaggatg ctgaatatat cattgaggtt
      421 agtttgccag atgtcttgca ggaaaatagg gacaatatct tacaatatta tgggcaaaat
      481 cgtgagaata ggtatggata tccttataac tacaattctg actctatcaa agagtggaca
      541 cgctctcaat atacgaaagt atacaaagtt gagggaaaat ttttcaaatt agttgataaa
      601 actagaatat tagaaaaaat gtttgatagg agatattctg ttaatgagtt ggttagaaca
      661 gcggatggag aaaaaattac agaatttatt tccgacttaa tgctaaaaaa taaaaattta
      721 ccgagaccaa actatggttt aagaactcag actctagaat cattcgaagc taaactacag
      781 gaacttgata aatataggag agttcgtaag gattcaatac tcaaaataat tgatgatttc
      841 aatcaaactc aagttggtaa gttaagaatc tgttatgagc aaattgatag atgggaagta
      901 ccagattttg aaagcactct tttcaaaagt aattcaactc tcaatttctc atttgaaaat
      961 tatagaatta
//
LOCUS       DAZQCJ010000195          933 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000195 DAZQCJ010000000
VERSION     DAZQCJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.198"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            39..>933
                     /locus_tag="ACHU66_002144"
     CDS             39..>933
                     /locus_tag="ACHU66_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR3390384.1"
                     /translation="MRIQQLYYIIKIAETGSMNEAAKQLFITQPSLSNAVRDLEKEMN
                     IKIFLRNSKGITLTKDGMEFLSYARQIVEQTELLEVRYKNPNAKRQLFSVSSQHYAFV
                     VNAFVSLLKETEMEDYELFLRETRTWEIIDDVKNFRSEIGVLFLNHFNRDVLLKLLDD
                     NRLSYTTLFTAKPHVFVSKTNPLAQRKSITLDDLVDFPYLSYEQGIHNSFYFAEEILA
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLEFDDEIELVYI
                     QHEKALLSDMGEKFINYLLEEV"
BASE COUNT          308 a          204 c          154 g          267 t
ORIGIN      
        1 ttttagaata atttgtcaaa tcatgttaca atagaggtat gagaatccaa caattatact
       61 atattatcaa aattgcagaa acaggcagta tgaatgaggc tgccaaacaa ctcttcatta
      121 cccaacccag cctttccaat gcagtacgtg acttggaaaa agaaatgaat attaagattt
      181 ttcttagaaa ctccaaaggt attacactta caaaagatgg aatggaattt ctttcctatg
      241 ctcgtcaaat tgttgaacag acagagcttt tagaagttcg ctataaaaat ccgaatgcaa
      301 aacgccaact cttcagcgta tcttcccaac actatgcctt tgtcgtcaac gcctttgtct
      361 cactcttgaa agagactgaa atggaagatt atgaactctt cctacgtgaa acacggacct
      421 gggaaatcat tgatgacgtt aagaatttcc gctcagaaat cggagtgctt ttcctcaacc
      481 atttcaaccg tgatgttctg ctgaaacttt tggatgacaa tcgactatcc tatacaactc
      541 tcttcactgc caaaccacat gtatttgtca gcaaaaccaa tcctttggca caaagaaagt
      601 caatcactct cgatgatttg gtagatttcc cctatctcag ctacgagcaa ggtattcaca
      661 actccttcta ctttgcagaa gaaattctag cccaggaaca ccataaaaaa tcgattgtcg
      721 tatctgaccg tgccacactc ttcaatctct taatcggtct agatggctat acaatcgcaa
      781 caggtatcct caattcaaac cttaacggag ataatattgt atcaattcct ctggaatttg
      841 atgatgaaat cgaattggtc tatattcaac acgaaaaggc attgctgtcc gacatgggag
      901 aaaaattcat caattaccta cttgaagagg taa
//
LOCUS       DAZQCJ010000196          891 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000196 DAZQCJ010000000
VERSION     DAZQCJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.199"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            379..888
                     /locus_tag="ACHU66_002145"
     CDS             379..888
                     /locus_tag="ACHU66_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390385.1"
                     /translation="MLKYIRALPLVIVAGAVVYFTIFICIDNGDWTYVVTRALGLKEE
                     HANNVSLIVIGTQSLFLTSDWFYKRIRAFLNRRLEKAKGLNRQIYLGYKAGIEFLMAL
                     PKRTILFTFYLLLVVAESVGLIEKAKDYAAVIIFIIAVDRVTKIWPEEKRRMIEFSQK
                     IKKRIKSVG"
BASE COUNT          258 a          142 c          208 g          283 t
ORIGIN      
        1 gattggaagt cttcttcttt ttggggtaag taaaaaatgc taagggtgta cagttgtcgt
       61 acctgaaatt ctagcccctg cttgaccgta cttggtataa catattacaa tatatacaag
      121 aacctgtgtt cacagttcat cgctttcatt ggagtgtttg aaggtggaga taaggaaagg
      181 agattcctga caaattaggt ggtaggcgaa aactgccaca ggagcttttt actcgtcgaa
      241 gcaataaagt gaaactaaat ggtgatagag tcaagtattc cttcaaaaaa ttgccttgat
      301 gagcaaaaac gatttcttgc ataagaacac tttgtttatg aatataggtt ctaagatttg
      361 accaagagag gttattatat gttgaaatat atccgtgccc ttccccttgt tatagtagcg
      421 ggagcggttg tttactttac tatttttatc tgtattgata atggtgattg gacctatgtt
      481 gtgactagag cgctagggct taaggaagaa catgccaata atgtttctct gattgtaatt
      541 ggtacccaat cactttttct aacttcggat tggttttaca agcggattcg tgcctttctc
      601 aatcgtcgtt tggaaaaggc caaagggctt aatcggcaga tttatctggg ctataaggca
      661 gggattgagt ttttgatggc gttacccaaa cgaactattt tattcacatt ctatctactt
      721 ttagtagttg ctgaaagtgt tggtctgatt gaaaaagcca aggactatgc tgcggtaatc
      781 atcttcataa ttgctgttga ccgcgtgacc aaaatctggc cggaggaaaa gcggagaatg
      841 attgagttta gccagaagat taagaaacgg attaagagtg tgggataagt c
//
LOCUS       DAZQCJ010000197          884 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000197 DAZQCJ010000000
VERSION     DAZQCJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.200"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..279
                     /locus_tag="ACHU66_002146"
     CDS             <1..279
                     /locus_tag="ACHU66_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390386.1"
                     /translation="LNTIQDGIDKEQKNIDEIKLLRDKLLAHLDKEMVFSTERLDIGI
                     LYYYFEAIEIKSIYTACIELYNTLYGDNQQQVELPKREIILKRFFLEE"
     gene            complement(575..>884)
                     /locus_tag="ACHU66_002147"
     CDS             complement(575..>884)
                     /locus_tag="ACHU66_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390387.1"
                     /translation="YIDPIILIVLIISYSIYRRCHPNNMDTKQTLKCPKCKSIKLQTY
                     VPTQKRYSRKAGMIGHAINGIEGAMFAMLYEADRTYYRCQHCSYRFSRKENRFISNIE
                     "
BASE COUNT          291 a          120 c          158 g          315 t
ORIGIN      
        1 ttgaatacta ttcaagatgg tattgataaa gaacaaaaaa atattgatga gattaaattg
       61 cttcgcgaca aactcttggc tcacttagat aaagagatgg tcttttcaac agaaagatta
      121 gacattggca ttctatacta ttattttgag gcgatagaaa ttaagtccat atatacagct
      181 tgtatcgagt tatataatac tttatatgga gataatcagc aacaagtcga acttcctaaa
      241 agagaaataa ttttgaaaag attttttctt gaagaataac tcgtattgat tagttttttg
      301 tcttattata ccgataaatt tataaaatac ttgacctatc attttgaatt tgattaataa
      361 attatccatc tataagataa agtaggtcaa attagtttga cctactgatg actagtctat
      421 tttatggtgt taattggtgt cgattttatc caaatcagag gatttcagtc cattttcatg
      481 gaagtttcat tttcgtaaat gttgatataa cgggatttgt aaggggtgag aattcttcgt
      541 tttcaatagc gaaattctct aaaaatcagg gaggttattc gatgttagaa ataaaacgat
      601 tctcctttct agaaaatcgg taactgcaat gttgacaacg ataataagtc cgatcagctt
      661 catagagcat tgcaaacata gctccctcta tgccattgat tgcatgacca atcataccgg
      721 ctttacgaga atatcttttt tgtgttggaa cgtaagtttg cagttttatt gacttacatt
      781 ttggacattt gagcgtttgt ttagtgtcca tattattagg atggcatctg cgatagatgc
      841 tataagagat tattagtact atgaggataa tcggatcgat atat
//
LOCUS       DAZQCJ010000198          879 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000198 DAZQCJ010000000
VERSION     DAZQCJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.201"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(94..>879)
                     /locus_tag="ACHU66_002148"
     CDS             complement(94..>879)
                     /locus_tag="ACHU66_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFR3390388.1"
                     /translation="FYHNYCPDSFKYRRNVGLVKVSDESILVLLLLQAELGMTSQRHF
                     YTICHLFPCGQLLERSRFNRRSKQLIWLVQLIRKAMSEMLPSDKLAIIDSFPVPLCQP
                     VRNHRATIFKGLADIGYNASKHLWFYGFKVHMLVTLSGYILNYVVTPASVHDIKVVYE
                     LLEGCKQSVILGDLGYLSSELKKDLEQEGYHLWAPFRKNMTGSEEHNNWKVMAMRRTI
                     ETCFSELCRLFDIEHTLARSLAGLQSRIEQIILAHNLRYFEMN"
BASE COUNT          273 a          165 c          178 g          263 t
ORIGIN      
        1 attttctgat ttttatgaca atgacttgca aaaaatcaat ttccattaca tttttgaaac
       61 aaaaaactaa aaatatttac tatacccgtg gtgctagttc atttcaaaat agcgtaagtt
      121 atgagctaga ataatttgtt caattcgtga ctgtagtccg gctaggcttc tagctaatgt
      181 atgttcgatg tcaaagagac gacacagttc agaaaaacat gtttcaatag ttcgtctcat
      241 ggccattact ttccaattgt tatgttcttc ggacccagtc atgtttttac gaaacggcgc
      301 ccatagatgg taaccttctt gctctaagtc ctttttgagt tcactgctca ggtagcctaa
      361 atcacctagg ataaccgatt gcttacaccc ttctaatagc tcataaacca ccttgatatc
      421 atggacagag gctggtgtga caacatagtt cagaatataa cctgataagg tcactagcat
      481 atgaaccttg aaaccgtaga accaaagatg tttagaggcg ttgtagccaa tatcggctaa
      541 tcctttaaaa atggtagctc tgtgattgcg aacaggttgg caaagtggca caggaaaact
      601 atctataatg gctagtttat cagatggaag catttcactc atagcttttc ggattaattg
      661 gacgagccaa atcaattgtt ttgaccgacg attgaaacgg cttctttcta gtaactgacc
      721 gcaagggaag aggtggcaga tggtgtagaa atggcgctgt gatgtcatac ctagttcagc
      781 ttgtaacaaa agtaagacaa ggattgattc atctgaaact ttaactaagc caacattacg
      841 ccgatatttg aaggaatccg ggcaatagtt gtggtagaa
//
LOCUS       DAZQCJ010000199          876 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000199 DAZQCJ010000000
VERSION     DAZQCJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.202"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            69..281
                     /locus_tag="ACHU66_002149"
     CDS             69..281
                     /locus_tag="ACHU66_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR3390389.1"
                     /translation="MSEEKFDAKLEQLSGSAKEVIGKLTGDKEIEVEGLVEKGIGKAK
                     ELVEDAKDGLEGAINGIKNAFDKEEK"
     gene            451..>876
                     /locus_tag="ACHU66_002150"
     CDS             451..>876
                     /locus_tag="ACHU66_002150"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFR3390390.1"
                     /translation="MKKIVFVCLGNICRSPMAEFIMKDMTSQFHIESRATSNWEHGNP
                     IYPGTQKIFQKHKIPYDKTKTSQQISQADFEEFDVIIGMDSNNVRDLQKMAPAHAKNK
                     IFQFAEKSVPDPWYTGDFDETYEMVKKGCQDWLDRLQLFD"
BASE COUNT          303 a          160 c          187 g          226 t
ORIGIN      
        1 aagaatagta tgtgattttc tttttagaaa atcatcgccc ccatatgtgt atatcaagga
       61 gggattttat gtcagaagaa aaatttgacg caaaactaga acagctatct ggctctgcaa
      121 aagaggtaat tggtaagctg accggcgaca aggaaatcga agttgaagga cttgttgaga
      181 aaggaattgg caaagcaaaa gagttggtcg aagatgcaaa agatgggctt gaaggtgcca
      241 ttaacggcat caaaaatgct ttcgacaaag aagagaaata agcaaagagt tcaatatggg
      301 taggattgct cgtgcactgt ttgataatga acattacact caatttggtc ttttacctgt
      361 agcccgaaca aaaatatgca agagagggga actcaggcat ttacctgtcg agtctgccct
      421 ctcttttctt atgccacgaa aggagaatct atgaaaaaaa tcgtttttgt ctgccttgga
      481 aatatttgtc gtagccctat ggctgaattt atcatgaaag acatgacaag tcaatttcac
      541 attgaaagcc gtgccacttc aaactgggaa catggtaatc ctatctaccc tggaacacag
      601 aaaattttcc aaaagcacaa gattccctat gacaagacca aaacctctca acaaatctct
      661 caagcagact ttgaggaatt tgatgttatc atcggtatgg acagcaataa tgtccgagat
      721 ttacagaaaa tggcaccagc acatgccaaa aataagattt ttcaattcgc agaaaaatcc
      781 gtaccagacc cttggtatac tggagatttt gacgaaacct acgaaatggt aaagaaaggt
      841 tgccaagact ggttggacag gttacagtta tttgac
//
LOCUS       DAZQCJ010000200          869 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000200 DAZQCJ010000000
VERSION     DAZQCJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.203"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..417
                     /locus_tag="ACHU66_002151"
     CDS             <1..417
                     /locus_tag="ACHU66_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HFR3390391.1"
                     /translation="TVGIFGYFGWFVTQLPLWLIFIDIFIFTLLLFNDGNLKISKTEG
                     LVSLFVLPVQVAIIVAGMYFAWTPTAIGPNAMISQGAQGRYFTPFLVYLFPACLMLKE
                     QVSVSVKQKYLIRLLTGTVVINFIMYLFLIVDYYWL"
     gene            440..>869
                     /locus_tag="ACHU66_002152"
     CDS             440..>869
                     /locus_tag="ACHU66_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR3390392.1"
                     /translation="MNILFTLNDAFVPQVAACMGSIMRTLNEDDTCHFYLFSDGISQQ
                     NKEKLHQFVTDGGNKLTVVELENLESYFDFEVDTNGWASVVLARLLVDKLLPKEVDRI
                     IYLDGDTLVLENIRELWEVDLEGKVLGMCPEPTASSERREG"
BASE COUNT          262 a          122 c          191 g          294 t
ORIGIN      
        1 acagttggta tttttggtta ttttggctgg tttgtcactc aactacctct ctggttaatt
       61 ttcatagata ttttcatttt tacgctatta ttatttaatg atggaaattt aaagataagt
      121 aaaacagaag gtcttgtgtc gttatttgta ctccctgttc aggtagctat cattgtagcg
      181 ggcatgtatt ttgcatggac accgacagct attggaccaa atgcaatgat ttctcaaggg
      241 gcacaaggaa gatactttac tccgttttta gtctatctat ttccggcatg tttaatgtta
      301 aaagagcagg taagtgtatc ggtaaaacag aagtatttga tacggttgct cactggcaca
      361 gtagtcatta actttatcat gtatctattt ttgatagtgg attattattg gttgtaagaa
      421 gatagtggaa gagggaaacg tgaatatatt atttacattg aatgatgctt ttgtcccgca
      481 agttgcagct tgtatgggct caatcatgcg aacattaaac gaagacgata catgccattt
      541 ttatcttttc tcagatggta taagtcaaca gaataaagaa aaacttcatc aatttgttac
      601 cgacggagga aataaattga cagtcgttga attggaaaac ttggaatcat attttgattt
      661 tgaagttgac acaaatggtt gggcttcggt tgtacttgca agattacttg tagataaatt
      721 actacccaaa gaagttgata gaatcattta tttagatgga gatactttag ttttagagaa
      781 tattcgggag ctatgggaag tagatttgga ggggaaagtc cttggaatgt gcccagaacc
      841 gacagcttcg tctgagagaa gagaaggct
//
LOCUS       DAZQCJ010000201          866 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000201 DAZQCJ010000000
VERSION     DAZQCJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.204"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(109..432)
                     /locus_tag="ACHU66_002153"
     CDS             complement(109..432)
                     /locus_tag="ACHU66_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390393.1"
                     /translation="MKTFKKICIDENMKMPNINGIKRVQSFNSDVSVNFLLDDESRDF
                     LKENLPLTGVVIYEPTLKKLAENIIILNRQKHRMSDESRISLMNKEIYQGYRETSFYT
                     SIIEA"
     gene            complement(609..>866)
                     /locus_tag="ACHU66_002154"
     CDS             complement(609..>866)
                     /locus_tag="ACHU66_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390394.1"
                     /translation="AFEKTKSLSLKDWHKLDNEMKHSISSIPKDKDVGTLSVRFFLEN
                     SIITSLESYQKEFMTSEMVRQVRLSYVLKLVIFAYYKKIMQ"
BASE COUNT          280 a          146 c          122 g          318 t
ORIGIN      
        1 gaattagagc aggataagtg atgttgaaca taaacttata aattttcata ataataattt
       61 cctttcataa aaccaacggg ggaggagtta gctcccctta tatcatcact aagcttcaat
      121 tattgaggtg taaaaagaag tttccctgta gccttggtaa atctctttgt tcatcaagct
      181 aatcctagat tcatcagaca tacggtgttt ttgacgatta agaataatga tgttttctgc
      241 taacttcttc aaggttggct catagattac tactccagta agtggcaaat tttcctttaa
      301 aaaatctctg gattcatcat ctaagagaaa gtttaccgat acatctgaat taaagctttg
      361 tacccgctta atcccattga tattcggcat tttcatattt tcatcgatac aaattttttt
      421 aaatgttttc atgtaattgc tcctttttta gaatacgttg ttacagtcaa tattcttaaa
      481 taaattggtg aagctctagc cttcattagc attctccttt ttgtgtttgt gtgtaaatgt
      541 agtattggtt ataaacaaag gtgattaagc tacagttcct caatgttacc atgagaaact
      601 gtaatcctct actgcattat ttttttatag taagcaaaaa taacaagttt tagaacatat
      661 gacaatctaa cttgcctaac catttcagaa gtcataaatt ctttttgata actttccaat
      721 gatgtaataa tagaattttc caaaaaaaat cttacagaca aggttccaac atccttatcc
      781 tttggaatag acgaaatcga atgtttcatt tcattatcta atttatgcca atctttcaaa
      841 cttaaagatt tcgttttttc aaaagc
//
LOCUS       DAZQCJ010000202          865 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000202 DAZQCJ010000000
VERSION     DAZQCJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.205"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            23..>865
                     /locus_tag="ACHU66_002155"
     CDS             23..>865
                     /locus_tag="ACHU66_002155"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR3390395.1"
                     /translation="MAYMPNFASLHDIITGINSGIFKEEGLEVELIEFADGPTIIASL
                     ESGSIDIGNIGPGAHKLAIEGRAEVVAFSQLGNADELIARSDRGIDSIKDLRGKKIGT
                     ASGTSAETILKLALLDANLSEKDVEIIDMDASAIVTAMLSGSIDVAATWSPNTTLIKR
                     ELKEKVVVLADNSRYRDQSPAIASYVTFPGYSEKNNEKISKFLKGLYRAMDYRAEHMK
                     EVSKWVATQIGSDITAVQDQLQDGEWLTSRQMIQLINTGILQQYYEKQQKNLIVSGIV
                     DISRN"
BASE COUNT          301 a          121 c          187 g          256 t
ORIGIN      
        1 agccaatata gataaaataa gaattgcata tatgccaaat tttgcaagtc ttcatgacat
       61 tattactgga ataaattcag gtatctttaa agaggagggg cttgaagtcg aattgattga
      121 atttgctgat ggtccaacta tcattgcatc tttagagtca ggttcaattg atattggtaa
      181 tattggtccg ggtgcccata aattggcgat tgaaggtaga gcagaagttg ttgcatttag
      241 tcaacttggg aatgctgacg aattgattgc tcggtcggat agaggaattg attcaataaa
      301 ggatttgagg ggaaagaaaa ttggaacagc atctgggaca tcagcagaaa caattctaaa
      361 gcttgctttg ttagatgcaa atttgtctga aaaagatgta gaaattattg atatggatgc
      421 ctccgctatt gttacagcga tgttgtcggg atcaatagat gtagctgcaa cttggtcacc
      481 taatacaact ttaataaagc gagaattaaa ggaaaaagtt gttgtattag ctgataattc
      541 acgttataga gaccaatctc cagctattgc ttcatatgta acttttccgg ggtattctga
      601 aaaaaacaat gaaaaaattt ccaagttttt aaaagggtta tatcgtgcaa tggattatcg
      661 agctgaacat atgaaggaag tttcaaaatg ggttgctacc caaatcggtt cagatatcac
      721 agctgttcaa gatcaactac aagatggaga gtggttaaca agtagacaaa tgattcaatt
      781 gattaacact gggatacttc aacagtatta cgagaaacaa caaaaaaatt taatagtatc
      841 tggaatagta gatatctcta ggaac
//
LOCUS       DAZQCJ010000203          818 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000203 DAZQCJ010000000
VERSION     DAZQCJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.206"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            70..738
                     /locus_tag="ACHU66_002156"
     CDS             70..738
                     /locus_tag="ACHU66_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFR3390396.1"
                     /translation="MTYSQDYLDDLLVRMAYHSSGIEGNTISLPETVSIILESTLPGK
                     HKSIREFYEIENHKQAFQALLIALDNGEELSLGLVKDIHALLMDRLQHDRGQFKSVQN
                     AIIGATFKTASPEETPFLMQQWVDNANYRLGLPLEIAELLEVLADVHIQFERIHPFSD
                     GNGRTGRLILMFQSMLKLGAPVLISKEDRALYMELLAEQDVTGLSQLLKRSLEFEQER
                     KKQF"
BASE COUNT          235 a          152 c          200 g          231 t
ORIGIN      
        1 cagttggatc atctgaaact gttgaaaaaa gcattgttgc agcagatgtt tatatagaaa
       61 gaggtcagta tgacatattc gcaggattat ttagatgacc ttctggtacg aatggcctat
      121 cattccagcg ggattgaggg gaataccatt tctctgcctg agacggtttc gattattttg
      181 gaaagtacgc tacctggcaa gcacaagagt atccgtgaat tttacgagat tgaaaatcac
      241 aaacaagcct tccaagctct gctaattgcc ttagataatg gggaagagct gagtttggga
      301 ctggttaagg atattcatgc cttgttgatg gaccgcctcc agcatgatcg ggggcagttt
      361 aagtcggtac aaaacgctat tattggtgcg accttcaaga ctgcatcgcc agaggaaacg
      421 ccttttctca tgcaacagtg ggtggataat gccaactatc gcttgggttt gcctttggaa
      481 atagctgagc tgttagaggt gctggcagat gtgcatattc agttcgagcg tattcaccca
      541 tttagtgatg ggaacggccg gacaggacgg ctgattctga tgtttcaaag tatgttaaag
      601 ctgggagcgc ccgtactgat tagtaaagaa gatcgagctc tttatatgga gttgttagcg
      661 gaacaagatg tgactggatt atcccaatta ctcaaacgct ccttggagtt tgaacaagaa
      721 cgaaaaaaac agttttaagt cgagttttca gaaagcgact ttcaatcgga actattattt
      781 ttgcctgaaa atacaatgaa aaccttactt tcacaact
//
LOCUS       DAZQCJ010000204          800 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000204 DAZQCJ010000000
VERSION     DAZQCJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.207"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..368
                     /locus_tag="ACHU66_002157"
     CDS             <1..368
                     /locus_tag="ACHU66_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PlySs2 family phage lysin"
                     /protein_id="HFR3390397.1"
                     /translation="RYPVRNYYSATSYRQQVVHHITPPGTITQSAPNLAGSRSYRETG
                     TMTVTVDALNVRRAPNTSGEIVAVYKRGESFDYDTVIIDVNGYVWVSYIGGSGKRNYV
                     ATGATKDGKRFGNAWGTFK"
     gene            433..>800
                     /locus_tag="ACHU66_002158"
     CDS             433..>800
                     /locus_tag="ACHU66_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR2 nuclease fold domain-containing protein"
                     /protein_id="HFR3390398.1"
                     /translation="MKYNVKISQKIKEIVGLETSATDILIHEENLEKHMLKSRHRKMI
                     KYIPDVATILDSPDFVGQNKNVKTESFEVIKVLADNVLVAVKLDKKNDYFFVASVYDI
                     TDSKLNHMKRNGRIKAFDKI"
BASE COUNT          260 a          158 c          174 g          208 t
ORIGIN      
        1 aacgttatcc cgtccgtaat tattacagcg ctacaagcta tcgtcaacaa gtcgtgcacc
       61 acatcacacc gcctggaacg attacacagt cagcacccaa ccttgcaggc tctcgttcct
      121 atcgcgagac gggcactatg actgtcacgg tcgatgctct caatgttcgc agggcgccaa
      181 atacttcagg cgagattgta gcagtataca agcgtggtga atcatttgac tatgatactg
      241 tcatcatcga tgtcaatggc tatgtctggg tgtcttacat aggcggcagc ggcaaacgta
      301 actacgttgc gacgggcgct accaaagacg gtaagcgttt cggcaatgct tggggtacat
      361 ttaaataaag tttcagccca gcatttgctg gggattatga tttgtgaaaa ggaatgtggt
      421 ataataaaaa tcatgaaata caatgtaaaa atcagtcaaa aaatcaagga aatagtaggg
      481 ttagaaactt ctgcaaccga tatcctgatc cacgaagaaa atcttgaaaa acatatgtta
      541 aaaagcagac atcgaaagat gatcaaatat atcccagatg ttgctactat tttggattcg
      601 ccggattttg ttggtcaaaa taagaatgtg aagacagaga gttttgaagt tatcaaagtc
      661 cttgcagata atgttctggt cgctgtcaaa ctagataaga aaaacgacta tttctttgtg
      721 gcttcggttt atgacatcac agattccaaa ttaaatcaca tgaagagaaa cggtcgcatc
      781 aaagcatttg acaaaataga
//
LOCUS       DAZQCJ010000205          791 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000205 DAZQCJ010000000
VERSION     DAZQCJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.209"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            5..>791
                     /locus_tag="ACHU66_002159"
     CDS             5..>791
                     /locus_tag="ACHU66_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFR3390399.1"
                     /translation="MMMKKYIMTGLVLLATIGLSACSGNATQEDTSLKDVQEKGKLIV
                     ALNPEFPPFEFRTLVDGKDTIVGADIELAKAIGQELGVEVEFSAMSFDNVLNNVQSGQ
                     SDIAISGISATEERAKVYDFSIPYYTSTNKVIINKEDLAQYTSLDSLAEANIGAQKGS
                     IQEQIIKEQLPSSTVIALASNGELINQLKSQKVEAVIFEEPIAKAYVAKNDDLVILDT
                     EIKSSYSDAYAIALPKGSTALKEKVDSVITKLVESGQMDQFVQE"
BASE COUNT          273 a          125 c          165 g          228 t
ORIGIN      
        1 gaatatgatg atgaaaaaat atattatgac aggcttggtt ttactggcaa caataggtct
       61 aagtgcttgt agtggaaatg ctactcaaga agatacttcc ttgaaggatg ttcaggaaaa
      121 gggaaaacta attgttgcat taaatccaga atttccacca tttgagttta gaacactggt
      181 tgatggaaaa gatacaatcg ttggagctga cattgagctt gcaaaagcca ttggtcaaga
      241 attgggtgta gaagtcgaat tttcagcgat gagttttgat aatgttttga acaatgtcca
      301 gtcagggcaa tcggatattg ccatttcagg aatttcagcc actgaagagc gtgcaaaagt
      361 gtatgatttt tccattcctt actacacctc aaccaataaa gttattataa ataaagagga
      421 tcttgcacaa tatacatcgc ttgattctct agctgaggca aatataggag cacaaaaagg
      481 ttctattcaa gaacaaatta ttaaagaaca gcttccatcc tccacggtta ttgctctagc
      541 ttcgaacgga gagctgatta atcaattaaa atcacagaag gtagaagcag ttatttttga
      601 agagccgatt gcgaaagcct atgttgccaa aaatgatgat ttagttattt tagatactga
      661 gataaaaagt agctattcag acgcctatgc tattgccttg ccaaaaggaa gtacagcttt
      721 aaaggaaaaa gttgactctg tcattacaaa actagtggaa tctggacaga tggatcaatt
      781 tgttcaagaa g
//
LOCUS       DAZQCJ010000206          747 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000206 DAZQCJ010000000
VERSION     DAZQCJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.210"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..671
                     /locus_tag="ACHU66_002160"
     CDS             <1..671
                     /locus_tag="ACHU66_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390400.1"
                     /translation="GFTYVEKDIMIPPLFRKIPFQPILAYPNGHLSTTMITLSVEKNT
                     GLNDSVIQNATLIPLEEIIAKLESSTNEDTQFIINSNVGSKLLFEAFSSSLDSEQLFD
                     ILQNEDAPNTLLEKIYSKIEKSKQRTIFINKRAKQKTVKLTPKTVVKNHPQKSSVEKR
                     RQRYLDKVFSKNPNIQVLEYIDSKTPVHYKCCQCQFEWKARSDKIFIRPYCKNCKSIS
                     IDKN"
BASE COUNT          287 a          113 c          104 g          243 t
ORIGIN      
        1 caggttttac ctatgttgaa aaagatatta tgatacctcc attgtttaga aagattcctt
       61 ttcaacctat tttagcttat cctaatgggc acctctcaac aactatgatt actctttctg
      121 ttgaaaaaaa tacaggatta aatgatagtg tcattcaaaa tgcaactttg atcccattag
      181 aggaaattat tgctaagctt gaaagttcta ctaatgagga tactcaattt attataaatt
      241 caaatgttgg cagtaaactt ctatttgaag cattttcttc ttcacttgac tcagagcaat
      301 tgtttgatat tctccagaat gaagatgctc caaatactct tttagaaaaa atatattcta
      361 agatagaaaa atcaaagcaa cgaactattt ttattaacaa aagagctaaa caaaagacag
      421 taaaattaac gccgaaaaca gtagtaaaaa atcatcccca aaaatcttcg gtagaaaaaa
      481 ggaggcagag atatttggat aaagttttct caaaaaatcc aaatattcag gttttagaat
      541 atattgattc aaaaactcct gtccattata aatgttgtca atgccaattt gaatggaagg
      601 caagaagtga taaaatattc attcgtccct attgtaagaa ttgcaaatca atctccattg
      661 ataaaaatta gattttctag caattcaatt attttaattg tttagataat tttatgatga
      721 aaaaataaaa tcagtcccaa ggctgat
//
LOCUS       DAZQCJ010000207          742 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000207 DAZQCJ010000000
VERSION     DAZQCJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.211"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(1..724)
                     /locus_tag="ACHU66_002161"
                     /pseudo
     CDS             complement(1..724)
                     /locus_tag="ACHU66_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
BASE COUNT          222 a          141 c          162 g          217 t
ORIGIN      
        1 aatagttcgt ctcatggtca ttactttcca attgttatgt tcttcagcct ctgccatgtt
       61 ttgtcgaaat ggcgtccata gatggtaacc ttgttgttct aagtccttct tgagttcact
      121 gctcaggtag cctaaatcac ctaggataac cgattgctta cacccttcta atagttcata
      181 aaccaccttg atatcgtgaa cagaggcagg tgtgacaaca tagttcagaa taaaaccaga
      241 taaggtcacc agcatatgaa ccttgaagcc gtagaaccaa agatgtttag aggcgttgta
      301 gccaatatcg gctaatcctt taaaaatggt agctctgtga ttgcgaacag gttggcaaag
      361 gaaaactatc tataatggct agtttatcag atggaagcat ttcactcata gcttttcgga
      421 ttaattggac gagccaaatc aattgttttg accgacgatt gaaacggctt ctttctagta
      481 actgaccaca aggaaagagg tggcagatgc tatagaaatg acgctgtgac gttatgccta
      541 gttcggcctg taacaagagc aagacaagga gtgagtcatc tgaaactttg gctaagccaa
      601 cattacgccg atgtttaaag gaatctggac aatagttttg gtagaagtga tgacaaattt
      661 gtgataattg gcatacattc cattgcaaat gatgagattt agcggtatac tgtaagtggc
      721 tcatttggac taacctcctg ta
//
LOCUS       DAZQCJ010000208          740 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000208 DAZQCJ010000000
VERSION     DAZQCJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.212"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(43..585)
                     /gene="raiA"
                     /locus_tag="ACHU66_002162"
     CDS             complement(43..585)
                     /gene="raiA"
                     /locus_tag="ACHU66_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR3390401.1"
                     /translation="MIKFSIRGENLEVTEALRTYVEEKVAKIEKYFNEEQELNAKVNL
                     KVYRDKRAKVEVTIPVGAVTLRAEDISQEMYGSIDLVVDKIERQIRRNKTKIERKHRQ
                     KVATGQVFTDELVEQTGEEVKVVRTKQVDLKPMDMEEAVLQLELLGHDFFIYTDANDG
                     TTNVLYRREDGDLGLLEVRQ"
BASE COUNT          191 a          159 c          112 g          278 t
ORIGIN      
        1 aataataaaa cagctgcaaa aactgcaact gttttattat ctttattgac gtacttctag
       61 aagacccaaa tctccatctt cgcgtctata caatacattt gttgtaccgt cattagcatc
      121 tgtatagata aagaaatcgt gtccgagcaa ctccaattgg aggactgctt cttccatatc
      181 cattggtttc aagtctactt gcttagtacg aaccactttt acttcctcac ctgtttgctc
      241 aacaagttca tctgtaaaga cttgaccagt tgccactttt tgacgatgtt ttcgttcaat
      301 cttggttttg ttacgacgaa tttggcgttc aatcttatct actacaagat cgatagagcc
      361 atacatttct tgtgaaatat cttctgcgcg aagtgtaact gctccaactg gaattgtcac
      421 ttctactttt gcgcgcttgt cacggtaaac ttttagattt actttagcat tcaactcttg
      481 ttcttcattg aaatatttct caatcttagc cactttctct tctacatagg tgcgaagtgc
      541 ttctgtaact tcaaggtttt ctccacgaat actgaattta atcatattag tacctctttc
      601 ttggctttcg cctttcttca ctattattat accacattat aggaatattg caaccgtttt
      661 cttatcgtgt tagtgaaaat gttttgattt ctttcctacc aattttcatg aaaagttcta
      721 cagcaagttg gattgtggct
//
LOCUS       DAZQCJ010000209          734 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000209 DAZQCJ010000000
VERSION     DAZQCJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.213"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            113..619
                     /locus_tag="ACHU66_002163"
     CDS             113..619
                     /locus_tag="ACHU66_002163"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFR3390402.1"
                     /translation="MTLYILANPNAGSHTAEHIIFQIKESYPQLAVNIFMTVGPEDEK
                     SQIEAILKEFVSSEDQLMILGGDGTLSKALRFWPASLPFAYYPTGSGNDFAKAMNITS
                     LYRSVDAILEGKTSRIYVLNSSYGTVVNSMDFGFAAQVINGSTNSILKKNSEQGKTWE
                     VNLSILWY"
BASE COUNT          238 a          107 c          139 g          250 t
ORIGIN      
        1 gggcgggaag acgaactctt tttttgacca gtcgagttct ttcccgctcc ctttatttta
       61 tactaaaaaa tagcacctag cttattttta tatatttagt gatggggaat aaatgacgtt
      121 atatatatta gctaatccta atgctggtag ccatactgct gaacatatca tattccaaat
      181 aaaagaaagt tatccacagc ttgcagttaa catttttatg acagttggtc ctgaggatga
      241 aaaaagtcaa atagaggcta ttttaaagga gtttgtcagt agtgaggacc aattaatgat
      301 tttaggtgga gacggcacac tgtctaaagc tttgcgtttt tggccagcta gtctaccgtt
      361 tgcttattat ccaacaggat ctggaaatga ttttgctaag gcaatgaata taacatcgct
      421 ctatagaagt gtagatgcca ttttagaggg aaaaacaagt cggatatatg ttttaaatag
      481 ttcatacgga acggttgtaa acagtatgga ttttggcttt gcagctcaag ttatcaatgg
      541 ttcaacgaat tcaattttga aaaaaaattc tgaacaaggt aaaacttggg aagttaactt
      601 atctattctt tggtattaaa acattatttt caaaacaagc tataaactta gaattaactc
      661 ttgatgaaaa atcttatcag ttagataatc tcttttttat ttctgtagca aatagtcttt
      721 attttggtgg agga
//
LOCUS       DAZQCJ010000210          733 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000210 DAZQCJ010000000
VERSION     DAZQCJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.214"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(105..178)
                     /locus_tag="ACHU66_002164"
     tRNA            complement(105..178)
                     /locus_tag="ACHU66_002164"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(142..144),aa:Arg,seq:acg)
     gene            complement(197..280)
                     /locus_tag="ACHU66_002165"
     tRNA            complement(197..280)
                     /locus_tag="ACHU66_002165"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(244..246),aa:Leu,seq:taa)
     gene            complement(288..359)
                     /locus_tag="ACHU66_002166"
     tRNA            complement(288..359)
                     /locus_tag="ACHU66_002166"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Gly,seq:gcc)
     gene            complement(372..444)
                     /locus_tag="ACHU66_002167"
     tRNA            complement(372..444)
                     /locus_tag="ACHU66_002167"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(409..411),aa:Thr,seq:tgt)
     gene            complement(459..540)
                     /locus_tag="ACHU66_002168"
     tRNA            complement(459..540)
                     /locus_tag="ACHU66_002168"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(504..506),aa:Leu,seq:tag)
     gene            complement(544..616)
                     /locus_tag="ACHU66_002169"
     tRNA            complement(544..616)
                     /locus_tag="ACHU66_002169"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(581..583),aa:Lys,seq:ttt)
     gene            complement(662..733)
                     /locus_tag="ACHU66_002170"
     tRNA            complement(662..733)
                     /locus_tag="ACHU66_002170"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(699..701),aa:Asp,seq:gtc)
BASE COUNT          189 a          216 c          154 g          174 t
ORIGIN      
        1 gaacctgcga caccttggtc ccaaaccaag tactctacca agctgagcta cttcccgata
       61 ctattaaaaa taaagctccc ctaacgtctc cattaagcga gtgatgcacc ctagaggatt
      121 cgaacctcta accgcctgat tcgtagtcag gtactctatc cagttgagct aagggtgcta
      181 actattataa tcttcatgcc gaggaccgga atcgaaccgg tacgatgttg ccatcgcagg
      241 attttaagtc ctgtgcgtct gccagttccg ccaccccggc ctctcaaagc gaacgacggg
      301 attcgaaccc gcgaccccca ccttggcaag gtggtgttct accactgaac tacgttcgca
      361 atctttccta atgccggcta catgacttga acacgcgacc ctctgattac aaatcagatg
      421 ctctaccaac tgagctaagc cggcctattt ctattctatg cgggttaagg gacttgaacc
      481 cccacgcccg aaagcgccag atcctaaatc tggtgcgtct gccaattccg ccaaacccgc
      541 atatgactcg tgctgggctc gaaccagcga cccattgatt aaaagtcaat tgctctacca
      601 actgagctaa cgagtcttta cttaattata ttacttgaaa catatgattg attcgtttca
      661 acggtcccga cgggaatcga acccgcgatc ttcgccgtga cagggcgacg tgataaccgc
      721 tacactacgg gac
//
LOCUS       DAZQCJ010000211          723 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000211 DAZQCJ010000000
VERSION     DAZQCJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.215"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..>723
                     /locus_tag="ACHU66_002171"
     CDS             <1..>723
                     /locus_tag="ACHU66_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR3390403.1"
                     /translation="DNKYLTKIIAEKNPDLIIPLDADEFLTADSNPRKLLEQLDLEKI
                     HYVNWQWFVMTKKDDINESFIPRRMQYCFEKPVWHHSDGKPVTKCIISAKYYKKMNLK
                     LSMGHHTVFGNPNVRIEHHNDLKFAHYRAISQEQLIYKTICYTIRDIATMENNIETAQ
                     RTNQMALIESGVDMWETAREASYSGYDCNVIHAPIDLSFCKENIVIKYNELSRETVAE
                     RVMKTGREMAVRAYNVERKQKEK"
BASE COUNT          278 a          100 c          138 g          207 t
ORIGIN      
        1 gataataaat atctaacgaa aataattgct gaaaaaaatc cagatttgat aatacctttg
       61 gatgcggatg aatttttaac agccgattca aatccacgga aacttttgga acaactggac
      121 ttagaaaaga tacattatgt gaattggcaa tggtttgtta tgactaaaaa agatgatatt
      181 aatgaatcgt ttataccacg tagaatgcaa tattgttttg aaaaacctgt ttggcatcat
      241 tctgatggta aaccagttac taaatgtata atttccgcta agtattacaa aaaaatgaat
      301 ttaaagctat cgatgggaca tcacactgtt tttggtaacc caaatgtaag gatagaacat
      361 cataatgatt tgaaatttgc acattatcga gctattagcc aagagcaatt aatttataaa
      421 acaatttgtt acactattcg cgatattgct actatggaga acaatatcga aacagctcaa
      481 agaacaaatc agatggcgct cattgaatct ggcgtggata tgtgggaaac ggcgagagaa
      541 gcctcttatt caggttatga ttgtaatgtt atacatgcac caattgattt aagtttttgt
      601 aaagaaaata ttgtaataaa atataacgaa ctatccagag aaacagtagc agaacgcgtg
      661 atgaaaacgg gaagagaaat ggctgttcgt gcatataatg tggagcgaaa acaaaaagaa
      721 aag
//
LOCUS       DAZQCJ010000212          708 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000212 DAZQCJ010000000
VERSION     DAZQCJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.216"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..534)
                     /locus_tag="ACHU66_002172"
     CDS             complement(<1..534)
                     /locus_tag="ACHU66_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR3390404.1"
                     /translation="MEKVSIIVPIFNTEKYLRECLDSIISQSYTNLEILLIDDGSSDS
                     STDICLEYAEQDGRIKLFRLPNGGVSNARNYGIKNSTANYIMFVDSDDIVDGNIVESL
                     YTCLKENDSDLSGGLLATFDGNYQESELQKCQIDLEEIKEVRDLGNENFPNHYMSGIF
                     NSPCCKLYKNIYINKGFD"
BASE COUNT          226 a          147 c           85 g          250 t
ORIGIN      
        1 gtcaaaacct ttgtttatat atatattctt ataaagtttg caacaagggc tattaaagat
       61 accgctcata taatgatttg gaaaattttc atttcctaag tctcgcacct cttttatctc
      121 ttccaaatca atttgacact tttgcagctc agattcttga taatttccat caaaagtagc
      181 aagtaaccct cccgacaaat cactatcatt ctcttttaaa caggtgtata aggactcaac
      241 aatgttgccg tcaacaatat catcagaatc tacaaacata atataatttg ctgtgctatt
      301 tttgataccg taattccttg cgtttgaaac accaccattt ggtaaccgga aaagttttat
      361 tctaccatct tgctctgcgt attccaaaca tatatccgtt gatgaatctg aagaaccgtc
      421 atctatcaaa agaatctcta gattagtata cgattgggaa ataatgctat ctaaacactc
      481 tcttaagtac ttttccgtat taaaaatagg tacaataatg ctgacttttt ccatcctcta
      541 attcctttta tgccgtaact tgctttgcta tcataaaaat catcatgtaa aaaatactat
      601 cttctatctt gccacctata gaacatatgc tctaccaaat taatagcaac tcaaaactct
      661 attcctgcca atttatgttt gcagttgttt gttaatagtt gattccat
//
LOCUS       DAZQCJ010000213          694 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000213 DAZQCJ010000000
VERSION     DAZQCJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.217"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            287..630
                     /gene="tnpA"
                     /locus_tag="ACHU66_002173"
                     /pseudo
     CDS             287..630
                     /gene="tnpA"
                     /locus_tag="ACHU66_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          234 a          113 c          144 g          203 t
ORIGIN      
        1 aacgaattag ttttttaagt gaatcagttc ctatagttgc tataacctgc tatagataat
       61 cctttaatag tttgagacta gaatggtagt attctagata gaaataagca acgaggttcc
      121 cccgttgctt attgtattta tgtgactttt agtccgtcgc gctccgtaag gtgcgtgaca
      181 aataaaccac ccgctatgcg ggtgcgcgac gaaggttata ccaaaaaact ctaaacgcga
      241 tacaataaag gtgttcaagc caattgtaaa gcgagaggag aaaagtatgg cacaaaaggc
      301 acatagttta tcacatacaa agtggttgtg ttctaccaca ttgtcttcac acctaagtat
      361 agacgaaaaa tcatctataa tcaatatcga agtagtttgg gagaaatatt ccgacatttg
      421 tgtagttata aaggcgtaga aattatcgaa ggacatctga tgccggatca tgttcatatg
      481 ttagtcagta ttcctccgcg attaagtgta tcaagcttta tgggatattt aaaaggcaag
      541 agtgctctta tgatgtttga taaacacgct aatctcaaat ataaatttgg caatcgtcat
      601 ttctggacag agggcttcaa caatttgggg aattgttgaa gagtgtaagt tggacttaat
      661 gaagccacaa ttaagaaata tatacaagaa caaa
//
LOCUS       DAZQCJ010000214          662 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000214 DAZQCJ010000000
VERSION     DAZQCJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.218"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            1..662
                     /locus_tag="ACHU66_002174"
                     /pseudo
     CDS             1..662
                     /locus_tag="ACHU66_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935295.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          197 a          152 c          138 g          175 t
ORIGIN      
        1 tttgggattg atgtgagtaa agtaagttca gaagtagcca ttctagtcaa cggcgagaag
       61 gttcataact acaccatgtc caatgatgcc attggctttt ctcggctact tggcgatttg
      121 agaaccgtcc ataagccaga aatcatcttt gaagcaacag gcgtctattc tcgccgtctc
      181 caaacttttc tagaagataa tggctatgct tatacacggc ttaatccctt agaagctaag
      241 aagcaactgg atagcttgcg tgtgcggaaa acagatcaaa ttgacgccga aaaactggct
      301 caatctcaat ttgtgctgaa tcgtaaaccc acttatgtcc aagaagaagt ctaccaaaac
      361 ttgcgggatc tcagccgttt ctatcagaat ctgaccgagg acattgttcg agccaaaaac
      421 cgtctgcaca aggtcttaca agtcactttt cctgaattgg aaaatatctt atcaacacca
      481 tctggcgaac aatactgaaa cctggtcata gcttttcctt gcaaggactt cgtgcttgat
      541 ttaagcaagg acgaactctc agagagcatt cgtcaatcca cttcaaaacg gatttcggac
      601 aagcgtgcag cgtacttagc cgagaagctg atagcactag ctaatcaatc gtattgtgcc
      661 gt
//
LOCUS       DAZQCJ010000215          641 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000215 DAZQCJ010000000
VERSION     DAZQCJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.219"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(141..>641)
                     /locus_tag="ACHU66_002175"
     CDS             complement(141..>641)
                     /locus_tag="ACHU66_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HFR3390405.1"
                     /translation="DGEKMLVEASDAGKYKDNIHFYNNAYVGVEVPNDPRAITEGIEL
                     NGIGTGPIDNKSMIANTGKYLTGQLDGYKIPFNSVLANSGVSKEDAITYFYEKLGIQQ
                     QIDFIENGSLNMSPIEAFSLARQTNSIDAIARSYPKIDGVNYDIDFFGESILTDNLSI
                     GAAQVK"
BASE COUNT          207 a          118 c           82 g          234 t
ORIGIN      
        1 aaatttatat tacaatttat tgagaatcag actgtaattt tgattttcac tttaaaaatt
       61 cttagactaa gtttcttcat tgtgttttaa cgaaaaaaat cattctattt gacttaatga
      121 aaaataacta gctgtttcac tcatttaact tgagcagcac ctatagataa attgtctgtt
      181 aaaatcgatt ctccaaagaa gtcaatatca tagttaactc catcaatttt tggatatgag
      241 cgagcgattg catctatact attcgtttga cgagctaaag aaaaggcttc tattggggac
      301 atatttaacg acccattttc aataaaatca atttgttgct gaatccctaa tttttcatag
      361 aaataagtaa ttgcatcttc ttttgatacg ccactatttg ccaatacact gttgaaagga
      421 attttatacc cgtctaattg accagttaaa tatttcccgg tatttgcgat cattgattta
      481 ttatcaattg gtcccgtacc aataccattt aattctatcc cttcagtgat tgctctagga
      541 tcattaggca cctcgacacc aacataggca ttattataaa aatgaatatt atctttatat
      601 ttacctgcat cacttgcttc aacaagcatt ttctcaccat c
//
LOCUS       DAZQCJ010000216          602 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000216 DAZQCJ010000000
VERSION     DAZQCJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.220"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(68..565)
                     /locus_tag="ACHU66_002176"
     CDS             complement(68..565)
                     /locus_tag="ACHU66_002176"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390406.1"
                     /translation="MILLTLYGMLILAAFWLWLFIARTSVKLACFAIDFCAVFIYTIY
                     LLHQPISSKIANGNTIYFWDVLFGLIAVLIYGFLMVVLSILLPRISKAINFVIVFLGV
                     GIGICLATDFITFLLSIFHSSIEPTYRIQFLTNNLANDILYYVIFFLVSIPVWKKRME
                     YLTGL"
BASE COUNT          250 a          108 c           76 g          168 t
ORIGIN      
        1 aactttatca acacaagaaa aattttgact cttactttga aacagagcaa aatcgaatca
       61 tttaattcta tagcccagtt aaatactcca ttcgcttttt ccagacagga attgaaacta
      121 agaaaaagat tacatagtat aaaatatcat tggcaagatt attagtcaaa aattgtatgc
      181 gataggtcgg ctcaatactc gaatgaaaga ttgataataa gaaagttatg aaatcagttg
      241 ctaaacaaat tcctatacca acacccaaaa atacaatgac aaaattgatg gctttactta
      301 ttcttggcaa taatattgag agaacaacca ttagaaaacc atatatcaaa actgcgatca
      361 atccaaataa aacatcccaa aaataaatcg tatttccatt cgcaatctta ctagaaattg
      421 gttggtgtaa cagataaatt gtgtagataa atactgcaca aaaatcaatc gcaaaacagg
      481 caagttttac agaagttcta gctataaata accaaagcca aaatgctgct aagattaaca
      541 ttccatacag tgttaacaaa atcattaaaa actcactcct ttcactacat ctagtttact
      601 gc
//
LOCUS       DAZQCJ010000217          588 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000217 DAZQCJ010000000
VERSION     DAZQCJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.221"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..>588
                     /locus_tag="ACHU66_002177"
     CDS             <1..>588
                     /locus_tag="ACHU66_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390407.1"
                     /translation="DNNLVSLNYLIDQLNNLEDFSMSLNGEVPSEISELKSSVFNFSQ
                     ESALFMENFSQQGWCLYESMDFDIVQRVNSEYEKNGIDKAERILIDYFKNEVRKKVHW
                     IKKSSKVLLDRSHLIDVFFEEHFKGNYICSVPLGLIIVDGAVNDFTKNKDNKGLFAEG
                     LELNVWDSFVGSNKSLKEIVSIFTSSRRKTNKDEI"
BASE COUNT          218 a           56 c          113 g          201 t
ORIGIN      
        1 tggataataa tttggtatca ttaaattatt taatagatca acttaataat ttggaagatt
       61 tttctatgtc attgaatgga gaagtgccgt cagaaatttc tgagttaaaa agttcggttt
      121 ttaatttttc tcaagaatct gccctattta tggaaaattt ttctcaacaa ggttggtgtc
      181 tctatgaaag tatggacttt gatatagtgc agagagtaaa ctcggaatat gaaaaaaatg
      241 gtattgataa agcagaaaga atactgatag attacttcaa aaatgaagta agaaaaaaag
      301 tgcattggat taaaaaaagt tccaaagttc ttttagatag gtctcatttg atagatgtat
      361 tttttgagga acattttaaa ggcaattata tttgtagcgt gccacttggt ttaatcattg
      421 ttgacggtgc agttaatgat tttacaaaaa ataaagataa caaaggtttg tttgcagaag
      481 gtttagaact aaatgtatgg gattcatttg tgggtagtaa taagtcgtta aaagaaatag
      541 tatcaatttt tacatctagt cgacgaaaaa cgaataaaga tgaaatta
//
LOCUS       DAZQCJ010000218          540 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000218 DAZQCJ010000000
VERSION     DAZQCJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.222"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..>540
                     /locus_tag="ACHU66_002178"
     CDS             <1..>540
                     /locus_tag="ACHU66_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HFR3390408.1"
                     /translation="SHLVEKESTTHISDIVVPVFAKDEIKEPRYIKEILPEVIPTNVR
                     QKIPFDEWLYFKLYMSADRQEEFLSDIMPQILKLVNLDDGMSFYIRYTDPKFHIRLRI
                     RTQNLYKSFEKIQEIFQLCIQNKLISNIDISTYDREIERYGGLERIPLVEEIFCLDTE
                     IVINSLSLIRQKRLDLTLD"
BASE COUNT          206 a           71 c           96 g          167 t
ORIGIN      
        1 attcacacct tgtagagaag gaatctacaa ctcatatttc tgatatagtt gttccagtat
       61 ttgctaagga tgaaattaag gaacctaggt acataaaaga aatattacca gaggttattc
      121 cgacaaatgt tagacagaaa ataccatttg atgaatggct ctactttaaa ttgtatatgt
      181 cggcagatag gcaagaggaa tttctttcgg atataatgcc tcaaattttg aagttagtaa
      241 atttagatga tgggatgagc ttctatatca gatatactga tccaaaattt catatccgac
      301 taaggataag aacgcagaat ttatataaaa gttttgaaaa aattcaagaa atttttcaac
      361 tctgcataca aaacaagtta atatcaaata tagatatttc aacgtatgac agagaaatcg
      421 aaaggtatgg gggactagaa agaattcctt tggtagaaga aattttttgt ctagatactg
      481 aaatagtcat caattcatta agtttaattc ggcagaaaag attagattta accctagatg
//
LOCUS       DAZQCJ010000219          540 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000219 DAZQCJ010000000
VERSION     DAZQCJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.223"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(187..>540)
                     /locus_tag="ACHU66_002179"
     CDS             complement(187..>540)
                     /locus_tag="ACHU66_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR3390409.1"
                     /translation="ILTEMYNVRGLLTDNHQIKFLKVNYRLIITPDFAKFLPHEFIVV
                     PDTLDIEQVKSQYVGTGVDLSKIISLKEYRKEIGFIGNLYALLGFVPNMLNRIYLYIQ
                     RNGIANTIIKIKSRL"
BASE COUNT          192 a           99 c           66 g          183 t
ORIGIN      
        1 taagatagat actgaatcac agaagctttg cctatcgcta gaacaacttg cgaattacta
       61 ataattatgc ccataaacgc tatggcaaca aaaaacaaat tattaattct tattctcatt
      121 tttccaaacg tttttctcct atctgcctgc attactttta aaattacaaa taaaagtaaa
      181 caactctcac aatctcgact tgatttttat aatagtgttt gcaataccgt ttctctgaat
      241 atatagataa attctattga gcatattcgg aacaaatcct aaaagcgcat acaaattacc
      301 aataaagcct atctcttttc gatactcttt taaagaaata atctttgaca agtctacacc
      361 tgtaccaaca tactggcttt taacttgctc tatatccaag gtatctggta caacaataaa
      421 ttcatgcggt aaaaacttag caaaatctgg agttataatt aatctataat taactttgag
      481 aaatttaatt tggtgattat cggtaagtaa gcctctgacg ttatacattt cggttaagat
//
LOCUS       DAZQCJ010000220          533 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000220 DAZQCJ010000000
VERSION     DAZQCJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.224"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            26..533
                     /locus_tag="ACHU66_002180"
                     /pseudo
     CDS             26..533
                     /locus_tag="ACHU66_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          197 a           94 c          116 g          126 t
ORIGIN      
        1 atctaacatt tggggtgcta tttttatgaa attgagctat gaagacaaaa tcaaaatcta
       61 tgaactacgt caaagtggtc agtctatcaa gaacttgtca aaccaattta ctatttcaga
      121 atctgttatc caatatatgc ttcgattgat tgataggtat ggaataaaca ttgtcaagaa
      181 aggtaagaac acttattctc ctccagaact caagcaggaa atgattgata aagtacttct
      241 tcaaaagcag tcgagtctgt cagtctcact tgattacgcc ttgccaaacc gaggaactct
      301 cccaaactgg atagcacaat acaagaaaaa cgggtatact attcttgaga aaacaagagg
      361 gaggccacca aagatggggc gtaaaccaaa gaaaacctgg gaagagatga cggagttaga
      421 gcgacttcag gaggaaaatg aacggcttag aacggagaat gtctatctaa aaaagttgag
      481 agcgcttcgt ttaagggacg aagccagaga acaagaaagg ctcaaacagt tag
//
LOCUS       DAZQCJ010000221          518 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000221 DAZQCJ010000000
VERSION     DAZQCJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.225"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..>518
                     /locus_tag="ACHU66_002181"
     CDS             <1..>518
                     /locus_tag="ACHU66_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISSsu6 family transposase"
                     /protein_id="HFR3390410.1"
                     /translation="AMTHIAHRLSISTSSVMRKLNEFKFETDWNTLPEVMSWDEYSFK
                     KGKMSFIAQDFNSLNVITILDGRTQATIRNHFLRYPRKVRNQVKVITMDMFSPYYQLA
                     KQLFPNAKIVLDRFHIVQHLSRAMNRIRIQIMNQFDRTSQEYRALKRYWKLIQQDSRK
                     LSDKRFYRPMFR"
BASE COUNT          172 a          108 c           92 g          146 t
ORIGIN      
        1 gcaatgacac atattgcaca caggctatct atctcaacct catcagttat gagaaagctt
       61 aatgagttca agtttgaaac agattggaat accttacctg aggtgatgag ctgggatgaa
      121 tatagcttca agaaggggaa aatgagcttt atcgctcaag atttcaactc cctaaatgtc
      181 attactattc tggacggaag aacacaagca accatccgaa accactttct acgctatcct
      241 agaaaggtta gaaatcaagt caaagtcatt accatggata tgtttagtcc ctattatcaa
      301 cttgctaaac agctttttcc gaatgcaaaa attgtactcg accgctttca tattgttcaa
      361 caccttagtc gtgctatgaa ccgtattcgt attcaaatta tgaatcaatt cgacagaaca
      421 tcgcaggaat acagagcctt gaaacgctac tggaaattga ttcaacagga tagtcgtaaa
      481 ctcagcgata aacgatttta tcgccctatg tttcgaat
//
LOCUS       DAZQCJ010000222          506 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000222 DAZQCJ010000000
VERSION     DAZQCJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.226"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..>506
                     /locus_tag="ACHU66_002182"
     CDS             <1..>506
                     /locus_tag="ACHU66_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFR3390411.1"
                     /translation="EQDPEKVELFLKELNNLSHLTPVYIDETGFETCFHREYGRSLKG
                     QLIKGKVSGRRYQRISLVAGLINGALIAPMTYKDTMTSGFFEAWFKTFLLPTLGKPSV
                     IIMDNAKFHRMSKLKDLCEEQGHRLLPLPPYSPEYNPIEKIWAHIKKHIRRVLTNCDT
                     FLEVLSSC"
BASE COUNT          163 a           96 c          103 g          144 t
ORIGIN      
        1 atgaacaaga ccctgaaaaa gtagaactgt tccttaaaga attgaataac ttaagccact
       61 tgactcctgt ttatattgac gagacagggt ttgagacatg ttttcatcga gaatatggtc
      121 gctctttgaa aggtcagttg ataaaaggta aggtctctgg aagaagatac cagcggatat
      181 ctttagtggc aggtctcata aatggtgcgc ttatagcccc gatgacatac aaagatacta
      241 tgacgagtgg ctttttcgaa gcttggttca aaacattctt actacccact ttaggtaaac
      301 catctgttat catcatggac aatgcaaagt ttcataggat gagtaagcta aaagatttat
      361 gcgaggagca gggacataga cttttaccac ttcctcctta ctcaccggaa tataatccca
      421 ttgagaaaat atgggctcac atcaaaaaac acatcagaag agtattgaca aattgcgata
      481 cttttcttga ggtactttcg tcctgc
//
LOCUS       DAZQCJ010000223          505 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000223 DAZQCJ010000000
VERSION     DAZQCJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.227"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..>505)
                     /locus_tag="ACHU66_002183"
     CDS             complement(<1..>505)
                     /locus_tag="ACHU66_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFR3390412.1"
                     /translation="LISIGLIGSFLLYTHLIQNELGSTKNDGTVEIIAEQPNQVIDSI
                     QINGRYIHSSEIIENQWGINDADLIPNFSSLGEENSLVIKSSSSVRTLSFEYFVSENP
                     TIVKIKVGGQLVESIDTSTGDKYKNLAFIELPYTLRITKDNQFWYLHLIVLALGLTCF
                     ILNGATWR"
BASE COUNT          159 a           78 c           88 g          180 t
ORIGIN      
        1 tctccatgtt gctccattta agataaagca tgttagacct aaagcaagga ctattaagtg
       61 gaggtaccaa aattgattat ctttagttat ccgtaaagta tagggaagct caataaaggc
      121 taaattttta tatttatctc cagttgatgt gtcaatggat tcaaccaatt gtccaccgac
      181 tttaattttt acaatagtcg gattttcact aacaaagtat tcaaatgata aggttcttac
      241 agaagaggaa gatttaatta cgagtgaatt ttcctcacct agagaagaga aattagggat
      301 taaatcagca tcattgattc cccattggtt ttctataatt tctgatgaat gaatatagcg
      361 tccatttatt tggatgctgt cgattacttg gtttggttgt tctgctataa tttctacagt
      421 tccatcattt ttcgtagaac ctaattcgtt ttgtattaga tgtgtataca gtaaaaaact
      481 tccaatcaat ccgatggaga ttagt
//
LOCUS       DAZQCJ010000224          460 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000224 DAZQCJ010000000
VERSION     DAZQCJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.228"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
BASE COUNT          174 a          100 c           54 g          132 t
ORIGIN      
        1 taaaaactgc ggaaattatc aaggataaca acaatattgg taacacaatc ccatcctgtg
       61 ttgccaatgc tgctcctaga aaatctaaaa aattatccgt atattcagcc tttacgccta
      121 ccaaaaattt ggacagtata ggaactaaga ttggtaaacc agcaaaagca tagaacaaag
      181 caacatccct tcgatgtatt accgatttaa aaattgacca aaacatattc atattgtttc
      241 ccctttttat caataagata gatgagcaat aactccctca cttatttatt cgtaaacaaa
      301 aagaaaaatt ctctggaaaa accaaagaat tttaatactc atatttcacc atcaattatg
      361 gtctcaaata atgtgccgcc acataaccaa caacgaaacc tgtaactgca gctactagca
      421 tgtgtttctc ctccttcata atataatact ctttcttcaa
//
LOCUS       DAZQCJ010000225          451 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000225 DAZQCJ010000000
VERSION     DAZQCJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.229"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..392)
                     /locus_tag="ACHU66_002184"
     CDS             complement(<1..392)
                     /locus_tag="ACHU66_002184"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HFR3390413.1"
                     /translation="MMIISVLATLPISLIKDIDDFRRISNHLLFALFITSILGIMMGA
                     TMFTGAVEGIGFSQGFNGGLTHKNFFGITILMGFVLTYLAYKYGSYKRTDRFILGLEL
                     FLILISNTRSVYLILLLFLFLVNLDKIK"
BASE COUNT          176 a           80 c           57 g          138 t
ORIGIN      
        1 attttgattt tgtcaagatt aacaagaaat agaaaaagca atagtattaa ataaactgag
       61 cgtgtgtttg aaataagaat caaaaacaat tctaatccta aaataaaacg atccgttctt
      121 ttataggaac catacttata cgccaagtaa gttaatacga accccattaa aatagttatt
      181 ccaaaaaagt tcttatgcgt caatcctcca ttaaaaccct gactaaaacc gataccttct
      241 actgcccccg tgaacatcgt tgcccccatc attattccta atatcgaagt tataaaaaga
      301 gcgaataaca aatgatttga aatccgtcta aaatcatcaa tatcttttat caaacttatt
      361 ggcaacgttg ctaaaactga aataatcatc attgaaattg ataaatatat tttagttgtt
      421 ataggaagaa tttgctgaca tattccgata g
//
LOCUS       DAZQCJ010000226          428 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000226 DAZQCJ010000000
VERSION     DAZQCJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.230"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(11..172)
                     /locus_tag="ACHU66_002185"
     CDS             complement(11..172)
                     /locus_tag="ACHU66_002185"
                     /inference="COORDINATES: protein motif:HMM:NF025153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HFR3390414.1"
                     /translation="MVTPEETYFFNQENIVAIVFRGYVNIDEQSFSSQCDTLISESGY
                     EKGKVLSYN"
BASE COUNT          145 a          100 c           44 g          139 t
ORIGIN      
        1 attcaaatta ctaattatat gatagaactt ttcctttttc ataaccagat tctgaaatca
       61 atgtatcaca ctgacttgaa aaagattgct catcaatgtt gacataacca cgaaatacta
      121 tagctacaat gttttcttga ttgaaaaagt aagtttcctc tggagtaacc aatccctgtg
      181 ggattcgaac tgcccataat caaaataaac ttcttttcca tcctgaagaa caataaaacc
      241 cctattgata ataattaagt ctacattttc acttctattt agccttacta cactacccat
      301 ccgataatat atcgctattc tttacaaaat actcttcaaa cttataaaca ctgtctgcaa
      361 tatcggcaga tacctccacc cgacgattga tatcagccct actcatctgt tttatcctcc
      421 atcacaaa
//
LOCUS       DAZQCJ010000227          424 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000227 DAZQCJ010000000
VERSION     DAZQCJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.231"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..>424
                     /locus_tag="ACHU66_002186"
     CDS             <1..>424
                     /locus_tag="ACHU66_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR3390415.1"
                     /translation="EIFKFVSDKGYADLPILSASQELGMIRRDEIGIDIKYDKEAVAN
                     YKRVLPGQFVIHLRSFQGGFAWSEIEGLTSPAYTILDFKEENSSKFWRNVLTSPNFIK
                     KLETVTYGIRDGRSISYSDFSTLNFVIPTLPEQEAIGS"
BASE COUNT          137 a           68 c           93 g          126 t
ORIGIN      
        1 tggagatttt taagtttgtg tctgacaaag ggtatgcaga tttaccaatt ttgtcagctt
       61 ctcaagaact tgggatgatt agacgagatg aaataggaat tgacatcaaa tatgacaagg
      121 aagcggtagc gaattataag cgtgttttac cgggtcaatt tgtaattcat cttcgatcat
      181 ttcaaggagg atttgcatgg tctgagatag agggactgac atcacctgcc tatacaatat
      241 tggattttaa ggaagaaaac tcgtcaaaat tttggcgaaa tgtgttaact agtccgaatt
      301 tcataaaaaa acttgaaaca gttacatatg gaataaggga tggacggagc ataagttatt
      361 ctgatttttc gacattaaat tttgtcatcc ccacgctccc cgaacaagaa gccatcggca
      421 gctt
//
LOCUS       DAZQCJ010000228          422 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000228 DAZQCJ010000000
VERSION     DAZQCJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.232"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            3..73
                     /locus_tag="ACHU66_002187"
     tRNA            3..73
                     /locus_tag="ACHU66_002187"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Gly,seq:tcc)
     gene            104..177
                     /locus_tag="ACHU66_002188"
     tRNA            104..177
                     /locus_tag="ACHU66_002188"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:138..140,aa:Ile,seq:gat)
     gene            184..255
                     /locus_tag="ACHU66_002189"
     tRNA            184..255
                     /locus_tag="ACHU66_002189"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:217..219,aa:Glu,seq:ttc)
     gene            264..353
                     /locus_tag="ACHU66_002190"
     tRNA            264..353
                     /locus_tag="ACHU66_002190"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Ser,seq:tga)
BASE COUNT           95 a          101 c          119 g          107 t
ORIGIN      
        1 aagcgggtgt agtttagtgg taaaactaca gccttccaag ctgttgtcgc gagttcgatt
       61 ctcgtcaccc gctttgaaca tagttcatac ccaaacttgg tttgggcgcg tagctcagat
      121 ggttagagcg cacgcctgat aagcgtgagg tcggtggttc gattccactc gtgcccatta
      181 tatggtccgt tggtcaaggg gttaagacac cgccttttca cggcggtaac acgggttcga
      241 atcccgtacg gactatatta ttcggaggat tacccaagtc cggctgaagg gaacggtctt
      301 gaaaaccgtc aggcgtgtaa aagcgtgcgt gggttcgaat cccacatcct ccttactatt
      361 atcgcgggat ggagcagcta ggtagctcgt cgggctcata acccgaaggt cgtaggttca
      421 aa
//
LOCUS       DAZQCJ010000229          421 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000229 DAZQCJ010000000
VERSION     DAZQCJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.233"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..>421
                     /locus_tag="ACHU66_002191"
     CDS             <1..>421
                     /locus_tag="ACHU66_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390416.1"
                     /translation="KKIIRKSSSKRLKSEVLSQAYQDYITTGQVSNIPHINLEELKNN
                     SNLLLKEHSKVKKEKQEYDKLISEQISQIVTLKKPLLELILPHKNFHPFELGHREIRY
                     YAAPIPIENTVYIDFISNTYGKSKSSFFSDFAYVVSVR"
BASE COUNT          167 a           65 c           60 g          129 t
ORIGIN      
        1 aaaaaaaatt attcgtaagt ctagctccaa acgtttgaaa tcggaagtat tatcacaagc
       61 ttatcaagac tatattacaa ctggtcaagt tagtaatata cctcatatta atttggagga
      121 attaaaaaat aatagtaatt tgttgctaaa agaacactcc aaagtaaaaa aagaaaaaca
      181 agaatatgat aaattgataa gcgagcagat ttctcaaatt gttacgttaa aaaaaccatt
      241 gcttgaactt attttacccc acaaaaactt tcatccattc gaactaggtc atcgagaaat
      301 aagatactat gcggcgccaa ttcctatcga aaacacggta tatatagatt ttatttctaa
      361 tacatatggt aaaagtaaaa gctcattctt ttctgatttt gcttatgtcg tatcggtaag
      421 a
//
LOCUS       DAZQCJ010000230          414 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000230 DAZQCJ010000000
VERSION     DAZQCJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.234"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            94..408
                     /locus_tag="ACHU66_002192"
     CDS             94..408
                     /locus_tag="ACHU66_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SSU0592/SSU0593 family protein"
                     /protein_id="HFR3390417.1"
                     /translation="MKQLVTKRSYKIGLSAMALVLLMTTAGGATAHASTGTYSVETRG
                     DAEVYRLLETPAGSFTQGNVVLEPVNNSTTKPSETKSWFDSFWSSVKTFWEAICRGVG
                     WC"
BASE COUNT          121 a           63 c          100 g          130 t
ORIGIN      
        1 ctaacctttt tctaaagttg gaatttgagt tgtctgtttg tgctatacta tcatttgtag
       61 agacgaatat cgtataaagg agatatttca acaatgaaac aacttgtaac aaaacgtagt
      121 tataaaattg gtttgagtgc tatggcttta gtcttgctaa tgaccactgc aggtggtgcc
      181 actgcacatg caagtacggg tacctatagt gtcgaaactc gaggtgatgc cgaagtgtat
      241 aggctgttgg aaactccggc ggggtctttt acacagggga acgtagtttt ggaacctgtg
      301 aataattcta caactaaacc atctgaaacc aaatcatggt ttgattcttt ttggagtagt
      361 gtaaaaacat tttgggaagc aatctgtcgt ggagttggat ggtgttaagt agaa
//
LOCUS       DAZQCJ010000231          411 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000231 DAZQCJ010000000
VERSION     DAZQCJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.235"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..>411)
                     /locus_tag="ACHU66_002193"
     CDS             complement(<1..>411)
                     /locus_tag="ACHU66_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HFR3390418.1"
                     /translation="IMKRRGISYLDDAESDNEAGRSDYAKAIERNRQLLTSKTPGEIQ
                     LERLEKYGQLRGNFTIIDEEGQSQQIINVFSTSDYVKEVEKILDCQKMYHKFISDEFC
                     DKLIELLREKRKYYVGPGNEKSRTDYGIYRTDGTT"
BASE COUNT          112 a           97 c           50 g          152 t
ORIGIN      
        1 gttgtcccat ctgttctata aataccatag tcagttctag atttctcatt ccctggacca
       61 acataatact ttcttttttc tcttaatagt tcgatcagct tatcgcaaaa ttcatcagat
      121 ataaatttat gatacatttt ttgacaatct aaaattttct caacttcctt gacataatct
      181 gatgttgaaa aaacattgat aatttgttga gattgcccct cctcatcaat tatcgtgaaa
      241 tttccacgca actgaccgta tttctctaag cgctctaact gaatttcccc tggtgtttta
      301 ctagtcaata actgtctatt acgctcgata gctttagcat aatcacttct tcccgcctcg
      361 ttatctgact ctgcatcatc tagataggat atccccctgc gcttcataat a
//
LOCUS       DAZQCJ010000232          400 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000232 DAZQCJ010000000
VERSION     DAZQCJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.236"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
BASE COUNT          129 a           67 c           85 g          119 t
ORIGIN      
        1 atctttaatt actttaatgt atggatatcc ttcccactcc cgtcaattat agagatcagt
       61 ttcagactgt aaaacacgaa ttgctaagat gttgacaaaa gttggcggcg gcagctcatt
      121 gccttgtgtg gtcgaatgaa ttagctttca gacaaggaac tgaggtgcag gtagctagaa
      181 cagcctagag gctgttctag gttggaaata agaattgcga aacaagtcac tttcgtagag
      241 tacgacaagc cgaaagcggc gatgtatcag agttaattta aatgactgta ctaagaactt
      301 gctattttgg ggatttcatt gagtattaaa atttatctac ataaaaacac cttgaaaatg
      361 cgcttgtcat ttattataaa taacgagagc ttcttcaaga
//
LOCUS       DAZQCJ010000233          393 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000233 DAZQCJ010000000
VERSION     DAZQCJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.237"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..352)
                     /locus_tag="ACHU66_002194"
     CDS             complement(<1..352)
                     /locus_tag="ACHU66_002194"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFR3390419.1"
                     /translation="MAIGAYLFHPESSPEQFLFIHIGPGLFCSFFDSEHILQNKNFYI
                     GEIGHTVVDLNGPSCECGKRGCLQTYISDTWLINNARFLFENVQGSIIKTLVEKPEDI
                     TLDVVYNAYRLGDGF"
BASE COUNT          147 a           68 c           53 g          125 t
ORIGIN      
        1 taaaaccatc tcctagtctg tatgcattgt atacaacatc taatgtaata tcttctggtt
       61 tttcaaccaa agtttttatg atagaacctt gaacattttc aaataaaaat cttgcattat
      121 taattaacca agtatcagaa atataagttt gaaggcatcc gcgttttcca cattcacaag
      181 aaggtccgtt taaatcaact actgtatgac caatctctcc aatataaaag tttttgtttt
      241 gtaaaatatg ttcgctatca aaaaatgaac agaacaatcc aggtccaata tgaataaata
      301 aaaactgctc tggcgaactt tcgggatgaa aaagatatgc accaattgcc atgcaattaa
      361 tattgttctc tagaaaaata ggaattgaaa tac
//
LOCUS       DAZQCJ010000234          370 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000234 DAZQCJ010000000
VERSION     DAZQCJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.238"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..368)
                     /locus_tag="ACHU66_002195"
     CDS             complement(<1..368)
                     /locus_tag="ACHU66_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR3390420.1"
                     /translation="MAQSNKNVDILLFGKGIAFGKKVGDAIPPSSIEKSFLLKNSDNM
                     NRFTELFIDVPIELVYVCEKIINLGKITLGNHFDEIIYINLTDHINSSIERHKEGVVV
                     TNPLRWEIAKYYKEEFELGK"
BASE COUNT          116 a           77 c           42 g          135 t
ORIGIN      
        1 tttttcccca gttcaaactc ctctttataa tatttggcaa tttcccatct aagagggttc
       61 gtaacgacta ctccttcctt atgtctttct atactagaat tgatatgatc tgtcaaattg
      121 atgtaaataa tctcatcaaa atgatttcca agtgttatct ttcctaaatt tattattttc
      181 tcacaaacat aaaccaactc aataggaaca tcgataaata attccgtaaa gcgattcata
      241 ttatcgctat tcttgagcaa aaaacttttt tctatcgaag aaggtgggat tgcatctccg
      301 accttctttc caaatgcaat tcctttacca aacaacaata tatcaacgtt cttattactt
      361 tgtgctatga
//
LOCUS       DAZQCJ010000235          361 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000235 DAZQCJ010000000
VERSION     DAZQCJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.239"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            30..>361
                     /locus_tag="ACHU66_002196"
     CDS             30..>361
                     /locus_tag="ACHU66_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFR3390421.1"
                     /translation="MEQLNLITNFLRIKDKNIVITDEYDMGTHLELHGHLDYTAPKCP
                     SCKGQMTKYDFQKASKIPYLETAGYPLLIRLRKRRFKCKDCGKIAVAETPLVKKNHQI
                     SVAVNQKIA"
BASE COUNT          130 a           86 c           57 g           88 t
ORIGIN      
        1 cgactaaaca actcattaga aagactcata tggaacaact aaatcttatc acaaattttc
       61 tcagaattaa agataaaaat atcgttatca ctgatgaata tgatatggga actcacttag
      121 aactccacgg tcacttggat tacacagccc ctaaatgccc ttcctgcaag ggacaaatga
      181 caaaatacga tttccaaaaa gcctccaaaa ttccctacct agaaacagct ggctatccct
      241 tacttatccg acttagaaag cgtcgcttca agtgtaaaga ttgcggaaaa atagcggttg
      301 ctgaaactcc tcttgtcaag aagaaccacc aaatctctgt cgcggttaac cagaaaatcg
      361 c
//
LOCUS       DAZQCJ010000236          361 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000236 DAZQCJ010000000
VERSION     DAZQCJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.240"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            30..>361
                     /locus_tag="ACHU66_002197"
     CDS             30..>361
                     /locus_tag="ACHU66_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFR3390422.1"
                     /translation="MEQLNLITNFLRIKDKNITITDEYDMGTHLELHGYLDYMAPKCP
                     ECKGQMAKYDYQKASKIPYLETAGYPLLIRLKKRRFKCKECGKMAVAETPLVKKNHQI
                     SVAVSQKIA"
BASE COUNT          128 a           76 c           62 g           95 t
ORIGIN      
        1 gctcaaacta tcattttaga aagactcata tggaacaact aaatcttatc acaaattttc
       61 tcagaattaa agacaaaaat atcactatca ctgatgaata tgatatggga actcacttag
      121 aactccacgg ttacttggat tacatggctc ctaaatgtcc agaatgcaag ggacaaatgg
      181 ctaagtacga ctaccagaaa gcatcaaaaa ttccctacct agaaactgct ggttatccac
      241 tccttatccg ccttaaaaag cgtcgtttca agtgtaaaga atgtgggaaa atggcggttg
      301 ctgaaactcc tcttgttaag aaaaaccatc aaatatctgt cgctgttagc cagaagatcg
      361 c
//
LOCUS       DAZQCJ010000237          347 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000237 DAZQCJ010000000
VERSION     DAZQCJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.243"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..>347
                     /locus_tag="ACHU66_002198"
     CDS             <1..>347
                     /locus_tag="ACHU66_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR3390423.1"
                     /translation="PSTYHYQVKQLDKPDKDKDLKAEIQAIYDENKGNYGYRRIHLEL
                     RNRGFSVNHKKVQRLMKELGLTARIRRRKRYKSYKGDVGKKAPNLIERAFEGAKPFEK
                     CYTDVTEFALPNCA"
BASE COUNT          115 a           65 c           78 g           89 t
ORIGIN      
        1 ccttctacct atcattatca agtcaaacaa ctggacaaac cagataaaga taaggactta
       61 aaagctgaaa ttcaagccat ttatgatgaa aataagggaa attatggcta ccgtcgcatt
      121 caccttgagc taagaaatcg tggattttct gtcaaccaca agaaagtaca acggctgatg
      181 aaagagttag gtttgactgc tcgtattcgt agacgcaaga gatacaagtc ttacaagggt
      241 gatgtgggca agaaagctcc taatctcatt gagagagcct ttgaaggggc taagcctttt
      301 gagaagtgct atacagatgt gactgagttc gctctgccaa attgtgc
//
LOCUS       DAZQCJ010000238          337 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000238 DAZQCJ010000000
VERSION     DAZQCJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.244"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            <1..>337
                     /locus_tag="ACHU66_002199"
     CDS             <1..>337
                     /locus_tag="ACHU66_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR3390424.1"
                     /translation="SIFLHEQEEKANFFQNIKSEKNSGIIWGIKLISTDLLMVLLGVP
                     VWIVVGVEFNRLSYFAYVGVITWLLLVLLNHFHMLLSLITGKGGNLVISFIECLFIIF
                     ATNKVFLNI"
BASE COUNT          104 a           40 c           61 g          132 t
ORIGIN      
        1 tctctatttt tcttcatgaa caagaggaaa aagcaaattt ctttcaaaat ataaaaagcg
       61 aaaaaaatag tggaattatt tggggaataa agcttatttc gacagactta cttatggttt
      121 tattgggagt tccagtttgg atagtggtag gagttgaatt taatagactc tcttactttg
      181 cgtatgttgg tgtgattact tggttattat tagtcttact taaccatttc catatgttac
      241 ttagtcttat cacgggaaag ggagggaatc tagtaatttc ttttatcgaa tgtctgttta
      301 ttatatttgc aaccaataaa gtatttttaa atatctt
//
LOCUS       DAZQCJ010000239          330 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000239 DAZQCJ010000000
VERSION     DAZQCJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.245"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..>330)
                     /locus_tag="ACHU66_002200"
     CDS             complement(<1..>330)
                     /locus_tag="ACHU66_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HFR3390425.1"
                     /translation="FLYYFFGFLITHSDSYAHRVNGLINFFEYYRNDWFHLMFGAADL
                     AYGDLTLDYAIRVRRVLGWNGTLEMPLLSIMLKNGFIGLVGYGIVLYKLYRNVRILKT
                     DNIKTIG"
BASE COUNT          129 a           67 c           35 g           99 t
ORIGIN      
        1 ttcctattgt ttttatatta tctgttttta atattcttac attacgataa agtttatata
       61 aaacaatccc ataccctacc agaccgataa aaccattttt taacatgata ctcagtaagg
      121 gcatttcaag cgttccattc caacctaaaa cgcgtctaac ccttatagca tagtctaaag
      181 ttaaatcccc atatgccaaa tccgctgcac caaacattag atggaaccaa tcatttctat
      241 aatactcaaa aaaattaata agaccattaa cgcgatgagc gtaagaatca ctatgtgtta
      301 ttaaaaaacc aaagaaatag tataaaaaaa
//
LOCUS       DAZQCJ010000240          287 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000240 DAZQCJ010000000
VERSION     DAZQCJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.249"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            24..>287
                     /locus_tag="ACHU66_002201"
     CDS             24..>287
                     /locus_tag="ACHU66_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390426.1"
                     /translation="MRKKLIATIAFCVVCLFLYSFVPQSFGTPTIFQTPYKTSVKSEF
                     EALFHKHGIDVSENDFWATSLNGRTPQDILNEKAKLLYIKFNLL"
BASE COUNT          100 a           46 c           40 g          101 t
ORIGIN      
        1 tatttggata aggaggtaac aatatgagaa aaaaattgat agccacgatt gcattttgcg
       61 tagtttgtct atttctctat tcatttgttc ctcaatcttt cggtactcca actatttttc
      121 agacacctta taaaacctct gtgaaatccg aatttgaagc attatttcat aaacatggta
      181 ttgatgtctc agaaaatgat ttttgggcta catcattaaa tggtcgaaca cctcaagaca
      241 ttctaaatga aaaagcaaaa ttactatata taaaattcaa tttatta
//
LOCUS       DAZQCJ010000241          267 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000241 DAZQCJ010000000
VERSION     DAZQCJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.denovo.251"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            43..>267
                     /locus_tag="ACHU66_002202"
     CDS             43..>267
                     /locus_tag="ACHU66_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR3390427.1"
                     /translation="MLSKYVGRQTSSIENDITKTRNHSRQRGNIMRVVFGIDVSKVSS
                     EVAILVNGEKVHNYTMSNDAIGFSRLLGDLK"
BASE COUNT           92 a           47 c           62 g           66 t
ORIGIN      
        1 gacgtcgcaa caaaaactgg caagacacct gttttagaaa gaatgttatc aaagtatgtg
       61 ggacgccaga cgtcgagtat tgaaaatgac atcactaaaa caaggaatca ttcaagacaa
      121 agaggtaata tcatgcgagt agtatttggg attgacgtga gtaaagtaag ttcagaagta
      181 gccattctag tcaacggcga gaaggttcat aactacacca tgtccaatga tgccattggc
      241 ttttctcggc tacttggcga tttgaaa
//
LOCUS       DAZQCJ010000242        15964 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.guided.040, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000242 DAZQCJ010000000
VERSION     DAZQCJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15964
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.guided.040"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(<1..269)
                     /locus_tag="ACHU66_002203"
     CDS             complement(<1..269)
                     /locus_tag="ACHU66_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFR3390428.1"
                     /translation="MSKKEILLDFIEKNNGIVTNKDCKALGIPTIYLTRLEKEGIIFR
                     VEKGIFLTQNGDYDEYYFFQYRFPKAIFSYISALYLQQFTDEIPQ"
     gene            548..5443
                     /locus_tag="ACHU66_002204"
     CDS             548..5443
                     /locus_tag="ACHU66_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0018262 - isopeptide cross-linking
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SspB-related isopeptide-forming adhesin"
                     /protein_id="HFR3390429.1"
                     /translation="MTKTCNHHFLVNQEKGEKHVFRKSKKYRTLCSVALGTMVTAVVA
                     WGGTVAHADEVTSSVDTNIQRTENPATNLPEAQPKPVSEQTEILALTGQSNGAIAVTV
                     PHDTVTQAVEEAKAEGVSTVEDSQMDLGNTTSAAETNQQISKAEADAQNQVEAINEVT
                     ETYKADKAAYVDEKARIEQENKELSQAYEGANQTGKETNTWVDTKVKDLKTRYADADV
                     TVKEQVVSSGNGTSVLDYTNYGKAVETIQSTNEQAVADYLTKKTKADDIVAKNQAIQK
                     ENEAGLAKAKADNEAIERRNQAGQAAVDAENRAGQAAVDQANQEKQQLVSDRAAEIEA
                     ITKRNQEKEAAARKENEVIDAYNTKEMERYQRDLAEISKGEEGYISEALAQALNLNNG
                     EPQAQHGAITRNPNQIISTGDAMLGGYSRILDSTGFFVYDSFKTGETLSFNYQNLQNA
                     RFDGKKISRVTYDITNLVSPAGTDAVKLVVPNDPTEGFIAYRNDGNGDWRTDKMEFRV
                     VAKYFLEDGTQVTFSKEKPGVFTHSSLNHNDIGLEYVKDTSGKFVAINGSTVQVTNEG
                     LAHSLGFNRASDLNLPEEWDTTSSRYAYKGAIVSTVTSGNTYTVTFGQGDMPQNVGLS
                     YWFALNTLPVARTVTPYSPKPHVAVELEPVPEPITVTPDVFTPKTFTPEKPVTFTPKP
                     LEEVVQPSLTLTKVNLPVKPIPKELPTPPQVPTVHYHAYRLTTTPEIMKEVVNSDQAN
                     LHEKTVAKDSTVIYPLTVDALSPNRAQTTSLIFEDYLPAGYLFDKETTQKENGNYVLS
                     FDATKNFVTLTAKENLLQEVNKDLTKVYQLNAPKLYGSVQNDGATYSNSYKLLLNKGT
                     TNAYTVTSNVVTVRTPGDGETTTLITPDKNNENADSVLINDTVVALGTTNHYRLTWDL
                     DQYKGDRSAKETIARGFFFVDDYPEEVLDVVENGTAVTTLDGQKVSGITVKNYASLNE
                     APKDLQDKLARAKITPTGAFQVFLPDDNQAFYDQYVQTGTSLALLTKMTVKDSLYGQT
                     KTYTNKSYQVDFGNGYETKEVTNTLVSPEPKKQNLNKDKVDINGKPMLVGSQNYYTLS
                     WDLDQYRGIKADNSQIAQGFYFVDDCPEEALLPDEAAIQFITSDGKTVSGITVKAYSQ
                     LSEAPKMLQAALSKQKIQPKGAFQVFMPEDPQAFFESYVTKGENITIVTPMTVLETML
                     NSGKSYENVAYQVDFGQAYETNTVTNFVPKVTPHKSNTNQEGISIDGKTVLPNTVNYY
                     KIVLDYSQYKDMVVTDDVLAKGFYMVDDYPEEALTLNPDGIQVLDKDGNRVSGISVST
                     YASLSEASNVVQDAMAKRQFTTKGAIQVLSSDDPKTFYETYVKTGQTLVVTLPMTVKN
                     ELTKTGGQYENTAYQIDFGLAYVTETVVNNVPKLDPQKDVVIDLSHKDDSLDGKEVAM
                     NQTFNYRLVGAMIPSNRATDLFEYGFEDNYDEKHDEYNGVYRSYLMTDVILKDGSVLK
                     EGTEVTKYSLQQVDTENGLVSISFDKSFLETISDDSAFQADVYLQMKRIATGQVENTY
                     LHTVNGYVISSNTVVTHTPQPEEPSPNQPTPPQPPIETIEPPVPASILPNTGEQESLL
                     GLIGAGILLGTAYGLKKKEEK"
     gene            5444..5635
                     /locus_tag="ACHU66_002205"
     CDS             5444..5635
                     /locus_tag="ACHU66_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFR3390430.1"
                     /translation="MNPKTIYEKDSDQDGLTDAQELALGTNPQSVDTDGDGQADLEEL
                     QSGHSPLVPQKELCDGLEL"
     gene            5619..6170
                     /locus_tag="ACHU66_002206"
     CDS             5619..6170
                     /locus_tag="ACHU66_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR3390431.1"
                     /translation="MAWNFDTMKEALSEMEKVNYQEFIKAFLSLELSISDRTILNQVY
                     QDYMDEDDLSLISDELRVKVEGYQDEVKADMTDILEKLYRTGEGSSFIMDLMSSNSLS
                     DTLEQYEVLDSDDYSPLSLETLQAMIQQDLAISSQDYFGDLVHLALQKDLLDQKSHFL
                     QYYVATVMEGIPQERDQRALVLD"
     gene            6220..12183
                     /locus_tag="ACHU66_002207"
     CDS             6220..12183
                     /locus_tag="ACHU66_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFR3390432.1"
                     /translation="MNQEVLLQMMRATIPRDRALLEAFLYYQAEHFDEEWDSLVYQFM
                     TNRQEINKSVQVLHFETDVSAFVQASPYDTAHDLLTYTQVFGQTGLQKLDKLSPSEKD
                     LVIEVALFNLATRFQLLDSNGHYQTISPDSLLQKSRGANLVNVYRVANNLAERISRDI
                     EQFLLTYEPELETRVEETVQEKEETVDEHKTSVHQAISFREEGSLVIASLDVDLSQLD
                     IRTGKTSHLPAYEELSLRRKFEILTYFDQIRNERSKLPNLRRGDFDTEMEKTPIFDGE
                     ELLTYLEADGSLYELKRTLTTVEEKELEKIGQAIRTENQEKLTQVGIDLSQFDSDQIG
                     ILLDAAGRFHLKNADLALLGGYPNASVTQLALATELLQMGLSHEKIEFFFGSQLSIEE
                     LRQVAYAFLHQELSIEDAEQFEKDKGNQPDLTLRDWKNKLEKAEGKEVVDEAFEVNPL
                     VQMVLDTFPLGSLVSYKGQDFEVVSVSDARLNGLIRIELVNDFSDIIEQNPVLYLRTW
                     EEVSQALHQPKAEPQTELDDEDQELNLFSFLEEETSQSVNEAVQNIGVLDPEQSGEAS
                     HDSEKPTIYVPEEVLVESIPDIPATAFYFPEDMTDFYPKTARDKVETNIAAIRLVKTL
                     ETERRQASTSEQELLAKYVGWGGLANDFFDDYSPKFSKEREELKSLVTDKEYSDMKQS
                     SLTAYYTDPALIRQMWDKLERDGFTGGKILDPSMGTGNFFAAMPKHLREKSELYGVEL
                     DTITGAIAKHLHPNSHIEIKGFETVAFNDNSFDLVISNVPFANIRIADNRYDKPYMIH
                     DYFVKKSLDLVHDGGQVAIISSTGTMDKRTENILQDTRETTEFLGGVRLPDSAFKAIA
                     GTSVTTDMLFFQKHLDKGYLADDLAFSGSIRYEKDSRIWLNPYFDGEYNSQVLGTYEV
                     RNFNGGTLSVKGTSDDLIASVQTALNHVKAPREIDRNEVIINPNVLTKQVIDTSIPAE
                     MRENLGQYSFGYQGSTVYYRDNKGIRVGTKMEEISYYVDEEGNFKAWDTKHSQKQIDR
                     FNNLDVTDSTALDVYVTDDAAKRGQFKGYYKKTVFYEAPLSDKEVARIKGMVDIRNAY
                     QEVIAIQRHYDYDKKTFNHLLGKLNRIYDSFVKRYGYLNSTVNRNLFDSDDKYSLLAS
                     LEDESLDPSGKSVIYTKSLAFEKALVRPEEEVKKVHTALDALNSSLADGRGVDFAYMM
                     SIYQVESKMTLIEELGDLIMPDPEKYLNGELTYVSRQDFLSGDVVTKSEVVDLFVKQD
                     NQDFNWSHYAGLLEAIKPARITLADIDYRIGSRWIPLSVYGKFAQETFMGKSYELSDQ
                     EVATVLEVSPIDGVITYQSKFAYTYSNATDRSLGVPASRYDSGRKIFENLLNSNQPTI
                     TKQVVEGDKKKNVTDVEKTTVLRAKETHLQQLFQDFVSKYPEVQQMIEDTYNRRYNRT
                     VSKVYDGSHLTIDGLAQNISLRPHQKNAIQRIVEEKRALLAHEVGSGKTLTMLGAGFK
                     LKELGMVHKPLYVVPSSLTAQFGQEIMKFFPTKKVYVTTKKDFAKAKRKQFVSRIITG
                     DYDAIVIGDSQFEKIPMSREKQVTYINDKLEQLREIKLGSDSDYTVKEAERSIKGLEH
                     QLEELQKLERDTFIEFENLGIDFLFVDEAHHFKNIRPITGLGNVAGITNTTSKKNVDM
                     EMKVRQVQAEHGDRNVVFATGTPVSNSISELFTMMNYIQPDVLERYQVSNFDSWVGAF
                     GNIENSMELAPTGDKYQPKKRFKKFVNLPELMRIYKETADIQTSDMLDLPVPEAKIIA
                     VESELTQAQKYYLEELVERSDAIKSGSVDPSRDNMLKITGEARKLAIDMRLIDPVYSL
                     SDNQKILQVVDNVERIYREGAGDKATQMIFSDIGTPKSKEEGFDVYNELKVLLVDRGI
                     PKEEIAFVHDANTDEKKNSLSRKVNSGEVRILMASTEKGGTGLNVQSRMKAVHHLDVP
                     WRPSDVGRILRTFKIKKNVEVTDNGKDNF"
     gene            12164..12538
                     /locus_tag="ACHU66_002208"
     CDS             12164..12538
                     /locus_tag="ACHU66_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HFR3390433.1"
                     /translation="MAKTIFEEMGGKYERQGDYLIPCLTVPAEEEQPIGIWGQRHLDY
                     LKHHCKVTYTNLLTSGRLNAYLADIDRQAQERFERLIEGMKQAQGITEQLKAENALEW
                     TGCLNNIRACAREIVEKEIIFA"
     gene            12905..14824
                     /gene="tet(O)"
                     /locus_tag="ACHU66_002209"
     CDS             12905..14824
                     /gene="tet(O)"
                     /locus_tag="ACHU66_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(O)"
                     /protein_id="HFR3390434.1"
                     /translation="MKIINLGILAHVDAGKTTLTESLLYTSGAIAEPGSVDKGTTRTD
                     TMNLERQRGITIQTAVTSFQWEDVKVNIIDTPGHMDFLAEVYRSLSVLDGAVLLVSAK
                     DGIQAQTRILFHALQTMKIPTIFFINKIDQEGIDLPMVYQEMKAKLSSEIIVKQKVGQ
                     HPHINVTDNDDMEQWDAVIMGNDELLEKYMSGKPFKMSELEQEENRRFQNGTLFPVYH
                     GSAKNNLGIRQLIEVIASKFYSSTPEGQSELCGQVFKIEYSEKRRRFVYVRIYSGTLH
                     LRDVIKISEKEKIKITEMCVPTNGELYSSDTACSGDIVILPNDVLQLNSILGNEMLLP
                     QRKFIENPLPMLQTTIAVKKSEQREILLGALTEISDGDPLLKYYVDTTTHEIILSFLG
                     NVQMEVICAILEEKYHVEAEIKEPTVIYMERPLRKAEYTIHIEVPPNPFWASVGLSIE
                     PLPIGSGVQYESRVSLGYLNQSFQNAVMEGVLYGCEQGLYGWKVTDCKICFEYGLYYS
                     PVSTPADFRLLSPIVLEQALKKAGTELLEPYLHFEIYAPQEYLSRAYHDAPRYCADIV
                     STQVKNDEVILKGEIPARCIQEYRNDLTYFTNGQGVCLTELKGYQPAIGKFICQPRRP
                     NSRIDKVRHMFHKLA"
     gene            14876..15049
                     /locus_tag="ACHU66_002210"
     CDS             14876..15049
                     /locus_tag="ACHU66_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002779755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFR3390435.1"
                     /translation="MMKCEWILCPVCGSKTRNKIRKDTVLENYPLYCPKCRQESLIKV
                     DNLKITVIKEPDA"
     gene            15539..>15964
                     /locus_tag="ACHU66_002211"
     CDS             15539..>15964
                     /locus_tag="ACHU66_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFR3390436.1"
                     /translation="MKYAPRKVYIKESGGYVELSYTDFCRRRQADKGYMDKLFIPVQG
                     CLLEVVREQYADFYRDRERWRYLQKLDARNSLLSLDGFTDSEGNPLDFIADEAADIAE
                     TVVNAVMVDRLKAALPLLSDSEQELIQAIFFDGLSEREVG"
BASE COUNT         5086 a         3022 c         3528 g         4328 t
ORIGIN      
        1 tattgtggaa tttcatctgt aaactgttgt agatacagtg ccgaaatata agagaaaata
       61 gccttaggaa aacgatattg aaagaagtag tattcatcat agtctccgtt ttgggttagg
      121 aaaatacctt tttcaactcg gaagataatg ccttctttct ctagtcttgt cagatatatt
      181 gttggaatac ctagtgcttt acaatctttg tttgtgacaa ttccattgtt cttttctata
      241 aaatcgagta gaatctcttt ttttgacata cggcctccca cttgttgctt ttacactaca
      301 attttacaat atttgtggta taaaagcaac attttctttt ccctcatttt attgtgataa
      361 caaaatcaag ccttcttttt tatgatggta ttgaacttgg gctacgagct gtacaaaaaa
      421 gagagtttct cctagaacgg aaagttcttc gtcaaaactc ctatttttgt cgtgctcgtt
      481 taacgccctc gtatctaata aagagtgaca gtttgttgct acataaaaaa gaaaagaagg
      541 actatttatg accaaaacat gtaatcatca ctttcttgtc aatcaggaaa aaggcgagaa
      601 acacgtcttt cgtaagagta aaaaataccg tactctatgt tcggttgccc ttggaacgat
      661 ggtgacggct gttgttgctt ggggaggaac ggttgcacat gctgatgaag tcacatcatc
      721 agttgacacc aacatccaac gaacggagaa tccagccaca aatttaccag aagcacagcc
      781 aaaacctgta tctgaacaaa ctgaaatttt agcgttaact ggacaatcta atggagcgat
      841 tgctgttacc gtaccacatg atacggtaac acaagcagtt gaagaggcaa aggctgaagg
      901 tgtttctacg gttgaagata gccaaatgga tttaggaaat acaacttctg cggcagaaac
      961 taaccaacaa atttcaaaag cagaagcaga tgcccaaaat caagttgagg ctatcaacga
     1021 agttactgaa acctacaaag ctgacaaagc agcttatgtg gatgaaaaag ctcgcatcga
     1081 acaggaaaat aaggagctgt cacaggccta cgaaggggcc aaccaaactg gtaaagagac
     1141 aaatacttgg gttgatacca aagtcaaaga cctaaaaact cggtatgcag atgctgatgt
     1201 gacagtaaaa gaacaagtag tctcatcagg aaacggaaca tctgtactgg actatacaaa
     1261 ctatggcaag gctgttgaaa ctattcaatc aactaacgaa caagctgtag cggattatct
     1321 aacaaagaaa accaaggcag atgatattgt tgcgaaaaat caggccattc aaaaagaaaa
     1381 tgaagctgga cttgctaagg caaaggcaga taatgaagcc attgaaaggc gtaatcaggc
     1441 aggtcaagca gctgttgatg ctgaaaaccg tgcaggtcaa gccgcagtag atcaagctaa
     1501 tcaggagaaa caacaattag tttcagatcg tgcagccgag attgaagcta tcacaaaacg
     1561 taatcaagaa aaagaagccg cagccagaaa agaaaatgaa gtgattgatg cctacaatac
     1621 caaagaaatg gaacgctatc aacgtgactt ggccgagatt tcgaaaggtg aagaaggtta
     1681 tatttctgaa gcccttgctc aagccctcaa tctcaataac ggtgaacccc aagcacaaca
     1741 tggagcaatt acccgaaatc ctaatcaaat catttcaact ggcgatgcca tgctgggtgg
     1801 ctactcaaga atattggatt caacaggatt ctttgtctat gatagcttca aaacaggtga
     1861 gacccttagt tttaactatc aaaatcttca aaatgcacga tttgatggta aaaagattag
     1921 tcgagtgact tacgatatta ccaaccttgt atcaccagct ggaaccgatg ccgtgaaatt
     1981 ggttgtgcca aatgatccaa ccgaaggttt tattgcctat cgaaatgatg gaaatggtga
     2041 ttggcgaaca gacaagatgg aatttcgtgt agttgccaag tatttcttgg aagacggaac
     2101 gcaggttacc ttctccaaag aaaaaccagg agtcttcacg cattcttccc tcaatcataa
     2161 tgacattggt ctagaatatg tcaaagatac ttctgggaaa tttgtggcga ttaatggttc
     2221 aaccgttcaa gtgactaatg aaggtctagc ccattctttg ggtttcaacc gtgcaagtga
     2281 tttgaatctg cctgaggaat gggataccac atcaagtcgt tatgcttata aaggagcaat
     2341 tgtctcaaca gttacatcag gcaataccta cacagtaacc tttggccaag gcgatatgcc
     2401 acagaatgtt ggattgtctt actggtttgc cttaaatacc ctaccagttg cacgtacagt
     2461 gacaccttat agtcccaaac ctcatgtagc ggtggaactt gaacccgttc cagaacctat
     2521 tacggtaaca ccagatgtct ttactccaaa aacctttaca cctgaaaagc ctgtgacctt
     2581 cacgccaaag cctttggaag aagtagtgca gcctagtcta actttgacca aggtaaactt
     2641 acctgttaaa cctattccaa aagaacttcc aacgccacca caagttccga ctgtccatta
     2701 tcatgcgtac cgtttgacga caactccaga gattatgaaa gaagtagtca atagtgacca
     2761 agctaatctt catgagaaaa ctgtcgcaaa agattcaacg gtgatttatc ccttaacagt
     2821 tgatgcctta tcgcccaatc gtgcccaaac gactagtctc atttttgagg actacttgcc
     2881 tgctggttac ctatttgata aggaaacaac acaaaaagag aatggaaact atgtccttag
     2941 ctttgatgcg actaagaatt ttgtgacctt aaccgcaaag gaaaacttgt tgcaggaggt
     3001 aaataaagat ttaaccaagg tttatcaact gaacgcccca aaactctatg gttctgttca
     3061 aaatgatggg gcaacctatt ccaatagtta caaactcctt ttgaacaagg gcacaacaaa
     3121 tgcttacaca gtcacttcaa atgttgtaac ggttcgtaca ccaggtgatg gggagacaac
     3181 cacactcatt acaccagata aaaacaatga aaatgcggat agtgtcctca ttaatgacac
     3241 ggtcgtagcc cttggcacaa ccaaccacta tcgtttgact tgggatttgg accagtataa
     3301 gggagatcgt tctgctaaag agacaattgc acgaggcttc ttctttgtgg acgattaccc
     3361 agaggaagtg cttgatgtgg tggaaaatgg gacggctgtt acaacccttg acggtcagaa
     3421 ggtatcagga attacggtta aaaactatgc ttccctaaat gaagccccta aagaccttca
     3481 agataaattg gctcgtgcta agattacacc gacaggtgcc tttcaagtct ttttgccgga
     3541 tgacaaccaa gccttttatg accagtatgt tcaaacagga acttctttag ctcttttgac
     3601 caaaatgacg gtgaaagata gtctctatgg tcaaactaag acctatacaa acaagtctta
     3661 tcaagttgat tttggaaatg gctatgaaac caaggaagtg accaacaccc ttgtttctcc
     3721 agaacccaag aaacagaacc tcaataaaga caaagtggat atcaatggga agcccatgct
     3781 agtgggaagt caaaattact atactctctc atgggacttg gaccaatacc gagggattaa
     3841 agcagacaac tctcagattg cacaaggttt ttactttgtg gatgattgtc cagaagaagc
     3901 tttattgccg gatgaagcag ctattcagtt tatcacatct gatggcaaaa cagtttcagg
     3961 aatcacggtg aaggcttatt ctcaattatc agaagctcct aaaatgctac aagcagccct
     4021 ttcgaaacaa aaaattcagc ctaaaggagc ttttcaagtt ttcatgcctg aggacccaca
     4081 agcctttttt gaatcttatg tgaccaaggg ggagaatatt accattgtca ctccgatgac
     4141 ggttttggaa accatgctta attcagggaa gtcttatgaa aacgtggctt atcaggtgga
     4201 ctttgggcaa gcctatgaaa ccaacacggt gaccaatttt gtccctaaag taactccaca
     4261 caagtctaat accaatcaag aaggcatttc aattgatgga aagactgttc ttccgaatac
     4321 ggtcaattat tacaaaattg tcttggatta cagtcaatac aaggacatgg tcgtgacgga
     4381 tgatgttctt gccaagggat tttacatggt agacgattac cctgaagaag cccttaccct
     4441 aaatcctgat ggcattcaag ttttggataa ggatggcaat cgtgtatctg gtatctctgt
     4501 cagcacctac gctagtttgt cagaagcttc gaatgtcgtc caagatgcca tggctaaacg
     4561 tcagtttaca actaaaggag ccattcaggt ccttagtagc gatgatccca aaacctttta
     4621 tgagacctat gtgaagactg gtcaaacctt agttgtcacg cttccgatga cggtaaaaaa
     4681 tgagttgacc aaaacaggtg gtcagtatga aaatacagcc tatcagattg attttggctt
     4741 ggcctatgtc acggaaacag tggttaataa tgttcccaaa ctagacccac aaaaagatgt
     4801 ggtgattgac ttgtctcata aagatgacag ccttgatggt aaagaagtgg ccatgaatca
     4861 aacctttaac tatcgtttgg ttggagcgat gatcccaagc aatcgtgcga cagatttatt
     4921 tgaatatggt tttgaagata actatgatga aaagcatgat gagtataatg gtgtttatcg
     4981 tagctacctg atgacggatg tcatcctcaa agacggttct gtcttaaaag aggggacaga
     5041 agtcacgaaa tatagcttgc aacaggtgga tacagaaaat ggcctagtgt caatttcatt
     5101 tgataaatcc ttcttagaga ctatctctga tgattcagct tttcaggcag atgtttacct
     5161 gcagatgaaa cggattgcga ctggtcaggt ggagaatacc taccttcata cagtgaatgg
     5221 ctatgtcatc agctcaaata cagttgtaac acatacacct caacctgaag aaccaagtcc
     5281 aaatcaaccc acaccacctc aaccaccaat tgagactatt gaaccgcctg ttccagcaag
     5341 cattttgcca aatacagggg aacaggaatc ccttttgggc ttgattggag ctggtattct
     5401 acttggtacg gcttatggac tgaagaaaaa ggaggagaag tagatgaatc caaaaactat
     5461 ttatgaaaag gattcagacc aagatggttt gacagatgct caggaactag ctttggggac
     5521 caatccgcag tctgttgaca cagatggtga tggtcaagct gatttagagg agctacaatc
     5581 tggacattca ccactagtcc cacaaaagga gttgtgtgat ggcttggaac tttgacacca
     5641 tgaaagaggc tttgtctgaa atggaaaagg tcaattacca agagtttatc aaagcctttc
     5701 tctctttgga actaagcatt tctgatagaa caatcctcaa tcaagtttat caagattata
     5761 tggatgaaga tgacctgtcc ttgattagtg atgagctacg agttaaagtg gaaggttatc
     5821 aggatgaagt aaaagctgat atgactgata ttctggaaaa gctgtaccga acaggtgaag
     5881 gctctagttt tattatggat ctaatgtcct caaatagtct ttcagacacc ttggaacaat
     5941 atgaagtttt ggatagtgac gattattcac cacttagcct tgaaacctta caggccatga
     6001 ttcaacagga cttggccatt tccagtcaag attattttgg agatctggtt caccttgcct
     6061 tgcaaaaaga tttactagac cagaaaagtc atttcttaca atactatgtg gcaactgtaa
     6121 tggaaggtat tccacaagaa agagaccaac gtgccttggt cctagattaa cgcaaagggc
     6181 tgagaaacac tctcaacctt ttttaaatgg gagggaaaaa tgaatcaaga agtcttacta
     6241 caaatgatga gagccaccat tcctcgtgat agagccttgc ttgaggcgtt tttatattac
     6301 caagcagagc attttgatga ggagtgggat agtctcgttt atcagtttat gactaatagg
     6361 caagaaataa ataagtctgt tcaggtactt cattttgaga cagatgtttc agcttttgtt
     6421 caggctagtc cttatgatac tgctcatgat ctattgacct atacacaagt attcggccaa
     6481 actggtctcc aaaaactcga caaactatcg ccgtctgaaa aagacttggt gattgaagtg
     6541 gccttgttta atctggccac acgttttcaa ttattagatt ccaatggtca ttaccaaact
     6601 atctcaccag attcgctttt acaaaagagt aggggagcca atttggtcaa tgtttaccgt
     6661 gtggctaata atttagcgga acgcattagc cgagatattg aacagtttct cttgacttac
     6721 gagcctgagc ttgaaacgag agttgaggaa acagttcaag aaaaagaaga aactgttgat
     6781 gagcacaaaa caagtgttca tcaagcaata tcttttcgag aagagggctc cctggttatt
     6841 gctagtttgg atgtagattt gtctcaactg gatatccgaa ctggaaaaac cagtcatcta
     6901 ccagcttatg aagagttatc tttacgacgt aaatttgaga ttctaaccta ttttgaccag
     6961 attcgaaatg aacgttcaaa acttccgaat cttagacgag gtgattttga tactgagatg
     7021 gaaaagacac caatctttga tggcgaggag ttacttacct atctagaggc tgatggcagc
     7081 ctctatgagc tgaaacggac actgactaca gtcgaagaaa aggaattaga aaaaattgga
     7141 caagccatta ggacagaaaa tcaagaaaaa ttgactcagg tagggattga tttatctcag
     7201 tttgactctg accaaatcgg tattttattg gatgccgcag gtcgttttca tctgaaaaat
     7261 gcggatcttg cattattagg tggctatcca aatgcctctg taactcaact agcccttgcg
     7321 acagaactac tccaaatggg attgagtcat gaaaaaattg aatttttctt tggtagtcag
     7381 ctttccattg aagagctgcg acaagttgcc tacgcctttt tacaccaaga acttagcata
     7441 gaagacgcgg agcaatttga aaaagataaa ggcaatcagc cagatttaac cctcagagat
     7501 tggaagaaca agctagagaa agctgaggga aaagaagtag ttgatgaagc gtttgaggta
     7561 aatccacttg ttcagatggt tttggacact tttcctcttg ggtcattagt ttcctacaag
     7621 ggacaggact ttgaggtcgt ttcggtcagc gatgctcgat tgaacggttt gattcggatt
     7681 gagttagtca atgacttttc ggatatcatt gaacaaaatc cagttcttta tttgaggacc
     7741 tgggaagaag ttagtcaggc acttcatcag ccaaaggcag aaccacaaac agagttagac
     7801 gatgaagacc aagaattaaa ccttttttca tttctggaag aggaaacaag tcaatctgtt
     7861 aatgaagctg ttcagaacat tggagttttg gatccagaac aatcgggaga agctagtcat
     7921 gacagtgaaa agccaaccat ttatgtacct gaagaagtgc ttgtagaatc gattccagat
     7981 attccagcaa cggctttcta ctttccagaa gatatgacgg acttttatcc taagactgct
     8041 agagataagg ttgagacaaa cattgcggct attcggttgg ttaaaacact tgaaacggaa
     8101 cgccgtcaag caagtacaag tgaacaagaa ctccttgcca agtatgtggg ctggggtgga
     8161 cttgccaatg atttctttga tgactatagt ccaaaatttt ctaaggaacg ggaagaactg
     8221 aaaagcctag tcacagataa agagtattcg gatatgaaac agtcctctct gacagcctat
     8281 tatacagacc ctgcccttat ccgtcagatg tgggataagt tggaaagaga tggctttaca
     8341 ggtggcaaaa tcctagatcc ttccatggga acagggaatt tttttgcggc tatgccaaaa
     8401 cacttaagag aaaagagtga gctatatggc gtagagttag atactattac aggagctatt
     8461 gccaaacacc ttcatcccaa tagtcacatt gaaattaagg gctttgagac ggtggctttt
     8521 aacgacaata gttttgattt ggtgatttca aatgtgccct ttgccaatat acgaattgcg
     8581 gataataggt acgataagcc ttatatgatt catgattact ttgtcaaaaa atctcttgat
     8641 ttagtccatg atggtggaca agtagcgatt atctcttcca caggaaccat ggataagcga
     8701 acagaaaaca tcttacaaga tactcgtgag acaactgaat ttcttggtgg ggttcgactg
     8761 cctgattctg cctttaaggc cattgcagga acgagtgtca caacggatat gttattcttc
     8821 cagaaacact tagacaaggg atatttggca gacgacttag ccttttcagg ttctattcgc
     8881 tatgagaagg atagtcgcat ttggctcaat ccttactttg atggagaata caatagccaa
     8941 gtgctaggaa cctacgaggt cagaaatttt aacggaggaa cactttctgt taaggggact
     9001 agtgatgact tgattgcaag tgtccaaact gctctaaatc acgttaaggc cccaagagag
     9061 attgatagaa atgaggtcat cattaatcca aatgtgttga ccaaacaagt cattgatacc
     9121 tccattccag ctgaaatgag ggagaatctg ggtcagtaca gttttggtta ccagggttct
     9181 acagtttact atcgagacaa caaaggcatt cgagtcggaa ccaagatgga agaaatcagt
     9241 tactatgtcg atgaagaggg caacttcaaa gcttgggaca ccaaacattc ccaaaaacag
     9301 attgatagat ttaataactt agatgtgact gatagcactg ctctggatgt ctatgtgacc
     9361 gatgatgcag ctaaacgcgg acaatttaag gggtattata aaaagacagt tttctatgaa
     9421 gctcctttgt ctgataaaga agtggcacga atcaaaggaa tggtcgatat tcgcaatgct
     9481 tatcaagagg tcattgccat tcaacgccat tatgactatg ataagaagac ctttaaccac
     9541 ttgttaggca aactcaatcg tatctatgat agcttcgtca aacgctatgg atatttgaat
     9601 agtactgtga accgcaatct tttcgatagt gatgacaagt attcgcttct tgctagtttg
     9661 gaagatgaaa gtctggatcc aagtggaaag tctgttatct atactaaatc ccttgccttt
     9721 gagaaggctc tagtgcgtcc tgaagaagag gttaaaaagg tacatactgc ccttgatgcc
     9781 ttaaattcga gcttggctga cggacgaggt gttgattttg cttatatgat gtctatctat
     9841 caggttgaat cgaagatgac tttgattgag gagttaggag acctcattat gcctgatcct
     9901 gagaagtatt tgaatggaga attgacctat gtttctcgtc aagactttct gtcaggtgat
     9961 gttgttacca agtcagaagt ggtggaccta ttcgtgaaac aagacaatca ggactttaac
    10021 tggtcacatt atgcaggact tcttgaagct atcaaaccag cccgtattac tttggcagac
    10081 attgattatc gaataggttc acgctggatt cctttgtctg tttacggaaa atttgcccaa
    10141 gaaaccttta tggggaaatc ctatgaactg tcagaccaag aagtagcaac agtccttgaa
    10201 gtcagtccca ttgacggggt tatcacttac caatctaagt ttgcctacac ctattccaac
    10261 gcaacggata ggagtttagg tgtccctgct tcacgctatg atagtggtcg aaaaatcttt
    10321 gaaaacctcc tgaattccaa tcaaccaacc atcacaaaac aagttgtcga aggagacaag
    10381 aaaaagaatg tgacagatgt ggagaaaaca acggtcctgc gtgctaagga aacgcacctc
    10441 caacaactct ttcaagattt tgtatcaaag tatccagaag ttcaacaaat gattgaagac
    10501 acctataata ggcgttacaa tcgtacagta tcaaaggtct atgatggtag ccatttaacc
    10561 attgatggac ttgcccagaa tatttcctta cgtcctcacc aaaaaaatgc cattcaacgg
    10621 attgtggagg agaaacgtgc cctactagcc catgaggtag ggtctggaaa aaccttgacc
    10681 atgcttggag ctggcttcaa gttgaaagaa ttaggaatgg tacataaacc actttatgtg
    10741 gtgccgtcta gtctgactgc tcagtttggt caagaaatca tgaaattctt cccaaccaag
    10801 aaagtctatg tgaccaccaa gaaagatttt gccaaagcca aacgtaagca gtttgtgtcc
    10861 cgtattatta caggggacta tgatgccatt gtcattgggg attcacaatt tgagaagata
    10921 ccaatgagtc gtgaaaaaca ggtcacctat atcaatgaca aactcgagca actccgagaa
    10981 atcaagctag gaagtgacag tgattacacg gtgaaagaag cggaacgttc gattaaggga
    11041 ttggaacacc agttggaaga actccaaaaa ctagagcgag atacctttat cgaatttgaa
    11101 aaccttggaa ttgattttct ttttgtggat gaggctcatc acttcaagaa tattcgtcca
    11161 atcactggac ttgggaatgt cgcgggaatc accaatacta cttcaaaaaa gaacgtagat
    11221 atggagatga aggtgagaca ggttcaggca gagcatggcg atagaaatgt tgtttttgcg
    11281 acaggaacac cagtttctaa ctctattagt gaacttttca ccatgatgaa ttacattcaa
    11341 cctgatgttt tagagcgata ccaggtatcc aattttgatt catgggttgg ggcttttggg
    11401 aatattgaaa actctatgga actagccccg acaggagata agtaccaacc caagaaacgg
    11461 ttcaagaaat ttgtcaacct tcctgaactc atgcgaatct acaaggaaac tgccgatatt
    11521 cagacctcag acatgcttga tttaccagta cctgaagcta agattattgc ggtggaaagc
    11581 gagttaacgc aagctcagaa atactattta gaagagctgg tagagcgttc ggacgctatc
    11641 aagtcaggta gtgttgatcc aagtagagat aacatgctta agataacagg tgaagccaga
    11701 aaactagcta ttgatatgcg gttgattgac cctgtttact ccttatcgga taatcagaaa
    11761 atccttcaag tagtggataa tgtcgaacga atctaccgtg aaggagctgg agacaaagcc
    11821 actcagatga ttttctctga tattggaacc cctaaaagta aggaagaagg gtttgatgtc
    11881 tacaatgaac tgaaagtttt gctggttgat cgagggattc caaaagaaga aattgccttt
    11941 gttcatgatg ccaatactga tgagaagaaa aactctctct cacgaaaggt caatagcgga
    12001 gaagtacgga ttctcatggc ttctacggaa aaaggtggaa cgggtctaaa cgtgcaatca
    12061 cgcatgaaag ctgtccacca cttagatgtt ccctggcgtc cttctgatgt aggacggatt
    12121 ttgcggacat tcaaaataaa aaagaatgtg gaggtaacag acaatggcaa agacaatttt
    12181 tgaggaaatg ggcggcaaat acgaaaggca aggcgattat ttaataccgt gcttaactgt
    12241 acccgccgaa gaagaacagc cgataggcat ctggggacag cggcatttgg attatctgaa
    12301 gcatcactgc aaagttacat acaccaatct tcttacaagc ggcagactta acgcttacct
    12361 tgccgacatt gacagacagg cacaggaacg ctttgaaagg ctcatagagg gtatgaaaca
    12421 ggcacagggc ataacggaac agctaaaggc agaaaacgcc ttagaatgga caggatgcct
    12481 caataacata agggcttgtg cgagggagat tgtggaaaag gaaattattt ttgcataaac
    12541 agatgattag tggcaggggg aaatcctgcc gctttttctg ctttagtttg tcagcttgac
    12601 aaataaaggg ttaaggaata taattagatt cagtattata caaggagtta ataaatatgc
    12661 ggcaaggtat tcttaaataa actgtcaatt tgatagtggg aacaaaaagt agcagtcccg
    12721 tttcactttt aatatggggc ttagtttttt gtacccagtt taagaatact tttatcatgt
    12781 aattttatat gcccgaaaac atataagtgt tttggggcta ttggagttat ttacccagtg
    12841 ataggagtat ttatcactgg gtatttttat gccctttttt gggtgttgat aggaggaaaa
    12901 tcacatgaaa ataattaact taggcattct ggctcacgtt gacgcaggaa agacaacatt
    12961 aacggagagt ttattgtata ccagtggtgc aattgcagaa ccagggagcg tagataaagg
    13021 cacaacaagg acagatacaa tgaatttgga gcgtcaaagg ggaatcacta tccagacagc
    13081 agtgacatct tttcagtggg aggatgtaaa agtcaacatt atagatacgc caggccatat
    13141 ggattttttg gcggaagtat accgttcttt atccgtatta gacggagcag tattattagt
    13201 ttctgcaaag gatggcatac aggcacagac ccgtatactg tttcatgcac tacagacaat
    13261 gaagattccg acaatttttt tcatcaataa aattgaccaa gaggggattg atttgccaat
    13321 ggtatatcaa gaaatgaaag caaagctttc ttcggaaatt atagtgaagc aaaaggttgg
    13381 gcagcatccc catataaatg taacggacaa tgacgatatg gaacagtggg atgcggtaat
    13441 tatgggaaac gatgaactat tagagaaata tatgtcaggg aaaccgttta aaatgtcaga
    13501 actggaacag gaagaaaaca ggagattcca aaacggaacg ttatttcccg tttatcacgg
    13561 aagtgctaaa aacaatctgg ggattcggca gcttatagaa gtgattgcca gtaagtttta
    13621 ttcatcaacg cctgaaggtc aatctgaact atgcgggcag gtttttaaga ttgaatattc
    13681 agagaaaagg cggcgttttg tttatgtgcg tatatatagc ggaacattgc atttgaggga
    13741 tgttattaaa atatctgaaa aagagaaaat aaaaatcaca gagatgtgtg ttccgacaaa
    13801 cggtgaatta tattcatccg atacagcctg ctctggtgat attgtaattt taccaaatga
    13861 tgttttgcag ctaaacagta ttttggggaa cgaaatgctg ttgccgcaga gaaaatttat
    13921 tgaaaatcct ctccctatgc tccaaacaac gattgcagta aagaaatctg aacagcggga
    13981 aatattgctt ggggcactta cagaaatttc agatggcgac cctcttttaa aatattatgt
    14041 ggatactaca acgcatgaga ttatactttc ttttttgggg aatgtgcaga tggaagtcat
    14101 ttgtgccatc cttgaggaaa aataccatgt ggaggcagaa ataaaagagc ctactgttat
    14161 atatatggaa agaccgctta gaaaagcaga atataccatc cacatagaag tcccgccaaa
    14221 tcctttctgg gcttctgtcg ggttgtccat agagccgctc cctattggaa gcggagtgca
    14281 gtatgaaagc agagtttcac ttggatattt aaaccaatcg ttccaaaatg cggttatgga
    14341 gggggttctt tatggctgcg agcaggggct gtatggatgg aaagtgacag actgtaaaat
    14401 ctgttttgaa tatggattgt attatagtcc tgtaagtacc cccgcagact ttcggctgct
    14461 ttcccctatc gtattggagc aggctttaaa aaaagcaggg acagaactat tagagccata
    14521 tctccacttt gaaatttatg caccgcagga atatctctca cgggcgtatc atgatgcccc
    14581 aaggtattgt gcagatattg taagtactca ggtaaagaat gacgaggtca ttctgaaagg
    14641 agaaatccct gccagatgta ttcaagaata caggaacgat ttaacttatt tcacaaatgg
    14701 gcagggagtc tgcttgacag agttaaaagg ataccagcca gctattggta aatttatttg
    14761 ccaaccccgc cgcccgaata gccgtataga taaggttcgg catatgttcc acaagttagc
    14821 ttaacagctt gcaaaagtca tataaaatga gatttgaaag gattagagac taattatgat
    14881 gaaatgcgaa tggatattgt gtcctgtttg tgggagcaaa acccgtaata aaattaggaa
    14941 ggacactgtt ttggagaatt atccccttta ttgtccaaaa tgcagacaag aaagcttgat
    15001 taaagttgac aacttgaaga taactgtcat caaagagcca gacgcttaag acgcagagcc
    15061 gatgaaattg tggaacaatt cacgaatcat cggctctttt tgtttcgtat ttgaaagaac
    15121 aaactcacca aataaaaaaa acgatattcg ggtgggttat tttgttatac cctaaattac
    15181 cctctgaatt tgtttttaaa tttggaggga tttttttatg ttcttttttc gggcggttat
    15241 tcatctgctc atacgaagta aaatttatca atacatagca tatcagagaa tggcaggaaa
    15301 ccagttaaaa aaatttctgc cagtgcagcg gtacttctca ccttgaaagt ggaagtacaa
    15361 tctccataca acagaatttg tatttcccat aaccgtgaag gtcacagagc ctttgcggtt
    15421 tttcttttgt cgttttttgt tggaaagtgc cgcagggctg tttctggtgt gcgttgccgc
    15481 tccccgcctc tccatctgga tttttacatt tcaaaaattc agattggagg tattagcggt
    15541 gaaatacgca ccaagaaaag tatatatcaa agaaagcggc ggctatgtgg aactgtccta
    15601 tacggatttc tgccgccgca ggcaagccga caagggatat atggacaagc tgtttatccc
    15661 cgtccaaggt tgtctgcttg aagtcgtaag ggagcaatat gcggacttct accgtgacag
    15721 ggagcggtgg cgttacctgc aaaaattaga tgcgaggaac agcctgctat ctctggacgg
    15781 atttacggac agcgagggga atcctctgga ctttatcgct gatgaagcgg cggacattgc
    15841 ggaaaccgtt gtcaatgctg ttatggtgga caggctgaaa gccgccctgc ctttgttgtc
    15901 ggatagtgag caggagttga tacaggcaat cttttttgac ggactttccg agcgtgaagt
    15961 cggg
//
LOCUS       DAZQCJ010000243         5223 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.guided.116, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000243 DAZQCJ010000000
VERSION     DAZQCJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.guided.116"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            147..1874
                     /locus_tag="ACHU66_002212"
     CDS             147..1874
                     /locus_tag="ACHU66_002212"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3390437.1"
                     /translation="MFRLIFDYIKRHKWLYLLVAVTLIIYDATLLLPTQIIQRMVDIL
                     TKNELTQAILVQEMTLLLLVTVLNYATAFIWHLKLFQASVNFKFDMQQRAFKKLVTMR
                     TPFYEKFRSGDVMTRFSTDVDGLMEMVGYGLMIVVYAGGMLAFIIPTMFFIDWKISLV
                     ALLPMLFMTLCIFFIGRKQDKAIDANREAVAQLNNEVLEVIEGIRVTRAYSKKATQKA
                     QFQARTKQLAQGGDRITSLQSLYNPLATVCLGLSTIFVLLMGAQAVKAGQLTLGQVIA
                     LQLYVGSLLEPFWTLADFILVYQTGKTSFEKLQELIETGDDLEADGSKEIAELSSISF
                     KNYSFSYPQAERASLQDINWTLKAGQTVGIVGKTGSGKTTLVRQFLRQYPIGQGNFFI
                     NHQSVLDFKRSSIEEKIGYVPQEHILFSRSVGENIALGKVASSSEEIEQAIATAAFSQ
                     DLKRMTDGLDTMIGERGVSISGGQKQRISIARAFLREPDLLILDDSLSAVDARTERQI
                     IQNIQKERAGKTNVIVTHRLSAVNHADWVLVLDEGRIVEEGRPADLLAQRGWYYEQYQ
                     RQQSQEGGE"
     gene            1874..3613
                     /locus_tag="ACHU66_002213"
     CDS             1874..3613
                     /locus_tag="ACHU66_002213"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR3390438.1"
                     /translation="MTILGFLLKQIRRVKWLFIVAVGFYLLASTMVRLAPLLIQQAID
                     GPITDLSKGLPFDEAVFLNQSAQYMGMIILGAIGFYLSMRLLMHCANRIAENLRNQAY
                     DVMQRLPISYFDDKPAGKIATRIVNDTETLRTQFYGTLVNAFNNIVRLLFTYGVLFYM
                     NRSLGWLMLLLIPLYIGIQFAYKKMTDKPMKDFYDARSDVNTQVNETMNGASLIQLFG
                     QEERIMKEFEVTADKMRRADNKIIWAQSLATWNLSGFLQNLVIAAILTVVGYQFLAGQ
                     YGVTAGKLFVYVNYIEGVFIALGALVQQFPNMLRSFETGKRLMALLEEEVEDDCDRIL
                     EVEQGQVVFEHVNFSYEENRPVLKDITIRAEKGETVALVGHTGSGKSSIMNLLYRFYD
                     PQEGRVLIDGKNIRDYSRESLRSHMGIVLQDPYLFTGTIASNVSMNEEEADRTRIMQA
                     LEKVGAGPMLSRLEKGIDEPVVEKGAAFSSGERQLIAFARTLYSDPKILILDEATSHI
                     DTETEEIIQHAMEVVKEGRTTFIIAHRLSTIQNADQILVLDQGRIIEHGKHEELVARG
                     GVYAQMHEIQAKV"
     gene            3667..4362
                     /locus_tag="ACHU66_002214"
     CDS             3667..4362
                     /locus_tag="ACHU66_002214"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFR3390439.1"
                     /translation="MKALIFDVDDTLYDQIQPFERALERHIEVAREQIEPLYLSFRRY
                     ADEVFEATAIGKMSLKDSHIYRMKHALADFGYQVSDATALAIQIDYDYFQGQIELSPV
                     FPEIFSWCQAQGIAMGIITNGPYRHQLRKIRTMGLVNWFELEHVLISGQVGITKPNPA
                     IFQLMEERLGMSGEDICYLGDSFENDVVGAKAANWKAIWFNHRKRVEPTAPYQADKVV
                     TDWDELVEVIQSF"
     gene            4516..5085
                     /locus_tag="ACHU66_002215"
     CDS             4516..5085
                     /locus_tag="ACHU66_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR3390440.1"
                     /translation="MELPMTQAFFPIHTLETVSPELRENLATVKKNNGGYIPNLIGLL
                     ANSPTALETYQTVSGINRRSSLNPTEREVVQITAAVANGCGFCVAGHTAISIKQVKMP
                     DVILQALRQGTPIETDAKLDALARFTLAVIHEKGKVEQPLLEEFFQEGYTAENALDVV
                     LGVSLATLCNYANNLINTPINPELQAYAL"
BASE COUNT         1482 a          940 c         1216 g         1585 t
ORIGIN      
        1 ttgtcaactt gccttgatga actccagttc tatcttcgac atcgttttct aggctacttt
       61 tgattttcat tgagtataac gtctatcaat tgttgcttat tgaataaagc ggcaaatgat
      121 aggttataga tttgtaaagg agatatatgt ttcgattaat ttttgactat atcaagcgtc
      181 ataagtggct ctatctattg gttgctgtga ccttgattat ttatgacgcc acacttttgc
      241 taccaacgca aatcattcag cggatggtag atatactaac caagaatgaa ttaacacagg
      301 ccatattggt tcaggagatg accttattgt tattggtaac agtactgaac tatgcaacag
      361 ccttcatttg gcatctgaaa ttatttcagg cttctgtcaa tttcaagttt gatatgcagc
      421 aacgtgcctt taaaaaatta gtgaccatgc ggactccatt ttatgaaaaa ttccgctcag
      481 gagatgtcat gactcgtttt tcaacggatg tggatggcct gatggagatg gtgggttacg
      541 gtcttatgat tgtcgtctat gcaggcggta tgttggcttt tatcattccc acgatgttct
      601 ttattgattg gaaaatttcg ctagtagctt tactaccgat gctgttcatg acactatgta
      661 tattttttat cgggagaaaa caagataagg cgattgatgc caatagagaa gctgtagccc
      721 agctcaataa tgaagttttg gaggtcatcg aaggcattcg agtgactcgg gcttatagta
      781 aaaaagcaac tcaaaaggct caatttcaag ctcgtaccaa gcaattggct caaggtgggg
      841 atcgcatcac ttccttgcaa tcattgtata atccattagc cacagtctgt ttgggattgt
      901 ctactatttt cgtgcttctc atgggggcac aagccgtgaa agcagggcaa ctgaccttgg
      961 gacaagtaat tgcccttcaa ttgtatgtcg gttcgctttt ggagccgttt tggacactag
     1021 cagatttcat ccttgtttat cagactggta agacatcttt cgaaaaactg caagagctga
     1081 ttgagacggg tgatgatttg gaggctgatg gttctaagga aattgcagag ctgtctagta
     1141 tttccttcaa aaattatagc tttagttatc cacaggcgga aagagccagc ctacaggaca
     1201 tcaattggac actgaaagca gggcaaactg taggtatcgt tgggaaaact ggttcgggaa
     1261 aaacgacctt ggttcgccaa ttcttacgcc agtatccgat tggtcaggga aattttttca
     1321 tcaatcatca atctgtttta gactttaaac gttctagtat agaggaaaaa attggctatg
     1381 ttccccaaga acacattttg ttttcaaggt ctgttggaga aaatattgcc cttgggaaag
     1441 tagctagtag ctctgaggag attgaacaag ccattgccac agcagctttt agtcaagatt
     1501 taaaacggat gactgatggt cttgatacca tgattggtga gcgtggtgtg tctatttctg
     1561 gcggtcagaa acaacggatt tccattgccc gtgctttcct gcgagagcca gatttgctga
     1621 ttctagatga ttctttgtcg gcagtggatg ctaggacaga acgccagatt atccaaaata
     1681 ttcaaaaaga acgtgcaggc aaaaccaatg tcatcgtgac ccatcgtcta tctgctgtca
     1741 accatgcaga ctgggtacta gtcttggatg aggggcgtat cgttgaggaa gggcgtccag
     1801 ctgatttgct tgcccaaaga ggttggtact atgaacaata ccaacgacaa caaagtcagg
     1861 aaggaggaga gtaatgacta ttttaggttt tttattaaaa caaatccgtc gtgttaagtg
     1921 gctatttata gtagctgtcg gcttctatct attggcttcg accatggttc gattggctcc
     1981 tctactgatt cagcaggcga ttgatgggcc gattacggat ttgagcaagg gtttgccttt
     2041 tgatgaagct gttttcttaa accagtcagc tcaatacatg ggaatgatta ttctgggagc
     2101 tattggcttc tacttatcta tgcgtctact catgcattgc gccaatcgta ttgctgaaaa
     2161 tctacgtaat caagcttatg atgttatgca acggttgcct atttcttatt ttgatgataa
     2221 gcccgctgga aaaattgcaa ctagaattgt caatgataca gaaactctgc ggacacaatt
     2281 ttatgggacc ttagttaatg cctttaacaa tattgttcgt ttgctcttta catacggtgt
     2341 cctattttac atgaatagga gtcttggttg gctgatgtta ctcttgatac cactctatat
     2401 cggtattcaa tttgcttaca agaaaatgac agacaagccg atgaaggatt tttatgatgc
     2461 aaggagtgac gtcaatacgc aggtgaatga gaccatgaat ggtgcaagtt tgattcaact
     2521 ttttggacaa gaagagcgca tcatgaaaga atttgaagtg actgctgaca agatgcgtcg
     2581 ggcagataat aagattatct gggcccagtc tcttgccacg tggaatctga gtggttttct
     2641 tcaaaatttg gtcattgctg cgattttaac ggtagtcggc tatcagtttc ttgcgggaca
     2701 gtatggagtt acagctggga aactctttgt ttatgtcaac tatatcgaag gagtcttcat
     2761 tgctttggga gcgctggttc aacagttccc aaatatgctt cgctcgtttg aaacaggtaa
     2821 gcgtctaatg gcccttctag aagaagaggt ggaggacgac tgtgatcgta ttttggaagt
     2881 tgaacaaggg caagtcgtgt ttgaacacgt gaactttagc tatgaagaaa acagaccagt
     2941 tttgaaagat attaccattc gggcagaaaa aggagaaaca gttgctctgg ttgggcatac
     3001 tgggtctggt aagtcttcta ttatgaacct cctctatcgt ttctatgatc cacaggaggg
     3061 acgtgtactg attgatggga aaaacatccg tgattattct cgtgagagcc tgcgcagtca
     3121 catggggatt gttttgcaag atccctatct ctttacaggg acgattgcta gcaatgtttc
     3181 catgaatgaa gaggaagcag atagaacaag aatcatgcag gcgttagaaa aagttggtgc
     3241 agggcctatg ctatctcgct tagagaaagg gattgatgaa ccggttgttg agaagggggc
     3301 ggccttttca agtggggaac gtcagttgat tgcctttgcg cggacactct actctgatcc
     3361 taaaattctg atattggatg aagcaacttc tcatattgat acggagacgg aagaaattat
     3421 ccaacatgct atggaagtgg tgaaagaagg tcggacaacc tttatcattg cccaccgact
     3481 ttctaccatt caaaatgcag accagattct tgtcttagat caaggccgaa ttattgaaca
     3541 tgggaagcat gaagaattgg ttgcacgtgg tggagtatat gctcaaatgc atgagattca
     3601 agcaaaggtg taataagcag ttaaaaattc caagctttgg ctggaatttt ttgctataat
     3661 tgactcatga aagccttaat ttttgatgta gatgatacgc tttatgatca gattcaaccg
     3721 tttgaacgtg ctttggaaag acacattgaa gtagcaaggg agcaaattga accactctac
     3781 ctttcctttc gcagatatgc ggatgaagta tttgaagcga cagcaattgg aaagatgagt
     3841 ctgaaagaca gtcatattta tcggatgaaa catgctttgg cagattttgg gtatcaggtg
     3901 tctgatgcaa cagccttagc tattcaaata gattatgatt attttcaagg gcagatagag
     3961 ctcagtccag tgtttccaga aatattttca tggtgtcagg cgcaaggaat tgcaatgggg
     4021 attattacga atggacctta tagacatcag ttgagaaaga tacgcacgat ggggttagtt
     4081 aattggtttg agctagaaca tgtcctaatt tctggacaag ttggtatcac aaagccaaat
     4141 cctgctattt ttcaactaat ggaagaacgt ttggggatgt caggcgaaga tatttgttat
     4201 ctaggggatt cgtttgaaaa tgatgtggta ggagcgaaag ctgcgaattg gaaggcaatt
     4261 tggtttaatc atagaaagag agtagagcca acagctccgt atcaggcaga taaagtggtg
     4321 acagattggg atgaactggt cgaagttatc cagtctttct agtggtttct acaagggatt
     4381 atcaattttt tataggtgtg ataggtaaaa tatattagca ggatgcctgt ctttatgata
     4441 gtataaaatg caagcataga agtataggtg tgaatccaag cagattttta ttcctactct
     4501 atgaaatttt ttgaaatgga gttacctatg acacaagctt tctttcctat ccatactcta
     4561 gagactgtta gtccagaatt acgagaaaac cttgcgactg tcaagaaaaa taatggcggt
     4621 tatattccta atctcatcgg tcttttagcc aattctccta cagccctaga aacctaccaa
     4681 actgttagtg gtatcaatcg ccgtagtagt ctgaatccga cggagcgtga agtggtacaa
     4741 attacagctg ccgttgccaa tggctgtgga ttttgcgtgg caggtcatac ggctatttct
     4801 atcaagcagg ttaagatgcc tgatgttatt ctacaggctc tacgccaggg aactcccatc
     4861 gaaacggatg caaaattgga tgctctcgct cgatttactc tggctgttat ccatgaaaaa
     4921 ggcaaggttg aacagccctt gctagaggaa tttttccaag agggctacac ggctgaaaat
     4981 gccttggatg tggtgttagg agtcagtctt gcaactcttt gtaattatgc caacaatctc
     5041 atcaacactc ctatcaatcc agaattacag gcctatgctc tgtaggagtt ccagcagaat
     5101 atgtatcgca aactagttag gacgtttaca ttatcctaac tagttttttt gaattaacca
     5161 agtgtgattt tcctaatata catttgatac tcttcgaaaa tcaaaattat ccgttgtcaa
     5221 ctt
//
LOCUS       DAZQCJ010000244          993 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Streptococcus suis isolate 4057-69_S69_L001
            SAMN35340958-rid23464883.guided.196, whole genome shotgun sequence.
ACCESSION   DAZQCJ010000244 DAZQCJ010000000
VERSION     DAZQCJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN35340958
            Sequence Read Archive: SRR24717193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002393609.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002393609.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:21:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,216
            CDSs (total)                      :: 2,172
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 28 of 52
            Pseudo Genes (incomplete)         :: 25 of 52
            Pseudo Genes (internal stop)      :: 15 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN35340958-rid23464883.guided.196"
                     /isolate="4057-69_S69_L001"
                     /host="pig"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            46..783
                     /gene="erm(B)"
                     /locus_tag="ACHU66_002216"
     CDS             46..783
                     /gene="erm(B)"
                     /locus_tag="ACHU66_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFR3390441.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          356 a          173 c          171 g          293 t
ORIGIN      
        1 acttctgtat ttgtttatag atgtaatcac ttcaggagtg attaaatgaa caaaaatata
       61 aaatattctc aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg
      121 aatttaaaag aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg
      181 aaactggcta aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac
      241 ttatcgtcag aaaaattaaa actgaatact cgtgtcactt taattcacca agatattcta
      301 cagtttcaat tccctaacaa acagaggtat aaaattgttg ggaatattcc ttaccattta
      361 agcacacaaa ttattaaaaa agtggttttt gaaagccgtg cgtctgacat ctatctgatt
      421 gttgaagaag gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg
      481 cacactcaag tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa
      541 ccaaaagtaa acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa
      601 tattggaagc tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg
      661 tttactaaaa atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc
      721 gttacttatg agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa
      781 taattctatg agtcgctttt gtaaatttgg aaagttacac gttactaaag ggaatgtaga
      841 taaattatta ggtatactac tgacagcttc caaggagcta aagaggtccc tagcgcctac
      901 ggggaatttg tatcgataag gggtacaaat tcccactaag cgctcgggac cccttgtagg
      961 aaaatgtcct aagtgtggca acaatattgt att
//
